Mitochondrial respiration in different cell lines measured in the 2.0 mL and 0.5 mL chambers of the O2k-FluoRespirometer (Oroboros): an experimental basis for platform comparison with the Seahorse XFe24 Bioanalyzer (Agilent)

The home institution is the institution / organisation where the STSM applicant holds their primary affiliation as registered on their e-COST profile / STSM application / C.V. and where they are currently performing their main strand of research.

The host institution concerns the institution / organisation that will host the successful applicant.

Funding regulations

STSM in which practical science is getting carried out.

STSM which directly lead to tangible outcomes (e.g. writing papers/grants).

The money will be returned AFTER the Grantee has completed her/his visit and submitted a report (a written report on the activities carried out during the STSM within 30 days of the end of the research visit)

Researchers from Participating *Inclusiveness Target Country (ITC)can receive 50% of the grant upon completion of the 1st day of the STSM. The remaining 50% will be paid after the Grantee has completed her/his visit and submitted a report.

Note: STSM grantees must make their own arrangements for all provisions related to personal security, health, social security and pension matters.

Funding regulations

50-70% of the STSM budget will be devote 5to complete specific WG tasks towards a manuscript. Participants will be mainly invited to apply for STSM to join such workshops.

30-50% of the STSM budget will be still available for training activities.

How to apply for a STSM

Interested researchers have to submit their application and supporting documents to the STSM Coordinator:Magdalena Labieniec-Watala

Supporting documents

Letter of invitation to the applicant from a senior researcher affiliated to the host institution.

The submitted STSM application form (downloadable when the online application is submitted – see point 4 below).

Motivation letter including an overview of the proposed activities that will be performed which must contain a plan of work for the visit highlighting the proposed contribution to the scientific objectives of MitoEAGLE.

Letter of support from the home institution.

Full C.V. (including a list of academic publications – if applicable).

Application process

Read the funding rules in Section 8 of the COST Vademecum: »Guidelines

Include in your application a letter of invitation from the host institution confirming that the host can undertake the STSM on the given dates. The final dates of the STSMs have to be negotiated between the applicant and the host.

Evaluation process

The amounts granted for each individual STSM will be determined during the evaluation process by the STSM Coordinator, Chair and Co-chair.

The selection of applicants is based on the scientific scope of the STSM application which must clearly compliment the overall objectives of MitoEAGLE outlined in the Memorandum of Understanding.

Within 30 days from the end date of the STSM, the successful applicant must submit a scientific report to the host institution and to the STSM Coordinator. The Scientific Report must contain the following:

confirmation by the Host institution of the successful execution of the STSM

Failure to submit the scientific report within 30 days from the end date of the STSM will effectively cancel the Grant.

Please note that the COST Association can request additional information to substantiate the information contained within the documents submitted by STSM applicant.

General: STSM duration and budget

Must have a minimum duration of 5 calendar days that includes travel.

STSMs need to be carried out in their entirety within a single Grant Period

The financial support is a contribution to the overall expenses and does not necessarily cover all expenses. Financial support is limited to cover travel, accommodation and meal expenses and is paid in the form of a Grant.

Up to a maximum of EUR 160 per day for accommodation and meal expenses

Up to a maximum of EUR 300 can be afforded for travel costs

Up to a maximum of EUR 3500 in total can be afforded to each successful applicant

Financial support is limited to cover travel, accommodation and meal expenses and is paid in the form of a Grant.

Specific provisions have been introduced to enable researchers from ITC participating in the COST Action to request a pre-payment of 50% of their STSM Grant when they complete the first day of their STSM. In such case, the representative of the Host Institution must confirm by e-mail to the Grant Holder that the STSM applicant has officially started the mission on day 1. Only then the Grant Holder can arrange the payment of 50% of the STSM grant. The remaining 50% of the Grant is payable once the administrative requirements have been satisfied after the STSM.

MitoEAGLE host institutions

We would like to invite institutions who are willing to host MitoEAGLE Short Term Scientific Missions to join the list as an important information for potential applicants. Please, let us know the topics of expertise of your institution related to the MitoEAGLE Working Groups and Memorandum of Understanding, and the contact details of the responsible mentor.

Experiments are mainly performed on isolated mouse cardiomyocytes (WT and KOs). We have been using earlier rat and trout cardiomyocytes as well. As a preparation we use either intact cells or saponin-permiabilized cells. From the techniques routinely used in the lab: oxygraphy (Strathkelvin with custom software); absorbance and fluorescence spectrophotometers; confocal [imaging and FCS/RICS] and fluorescence microscopy. Fluorescence microscopy is running on our custom software allowing us to combine fluorescence imaging with single cell mechanics (control over cell mechanical contraction via carbon fibers) and patch clamping (electrophysiology). [WG2]

In addition to wet lab, we have experience and are actively developing mathematical models of different aspects of heart function: bioenergetics, mechanical contraction, and electrophysiology [WG2]. Finally, all experiments are monitored via database developed in the lab. [WG1]