TY - THES
T1 - Combined assessment of drug dissolution and epithelial permeability : implementation of online TEER measurement and extension to BCS class III and IV compounds
A1 - Mündörfer,Marco
Y1 - 2010/12/27
N2 - Measurement of drug dissolution and permeation of solid oral dosage forms in a combined experiment is a quite obvious research approach that nevertheless has only been sparsely considered up to now. In the present work an existing apparatus that has been developed for the mentioned purpose has been adapted to comply with the requirements for the analysis of low permeable compounds of BCS classes III and IV. Next to that, the apparatus was developed further in a way that the course of transepithelial electrical resistance (TEER) could be recorded throughout conduction of an experiment. Implementation of this tool was supposed to provide for the opportunity to survey and demonstrate the integrity of a Caco-2 cell monolayer throughout an experiment. Furthermore, it was of interest to evaluate in how far the novel method was suitable for the analysis of the influences of excipients, as e.g. EDTA, on the permeability of the paracellular pathway. Finally, it was pointed out that the revised apparatus is able to analyze Lasix® 40mg furosemide tablets correctly. Hence, the research approach has advanced significantly to the aim of the establishment of an in vitro method for the targeted formulation development of low permeable compounds.
KW - Permeation
KW - Resorption
KW - Pharmazeutische Technologie
KW - Zellkultur
CY - Saarbrücken
PB - Saarländische Universitäts- und Landesbibliothek
AD - Postfach 151141, 66041 Saarbrücken
UR - http://scidok.sulb.uni-saarland.de/volltexte/2010/3503
ER -