I used to do UV crosslinking for plaque lifts on nitrocellulose all the time
and had good luck.
In article
<Pine.A41.3.95b.961204105651.154004A-100000 at homer35.u.washington.edu>,
yoonpark at u.washington.edu says...
>>Hi, everyone,
>>I'm trying to colony hybridization using nitrocellulose membrane (S&S).
>According to the manual which the manufacturer provided, vacuum drying oven
>should be used to fix DNA onto nitrocellulose, but UV cross-linker can be
>used for nylon membrane. Is it true that UV cross-linker cannot be used
>for nitrocellulose membrane for this purpose? If it's, why?
>>If someone knows about this, can you explain it to me?
>Thanks in advance!
>>YP.
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>Yoonsuk Park, Ph.D.
>University of Washington e-mail) yoonpark at u.washington.edu>Dept. of Oral Biology, Box 357132 Phone) 206-543-5477 (ext. 40)
>Seattle, WA, 98195-7132 Fax) 206-685-3162
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