> Try feeder cells (normally isogenic splenocytes work great)
(more stuff deleted)
>I agree with trying feeder cells. Another option (one which gave me what I
>calle
>d the "Lazarus clone") is to use conditioned medium- I believe we used 0.2
>micro
>n sterile filtered medium from a Buffalo rat liver cell line. Thanks to Dr.
>Dwan
>e Wylie for the original suggestion of trying this.
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>David J. Meyer
I have also tried to use conditioned medium.
The best was that from 5637 human bladder carcinoma cell line.
It also produces LIF as well as a bunch of other cytokines.
Andrei