Dear Netters:
For proteinase preparation, it is mentioned in Molecular Cloning(by
Maniatis) that pronase needs a preatment to remove any DNase or RNase
activity, but this pretreatment is not required for proteinase K. Is
there any reason that proteinase K is not required for the pretreatment?
Someone said to me that proteinase K digested the DNase or RNase very
quickly and is not required for pre-treatment; but this is just a
thought. IS anyone there can tell me any citation concerning this
thought?
any thoughts are welcome.
regards,
Chi-Kuang
Purdue University