Mutation Discovery Using High Resolution Melting

Mutations in PCR products are detected by the presence of heteroduplexes optimally detected by our LCGreen® Plus dye. Variations are identified by changes in the shape of the melting profile compared to a reference sample. Hi-Res Melting® (HRM) is being used for mutation discovery in tumor samples, yielding information on mutations present in low allele fractions (as low as 5%) and allelic imbalances between tumor and matched normal samples.

BioFire has been a leader in Hi-Res Melting technologies and continues to make high quality, reliable LCGreen melting dye and LightScanner master mix that are specifically designed for HRM. Both of our LCGreen Plus melting dye and our LightScanner Master Mix are compatible with all instruments featuring HRM capabilities.

Use the links below to learn how your research can get you there faster with our line of HRM products.