RE: [Histonet] Microwave retrieval new question

From:

"Connie McManus"

I find this all interesting as my pathologist has suggested that I try
the MW for our AR procedures. We were thinking about using it for
proteinase K rather than using a citrate buffer or Tris. I would assume
that the temp would be very important to control with the enzyme. So...
For those of you who do MW AR, would you mind sharing your procedure?
Thnks bunches!! *G*
Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone: 435/797-1891
fax: 435/797-2805
email: convmcm@cc.usu.edu
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems,
Joyce
Sent: Thursday, October 21, 2004 5:15 AM
To: marjorie lehman; Amos Brooks; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Microwave retrieval
We were fortunate enough to have some left over capital dollars and have
purchased the new Biogenex Retreival Microwave. It is wonderful - saves
lots of time and gives consistency in staining! The only thing we would
change is to be have smaller containers for instances when we have just
a few slides to retreive. But we think its a winner for those of you are
looking to purchase it.
Cheers,
Joyce
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of marjorie
lehman
Sent: Thursday, October 21, 2004 7:19 AM
To: Amos Brooks; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Microwave retrieval
Here is a Second Opinion!
I tried using a (Household) microwave for a long time and was not happy.
After
I got my Decloaking Chamber from Biocare I never looked back!
Marge
-----Original Message-----
From: Amos Brooks [SMTP:amosbrooks@earthlink.net]
Sent: Wednesday, October 20, 2004 8:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microwave retrieval
Ed,
I strongly recommend that you don't try to put a coplin jar of
retrieval solution with slides in it in the microwave and expect any
consistant results. What happens with this method is you get a spike of
heat then a temperature drop. Very often there is boil over and your
slides
dry out. Ideally you should be looking for an even temperature for a
defined length of time that can be well monitored.
For this reason if you *must* use a microwave it should be a
lab
grade oven that has consistant wattage and temperature control. It would
also be good to use one of the microwave pressure cookers to improve the
consistency. (I think Biocare Medical has them ... the guys that sell
Borg
Decloaker).
My ultimate recommendation is to stick to the tried and true
waterbath or steamer method. It may take a bit longer but the
consistancy
is better and there is far less variables in the procedure.
Just my opinion,
You know what they say 'bout opinions,
Amos Brooks
At 01:00 PM 10/20/2004, you wrote:
>Message: 10
>Date: Wed, 20 Oct 2004 11:30:26 -0400
>From: "Harris, Ed"
>Subject: [Histonet] Microwave retrieval
>To: "'histonet@lists.utsouthwestern.edu'"
>
>Message-ID:
>Content-Type: text/plain; charset="iso-8859-1"
>
>Can anyone give me the pro's and con's of steamer/pressure (Decloaker)
vs.
>microwave retrieval ?
>Our Doc wants us to change from the Decloaker to microwave. Is a
household
>microwave adequate or do we need a lab grade unit?
>Does the microwave save time and improve turn around time?
>
>Thanks in advance
>Ed Harris HT ASCP MT ABB
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