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Protocols - Immunofluorescence: Rabbit Anti-Murine RELMα

The following protocol used B6 mice that were infected with 200 embryonated Trichuris muris eggs and sacrificed 15 days post-infection. The tip of the cecum was removed, rinsed in 1X PBS pH 7.2, and fixed in 4% PFA. Following fixation, the tissue was embedded in paraffin and cut into 5 μM sections.

Incubate the tissue section with a fluorescent conjugated secondary antibody for 2 hours at room temperature. Wash the slide twice for three minutes.

Counterstain the tissue section with DAPI.

The following protocol used B6 mice lung sections that were injected with helminth Schistosoma mansoni eggs. Inflamed lung sections were fixed in 4% PFA. Following fixation, the tissue was embedded in paraffin and cut into 5 μM sections. Some tissue sections or cell preparations stained were from day 14 bleomycin-instilled mice.