Enzymic Determination of Methylguanidine in Urine

Abstract

Methylguanidine is known to accumulate in the body fluids of uremic patients1 , 2 and has proved, to be a uremic toxin3. Determination of its concentration in body fluids would, therefore, be useful in clinical practice. Recently, the determination of methylguanidine has been carried out by means of automated high-performance liquid chromatography with a fluorometric detection method for guanidino compounds4. The guanidino compounds analyzer is well suited for the simultaneous determination of several guanidino compounds. However, it is very expensive and inappropriate for analyses of many samples. In clinical practice, the enzymic determination of metabolite concentration has been widely employed because of its simplicity and specificity.