DSpace Community:http://ir.yu.ac.kr/handle/YU.REPOSITORY/209242018-12-19T09:55:35Z2018-12-19T09:55:35ZEnhancement of DNA mediated energy transfer by Mg2+조태섭장윤정김종문[김종문]이현미[이현미]Biao Jin[Biao Jin]http://ir.yu.ac.kr/handle/YU.REPOSITORY/337362015-12-17T05:09:42Z201501-01-01T00:00:00ZTitle: Enhancement of DNA mediated energy transfer by Mg2+
Author(s): 조태섭; 장윤정; 김종문[김종문]; 이현미[이현미]; Biao Jin[Biao Jin]
Abstract: The excited energy of ethidium transferred to meso-tetrakis(N-methylpyridinium-4-yl) porphyrin (TMPyP) when they are simultaneously intercalated between DNA base-pairs. The minimum number of base-pairs between ethidium and TMPyP required for excited ethidium to emit a photon was 36 angstrom or 10.6 base-pairs. The distance was extended to 72 angstrom or 21.2 base-pairs in the presence of 100 mu M Mg2+ ions although the conformation of TMPyP complexed with DNA, which was investigated by circular and linear dichroism spectroscopy, was unaffected by the presence of Mg2+ ions. (C) 2014 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.201501-01-01T00:00:00ZBinding Geometry of Free Base and Mn(III)meso-Tetrakis (N-methylpyridium-4-yl)porphyrin to Various Duplex and Triplex DNAs조태섭성기웅신종헌장명덕한성욱[한성욱]이동진[이동진]김래영http://ir.yu.ac.kr/handle/YU.REPOSITORY/335602015-12-17T05:05:34Z201502-01-01T00:00:00ZTitle: Binding Geometry of Free Base and Mn(III)meso-Tetrakis (N-methylpyridium-4-yl)porphyrin to Various Duplex and Triplex DNAs
Author(s): 조태섭; 성기웅; 신종헌; 장명덕; 한성욱[한성욱]; 이동진[이동진]; 김래영
Abstract: The binding mode of free base and Mn(III) meso-tetrakis(N-methylpyridium-4-yl) porphyrin (TMPyP and MnTMPyP) to various duplex and triplex synthetic polynucleotides was investigated by polarized light spectroscopy, specifically circular and linear dichroism (CD and LD) spectroscopy. Both TMPyP and MnTMPyP produced a similar positive CD spectrum in the Soret absorption region when bound to poly(dA)center dot poly(dT) duplex and poly(dA)center dot[poly(dT)](2) triplex. Considering that the third poly(dT) strand blocks the major groove, this result suggested that both porphyrins bound near the minor groove where the axial ligands of the central Mn(III) ion did not affect the binding mode. In contrast, TMPyP intercalated and Mn TMPyP bound the exterior of poly(dG)center dot poly(dC) and poly(dI)center dot poly(dC) duplexes, as judged from the negative and positive CD signals, respectively. The CD signals for both intercalated TMPyP and externally bound MnTMPyP were greatly altered when bound to poly(dG)center dot poly(dC)center dot poly(dC)(+) and poly(dI)center dot poly(dC)center dot poly(dC)(+) triplexes, indicating that the binding modes of both porphyrins were affected by the presence of the third strand, poly(dC)(+), which contrasted with the AT polynucleotides. This observation supported the conclusion that the external binding site of MnTMPyP at the GC and IC duplexes is the major groove. The reduced LD spectrum indicated that the angles of the two electric transition moments of both TMPyP and MnTMPyP in the Soret absorption region were 57 degrees and 65 degrees with respect to the local DNA helix axis when bound to the poly(dA)center dot[poly(dT)](2) triplex. On the other hand, a strong, wavelength- dependent reduced LD was observed in the Soret region for both porphyrins when associated with CGC triplex, and the excessive tilt degree prevented any reasonable calculation of the angles.201502-01-01T00:00:00Z‘세계문학’의 상상과 번역ー우치다 로안의 번역과 근대 일본의 내셔널리즘ー최범순http://ir.yu.ac.kr/handle/YU.REPOSITORY/333912015-12-17T05:03:21Z201401-01-01T00:00:00ZTitle: ‘세계문학’의 상상과 번역ー우치다 로안의 번역과 근대 일본의 내셔널리즘ー
Author(s): 최범순
Abstract: Uchida Roan is a person who translated the revival of Tolstoy and Crime and Punishment of Dostoevsky for the first time in Japan. So when discussing Uchida Roan's translation, focus is placed on the two works. However, Uchida Roan translated many foreign literatures during the late Meiji twenties. But there should not be mentioned most of these translations. In this paper I have attempted to clarify the significance with the translation of Uchida Roan in the late Meiji twenties from the side of the nationalism. Specifically, I paid attention to the translation “voice of heaven” of Voltaire works and “Senjin” of Zola works.
The both of “voice of heaven” and “Senjin” had a strong sense of the Sino-Japanese War. The only retouched part which was seen in the process from “giant story” to “voice of heaven” refers to the fact that Uchida Roan was looking critically the Sino-Japanese War. The postscript of “Senjin” told that it was going to ring the alarm bell to so-called “people”. Translations of two included a critical message against Sino-Japanese War.201401-01-01T00:00:00ZNK2-specific domain is responsible for cell death upon ectopic expression of VND in various Drosophila tissues유시욱이종철김건희이윤정http://ir.yu.ac.kr/handle/YU.REPOSITORY/332532015-12-17T05:01:35Z201402-01-01T00:00:00ZTitle: NK2-specific domain is responsible for cell death upon ectopic expression of VND in various Drosophila tissues
Author(s): 유시욱; 이종철; 김건희; 이윤정
Abstract: Drosophila ventral nervous system defective (VND) is a transcription factor containing tinman domain, homeodomain, and NK2-specific domain. The VND plays an essential role during the development of embryonic nervous system, however, little is known about its roles beyond embryonic stage. In this study, the expression patterns and levels of the VND during larval and adult stages were examined by using GAL4/UAS binary expression system and real-time PCR. The VND protein was expressed in larval brain, eye, and wing imaginal discs, implying possible roles of this protein in the larval tissues other than embryonic nervous system. Gain-of-function phenotypes of vnd were analyzed by crossing various GAL4 drivers with UAS-vnd transgenic line. Interestingly, we observed the massive cell death in the tissues including eye and wing discs where GAL4 is expressed. To down-regulate endogenous vnd transcripts in the eye disc, UAS-vnd-IR (inverted repeat) was crossed with ey3.5-GAL4 line. Loss-of-function phenotypes of vnd also revealed the black ommatidial spots indicating apoptotic cell death in the transgenic eye tissues. To identify a domain within the VND involved in apoptosis, VND mutant proteins were ectopically expressed in the eye disc. In contrast to VND-Delta tinman and VND-Y54M mutant proteins, overexpression of VND-Delta NK2 did not lead to cell death, indicating that NK2-specific domain is responsible for apoptosis. Taken together, our results suggest that VND might be involved in the development of the various tissues after embryonic stages and in apoptosis via NK2-specific domain.201402-01-01T00:00:00Z