Buprenorphine: purchased from the Western University animal care and veterinary services facility as a stock solution of 0.3
mg/ml; the stock is then diluted in normal saline to a 0.012 mg/ml working solution (both the stock and the working solution
are stored at room temperature)

NOTE: Perform 42‐day in vivo protocol as described in protocol 4. The following alternate protocol explains an adapted transplantation procedure on day 10 only.NOTE: For additional details and photographs of the intrapancreatic injection surgical procedures please refer to Figure .

Support Protocol 3: Mouse Euthanasia and Tissue Dissection

Materials

Mice that have been subjected to EdU injection and glucose tolerance test (protocol 4)

This paper demonstrates that sequential transplantation of MSC followed by ALDHhi cells improved hyperglycemia and glucose tolerance by increasing beta cell mass via stimulation of islet‐regenerative and revascularization program