Load sample

Load 200 μl of the priming mix into the flow cell via the priming port (not the SpotON sample port), avoiding introduction of air bubbles

Mix the prepared library gently by pipetting up and down prior to loading

Add 75 μl of sample to the flow cell via the SpotON sample port in a dropwise fashion. Ensure each drop flows into the port before adding the next

Gently replace the SpotON sample port cover, making sure the bung enters the SpotON port, close the priming port and the MinION lid

Protocol: Software setup

MinKNOW

MinKNOW is the program needed to connect your computer with MinION. It has a graphic user interface for configuring and running the sequencing. Furthermore, it has integrated base-caller software to convert the raw nanopore signals to the strings of nucleotides, in fastq format.

Installation:

Select your host operating system and follow the link to the installation instructions.

Since the supported systems and the installation procedure is constantly updated, please always refer to the community website for details.

Starting the software:

Requirements:

You have to have a account at nanopor MinION to start the software

The divice need to be attached to the computer to start the software

Either you find the “MinION Software” botton directly or you open it via the terminal using the path “/opt/ui/MinKNOW”

Launching MinKNOW and running the sequencing

After having the software installed, the major steps would be connect, initialize and configure the MinION within the MinKNOW software. Afterwards the device would be ready for loading and sequencing the data.

Again please refer to the community web site for up-to-date instructions and follow the graphically enhanced manuals.

This is the current link to the manual for starting MinKNOW and the follow up procedures. https://community.nanoporetech.com/protocols/experiment-companion-minknow/v/mke_1013_v1_revan_11apr2016/introduction-to-local-base

Since the URL is not generic, it would be best to find the latest manual from the getting started page here: https://community.nanoporetech.com/getting_started