Consumables

PacBio provides complete workflows and all the consumable components you need to perform Single Molecule, Real-Time (SMRT) Sequencing. Regardless of project size, the consumables promote customization while maintaining cost-effectiveness. Due to the flexibility of the PacBio sequencing platforms, these components support a variety of workflow steps and research goals. Long, accurate sequence reads are only a few steps away.

Sequel

Sample and Template Preparation Kits

DNA (genomic DNA or amplicons) and cDNA camples are converted into a primer-annealed SMRTbell library format. May be performed manually at the benchtop or by an automated liquid-handling system.

Binding and Cleanup Kits

Primer-annealed SMRTbell tamplates are bound to a DNA polymerase in preparation for sequencing. Polymerase-bound SMRTbell templates are annealed to magnetic beads for loading onto the PacBio systems.

SMRT Cells, Sequencing Reagents Kits, and Accessories

Disposables

Multi-well plates, septa, and tips specifically designed and optimised for use with the PacBio RS II System.

Target Enrichment

Target enrichment through a solution-based capture system enables enrichment of defined regions of interest up to several Mb in size. Roche NimbleGen's SeqCap EZ enrichment technology, combined with PacBio's SMRT Sequencing, has demonstrated the ability to use 6 kb fragments and is applicable to practically any type of SeqCap EZ panel. They have demonstrated efficient enrichment for the Major Histocompatiblity Complex and a comprehensive cancer gene panel. On average, 61% of the reads were on target and an average enrichment factor of 1300x was obtained. With PacBio long reads, heterozygous SNPs were used to phase the reads and generate haplotypes.

Features

1000-fold enrichment.

Full-gene capture - exonic and intronic regions.

Phase variants to obtain haplotypes.

Multiplexing Amplicons

Direct PCR amplification of a specific region is a powerful technique for performing targeted sequencing. When combined with multiplexing capabilities, PCR amplification is an efficient approach to studying many samples. PacBio offers two barcoding strategies for multiplexing. In the first approach, barcodes are added to the end of the amplicon during PCR, and the standard SMRTbell adapter is subsequently ligated onto the barcoded amplicon.