1,3-Dicyanobenzene

1,3-ジシアノベンゼン

[CAS No. 626-17-5]

m-Phthalodinitrile

m-フタロジニトリル

Molecular formula: C8H4N2 Molecular weight: 128.13

ABSTRACT

1,3-Dicyanobenzene was studied for oral toxicity in rats in a 28-day repeat dose toxicity test at doses of 0, 8, 40 and 200 mg/kg/day. Salivation, hypoactivity, bradypnea, soiled perineals, lacrimation and loss of hair were observed in males and/or females of the 200 mg/kg group. Body weights and food consumption decreased in males and females of the 200 mg/kg group during the early stage of the administration period. Thereafter, body weight gain and food consumption recovered in females, but their decrease continued to be recorded throughout the administration period in males. Urinalysis showed increase in urine volume and decrease in specific gravity in males and females of the 40 and 200 mg/kg groups, and decrease in pH in males and females of the 200 mg/kg group. Blood chemical examination showed increases in total protein, albumin, total cholesterol, phospholipids and calcium, and decrease in chloride in males and females of the 200 mg/kg group. Further, decrease in alkaline phosphatase in males receiving 200 mg/kg, and increases in glutamic pyruvic transaminase, triglycerides and glucose, as well as decrease in sodium in 200 mg/kg females were detected. Absolute and relative liver and kidney weights increased in males and females of the 200 mg/kg group, and relative liver weights also increased in males of the 40 mg/kg group. Absolute and relative weights of the adrenals increased in females receiving 200 mg/kg, and the relative adrenal weights were also increased in males given 200 mg/kg. Histopathologically, hypertrophy of hepatocytes was observed in many males of the 40 mg/kg group and all males and females of the 200 mg/kg group. The incidence of hyaline droplets in proximal tubules of the kidney was increased in males of the 8 mg/kg and higher dosage groups, and papillary necrosis, pelvis dilatation, basophilic change of proximal tubules and dilatation of distal tubules were also observed in one male of the 200 mg/kg group. In the recovery test, all changes observed during the administration period demonstrated decrease in their extent or severity. The NOELs are considered to be 8 mg/kg/day for females and less than 8 mg/kg/day for males.

1-Methoxynaphthalene was not mutagenic to Salmonella typhimurium TA100, TA98, TA1535, TA1537 and Escherichia coli WP2 uvrA, with or without an exogeneous metabolic activation system.

1,3-Dicyanobenzene induced neither structural chromosomal aberrations nor polyploidy in CHL/IU cells up to the limit concentration of 10 mM, in the absence or presence of an exogenous metabolic activation system.

Salivation, hypoactivity, bradypnea, perineal soiling, lacrimation and loss of hair were observed in males and/or females of the 200 mg/kg group. Body weight and food consumption decreased in males and females of the 200 mg/kg group during the early stage of the administration period. Urinalysis showed increase in urine volume and decrease in specific gravity in males and females of the 40 and 200 mg/kg groups, and decrease in pH in males and females given 200 mg/kg. Blood chemical examination showed increases in total protein, albumin, total cholesterol, phospholipids and calcium, and decrease in chloride in males and females of the 200 mg/kg group. Further, decrease in alkaline phosphatase in males receiving 200 mg/kg, and increases in glutamic pyruvic transaminase, triglycerides and glucose, and decrease in sodium in females given 200 mg/kg, were detected. Absolute and relative liver and kidney weights increased in males and females of the 200 mg/kg group, and relative liver weights were also increased in males of the 40 mg/kg group. Absolute and relative weights of the adrenals increased in females of the 200 mg/kg group, and relative adrenal weights were also increased in the 200 mg/kg males. Histopathologically, hypertrophy of hepatocyts was observed in many males of the 40 mg/kg group and all males and females of the 200 mg/kg group. The incidence of hyaline droplets in proximal tubules of the kidney increased in males of the 8 mg/kg and higher dosage groups, and papillary necrosis, pelvis dilatation, basophilic change of proximal tubules and dilatation of distal tubules were also observed in one male of the 200 mg/kg group.

The NOELs are considered to be 8 mg/kg/day for females and less than 8 mg/kg/day for males.