now you see it now you don't

Rob Harling wrote:
> PCR works if we use freshly - prepared DNA, ie extracted from the
> bacteria then run on the same day. If we store the DNA at 4C for
> only one week, then PCR fails, we get zilch in the lanes at any
> concentration of template. The DNA still looks the same after one week
> when run on a gel, ie there doesn't seem to be nuclease breakdown
> of the DNA. Does anyone know what's happening to the DNA in storage?
>Rob,
Do you know if it still cuts with restriction endonucleases?
Kevin
Dr Kevin O'Donnell "Work as if you were in the early days
Diagnostics and Molecular Biology of a better nation" Alasdair Gray
Scottish Agricultural Science Agency
Edinburgh odonnell at sasa.gov.uk