Long & Accurate PCR

Many of today's PCR based applications require longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase. Cloning and expression experiments, cDNA analysis and array work can be improved by using long and accurate amplification over routine amplification.

Long and Accurate (LA) amplification is achieved by combining a thermostable polymerase with a second polymerase exhibiting a 3'—>5' exonuclease activity. The exonuclease, or proofreading, activity repairs terminal misincorporations. This repair allows for greater read lengths and increased fidelity.

We bring you a complete line of Long and Accurate products for DNA research. Our AccuTaq LA and KlenTaq LA enzymes and blends provide increased yield, higher processivity for greater lengths, and proofreading activity for increased fidelity over other long and accurate enzymes.

An optimized mix of KlenTaq-1 and a proofreading exonuclease, these enzymes are the products of choice for amplifying DNA with difficult secondary structure or for applications requiring high yields and fidelity.

Generate amplicons up to 5 kb on complex templates or 20 kb on less complex DNA with higher fidelity (4X higher than standard Taq) using KlenTaq LA DNA polymerase

Combining an antibody based hot start mechanism with our long and accurate enzymes results in increased specificity and sensitivity to our high yield, high fidelity products. JumpStart AccuTaq is the ideal choice for high fidelity cloning applications or high yield array work.

Increased yields compared to other hot start polymerases, especially when amplifying templates with high GC content

JumpStart KlenTaq LA and competitors' high performance enzymes were used to amplify an 890 bp amplicon containing 80% overall G-C content from total human genomic DNA. Reactions were run according to supplier's recommendations both without (Lanes 1-5) and with (Lanes 6-10) suggested PCR enhancers.