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Abstract

An optical coherence microscope (OCM) has been designed and constructed to
acquire 3-dimensional images of highly scattering biological tissue.
Volume-rendering software is used to enhance 3-D visualization of the data sets.
Lateral resolution of the OCM is 5 µm (FWHM), and the depth
resolution is 10 µm (FWHM) in tissue. The design trade-offs for a 3-D
OCM are discussed, and the fundamental photon noise limitation is measured and
compared with theory. A rotating 3-D image of a frog embryo is presented to
illustrate the capabilities of the instrument.

Photodetector noise as a function of reference beam power. A spectrum
analyzer was used to measure the photodetector noise (solid circles) at
122.5 KHz with a bandwidth BW=3 KHz. The theoretical curve (solid line) is
calculated using this value of the bandwidth in equation (6).

OCM signal-to-noise ratio as a function of reference beam power. The solid
line is calculated from equation (8) with no adjustable parameters. The dashed
line includes an additional multiplicative constant of 0.40.

First frame of the movie of a frog embryo at stage 41 of development. The
movie is provided in two sizes: a 2.46 MB file and also a 385 KB version
with slightly lower resolution but faster download time.