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The Human ON-TARGETplus RTF G Protein-coupled Receptor (GPCR) siRNA Library includes siRNA reagents targeting genes encoding GPCRs, transmembrane proteins required for the transduction of extracellular signals from a wide variety of ligands, including neurotransmitters, hormones, and other small molecules. This siRNA Library does not include olefactory or taste receptors.

ON-TARGETplus modifications combine sense strand inactivation with a novel seed-region modification. This patented siRNA approach is the best strategy to prevent off-target effects caused by both the sense and antisense strands while maintaining high silencing potency.

Highlights

Six ready-to-use 96-well plate sets provided for two triplicate screens

Pre-plated, validated RNAi controls included

No aliquoting necessary, just resuspend and add cells

siRNA reagents provided in clear plates at 6.25 pmol per well (50 nM final screening concentration)

Black or white clear-bottom plates available to support assays involving fluorescent or luminescent detection

For a diagram of the RTF Library Plate layout, see Figure 1 on the Supporting Data tab.

Well

Pre-plated control

Control Catalog No.

A1

ON-TARGETplus Non-targeting siRNA #1

D-001810-01

B1

ON-TARGETplus Non-targeting siRNA #2

D-001810-02

C1

ON-TARGETplus Non-targeting siRNA #3

D-001810-03

D1

ON-TARGETplus Non-targeting siRNA #4

D-001810-04

E1

ON-TARGETplus Non-targeting pool

D-001810-10

F1

ON-TARGETplus Human Cyclophilin B control pool

D-001820-10

Experimental considerations

DharmaFECT Cell Culture Reagent (DCCR) is recommended for use with RTF libraries to dilute transfection reagents prior to use. DCCR may be purchased separately or accompanying your RTF Optimization Plates

Gene Targets

For a complete list of target genes in this siRNA Library please email Technical Support or call 1-800-235-9880. International customers please call 303-604-9499 or your local Sales Representative.

Hazardous

No

Shipping Condition

Ambient

Stability at Recommended Storage Conditions

At least 12 months

Storage Condition

4 C

Our siRNA knockdown guarantee

siGENOME and ON-TARGETplus siRNA reagents (SMARTpool and three of four individual siRNAs) are guaranteed to silence target gene expression by at least 75% at the mRNA level when demonstrated to have been used under optimal delivery conditions (confirmed using validated positive control and measured at the mRNA level 24 to 48 hours after transfection using 100 nM siRNA).

Note: Most siGENOME and ON-TARGETplus siRNA products are highly functional at 5 to 25 nM working concentration.

Plate layout of ON-TARGETplus RTF siRNA Libraries

Validated control siRNAs and pools are pre-dispensed into column 1 of each RTF Library plate, providing a consistent baseline for screening and assay efficiency.