PROTEIN REFOLDINGBaroFold conducts high-pressure refolding feasibility studies on all proteins, whether expressed from mammalian cells or E. coli. Having refolded approximately 100 proteins to date, BaroFold is highly knowledgeable in all redox, solution, and protein structural aspects of refolding. BaroFold conducts all feasibility studies in collaboration with the client, applying PreEMT technology and typically assessing yields in-house by employing analytical methods developed by the client. Applications include traditional refolds as well as pressure-treatment of final formulations to decrease aggregate content and reduce immunogenicity.

FORMULATION STABILITY AND IMMUNOGENICITY STUDIESBaroFold conducts pressure and thermal-accelerated stability studies for clients to determine the stability of their protein formulations. Additionally, we are well-versed in designing studies to compare the immunogenicity of various formulations as a function of aggregate content.

MICROBIAL EXPRESSIONBaroFold can conduct cytoplasmic expression of any client’s protein of interest, clone genes of interest in different expression vectors, and optimize expression.