cell concentration calculation - (Mar/12/2007 )

I need help with a cell culture calculation please: I have a cell concentration of 800000cells per mL in a total volume of 30mLs cell suspension. I would like this stock to be 9000000cells per ml for downstream injections. Can I pellet the original cell suspension and resuspend in an appropriate volume??

Ultimately, I require 300000cells per 100ul (or per mouse) and I have 30 mice to inject. Is it possible with the original amount?

I hope this makes sense to someone. I am pretty stuck.

Thank you,Sybr

-sybr-

hai you need to take 375 micro l from the original suspension to have 300000 cells.

-bioenthu-

QUOTE (sybr @ Mar 12 2007, 03:20 PM)

I need help with a cell culture calculation please: I have a cell concentration of 800000cells per mL in a total volume of 30mLs cell suspension. I would like this stock to be 9000000cells per ml for downstream injections. Can I pellet the original cell suspension and resuspend in an appropriate volume??

Ultimately, I require 300000cells per 100ul (or per mouse) and I have 30 mice to inject. Is it possible with the original amount?

I hope this makes sense to someone. I am pretty stuck.

Thank you,Sybr

for the first part. Centrif at 1500RPM and collect you cell pellet sediment. Here in sediment - 30*800000 = 24000000 cells. And dilute you sed with 2,66 ml appropriate medium for inj.

for the second part of your Q: for 30 mice you need total cell amount 90000000. You need to disolve your previous sediment in 8 ml media for inj. So you conc will be 300000 per 100 mkl. So you can inject with this volume 80 mice!