The present article addresses the fishing activity in the native indian Community in Gamboa-Peru through a spatial analysis of the territory; from the understanding of the local knowledge, the representativeness in both sectors economic, food, and cultural reproduction; it is widely developed by the inhabitants of the region as an integral part of their traditional knoledge. Undoubtedly, the pressure on natural resources is manifested in dissimilar processes of mineral exploitation, animal extraction, and flora, loss of strategic ecosystems as well as the gradual population growth and urban expansion experienced by surrounding cities to indigenous settlement. This context of struggle for the use and land tenure, requires the consideration regarding food sovereignty of indigenous communities, especially in the particularity of cross-border relations in the Triple Amazon frontier where Ticunas develop their ancient arts.