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Biological Strategies: Western Blot: p62/SQSTM1 Antibody [NBP1-48320] - Cultured HeLa cells were treated with or without 50 uM chloriquine for 24 hours as indicated. Cell lysates were prepared and separated on a ...read more

Simple Western: p62/SQSTM1 Antibody [NBP1-48320] - Lane view shows a specific band for p62/SQSTM1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Background

p62/SQSTM1 (sequestosome 1) is ubiquitously-expressed cytoplasmic/adapter protein conserved in vertebrates and it can be induced by proteasomal inhibitor PSI, PGJ2/prostaglandin J2 as well as phorbol 12-myristate 13-acetate (PMA). SQSTM1 has the ability of ubiquitin binding as well as regulating NFkB1 activation by TNF-alpha, NGF (nerve growth factor) and interleukin-1. SQSTM1 acts as an adapter that mediates the interaction between TRAF6 and CYLD, and SQSTM1-TRAF6 interaction leads to K63-linked polyubiquitination of TRAF6 followed by subsequent activation of NFkB pathway. SQSTM1 plays a role in TITIN/TTN downstream signaling in muscle cells and regulate signaling cascades through ubiquitination. SQSTM1 is essential both for formation and autophagic degradation of polyubiquitin-containing bodies which are known as aggresome-like induced structures (ALIS) and SQSTM1 connects ALIS to the autophagic machinery via direct interaction with MAP1 LC3 family members. Moreover, while autophagy modulates SQSTM1 protein levels, SQSTM1 suppresses autophagy via activation of mTORC1. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress. SQSTM1 functions as a signaling hub for various signal transduction pathways, apoptosis, cell differentiation, apoptosis, immune response, regulation of K+ channels and Nrf2 activation, and its dysregulation is associated with Paget disease of bone and tumorigenesis.

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This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for p62/SQSTM1 Antibody (NBP1-48320). (Showing 1 - 1 of 1 FAQs).

This technical query is related to Cat. No.: NBP1-48320. Technical Concern: As per the technical data sheet, p62/ SQSTM1 band is seen at ~47 kDa. We expect the band to be seen at 62 KDa for the domain p62/ SQSTM1, however only a ~47kDa band is visible in our blot, is there any explanation for this observation?

Regarding your concern with the performance of our p62 antibody (NBP1-48320), it is expected to see multiple bands depending on what form of p62 is present in the testing sample. According to uniprot, p62 has two different isoforms at 47kDa and 38kDa, however due to the interaction of p62 with other proteins and some possible modifications, a 62kDa band is usually observed on the blot. There is an abundance of supporting literature suggesting this antibody is performing well. p62 is considered to be the marker for Autophagy and the protein undergoes massive post translational modifications which can explain size increase. There are 13 phosphorylation sites on this protein. Here is the uniprot link for p62 (Q13501 reference 30); (PubMed 15592455).

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