XpressRef Universal Total RNA

XpressRef Universal Total RNA samples contain the broadest possible representation of genes from a specific organism (available for human, mouse, and rat). These RNA samples can be used in the troubleshooting of real-time RT-PCR applications and for internal normalization of microarray systems.

The XpressRef Universal Total RNA is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Principle

XpressRef Universal Total RNA samples contain the broadest possible representation of genes from a specific organism (available for human, mouse, and rat). RNA is prepared from various human, mouse, and rat organs to ensure the broadest gene coverage. RNA is prepared on an industrial scale using a modified guanidine isothiocyanate technique, which ensures high-quality RNA.

Human: Prepared from 20 different human adult and fetal normal major organs

XpressRef Universal Total RNA samples are useful in troubleshooting real-time or semi-quantitative RT-PCR gene expression analyses, when the gene of interest is difficult to detect, or to optimize real-time RT-PCR gene expression assays by allowing the determination of amplification efficiencies when experimental RNA samples are precious.

These RNA samples may also be used as an internal normalization control for two-color microarray systems or as a troubleshooting tool for single-color microarray systems.

Applications

XpressRef Universal Total RNA is highly suited for determination of real-time PCR amplification efficiencies, and standardization and troubleshooting of semi-quantitative and real-time RT-PCR. XpressRef Universal Total RNA is also highly suited for normalization control for two-color microarray systems and standardization and troubleshooting for single-color microarray systems