The easiest way to measure cell death is by measuring lactate dehydrogenase (LDH), a stable cytoplasmic enzyme which is present in all cells but only released when the plasma membrane is damaged.
The LDH Cytotoxicity Detection Kit provides a simple and precise colorimetric assay for LDH activity: a two-step enzymatic reaction creates a formazan dye that is easily measured by A492.

Why Use the LDH Cytotoxicity Detection Kit?

Sensitive assay: Detect as few as 2,000 dead or damaged cells per well.

Accurate results: The amount of formazan dye you measure is directly proportional to the number of dead or damaged cells.

Cost-effective: Get your LDH assay for a better price.

Supplier

Price

# of Rxns

Cost per rxn

Clontech

$326.00

2,000

$0.16

Competitor A

$410.00

500

$0.82

Competitor BP

$225.00

500

$0.45

Competitor BV

$245.00

500

$0.49

Competitor PK

$299.00

500

$0.60

Competitor SC

$175.00

500

$0.35

Cell-mediated cytotoxicty was measured with the LDH Cytotoxicity Detection Kit. Mouse spleen cells were stimulated in vitro and cytotoxic T lymphocytes (CTLs) were isolated. 10,000 test cells/well were incubated with the effector CTLs, and then cell death was measured using the LDH assay.

Fast, Simple LDH Assay Protocol

The LDH assay does not require prelabeling or washing steps. The entire procedure can be performed in a single 96-well plate.