AIF AntibodyCATALOG NUMBER: 2301

Western blot analysis of AIF in K562 with AIF antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.

Immunocytochemistry of AIF in K562 cells with AIF antibody at 5 μg/mL.

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0.02 mg - $79.00

0.1 mg - $325.00

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Additional Info

Background

Specifications

SPECIES REACTIVITY:

Human, Mouse, Rat

TESTED APPLICATIONS:

ELISA, ICC, WB

APPLICATIONS:

AIF antibody can be used for detection of AIF by Western blot at 0.5 - 2 μg/mL. A 67 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.

USER NOTE:

Optimal dilutions for each application to be determined by the researcher.

POSITIVE CONTROL:

1) Cat. No. 1204 - K562 Cell Lysate

2) Cat. No. 1404 - Mouse Liver Tissue Lysate

3) Cat. No. 1464 - Rat Liver Tissue Lysate

4) Cat. No. 17-004 - K-562 Cell Slide

PREDICTED MOLECULAR WEIGHT:

67 kDa

IMMUNOGEN:

AIF antibody was raised against a 14 amino acid peptide near the carboxy terminus of human AIF.

The immunogen is located within the last 50 amino acids of AIF.

HOST SPECIES:

Rabbit

Properties

PURIFICATION:

AIF Antibody is affinity chromatography purified via peptide column.

PHYSICAL STATE:

Liquid

BUFFER:

AIF Antibody is supplied in PBS containing 0.02% sodium azide.

STORAGE CONDITIONS:

AIF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Background

BACKGROUND:

AIF Antibody: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed.