Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Working dilution samples should be discarded if not used within 12 hours.

1/1000. Detects a band of approximately 48 kDa. (for whole cell extract of the human cell line HeLa and at a dilution of 1/5000 for Xenopus whole egg extract).

靶标

功能

Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.

序列相似性

Belongs to the tubulin family.

翻译后修饰

Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.

ICC/IF image of ab11321 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11321, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.

Thank you for your enquiry.
Details of the immunogens used for each of the antisera that you are interested in are as follows:
I think that you may have mistyped the first antiserum as it is not directed against tubulin.
CDCP1 antibody (ab1...

Thank you for your enquiry regarding ab11321. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimburseme...