Evaluation of the Free Electrophoretic Mobility at an Ionic Strength of 01 mol L

For reasons of comparability, the free electrophoretic mobility obtained for a given set of experimental conditions may be corrected to an effective mobility at an ionic strength of 0.1 mol L"1. This can be achieved by substituting the relevant values into Abramson's equation:

Uo.i = (U>o.I(k>O.I xRs + 2.4)) x (kO.I XRs + 2.4)-1

where U0.1 and U>0.1 (m2 V-1 s_1) represent the free electrophoretic mobilities at an ionic strength of 0.1 (mol m~3) and > 0.1 (mol m~3) respectively; k>0.1 and k0.1 represent the reciprocal of the effective thickness of the ionic cloud at ionic strength of 0.1 (mol m~3) and > 0.1 (mol m~3) respectively and RS (m) is the Stokes radius of the protein. For the experimental conditions given earlier:

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