Does anyone have experience with transient transfection of primary
cultures of neurons? I am interested in several questions:
What types of cultures have you used?
What did you use to transfect (CaPO; DEAE; Liposomes)?
What promoter did you use as a control (normalization)
construct?
Any advice or direction would be appreciated.
Matthew Frosch
frosch at bics.bwh.harvard.edu