what is difference of E.coli grow on different media? - (Aug/22/2008 )

Hi, Everyone:I try to do some experiment on surface. At first, i use E.coli on the agar plate, then I heard that I should grow E.coli in LB broth, though I prepare solution of OD= 0.2. but both of E.coli gave different responce. Could someone tell me the difference of E.coli grown on these two different media? I am sure not kill the cell or break them during processingThanks

-nonesuch-

QUOTE (nonesuch @ Aug 22 2008, 09:02 AM)

Hi, Everyone:I try to do some experiment on surface. At first, i use E.coli on the agar plate, then I heard that I should grow E.coli in LB broth, though I prepare solution of OD= 0.2. but both of E.coli gave different responce. Could someone tell me the difference of E.coli grown on these two different media? I am sure not kill the cell or break them during processingThanks

What agar? LB?

Your differences might not be related to the composition of specific medium/agar, but rather to the conditions of growth.

-Pallas-

which two media are you comparing?

But yes, different concentrations of nutrient (amino acids), types of carbon sources, nitrogen sources, oxygen availability will change the both the characteristics of E coli. This includes growth, exogenous protein expression, plasmid yields, etcs..

-perneseblue-

We don't know what "response" this guy thinks he's observing.

-jorge1907-

Hi, Everyone:Sorry for discribing unclearly. I used normal agar plate to grow e. coli. and take some colonies to dissolve in buffer solution. My reagent showed very positive responce to the existance of E.coli. However, I try to grow E.coli in LB broth, centrifuge, wash to get clean E.coli, then prepare the same buffer solution. My reagnt show no responce to the existance of E.coli. I think maybe during the growth, something appears on the surface of colony. if I use second method to grow E.coli, nothing special to give positive responce. Does someone know what appears during growing on agar plate but not growth in LB broth.Thanks