In the last years degradation of aromatic compounds by white-rot fungi has been intensively studied. Several studies revealed the abilities of the white-rot fungus Pleurotus ostreatus to degrade a variety of polycyclic aromatic hydrocarbons in liquid culture as well as in a semi-natural substrate. In the present study, two isolates of P. ostreatus (P50, P421) and a sample of P. ostreatus collected from Chitila woods were evaluated for their ability to use crude-oil as a source of carbon. The P. ostreatus samples were inoculated in plates with crude oil (1ml) spread on the entire carbon free mineral salt medium (MSM) surface and in plates with pieces of filter paper soaked in crude oil and placed around the inoculum. Two weeks after, the culture media were completely covered by mycelium. These results indicated that both isolates of P. ostreatus as well as P. ostreatus originated from Chitila forest were able to utilize the crude oil as a source of carbon and energy in their metabolism.