BC007 – Gel purification of TRIM6 variant 2 PCR product

Hannah’s amplicon mentioned in BC006 was run on a gel and was analyzed for the properly sized band. Landing at about 1,500 BP, the band showed promising results, so it was excised from the gel with a scalpel and put through the gel purification process. 5 microliters of the gel purified product were mixed with 2 microliters of purple loading dye and ran on a gel to estimate the concentration. I, unintentionally, skipped a crucial step in the gel purification process. The missed step lead to no visible results when run on a gel. The PCR will be completed again.

Life and Biology

Nicknamed "LaB", Life and Biology is a project initiated by Dr. Joel Graff. Here I share biology content for both my students at Montana Tech and anyone else that is curious about the topic. Feel free to snoop around the site and offer me any feedback you may have using the "Contact" page.