Red 580 dUTP

Red 580 [5-ROX] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Red 580 dUTP can also be used for multicolor fluorescence labeling.

This labeled dUTP can be used with the Nick Translation DNA Labeling System 2.0 (Prod. No. ENZ-GEN111).

Product Specification

Alternative Name:

5-ROX dUTP

Quantity:

Sufficient for approximately 49 reactions, following the recommended protocol of Prod. No. ENZ-GEN111.

Concentration:

1mM

Formulation:

Liquid. Solution in water.

Absorbance maximum:

580 nm

Emission maximum:

603 nm

Extinction Coefficient:

75,000 M-1 cm-1 (580 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA])

Correction Factor (260 nm):

0.44

Correction Factor (280 nm):

0.40

Purity:

≥93% (HPLC)

Appearance:

Purple liquid.

Applications:

FISH

Shipping:

Shipped on Dry Ice

Long Term Storage:

-20°C

Handling:

Protect from light. Avoid freeze/thaw cycles.

Technical Info/Product Notes:

Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.

Fluorescence emission profiles of available fluorescent labeled dUTPs. The dye-dUTPs are designed to perform especially well in multi-color applications, such as in situ hybridization and microarray analysis.

Fluorescence emission profiles of available fluorescent labeled dUTPs. The dye-dUTPs are designed to perform especially well in multi-color applications, such as in situ hybridization and microarray analysis.