Molecular cloning: a laboratory manual, Volume 1

From inside the book

Results 1-3 of 100

Page 3-5Cosmid. Vectors. In their simplest form, cosmid vectors are modified plasmids that
carry a copy of the DNA sequences (cos sequences) required for packaging DNA
into bacteriophage A particles. Because cosmids carry an origin of replication (
usually the ColEl origin) and a drug resistance marker (usually ampT), cosmid
vectors can be introduced into E. coli by standard transformation procedures (see
Chapter 1) and propagated as plasmids. To clone in cosmids, segments of
foreign ...

Page 3-7In addition to the basic features discussed above that are common to all cosmid
vectors, a variety of additional modifications have been made either to alleviate
problems of the kind listed on pages 3.54-3.55 or to confer special advantages
on individual vectors. These modifications include: • The incorporation of two cos
sites into a single cosmid vector (Bates and Swift 1983; Poustka et al. 1984). As
discussed later, the presence of these two sites greatly simplifies directional
cloning in ...

Page 3-52Preparation of a Transducing Lusate of Packaged Cosmids Because circular cosmid molecules carried in bacterial cells contain a cos site, they can be
efficiently packaged into bacteriophage A particles. When cosmid-containing
bacteria are infected with bacteriophage A (or when a resident prophage is
induced), the cos site in the cosmids is cleaved by the bacteriophage A ter
function. The linearized cosmid DNA is then packaged into the heads of newly
formed bacteriophage A ...

LibraryThing Review

User Review - iayork - LibraryThing

the BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ...Read full review

LibraryThing Review

User Review - iayork - LibraryThing

the BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ...Read full review