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Nephelometry. Polystyrene particles are coated with streptolysin O and DNase B antigens. The serum specimens containing antibodies will complex with the antigens, forming aggregations that will scatter light. The intensity of the resulting scattered light in the nephelometer is dependent upon the antibody content in the specimen. Calculations are based on the comparison of the specimens to standards of known concentrations.(Pacifico L, Mancuso G, Properzi E, et al: Comparison of nephelometric and hemolytic techniques for determination of antistreptolysin O antibodies. Am J Clin Pathol 1995 April;103[4]:396-399)