The QIAamp MinElute Media Kit uses well-established technology for purification of nucleic acids. The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 µl. The kit is suitable for use with liquid media containing nucleic acids, such as cervical swab transport media (e.g., PreservCyt or SurePath solution). Nucleic acids are eluted in Buffer AVE, ready for use in amplification reactions or storage. Purified nucleic acids are free of proteins, nucleases, and other impurities.

Procedure

The QIAamp MinElute Media procedure comprises 4 steps (lyse, bind, wash, elute) and is carried out using QIAamp MinElute columns on QIAvac 24 Plus vacuum manifolds. The procedure is designed to ensure that there is no detectable sample-to-sample cross-contamination, and allows safe handling of potentially infectious samples. The simple QIAamp MinElute procedure, which is highly suited for simultaneous processing of multiple samples, yields pure nucleic acid from 24 samples in less than 90 minutes.

Applications

The QIAamp MinElute Media Kit can be used for isolation of DNA from liquid media, such as cervical swab transport media. The kit can be used for purification of cellular, bacterial, and viral nucleic acids from a variety of sources, including: