Genetic Alterations:
BAC engineering was used
to insert an intron containing cre cassettes, followed by a polyadenylation sequence
to terminate transcription of the fusion transcript immediately after the recombinase
gene, into the BAC vector at the initiating ATG codon in the first coding exon
of the gene. The BAC address for this line is RP24-335M24. SNP analysis has shown
the transgenic insertion to be on chromosome 19 between SNPs UT_19_10.709331
and rs3655407.

This congenic strain carries
the same mutation as MMRRC:030071.
The mutant allele in this strain was originally characterized on a STOCK background
before being crossed to C57BL/6 for at least 8 generations. It should be noted
that the phenotype could vary from that originally described. BAC-transgenic
images of the central nervous system at various ages of the congenic mouse
can be found on the GENSAT
project page.

Strain of Origin: FVB/N

Strain genetic background: B6.FVB(Cg)

Strain Development: The original transgenic mice were created in the GENSAT project in collaboration with the Intramural Program of the National Institute of Mental Health. The transgenic mice have a modified BAC-cre injected into pronuclei of FVB/N fertilized oocytes. Offspring of founders were crossed to a Soriano Rosa26/stop/EGFP reporter line. The MMRRC backcrossed this transgene from the mixed background to C57BL/6J, selected for mice with only the cre expression, and eliminated the Rosa26-GFP from the line. Mice were determined to be congenic on C57BL/6J by SNP analysis.

Suggested Control Mice: Wild-type littermates or C57BL/6J

Research Applications

Cell Biology

Developmental Biology

Neurobiology

Research Tools

Strain Origin

Donor:
MMRRC facility at University of California, Davis

Primary Reference:

Colony and Husbandry Information

Special Considerations

In general, GENSAT BAC transgenic mouse lines should be utilized in the hemizygous state. Breeding of the BAC transgene to a homozygous state carries an increased risk of generating an unwanted phenotype for two reasons. First, the transgene inserts at random into the genome. Breeding the transgene to homozygosity results in mice that carry this insertion on both chromosomes, increasing the probability that the insertion will result in an observable phenotype. Second, BAC transgenes may carry passenger genes that are also expressed from the BAC vector. Breeding to homozygosity doubles the number of these genes carried in the transgenic mouse, increasing the possibility that a phenotype will result as a consequence of increased gene dosage. In our experience, observable phenotypes are rare in BAC transgenic mice when carried in the hemizygous state.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email
mmrrc@ucdavis.edu.

Appearance

Coat color: Black

Other:

Breeding

MMRRC Breeding System: Backcross or Sib-mating

Breeding Scheme(s):

Hemizygous Tg male x Wild-type female sibling or reciprocal mating

Hemizygous Tg male x C57BL/6J female or reciprocal mating

Generation: N8. Strain was determined to be congenic on C57BL/6J by SNP analysis.

Overall Breeding Performance: Good

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.

Viability and Fertility:

Female

Male

Homozygotes are viable:

Unknown

Unknown

Homozygotes are fertile:

Unknown

Unknown

Hetero/Hemizygotes are fertile:

Yes

Yes

Age Reproductive Decline:

Unknown

Unknown

Average litter size: 8

Recommended wean age: 3-4 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU).
A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded
to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain.
(Use the MMRRC Catalog Search to request more than one strain.)

MMRRC Item # - Description

DistributionFee/unit (US $)

Units

Notes

032109-UCD-RESUS

Litter recovered from cryo-archive

$2,022.00

Non-Profit

Litter

Recovered litter1; additional fees for any special requests.

032109-UCD-SPERM

Cryo-preserved spermatozoa

$437.00

Non-Profit

Aliquot

Approximate quantity.2

032109-UCD-EMBRYO

Cryo-preserved embryos

$1,038.00

Non-Profit

Aliquot

Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the
expense of resuscitating mice from the cryo-archive; you will
receive the resulting litter. The litter will contain at minimum
one mutant carrier; the actual number of animals and the gender
and genotype ratios will vary. (Typically, multiple breeder pairs
can be established from the recovered litter.) Prior to shipment,
the MMRRC will provide information about the animals recovered. If
you anticipate or find that you need to request specific
genotypes, genders or quantities of mice in excess of what is
likely from a resuscitated litter, you may discuss available
options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with
sufficient sperm to recover at least one litter of mice, as per
provided protocols, when performed at the MMRRC facility. The
MMRRC makes no guarantee concerning the success of these
procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient
number of embryos (in one or more vials and based on the transfer
success rate of the MMRRC facility) to transfer to at least two
recipients. The MMRRC makes no guarantee concerning embryo
transfer success experienced in the recipient investigator's
laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the
Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the
Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

The MMRRC is a collaborative effort, funded by grants from DPCPSI of the
NIH.