alpha-internexin is a ~66kD Class IV intermediate filament originally discovered as it copurifies with other neurofilament subunits. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M anbd NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.

Catalog #

136913

Applications

Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilution

Immunofluorescence: 1:500-1-1:000

Western Blot: 1:10,000-1:20,000 (brain or spinal cord homogenate; look for a major band at SDS-PAGE apparent MW of 66-68kD, depending on the species. The human protein runs at ~68kD, while rat, mouse and other species run at ~66kD. Minor bands at ~150kD are probably covalent dimers, and bands at ~50kD represent a-internexin breakdown products.)

Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Clone Type

Polyclonal

Isotype

IgG

Host

Rabbit

Concentration

Not determined

Form

Supplied as a liquid.

Purity

Serum

Immunogen

Recombinant corresponding to alpha-internexin.

Specificity

Recognizes NF66. Species Crossreactivity: human, mouse and rat.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.