Exposure of Clostridium acetobutylicum ATCC 824 to different lignocelluloses-related compounds was investigated in this study. It was found that several of the compounds, when present at low concentrations (0.25 g/l) in Reinforced Clostridial Media (RCM) supplemented with 4g/L Na-butyrate instead of Na-acetate, led to an overall enhanced metabolic activity from the cultures as demonstrated by an increased production of butanol, higher glucose consumption level and higher optical density achieved. With higher concentrations, for most of the compounds tested, the toxicity of the compounds led to significantly lower activities and even complete loss of viability. Identical tests performed with another acetone-butanol-ethanol producing strain, C. beijerinckii, showed this increased activity to be characteristic of only C. acetobutylicum.