PE and FITC - (Mar/07/2014 )

I am new to using FACS and now I am confused if I can use FITC and PE together as a muticolor application? I read somewhere that these channels have spillover into each others signals and might give misreadings. Could someone help me answer this question.

Thanks.

-kundanitesh-

This is correct, PE and FITC can spill over and you need to do compensation. Which FACS machine/software are you working with ?
As an alternative I could recommend you using FITC and Alexa647, if you can choose your colours, they usually work without compensation