Where sanitation is done by using the disinfectants, two are
more disinfectants should be used. Disinfectants may be ineffective against some
of the organisms called resistant organisms. A procedure should be developed to
identify such organisms and any effective disinfectant should be employed for
such organisms.

Sanitation procedure and the disinfectant used should be
validated. If any equipment is sanitized with the disinfectant, a cleaning validation should be done for the removal of the disinfectant. Residues of the
disinfectant should be removed properly from the equipment after cleaning.

Class A and class B area of sterile manufacturing should be
sanitized with the sterile disinfectants and detergents. Generally disinfectants
are sterilized by filtration using 0.2 µ membrane filters in sterile
conditions. Hold time period of diluted disinfectant should be validated.

Different types of disinfectants should be used in the
rotation. Disinfectants should include the sporicidal because most of the
disinfectants do not have sporicidal activity and effectiveness of these
disinfectants must be validated.

Fumigation of the sterile area is a useful way to sanitize
the air of sterile area. It can reduce the microbial contamination in the air. Time
of the fumigation can be validated for better results of fumigation. Area should
be de-fumigated properly before use. Hydrogen peroxide solution with silver
chloride is used in fogger for fumigate the controlled areas. It is available in
market with different brand names.

Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips. Email: .moc.enilediugamrahp@ofni