Mikael,
easiest probaly is to transfect a mutated construct that is not cleaved but
has chemically similar amino acids at the modified cleavage site. (unless
you have a MALDI-TOF at your hands where you could analyze the purified
band of question)
Wo
What would be the
>easiest way to check if it has really been cleaved or not? So far I haven't
>been able to find an antibody, or a peptidase to use, and with the larger
>extracellular constructs the size difference is a bit small to be reliably
>checked in SDS-PAGE.
Wolfgang Schechinger
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