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Topic: AFB spores (Read 6098 times)

ummm, i really can't find a picture of AFB spores under a microscope, now...if anyone know of one, i'd be happy if you would post a link, or send me that picture, also, information about magnification of that picture is important.

oh, i don't need them, he-hesomeone asked a few questions about micro scoping and that he'd like to try it, and maybe test for AFB, so....I'm trying to help, to find a good picture of AFB spores through microscope.

oh, i don't need them, he-hesomeone asked a few questions about micro scoping and that he'd like to try it, and maybe test for AFB, so....I'm trying to help, to find a good picture of AFB spores through microscope.

AFB cannot be found by microscope. It is found wih laboratorium cultivation and by that, it stands 130C temperature.

i'm not sure if we understand each other.around here, anyone willing and in doubt, can get his honey tested for AFB presency.so...many say AFB is always present so there are no negative results (although, if number of spores per some ammount does not exceed certain value, they declare that sample as negative)

so...what do they do with that honey? i mean...i can't imagine any other way than to look at the honey under a microscope.

oh, i don't need them, he-hesomeone asked a few questions about micro scoping and that he'd like to try it, and maybe test for AFB, so....I'm trying to help, to find a good picture of AFB spores through microscope.

AFB cannot be found by microscope. It is found wih laboratorium cultivation and by that, it stands 130C temperature.

I don't understand your statement.Why can't you see AFB spores with a microscope?

When collecting mushrooms, the only way to definitely know a safe mushroom from a poison/toxic look-a-like is to look at the spores.My books say that 400 power is sufficient.

Finksy, yes the spores are cultivated, but one has to use a microscope to see them, they are not visible by the naked eye, or am I missing something too. Have a wonderful and beautiful day. Cindi

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There are strange things done in the midnight sun by the men who moil for gold. The Arctic trails have their secret tales that would make your blood run cold. The Northern Lights have seen queer sights, but the queerest they ever did see, what the night on the marge of Lake Lebarge, I cremated Sam McGee. Robert Service

Finsky, I am standing corrected. The paper indicates that the paeninbacillus larvae larvae colonies are visible in the petri dish as white and have a concave form and rough surface. I think I misunderstood something.

So AFB colonies are visible, but is a microscope required to see the spores? I didn't read in that paper anything about that part.

There is another test called the Holtz milk test that can be performed as well to test for AFB. Can't recall an awful lot about this specific test, but it is a viable one for a quick ident on AFB. Beautiful day, great life. Cindi

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There are strange things done in the midnight sun by the men who moil for gold. The Arctic trails have their secret tales that would make your blood run cold. The Northern Lights have seen queer sights, but the queerest they ever did see, what the night on the marge of Lake Lebarge, I cremated Sam McGee. Robert Service

AFB is caused by Paenibacillus larvae , a spore-forming bacterium.A microscope slide can be prepared by dissolving a small part of an AFB scale. Stir the scale with a toothpick in a droplet of water placed on a slide and apply a cover slip.Under 400X magnification, the AFB spores are readily visible. AFB spores are characterized by being very slightly oblong, uniform in size and shape. The spores â€œjiggleâ€ in a characteristic Brownian movement.P. larvae is competitive and does not tolerate growth of other bacteria in the parasitized bee larva. As a result, most microscopic slides will show a predominance of P. larvae spores. This is not always the case with poor samples or those left in the collection bag for too long. In such case, secondary invaders such as moulds, will appear.Control and ;) RDY-B

It seemed to me that the discussion was leaning towards the cultivation of the spores.Spores do not make new spores.The cultivation of bacterium on the spores can lead to identification of spores because you can see a bacteria colony with the naked eye when you can't see the spores, hence, no microscope needed.

I was not aware that the bacteria was a spore forming bacteria, so, technically speaking, I can understand the term 'cultivation of spores' even though it is the 'cultivation of bacteria' that are producing the spores.

I apologize if I have caused any confusion and thanks for clearing this up for me.I should have done more reading before posting.