Part:BBa_R0065

cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR.

LuxI

cI

activity of promoter

+

+

zero

+

-

maximum

-

+

zero

-

-

leaky (no quantitative information)

Usage and Biology

Pretty good 'off' in the absence of LuxR/HSL. [jb, 5/24/04]

very leaky (no LuxR/HSL) at high (100-300) copy number. can only be slightly induced by LuxR+3OC6HSL. (jt, ut-austin; 08/11/05)

Part has since been improved by the 2010 MIT iGEM team in the form of the part Part:BBa_K415032 where the second -10 promoter region has been removed, and the part is thus less leaky.