Molecular Cloning Kits

The products in this section are designed for molecular cloning of cDNA, gene and PCR fragments (up to 15kb) and also the construction of libraries for much larger fragments (up to 40kb) such as fosmids. Kits are available with both electrocompetent and chemically competent cells. In regards to vector and insert preparation, products in this section enable recovery of nucleic acids from agarose gels, sheared DNA can be repaired and then ligated for blunt-end cloning, genomic DNA cloning and subcloning.

Applications

These molecular cloning products have high efficiency for DNA ligations and high throughput DNA cloning. Readily clone PCR or RT-PCR products and accomplish a wide range of DNA engineering tasks.

Product Highlights

CopyControl™ Fosmid Library Production Kits

Lucigen

CopyControl™ Fosmid Library Production Kits provide an efficient and improved method for constructing a library of cosmid-sized (approximately 40kb) clones. The CopyControl™ Fosmid Library Production Kit contains the original pCC1FOS™ Vector, while the recently introduced CopyControl™ HTP Fosmid Library Production Kit includes a modified pCC1FOS Vector, called pCC2FOS. Both of these CopyControl™ Vectors contain both the E. coli F-factor single-copy origin of replication and the inducible high-copy oriV. CopyControl™ Fosmid clones are grown at single copy to ensure insert stability and successful cloning of encoded and expressed toxic protein. The CopyControl™ Fosmid clones can then be induced up to 50 copies per cell.

Red/ET Recombination technology

Gene Bridges

Red/ET is a revolutionary method for DNA engineering. Recombineering with Red/ET allows unlimited cloning, subcloning, and modification of DNA at any chosen position. It permits precise engineering of DNA molecules of any size, including very large ones such as BACs or the E.coli chromosome.

Advantages over conventional methods

Independent of restriction sites

No size limits

No unwanted mutations

Rapid

Red/ET Recombineering is one simple and powerful tool to modify plasmids, BACs, and even E.coli chromosomes.

CopyCutter™ EPI400™ E.coli* cells

Lucigen

CopyCutter™ EPI400™ E.coli* cells were developed to significantly lower the copy number of a wide variety of common vectors so that you can more readily clone unstable DNA sequences. DNA that is unstable at high-copy number often codes for a protein that inhibits cell growth or contains AT- and GC-rich sequences or sequences with strong secondary structure. The CopyCutter™ EPI400™ cell line was derived from our high-transformation efficiency phage T1-resistant TransforMax™ EC100™ E. coli strain by manipulating a gene that controls the copy number of vectors containing ColE1 or pMB1 origins of replication (e.g., pUC- and pET-type vectors).

MaxPlax™ Lambda Packaging Extract

Lucigen

MaxPlax™ Lambda Packaging Extract offers maximum in vitro packaging efficiencies of cos site-containing methylated or unmethylated DNA. Traditional lambda packaging extracts are derived from two complementary lysogenic E. coli strains, BHB2690 and BHB2688, as described by Hohn. MaxPlax™ Extract utilises a restriction-free E. coli K-12 strain, NM759, in place of strain BHB2690. The sonicated extract of NM759 is combined with an extract of BHB2688 to produce MaxPlax™ Extract.