RecombinantHuman FZD8 protein was expressed in Chinese Hamster Ovary cell line with theFc part of Human IgG1 Pro100-Lys330 (C-terminus) and Human Frizzled-8 Ala25-Pro172(N-terminus).

Description:

FZD8 is one of atleast ten seventransmembrane (7TM) glycoprotein of the Frizzled family of Wntreceptors. Frizzled proteins are thought to be G-protein-coupled. Wnt engagement,with low density lipoprotein receptor related proteins LRP-5 or LRP-6 actingas co-receptors stabilizes β-catenin and promotes gene transcription that isimportant in development and tissue maintenance.

Source:

Chinese HamsterOvary cell line, CHO-derived

Form:

Supplied as a 0.2 μmfiltered solution in PBS.

N-terminal Sequence:

Ala25 & Ala28

Molecular Weight:

43.3 kDa

Activity:

Measured by itsbinding ability in a functional ELISA. In a 100 μL reaction mixturecontaining biotinylated rmWnt-3a or rmWnt-5a at 50-100 ng/mL and rhFrizzled8/Fc Chimera dilutions at 0.1-8,000 ng/mL, the concentration of rhFrizzled8/ FcChimera that produces 50% of the optimal binding response is found to beapproximately 7-28 ng/mL.

Endotoxin Level:

<1.0 EU per 1 μgof the protein by the LAL method.

SDS-PAGE:

55-65 kDa, reducingconditions

Purity:

>95%, by SDS-PAGEunder reducing conditions and visualized by silver stain.