RE: Drying artefact

From:

"Hall, John"

Stuart Lamonby
The drying artefact you describe may be similar to the one we experience. It
affects mainly connective tissues (cartilage, bone & collagen) and keratin
but may occur in any tissues. Thick cytopreps are particularly affected.
I believe this is the same artefact previously discussed on Histonet as
"cornflaking artefact".
From our experience do not waste your time (in our case many months)trying
to resolve the problem by looking at your reagents and procedures. The
coverslipper is unlikely to be the problem either. Bayer engineers in
Australia spent months looking for a mechanical cause without success.
The artefact can be eliminated by removing the film then re-coverslipping.
The problem is with the tape.
In our lab the problem appeared suddenly and could be traced back to the
delivery of a new batch of tape (November 2000).
We were told initially that the problem was restricted to our lab but as we
are a reference centre we were able to review slides from many other labs to
find that it is a common problem. I suspect many labs are not reviewing all
their slides and so are unaware of the extent of the problem. When
questioned, our Pathologists confirmed that they often see this artefact in
slides from many labs.
While we have no formal acknowledgement that the fault is with the tape,
Bayer have conceded that it is not a machine problem.
Bayer began supplying us with fresh batches of tape as they arrived in
Australia. Nothing changed and the artefact persists.
Bayer did provide us with one sample roll of tape from Sakura (?R&D)which
showed no artefact in any slides coverslipped but this was a one off and
they have been unable to supply us with this tape on an ongoing basis.
Tape from an alternative supplier (Klinipath in the Netherlands) also shows
this drying artefact and when I discussed this problem with their company
representatives they were aware of the artefact but considered it to be
inherent in tape coverslipping.
I am sure that Sakura and Bayer are aware of the problem but have not so far
resolved it, at least not to this customers satisfaction.
John Hall
Senior Scientist
Anatomical Pathology
Alfred Hospital
Commercial Road, Prahran, Victoria, 3181, Australia
Email: J.Hall@alfred.org.au
Phone: +613 9276 2767
Fax: +613 9276 2899
-----Original Message-----
From: HistoNet Server [mailto:histonet@pathology.swmed.edu]
Sent: Wednesday, July 24, 2002 3:45 PM
To: HistoNet Server
Subject: Daily Digest
----------------------------------------------------------------------
Date: 23 Jul 2002 02:43:32 -0500
From: Stephen.Eyres@sanofi-synthelabo.com
Subject: Re: Fluorogold
Hi Cheryl,
Are you interested in healthy or injured neurones? We are about to start
evaluating Fluoro Jade B for injured neurones. This works on paraffin
sections.
Steve
Gayle Callis
ana.edu> histonet@pathology.swmed.edu
cc:
22/07/2002 Subject: Re: Fluorogold
16:08
Hi Cheryl,
We put frozens on Plus charge for IFA technics, frozens and polysine should
work. There are also Plus gold that help with acetone fixed sections, bit
more expensive, get some samples from Erie Scientific, supposed to have
superior holding power, according to directions, acetone fixed but some
people use them for MOHS, and even aldehyde fixation. Not sure why Erie
says for acetone fixation?? worth a try.
You can also let the sections dry longer over 16 mesh silica gel, that may
help too - overnight.
At 07:43 AM 7/22/02 -0500, you wrote:
>Good morning Histonetters - I have a question from a graduate student and
>having never done this technqiue I am at a loss. The graduate is trying
>to do a fluorogold technique on ganglia. She is cutting frozen
>sections. The sections are terrible (I will help her there), but they
>consistently wash off.
> Question: Should the tissues be put on coated slides, Probe-On
>slides, charged slides, etc. to help the tissue stick or will that
>interfere with the fluorgold deposition?
> Any help you can offer will be greatly appreciated. Cheryl
>
>
>Cheryl Crowder, BA, HTL(ASCP)
>Chief Technologist
>Department of Pathobiological Sciences
>School of Veterinary Medicine
>Louisiana State University
>Baton Rouge, LA 70803
>
>(225) 578-9784
>FAX (225) 578-9720
>
>
>
>
>
>
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
email: gcallis@montana.edu
----------------------------------------------------------------------
Date: 23 Jul 2002 03:16:21 -0500
From: louise renton
Subject: DNA extraction
Dolores,
If the tissue is not embedded, but straight out of the fixative, wash the
chopped up sample for a couple of hours in several changes of sterile water
and then proceed as usual to the digestion step (spin down before each
change to remove as much of the water as possible).
Extraction of DNA for PCR from long stored formalin fixed, paraffin wax
embedded tissues can be a problem, as the DNA becomes degraded over time,
(I tried to get PCRable DNA from blocks embedded in 1942, with no joy) and
standard phenol/coloform extraction (ref Maniatis) might be better and give
larger yield than the Quigen kit.
Hope this helps
Louise Renton
>From: Dolors Fuster
>To: histonet@pathology.swmed.edu
>Subject: DNA
>Date: Mon, 22 Jul 2002 20:26:41 +0100
>
>Hi histonetters !
>
>What do you recommend us to obtain the best results in DNA extraction
>from formalin fixed tissue? should we do any kind of treatment before
>starting the
>extraction? (Water rinses, alcohol changes....)
>
>We are usin Qiagen kit with good results for parafin embeded samples
>fresh tissue and other kind of samples (cell culture, serum...). but
>are not successful with formalin fixed tissue
>
>Thanks in advance
>Dolors Fuster
>
>Tecnic Especialista en
>Anatomia Patologica
>Departament d'Anatomia i Embriologia Humana
>Facultat de Medicina (HCP)
>Universitat de Barcelona
>ICQ 14146798
>
>http://www.fashionmas.com/click/
Louise Renton
Bone Research Unit
MRC
Johannesburg
South Africa
Tel & fax +27 11 717 2298
"Time flies like an arrow, friut flies like a banana"
ex
_________________________________________________________________
Join the worldis largest e-mail service with MSN Hotmail.
http://www.hotmail.com
----------------------------------------------------------------------
Date: 23 Jul 2002 08:10:37 -0500
From: JHoffpa464@aol.com
Subject: Re: DNA extraction
oncor, used to make a parriffin DNA extraction kit. if they are still in
buisness.
----------------------------------------------------------------------
Date: 23 Jul 2002 08:22:15 -0500
From: "Vickroy, Jim"
Subject: Papers for endometrial curettings
When we are grossing endometrial curettings we have been using a tissue
paper to wrap the specimens and then place in a cassette.
Now everything sticks to the paper which is a real pain when at the
embedding center. The tissue paper we use is also used for hair
permanents. Does anybody have any ideas that work great?
Jim Vickroy
Memorial Medical Center
Springfield, IL
----------------------------------------------------------------------
Date: 23 Jul 2002 08:22:34 -0500
From: MTitford@aol.com
Subject: EM thick sections-again and again......
There have been several email messages on the Histonet recently about
billing
for electron microscopy thick sections. This was addressed recently in the
June 2002 edition of "CAP Today".
The posed question was" Our laboratory occasionally receives specimens for
electron microscopy. What code should we report if, after we prepare and
stain
thick sections, we determine electron microscopy cannot be performed?"
The answer was" Report the appropiate electron microscopy code (88348
electron
microscopy; diagnostic, or 88349, electron microscopy;scanning) with the -52
modifier, reduced services, if you have prepared and stained thick sections
and then you determine that electron microscopy cannot be performed. The
modifier indicates that part of the service was reduced while preserving the
identification of the basic service"
The College of American Pathologists is a pretty authoritive organization
and I would take their advice seriously.
Histotechnologists here in the States who work in the field of surgical
pathology, immunohistochemistry and EM would do well to read "CAP Today"
each
month. It is a free publication from the College of American Pathologists
and
gives good information relating to surgical pathology. It also occasionally
includes review articles about different subjects, for example,
immunohistochemistry surface markers, that while a little out of date, do
put
it all together in readable form that makes sense. They also give out little
practical tips that are of use.
Mike Titford
USA Pathology
Mobile AL USA
----------------------------------------------------------------------
Date: 23 Jul 2002 09:12:34 -0500
From: Stephen.Eyres@sanofi-synthelabo.com
Subject: Re: Papers for endometrial curettings
Hi Jim,
When I worked in Hospital pathology we used shiney toilet tissue. It worked
great.
Steve
"Vickroy,
Jim" To:
histonet@pathology.swmed.edu
Subject: Papers for endometrial
curettings
23/07/2002
13:44
When we are grossing endometrial curettings we have been using a tissue
paper to wrap the specimens and then place in a cassette.
Now everything sticks to the paper which is a real pain when at the
embedding center. The tissue paper we use is also used for hair
permanents. Does anybody have any ideas that work great?
Jim Vickroy
Memorial Medical Center
Springfield, IL
----------------------------------------------------------------------
Date: 23 Jul 2002 09:24:37 -0500
From: tec-lab-dep01@med.ub.es
Subject: DNA extraction for PCR
Thanks a lot Louise Renton for understanding my question with so
few clues.
Tissues come from an anencephal fetus that has been in formol for at
least 30 years. We do the DNA extraction for a PCR
I thought in washing the tissue before starting with qiagen protocol
but also thought it would be a good idea to ask it to somebody with more
experience.
I don't know exactly why we are having quite good results with
parafin sections and not the same with only fixed tissue....may be
because in parafin sections formalin rests have disappeared after
washing with water and diferent changes in alcohol?...I really don't
know, it's sure that somebody better than me in this field
can solve the doubt.
From: "louise renton"
To: TEC-LAB-DEP01@med.ub.es, histonet@pathology.swmed.edu
Subject: DNA extraction
Date: Tue, 23 Jul 2002 07:33:03 +0000
Dolores,
If the tissue is not embedded, but straight out of the fixative, wash the
chopped up sample for a couple of hours in several changes of sterile water
and then proceed as usual to the digestion step (spin down before each
change to remove as much of the water as possible).
Extraction of DNA for PCR from long stored formalin fixed, paraffin wax
embedded tissues can be a problem, as the DNA becomes degraded over time,
(I tried to get PCRable DNA from blocks embedded in 1942, with no joy) and
standard phenol/coloform extraction (ref Maniatis) might be better and give
larger yield than the Quigen kit.
Hope this helps
Louise Renton
>From: Dolors Fuster
>To: histonet@pathology.swmed.edu
>Subject: DNA
>Date: Mon, 22 Jul 2002 20:26:41 +0100
>
>Hi histonetters !
>
>What do you recommend us to obtain the best results in DNA extraction
>from formalin fixed tissue? should we do any kind of treatment before
>starting the
>extraction? (Water rinses, alcohol changes....)
>
>We are usin Qiagen kit with good results for parafin embeded samples
>fresh tissue and other kind of samples (cell culture, serum...). but
>are not successful with formalin fixed tissue
>
>Thanks in advance
>Dolors Fuster
>
>Tecnic Especialista en
>Anatomia Patologica
>Departament d'Anatomia i Embriologia Humana
>Facultat de Medicina (HCP)
>Universitat de Barcelona
>ICQ 14146798
>
>http://www.fashionmas.com/click/
Louise Renton
Bone Research Unit
MRC
Johannesburg
South Africa
Tel & fax +27 11 717 2298
"Time flies like an arrow, friut flies like a banana"
ex
_________________________________________________________________
Join the worldis largest e-mail service with MSN Hotmail.
http://www.hotmail.com
Dolors Fuster
Tecnic Especialista en
Anatomia Patologica
Departament d'Anatomia i Embriologia Humana
Facultat de Medicina (HCP)
Universitat de Barcelona
ICQ 14146798
http://www.fashionmas.com/click/
----------------------------------------------------------------------
Date: 23 Jul 2002 09:25:02 -0500
From: Andrew.Shand@north-bristol.swest.nhs.uk
Subject: RE: Papers for endometrial curettings
You might try Shandon's biopsy bags. We pour small specimens into a bag
through a wide-mouth "particle funnel". Bigger pieces may be placed in the
bag. Easy to see the specimen and tear open the bag when embedding.
Andy Shand
- -----Original Message-----
From: Vickroy, Jim [mailto:Vickroy.Jim@mhsil.com]
Sent: 23 July 2002 13:44
To: histonet@pathology.swmed.edu
Subject: Papers for endometrial curettings
When we are grossing endometrial curettings we have been using a tissue
paper to wrap the specimens and then place in a cassette.
Now everything sticks to the paper which is a real pain when at the
embedding center. The tissue paper we use is also used for hair
permanents. Does anybody have any ideas that work great?
Jim Vickroy
Memorial Medical Center
Springfield, IL
----------------------------------------------------------------------
Date: 23 Jul 2002 09:43:15 -0500
From: Vivian English
Subject: pcna/gma
Does anyone know where I can find a procedure for doing PCNA on GMA sections
of fish gonads? Please forward if possible. Thanks for this and all the
valuable info I!
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Does anyone know where I can find a procedure for doing PCNA on GMA
sections
of fish gonads? Please forward if possible. Thanks for this and all the
valuable info I!

- --Boundary_(ID_QgHqiBaAeH2t7/xEor5J4g)--
----------------------------------------------------------------------
Date: 23 Jul 2002 09:57:53 -0500
From: Walzer Susan
Subject: RE: Papers for endometrial curettings
The paper needs to be wet. (preferrably with formalin) Another way to be
sure bloody tissue does not stick to the paper is to fix it first, at least
for a few minutes in formalin. We use Obex round papers for everything.
Susan L. Walzer
- -----Original Message-----
From: Vickroy, Jim [mailto:Vickroy.Jim@mhsil.com]
Sent: Tuesday, July 23, 2002 8:44 AM
To: histonet@pathology.swmed.edu
Subject: Papers for endometrial curettings
When we are grossing endometrial curettings we have been using a tissue
paper to wrap the specimens and then place in a cassette.
Now everything sticks to the paper which is a real pain when at the
embedding center. The tissue paper we use is also used for hair
permanents. Does anybody have any ideas that work great?
Jim Vickroy
Memorial Medical Center
Springfield, IL
----------------------------------------------------------------------
Date: 23 Jul 2002 09:58:09 -0500
From: Walzer Susan
Subject: RE: Fite control
I had a pathologist bring me a block years ago from Carvelle( sp.?) in
Lousiana.(I think it was a hospital for Hansen's) I do not know if it is
still there. I heard that armadillos are the only other animal to carry this
disease. Would love to know more..
Susan L. Walzer
- -----Original Message-----
From: Johnston, Kathy [mailto:Kathy.Johnston@CLS.ab.ca]
Sent: Monday, July 22, 2002 2:20 PM
To: Histonet (E-mail)
Subject: Fite control
Hi Histonetters!
My current problem, is my search for some new Fite control blocks. Leprosy
is apparently not rampant on the prairies (Canadian that is)!
Does anyone know where I can get some new blocks/tissue to process?? Am
willing to beg, trade, etc!! Might have to go to Molokai and self biopsy
soon if I don't find any!
Any help would be greatly appreciated!
Kathy Johnston
Tech II - Special Stains
Anatomic Pathology - Foothills Medical Center
Calgary Laboratory Services
Ph - 403-944-4760
Fax - 403-270-4093
kathy.johnston@cls.ab.ca
----------------------------------------------------------------------
Date: 23 Jul 2002 10:10:41 -0500
From: j.brook@talk21.com
Subject: nuclear meltdown/bubbling
Does anybody know of any research papers or documents about the nuclear
artefacts known as meltdown and bubbling. I am about to start an MSc project
investigating the cause of these artefacts and I am having trouble finding
references.
- --------------------
talk21 your FREE portable and private address on the net at
http://www.talk21.com
----------------------------------------------------------------------
Date: 23 Jul 2002 10:29:39 -0500
From: Terry.Marshall@rothgen.nhs.uk
Subject: RE: Papers for endometrial curettings
Agree this works well, though is somewhat resistant to folding.
In the UK, Izal and Bronco were 2 brands which were of this type (the
'scraping' rather than 'dabbing' type).
Not sure if they are even available now, even the men like 'soft' toilet
paper. Wimps!
Terry L Marshall B.A.(Law), M.B.Ch.B., F.R.C.Path
Consultant Histopathologist
Rotherham General Hospital, Yorkshire
terry.marshall@rgh-tr.trent.nhs.uk
- -----Original Message-----
From: Stephen.Eyres@sanofi-synthelabo.com
[mailto:Stephen.Eyres@sanofi-synthelabo.com]
Sent: 23 July 2002 14:44
To: Vickroy, Jim; histonet@pathology.swmed.edu
Subject: Re: Papers for endometrial curettings
Hi Jim,
When I worked in Hospital pathology we used shiney toilet tissue. It worked
great.
Steve
"Vickroy,
Jim" To:
histonet@pathology.swmed.edu
Subject: Papers for endometrial
curettings
23/07/2002
13:44
When we are grossing endometrial curettings we have been using a tissue
paper to wrap the specimens and then place in a cassette.
Now everything sticks to the paper which is a real pain when at the
embedding center. The tissue paper we use is also used for hair
permanents. Does anybody have any ideas that work great?
Jim Vickroy
Memorial Medical Center
Springfield, IL
----------------------------------------------------------------------
Date: 23 Jul 2002 10:53:17 -0500
From: Gayle Callis
Subject: Re: Papers for endometrial curettings
An old trick we use to keep fresh tissues from sticking is wet the papers
with fixative BEFORE putting currettings or tissues in contact with paper.
Also kept a flat metal weighing spatula to gently scrape (be careful not to
get paper fibers) currettings off paper which should be sitting on the hot
surface of embedding center. If cooled, will not release tissues.
At 07:44 AM 7/23/02 -0500, you wrote:
>When we are grossing endometrial curettings we have been using a tissue
>paper to wrap the specimens and then place in a cassette.
>Now everything sticks to the paper which is a real pain when at the
>embedding center. The tissue paper we use is also used for hair
>permanents. Does anybody have any ideas that work great?
>
>Jim Vickroy
>Memorial Medical Center
>Springfield, IL
>
>
>
>
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
email: gcallis@montana.edu
----------------------------------------------------------------------
Date: 23 Jul 2002 11:59:05 -0500
From: Gayle Callis
Subject: currettings, nylon bags, tissue papers
Endometrial currettings in nylon bags from Shandon (they have 3 or 4
sizes), other companies have these too. We have used stiff, smooth lens
paper (but make sure it is not going to shred as some papers are sturdier
than others). A fine mesh bag could be made from recycled ladies knee high
sheer nylon dress socks, pantyhose was suggested some years ago for large
bone slabs and did work - so everyone can laugh now! Or go to a fabric
store and buy some fine nylon mesh fabric, make own bags??
What about the mesh cassettes available these days, how do people like
them? There is one with an mesh insert that folds over to hold currettings,
looked like a nice design for this purpose.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
email: gcallis@montana.edu
----------------------------------------------------------------------
Date: 23 Jul 2002 11:59:20 -0500
From: "Dawson, Glen"
Subject: RE: Breast Core bx. IHC
All,
I was curious as to whether or not anyone else is experiencing a marked
difference in nonspecific IHC staining between breast core biopsies and
large pieces of breast. I am running into alot of nonspecific cytoplasmic
staining on my breast core biopsies that does not occur on the larger pieces
of breast from the same case. We have attempted different processing times
to prevent it but to no avail. The biggest difference between the handling
of the breast Bigs and the breast Bx's is the processing times. My logic is
that the core biopsy should not need the extended processing due to it's
small size. I'm at a loss. Any like experiences or suggestions would be
appreciated.
Thanx in Advance,
Glen Dawson BS, HT & IHC (ASCP)
Lead IHC Technologist
Milwaukee, WI
----------------------------------------------------------------------
Date: 23 Jul 2002 11:59:38 -0500
From: Robbin Newlin
Subject: fungus slide
Hi All
I would like to thank everyone who replied to my post about needing a fungus
slide for the HT exam. We have received some material. Again thanks to
everyone this is one of the best resources I have found.
Robbin
Robbin Newlin, HT,QIHC ASCP
Manager Histology Core Facility
The Burnham Institute
10901 North Torrey Pines Road
La Jolla, CA. 92037
(858) 646-3100 Ext. 3552
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Hi All

I would like to thank everyone who replied to
my
post about needing a fungus slide for the HT exam. We have received some
material. Again thanks to everyone this is one of the best resources I have
found.

- --Boundary_(ID_1u1dnYlDGKmRxX+ps9IsTQ)--
----------------------------------------------------------------------
Date: 23 Jul 2002 11:59:54 -0500
From: "Dawson, Glen"
Subject: RE: Papers for endometrial curettings
We use lens paper here.
Glen Dawson
----------------------------------------------------------------------
Date: 23 Jul 2002 12:29:08 -0500
From: =?UNKNOWN?Q?Agust=EDn?= Venzano
Subject: Drying artifact
Dear netters: We have no authomatic coverslippers in Argentine histolabs,
but we are having the hell of a problem with drying artifacts. We usually
attribute these problems to bad quality of the mounting media, to
insufficient volume of media, etc. But we could never prevent this artifact.
What are your suggestions on this subject?
Sincerely yours
Agustin Jose Venzano Halliburton
DVM-INTA Castelar, Argentina
----------------------------------------------------------------------
Date: 23 Jul 2002 12:46:18 -0500
From: "Histologyjobs.com"
Subject: fyi
Hello Histonetters,
Histologyjobs.com now runs all job postings until they
are filled. To post a job click on the following link:
http://histologyjobs.com/employer.htm
To search all current Histology and Cytology jobs click
on the following link:
http://histologyjobs.com/search.htm
Thanks,
Scott Glasgow
VP of Operations
LabSites
http://www.jobs4radiology.com
http://www.histologyjobs.com
http://www.jobs4medtechs.com
http://www.mynursejobs.com
http://www.jobs4pharmacy.com
http://www.jobs4rehab.com
----------------------------------------------------------------------
Date: 23 Jul 2002 12:46:48 -0500
From: "Martin, Ronald"
Subject: RE: currettings, nylon bags, tissue papers
Sakura makes mesh biopsy cassettes, multiple colors, 500/case for
$306.00/case. Fisher also makes biopsy bags. They may work well for
curretting cases.
Ron
-----Original Message-----
From: Gayle Callis [mailto:gcallis@montana.edu]
Sent: Tuesday, July 23, 2002 12:25 PM
To: histonet@pathology.swmed.edu
Subject: currettings, nylon bags, tissue papers
Endometrial currettings in nylon bags from Shandon (they
have 3 or 4
sizes), other companies have these too. We have used stiff,
smooth lens
paper (but make sure it is not going to shred as some papers
are sturdier
than others). A fine mesh bag could be made from recycled
ladies knee high
sheer nylon dress socks, pantyhose was suggested some years
ago for large
bone slabs and did work - so everyone can laugh now! Or go
to a fabric
store and buy some fine nylon mesh fabric, make own bags??
What about the mesh cassettes available these days, how do
people like
them? There is one with an mesh insert that folds over to
hold currettings,
looked like a nice design for this purpose.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
email: gcallis@montana.edu
----------------------------------------------------------------------
Date: 23 Jul 2002 13:06:50 -0500
From: rkline@emscience.com
Subject: Re: Drying artifact
I think it is in the tissue processing. What is your tissue processing
schedule? What tissue processor are you using?
=?UNKNOWN?Q?Agust=EDn?= Venzano on 07/23/2002
01:29:15 PM
To: Histonet Server
cc:
Subject: Drying artifact
Dear netters: We have no authomatic coverslippers in Argentine histolabs,
but we are having the hell of a problem with drying artifacts. We usually
attribute these problems to bad quality of the mounting media, to
insufficient volume of media, etc. But we could never prevent this
artifact.
What are your suggestions on this subject?
Sincerely yours
Agustin Jose Venzano Halliburton
DVM-INTA Castelar, Argentina
----------------------------------------------------------------------
Date: 23 Jul 2002 13:19:56 -0500
From: kbowden@ucsd.edu
Subject: Re: currettings, nylon bags, tissue papers
I use the mesh cassettes. They do save some time when your putting tissue
in
cassettes. When it is time to embed you have to look for the tissue so
marking the tissue in the alcohol would be useful.
Karen
Department of Orthpedics
University of CA, San Diego
9500 Gilman Dr.
La Jolla, CA
Gayle Callis wrote:
> Endometrial currettings in nylon bags from Shandon (they have 3 or 4
> sizes), other companies have these too. We have used stiff, smooth lens
> paper (but make sure it is not going to shred as some papers are sturdier
> than others). A fine mesh bag could be made from recycled ladies knee
high
> sheer nylon dress socks, pantyhose was suggested some years ago for large
> bone slabs and did work - so everyone can laugh now! Or go to a fabric
> store and buy some fine nylon mesh fabric, make own bags??
>
> What about the mesh cassettes available these days, how do people like
> them? There is one with an mesh insert that folds over to hold
currettings,
> looked like a nice design for this purpose.
>
>
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology - Marsh Lab
> Montana State University - Bozeman
> 19th and Lincoln St
> Bozeman MT 59717-3610
>
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> email: gcallis@montana.edu
----------------------------------------------------------------------
Date: 23 Jul 2002 13:31:10 -0500
From: Jill Songer
Subject: Re: Drying artifact
What type of artifact?
At 01:29 PM 7/23/2002 -0300, =?UNKNOWN?Q?Agust=EDn?= Venzano wrote:
>Dear netters: We have no authomatic coverslippers in Argentine histolabs,
>but we are having the hell of a problem with drying artifacts. We usually
>attribute these problems to bad quality of the mounting media, to
>insufficient volume of media, etc. But we could never prevent this
artifact.
>What are your suggestions on this subject?
>
>Sincerely yours
>
>Agustin Jose Venzano Halliburton
>DVM-INTA Castelar, Argentina
>
**************************************************************************
Jill Songer HT (ASCP)
Histopathology Lab
Virginia Maryland Regional College
of Veterinary Medicine
Veterinary Teaching Hospital
Blacksburg, VA 24061
----------------------------------------------------------------------
Date: 23 Jul 2002 19:15:48 -0500
From: Cathy Gorrie
Subject: Re: rat/mouse H&E staining
Ron,
We had some trouble staining our rat brain and spinal cord sections
(30um) with H&E (Usually we use cresyl violet). I am assuming you
are talking about frozen tissue sections. Our biggest problem was
patchy staining, and that the thicker sections were too dark. In the
end we used the standard H&E run used for parafin sections, Harris
Haematoxylin (regressive) and eosin but with reduced times (esp for
eosin) and careful differentiation in acid alcohol.
I can't help with exact times as they differ so much between
batches/types of H&E.
The other thing we were finding was that we got much more even
results if the air dried sections were taken down through xylene and
alcohol in exactly the same way you would for parafin sections. If
you are using parafin embedded sections there should be no difference
in the staining for rat/mouse tissue to what you might usually use in
the clinical settings.
Cheers, Cath
At 9:16 AM -0400 22/7/02, Martin, Ronald wrote:
>Fellow histotechs,
>
>If anyone is willing to share their H&E staining procedure for rat/mice
>tissue I would greatly appreciate it. I am staining brain, skin, sub
>cutaneous tumors, stomach and whole 1 day olds. I would like to know what
>type of stains (brands), times and techniques (progressive or regressive)
>some of you are using. Once again, this is for rat/mice tissue in a
research
>setting, not a clinical setting.
>Thanks,
>Ron Martin, B.Sc., HT/HTL (ASCP)
- --
- ---------------------------------------------------
Cathy Gorrie
Scientific Officer
Neural Injury Research Unit,
Department of Anatomy
School of Medical Sciences,
University of New South Wales
Sydney, N.S.W. 2052
Phone: 61-2-9385 2462
Fax : 61-2-9313 6252
e-mail: c.gorrie@unsw.edu.au
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