Hi
I am working on LOH in lung cancer and have to use paraffin embedded tissue
as the source material.The DNA is recovered from the tissue after standard
Haemotoxylin/eosin staining .PCR is performed on this material with no further
purification so as to minimise further degradation.The yields are obviously
small and therefore I can not estimate the conc. by running it on a gel and
i presume that such crudely prepared DNA would be useless for analysis on
a spec. Any ideas?
Thanks in advance
Stuart Pritchard
mmd280 at abdn.ac.uk