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AB Anti-angiogenic effect of EPI and control compounds in the aortic rings

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Day 3,5 Opening the shell Day 4 CAM’s development Day 8 Treatment of the CAM Day 11 Photos and labeling of the CAM Chicken chorioallantoic membrane ex ovo model Development of the CAMs ex ovo on plastic weight boats covered with square Petri dishes and placed into the incubator at 38°C and 80% of humidity.  Preservation of the CAM’s integrity  Statistically more reliable data

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Conclusions EPI is anti-angiogenic without being cytotoxic or apoptotic in vitro but also in relevant in vivo models of angiogenesis EPI induces Hyperacetylation of histone H3 in both HUVEC and aortic rings Parallel microarray analyses underscored a list of genes exclusively affected by the EPI (5µM) but not by other HDAC or DNMT inhibitors in the context of angiogenesis Epigenetic regulation of genes