Talk:20.109(F08): Mod 1 Day 3 Agarose gel electrophoresis

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T/R lab

Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised. Nice work!!

And here's the gel with your recovered fragments/backbones, ready for ligation. Thanks to Michelle for running and posting this.

Lane

Sample

Lane

Sample

1

Blue Frag

8

Red BKB

2

Blue BKB

9

Orange Frag

3

Green Frag

10

Orange BKB

4

Green BKB

11

Purple Frag

5

Pink Frag

12

Purple BKB

6

Pink BKB

13

Yellow Frag

7

Red Frag

14

Yellow BKB

W/F lab

Drum roll.....Here's the W/F gel of the recovered products.
Green & Pink, you had a faint fragment band, but it was definitely there. Blue's band was very faint if there at all. (I increased the exposure to saturation, and thought I could see a band) Be sure to comment on these data in your lab report as well as possible sources of error. Even if you had perfect data, discussing possible areas for error is encouraged.

Use this image for your homework, but we have more fragment DNA if you need it.

Lane

Sample

Lane

Sample

1

Blue Frag

8

Yellow BKB

2

Blue BKB

9

Purple Frag

3

Green Frag

10

Purple BKB

4

Green BKB

11

Ladder

5

Pink Frag

12

6

Pink BKB

13

7

Yellow Frag

14

For the Green and Blue Teams, I re-ran a gel of your DNA this morning to double check. These samples were run according to the protocol in your lab book. (5 ul DNA) Assume that the ladder band you are interested in is still 125 ng.
Since the intensities are different than we originally thought (my mistake), the ratio is off, but you ended up with viable colonies.