In this work, a new passive on-chip blood cell/plasma separator using self-assembly of microspheres as a physical filter and a smart on-chip dynamic blood cell/plasma separator based on a series of pressure pulses in microchannel have been proposed, developed, and successfully characterized for as a part of the disposable polymer lab-on-a-chip point-of-care testing (POCT) clinical diagnostics. A method for self-assembly crystallization of silica microspheres has been investigated for the application as a physical filter. Combination of the vertical deposition method using capillary forces on a hydrophillic substrate with plasma polymer surface modification is used for developing selectively deposited self-assembled microspheres over a specific site of a microchannel. The developed 3D self-assembled silica microspheres over the filter region of the microchannel with their uniform porosity and structure have been fully characterized for separating blood cell/plasma in microchannel. Secondly, a new pulse-assisted dynamic separation technique has been introduced and developed for the application of blood cell/plasma separation without physical filters. The dynamic separation has been attained from the differential forces exerted by applying high amplitude, short duration pressure pulses to the blood sample in microchannels. In addition, the motion of the particles in liquid suspension solution in microchannel experienced by both various forces on particles and pressure pulses on suspension solution has been simulated to validate the principle and to optimize performance by computational analysis and parametric study. The developed pulse-assisted dynamic separator has been successfully developed and characterized on blood cell/plasma from human whole blood sample in microchannel. An on-chip pressure actuator for transporting a fixed-volume of the biofluid sample into a desired site in a lab-on-a-chip or mTAS has been investigated and implemented. A new solid energetic material and its thermolysis by localized heat output from an infrared (IR) source is used for producing non-toxic and biologically inert nitrogen gas to deliver the fixed-volume of sample on demand. A solid energetic material has been explored and fabricated on the chip with simple and cost-effective method.