sup][12],[22] Under physiological-like condition, that is, in CO [sub]2 /HCO [sub]3[sup]− buffered Tyrode solution, Na [sup]+ -HCO [sub]3[sup]− -dependent transporter, that is, NBC, has been found to be another acid extruder to accompany NHE to achieve acid equivalent extrusion following intracellular acidosis in many different cell types.

Each semen sample was divided into nine aliquots of 200 ul each, three replicates for each of the two experimental and control group, one of the experimental groups was supplemented with 50ul vitamin-E (a-tocopherol) solution (1mM) in Ringer- tyrode as described by Verma and Kanwar (1999).

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