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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 2-5x100,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Passages: 276;
Karyotype: 2n = 46
This cell line was derived from normal human amnion and shown to be contaminated with HeLa cells. They are mainly used for virus studies (susceptible to poliovirus 1). HeLa marker chromosomes and type A G6PD have been detected. This cell line was found to have a HeLa profile by DNA fingerprinting.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (Species confirmation. G6PD type A)