Compounds Ia-Ih were hydrogenated with Pd-C to give IIa-IIh, and their hypoglycemic activity was evaluated with a glucose oxidase kit and insulin load test on normal mice.

The purified product was found to be biologically active and to reduce the food intake and body weight of mice during tests.

The effects of hematopoietic stem/progenitor cells (HSPCs) expanded in the two step coculture with human bone marrow mesenchymal stem cells (hMSCs) on the hematopoietic reconstruction of irradiated NOD/SCID mice were studied.

Sublethally-irradiated NOD/SCID mice were transplanted with ex vivo expanded HSPCs with the dose of 8.5 × 106 cells per mouse.

After transplantation, the dynamics of WBC in the transplanted mice was measured periodically, and the Alu sequence fragment special for human in the transplanted mice was inspected by PCR.

After transplantation with expanded HSPCs, the population of WBC in the transplanted mice increased in 12 d and reached the first peak in 25 d, then showed the second increasing of WBC in 45～55 d.

Expanded cells from the coculture scheme appeared to be favorable for the second increasing of WBC in the transplanted mice.

Moreover, the level of IL-13 mRNA and IL-9 mRNA expressed by DCs in asthmatic mice was significantly higher than those in the control groups (P>amp;lt;0.05).

TK-/gE-/GP5m+ and TK-/gE-/GP5+ expressing the authentic GP5 protein were inoculated into Balb/c mice to evaluate their immune responses.

The results indicated that the protecting neutralization antibodies (the 3/6 vaccinated mice obtained 1:16) and cell immune responses induced by TK-/gE-/GP5m+ against PRRSV were higher than that induced by TK-/gE-/GP5+.

The biological activity of 19peptide was determined by 3-[4,5-dimethylthiazol-2-y1]-2,5-diphenytetrazolium bromide (MTT) assay, cell growth curve, the effect of the ascitic fluid transfevent H22 hepatoma on mice and via histopathological slices.