This book constitutes the refereed proceedings of the 28th International Conference on Data Analytics and Management in Data Intensive Domains, DAMDID/RCDL 2016, held in Ershovo, Moscow, Russia, in October 2016.The 16 revised full papers presented together with one invited talk and two keynote papers were carefully reviewed and selected from 57 submissions. The papers are organized in topical sections on semantic modeling in data intensive domains; knowledge and learning management; text mining; data infrastructures in astrophysics; data analysis; research infrastructures; position paper.