While not his most successful arrangement, it is an atypical representation of the Virgin for its time, and must have been shocking to some contemporary viewers. The allegory, at its core, is simple. The Virgin with the aid of her son, whom she holds, tramples on a serpent, the emblem of evil or original sin. Saint Anne, whom the painting is intended to honor, is a wrinkled old grandmother, witnessing the event. Flimsy halos crown the upright; the snake recoils in anti-halos. Both Mary and Jesus are barefoot; Jesus is a fully naked uncircumcised child. All else is mainly shadow, and the figures gain monumentality in the light.

If this painting was meant to honor the grandmother of Christ, it is unclear how the ungracious depiction of her wrinkled visage in this painting would have been seen as reverent or iconic. Further shock must have accrued, as stated by Bellori, at the Virgin Mary’s revealing bodice. One could speculate that the parallel diagonals of Jesus’ phallus and leg suggest that both battle the snake, with one its metaphorical equal.