More Details about

Quantity

480 Tests

Sample Type

Serum

Description

For the quantitative determination of total bilirubin in human serum. Bilirubin is a metabolite of the heme portion of heme proteins, mainly hemoglobin. Bilirubin is excreted into the intestine and bile from the liver. Elevation of total serum bilirubin may occur due to (1) excessive hemolysis or destruction of the red blood cells (e.g. hemolytic disease of the newborn), (2) liver diseases (e.g. hepatitis and cirrhosis), (3) obstruction of the biliary tract (e.g.. gallstones). Bilirubin reacts with diazotized sulfanilic acid to produce azobilirubin, which has an absorbance maximum at 560 nm in the dimethyl sulfoxide (DMSO) solvent. The intensity of the color produced is directly proportional to the amount of total bilirubin concentration present in the sample.