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Notes: In this study, the expression of various adipokine genes linked to inflammation was examined during differentiation of pre-adipocytes to adipocytes in primary culture. The effect of TNFα on expression of these adipokines in differentiated human adipocytes was also investigated. qPCR was used to measure expression levels of the various adipokines, including NGF, during development and upon TNFα exposure. In addition, secretion of adipokines into the culture media was measured by ELISA, the NGF Emax® ImmunoAssay System being used to determine NGF levels. (3502)

Notes: In this study, implantation of human bone marrow stem/progenitor cells (MSCs) into the hippocampus of immunodeficient mice was found to stimulate proliferation, migration and differentiation of endogenous neural stem cells. Results of immunohistochemical analyses were confirmed by ELISA. The NGF Emax® Immunoassay System was used to determine NGF levels in isolated hippocampi at various intervals after implantation of the MSCs. (3500)

Notes: Patients with locally advanced squamous cervical carcinoma were monitored for chemotherapy-induced peripheral neurotoxicity (CIPN). Plasma drawn from the study participants was stored at –80°C prior to testing for nerve growth factor (NGF)levels using the NGF Emax® ImmunoAssay System. The authors note that a highly significant correlation existed between a decrease in circulating levels of NGF and an increase in the severity of CIPN. (3338)

Notes: The authors studied the effects of a chronic intermittent cold stress regime on sympathetic nerve activation and ovarian physiology. To analyze whether this effect on noradrenaline was preceded by activation of the neurotrophic factor system responsible for growth and survival of sympathetic neurons, they measured nerve growth factor in adult female Sprague-Dawley rats using the NGF Emax® ImmunoAssay System. (2772)

Notes: After inducing myocardial infarction in feral dogs, transcardiac NGF levels were measured in the serum from 0.5 hours to 1 month later. This difference in NGF concentration between the coronary sinus and aorta was measured in triplicate samples using the NGF Emax® ImmunoAssay System. (3336)

Notes: Cerebellar granule neurons were isolated from 8-day-old Wistar rats and were glucose deprived for 1 hour in Earl’s balanced salt solution. This solution was collected and assayed for NGF using the NGF Emax® ImmunoAssay System. (2809)

Notes: The levels of NGF in culture medium of numerous human pancreatic cancer cell lines was determined using Promega's NGF Emax® ImmunoAssay System. NGF-induced pancreatic cancer cell growth seemed to be dependent on the expression levels and the balance of the TrkA and p75 receptors. (2334)

Notes: Levels of nerve growth factor in untreated and IL-1beta hippocampal neuronal cultures were monitored during homeostasis, hypoxia and reoxygenation. Untreated cells exhibited a decrease in both the expression and release of NGF under mild hypoxic stress whereas IL-1beta treated cells exhibited an increase in both expression and release of NGF. NGF levels were quantitated using Promega's NGF Emax® ImmunoAssay System. (2332)

Notes: The NGF Emax® ImmunoAssay System (NGF ELISA) was used to quantitate NGF in the rat medial septium, vertical limb of the diagonal band, area CA1 of the hippocampus and dentate guyrus of the hippocampus. Some detail of the referenced extraction procedure is provided. (2222)

Neuroscience91, 1027-1034.
Expression of nerve growth factor in astrocytes of the hippocampal CA1 area following transient forebrain ischemia.1999

Gottlieb, M., and Matute, C.

Notes: The NGF EMAX® ImmunoAssay System (NGF ELISA) was used to assay for NGF protein levels in the CA1 region of the hippocampus from rats experiencing a transient ischemia and control animals. References and detail of the tissue preparation is provided. (1113)

Notes: The NGF Emax® ImmunoAssay System was used to determine the level of NGF protein in different portions of the rat brain. Excellent detail is provided for sample preparation. The rats were treated for one week prior to assay with either vehicle or dexamethasone. NGF levels were normalized to the amount of protein in the extracts. (0390)

Notes: The NGF Emax® ImmunoAssay System was used to measure NGF released into conditioned media of cultured rat pancreatic beta cells. The amount of NGF released increased with glucose treatment of the cells. The release was not inhibited by KCl but was inhibited by dibutryl cAMP. (0455)

Notes: The NGF Emax® ImmunoAssay System was used to determine the NGF content of mouse lavage and serum as well as the conditioned media of mononuclear cells. Allergic conditions showed increases in NGF. (1400)

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