JAMS 2018 TOAST Workshop

3rd generation sequencing technologies, also known as long-read sequencing, is continuing to revolutionise DNA and RNA sequencing. Increased read lengths enable complete gene isoform sequencing, gap closing of existing genome assemblies and sequencing and assembly of complete microbial genomes. This year’s TOAST workshop will be focused on whole genome sequencing using the handheld MinION sequencer developed by Oxford Nanopore. Significantly, the workshop will include hands-on sequencing and downstream data analysis.

Subtopics include:

Library prep and genome sequencing using a MinION

Basecalling live vs local

Assembly and error correction of long reads

Hybrid assembly & polishing of microbial genomes

Speakers:

Tonia Russel (Ramaciotti)

Benjamin Schwessinger (Australian National University)

Tim Kahlke (University of Technology, Sydney)

FAQ

Will food be provided?
Yes, a light lunch and tea and coffee will be provided. Please indicate your dietary requirements during registration.

Do I need pre-existing knowledge, e.g. in bioinformatics?No. Everyone with an interest in long-read sequencing can attend the course. However, pre-existing knowledge in command-line usage will help to get the most out of it.

What are the minimum computer requirements?A 64bit computer with a minimum of 2GB of memory with installed ssh client (Windows users).

Do I need to install software?Users with MAC or Unix operating systems will be able to do the analyses on their laptop. A list of tools will be provided before the course.Windows users can work remotely on UTS computers via ssh.

Where can I get an ssh clientFor Mac laptops please install XCode and XCode command-line tools.For Windows preferred ssh client is MobaXTERM. https://mobaxterm.mobatek.net/download.htmlUbuntu and other Unix-derivatives should come with ssh already installed.