A
10X UPlan
S-Apo N.A. 0.40 is also
available (w.d.
= 3.1 mm)-- ask Greg
to put it on if you want to use it.

Notes:

The 100X
oil objectives has an iris adjusted with a collar on the barrel of the
objective. The collar should be turned full clockwise.

The 40X dry objective
has
a cover slip correction collar. Cover slips vary in thickness with a # 1 1/2
coverslip being nominally .17 mm thick. Adjust the collar
for the best image: while looking at the specimen by eye, adjust the
collar in small increments, refocusing continuously with the microscope's fine
focus. Go for the highest contrast, "sharpest" image -- a small punctate
structure should become blurry in an identical fashion as you defocus both
above and below the structure.

The 40X and 60X
water immersion objectives have
a cover slip correction collar. Cover slips vary in thickness with a
# 1 1/2 coverslip being nominally .17 mm thick. Adjust the collar
for the best image: while looking at the specimen by eye, adjust the
collar in small increments, refocusing continuously with the microscope's
fine focus. Go for the highest contrast, "sharpest" image --
a small punctate structure should become blurry in an identical fashion
as you defocus both above and below the structure.