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Studier Induction ZYP-5052 Medium

Studier Induction Media is based on Studier’s ZYP-5052 media and has been reformulated for ease of use. All of the ingredients are contained in 2 bottles instead of the usual 5. This medium should be used when stronger buffering is needed. This media contains high amounts of phosphate which reduces the selectivity of kanamycin. The amount of kanamycin (when appropriate) used should therefore be increased to at 200 μg/ml.

At the time of usage, aseptically add 120 mL of Studier Salts Solution (#3S8600 120 mL included) to Studier ZYP-5052 Medium for Induction. (#3S8000 880 mL included). Media is stable for 2 months once the salt solution has been added. Note: this media contains glucose, lactose, and glycerol as carbon sources. No further additions are required. induction media works with all T7 regulated E. coli vectors in BL21 and derivative strains.

induction media works with all T7 regulated E. coli vectors in BL21 and derivative strains.