The inventiondiscloses a methodfor fresh fish liver tissue fixing based on precision-cut liver slices technology (PCLS),which goes like this: prepareagarosesolution of 10-50g/L, placethesolution in a container; when the solutiontemperature decreasesto 10-60¡æ, put thefresh livertissuecollected into it, and whentheagarosesolution iscompletely solidified, thefresh fish livertissue will also be fixed.This method is simple andconvenient,and can be widely used in scientific research.