AnaSpec, EGT Group's extensive range of MMP products includes the SensoLyte® MMP protease assay kits (FRET-based or colorimetric), antibodies, recombinant proteins and peptides. In response to popular demand to expand our SensoLyte® Plus line of kits, we are pleased to announce the recent release of the SensoLyte® Plus 520 MMP-2 assay kit. SensoLyte® Plus assays are designed specifically for detecting a certain MMP activity in biological sample which may contain multiple MMPs. A monoclonal anti-human-MMP antibody is employed to pull down the MMP of interest from the mixture first. The activity of the MMP is then quantified by a 5-FAM/QXL® 520 fluorescence resonance energy transfer (FRET) peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-induced cleavage of the FRET substrate. SensoLyte® 520 MMP Assay Kits which do not contain a pull down antibody are useful for screening of inhibitors using a purified MMP. With published results in world-class journals and the industry's
longest wavelength, the SensoLyte® Plus and SensoLyte® 520 line is one of the most sensitive
(Figure 2) and brightest lines of MMP assay kits in the world.

Figure 2. The performance of an identical peptide sequence,
one coupled with 5-FAM/QXL® 520 and the other with EDANS/DABCYL
FRET pairs was compared (A). A 5-FAM/QXL® 520 MMP substrate was compared with Mca/Dpa FRET substrate
(B). Fluorescence was measured after 1 hr incubation of substrates using serial
dilutions of MMP-13 (Flexstation 384II, Molecular Devices).

Fig. 4. WB of Z-Fish™ anti-MMP-13a antibody (cat# 55114). An immunoreactive band at ~54 kDa was detected in zebrafish 24 hpf embryo lysate (A) and zebrafish 5 dpf lysate (B). Two bands appeared at ~80 kDa and ~45 kDa in zebrafish lysate (5 dpf) (B) indicate the proenzyme and the cleavage from MMP-13. All the bands were blocked by the immunizing peptide (C and D).