Influence of medium composition on (S)-adenosyl-L-methionine and ergosterol content in biomass of the wild yeast strain Pichia stipitis and mutant M12

Granter

University of ZagrebFaculty of Food Technology and Biotechnology

Lower level organizational units

Department of Food EngineeringLaboratory for Fermentation and Yeast Technology

Place

Zagreb

State

Croatia

Scientific field, discipline, subdiscipline

BIOTECHNICAL SCIENCESBiotechnology

Study programme type

university

Study level

graduate

Study programme

Bioprocess Engineering

Academic title abbreviation

mag. ing. bioproc.

Genre

master's thesis

Language

Croatian

Defense date

2013-09-26

Parallel abstract (English)

(S)-adenosyl-L-methionine (SAMe) is an important bioactive molecule which participates in a number of biochemical reactions. Its absence or extortionate amount in organism can cause different diseases. Since this substance is used in treatment of liver diseases, depression and osteoarthritis, it's very important to find more efficient ways of SAMe production. In order to produce SAMe, wild yeast strain Pichia stipitis and mutant strain M12 were used. The aim of this work was to investigate the influence of medium composition on cell growth, ergosterol and SAMe content in yeasts cells. Both strains showed better growth and SAMe production in medium which contains magnesium and potassium salts, compared to medium which doesn’t contain these salts. Also, mutant strain growing on medium supplemented with salts produced much more SAMe compared to wild strain. 48,12 mg g-1 (SAMe / dry weight) was achieved with mutant strain, and only 26,05 mg g-1 with wild strain. It was also confirmed that wild strain P. stipitis has a better ethanol tolerance than mutant strain M12.