free floating sections

All,
I have been asked to stain free floating sections and am enlisting
my greatest resource. If possible we would like to use frozen tissue and
thick sections. The premise is that we can stain without the tissue ever
drying. Advice from anyone with experience would be greatly appreciated.
I'll take any ideas and promise not to be offended by sarcasm etc [polly
would definitely freeze here today]
TIA,
Cynthia Favara
Rocky Mountain Laboratories
903 S 4th Street
Hamilton, MT 59840
ph: 406-363-9317
FAX: 406-363-9286
e-mail: cfavara@nih.gov