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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is
committed to providing our customers around the world with the highest quality products.
In addition to direct selling in more than 20 countries in North America, Europe and
Asia/Pacific, Miltenyi Biotec also provides support for our customers through an
extensive distributor network covering dozens of additional countries.

As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is
committed to providing our customers around the world with the highest quality products.
In addition to direct selling in more than 20 countries in North America, Europe and
Asia/Pacific, Miltenyi Biotec also provides support for our customers through an
extensive distributor network covering dozens of additional countries.

Background information

IL-23 is a covalently linked heterodimeric cytokine, which is closely related to IL-12. Macrophages and dendritic cells are the major sources of both IL-12 and IL-23, which act on receptors primarily expressed by T cells, NK cells, and NKT cells. IL-23 consists of the p40 subunit shared with IL-12 and the 19 kDa protein p19 that is unique to IL-23. Its effects are mediated by a receptor composed of IL-12 receptor subunit β1 and an IL-23 specific subunit. IL-12 and IL-23 show different functions although both contain the p40 subunit. While IL-12 drives the development of T

H

1 cells, IL-23 mediates the full differentiation and maintenance of T

H

17 cells. IL-23 stimulates CD4

+

T cells and promotes the production of pro-inflammatory cytokines. As a key cytokine in the survival and proliferation of T

H

17 cells, IL-23 has central roles in autoimmune diseases and promotes chronic inflammation. It has been shown that single chain fusion proteins of naturally heterodimeric cytokines such as IL-12 or IL-23 are bioactive

in vitro

and

in vivo

.

Quality description

Research-grade

cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Figure 2

Figure 2

SDS-PAGE of Mouse IL-23

under reducing conditions (R). Units p40 and p19 are fused by a flexible linker region resulting in a single-chain polypeptide. The theoretical molecular mass is 58 kDa. Due to glycosylation the protein has an apparent mass of 70 kDa.

SDS-PAGE of Mouse IL-23

under reducing conditions (R). Units p40 and p19 are fused by a flexible linker region resulting in a single-chain polypeptide. The theoretical molecular mass is 58 kDa. Due to glycosylation the protein has an apparent mass of 70 kDa.