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Could you run other sample preparations (cell lysates or otherwise) alongside, to see if the gels separate out proteins successfully from other sources?

To be honest, there doesn't appear to be a problem with the gel at first glance, but I rarely run these types of samples. It is somewhat odd that nothing fills in those gaps, but it may be completely consistent with your samples and their preparation. For example, I would expect plasma to have distinct banding pattern as compared to total cell lysates.

Do you have an example of what your gels are predicted to look like? Perhaps this is the normal result for blood and plasma - Like I said, I'm not experienced with these samples, but perhaps others here are.

your "problem" may be due to the acrylamide percentage (12%). the higher molecular weight proteins are bunched up while the lower molecular weight proteins are more widely separated (look at your standards).

you can try a lower percentage gel (eg 10%, 7.5%) or a gradient to obtain more regular banding patterns.

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