The invention relates to pyrido[3,2-e]pyrazines, to processes for preparing them, to pharmaceutical preparations which comprise these compounds and to the pharmaceutical use of these compounds, which are inhibitors of phosphodiesterase 10, as active compounds for treating diseases of mammals including...http://www.google.it/patents/US7550465?utm_source=gb-gplus-shareBrevetto US7550465 - imidazo[1,5-a]pyrido[3,2-e]pyrazines; neurologic and psychiatric disorders, for example psychosis

The invention relates to pyrido[3,2-e]pyrazines, to processes for preparing them, to pharmaceutical preparations which comprise these compounds and to the pharmaceutical use of these compounds, which are inhibitors of phosphodiesterase 10, as active compounds for treating diseases of mammals including a human which can be influenced by using the compounds according to the invention to inhibit phosphodiesterase 10 activity in the central nervous system. More particularly, the invention relates to the treatment of neurologic and psychiatric disorders, for example psychosis and disorders comprising cognitive deficits as symptoms.

Immagini(10)

Rivendicazioni(11)

1. A compound of formula (II)

wherein the bond between A and N is a single bond or a double bond;

A is C when the bond is a double bond and CH when the bond is a single bond;

m is 0 or 1;

n is 0 or 1;

R1 and R2 are independently selected from

H,

a cyclic radical;

C1-8 alkyl, optionally mono- or polysubstituted with at least one of halo, OH, O—C1-3 alkyl or a cyclic radical;

C2-8 alkenyl, optionally mono- or polysubstituted with at least one of halo, OH, O—C1-3 alkyl or a cyclic radical;

C2-8 alkynyl, optionally mono- or polysubstituted with at least one of halo, OH, O—C1-3-alkyl or a cyclic radical;

a saturated, monounsaturated or polyunsaturated carbocycle ring system with 3 to 8 atoms or a heterocyclic ring with 5 to 15 ring atoms, each optionally mono- or polysubstituted with at least one of halo, amino, C1-3 alkylamino, di-C1-3 alkylamino, nitro, C1-3 alkyl, O—C1-3 alkyl or a cyclic radical; and

R3 is selected from

H,

a cyclic radical,

N3,

CN,

R6, OR6, SR6, SOR6,SO2R6,

NH(CO)OR6, N((CO)OR6)2, NR6((CO)OR6),

NH—(C═O)—NH2, NR6—(C═O)—NH2,

NH—(C═O)—NHR6, NR6—(C═O)—NHR6,

NH—SO2R6, N(SO2R6)2 and NR6(SO2R6),

wherein R6 is independently,

a cyclic radical,

C1-8 alkyl, C3-8 cyclo(hetero)alkyl,

C2-8 alkenyl, C3-8 cyclo(hetero)alkenyl,

or C2-8 alkynyl each optionally mono or polysubstituted with at least one of halo, OH, O—C1-3 alkyl or a cyclic radical,

wherein aryl is phenyl or naphthyl, heteroaryl is an aromatic heterocyclic ring system of 5 to 15 ring atoms containing at least one atom selected from N including N-oxide, S, and O and wherein aryl and heteroaryl are optionally mono- or polysubstituted with at least one of halo, amino, C1-3 alkylamino, di-C1-3 alkylamino, nitro, C1-3 alkyl, O—C1-3 alkyl or a cyclic radical and

R8 is C1-5 alkyl, optionally mono or polysubstituted with at last one of halo, OH, O—C1-3 alkyl or a cyclic radical,

R4 is selected from

H,

halo,

a cyclic radical,

R9,

OH or OR9,

NH(C═O)—C1-3 alkyl, optionally mono- or polysubstituted with at least one of halo, OH, O—C1-3 alkyl or a cyclic radical,

NH2, NHR9 or NR9R10,

wherein R9 and R10 are independently selected from a cyclic radical,

C1-6 alkyl or C3-6 cyclo(hetero)alkyl, optionally mono- or polysubstituted with at least one of halo, OH, O—C1-3 alkyl or a cyclic radical,

aryl-C1-5-alkyl wherein aryl is phenyl, optionally mono- or polysubstituted with at least one of halo, amino, C1-3 alkylamino, di-C1-3 alkylamino, nitro, C1-3 alkyl, O—C1-3 alkyl or a cyclic radical or

NR9R10 together form a saturated or unsaturated five-, six- or seven-membered ring which can contain up to 3 heteroatoms, preferably N including N-oxide, S or O, optionally mono- or polysubstituted with at least one of halo, amino, C1-3 alkylamino, di-C1-3 alkylamino, nitro, C1-3 alkyl, O—C1-3 alkyl or aryl-C1-5-alkyl, wherein aryl is phenyl, optionally mono- or polysubstituted with at least one of halo, amino, C1-3 alkylamino, di-C1-3 alkylamino, nitro, C1-3 alkyl, O—C1-3 alkyl or a cyclic radical,

and R5 is selected from

H,

C1-5 alkyl, C3-6 cycloalkyl or (CO)—C1-5 alkyl, optionally mono or polysubstituted with at least one of halo, OH, O—C1-3 alkyl or a cyclic radical,

or a pharmaceutically acceptable salt or prodrug thereof, wherein if m and n are 1,

A and N are not a double bond;

wherein if m is 1 and n is O, A and N are not a double bond;

wherein if n is 1 and m is O, A and N are not a single bond; and

wherein if m and n are O, A and N are not a single bond.

2. A compound according to claim 1 wherein the bond between A and N is a double bond.

3. A compound according to claim 1 wherein m and n are both 0.

4. A compound according to claim 1, wherein R1 is C2-4 alkyl or phenyl each optionally substituted.

5. A compound according to 1, wherein R2 is H, methyl or trifluoromethyl.

6. A compound according to claim 1 wherein R3 is H, —CN or C1-3 alkyl.

7. A compound according to claim 1, wherein R3 is —NH—(C═O)—OR6.

8. A compound according to claim 1, wherein R3 is —NH—SO2R6.

9. A compound according to claim 1, wherein R4 is H, C1-3 alkyl or O—C1-3 alkyl each optionally substituted.

10. A compound according to claim 1 selected from the group consisting of 4,8-dimethoxy-3-methyl-1-propyl-imidazo[1,5-a]pyrido[3,2-e]pyrazine;

11. A pharmaceutical composition comprising a compound of claim 1 and at least one of a pharmaceutically acceptable carrier, diluent or adjuvant.

Descrizione

This application claims priority from provisional U.S. Ser. No. 60/809,242 filed May 30, 2006, herein incorporated by reference in its entirety.

FIELD OF THE INVENTION

The invention relates to pyrido[3,2-e]pyrazines, to processes for preparing them, to pharmaceutical preparations which comprise these compounds and to the pharmaceutical use of these compounds, which are inhibitors of phosphodiesterase 10, as active compounds for treating diseases of mammals including a human which can be influenced by using the compounds according to the invention to inhibit phosphodiesterase 10 activity in the central nervous system. More particularly, the invention relates to the treatment of neurologic and psychiatric disorders, for example psychosis and disorders comprising cognitive deficits as symptoms.

BACKGROUND

Psychotic disorders, especially schizophrenia, are severe mental disorders which extremely impair daily life. The symptoms of psychosis may be divided into two fractions. In the acute phase, it is predominated by hallucinations and delusions being called the positive symptoms. When the agitated phase abates the so called negative symptoms become obvious. They include cognitive deficits, social phobia, reduced vigilance, indifference and deficits in verbal learning and memory, verbal fluency and motor function.

Although several antipsychotics are available since, the present therapy of psychosis is not satisfactory. The classic antipsychotics, such as haloperidol, with a high affinity to dopamine D2 receptor show extreme side effects, such extrapyramidal symptoms (=EPS) and do not improve the negative symptoms of schizophrenia so that they do not enable the patient to return to everyday life.

Clozapine which has emerged as a benchmark therapeutic ameliorating positive, negative and cognitive symptoms of schizophrenia and devoid of EPS shows agranulocytosis as a major, potential lethal side-effect (Capuano et al., 2002). Besides, there is still a high amount of therapy resistant cases (Lindenmayer et al., 2002).

In conclusion, there is still a need for developing new antipsychotics which ameliorate positive, negative and cognitive symptoms of psychosis and have a better side effect profile.

The exact pathomechanism of psychosis is not yet known. A dysfunction of several nreurotransmitter systems has been shown. The two major neurotransmitter systems that are involved are the dopaminergic and the glutamatergic system:

Thus, acute psychotic symptoms may be stimulated by dopaminergic drugs (Capuano et al., 2002) and classical antipsychotics, like haloperidol, have a high affinity to the dopamine D2 receptor (Nyberg et al., 2002). Animal models based on a hyperactivity of the dopaminergic neurotransmitter system (amphetamine hyperactivity, apomorphine climbing) are used to mimic the positive symptoms of schizophrenia.

Additional there is growing evidence that the glutamatergic neurotransmitter system plays an important role in the development of schizophrenia (Millan, 2005). Thus, NMDA antagonists like phencyclidine and ketamine are able to stimulate schizophrenic symptoms in humans and rodents (Abi-Saab et al., 1998; Lahti et al., 2001). Acute administration of phencyclidine and MK-801 induce hyperactivity, stereotypies and ataxia in rats mimicking psychotic symptoms. Moreover, in contrast to the dopaminergic models the animal models of psychosis based on NMDA antagonists do not only mimic the positive symptoms but also the negative and cognitive symptoms of psychosis (Abi-Saab et al., 1998; Jentsch and Roth, 1999). Thus, NMDA antagonists, additionally induce cognitive deficits and social interaction deficits.

Eleven families of phosphodiesterases have been identified in mammals so far (Essayan, 2001). The role of PDEs in the cell signal cascade is to inactivate the cyclic nucleotides cAMP and/or cGMP (Soderling and Beavo, 2000). Since cAMP and cGMP are important second messenger in the signal cascade of G-protein-coupled receptors PDEs are involved in a broad range of physiological mechanisms playing a role in the homeostasis of the organism.

The PDE families differ in their substrate specificity for the cyclic nucleotides, their mechanism of regulation and their sensitivity to inhibitors. Moreover, they are differentially localized in the organism, among the cells of an organ and even within the cells. These differences lead to a differentiated involvement of the PDE families in the various physiological functions.

PDE10A is primarily expressed in the brain and here in the nucleus accumbens and the caudate putamen. Areas with moderate expression are the thalamus, hippocampus, frontal cortex and olfactory tubercle (Menniti et al., 2001). All these brain areas are described to participate in the pathomechanism of schizophrenia (Lapiz et al. 2003) so that the location of the enzyme indicates a predominate role in the pathomechanism of psychosis.

In the striatum PDE10A is predominately found in the medium spiny neurons and there are primarily associated to the postsynaptic membranes of these neurons (Xie et al., 2006). By this location PDE10A may have an important influence on the signal cascade induced by dopaminergic and glutamatergic input on the medium spiny neurons two neurotransmitter systems playing a predominate role in the pathomechanism of psychosis.

Psychotic patients have been shown to have a dysfunction of cGMP and cAMP levels and its downstream substrates (Kaiya, 1992; Muly, 2002; Garver et al., 1982). Additionally, haloperidol treatment has been associated with increased cAMP and cGMP levels in rats and patients, respectively (Leveque et al., 2000; Gattaz et al., 1984). As PDE10 hydrolyses both cAMP and cGMP (Kotera et al., 1999) an inhibition of PDE10A would also induce an increase of cAMP and cGMP and thereby having a similar effect on cyclic nucleotide levels as haloperidol.

The antipsychotic potential of PDE10A inhibitors is further supported by studies of Kostowski et al. (1976) who showed that papaverine, a moderate selective PDE10A inhibitor, reduces apomorphine-induced stereotypies in rats, an animal model of psychosis, and increases haloperidol-induced catalepsy in rats while concurrently reducing dopamine concentration in rat brain. Activities that are also seen with classical antipsychotics. This is further supported by a patent application establishing papaverine as a PDE10A inhibitor for the treatment of psychosis (US Patent Application No. 2003/0032579).

In addition to classical antipsychotics which mainly ameliorate the positive symptoms of psychosis PDE10A also bears the potential to improve the negative and cognitive symptoms of psychosis.

Elevated intracellular cAMP levels mediated by D1 receptor signalling seems to modulate a series of neuronal processes responsible for working memory in the prefrontal cortex (Sawaguchi, 2000), and it is reported that D1 receptor activation may improve working memory deficits in schizophrenic patients (Castner et al., 2000). Thus, it seems likely that a further enhancement of this pathway might also improve the cognitive symptoms of schizophrenia.

Further indication of an effect of PDE10A inhibition on negative symptoms of psychosis are given by Rodefer et al. (2005) who could show that papaverine reverses attentional set-shifting deficits induced by subchronic administration of phencyclidine, an NMDA antagonist, in rats. Attentional deficits including an impairment of shifting attention to novel stimuli belongs to the negative symptoms of schizophrenia. In the study the attentional deficits were induced by administering phencyclidine for 7 days followed by a washout period. The PDE10A inhibitor papaverine was able to reverse the enduring deficits induced by the subchronic treatment.

Imidazo[1,5-a]pyrido[3,2-e]pyrazinones its synthesis and some medical uses are well described in patents and the literature.

The applications EP 0 400 583 and U.S. Pat. No. 5,055,465 from Berlex Laboratories, Inc. disclose a group of imidazoquinoxalinones, their aza analogs and a process for their preparation. These compounds have been found to have inodilatory, vasodilatory and venodilatory effects. The therapeutic activity is based on the inhibition of phosphodiesterase 3 (PDE3).

EP 0 736 532 discloses pyrido[3,2-e]pyrazinones and a process for their preparation. These compounds are described to have anti-asthmatic and anti-allergic properties. Examples of this invention are inhibitors of PDE4 and PDE5.

WO 00/43392 discloses the use of imidazo[1,5-a]pyrido[3,2-e]pyrazinones which are inhibitors of PDE3 and PDE5 for the therapy of erectile dysfunction, heart failure, pulmonic hypertonia and vascular diseases which are accompanied by insufficient blood supply.

An other group of pyrido[3,2-e]pyrazinones, disclosed in WO 01/68097 are inhibitors of PDE5 and can be used for the treatment of erectile dysfunction.

Further methods for the preparation of imidazo[1,5-a]pyrido[3,2-e]pyrazinones are described also by D. Norris et al. (Tetrahedron Letters 42 (2001), 4297-4299).

WO 92/22552 refers to imidazo[1,5-a]quinoxalines which are generally substituted at position 3 with a carboxylic acid group and derivatives thereof. These compounds are described to be useful as anxiolytic and sedative/hypnotic agents.

In contrast only a limited number of imidazo[1,5-a]pyrido[3,2-e]pyrazines and their medical use are already published.

WO 99/45009 describes a group of imidazopyrazines of formula (I)

Part of the definition of Q is to form a 6-membered heterocyclic ring including pyridin. While R1, R2 and R3 are representing a large variety of substituents the definition of the group —NR4R5 is of special importance.

R4 and R5 are each independently hydrogen, R6 or —C(O)R6 or the whole group NR4R5 forms a 3- to 8-membered saturated or unsaturated ring.

R6 is alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkylalkyl, cycloalkenyl, cycloalkenylalkyl, aryl, aralkyl, heterocyclo or heterocycloalkyl, each of which is unsubstituted or substituted.

These compounds are described to be inhibitors of protein tyrosine kinases used in the treatment of protein tyrosine kinase-associated disorders such as immunologic disorders.

Interestingly, for all examples listed in claim 9 the structure of the group NR4R5 is limited in a way that one of R4 and R5 is hydrogen and for the other one R6 is phenyl (unsubstituted or substituted).

This structural selection of the group NR4R5 is inline with published SAR data from the same company (P. Chen et al., Bioorg. Med. Chem. Lett. 12 (2002), 1361-1364 and P. Chen et al., Bioorg. Med. Chem. Lett. 12 (2002), 3153-3156).

SUMMARY OF THE INVENTION

This invention relates to compounds of formula (II) and to pharmaceutically acceptable salts, solvates and prodrugs thereof.

wherein the bond between A and N is a single bond or a double bond,

A is C when the bond is a double bond and CH when the bond is a single bond,

a saturated, monounsaturated or polyunsaturated carboxylic ring system with 3 to 8 atoms, e.g. phenyl, or a heterocyclic ring system with 5 to 15 ring atoms containing at least one heteroatom selected from N including N-oxide, O and S, each optionally mono- or polysubstituted with halo, amino, C1-3 alkylamino, di-C1-3 alkylamino, nitro, C1-3 alkyl, O—C1-3 alkyl, and/or a cyclic radical, and

FIG. 4 is a protein alignment showing difference in the protein sequences without the catalytic domain of PDE10 for rat, guinea pig and pig.

FIG. 5 shows the effect of the compounds of Examples 91, 35, 95 and 55 on MK-801-induced psychosis.

FIG. 6 shows the effect of the compounds of Example 38 and 47 on MK-801-induced psychosis.

FIG. 7 shows the effect of the compounds of Example 62 and 69 on MK-801 induced psychosis.

FIG. 8 shows the effect of the compounds of Example 24 and 30 on MK-801 induced psychosis.

DETAILED DESCRIPTION

The term “halo” refers to fluoro, chloro, bromo or iodo.

The terms “alkyl”, “alkenyl” and “alkynyl” refer to straight or branched radicals with up to 8 carbon atoms preferably up to 6 carbon atoms and more preferably up to 5 carbon atoms such as methyl, ethyl, vinyl, ethynyl, propyl, allyl, propynyl, butyl, butenyl, butynyl etcl. which may optionally be substituted as indicated above.

The terms “cyclo(hetero)alkyl” and “cyclo(hetero)alkyenyl” refer to cyclic radicals' which may optionally contain one or more heteroatoms selected from N including N-oxide, O and S, which may optionally be substituted as indicated above.

The term “cyclic radical” refers to saturated, unsaturated or aromatic carbocyles or carboheterocycles, optionally mono- or polysubstituted with halo, amino, C1-3 alkylamino, di-C1-3 alkylamino, nitro, C1-3 alkyl, OH, O—C1-3 alkyl and/or a cyclic radical. The cyclic radical preferably contains 3 to 20, in particular 4 to 10 C-atoms. Carboheterocyles may contain 1 to 6, in particular 1 to 3 heteroatoms, preferably selected from O, N, S and/or P. The cyclic radical can be bound via a C-atom or optionally via a N, O, S, SO or SO2-group. An example for a cyclic radical is phenyl.

A preferred embodiment of this invention relates to compounds of formula (II) wherein the bond between A and N is a double bond.

An other preferred embodiment of this invention relates to compounds of formula (II) wherein m and n are both 0.

A further preferred embodiment of this invention relates to compounds of formula (II) wherein R1 is selected from

Especially preferred are hydrogen, a methyl group or a trifluoromethyl group.

A further preferred embodiment of this invention relates to compounds of formula (II) wherein R3 is H, CN or C1-3 alkyl, e.g. methyl.

A further preferred embodiment of this invention relates to compounds of formula (II) wherein R3 is NH—(C═O)OR6, particularly NH—(C═O)—OC1-5 alkyl, optionally mono- or polysubstituted as indicated above.

A further preferred embodiment of this invention relates to compounds of formula (II) wherein R3 is NH—SO2R6, particularly NH—SO2—C1-5 alkyl, optionally mono- or polysubstituted as indicated above.

A further preferred embodiment of this invention relates to compounds of formula (II) wherein R4 is selected from

Especially preferred, the compound of formula (II) is selected from 3,4-dimethyl-8-methoxy-1-propyl-imidazo[1,5-a]-pyrido[3,2-e]-pyrazine and pharmaceutically acceptable salts and derivatives thereof.

The invention furthermore relates to the physiologically acceptable salts, solvates and derivatives of the compounds according to formula (II). Derivatives of the compounds according to formula (II) are, for example, amides, esters and ethers. Further, the term “derivative” also encompasses prodrugs and metabolites of compounds of formula (II).

In addition, physiologically acceptable salts of the compounds according to formula (II) can be obtained by converting derivatives which possess tertiary amino groups into the corresponding quaternary ammonium salts in a manner known per se using quaternizing agents. Examples of suitable quaternizing agents are alkyl halides, such as methyl iodide, ethyl bromide and n-propyl chloride, and also arylalkyl halides, such as benzyl chloride or 2-phenylethyl bromide.

Furthermore, in the case of the compounds of the formula (II) which contain an asymmetric carbon atom, the invention relates to the D form, the L form and D,L mixtures and also, where more than one asymmetric carbon atom is present, to the diastereomeric forms. Those compounds of the formula (II) which contain asymmetric carbon atoms, and which as a rule accrue as racemates, can be separated into the optically active isomers in a known manner, for example using an optically active acid. However, it is also possible to use an optically active starting substance from the outset, with a corresponding optically active or diastereomeric compound then being obtained as the end product.

The compounds according to the invention have been found to have pharmacologically important properties which can be used therapeutically. The compounds according to formula (II) can be used alone, in combination with each other or in combination with other active compounds. The compounds according to the invention are inhibitors of phosphodiesterase 10. It is therefore a part of the subject-matter of this invention that the compounds according to formula (II), and their salts and also pharmaceutical preparations which comprise these compounds or their salts, can be used for treating or preventing discorders associated with, accompanied by and/or covered by phosphodiesterase hyperactivity and/or disorders in which inhibiting phosphodiesterase 10 is of value.

Surprisingly, the compounds of formula (II) are potent inhibitors of the enzyme PDE10.

It is an embodiment of this invention, that compounds of formula (II) including their salts, solvates and prodrugs and also pharmaceutical compositions comprising an amount of a compound of formula (II) or one of its salts, solvates or prodrugs effective in inhibiting PDE10 can be used for the treatment of central nervous system disorders of mammals including a human.

(2) Examples of mood [affective] disorders that can be treated according to the present invention include, but are not limited to, manic episodes associated to bipolar disorder and single manic episodes, hypomania, mania with psychotic symptoms; bipolar affective disorders (including for instance bipolar affective disorders with current hypomanic and manic episodes with or without psychotic symptoms); depressive disorders, such as single episode or recurrent major depressive disorder, depressive disorder with postpartum onset, depressive disorders with psychotic symptoms; persistent mood [affective] disorders, such as cyclothymia, dysthymia; premenstrual dysphoric disorder.

(3) Examples of disorders belonging to the neurotic, stress-related and somatoform disorders that can be treated according to the present invention include, but are not limited to, phobic anxiety disorders, for instance agoraphobia and social phobia primarily but not exclusively related to psychosis; other anxiety disorders such as panic disorders and general anxiety disorders; obsessive compulsive disorder; reaction to sever stress and adjustment disorders, such as post traumatic stress disorder; dissociative disorders and other neurotic disorders such as depersonalisation-derealisation syndrome.

(6) Examples of disorders usually first diagnosed in infancy, childhood and adolescence that can be treated according to the present invention include, but are not limited to, hyperkinetic disorders, attentional deficit/hyperactivity disorder (AD/HD), conduct disorders; mixed disorders of conduct and emotional disorders; nonorganic enuresis, nonorganic encopresis; stereotyped movement disorder; and other specified behavioural emotional disorders, such as attention deficit disorder without hyperactivity, excessive masturbation nail-biting, nose-picking and thumb-sucking; disorders of psychological development particularly schizoid disorder of childhood and pervasive development disorders such as psychotic episodes associated to Asperger's syndrome.

(8) Examples of disorders of psychological development include but are not limited to developmental disorders of speech and language, developmental disorders of scholastic skills, such as specific disorder of arithmetical skills, reading disorders and spelling disorders and other learning disorders. These disorders are predominantly diagnosed in infancy, childhood and adolescence.

(9) The phrase “cognitive deficiency” as used here in “disorder comprising as a symptom cognitive deficiency” refers to a subnormal functioning or a suboptimal functioning in one or more cognitive aspects such as memory, intellect, learning and logic ability, or attention in a particular individual comparative to other individuals within the same general age population.

(11) Additionally, the invention relates to movement disorders with malfunction of basal ganglia. Examples of movement disorders with malfunction of basal ganglia that can be treated according to the present invention include, but are not limited to, different subtypes of dystonia, such as focal dystonias, multiple-focal or segmental dystonias, torsion dystonia, hemispheric, generalised and tardive dyskinesias (induced by psychopharmacological drugs), akathisias, dyskinesias such as Huntington's disease, Parkinson's disease, Lewis body disease, restless leg syndrome, PLMS.

(12) Furthermore the invention relates to the treatment of organic, including symptomatic mental disorders, especially to organic delusional (schizophrenia-like) disorders, presenil or senile psychosis associated to dementia, to psychosis in epilepsy and Parkinson's disease and other organic and symptomatic psychosis; delirium; infective psychosis; personality and behavioural disorders due to brain disease, damage and dysfunction.

(13) The invention relates to the treatment of mental and behavioural disorders due to psychoactive compounds, more particular to the treatment of psychotic disorders and residual and late-onset psychotic disorders induced by alcohol, opioids, cannabinoids, cocaine, hallucinogens, other stimulants, including caffeine, volatile solvents and other psychoactive compounds.

(14) The invention further relates to a general improvement of learning and memory capacities in a mammal, including a human.

An effective dose of the compounds according to the invention, or their salts, is used, in addition to physiologically acceptable carriers, diluents and/or adjuvants for producing a pharmaceutical composition. The dose of the active compounds can vary depending on the route of administration, the age and weight of the patient, the nature and severity of the diseases to be treated, and similar factors. The daily dose can be given as a single dose, which is to be administered once, or be subdivided into two or more daily doses, and is as a rule 0.001-2000 mg. Particular preference is given to administering daily doses of 0.1-500 mg, e.g. 0.1-100 mg.

Cellulose ethers which can dissolve or swell both in water or in organic solvents, such as hydroxypropylmethyl cellulose, methyl cellulose or ethyl cellulose, or soluble starches, can be used as film-forming agents.

Mixtures of gelatinizing agents and film-forming agents are also perfectly possible. In this case, use is made, in particular, of ionic macromolecules such as sodium carboxymethyl cellulose, polyacrylic acid, polymethacrylic acid and their salts, sodium amylopectin semiglycolate, alginic acid or propylene glycol alginate as the sodium salt, gum arabic, xanthan gum, guar gum or carrageenan. The following can be used as additional formulation aids: glycerol, paraffin of differing viscosity, triethanolamine, collagen, allantoin and novantisolic acid. Use of surfactants, emulsifiers or wetting agents, for example of Na lauryl sulphate, fatty alcohol ether sulphates, di-Na—N-lauryl-β-iminodipropionate, polyethoxylated castor oil or sorbitan monooleate, sorbitan monostearate, polysorbates (e.g. Tween), cetyl alcohol, lecithin, glycerol monostearate, polyoxyethylene stearate, alkylphenol polyglycol ethers, cetyltrimethylammonium chloride or mono-/dialkylpolyglycol ether orthophosphoric acid monoethanolamine salts can also be required for the formulation. Stabilizers, such as montmorillonites or colloidal silicic acids, for stabilizing emulsions or preventing the breakdown of active substances such as antioxidants, for example tocopherols or butylhydroxyanisole, or preservatives, such as p-hydroxybenzoic acid esters, can likewise be used for preparing the desired formulations.

Preparations for parenteral administration can be present in separate dose unit forms, such as ampoules or vials. Use is preferably made of solutions of the active compound, preferably aqueous solution and, in particular, isotonic solutions and also suspensions. These injection forms can be made available as ready-to-use preparations or only be prepared directly before use, by mixing the active compound, for example the lyophilisate, where appropriate containing other solid carrier substances, with the desired solvent or suspending agent.

Intranasal preparations can be present as aqueous or oily solutions or as aqueous or oily suspensions. They can also be present as lyophilisates which are prepared before use using the suitable solvent or suspending agent.

Inhalable preparations can present as powders, solutions or suspensions. Preferably, inhalable preparations are in the form of powders, e.g. as a mixture of the active ingredient with a suitable formulation aid such as lactose.

The preparations are produced, aliquoted and sealed under the customary antimicrobial and aseptic conditions.

As indicated above, the compounds of the invention may be administered as a combination therapy with further active agents, e.g. therapeutically active compounds useful in the treatment of central nervous system disorders. These further compounds may be PDE10 inhibitors or compounds which have an activity which is not based on PDE10 inhibition such as dopamine D2 receptor modulating agents or NMDA modulating agents.

For a combination therapy, the active ingredients may be formulated as compositions containing several active ingredients in a single dose form and/or as kits containing individual active ingredients in separate dose forms. The active ingredients used in combination therapy may be co-administered or administered separately.

The synthesis of compounds of formula (II) preferably starts from imidazo[1,5-a]pyrido[3,2-e]pyrazinones of formula (III):

wherein R1, R2 and R4 are as described above.

The preparation of compounds of formula (III) is well described e.g. in WO 00/43392, WO 01/68097 and also by D. Norris et al. (Tetrahedron Letters 42 (2001), 4297-4299).

According to standard procedures known from the literature and already used in WO 99/45009 compounds of formula (III) are halogenated by treatment with halogenating reagents like POCl3, PCl3, PCl5 SOCl2, POBr3, PBr3 or PBr5, yielding e.g. 4-chloro or 4-bromo-imidazo[1,5-a]pyrido[3,2-e]pyrazines of formula (IV),

wherein X is Cl or Br and R1, R2 and R4 are as defined above.

Compounds of formula (II) where m and n are 0, the bond between A and N is a double bond and R3 is selected from OR6, SR6, OR7 or SR7 as described above, are preferably prepared by the treatment of an intermediate of formula (IV) with the corresponding alcohols or mercaptanes HOR6, HOR7, HSR6 or HSR7.

16 g of 8-methoxy-3-methyl-1-propyl-imidazo[1,5-a]pyrido[3,2-e]pyrazine-4-one and 120 ml POCl3 are mixed and heated up to reflux for 8 hours. After cooling to room temperature the reaction mixture is treated with 1200 ml crushed ice/water and stirred for 1 hour. The product is extracted with 2×300 ml dichloromethane. The collected organic layer is washed with 2×300 ml water and dried with Na2SO4. The solvent is removed under reduced pressure.

Yield: 14.5 g

m.p.: 121-123° C.

Many other intermediates A of formula (IV) can be prepared according to this procedure. Some examples are the following:

1.5 g of intermediate A1 are dissolved in a mixture of 15 ml methanol and 15 ml dichloromethane. 1 g of solid KOH is added. The mixture is heated up to reflux for 7 hours. At room temperature 30 ml water are added. The organic layer is separated. The aqueous layer is extracted with 20 ml dichloromethane. The unified organic layers are washed with 2×20 ml water. The solvent is removed completely. The residue is purified by LC.

Yield: 1.2 g

m.p.: 112-115° C.

The following examples are prepared using the same route of synthesis and reaction conditions like described above for example 1:

Example

R1

R2

R3

R4

m.p. [° C.]

1

—C3H7

—CH3

—OCH3

—OCH3

112-115

2

—C3H7

—H

—OCH3

—OCH3

113-116

3

—C2H5

—CH3

—OCH3

—OCH3

155-157

4

—CH3

—CH3

—OCH3

—OCH3

184-186

5

—H

—CH3

—OCH3

—OCH3

152-154

6

—C2H5

—CH3

—OCH(CH3)2

—OCH3

80-81

7

—C2H5

—CH3

—OC3H7

—OCH3

78-81

8

—C2H5

—CH3

—OCH3

76-78

9

—C3H7

—CH3

—OCH(CH3)2

—OCH3

78-80

10

—CH3

—CH3

—OCH3

227-229

11

—C2H5

—CH3

—OCH3

193-195

12

—C2H5

—CH3

—OCH3

149-151

13

—C2H5

—CH3

—OCH3

158-160

14

—C2H5

—CH3

—OCH3

157-160

15

—C3H7

—CH3

—OCH3

163-165

16

—C3H7

—CH3

—OCH3

147-149

17

—C2H5

—CH3

—OCH3

133-135

18

—C3H7

—CH3

—OCH3

129-132

19

—CH3

—CH3

—OCH3

115-118

20

—H

—CH3

—OCH3

111-114

21

—C3H7

—CH3

—OCH3

87-89

22

—C2H5

—CH3

—OCH3

75-78

23

—CH3

—CH3

—OCH3

83-85

24

—C2H5

—CH3

—OCH3

173-175

25

—C2H5

—CH3

—SCH3

—OCH3

156-159

26

—C3H7

—CH3

—SCH3

—OCH3

112-115

27

—CH3

—CH3

—SCH3

—OCH3

140-144

28

—H

—CH3

—SCH3

—OCH3

185-187

Compounds of formula (II) where m and n are 0, the bond between A and N is a double bond and R3 is —CN are preferably prepared by the treatment of an intermediate of formula (IV) with the Grignard reagent ethoxycarbonyl-difluoromethyl magnesium chloride followed by the substitution with a cyanide salt, e.g. KCN.

3 g of intermediate A1 are added into a solution of 32 g ethoxycarbonyl-difluoromethyl magnesia chloride in 100 ml tetrahydrofurane (THF). The mixture is stirred and heated up to reflux for 10 hours. Then the solvent is removed and 15 ml N,N-dimethylformamide and 2 g KCN are added. This reaction mixture is heated up to reflux for 5 hours. After this time 100 ml toluol are added. The organic layer is washed with 3×50 ml water. The solvent is removed and purified by preparative HPLC.

Yield: 0.2 g

m.p.: 178-180° C.

Using the same procedure and reaction conditions like described above for Example 29 also Example 30 was synthesized.

Compounds of formula (II) where m and n are 0, the bond between A and N is a double bond and R3 is —N3 are prepared by the treatment of an intermediate of formula (IV) with and an azide salt, e.g. NaN3.

1.5 g of intermediate A1 are stirred into 10 ml N,N-dimethylformamide. 1 g NaN3 is added at room temperature. The mixture is heated up to 60° C. and stirred for 5 hours. 100 ml toluol are added. The organic layer is separated and washed with 3×30 ml water. 90 ml of the solvent are removed. The reaction product precipitates. The crude product is purified by crystallisation from toluol.

Yield: 1.2 g

m.p.: >205° C. (decomp.)

Compounds of formula (II) where m and n are 0, the bond between A and N is a double bond and R3 is (SO)R6 or (SO2)R6, wherein R6 is as defined above, are prepared by oxidation of the corresponding compounds of formula (II) where R3 means —SR6.

0.7 g of 8-methoxy-3-methyl-4-methylthio-1-propyl-imidazo[1,5-a]pyrido[3,2-e]pyrazine (Example 26) are dissolved in 40 ml dichloromethane. 0.8 g of 3-chloroperoxybenzoic acid are added at 0 to 5° C. in small portions. The mixture is stirred for 2 hours at room temperature. The solution is washed with 2×30 ml saturated NaHCO3 solution and than with 2×30 ml water. The solvent is removed from the isolated organic layer. The crude mixture of Example 32 and Example 33 is separated by preparative HPLC.

Example 32

Yield: 0.2 g

m.p.: 144-147° C.

Example 33

Yield: 0.25 g

m.p.: 42-46° C.

Example 34 is prepared using the same route of synthesis and reaction conditions like described above for example 31:

Compounds of formula (II) where m and n are 0, the bond between A and N is a double bond and R3 is hydrogen are preferably prepared by the hydrogenation of an intermediate of formula (IV), e.g. with hydrogen in the presence of a catalyst such as palladium.

2 g of intermediate A1 are suspended in 50 ml ethanol. 1 ml triethylamine and 1 g palladium catalyst are added. An autoclave is used as reaction vessel. Hydrogen is pressed in up to 20 bar pressure. Now, the mixture is stirred at 30° C. for 4 hours. After filtration the solvent is removed. The crude product is dissolved in 100 ml dichloromethane. This solution is washed with 50 ml water. The solvent is removed to isolate pure product.

Yield: 1.3 g

m.p.: 134-135° C.

Using the same procedure and reaction conditions like described above for Example 35 also Example 36 was synthesized.

7 g of intermediate A1 are suspended in 150 ml tetrahydrofurane. 30 ml of a solution of ethyl magnesium bromide in tetrahydrofurane (3 M) are added. The mixture is stirred for 4 hours at room temperature. After filtration the solvent is removed. The crude product is purified by preparative HPLC.

Yield: 5.1 g

m.p.: 78-81° C.

The following compounds are prepared using the same route of synthesis and reaction conditions like described above for Example 37:

Example

R1

R2

R3

R4

m.p. [° C.]

37

—C3H7

—CH3

—C2H5

—OCH3

78-81

38

—C3H7

—CH3

—CH3

—OCH3

91-93

39

—C3H7

—CH3

—CH3

—OCH3

171-175

(× HCl)

40

—C2H5

—CH3

—CH3

—OCH3

106-109

41

—CH3

—CH3

—CH3

—OCH3

157-161

42

—CH2CH2CF3

—CH3

—CH3

—OCH3

145-147

43

—C5H11

—CH3

—CH3

—OCH3

70-71

44

—C6H11

—CH3

—CH3

—OCH3

149-152

45

—C6H13

—CH3

—CH3

—OCH3

73-75

46

—(CH2)2C6H5

—CH3

—CH3

—OCH3

121.5-123

47

—C6H5

—CH3

—CH3

—OCH3

189-192

48

—C6H5

—CH3

—CH3

—OCH3

210-218

(×2 HCl)

49

—C6H4(2-Cl)

—CH3

—CH3

—CH3

220-222

50

—C6H4(4-F)

—CH3

—CH3

—OCH3

235-238

51

—C3H7

—CH3

—CH3

—CH3

104-107

52

—C3H7

—CH3

—CH3

—H

92-95

53

—C3H7

H

—CH3

—OCH3

124-126

54

—C3H7

—CH3

—CH3

—OCHF2

126-130

55

—C3H7

—CH3

—CH3

98-101

56

—C2H5

—CH3

—CH3

146-149

57

—C2H5

—CH3

—CH3

73-75

58

—C3H7

—CH3

—CH3

105-107

An analogous compound with R3═CH3 was obtained during the synthesis of the above described of intermediate A28. Separation of the obtained 3 alkylated products by preparative chromatography resulted in Example 59.

Compounds of formula (II) where m is 0, n=1 and the bond between A and N is a double bond are synthesized from compounds of formula (II) where m and n are 0, the bond between A and N is a double bond by oxidation, e.g. with 3-chloroperoxybenzoic acid.

6 g of 8-methoxy-3-methyl-1-propyl-imidazo[1,5-a]pyrido[3,2-e]pyrazine (Example 35) are dissolved in 300 ml dichloromethane. A solution of 12 g 3-chloroperoxybenzoic acid in 40 ml acetic acid is added in small portions during 30 minutes. The reaction mixture is stirred for 16 hours at room temperature. Than the solution is washed with 2×50 ml saturated NaHCO3 solution and with 50 ml water. The solvent is removed. The crude product is purified by preparative HPLC.

Yield: 1.5 g

m.p.: 228-232° C.

The same route of synthesis and reaction conditions like described above for Example 37 were used for the synthesis of Example 42.

Compounds of formula (II) where m and n are 0, the bond between A and N is a double bond and R3 is NH(CO)OR6, N((CO)OR6)2, N(R6)((CO)OR6), NH(CO)NH2, NH(CO)NHR6, NR6(CO)NH2 and NR6(CO)NHR6

are preferably prepared by treatment of an intermediate of formula (IV) with NH3 or an alkyl amine, e.g. a C1-5 alkyl amine to form the corresponding 4-amino derivatives (according to the method from WO 99/45009). These 4-amino derivatives (intermediates B) are treated with suitable reagents such as chloro formic acid esters or amides to prepare the final products.

10 g of intermediate A1 and 200 ml of an aqueous solution of NH3 (32%) are mixed in an autoclave and heated up to 130° C. for 8 hours. The reaction mixture is diluted with 200 ml water. The precipitated reaction product is separated washed with water and dichloro methane and dried at reduced pressure.

1.4 g of the intermediate B1 are stirred with 20 ml dichloromethane 5 ml methanol and 1 ml triethylamine. At 0° C. a solution of 0.6 g chloro formic acid methylester in 10 ml dichloromethane is added slowly. The mixture is stirred for 2 hours at 0° C. Than the solution is heated up to reflux 10 hours. The solution is washed with 30 ml saturated NaHCO3 solution and with 30 ml water. The solvent is removed. The crude product is purified by preparative HPLC.

Yield: 0.22 g

m.p.: 137-138° C.

Further Examples prepared using the same route of synthesis and reaction conditions like described above for Example 62 are the following:

543 mg of Intermediate B1 and 960 mg N,N′-carbonyldiimidazole were stirred with 20 ml tetrahydrofurane for 3 hours under reflux. At room temperature 3 ml 40% methylamine solution was added slowly. The solution was heated up to reflux 30 minutes. After removing the solvent under reduced pressure the residue was extracted with 50 ml dichloromethane and 2×25 ml water. The organic layer is removed. The crude product was purified by preparative HPLC.

Yield: 0.4 g

m.p.: 178-181° C.

Further Examples prepared using the same route of synthesis and reaction conditions like described above for Example 66 are the following:

Compounds of formula (II) where m and n are 0, the bond between A and N is a double bond and R3 is NH—SO2R6, N(SO2R6)2, N(R6)(SO2R6), NHSO2R7, N(SO2R7)2 and N(R8)SO2R7, wherein R6, R7 and R8 are as defined above,

are preferably prepared by treatment of an intermediate of formula (IV) with NH3 or an alkyl amine, e.g. a C1-5 alkyl amine to form the corresponding 4-amino derivatives according to the method from WO 99/45009. These 4-amino derivatives (intermediates B) are treated with sulfonic acid chlorides or anhydrides forming the final sulfonamides.

10 g of the intermediate B1 are mixed with 350 ml toluol and 14 g methylsulfonic acid anhydride. The mixture is heated up to reflux for 1 hour. After this time 16 ml triethylamine are added at 70° C. The mixture is stirred then for 1 hour. 100 ml water are added. The product precipitates. After filtration it is washed with 3×80 ml water and 3×80 ml toluol. The product is crystallized from toluol.

Yield: 9 g

m.p.: 243-246° C.

Further Examples prepared using the same route of synthesis and reaction conditions like described above for Example 46 are the following:

Compounds of formula (II) where m=1, n is 0, the bond between A and N is a single bond and R5 is hydrogen are prepared by the reduction of an intermediate of formula (IV) with hydrogen, e.g. in the presence of a catalyst such as palladium.

6 g of the intermediate A12 are suspended in 200 ml ethanol. 3 ml triethylamine and 3 g palladium catalyst are added. An autoclave is used as reaction vessel. Hydrogen is pressed in up to 20 bar pressure. Now, the mixture is stirred at 70° C. for 4 hours. After filtration the solvent is removed. The crude product is dissolved in 100 ml dichloromethane. This solution is washed with 50 ml water. The solvent is removed to isolate the pure product.

Yield: 4.5 g

m.p.: 169-172° C.

Further Examples prepared using the same route of synthesis and reaction conditions like described above for Example 89 are the following:

Example

R1

R2

R4

R5

m.p. [° C.]

89

—C3H7

—CH3

—H

—H

169-172

90

—C3H7

—H

—OCH3

—H

45-49

91

—C3H7

—CH3

—OCH3

—H

157-160

92

—C3H7

—CH3

—OCH3

—H × HCl

228-231

93

—C2H5

—CH3

—OCH3

—H

139-142

Compounds of formula (II) where m=1, n is 0, the bond between A and N is a single bond and R5 is —C1-5 alkyl are prepared by the treatment of compounds of formula (II) where m=1, n is 0, the bond between A and N is a single bond and R5 is hydrogen with a C1-5alkyl-aldehyde, e.g. in the presence of Raney-Nickel and hydrogen.

1 g 8-methoxy-3-methyl-1-propyl-4,5-dihydro-imidazo[1,5-a]pyrido[3,2-e]pyrazine (Example 91) is suspended in 70 ml methanol. 1 ml methanal and 0.5 g Raney-Nickel are added. An autoclave is used as reaction vessel. Hydrogen is pressed in up to 20 bar pressure. Now, the mixture is stirred at 45° C. for 8 hours. After filtration the solvent is distilled off.

Yield: 0.97 g

m.p.: 113-116° C.

Compounds of formula (II) where m=1, n is 0, the bond between A and N is a single bond and R5 is —(C═O)—C1-5 alkyl are prepared by treatment of compounds of formula (II) where m=1, n is 0, the bond between A and N is a single bond and R5 is hydrogen with alkyl acid chlorides or anhydrides.

To a suspension prepared of 20.0 g KOH (solid), 25.8 g 4-methyl-2-propyl imidazole and 130 ml dimethyl formamide were added 38.0 g 2-chloro-6-methoxy-3-nitro pyridine in small amounts at a reaction temperature of 5° C. The reaction mixture was stirred for 75 minutes at room temperature. Then the reaction mixture was poured in 600 ml water. The mixture was further stirred for 1 hr. The desired product precipitated during this time. The resulting solid was collected by filtration, washed with 100 ml water for 3 times and dried in a dry box with vacuum (40° C.).

To a solution prepared of 138.2 g 6-methoxy-2-(4-methyl-2-propyl-imidazol-1-yl)-3-nitro-pyridine and 900 ml ethyl alcohol 4 g palladium-charcoal were added. The reaction mixture was heated to 40° C. and then hydrogenated under pressure (10 to 15 bar). At room temperature the catalyst was filtrated off and the filtrate was evaporated. To the solid residue 150 ml methyl tert.-butyl ether (MTBE) were added. After stirring for 30 minutes the product was collected by filtration, washed with 50 ml MTBE for 2 times and dried in a dry box with vacuum (40° C.).

A mixture of 20 g 3-amino-6-methoxy-2-(4-methyl-2-propyl-imidazol-1-yl)-pyridine and 60 g urea were heated up to 160° C. The reaction mixture was stirred for 2 hrs. Then 10 ml of glacial acetic acid were added. The stirring was continued for further 6 hrs. The reaction mixture was allowed to cool. At a temperature of 70° C. 300 ml of water were added and the mixture was stirred for 1 hr at 50° C. The warm mixture was filtrated and washed with 50 ml of water for 2 times and dried in a dry box.

A mixture of 27 g 8-methoxy-3-methyl-1-propyl-imidazo[1,5-a]-pyrido[3,2-e]-pyrazinone and 225 ml phosphorus oxychloride were heated to reflux for 8 hrs. To the cooled mixture 250 ml of toluene were added and then 350 ml of the liquid were distilled off. Subsequently the same procedure was performed with 150 ml toluene but 250 ml of the liquid were distilled off. The reaction mixture was allowed to cool at room temperature and then poured in a mixture of 500 g ice/500 ml water. After 30 minutes the mixture was extracted with 250 ml of dichloromethane for two times. The dichloromethane layer was then washed with 500 ml water then with sodium carbonate (3% in water) and after that with 500 ml water. The organic layer was dried with sodium sulfate. After removal of the sodium sulfate and evaporation of the dichloromethane the crude product was dried in a dry box with vacuum (40° C.).

To a solution prepared of 20 g 4-chloro-8-methoxy-3-methyl-1-propyl-imidazo[1,5-a]-pyrido[3,2-e]-pyrazine (Intermediate 3) and 400 ml tetrahydrofuran 80 ml methylmagnesium bromide (3 M in diethyl ether) were added drop wise (via 2 hrs). The reaction mixture was stirred at room temperature for 6 hours. After that the mixture was poured in a mixture of 300 g water, which contained 100 g of ice and 10 g of ammonium chloride. The mixture was extracted for 4 times with 300 ml dichloromethane. The organic layer was separated and then dried with sodium sulfate. After removal of the sodium sulfate and evaporation of the dichloromethane a yellowish-orange crude product remained. This residue was stirred in 150 ml of diethyl ether. After 1 hr. the product was filtrated off and dried in a dry box.

The yield was 11.9 g of crude product (content >95%).

To a solution of 0.05 mol of the crude product and 100 ml of dichloromethane 2.5 equiv. of hydrochloric acid dissolved in 100 ml of water were added. The mixture was vigorously stirred. The dichloromethane layer was then separated and subsequently the water layer was extracted for 6 times with 100 ml dichloromethane. To the organic layer 15 g of sodium carbonate were added. After filtration of the solid precipitate and evaporation of the dichloromethane yellowish crystals remains.

To a solution of 13.52 g of pure 3,4-dimethyl-8-methoxy-1-propyl-imidazo-[1,5-a]-pyrido[3,2-e]-pyrazine and 100 ml of dichloromethane 2.5 equivalents of hydrochloric acid dissolved in 100 ml of water were added. The mixture was vigorously stirred. The dichloromethane layer was then separated and subsequently the water layer was extracted for 6 times with 100 ml dichloromethane. After evaporation of the dichloromethane yellowish crystals remains. (yield 85%; yellowish crystals; m.p. 171-175° C.).

Yield: 13.05 g

m.p.: 171-175° C.

Surprisingly, the compounds of formula (II) are potent inhibitors of the enzyme PDE10. A substance is considered to effectively inhibit PDE10 if it has an IC50 of less than 10 μM, preferably less than 1 μM.

In the prepared brain areas gene segments containing the catalytic domain of the PDE10 were amplified and the sequence determined. Therefore the RNA from the frozen striatum of the different animals was isolated according to the instructions of the RNeasy kit (Qiagen; Hilden; Germany) and transcribed into cDNA using Oligo-Primer provided with the 1st strand cDNA synthese kit for RT-PCR (Roche; Mannheim; Germany). These cDNA was used as template for the PCR-reaction to amplify the catalytic domain of the PDE10. For the PCR reaction Taq-Polymerase (Promega; Mannheim; Germany) was used. Therefore it was possible to clone the amplificates directly by TA-cloning in the pCR2.1 vector (Invitrogen; Karlsruhe; Germany). The cloning vector was transformed into E. coli's (XL-2), replicated within the cells, prepared and the included gene sequence determined for the pig and the guinea pig.

The following primers were used for the PCR-reaction:

P1: tgcatctacagggttaccatggagaa

(SEQ ID NO:1)

P2: tatccctgcaggccttcagcagaggctct

(SEQ ID NO:2)

P3: ttcacatggatatgcgacggtaccttct

(SEQ ID NO:3)

P4: Ctgtgaagaagaactatcggcgggttcctta.

(SEQ ID NO:4)

For the pig the priming was successful with P1 and P2. The following sequence (SEQ ID NO 5) was identified:

tgcatctacagggttaccatggagaagctgtcctaccacagcatttgtac

cgcggaagagtggcaaggcctcatgcgcttcaaccttcccgtccgtcttt

gcaaggagattgaattgttccacttcgacattggtccttttgaaaacatg

tggcctggaatctttgtctatatggttcatcgcttctgtgggacggcctg

ctttgagcttgaaaagctgtgtcgttttatcatgtctgtgaagaagaact

atcgtcgggttccttaccacaactggaagcacgcggtcacggtggcacac

tgcatgtacgccatcctccagaacagccacgggctcttcaccgacctcga

gcgcaaaggactgctaatcgcgtgtctgtgccacgacctggaccacaggg

gcttcagcaacagctacctgcagaaattcgaccaccccctggccgctctc

tactccacgcccaccatggagcagcaccacttctcccagaccgtgtccat

cctccagttggaagggcacaacatcttctccaccctgagctccagtgagt

acgagcaggtgcttgagatcatccgcaaagccatcattgccacagacctc

gctttgtactttggaaacaggaaacagttggaggagatgtaccagaccgg

atcgctaaaccttaataaccagtcacatagagaccgcgtcattggtttga

tgatgactgcctgtgatctctgttccgtgacaaaactgtggccagtaaca

aaactgacggcaaatgatatatatgcggaattctgggccgagggcgatga

ggtgaagaagctgggaatacagcctattcccatgatggacagagacaaga

aggacgaagtcccacaaggccagctcggattctacaacgcggtagctatc

ccctgctacaccaccctcacccagatcttcccgcccacagagcctcttct

gaaggcctgcagggata

For the guinea pig the priming was successful with P4 and P2 as well as for P2 and P3.

The following sequence (SEQ ID NO: 6) was identified with P4 and P2:

ctgtgaagaagaactatcggcgggttccttaccacaactggaagcatgca

gtcacggtggcgcactgcatgtacgccatacttcaaaacaacaatggcct

cttcacagaccttgagcgcaaaggcctgctaattgcctgtctgtgccatg

acctggaccacaggggcttcagtaacagctacctgcagaaattcgaccac

cccctggctgcgttgtactccacctccaccatggagcaacaccacttctc

ccagacggtgttcatcctccagctggaaggacacaacatcttctccaccc

tgagctccagcgagtacgagcaggtgctggagatcatccgcaaagccatc

atcgccactgacctcgcactgtactttgggaacaggaagcagttggagga

gatgtaccagacagggtcgctgaacctcaataaccagtcccatcgagacc

gcgtcatcggcttgatgatgactgcctgcgatctttgctctgtgacgaaa

ctatggccagttacaaaattgacagcaaatgatatatatgcagagttctg

ggctgagggggatgagatgaagaagttggggatacagcccatccctatga

tggacagagacaagaaggatgaagtccctcaaggacagcttggattctac

aatgctgtggccatcccctgctataccaccctgacgcagatcctcccacc

cacagagcctctgctgaaggcctgcagggata

The following sequence (SEQ ID NO: 7) was identified with P2 and P3:

tagagcctctgctgaaggcctgcagggataacctcaatcagtgggagaag

gtaattcgaggggaagagacagcaatgtggatttcaggcccagcaactag

caaaagcacatcagggaagccgaccaggaaggtcgatgactgatcctgag

gtgatgtctgcctagcaactgactcaacctgcttctgtgacttcgttctt

tttatttttatttttttaacggggtgaaaacctctctcagaaggtaccgt

cgcatatccatgtgaa

An alignment of the sequences showed a nearly complete accordance between the rat (published gene-number-NM—022236 3437 bp; coding sequence: 281-2665; catalytic domain 1634-2665) and the guinea pig. More differences were detect between rat and pig. For the alignment the coding areas are used only. The gene alignment is shown in FIG. 3.

This results in the following differences in the protein sequences within the catalytic domain as shown in a protein alignment (FIG. 4).

For the enzymatic testing of PDE10 activity 0.5 g of the isolated and frozen striatum was homogenised in 10 ml 50 mM Tris/Mg-buffer at 4° C. and centrifuged for one hour at 100000 g. The supernatant is called the cytosolic fraction and was removed and stored on ice. The pellet was resuspended in the same buffer, but containing 1% Triton and incubated for 45 min at 4° C. Both fractions were independently applied onto a 5 ml-Hi Trap™ QHP column at the Äkta-FPLC. After washing the columns the bound PDE protein was eluted with an increasing sodium chloride gradient (0 mM-500 mM sodium chloride) in 50 mM Tris/Mg-buffer at 4° C. for the cytosolic fraction and in the presence of 1% Triton for the membrane fraction. The eluted and collected fractions were tested with 100 nM [3H]-cAMP for PDE10-activity in the presence and without a specific PDE-Inhibitor at a concentration, were a 100% inhibition is expected. The fractions with PDE10-activity were pooled and frozen in aliquots until use at −20° C.

The pooled fractions from the FPLC were additional characterized by Western blot. It was shown, that the PDE10A containing pooled fractions include a great number of other cellular proteins. Nevertheless PDE10 was detected with specific antibodies by Western blot clearly (FIG. 1).

The protein was proven in the preparation of the striatum of the rat, the pig and the guinea pig. The main part of protein was found in the membrane fraction (FIG. 2).

Inhibition of PDE10

PDE10 activity was determined in a one step procedure in microtiterplates. The reaction mixture of 100 μl contained 50 mM Tris-HCl/5 mM MgCl2 buffer (pH=7.4) (Sigma, Deisenhofen. Germany; Merck, Darmstadt, Germany) 0.1 μM [3H]-cAMP (Amersham, Buckinghamshire, UK) and the enzyme. Nonspecific activity was tested without the enzyme. The reaction was initiated by addition of the substrate solution and was carried out at 37° C. for 30 minutes. Enzymatic activity was stopped by addition of 25 μl YSi-SPA-beads (Amersham-Pharmacia). One hour later the mixture was measured in a liquid scintillation counter for microtiterplates (Microbeta Trilux). To pipette the incubation mixture a robot Biomek (Fa. Beckman) is used. The determined Km-values for the substrate cAMP is 78 nM for PDE10 from rat striatum, 88 nM for pig striatum and 66.7 nM for guinea pig striatum respectively. cGMP is the second substrate for PDE10, the Km values are 1800 nM, 2200 nM and 1700 nM for PDE10 from these species. For the test with cGMP 500 nM of this substrate was used. The optimal amount of enzyme in the assay has been determined and optimised for each enzyme preparation and substrate separately before using the enzyme in compound testing. For determination of IC50 values the Hill-plot, 2-parameter-model, was used. Specific inhibitors of other PDE-Subtypes do not inhibit the PDE10 preparation significantly. Papaverine was used as the most common PDE10 inhibitor and inhibits the PDE10 with IC50 values of 142 nM, 110 nM and 77 nM for PDE10 from striatum of rat, pig and guinea pig respectively.

Inhibition of PDE10 from rat

Example

IC50 [μM]

35

0.061

38

0.012

62

0.035

63

0.563

69

0.011

70

0.072

91

0.159

95

0.335

Inhibition of PDE10 from pig

Example

IC50 [μM]

1

0.010

29

0.013

30

0.020

31

0.171

35

0.040

38

0.006

39

0.005

40

0.024

41

0.118

42

0.059

43

0.035

44

0.003

45

0.053

46

0.049

47

0.006

48

0.007

49

0.001

52

0.053

53

0.043

54

0.018

55

0.014

57

0.011

58

0.002

59

0.011

60

0.023

62

0.006

63

0.189

65

0.559

66

0.752

67

0.083

68

0.141

69

0.005

71

0.126

72

0.088

73

0.019

75

0.078

79

0.011

80

0.037

84

0.025

85

0.013

86

0.023

87

0.015

91

0.108

95

0.222

Inhibition of PDE10 from guinea pig

Example

IC50 [μM]

29

0.018

30

0.051

38

0.019

47

0.015

58

0.004

62

0.026

69

0.011

The compounds of formula II show significant-antipsychotic effects on the MK-801-induced hyperactivity and stereotyped sniffing, an animal model of psychosis.

Test Procedure:

Female Wistar rats (Crl: (WI) BR, Charles River, Sulzfeld, Germany) weighing 150 to 180 g were used for the MK-801-induced psychosis. Animals were housed under standard conditions in groups of five on a 12 h light/dark cycle (light on at 0600 h) with ad libitum access to food (Pellets, ssniff M/R 15, Spezialdiät GmbH, Soest/Westfalen) and

Compounds were freshly suspended in 0.5% hydroxyethylcellulose so that an administration volume of 0.5 ml/100 g was reached for each substance and dose. Hydroxyethylcellulose was solved in distilled water.

MK-801 was solved in saline so that an administration volume of 0.5 ml/100 g was reached. The suspensions and solution were placed on a magnetic stirrer before and during dosing procedures.

The behaviour induced by the NMDA antagonist MK-801 is generally accepted as a rat model of psychosis. MK-801 induces stereotyped sniffing, hyperactivity and ataxia in rats after intraperitoneal administration.

Locomotor activity of the rats was recorded by the MotiTest Apparatus (TSE, Bad Homburg, Germany). The test area consisted of a squared arena (45×45 cm) with protective plexiglass walls (20 cm of height) where rats could freely move. Horizontal movements were recorded by 32 infrared photocells arranged along the bottom of each wall of the arena. The activity [sec] was measured by the computer program “ActiMot” (TSE, Bad Homburg, Germany).

Stereotyped sniffing was scored by the experimenter every five minutes for one hour (12 intervals) according to the method described by Andiné et al. (1999). The scores of the 12 intervals were summed up at the end of the recording time.

score

stereotyped sniffing

0

no stereotyped sniffing

1

discontinuous sniffing (free interval > 5 s)

2

continuous sniffing

The day of experiment the female rats were placed in the laboratory and received the test compound or vehicle at the appropriate time prior to test. MK-801 0.1 mg/kg was intraperitoneally administered 10 minutes prior to test.

At the beginning of the test the rats were placed in the centre of the squared arena of the MotiTest apparatus. Behaviour of the rats was recorded for one hour. After each run animals were removed and the boxes thoroughly cleaned and dried.

Statistics:

Results were analysed by one way analysis of variance (ANOVA). Tukey test was used for individual comparison. P<0.05 was regarded as significant.

Results:

The results are shown in FIGS. 5, 6, 7 and 8.

FIG. 5 shows the effect of the compounds of Example 91, 35, 95 and 55 on MK-801-induced psychosis

MK-801 at 0.1 mg/kg i.p. was administered 10 min before testing. Compounds at the described doses were administered 30 min prior to the test. Activity and stereotyped sniffing was recorded for 1 h. Cs=control with MK-801 stimulation. Significant to MK-801 stimulated control (=Cs): * p<0.05, *** p<0.001.

FIG. 6 shows the effect of the compounds of Example 38 and 47 on MK-801-induced psychosis

MK-801 at 0.1 mg/kg i.p. was administered 10 min before testing. Compounds at the described doses were administered 30 min prior to the test. Activity and stereotyped sniffing was recorded for 1 h. Co=control without MK-801 stimulation. Cs=control with MK-801 stimulation. Significant to non-stimulated control (Co): ## p<0.01, ### p<0.001. Significant to MK-801 stimulated control (Cs): * p<0.05,** p<0.01, *** p<0.001.

FIG. 7 shows the effect of the compounds of Example 62 and 69 on MK-801-induced psychosis

MK-801 at 0.1 mg/kg i.p. was administered 10 min before testing. Compounds at the described doses were administered 30 min prior to the test. Activity and stereotyped sniffing was recorded for 1 h. Co=control without MK-801 stimulation. Cs=control with MK-801 stimulation. Significant to non-stimulated control (Co): ## p<0.01, ### p<0.001. Significant to MK-801 stimulated control (Cs): * p<0.05, ** p<0.01, *** p<0.001.

FIG. 8 shows the effect of the compounds of Example 29 and 30 on MK-801-induced psychosis

MK-801 at 0.1 mg/kg i.p. was administered 10 min before testing. Compounds at the described doses were administered 30 min prior to the test. Activity and stereotyped sniffing was recorded for 1 h. Co=control without MK-801 stimulation. Cs=control with MK-801 stimulation. Significant to non-stimulated control (Co): ## p<0.01, ### p<0.001. Significant to MK-801 stimulated control (Cs): * p<0.05, *** p<0.001.