Vasculata 2019 Workshops

Vasculata 2019

July 15-18, 2019Milwaukee, WI

Workshops

Workshops will be held on Monday and Tuesday, July 15 and 16 from 1:10pm to 3:00pm

Some workshops are limited to ten attendees unless otherwise noted, please check each workshop. There is an additional fee of $25 for each workshop (Learn what types of skills you need, etc. and Team Science workshops are free)

Workshops will fill up fast - so, register early in order to secure your place.

Workshops A - July 15 from 1:10 to 3:00pm

Drug Discovery

Workshop Leaders:John Imig, PhDProfessor of PharmacologyMedical College of Wisconsin

Blake Hill, PhDProfessor of BiochemistryMedical College of Wisconsin

Description: In this workshop, you will gain exposure to the drug discovery process including chemical library selection and maintenance, screening (in silico and phenotypic), and available therapeutic development programs with pharma and NIH.

Description: Glycans are the predominant molecule on the cell surface and serve as the first point of contact between a cell and other cells, the extracellular matrix and pathogens. The regulatory capacity and structural diversity of glycans arguably surpasses those of other biological molecules. The regulatory capacity stems from the high degree of diversity in linkage, structure and quantity in which glycans can be bound to proteins and lipids to dictating their role as mediators of cellular interaction. Emerging data clearly shows that both structural and quantitative changes in glycans play a role in various disease states including cardiovascular disease, cancer and inflammation. In this workshop, participants will receive a brief didactic introduction to glycan and glycoprotein analysis including 1) blood cell component separation, 2) glycan mass spectrometry and 3) glycan lectin array technology. Participants will also be provided with interactive experiences regarding the fundamentals of sample preparation, data acquisition, and data analysis of N-/O-glycans by lectin array technology and mass spectrometry. Throughout the workshop, participants will learn the advantages and limitations of both approaches to interrogate glycan structures on isolated blood cells.

*We will provide a link to a Box folder.

*This workshop can accommodate only 10 attendees

Manipulating Genomes with CRISPR

Workshop Leader:Aron Geurts, PhDAssociate Professor of Physiology and Genome EngineerDepartment of PhysiologyMedical College of WI

Description: This workshop will be for those who need an introduction to genetic engineering using CRISPR and other tools. It will feature a basic lecture from Dr. Geurts on how these tools work and how they can be applied to generate genetically modified cells and organisms. The lecture will be followed by a demonstrative and/or hands-on exercise (depending on participation) in designing your very own experiment.

Description: Mass spectrometry is a powerful technology for identifying and characterizing proteins and their dynamic changes during development, health, and disease. In this workshop, participants will be introduced to state-of-the-art mass spectrometry and how this technology can be applied to study protein identity, quantity, structure, and post-translational modifications within the context of biomedical research. Hands-on activities in the mass spectrometry laboratory will focus on introducing participants to sample preparation, real-time protein analysis, and data analysis. Throughout the workshop, discussions and examples will focus on specific applications in cardiovascular biology and disease. Participants will need a laptop with Windows operating system and Proteome Discoverer (free, provided by instructors and installed prior to the workshop).

*We will provide a link to a Box folder.

*This workshop can accommodate only 10 attendees

Learn what types of skills you need, and how to develop them, in order to obtain your ideal job.

Description: Have you heard the terms ‘soft skills’, ‘hard skills’, or ‘transferable skills’? Do you know these skills are necessary to obtain a job after your PhD or postdoc training is complete? If not, don’t panic; this workshop can help! This workshop is designed to help you identify the skills required to be competitive in today’s job market. You will learn what skills are most desirable to hiring managers across a spectrum of employment sectors such as academia, industry, government, and for- and not-for-profit industries.

Learning objectives:

(1)Recognize what types of skills are important for success in today’s job market.

(2)Assess your skill levels. Identify which skills you are confident in and which require further developing.

(3)Make a personal plan of action to obtain the skills you need.

(4)Meditate for personal wellness.

Workshops B - July 16, 1:10 to 3:00pm

Big Data Analysis

Description: Catalyzed by the HITECH Act in 2009, health care has become a data-driven industry. Demand for secondary use of Electronic Health Record (EHR) data for business operations, clinical quality initiatives and translational research has risen dramatically and expanded the human and technological resources and processes needed to secure, govern, manage and analyze data. Vasculata 2019’s Big Data Analysis Workshop will help participants understand the opportunities as well as the challenges and complexities that arise with using patient ‘big data’ for research and discovery. A live demonstration of the CTSI’s Clinical Research Data Warehouse toolset will be provided, and audience participation will be encouraged!

There is no limit to the number of attendees

Glycomics and Glycoproteomics

Please see the information above, under Workshop A. This workshop will be offered on Monday, July 15 and Tuesday, July 16. Both presentations are limited to 10 participants each.

Modeling Vascular Disease in Zebrafish

Zebrafish (Danio rerio) have become a valuable animal model to study vascular development and vascular malformations, bridging the gap between cell-culture studies and the more complex mammalian models. Participants in the workshop will be introduced to zebrafish models of human vascular malformations. Topics covered will include introduction to zebrafish husbandry, microinjection of oligonucleotides to affect gene expression, high-resolution imaging of endothelial, erythrocyte, cardiac and inflammatory lineages in live fish, drug discovery and disease modeling. It is hoped that the workshop will equip the participants with a basic understanding of zebrafish and its relevance to vascular research.

There is no limit to the number of attendees

Precision Medicine

Workshop Leader:Michael T. Zimmermann, PhDBioinformatics Research and Development Laboratory, Genomic Sciences and Precision Medicine CenterClinical and Translational Sciences InstituteMedical College of Wisconsin

Description: Precision Medicine is, broadly, the expansion of individualized medical care that leverages new technologies and high-resolution data to define diseases in greater detail and provide tools to researchers and clinicians to more accurately tailor care to each patient. In this workshop, we will give a brief overview of the new technologies for genomic and epigenomic sciences that are already having a positive impact on clinical care. We will then give an overview of the GSPMC interactive RNA-Seq report, which gives investigators an efficient and thorough set of analyses and visualization of their gene expression data, so that they can focus on their hypotheses and identify trends within the data. Finally, we will guide participants through an example of using publicly available RNA-Seq data and web-based tools, in order to explore the data for feasibility studies – how to explore your hypothesis using publicly available data and tools, which could later be tested in analyses such as the GSPMC interactive RNA-Seq report.

*We will provide a link to a Box folder.

Team Science

Description: The “Team Science” workshop will consist of several short talks discussing practical aspects of team science, followed by an extended (30 minutes), interactive panel discussion including questions and comments from the workshop participants. All speakers have experience with NIH program project grants, AHA SFRN or other large team research projects. The Team Science panelists include…

Zeljko Bosnjak, PhdProfessorDepartment of MedicineDivision of EndocrinologyMedical College of WI

Srividya Kidambi, MDInterim Chief, Associate ProfessorDepartment of MedicineDivision of EndocrinologyMedical College of WI