Effects of ethynyl estradiol (EE2) in a multi-generation exposure. F0 and F1 zebrafish were exposed to EE2 (0.5-5 ng/L); a subset of the high concentration F1 was allowed to recover after 50 dpf. Study by Jon Nash1.

The oviducts of most F1 females in the 5 ng/L group contained massive accumulations of egg debris, compared to females from other groups (see control, above). Early and progressed stages of egg resorption can be discerned in this accumulation of debris, which further contains reactive cells (mainly macrophages).
This aberration suggests that these females were unable or at least inhibited to spawn, either physiologically or behaviourally.