You might check out the pET manual at novagen's web site
(www.novagen.com). Since they sell some vectors that direct
expression to the periplasmic space, I do believe they provide a
protocol (and some references) for getting such proteins out.
Mike
>Dear colleagues,
>>Do you know of a protocol to isolate proteins from periplasm and not
>get contamination from cytoplasm?
>>Trond Erik Vee Aune
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