32865EnglishMahapatra, P. S.Jain, S.Shrestha, S.Senapati, S.Puppala, S. P.2017Ambient Endotoxin in Pm10 and Association with Inflammatory Activity, Air Pollutants, and Meteorology, in Chitwan, NepalScience of The Total Environmenthttps://doi.org/10.1016/j.scitotenv.2017.09.249Parth Sarathi Mahapatra, Sujan Shrestha, Siva Praveen Puppala, ICIMOD staff, peerreviewedEndotoxin associated with ambient PM (particulate matter) has been linked to adverse respiratory symptoms, but there have been few studies of ambient endotoxin and its association with co-pollutants and inflammation. Our aim was to measure endotoxin associated with ambient PM10 (particulate matter with aerodynamic diameter<10µm) in summer 2016 at four locations in Chitwan, Nepal, and investigate its association with meteorology, co-pollutants, and inflammatory activity. PM10 concentrations were recorded and filter paper samples were collected using E-samplers; PM1, PM2.5, black carbon (BC), methane (CH4), and carbon monoxide (CO) were also measured. The Limulus amebocyte lysate (LAL) assay was used for endotoxin quantification and the nuclear factor kappa B (NF?B) activation assay to assess inflammatory activity. The mean concentration of PM10 at the different locations ranged from 136 to 189µg/m3, and of endotoxin from 0.29 to 0.53EU/m3. Pollutant presence was positively correlated with endotoxin. Apart from relative humidity, meteorological variations had no significant impact on endotoxin concentration. NF-?B activity was negatively correlated with endotoxin concentration. To the best of our knowledge, this study provides the first measurements of ambient endotoxin associated with PM10 in Nepal. Endotoxin and co-pollutants were positively associated indicating a similar source. Endotoxin was negatively correlated with inflammatory activity as a result of a time-limited forest fire event during the sampling period. Studies of co-pollutants suggested that the higher levels of endotoxin related to biomass burning were accompanied by increased levels of anti-inflammatory agents, which suppressed the endotoxin inflammatory effect.http://www.sciencedirect.com/science/article/pii/S0048969717326001External link (open access)http://lib.icimod.org/record/32865/files/icimod1s2.pdfhttp://lib.icimod.org/record/32865/files/icimod1s2.ps.gzhttp://lib.icimod.org/record/32865/files/icimod1s2.png?subformat=iconiconhttp://lib.icimod.org/record/32865/files/icimod1s2.png?subformat=icon-180icon-180http://lib.icimod.org/record/32865/files/icimod1s2.png?subformat=icon-210icon-210http://lib.icimod.org/record/32865/files/icimod1s2.png?subformat=icon-600icon-600ARTICLE