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This study was conducted to isolate a compound with anticancer properties from the roots of
Peucedanum japonicum
Thunb. (Umbelliferae), and to evaluate the efficacy of that compound’s anticancer activity. The CHCl
3
layer was purified via repeated column chromatography and recrystallization. The two compounds isolated from CHCl
3
layer were identified via NMR spectroscopic analysis as (10E) 1,10-heptadecadiene-4,6-diyne-3,8,9-triol (Comp. I) and anomalin (Comp. II). (10E) 1,10-heptadecadiene-4,6-diyne-3,8,9-triol was the first report from the roots of
P. japonicum
. MTT assays were conducted to evaluate the
in vitro
cytotoxic activities of Compounds I and II against the following human cancer cell lines: HeLa, HepG2, SNU-16, and AGS. Comp. I evidenced the most profound cytotoxic activity against HepG2 cells (IC
50
=6.04㎍/mL), and Comp. II exhibited the most profound cytotoxic activity against SNU-16 cells (IC
50
=18.24㎍/mL) among the human cancer cell lines tested in this study. However, no significant cell death was observed in the CCD-25Lu human normal lung fibroblast cells. Quantitative analysis using UPLC (Ultra Performance Liquid Chromatography) showed that the roots of
P. japonicum
contained 0.015 (Comp. I) and 1.69 ㎍/g (Comp. II) of these compounds.

Traditionally natural products have played an important role in drug discovery. Nature product is an attractive source of new therapeutic candidate compounds. Also, a tremendous chemical diversity has been found in millions of species of plants, animals, marine organisms and microorganisms. Natural products have been invaluable as tools for deciphering the logic of biosynthesis and as platforms for developing frontline drugs (Newman et al., 2000). For example, between 1981 and 2002, 5% of the 1,031 new chemical entities approved as drugs by the US Food and Drug Administration (FDA) were natural products, and another 23% were natural-product-derived molecules. Vincristine, irinotecan, etoposide and paclitaxel are examples of plant-derived compounds that are being employed in cancer treatment (Newman et al., 2003).

Today, millions of people are living with cancer or cancer patients. In Korea, the incidence of cancers is increasing by western diet and low physical activity. Although the disease has therefore existed for at least several thousand years, its prevalence has been steadily increasing. In just the past 50 years, a person’s chance of developing cancer within his or her lifetime has doubled, and doctors are now examining more cases of the disease than ever before. If we live until average life expectancy, the probability of cancer occurrence is 26.1% ( Kushi et al., 2006).

The oldest description of cancer was discovered in Egypt and dates back to approximately 1600 B.C. The term cancer, which means ‘crab’ in Latin, was coined by Hippocrates. Cancer develops when cells in a part of the body begin to grow out of control. Even though there are many kinds of cancer, they all start because of abnormal cells that grow out of control. Normal body cells grow, divide, and die in an orderly fashion. During the early years of a person's life, normal cells divide more rapidly until the person becomes an adult. After that, cells in most parts of the body divide only to replace worn-out or dying cells and to repair injuries. Because cancer cells continue to grow and divide, they are different from normal cells. Cancer cells often travel to other parts of the body where they begin to grow and replace normal tissue (AICA., 2013). The potential of using natural products as anticancer agents was recognized in the 1950s by the U.S. National Cancer Institute (NCI) and has made major contributions to the discovery of new naturally occurring anticancer agents.

Peucedanum japonicum Thunb. is a perennial herb found distributed throughout Korea, Japan, the Philippines, China, and Taiwan. P. japonicum is a perennial plant that grows to a height of 60 ~ 100 cm. The leaves are frequently served as a vegetable or a garnish for raw fish in Korea. The root of the plant is utilized in the treatment of cough, cold, headache, and also as an anodyne (Ikesshiro et al., 1992, 1993). The chemical constituents of P. japonicum have already been studied to some degree, and the khellactone coumarins were identified as the characteristic components. Examinations of the P. japonicum roots have resulted in the isolation of four new khellactone esters and 17 other compounds: isoimperatorin, psoralen, bergapten, xanthotoxol, eugenin, cnidilin, (−)-selinidin, (−)-deltoin, (+)-pteryxin, (+)-peucedanocoumarin III, xanthotoxin, imperatorin, (−)-hamaudol, (+)-visamminol, (+)-marmesin, (+)-oxypeucedanin hydrate and (+)-peucedanol (Chen et al., 1995). Some of the coumarins isolated from P. japonicum have been reported to evidence antiplatelet (Baba et al., 1989; Chen et al., 1996; Hsiao et al., 1998; Jong et al., 1992), antiallergic (Takeuchi et al., 1991), antagonistic, and spasmolytic (Aida et al., 1998) activities. P. japonicum leaf extracts are reported to have strong antioxidant activity (Hisamoto et al., 2002). Moreover, hyuganin C isolated from the stem of P. japonicum has been reported to have anticancer activity (Jang et al., 2008) and it exhibits the highest activity in HL-60 cells (IC50 = 13.2 μg/mL) and A549 cells (IC50 = 18.1 μg/mL). However, the identification of anticancer compounds has never previously been reported from P. japonicum roots.

In this study, we report the isolation of two compounds from P. japonicum roots and their structures as determined via spectroscopic methods. Additionally, the isolated compound was evaluated for its cytotoxic activity against selected cancer cell lines

Materials and Methods

- Plant material

Roots of P. japonicum were collected from wild populations growing on Jeju Island in July.

UV spectra were measured on a Varian Cary100 spectrophotometer. 1H-NMR and 13C-NMR at 500 MHz were obtained with a Bruker AM 500 spectrometer in CDCl3. EIMS was obtained on a JEOLJMS-700 mass spectrometer. TLC was conducted on precoated Kieselgel 60F254 plates (Art. 5715; Merck) and the spots were detected either by examining the plates under a UV lamp or by treating the plates with a 10% ethanolic solution of phosphomolybdic acid (Wako Pure Chemical Industries) followed by heating at 110℃. UPLC was conducted using a Waters US/ACQUITY UPLC Module, Waters US/ACQUITY UPLC Photodiode PDA Detector and BondapakTM C18 column (1.7 M 2.1 × 150 mm) (Waters, Ireland).

The roots of P. japonicum (10 g) were extracted with 100 mL of MeOH overnight in a vortex mixer at room temperature to form the final extract, which was subsequently centrifuged. The extracts employed for UPLC analysis were first passed through a 0.20 ㎛ filter (Advantec MFS, Inc. CA, USA) prior to being injected into a reverse phase μBondapakTM C18. A 20 μL portion of these solutions was injected into the UPLC system. The mobile phase was water containing 0.1% formic acid (A) and acetonitrile (B). A linear gradient of A - B was used (0 min 80 : 20, 2.5 min 80 : 20, 6 min 40 : 60, 8 min 0 : 100, 8.50 min 0 : 100, 9 min 80 : 20 v/v). The flow rate was adjusted to 0.4 mL/min and the detection wavelength was set to 310 nm, while the temperature was held constant at 30℃.

- Standard solution and calibration curves

An external standard method was utilized for quantification. Approximately 5 ~ 10 ㎎ of a standard was dissolved in a 10 mL volumetric flask with MeOH to obtain a stock solution, then stored in a freezer. The working standard solutions were diluted to a series of concentrations with MeOH. The mean areas generated from the standard solutions were plotted against the concentrations to establish the calibration equations.

- Cell viability assay

The effects of the roots of P. japonicum on the viability of a variety of cancer cell lines were determined via an MTT-based assay (Hansan et al., 1989). In brief, exponential-phase cells were collected and transferred to microtiter plates. The cells were then incubated for 72 hours in the presence of various concentrations of P. japonicum root. After incubation, 5 ㎎/mL of MTT solution (Sigma, MO, USA) was added to each well and the cells were incubated for 4 h at 37℃. The plates were then centrifuged for 20 min at 2,500 rpm at room temperature, and the medium was carefully removed. DMSO (150 mL) was then added to each well to dissolve the formazan crystals. The plates were immediately read at 570 nm on a Sunrise microplate reader (Sunrise, Tecan, Salzburg, Austria).

Results and Discussion

The P. japonicum roots were extracted with 100% MeOH. After filtration and concentration the resulting extracts were suspended in H2O and successively partitioned with CHCl3 and n-BuOH, generating CHCl3- and n-BuOH-extractable residues. The two compounds were isolated via repeated chromatographic separation of the CHCl3 fractions, showed cytotoxic activity. The compounds were then structurally identified via the interpretation of several sets of spectral data, and compared with the data described in the literature.

The effects of various concentrations of the (10E) 1,10- heptadecadiene-4,6-diyne-3,8,9-triol (Comp. I) and anomalin (Comp. II) on the growth of HeLa, HepG2, AGS, and SNU-16 cells were evaluated via a MTT assay. The percent viabilities of all cells at 6.25, 12.5, 25, 50, and 100 mg/mL were reduced as compared with the controls. The inhibition of cell growth occurred in a dose-dependent fashion. Quercetin, chrysin, and linoleic acid were reported to evidence anticancer activity in these cell lines. HeLa and HepG2 cells were inhibited by 58% and 60% as the result of treatment with 25 mg/mL of quercetin and chrysin, respectively (Ramos et al., 2008; Zhang, 2004). AGS cells were inhibited by 78% after treatment with 200 mg/mL of linoleic acid (Kwon et al., 2008). Comp. I evidenced the most potent cytotoxic activity against HeLa, HepG2, AGS, and SNU-16 cells. At a concentration of 25 mg/mL, comp. I exhibited inhibitory activities of 69%, 92%, and 78% on HeLa, HepG2, and SNU-16 cells, respectively. Additionally, at a concentration of 100 mg/mL, Comp. I exerted an 82% inhibitory effect on AGS cells (Fig. 5). Comp. II also evidenced profound cytotoxic activity against HeLa and SNU-16 cells. At a concentration of 25 mg/mL, Comp. II exerted inhibitory effects of 63% and 71% on HeLa and SNU-16 cells, respectively (Fig. 5). However, no significant cell death was noted in incubations of CCD25Lu with Comp. I and Comp.

Growth inhibition in cancer cell lines by Comp. I and Comp. II. (A) HeLa cells, plated at a density of 1 × 104 cells/mL, were incubated with various concentrations of Comp. I and Comp. II (6.25-100 μg/mL) for 48 hr; (B) HepG2 cells, plated at density of 1 × 104 cells/mL, were incubated with various concentrations of Comp. I and Comp. II (6.25-100 μg/mL) for 48 hr; (C) AGS cells, plated at density of 1 × 104 cells/mL, were incubated with various concentrations of Comp. I and Comp. II (6.25-100 μg/mL) for 48 hr; (D) SNU-16 cells, plated at a density of 1 × 104 cells/mL, were incubated for 48 h with various concentrations of Comp. I and Comp. II (6.25-100 μg/mL).

II (Fig. 6). According to these results, Comp. I and Comp. II evidenced potent cytotoxic effects against several human cancer cell lines. Although Comp. I and Comp. II had been reported previously to exhibit antibacterial and antifungal properties (Matsuura, 1996), they have never been previously reported to exhibit anticancer activities.

Growth inhibition of CCD-25Lu Human normal lung fibroblast cell by Comp. I and Comp. II. CCD-25Lu cells, plated at density of 1 × 104 cells/mL, were incubated for 48 h with various concentrations of Comp. I and Comp. II (25-200 μg/mL).

The isolated (10E) 1,10-heptadecadiene-4,6-diyne-3,8,9-triol and anomalin were identified via comparisons of the physical and spectroscopic data with those of the authentic standard. They were also subjected to quantitative analysis using UPLC. The representative UPLC chromatogram is shown in Fig. 7. A calibration curve was constructed at different concentrations of (10E) 1,10-heptadecadiene-4,6-diyne-3,8,9-triol and anomalin (6.25, 12.5, 25, 50, 100, and 200 mg /mL) as a standard. The results revealed that the root of P. japonicum contains 0.015 and 1.69 ㎎/g (dry weight) of (10E)1,10-heptadecadiene-4,6- diyne-3,8,9-triol and anomalin, respectively.

This work was supported by “Cooperative Research Program for Agricultural Science & Technology Development (Project No. PJ009470)”, RDA, Republic of Korea, Rural Development Administration, Republic of Korea.