Using SYBR Green and TaqMan Chemistry for Real-Time PCR

Using SYBR® Green andTaqMan® Chemistryfor real-time PCR

Both SYBR® Green and TaqMan® chemistry can be used with real-time PCR analysis to measure gene expression, but they provide different levels of specificity and sensitivity. Watch the videos below and learn more about SYBR® Green and TaqMan® chemistry, and their use in real-time PCR analysis.

In addition, learn how to better understand the results achieved using SYBR® Green chemistry by watching the video below about multiple peaks in the SYBR® Green melt curve. It helps explain how multiple melt curves can impact the interpretation of your real-time PCR results.

How TaqMan chemistry works

Multiple peaks in the SYBR melt curve

Watch the rap battle and learn how to vote for your favorite chemistry