!!Rin Asogi!!!Voiced by: Creator/MamikoNoto (JP), Creator/ColleenClinkenbeard (EN)A badass immortal P.I. working out of Shinjiku.----* AntiHero: She's a good person at heart, but she has a rather icy personality. Plus, when she [[spoiler:closes the door behind Saya to let her get eaten by various, zombie like creatures]], she proves that, despite being on the side of good, she sure as hell won't forgive you for [[MoralEventHorizon crossing the line]].* BackFromTheDead: [[spoiler:Or the closest variant for immortals. Her time spore is removed but thanks to Yggdrasil, her body begins regenerating around it.]]* BadassInANiceSuit: She's a tough-as-nails P.I who dresses in a rather spiffy three-piece suit.* BiTheWay: Being immortal seems to loosen a person sexuality.%%* CryCute%%* DeadpanSnarker* {{Expy}}: Aside from the DistaffCounterpart bit in the YMMV tab, she bears more than a passing resemblence to [[Anime/MobileSuitGundam0083StardustMemory Cima Garahau]].* GoodIsNotNice: Rin is nice, but she won't hesitate to end a bad guys existence in rather wicked ways.* IdentityAmnesia: [[spoiler: After getting mulched by a jet engine and two decades of regeneration, she loses her memory.]]* {{Immortality}}: Through FromASingleCell and being TheUndead.* JekyllAndHyde: Although it's a subversion [[spoiler:when her "bad side" is just Laura impersonating her]].* JerkWithAHeartOfGold: She can be pretty cold and sarcastic, but she's still quite caring towards those close to her.* MsFanservice: Boy howdy is she.* PrivateDetective: How she makes her living.

!!Mimi!!!Voiced by: Creator/RieKugimiya (JP), Creator/JamieMarchi (EN)Rin's sidekick and fellow immortal. Works as a hacker.----* BadassBookworm: An immortal hacker. Kind of a requirement.* BiTheWay: She certainly has a thing for girls after getting a taste for it, but doesn't seem to dislike guys either.* GirlFriday: For Rin.* HardDrinkingPartyGirl: And she even holds her liquor better than Rin.%%* JerkWithAHeartOfGold%%* PlayfulHacker* TheSmartGirl: The tech-savvy sidekick.* TokenMiniMoe: A slight older example but she always looks younger than everyone else she's around.

!!Apos!!!Voiced by: Creator/AkiraIshida (JP), Creator/ToddHaberkorn (EN)A sadistic mother-effer who exists to make Rin's life (And any other persons in his way) an utter misery.----* AGodAmI: Since he [[spoiler: possesses traits of both immortals and angels]], he views himself as the closest thing to a genuine god.* BigBad: Main antagonist of the series.* TheChessmaster: Apos' plan has been in the works for centuries. * {{Expy}}: Of [[Manga/{{Devilman}} Ryo Asuka]].* EvilTastesGood: Just why is he so obsessed with causing as much pain to every immortal he finds? Because their time spores will taste so much ''better'' soaked with the memories of their pain.* FauxAffablyEvil: He'll put up a cheerful facade if he chooses, but it's just an act that hides an utter psycho underneath.* FromASingleCell: Just like Rin.* {{Hermaphrodite}}: [[spoiler:It is unclear what the hell s/he is, but s/he apparently has two sets of genitalia.]]* IHaveYouNowMyProudBeauty: Lord help you if you're an immortal.* KarmicDeath: [[spoiler: He does gain a permanent position with Yggdrasil. [[LaserGuidedKarma By becoming part of it]].]]%%* ManipulativeBastard* {{Sadist}}: One enormous understatement...* SelfMadeOrphan: [[spoiler:Kills his father, Tajimamori]].* SoftSpokenSadist: Rarely raises his voice, even when he has woman bound in chains.

!!Laura!!!Voiced by: Creator/SayakaOhara (JP), Clarine Harp (EN)A gun toting psycho and Rin's arch-nemesis.----* {{Alas Poor Villain}}: Really, near the end of her life, she's been beaten up repeatedly and reduced to Apos' sex slave. It's something of a mercy when she finally dies.* TheDragon: To Apos.* {{Implacable Woman}}* ProfessionalKiller: Her main occupation.* PsychoForHire: ''Very'' psycho.* PsychoLesbian: Again, quite unhinged.* TakingYouWithMe: [[spoiler: A variation. After being crippled, she has Rin insert her Time Spore into Apos so she can torment him with her memories before dying.]]* {{We Can Rebuild H|im}}er: [[spoiler:Apos remakes her as cyborg in [[FoeYay Rin's]] image ([[RapeAsDrama which he proceeds to have some fun with]]) and implants a time spore in her as well.]]

!!Sayara Yamanobe!!!Voiced by: Rie Tanaka (JP), Monica Rial (EN)A scientist behind a number of cruel and terrible experiments----* DepravedBisexual: She's a sexual sadist and rapes both Rin and Kouki in episode 1.* DirtyCoward: After Rin overcomes her zombie hordes and leaves her at their mercy, Sayara cries and begs for her mother.* ImmortalityHurts: Apos grants her immortality sometime after episode 1. However she's preserved in the state she's in, and after being partly eaten by zombies and set on fire, Sayara is permanently stuck that way. She lamented that it took over 20 years for the technology to develop so she could walk again.* MadScientist: At first she dabbles in Cloning (which produced a number of defective pained zombies) and later upgrades to bioterrorism.* {{Sadist}}: She enjoys hurting people, and not just to scientific ends.

!!Tajimamori!!!Voiced by: Creator/JohnSwasey (EN)A mysterious individual with ties to Rin.----* ChekhovsGunman: [[spoiler:A trait he shares with his descendents.]]* HeWhoMustNotBeSeen: We don't meet him until the final episode.* OurAngelsAreDifferent: [[spoiler:The only Angel who has no desire to eat someone. There's a reason for that.]]* StarCrossedLovers: [[spoiler:With Rin.]]* ThereCanOnlyBeOne: [[spoiler:And Apos decides that Tajimamori ain't the One [[SelfMadeOrphan anymore.]]]]* WalkingSpoiler: [[spoiler:His entire existence remains a mystery until the final episode. His status as the Guardian of Yggdrasil is a major plot point that explains Apos' motivations.]]

!!Kouki Maeno!!!Voiced by: Creator/NobuyukiHiyama (JP), Creator/RobertMcCollum (EN)A regular Joe who gets swept into Rin's world.----* AndThenJohnWasAZombie: [[spoiler:To save an immortal, he became an Angel.]] * [[spoiler:BackupFromOtherworld]]: [[spoiler: His spirit helps Rin escape from Yggdrasil in the last episode.]]* [[spoiler:BungledSuicide]]: [[spoiler: Near the end of the first episode he is left with a gun by Rin who tells him that only he can choose what path to take. A gunshot is heard offscreen, suggesting he killed himself. However, he shows up at Rin's office the next day with a bandaged head wound. How he survived shooting himself in the head is never revealed, though it's likely he changed his mind at the last minute as he fired the gun and the bullet only grazed him.]]* {{Deuteragonist}}: Serves as the viewpoint character, when Rin is not it. * CloningBlues: [[spoiler:Though he gets over it after episode 1.]]* [[spoiler:HeroicSacrifice]]: [[spoiler: He consumes a Time Fruit to become an Angel and save Rin from Sayara.]]* [[spoiler:KilledOffForReal: Becomes an Angel.]]* LockedIntoStrangeness: After [[spoiler:attempting suicide and somehow surviving]], he has a permanent spot of miscolored hair.