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We studied an apoptosis phenomenon and its role on neonatal hypoxic-ischemic brain damage (HIE). From our results, we revealed apoptosis was an important role on forming perinatal HIE.The subjects were obtained by the below method. Two-weeks old mice were performed sham-operation and 5-minites ligation of common carotid arteries under ether anesthesia. At one to 14 days after operation, the research materials were removed.The brain tissue were stained with hematoxyline-eosin, nick end-labeling (TUNEL), immunohistochemistry (IHC) and in situ hybrydization (ISH).The results showed the number of apoptosis, which were detected by TUNEL, increased to 7 days after operation. And on the concerning of the protein and mRNA expression of c-fos and bcl-2 family, the peak was 3 days after operation.The furthermore studies are needed to clarify the pathomechanism of neonatal HIE.