This method is applicable for the analysis of Melengesterol acetate (MGA) in bovine fat.

Method Summary

Ground bovine tissue is mixed with anhydrous sodium sulfate before adding hexane:acetone (80,20, v,v). The fat is melted by incubating the mixture in a water bath (at 55 to 60 degrees celcius). The sample is then filtered through a funnel with small silanized
glass wool plug and anhydrous sodium sulphate into a separatory funnel. The sample container is rinsed with hexane and acetonitrile and the contents filtered into the separatory funnel as well. The separatory funnel is stoppered, shaken vigorously and the
bottom layer (acetonitrile) collected into a flask. The hexane layer discarded. However, after evaporating the acetonitrile solution to dryness the residue is reconstituted in hexane and the contents cleanedup by column chromatography (involving florisil and
silanized glass wool). After washing the loaded column, the analyte is eluted with 75,25 hexane,acetone. The hexane:acetone mixture is evaporated to dryness and the extract reconstituted in hexane which is further evaporated to dryness. The final residue is
reconstituted in iso-octane ready for GC-ECD analysis. A capillary analytical column - HP 5 cross-linked PH ME siloxane is used.

Applicable Concentration Range

MGA may be quantified in bovine fat at the concentration level of ≥ 10 µg kg -1.