This line was produced by fusing the human myeloma cell line FU-266, clone E-1 (HAT sensitive, 8-azaguanine resistant and resistant to G-418 - an antibiotic similar to gentamicin) with the murine myeloma P3X63Ag8.653 (see ATCC CRL-1580).

Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 4 x 104 viable cells/mL.Maintain cultures at a cell concentraton between 3 x 104 and 8 x 105 cells/mL.

Medium Renewal: Every 2 to 3 days

Cryopreservation

Complete growth medium supplemented with 7.5% (v/v) DMSO

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37°C

Growth Conditions: The cells are grown in 200 to 400 mcg/mL of G-418 to maintain selection for the human chromosomes.

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.