What concentration of DsiRNAs do I need to use in my experiment to see knockdown?

For an RNAi or antisense experiment, the actual level of target gene knockdown relates to the transfection efficiency. A positive control such as HPRT DsiRNA should always be used in each experiment to assess transfection efficiency.

In addition, varying amounts of test DsiRNA ranging from 0.1 to 50 nM can be used to determine which DsiRNA shows maximum knockdown.