TBE buffer has better buffering capacity then TAE, therefore it can be used for extended and repeated electrophoresis.

Borate from TBE buffer is reported to interact with DNA, which may cause problem in subsequent enzymatic modification of DNA fragment eluted from gel. In such cases, use of TBE buffer should be avoided. TAE is suitable buffer for this purposes.

Optional : One can filter the solution to remove any undissolved materials.

Transfer the solution to autoclavable bottle.Tip(s):
Depending on the consumption, one can make small aliquots of solution.

Sterilize the solution by autocaving (20 minutes at 15 lb/sq.in. (psi) from 121-124°C on liquid cycle).Note(s):
One can sterilize the solution by passing through 0.22 μ filter unit. Filter strilization removes all suspended particles with size more than 0.22 μ which includes most bacteria their spores but not mycoplasma. Morover, it does not inactivate enzyme activities (e.g., DNases). Autoclaving inactivates most enzymes except some (e.g., RNases) and kills most microorgasnisms including mycoplasma.

Storage

Solution can be stored at 15 - 25 °C (room temperature) for several months.