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why would the mycelium in my liquid cultures (5% karo) start growing a little bit and then stop.. and turn brownish? Ive been having issues getting a single LC to work for me... no visible contams, but it simply isnt working for me.

this is how it was for a while.. i swirled the water around and you can see the small little bits of mych in there.. it hasnt grown much since, and then it turned a brownish color (not the water.. the white substance itself turned brown)

Quote:Mych said:why would the mycelium in my liquid cultures (5% karo) start growing a little bit and then stop.. and turn brownish? Ive been having issues getting a single LC to work for me... no visible contams, but it simply isnt working for me.

this is how it was for a while.. i swirled the water around and you can see the small little bits of mych in there.. it hasnt grown much since, and then it turned a brownish color (not the water.. the white substance itself turned brown)

I had similar trouble w/ LC a year ago....

This summer i used 2% Karo Light w/ 2% light(est available) malt extract and it worked fine.

Multispore both times, but different source so I cannot attribute it to a change in medium.

--------------------I'm laughing, but on the inside where you can't hear it.

Quote:Mych said:why would the mycelium in my liquid cultures (5% karo) start growing a little bit and then stop.. and turn brownish? Ive been having issues getting a single LC to work for me... no visible contams, but it simply isnt working for me.

this is how it was for a while.. i swirled the water around and you can see the small little bits of mych in there.. it hasnt grown much since, and then it turned a brownish color (not the water.. the white substance itself turned brown)

I had similar trouble w/ LC a year ago....

This summer i used 2% Karo Light w/ 2% light(est available) malt extract and it worked fine.

Multispore both times, but different source so I cannot attribute it to a change in medium.