that's the thing, i don't want to get them into a hemocytometer--I don't want to lift or count in a hemocytometer.
I want to be able to stain them in the dish so I can just visualize approx number of dead cells, after doing that, I want to stain the cells. I want to be able to distinguish between dead and alive cells.
Do you have any suggestions/protocol of dilution of trypan blue/how long to leave in the dish. have you ever done it before?