In the present study 88 number of corneal swabs were collected from 8 different breeds of dog suffering from corneal diseases. All the dogs were presented to Teaching Veterinary Clinical Complex of Odisha Veterinary College for treatment during the period from December 2018 to June 2019. The breeds consists of Non-descript (n=18), Pug (n=11), Labrador (n=10), German shepherd (n=12), Spitz (n=14), Golden retriever (n=10), Dalmatian (n=7), and Mastiff (n=6). There were no history of injury of the eye prior to infection and all most all the dogs were naturally infected with various microbial agents. In order to identify the microbial isolates and its antimicrobial susceptibility profile all the isolates were subjected to routine microbial procedure. Out of 246 number of bacterial isolates, the most commonly isolated bacteria were Staphylococcus spp. (35%) followed by Streptococcus spp (27%), Pseudomonas spp. (26%), and E. Coli (10%). The antimicrobial susceptibility test was done by disk diffusion method in which ciprofioxacin, cephalexin, neomycin and amoxycillin/clavulanic acid was sensitive to Staphylococcus spp., cephalexin, chloramphenicol and amoxycillin/clavulanic acid was sensitive to Streptococcus spp. and amikacin, gentamicin and tobramycin was found to be highly sensitive to Pseudomonas spp. Similarly, out of 115 fungal isolates, the most commonly fungal isolates were found to be Aspergillus spp. (40%) and Candida spp (59%), whereas mixed fungal infection were found to be prominant. Antifungals like fluconazole, voriconazole and miconazole were found to be sensitive to Aspergillus spp. as well as Candida spp.