The microbial growing is suspected, by inconspicuous hemolytic zones at 24 hours (h) of incubation at 37[degrees]C on bovine blood agar, thereafter at 48 h and with an exhaustive macroscopic examination, it is possible to see white pin point colonies with 1-2 mm diameter, surrounded by a more prominent total ([beta]) hemolysis [9, 46, 57], it is partially acid-alcohol resistant and has a fermentative metabolism [35]; unlike clinical samples, cultures do not have a characteristic odor; CAMP test is helpful to confirm the identification [13,31] and differentiation from Arcanobacterium haemolyticum, which inhibit Staphylococcus aureus [beta] haemolysin [47].

The study also showed significantly higher number of biofilm forming isolates produced haemolysin and coagulase in comparison to planktonic isolates, however biofilm forming isolates were significantly lower producers of protease in comparison to the planktonic isolates.

Dermatophilus congolensis produces virulence factors such as haemolysin, phospholipases, ceramidases and proteolytic enzymes, which may be used to penetrate epidermal barrier and interact with inflammatory response of host (How et.

32] who discovered the differences in haemolysin expression were observed in a strain of Salmonella enterica serovar Typhimurium and Congo red binding activity, On the other hand the result agreed with Chart et al.

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