A bizarre Frankenstein-style experiment has breathed life into the brains of pigs four hours after death. Scientists in the US restored circulation and cellular activity in 32 pig brains obtained from a meat-packing plant. While there was no evidence of reawakening awareness or consciousness, the revitalised brains were said to be "cellularly active". The study has huge implications that challenge long-held assumptions about the finality of death, and opens up a new frontier in brain research. Lead scientist Professor Nenad Sestan, from Yale University, said: "The intact brain of a large mammal retains a previously under-appreciated capacity for restoration of circulation and certain molecular and cellular activities multiple hours after circulatory arrest." Cellular brain death is usually considered to be swift and irreversible. Once the supply of oxygen and blood are cut off, the brain's electrical activity and signs of awareness vanish within seconds. A cascade of destruction then occurs leading to widespread degeneration from which there is no turning back. However, Professor Sestan and his team noticed that small brain tissue samples routinely showed signs of cellular life returning, even when harvested hours after death. To investigate further, they turned their attention to whole, intact brains from pigs processed for food production. In a ground-breaking experiment, a specially designed "chemical blood" preservative was circulated through the brains of dead animals that had been slaughtered four hours earlier. The solution, warmed to normal body temperature, was perfused through the brains' blood vessels for six hours. During this time the scientists observed a reduction in cell death and the restored functionality of certain nerve, blood vessel and glial cells. Glial cells are important brain "support cells" that hold neurons in place, feed them with nutrients and oxygen, provide insulation, and clean up the carcasses of dead cells. The revived activity even included some synaptic function, the transmission of signals between neurons. Details of the study are published in the latest issue of Nature journal. Lessons learned from the study could in future help doctors find ways to salvage brain function in stroke patients, or test the effectiveness of treatments designed to aid cellular recovery after injury, say the scientists. The "BrainEx" system developed at Yale could also provide a powerful research tool. Any future studies involving human tissue or the possible revival of global electrical activity in "dead" animal brains would have to undergo strict ethical supervision, the team stressed. However, it was unclear that the technique would work in a recently deceased human brain. The chemical solution lacked many components found naturally in human blood, such as immune system cells. In Mary Shelley's famous gothic novel, Dr Victor Frankenstein instils life into a monster created from dead body parts obtained from grave yards and butchers. British expert Professor Derek Hill, from University College London, said: "This research raises some fascinating questions. Firstly, was this somehow a chance finding, or can it be reliably replicated? "Secondly, could brains maintained by BrainEx help scientists discover new treatments for brain diseases like Alzheimer's and Parkinson's disease, which are proving really hard to treat? "And thirdly, what are the ethical implications for the way we treat animals after slaughter, and humans after accidents? "This ingenious experimental work provides challenges and opportunities both to brain scientists and for science policy makers." Australian Associated Press

Scientists bring dead pig brains to life

Scientists in the US have managed to reanimate the brains of 32 pigs four hours after they died.

A bizarre Frankenstein-style experiment has breathed life into the brains of pigs four hours after death.

Scientists in the US restored circulation and cellular activity in 32 pig brains obtained from a meat-packing plant.

While there was no evidence of reawakening awareness or consciousness, the revitalised brains were said to be "cellularly active".

The study has huge implications that challenge long-held assumptions about the finality of death, and opens up a new frontier in brain research.

Lead scientist Professor Nenad Sestan, from Yale University, said: "The intact brain of a large mammal retains a previously under-appreciated capacity for restoration of circulation and certain molecular and cellular activities multiple hours after circulatory arrest."

Cellular brain death is usually considered to be swift and irreversible.

Once the supply of oxygen and blood are cut off, the brain's electrical activity and signs of awareness vanish within seconds.

A cascade of destruction then occurs leading to widespread degeneration from which there is no turning back.

However, Professor Sestan and his team noticed that small brain tissue samples routinely showed signs of cellular life returning, even when harvested hours after death.

To investigate further, they turned their attention to whole, intact brains from pigs processed for food production.

In a ground-breaking experiment, a specially designed "chemical blood" preservative was circulated through the brains of dead animals that had been slaughtered four hours earlier.

The solution, warmed to normal body temperature, was perfused through the brains' blood vessels for six hours.

During this time the scientists observed a reduction in cell death and the restored functionality of certain nerve, blood vessel and glial cells.

Glial cells are important brain "support cells" that hold neurons in place, feed them with nutrients and oxygen, provide insulation, and clean up the carcasses of dead cells.

The revived activity even included some synaptic function, the transmission of signals between neurons.

Details of the study are published in the latest issue of Nature journal.

Lessons learned from the study could in future help doctors find ways to salvage brain function in stroke patients, or test the effectiveness of treatments designed to aid cellular recovery after injury, say the scientists.

The "BrainEx" system developed at Yale could also provide a powerful research tool.

Any future studies involving human tissue or the possible revival of global electrical activity in "dead" animal brains would have to undergo strict ethical supervision, the team stressed.

However, it was unclear that the technique would work in a recently deceased human brain.

The chemical solution lacked many components found naturally in human blood, such as immune system cells.

In Mary Shelley's famous gothic novel, Dr Victor Frankenstein instils life into a monster created from dead body parts obtained from grave yards and butchers.

British expert Professor Derek Hill, from University College London, said: "This research raises some fascinating questions. Firstly, was this somehow a chance finding, or can it be reliably replicated?

"Secondly, could brains maintained by BrainEx help scientists discover new treatments for brain diseases like Alzheimer's and Parkinson's disease, which are proving really hard to treat?

"And thirdly, what are the ethical implications for the way we treat animals after slaughter, and humans after accidents?

"This ingenious experimental work provides challenges and opportunities both to brain scientists and for science policy makers."