Abstract

The aim of this study was to investigate the viability and membrane integrity of spotted buffalo epididiymal
sperm. Andromedï was used as control extender and the combination of Andromedï with 0.2% (P1) and
0.4 % (P2) maltose were used for the treatments. The results showed that the percentage of motility after
thawing in P1 media (46.00 ï± 2.00 %) was not significantly different (P>0.05) from P2 (44.00 ï± 2.00 %), but
there was significantly different (P<0.05) from K (41.00 ï± 2.00 %). The percentage of live sperm after
thawing in P1 (59.20 ï± 1.17 %) was not significantly different (P>0.05) from P2 (60.60 ï± 1.62 %), but
significantly different (P<0.05) from K (52.20 ï± 2.48 %). There was no significantly different (P>0.05) in
the percentage of membrane integrity of spotted buffalo epididymal sperm at the three stages of
cryopreservation process in the three different extenders. In conclusion, the addition of maltose into
Andromedï could maintain the viability and membrane integrity of spotted buffalo epididymal sperm after
thawing.
Keywords: Viability, Plasma membrane, Epididymal sperm, Maltose, Spotted buffalo