[Histonet] Fixed IF's, small brittle bx's

From:

JColCLEFA@aol.com

George, the absolutes, much like absolute alcohol would if left open, have
certainly evaporated. Borrowing from my total formalin fixation technique IHC
lab, I have successfully and routinely stained skin biopsies as well as kidney
biopsies with FITC conjugated antibodies after enzyme pretreatment with
Protease 0.1%, 30 minutes@ 37C. The kidneys were fixed in Zamboni's fixative, the
skin in Formalin, both paraffin processed. The only "artefact" was a
reduction in non-specific background staining. ( a plus in my book!) In the kidney
arena, we now prefer fixed tissue to fresh frozen. J Coleman HT(ASCP)QIHC
we have found that overdehydration of small biopsies is the culprit.
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