>I have been screening phage lifts on NC filters with kinased oligos and have
>been getting a lot of "polka dot" background. Does any one know what causes
>this and how to avoid it. I never get this with RNA or DNA probes.
>> Condie E. Carmack ====================================================
> GenPharm International ====================================================
>297 North Bernardo Avenue \/
> Mountain View, CA 94043 ================= =================================
> (415) 988-2434 ===================================================
Hi
My guess would be excessively high levels of unincorporated nucleotides.
Do you check your level of incorporation?? I have a rapid method for
radiolabelled probes using a PEI tlc plate if you would like.
Cheers, Klaus
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Klaus Matthaei
Gene Targeting
The John Curtin School of Medical Research
The Australian National University
E-mail: Klaus.Matthaei at anu.edu.au
"If you don't do: you rust"
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