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Flower production, which inhibits root growth, can be circumvented by the culture of isolated rooted leaves. The absolute amount of alkaloid in such leaves can be as much as a hundred times that in the leaves on the plant and twenty times as much fractionally when based on dry weight (286). As has already been pointed out there is a positive and recognizable relation between amino acids and alkaloids, but we have searched in vain for one between proteins and alkaloids (250, 444). When tryptophan was injected into the internodes of rye infected with ergot there was a slight increase in the ergot alkaloids.