The C6 cell line was established from the brain tissue of a rat (Rattus norvegicus) glial tumor. This strain of cells was isolated by N-nitrosomethylurea by Benda et al. These cells exhibit fibrobast morphology, and come from the rattus Rattus norvegicus (rat). This cell line could be useful for research related to brain tumors found in mammals. C6 cells have been known to exhibit glucocorticoid receptors, and they also make for a good transfection research model.

Data:

Figure 1. siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into C6 cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for Lamin A/C gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=4).

Figure 2. Protein expression of Lamin A/C in C6 cells. DNA plasmid expressing Lamin A/C or siRNA targeting Lamin A/C were transfected into C6 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.