An easier way on Windows XP and above if you have a mixture of USB 1.1 and 2.0 ports is to just plug in a USB 2.0 compliant device. If the port is USB 1.1, Windows will notify you that moving to a faster port will improve performance. If you don't receive a notification, its safe to assume the port is USB 2.0 compliant.
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Kenneth CochranJul 28 '09 at 13:25

I have heard somewhere that there can be both 1.1 and 2.0 controllers in a single chip, selecting the right one automatically. Dunno if that makes any sense though.
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grawityJan 19 '10 at 15:57

@Kenneth, that just turns the question into "how do I know if [some device] is USB 2.0-compliant?" Good tip though.
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Pops♦Jul 11 '10 at 22:42

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BTW, on Device Manager, the "View" / "Devices by Connection" is immensely more useful for tracking like this than the "Devices by Type", because then you can see the connection path for every device. Like, "Keyboard connects to USB hub X, which connects to hub Y".
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ZdsAug 16 '11 at 16:50

On Mac OS X it depends on the hardware, not the software. If you are running a fairly modern Mac, then you have USB 2.0. All Intel Macs and the last couple of generations of PowerPC machines have USB 2.0. I had a first generation aluminum PowerBook 17" that I bought 5.5 years ago. That machine had USB 2.0, so unless you get a really old machine it will probably have USB 2.0.