transformation of meningococci

Dr. Kris Partridge wrote:
>> I'm about to try to produce meningococci that express a foreign
> protein. Does anyone know if meningococci will accept a plasmid? To
> the best of my knowledge, I've seen nothing in the liturature.
>> Thanks for your help,
>> Kris Partridge
Contact Dr.Xavier Nassif at: nassif at necker.fr
He's built a series of shuttle vectors (the pMGC series) with a variety
of features. They can be mated into gonococci or meningococci. These
are derived from pLES2 (Stein & al., 1983, Gene 25:241). The most
useful pMGC vectors for your purposes are probably MGC10 (ErmR, with
blue/white selection in E. coli) and 18.1 (KmR, smaller size, no
blue/white).
For mating proceedures try:Seifert & So, _Methods in Enzymology_, 204:342.
We have these vectors too, but Xavier actually made them.
I'll warn you in advance: they are finicky, and it can be hard to
work with them even in E. coli.
Alternatively, it's really easy to drop things into the Neisserial
chromosome - ideas on how to do this are suggested in the Seifert/So
article. Best of luck...
..........................................................................
Alexey Merz | URL: http://www.webcom.com/alexey | email: alexey at webcom.com
| PGP public key: http://pgp5.ai.mit.edu/ | voice:503/494-6840
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