Add 200 µl ethanol to the sample, and mix by pulse-vortexing for 15 s.

Carefully apply the mixture from step 4 (including the precipitate) to the Spin Column (in a 2 ml collection tube). Close the cap, and centrifuge at 6000 x g (8000 rpm) for 1 min. Place the Spin Column in a clean 2 ml collection tube (provided),and discard the tube containing the filtrate.

Carefully open the Spin Column and add 500 µl Buffer AW1. Close the cap, and centrifuge at 6000 x g (8000 rpm) for 1 min.

Place the SpinColumn in a clean 2 ml collection tube and discard the collection tube containing the filtrate.

Carefully open the Spin Column and add 500 µl Buffer AW2 without wetting the rim. Close the cap and centrifuge at full speed (14,000 rpm) for 3 min.

Place the SpinColumn in a clean 2 ml collection tube and discard the collection tube containing the filtrate

Centrifuge at full speed (14,000 rpm) for 2 min.

Place the SpinColumn in a clean 1.5 ml tube and discard the collection tube

Carefully open the Spin Column and add 100 µl Buffer AE. Incubate at room temperature for 2 min, and then centrifuge at 12 000 rpm for 1 min. Repeat this step.

Your DNA sample is now in the 1.5ml tube (should be 200ul). Discard the Spin Column.