Hi, netters:
I have a question about a cDNA library screen. I don't know why there
are a few big round(2-4 mm diameter) clear plaques among those normal
size plaques in the plates. I tried both SURE and XL1-blue as a host,
Uni-ZAP XR phage as a vecter. In 150 mm plate I added both 25,000 and
50,000 pfu with 600 ul host which O.D.600 = 0.5. I did a few times
and can't get rid of those big clear plaqures. I still wonder wether
these big plaques have any affect to my screening and why it happen?
Dose anyone out there have these experience? Any suggestion is
welcome.
Thank you for your attention.
Suisheng Tang
suisheng at sfu.ca