Datasheet Extract

Western blot: 2 µg/ml.Immunohistochemistry on Frozen and Paraffin Sections: 1-10 µg/ml, Paraffin sections may require heat mediated antigen retrieval with 10mM citrate buffer pH 6.0; According to customer information this antibody can be used on decalcified tissue pretreated with 0.2% hyaluronidase in sodium acetate buffer for 1h at 37°C.

Background

Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in the gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.