Description

Specifications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Applications

ELISA

Western blot

SDS-PAGE

FormLyophilised

Concentration information loading...

Preparation and Storage

Stability and Storage

Shipped at 4°C. Store at +4°C.

Preservative: None
Constituents: PBS

ReconstitutionReconstitute with deionized H2O.

General Info

Alternative names

Major capsid protein VP1

Major structural protein VP1

RelevanceSimian virus 40 (SV40) is a small, non enveloped DNA virus with an icosahedral capsid of 45 nm. The simian virus 40 capsid is composed of 72 pentamers of VP1, the major protein of SV40. These pentamers are arranged in a T=7d icosahedral surface lattice, which is maintained by three types of appropriately arranged, non-equivalent interactions between the pentamers.
Simian Virus 40 Major Capsid VP1 binds to N-glycolylneuraminic analog of the ganglioside GM1 on the cell surface to provide virion attachment to target cell. Once attached, the virion enters a caveolae and traffics to the endoplasmic reticulum. Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating. Next, the virion uses the endoplasmic reticulum-associated degradation machinery to probably translocate in the cytosol before reaching the nucleus. The assembly takes place in the cell nucleus, encapsulates the genomic DNA and participates in rearranging nucleosomes around the viral DNA. The viral progenies exit the cells by lytic release.

Thank you for your reply. I can confirm that the antibody ab34756 will react with the SV40 VP1 protein ab74565. On the antibody datasheet in the section labelled "cross-reactivity", it notes that it does react with the VP1 protein of SV40. I hope this...

Thank you for your call this week and for your patience while I have been in touch with the production lab about these proteins. I have confirmed with the lab that these proteins do not contain any tag. To detect them, you could use specific monoclonal...