Representation oligonucleotide microarray analysis

Representational oligonucleotide microarray analysis (ROMA) is a technique that was developed by Michael Wigler and Rob Lucito at the Cold Spring Harbor Laboratory (CSHL) in 2003. Wigler and Lucito currently run laboratories at CSHL using ROMA to explore genomic copy number variation in cancer and other genetic diseases.

In this technique, two genomes are compared for their differences in copy number on a microarray. The ROMA technology emerged from a previous method called Representational Difference Analysis (RDA). ROMA, in comparison to other comparative genomic hybridization (CGH) techniques, has the advantage of reducing the complexity of a genome with a restriction enzyme which highly increases the efficiency of genomic fragment hybridization to a microarray.

In ROMA, a genome is digested with a restriction enzyme, ligated with adapters specific to the restriction fragment sticky ends and amplified by PCR. After the PCR step, representations of the entire genome (restriction fragments) are amplified to pronounce relative increases, decreases or preserve equal copy number in the two genomes. The representations of the two different genomes are labeled with different fluorophores and co-hybridized to a microarray with probes specific to locations across the entire human genome. After analysis of the ROMA microarray image is completed, a copy number profile of the entire human genome is generated. This allows researchers to detect with high accuracy amplifications (amplicons) and deletions that occur across the entire genome.

David Rossi

David Stephen Rossi is a fictional character in the CBS crime drama Criminal Minds, portrayed by Joe Mantegna. He is a Supervisory Special Agent of the FBI's Behavioral Analysis Unit, and has appeared from the episode "About Face", which was originally broadcast on October 31, 2007, during the show's third season.

Rossi begins the series returning to the FBI after a lengthy period of being semi-retired, with his return due to "unfinished business". He is shown to be emotionally tied to Aaron Hotchner, as they both worked together during the early days of the Behaviorial Analysis Unit. Rossi is also a writer, and is one of the team's senior and most decorated profilers. He replaced Jason Gideon, who was written out following Mandy Patinkin's abrupt departure from the series.

Mantegna has stated in an interview that the character was named after one of the policemen who had testified at the O. J. Simpson trial. Rossi's middle name, Stephen, was revealed in a flashback near the end of the tenth season episode, "Nelson's Sparrow." After Rossi has deduced that Jason Gideon is about to become a father, Gideon asks Rossi what his middle name is. This is followed by a present-time scene where Rossi greets Gideon's son, Stephen, by name. (The "ph" spelling of the name was used in the episode's end credits.)

History

The channel is the direct descendant of the first radio station to operate in Italy, which began its transmissions from Rome on 6 October 1924. This station, call sign 1-RO, was operated by the privately owned Unione Radiofonica Italiana (URI). In 1927 this company was absorbed by the state-owned Ente Italiano per le Audizioni Radiofoniche (EIAR) which from that date became the only authorized radio broadcaster in Italy.

The EIAR was renamed Radio Audizioni Italiane (RAI) in 1944 and as part of the reconstruction and improvement of the surviving transmitter network following World War II, radio broadcasting was reorganized (with effect from 3 November 1946) to provide two national channels covering most of the country. The first channel was known as the Rete Rossa (red network) and the second as the Rete Azzurra (blue network). These "neutral" names were chosen to imply that, while at any one time each channel aimed to provide programming of a contrasting style to that available on the other, the two channels were nominally equal in status and had an equally wide-ranging remit.

RadioCityIndia’s first & premium web radio touched the hearts of their Bhojpuri listeners across the world with the launch of its 52nd web radio station , Radio City Love Guru Bhojpuri, a dedicated web radio station that doles out love advice in Bhojpuri which was launched by Bhojpuri superstar Ravi Kishan...Radio City Love Guru has been a marquee property of Radio City, since the station’s inception....

Latest News for: roma radio

RadioCityIndia’s first & premium web radio touched the hearts of their Bhojpuri listeners across the world with the launch of its 52nd web radio station , Radio City Love Guru Bhojpuri, a dedicated web radio station that doles out love advice in Bhojpuri which was launched by Bhojpuri superstar Ravi Kishan...Radio City Love Guru has been a marquee property of Radio City, since the station’s inception....

Temperatures are expected to once again rise into the mid 90s this week in north Texas for the 2018 Fort Worth Invitational, but the sweltering field will at least be a good one ...Open in three weeks ... Round starts. 8 a.m. Featured groups. 8 a.m ... Radio ... on PGA Tour RadioRounds3-4 -- Friday and Saturday ... Radio ... on PGA Tour Radio. ....

The BTS boys are taking over the world (Picture. BACKGRID). RomanKemp has found his opinions might not be shared by other radio presenters on the topic of K-Pop. The Capital FM presenter reckons K-pop will work its way onto the airwaves in the UK eventually – although doesn’t think that time has come just yet. However Radio 1 presenters Nikki and Sammy do not agree. No, not even a little bit. Did we hear you say ‘Army’?! ... Advertisement ... ....

Meghan Trainor is set to perform at the 2018 RadioDisneyMusic Awards ... This is Trainor's first time performing at the Radio Disney Music Awards, which airs Saturday, June 23, at 8 p.m. ET on Disney Channel ... ....

radio noise. I say "most people," because radio astronomers actually think about radio noise a lot. Like optical astronomers, who need dark skies to pick out the faint light of distant stars and galaxies, radio astronomers need radio-quiet skies to "listen" to cosmic objects that give off radio waves ... But astronomers taking data across wider swaths of the radio band have to surgically excise interference from their observations....