PRICE LIST
There have been no price rises for NCPPB cultures for several years. Due to increased costs,
especially for transport of cultures and to meet the needs of new legislation, we have had to
increase our prices.

Prices from 1 January 2015

Ampoule of freeze dried culture

£75.00 each

Packaging, postage and handling charge for UK delivery

£30.00 per order

Packaging, postage and handling charge for overseas delivery

£50.00 per order

Application for export licence for Australia group organisms for export outside the EU

£POA

DNA Extracts

DNA extracts can be prepared from NCPPB cultures if preferred.

Prices:

Price per extract

£95 + shipping

Shipping UK = £30

Shipping overseas = £50

An Export Licence for DNA from
Australia List
organisms may be required. See above

Fatty Acid Profiling

This method is UKAS accredited and can be regarded as a good method for rapid, accurate and
inexpensive identification of bacteria to genus, species or sub-specific rank. Our FAP system
is based on the Sherlock Microbial Identification System by MIDI Inc. We require that strains
to be identified by FAP are supplied as pure cultures on a simple solid media, such as Nutrient
Agar, or on Trypticase Soy Broth Agar (TSBA).

Prices:

1 isolate

£123.00

2 to 5 isolates

£75.00 per isolate

6 to 10 isolates

£41.00 per isolate

11 to 20 isolates

£32.00 per isolate

21 or more isolates

£27.00 per isolate

*Fatty acid profiling alone cannot always be relied upon to provide a definitive identification;
further tests may be required and we are able to discuss the most appropriate course of action.

DNA sequencing

Comparative DNA sequencing analysis is widely considered to be the best genotypic method for microbial
identification to species level for many bacteria. The most common approach is PCR amplification and
sequencing of all or a 500bp portion of the 16S ribosomal RNA gene. Sequence data is then compared against
a sequence database. We can also sequence 'House Keeping' genes such as gyrB, rpoD, and recA. For example,
gyrB sequences are sufficiently distinct to allow discrimination of the majority of established species in the
genus Xanthomonas. The gyrB phylogeny offers greater resolution amongst Xanthomonads than those produced
previously from the 16S rRNA gene or 16S-23S intergenic loci. We would recommend using rpoD for plant
pathogenic Pseudomonads and recA for the plant pathogenic Enterobacteriaciae