Abstract

The genetics of Drosophila is a powerful tool in the analysis of mutants and mutant proteins. Cultures of cells derived from wild‐type or mutant flies
can be pulse labeled to biosynthetically label the proteins made by the cells. Immunoprecipitation (and subcellular fractionation)
are used to characterize the expression of specific proteins.

CAUTION: When working with radioactive materials, take appropriate precautions to avoid contamination of the experimenter and the
surroundings. Carry out the experiment and dispose of wastes in an appropriately designated area, following guidelines provided
by the local radiation safety officer (also see unit7.1 and appendix 1D).

An excellent and comprehensive description of Drosophila cell culture protocols that includes methods for generating both primary and continuous cell lines, media formulations, and experimental uses of Drosophila cells.