HomeFAQsWhat is the maximum number of nucleotides that can be inserted with this kit?

FAQ: What is the maximum number of nucleotides that can be inserted with this kit?

Due to the back-to-back primer design, insertions up to 100 nucleotides can routinely be tolerated by appending up to 50 nucleotides to the 5’ end of each primer. Note that HPLC or PAGE purification is recommended for any primer greater than 60 nts.