A LavenderAnything Muppet portrayed Alice in an interview with Kermit the Frog as part of a Sesame Street News Flash segment. Although she is almost too tiny to be seen at first, Alice grows taller upon drinking from a water bottle labeled "Drink Me," eventually becoming as tall as Kermit and then outgrowing him until she is so huge that all that is seen of her is her red and white striped stocking.

The White Rabbit

Alice follows The White Rabbit down the rabbit hole into Wonderland. Alice encounters him again when he mistakes her for his housemaid Mary Ann and she becomes trapped in his house after growing too large. The Rabbit shows up again in the last few chapters, as a herald-like servant of the King and Queen of Hearts.

The White Rabbit appeared in episode 506 as one of the only original puppets in the Muppets' adaptation. Somewhat more aggressive than most versions of the character, the White Rabbit at one point bodily threatens Kermit. He is featured prominently throughout the episode, participating in the "When You're Smiling" medley and later playing the usher in the courtroom scene.

As in the original Carroll novel, the White Rabbit is perpetually concerned about running late, mistakes Alice for his maid, and functions as clerk of the court for the Knave's trial. In this adaptation, the White Rabbit is a full-bodied character with rather twitchy body movements, suggestive of a clockwork figure. According to a pop-up book which leads to the rabbit's house, his full name is Frederick Rabbit.

Manifesting in Alice Hargreaves' hotel room along with the Dormouse and March Hare, the Mad Hatter is more menacing and grotesque than his print counterpart in Lewis Carroll's Alice's Adventures in Wonderland. Trembling in a manner reminiscent of mercury poisoning (also known as "hatter's shakes"), he beats his companions and browbeats the elderly Alice, shouting "You should be dead!" He soon returns to musing about his slow watch, repaired with the best butter.

The March Hare

In Alice's Adventures in Wonderland, the March Hare hosts Alice, the Mad Hatter, and the Dormouse at a Mad Tea-Party. He also later appears during the Mad Hatter's testimony at Alice's trial. In Through the Looking-Glass, he makes a cameo appearance as "Haigha," one of the White King's messengers at the fight between the Lion and the Unicorn.

A Muppet version of the March Hare appears in episode 506. The puppet was designed to resemble the original illustrations by John Tenniel. The March Hare appears several times throughout the episode, in the background or chorus, and is featured more prominently in the Mad Tea-Party scene at the end of the show.

A more feral version of the March Hare character appears in Alice Hargreaves' nightmares. With enraged eyes and blood-stained teeth, the Hare sits with the Mad Hatter and the Dormouse. The group reenact their literary scenes while simultaneously tormenting the elderly Alice. Despite his grotesque appearance, the March Hare is himself cowed by the Hatter, who turns upon him violently.

Bert comments on their brief scene as the twin characters. Ernie explains they were lucky to make it into the production at all, as it's a common misconception that Tweedledum and Tweedledee appear in the original story Alice's Adventures in Wonderland (the characters first appeared in the sequel, Through the Looking-Glass).

The Mock Turtle

The Mock Turtle, along with the Gryphon, are the first Wonderland characters encountered in the dreams and imaginations of the now elderly Alice Hargreaves. The perpetually sobbing turtle symbolizes the Rev. Charles Dodgson, who stutteringly performs the character's song to a young adult Alice's derision.

The Gryphon

The Gryphon, along with the Mock Turtle, are the first Wonderland characters encountered in the dreams and imaginations of the now elderly Alice Hargreaves. The towering, harsh gryphon speaks in a Scots accent and mocks his companion's sorrows, saying it's all his fancy.

Flower

Alice encounters Tiger Lily, a talking flower. She asserts that the reason garden flowers seldom speak is because the flowerbeds are too soft, sending the plants to sleep. She attempts to assert her authority over the other plants, but they know she can't get at them.

A querulous, somewhat grotesque infant, the baby apparently belongs to The Duchess, where he's shaken and subjected to the effects of pepper and The Cook's tantrums. Alice attempts to rescue the baby, but the moment they get out of the house, he turns into a live pig.