Culturing hESCs on Corning Synthemax Vitronectin

Culturing hESCs on Corning Synthemax Vitronectin

Culturing hESCs on Corning Synthemax Vitronectin Substrate

Current methods to maintain hESCs in an undifferentiated state often employ mouse feeder layers or biological substrates, but these methods can be difficult to work with, inconsistent and can introduce the possibility of contamination from the animal source. This protocol describes maintenance of human embryonic stem cells H1 and H7 WiCell® lines on Synthemax vitronectin substrate using a chemically defined medium.