Table 1 shows the resulting change in height of the liquid solution when catalase and hydrogen peroxide reacted in various levels of pH solution. Twenty trials were completed to calculate a class average, including trial 18 which was data collected by my partner and me. During the first trial there was a minimal reaction. The reaction became more intense until reaching pH 7. The higher pH levels reaction was not as dramatic.

Figure 1 shows height of the liquid solution when catalase (the enzyme) and hydrogen peroxide (the substrate) reacted at various levels of pH. My specific trial data is compared to the class average data, consisting of twenty total trials.

The hypothesis of the experiment was that the test tube with the most basic pH would produce the most product (overall solution). This hypothesis was refuted. The data as shown in Figure 1 indicates that the most active reaction (highest change in level of solution produced) came from the test tube with a pH level of seven, followed by pH level five and pH level nine, which means that the enzyme catalase works most effectively at a neutral pH level. (Figure 1)
When the pH was varied too dramatically from neutral, the active site on the protein structure was denatured. This means that it was altered, stopping the catalyse from breaking up the H2O2 efficiently. When the solution was too acidic (low pH value of 3) or too basic (high pH value of 11), the catalase was less active.
This is why the cells inside our bodies should be neutral (pH 7). Human bodies create hydrogen peroxide during normal metabolic events and catalase quickly breaks it down into H2O and O2 before it becomes toxic (Nowicki, 2012).

For this particular experiment it was very important that the test tubes have the exact same amount of solution at the start so that final solution could be measured accurately. A few other errors that could have skewed the results were that the amount of enzymes added may not have been exactly equal or contamination of the test tubes or reactants may have adversely affected the reaction in addition the first ten trials were taken at a different time that the last ten which means the temperature may have been different (N.A. 2012).
If the pH level in our cells were to become too basic or too acidic, the cell’s DNA and other proteins can become damaged. Scientists think this might be a cause of cancer, diabetes, and other serious aliments (N.A. 2012).

Background:
Catalysts speed up chemical reactions by lowering activation energy, the energy needed for a reaction to begin. In every chemical reaction, the starting materials can take many different paths to forming products (N.A. 2011). For each path, there is an intermediate or transitional product between reactants and…

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each…
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Weak Acid/base:
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1) 2) 3) 4)
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1) 2) 3) 4)
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observe the activity of an enzyme in a tissue extract using a spectrophotometer, and to test whether the amount of the enzyme influences the rate of the reaction, whether the temperature of the solution influences the activity of an enzyme, or whether the pH of the solution influences the activity of the enzyme. Thousands of chemical reactions occur in a cell each minute, these reactions are not random events but are controlled by biological catalysts called enzymes. Like all catalysts, enzymes lower the…

Effect of Catalase on Hydrogen Peroxide
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Metabolism is the sum total of chemical reactions in the body that are necessary to the maintenance of life.
Enzymes are biological catalysts that can speed up, and control, chemical reactions that would otherwise virtually
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equation which causes the breakdown of Hydrogen Peroxide into water and oxygen:
Hydrogen Peroxide Water + Oxygen
2H2O2 H2O + O2
When we immerse small discs of filter paper into the yeast suspensions and then drop them into our solution of hydrogen peroxide, a reaction occurs. This reaction involves bubbles being produced that are attached to the filter discs and these bubbles force them to rise to the surface of the solution.
Aim:
To test that pH levels affect the activity of catalase…

are folded in order to create the active site.
All enzymes have five important properties;
They are all proteins
Each enzyme controls one particular reaction
They can be used again and again
They are affected by temperature
They are affected by pH
Looking at the first point 'They are all proteins', when you heat a protein, it changes shape. For example; hair. Hair is a protein, and when you heat hair, it changes shape. This is called denaturing. Therefore, as enzymes are proteins, when heated…

Independent Variables
* Concentration of substrate – different concentrations of the substrate can have an effect on the rate of reaction due to more particles in the solution therefore increasing chance of the particles colliding.
* pH – different pH levels can have an effect on the rate of reaction due to the interference of bonds that hold the enzyme together in the active site. Therefore the specific substrate will not fit into the active site
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Does The Concentration Of Hydrogen Peroxide Affect The Time Taken For A Bead To Lift Off?
Data Collection and Processing & Conclusion and Evaluation
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Data Collection and Processing
Qualitative Data:
When dropping the bead into the different concentrations the beads slowly sank to the bottom, bubbles formed around the bead before it took off.
As the concentration of hydrogen peroxide increased the time that the bead took to rise decreased.
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Common Foods and the pH Scale
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Acids and Bases
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The Arrhenius definition of acids says that they’re compounds that give off H+ ions in water and that bases are compounds that give off OH- ions in water.
These definitions are the same. Basically…

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