Working in Cytobank with data compensated in a different software

In most Cytometry software, data are written in uncompensated format and the compensation matrix is included within the files alongside the data. This internal instruction for compensation is called a "file-internal compensation" and is typically held in the FCS variables of SPILL or $SPILLOVER. In Cytobank, use the file-internal option whenever choosing a compensation to have your data automatically compensated on a per-file basis according to the internal instructions. Data can still be viewed as uncompensated by changing the compensation selector to "uncompensated"