Sequencing of proteins and peptides

The amino acid sequence analysis offered by Selvita is performed by chemical sequencing (Edman degradation) or by MS method (peptide mapping and LC-ESI MS/MS sequencing). Determination of amino acid sequence of the entire protein by chemical sequencing is more reliable than MS method, however due to the relatively high cost and time requirements, this analysis is limited to proteins composed of close to 300 aa. For larger proteins the MS sequencing method is generally recommended.