Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Introduction

Cell Line Description:

HCC1937BL is a B lymphoblastoid cell line initiated by Epstein-Barr virus (EBV) transformation of peripheral blood lymphocytes obtained from the same patient as HCC1937 (a primary breast ductal carcinoma cell line, ATCC CRL-2336). BRCA1 analysis revealed that this cell line is heterozygous for the BRCA1 5382C mutation, whereas the primary tumor cell line is homozygous for the same mutation. DNA isolated from the cell line HCC1937 is available as CRL-2336D.

Quality Control Specifications

DNA Quality Control Information:

· > 100 kb in size by agarose gel electrophoresis

· No RNA detected in the agarose gel

· A260/A280 ratio ³ 1.8

· Tested and verified for PCR amplification and restriction digestion.

Permits

These permits may be required for shipping this product:

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.