How to optimize the performance and reliability of qPCR analyses

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How to optimize the performance and reliability of qPCR analyses

Quantitative Real-Time Polymerase Chain Reaction, better known as qPCR, is the most sensitive and specific technique we have for the detection of nucleic acids. Even though it has been around for more than 30 years and is preferred in research applications, it has still to win broad acceptance in routine. Main hurdles are the lack of guidelines, standards, quality controls, and even proper methods to evaluate the diagnostic results. This is now rapidly changing.

In this course we go through the design and optimization of real-time PCR analytical tests, and validation of the entire testing workflow from the collection of the sample, nucleic acid extraction, transportation/storage, optional reverse transcription and qPCR. We also go through the analysis workflow from interpolate calibration, selection of normalization strategy, normalization and appropriate statistical testing.