How to Maintain an RNase-free Lab(Ambion)it is essential to develop a program of RNase control involving reagent preparation and testing, routine decontamination, and the use of certified RNase-free reagents and equipment such as microfuge tubes and pipette tips.http://www.ambion.com/techlib/tb/tb_180.htmlAdded: Thu May 08 2003, Hits: 1519, Reviews: 0Write reviewCached

Linear Polyacrylamide as Carrier in Ethanol Precipitation of Nucleic Acids
(Hideaki Shiraishi, Kyoto University)Linear polyacrylamide can be used as an efficient neutral carrier for precipitating nucleic acids with ethanol. Although glycogen is often used as carrier in ethanol precipitation, it has been shown that glycogen inhibits the activity of an ssDNA-binding protein on which linear polyacrylamide has no effect. Here is the method on how to prepare linear polyacrylamide used as carrier in ethanol precipitation of nucleic acids.
http://kuchem.kyoto-u.ac.jp/seika/shiraishi/protoc...Added: Fri Jan 30 2004, Hits: 1722, Reviews: 0Write reviewCached

RNase Control
(Ambion)Pinpoints some of the sources of RNase contamination and highlights ways to eliminate contamination problems at various stages while working with RNAhttp://www.ambion.com/techlib/basics/rnasecontrol/...Added: Thu May 08 2003, Hits: 802, Reviews: 0Write reviewCached

Solublization of RNA in Formamide
(NWFSC)Resuspending RNA in Formamide has several benefits over storage in water or ethanol. First, formamide will protect RNA from RNases. The RNA is also extremely stable... http://micro.nwfsc.noaa.gov/protocols/rnasoluble.h...Added: Tue May 14 2002, Hits: 761, Reviews: 0Write review