These experiments investigated the conditioning pattern and the nitrification efficiency of a fluidized sand biofilter (FSB) for seawater application. The FSB fed artificial nutrient was fully conditioned within 22 weeks. The maximum nitrification efficiency of the FSB was achieved at a superficial water velocity (SWV) of 1.0 cm/sec. After fixing the superficial water velocity at 1.0 cm/sec, the nitrification rates of the FSB were assessed at 3 total ammonia nitrogen (TAN) loading rates (250, 500, 1,000 g TAN//day) and 3 water temperatures (12, 16, ). The TAN concentration in the simulated culture tank ranged from 2.87 to 9.72 mg/L at TAN loading rate of 1,000 g TAN//day, while that ranged from 0.45 to 1.26 mg/L at TAN loading rate of 500 g TAN//day. The ranges of TAN concentration in the former were too high for aquatic organisms and those in the latter were acceptable. Therefore, the safe TAN loading rate for the FSB in seawater conditions was decided as 500 g TA//day. From these results, daily TAN removal rates (g TAN//day) of FSB under conditions of inlet TAN concentration (C, mg/L) and water temperature (T, ) were calculated by the following non-linear multi-regression equation: TAN removal rate: f(z)

The present study investigated the feces production rate and fecal nitrogen production ratio of abalone, Haliotis discus hannai fed seaweed, Undaria pinnatifida. The abalone, with shell lengths of 3 cm, 5 cm, and 7 cm, were tested at temperatures of , , and in a semi-recirculating aquaculture system. Under the given experimental conditions, the weight specific feces production rate (FPw) of the abalone increased with the decrease of shell length and the increase of temperature. The relationship of the FPw was. $FPw

The distributions of sanitary indicative bacteria and pathogenic bacteria in seawater and four species of farmed fishes, including oliver flounder (Paralichthys olivaceus), black rock fish (Sebastes schlegeli), red sea bream (Pagrus major) and sea bass (Lateolabrax japonicus), collected at fish farms located in the southern coastal area of Korea were investigated from May to October in 2004. The detection rates of fecal coliform and Entirococcus spp. of sanitary indicative bacteria in all samples were and , respectively. The occurrence of fecal coliform was highest of in Busan, Geoje and Wando area, followed Yeosu , Jeju , Tongyeong . The occurrence of Enterococcus spp. was highest In Wando area (), followed by Yeosu (), Tongyeong (), Busan (), Geoje and Jeju (). The detection rate of fecal coliform was higher than that of Enterococcus spp., except in the Tongyeong area. There was no difference in the detection rate of fecal coliform from May to October, but the detection rate of Enterococcus spp. increased with seasonal warming seawater temperature. Among the pathogenic bacteria, the detection rate of Vibrio alginolyticus () in all samples was highest, followed by V. parahaemolyticus (), Staphylococcus aureus (), Salmonella sp. (). However, V cholerae, V. vulnificus and Shigella sp. were not detected in all tested samples. The detection rates of V. parahaemolyticus and V. alginolyticus increased with seasonal warming seawater temperature from May to August.

The antimicrobial resistance patterns to the 10 antimicrobial agents of potential pathogenic vibrios isolated from seawater and a variety of farmed fishes, including oliver flounder (Payalichthys olivaceus), black rock fish (Sebastes schlegeli), red sea bream (Pagyus major) and sea bass (Lateolabyax japonicus), were investigated from May to October, 2004. A total of 314 strains of the genus vibrios were isolated from 126 collected samples, and the number of isolated strains of Vibrio payahaemolyticus and Vibrio alginolyticus were 194 and 120, respectively. Apparently of V. parahaemolyticus and of V. alginolyticus isolates demonstrated antimicrobial resistance against at least one antimicrobial agent. The resistance of V. parahaernolyticus isolates to ampicillin () was highest, followed by oxolinic acid (), amikacin () and tetracycline and amoxicillin/clavulanic acid (). V. alginolyticus isolates were resistant to ampicillin (), solfamethoxazoleit,imethopenem (), amikacln (), amoxicillin/clavulanic acid (), ciprofloxacin (), and tetracycline and doxycycline (). The rate of multiple antimicrobial resistance to at least four antimicrobials was higher in the V. alginolyticus isolates () than in the V. parahaemolyticus ().

An analysis method of tetracyclines far fish and shellfish products containing large amount of low molecular materials and pigments was established. The recovery of the established analysis method for four tetracycline samples was and higher than other methods reported. Especially, proposed sample treatment protocol was shown to be effective for the removal of low molecular materials and pigments that tend to interfere with accurate analysis. The detection limit of oxytetracycline (OTC) and tetracycline (TC) from the sample was 0.02 ppm, and the detection limit of chlortetracycline (CTC) and doxycycline (DC) from the sample was 0.1ppm, To examine the efficiency of the established method and identify tetracycline usage in fish farms, tetracycline group antibiotics in the flounder being cultured was monitored. The improved method can be used for fish and shellfish products effectively and all surveyed fish farms have used tetracycline all the year round. The proposed method was adopted as official method for fishery products by Korean Food and Drug Administration in 2003 and it is being used by regulatory authority as National Fishery Products Quality Inspection Service.

A high-performance liquid chromatography assay method for oxolinic acid in fish products was developed, evaluated and validated through the monitoring of oxolinic acid based on farming and distribution. The recovery rate of the developed method was as compared to conventional methods. The stock solution was stable for 3 weeks under refrigerated condition at The performance limit was evaluated as 0.01ppm of oxolinic acid in fish muscle. 478 fish samples such as olive flounder, genuine porgy, common sea bass and black rock fish collected from fish farms in the coastal area from September 2001 to October 2004 were analyzed to evaluate overall efficiency of the modified method and to monitor the actual condition of oxolinic acid usage in fish farm. According to the monitoring results, the modified method was suitable for analysis of oxolinic acid in fish muscle and oxolinic acid might be used in a small portion of fish farms. The suggested analysis method of oxolinic acid was registered in the Korean Official Methods of Food Analysis and is being utilized for fishery products by the Korea Food and Drug Adminstration and the National Fisheries Products Quality Inspection Service.

Seasonal variation of abundance and species composition of ichthyoplankton were studied in the coastal water off Tongyoung, Korea. Monthly samples were collected using a ring net which were towed horizontally at 4 different stations from March, 1998 to February, 1999. A total of 74 species representing 35 families and 8 orders were found. Of these species 40 species (17 families) were Perciformes. Of the fish larvae couected Engraulis japonicus, Repomucenus sp., Parablennius yatabei, Synechogobius hasta, and Tridentiger sp. were the dominant species representing in total number of individuals. The number of species was highest in August and lowest in December; number of individuals was highest in May and lowest in November. The diversity index (Shannon and Wiener Index) was highest in march, and lowest in December.

Codium fragile is commercially farmed in Korea by natural blooming zygote attachment. Experiments found optimum conditions for artificial seed production and nursery culture of C. fragile by asexual reproduction. Isolated utricles and medullary filaments were regenerated to erect thalli using both indoor and outdoor culture experiments. Under the indoor culture conditions, irradiance was an important factor to control the development of erect thalli. Formation of erect thallus from the isolated medullary filaments in the indoor culture was induced after 30 days under and . The detachment of isolated utricles and medullary filaments from the substrates of seed strings was reduced by exposure to the air during 2 hrs before the indoor culture of seed strings. The maximum growth and development of erect thalli in the nursery culture was induced at a water depth of 0.5 m. Depending on the substrates of the seed strings the growth of erect thalli was not significantly different (p>0.05).

This study investigated the composition and the distribution of the benthic polychaeta community in the semi-enclosed Yoja Bay on the southern coast of Korea and was designed to deduce temporal changes in the community with the comparison of the previous studies. In YoJa Bay, benthic polychaetous community structure was investigated on the base of the samples from 87 stations. The surface sediment was mainly composed of fine-grained sediment, mixed clayey silt and silty clay. The total benthic macrofaunal density was , and the density was higher in the middle bay, and in the mouth of bay than in the inner bay. scapharca subcrenata (Bivalvia) and amphipods (Crustacea) were important species in the inner and the middle of the bar, respectively. Benthic polychaetes were comprised of 72 species with a mean density of . Their distributions showed higher densities in the mouth of bay than in the rest of bay. The dominant species over 1.0 percentages were composed of the 20 species, and they occupied of the total density. The most dominant species was Sternaspis scutata (), fallowed fly Heteromastus filiformis (), Polynoidae indet. (), Sigambra tentacuzata () and others. The cluster analysis revealed that the study area was divided into three station groups. At the station group YI located in the inner bay, the most dominant species was Heteromastus filiformis . At the station group YII in the middle bay, the dominant species was Sternaspis scutata. And at the station group YIII in the mouth of bay, the dominant species was Paraiacydonia paradoxa. Whereas Sternaspis scutata and Mediomastus sp. were the dominant species in the past study, Heteromastus filiformis and Tharyx sp. appeared newly to be the dominant species, even if their low densities that in the past. These facts mean that Yoja Bay be maintained yet as little polluted area compared to other bays on the coast of Korea, but needed some caution of marine environmental management.

The study focused on the fluctuation of the fisheries conditions in fishing ground of yellow croaker, Pseudosciaena polyactis Bleeker. A long-term decreasing trend was observed in the accumulated catches of the species since 1926, with increasing-decreasing fluctuation repeating 6 times. Periodicity was observed in 10-year interval in good fishing years, but not in poor fishing years which had irregular fluctuations. From the geographical distribution of the past catch per unit effort, it was presumed that a group of the species seasonally migrated but most of the groups stayed throughout year in the western area off Cheju Island, which was different from the assumptions of the 1970`s. The fishing grounds were distributed along the migration route differently by season, throughout the Yellow Sea and the East China Sea, but shifted gradually southward and narrowed down to the area of off Cheju Island, where the center of fishing grounds was given birth to throughout the year with a relatively high density from autumn to spring. It was noted that a negative correlation was shown between the area of fishing grounds and density distribution of catch.