The present invention relates to novel chimeric fibroblast growth factors (FGF) wherein the alanine at amino acid 3 and serine 5 of native human recombinant basic fibroblast growth factor are replaced with glutamic acid. The N-terminus sequence of the present chimeric FGFs identify homology with that of human acidic fibroblast growth factor. The mitogenic properties of the native human recombinant basic FGF are exhibited by the present chimeric FGFs, and they are efficiently expressed in E. coli at significantly greater yields that previously reported. Novel variants of this new glu3,5 basic fibroblast growth factor, such as those in which cysteine 78 and cysteine 96 are replaced, e.g., with serine or other amino acids, to produce stabilized versions of the glu3,5 basic FGF and eliminate disulfide scrambled forms, are also described.

(a) a chimeric basic fibroblast growth factor having about 155 amino acids wherein the alanine at position 3 and the serine at position 5 are replaced with glutamic acid;

(b) a chimeric basic fibroblast growth factor having about 155 amino acids wherein the alanine at position 3 and the serine at position 5 are replaced with glutamic acid, and the cysteines at positions 78 and 96 are replaced with an amino acid selected from the group consisting of serine, lysine, aspartic acid, glutamic acid, asparagine, glutamine, histidine, isoleucine, leucine, valine, phenylalanine, tyrosine, methionine, threonine, proline, alanine, glycine, arginine and tryptophan; and

(c) the chimeric basic fibroblast growth factor of (a) or (b) having no N-terminal methionine;

wherein the amino acid at positions 78 and 96 are derivatized with a substituent selected from the group consisting of: CH2 COOH, CH(CO2 H) (CH2)x (CO2 H), CH2 CONR3 R4, R5, (CH2)n SO3, CHCH2 CONR3 CO(CH2)m NR3 R4, CH2 OCOCH2 R5 and SR6 ; wherein R3 and R4 are each H, (CH2)x CO2 H, CHCO2 H(CH2)x CO2 H or C1 -C6 alkyl optionally substituted with from 0 to 2 hydroxyl groups or polyethylene glycol; R5 is C1 -C6 alkyl or C1 -C4 alkoxymethyl and R6 is C1 -C6 alkyl, polyethylene glycol or phenyl optionally substituted with one or two carboxylic acid or sulfuric acid groups; n is an integer of from 0 to 4; m is an integer of from 2 to 4; and x is an integer of from 1 to 3.

This is a continuation-in-part of copending application(s) Ser. No. 07/615,202 filed on Nov. 23, 1990, now abandoned.

BACKGROUND OF THE INVENTION

The present invention relates to novel chimeric basic fibroblast growth factors and to the enhanced production of such factors (bFGF).

Polypeptide growth factors are hormone-like modulators of cell proliferation and differentiation. Growth factors are responsible for the regulation of a variety of physiological processes, including development, regeneration and wound repair.

In the course of study of these factors, a number have been identified on the basis of the ability of extracts from various tissues, such as brain, pituitary and hypothalamus, to stimulate the mitosis of cultured cells. Numerous shorthand names have been applied to active factors in these extracts, including epidermal growth factor, platelet-derived growth factor, nerve growth factor, hematopoietic growth factor and fibroblast growth factor.

Fibroblast growth factor (FGF) was first described by Gospodarowicz in 1974 (Nature 249: 123-127) as derived from bovine brain or pituitary tissue which was mitogenic for fibroblasts and endothelial cells. It was later noted that the primary mitogen from brain was different from that isolated from pituitary. These two factors were named acidic and basic FGF, respectively, because they had similar if not identical biological activities but differed in their isolectric points. Acidic and basic fibroblast growth factors (recently reviewed by Burgess, W. H., and Maciag, T. Ann. Rev. Biochem. 58: 575-606 (1989)) appear to be normal members of a family of heparin-binding growth factors that influence the general proliferation capacity of a majority of mesoderm- and neuroectoderm-derived cells (Gospodarowicz, D., et al., Nat. Cancer Inst. Mon. 48:109-130 (1978)), including endothelial cells, smooth muscle cells, adrenal cortex cells, prostatic and retina epithelial cells, oligodendrocytes, astrocytes, chrondocytes, myoblasts and osteoblasts (Burgess and Maciag, cited above at page 584). Although human melanocytes respond to the mitogenic influences of basic fibroblast growth factor but not acidic FGF most avian and mammalian cell types respond to both polypeptides (ibid.).

In addition to eliciting a mitogenic response that stimulates cell growth, fibroblast growth factors can stimulate a large number of cell types to respond in a non-mitogenic manner. These activities include promotion of cell migration into wound areas (chemotaxis), initiation of new blood vessel formation (angiogenesis), modulation of nerve regeneration (neurotropism), and stimulation or suppression of specific cellular protein expression, extracellular matrix production and cell survival important in the healing process (Burgess and Maciag, cited above, pages 584 to 588).

A number of basic fibroblast growth factor analogues have been suggested. Muteins of bFGF having amino or carboxyl terminal amino acids deleted, amino acids added, cysteine substituted with a neutral amino acid such as serine, or aspartic acid, arginine, glycine, serine, or valine substituted with other acids have been suggested to have enhanced stability (Eur. Pat. Ap. Pub. No. 281,822 to Seno, et al., page 4, lines 1 to 3, and page 6, line 29 to page 7, line 19); the muteins comprise two or three additions, deletions or substitutions, with substitution of serine for cysteine the most preferred substitution (page 7, lines 18 to 23). Arakawa and Fox (Eur Pat. Ap. Pub. No. 320, 148) suggested replacing at least one, and more preferably two, of the cysteines found in natural bFGF with a different amino acid residue to yield a more stable analogue (page 4, lines 44 to 47); serine was illustrated in the Examples (page 13, lines 22 to 23), but alanine, aspartic acid and asparagine were also suggested (page 5, line 26 and page 13, line 25). Similarly, recombinant aFGFs having extraneous bond-forming cysteine replaced with serine, and oxidation-prone cysteine, methionine and tryptophan replaced with alanine, valine, leucine or isoleucine, to yield factors having enhanced or improved biological activity have also been suggested (Eur. Pat. Ap. Pub. No. 319,052 to Thomas Jnr and Linemeyer, page 17, lines 8 to 20).

However, new stable and active forms of fibroblast growth factors are increasingly sought to use in the therapies indicated hereinabove.

SUMMARY OF INVENTION

The present invention relates to novel, full length (coding for 155 amino acid) human basic fibroblast growth factor genes and proteins which have the alanine residue at position 3 and the serine residue at position 5 of the native bFGF replaced with glutamic acid. Glu3,5 hbFGF of this invention hares sequence identity with human acidic FGF at the N-terminal 8 amino acids and can thus be considered a chimeric FGF. More specifically, these factors are of human FGF but other mammalian species of FGFs are available through the present invention.

The glu3,5 chimeric fibroblast growth factor has the mitogenic properties of tissue-derived bFGF, but expression in E. coli is significantly greater than the native sequence. Thus, this invention provides novel, biologically active FGF and a method of preparing it in high yield.

The same finding exists with respect to novel variants of glu3,5 hbFGF For example, a stabilized version of the growth factor is prepared by replacing cysteine 78 and cysteine 96 with amino acids that eliminate thiol-disulfide interchange (disulfide scrambling), such as serine. Thus, this invention not only modifies hbFGF (1-155) to significantly increase the yield of the factor expressed in E. coli, but also facilitates purification and enhances stability.

Therefore, this invention provides novel, biologically active fibroblast growth factors and methods of preparing these on a preparative scale. In a preferred embodiment, DNA encoding novel FGF of this invention is inserted into plasmids or vectors, which may be conveniently and efficiently conserved, stored or transported, if desired. The plasmids or vectors are then used to transform or transfect microorganism, e.g., E coli. which likewise may be used to conserve, store, or transport, if desired, the genomic material encoding the novel FGF of this invention. Culture of these microorganisms under conditions that express the factors yield the polypeptides in abundance.

Since, as described above, growth factors released into traumatized areas accelerate the normal healing process, the novel fibroblast growth factors of this invention have therapeutic applications for healing burns, surgical incisions, and other wounds; for treating skin ulcers, including bedsores and the like; for cardiovascular conditions and restarting blood flow after heart attacks by revascularizing the damaged tissue; for enhancing bone repair and treating musculoskeletal injuries; and in neurodegenerative and other disease states.

DETAILED DESCRIPTION OF THE INVENTION

FIG. 1. Construction of Glu3,5,Ser78,96 hbFGF cDNA. Using the expression plasmid pT7 glu3,5 hbFGF prepared in Example 2 as a template, cys78 to ser78 and cys96 to ser96 mutations are directed using the following polymerase chain reaction mixtures: (1) T7 sense plus ser78 antisense primers; and (2) T7 antisense plus ser96 sense primers. A polymerase chain reaction is then performed from (1) and (2) using T7 sense and T7 antisense primers as described in Example 3.

FIG. 2. Heparin HPLC of Natural hbFGF. Bound hbFGF (containing 154 amino acids) is eluted from a heparin sepharose column using a linear 0.6 to 3.0M NaCl gradient at 0.7 ml/min and monitored at 280 nm as described in Example 5.

FIG. 3. Heparin HPLC of Reduced Recombinant hbFGF. An elution profile from heparin HPLC of a portion of pooled material from heparin sepharose chromatography that has been reduced with dithiotheitol as described in Example 6.

FIG. 4. Reverse Phase HPLC of Glu3,5,Ser78,96 hbFGF. A sample from heparin HPLC (8 μg) is loaded onto a 0.45×25 cm Vydac C4 column and eluted at 0.7 ml/min using a 0.1% trifluoroacetic acid/acetonitrile solvent system (0 to 28% acetonitrile in 15 min, 28 to 60% in 99 min, and 60 to 80% in 10 min) as described in Example 6.

FIG. 5. Bioassay Comparison of native bFGF with Recombinant bFGF's. The mitogenic activity of bFGF isolated from bovine brain is compared with human recombinant bFGF's on the proliferation of aortic arch bovine vascular endothelial cells as described in Example 7. Cells are grown in the presence of different amounts of bovine brain bFGF (10-155) (- -); natural sequence recombinant hbFGF (- -); and glu3,5 hbFGF (-o-) (determined by amino acids analysis) as indicated. After 4 days, acid phosphatase activity , equivalent to cell number over the cell density range examined, is determined at 405 nm.

FIG. 6. Bioassay Comparison of native bFGF with Chimeric bFGF's. The mitogenic activity of bovine brain bFGF (10-155) (-o-) and glu3,5,ser78,96 hbFGF (- -) is compared using cells maintained in the presence of different amounts (determined by amino acids analysis) of growth factors as indicated for 5 days and cell number determined as described in Example 7.

DETAILED DESCRIPTION OF THE INVENTION

The present invention relates to the enhanced production and stabilization of new, basic fibroblast growth factors having about 155 amino acids. Although the human recombinant example provided herein, the FGF of the present invention is applicable to other mammalian species. A novel recombinant hbFGF of this invention is prepared by replacing alanine 3 and serine 5 with glutamic acid in full length human basic fibroblast growth factor. Glu3,5 hbFGF shares sequence identity with human aFGF at the N-terminal 8 amino acids and is thus considered a chimeric analogue of haFGF and hbFGF. This mutation significantly increases protein yield expressed in Escherichia coli as compared to native sequence bFGF.

Both recombinant native bFGF and glu3,5 hbFGF exhibit microheterogeneity on heparin- and reverse phase- high performance liquid chromatography. While not wishing to be bound to any theory, this microheterogeneity appears to be due to thiol-disulfide interchange (disulfide scrambling) because it can be eliminated by treatment of the growth factors with a reducing agent prior to chromatography. Generation of a stabilized version of the growth factor and elimination of disulfide scrambled forms is accomplished by replacement of cysteine 78 and cysteine 96 with serine by site-directed mutagenesis.

A protein is defined herein as basic FGF if it shows FGF activity in in vitro and in vivo assays (summarized by Burgess and Maciag, cited above, pages 584 to 586); binds to heparin; and is eluted from heparin sepharose at 1.5-1.7M NaCl; and reacts immunologically with antibodies prepared using human or bovine basic FGF or synthetic or native peptides thereof, or to synthetic analogues of bFGF sequences conjugated to bovine serum albumin. A protein is defined herein as acidic FGF if it shows FGF activity in in vitro and in vivo assays; binds to heparin; and is eluted at 1.0-1.2M NaCl from heparin sepharose; and is immunologically reactive with antibodies prepared against human or bovine aFGF or against synthetic or native peptides thereof. A chimeric fibroblast growth factor shares the sequence of both types. Any type of mammalian fibroblast growth factor is encompassed by this invention, particularly human fibroblast growth factor.

The chimeric fibroblast growth factors of this invention include glu3,5 hbFGF and glu3,5,ser78,96 hbFGF having about 155 amino acids, and truncated forms having about 154 amino acids (e.g., those having no N-terminal methionine; see Example 6 (b)). This invention also encompasses glu3,5 hbFGF analogues having the cysteine residues at positions 78 and 96 replaced with other amino acids, such as, for example alanine, glycine, arginine, tryptophan, lysine, aspartic acid, glutamic acid, asparagine, glutamine, histidine, isoleucine, leucine, valine, phenylalanine, tyrosine, methionine, serine, threonine or proline. Moreover, the FGF derivatives of this invention are not species specific, and include, for example, bovine FGF counterparts and others that share similar sequence homology with hbFGF.

The novel fibroblast growth factors of this invention may be prepared by assembling polypeptides from constituent amino acids, or from amino acids or peptides and polypeptides, using chemical or biochemical means known to those skilled in the art, such as, for example, by adding amino acids sequentially to shorter fibroblast forms at the N-terminus. Alternatively, the novel fibroblast growth factors of this invention may be prepared by recombinant protein synthesis involving preparation of DNA encoding chimeric FGF, insertion of the DNA into a vector, expression of the vector in host cells, and isolation of the recombinant FGF thereby produced.

DNA encoding the FGF of this invention may be prepared by altering a gene of human or bovine basic fibroblast growth factor by nucleotide deletions, nucleotide additions, or point mutations produced using standard means. An illustration is set out in Example 1. Because of the degeneracy of the genetic code, a variety of codon change combinations can be selected to form DNA that encodes the FGF of this invention, so that any nucleotide deletion(s), addition(s), or point mutation(s) that result in a DNA encoding chimeric FGF are encompassed by this invention. Since certain codons are more efficient for polypeptide expression in certain types of organisms, the selection of fibroblast gene alterations to yield DNA material that codes for the FGF of this invention are preferably those that yield the most efficient expression in the type of organism which is to serve as the host of the recombinant vector. Altered codon selection may also depend upon vector construction considerations.

Fibroblast growth factor DNA starting material which can be altered to form chimeric DNA may be natural, recombinant or synthetic. Thus, DNA starting material may be isolated from tissue or tissue culture, constructed from oligonucleotides using conventional methods, obtained commercially, or prepared by isolating RNA coding for bFGF from fibroblasts, using this RNA to synthesize single-stranded cDNA which can be used as a template to synthesize the corresponding double stranded DNA.

Illustrating the present invention are cloned complementary DNA sequences defining chimeric fibroblast polypeptide sequences such as that constructed in Examples 2 and 3. Also encompassed are DNA sequences homologous or closely related to complementary DNA described herein, namely DNA sequences which hybridize, particularly under stringent conditions, to chimeric fibroblast cDNA, and RNA corresponding thereto. In addition to the chimeric FGF-encoding sequences, DNA encompassed by this invention may contain additional sequences, depending upon vector construction sequences, that facilitate expression of the gene.

DNA encoding the chimeric growth factors of this invention, or RNA corresponding thereto, are then inserted into a vector, e.g., a pBR, pUC, pUB or pET series plasmid, and the recombinant vector used to transform a microbial host organisms. Host organisms may be bacterial (e.g., E. coli or E. subtilis), yeast (e.g., S. cervisiae or mammalian (e.g., mouse fibroblast). This invention thus also provides novel, biologically functional viral and circular plasmid RNA and DNA vectors incorporating RNA and DNA sequences describing the chimeric growth factors generated by standard means. Culture of host organisms stably transformed or transfected with such vectors under conditions facilitative of large scale expression of the exogenous, vector-borne DNA or RNA sequences and isolation of the desired polypeptides from the growth medium, cellular lysates, or cellular membrane fractions yields the desired products. An example of expression of hbFGF mutants in E. coli is given in Example 4.

The present invention provides for the total and/or partial manufacture of DNA sequences coding for glu3,5 hbFGF, glu3,5, ser78,96 hbFGF, and other glu3,5 hbFGF having the cysteines at positions 78 and 96 replaced with other amino acids that eliminate disulfide scrambling, and including such advantageous characteristics as incorporation of codons preferred for expression by selected non-mammalian hosts, provision of sites of cleavage by restriction by endonuclease enzymes, and provision of additional initial, terminal or intermediate DNA sequences which facilitate construction of readily expressed vectors. Correspondingly, the present invention provides for manufacture (and development by site specific mutagenesis of cDNA and genomic DNA) of DNA sequences coding for microbial expression of chimeric fibroblast growth factor which differ from the forms specifically described herein in terms of identity or location of one or more amino acids residues (i.e., deletion analogues containing less than all of the residues specified for hbFGF, and/or substitution analogues wherein one or more residues are replaced by and/or addition analogues wherein one or more residues are added to a terminal or medial portion of the polypeptide), and which share the biological properties of glu3,5 hbFGF and glu3,5, ser78,96 hbFGF.

DNA (and RNA) sequences of this invention code for all sequences useful in securing expression in procaryotic or eucaryotic host cells of polypeptide products having at least a part of the primary structural conformation, and one or more of the biological properties of chimeric fibroblast growth factor which are comprehended by: (a) the DNA sequenced encoding glu3,5 hbFGF and glu3,5,ser78,96 hbFGF as described herein, or complementary strands; (b) DNA sequences which hybridize (under hybridization conditions as described herein or more stringent conditions) to DNA sequences defined in (a) or fragments thereof; and (c) DNA sequences which, but for the degeneracy of the genetic code, would hybridize to the DNA sequenced defined in (a) and (b) above. Specifically comprehended are genomic DNA sequenced encoding allelic variant forms of chimeric fibroblast growth factors included therein, and sequences encoding chimeric fibroblast growth factor RNA, fragments thereof, and analogues wherein RNA or DNA sequenced may incorporate codon facilitating transcription or RNA replication of messenger RNA in non-vertebrate hosts.

Isolation and purification of microbially expressed polypeptides provided by the invention may be by conventional means including, for example, preparative chromatographic separations such as that illustrated in FIGS. 2 and 3, and immunological separations, including monoclonal and/or polyclonal antibody preparations, i.e. example purification is given in Example 5.

As summarized above and described in detail in the Examples below, and example chimeric fibroblast growth factor of this invention is full length (155 amino acids) human recombinant basic fibroblast growth factor having alanine 3 and serine 5 replaced with glutamic acid expressed, using the T7 RNA polymerase expression system, in E. coli. (The numbering for bFGF adopted here is for the 155 amino acid form as described in Abraham et al, 1986 and refers to the methionine codon as position 1) Both recombinant native bFGF and glu3,5 hbFGF exhibit extensive microheterogeneity on heparin- and RP-PHLC (FIG. 2) which is eliminated by treatment of the growth factor with a reducing agent such as dithiothreitol prior to chromatography (FIG. 3). Generation of a stabilized version of the growth factor and elimination of disulfide scrambled forms is accomplished by replacement of cysteine 78 and cysteine 96 with serine by site-directed mutagenesis (Example 3).

The yield of both glu3,5 hbFGF and glu3,5,ser78,96 hbFGF in this expression system, like aFGF cDNA, is 10-fold higher than parental bFGF cDNA (Example 5). Gu3,5 hbFGF and glu3,5,ser78,96 hbFGF share sequence identity with haFGF at the N-terminal 8 amino acids. Thus, these derivatives are chimeric.

The polypeptides of this invention retain biological activity as fibroblast growth factors. For example when the mitogenic properties of recombinant hFGF and mutant proteins are compared to bFGF (10-155) originally isolated from bovine brain (Example 7), human recombinant bFGF and glu3,5 hbFGF show a dose-dependent stimulation of endothelial cell growth that was essentially identical to that for bovine brain bFGF (FIG. 5). Replacement of cysteine 78 and 96 with serine to give glu3,5,ser78,96 hbFGF had no effect on the mitogenic potency and gave a dose-response curve that was indistinguishable from that determined for tissue-derived bovine bFGF (FIG. 6). Sequence I.D. Numbers 1 and 2 disclose the sequences of glu3,5 hbFGF and glu3,5, Ser78,96 hbFGF, respectively.

The modifications to hbFGF described herein significantly increase the yield of growth factor expressed, facilitate its purification, eliminates microheterogeniety due to disulfide scrambling, and enhances stability while retaining full biological activity.

EXAMPLES

The following examples are presented to further describe and explain the present invention and the characterization techniques employed, and should not be taken as limiting in any regard. Unless otherwise indicated, all parts and percentages are by weight, and are based on the weight at the particular stage of the processing being described.

EXAMPLE 1Construction of an Expression Plasmid

A synthetic gene encoding the 155 amino acid form of human bFGF (Abraham, J. A., et al, EMBO J. 5: 2523-2528 (1986)) cloned into pUC 18 was purchased from British Bio-technology, Oxford, UK. Destruction of the internal Nco1 restriction site at positions -2 to 3, which includes the N-terminal methionine codon of the bFGF cDNA, and introduction of a unique Nde1 site is as follows. The nucleotide sequence (-12 to 36) to be changed (a, below) is excised from pUC 18 with HindIII and BspMII and a synthetic fragment (b, below) containing an internal Nde1 site cloned into pUC 18. This cloning results in a construct that contains a 4 nucleotide deletion in the upstream non-coding region compared to the original construct (see below). This deletion has no effect on the relative protein yields of bFGF using the expression system described below.

5' AGCTTACCTGCCATGGCAGCCGGGAGCATCACCACGCTGCCCGCCCTT 3' (a)

5' AGCTTCATATGGCAGCCGGGAGCATCACCACGCTGCCCGCCCTT 3' (b)

Only the sense strands are shown for the original (a) and modified (b) fragments, respectively. The codon underlined indicates the position of the methionine start codon.

The cDNA encoding bFGF is then excised from pUC 18 with Nde1 and BamH1 and cloned into the Nde1 and BamH1 sites of the expression vector pT7 Kan 5, derivative of pET-3a (plasmid for Expression by bacteriophage T7, as defined in Rosenberg, A., et al, Gene 56: 125-135 (1987) at page 128) containing the T7 promoter for RNA polymerase.

EXAMPLE 2Construction of Glu3,5 hbFGF

The protocol for the construction of glu3,5 hbFGF is identical to that described above for the introduction of the Nde1 restriction site except that the region encoding the first 5-terminal amino acids of basic FGF (c) are changed to encode those of acidic FGF (d):

5' AGCTTCATATGGCAGCCGGGAGCATCACCACGCTGCCCGCCCTT 3' (c)

5' AGCTTCATATGGCTGAAGGGGAAATCACCACGCTGCCCGCCCTT 3' (d)

Only the sense strands are shown for the original (c) and modified (d) fragments, respectively. The codons underlined indicate those changed to encode glutamic acid at positions 3 and 5.

EXAMPLE 3Construction of Glu3,5 Ser78,96 hbFGF

The expression plasmid pT7 glu3,5 hbFGF is used as a template for oligonucleotide site-directed mutagenesis. Two mutagenic oligonucleotide primers are designed to change cysteine codons at positions 78 and 96 to serine codons. The primer for serine at position 96 is to the sense strand (60-mer; 238-297) whereas that for serine at position 78 is to the anti-sense strand (30-mer; 251-222). In addition to these mutagenic primers, primers to the T7 promotor (nucleotide -12 to +13) and terminator regions (nucleotide -75 to -54) are designed (19).

Excess primers are separated from the amplified DNA fragments by 3 successive rounds of concentration and dialysis using 30,000 molecular weight Millipore microconcentrators. Portions of the retentates are combined and amplified using the PCR as described above except that the primers used correspond to the T7 promoter (sense) and T7 terminator (antisense) regions. See FIG. 1. The 599 basepair PCR product is then treated with NdeI and BamHI and purified by agarose gel electrophoresis. The purified fragment is then cloned into the T7 expression vector, pET-3a(M13), a derivative of pET-3a.

Cell pellets from 1 liter cultures are resuspended in 30 ml 50 mM Tris, 0.1 mM EDTA buffer, pH 7.6, and lysed by 3 rapid freeze/thaw cycles. The lysate is then treated with DNase I (20 μg/ml) in the presence of 5 mM MgCl2 for 20 min at 4° C. and centrifuged at 10,000 ×g for 20 min to remove cell debris. bFGF activity is found to be equally distributed in the pellet and supernatant fractions.

FGF is purified from the supernatant solution by heparin-sepharose column chromatography as described by Gospodarowicz, D., et al., Proc. Nat. Acad. Sci. USA 81:6963-6967 (1984), and eluting with a linear salt gradient from 0.6 to 3.0M NaCl. The fractions containing growth factor are pooled and diluted with 10 mM, pH 7.6 Tris buffer to give a final NaCl concentration of about 0.6M.

This is loaded onto a 0.75×7.5 cm TSK Heparin-5PW column (TosoHaas, Philadelphia, PA) equilibrated with 10 mM, pH 7.6 Tris, 0.6M NaCl. Elution of bound material is monitored at 280 nm and is accomplished using a linear salt gradient (0.6 to 3.0M NaCl in 60 min) at a flow rate of 0.7 ml/min.

Using the T7 expression system described in Example 4, the yield of native sequence hbFGF (2-155) is about 0.8 mg/1 bacterial culture. With native sequence haFGF, a 6 to 8 mg/liter yield is obtained. Glu3,5 hbFGF expressed in the same system gives 8 to 10 mg/liter of purified factor.

Large scale fermentation (10L) of E. coli containing the plasmid pT7 glu3,5,ser78,96 gives about 1 mg of purified growth factor per g of cell paste. Protein yield of the chimeric ser78,96 variant and distribution of the protein in the supernatant and pellet fractions of the bacterial extract are similar to that observed for glu3,5 hbFGF.

The elution profile from heparin sepharose chromatography of a crude cell homogenate containing native sequence hbFGF (2-155) shows two major protein peaks both of which possess mitogenic activity and contain a major protein species of Mr 17,000 by sodium dodeoyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). C4 reverse phase-high performance liquid chromatorgaphy (RP-HPLC) of material from each of the two peaks obtained from the heparin sepharose step followed by N-terminal sequence analysis of the resolved components identify at least 3 distinct forms of bFGF.

As a first approach to analyze this apparent microheterogenetity, the contribution of thiol-disulfide interchange (disulfide scrambling) in the generation of chromatographically distinct species is assessed by treatment with a reducing agent. Incubation of a portion of heparin sepharose purified bFGF with dithiothreitol (2 mM) for 10 min at 37° C. followed by RP-HPLC analysis shows that the peaks previously identified as bFGF species chromatograph essentially as a single peak.

High resolution TSK Heparin HPLC of 2 protein peaks containing FGF from the heparin sepharose step reveals 4 major protein components that elute over a range of 1.6 to 2.3M NaCl (FIG. 2). Analysis by SDS-PAGE of these peaks shows P-I, P-III and P-IV to contain a single protein band that migrates with an Mr of 17,000 consistent with that hbFGF(2-155), whereas PII exhibits an Mr of about 22,000 and is identified by N-terminal sequence analysis as a contaminant. Treatment of a portion of the pooled material from heparin sepharose chromatography with dithiothreitol (5 mM) for 10 min at room temperature followed by heparin HPLC shows an increase in the amount of P-I, a reduction in that of P-III and the disappearance of P-IV; the position and intensity of P-II containing the Mr 22,000 impurity is unaffected by this treatment (FIG. 3).

The chromatographic behavior of the glu3,5 hbFGF in the presence and absence of dithiothreitol on heparin and RP-HPLC is similar to that observed for native sequence hbFGF. The cysteine to serine mutation greatly facilitates the purification of this analogue since it behaves as a single species on heparin- and C4 RP-HPLC (FIG. 4) and thus eliminates the need for dithiothreitol treatment during purification.

isolated from heparin HPLC gives a single sequence consistent with glu3,5 hbFGF (2-155) indicating complete removal of the N-terminal methionine.

(c) Molecular Weights

Molecular weight determinations are performed on a 10 to 15% gradient and 20% homogeneous polyacrylamide gels in the presence of sodium dodecyl sulfate (SDS-PAGE) using a silver stain detection system (Phastgel System, Pharmacia/LKB).

hbFGF (2-155) migrates with an Mr of 17,000 compared to an Mr value of about 19,000 for glu3,5 hbFGF. Molecular weights calculated from amino acid sequence data for hbFGF and the chimeric version are 17,124 and 7,224, respectively. To resolve the apparent molecular weight discrepancy, a sample of glu3,5 hbFGF is analyzed by liquid-secondary ion mass spectrometry and gives a molecular ion of mass 17,365. This value is, within experimental error, consistent with that predicted from sequence data. While not wishing to be bound to any theory, the anomalous migration of glu3,5 on polyacrylamide gels under denaturing conditions is most likely due to interference of protein-SDS interactions from the glutamyl side chains at positions 3 and 5.

On SDS-PAGE glu3,5,ser78,96 hbFGF also migrates as an Mr 19,000 protein and not as a predicted Mr 17,000 species. This observation is consistent with the aberrant migration noted for glu3,5 hbFGF.

After 4 to 5 days in culture, each well is washed and 100 μl pH 5.5 buffer containing 0.1M sodium acetate, 0.1% Triton X-100 and 10 mM p-nitrophenyl phosphate (Sigma 104 phosphatase substrate) are added to each well. The plates are incubated at 37° C. for 2 hours, the reaction stopped by adding 10 μl of 1 N sodium hydroxide, and color development determined at 405 nm against a buffer blank incubated without cells using a UV max kinetic microplate reader (Molecular Devices, CA). Determinations are made in triplicate. Both methods give indistinguishable dose-response curves.

When the mitogenic properties of recombinant hbFGF and mutant proteins are compared to those of bFGF (10-155) originally isolated from bovine brain, human recombinant bFGF and glu3,5 hbFGF show a dose-dependent stimulation of endothelial cell growth that is essentially identical to that for bovine brain bFGF (FIG. 5) and exhibit doses for half-maximal stimulation (median effective dose, ED50) of 0.3 to 1.0 ng/ml and a maximal stimulation between 3 and 10 ng/ml. Replacement of cysteine 78 and 96 with serine to give glu3,5,ser78,96 hbFGF has no effect on the mitogenic potency and gives a dose-response curve that is indistinguishable from that determined for tissue-derived bovine bFGF (FIG. 6).

The above description is for the purpose of teaching the person of ordinary skill in the art how to practice the present invention and it is not intended to detail all those obvious modifications and variations of it which will become apparent to the skilled worker upon reading the description. It is intended, however, that all such obvious modifications and variations be included within the scope of the present invention as defined in the appended claims.

The DNA sequences, plasmids and/or microorganisms deposited in connection with the present patent application, except where specified to the contrary, are deposited in American Cyanamid Company's culture collection maintained in Pearl River, N.Y. and are available to the public when legally appropriate to do so. Further, the following are deposited additionally with the American Type Culture Collection (ATCC), 12301 Parklawn Drive, Rockville, Md. 20852, U.S.A. on the date indicated with the ATCC accession numbers indicated:

Glu3,5 hbFGF (5mg/ml) in 1.0M Tris buffer pH8.6 containing 2mM EDTA is reduced by addition of dithiothreitol (5MM) and incurbated for 1h at room temperature under an argon atomosphere. MeO-PEG-O2 -CCH2 I (MW=2000 or =5000) MeO-PEG-NHCOCH2 I (MW=5000) is added to give a final concentration of 25-50 mM and the reaction mixture isthen dialysed against phoshate buffered saline (PBS) at 4° C. for 12h.

EXAMPLE 9Derivatized FGF

Glu3,5 hbFGF (5 mg/ml in 10 mM Tris buffer pH7.4 containing 1.5M NaC1 is reduced by addition of dithiothreitol (5 mM) and incubated for 0.5-1h at room temperature under an argon atomosphere. Iodoacetic acid () 0.4M in 1M Tris buffer ph8.5 ) is then added to give a final concentration of 50 mM and the reaction mixture incubated in the dark for 2h at room temperature. The solution is then dialysed against 10 mM Tris buffer (pH 7.0) containing 0.5M NaC1 for 12h.

Both the polyethylene glycol derivative of bFGF and the carboxymethylated bFGF are assayed as described hereinabove.

EXAMPLE 10Carboxymethylated FGF

Carboxymethylated bFGF: Treatment of Glu3,5 hbFGF (2-155) with iodoacetic acid under non-denaturing conditions results in the carboxymethylation of 2 of the 4 cysteine residues of bFGF. The positions of modified cysteines are identified as cysteine 78 and 96 by peptide mapping of a endoproteinase Glu-C digest of 14 C-labelled carboxymethylated bFGF. Modification of cysteine 78 and 96 has no effect on the affinity of bFGF for heparin. The mitogenic activity and receptor binding properties of Glu3,5 CMCys78,96 hbFGF are indistinguishable from that of Glu3,5 hbFGF (FIG. 1) whereas fully carboxymethylated bFGF does not seem active. In contrast to unmodified bFGF, Glu3,5 hbFGF has a half-life at p 4 of about 5 min, whereas that for Glu3,5 CMCys78,96 hbFGF is >60 min (see FIG. 2).

Polyethylene glycol amide of bFGF: PEG-5000 derivative of glu3,5 hbFGF in contrast to the PEG esters of bFGF are stable. They are fully active in BalbC 3T3 fibroblast mitogenesis assays and compete as effectively as unmodified glu3,5 hbFGF in FGF receptor binding assays.

Method for treating a human patient for coronary artery disease, comprising administering into one or more coronary vessels in a human patient in need of treatment for coronary artery disease an effective amount of a recombinant fibroblast

Protein selected from group consisting of residues 66 through 208 of specified amino acid sequence and residues 66 through 208 of same sequence and n-terminal methionine; stimulates production of epithelial cells

Method for treating a human patient for coronary artery disease, comprising administering into one or more coronary vessels in a human patient in need of treatment for coronary artery disease an effective amount of a recombinant fibroblast