Monoclonal antibodies were produced from a hybridoma resulting from the fusion of a mouse
myeloma with B cells obtained from a mouse immunized with purified, E. coli-derived,
recombinant human FGF-basic.

The lyophilized antibody is stable at room temperature for at least one month and for greater
than a year when kept at -800C.

Reconstitution:

When reconstituted in sterile water to a concentration of 1 mg/ml the antibody is stable for at
least six weeks at 2-40C.

Applications

ELISA:

In a sandwich ELISA (assuming 100μl/well), a concentration of 2 μg/ml of this antibody will
detect at least 0.2 ng/well of recombinant human FGF-basic when used with PeproTech (Rocky
Hill, NJ) biotinylated antigen affinity purified anti-human FGF-basic as the detection antibody
at a concentration of 1 μg/ml.