* Many materials used in wet biology are flammable or otherwise harmful and will be kept in a locked cabinet labeled with the appropriate warning labels (ie: flammable, skull and crossbones, etc.). Flammables, acids, and bases should be stored in a segregated manner.

* Many materials used in wet biology are flammable or otherwise harmful and will be kept in a locked cabinet labeled with the appropriate warning labels (ie: flammable, skull and crossbones, etc.). Flammables, acids, and bases should be stored in a segregated manner.

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* Safety Material Data Sheets will be kept near the cabinet for all materials stored there.

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* Material Safety Data Sheets will be kept near the cabinet for all materials stored there.

* Noisebridge is a humane space -- no animal experimentation!

* Noisebridge is a humane space -- no animal experimentation!

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* '''Extra caution must be used in dealing with chemicals and cell cultures for not just healthy adults, but also children, elderly, immunosuppressed people, people with high allergen reactions, pets, and anything the pets may ingest including roaches/mice/pests that may reside at Noisebridge. All of these things come into Noisebridge and we can by NO MEANS be thought of as lab environment (until we come up with dedicated space or that).'''

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* '''Extra caution must be used in dealing with chemicals and cell cultures for not just healthy adults, but also children, elderly, immunosuppressed people, people with high allergen reactions, pets, and anything the pets may ingest including roaches/mice/pests that may reside at Noisebridge. All of these things come into Noisebridge and we can by NO MEANS be thought of as lab environment (until we come up with dedicated space for that).'''

* ''Know your materials, know your materials, know your materials''

* ''Know your materials, know your materials, know your materials''

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All waste that has come in contact with bacteria, yeast, or transgenic cells is considered biohazard waste. Biohazard waste must be sterilized before being disposed of. The following two methods of sterilization may be used:

All waste that has come in contact with bacteria, yeast, or transgenic cells is considered biohazard waste. Biohazard waste must be sterilized before being disposed of. The following two methods of sterilization may be used:

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==== Pressure Cooker ====

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==== Pressure Cooker Sterilization ====

The pressure cooker may be used to sterilize liquid and solid waste, as well as to sterilize media, glassware, tools etc. The procedures for use are as follows:

The pressure cooker may be used to sterilize liquid and solid waste, as well as to sterilize media, glassware, tools etc. The procedures for use are as follows:

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Following sterilization, solid waste bags may be disposed of in regular trash, and liquid waste may be poured down the drain.

Following sterilization, solid waste bags may be disposed of in regular trash, and liquid waste may be poured down the drain.

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==== Liquid Bleach ====

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==== Liquid Bleach Sterilization ====

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Liquid solutions may be sterilized adding 10% bleach to the solution and letting sit for 20 minutes. Following sterilization, liquid waste may be poured down the drain.

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Liquid solutions may be sterilized by adding 10% bleach to the solution and letting sit for 20 minutes. Following sterilization, liquid waste may be poured down the drain.

=== Chemical Waste ===

=== Chemical Waste ===

Noisebridge currently does not have the permits or processes necessary to handle hazardous waste generation and disposal. Therefore no chemicals or reagents requiring hazardous waste disposal should be brought into Noisebridge until such processes are setup.

Noisebridge currently does not have the permits or processes necessary to handle hazardous waste generation and disposal. Therefore no chemicals or reagents requiring hazardous waste disposal should be brought into Noisebridge until such processes are setup.

Latest revision as of 05:37, 15 August 2010

Contents

No experimenting with or on any surface or plate/cup/container that can hold food.

Experiments and reagents should be stored in their own fridge. Do not place your experiments, media, or chemicals in the food fridge.

Don’t place your experiments near any food or drink.

Sterilize all waste as necessary, and dispose of all waste properly. Specific guidelines for waste disposal may be found below.

Disinfect/wipe down of any surface you've been working on with Lysol or ethanol.

Cover dishes if you aren't directly working with your culture (to keep from contaminating your culture, your peers and Noisebridge).

Know how to safely use and dispose of materials (reagents for example) before they enter Noisebridge. Do not bring reagents in which require hazardous waste disposal - Noisebridge currently does not have the permits or processes necessary for this.

Many materials used in wet biology are flammable or otherwise harmful and will be kept in a locked cabinet labeled with the appropriate warning labels (ie: flammable, skull and crossbones, etc.). Flammables, acids, and bases should be stored in a segregated manner.

Material Safety Data Sheets will be kept near the cabinet for all materials stored there.

Noisebridge is a humane space -- no animal experimentation!

Extra caution must be used in dealing with chemicals and cell cultures for not just healthy adults, but also children, elderly, immunosuppressed people, people with high allergen reactions, pets, and anything the pets may ingest including roaches/mice/pests that may reside at Noisebridge. All of these things come into Noisebridge and we can by NO MEANS be thought of as lab environment (until we come up with dedicated space for that).

All waste that has come in contact with bacteria, yeast, or transgenic cells is considered biohazard waste. Biohazard waste must be sterilized before being disposed of. The following two methods of sterilization may be used:

Noisebridge currently does not have the permits or processes necessary to handle hazardous waste generation and disposal. Therefore no chemicals or reagents requiring hazardous waste disposal should be brought into Noisebridge until such processes are setup.