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1%ˈ260nm ratio)/ E1cm Protective effects of compounds on TMV in vivo. The compound solution was smeared on the left side while solvent was served as control on the right side of growing Nicotiana tabacum. L leaves of the same ages. The leaves were then inoculated with the virus after 12 hours. A brush was dipped in tobacco mosaic virus of 6h10ˉ3mg/mL to inoculate the leaves, which were previously scattered with silicon carbide. The leaves were then washed with water and rubbed softly 12 Environment-Friendly Antiviral Agents for Plants along the nervature once or twice.

10(15 mmol) was added to ammonium hydroxide (30ˁ, 15 mL) and the solution was stirred for 1h at reflux. 11 was formed which was removed by filtration and dried. Dialkyl phosphite (6 mmol) was added to this solid and the resulting solution was stirred for 2 h at 70ć. 12 was produced in this process and then p-toluenesulfonic acid (6 mmol) in 50 mL tetrahydrofuran (THF) was added to the reaction mixture and stirred for 2h at 0ć. 13 was removed by filtration and washed with THF (20 mL). The precipitate was added to 30 mL aqueous ammonium hydroxide (10ˁ) and stirred for 30min at room temperature.

2% against TMV. To our knowledge, this is the first report on the syntheses and antiviral activity of chiral 3-[N-(substituentphenyl)amino]-3-(substituent-phenyl)-2-propenylphosphonates. 15 20 Environment-Friendly Antiviral Agents for Plants for their great biological and chemical relevance. Some derivatives of D-aminophosphonic acids are used as fungicide, plant virucide and herbicide. 28,58 ˉ 62 However, only a very few reports exsist on the incorporation of phosphonate moiety into the parent amide unit.