Datasheet Extract

Immunogen

Immunogen:

Synthetic peptide (C-YKVEIDTKSYWK) from the internal region of the protein sequence according to Human Prealbumin (NP_000362.1).

AA Sequence:

C-YKVEIDTKSYWK

Add. information

Western Blot:Preliminary experiments gave an approx 20-22kDa band in Human Heart, Kidney, Liver, Lung, Placenta and Testis lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 15.9kDa according to NP_000362.1. The 20-22kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Prealbumin is a serum protein usually occurring at 200-400mg/L serum. Prealbumin migrates faster than albumin in acidic starch gels and include alpha 1 antitrypsin, thyroxine binding prealbumin, and orosomucoid, an alpha 1 acid glycoprotein. The functions of prealbumin are to transport thyroxine, a task it shares with thyroxine binding globulin, retinol binding protein and vitamin A. Polymorphism of prealbumin is known in the mouse and pig.

Concentration

0.5 mg/ml

Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing. Shelf life: one year from despatch.