Affymetrix GeneChip Sample Submission Requirements

Affymetrix® GeneChip® Sample Submission Requirements

For submission of samples that will be labeled and arrayed, please submit RNA that has been resuspended in nuclease-free WATER ONLY in a volume of 5-8ul. For the minimum starting amount of RNA required please see the table below.

All samples will have a quality check before arrayed. Please provide an extra 3ul aliquot of each sample in a separate tube for this quality analysis.

***Please note: It is critical that the QC tube has the same concentration as the sample tube.***

Samples will be checked on NanoDrop for concentration and for the 260/280 and 260/230 absorbance ratios. Samples will also be checked using the Agilent Bioanalyzer for RNA integrity.

Fragmented and Labeled cDNA for GeneChip Hybridization
(NuGEN, all varieties)

Follow appropriate protocol

Follow appropriate protocol

>50 ul

>No

*Please submit 3ul of the ssDNA elution in one tube for QC (to ensure absence of contamination), and submit the remainder of the ssDNA elution (~27ul) in a separate tube for the fragmentation and labeling protocol. (See NuGEN WT-Ovation Pico System User Guide Step IV, I, 14-17, for elution information. Click here for product page.)