New Method For Bone-marrow-derived Liver Stem Cells Isolation And Proliferation

Date:

April 15, 2009

Source:

World Journal of Gastroenterology

Summary:

Bone-marrow-derived liver stem cells were once a hot topic in the field of stem cell research because of their important therapeutic implications, but little progress has been made in recent years because of the difficulty of isolation and proliferation of this special cell population. A research group in China has provided a new method for BDLSC isolation and proliferation, which brings new hope to the clinical use of bone-marrow-derived stem cells.

Share:

Total shares:

FULL STORY

Bone-marrow-derived liver stem cells were once a hot topic in the field of stem cell research because of their important therapeutic implications, but little progress has been made in recent years because of the difficulty of isolation and proliferation of this special cell population. A research group in China has provided a new method for BDLSC isolation and proliferation, which brings new hope to the clinical use of bone-marrow-derived stem cells.

Great interest has been aroused in the identification and isolation of liver stem cells from bone marrow cells. Several subsets of bone marrow cells have been found to have the potential to differentiate into hepatocytes, however, sorting based on immunological methods is difficult because of the complicated surface markers of the stem cells; furthermore, no report of successful passage has been published.

A research team led by Dr. Cai and his colleagues from the Affiliated Foshan Hospital and the Second Affiliated Hospital of Sun Yat-sen University established a carefully designed culture system to isolate, proliferate and differentiate liver stem cells directly from bone marrow cells, and they were able to achieve six passages of the stem cells. The results suggest that BDLSCs can be purified and passaged.

The selecting culture system that contains cholestatic serum can purify BDLSCs directly from bone marrow cells, which provides an easy method to separate stem cells, by avoiding complicated immunological manipulation. The successful passage of the stem cells further verifies the proliferating ability of the cells, although the passage is limited, and further research will provide more experience.

In this study, the authors used their original method to retrieve the cells, which are possibly BDLSCs. Then, they used fluorescence-activated cell sorting to determine the cells' characteristics before and after differentiation. This is an interesting and potentially important study, which suggests that bone-marrow-derived cells can be stimulated to expand and then differentiate into hepatocyte-like cells, which can possibly be used to treat liver disease.

World Journal of Gastroenterology. (2009, April 15). New Method For Bone-marrow-derived Liver Stem Cells Isolation And Proliferation. ScienceDaily. Retrieved August 2, 2015 from www.sciencedaily.com/releases/2009/04/090415102127.htm

July 31, 2015  School is just around the corner, which means backpacks and packed lunches await your children. One expert offers tips for parents to promote healthy dental habits while away from ... read more

July 29, 2015  By blocking the expression of a certain gene in patients, researchers have contributed to the demonstration of great decreases in the concentration of triglycerides in their ... read more

July 29, 2015  Viewing aquarium displays led to noticeable reductions in blood pressure and heart rate, a research team found in the first study of its kind. They also noted that higher numbers of fish helped to ... read more

July 16, 2015  Bone marrow contains stem cells that normally give rise to new red and white blood cells. A team of researchers from Sweden and France has now shown that bone marrow cells can also form ... read more

May 18, 2012  Placental stem cells with important therapeutic properties can be harvested in large quantities from the fetal side of human term placentas (called the chorion). The chorion is a part of the ... read more