Nucleophosmin (NPM, B23, Numatrin) is a phosphoprotein associated with cell nucleoli. Treatment of cells with cytotoxic drugs may make nucleophosmin enter nucleoplasm. It is reported that nucleophosmin shuttles continuously between cytoplasm and nucleoli to carry newly synthesized ribosomal proteins into nucleoli. NPMALK is a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2;5)(p23;q35) translocation found in anaplastic large cell lymphoma. In acute promyelocytic leukemia NPM gene is fused to RARalpha gene in (5;17) (q33;q12) transition.

Catalog #

N7100-15

Applications

Suitable for use in Immunohistochemistry. Other applications have not been tested.

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol 40-50%, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Clone Type

Monoclonal

Isotype

IgG/k

Clone No

3F291

Host

Mouse

Source

Human

Concentration

~0.2mg/ml

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See N7100-15X

Purity

Purified by Protein G affinity chromatography.

Immunogen

GST fusion protein containing the N-terminal part of nucleophosmin fused to 14 aa of ALK protein.1.

MW of Antigen

38kD. Epitope: N-terminal.

Cellular Localization

Nuclear

Specificity

Recognizes human Nucleophosmin (NPM, B23, Numatrin).

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.