Abstract: Objective: This study was carried out to investigate the effect of supplementing diets containing date palm fronds with cellulase and tannase enzymes. Materials and Methods: Fifteen lactating buffaloes were randomly assigned into three groups (five animals each) using complete random design and the experimental diets were T1 group fed 50% (CFM), 30% Egyptian clover and 20% rice straw, T2 group fed 50% (CFM), 20% clover, 15% rice straw and 15% date palm fronts and T3 group fed T2 diet plus 4 g kg1 DM of enzymes. Results: The results showed that DMI decreased in T2 compared with T1 and T3. However, enzymes supplementation significantly (p<0.05) increased DM, CP, EE, NFE, NDF, ADF and cellulose digestibility compared with T2. Also, T3 decreased (p<0.05) CP, CF, ADF and cellulose digestibility compared with T1. Blood plasma of animals fed T2 diets recorded the lowest (p<0.05) values for glucose, total protein and globulin compared with other groups. But, T2 group was insignificantly (p>0.05) decreased for albumin and urea compared with T3 group. While, there were no significant (p>0.05) differences for cholesterol, AST and ALT among groups. Milk yield and energy corrected milk were significantly (p<0.05) increased with enzymes supplementation to diet (T3) compared with T2. The increases of milk yield and energy corrected milk were 6.24 and 2.58%, respectively for T3 compared with T1. While, T2 decreased milk yield and energy corrected milk by 12.61 and 20.31% compared with T1. There were no differences (p>0.05) between experimental treatments in total solids. While, there were significant (p<0.05) decrease in fat and protein between T2 and other groups. Solids not fat and ash were significant (p<0.05) increase with T2 and other groups. Conclusion: It could be concluded that cellulase and tannase enzymes supplementation to diet could enhance the performance of lactating buffaloes.