Given the great potential of 2HG as a biomarker in the
diagnosis and management of glioma patients as well as the workup of an
undiagnosed mass, the capability of precise detection of 2HG in vivo is
extensively needed. In 1H-MRS, the 2HG signals are severely overlapped with
other metabolite signals. Specifically, because of the close proximity of the
2HG 2.25 ppm resonance to the GABA 2.29 ppm resonance, when the 2HG levels
are relatively low, 2HG estimation is elusive. Here we present 2HG
measurements without considerable GABA contamination in patients in vivo,
achieved by constant-TE triple-refocusing difference editing at 3T.