Custom Mouse Ascites Project (200 Balb/C Mice) Description

Background

Hybridoma or other tumor-like cells can be injected into the peritoneal cavity, the cells multiply and produce ascites fluid in its abdomen. Ascites fluid contains a high concentration of antibody making it inexpensive and easy to use. Ascites is commonly used as the source for antibody production from hybridoma cell lines. As an alternative to ascites roller bottle cell culture services are available for clients with specific antibody expression requirements.

This service requires hybridoma cells that were produced by Rockland or that are provided by the client. Multiple mouse strains are available upon request. Ascites projects have 3 phases, phase 1 is the scale up of tissue culture to obtain sufficient the required cells, and phase 2 includes the priming of the mice and the injection of the prepared cells, phase 3 is recovery of the ascites fluid. Purification services are available upon request.

Purity/Specifity

Ascites is typically produced in the Balb/c mouse strain. Other strains including nude or scid mice are availalble on request. Optimized methods, include the quantity and volume of injected hybridoma cells, are used to establish peritoneal soft tumors. Subsequently accumulation of ascetic fluid occurs and this fluid is harvested to recover the produced antibody and cells are removed. The ascites method generates high yields of antibody, ascites is frequently processed, purified or characterized depending on your particular requirements. Commonly protein A or protein G purification is requested to obtain a purified final antibody product. Assay development and reporter molecule conjugation services are also available upon request.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.