Schedule-dependent response of neuroblastoma cell lines to combinations of etoposide and cisplatin.

Meczes EL, Pearson AD, Austin CA, Tilby MJ - Br. J. Cancer (2002)

Bottom Line:
Results were analyzed using median effect and combination index analyses.In all schedules in which cisplatin was administered prior to etoposide a synergistic effect was observed.Conversely, an antagonistic effect was seen in all schedules where etoposide was administered before cisplatin.

fig1: Dose response curves for SHSY5Y cells exposed to scheduled exposures of cisplatin and etoposide. For each schedule, curves are shown for both drugs as single agents administered at appropriate time points together with the drug combination. Cells were seeded in 96-well plates and allowed to adhere for 24 h (time 0). Cells were exposed to either a mixture of cisplatin and etoposide for 24 h (D), or, cisplatin for 24 h (A, C, F), washed and further exposed to 4 h of etoposide (A), 24 h etoposide (C) or drug free medium for 24 h followed by 4 h of etoposide (F). Panels B, E and G show the reverse of schedules shown in A, C, and F respectively. The mean of at least three experiments are shown and error bars represent standard deviations (s.d.) from the mean. For schematics at the top of each panel, black rectangles represent cisplatin exposures, white rectangles represent etoposide exposures.

fig1: Dose response curves for SHSY5Y cells exposed to scheduled exposures of cisplatin and etoposide. For each schedule, curves are shown for both drugs as single agents administered at appropriate time points together with the drug combination. Cells were seeded in 96-well plates and allowed to adhere for 24 h (time 0). Cells were exposed to either a mixture of cisplatin and etoposide for 24 h (D), or, cisplatin for 24 h (A, C, F), washed and further exposed to 4 h of etoposide (A), 24 h etoposide (C) or drug free medium for 24 h followed by 4 h of etoposide (F). Panels B, E and G show the reverse of schedules shown in A, C, and F respectively. The mean of at least three experiments are shown and error bars represent standard deviations (s.d.) from the mean. For schematics at the top of each panel, black rectangles represent cisplatin exposures, white rectangles represent etoposide exposures.

Mentions:
Figure 1Figure 1

Bottom Line:
Results were analyzed using median effect and combination index analyses.In all schedules in which cisplatin was administered prior to etoposide a synergistic effect was observed.Conversely, an antagonistic effect was seen in all schedules where etoposide was administered before cisplatin.