Molecular Studies Of Vibrio Cholerae Strains Isolated From Local Outbreak

Abstract

Cholera caused by toxigenic Vibrio cholerae is a major public health
problem in developing countries. Epidemiology surveillance of cholera and
comparative analysis of strains collected during outbreaks has demonstrated
clonal diversity among epidemic strains and a continual emergence of new clones
of toxigenic V. cholerae. In the present study, the Random Amplified
polymorphic DNA (RAPD) technique was performed to study the clonal diversity
on twenty isolates of V cholerae and five isolates of V. harveyi (as a
comparison). Eighteen of V cholerae isolates belong to the Ogawa serotypes,
while two isolates were Inaba and 0139 Bengal serotypes, respectively. Fourteen
out of twenty random primers yielded clear and reproducible bands. From the
RAPD banding profiles, the polymorphism rates of bands were much higher
between V cholerae and V harveyi than those found among V cholerae isolates.
According to the dendrogram generated from the RAPDistance software program,