A lipase producing Acinetobacter
haemolyticus TA106 was isolated from healthy human skin of tribal
population. The maximum activity of 55 U/ml was observed after medium optimization
using the “one variable at a time” and the statistical approaches. The optimal
composition of the medium was determined as (% w/v or v/v): tryptone - 1, yeast
extract - 0.5, sodium chloride-1, olive oil-1, Tween - 80 1, manganese sulphate
- 5 mM, sucrose- 1, pH-7. It was found that maximum production occurred in late log
phase i.e. after 72 h and at 200 rpm. From factorial design and statistical
analysis, it was found that pH,
temperature, salt, inoculum density and aeration significantly affected the
lipase production. It was also noted that inoculum density of 3 % (v/v),
sucrose (1% w/v) and manganese sulphate(5 mM)
displayed maximum lipase activity of 55 U/ml by conventional as well as
statistical method. Optimization studies also indicated the increase in specific
activity from 0.2 U/mg to 6.7 U/mg.

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