Description:
The Arrestin family consists of four members: Arrestin 1 (visual Arrestin), Arrestin 2 (b-Arrestin 1), Arrestin 3 (b-Arrestin 2), and Arrestin 4 (cone Arrestin). While visual and cone Arrestins are found almost exclusively in the retina, b-Arrestins 1 and 2 are ubiquitously expressed, and were initially described as negative regulators of G protein-coupled receptor (GPCR) signaling. More recently, b-Arrestins have been determined to serve as scaffolds for various signaling pathways, including the MAPK cascades activating ERK2, p38a, and JNK3. These b-Arrestin scaffolds tie together the appropriate kinases in series, forming a discreet signaling module that localizes components to specific subcellular environments and facilitates greater kinase activation.

Application:Immunohistochemistry: 3-10ug/ml with the appropriate secondary reagents to detect b-Arrestin 2 in paraffin-embedded sections of normal human cortex, hippocampus, and caudate putamen. Optimal dilutions should be determined by the individual laboratory.

Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.