hUCMSC Medium

Human Umbilical Cord Mesenchymal Stem Cell Growth Medium consists of Human Umbilical Cord Mesenchymal Stem Cell Basal Medium, Fetal Bovine Serum and two supplementary factors. In comparison to traditional growth medium, which lacks the two supplementary factors, this product can support the human wharton's jelly derived umbilical cord mesenchymal stem cell to expand more rapidly, and proliferate more passages. The expression level of typical mesenchymal stem cell markers (such as CD29, CD105 and CD44) of cells in this growth medium were significantly higher than that in traditional growth medium, and these cells keep the ability of differentiating into osteoblast, adipocyte and chondrocyte.

Components:

Human Umbilical Cord Mesenchymal Stem Cell Basal Medium 450 mL

(Cat. No. GSB9001-1)

Fetal Bovine Serum 50 mL

(Cat. No. GSB9001-2)

Supplementary Factor Ⅰ500 μL

(Cat. No. GSB9001-3)

Supplementary Factor Ⅱ 50 μL

(Cat. No. GSB9001-4)

Instructions:

Preparation of Complete Medium

1.Prior to use, thaw the Fetal Bovine Serum under refrigeration (2 to 8°C) until completely thawed.

Note: Flocculent precipitates may occur after thawing, which is normal, and does not affect the usage of fetal bovine serum.

Note: Prior to use, the complete medium should be warmed at room temperature for 30 to 60 min and protect from light, avoid warming it in 37°C.

Storage:

The Human Umbilical Cord Mesenchymal Stem Cell Basal Medium can be stored for up to one year at 2-8°C. Fetal Bovine Serum and Supplementary FactorⅠis stable at -20°C for up to two years, Supplementary FactorⅡis stable at -20°C for up to one year, repeated warming/cooling and freeze-thawing should be avoided. The exact expiration date was labeled on the bottles.

Once prepared, the complete medium should be stored at 2 to 8°C in dark and used in one month.

For typical mesenchymal stem cell markers, such as CD29，CD105 and CD44, the expression level of these markers in which cells grow in present Human Umbilical Cord Mesenchymal Stem Cell Growth Medium(Cat.No.GSB9001) were significantly higher than that in traditional growth medium(Figure 3)