%0 Journal Article
%A HOMMA, MICHIO
%A KANBE, TOSHIO
%A CHIBANA, HIROJI
%A TANAKA, KENJI
%T Detection of intracellular forms of secretory aspartic proteinase in Candida albicans
%D 1992
%J Microbiology,
%V 138
%N 3
%P 627-633
%@ 1350-0872
%R https://doi.org/10.1099/00221287-138-3-627
%I Microbiology Society,
%X Summary: The extracellular proteinase (EPR) of Candida albicans was induced in a medium containing bovine serum albumin as sole nitrogen source. There were two intracellular forms in cells induced to produce EPR, a 43 kDa protein (EPR) and a 45 kDa protein (cross-reacting material of EPR; CRM-EPR); these were detected by immunoblotting using anti-EPR antiserum. The 43 kDa protein (EPR) may be the same as the extracellular form judging by molecular mass, and the 45 kDa protein (CRM-EPR) may be a precursor form of EPR. Many dense granules were observed by electron microscopy near the plasma membrane of the mother cells in EPR-producing cells. Both the 43 and 45 kDa proteins were recovered in a membrane fraction and were solubilized by Triton X-100. When the membrane fraction was further fractionated by sucrose density gradient centrifugation, the 43 and 45 kDa proteins were differentially fractionated. This suggests that they were located in different membrane-bound structures and is consistent with an assumption that the 45 kDa protein is a precursor for EPR.
%U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-138-3-627