Specially about rbcS (nuclear genome gene) and rbcL (chloroplast genome gene) encoding the small and large subunit of Rubisco, respectively.

rbcS part:

Based upon the previous EST database, it is planned to determine the copy number and sequences of rbcS in the progenitor diploid cotton (A2 and D5 genome) and their tetraploid offsprings. Meanwhile, we will analyze the expression pattern of these A- and D-origin copies in the allotetraploid.

rbcL part:

We will estimate changes of A2-origin rbcL in aspects of its copy number and expression using maternal-parent diploid and tetraploid cotton.