Description:
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Two transcripts encoding different isoforms have been identified.

Applications: Suitable for use in ELISA, Western Blotting. Other applications not tested.

Recommended Dilution:ELISA: 1:50,000-1:65,000Western Blotting: 1:1000-1:2000Optimal dilutions to be determined by the researcher.

Storage and Stability:May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type

Isotype

Clone

Grade

Pab

IgG

Affinity Purified

Size

Storage

Shipping

Source

Host

100ug

-20°C

Blue Ice

Human

Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide, C-KNFEKLKKPRGETK, representing the C Terminus of the sequence according to NP_006254.1.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in tris-saline [pH7.3] with 0.5% BSA, 0.02% sodium azide.