≥95% (SDS-PAGE, Western blot). No other protease contaminants detectable.

Specific Activity:

~10U/mg protein. One unit is defined as the amount of enzyme that produces 1µmol p-nitroaniline or 7-amino-4-methylcoumarin per min. at 37°C in 100mM TRIS-HCl, pH 8.5, using H-Gly-Pro-pNA or H-Gly-Pro-AMC as a substrate respectively (enzyme dilution >1:500; incubation time 10-30 min.).

Shipping:

Shipped on Dry Ice

Short Term Storage:

-20°C

Long Term Storage:

-80°C

Use/Stability:

Stable for up to 3 months when stored at -20°C.

Handling:

Avoid freeze/thaw cycles.

Technical Info/Product Notes:

Inhibitors: Very sensitive to diisopropyl fluorophosphate (DFP), less sensitive to other serine enzyme inhibitors such as phenylmethylsulfonyl fluoride (PMSF) (Prod. No. ALX-270-184) and diethyl 4-nitrophenylphosphate (E600). Most of the usual sulfhydryl reagents have little or no effect, nor do chelating agents.