corboy at utdallas.edu wrote:
>> Seeking knowledge of the effects of glutathion in bacterial media. The
> purpose is analagous to adding 2-ME or DTT to solutions when purifying a
> protein by standard methods. My protein is secreted into the medium, and
> I am trying to maximize the availability of a single free Cysteine SH
> group by protecting it (anti-oxidant). To my knowledge, you cannot use
> 2-ME or DTT in culture because they are membrane permeable and toxic. As
> far as I know, glutathion is not membrane permeable, but can be imported
> by bacteria. What I want to know is how much glutathion can I add to
> bacteria growing in liquid culture to provide SH protection, but not
> affect growth or reduce existing disulfide bonds, like you would use 2-ME
> for in purification. Thanks in advance for any help I may get.
>> Mike Corboy
> University of Texas at Dallas
Ever thought of adding cysteine to the medium?
Achim