Notch Reporter Assay Kit (cAMP/PKA Pathway)

Notch Pathway Reporter kit is designed for monitoring the activity of Notch signaling pathway in the cultured cells. The kit contains the transfection-ready Notch1 NICD expression vector and CSL (CBF1/RBP-J) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized CSL responsive element upstream of a minimal promoter. The CSL (CBF1/RBP-J) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The noninducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and back.

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