Fixation:

Sections:

Staining Procedures:

Cover sections with Alizarin Red S (#26206-01) and follow the staining by transmitted light, on the stage of a staining microscope. Calcium sites will be covered with a vivid orange-red calcium-alizarin lake within a period of 30 seconds to 5 minutes. The end point should be chosen when the deposit is heavy but not too diffused.

Shake off excess stain and blot carefully with filter paper or alternatively drain very thoroughly.

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Electron Microscopy Sciences specializes in the manufacturing, preparation and distribution
of the highest quality laboratory chemicals and microscopy supplies and equipment
for electron microscopy, light microscopy, and histology.