This protocol describes a straightforward method to generate specific mutations in the genome of strictly lytic phages. Briefly, a targeting CRISPR-Cas9 system and a repair template suited for homologous recombination are provided inside a bacterial ...

Alex Sem

Phagelux Canada

Sylvain Moineau

Université Laval

Thanks for your comments. Indeed the protocol needs to be adapted to other hosts. Others have been very successful in adapting it to E. coli. Pseudomonas, etc. So I am not sure what are the issues. Please do not hesitate to let us know !

2018-12-18 13:06:21

Marie-Laurence Lemay

Hi Alex,Thanks for your comment. Could you share the most significant changes? This might help other readers.