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Title: Development and application of automated systems for plasmid-based functional proteomics to improve syntheitc biology of engineered industrial microbes for high level expression of proteases for biofertilizer production

Technical Abstract: In addition to microarray technology, which provides a robust method to study protein function in a rapid, economical, and proteome-wide fashion, plasmid-based functional proteomics is an important technology for rapidly obtaining large quantities of protein and determining protein function across an entire genome (Lefkovits et al., 2001; Norais et al., 2001; Shaw et al., 2002; Baglioni et al., 2003; Ho et al., 2004; Saad et al., 2005). It also offers the possibility of modifying proteins to optimize their function. This technology centers on the production of full-length gene libraries synthetically as a source of plasmid-based clones to express the desired protein in active form for determination of its function. These clones can be used in mutagenesis strategies for gene open reading frame (ORF) optimization to develop improved strains and cell lines for various commercial enzyme uses.