Interpretive Summary: Horse gram seeds contain various amounts of health promoting chemicals and nutrients for use as a functional food crop in hunger stricken and nutrient deprived people. Horse gram samples conserved at the USDA, ARS repository were evaluated for fatty acids, flavonols, and minerals. Several fatty acids, flavonols, and mineral concentrations varied among seven horse gram accessions. Several of these horse gram accessions could be used as a health vegetable in the southern U.S.A. and could be used in breeding programs to develop superior varieties containing specific concentrations of fatty acids, flavonols, and minerals.