TF Activation Profiling Plate Array II

Characterization

Description:

Transcription factors (TFs) are a group of cellular proteins that play essential roles in regulating gene expression. Often, a specific cellular signal pathway can activate multiple TFs and the expression of a specific gene is under control of multiple TFs. Therefore, monitoring the activation of multiple TFs simultaneously is very important. Different from TF Activation Profiling Plate Array I, Signosis’ TF activation profiling plate array II is used for monitoring 96 different TFs including NFkB, AP1, HIF1, and p53. Two 96-well plates are provided for the comparison of two samples.

Applicable Grid:

List of Applicable TFs:

1

2

3

4

5

6

7

8

9

10

11

12

A

AP1

CDP

GATA

NF-1

Pit

Stat3

XBP

FOXG1

HoxA-5

NRF2(A)

ProX1

SOX2

B

AP2

CREB

GR/PR

NFAT

PPAR

Stat4

AP3

FOXO1

HSF

Oct-1

RB

SOX9

C

AR

E2F-1

HIF

NF-E2

PXR

Stat5

AP4

FREAC-2

KLF4

Pax2

RUNX

SOX18

D

ATF2

EGR

HNF4

NFkB

SMAD

Stat6

COUP-TF

Gli-1

MyoD

Pax3

ROR(RZR)

SRY

E

Brn-3

ER

IRF

OCT4

Sp1

TCF/LEF

ELK

Gfi-1

MZF

Pax8

RXR

TFE3

F

C\EBP

Ets

MEF2

p53

SRF

YY1

FOXA1

HEN (NSCL-1)

Nkx2-5

PIT1

SF-1

USF-1

G

CAR

FAST-1

Myb

Pax-5

SATB1

TR

FOXC1

HNF-1

Nkx3-2

PLAG1

SMUC

VDR

H

CBF

GAS/ISRE

Myc-Max

Pbx1

Stat1

TFIID

FOXD3

HOX4C

NRF1

MEF1

Snail

WT1

Principle

Signosis’ TF Activation Profiling Arrays are used for monitoring the activities of multiple TFs simultaneously. In the technology, a series of biotin-labeled probes are made based on the consensus sequences of TF DNA-binding sites. When the probe mixtures incubate with nuclear extracts, individual probes will find their corresponding TFs and form TF/probe complexes, which can be easily separated from free probes through a simple spin column purification. The bound probes are then detached from the complexes and analyzed through hybridization with a plate, which each well is specifically pre-coated with complementary sequences of the probes.

Data

TF activation profiling plate array II analysis the activities of 96 TFs. HeLa cells were treated with and without PMA. The nuclear extracts were prepared and subjected to TF activation profiling plate array.