Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

Flow Cyt

Use at an assay dependent concentration.

ab37382 - Chicken polyclonal IgY, is suitable for use as an isotype control with this antibody.

WB

1/2000. Detects a band of approximately 49 kDa.

Target

Relevance

The Fc region is the constant region on an immunoglobulin molecule, the area that is exactly the same on all antibodies. The Fc region is found on the heavy chains and is not involved in binding antigens.

This product will recognize the Fab fragment of any exposed IgG. However if whole IgG is present and not masked by absorption into the substrate, these too will be recognized. I would recommend performing a co stain with an anti- IgG Fc specific antibo...

Thank you for contacting Abcam.
After consulting with my colleagues we think that ab15866 may be a suitable control for your experiments. Also I have attached a paper that looks at protein stains vs antibodies as loading controls, I thought it ...

Thank you for your phone call last week requesting information on antibody ab15866 raised against IgG Fc. I am sorry it has taken a while to source the information for you. It is likely that this antibody will recognise IgG4 Fc a as there is a 79% homo...

Thank you for your enquiry.
Further to correspondence with the lab I can tell you that unfortunately ab15866 has not been tested for the presence of endotoxins and are not sterile.
I am sorry that I cannot be of more assistance on this occasion.

Abcam Scientific Support

Answered on Jul 20 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"