Preparation Note

Always ensure that components have been fully thawed and thoroughly mixed before use. KAPA SYBR FAST qPCR Master Mix (2X) may not freeze solidly, even when stored at -20°C.

The SYBR Green I dye contained in KAPA SYBR FAST qPCR Master Mix (2X) ABI PRISM® and ROX/fluorescein dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.

KAPA SYBR FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week and may be stored at this temperature for short-term use, provided that they do not become contaminated with microbes and/or nucleases.

Quality

All kit components are subjected to stringent functional quality control, are free of detectable contaminating exo and endonuclease activity, and meet strict requirements with respect to DNA contamination.

Complete real-time PCR runs in just 40 minutes• 55% shorter run times with fast cycling protocol.• Maintain high performance when switching from standard to fast protocols.

Quick Notes:• This kit contains an engineered enzyme optimized for qPCR using SYBR Green I dye chemistry.• The 2X master mix contains a proprietary buffer. Together with the novel enzyme, this improves amplification efficiency of both GC- and AT-rich targets.• 20 sec initial denaturation at 95°C is sufficient for enzyme activation. When working with complex templates, an initial denaturation of 3 min is recommended.• For 3-step cycling, use 20 sec for primer annealing and 1 sec for extension/data acquisition at 72°C.• Do not exceed 25 μL reaction volumes.

Safety Information

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