Culture of Selected Microalgae in Rubber and Palm Oil Mill Effluents and their Use in the Production of Enriched Rotifers

Abstract

Three freshwater microalgae Chlorella vulgaris, Selellastrum gracile and
Ankistrodesmus convolutlls showed high growth performances in terms of specific
growth rate (SGR) of cell and chlorophyll a, total carotene and observed yield when
grown in inorganic media with sodium acetate. Total protein of S. gracile was higher
than A. convolutus followed by C. vulgaris, whereas reverse trend was observed in the
case of total lipid. Among 11 essential amino acids (EAAs), phenylalanine, leucine
and lysine were found to be higher in C. vulgaris, S. gracile and A. convolutus than
other EAAS. All the evaluated polyunsaturated fatty acids (PUFAs) of CIS, C20 and
C22 were higher in C. vulgaris grown in Bold Basal Medium with sodium (Na) acetate
(BBMAC) and Modified Medium with Na acetate (MMAC) than cultured in other
media. In addition, S. gracile and A. convolutus grown in BBMAC and MMAC
contained significantly (p<0.05) higher amount of PUFAs except linolenic acid ( 18:3n-3 ) and eicosatrienoic acid (20: 3n-6) than cultured in other media. All together 26
essential minerals were detected and 22 essential minerals were quantified where total
bioaccumulated minerals was below the optimum level and significantly (p <0.01)
correlated with these minerals available in media.