Fasting serum insulin levels in C57BL/6J (lean) and C57Bl/6J-Lepob (obese) mice were determined before (day 0) and after (day 5) exposure to either IH or IA for 5 days

These results are presented as the means ± s.e.m. The statistical significance of the difference between day 0 and day 5 within each group of animals or between animals exposed to intermittent hypoxia or intermittent air was derived with paired or unpaired t tests, respectively. * P < 0.01 for difference between day 0 and day 5; †P < 0.01 between mice exposed to IH and IA on day 5.

Blood glucose levels as a result of an IPGTT after a 5 day exposure to either IH or IA in lean mice, leptin-deficient obese mice and leptin-deficient obese mice receiving supplemental leptin

The change in the blood glucose from fasting levels was determined over a 2 h period after I.P. injection of glucose at 0.5 g kg−1 body weight in lean C57BL/6J mice (A), leptin-deficient obese C57Bl/6J-Lepob mice (B) and in C57Bl/6J-Lepob (obese) mice receiving leptin subcutaneously at 200 μg kg−1 day−1 (C). The IPGTT was performed after exposure to IH or IA for 5 days. The results show the mean of the change in blood glucose level (blood glucose level –fasting blood glucose level) ± s.e.m. The statistical significance of the difference between groups was derived with the method of generalized estimating equations (see Methods for the details). * P < 0.05 for the difference between groups.

Serum insulin levels as a result of an IPGTT after exposure to either IH or IA in lean mice, leptin-deficient obese mice and leptin-deficient obese mice receiving supplemental leptin

Serum insulin levels in C57BL/6J (lean) and C57Bl/6J-Lepob (obese) mice were determined before (fasting level) and 2 h after I.P. glucose injection (0.5 g kg−1 body weight) in the IPGTT. The IPGTT was performed after exposure to either IH or IA for 5 days. Results are presented as the means ± s.e.m. The statistical significance of the difference between fasting and 2 h IPGTT levels within each group of animals or between animals exposed to intermittent hypoxia or intermittent air was derived with paired or unpaired t tests, respectively. * P < 0.001 for difference between fasting and 2 h IPGTT levels; †P < 0.01 between mice exposed to IH and IA.

Fasting serum insulin levels in obese mice after exposure to either IH or IA for 12 weeks

Fasting serum insulin levels in C57Bl/6J-Lepob (obese) mice were determined before (baseline), during (weeks 4 and 8) and after exposure to IH or IA conditions for 12 weeks. Results are presented as the means ± s.e.m. Statistical significance of the difference between groups and over time was determined by two-way ANOVA. * P < 0.01 for difference between baseline, week 8 and week 12 levels for the IH group.

Blood glucose levels as a result of an IPGTT after a 12 week exposure to either IH or IA in obese mice

The change in the blood glucose from fasting levels was determined over a 2 h period after I.P. injection of glucose at 0.5 g kg−1 body weight in C57Bl/6J-Lepob (obese) mice following 12 weeks of exposure to IH and IA. Results are presented as the means of the change in blood glucose level (blood glucose level –fasting blood glucose level) ± s.e.m. The statistical significance of the difference between groups was derived with the method of generalized estimating equations (see Methods for the details). * P = 0.002 for the difference between groups, †P < 0.001 for the difference between groups.