Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

plasmacytoma; myeloma

Strain

BALB/c

Applications

Kappa chains are synthesized but not secreted.

Tested and found negative for ectromelia virus (mousepox).

The cells are resistant to 0.1 mM 8-azaguanine and do not grow in HAT medium.

The cells are resistant to 0.1 mM 8-azaguanine and do not grow in HAT medium.

The cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 105 viable cells/mL. Maintain cell density between 1 X 105 and 1 X 106 viable cells/mL. Do not allow the cell density to exceed 1 X 106 cells/mL.

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.