A survey of the bee fauna of Araucaria Forest was performed in the border of a fragment in the district of Porto União, in Santa Catarina State. Samples were carried on monthly between October/2005 and October/2006 with hand nets directly on the flowers, besides three types of traps: chemical baits, trap nests and pantraps. A total of 1711 bees were captured belonging to 164 species, distributed in five families of bees that occur in Brazil. From the total 1339 bees of 130 species were sampled with the hand net, 346 individuals of 72 species were collected in pantraps, 24 bees of nine species were caught in the trap nests and two specimens of one species were sampled with baits. At most 48 species were common to two or more sample methods. Dialictus, with 27 species, was the most representative genus in the area. The exotic Apis mellifera L. was the most abundant species, representing 49.6% of the sampled bees, following by Dialictus sp.11 with 3.2%. The results show evidences of a seasonal pattern of activity of the bees and the importance of the use of complementary methods to survey the fauna. The composition of the bee fauna was similar to many inventories performed in the Araucaria Forest, being Halictidae the most diverse family followed by Apidae.