Bismuth
Stain Kit for Specific En Bloc Staining

EMS #11430

Procedures

Staining solution:

Mix Solution A and B together (One part of A into two parts of B) then adjust to pH 7.0 with 1N HCl.

Aldehyde-fixed tissue blocks are rinsed in the above buffer and stained en-bloc with Bismuth for one hour at room temperature on a gentle rotary shaker. Postfixation is carried out with 1% Osmium Tetroxide in 0.05M Sodium Cacodylate buffer, pH 7.2.

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Electron Microscopy Sciences specializes in the manufacturing, preparation and distribution
of the highest quality laboratory chemicals and microscopy supplies and equipment
for electron microscopy, light microscopy, and histology.