Advantagesmuch higher resolutionmore control in the degree of magnificationlarger depth of field

Polymer sample preparation for SEMsputter coatingspin coatingstaining

Applications PAA in ethanol with 4 wt% concentrationEthylene glycol at the concentration of 16 wt% relative to the polymerThe solution was completely dissolved with a magnetic bar for 24 hours Right before electrospinning process, 1M sulfuric acid was added to the PAA solution at a concentration of 50µL/mLSetting: Distance: 20cmVoltage: 15kVFlow rate: 0.8mL/hVolume: 4mL PAA nanofiber scaffold was placed in a vacuum oven at 130°C and 30 in Hg of vacuum for 30 minutes

Then the scaffold was neutralized by placing in a base solution containing 1M NaOH and 1M NaCl for approximately one hour and then rinsing with water to remove residual salts 1 mg/mL solution of chlorhexidine prepared (completely dissolved)

Small sample of PAA scaffold with a diameter of 3mm soaked in the solution for 1 hour The sample was completely dehydratedSmall pieces of the sample were mounted on the standard SEM holder with the help of conductive carbon tapesThen, the samples were sputter-coated with a thin layer of goldPAA nanofibers were observed using SEM with an operating voltage of 15kVData treatment using free software ImageJ SEM sample holder* *http://sims.ess.ucla.edu/resources/SAMPLEPREP.php Fibers with uniform diameter (no bead structures) with an average diameter of 860 ± 40 nmNo chlorhexidine aggregates or crystals observed on the surface of the fibers Addition of drugs or other molecules at low enough concentrations will not have an adverse effect on fiber morphology - Thank you - Si Young An4/24/13