Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.

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Cells were fixed with 4% formaldehyde in PEM buffer. The coverslip was incubated in blocking buffer of 5% powdered milk in TBS-T plus 0.02% sodium azide for 1 hour at room temperature. Blocking buffer was removed and primary antibody was added at a dilution of 1/200 and incubated overnight at 4 degrees celsius. The coverslips were then washed 4-5 times with blocking buffer for 5 minutes. Secondary antibody, goat anti-rabbit Alexa 594 (ab150080), was added at a dilution of 1/1000 and incubated at room temperature for one hour. From this point on coverslips were covered with foil to protect them from light. They were washed 5 times with TBS-T and then one time with PEM, for 5 minutes each wash. The coverslips were fixed 10-30 minutes in 4% formaldehyde in PEM buffer, then washed 3 times with PEM buffer for 5 minutes. 0.1M ammonium chloride in PEM buffer was added for 10 minutes to quench auto-florescence, and then slips were washed 2 times for 5 minutes in PEM followed by 3 washes for 5 minutes in

I can confirm that both ab2790 and ab2787 antibodies are sold as ascites fluid. As discussed on the phone, unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet.

Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for ascites, concentration of antibody is known to very between 5 - 10 mg/ml.

I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

I can confirm that these two products are the same clone (therefore the clone number G3.1 is identical) so the immunogen sequence used to raise them was exactly the same. However, ab2790 is conjugated whilst ab115639 labelled to DyLight® 488.

If you need any further assistance in the future, please do not hesitate to contact me.

Thank you for your enquiry.
I can confirm that this product has been successfully used in IHC in the following article.
Ye H et al. Proteomic based identification of manganese superoxide dismutase 2 (SOD2) as a metastasis marker for oral squamous cell carcinoma. Cancer Genomics Proteomics 5:85-94 (2008). WB, IHC-P; Human. PubMed: 18460737
http://www.ncbi.nlm.nih.gov/pubmed/18460737?dopt=Abstract
Unfortunately, I was not able to locate the data demonstrating reactivity with mouse HSP27 at this time. Therefore, I will temporarily change the mouse reactivity from the list of approved species to "predicted to react" until we can produce the required data.
However, I have run a sequence align and found that the mouse HSP27 sequence is strongly conserved within the human sequence (see attached file). Also, this is one of our oldest clones, and I there are no customer complaints for using this antibody in mouse. Therefore, we expect that it will work for your too.

Thank you for your enquiry.
Regarding the HSP-27 antibody (ab2790), a sodium citrate heat mediated antigen retrieval method has been used with this product in IHC techniques (I have added this information to the datasheet for future reference). I would recommend adding a permeabilization step, such as Triton X-100 or saponin, as well.
I am still looking into ab13494 for you, and will email again once I have more information.