smallRNA / miRNA Seq Analysis Service

Low amounts, short turnaround time, PCR bias elimination

We use our gel-free single tube protocol for high quality smallRNA library preparation. Our technology (sample preparation and “TrueQuant“) effectively reduces ligation-bias and PCR-bias observed in other protocols. Our TrueQuant PCR bias elimnation is especially useful for smallRNA analysis, as the number of different molecules is limited.

Only 0.1 ng of total RNA is required as input material for our smallRNA Seq service.

Bioinformatics

Required Material: 1 µg of total RNA including microRNAs (please inquire for appropriate protocol) or appropriate amount of sample tissue

Low amounts of input material ?

We have developed an optimzed protocol for low amounts of material. Because we can eliminate PCR-bias thanks to our “TrueQuant” method, the data remains more accurate even at higher numbers of PCR cycles.

Required material: 0.1-100 ng of total RNA or the corresponding amount of fresh material. Please contact us for further information at info@genxpro.de or give us a call at +49 69 95739710.