NOTICES OF AVAILABILITY (RFPs AND RFAs)
ONCOGENE ANALYSIS FOR MOLECULAR TOXICOLOGY STUDIES
NIH GUIDE, Volume 21, Number 12, March 27, 1992
RFP AVAILABLE: NIH-ES-92-25
P.T. 34
Keywords:
Toxicology
Genetics
Oncology
Nucleic Acid Sequencing
National Institute of Environmental Health Sciences
The primary purpose of this project will be to provide laboratory
support by the application of assays to screen for and verify mutations
in oncogenes, such as ras or p53, in samples of frozen or fixed tissues
using a variety of molecular techniques. Specific methods to be in
place include polymerase chain reaction (PCR) amplification,
oligonucleotide hybridization, restriction fragment length polymorphism
(RFLP) analysis, direct sequencing of PCR products, and single stranded
conformation polymorphism (SSCP). Specific tasks include, but are not
limited to, DNA extraction, amplification of regions of p53 or other
genes, screening for mutational activities of ras or other genes or
mutational deactivation of tumor suppressor genes, and assay for loss
of heterozygosity. All responsible sources may submit a proposal that
will be considered. The Request for Proposals (RFP) for this estimated
five-year competitive procurement will be released on or about March
27, 1992. Proposals will be approximately 40 days thereafter. The
institute expects to made one award from this solicitation.
Requests must reference RFP NIH-ES-92-25 and must be forwarded to:
National Institute of Environmental Health Sciences
Contracts and Procurement Management Branch
ATTN: Thomas M. Hardee, Contracting Officer
79 T.W. Alexander Drive, 4401 Building
P. O. Box 12874
Research Triangle Park, NC 27709
Telephone: (919) 541-7893
.