- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

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制备和贮存

稳定性和存储

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Information available upon request.

常规信息

别名

Cit Histone H3

Citrullated

citrullin

Citrullinated Histone H3

citrulline

H3 3 like sequence MH921

H3 3A

H3 histone

H3 histone family member E pseudogene

H3F3

HIST3H3

Histone H3 3 pseudogene

Histone H3 Citrullated

Histone H3.1

Histone H3.1t

Histone H3.2

Histone H3.3

Histone H3.3C

Histone H3.5

see all

相关性

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- modifications of histones, also called histone code, and nucleosome remodeling.
P68431 and Q71DI3 are expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
Q16695 is expressed in tesicular cells.
Q6NXT2 is specifically expressed in the seminiferous tubules of testis and this Hominid-specific H3.5/H3F3C preferentially colocalizes with euchromatin, and it is associated with actively transcribed genes.
P84243 is a variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin.
Post-translational modification.
Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.