NIK AntibodyCATALOG NUMBER: 1129

Immunocytochemistry of NIK in Hek293 cells with NIK antibody at 10 μg/mL.

Immunofluorescence of NIK in Hek293 cells with NIK antibody at 20 μg/mL.

Green: NIK Antibody (1129) Blue: DAPI staining

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0.02 mg - $79.00

0.1 mg - $335.00

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Additional Info

Background

Specifications

SPECIES REACTIVITY:

Human

HOMOLOGY:

Predicted species reactivity based on immunogen sequence: Mouse: (94%)

TESTED APPLICATIONS:

ELISA, ICC, IF, WB

APPLICATIONS:

NIK antibody can be used for detection of NIK by Western blot at 1 - 2 μg/mL.

Antibody validated: Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

USER NOTE:

Optimal dilutions for each application to be determined by the researcher.

POSITIVE CONTROL:

1) Cat. No. 1210 - HEK293 Cell Lysate

PREDICTED MOLECULAR WEIGHT:

Predicted: 104 kDa

IMMUNOGEN:

NIK antibody was raised against a 17 amino acid peptide near the carboxy terminus of human NIK.

The immunogen is located within the last 50 amino acids of NIK.

HOST SPECIES:

Rabbit

Properties

PURIFICATION:

NIK Antibody is affinity chromatography purified via peptide column.

PHYSICAL STATE:

Liquid

BUFFER:

NIK Antibody is supplied in PBS containing 0.02% sodium azide.

CONCENTRATION:

batch dependent

STORAGE CONDITIONS:

NIK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Background

BACKGROUND:

NIK Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, LPS and mitogens. A serine/threonine protein kinase which mediates NF-κB activation by IL-1, TNFα and CD95 was identified recently and designated NIK (for NF-κB inducing kinase). NIK is an activator of IκB kinase alpha and beta (IKKα and IKKβ). Therefore, NIK is a key molecule in the NF-κB signaling pathway leading to the induction of a variety of gene expression in response to proinflammatory cytokines and bacteria products.