Cultures were retrieved from incubation and minipreps were performed following the protocol. This was followed by a restriction enzyme digest reactions using Xba1 and Nde1 enzymes each relevant to its DNA .After incubation samples were run on a gel to confirm the results.

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Cultures were retrieved from incubation and minipreps were performed following the protocol ''fig 2''. This was followed by a restriction enzyme digest reactions using Xba1 and Nde1 enzymes each relevant to its DNA .After incubation samples were run on a gel ''fig 3''to confirm the results.

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Cultures were retrieved from incubation and minipreps were performed following the protocol fig 2. This was followed by a restriction enzyme digest reactions using Xba1 and Nde1 enzymes each relevant to its DNA .After incubation samples were run on a gel fig 3to confirm the results.