“This instructable was created in fulfillment of the project requirement of the Makecourse at the University of South Florida (www.makecourse.com)Sampling is an important aspect of almost any wetlab as they can be analyzed to provide important information for research, industry, etc. However the frequency of sampling can be tedious and require the frequent presence of someone to take said sample including weekends, holidays, etc. An autosampler can relieve such demand and eliminates the need for scheduling and maintaining a sampling schedule and the personnel to execute it. In this Instructable a demonstration autosampler was constructed as a simple system that can be easily constructed and operated. Please watch the linked video to glimpse an overview of the development of this project.The following is a list of the materials used to construct this project, all of these components should be able to be found in stores or online with a quick search:1 x 3-D printer1 x Hot Glue Gun3 x Screws1 x Screwdriver1 x Arduino Uno1 x Breadboard1 x USB to Arduino Cable1 x 12V, 1A Barrel Plug External Power Supply1 x 12V Peristaltic Pump w/Iduino Driver1 x Nema 17 Stepper Motor w/EasyDriver1 x Magnetic Reed Switch2 x Buttons1 x 25mL sample vial1 x 1.5” x 1.5” styrofoam block, hollowed outPin wires for connecting Arduino and breadboardCAD software (i.e. Fusion 360/AutoCAD)”

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