Used at the climax of episode 3 by Amy Rose, Sonic's self-appointed "girlfriend".

Shadow the Hedgehog does this in episode 61 while trying to defeat the Metarex ship.

The English dub of Kanon(2006) has the young Yuichi screaming "NOOOOOOOOOO!" after Ayu falls off a tree and apparently dies (she actually slips into a coma). In the Japanese version Yuichi instead screams "AYUUUUUUUUUU!"

Though the series engages in more and more towards the end, the biggest of Big Noes in Neon Genesis Evangelion are one by Asuka at the end of herMind Rape, one by Shinji after realizing his Mecha has been used by his father to crush the Entry Plug loaded with his unconscious best friend, another for Shinji after seeing that the MP-Evas have murdered and EATEN Asuka alive and one by Maya Ibuki (who spends most of End of Evangelion freaking out) after Giant Naked Rei's hand passes right through her entire body. Some of these were dubbed into English as inarticulate yells, probably to avoid being straight-up Narm.

Since the Japanese word usually used for the Big No, "iya," sounds like a scream anyway, it only makes sense to translate these as such.

Used in Nadia: The Secret of Blue Water by the title character, twice in the final episode first when Emperor Neo sacrifices his life to free her from Gargoyle's control, and, even more heartbreakingly, when Gargoyle kills Jean by making him fall to the floor. (Nadia eventually revives him with the Blue Water.)

In Digimon Adventure, Hikari does one when Wizardmon "dies", leading to the activation of her crest. It was strangely cut from the dub. Equally as strange, the clip was shown in a brief flashback in a later episode, except during a voiceover narration, so her voice was not heard.

Also used in the episode "Ogremon's Honor" by Mimi when Leomon "dies"note W Ith the exception of Savers, Digimon don't actually "die", exactly. They just get reconfigured. They are computer data, after all.

Ill Girl Hayate has a huge, primal one near the end of the second season. Considering how it punctuates a particularly nasty Break the Cutie moment, the overall effect is quite chilling.

In the fourth Megami sound stage, Shamal gives one in a more light-hearted moment when Agito inadvertently shows Hayate a cake from Nanoha's parents' cafe that was supposed to be a surprise (as part of celebrating the tenth anniversary of Hayate recovering) due to being unable to read the kanji on the box.

Fate has one in the one-shot "Second Mother's Day," when she worries that when Vivio doesn't give her a gift for Mother's day, she will soon stop seeing her as a mother and eventually no longer recognize her.

In a mid-series episode of Mobile Suit Gundam Wing, Duo gives one out when Trowa destroys his Gundamnote As part of an If You're So Evil, Eat This Kitten situation. It's even bigger in the US dub; voice actor Scott McNeil has said in order to achieve this, he thought about the time his wife ran over his motorcycle with the family minivan. It's actually so famous that he's often asked to re-enact it at conventions.

In Robotech: The Shadow Chronicles there are two moments - first when Commander Tailor dies and then when Alex Romero sacrifices himself. This one is especially strong.

Simon from Tengen Toppa Gurren Lagann performs a variation on this trope, instead shouting "ANIKI!!!" when he believes Kamina has been killed by Thymilph. Kamina, however, stays alive just long enough to make a Heroic Sacrifice in the form of the Giga Drill Breaker.

The king (or rather, queen) of this trope is - surprise, surprise - Kinon. When Rossiu is about to shoot himself in the head, Kinon screams out a teary DAAMEEEEEEEEEEE!!! which stops him dead in his tracks - just in time to receive a Get a Hold of Yourself, Man! punch from Simon.

The second Greed gets one of these after the revived and amnesiac Greed unknowingly kills the last living member of the former Greed's Quirky Miniboss Squad. Ling, whose body the current Greed is inhabiting, asks What The Hell Anti-Villain, causing the old Greed's memories to resurface, with the new one letting out a screen-filling, gut-wrenching, Big No.

Hohenheim pulls an inarticulate scream of despair and horror after he realizes he's the Last of His Kind (the last human alive in Xerxes, his country, to be exact) and has become a living philosopher's stone.

In chapter 107, Ed lets out a Big No when he realizes Al is sacrificing his own soul to restore Ed's real arm and save him from Father.

In anime episode 79 (the Bount arc), Uryu Ishida has one after Yoshino is killed.

Shinji does this in the flashback (Turn Back the Pendulum) arc.

In anime episode 217, Charlotte Cuuhlhourne does one just before he's killed by Yumichika's sword.

In anime episode 271, Orihime Inoue does one right after Ulquiorra fires a cero blast into Ichigo Kurosaki, leaving a huge hole in his chest and apparently killing him. In its original manga rendition (chapter 349), it spans two pages and she even needs to take a breath before continuing with it.

Toshiro Hitsugaya in chapter 392 when he realizes he's stabbed Momo and not Aizen in the chest.

Earlier in the same season (in the original and English dub), Bulma let out one upon learning that Vegeta had been killed by Buu.

Even earlier, in the dub only (he screams "Goku" in the original), Krillin uses one when it becomes clear that Goku is going to sacrifice himself to take Cell away.

In the dub, Trunks seemed to have mastered the Narm of the Big No, with lines like "If they let that android free, it will be the end of all of us! NOOOOOOOOOOOOOO!" (Lampshaded in this fanvid: http://www.youtube.com/watch?v=_HOAETv_nVo) and not too long after "FATHER! hurgh, NOOOOOO! FATH-ER!"

But Trunks also has a decidedly non-narmy Big No in the dub of his OVA when he finds Future Gohan dead. In the original Japanese, it's a simple Skyward Scream.

Earlier than the other times, Goku lets out one of the biggest Big Nos ever: after defeating Frieza on a crumbling Namek, Goku tries to escape on Frieza's ship, but unfortunately, the ship was damaged and plummets into a fissure to dissolve in lava, leaving our hero with no way to escape.

Goku uses instant transmission to do a point blank kamehameha on Cell here.

Let's not forget Jeice's TWO Big Nos for the price of one when he realizes Vegeta is going to kill him late in the Frieza Saga.

Sayonara, Zetsubou-Sensei subverts this by having one of the girls peep on their (male) teacher, and when they knock over the privacy wall and each side gets a good eye-full of the other, only he screams, much to his consternation.

Chitose utters these quite often when having to deal with his self-proclaimed mothers on Happy Lesson, but the entire Biology class gets one when the lesson of the day is: "Dissection - Live chicks".

Satoko plays this straightforward the anime-exclusive "Disaster Awakening" chapter of Higurashi: When They Cry when she sees the bodies of her schoolmates in the aftermath of a disastrous toxic gas leak that wiped out virtually the entire population of 2,000 people in her village other than herself.

In the first InuYasha movie (both the English and Japanese versions) Kagome lets out a rather chilling one after being forced to shoot Inuyasha by the Big Bad Complete with lack of BGM and echoes. It is used rather effectively though.

In History's Strongest Disciple Kenichi, during a financial crisis, Miu has the masters of Ryouzanpaku give lessons to small children. Kenichi is amused to see the straight-faced (but cruel) Akisame being run ragged by the hyperactive kiddies, but enacts this trope when he later sees what kind of effect his teachings have.

At the end of the first ep of Nyan Koi!, Junpei lets out one in its full Engrish glory.

In the manga version of Prétear, Himeno lets out a Big No when she realizes that her carelessness has led to Shin's life being drained away.

In K-On!, Mio lets out one of these after her on stage... accident. Involving a "bowl of rice".

The sixth volume of the King of Thorn manga devotes a full page to a Big No by Shizuku when Kasumi dies.

Sekai yells this in the last anime episode, when Kotonoha and Makoto kiss in front of her.

In episode 10 Nanami becomes extremely upset after the School Festival, when a sex tape of her and her boyfriend is shown to many classmates as a cruel joke from Otome's Girl Posse. She lets out one of these before collapsing to the ground in tears.

In season 2, episode 6 of Girls Bravo, a cross-dressing Yukinari screams this at one point.

In chapter 52 of Future Diary Yukiteru does this when Yuno asks him to kill her.

In the final scene of the last episode of Monster we are treated to a flashback of Nina's and Johann's childhood where their mother is forced to give one of them up for an experiment. The two hold onto their mother's hand begging her not to let go, and their mother hesitates before handing over Nina who proceeds to scream "No!" in horror as tears spring from her eyes.

Gankutsuou: Victoria Danglars does this in the 8th episode before fainting.

Halo Legends: Specifically in The Duel, where Fal discovers the dead body of his wife and lets out his "NOOOOOOOOO".

His inflection makes it a bit difficult to tell, but it seems more like an inarticulate Skyward Scream.

Used in Karin (also called Chibi Vampire) in conjunction with Big "OMG!" when Winner sees his most recent test score. Link

In the first episode of Future GPX Cyber Formula ZERO, Asuka lets one out when she sees Hayato lying down on the ground with his car completely destroyed after his car crashed down from the track with him in it at the English Grand Prix.

In Air when Misuzu dies, her mother figure shouts out her name in this fashion ("MISUZUUUUUUUU!").

In Tokyo Mew Mew, Ichigo's boyfriend screams this when Ichigo is about to be kissed by Kisshu and Ichigo screams this as well before knocking Kisshu away.

Magic Knight Rayearth: Princess Emeraude releases one when Zagato dies in a battle with the Magic Knights.

Happens in a couple of Hinako Takanaga's works:

In The Tyrant Falls in Love, the homophobic Hot Scientist Souichi does this whenever his lab assistant Morinaga comes onto him. Matters are confused because he doesn't want to face up to his potential homosexuality and so pretends to resist Morinaga to satisfy his own guilt even when he secretly wants to have sex - the Big "NO!" comes out when he really doesn't want it and needs to make that clear.

In Little Butterfly Kojima pushes his boyfriend Nakahara off with this whenever a makeout session gets too intense and it looks like sex might happen. Needless to say, this happens quite a lot.

Card Captor Sakura: Meiling lets out one when Syaoran is injured by The Shot, which she accidentally released.

In Naruto Izumo yells this in his mind a few seconds before Hidan kills Asuma.

During the traumatic end of the first episode of Attack on Titan, Eren Yeager lets loose with a big "YAMEROOOOOOOOOO!"note "STOP IT!" as his mother is about to get eaten. In the manga and the English dub, this scene is served with a proper Big "NO!".

In YuYu Hakusho, when Kuwabara is struck down by Toguro, Yusuke lets one loose.

Prior to that, when Genkai told him that allowing Kuwabara to die was the only way to beat Toguro, Yusuke belted one at her before calling her out.

In the first episode of Is the Order a Rabbit?, Tippy does one when Cocoa is holding him too long. At first she's confused by it, but Chino tries to play it off as ventriloquism. However, Tippy is later revealed to be able to talk.

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