4) Freeze with liquid nitrogen and place in freezer at -20°C for 24 hours

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==Observations==

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*FCS measurements were taken of the the 0.02% beads as well as solutions 2 and 5. According to our measurements, there were no spheres present in solutions 2 or 5. Because of this, we are going to prepare new sets of hydrogels (Part 4) in which we do not allow them to dry after filtration. We will instead immediately place them back into solution. We are hopeful that this will allow the hydrogels to stay separate instead of aggregating.

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4) Freeze with liquid nitrogen and place in freezer at -20°C for 24 hours

Observations

FCS measurements were taken of the the 0.02% beads as well as solutions 2 and 5. According to our measurements, there were no spheres present in solutions 2 or 5. Because of this, we are going to prepare new sets of hydrogels (Part 4) in which we do not allow them to dry after filtration. We will instead immediately place them back into solution. We are hopeful that this will allow the hydrogels to stay separate instead of aggregating.