MEASUREMENT SOLUTIONS FOR BIO SCIENCES

Loligo® Systems develops research equipment for aquatic biology. Our products allow scientists all over the world to excel in the fields of swimming performance, respirometry, blood physiology and behavior analysis in a diversity of marine and freshwater organisms. We offer customized solutions as well as free scientific advice and support to anyone in need. Loligo® Systems is here to help.

Monitor, log and control oxygen and temperature in up to 24 independent tanks from a single computer with wireless Bluetooth communication. Fiber optic oxygen sensing technology in combination with a high-precision temperature and real-time compensation, offers unbeaten accuracy and stability of oxygen data as well as zero sensor maintenance. The user-friendly WitroxCTRL software makes it easy to calibrate, change measuring units, and set up automated protocols for changes in oxygen and temperature in steps or in a sinusoid manner (e.g. to mimic natural fluctuations like diurnal changes in oxygen saturation or temperature). The systems comes with all the necessary hardware and software, except for a Windows PC.

This system measures individual respiration in real-time in tiny organisms like Zebrafish embryos, Daphnia, fish/invert eggs or larvae, Drosophila etc., based on a 24-channel optical fluorescence oxygen reading device. Individual organism are placed inside gas-tight 80-1,700 µl glass chambers on a 24-well microplate footprint for real-time oxygen consumption rate measurements. Each chamber is fitted with a non-invasive and re-usable oxygen sensor spot that can be re-calibrated and sterilized for multiple trials. The microplate and reader is placed inside an incubator for temperature control, or use an optional flow-through water bath and refrigerated circulator. A soft silicone gasket with an optional line (e.g. parafilm) is placed on top of the microplate to seal the 24 chambers in one go. For high-throughput the system can be upgraded to include 10 readers/plates, i.e., for measuring respiration in 240 organisms simultaneously.