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Flow Cytometry: CCR7 Antibody (3D12) [NBP1-43332] - Analysis using the PE conjugate of NBP1-43332. Analysis of human peripheral blood cells using CCR7 PE conjugate. This can be used at less than or equal to 0.5 ug per ...read more

Applications/Dilutions

Dilutions

Flow Cytometry < = 0.5 ug /10^6 cells in 100 ul

Immunohistochemistry 1:10-1:500

Immunohistochemistry-Paraffin 1:10-1:500

Application Notes

This 3D12 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. It is recommended that the staining incubation time be increased to at least 45 minutes at 4°C for optimal staining.

Packaging, Storage & Formulations

Storage

Store at 4C. Do not freeze.

Buffer

PBS, pH 7.2

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Purity

Protein A or G purified

Alternate Names for CCR7 Antibody (3D12)

BLR2C-C chemokine receptor type 7

C-C CKR-7

CC-CKR-7

CCR7

CCR-7

CD197 antigen

CD197

CDw197CC chemokine receptor 7

chemokine (C-C motif) receptor 7

CMKBR7chemokine (C-C) receptor 7

EBI1EBV-induced G protein-coupled receptor 1

EBV-induced G-protein coupled receptor 1

Epstein-Barr virus induced gene 1

Epstein-Barr virus induced G-protein coupled receptor

Epstein-Barr virus-induced G-protein coupled receptor 1

EVI1

lymphocyte-specific G protein-coupled peptide receptor

MIP-3 beta receptor

Background

The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197. CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of nave from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DCs. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation. Expression of CCR7 on the cell surface can be down regulated upon ligand binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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