Features & Benefits:
• Simple one-step procedure; takes only 1-2 hours
• Fast and convenient
• Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-5 activity.

Description:
The Caspase-5 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspase-5 and related caspases that recognize the sequence WEHD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate WEHD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.