Development of Polymorphic Microsatellite Markers from AFLP Products in the Xinjiang Arctic Grayling (Thymallus arcticus grubei) and a Test of Cross-Species Amplification

1 Xinjiang University, College of Life Sciences and Technology, Urumqi 830046, China2 Linyi University, College of Life Sciences, Linyi 276000, China3 Liaoning Center for Disease Control and Prevention, Shenyang 110005, China
DOI :
10.4194/1303-2712-v19_4_08
Viewed :
598
-
Downloaded :
197
The population of Xinjiang arctic graylings (Thymallus arcticus grubei) has
dramatically declined in China. A total of 22 polymorphic microsatellite loci were
isolated and characterized from AFLP products in the Xinjiang arctic grayling. The
number of alleles (Na) per locus varied from 3 to 13. Observed (HO) and expected (HE)
heterozygosities ranged from 0.60 to 0.97, and from 0.58 to 0.89, respectively. These
new microsatellite markers are a helpful tool for genetic analyses, stock
management, and resource conservation efforts for T. arcticus grubei. According to
the total number of alleles, the total effective number of alleles, and the total
number of genotypes, the Fuhai population showed the highest diversity among all
the populations while the Fuyun sample was the lowest. Five additional fish species:
Red seam bream (Pagrus major), Lenok (Brachymystax lenok), Sea perch (Lateolabrax
japonicus), Taimen (Hucho taimen), and Amur grayling (Thymallus grubii) were
assessed for cross-species amplification. Three out of the five species showed at least
two polymorphic loci. In addition, six loci were found to be polymorphic in at least
one species.
Keywords :
Thymallus arcticus grubei, Microsatellite markers, Cross-species amplification