The antibody is reported to be strongly neutralizing and reactive using flow cytometry and Western blot analysis.

Emerging infectious disease research

Biosafety Level

1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation

Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E24 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E24 antibody is reported to bind to the lateral ridge of domain III in the envelope glycoprotein.

Product Format

frozen Each vial of VR-1612 contains approximately 100 µL of purified monoclonal antibody in PBS.

Storage Conditions

Store at -20°C or colder immediately upon arrival Repeated freeze-thaw cycles should be avoided

Comments

Antibody Class: IgG2aκ

Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E24 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E24 antibody is reported to bind to the lateral ridge of domain III in the envelope glycoprotein.

Functional activity is confirmed by reactivity in immunofluorescence assays using Vero cells (ATCC CCL-81) infected with WNV and by ELISA using WNV-infected cell lysates [WNV, Eg101 (Egypt 1951)]. The antibody is reported to be strongly neutralizing and reactive using flow cytometry and Western blot analysis.

Effect on Host

Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E24 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E24 antibody is reported to bind to the lateral ridge of domain III in the envelope glycoprotein.

Effect on Host

Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E24 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E24 antibody is reported to bind to the lateral ridge of domain III in the envelope glycoprotein.

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.