A reverse phase high performance liquid chromatographic method was developed for the determination of Mirabegron in bulk and Pharmaceutical dosage form. The separation was effected on a Waters ODS C18 column (150 mm x 3.9 mm;5µ) using a mobile phase mixture of buffer and acetonitrile in a ratio of 50:50 v/v at a flow rate of 1ml/min. The detection was made at 249 nm. The retention time of Mirabegron was found to be 2.502 min. Calibration curve was linear over the concentration range of 6.25-37.5 µg/ml of Mirabegron. The propose method was validated as per the ICH guidelines. The method was accurate, precise, specific and rapid found to be suitable for the quantitative analysis of the drug and dosage form.