Here is an interesting tidbit from a paper I was reading: "In addition to the expected tumor cell death, hyperthermia treatment has

Here is an interesting tidbit from a paper I was reading: "In addition to the expected tumor cell death, hyperthermia treatment has

also induced unexpected biological responses, such as tumor-specific immune responses as a result of heat-shock protein expression. These results suggest that hyperthermia is able to kill not only local tumors exposed to heat treatment, but also tumors at distant sites, including metastatic cancer cells." (Kobayashi)

also induced unexpected biological responses, such as tumor-specific immune responses as a result of heat-shock protein expression. These results suggest that hyperthermia is able to kill not only local tumors exposed to heat treatment, but also tumors at distant sites, including metastatic cancer cells." (Kobayashi)

Revision as of 04:35, 29 November 2012

This is a brainstorming page. You are very welcome to write any crazy / non-crazy / inventive / conventional / knowledgeable ideas or information you may have about our project.

Contents

What is Magnetic Hyperthermia?

Definition

How it works?

Here is an interesting tidbit from a paper I was reading: "In addition to the expected tumor cell death, hyperthermia treatment has
also induced unexpected biological responses, such as tumor-specific immune responses as a result of heat-shock protein expression. These results suggest that hyperthermia is able to kill not only local tumors exposed to heat treatment, but also tumors at distant sites, including metastatic cancer cells." (Kobayashi)

Current Research

Clinical trials in prostate cancer

Current Limitations(This information will help us shape and define the problem.)

(1) To achieve the necessary rise in temperature with minimal dose of MNP.

In other words, this means:

High specific loss power / specific absorption rate (SLP) of the MNP.

(2) Lack of knowledge about the metabolism, clearance, and toxicity of MNP.

Biomedical potentials of MNP

Could be used as early detection for the following using MRI:

Inflammation

Cancer

Diabetes

Atherosclerosis

Drug Delivery

Cellular labeling and tissue targeting

Purifying and separating cells and DNAs

Hyperthermia

Transfection by magnetic nanoparticles

Tissue repair

Magnetic resonance imaging (MRI)

Types of Relevant Viruses

1. Tobacco Mosaic Virus (TMV)

18nmx300nm, helical

Can withstand high temperatures up to 50C for 30mins (conventional hyperthermia involves heating up to 50C from an external source

Safe for human consumption

Mann group has active research on it

2130 molecules of coat protein

"*

2. M13 Bacteriophage

6.6nmx880nm, helical (Length is too long - pose an issue in targeting cells)

Lots of research done by the Belcher group, including attaching MNPs to M13 for imaging purposes

We are familiar with the system

3. Cowpea chlorotic mottle virus (CCMV)

26nm, icosahedral

4. Cowpea mosaic virus (CPMV)

27nm, icosahedral

5. Brome mosaic virus (BMV)

28nm, icosahedral

6. Turnip yellow mosaic virus (TYMV)

30nm, icosahedral

Current Work in Viral MNP Attachment

Attachment of MNPs to M13 phage for in vivo imaging of prostate cancer

What we propose to do

See flowchart sketch.

We will start with using ferritin (Fe3O4) as the MNP.

Future directions:

Experimenting with double layer MNP to increase response

Target other cancerous cells

Potential Issues

"The actual rotations of the nanoparticles are disordered because the microviscosity of the local environment in cancer cells is not constant, and effective elasticity depends on the binding conditions between nanoparticles and membranes."

but this is actually present because when treatment is done with individual MNPs, one side of the MNP is always bound to the targeted cell, so direction is never constant!