Description:
High copy number eukaryotic vector, pDC99 expresses a mutant luciferase gene under the control of the Simian virus 40Early Core (SV40) promoter. This vector encodes a mutant enzyme than catalyzes the production of long wavelength (red) light (Em:605) from D-luciferin,

Application:
This mutant luciferase is optimized for expression in mammalian cells, resulting in drastically improved light production, allowing detection of expression at extremely low levels. The mutant luciferase also has been shown to remain stable at 37°C over long time periods, a characteristic not seen in wild type luciferases. This vector is very useful for transfection of mammalian cells in culture and may also be used in other species. The mutant luciferase enzyme expression is enhanced by the Kozak's consensus sequence and SV40 late polyadenylylation signal. The pDC99 expression vector also contains the β-lactamase gene, which acts as a selection marker (100μg/mL ampicillin resistance) in E. coli host. In addition, the mutant luciferase gene can be excised using the XhoI/NotI sites to allow the insertion of other genes to be expressed under the same regulatory elements in mammalian cells. See also our CMV mutant luciferase vector system,

M1193 MarkerGeneTM Gaussia Luciferase Cellular Assay Kit, Utilizes a new luciferase which does not require ATP and can be expressed in mammalian cells using commercially available reporter plasmids, 1 kit MarkerGene 140

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BB24-AU-CE Homogenizer for 24 samples in microcentrifuge tubes. Large enclosure to help reduce noise and active cooling to maintain 4C interior temperature. Includes starter kit of beads, scoops and tubes. Up Next Advence 4850

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