Real Time Blues™- Hydrogen Peroxide/Peroxidase Assay

The versatility of real-time data collection with the convenience of an endpoint assay

Real-time BluesTM (RTB) - hydrogen peroxide/peroxidase-coupled assays are derived from a superior formulation of 3,3’,5,5’-tetramethylbenzidine (TMB). In the presence of peroxide and peroxidase the aromatic amine TMB contained is converted to produce a typically unstable blue chromophore intermediate with a peak absorbance at a wavelength of 650 nm. The RTB reagent was created to stabilize the blue chromophore for up to several days. Stable coloration enables assays to be performed in real-time or in endpoint experiments.

The RRB reagent is able to detect picomole quantities of hydrogen peroxide and picogram quantities of horseradish peroxidase (HRP) activity using a visible light spectrophotometer or by photographic analysis. The adaptable formula provides utility in a broad range of pH (3.5-9.0), temperature (4 – 60°C), and applications. RTB assays have been developed for applications in glycobiology, immunology, and cellular ROS (Reactive oxygen species) assays.

RTB - Hydrogen Peroxide/Peroxidase Detection Kit: This kit contains reagents for assaying at least 500 peroxide or peroxidase detection solutions in a 96-well microplate with a total sample volume of 100 μL per well.