Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

ICC/IF

Use at an assay dependent concentration. PubMed: 21887257

WB

Use a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 85 kDa. When looking at the phosphorylation of endogenous proteins, we recommend that IKKalpha first be immunoprecipitated.

Target

FunctionActs as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.

Tissue specificityWidely expressed.

Involvement in diseaseDefects in CHUK are the cause of cocoon syndrome (COCOS) [MIM:613630]; also known as fetal encasement syndrome. COCOS is a lethal syndrome characterized by multiple fetal malformations including defective face and seemingly absent limbs, which are bound to the trunk and encased under the skin.

Post-translationalmodificationsPhosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway.

Cellular localizationCytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus.

I can confirm that as ab105923IKK alpha antibodyhas not specifically been raised against a phosphorylated residue of the protein, it is therefore likely to detect both phosphorylated and non phosphorylated forms o...