Matrices listed below were spiked with certain level of FAS and the recovery rates were calculated by comparing the measured value to the expected amount of FAS in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

86-104

96

EDTA plasma(n=5)

85-105

92

heparin plasma(n=5)

85-101

92

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FAS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.