Specifications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Biological activityThe activity is determined by the dose dependent production of IL-8 from human PBMCs and is typically less than 25 ng/mL.

Applications

SDS-PAGE

HPLC

Endotoxin level< 0.100 Eu/µg

Purity> 95
% SDS-PAGE.Protein content and purity of ab119155 was determined by UV spectroscopy at 280 nm, RP-HPLC calibrated against a known standard and quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.

FormLyophilised

Additional notesIt is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.

Preparation and Storage

This product is an active protein and may elicit a biological response in vivo, handle with caution.

ReconstitutionLyophilized product is very stable at -20°C. Centrifuge vial before opening. When reconstituting, gently pipette and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute ab119155 with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

General Info

Alternative names

IL 31

IL-31

IL31

IL31_HUMAN

Interleukin 31

Interleukin-31

see all

FunctionActivates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity.

Recombinant mouse IL31 protein images

Other-IL31 protein (Mouse)(ab119155)

ab119155 used in Western Blot. Figure: 1 ug run under (+) reducing conditions and (-) non-reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Mouse IL-31 has a predicted MW of 15.7 kDa.

References for Recombinant mouse IL31 protein (ab119155)

ab119155
has not yet been referenced specifically in any publications.

Publishing research using ab119155? Please let us know so that we can cite the reference in this datasheet.