PCR conditions

Agarose gel electrophoresis

Perform an agarose gel electrophoresis of 20 μL of each diluted reaction using a 0.8% E-Gel®.

Also electrophorese 1 μg of 2-log DNA ladder to verify the length of each PCR product.

Image gel with 302 nm transilluminating ultraviolet light using an ethidium bromide camera filter and an exposure time of 614 milliseconds.

BioCoder version

Following is the Engineering BioBrick vectors from BioBrick parts/Colony PCR protocol in BioCoder, a high-level programming language for expressing biology protocols. What you see here is the auto-generated text ouput of the protocol that was coded up in BioCoder (see Source code). More information about BioCoder can be found on my home page. Feel free to mail me your comments/ suggestions.Vaishnavi