GeneRead Size Selection Kit

For quick and reliable removal of DNA fragments <150 bp for library preparation in NGS applications

Precise size selection of DNA fragments

Fast procedure, based on QIAGEN’s proven silica-column technology

An easy-to-follow protocol, automatable on the QIAcube

The GeneRead Size Selection Kit enables precise size selection and purification of DNA fragments using a fast and convenient spin-column-based procedure. The kit contains MinElute columns and an optimized binding buffer for the selection of DNA fragments larger than 150 bp. Procedures can be automated on the QIAcube Connect.

The GeneRead Size Selection Kit combines the convenience of spin-column technology with the selective properties of a uniquely designed binding buffer. MinElute spin columns provided with the kit ensure high concentrations of purified DNA for subsequent reactions. The special buffer provided with the kit is optimized for efficient removal of small DNA fragments such as adapters and adapter dimers. Larger DNA fragments adsorb to the silica membrane in the presence of optimized concentrations of salt, while contaminants and smaller fragments pass through the column. This leads to a cut-off limit (defined as the length of the shortest fragment that can be visualized by electropherogram) of 150 bp. Impurities such as enzymes from previous reactions are efficiently washed away, and pure and size-selected DNA is eluted. For optimal adapter monomer and dimer removal, together with high DNA recovery, two subsequent purification steps are performed for size selection during library preparations for next-generation sequencing.

Procedure

The GeneRead Size Selection Kit uses a convenient, spin-column-based procedure to ensure precise size selection of the DNA library, while effectively removing adapter dimers or monomers. Automatable on the QIAcube, the easy-to-use protocol saves time by eliminating tedious handling procedures, and ensures there is no risk of carryover of potentially inhibitory ethanol or beads (see figure Precise size selection).

Applications

The GeneRead Size Selection Kit enables quick and reliable removal of DNA fragments shorter than 150 bp for subsequent use in library preparation in NGS.