Homology

Species predicted to react based on 100% sequence homology:
Chicken,
Bovine,

Specificity / Sensitivity

Phospho-MARCKS (Ser167/170) (D13E4) XP® Rabbit mAb recognizes endogenous levels of MARCKS protein only when phosphorylated at Ser167 and Ser170. This antibody may also detect MARCKS mono-phosphorylated at Ser167.

Background

Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a major PKC substrate expressed in many cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic, and mitogenesis (1). PKC phosphorylates Ser159, 163, 167, and 170 of MARCKS in response to growth factors and oxidative stress. Phosphorylation at these sites regulates the calcium/calmodulin binding and filamentous (F)-actin cross-linking activities of MARCKS (2-4). Phosphorylation by PKC also results in translocation of MARCKS from the plasma membrane to the cytoplasm (5).