HPLC, column and thin layer chromatography guided studies led to the isolation of seven different
compounds in methanolic extracts of Podophyllum hexandrum. The isolated compounds were analyzed using
LC-MS and High Performance Liquid Chromatography (HPLC) studies interfaced to mass spectroscopy.
Isolated compounds were used successfully as chemical markers for the comparison of the twelve different
accessions of Podophyllum. We have also shown that the variation of chemical composition in P. hexandrum
agree well with their botanical phylogeny as revealed by genetic phylogeny. HPLC analysis also revealed
development of valuable chemotypes containing higher concentration of isolated marker compounds.