Antigene oligodeoxynucleotide

In article <9508260149.AA13355 at phy>, kappei at basic2.kpu-m.ac.jp (Kappei
KOBAYASHI) wrote:
> Dear netters,
> I'm considering the design of antigene oligodeoxynucleotide (ODN)
> to control stress gene expression. I intend to synthesize 13-mer ODN
> consisted of thymine and 5-methyl-cytosin and 5'-linked acrydine,
> which is expected to form triplex on stress 70 promoter.
>> I hope someone to tell me whether or not
> 1)phosphorothioate modification of the ODN is needed?
This will stabilize the oligo towards nuclease digestion, but unfortunately
will probably abolish any triplex formation...
> 2)a single mismatch at 3' terminus reduce the stability of expected
> triplex?
Not very much. Do you wish to descriminate a single base mismatch at the
3' end?
> 3)there are some other strategies (or modification) of ODN to improve it
> in the ability to shutt off the gene expression?
I am currently working on a new backbone analog which should serve very
well in your application. If you are interested in a formal collaboration,
please e-mail me. (I work for a private company and we need to have
written agreements and such...)
David H. Lloyd, Ph.D.
<dhl at best.com>
Lynx Therapeutics, Inc.
(
> Other recent info on antigene ODN would be appreciated.
>> Thanks in advance
>> Kappei Kobayashi (kappei at basic2.kpu-m.ac.jp)