Phencyclidine, also known as PCP or Angel Dust, is a hallucinogen
that was first marketed as a surgical anesthetic in the 1950’s. It
was removed from the market because patients receiving it became
delirious and experienced hallucinations. Phencyclidine is used in
powder, capsule, and tablet form. The powder is either snorted or
smoked after mixing it with marijuana or vegetable matter.
Phencyclidine is most commonly administered by inhalation but can
be used intravenously, intranasally, and orally. After low doses,
the user thinks and acts swiftly and experiences mood swings from
euphoria to depression. Self-injurious behavior is one of the
devastating effects of Phencyclidine.

The One Step PCP (Pencyclidine) Test Strip is an immunoassay based
on the principle of competitive binding. Drugs that may be present
in the urine specimen compete against the drug conjugate for
binding sites on the antibody. During testing, a urine specimen
migrates upward by capillary action. Phencyclidine, if present in
the urine specimen below 25 ng/mL, will not saturate the binding
sites of the antibody in the test strip. The Phencyclidine
conjugate will be captured by antibody and a visible colored line
will show up in the test line region. The colored line will not
form in the test line region if the Phencyclidine level exceeds 25
ng/mL because it will saturate all the binding sites of
anti-Phencyclidine antibodies.

Clinical Test

Test

Drug-free

Low Negative (Less than half the cutoff concentration)

Near Cutoff Negative (Between 50% below the cutoff and the cutoff
concentration)

Near Cutoff Positive (Between the cutoff and 50% above the cutoff
concentration)

High Positive (greater than 50% above the cutoff concentration)

Operator A

Positive

0

0

0

13

24

Negative

10

15

15

3

0

Operator B

Positive

0

0

0

13

24

Negative

10

15

15

3

0

Operator C

Positive

0

0

0

13

24

Negative

10

15

15

3

0

Operation

NEGATIVE:* Two lines appear. One red line should be in the control region
(C), and another apparent red or pink line should be in the test
region (T). This negative result indicates that the Phencyclidine
concentration is below the detectable level (25 ng/mL). *NOTE: The shade of red in the test line region (T) will vary, but it
should be considered negative whenever there is even a faint pink
line.

POSITIVE: One red line appears in the control region (C). No line appears
in the test region (T). This positive result indicates that the
Phencyclidine concentration exceeds the detectable level (25
ng/mL).

INVALID: Control line fails to appear. Insufficient specimen volume or
incorrect procedural techniques are the most likely reasons for
control line failure. Review the procedure and repeat the test with
a new test strip. If the problem persists, discontinue using the
test kit immediately and contact your local distributor.