The crispr.kitchen connects biohackers and makers who develop their own technologies with industry and academics to exchange about the future of citizen made technology in Germany. By discussing latest prototypes and in interactive games, we will explore the potentials of DIYscience in the innovation system and society.

So now that JoVE (the Journal of Visualized Experiments) is no longer Open Access - does anyone know of a good way to download their videos locally? The JoVE videos are wonderful, especially for DIYbio practitioners who may have no other way of seeing how these things are done in a real lab.

Someone posted a method a couple of years ago to circumvent their security-through-obscurity by sifting through the html source code, but that approach doesn't seem to be working anymore. I wouldn't be surprised if some version of this still holds though:

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.

BI Developer

Mountain View, CA

6+ Months

Comments / Special Conditions PRIMARY SKILL: Advanced SQL/Tableau

Advanced SQL Skills(ability to not only modify a script, but be able to adjust and write your own as necessary, knowledge of temp tables, joins, performance tuning etc).Advanced MS ExcelSkills (beyond vlookups and Pivot Tables. Experience in: Index/match, sumproduct, offset etc).Experience in design, development, and implementation of Business Intelligence applications including Business Analysis and ETL.Experience developing reports inTableau (2-4 years experience is a MUST)

I think lots of international students go to Germany and Austria, who have hardly any study fees at all. But then, if you want a job or change the world, you'll have to go back to the US, the only country where your genetically enhanced plants/bacteria will ever be allowed outside labs. And still it takes a lot of time there to get approval

On Sunday, May 28, 2017 at 8:36:45 PM UTC+2, Ayush Mahajan wrote:

Which country would be affordable to study Bioengineering ?

On Thursday, May 25, 2017 at 2:58:41 PM UTC+5:30, John Griessen wrote:

On 05/25/2017 04:03 AM, Mega [Andreas Stuermer] wrote: > Tell the voice in your head to shut up, a lot of people that are less intelligent than you have done it already ;) >

Repeating an offlist email: On 05/24/2017 12:43 PM, Ayush Mahajan wrote: > thanks, I'm thinking to get Biotech as minor and CSE as majors would > this help me overcome my fear?

Only you know your fears very well. Others have their own fears.

Sometimes fear can be F.E.A.R. ==> False Evidence Appearing Real.

Finding people doing what you are interested in and asking them how they do it will dispel any groundless fears, or show you that they do some things that you are not good at yet, or are avoiding, or maybe even involve fears you have.

Biomedical Engineering channels one into working for companies that get their designs qualified for medical use, which can be laborious and costly, especially in the US.

Studying biotech, while also getting a degree in CS will help you find work outside the medical field. But both are fairly broad, not guaranteeing any particular kind of work...

Always be thinking of what work is going to be like, and will you like that, and ask people that are getting experience again and again what that is like.

also think a lot about what the world needs that you can deliver, or you and some others can deliver...

If you don't enjoy following others orders like me, that means learning how to run businesses, and how to get some dirt/land/buildings owned or long term leased to operate on, so you can do your own thing manufacturing-wise.

People have used DIY gene guns, which doesn't require agrobacterium which is considered a plant pest. And it's really just pressurized gass shooting DNA-coated metal-nanoparticles into plant cells. Although it's unlikely that that disarmed lab strain remains viable in the wild (they lose their advantage of forcing plant cells to feed them) it may be smart to circumvent any regulatory issues.

Depending on the strain and plasmids you work with, some antibiotics might work. Kanamycin or streptomycin. Tetracyclin is not recommended as they can develop natural resistance very easily, for what I heard.

In practiuce you have to subculture the regenerated plantlets several times with antibiotics to make reasonably sure all the agrobacterium will be gone.

Some protocols grow them in the dark, so I am confused. If so, what lights/cycle do you recommend?

By the time you generate calli, you will have enough to run experiments. Some in the light, some in the dark. Try 12/12 for those in the light.

My purpose for for growing calluses (calli?) would be two-fold: somatic embryogenesis, and transformation with Agrobacter in Crassula.

2. Can I use parafilm to seal the callus-growing petri dishes? What about gas exchange?

To seal the dishes around the edges? Yes. Parafilm is not air-tight. Carbon dioxide and ethylene will diffuse in and out; ditto with water and oxygen. Parafilm tends to embrittle and get useless after a period of time. You may want to try other plastic wrap like Saran Wrap or other plastic films, or medical paper tape.

3. Would the PPM in the callus media kill Agrobacter?

Never count on PPM being -cidal. You'll have to run tests on media without selective agents to demonstrate the agro is either gone, or hiding so well that you can't easily find it.

On 05/29/2017 11:55 AM, Sebastian Cocioba wrote:
> Doing whole genome sequencing on a plant. Used nanopore reads and currently assembling plastid genomes and then will use Amazon
> AWS to do the nuclear assembly. Largest computational project Ive done thus far. Bioinformatics has become the cornerstone of my
> research effects in my home lab.

Sounds close to GMO time. Will you be trying DNA that has some errors from reads and assembly, and do you have any redundant
DNA in there to offset errors?

Doing whole genome sequencing on a plant. Used nanopore reads and currently assembling plastid genomes and then will use Amazon AWS to do the nuclear assembly. Largest computational project Ive done thus far. Bioinformatics has become the cornerstone of my research effects in my home lab.

The most sophisticated is using standard assemble to reference tools within Galaxy for a fungal genome that I had MWG Operon sequence for me on an Illumina HiSeq machine. See about halfway down this page for a brief description.http://roningenetics.org/Sequencing.html

I've been asked to be on a National Academy of Sciences panel this Thursday about synbio and DIYbio (together with Drew Endy, and Tom Burkett from BUGSS), and I'm supposed to talk about how computational tools are enhancing ease of use in DIY labs. Figured I'd do a quick poll of the rest of the community...

On Thursday, May 25, 2017 at 2:58:41 PM UTC+5:30, John Griessen wrote:

On 05/25/2017 04:03 AM, Mega [Andreas Stuermer] wrote: > Tell the voice in your head to shut up, a lot of people that are less intelligent than you have done it already ;) >

Repeating an offlist email: On 05/24/2017 12:43 PM, Ayush Mahajan wrote: > thanks, I'm thinking to get Biotech as minor and CSE as majors would > this help me overcome my fear?

Only you know your fears very well. Others have their own fears.

Sometimes fear can be F.E.A.R. ==> False Evidence Appearing Real.

Finding people doing what you are interested in and asking them how they do it will dispel any groundless fears, or show you that they do some things that you are not good at yet, or are avoiding, or maybe even involve fears you have.

Biomedical Engineering channels one into working for companies that get their designs qualified for medical use, which can be laborious and costly, especially in the US.

Studying biotech, while also getting a degree in CS will help you find work outside the medical field. But both are fairly broad, not guaranteeing any particular kind of work...

Always be thinking of what work is going to be like, and will you like that, and ask people that are getting experience again and again what that is like.

also think a lot about what the world needs that you can deliver, or you and some others can deliver...

If you don't enjoy following others orders like me, that means learning how to run businesses, and how to get some dirt/land/buildings owned or long term leased to operate on, so you can do your own thing manufacturing-wise.

Tell the voice in your head to shut up, a lot of people that are less intelligent than you have done it already ;)

It may seem scary at the beginning, but there's a system behind it. And once you see the matrix behind the things, everything makes sense and you know what you need to look up when you get there.

There will be lab exercises, it's not just theory?

Me personally, I have had occasions where I wouldn't understand something in detail in a lecture, specifically in no-biotech subjects. But then you either do it in the lab - or just google around connected areas and applications and downstream processes and everything makes sense and the theory is stuck in your head. I never liked learning a row of words in the correct order, I would need to see what the purpose of "Process X" is and how I can use it, to incentivize my brain to store the info

On Wednesday, May 24, 2017 at 12:13:15 AM UTC+2, Ayush Mahajan wrote:

Yes, I already know how to code, reverse engineer and rest of the stuff which a computer engineer can do, the thing is I want to take BioTechnology for my undergrad but I'm scared because I've never been good with the theory part.I'm interested in coding genome, hacking the wetware and everything Biohacker could do but every time I think about Biotech a voice whispers me in my head saying "Would you be able to do it ?"

On Sunday, May 21, 2017 at 1:32:38 AM UTC+5:30, Ayush Mahajan wrote:

I've to choose a course now but I'm way too confused between BME and CSE? I'm excellent in coding but I'm interested to learn about this field. My only question is how difficult is it going to be for newbie to start studying about BME and is it a good idea?

On Tue, May 23, 2017 at 2:37 PM, Ayush Mahajan <blue...@gmail.com> wrote: > Yes, I already know how to code, reverse engineer and rest of the stuff > which a computer engineer can do, the thing is I want to take BioTechnology > for my undergrad but I'm scared because I've never been good with the theory > part.I'm interested in coding genome, hacking the wetware and everything > Biohacker could do but every time I think about Biotech a voice whispers me > in my head saying "Would you be able to do it ?"

Sounds like I had a similar experience with being good at coding when I chose a school, but wanting more. The cooler stuff. I've not heard of computer related protests as much as I have about biotech. Light a candle and shed some light on the darkness.

Biotech /school/ is mostly just fancy cooking, and learning the theory is too much to remember for the average student. The 'computer' processes of life are lots controlled chemical reactions. You don't need to be so smart as a chemistry major, or a chemical engineer... biotech has less math requirements than they do. Biotech also has less math requirements than Computer Science.

Humans engineered electronic computers taught in Computer Science. It has been around (in text/print/books) for a long time. Biotech is about reverse-engineering innumerable systems we didn't engineer. It is like CS for the stuff we didn't engineer.

We are reverse engineering ourselves and all the other species. We are reverse engineering chemistry, and thus physics.

I know a little about Indian school systems, and if that's where you're heading towards, you won't have much room to explore side-studies in your school. The academic programs are very structured and have strict/tight timelines.

I'd recommend studying biotech, simply because the hands-on and theory is a lot wider ranging in topic than CS will give you. Also, you can learn CS at pretty much any hackerspace, free online courses, reading self-teach books, etc... It is more like 'common knowledge' than biotech and chemistry/physics. For CS you need electricity and some relatively common and cheap electronics gizmos. Biotech/lab-science involves a lot of heavy (mass-wise) stuff, has lots of steps that take a long time and require a special cook and special kitchen. You can compile code anywhere your laptop has charge... not as easy to say the same about a really complex Biotech project.

You can do it. There were many underperforming Biotech students who received better grades than I did. It's all in how you play the game. You won't be an underperformer. I know because you're already self-motivated enough to come post on this online forum. Most students lack self-motivation. Keep your motivation. It is what keeps you going in the face of being penniless and without a job. It is a strong driver of innovation.

Some protocols grow them in the dark, so I am confused. If so, what lights/cycle do you recommend?

By the time you generate calli, you will have enough to run experiments. Some in the light, some in the dark. Try 12/12 for those in the light.

My purpose for for growing calluses (calli?) would be two-fold: somatic embryogenesis, and transformation with Agrobacter in Crassula.

2. Can I use parafilm to seal the callus-growing petri dishes? What about gas exchange?

To seal the dishes around the edges? Yes. Parafilm is not air-tight. Carbon dioxide and ethylene will diffuse in and out; ditto with water and oxygen. Parafilm tends to embrittle and get useless after a period of time. You may want to try other plastic wrap like Saran Wrap or other plastic films, or medical paper tape.

3. Would the PPM in the callus media kill Agrobacter?

Never count on PPM being -cidal. You'll have to run tests on media without selective agents to demonstrate the agro is either gone, or hiding so well that you can't easily find it.

After three years of court proceedings in Colombia, a judge in Bogotá acquitted a graduate student yesterday (May 24) of charges he violated copyright law by sharing another researcher’s masters thesis online for a study group. If he had been found guilty, 29-year-old Diego Gomez could have faced years in prison.

“This case must spark a serious debate over the necessity of Open Access,” Carolina Botero, director of an organization called Fundación Karisma, which has been helping Gomez with his legal case, said in a press release sent to The Scientist. “Today we celebrate that justice was made in an absurd case that could have set a bad precedent for access to knowledge in Colombia.”

Gomez was studying biology at the University of Quindio in Armenia in central Colombia several years ago when he uploaded another student’s thesis to Scribd, a digital library and e-book and audiobook subscription service. Unlike in the United States, copyright violations in Colombia are a criminal act, and the thesis author pressed charges.

“When uploading the thesis I never thought I was violating any law,” Gomez told The Scientist in 2014. “This type of literature is not of commercial interest, so I never thought I could do any damage to the author. On the contrary, I thought that I was giving him benefits on sharing his work.”

Colombia’s copyright law was born out of an agreement with the United States, intended to bolster trade. “Diego’s story also serves as a cautionary tale of what can happen when copyright law is broadened through international agreements,” the Electronic Frontier Foundation, an advocacy group for free speech and Internet access, posted on its website today. “But as is often the case when trade agreements are used to expand copyright law, the agreement only exported the U.S.’ extreme criminal penalties; it didn’t export our broad fair use provisions.”

After three years of court proceedings in Colombia, a judge in Bogotá acquitted a graduate student yesterday (May 24) of charges he violated copyright law by sharing another researcher's masters thesis online for a study group. If he had been found guilty, 29-year-old Diego Gomez could have faced years in prison.

"This case must spark a serious debate over the necessity of Open Access," Carolina Botero, director of an organization called Fundación Karisma, which has been helping Gomez with his legal case, said in a press release sent to The Scientist. "Today we celebrate that justice was made in an absurd case that could have set a bad precedent for access to knowledge in Colombia."

Gomez was studying biology at the University of Quindio in Armenia in central Colombia several years ago when he uploaded another student's thesis to Scribd, a digital library and e-book and audiobook subscription service. Unlike in the United States, copyright violations in Colombia are a criminal act, and the thesis author pressed charges.

"When uploading the thesis I never thought I was violating any law," Gomez told The Scientist in 2014. "This type of literature is not of commercial interest, so I never thought I could do any damage to the author. On the contrary, I thought that I was giving him benefits on sharing his work."

Colombia's copyright law was born out of an agreement with the United States, intended to bolster trade. "Diego's story also serves as a cautionary tale of what can happen when copyright law is broadened through international agreements," the Electronic Frontier Foundation, an advocacy group for free speech and Internet access, posted on its website today. "But as is often the case when trade agreements are used to expand copyright law, the agreement only exported the U.S.' extreme criminal penalties; it didn't export our broad fair use provisions."

I am gearing up towards a plant DIY operation, and I was hoping you could answer a couple of questions that I am having a hard time finding on the internet.

1. Do calluses need light to grow? Some protocols grow them in the dark, so I am confused. If so, what lights/cycle do you recommend? My purpose for for growing calluses (calli?) would be two-fold: somatic embryogenesis, and transformation with Agrobacter in Crassula.

2. Can I use parafilm to seal the callus-growing petri dishes? What about gas exchange?

We are looking for individuals who have the ability to write efficientJava code extracting, transforming and loading (ETL) data into Splunk.You must have experience with extracting or exporting data from third party systems using an API or programmatically, and then transforming or formatting the data before loading or pushing the extracted data into a third party application such as Splunk. Excellent communication skills will be required to be effective in this role.