Overview

Replacement Information

Description

Catalogue Number

FCCH100108

Trade Name

FlowCellect

Description

FlowCellect Annexin Red Kit

Overview

Millipore’s FlowCellect Annexin Red Kit provides a rapid and convenient assay to monitor early and late apotosis. The kit includes recombinant Annexin V conjugated to a red sensitive dye, CF647, to provide maximum sensitivity and 7-AAD, a cell impermeant dye which is an indicator of cell membrane structural integrity along with an assay buffer. 7-AAD is excluded from live, healthy cells and early apoptotic cells but stains late stage apoptotic and dead cells with red fluorescence by binding to nucleic acids in the cell. After staining cells with Flowcellect Annexin Red Kit, three populations of cells can be identified in this assay:

The kit permits users to pair the provided reagents with other reagents that fluoresce in the green and yellow channels of the flow cytometer. Sufficient reagents are provided for 100 tests. The kit includes all optimized fluorescently labeled reagents and buffers necessary for cell preparation and analysis.

Alternate Names

FlowCellect

References

Product Information

Components

Annexin VCF647

7AAD

10X Assay Buffer

User Manual

Detection method

Fluorescent

Instrument compatibility

EasyCyte 8HT

Applications

Application

This FlowCellect Annexin Red Kit for flow cytometry provides a rapid & convenient assay to monitor early & late apotosis.

Key Applications

Flow Cytometry

Biological Information

Species Reactivity

Human

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Ubiquitin-like containing PHD and RING finger 1 (UHRF1) contributes to silencing of tumor suppressor genes by recruiting DNA methyltransferase 1 (DNMT1) to their hemi-methylated promoters. Conversely, demethylation of these promoters has been ascribed to the natural anti-cancer drug, epigallocatechin-3-gallate (EGCG). The aim of the present study was to investigate whether the UHRF1/DNMT1 pair is an important target of EGCG action. Here, we show that EGCG down-regulates UHRF1 and DNMT1 expression in Jurkat cells, with subsequent up-regulation of p73 and p16(INK4A) genes. The down-regulation of UHRF1 is dependent upon the generation of reactive oxygen species by EGCG. Up-regulation of p16(INK4A) is strongly correlated with decreased promoter binding by UHRF1. UHRF1 over-expression counteracted EGCG-induced G1-arrested cells, apoptosis, and up-regulation of p16(INK4A) and p73. Mutants of the Set and Ring Associated (SRA) domain of UHRF1 were unable to down-regulate p16(INK4A) and p73, either in the presence or absence of EGCG. Our results show that down-regulation of UHRF1 is upstream to many cellular events, including G1 cell arrest, up-regulation of tumor suppressor genes and apoptosis.