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Recombinant Human IFNB1

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Product

Source

Size

Cat. No.

Price

Recombinant Human IFNB1

CHO cells

20 µg

Z101485

$150.00

1.0 mg

Z101489

$3,500.00

Cat. No.

Product Name

Qty.

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IFN-beta is produced mainly by fibroblasts and some epithelial cell types. The synthesis of IFN-beta can be induced by common inducers of interferons including viruses, double-stranded RNA, and micro-organisms. It is induced also by some cytokines such as TNF and IL-1. IFN-beta binds to the same receptor as IFN-alpha. IFN-beta is involved in the regulation of unspecific humoral immune responses and immune responses against viral infections. IFN-beta increases the expression of HLA class 1 antigens and blocks the expression of HLA class 2 antigens stimulated by IFN-gamma. IFN-beta stimulates the activity of natural killer cells and hence also antibody-dependent cytotoxicity. IFN-beta shows antiproliferative activity against a number of cell lines established from solid tumors.

Gene Symbol

IFNB1

Gene ID

3456

Accession No.

P01574

Source

CHO cells

Appearance

Lyophilized Powder

Molecular Weight

19.0 kDa

Endotoxin Level

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Purity

>95% as determined by SDS-PAGE

Bioactivity

The activity was determined by the viral resistance assay of Human WISH cells was found to be in the range of 3.0 x 10^8 IU/mg.

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Storage

The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

1. For estimating the endotoxin levels; we use the LAL (Limulus Amebocyte Lysate) method: The lysate from horseshoe crab amebocytes clots in the presence of very low endotoxin. This reaction is the basis of the Limulus amebocyte lysate (LAL) assay which was approved by the FDA in 1970.

· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.

With regard to the BSA levels in some Growth Factors and Cytokines, can you please provide an explanation as to why they are so high?

The amount of BSA, as part of the formulation of a protein, can vary considerably depending on how much BSA was deemed optimum/necessary for protein stability in combination with /in-lieu of - other possible additives. The aforementioned formulations are somewhat analogous to the “carrier” versions of many formulations from “R and D systems” that have as high as 50 µg of BSA per µg of the recombinant protein product. If, needed or desired, abm scientists can substitute BSA for other stabilizing additives for most formulations.

Yes, all of abm's growth factors made in Escherichia coli using recombinant technology contain no human derived-products or other recombinant proteins. In the rare cases of BSA presence, this will be mentioned in the product's formulation.