The
purpose of this study is to measure and establish the metabolite change when
the Muscle Derived Stem Cells (MDSCs) were differentiated into adipocyte
using the 1H MR Spectroscopy. The experiments are classified four groups:
Group1-Adipogenic Media, Group2- Papain digested fibrin gel, Group3-Papain
digested MDSCs, Group4-Papain digested adipogenic MDSCs. The spectrum from
each group has been acquired by utilizing vertical-bore 14.1T NMR/MRI with
PRESS pulse sequence. Compare to spectrums of each group, we analyzed
metabolite peaks newly formed during the differentiation of the MDSCs. In the
results, we can observe that 1H MR spectral peak intensity increases at
0.89/1.24/1.9/2.48/3.0 ~3.1ppm after 14 days of differentiation from MDSCs
into adipocyte. In this study, therefore we could observe the metabolite
change along with MDSCs differentiation and found the potential possibilities
of MRS to evaluate the differentiations of stem cell.