Abstract

Antrodia cinnamomea is peculiar to Taiwan. It only grows on one host and is highly
valued as an important component of several traditional Chinese medicines. In this
study, the different protein expression profiles of artificially cultivated vegetative
mycelium and wild-type basidiomatal fruiting bodies were compared and unique protein
spots from wild-type basidiomatal fruiting body were investigated using 2D polyacrylamide
gel electrophoresis and LC-MS/MS protein identification. Most of the wild-type proteins
not seen in the artificially cultivated mycelium were associated to function in metabolism,
cell stress, ROS scavenging, and cell growth. Several proteins from wild-type basidiomes,
such as catalase, aryl-alcohol dehydrogenase, S-adenosyl-L-homocysteine hydrolase,
intradiol dioxygenase, haloacid dyhydrogenase, alpha- and beta-form tubulin, prohibitin,
septin, chaperone, and HSP90 ATPase, showed higher expression than those from artificially
cultured mycelium at the mRNA level.