HSP90β (C-Terminal Domain) TR-FRET Kit

Catalog #: 50262

Size: 384 Reactions

$1,040

Description:

The HSP90β (C-Terminal) TR-FRET assay kit is designed to measure the inhibition of HSP90α
binding to its protein target PPID (also known as Cyclophilin D or Cyp40) in a homogeneous,
384 reaction format. This assay requires no time-consuming washing steps, making it especially
suitable for high throughput screening applications. The assay procedure is straightforward and
simple. A sample containing terbium-labeled donor, dye-labeled acceptor, HSP90β, substrate,
and an inhibitor is incubated for 2 hours. The protein-protein interaction is then assayed by
measuring the TR-FRET between the PPID and HSP90β using a fluorescence reader.

UniProt

P08238

Synonym(s):

HSP90b, Heat Shock Protein 90b, HSP90beta, HSP90β, HTRF

Supplied As:

Comes in a homogeneous, 384 reaction format

Format:

Instructions for use:

See assay kit data sheet for detailed protocol

Storage / Stability:

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):

Great for screening small molecular inhibitors for drug discovery and HTS applications.