This study was conducted in Diyala province for the period from 1/10/2014 to 1/04/2015 as it collected 50 samples using sterile cotton swabs from people who suffer from gum inflammation disease in specialized centers of the teeth in the city of Baquba, and then the samples were transferred to microbiology laboratory/ Baquba reaching Hospital since been diagnosed with bacterial isolates These were: Escherichia coli, Streptococcus pyogenes. The study also included qualitative detection of the active compounds in plants Hibiscus sabdariffa, as well as the study of the influence of alcoholic extract, hot and cold water extract of the plant Hibiscus sabdariffa and three concentrations of 50, 100 and 200 mg ml -1. Results showed that the qualitative detection of plant Hibiscus sabdariffa contain many active compounds which alkaloids, saponins, tannins, filavonat, Alkleikosadat, volatile oils and resins. Hibiscus sabdariffa extracts showed inhibitory effective against bacterial isolates under study, since these isolates were sensitive primarily to extract alcohol and aqueous extract hot water extract followed by cold, and the effectiveness inhibitory of plant extract increased by increasing its focus, as it gave focus 200 mg.ml -1. Higher effective inhibitory toward bacterial species.