AbstractThe present study was conducted to measure various parameters of head morphometric of the Malayan gaur bulls (Bos gaurus hubbacki) spermatozoa through image analysis. Spermatozoa head morphometric was quantified in terms of the following morphological features: length, width, head shape (width: length) and head area. A total of 200 spermatozoa with intact acrosomes were selected, stained with eosin-nigrosin, and assessed with bright field microscopy at 1000x magnification. Mean head length, mean head width, head shape and mean head area were 9.06 ± 0.34 μm, 4.70 ± 0.28 μm, 0.51 – 0.53 and 42.60 μm2, respectively. As a conclusion, morphologically the Malayan gaur spermatozoa in this study show the normal features characteristics with the regular oval head morphometry.