Application Information: TCF4 antibody [0.T.149]

For ICC: Use at an assay dependent dilution. For IHC-P: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at a concentration of 0.5-2 μg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 71 kDa). Optimal dilutions/concentrations should be determined by the researcher.

Positive Controls

SW620 whole cell lysate

Form Supplied

Liquid

Concentration

1
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Protein G purified

Specifications: TCF4 antibody [0.T.149]

Full Name

transcription factor 4

Product Description

Mouse monoclonal [0.T.149] to TCF4

Background

T cell transcription factor-4 (TCF-4) is a member of the TCF-LEF family of transcription factors. TCF-4 interacts with b-Catenin in the Wnt signaling pathway to activate transcription of Wnt target genes. TCF-4 plays important roles in embryogenesis and oncogenesis. High levels of TCF-4 expression was found in developing CNS and limb buds. (In adults, TCF-4 is widely expressed.) Brain is the major site of expression, however, TCF-4 mRNA is also found in high levels in liver, lower in heart, lungs, kidneys, and testis, and lowest in spleen and muscle. In human TCF-4, four alternative splice sites are observed which correspond to the synthesis of many hTCF-4 isoforms with short, medium, or long-C-terminal ends (ADDINENRfu). BLAST searches showed these isoforms are very similar to each other, except those that are close to the C-terminus, however, the calculated molecular weights ranged from 52kD to 68kD. For mouse TCF-4, BLAST searches showed different isoforms with calculated molecular weights that range from 29kD to 51kD.