Activin-B is a TGF-beta family member that exhibits a wide range of biological activities including regulation of embryogenesis, osteogenesis, hematopoiesis, reproductive physiology and hormone secretion from the hypothalamic, pituitary and gonadal glands. Activin B, like certain other members of the TGF-β family, signals through the ActRII receptor (Activin Receptor type II). Human Activin B is a 26.0 kDa disulfide-linked homodimer consisting of two βB chains, each containing 115 amino acid residues.

The lyophilized protein is stable for a few weeks at room temperature,
but best stored at -800C. Reconstituted Human Activin-B should be stored in
working aliquots at -800C.

Purity :

Greater than 95% by SDS-PAGE and HPLC analysis.
Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Reconstitution :

We recommend a quick spin followed by reconstitution in
water to a concentration of 50 μg/ml (e.g., 5 μg/100μl) will yield a solution of
10 mM Citric Acid, pH 3.0. This solution can then be stored at room temperature or
at 40C for 1 month. It is recommended that further dilutions be made in PBS
containing 2 mg/ml albumin and stored at -800C.

Biological Activity :

The ED50 was determined by ability to inhibit
the murine MPC-11 cells. The expected ED50 is 0.5 - 2.0 ng/ml.