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Abstract

Introduction: Inflammation and coagulation are deeply interconnected phenomena, a mechanism amplified by Angiotensin(AT)II that upregulates Tissue Factor (TF), the major regulator of haemostasis and thrombosis through AT-1 receptor (R). Proinflammatory stimuli such as high glucose (HG) and lipopolysaccharide (LPS) induce TF expression by activating NFkB-mediated gene transcription and stimulating the intracellular synthesis of RAS components. Whether miRNAs participate to that process by post-transcriptional modulation of TF expression is unclear.

Hypothesis: We studied the role of miR-19a, miR-19b e miR-20a on TF expression of unpooled peripheral blood mononuclear cells (PBMCs) suspensions exposed to HG and lipopolysaccharide (LPS), per se and in presence of Olmesartan (OLM), an AT1R antagonist.

Conclusions: miR-20a may potentially contribute to post-transcriptional regulation of TF in PBMCs exposed to HG and LPS and AT1R blockade although either miR-19a or miR-19b do not appear to play a significant role in that specific context. The data open insofar unexplored and potentially relevant facets to our understanding of the complex mechanisms linking ATII, inflammation and coagulation.