During the Babel Conference of 2268, she accompanied her husband aboard the USS Enterprise, and helped Sarek and Spock to reconcile some of their differences. Spock wondered why his father would marry an emotional woman; Sarek replied it seemed the logical thing to do at the time, a comment that Amanda found quite charming. (TOS: "Journey to Babel")

During this journey, CaptainKirk was at a loss on how to properly refer to her, calling her "Mrs. Sarek." Amanda said that her married name was usually unpronounceable by Humans, although she could do it, "after a fashion, and with many years of practice." She said to simply call her "Amanda." (TOS: "Journey to Babel") In the Vulcan society, she was referred to as "the Lady Amanda." (TAS: "Yesteryear")

The script of "Journey to Babel" includes a description of Amanda that reads, "She's in her late fifties and still a fascinating woman... straight, slim, humor and warmth still alive in her... and guts. She married a Vulcan and came to live on his world where her human-woman emotions had no place. She has accepted every bit of the unemotionalism Vulcan could dish out with no loss of her own warmth and human caring... but it has had to be buried inside, in deference to her husband's customs and world."

Unlike the character of Sarek, Amanda Grayson was not included in Star Trek III: The Search for Spock and is absent from the resurrection of Spock that takes place in the conclusion of that film. Her exclusion from the movie is because the production staff couldn't find a way to feature her in the story without her presence seeming overly sentimental. Executive Producer Harve Bennett reckoned, "All she would have contributed was sympathy. The economy of the story was that Kirk and crew get Spock back. Family is secondary. That would have depreciated the moment when Spock says, 'Your name is Jim.' Then we'd have to cut to mother and she would say, 'Oh my God, he speaks!'" (The Making of the Trek Films, p. 46; Trek: The Unauthorized Story of the Movies, 3rd ed., pp. 87-88)

Once, when Sarek actor Mark Lenard was asked where Amanda was in the Star Trek movies, he replied, "In the kitchen! Where else would a good Vulcan wife be?" (Cinefantastique, Vol. 27, No. 11/12, p. 103)

Amanda died sometime between Star Trek IV and TNG: "Sarek", as Sarek had remarried to Perrin by the time of that episode's setting.

The novelization of TAS: "Yesteryear" (as published in Star Trek Log 1) describes Amanda in an image of her from circa 2237 (i.e. shortly before her death in that episode) as being pictured "in her early thirties." This roughly matches the fact that, in the novel Sarek, her year of birth is established as being 2202.

The Enterprise Log 3 collection from Golden Press lists her maiden name as "Amanda Druce", and her birthplace as "New Chicago", daughter of "Melvin Druce", an interstellar trader-explorer.

The TOS novel Ishmael cites Amanda Grayson as being a descendant of Aaron Stempel, a lead character on the real-world television series Here Come the Brides. On this show, the character of Aaron Stempel was played by Mark Lenard, the actor who played Sarek in Star Trek.