Whole genome pooled library distribution

eagle-i ID

Resource Type

Properties

"The Resource has prepared pools of plasmid DNA for clones from the TRC shRNA library by species and produced concentrated viral vector (³5x108 TU/ml) for infection of mammalian cell cultures. Each plasmid DNA/viral vector pool consists of ~9600 constructs with the whole genome of each species (human and mouse) covered by 12 pools, respectively. The pools are not organized by GO annotated gene families. Each viral shRNA vector pool and control vector (e.g. empty, non-targeting shRNA) will be provided as 2 x 10ul frozen aliquots in PBS that have been verified to be at least 5 x 108 TU/ml, as determined using a p24 assay."