Bottom Line:
The transdermal permeation and skin partitioning of from optimized formulation was significantly higher (P < 0.05) as compared to plain drug and plain gel formulation which is due to presence of surfactant acting as permeation enhancer.Permeation of optimized formulation was found to be about 4.7 times higher than plain drug gel.Significant reduction of edema (P < 0.10) was observed in comparison to the commercial product.

ABSTRACTThe purpose of study is to formulate and evaluate ufasomal gel of dexamethasone. Ufasomal suspension was made by sonication method using different concentrations of Span 80, Span 20 and cholesterol along with 25 mg of drug. Ufasomal gel was formulated by hydration method using carbopol 940. Ufasomal vesicles appeared as spherical and multilamellar under Transmission Electron Microscope. Ufasomal formulation prepared with drug to oleic acid molar ratio 8:2 (UF-2) produced greater number of vesicles and greater entrapment efficiency. UF-2 was optimized for further evaluation. The transdermal permeation and skin partitioning of from optimized formulation was significantly higher (P < 0.05) as compared to plain drug and plain gel formulation which is due to presence of surfactant acting as permeation enhancer. Permeation of optimized formulation was found to be about 4.7 times higher than plain drug gel. Anti-inflammatory activity evaluated by inhibition Carrageenan induced rat paw edema model. Significant reduction of edema (P < 0.10) was observed in comparison to the commercial product. Hence oleic acid based vesicles can be used as alternate carrier for topical delivery.

Mentions:
In vitro release behavior of dexamethasone from vesicular formulations containing oleic acid and Span 20 was investigated using locally fabricated Franz glass diffusion cell and through the cellophane membrane (Molecular weight cut of 12,000–14,000, HI Media, Ltd.). Pure dexamethasone was dissolved in PBS (pH 7.4.) at a concentration of 1 mg/mL and used as control. The prepared complex was dissolved in PBS (pH 7.4) at a concentration of 1 mg/mL (the same concentration of dexamethasone as 1 mg/mL pure drug solution). This solution (2 mL in volume) was transferred to a dialysis bag (size cut off = 2.5 mm) immediately. The dialysis bag was placed in a 50 mL-beaker containing 40 mL PBS (pH 7.4). The outer phase was stirred continuously. At predetermined time intervals sample was withdrawn and replenished with same amount of receptor fluid. The absorbance of the outer phase was monitored at 241 nm spectrophotometrically in order to characterize the concentration of dexamethasone (Figure 3).

Mentions:
In vitro release behavior of dexamethasone from vesicular formulations containing oleic acid and Span 20 was investigated using locally fabricated Franz glass diffusion cell and through the cellophane membrane (Molecular weight cut of 12,000–14,000, HI Media, Ltd.). Pure dexamethasone was dissolved in PBS (pH 7.4.) at a concentration of 1 mg/mL and used as control. The prepared complex was dissolved in PBS (pH 7.4) at a concentration of 1 mg/mL (the same concentration of dexamethasone as 1 mg/mL pure drug solution). This solution (2 mL in volume) was transferred to a dialysis bag (size cut off = 2.5 mm) immediately. The dialysis bag was placed in a 50 mL-beaker containing 40 mL PBS (pH 7.4). The outer phase was stirred continuously. At predetermined time intervals sample was withdrawn and replenished with same amount of receptor fluid. The absorbance of the outer phase was monitored at 241 nm spectrophotometrically in order to characterize the concentration of dexamethasone (Figure 3).

Bottom Line:
The transdermal permeation and skin partitioning of from optimized formulation was significantly higher (P < 0.05) as compared to plain drug and plain gel formulation which is due to presence of surfactant acting as permeation enhancer.Permeation of optimized formulation was found to be about 4.7 times higher than plain drug gel.Significant reduction of edema (P < 0.10) was observed in comparison to the commercial product.

ABSTRACTThe purpose of study is to formulate and evaluate ufasomal gel of dexamethasone. Ufasomal suspension was made by sonication method using different concentrations of Span 80, Span 20 and cholesterol along with 25 mg of drug. Ufasomal gel was formulated by hydration method using carbopol 940. Ufasomal vesicles appeared as spherical and multilamellar under Transmission Electron Microscope. Ufasomal formulation prepared with drug to oleic acid molar ratio 8:2 (UF-2) produced greater number of vesicles and greater entrapment efficiency. UF-2 was optimized for further evaluation. The transdermal permeation and skin partitioning of from optimized formulation was significantly higher (P < 0.05) as compared to plain drug and plain gel formulation which is due to presence of surfactant acting as permeation enhancer. Permeation of optimized formulation was found to be about 4.7 times higher than plain drug gel. Anti-inflammatory activity evaluated by inhibition Carrageenan induced rat paw edema model. Significant reduction of edema (P < 0.10) was observed in comparison to the commercial product. Hence oleic acid based vesicles can be used as alternate carrier for topical delivery.