Immunohistochemistry: 1:75-1:150. This antibody can be used on frozen tissue sections or formalin-fixed paraffin embedded tissue sections (requires pretreatment with trypsin or high temperature antigen unmasking using 10 mM Citrate buffer pH 6.0 for 10-20 min followd by cooling to RT for 10-20 mins; Prolonged fixation in buffered formalin can destroy the Epitope; Recommended positive control: Heart Muscle, Smooth and Skeletal Muscle, Leiomyoma and Rhabdomyomas). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.

Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.

Aliquoting Instructions: Do not dilute the entire reconstituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4°C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.

Format

State:

Ig Fraction

Specificity

Specificity:

Desmin is the main intermediate filament in mature skeletal, cardiac, and smooth muscle cells. In immunoblots, this antibody reacts with a 53 kD polypeptide. This antibody cross-reacts with most mammalian desmins. It does not react with vimentin or human cytokeratins.Reacts with Human, Porcine. Others not tested.