Background In modern biotechnology, there is a need for pausing cell lines by cold storage to adapt large-scale cell cultures to the variable demand for their products. We compared various cell culture media/solutions for cold storage of Vero-B4 kidney cells, a cell line widely used in biotechnology. Results Cold storage in RPMI 1640 medium, a r...

Background In modern biotechnology, there is a need for pausing cell lines by cold storage to adapt large-scale cell cultures to the variable demand for their products. We compared various cell culture media/solutions for cold storage of Vero-B4 kidney cells, a cell line widely used in biotechnology. Results Cold storage in RPMI 1640 medium, a recommended cell culture medium for Vero-B4 cells, surprisingly, strongly enhanced cold-induced cell injury in these cells in comparison to cold storage in Krebs-Henseleit buffer or other cell culture media (DMEM, L-15 and M199). Manufacturer, batch, medium supplements and the most likely components with concentrations outside the range of the other media/solutions (vitamin B12, inositol, biotin, p-aminobenzoic acid) did not cause this aggravation of cold-induced injury in RPMI 1640. However, a modified Krebs-Henseleit buffer with a low calcium concentration (0.42 mM), a high concentration of inorganic phosphate (5.6 mM), and glucose (11.1 mM; i.e. concentrations as in RPMI 1640) evoked a cell injury and loss of metabolic function corresponding to that observed in RPMI 1640. Deferoxamine improved cell survival and preserved metabolic function in modified Krebs-Henseleit buffer as well as in RPMI 1640. Similar Ca2+ and phosphate concentrations did not increase cold-induced cell injury in the kidney cell line LLC-PK1, porcine aortic endothelial cells or rat hepatocytes. However, more extreme conditions (Ca2+ was nominally absent and phosphate concentration raised to 25 mM as in the organ preservation solution University of Wisconsin solution) also increased cold-induced injury in rat hepatocytes and porcine aortic endothelial cells. Conclusion These data suggest that the combination of low calcium and high phosphate concentrations in the presence of glucose enhances cold-induced, iron-dependent injury drastically in Vero-B4 cells, and that a tendency for this pathomechanism also exists in other cell types. Minimize

Abstract Background In modern biotechnology, there is a need for pausing cell lines by cold storage to adapt large-scale cell cultures to the variable demand for their products. We compared various cell culture media/solutions for cold storage of Vero-B4 kidney cells, a cell line widely used in biotechnology. Results Cold storage in RPMI 1640 me...

Abstract Background In modern biotechnology, there is a need for pausing cell lines by cold storage to adapt large-scale cell cultures to the variable demand for their products. We compared various cell culture media/solutions for cold storage of Vero-B4 kidney cells, a cell line widely used in biotechnology. Results Cold storage in RPMI 1640 medium, a recommended cell culture medium for Vero-B4 cells, surprisingly, strongly enhanced cold-induced cell injury in these cells in comparison to cold storage in Krebs-Henseleit buffer or other cell culture media (DMEM, L-15 and M199). Manufacturer, batch, medium supplements and the most likely components with concentrations outside the range of the other media/solutions (vitamin B 12 , inositol, biotin, p-aminobenzoic acid) did not cause this aggravation of cold-induced injury in RPMI 1640. However, a modified Krebs-Henseleit buffer with a low calcium concentration (0.42 mM), a high concentration of inorganic phosphate (5.6 mM), and glucose (11.1 mM; i.e. concentrations as in RPMI 1640) evoked a cell injury and loss of metabolic function corresponding to that observed in RPMI 1640. Deferoxamine improved cell survival and preserved metabolic function in modified Krebs-Henseleit buffer as well as in RPMI 1640. Similar Ca 2+ and phosphate concentrations did not increase cold-induced cell injury in the kidney cell line LLC-PK 1 , porcine aortic endothelial cells or rat hepatocytes. However, more extreme conditions (Ca 2+ was nominally absent and phosphate concentration raised to 25 mM as in the organ preservation solution University of Wisconsin solution) also increased cold-induced injury in rat hepatocytes and porcine aortic endothelial cells. Conclusion These data suggest that the combination of low calcium and high phosphate concentrations in the presence of glucose enhances cold-induced, iron-dependent injury drastically in Vero-B4 cells, and that a tendency for this pathomechanism also exists in other cell types. Minimize

Perfectly synchronous systems immediately react to the inputs of their environment. These instantaneous reactions may result in so-called causality cycles between the actions of a system and their preconditions. Programs with causality cycles may or may not have consistent and unambiguous behaviors. For this reason, compilers have to perform a c...

Perfectly synchronous systems immediately react to the inputs of their environment. These instantaneous reactions may result in so-called causality cycles between the actions of a system and their preconditions. Programs with causality cycles may or may not have consistent and unambiguous behaviors. For this reason, compilers have to perform a causality analysis before code generation. In this paper, we analyze the impact of different code generation schemes on causality analysis and propose translations that yield different degrees of causality. To this end, we first translate the program to an equation system as an intermediate representation, which may alternatively be viewed as a hardware circuit. The second step then analyzes the equation system as known from ternary simulation of hardware circuits with combinational feedback loops. In particular, we consider alternative ways to obtain logically equivalent equation systems that show, however, different results in causality analysis. Minimize

Perfectly synchronous systems immediately react to the inputs of their environment. These instantaneous reactions may result in so-called causality cycles between the actions of a system and their preconditions. Programs with causality cycles may or may not have consistent and unambiguous behaviors. For this reason, compilers have to perform a c...

Perfectly synchronous systems immediately react to the inputs of their environment. These instantaneous reactions may result in so-called causality cycles between the actions of a system and their preconditions. Programs with causality cycles may or may not have consistent and unambiguous behaviors. For this reason, compilers have to perform a causality analysis before code generation. In this paper, we analyze the impact of different code generation schemes on causality analysis and propose translations that yield different degrees of causality. To this end, we first translate the program to an equation system as an intermediate representation, which may alternatively be viewed as a hardware circuit. The second step then analyzes the equation system as known from ternary simulation of hardware circuits with combinational feedback loops. In particular, we consider alternative ways to obtain logically equivalent equation systems that show, however, different results in causality analysis. Minimize

Perfectly synchronous systems immediately react to the inputs of their environment, which may lead to so-called causality cycles between actions and their trigger conditions. Algorithms to analyze the consistency of such cycles usually extend data types by an additional value to explicitly indicate unknown values. In particular, Boolean function...

Perfectly synchronous systems immediately react to the inputs of their environment, which may lead to so-called causality cycles between actions and their trigger conditions. Algorithms to analyze the consistency of such cycles usually extend data types by an additional value to explicitly indicate unknown values. In particular, Boolean functions are thereby extended to ternary functions. However, a Boolean function usually has several ternary extensions, and the result of the causality analysis depends on the chosen ternary extension. In this paper, we show that there always is a maximal ternary extension that allows one to solve as many causality problems as possible. Moreover, we elaborate the relationship to hazard elimination in hardware circuits, and finally show how the maximal ternary extension of a Boolean function can be efficiently computed by means of binary decision diagrams. Minimize

Perfectly synchronous systems immediately react to the inputs of their environment, which may lead to so-called causality cycles between actions and their trigger conditions. Algorithms to analyze the consistency of such cycles usually extend data types by an additional value to explicitly indicate unknown values. In particular, Boolean function...

Perfectly synchronous systems immediately react to the inputs of their environment, which may lead to so-called causality cycles between actions and their trigger conditions. Algorithms to analyze the consistency of such cycles usually extend data types by an additional value to explicitly indicate unknown values. In particular, Boolean functions are thereby extended to ternary functions. However, a Boolean function usually has several ternary extensions, and the result of the causality analysis depends on the chosen ternary extension. In this paper, we show that there always is a maximal ternary extension that allows one to solve as many causality problems as possible. Moreover, we elaborate the relationship to hazard elimination in hardware circuits, and finally show how the maximal ternary extension of a Boolean function can be efficiently computed by means of binary decision diagrams. Minimize

Background: Serum creatinine (SCR) and blood urea nitrogen (BUN) determine the glomerular filtration rate (GFR) improperly in acute renal failure. Serum cystatin C (CYS) has the potential to be a more precise marker for GFR. The aim of this study was to compare the sensitivity of SCR, BUN and CYS with respect to the detection of acute renal fai...

Background. Serum creatinine (SCR) and blood urea nitrogen (BUN) determine the glomerular filtration rate (GFR) improperly in acute renal failure. Serum cystatin C (CYS) has the potential to be a more precise marker for GFR. The aim of this study was to compare the sensitivity of SCR, BUN and CYS with respect to the detection of acute renal fai...