Atmospheric pollen and mold counts -
I am experienced with atmospheric pollen and mold identification and total counts per cubic meter using the Anderson air sampler and impellers. I used these techniques while employed at Hendrick Medical Center in Abilene, TX. The hospital reported the counts to a local television station as a public service and for media exposure.

Clinical Laboratory Management -
I have served as a microbiology supervisor and as a microbiology manager at previous employers. I have experience in staffing and scheduling, budget preparation, employee hiring, counseling and dismissal, development of performance improvement plans, supply requisition, client relationships, and logistics. I consult on many of the above categories in my role as the Director of Microbiology at University Hospital.

Clinical Laboratory Science -
I am certified as MT(ASCP) as a generalist in medical technology (clinical laboratory science). I maintain a basic level of expertise in all aspects of laboratory science.

Clinical Microbiology -
I am certified in medical and public health microbiology by the American Board of Medical Microbiology (ABMM). I am also certified as SM(ASCP), specialist in microbiology. I have expertise in all aspects of clinical microbiology to including bacteriology, mycology, mycobacteriology, virology, parasitology, and molecular microbiology. My expertise includes automated and manual identification and susceptibility testing, instrumentation and rapid detection of pathogens by molecular methods.

Detection and characterization of glycoproteins. -
I have experience with detection, characterization and manipulation of glycoproteins of human milk and their interactions with bacterial toxins and gut flora.

Detection of proteins -
I have experience in detection of proteins and antigen-antibody interactions by standard and chemiluminescent Western blots. I developed several enzyme immunoassays for bacterial toxins, immunoglobulins, and secretory component.

Development of ELISA techniques -
While in graduate school, I developed ELISA techniques and established linearity, sensitivity and specificity for analytes.

FDA In-vitro device clinical trials -
I have administered two successful in-vitro device clinical trials with a laboratory diagnostics company. These trials led to FDA approval of the in-vitro device and to international marketing of the products.

Handling of small laboratory animals -
While in graduate school I was trained and certified by TTUHSC to handle laboratory animals including intubation, anesthesia, abdominal surgery and suture, and establishment of lethal toxin levels.

Purification of bacterial toxins -
I have experience in purifying gram amounts of bacterial toxins, specifically Clostridium difficile toxins A and B.

Radiolabeling and autoradiography of proteins -
I have experience with radiolabeling bacterial toxins with Iodine 125, while maintaining the potency of the toxin.

Teaching Clinical Microbiology -
I have many years of teaching clinical microbiology in the hospital laboratory setting, in the classroom, and in group laboratories. I also teach management, infectious diseases, pathology, and global health. This teaching includes dental students, PA students, infectious diseases fellows, pathology residents and fellows, and pharmacy residents.

Transmission electron microscopy, bacteriophage -
I have experience with transmission electron microscopy of bacteria and bacteriophage, including specimen preparation, film processing and print making techniques.

Review Article

Teaching

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Date

Description

Institution

# Students

5/2014 - Present

Parasitology/Mycology Lecture

The University of Texas Health Science Center

30 students

Course Director - In 2014 CLSC 3004 was transformed into CLSC 3040 "Special Topics in Microbiology" but the new course is not listed yet. I designed and organized the new course. Parasitology and Mycology are taught and anaerobic microbiology and acid fast bacilli were added to the curriculum. I prepared all new didactic lectures and emphasized case studies, group activities, learning drills and motion games. This is a recurring course and the average enrollment is 30.

5/2014 - Present

Parasitology/Mycology Lab

The University of Texas Health Science Center

30 students

Course Director - Note: In 2014 CLSC 3003 laboratory was transformed into CLSC 3040, "Special Topics in Microbiology" which added anaerobic microbiology and acid fast bacilli to the former curriculum of parasititology and mycology. CLSC 3040 is not listed in the drag down box. I designed and organized this course with key help from Tish Barnhart, CLS laboratory manager. The laboratory emphasized common microbes seen in the specialty sub-disciplines of microbiology which tend to be neglected or unavailable in clinical practicums. We were able to demonstrate anaerobic microbes and acid fast bacilli for the first time ever due to new equipment. The course was a success and student comments were very positive. We will optimize the course next year for better logistical flow. This is a recurring course and the average enrollment is 30.

3/2011 - Present

Clinical Pathology

The University of Texas Health Science Center

16 students

I teach a group of 16 fourth year medical students for three sessions of Clinical Applications of Laboratory Medicine (CALM). I assist with a small group activity with fourth year medical students reviewing and solving four microbiology/antimicrobial treatment cases. This is a recurring course and the average enrollment is 16. Ongoing through 2016.

1/2010 - Present

Dental Microbiology

The University of Texas Health Science Center

120 students

In preparation for the lecture, I completely revised the outdated microbiology handout and added numerous images. I teach a two hour didactic lecture on clinical parasitology to the first year dental students with emphasis on current and rapid diagnostics, specimen collection, and parasites commonly seen in USA and returning travelers abroad. I include actual preserved parasites as a visual aid. The lecture includes short case vignettes as teaching points. I submit three multiple questions for the final examination. This is a recurring course and the average enrollment is 120.

1/2010 - Present

Laboratory Medicine

The University of Texas Health Science Center

42 students

I teach three didactic lectures to Physician Assistant students regarding specimen collection, direct specimen examination, the interpretation of microbiology results, and parasitology. The didactic time includes demonstrations, group activities, quizzes and a practical exercise in the use of an antibiogram to choose appropriate initial antimicrobial therapy. In 2014 I added a small group exercise which taught the use of a hospital antibiogram to guide antimicrobial therapy. This is a recurring course and the average enrollment is 40.

10/2009 - Present

Clinical Microbiology

The University of Texas Health Science Center

12 students

I guide daily and weekly "plate rounds" for pathology residents, pharmacy residents, podiatry residents, and infectious disease fellows. This teaching is centered at the University Hospital Microbiology Laboratory. I participate in the weekly pathology noon conference and present cases. I present three didactic one hour lectures on microbiology topics for pathology resident board examination preparation. I routinely assist pathology residents with microscopic images and data collection for their case presentations. This is a recurring course 2009 to present with 15 pathology residents on average.

9/2009 - Present

Adv Microbiology

The University of Texas Health Science Center

30 students

Course Director - I am the course director for CLSC 4033 Advanced Microbiology, a senior-level undergraduate course. I design, organize, and evaluate this course. I invite one or two guest speakers who are clinical pharmacists at University Hospital. The course is a body-site, organ-system based approach to the detection, identification, and eradication of pathogenic microbes. The course emphasizes group-based case learning, using real cases from University Hospital. The course also emphasizes current epidemics and outbreaks and emerging infectious diseases and public health measures. Hospital infection control cases are also presented. This is a recurring course and the average enrollment is 30. This course prepares students to enter their clinical practicums.

1/2009 - Present

Medical Microbiology Lab

The University of Texas Health Science Center

25 students

Course Director - I am the course director for the hands-on medical/clinical laboratory for junior CLS students including detection and identification of microbes in human specimens by microscopy, culture and biochemical methods. Basic unknowns and interpretation of pure and mixed cultures by relevant body site are emphasized. Antimicrobial susceptibility testing is presented by both manual and automated techniques. In 2014 a BioMerieux Vitek 2 instrument was added to the laboratory, allowing the teaching of instrumentation currently in use in 70% of laboratories in the USA. I design, organize, and evaluate the effectiveness of this course. This is a recurring course and the average enrollment is 25.

1/2009 - Present

Medical Microbiology

The University of Texas Health Science Center

25 students

Course Director - CLSC 3033 is the didactic course introducing medical/clinical microbiology for first year (junior) CLS students. I am responsible for the design, organization, and evaluation of this course. The course emphasizes pathogens and normal flora by body site. the details of microscopy, culture work-up, organism virulence characteristics, susceptibility testing, rapid detection methods, culture reporting, and the interpretation of clinical microbiology results are analyzed in the context of well and sick individuals. Tropical and neglected disease in third-world countries are also discussed. Small group activities feature the use of antibiograms, antimicrobial selection, and resistance mechanisms.
Ongoing, Spring 2009, Spring 2010, 2011, 2012, 2013, 2014. This is a recurring course and the average enrollment is 20 to 30 students.

CMI CF-301 Lysin vs. Staphylococcus aureus Survey Collection. I reviewed and collected MRSA and MSSA isolates from University Hospital for the study specified below. The study will pay $1250 to my T account and will later share conclusions from the multi-site study.

CMI is collecting 50 strains of Staphylococcus aureus from each of six US laboratories, three European laboratories and 2 laboratories in South America. These strains will be used to determine the susceptibility of a new lysin compound vs. 25 S. aureus oxacillin susceptible and 25 S. aureus oxacillin resistant obtained from each of the 10 sites.
Sites will collect all strains from recent clinical isolates isolated in 2016. Frozen stocks may be included as long as all cultures were collected in 2016 or early 2017.
Each site should collect 25 MRSA and 25 MSSA each isolate should be from a unique patient culture. Only one isolate per patient should be included. Please provide the date cultured and the culture source for each isolate (See Excel file for recording this information). If mecA or PBP 2a test results are available please include the test and the result on the worksheet.

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