Bottom Line:
Moreover, the decrease in SIRT1 activity with special inhibitor EX527 had a synergic effect on chemotherapy with gemcitabine in PANC-1 and ASPC-1 cell lines, which significantly promoted apoptosis, senescence, and G0 /G1 cycle arrest.Western blot results also showed that SIRT1, acetylated-p53, FOXO3a, and p21 were upregulated after combined treatment, whereas no obvious change was evident in total p53 protein.Thus, our results indicated a special role for SIRT1 in the regulation of adaptive response to chemotherapy-induced stress, which is involved in chemoresistance.

Mentions:
According to the results of the SA-β-gal assay, compared to untreated PANC-1 and ASPC-1 cells, SA-β-gal-positive cells were mildly increased in GEM-treated PANC-1 and ASPC-1 cells, and moderately increased in EX527-treated cells. Moreover, compared to GEM or EX527 alone, the combination of GEM and EX527 significantly increased the number of SA-β-gal-positive cells in PANC-1 and ASPC-1 cells even further (Fig. 7).

Mentions:
According to the results of the SA-β-gal assay, compared to untreated PANC-1 and ASPC-1 cells, SA-β-gal-positive cells were mildly increased in GEM-treated PANC-1 and ASPC-1 cells, and moderately increased in EX527-treated cells. Moreover, compared to GEM or EX527 alone, the combination of GEM and EX527 significantly increased the number of SA-β-gal-positive cells in PANC-1 and ASPC-1 cells even further (Fig. 7).

Bottom Line:
Moreover, the decrease in SIRT1 activity with special inhibitor EX527 had a synergic effect on chemotherapy with gemcitabine in PANC-1 and ASPC-1 cell lines, which significantly promoted apoptosis, senescence, and G0 /G1 cycle arrest.Western blot results also showed that SIRT1, acetylated-p53, FOXO3a, and p21 were upregulated after combined treatment, whereas no obvious change was evident in total p53 protein.Thus, our results indicated a special role for SIRT1 in the regulation of adaptive response to chemotherapy-induced stress, which is involved in chemoresistance.