Gimenez stain yields a clear cut staining of rikketsiae, which appears as pink/red whereas the underlying tissue is blue/green.

Although, Gimenez stain has been used to stain the various rickettsial bacteria such as Bartonella, Coxiella, etc (20), it has not been reported to be used for the detection of Wolbachia from mosquito ovaries.

The bacterium was, however, isolated in guinea pigs and in the yolk sacs of embryonated hens' eggs and was visualized by Gimenez stain during one of several efforts to isolate Q fever rickettsiae (Coxiella burnetii) from specimens of lung tissue collected at autopsy.

Chlamydophila was detected by PCR and Gimenez stain of the splenic samples, but the extent of hepatic necrosis and the general absence of Chlarnydophila in necrotic lesions in the spleen and liver suggests that other agents may have contributed to the lesions, possibly reovirus.

Gimenez stains for the demonstration of chlamydial bodies were positive in 3 of 6 birds, although the number of infected cells was considered very low in comparison with birds that typically die from chlamydiosis.

Antigens were prepared by placing the rickettsial stains in L929 cells and HL60 or and DH82 cells, respectively; collecting the culture when Gimenez stain or Wright staining showed positive results; ultrasonically crushing the culture; and purifying the bacteria by density ultracentrifugation.

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