Development conditions: The solvent is allowed to migrate until the front reaches a line 15 cm from the starting line.

Detection: The plate is examined under ultraviolet light at a wavelength of maximum output at about 254 nm and 365 nm, and then left in a chromatographic chamber containing some iodine crystals until spots appear.

Results: The spots obtained from the solution of the sample subjected to procedure A and the spots obtained from the reference solution of ergometrine hydrogen maleate display the same chromatographic characteristics of migration and detection. After procedure B, two additional spots are obtained and some tailing.