Reduction of the minimal sequence for initiation of DNA synthesis by qualitative or quantitative changes of an initiator protein.

Details

Reduction of the minimal sequence for initiation of DNA synthesis by qualitative or quantitative changes of an initiator protein.

Journal

Nature

Author(s)

DottoG.P., ZinderN.D.

ISSN

0028-0836 (Print)

ISSN-L

0028-0836

Publication state

Published

Issued date

1984

Volume

311

Number

5983

Pages

279-280

Language

english

Abstract

Initiation of DNA synthesis at an origin of DNA replication involves complex protein-DNA interactions that are still poorly understood. Some of these interactions are highly specific and involve proteins (initiator proteins) thought to be essential for regulation of the initiation process because of their rate-limiting activity. We show here that both qualitative and quantitative changes in one of these proteins have profound effects on protein-DNA interactions at an origin of DNA replication, and are sufficient to reduce to less than one-third the minimal sequence required for initiation. The general implications of these findings are discussed.