I want a metabolic label to be incorporated into proteins or other cellular structures by adding the reagent to the media of cultured cells or in vivo injection. What are my options?

The incorporation of azide-modified biomolecules permits eventual detection or crosslinking using alkyne or phosphine reagents. The incorporation of alkyne-modified biomolecules allows eventual detection or crosslinking using azide-modified reagents. The incorporation of either EdU or EU requires that the cells/tissues possess a pyrimidine salvage pathway.

Can I perform Click-iT EdU detection on cells growing in 3D culture?

We have not validated the use of EdU for proliferation in 3D culture systems, but as this reagent is compatible for labeling cells in vivo, it is also expected to label cells in 3D culture systems. There are a number of reports in the literature that use this product in 3D culture systems; here are some citations:

Can I perform Click-iT EdU TUNEL detection on cells growing in 3D culture?

We have not validated the use of EdU TUNEL for apoptosis detection in 3D culture systems, but as this reagent is compatible for labeling cells in vivo, it is also expected to label cells in 3D culture systems. There are a number of reports in the literature that use this product in 3D culture systems; here are some citations: