Card Range To Study

12 Cards in this Set

Front

Back

What are the 6 laboratory operating procedures each student is responsible for doing?

1) arrive on time
2) read all exercises adn appendices before lab
3) care properly for microscope
4) keep all equipment in food working condition and stored properly
5) dispose of items properly
6) turn off water faucets and gas burners when not in use or when leaving bench area

What are the proper steps for microscope care?

-put 4x objective over stage hole
-center specimen clamps over stage
-clean all lenses
-fold cord and place under condenser
-remove all traces of oil from objective housings and stage
-place cover on microscope

What waste is put in the 'Waste Paper Basket'?

-any used paper towels that are NOT contaminated

What waste is put in the 'Red Sharps Container'?

-small pieces of contaminated glass like slides

What waste is put in the 'Loafpands or Tri-pours'?
What is the liquid in these?

What waste is put in the 'Lidded Trash Cans'?
What is used to line these cans?

-contaminated kimwipes, paper towels and petri dishes
-Biohazard Bags

What waste is put in the 'Test tube rack marked for discard'?

-contaminated screw cap or slip top tubes

What are the 5 proper culture-handling procedures students are responsible for?

1) wipe benchtops with disinfectant before and after
2) flame contaminated inoculating tools before putting on benchtop
3) use test tube racks, baskets, or other designated containers to carry/store cultures/media
4) never pipette by mouth, or put contaminated ones on bench. DISCARD into loaf pans or tri-pours (glass in sharps)
5) In case of accident remain calm and carry out proper procedures

What are are the proper procedures in the case of an accident or spill in lab?

-report accident to instructor immediately
-place paper towels over spilled material
-pour disinfectatnt liberally over towels
-wait 5 minutes and dispose of towels in lidded can with biohazard bag. If glass if involved, leave this to TA
-once towels are gone, pour disinfectant over area and wipe up again
-if you spill on yourself, pour disinfectant on area and tell instructor