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The route taken by papillomaviruses from the cell surface to the nucleus during infection is incompletely understood. for localization of L2 and viral DNA to the Golgi apparatus and the endoplasmic reticulum. These results show that incoming HPV16 traffics sequentially from the cell surface to the endosome and then to the Golgi apparatus and the endoplasmic reticulum prior to nuclear entry. IMPORTANCE The human papillomaviruses are small nonenveloped DNA viruses responsible for approximately 5% of all human cancer deaths, but little is known about the process by which these viruses transit from the cell surface to the 48449-76-7 supplier nucleus. Here we show that incoming HPV16, the most common high-risk HPV, traffics though a series of vesicular compartments during infectious entry, including the endosome, Golgi apparatus, and endoplasmic reticulum. Furthermore, we show that -secretase, a cellular membrane-associated protease, is required for entry of the L2 minor capsid protein and viral DNA into the Golgi apparatus and endoplasmic reticulum. These studies reveal a new pathway of cell entry by DNA viruses and suggest that Rabbit Polyclonal to EMR2 components of this pathway are candidate antiviral targets. INTRODUCTION Papillomaviruses are important pathogens. The high-risk human papillomaviruses (HPVs), such as HPV16, play an etiologic role in essentially all cervical cancers and in a substantial fraction of other anogenital and oropharyngeal cancers (1, 2). Despite the importance of 48449-76-7 supplier 48449-76-7 supplier these viruses, relatively little is known about the crucial process of HPV entry. Papillomaviruses are nonenveloped DNA viruses. HPV capsids are composed of 360 molecules of the major capsid protein L1 and up to 72 molecules of the minor 48449-76-7 supplier capsid protein L2. Studies of HPV entry have been facilitated by the use of pseudoviruses (PsVs) comprised of L1 plus L2 encapsidating a reporter plasmid (3, 4). These PsVs, which can be readily prepared in cultured cells, mimic the behavior of authentic virus and allow infection efficiency to be easily monitored by reporter gene expression. The use of PsVs also allows the addition of epitope tags to the viral proteins to visualize the virus during entry. After L1-mediated binding of the capsid to heparan sulfate proteoglycans at the cell surface, the capsid proteins undergo conformational changes and L2 is cleaved by the cellular protease furin (5,C14). HPV is then transferred to an internalization receptor and endocytosed (6, 7, 15,C19). After endosome entry, capsid disassembly is initiated by acidification of the endosomal lumen (16, 19,C21). HPV then travels to the nucleus, where virus replication occurs (22). Mitotic progression and nuclear envelope breakdown appear important for nuclear entry (23, 24). During virus entry, the L1 protein largely dissociates from the viral DNA, a step that requires cyclophilin B action, but the L2 protein remains associated with the viral genome and colocalizes with viral DNA at nuclear promyelocytic leukemia protein (PML) bodies (25, 26). We conducted a genome-wide small interfering RNA (siRNA) screen for cellular genes that affect the efficiency of infection by HPV16 PsV. The screen showed that retrograde trafficking is required for HPV entry (27). Further studies showed that HPV16 L1 and L2 and viral DNA travel to the Golgi apparatus in both HeLa and HaCaT cells (23, 27,C29) and that trafficking of HPV to the Golgi requires the retromer, a protein complex that initiates vesicular transport of cellular cargo from the endosome to the Golgi apparatus (27, 30, 31). The path taken by HPV from the endosome to the Golgi apparatus and from the Golgi apparatus to the nucleus is undefined. Endoplasmic reticulum (ER) components were also enriched in two independent HPV entry siRNA screens (23, 27), and knockdown of specific ER proteins and chemical inhibition of ER function inhibit infection by HPV (32, 33). Laniosz et al. reported that HPV16 L1 colocalizes with ER markers.