Abstract

Bengle (Zingiber cassumunar Roxb.) has been known as a traditional herbal drug used as analgetic, vermifuge, and carminative. Many researches showed that contained compounds in the rizhomes also have potency as antioxidant and anti-inflammatory, the insecticidal, also analgetic effect.
In order to find out the activities potency and to enrich chemical profile of Zingiber cassumunar Roxb., isolation and identification of bioactive compound from rizhome of Zingiber cassumunar Roxb. have been carried out. The powder of rizhome was mascerated with ethyl acetate as a solvent then evaporated until crude extract was obtained. Extract was fractionated by column vaccum chromatography using n-hexane : ethyl acetate ( 1 : 1) as solvents until five fraction were gained. Then all fraction were identified the component by thin layer chromatography (TLC) using n-hexane : chloroform ( 1 : 9) as the solvents. Compound giving red spot after being sprayed by 112SO4 10% was separated by TLC preparative resulting droplet of liquid like oil in the bottom of the bottle.
Analysis of the isolate has been conducted by ultraviolet-visible spectrofotometer (Uv-Vis), infrared spectrofotometer (IR), and gas chromatography-mass spectrometer (GC-MS). The four identified compound were sesquiterpene ketonic, methyl ester 11-octadecenoic acid, tetradecane, and dibuthyl ester phtalic. Activity test by Brine Shrimp Lethality Test methode showed that the extract of the rizhome and isolate have the potency as anti¬microbial agents because the value of LC50 are 79.68 ppm and 142.13 ppm.
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