Nuclear stain for HCA/HCS cell demarcation

The HCS NuclearMask™ Blue Stain is used to measure DNA content and perform cell demarcation in both live and fixed cells on high-content imaging and analysis (HCS) platforms. The versatile HCS NuclearMask™ Stains survive standard formaldehyde-based fixation and detergent-based permeabilization methods. This protocol produces sufficient stain for one 96-well plate at a staining volume of 100 μL/well.