Antibodies - Monoclonal

A murine class IgG1 monclonal antibody to the K88a fimbral adhesin of E. coli, prepared by fusion with NS-1 myeloma cells. The hybridoma supernatant was concentrated by ultrafiltration and affinity purified using Protein-G. The finished product was standardised to a concentration of 1 mg/ml by BCA assay.

A murine class IgG1 monclonal antibody to the K88b fimbral adhesin of E. coli, prepared by fusion with NS-1 myeloma cells. The hybridoma supernatant was concentrated by ultrafiltration and affinity purified using Protein-G. The finished product was standardised to a concentration of 1 mg/ml by BCA assay.

A murine class IgG3 monclonal antibody to the 987p fimbral adhesin of E. coli, prepared by fusion with NS-1 myeloma cells. The hybridoma was grown in serum free media and the supernatant was concentrated by ultrafiltration and by water removal, using polyethylene glycol crystals. The finished product was standardised to a concentration of 1 mg/ml by BCA assay.

A murine class IgG2a monclonal antibody to the K99 fimbral adhesin of E. coli, prepared by fusion with NS-1 myeloma cells. The hybridoma supernatant was concentrated by ultrafiltration and affinity purified using Protein-G. The finished product was standardised to a concentration of 1 mg/ml by BCA assay.

Bovine Antisera - Polyclonal

A comprehensive range of antisera for use in Slide Agglutination Tests for the
presumptive identification of pathogenic E.coli from cows. All E.coli
antisera contain agglutinins to both ‘O’ and ‘K’ antigens. Sera are of rabbit origin and
are presented in liquid form ready to use. Store at 4°C.

Avian Antisera - Polyclonal

A comprehensive range of antisera for use in Slide Agglutination Tests for the presumptive identification of pathogenic E.coli from birds. All E.coli antisera contain agglutinins to both 'O' and 'K' antigens. Sera are of rabbit origin and are presented in liquid form ready to use. Store at 4°C.

Porcine Antisera - Polyclonal: Polyvalent A and constituents

A comprehensive range of antisera for use in Slide Agglutination Tests for the
presumptive identification of pathogenic E.coli from pigs. All E.coli
antisera contain agglutinins to both ‘O’ and ‘K’ antigens. Sera are of rabbit origin and
are presented in liquid form ready to use. Store at 4°C.

Porcine Antisera - Polyclonal: Polyvalent B and constituents

A comprehensive range of antisera for use in Slide Agglutination Tests for the
presumptive identification of pathogenic E.coli from pigs. All E.coli
antisera contain agglutinins to both ‘O’ and ‘K’ antigens. Sera are of rabbit origin and
are presented in liquid form ready to use. Store at 4°C.

Porcine Antisera - Polyclonal: Polyvalent C and constituents

A comprehensive range of antisera for use in Slide Agglutination Tests for the
presumptive identification of pathogenic E.coli from pigs. All E.coli
antisera contain agglutinins to both ‘O’ and ‘K’ antigens. Sera are of rabbit origin and
are presented in liquid form ready to use. Store at 4°C.

Diagnostic Kits

FIMBREX kits combine latex agglutination technology with monoclonal antibody
specificity to provide rapid, easy-to-perform tests for the presence of fimbrial adhesins
on cultures of enterotoxigenic E.coli (ETEC). Coloured latex makes test results easy to
read and end-points are reached in a few minutes. Each kit is specific for one of the
fimbrial adhesins and contains disposable agglutination test cards and reagents for 60
tests performed individually, or 90 tests when performed in multiples of three. In
addition to the monoclonal antibody-coated latex, the kit also contains a control
reagent to detect non-specific agglutination, an inactivated positive control antigen,
and suspension buffer, all supplied in convenient dropper bottles; only mixing sticks
are required in order to carry out the test. Reagents are presented in liquid form and
are ready for use.

A latex agglutination test kit utilizing latex particles coated with monoclonal antibody (MAb) that detect the K88 (F4) fimbrial adhesin of cultured enterotoxigenic E. coli. The latex particles are coloured to aid visual interpretation and the MAb ensures specificity. A control latex (latex particles coated with mouse serum) identifies non-specific reactions and a positive control antigen (a killed cell preparation of K88 fimbrial adhesins) is included to ensure that the latex are valid for testing.