When Henry Randolph was diagnosed in November 2011 with ALS, his wife Jayne was there holding his hand. She stayed by his side as his partner in life and in business even as Henry's health deteriorated. He passed away in July of 2013.

"He was sick for a year and a half, but he kept working up until about a month before he died," she said. "He couldn't speak because he was on a ventilator, but he could write. I would print out e-mails for him, and he would respond by writing on them."

The Randolph's company, Randolph Associates, is a consultancy for the food and dairy industries. Founded in the 1970s by Mr. Randolph, the company works internationally ensuring food safety and proper refrigeration for milk, yogurt and ice cream. Clients include Kroger, Dean Foods (the parent company of Barber's), Chobani Yogurt and Mayfield Ice Cream. (In case you were wondering, yes, they get to eat samples.)

Randolph Associates ALS ChallengeBIRMINGHAM, Alabama--Jane Randolph and employees of Randolph Associates take the ALS Ice Bucket Challenge in memory of the company founder, Henry Randolph who died from ALS Syndrome, Thursday, Aug. 28, 2014.

Although Mrs. Randolph was a relative stranger to the dairy industry, she began working with Henry in 2007 after she retired from her job at the U.S. Pipe and Foundry Company. She caught on quickly. She credits the company's 38 employees as being "just like family," helping her learn the ropes and transition smoothly into her role as sole proprietor.

"Henry had so much knowledge. He was unbelievable. He could talk to anyone about anything, just pick up the phone and talk to anyone from the president all the way down. We miss his presence every day. I know I am biased, but he was amazing," Randolph said.

Typically, employees at Randolph Associates use ice cream buckets for testing the shelf life of samples, but on Thursday those buckets were filled with ice and water so that 13 employees, friends and family members could take the ALS Ice Bucket Challenge.

"If he were here, he would think this was hysterical,' said Sherryll White, accounting manager at Randolph Associates who participated in the Ice Bucket Challenge. Mr. Randolph personally hired White in 2006, and she worked closely with him for several years.

"He was a teacher, a mentor and a go-getter. He would be truly humbled that we were doing this in his honor," White said.

Pierce Reichle, 11, the Randolphs' grandson, said that he had seen several Ice Bucket Challenges on Instagram and was excited to participate.

"There will be a cure one day," he said. "He was a great grandfather, and I think this is a really good thing."

In the video, Mrs. Randolph challenged all of the company's clients to step up to the Ice Bucket Challenge and donate to find a cure.

"Can you imagine if they all did it? That would be huge," she said.

Randolph Associates will be making a donation to the ALS Association, though Mrs. Randolph said she's not sure exactly how much. She gave a $5,000 donation earlier this year and is considering matching that amount.

For Mrs. Randolph, Thursday's chilly tribute goes much deeper than just paying tribute to her late husband. Raising money and awareness to fight ALS and help others is what keeps her donating year to the cause after year. Already, she said, awareness of ALS is changing.

"When we would tell people Henry had ALS, many of them didn't even know what that was. For some reason, it just didn't seem to the attention or the understanding of other serious diseases," she said. "I think the Ice Bucket Challenge has changed that. All the videos, all the people donating- it may be the best thing I've ever seen."