anti-Synaptopodin (N-terminus) guinea pig polyclonal, serum

The antibody reacts specifically with the N-terminal region of synaptopodin and recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-actinin. GP94-N also reacts with a subset of exclusively telencephalic synapses.

short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles

Tested species reactivity

human, mouse

Applications

Tested applications

Tested dilutions

Immunocytochemistry (ICC)/ Immunofluorescence (IF)

assay dependent

Immunohistochemistry (IHC) - frozen

1:50 – 1:100

Immunohistochemistry (IHC) - paraffin

1:50 – 1:100 (microwave treatment recommended)

Western Blot (WB)

1:500 -1:1,000

Background

Details

The antibody reacts specifically with the N-terminus of synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin. Synaptopodin belongs to actin-binding pro-teins, it has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the corre-sponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (attributed to posttransla-tional modifications). In Western blot analysis the antibody also reacts with a 44 kD degradation fragment of synaptopodin.