CD3 (Dako Omnis)

Clone Polyclonal

Synthetic peptide from the intracellular part of the e-chain of human CD3 was coupled to bovine thyroglobulin and used for immunization. The antibody is a pan-T cell marker for identification of T cells. It is well-suited for labeling reactive T cells in tissue with lymphoid infiltrates, and for classification of T-cell neoplasms. The antibody shows a stronger labeling intensity than corresponding monoclonal antibodies to CD3, and should, generally, be preferred on formalin-fixed, paraffin-embedded tissue sections.

In Western blotting, the antibody detects bands of the expected molecular weights for CD3 antigens (2). The antibody recognizes CD3e in both a T-cell line (Jurkat) and a natural killer cell line (NK11), but does not react with lysates prepared from several B-cell lines (Raji, Ramos and JY), a myeloid cell line (U937) or a colon carcinoma cell line (Colo-205) (9).

In immunoprecipitation from Nonidet P40 lysates of surface-iodated T lymphoblasts, the antibody precipitates the g (26 kDa), d (21 kDa) and e (19 kDa) chain of the CD3 molecule, similar to the precipitation pattern seen using the well-characterized monoclonal mouse anti-human CD3, clone UCHT1 (1).

In ELISA, the antibody labels the CD3 peptide used as immunogen. See package insert for reference(s).

The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.

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