Abstract

We report laser desorption/ionization mass spectrometry using a dye-doped nanoporous polymer matrix. The nanoporous polymer matrix was fabricated through a holographic interference patterning technique. The periodically aligned nanopores in the resulting polymer matrix produced a high surface-to-volume ratio that facilitates the homogeneous cocrystallization of the matrix and an analyte (i.e., peptide in this demonstration). To generate nanostructures with further enhanced functionalities, dyes were also incorporated into the photopolymer. We demonstrate that by using the dye-doped nanoporous polymer matrix, we can identify peptides with an enhanced signal from the peptides and decreased noise from the ion fragmentation. These results indicate that the dye-doped nanoporous polymer matrix we use here can be a promising platform for laser desorption/ionization mass spectrometry.

Mass spectra of [Glu1]-fibrinopeptide B human (MW 1570.57) obtained by using (a) nanoporous polymer film doped with C456, (b) nanoporous polymer film doped with C456 and trifluoroacetic acid, and (c) nonporous polymer film doped with C456 and trifluoroacetic acid. The inset shows the enlarged high mass region from 1400 to 1800 m/z.

Mass spectra of control samples. (a) Mass spectra of peptide using nanoporous polymer film that does not contain C456. (b) Mass spectra of peptide using C456-doped nanoporous polymer film that does not contain trifluoroacetic acid.

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