Aspirate the destaining solution and replace with fresh solution every 30 minutes and wash for 4 hours or until all traces of Coomassie have been removed. Residual amounts of Coomassie and detergents (e.g., SDS, MOPS, etc.) will partially inhibit trypsin digestion. Also, the Coomassie will co-extract with the peptides.

After the final aspiration, add 100µl acetonitrile to dehydrate the gel pieces. Aspirate the liquid. Air-dry for 5 minutes.