ABSTRACT:The present study was undertaken to evaluate the effectiveness of structured teaching program regarding collection of cord blood for stem cell therapy in terms of knowledge among staff nurses in selected hospitals at Bangalore. Pre-experimental design, with convenient sampling method was used. Information was collected from 50 staff nurses regarding collection of cord blood for stem cell therapy using the structured knowledge questionnaire. STP was implemented and post-test was conducted after 7 days to find the effectiveness. Study findings showed that pre-test knowledge scores were found to be 43.05% and after STP the posttest knowledge scores of staff nurses was found to be 77.45% which is enhanced by 34.4%. With the t-test value 25.96* and chi square value 80.18* which is significant at 5% level. Hence the result has proved that STP was effective in improving the knowledge of staff nurses on collection of cord blood for stem cell therapy.