FBXL11 (KDM2A) Homogeneous Assay Kit

FBXL11, also known as KDM2A, JHDM1A, and Ndy2, is a
histone lysine demethylase that exhibits demethylation activity toward histone H3 lysine 36.

Description:

The FBXL11 Homogeneous Assay Kit is designed to measure the activity of the FBXL11 for
screening and profiling applications. The key to the FBXL11 Homogeneous Assay Kit is a highly specific
antibody that recognizes demethylated substrate. With this kit, only three simple steps on a
microtiter plate are required for demethylase activity detection. First, a sample containing FBXL11
enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody
are added, then donor beads, followed by reading the Alpha-counts.

Green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of culture medium RPMI 1640 at >1% leads to signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.

Format:

Instructions for use:

See product datasheet for detailed instructions.

Storage / Stability:

Stable for at least one year from date of receipt when stored as directed. Be sure to store each component at the proper storage temperature.

Application(s):

Great for studying enzyme kinetics and HTS applications.

Reference(s):

Iwase, S., et al. Cell 2007; 128(6):1077-1088.

Notes:

AlphaScreen® and AlphaLISA® are registered trademarks of PerkinElmer, Inc.

Warning(s):

Avoid freeze thaw cycles.

Scientific Category:

Demethylase

Product Type:

Assay Kit

Data shown is lot-specific. Contact us for specific information on other lots.