Biodegradation of pyrene by Candida sp. S1 under high salinity conditions

Biodegradation of pyrene by Candida sp. S1 under high salinity conditions
Hadibarata, Tony; Khudhair, Ameer; Kristanti, Risky; Kamyab, Hesam
2017-06-13 00:00:00
Polycyclic aromatic hydrocarbon is a toxic recalcitrant environmental pollutant and its removal from the environment is very essential. In this study, a novel S1 strain isolated from the tropical rain forest was identified as Candida species based on 18S rRNA. The pyrene biodegradation was performed by Candida sp. S1. Pyrene was 35% degraded in 15 days. The percentage of pyrene biodegradation increased up to 75% with 24 g L−1 of sodium chloride and decreased along with increasing salinity. Under the acidic condition, the biodegradation was increased up to 60% at pH 5. It was also found that the increasing glucose concentration of more than 10 g L−1 had no significant effect on pyrene biodegradation, while agitation proved to have greater influence. There was a positive relationship between biomass growth and biodegradation rate of pyrene. One pyrene metabolite was identified from the extract solution and analyzed by a thin-layer chromatography, UV–visible absorption and gas chromatography–mass spectrometry. The metabolite found in the pyrene degradation was benzoic acid. Suitable conditions must be found to promote a successful microbial augmentation in liquid culture.
http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.pngBioprocess and Biosystems EngineeringSpringer Journalshttp://www.deepdyve.com/lp/springer-journals/biodegradation-of-pyrene-by-candida-sp-s1-under-high-salinity-gkM3vxBWBU

Biodegradation of pyrene by Candida sp. S1 under high salinity conditions

Recommended Articles

Loading...

References

VOCs and PAHs emissions from creosote-treated wood in a field storage area

Gallego, E; Roca, FJ; Perales, JF; Guardino, X; Berenguer, MJ

New reference value and the background exposure for the PAH metabolites 1-hydroxypyrene and 1- and 2-naphthol in urine of the general population in Germany: basis for validation of human biomonitoring data in environmental medicine