The analysis of seismicity due to the activation of Pernicana Fault in the period 1999-2009 (about 400 earthquakes) has provided useful information for the characterization of this structure. By applying different techniques of location and analysis of cross-correlation on waveforms of earthquakes, it was possible to highlight that the Pernicana Fault is actually composed of segments characterized by different releases of seismic energy. Moreover, the space-time analysis of the evolution of the seismicity showed evident correlation with the eruptive activity of the volcano and the dynamics of its eastern flank.