In recent years, China’s package group tours have a strong consumption capacity, which is conducive to the integration of various industries. In particular, package group tours in leisure agriculture are different from traditional tourism development and package group tours are developing rapidly. Among them, leisure agriculture in the context of collective tourism is conducive to market demand, and on the other hand, it becomes a new type of agricultural production and tourism and tourism because of the needs of rural economic development. However, since the implementation of the “New Tourism Laws” in China in 2013, travel agencies have been banned from arranging shopping trips, which will have an impact on the development of leisure agriculture and rural tourism. Through literature review, this study analyzes the significance of developing leisure agriculture in the context of current package group tours in China. According to the typical case analysis of rural tourism in China, we found the main problems of the new tourism laws in the process of China’s leisure agricultural tourism planning, as a mirror for the future development of leisure agriculture. This study also proposes countermeasures and suggestions for the future development path of China’s leisure agriculture under the new tourism laws.

In a broad sense, agriculture is part of the creative culture industry. Through the concept of verification of the Creative Culture Industry Development Law, this study discusses the core issues that must be paid attention to when promoting the agricultural cultural and creative industries to achieve the lack of economic benefits. This study reviews the definition of cultural and creative industries and the focus of Taiwan’s original culture industry development law and proposes a new concept of an agricultural complex. The concept of an agricultural complex also echoes the urban complex and the tourism complex. Finally, this study proposes conclusions and recommendations for the creative agriculture complex from the two directions of agricultural resource and technology integration.

Abstract:
We modelled the network system by employing a scale-free network of coupled phase oscillators. Pairwise phase coherence (PPC) was calculated for all the pairs of oscillators to present functional dynamics induced by the system. At the regime of global incoherence, we observed a Significant pairwise synchronization only between two nodes that are physically connected. Right after the onset of global synchronization, disconnected nodes begin to oscillate in a correlated fashion and the PPC of two nodes, either connected or disconnected, depends on their degrees.Based on the observation of PPCs, we built a weighted network of synchronization (WNS), an all-to-all functionally connected network where each link is weighted by the PPC of two oscillators at the ends of the link. In the regime of strong coupling, we observed a Significant similarity in the organization of WNSs induced by systems sharing the same substrate network but different configurations of initial phases and intrinsic frequencies of oscillators.We reconstruct physical network from the WNS by choosing the links whose weights are higher than a given threshold. We observed an optimal reconstruction just before the onset of global synchronization.Finally, we correlated the topology of the background network to the observed change of the functional activities in the system.The results presented in this study indicate a strong relationship between the structure and dynamics of complex network systems. As coupling strength increases, synchronization emerges among hub nodes and recruits small-degree nodes. The results show that the onset of global synchronization in the system hinders the reconstruction of an underlying complex structure. Our analysis helps to clarify how the synchronization is achieved in systems of different network topologies.Biological networks, such as protein-protein interactions, metabolic, signalling, transcription-regulatory networks and neural synapses, are representations of large-sc

Abstract:
Necessary requirements for the successful management of an esophageal fistula are thefollowing: functional closure of the defect, control of infection, shortened period of hospitalization,and minimal donor-site morbidity. A sternocleidomastoid (SCM) muscle flap meetsthose requirements. Furthermore, the SCM muscle flap is already a well established treatmentfor non-oncologic patients, although the procedure has received criticism for head andneck reconstruction in patients with malignant disease of the upper aerodigestive tract. Inthis report we describe the utility of a SCM muscle flap, as a buttress for repairing a frankclinical leak after esophageal reconstruction with a free jejunal flap, in a selected patientwith a clinically N0 neck. No clinical leakage was found postoperatively. After a two-year offollow-up, the patient could eat solids without the need for additional nutritional supplementation.No tumor recurrence was noted.

Three coating methods (slot, dip and blade coatings) were used separately to coat a well-dispersed single-wall carbonnanotube (SWCNT) solution on polyethylene terephthalate (PET) film, and the resulting optical and electronic properties were measured and compared. It was found that the sheet resistance and the transparency of the SWCNT coated film decreased as the coating speed increased for dip and blade coatings, but were independent of the coating speed for slot coating. All three coating methods were able to produce transparent conductive film with transparency above 85% and sheet resistance close to 1000 ohm/sq. For industrial production, the slot die coating methodappears to be more suitable in terms of high coating speed and uniformity of optical and electronic properties.

Abstract:
We developed a novel method, the regulatory event-based Gene Set Analysis (eGSA), which considers not only the consistently changed genes but also every gene regulation (event) of each sample to overcome the detection limit. In comparison with conventional methods, eGSA can detect functional changes in heterogeneous samples more precisely and robustly. Furthermore, by utilizing eGSA, we successfully revealed novel functional characteristics and potential mechanisms of very early hepatocellular carcinoma (HCC).Our study creates a novel scheme to directly target the major cellular functional changes in heterogeneous samples. All potential regulatory routines of a functional change can be further analyzed by the regulatory event frequency. We also provide a case study on early HCCs and reveal a novel insight at the initial stage of hepatocarcinogenesis. eGSA therefore accelerates and refines the interpretation of heterogeneous genomic data sets in the absence of gene-phenotype correlations.In the past decade microarray technology has become a popular tool for identifying differentially expressed genes (DEGs) associated with a given phenotype or sample classification. The biological interpretation of transcriptomic changes is commonly revealed by gene function annotation enrichment analysis based on a list of statistically selected DEGs. It is also called as Individual Gene Analysis (IGA) by Nam [1]. However, since only the most significant portion of DEGs was taken into account, the small set of genes might not perfectly represent the whole transcriptomic changes [1-5]. Indeed, Pan et al. showed that the selections of DEGs significantly affected the IGA results [6]. In addition, IGA usually tests functional changes by annotation enrichment methods such as hypergeometric test. These algorithms equally count each DEG so that the significance levels of gene-phenotype correlation are flattened [2].Recently, gene set-based approaches have been proposed to overcome the drawb

Abstract:
In human aortic endothelial cells (HAEC) treated with a series concentrations of AGE-BSA (0-500 μg/ml) for 24 and 48 hours, Cx43 transcript and Cx43 protein were reduced in a dose dependent manner. In addition, gap-junction communication was reduced. To clarify the mechanisms underlying the down-regulation, MAPKs pathways in HAEC were examined. Both a MEK1 inhibitor (PD98059) and a p38 MAPK inhibitor (SB203580) significantly reversed the reductions of Cx43 mRNA and protein induced by AGE-BSA. Consistently, phosphorylation of ERK and p38 MAPK was enhanced in response to exposure to AGE-BSA. However, all reversions of down-regulated Cx43 by inhibitors did not restore the functional gap-junction communication.AGE-BSA down-regulated Cx43 expression in HAEC, mainly through reduced Cx43 transcription, and the process involved activation of ERK and p38 MAPK.Diabetes is known to accelerate the process of atherosclerosis, in which endothelial dysfunction plays a key role [1]. Previous studies have shown that diabetes alters the expression of a variety of molecules involved in maintenance of endothelial function [2]. Animal experiments also demonstrated that induction of diabetes, either alone or on top of hyperlipidemia, suppress the expression of endothelial gap junctions [3,4]. Gap junctions are cell membrane channels made of paired hexamers of connexins, which allow exchange of ions and small signaling molecules between the cytoplasmic compartments of adjacent cells. In mammals endothelial cells mainly express connexin43 (Cx43), Cx40, and Cx37 [5], of which Cx43 is by far the predominant in the cultured endothelial cells [6,7]. Several reports have shown that endothelial Cx43 gap junctions are down-regulated by factors causing endothelial dysfunction [8], such as aging [9], hypertension [10], and arsenic trioxide [11], the last of which was even reported to induce endothelial lesion. In contrast, other factors causing endothelial dysfunction, such as oscillatory shear str

Abstract:
The scale and functional complexity of future-generation wireless sensor networks will call for a non-homogeneous architecture, in which different sensors play different logical roles or functions, or have different physical capabilities in terms of energy, computing power, or network bandwidth. When sensors of the same group need to communicate with each other, their communications often have to pass through other sensors, thus forming an overlay on top of the wireless sensor network. The topology of the overlay is critical. It must have a low diameter to reduce the communication latency between those sensors. It also needs to avoid using other sensors for relaying the communications as much as possible, so as to preserve the energy of other sensors. In this paper, we propose a distributed overlay formation protocol taking account of the above factors. Through simulation, we compare our protocol with two overlay formation protocols, one that generates a fully connected topology and the other a minimum spanning tree. The results show that our protocol can achieve better performance both in message latency and energy consumption.