Fluorescent Secondary Antibodies

fluorescent anti-rabbit & anti-mouse secondary conjugates

Active Motif's fluorescent secondary antibody conjugates provide you with an array of choices for immunofluorescent detection of primary antibodies. Our secondary antibodies are conjugated to a number of high-quality dyes, including the Chromeo™ line of fluorescent dyes. We also offer ATTO dye conjugates that have been maximally cross-adsorbed against IgG's of a variety of species to eliminate background caused by non-specific binding. Chromeo 488, Chromeo 505, Chromeo 494 and the ATTO STED dyes have been certified by Leica Microsystems for STimulated Emission Depletion (STED) microscopy, allowing a spatial resolution of 50 - 70 nm.

Chromeo 488, Chromeo 505, Chromeo 546, ATTO 532 (GSD), ATTO 647N (STED/GSD) and the Rhodamine 6G (GSD) secondary antibody conjugates are recommended by Leica Microsystems for high-resolution GSDIM microscopy, using the novel SR GSD microscope. With this novel high resolution technique a resolution of about 20 nm can be achieved.

Click on antibody conjugate below to see its complete information. To see all of our primary antibodies, please go to the Primary Antibodies page. Or, to find the antibodies you're looking for using a variety of specific search criteria, use the Search box and/or apply the various Filters in the left column.

Chromeo™ Dyes are bright fluorescent labels that replace Alexa Fluor*, DyLight* or Cy*-dyes. They are compatible with most excitation sources including diode lasers, LEDs, tungsten lamps and xenon arc lamps. The dyes have been conjugated to high-quality secondary antibodies by an optimized conjugation method, including subsequent purification from interfering substances.

Fluorescent Chromeo Dyes Secondary Antibody Conjugates enable sensitive and specific detection in fluorescence microscopy, high content screening, ELISA, FRET applications or flow cytometry. To ensure that you get the best results possible, we highly recommend that you use our secondary antibody conjugates together with our MAX Stain™ Immunofluorescence Tools, as those components have been formulated to optimize the performance of the Chromeo™ Dyes.

To receive more detailed information and application data about individual Chromeo dyes, simply click on the name of the Chromeo dye of your choice in the table below. To see a larger spectra image, click on the spectrum of your choice.

Next to the spectral properties of the dye and the quality of the secondary antibody, the quality of a fluorescent conjugate is influenced by the dye-to protein ratio, the conjugation method and its purity. All Active Motif Secondary Antibody Conjugates have been prepared by an optimized conjugation protocol making the fluorescent secondaries brighter and lowering the fluorescent background. Active Motif antibody conjugates have been tested in various applications including flow cytometry and fluorescent microscopy where they have shown to work with high efficiency and specificity under multiple fixation conditions.

Contents & Storage

The Chromeo™ conjugates are supplied as 1 mg of antibody at a concentration of 2 mg/ml. The ATTO and the Rhodamine 6G conjugates are supplied in 200 or 35 µl aliquots in PBS. For short-term storage, the conjugated antibody should be stored at 4°C protected from light. For longer-term storage, aliquot the antibody and store at -20°C. Avoid subjecting the antibody to repeated freeze-thaw cycles. These products are guaranteed for 6 months from the date of arrival.

To ensure photostability of Chromeo 488 under all experimental conditions, one aliquot of MAXfluor™ Mounting Medium for use in fluorescence microscopy is supplied with Chromeo 488 conjugates. This will provide optimal fluorescence stability and anti-fading during long-term storage of your mounted slides. MAXfluor Mounting medium should be stored at 4°C.