Summary: Immunohistochemistry is a method of detecting the presence of specific proteins in cells or tissues and consists of the following steps: 1)primary antibody binds to specific antigen; 2) antibody-antigen complex is bound by a secondary, enzyme-conjugated, antibody; 3) in the presence of substrate and chromogen, the enzyme forms a colored deposit at the sites of antibody-antigen binding.

Bromodeoxyuridine Immunohistochemistry
(Dr. Ornitz's Lab, Washington University Medical School)This method utilizes formalin-fixation, paraffin-embedding, and an anti-BrDU mAb that detects BrDU in ssDNA after denaturation and neutralization. Because it has been optimized for midgestational murine lens analysis, it may require further adjustment for other tissues. http://molecool.wustl.edu/ornitzlab/proto.BrdU.htmAdded: Tue May 14 2002, Hits: 2019, Reviews: 0Write reviewCached

Double Immunohistochemistry
(Giorgio Cattoretti, Columbia University)You should not co-stain in IHC antigens located on the same structure (nucleus, membrane, cytoplasm). That because the success of this double staining procedure is based on the complete development of the first stain, which should mask the structure stained first. Therefore you should be able to use two antibodies raised in the same species. However better results are obtained if two different species are used. http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...Added: Fri Aug 30 2002, Hits: 2557, Reviews: 0Write reviewCached

Immunohistochemistry(Tyner lab)This is a basic immunohistochemistry protocol. Protocols for immunohistochemistry vary widely, due to the differences between antigens and their recognition by antibody. For success it is crucial to try a number of different techniques and compare the results. Added: Tue May 14 2002, Reviews: 0Write review