This antibody is a high quality immunohistochemical reagent that has been used for studies in both the peripheral and central nervous system. It is also useful for double label studies when combined with rabbit or other primary antibodies.

性能

形式

Liquid

存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

靶标

功能

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

组织特异性

Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.

The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.

翻译后修饰

Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).

As for ab72428, I will need to check with the lab regarding tested tissue,localization of staining and potential cross-reactivity with eNOS and iNOS. I will letyou know what I find out. Thank you for your patience. ...

We have 3 antibodies that were tested and are guaranteed (http://www.abcam.com/Abpromise) for IHC-P and rabbit samples: ab1376,ab6175 and ab72428. I would recommend trying one of thosethat were tested as they are...

I'm sorry to hear you are experiencing problems with ab6175, I have received feedback from the laboratory that informed me that your protocol is very similar to ours and we would expect it to work, except the addition of tritonx100 in the antibody dilu...

This anti-NOS antibody has not, to our knowledge, been tested in paraffin sections, but we would be surprised if this antibody did not work in paraffin. It is an antibody that is well suited to immunohistochemistry and we have found it works well for ...