Wolfbane is a dual-bladed silver dagger with magical properties. It has no level requirements to equip. When wielded, the dagger stops the residents of Canifis from morphing into their more powerful Werewolf form. Since Wolfbane prevents the villagers from turning into werewolves, this method does NOT work for Slayer tasks.

The dagger is a reward for completing the Priest in Peril quest. If dropped or lost, the dagger can be reclaimed by talking to Drezel in the basement of the Paterdomus church (there is a ladder north of the church). Multiple daggers can be obtained by using the Drop trick. The dagger has a +5 Prayer bonus, making it quite effective at extending prayer points. Only some expensive, rare weapons have better prayer bonuses.

However, due to its stats being so low, it is not recommended to use this item (unless it is for the prayer bonus) if other options are available.

There is also a plant called wolfsbane, otherwise known as monkshood and aconitum and is traditionally associated with werewolves in various ways.

There is a Wolfbane dagger laying on a workbench in Doric's house. This is a reference to the RuneScape novel, Return to Canifis.

Prior to the Evolution of Combat, the Wolfbane could not be poisoned. A message would appear saying "You cannot poison the holy dagger Wolfbane...". This is no longer the case, due to the changes to weapon poison.