Centrifuge 4 times, extracting the supernatant each time and then redispersing in 250uL 50nM HEPES buffer

Take absorbance of final solution and three supernatants

Absorbance Data

Procedure Update: Silica-coating AuNPs by MHA

We decided to follow the MHA procedure because MHA reduces the number of gold cores per silica-coated particles, and we would ideally have each individual gold nanoparticle covered by a layer of silica. Using the MHA versus not using it has also been very effective in the trials conducted so far. We also think that using MHA will allow us to reach our desired optimal shell thickness of approximately 35nm.