To determine the exact concentration, titrating the hydrochloric acid against a solution of base, for example sodium hydroxide, would be appropriate.

Aliquots of the peptide solution were titrated to the micelle solution to reach a peptide-to-micelle molar ratio of 1: 220.

This solution was titrated with the HSA fragment by adding small amounts of an ~ 0.3 mM protein solution in the aqueous buffer and recording the spectrum after incubation for a few minutes after each addition.

Origin

Derivatives

titratable

The relationship between titratable acidity and malic acid was less obvious.

The sign and magnitude of the pK a shift of a particular titratable group is determined by the free energy to transfer it into the low-dielectric protein cavity, and by its interactions with polar residues in the protein.

The only extra hydrogen bond that is formed involving titratable residues is between OG1 and OE2.