From: "WEAVER,BILL (HP-USA,ex1)" bill.weaver at hp.com> on 2003.09.15 at 15:21:30(10581)A year or two ago there was some discussion on the list as to
whether Amorphophallus titanum could be successfully cloned from a
leaf cutting. So I decided to give it a try. I took two leaflets
off of the big leaf and treated one with Rootone F and one with
Olivia's Cloning Gel. Each leaf was then put in a six inch pot full
of seedling mix (fine vermiculite/sand/perlite) and zipped into a
plastic bag and placed under fluorescent lights. The leaflets stayed
green for almost a year. After they died and rotted I strained the
potting material I found something pretty cool. The Rootone treated
leaflet had produced three tiny bulblets, the largest was about 9mm
and the smallest about 3mm. The Cloning Gel treated leaflet produced
the most interesting growth. The midrib of the leaf had produced two
cylindrical growths, the smaller one was about 2.5cm long and about 7mm
wide.
The other was over 6cm long and 2cm wide. Attached to it were two small
bulblets 5mm and 15mm in diameter. I planted all of the bulblets and
growths in April 2003 and the first new growths sprouted from the
Cloning Gel pot this weekend. To put it mildly I am very happy with the
results and decided to share the story so far.

put it into a light medium in a terrariam.About 2 weeks
after,it had enough roots to support itself in the pot.

From: StroWi at t-online.de (StroWi) on 2003.09.17 at 01:22:00(10587)Bill,

congratulation for your cloning experiment!

I remember very well the discussion on the list on titanum leaf cuttings in summer last year.

Interesting that you got some what different responses depending on the rooting or cloning powder or gel.
Would you have the ingredients or formulation of those products? This would maybe shed a light on the differneces between the two leaf cuttings you grew.

"WEAVER,BILL (HP-USA,ex1)" schrieb:
> A year or two ago there was some discussion on the list as to
> whether Amorphophallus titanum could be successfully cloned from a
> leaf cutting.........
>
> Bill Weaver
> bill.weaver@hp.com
>

From: Douglas Ewing dewing at u.washington.edu> on 2003.09.18 at 08:35:59(10598)I too have been cloning titanum from leaf cuttings. We differ from Bill's
procedure slightly:

I use a 12" portion of the leaf with the mid-vein. I have not
experimented with smaller cuttings.

We apply no hormone. ( this is something I have avoided with all
leaf cuttings in the past, as I was under the impression that auxin would
promote root formation, but that it inhibits formation of shoots. Since we
want both to happen with a leaf cutting, we do not apply. Tubers are
shoots, perhaps this explains the difference in the gel vs. powder
results?)

We stick the cutting into a 8cm cube of rockwool, water, and
encapsulate the entire affair in a terrarium made from 2 2liter
pop-bottles. This creates almost total humidity, and cutting do not wilt.
If/when fungus appears on the cutting, we dust with cinnamon. Tuber
formation
takes several months, then we wean from humidity by utilizing a pop-bottle
with the bottom removed and the cap off, this allows a bit of air exchange
for a few days , then the cutting is removed from the structure, potted up
and placed on the open bench. Typically the orig. leaf tissue dies,
followed by new leaf emergence. We also see multiple leaf emergence, which
I am assuming will result in multiple tubers.

I have not tried other sp. of this genus, but the fact that
several species produce tubercles on the leaf naturally,( A. bulbifer)
makes me think that all sp. might respond to this treatment.

From: "Scott Taylor" staylor at brevardparks.com> on 2003.09.18 at 14:01:32(10599)Just so I am clear on this cloning technique: does one use the entire leaf,
or trim the lateral material away from the midvein? And how much of the
petiole is retained?

I too have been cloning titanum from leaf cuttings. We differ from Bill's
procedure slightly:

I use a 12" portion of the leaf with the mid-vein. I have not
experimented with smaller cuttings.

We apply no hormone. ( this is something I have avoided with all
leaf cuttings in the past, as I was under the impression that auxin would
promote root formation, but that it inhibits formation of shoots. Since we
want both to happen with a leaf cutting, we do not apply. Tubers are
shoots, perhaps this explains the difference in the gel vs. powder
results?)

We stick the cutting into a 8cm cube of rockwool, water, and
encapsulate the entire affair in a terrarium made from 2 2liter
pop-bottles. This creates almost total humidity, and cutting do not wilt.
If/when fungus appears on the cutting, we dust with cinnamon. Tuber
formation
takes several months, then we wean from humidity by utilizing a pop-bottle
with the bottom removed and the cap off, this allows a bit of air exchange
for a few days , then the cutting is removed from the structure, potted up
and placed on the open bench. Typically the orig. leaf tissue dies,
followed by new leaf emergence. We also see multiple leaf emergence, which
I am assuming will result in multiple tubers.

I have not tried other sp. of this genus, but the fact that
several species produce tubercles on the leaf naturally,( A. bulbifer)
makes me think that all sp. might respond to this treatment.

From: "WEAVER,BILL (HP-USA,ex1)" bill.weaver at hp.com> on 2003.09.19 at 15:02:31(10608)I used just a leaflet one of the little (4" X 8") oval components that make
up the entire leaf. I just took one that came to a nice point with very
little
leaf tissue on either side of the mid-rib where I cut it off.

I too have been cloning titanum from leaf cuttings. We differ from Bill's
procedure slightly:

I use a 12" portion of the leaf with the mid-vein. I have not
experimented with smaller cuttings.

We apply no hormone. ( this is something I have avoided with all
leaf cuttings in the past, as I was under the impression that auxin would
promote root formation, but that it inhibits formation of shoots. Since we
want both to happen with a leaf cutting, we do not apply. Tubers are
shoots, perhaps this explains the difference in the gel vs. powder
results?)

We stick the cutting into a 8cm cube of rockwool, water, and
encapsulate the entire affair in a terrarium made from 2 2liter
pop-bottles. This creates almost total humidity, and cutting do not wilt.
If/when fungus appears on the cutting, we dust with cinnamon. Tuber
formation
takes several months, then we wean from humidity by utilizing a pop-bottle
with the bottom removed and the cap off, this allows a bit of air exchange
for a few days , then the cutting is removed from the structure, potted up
and placed on the open bench. Typically the orig. leaf tissue dies,
followed by new leaf emergence. We also see multiple leaf emergence, which
I am assuming will result in multiple tubers.

I have not tried other sp. of this genus, but the fact that
several species produce tubercles on the leaf naturally,( A. bulbifer)
makes me think that all sp. might respond to this treatment.

> What was the timeframe before the new shoots appeared? I had to dig them up
> and repot them before anything happened.
>
> Bill
>
> -----Original Message-----
> From: Douglas Ewing [mailto:dewing@u.washington.edu]
> Sent: Thursday, September 18, 2003 8:36 AM
> To: 'aroid-l posting'
> Subject: Re: [aroid-l] A. Titanum cloned
>
>
> I too have been cloning titanum from leaf cuttings. We differ from Bill's
> procedure slightly:
>
> I use a 12" portion of the leaf with the mid-vein. I have not
> experimented with smaller cuttings.
>
> We apply no hormone. ( this is something I have avoided with all
> leaf cuttings in the past, as I was under the impression that auxin would
> promote root formation, but that it inhibits formation of shoots. Since we
> want both to happen with a leaf cutting, we do not apply. Tubers are
> shoots, perhaps this explains the difference in the gel vs. powder
> results?)
>
> We stick the cutting into a 8cm cube of rockwool, water, and
> encapsulate the entire affair in a terrarium made from 2 2liter
> pop-bottles. This creates almost total humidity, and cutting do not wilt.
> If/when fungus appears on the cutting, we dust with cinnamon. Tuber
> formation
> takes several months, then we wean from humidity by utilizing a pop-bottle
> with the bottom removed and the cap off, this allows a bit of air exchange
> for a few days , then the cutting is removed from the structure, potted up
> and placed on the open bench. Typically the orig. leaf tissue dies,
> followed by new leaf emergence. We also see multiple leaf emergence, which
> I am assuming will result in multiple tubers.
>
> I have not tried other sp. of this genus, but the fact that
> several species produce tubercles on the leaf naturally,( A. bulbifer)
> makes me think that all sp. might respond to this treatment.
>
>
> Doug
> _________________/\/\/\______________________
>
> Doug Ewing, Greenhouse Manager (206) 543-0436
> Department of Biology
> University of Washington
> Box 351800
> Seattle, WA 98195-1800
>
>
>
>
>