Osteocalcin Detection

Osteocalcin, also known as bone gamma-carboxyglutamate protein, is a vitamin K- and D-dependent, calcium-binding, non-collagenous protein. It has a molecular weight of approximately 5.9 kDa and contains 2–3 gamma-carboxyglutamate residues (Gla). Osteocalcin is produced only by osteoblasts and their dental counterpart, odontoblasts, and is an indicator of bone metabolism. The carboxylated form of osteocalcin is an indicator of bone formation, while its undercarboxylated counterpart is involved in glucose metabolism. Osteocalcin also acts as a hormone that regulates glucose and fat deposition as well as male fertility.

ELISA Kits for Osteocalcin Detection

The Human Gla-Osteocalcin High Sensitive EIA Kit is designed to specifically differentiate human from bovine osteocalcin using a human osteocalcin-specific coated plate. This coated plate contains a monoclonal antibody that distinguishes human from bovine osteocalcin via their amino acid differences at positions 3 and 4 in the N-terminus. Using a human antigen-specific monoclonal antibody for capture greatly improves experimental linearity with human blood samples.

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.