[Histonet] Mayer's Hematoxylin

From:

"Bauer, Karen"

Hello to all,
I'm looking for a Pre-Made Mayer's Hematoxylin that we can use for our routine H&E staining procedure. We make up our Mayer's Hematoxylin from scratch, but have recently been given permission to buy something that is pre-made. We've tried Harris, but the doctors do not like the differentiation that take place with using that kind of hematoxylin. (We stain by hand, so one tech's dip might be longer or shorter than another tech's dip in the differentiator.) They are seeing variations in nuclear staining, no matter how careful we are with our "dips".
I'm looking for something that stains tissues in 5-10 minutes and does not need differentiation. Everything that I've tried so far is not working out to their satisfaction. We use a Gill's III for our FS set up, and they do not want that either (for the routine H&E's).
Does anyone have any ideas or tips for me? I'll be greatly appreciative.
Thanks in advance,
Karen Bauer HT(ASCP)
Histology Supervisor
Luther Hospital
Eau Claire, WI
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