Biolayer interferometry measurement of biological targets

Disclosed are methods and compositions for the ultrasensitive detection of oligonucleotides,
proteins, protein complexes, biomolecules, and infectious agents using a peroxidase
driven deposition of substrates onto interferometry capable biosensors, coupled to
the specific recognition of the target molecules. More specifically, methods are disclosed
to specifically immobilize biological target molecules onto the surface of interferometry
capable biosensors and to associate the target molecules with peroxidase enzymes.
Through the peroxidase driven deposition of substrates onto the interferometry capable
biosensors there is the ability to achieve ultrasensitive detection and quantification
of specific target molecules.