RE: Artefact on IHC slides

From:

"Dawson, Glen"

Laura,
My guess is that it is an adhesion problem. Maybe the slide that the tissue
was put on had a piece of gauze or something of the like sitting on it that
rubbed away the adhesive properties of the slide in a definite pattern. In
the spots where the adhesion was gone, the tissue lifted trapping DAB under
the tissue.
Glen Dawson.
-----Original Message-----
From: bliven.laura@marshfieldclinic.org
[mailto:bliven.laura@marshfieldclinic.org]
Sent: Wednesday, October 30, 2002 1:26 PM
To: histonet@pathology.swmed.edu
Subject: Artefact on IHC slides
Please view pictures on:
histonet.org and click on View List Serv Images
to view ipproblem1.jpg and ipproblem2.jpg.
The stain is IHC CD20 on a biopsy specimen using DAB as the chromogen and
Hematoxylin as the counterstain.
As far as I know, the tissue didn't come in contact with gauze and the
artifact pattern isn't consistent with our tissue cassettes. Just curious
as to any/all answers on this one.
Thanks,
Laura Bliven
Marshfield Laboratories
bliven.laura@marshfieldclinic.org