Hi.
I'm doing PCR for site specific mutagenesis and the
region of the gene we are trying to mutagenise is GC rich in the
area that encodes for the amino acid residue. I was wondering
if...
1. Someone can input my two primers into an OLIGO program to see
if they will dimerise....
primer 1 -> 5' GATCAGXCTATGTGGCGGATGAGCG 3'
T
A
primer 2 -> 3' ACCTCACTGGCCGTCAATAGACCTT 5'
2. Someone (it can be the same person) can check if the primers
will fold up on themselves (ie. secondary structure....hairpins
etc)
3. If this doesn't work, then I was wondering if anyone can tell
me where to get some more information (ie. shareware I may able
to obtain...)
4. As an aside, I did PCR with these primers but was unable to
get any product. The first time, though, I did get a TINY TINY
amount but not enough to do a proper transformation with.
YOUR ANTICIPATED HELP IS VERY APPRECIATED. (I've had many
sleepless nights for the past few months!)
ThankyouhankyouThankyouThankyouThankyouThankyou
Sincerely,
Calvino Cheng