G Protein Coupled Receptors: Structure by P. Michael Conn (Eds.)

By P. Michael Conn (Eds.)

This new quantity of Methods in Enzymology maintains the legacy of this optimum serial through containing caliber chapters authored via leaders within the box. This quantity covers G protein coupled receptors and comprises chapters on such issues as post-translation amendment of GPCR in dating to biased agonism, structure-based digital screening, and GPCR oligomerization within the brain.

Continues the legacy of this most excellent serial with caliber chapters authored by means of leaders within the field

Covers G protein coupled receptors

Contains chapters on such subject matters as post-translation amendment of GPCR in courting to biased agonism, structure-based digital screening, and GPCR oligomerization within the brain

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Tip: Add 1 mL of 100 Â PMSF stock directly to SDS-PAGE sample buffer aliquot before adding membranes. Detergent solubilization of membranes typically sets free protease activities that continue to function robustly in SDS-PAGE sample buffer prior to electrophoresis. Also, do not subject GPCR membrane/protein samples to heating/boiling SDS-PAGE sample buffer as membrane proteins typically aggregate upon heating. 3. , 2006). Although optimization of various parameters that affect protein production is very labor and time intensive, making the effort to do this has, thus far for 20 GPCRs, always resulted in a major improvement in production level of functional receptor.

Receptors in the left group are ordered (left to right) by decreasing an improvement factor for the combined treatment which resulted in the greatest improvement. Receptors in the right group are ordered (left to right) by decreasing an improvement factor where individual factors have the greatest effect. Species: H, human; M, mouse; P, pig; R, rat. Receptor UniProt IDs left to right: P41145, P14416, P33534, P18089, P06199, P47898, P08172, P25103, P16610, P25929, P11229, P28222, P25021, P61168, P35368, P12657, P21452, P28221, P29371, and P29274.