Antibody Cross-Reactivity

Wax-it Histology Services provides tissue cross-reactivity services, allowing researchers to investigate and validate that an antibody of interest does not bind to epitopes other than the expected target site. Identification of antibody cross-reactivity in the discovery / pre-clinical stage of drug development can help to avoid adverse and unforeseen toxicological effects in future human clinical trials.

Wax-it Histology Services utilizes an in-house TissueMacroArray system to permit rapid screening of potential tissue cross-reactivity. These TissueMacroArrays incorporate multiple arrayed tissues from the same species into a single tissue block, providing more consistent, reliable immunohistochemical staining, and significantly reduced costs (as multiple tissues are stained on a single slide).

A comprehensive antibody cross-reactivity program is offered to support our clients’ research needs:

Complete tissue banks of human, mouse, rat, and pig

Full medical history of human tissues

Study-specific protocols that reflect the sponsor’s requirements

Unparalleled expertise in immunohistochemistry, histology, immunopathology, and protocol development

Tissue analysis conducted by board-certified pathologists

Comprehensive, high-quality reports

Digital photomicroscopy and/or digital whole-slide scanning

Rapid turn-around times

Cost-effective pricing

* TissueMacroArrays are available for human tissue upon request

Our Process

We first determine the optimal specificity and staining conditions for the experimental antibody. Multiple target retrieval, antibody dilutions, and appropriate positive and negative controls are used to optimize and validate immunostaining conditions.

Based on this optimized protocol, we will perform immunostaining across 32 standard tissues, followed by evaluation and report generation. Staining of tissues can be done on an individual basis (i.e. – one tissue per slide), or by incorporating our TissueMacroArray bank into the study.

Adrenal Gland

Endothelium

Ovary

Spinal Cord

Bladder

Eye

Pancreas

Spleen

Blood Cells

Fallopian Tube

Parathyroid

Testes

Bone Marrow

Gastrointestinal Tract

Pituitary

Thymus

Breast

Heart

Placenta

Thyroid

Cerebellum

Kidney

Prostate

Ureter

Cerebral Cortex

Liver

Skeletal Muscle

Uterus

Colon

Lymph Node

Skin

Entire data sets can be collected via digital whole-slide scanning (particularly useful when combined with our TissueMacroArrays), with all stained information being analyzed by a board-certified pathologist and corresponding report generated detailing the findings.