Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Specificity and Use

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Murine IL-6 by direct ELISA (using 100 ml/well) a concentration of approximately 1 ug/ml of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Murine IL-6. To detect Murine IL-6 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine IL-6 (LS-C104805) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine IL-6. Western Blot: To detect Murine IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-6 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Presentation:

Lyophilized from PBS, pH 7.2

Reconstitution:

Sterile PBS containing 0.1% BSA

Storage:

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Description: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine.