If coverslip is preferred, allow slides to air-dry; dip dried slides in xylene and coverslip with compatible mounting medium.

RESULTS:

Erythrocytes:

Pink to yellowish-red

Platelets:

Violet or purple granules

Granulocytes

Neutrophils:

Nucleus - Dark blue to violet

Cytoplasm - Pale pink

Granules - Purple to lilac

Eosinophils:

Nucleus - Blue

Cytoplasm - Blue

Granules - Red to red-orange

Basophils:

Nucleus - Purple or dark blue

Granules - Dark purple

Mononuclear Cells

Monocytes:

Nucleus - Violet

Cytoplasm - Sky blue

Lymphocytes:

Nucleus - Violet

Cytoplasm - Dark blue

Bacteria/microorganisms:

Deep blue in varying shapes

Muscle and collagen:

Pale Pink

Nuclei:

Blue/violet

Cytoplasm:

Varying shades of light blue

DIFFERENTIAL STAIN KIT PROCEDURE NOTES FOR SMEARS & TOUCH IMPRINTS:

The division of stains in this kit gives the user the advantage of varying dips in Solutions A and B to produce different degrees of shading and intensity. However; never use fewer than three dips of one full second each.

If more intense overall stain is desired, increase the number of dips in Solutions A and B.

To increase eosinophilic staining; increase the number of dips in Solution A.

To increase basophilic staining; increase the number of dips in Solution B.

If a paler stain is desired; decrease dips in Solutions A and B.

If using a xylene substitute, closely follow the manufacturer’s recommendations for coverslipping application.