Transcriptomic analysis of Bama pig's liver in various nutritional states reveals a metabolic difference of fatty acids

a
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, P.R. China
E-mail:zhoulei@gxu.edu.cnTel: +86-771-3231158

Abstract

Both fasting and treatment with a high-fat diet (HFD) can dramatically change fat metabolism in the liver and, thus, are commonly used methods to investigate hepatic fat metabolism and related diseases. Here, the gene expression profiles of pig liver under both conditions were investigated and changes in hepatic triacylglycerol (TG) levels under different diet conditions were determined. In this study, both fasting and HFD conditions significantly increased hepatic TG levels and serum levels of cholesterol, TG, high-density lipoprotein, low-density lipoprotein, and apolipoprotein B. Transcriptome sequencing analysis identified 580 differentially expressed genes (DEGs) between the fasting group and the control group (F/C group) and 613 between the HFD group and the control group (H/C group). Kyoto Encyclopedia of Genes and Genomes enrichment analysis found that the DEGs of the F/C group were mainly enriched in the synthesis pathways of fatty acids (FAs) with less than 16 carbons, while the DEGs of the H/C group were mainly enriched in the synthesis pathways of FAs with more than 16 carbons. In order to verify whether changes in the expression levels of the DEGs caused changes in FA metabolism, the composition and saturation of the FAs in liver TG were analyzed, which showed that under fasting conditions, the contents of monounsaturated and polyunsaturated FAs had increased, while the proportion of saturated FAs had decreased. However, the content of polyunsaturated FAs decreased, while the contents of monounsaturated and saturated FAs increased under HFD conditions. These results will help clarify the differences in FA metabolism in the liver under different nutritional states and indicate that the proportion of unsaturated FAs had increased in hepatic fat under fasting conditions.

Authors contributing to RSC publications (journal articles, books or book chapters)
do not need to formally request permission to reproduce material contained in this
article provided that the correct acknowledgement is given with the reproduced material.

Reproduced material should be attributed as follows:

For reproduction of material from NJC:
Reproduced from Ref. XX with permission from the Centre National de la Recherche
Scientifique (CNRS) and The Royal Society of Chemistry.

For reproduction of material from PCCP:
Reproduced from Ref. XX with permission from the PCCP Owner Societies.

For reproduction of material from PPS:
Reproduced from Ref. XX with permission from the European Society for Photobiology,
the European Photochemistry Association, and The Royal Society of Chemistry.

For reproduction of material from all other RSC journals and books:
Reproduced from Ref. XX with permission from The Royal Society of Chemistry.

If the material has been adapted instead of reproduced from the original RSC publication
"Reproduced from" can be substituted with "Adapted from".

In all cases the Ref. XX is the XXth reference in the list of references.

If you are the author of this article you do not need to formally request permission
to reproduce figures, diagrams etc. contained in this article in third party publications
or in a thesis or dissertation provided that the correct acknowledgement is given
with the reproduced material.

Reproduced material should be attributed as follows:

For reproduction of material from NJC:
[Original citation] - Reproduced by permission of The Royal Society of Chemistry (RSC) on behalf of the
Centre National de la Recherche Scientifique (CNRS) and the RSC

For reproduction of material from PCCP:
[Original citation] - Reproduced by permission of the PCCP Owner Societies

For reproduction of material from PPS:
[Original citation] - Reproduced by permission of The Royal Society of Chemistry (RSC) on behalf of the
European Society for Photobiology, the European Photochemistry Association, and
RSC

For reproduction of material from all other RSC journals:
[Original citation] - Reproduced by permission of The Royal Society of Chemistry

If you are the author of this article you still need to obtain permission to reproduce
the whole article in a third party publication with the exception of reproduction
of the whole article in a thesis or dissertation.

Information about reproducing material from RSC articles with different licences
is available on our Permission Requests page.