CMC of SDS varies with pH. It is about 0.01 mol/L (M) for pH 5-10. At more acidic pH the CMC drops off (Rahman Brown '83 [http://www3.interscience.wiley.com/cgi-bin/fulltext/104026844/PDFSTART]).

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CMC of SDS varies with pH. It is about 0.01 mol/L (M) for pH 5-10. At more acidic pH the CMC drops off to about 0.07M at pH 2 (Rahman Brown '83 [http://www3.interscience.wiley.com/cgi-bin/fulltext/104026844/PDFSTART]).

== SDS removal ==

== SDS removal ==

Use an ion retardation columns to remove SDS from protein samples.

Use an ion retardation columns to remove SDS from protein samples.

Retardation Resin. For SDS removal methods see Kapp'78 (PMID 9762103) and similar papers [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=link&linkname=pubmed_pubmed_reviews&uid=9762103&ordinalpos=1&log$=relatedarticles&logdbfrom=pubmed]. Methylene blue can be used to determine the remaining amount of SDS.

Retardation Resin. For SDS removal methods see Kapp'78 (PMID 9762103) and similar papers [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=link&linkname=pubmed_pubmed_reviews&uid=9762103&ordinalpos=1&log$=relatedarticles&logdbfrom=pubmed]. Methylene blue can be used to determine the remaining amount of SDS.

Revision as of 10:55, 8 April 2009

SDS (sodium dodecyl sulfate/sulphate) is an anionic detergent effective in both acidic and alkaline solutions. SDS has a wide variety of applications, but is most often used in protein and lipid solubilisation.

SDS solubilisation

As a rule of thumb for the solubilisation/solubilization of proteins, SDS should be used at its critical micelle concentration (Womack'83 PMID 6882760). For the effects of SDS on protein conformation see Protein Structure by Creighton [1]. For comparisons between SDS and other detergents for solubilisation of lipids, proteins, and its effect on
enzymes activity see Womack '83 (PMID 6882760) and Creighton Protein Structure.

Critical micelle concentration CMC of SDS

CMC of SDS varies with pH. It is about 0.01 mol/L (M) for pH 5-10. At more acidic pH the CMC drops off to about 0.07M at pH 2 (Rahman Brown '83 [2]).

SDS removal

Use an ion retardation columns to remove SDS from protein samples.
Retardation Resin. For SDS removal methods see Kapp'78 (PMID 9762103) and similar papers [3]. Methylene blue can be used to determine the remaining amount of SDS.