In 1506, the vault was visited by Ezio Auditore and Leonardo da Vinci during their exploration of the Pythagorean temple. In it they were confronted by a series of number and letter codes: "43 39 19 N, 75 27 42 W". Though Leonardo thought that the sequence meant nothing, Ezio simply remarked that it was not meant for either of them.

In 1527, Giovanni Borgia and Maria Amiel visited the vault. There, Giovanni suffered a type of transformation, and an entity by the name of Consus spoke through him.