ABSTRACT: This research aimed
to know the ability of C. hystrix to protect unsaturated fatty acid on the hydrogenation
process by rumen microbes. Cooking oil was tested to determine the fat
profile,then mixed with skim milk (1:2). The mixture were added by C. hystrix
leaves powder with the level of 0%, 1%, 2%, 3% and 4% (dry weight bases) from
the weight of the mixture, then mixed evenly to form protectedcooking oil. The
oil was added into a syringe, containing 300 mg of feed substrates, elephant
grass andbran= 60:40, then added with 30 ml of rumen fluid and buffer mixture.
The syringe was incubated at 39oCfor 48 hours. The data was analyzed by
completely random design. The difference of means the treatments were tested by
Duncan’s new multiple range test. Result showed that oleic and linoleic contentresulted
from fermentation of cooking oil protected by C. hystrix leaves powder was
increase compared to unprotected. It can be concluded that cooking oil
protected with C. hystrix leaves powder reduced hydrogenation of unsaturated
fatty acid, mainly oleic and linoleic.