Intended Use

Principle of the Assay

The RIAKEY Free T4 RIA tube II is based on a competitive radioimmunoassay (RIA). During the incubation, the sample/calibrator T4 competes with the T4 labeled with Iodine-125 (conjugate) for the specific sites of the anti-serum coated on the tubes. B/F separation is based on the use of antibody coated tubes, where the anti-T4 antiserum is fixed on the tube walls. After aspiration, the radioactivity in the tubes is measured in a gamma counter. The degree of binding will be inversely proportional to the sample/calibrator hormone concentration.

Do not use mixed reagents from different lots.

Do not use reagents beyond the expiration date.

Use distilled water stored in clean container.

Use an individual disposable tip for each sample and reagent, to prevent the possible cross-contamination among the samples.

Store the unused coated tubes at 2~8ºC in the appropriate bags with silica gel and accurately sealed.

If large quantity of assay would be performed at one time, there might be substantial time variation between 60 tubes at one time to minimize time variation. Also, do not exceed 10 minutes for entire pipetting.