Each level is split up into two parts, plus a bonus level if the player finishes with at least 125 stamps. Players get a choice between playing as either:

Wile E. Coyote - Players get to use his tricks which he uses in the Looney Tunes cartoons. By jumping into an ACME box players can fly in his green flyer suit, skate, get springs on his feet to jump and so on. Catching up to the Road Runner earns the player various benefits though this is not required to complete the level. Playing as Wile E. Coyote puts a heavier emphasis on platforming and avoiding traps.

Road Runner - Players must try to escape from Wile E. Coyote and complete the level by getting to the other end. If players get caught by Wile E. Coyote, they lose health. Road Runner can scare Wile E. Coyote by using his "meep meep" catchphrase or running him over, which gives the player items. Playing as the Road Runner focuses on gaining speed with springs, speed wheels, and looping tracks.