Specifically, the scientists found that the DNA methylation and gene expression patterns in nuclear transfer ES cells more closely resembled those of ES cells than did iPS cells, which revealed alterations apparently caused by the reprogramming process itself.

has made it known that he believes Missouri should take the lead in outlawing somatic cell nuclear transfer, regardless of what good may come of the research or the jobs or money the effort brings to the state.

Examples of research topics that could be addressed under this program announcement are 1) strategies for culturing pluripotent rat ESCs to allow genetic manipulation and to create rats with germ-line transmission of genetic modifications, 2) development of alternative technologies to create null mutations or gene replacement in the rat, 3) development of cost-effective nuclear transfer procedures in the rat, 4) studies that demonstrate mutation transfer to rat stem cells or other cells for transfer into embryos or germ cells, and 5) methods for targeting engineered introns into rat chromosomal DNA to support the study of gene function.

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