ABSTRACT:A novel cross linker for hydrogel fabrication was synthesized starting from D-glucose. The synthesis was achieved in six steps with good yield. Polyacrylamide-grafted-polymethacrylated glucose (PAM-g-PMAGlc) hydrogels were synthesized by free radical initiated graft copolymerization of acryl amide (AAm) onto poly(methacrylated glucose) (PMAGlc) using commercially available N,N’-Methylenebisacrylamide (MBA) and the synthesized bis-methacrylate as cross linker. A comparative study of the hydrogels obtained from two cross linkers was done. The hydrogels were characterized by Fourier Transform Infrared (FTIR) spectrum, thermal study was investigated using thermogravimetric analysis and DSC while morphology of the gels was examined using Scanning electron microscope (SEM) analysis.