Technology

The iTOP intracellular delivery technology is based on a combination of small molecule compounds which forces the uptake of large gulps of extracellular fluid (containing the bioactive molecules) by the cell (D’Astolfo et al. 2015, Cell 161: 674-690).

Once inside, the vesicles release their content into the cytoplasm, where the bioactive molecules can exert their therapeutic action.

The iTOP technology efficiently transduces native proteins, peptides, nanobodies and small oligonucleotides into virtually any cell type and can be fully optimized for specific biologicals or target cell types.