Located in northwest North Carolina's Yadkin Valley wine country, Winston-Salem is the largest hyphenated city in the United States and the worlds leading regenerative medicine research center. The city is also a leader in health care and has two of the three largest hospitals in the state, including one of the largest university medical centers in the southeast United States. Winston-Salem hosts several largest festivals each year. Among them are the state beer festival, Bookmarks, RibFest, the state wine festival and a regional wine festival downtown, along with the River Run International Film Festival and the National Black Theater Festival. Winston-Salem is known as The City of the Arts for its many arts attractions and firsts in the arts. Its also the best preserved colonial city in the nation, with several downtown buildings dating back to the mid-1700s.