Jacqueline Vanderstappen (jacqueline.vanderstap at agr.kuleuven.ac.be) wrote:
: Hello,
: I have some questions concerning the use of the automated ABI 373 stretch
: sequencer.
: We've recently bought the upgrade version (34cm WTR). Sometimes we have
: problems with directly sequencing dsPCR products with the Taq dye terminator
: kit. It's not a problem of reactions because the sequencing with the same
: reactions have worked with the previous version of ABI 373 (24cm WTR). Is
: this a problem of the gel (other concentration) or/and of the running
: conditions. We now run the samples at 30 W.
: Is this too high for PCR products?
: We tried to use the Sequenase dye terminator kit for dsPCR products but it
: didn't work? Is there somebody who has good experience with it? We perform
: the sequencing directly on double-stranded PCR products.
: Thanks,
: Vander Stappen Jacqueline
: KULeuven
: Laboratory of Gene Technology
: W.deCroylaan 42
: 3001 Heverlee
: Belgium
: e-mail: Jacqueline.Vanderstap at agr.kuleuven.ac.be
:Try contacting Petro Bosch of ABI Perkin Elmer Nederland his phone is
01820/75481
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Laboratory of Farmaceutical Biotechnology