Contributions

It is vitally necessary to measure the cellular temperature to fully understand life sciences. A method allowing to measure the cellular temperature for a normal growing state without doing damage to the cells and disturbing their intercellular communication is needed. Here, a wireless, real-time, high-throughput temperature detection method is developed. The acquisition system applies a high-precision reference resistor and a low real-time measurement current (below or equal to 0.14 mA) to reduce self-heating via the intermittent measurement. Cells of a small volume cell medium are cultured on the surface of the platinum thermal resistor and subsequently measured in the incubator.