Heat shock proteins, HSPís or stress response proteins SRPís, are synthesized in a variety of environmental and patholphysiological stressful conditions. Many HSPís are involved in processes such as protein denaturation-renaturation, folding-unfolding, transport-translocation, activation-inactivation, and secretion. HSP70 is found to be associated with steroid receptors, actin, p53, polyoma T antigen, nucleotides, and other unknown proteins. Also, HSP70 has been shown to be involved in protective roles against thermal stress, cytotoxic drugs, and other damaging conditions.

Catalog #

H1830-94N

Applications

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution

Immunohistochemistry (Paraffin/frozen): 1:75-1:250 in an ABC all method. Incubate for 30 minutes at RT.

Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Clone Type

Polyclonal

Isotype

IgG

Host

Rabbit

Source

Human

Concentration

Not Determined

Form

Supplied as a liquid, sodium azide.

Purity

Serum

Immunogen

Purified heat shock protein (Dnak) from E. coli.

Cellular Localization

Cytoplasmic, nuclear.

Specificity

Recognizes the two major human HSP 70s (HSP 72 and HSP 73). Stains epithelial cells in many different tissues (e.g. tonsil, stomach, small and large intestine, bile ducts, ovary and prostate).

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.