4840820170327111930.0doi10.1155/2014/328348sideral94352ART-2014-94352engLuesma MJUniversidad de Zaragoza(orcid)0000-0003-4071-1467New insights into c-Ret signalling pathway in the enteric nervous system and its relationship with ALS.2014The receptor tyrosine kinase Ret (c-Ret) transduces the glial cell line-derived neurotrophic factor (GDNF) signal, one of the neurotrophic factors related to the degeneration process or the regeneration activity of motor neurons in amyotrophic lateral sclerosis (ALS). The phosphorylation of several tyrosine residues of c-Ret seems to be altered in ALS. c-Ret is expressed in motor neurons and in the enteric nervous system (ENS) during the embryonic period. The characteristics of the ENS allow using it as model for central nervous system (CNS) study and being potentially useful for the research of human neurological diseases such as ALS. The aim of the present study was to investigate the cellular localization and quantitative evaluation of marker c-Ret in the adult human gut. To assess the nature of c-Ret positive cells, we performed colocalization with specific markers of cells that typically are located in the enteric ganglia. The colocalization of PGP9.5 and c-Ret was preferentially intense in enteric neurons with oval morphology and mostly peripherally localized in the ganglion, so we concluded that the c-Ret receptor is expressed by a specific subtype of enteric neurons in the mature human ENS of the gut. The functional significance of these c-Ret positive neurons is discussed.Access copy available to the general publicUnrestrictedinfo:eu-repo/semantics/openAccessby-nc-ndhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/1.5792014MEDICINE, RESEARCH & EXPERIMENTAL85 / 123 = 0.6912014Q3T3BIOTECHNOLOGY & APPLIED MICROBIOLOGY106 / 162 = 0.6542014Q3T2info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionCantarero IÁlvarez-Dotu JMUniversidad de Zaragoza(orcid)0000-0002-5106-4843Santander SUniversidad de Zaragoza(orcid)0000-0002-1275-2600Junquera C.Universidad de Zaragoza(orcid)0000-0002-9951-10751004275Universidad de ZaragozaDepartamento de Cirugía, Ginecología y ObstetriciaEstomatología1003443Universidad de ZaragozaDepartamento de Anatomía e Histología HumanasHistología1005315Universidad de ZaragozaDepartamento de Farmacología y FisiologíaFarmacología1003027Universidad de ZaragozaDepartamento de Anatomía e Histología HumanasAnatomía y Embriología Humana2014 (2014), 328348 [7 p.]BioMed res. int.BioMed Research International2314-61334261414http://zaguan.unizar.es/record/48408/files/texto_completo.pdfVersión publicada100503http://zaguan.unizar.es/record/48408/files/texto_completo.jpg?subformat=iconiconVersión publicadaoai:zaguan.unizar.es:48408articulosdriver2016-04-14-11:50:25ARTICLE