This kit is for automated large scale synthesis of a target protein using the patented SECF(Stepwise Exchange Cell-Free) protein synthesis technology. After adding template DNA into ExiProgen™ and choosing protocol No. 903, up to 500 µg of target protein can be automatically synthesized within a day. All processes including protein synthesis and dialysis will be performed in an automated fashion and the final protein harvested in a storage solution of choice.

Features and Benefits

Fully automatic: DNA-in-Protein (in storage buffer)-out
By simply installing 1-10 µg of template DNA into ExiProgen™, the target protein is synthesized and harvested in
any storage buffer of choice.

Parallel processing
1-8 different proteins can be synthesized in a single run.

High purity and high yield
Up to 500 ug of protein can be obtained per well with a purity of >90%.

Procedure and principle

This kit is designed for automated large scale expression of a target protein using patented SECF technology using ExiProgen™. It utilizes cell-free protein synthesis and purification using Ni-NTA magnetic beads. As it provides energy source in a stepwise manner into reaction tubes, the yields are maximized.

As described above, template DNA is mixed in a reaction tube with E. coli extract and Master mix. The reaction tube is installed into ExiProgen™' s reaction block along with other reagents. Finally, choose protocol and initiate run.

ExiProgen™ begins to synthesize the target protein by providing the energy source in stepwise manner. The synthesized target protein is then purified via Ni-NTA magnetic beads.

Experimental results

1. Up to eight proteins with various sizes can be synthesized in a single run.