Matrices listed below were spiked with certain level of TrkA/NTRK1 and the recovery rates were calculated by comparing the measured value to the expected amount of TrkA/NTRK1 in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

94-98

96

EDTA plasma(n=5)

91-95

93

heparin plasma(n=5)

90-102

95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TrkA/NTRK1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.