Has anyone out there in Histoland ever heard (or perhaps done this) of
placing tissue in 70% ethanol, and then freezing to -80 degrees C? I was
speaking to a researcher today, who has some tissue (bone with soft tissue
I believe) samples stored in this manner.
My first fear is artifact from the freezing/thawing process.
How does this storage effect staining, both special and IHC?
Thank you
Patrick M. Haley B.S. HTL(ASCP)
Chief Technologist
HistoTechNologies, inc.
www.histology.net