For the Senate, 237 single-seat constituencies were established, even if the assembly had risen to 315 members. The candidates needed a landslide victory of two thirds of votes to be elected, a goal which could be reached only by the German minorities in South Tirol. All remained votes and seats were grouped in party lists and regional constituencies, where a D'Hondt method was used: inside the lists, candidates with the best percentages were elected.

On 21 August 1964, the historic leader of the Italian Communist Party, Palmiro Togliatti died of cerebral haemorrhage[4] while vacationing with his companion Nilde Iotti in Yalta, then in the Soviet Union. According to some of his collaborators, Togliatti was travelling to the Soviet Union in order to give his support to Leonid Brezhnev's election as Nikita Khrushchev's successor at the head of Communist Party of the Soviet Union. Togliatti was replaced by Luigi Longo, a long-time prominent PCI members; Longo continued Togliatti's line, known as the "Italian road to Socialism", playing down the alliance between the Italian Communist Party and the USSR. He reacted without hostility to the new left movements that sprung up in 1968 and, among the leaders of the PCI, was one of those most disposed to engage with the new activists, although he did not condone their excesses.

In 1965, the SIFAR intelligence agency was transformed into the SID following an aborted coup d'état, Piano Solo, which was to give power to the Carabinieri, then headed by general De Lorenzo.

The difficult equilibrium of Italian society was challenged by a rising left-wing movement, in the wake of 1968 student unrest ("Sessantotto"). This movement was characterized by such heterogeneous events as revolts by jobless farm workers (Avola, Battipaglia 1969), occupations of Universities by students, social unrest in the large Northern factories (1969 autunno caldo, hot autumn). While conservative forces tried to roll back some of the social advances of the 1960s, and part of the military indulged in "sabre rattling" in order to intimidate progressive political forces, numerous left-wing activists became increasingly frustrated at social inequalities, while the myth of guerrilla (Che Guevara, the Uruguayan Tupamaros) and of the Chinese Maoist "cultural revolution" increasingly inspired extreme left-wing violent movements.

Seat distribution by constituency for the Chamber of Deputies (left) and Senate (right).

The election was a test for the new organization of the socialist area, which was divided between the new revolutionary and Communist-allied Italian Socialist Party of Proletarian Unity and the governmental social-democratic federation between PSI and PSDI. The polls said that the split of the PSIUP in 1964 had not been a purely parliamentary operation, but the reflex of divisions into the leftist electorate. The result shocked the PSI's leadership, causing the sudden sinking of the social-democratic federation, and an alternance of provisional retirements by the government, firstly led by lifetime senatorGiovanni Leone and then, through two political crisis, by DC's secretary Mariano Rumor. Unsuccessfully trying to recover its lost leftist electors, the PSI returned to the alliance with the PCI for the regional elections of 1970, so causing another crisis and a new change of premiership, then led by Emilio Colombo, but the government coalition had continuous problems of instability. Influent Giulio Andreotti tried to resurrect the centrist formula in 1972, but he failed, opening the way to the first early election of the republican history.