About Human ORFeome

The advent of systems biology necessitates the cloning of nearly entire sets of protein-encoding open reading frames (ORFs) collected into ORFeome collections, so as to allow functional studies of the corresponding proteomes. We present a new version of the human ORFeome, human ORFeome 8.1 containing clonally-derived and sequence-confirmed ORFs as a set of Gateway Entry clones ready for transfer to Gateway-compatible expression vectors.

hORFeome v8.1 represents a central resource of single-colony, fully-sequenced cloned human ORFs which can be readily transferred to Gateway compatible destination vectors for various functional proteomics studies. This set of ORFs ranges in size from 75 to more than 10,000 base pairs, and contains over 1,000 ORFs from genes with multiple splice variants.