Introduction

The MagicMark™ XP Western Protein Standard allows direct visualization of protein standard bands on a blot without the need for protein modification or special detection reagents. MagicMark™ XP Western Standard proteins are expressed in E. coli from a construct containing repetitive units of a fusion protein forming the size variation and an IgG binding site.

The important features of standard are listed below:

MagicMark™ XP consists of nine recombinant proteins in the range of 20–200 kDa

Directions for Use

The MagicMark™ XP Western Protein Standard is supplied in a ready-to-use format. There is no need to heat or reduce the standard prior to loading.

Load 2-10 µl of the standard on an appropriate SDS-PAGE mini-gel. We recommend testing different amounts of the standard to determine the optimal amount of standard to use under your experimental conditions.

The amount of standard will depend on the binding affinity of MagicMark™ for your antibody species and sensitivity of your detection system.