Asian redtail catfish is one of the most popular fish commodities in Indonesia. However, improvements in its aquaculture technology are still needed, especially in the breeding process. This study was aimed to evaluate the characters of reproductive biology and level of spawning on Asian redtail catfish from Cirata population incubated at different temperature settings of 23°C-24°C, 25°C-26°C, 27°C-28°C, and 29°C-30°C. Broodstock incubation was conducted in styrofoam boxes (sized 45 cm x 35 cm x 25 cm) with a wall thickness of 3 cm. One mature broodstock was placed inside each Styrofoam. Each styrofoam box was previously filled with freshwater of 20 cm deep, equipped with a lid on top, a water heater, and an aeration system. The gonad maturity stage of each broodstock was determined using cannulation, whilst the hormone induction used LHRH hormone analog (0.6 mL/kg). The injection was performed twice within six hours interval. The incubation was arranged in a completely randomized design (CRD) with four temperature treatments with three replicates. The results showed that the optimal incubation temperature for the broodstock was 27°C-28°C with the latent time ovulation of 8 hours 35 minutes and an ovulation rate of 100%. Statistically, the incubation of broodstock at 27°C-28°C showed a significant difference on the reproductive biological character value (P<0.05) compared to temperature ranges at 23°C-24°C and 25°C-26°C. The reproductive biology parameters generated were gonadosomatic index of 8.6 ± 0.5%; fecundity of 23,909 ± 1,473 egg per individual; fertilization rate of 85.5 ± 5.5%; hatching rate of 69.9 ± 5.0%; and survival rate of 72.3 ± 5.8%. At the incubation temperature of 27°C-28°C, the egg was distributed with an average egg diameter of 1.5 mm.