Mikhail Alexeyev (malexeye at JAGUAR1.USOUTHAL.EDU) wrote:
: This is being posted for a colleague of mine.
: I would like to insert a gene into specific region of a lambda phage. The
: idea is to subclone the lambda's region of interest in a plasmid,
: manipulate this region in an appropriate way and then return it back into
: the phage. Since lambda seems to lack appropriate restriction sites to do
: it conventionally, I think about using a homologous recombination as an
: alternative. The question is: how to go about crossing a phage and a
: plasmid?
Have a look at:
Boyd, A. C. & Sherratt, D. J. "The pCLIP plasmids: versatile cloning
vectors based on the bacteriophage &lambda origin of replication." Gene
(1995) 153, 57-62.
On-line pre-print available at
http://www.hgu.mrc.ac.uk/Users/Christopher.Boyd/ldv.htm
Best wishes,
--
Chris Boyd | from (but not \ MRC Human Genetics Unit
Christopher.Boyd at hgu.mrc.ac.uk | on behalf of) / Crewe Rd, Edinburgh
http://www.hgu.mrc.ac.uk/Users/Christopher.Boyd EH4 2XU, SCOTLAND