Custom antibody development

We offer world-leading antibody development platforms based on a proprietary RabMAb® rabbit monoclonal platform, a phase display platform (AxioMx), and a next-generation sequencing platform (NGS-RabMAb®)

Histone Extraction Kit (ab113476) is suitable for a quick preparation of total histone extracts from mammalian cells and tissue samples. The minimal amount of starting materials can be as low as 105 cells or 1 mg of tissue. For the best results, the cell number should be greater than 106 cells or the tissue amount should be greater than 10 mg. A total of 100 standard extractions (use 107 cells or 100 mg of tissue per extraction) can be performed with this kit. Yield of the total histone proteins can be up to 0.4 mg per 107 cells or per 100 mg of tissue. The yield may vary depending on the cell or tissue type.

The Histone Extraction Kit (ab113476) was used to extract histones from adult A. thaliana leaves.

2.5 g leaf material was frozen and ground in liquid N2 and used for the extraction with 8 ml 1x Pre-Lysis Buffer. Two filtration steps through 100 μm and 30 μm nylon meshes and additional Pre-Lysis Buffer wash steps were performed in order to remove residual plant material.The histone extract was precipitated with 4% perchloric acid o/n.

The Histone Extraction Kit is easy to use and generally works with A. thaliana leaves. However, the resulting sample contained some unexpected proteins, e.g. chloroplastic proteins, in addition to histones.

Our lab used the Histone Extraction Kit (ab113476) to extract histones from brains of the Western honeybee, Apis mellifera.

A single brain (approximately 10 mg) was used for each sample. We quantified the histone extracts using absorbance at 280 nm on a NanoDrop 1000 spectrophotometer. Each extraction yielded 20-40 ug of total histone protein. We then used the extracted histones in the Histone H3 Total Acetylation Detection Fast Kit (Fluorometric) (ab131561).

RIPA buffer can be used for extracting nuclear proteins but ab113476 is specifically for histone proteins. In general, the isolated histone protein concentration is much higher with use of ab113476than with RIPA buffer.

The EpiSeeker Histone Extraction kit was used to isolate histone proteins for use with the EpiSeeker Histone H3 Total Acetylation Detection Fast Kit. Histones were extracted pupae of the flesh fly, Sarcophaga bullata, which is a non-model insect.

The kit was easy and fast to use. It was easy to scale the reaction size to the amount of tissue.

This kit was used to isolate histones from pupae of the fleshy fly, Sarcophaga bullata, a non-model insect. A single pupa, weighing approximately 100 mg, was used in each sample. Each extraction yielded 14-19 ug/ul of histone protein. The kit was easy to use and simple to scale to the size of my insects. The extracted histones worked well with the EipSeeker Histone H3 Total Acetylation Detection Fast Kit.

Nuclear Protein extraction and Histone protein extraction from cell samples frozen at -80oC could still work using ab113474 and ab113476. However, the enzyme activity of nuclear extracts from frozen tissues may be much lower than that from fresh tissues.