Features

HFP and AFP detect selectively highly reactive oxygen species (hROS) such as hydroxyl radical (・OH) and peroxynitrite (ONOO－), whereas they do not react with other reactive oxygen species (O－・2 , H2O2, 1O2, NO, etc.).

Quantitative analysis of H2O2 is available by adding HRP.

Detecting specifically hypochlorite (OCl－) by the combination use of HPF and APF.

HPF and APF are not autoxidized at all, which enables us to easily get more reliable data than by other reagents.

Live fluorescence imaging of cells is available.

Principle of the measurement

HPF and APF are almost non-fluorescent in neutral solution. When they react with highly reactive oxygen species (hROS), they generate fluorescein that has high fluorescence intensity (excitation wave length: 490 nm, fluorescent wave length: 515 nm), and increase in the intensity of the fluorescence is observed.