Yeast Media, Amino Acid Dropout Mixes

These high-quality dropout supplements can be used together with SD base to create selective media for growing yeast cultures, confirming auxotrophic phenotypes, and selecting yeast transformants. They are ideal for use with Matchmaker Gold Systems and other yeast one-hybrid systems or yeast two-hybrid systems.

Dropout Supplements

Dropout (DO) supplements provide various mixtures of amino acids and nucleosides to add to the Minimal SD Bases to make a defined minimal medium. In each Dropout mix, certain amino acids have been “dropped out”, for example -Leu/-Trp DO supplement contains every essential amino acid except for leucine and tryptophan. DO Supplements may also be added to the minimal E. coli medium M9 for minimal selection in E. coli. Each package will make at least 10 L of medium.

Features

Dropout (DO) supplements for use with SD base to create selective media

Proven track record—15 years of yeast media sales

Applications

Confirmation of auxotrophic phenotypes

Selection for yeast transformants

Yeast two-hybrid screening

Yeast one-hybrid screening

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.