Holbrook, Scott, 1975-
2010-07-28T23:46:47Z
2010-07-28T23:46:47Z
2009-12
http://hdl.handle.net/1794/10553
xi, 56 p. : ill. (some col.) A print copy of this thesis is available through the UO Libraries. Search the library catalog for the location and call number.
The development of a functional nervous system is paramount for the ability of animals to interact with their environments. Minor defects in nervous system function compromise the effectiveness of sensing and responding to stimuli. Severe defects in nervous system function often lead to extreme sensory, cognitive and motor skill impairment. The nervous system is a complex network of connections, with each neuron making functional contacts with several other neurons. Any single animal species generally exhibits a stereotyped pattern of neuronal connectivity, but the specific intrinsic and extrinsic signals that impart to a neuron its unique connective properties have only recently begun to be identified.
In this study, we use the Drosophila visual system to examine neuronal connectivity. Our screen for genes involved in R7 photoreceptor connectivity led to the identification of the RhoGAP domain-containing protein dsyd-1 and the transcriptional repressor tramtrack . Flies harboring homozygous mutant dsyd-1 R7s fail to phototax towards UV light, an innate behavior mediated by the R7s. Subsequent analysis of axons of dsyd-1 R7s showed abnormal morphology in the region of presynaptic sites, suggesting that similar to its role in C. elegans , dsyd-1 is involved in presynaptic assembly. Further analysis demonstrated a requirement for dsyd-1 function in docking presynaptic components to terminal sites of contact.
R7 axons are restricted to non-overlapping columns in the optic neuropil, thereby preserving spatial visual information in the retintopic map. The axon terminals of tramtrack mutant R7s exhibit overgrowth, similar to that observed in R7s that have loss of function mutations in genes involved in the activin signaling pathway. Previous studies have shown that activin signaling is involved in restricting R7 axons to their appropriate columns, and our results demonstrate that tramtrack may be functioning in the same pathway. One of two tramtrack isoforms, ttk69 , is expressed in photoreceptors after they have differentiated, and expression of ttk69 is specifically required for R7 axon targeting.
Committee in charge: Eric Johnson, Chairperson, Biology;
Victoria Herman, Advisor, Biology;
Bruce Bowerman, Member, Biology;
Christopher Doe, Member, Biology;
Tom Stevens, Outside Member, Chemistry
en_US
University of Oregon
University of Oregon theses, Dept. of Biology, Ph. D., 2009;
Photoreceptor
Axon targeting
Presynaptic assembly
Genetics
Drosophila -- Nervous system
R7 photoreceptor axon targeting and presynaptic assembly in Drosophila
Thesis