Medical gloves are disposable gloves used during medical examinations and procedures that help prevent contamination between caregivers and patients. Medical gloves are made of different polymers including latex, nitrile rubber, vinyl and neoprene; they come unpowdered, or powdered with cornstarch to lubricate the gloves, making them easier to put on the hands. Cornstarch replaced tissue-irritating Lycopodium powder and talc, but since even cornstarch can impede healing if it gets into tissues (as during surgery), unpowdered gloves are being used more often during surgery and other sensitive procedures. Special manufacturing processes are used to compensate for the lack of powder. There are two main types of gloves: exam and surgical. Surgical gloves have more precise sizing with a better precision and sensitivity and are made to a higher standard.

In 1890 William Stewart Halsted was the first to use sterilized medical gloves when he was at Johns Hopkins University. With the publication of germ theory Halsted was using carbolic acid, introduced by Joseph Lister, to sterilize his hands and his nurse's hands. She was sensitive to the chemical, and it was damaging the skin on her hands; so he asked the Goodyear Tire and Rubber Company if they could make a glove of rubber that could be dipped in carbolic acid.

The first disposable latex medical gloves were manufactured in 1964 by Ansell. They based the production on the technique for making condoms.

Due to the increasing rate of latex allergy among health professionals, and in the general population, gloves made of non-latex materials such as vinyl, nitrile rubber or neoprene have become widely used. Chemical processes may be employed to reduce the amount of antigenic protein in Hevea latex, resulting in alternative natural-rubber-based materials such Vytex Natural Rubber Latex. However, non-latex gloves have not yet replaced latex gloves in surgical procedures, as gloves made of alternative materials generally do not fully match the fine control or greater sensitivity to touch available with latex surgical gloves. (High-grade Isoprene gloves are the only exception to this rule, as they have the same chemical structure as natural latex rubber. However, fully artificial polyisoprene—rather than 'hypoallergenic' cleaned natural latex rubber is also the most expensive natural latex substitute available.) Other high-grade non-latex gloves, such as neoprene gloves, can cost over twice the price of their latex counterparts, a fact that has often prevented switching to these alternative materials in cost-sensitive environments, such as many hospitals.

Powder-free medical gloves are used in medical cleanroom environments, where the need for cleanliness is often similar to that in a sensitive medical environment.