HELP
We are trying to PCR some highly conserved heat shock proteins and
are experiencing exceptional difficulty with PCR contamination.
We are aware that there are trace levels of DNA in many commercial preps
of TAQ and thought that it may be due to this.
We were thinking of DNAsing our TAQ fror these reactions or perhaps
DNAsing the entire mix (-primers) before the PCR. Would this work?
Has anyone done this before? Would it affect the PCR? (We've tried the
'low DNA' TAQ available already but with no success.)
Has anyone got any other ideas? or experience concerning PCRing
heatshock proteins or highly conserved sequences that would help?
Thanks for your time
JIM HARVEY