Efficient Midiprep Protocol (for HC plasmid)

Harvest the bacterial cells by centrifugation at 6000 x g for 15 min at 4°C.

Big centrifuge: SLA-1500; Rotor code 28; 6290 rpm.

Big centrifuge: HB6; Rotor code 23; 6060 rpm.

Alternatively, centrifuge cultures in 50 mL falcon tubes for 15 minutes at 4400 rmp in room 5096, and dump supernatant. Simultaneously, turn on the big centrifuge in room 5088, fill a bucket of ice, and place the medium rotor into the big centrifuge.