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The intended effect of the modification in 305423 soybean is to
produce soybean seeds with increased levels of monounsaturated
fatty acid (oleic) and decreased levels of polyunsaturated fatty
acids (linoleic and linolenic). To accomplish this objective,
Pioneer inserted a fragment of the soybean microsomal omega-6
desaturase gene (FAD2-1) into soybean. The fragment of the FAD2-1
gene does not code for a protein.

Transcription of the gene fragment under the control of a
seed-preferred KTi3 promoter acts to silence the expression of the
endogenous soybean omega-6 desaturase, which results in an
increased level of oleic acid and decreased levels of linoleic and
linolenic acids in the soybean seed. A gene (gm-hra) encoding a
modified version of the soybean acetolactate synthase (als gene)
that confers tolerance to a sulfonylurea herbicide was used as a
selectable marker for the transformation.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

The gm-fad2-1 cassette includes the promoter region from the
soybean Kunitz trypsin inhibitor gene (KTi3), a fragment of the
FAD2-1 gene that corresponds to approximately 40% of the middle
portion of the FAD2-1 gene (597 bp), and the 3' untranslated region
of the KTi3 gene (KTi3 terminator).

The gm-hra cassette contains the gm-hra gene (1971 bp), which is an
optimized form of the endogenous als gene from soybean, with
transcription regulated by the S-adenosyl-L-methionine synthetase
(SAMS) constitutive promoter (1.3 kb) from soybean and with
transcription terminated by the endogenous als gene terminator (0.6
kb) from soybean.

Sequence characterization of the inserted DNA in 305423 soybean
confirms that it contains four insertions that comprise:

* Insertion 1: one truncated PHP19340A fragment with
a truncated KTi3 terminator and intact gm-fad2-1 gene fragment and
intact KTi3 promoter, one intact PHP19340A fragment, one intact
PHP17752A fragment, one truncated PHP19340A fragment with an intact
KTi3 promoter and a truncated gm-fad2-1 gene fragment, and one
truncated PHP19340A fragment with a truncated KTi3 promoter and
truncated gm-fad2-1 gene fragment.
* Insertion 2: one truncated PHP19340A fragment with
a truncated KTi3 promoter and with intact gm-fad2-1 gene fragment
and intact KTi3 terminator.
* Insertion 3: one truncated copy of the KTi3
promoter with a nonfunctional 495 bp fragment of the plasmid
backbone; and
* Insertion 4: two truncated PHP19340A fragments in
an inverted repeat configuration, both with a truncated KTi3
promoter and intact gmfad2-1 gene fragment and KTi3
terminator.

The genetic material that is inserted in 305423 soybean is
genetically linked and segregates following a typical pattern of
Mendelian inheritance expected for a single, genetically-linked
insertion locus.