I have a 160 gig sata drive can i install it on its own ?
meaning i will have a 750 gig drive ata , and i also want to install this 160 gig drive as a sec drive , i also have a 350 gig ata drive extra as well , could i install these 2 extra dives along with my original 750 gig drive?

Just do them one at a time in case one of them doesn't want to play nice with the controller on the board. The only issues I've seen have been related to the Master/Slave relationship on the "run SATA in IDE mode" configuration of the motherboard.