ECL ghost bands

I apologize if this has been posted previously. We get many negative
images, or ghost bands, on our ECL exposures. Almost all the time we get
these things with the MW standards that were used; and then with certain,
but not all primaries. We're using PVDF, transfering in CAPS buffers, and
using amersham's ECL reagents. I suspect we should drop primary and
secondary amounts down a factor of 5 or 10 (now we use at 1:2000 primary;
1:10,000 secondary
Doug Feinstein
1819 West Polk St
MC 519, Rm 544
Chicago, IL 60612
fax: 815 333 0449
voice: 312 355 1665
email: dlfeins at uic.edu
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