OriGene Data

Anti-AKT2 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.

Clone Name

16G11.A7

Isotype

Species Reactivity

Human

Concentration

1.0 mg/mL

Guaranteed Application *

WB

Suggested Dilutions

ELISA: 1:2,000 - 1:10,000, WB: 1:500- 1:2000, IHC: 20 ug/mL

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Purification

Note

AKT2 Antibody detects AKT2 which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT2 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.