This assay employs an antibody specific for Human CEA coated on a 96-well plate. Standards and samples are pipetted into the wells and CEA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CEA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CEA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

RelevanceCarcino Embryonic Antigen (CEA) is synthesised during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).

The capture antibody in this kit is supplied by an outside lab and unfortunately they were not willing to provide me with any other details about it. They did want to know if you were interested in it to follow up ...

I can confirm that the kit should contain 8 ul of HRP-Streptavidin Concentrate (Item G). Please tell your customer to spin down the vial. The kit was ordered in November 2011 and we did change the amount of HRP-Str...

Unfortunately there is no cross reactivity data available testing the Human CEA ELISA Kit ab99992 with mouse. If it helps though, the capture antibody is a mouse monoclonal and reacts with the protein determinant belonging to the “gold” epitope group 4...

At the moment we do not sell kit components separately, this is because that we only produce enough reagents for use with the kit and do not have any spare to sell separately.
We may in future add the antibody in its own right but for now it can only ...

Thank you for contacting us.
I can confirm that all the mentioned kits (ab99992, ab108631, ab108635, ab108838) come with a 96-well strip plate (12x8). Each strip (12 total) has 8 wells so the customer can use a strip at a time if they wish.
I ...