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Description

Details

This antibody reacts with the stage-specific embryonic antigen-4 (SSEA-4), a glycolipid carbohydrate epitope that is expressed upon the surface of human teratocarcinoma stem (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells. No immunoreactivity is evident with undifferentiated murine EC, ES/iPS and EG cells. Expression of SSEA-4 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES/iPS cells may be accompanied by an increase in SSEA-4 expression.

Sample Data

Figure A: FC analysis on H1 human ES cells at a 1:100 dilution.Red histogram represents SSEA-4 Antibody and open histogram represents isotype control. A PE-conjugated anti-Mouse IgG was used as the secondary antibody. Figure B: ICC analysis on H1 human ES cells. Cells were stained with SSEA-4 Antibody at a 1:100 dilution followed by an Alexa 488-conjugated secondary antibody (green). Nuclei were counterstained with DAPI (blue).

Details

Details

Concentration

0.5 mg/mL

Species Reactivity

Human

Host

Mouse Monoclonal

Clone

MC-831-70

Isotype

IgG3

Immunogen

Human embryonal carcinoma cell line 2102Ep

Formulation

Aqueous buffer, 0.09% sodium azide.

Storage and Stability

Store at 2-8°C. Stable for 6 months from date of receipt when stored as directed.

Applications Tested

Flow Cytometry (FC), Immunocytochemistry/Immunofluorescence (ICC/IF)

Recommended Dilutions

Flow Cytometry 1:100 Immunocytochemistry/Immunofluorescence 1:100 It is recommended that the antibody be titrated for optimal performance for each application.