What proteases can be used for very specific cleavage of designed
cleavage sites in recombinant proteins? I know that thrombin,
enterokinase, and factor X sites have been used in expression vectors to
separate fusion proteins and to remove histidine tags. What conditions
optimize the chances for site-specific proteolysis? Are any other
enzymes used for this purpose? I am particularly interested in cleaving
a recombinant protein very near a membrane surface. Are
any proteases especially good at working in this environment?
Thanks for the help.
Gregg Wells
Department of Pathology
University of Pennsylvania
Philadelphia, Pennsylvania
USA
email: pathology at a1.mscf.upenn.edu