MTU 116 references ASM 227 as having already happened. (It also mentions Defenders 68, which isn't on Spidey's listing at all -- I don't have that issue, so can't check.)

DEF 68 occurred long before M/TU 116 and didn't feature Spidey.

Your conclusion about the placement of ASM 227 relative to ASM 226 makes sense to me, and I checked my copy of M/TU 116 to discover that, indeed, ASM 227 is noted in an editor's note as having already occurred. Thanks!