↵d Data are given for the two MiSeq runs separately. NCPCR, negative controls for PCR; NCext, negative controls for extraction; NCmus, negative controls for dissection; PCBartonella_t, positive controls for PCR; PCBorrelia_b and PCMycoplsma_m, positive controls for PCR and positive controls for indexing; TCC and TFA, thresholds for positivity for a particular bacterium according to bacterial OTU and MiSeq run (see also Fig. 1).