L6 Transfection Reagent (Myeloma Cells, CRL-1458)

Description

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Transfection Reagent for L6 Cells (Myeloma Cells, CRL-1458)

A biodegradable polymer based transfection agent – once inside the cell, the polymer degrades into smaller less toxic components reducing cell toxicity, facilitating release of the transgene, and improving transfection efficiency

The L6 cell line was established by Dr. Yaffe using rat (Rattus norvegicus) thigh muscle. These cells exhibit myoblast morphology and resemble skeletal muscle tissue. L6 cells have a unique ability to fuse, in a cell culture, to create multinucleated myotubes as well as striated fibers. These cells have been tested as negative for mousepox (ectromelia virus). These cells are often used in cell biology and molecular biology research.

Figure 2. Protein expression of Cyclophilin B in L6 cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into L6 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.