The CherryTemp™ fast temperature regulation system has been optimized for high resolution imaging in cell biology research. The ability of the CherryTempo™ temperature regulator to quickly modify the temperature of the biological sample under microscope observations has been applied to cytoskeleton polymerization. The compatibility of the CherryTemp™ temperature regulation system with high resolution microscopy enabled to study the polarity in fission yeast and mitochondria positioning. The fastness of the CherryTemp™ temperature regulation system has been also applied for thermosensitive proteins activation during imaging of cell on microscope.