Hi Brian:
> I know that there are some other cross-linkers for acrylamide.
> Are any of them cleavable? I'd think it would be handy to "digest" the
> gel and thus find it easier to get the protein out of it.
One suggestion I can make is the solubilizable acrylamide disulfide
gels made with disulfide containing crosslink bis-acrylylcystamine.
You can obtain this from BioRad. You just solubilize the gels by i
incubating with mercaptoethanol. You may want to look up the article
by Hanson, Analytical Biochem. 116:146-151.
Hope it helps.
Raj Shankarappa
bsh at med.pitt.edu
Univ of Pittsburgh
>> --
> ********************************************************************
> * Brian Foley * If we knew what we were doing *
> * Molecular Genetics Dept. * it wouldn't be called research *
> * University of Vermont * *
>