Recombinant Mouse Ntrk3encoding the extracellular domain of mouse TrkC (Met 1-Thr 429) was expressed, with a C-terminal His tag. It is Expressed inHuman Cells.

Description:

Neurotrophic tyrosine kinase receptor type 3, also known as NT-3 growth factor receptor, TrkC tyrosine kinase, TrkC and NTRK3, is a single-pass type I membrane protein. NTRK3 belongs to the protein kinase superfamily and insulin receptor subfamily. Mouse NTRK3 is a 825 amino acid (aa) protein and is widely expressed but mainly in nervous tissue. It contains two Ig-like C2-type (immunoglobulin-like) domains, one LRR (leucine-rich) repeats and one protein kinase domain. NTRK3 is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway.

Source:

Human Cells.

Purity:

>95% as determined by SDS-PAGE.

Endotoxin:

< 1.0 EU per 1μg of the protein as determined by the LAL method.

Stability:

Samples are stable for up to twelve months from date of receipt at -700C.

Predicted N Terminal:

Cys 32.

Molecular Mass:

The secreted recombinant mouse TrkC comprises 409 amino acids and has a calculated molecular mass of 46.1 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 100-110 kDa band in SDS-PAGE under reducing conditions.

Formulation:

Supplied as a 0.2μm filtered solution of PBS, pH 7.4.

Reconstitution:

Follow the instructions on the vial. Centrifuge the vial at 40C before opening to recover the entire contents.

Storage:

Store it under sterile conditions at -700C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.