We contracted out to a well known lab to have 2D gel
electrophoresis done on a HA tagged Human transient
receptor potential protein. Htrp and another protein cross
reacting with anti HA were spread across the pH gradient.
Htrp spread from 5 to 12.5 while the other unknown protein
was spread from 3.5 to 12.5. Samples were sent out twice
with identical results.
What could be the cause of these results?
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