The artificial insemination is rarely used for donkeys
and is still in the experimental stage. With fresh sperm the results
seem satisfactory, whereas in frozen spangles, the success is very
slight. Sperm samples were carried out in 2003 on two Asinerie's
stallions (Kyky du Bocage and Krypton du Bocage) by the laboratory
I.M.V to progress the research.

Insemination by frozen spangles would avoid the risks of consanguinity
and would improve the products which would make it possible for
small breeders very far away from the cradle of race to reproduce
a pure race.

Artificial vagina

Artificial vagina prepared to take stallion's
sperm sample.
We can notice this vagina has to be wormer to obtain a temperature
of approximately 45°C and the flask at the end is to
recover sperm..

Take sperm sample

A mare on heat call put the donkey in a position
and when he is jump on the mare, his penis is than introduced
into the artificial vagina until ejaculation.

Filtering

The filtering is to remove all impurities
which the penis could bring.

Microscope

Heating stage at 34-37°.
Observation between slide and coverglass to control the
mass mobility (spermatozoon movement) in pure semen.
We profit to evaluate spermatozoon concentration

Dilution

Dilution of the sperm with a special thinner enriched
in glycerol and egg yolk to prepare spermatozoon for the freezing.
Then, remains the work in laboratory to turn it in spangles.