We aim at understanding the principles by which membrane-associated scaffolds spatiotemporally organize exo-endocytosis, thereby making neurotransmission fast, efficient, and fault-tolerant over a wide range of stimulation frequencies. This will be done by conducting explorative computer experiments that model exo- and endocytic processes and the role of protein scaffolds by explicit space and time simulation of single protein copies. The simulations will be tightly integrated with the experimental work in the SFB.