Lumican is a secreted matrix protein that belongs to the small leucine­rich proteoglycan (SLRP) class II family. Mature Lumican has a negatively charged N-terminal domain that contains tyrosine sulfates, followed by twelve leucine-rich repeats, which are flanked by cysteine loops. Lumican is expressed widely in mammalian connective tissues, while corneal Lumican exists as a keratan sulfate proteoglycan in adult cartilage, Lumican exists primarily as a glycoprotein and lacks keratan sulfate. The aa sequence of human Lumican shares 88% aa sequence identity with that of mature mouse Lumican.

Catalog #

L6025-01

Applications

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution

Western Blot: 2ug/ml

Optimal dilutions to be determined by the researcher.

Storage and Stability

Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Clone Type

Monoclonal

Isotype

IgG2B

Clone No

12K129

Host

Mouse

Source

Human

Concentration

~0.5mg/ml after reconstitution

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.