Detection of a mycoplasma-like organism in peanut plants with witches'broom using indirect enzyme-linked imunosorbent assay (ELISA)

Author(s):Hobbs H A

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The mycoplasma-like organism (MLO) associated with peanut (groundnut) witches’ broom (PWB) from India was partially purified and an antiserum produced against it. Using a protein A indirect enzyme-linked immunosorbent assay (ELISA) procedure, PWB MLO was detected in crude extracts of leaves. stems and pegs of infected peanut plants, although stems were a better source than leaves and pegs. Extracts of infected tissues of three diseases of assumed MLO etiology in India, little leaf of brinjal (eggplant). I med rosed witches “broom, and Daturd sp. witches’ broom, failed to react with the PWB MLO antiserum.