Figure 6: CpG island methylation pattern at the E-cadherin gene in gastric mucosa from patients with dyspepsia. (a) Before eradication of H. pylori (week 0), methylation was present in patients 3, 5, and 6. (b) The methylated product was confirmed by sequencing using the same methylated primer. (c) After eradication of H. pylori (week 6), methylation was not present in any patient. (d) The methylated product was again confirmed by sequencing using the same methylated primer. No methylated cytosine was seen. MW: molecular weight marker, U: unmethylated band, M: methylated band, red color: unmethylated cytosines converted to thymidine, blue color: methylated cytosines. Taken from Chan and Rashid [45].