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AbSelect Serum Antibody Purification Kit Summary

Description

Antibodies generated from ascites fluid and serum are often supplied as crude formulations. Both ascites fluid and serum contain many substances that interfere with antibody labeling reactions. Successful labeling therefore requires antibodies to be purified prior to labeling. The AbSelect Serum system is a fast and simple method to purify antibodies from these types of media.

AbSelect Serum is prepared by coupling highly purified protein A to agarose beads which can be used to purify IgG fractions from ascites fluid and serum. The method involves the capture of the antibody on the AbSelect Serum resin and the removal of unwanted substances using a simple wash procedure. The purified product is then eluted and neutralized.

The components of the AbSelect Serum purification system are fully compatible with the Lightning-Link antibody labeling kits (available separately), which allows the purified antibody to be immediately labeled with an enzyme, fluorescent protein or fluorescent dye, with a hands-on time of under 30 seconds.

Benefits:

All components and buffers supplied

Purify up to 20 mg of antibody from ascites fluid or serum

Kit Type

Antibody Purification Kit

Packaging, Storage & Formulations

Storage

Store at 4C. Do not freeze.

Kit Components

Components

1 bottle of 10x Binding buffer

1 bottle of Elution buffer

1 bottle of Washing buffer

1 or 3 vials of AbSelect Serum Protein A resin

1 or 3 purification column(s)

1 vial of Neutralization buffer

Notes

AbSelect® is a registered trademark of Innova Biosciences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.