TSKgel PWXL Columns for Analysis of Water-Soluble Polymers

TSKgel PWXL columns are composed of spherical, hydrophilic polymethacrylate beads. The main application area for TSKgel PWXL columns is the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides. See the table on the TSKgel PW page for typical samples and suitable eluents.

Figure 1 shows the calibration curves for all TSKgel PWXL-type columns. Figure 2 shows the benefits of TSKgel PWXL columns over TSKgel PW columns, namely the improved resolution due to the smaller particle sizes used in TSKgel PWXL columns.

Figure 1: Polyethylene glycol and oxide

calibration curves on TSKgel PWXL columns

Although proteins can be analyzed on TSKgel PWXL columns, pore volumes are much smaller than on TSKgel SW-type columns. Figure 3 demonstrates the limited separation power of TSKgel PWXL for protein samples.

Specialty Columns

TSKgel G-DNA-PW is available for the analysis of DNA fragments. Similarly, TSKgel G-Oligo-PW is the preferred column for the analysis of oligosaccharides.

The mixed-mode column, TSKgel GMPWXL, has an extended linear calibration range, suitable for samples with a broad MW distribution and unknowns.

A smaller particle size version of the popular TSKgel G-Oligo-PW column for oligomer separation is now also available. By using 3 micron instead of 7 micron particles a separation performed on an TSKgel G-Oligo-PW column can be done in half the time on a TSKgel SuperOligoPW column.