HeLa cells expressing iRFP were seeded onto 96-well plates and incubated with buffer or different concentrations of SB743921.During the next ﬁve days, the plate was scanned with an infrared imaging system (upper panel) to quantify the infrared signals (lower panel).

Following single thymidine arrest (STA) for 24 h (the numbers above the arrows indicate the time in hours), cells were released into nocodazole (Noc) for 12 h and then treated with DMSO or Noc plus MG132 (to prevent mitotic exit). After 2 h, cells were processed for immunoprecipitation (IP). cells were synchronized using AZ3146 or other small molecule inhibitors. Cell lysates were then analyzed for BUBR1. Noc, nocodazole.

HeLa cells expressing iRFP were seeded onto 96-well plates and incubated with buffer or different concentrations of SB743921.During the next ﬁve days, the plate was scanned with an infrared imaging system (upper panel) to quantify the infrared signals (lower panel).

Following single thymidine arrest (STA) for 24 h (the numbers above the arrows indicate the time in hours), cells were released into nocodazole (Noc) for 12 h and then treated with DMSO or Noc plus MG132 (to prevent mitotic exit). After 2 h, cells were processed for immunoprecipitation (IP). cells were synchronized using AZ3146 or other small molecule inhibitors. Cell lysates were then analyzed for BUBR1. Noc, nocodazole.