In article <1993Mar9.140331.203720 at uctvax.uct.ac.za>,
blatch at uctvax.uct.ac.za writes:
> PROBLEM WITH ELUTION CONDITIONS FOR A HYDROPHOBIC COLUMN:
>.
.
.
> hydrophobic moiety? Any suggestions will be greatly appreciated.
> Please send suggestions to me or Neil at micro.uct.ac.za>>>
You might try using a non-ionic detergent such as Tween 20 to elute
your material. I have had success with this on Pharmacia
Phenyl-superose columns. The inclusion of 0.02% Tween 20 in the
elution buffer has both given me better yields of some proteins and
removed others that otherwise needed acetonitrile to get them off the
column. The elution position will be somewhat advanced but this need
not be a problem.
--
Craig Marshall bioc07 at otago.ac.nz
Biochemistry Department
University of Otago Phone 64 3 479 7849
P.O. Box 56 Fax 64 3 479 7866
Dunedin, New Zealand