Hi,
I try to write a short program to generate position specific score matrix
(PSSM) from a multi-alignment of sequences in Perl language, I know there are
methods to do this, but I want to do this like PSI-blast. Unfortunately, the
original article about PSI-blast does not make me clear, and the NCBI source
code is too difficult to read.
Anyone who can tell me the its principle and how psi-blast works?
Thanks.
Jarod