Custom antibody development

We offer world-leading antibody development platforms based on a proprietary RabMAb® rabbit monoclonal platform, a phase display platform (AxioMx), and a next-generation sequencing platform (NGS-RabMAb®)

Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

ICC/IF

1/500.

WB

1/1000. Detects a band of approximately 220 kDa (predicted molecular weight: 238 kDa).

IHC-Fr

Use at an assay dependent concentration.

IHC-P

Use at an assay dependent concentration.

IP

1/10 - 1/500.

Target

Function

May be a structural component of the nucleus.

Cellular localization

Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.

Anti-NuMA antibody images

Immunocytochemistry/ Immunofluorescence - NuMA antibody (ab36999)

ab36999 at 1/1000 dilution, staining human HeLa cells fixed with 3.5% formaldehyde, by immunofluorescence, showing the typical pattern for NuMa in the cell nucleus during interphase and on spindles during mitosis.