AnaSpec’s
SensoLyte® MMP assay kits, especially the SensoLyte® 520
MMP Assay Kits, have become the new standard in MMP assay sensitivity.
With published results in world-class journals and the industry’s longest wavelength,
the SensoLyte® 520 line is one of the most sensitive
(Figure 1) and brightest lines of MMP assay kits in the world.

… genetically alteredcells (esophageal squamous) were
used to detect MMP-9 enzymatic activity in duplicate using the SenzoLyte
Plus MMP-9 assay kit from AnaSpec.9

Rat primary microglia (5 × 106 cells/mL) were
treated with LPS/PMA in the presence or absence of MMP inhibitors for
24 h and the supernatants were collected for measuring MMP activities.
Both the total and active MMP-3 or MMP-9 activities were assayed according
to SensoLyteTM 520 MMP-3 or SensoLyte PlusTM 520
MMP-9 assay system, respectively (AnaSpec, San jose, CA, USA). 10

General MMP activity was assessed using the EnzoLyte™ 520 Generic
MMP assay Kit (AnaSpec, San Jose, CA). This kit is optimized to detect
the activity of different MMPs such as MMP-1, -2, -3, -7, -8, -9, -12,
-13 and -14, using a fluorescence-quenched substrate (5-FAM/QXL™520).15

WT mice of the genetic background C57BL/6 were obtained fromJackson Laboratories…MMP activity was determined using the SensoLyte520 GenericMMP Assay Kit (AnaSpec) as per manufacturer's
instructions.Briefly, media from WT, TIMP-3–/–,
and WT cellstreated with rTIMP-3 (1 µg/ml) were collected
72 h postseeding,and 50 µl of these media were incubated with
the FAM/QXL520 fluorescence resonance energy transfer substrate
for 1 hin a black 96-well plate at room temperature in the
dark. 17

Thirty human fibroblast populations were isolated from the skin
biopsies of SSc(Systemic sclerosis) patients…Total MMP activity was determined
in the same supernatant as that for collagen assay using the SensoLyteTM
520 Generic MMP assay kit (Anaspec, San Jose, CA, USA). This kit can detect
simultaneously the activity of MMP-1, 2, 7, 8, 9, 12, 13 and 14.18

Rat primary microglia (5 × 106
cells/mL) were treated with LPS/PMA in the presence or absence of MMP
inhibitors for 24 h and the supernatants were collected for measuring
MMP activities. Both the total and active MMP-3 or MMP-9 activities were
assayed according to SensoLyteTM 520 MMP-3 or SensoLyte PlusTM
520 MMP-9 assay system, respectively (AnaSpec, San Jose, CA, USA).10