This study was to determine whether GM-CSF induced WT1 gene expression and
to establish an association with markers of proliferation CD71+CD34+ using nPCR and flow cytometry respectively, in samples
obtained from 5 newly diagnosed JMML patients. Overtime (day 0 to day 14) there
was an insignificant difference in WT1 gene expression and CD71+CD34+ in JMML
samples when compared to peripheral blood of normal volunteers (n = 3). Our
study suggests that there is a correlation between WT1 gene expression and cellular
proliferation and that GMCSF in vitro does not create a significant difference in JMML samples.

Kletzel, M., Olzewski, M., Huang, W. and Chou, P.M. (2002) Utility of WT1 as a reliable tool for the detection of minimal residual disease in children with leukemia. Pediatric and Developmental Pathology, 5, 269-275.http://dx.doi.org/10.1007/s10024-001-0208-x