Rocky Baker wrote:
>> What is the stability of a protein solubilization solution containing
> Tris-Hcl, 4% SDS, 20% glycerol, and 10% 2-mercaptoethanol?
Rocky,
I'd be worried about the b-merc oxidizining, so would add that only as I
need the buffer. The other components should be fine although you may
want to filter sterilize because of the glucose and I would not store it
at 4C as the SDS will probably come out of solution.
Good luck,
Randy
Randall C Willis, Researcher
Biochemistry Research, Hospital for Sick Children
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