An experiment was carried out at newly developmental farm (horticulture section) situated at University of Agriculture, Peshawar during the year 2012 to see whether the influence of nitrogen level and planting time on growth of Aloe vera plant was or not. The experiment was carried out using Randomized Complete Block Design with split plot arrangement. There were four levels of nitrogen (0, 50, 100 and 150 Kg ha-1) and four planting times (20th March, 04th April, 20th April and 04th May, 2012). The Urea (46% nitrogen) was applied as a source of nitrogen. Nitrogen levels significantly affected all of the parameters except survival percentage while planting time affected all parameters significantly. The tallest plant height (32cm), highest amount of gel leaf-1 (21.75 gm), highest leaf breadth (3.83cm),highest leaf thickness (1.67cm), High number of suckers plant-1 (9.83), highest leaf volume (34.77 cm3), highest number of leaves (7), heavier leaf weight (32.08 gm) were observed in suckers treated with 150 kg nitrogen ha-1. While Survival percentage was not significantly affected by nitrogen. However, planting time significantly affected all parameters. Maximum plant height (27.7cm), high amount of gel leaf-1(13.8 gm), highest leaf breadth (2.98cm),highest leaf thickness (1.67cm), highest number of suckers plant-1 (9.17), highest leaf volume (37.04cm3) highest number of leaves (6),heavier leaf weight (31.32 gm), survival percentage (100 %) were observed in suckers transplanted on 20th March. Nitrogen level at 150 kg ha-1 and 20th March as transplantation resulted best performance and hence recommended for establishment and growth of Aloe vera plant.