Introduction: Fungal infections know an extraordinary expansion with therapeutic progress and apparition of immunodepressive diseases such as HIV. Every year, new pathogenic species are identified. Vaginal candidiasis is a part of genital activity woman's life and Candida albicans is the most frequently isolated species. The intervening of infections due to strains of “non albicansCandida” resistant to current antifungal drugs increases the frequency of the relapsing vaginal candidiasis. We report here two cases of vaginal infections due to Stephanoascus ciferrii in Clinique Biasa in Lomé (Togo).Materials and methods: Vaginal secretions were cultured on Sabouraud – Chloramphénicol agar. Identification and antifungal susceptibility have been achieved on “VITEK® 2 Systems” (bioMérieux®, France) using YST® and AST-YS01® cards. The susceptibility to four antifungal drugs (amphotéricine B, fluconazole, flucytosine and voriconazole) has been tested at four different concentrations.Results: On Sabouraud - Chloramphenicol agar has been gotten creamy colonies. In the two cases Stephanoascus ciferrii is identified. The antifungal susceptibility test revealed sensitivity to the four antifungal drugs tested with a precise determination of minimal inhibitory concentrations (MIC).Conclusion: The use of an automated and standardized system as the Vitek® 2 was decisive for isolation of Stephanoascus ciferrii. It is necessary to keep in mind that, unusual germs can cause vaginal infections.