Application Information: IL1 alpha antibody [ILA9-H18]

0.98
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Protein G

Specifications: IL1 alpha antibody [ILA9-H18]

Full Name

interleukin 1, alpha

Product Description

Mouse monoclonal antibody [ILA9-H18] to IL1 alpha

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]

Sandwich ELISA analysis of human IL1 alpha protein in dodecuplicate at 400, 160, 64, 25.6, 10.25 and 0 pg/ml (50ul per well) using GTX15754 as capture antibody (3ug/ml) and GTX15753 as capture antibody (0.15ug/ml). The plate was washed and incubated with Streptavidin-HRP. Detection was performed using TMB Substrate. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.

Sandwich ELISA analysis of human IL1 alpha protein in dodecuplicate at 400, 160, 64, 25.6, 10.25 and 0 pg/ml (50ul per well) using GTX15754 as capture antibody (3ug/ml) and GTX15753 as capture antibody (0.15ug/ml). The plate was washed and incubated with Streptavidin-HRP. Detection was performed using TMB Substrate. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.