OBJECTIVE: This study compares the effects of strength training (ST) with or without supplementation of omega-3 lipids (W-3) or medium chain triglycerides (MCT) in metabolic indicators in the exhaustion test (ET). METHODS: The subjects 12 males with minimum 11 months of ST of experience were divided in group W-3 (GW-3: n = 7, 26.7 ± 6.0 years old; 82.6 ± 10 kg) and group MCT (GMCT: n = 5, 18.8 ± 1.3 years old; 74.6 ± 9.7 kg). There were 2 moments of ET: after 28 days only with ST and blood collection samples before (M1b) and after (M1a), and after 28 more days of ST plus supplementation with 4 g/d of W-3 or 4 g/d of MCT repeating the same procedure (M2b and M2a). RESULTS: The hematocrite (Ht), osmolality (Os), sodium (Na+) and PCO2 showed no significant change at any moment, group or D M2 (P > 0.05). The PO2 increased significantly after ET in two moments and groups (P < 0.05). The glucose and HCO3 had significant increase in M1 to GW-3 and HCO3 in M2 to GMCT (P < 0.05), without other changes. The LDH increased significantly only in M2 to GW-3 (P < 0.05) and pH decreased in M1 to both groups (P < 0.05), without other significant changes. CONCLUSIONS: The ET per se altered the major metabolic indicators but a great standard deviation occurred. The tests induced acidosis without influencing fat acids supplementation.