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Methods: Surface markers and intracellular cytokines were measured with flow cytometry and sST2 and sCD14 with ELISA.

Results: The group who experienced symptoms when working with LA, with positive and negative skin-prick test to LA, had higher CD14 expression on monocytes compared with those allergic to birch and controls. Further, serum sST2 were elevated in birch atopics and in symptomatics non-allergic to LA compared with controls.

Conclusion: Increased CD14 expression found in LA workers is most likely a response to non-allergic agent exposure whereas ST2 seems to react to acute allergen exposure and to non-allergic stimuli as pathogen-associated molecular patterns.

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