Protein A orginates from Staphylococcus aureus and has five antibody binding domains and a cell binding domain. It binds IgG from most mammalian species and some IgA and IgM through the Fc region.

Medicagos recombinant protein A, "PAII" is produces in E. coli using animal free media. The recombainat form has the cellbinding domain removed, for reduced nonspecified binding and leakage.

PAII is supplied as a lyophilized white flocculate. The purity ofPAII is determined by SDS- PAGE, which generates one single band at 42 kDa. The activity is tested by interaction with human igG. The product is to be used for laboratory work only.

Applications

Purification of polyclonal and monoclonal antibodies

Immunoprecipitation

Directions for use

Dissolve the protein in desired buffer depending on your application.

Shipping and storage

The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than three years from production date when stored below -20°C. After reconstitution, the solution may be stored frozen in working aliquots for up to 12 months.