Flow Cytometry. Immunofluorescence. Inhibits Binding of NGF to Melanoma Cells (use antibody without azide). Immunohistology on formalin-fixed, paraffin-embedded tissue sections (use 1-2µg/ml for 30 min at RT; Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0). Not suitable for Western Blotting.Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.

Concentration

0.2 mg/ml

General Readings

Marano N, et. al. Journ

Storage

Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.

Format

State:

Purified

Specificity

Specificity:

This antibody reacts with the NGF Receptor.NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two di stinct receptors, the high affinity p140 and low affinity Nerve Growth Factor (NGF) Receptor (p75 NGFR) or Neurotrophin Receptor (p75NTR). Recommended Positive Control: Melanoma. Cellular Localization: Cell Surface, Cytoplasmic. Species Reactivity: Human, Monkey (Cebus apella, Macaca fascicularis, mulatta, and nemestrina), Baboon (Papio anubis and Cynocephalus), Cat, Rabbit, and Ferret. Does not react with Mouse and Rat. Others not tested.