The binding sites of Manganese atom in the structure of W174F Mutant Creatininase, Type II (pdb code 3a6f). This binding sites where shown with 5.0 Angstroms radius around Manganese atom. The 3a6f structure was solved by Y.NAKAJIMA, K.YAMASHITA, K.ITO, T.YOSHIMOTO, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: