Dr. Anita Pandey Delivered the lecture on Microbial resources and their application In Uttaranchal (P.G) College of Biomedical Science & Hospital, Dehradun, on the topic of the uses of Microbiology, Biotechnology, environments, agriculture, industries, distilleries, water plant, in aquatic system, in our river, in temperate region, role of microbes in extreme cold and extreme hot region at sea level and about many more areas.

ABOUTGBPIHED

(Autonomous Institute of MoEF&CC)

Mandate: Indian Himalayan Region

Research and Development division

Almora (HQ), Uttarakhand

Srinagar, Garhwal, Uttarakhand

Kullu, Himachal Pradesh

Panthang, Tadong, Sikkim

Itanagar, NE, Arunachal Pradesh

New Delhi

Microorganisms are ubiquitous in nature…

The ubiquity of microorganisms is mainly attributed to:

the small size and their easy dispersal

their metabolic versatility and flexibility

to utilize wide substrates as nutrient source

ability to survive and multiply in diverse habitats,

including anaerobic

Microbial Diversity and Biotechnological Applications

Nutrient cycling (decomposers)

Medicine

Industry

Agriculture- biological fertilizers

Fermentation- foods and beverages

Biotechnological tools- genetic engineering

Dr. Anita Pandey delivered a lecture on Microbial Resource and Bioprospecting. Our UCBMSH students from different course i.e. M.Sc. Microbiology, B.M.M, Agriculture IIndsem Students, M.Sc. Zoology students, and B.P.T. IInd year students attained the lecture. Where they get more information about the world of microbes and their uses in day to day life.

Dr. Anita Pandey discussed on various topics in Microbiology and also she gave a huge knowledge about research field in microbiology and some ideas about the research. She discussed on the topic are follow:

Bioprospecting of microbial resources –Microbial Diversity

Applications- Screening of biomolecule

e.g., antimicrobials, microbial enzymes, pigments

Basic Techniques

Research areas

Important in medicine, agriculture, and industry

Physical conditions

Temperature (Mesophiles, psychrophiles, thermophiles)

pH (Acidophiles, alkaliphiles)

Oxygen (Aerobic, anaerobic)

>Pure cultures

Enzymatic activities

Molecular characters

DNA based characters

Isolation of DNA

Purity testing (for contaminants: mRNA, proteins)

electrophoresis (for presence of DNA) &

spectrophotometer (for purity and conc of DNA)

Removal of contaminants using RNAse and Proteinase K

PCR (amplification of isolated DNA using universal or specific primers)

Electrophoretic and

Gel documentation (size of base pair)

analysis of the PCR product (sequencing)

Sequencing

DNA Sequencing

Identification: Sequencing of conserved region of rRNA

Bacteria: 16S

Fungi 18S or ITS (internal transcribed sequence) region

Similarity index from the Database

Metagenomics

Isolation of DNA from soil

Denature Gradient Gel Electrophoresis

Research areas
Himalaya
represents one of the
34 globally recognized biodiversity hotspots and
a discrete geographic and ecological entity