This application note describes a method of extraction for metanephrine and normetanephrine from synthetic
urine using EVOLUTE® EXPRESS WCX 96-well SPE plates.
A new solid phase extraction technology has been developed to reduce
the time associated with sample preparation methods by
eliminating steps in the extraction workflow. Leveraging this
technology, a method protocol was developed using a load,
wash and elute approach. This method was optimized for
synthetic urine.Tags: 96-well plate,
Application Notes,
Catecholamines,
Clinical,
English,
LC-MS/MS,
Metanephrine,
Normetanephrine,
Synthetic urine

This application note describes a method of extraction for metanephrine and normetanephrine from pooled
plasma using EVOLUTE® EXPRESS WCX 96-well SPE plates.
A new solid phase extraction
technology has been developed to reduce the time
associated with sample preparation methods by eliminating
traditional steps in the extraction workflow. Leveraging this
technology, a method protocol was developed using a load,
wash and elute approach. This method was optimized for
pooled plasma.Tags: 96-well plate,
Application Notes,
Catecholamines,
Clinical,
English,
LC-MS/MS,
Metanephrine,
Normetanephrine,
Plasma

EVOLUTE® EXPRESS Solid Phase Extraction products
from Biotage are designed for all your sample
preparation challenges. Whether you are working in the field of bio-analysis during drug development, clinical or forensic
toxicology, or with the diversity of samples in food
safety and environmental applications, the tools and
methods used for sample preparation have a clear
impact on productivity. This user guide contains information about choosing the correct EVOLUTE EXPRESS product for your application and optimizing your methodology for best results.Tags: 96-well plate,
Clinical,
Column,
English,
Environmental,
Food,
Forensic,
Manuals & User Guides,
On-line SPE,
Pharma

Part of the B complex group of water soluble vitamins, vitamin B7 (Biotin) is involved in various metabolic processes within the body. It is a small polar carboxylic acid which can present challenges in bioanalysis. This poster discusses the impact of optimization of various parts of the method development process to maximize
sensitivity allowing low level analysis of vitamin B7 from human serum.
MSACL 2017, Palm Springs
ASMS 2017, IndianapolisTags: 96-well plate,
Biotin,
Clinical,
English,
LC-MS/MS,
Posters,
Serum,
Vitamin B7,
Vitamins,
Water Soluble Vitamins

This poster examines various factors in the development and optimisation of a SPE-LC-MS/MS method for extraction of catecholamines from plasma.
The final method utilizes EVOLUTE EXPRESS WCX plates in either standard SPE or fast Load-wash-elute modes, and incorporates a series of wash steps to optimise analytical sensitivity. Low elution volumes mean no evaporation step is required, and LLOQ is 20mg/mL or lower for each of the analytes.Tags: 96-well plate,
Catecholamines,
Clinical,
Dopamine,
English,
Epinephrine,
LC-MS/MS,
Norepinephrine,
Plasma,
Posters

This report details a “load, wash, elute” weak cation exchange solid phase extraction procedure amenable to both plasma and urine samples. The extracts are subsequently injected into an LC-MS/MS system. The preliminary sample preparation method was developed at the Biotage US Applications lab (Charlotte, NC).The method was then transferred to Ionics (Bolton, ON, Canada) to facilitate the nmole/L measurements of the selected biomarkers
by laminar flow tandem mass spectrometry. The SPE-LC-ESIMS/ MS method parameters were first optimized using pooled mixed gender plasma. A set of patient samples (n=32) was later supplied by the Mayo Clinic (Rochester, MN) that had previously been analyzed by a validated referee method. The population represented measured values across a range of clinical relevance.
AACC 2014Tags: 96-well plate,
Catecholamines,
Clinical,
English,
LC-MS/MS,
Metanephrine,
Normetanephrine,
Plasma,
Posters,
Urine

This poster focuses on the different strategies that can be employed when using EVOLUTE SPE sorbents (with both single and dual retention mechanisms) to reduce or eliminate residual phospholipids in sample extracts.
HPLC 2014Tags: 96-well plate,
Clinical,
English,
Forensic,
LC-MS/MS,
Pharma,
Posters

In this poster, an IONICS 3Q 220 triple quadrupole mass spectrometer using a load, wash, elute method protocol with recently advanced solid phase extraction (SPE) sorbent technology (EVOLUTE EXPRESS WCX) was used to perform analysis of the two compounds. As a result low levels of metanephrine and normetanephrine are detectable in plasma with short sample preparation and LC run times. This LC-MS/MS method provides a fast, sensitive, accurate, and reproducible solution for the analysis of ME and NME in plasma.
MSACL 2014Tags: 96-well plate,
Clinical,
English,
LC-MS/MS,
Metanephrine,
Normetanephrine,
Plasma,
Posters

EXPRESS represents a leap forward in high
throughput solid phase extraction. The EVOLUTE
EXPRESS range of 96-well SPE plates combines
powerful EVOLUTE sorbent chemistry with innovative
features that enhance productivity by optimizing
or even eliminating the need for some traditional
steps in the SPE procedure. By truly eliminating the
need for conditioning and equilibration, samples
can be prepared by loading the plate, washing away
interferences and eluting with compatible solventsTags: English,
Product Notes

About Biotage

Biotage is a global Life Science company that develops innovative and effective solutions for separation within organic and analytical chemistry, as well as for industrial applications. Biotage is listed on NASDAQ Stockholm.