Research: Shared Services

Genomics Shared Service: Services

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Peptide Synthesis

Peptide synthesis is supported by a Rainin Symphony 12 channel multiple peptide synthesizer, allowing for synthesis at variable scales ranging from 25mMol to0.5mMol. Additionally, a Protein Technologies Prelude, which was beta-tested in this facility, providessynthesis at smaller scales (1 to 10mMol). Turn around times for synthetic peptides range from 2-3 weeks, depending on whether purification is requested. A variety of modified amino acids can be incorporated, including hydroxyproline, methylated amino acids, andnon-standard amino acids such as ornithine and aminobutryic acid. The Genomics Shared Service has been very successful in phospho-peptide synthesis, as well as development of synthesis strategies to effectively label peptides with a variety of labels including biotin, fluorescein and rhodamine derivatives, and incorporating multiple labels in a peptide. Other specialty services that the facility excels at include cyclo-peptide synthesis via amide-bond formation and disulfide bridging, synthesis of multi-branched, dendrimer-like peptides, and synthesis of peptide-DNA hybrids. Manual synthesis is employed for small di- or tri-peptides.

The facility has done a great deal of large-scale peptide synthesis and purification, including peptides included in the National Institutes of Standards and Technology Reference Materials RM8327, Peptide Standards for Molecular Mass and Purity Measurements.

Peptide purification is available with a choice of two levels of purity, >70% and >95%. Purification is done by reversed phase HPLC, although ion exchange chromatography is available if necessary. Peptides are provided as lyophilized powder, with gross weights provided. Provided along with the peptide are the analytical data, both HPLC and MALDI-TOF mass spectra. The cost for synthesis (without purification) or synthesis and purification to the two levels of purity are detailed in the chart below as a cost per amino acid.

Peptide Purity Level

10μM

25μMole

50μMole

100μMole

250μMole

Crude Peptide

$10.00 *

$15.00

$20.00

$30.00

$50.00

>75% Peptide Purity

N/A

$25.00

$30.00

$40.00

$60.00

>95% Peptide Purity

N/A

$35.00

$40.00

$55.00

$75.00

* For 10μMole scale, purification, if desired, is a separate charge of $100 per peptide.

This page was last updated on: January 10, 2011.

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