Application Information: CEA antibody [C6G9]

Dot: Use at a dilution of 1/8000. IHC-P: Use at a dilution of 1/8000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. WB: Use at a dilution of 1/8000. Predicted molecular weight: 180 kDa. There is no cross reactivity with nonspecific cross-reacting antigen (NCA) present in granulocytes by immunoblotting, flow cytometry or immunohistochemical techniques. The antibody recognizes an epitope that is resistant to 30 minute oxidation by 1% sodium periodate solution in routine formalin-fixed, paraffin-embedded tissue sections. Enzymatic predigestion with proteolytic enzymes enhances immunohistochemical staining of formalinfixed, paraffin-embedded tissue sections with the antibody. Optimal dilutions/concentrations should be determined by the end user.

Form Supplied

Liquid

Purification

Ascites

Specifications: CEA antibody [C6G9]

Full Name

carcinoembryonic antigen-related cell adhesion molecule 5

Product Description

Mouse monoclonal [C6G9] to Carcino Embryonic Antigen CEA

Background

Carcino Embryonic Antigen (CEA) is synthesized during development in the fetal gut, and is re expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).

Synonyms

P06731, CEACAM5, 114890, CD66e, 1048, DKFZp781M2392, CEA

Cellular Localization

Plasma membrane

Specificity

Monoclonal Anti-Human Carcinoembryonic Antigen (CEA) reacts specifically with human CEA (CD66e, 180 kDa) from several types of malignant tissues including colorectal, lung and breast tumors. It also stains medullary carcinomas of the thyroid. The product reacts strongly with the cell surface and cytoplasm of malignant glands in colorectal adenocarcinomas. A weak reactivity is seen with normal colon mucosa and occasionally with bile canalicular and pancreatic acinar cells, but not with other tested normal tissues including term placenta.

Host

Mouse

Clonality

Monoclonal

Clone Name

C6G9

Target

CEA

Isotype

IgG1

Immunogen

CEA isolated from a human colon adenocarcinoma cell line.

Antigen Species

Human

Species Reactivity

Human

Storage Conditions: CEA antibody [C6G9]

Storage Buffer

Preservative: 0.1% Sodium Azide

Storage Instruction

Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Notes

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.