Ni-NTA HRP Conjugate

For simple, direct detection of His-tagged proteins without secondary antibodies

Direct detection of His-tagged proteins

Streamlined detection, eliminating the need for antibodies

Sensitive detection using small quantities of conjugate

Ni-NTA HRP Conjugate is used to detect recombinant proteins carrying His tags without the need for antibodies. The Ni-NTA moiety binds to a protein's His tag and the complex is localized using chromogenic substrates that react with conjugated horseradish peroxidase (HRP).

* Detection using chromogenic substrate. Sensitivity may vary with different proteins

Principle

Ni-NTA HRP Conjugate consists of Ni-NTA coupled to horseradish peroxidase (HRP). It can be used for direct, straightforward detection of any recombinant protein with an accessible His tag. It eliminates the need for secondary antibodies, thereby saving time in detection procedures. Ni-NTA HRP Conjugate is recommended for use with chromogenic substrates, and for E. coli expression systems. The use of chemiluminescent substrates with Ni-NTA conjugates is not recommended.

Applications

Ni-NTA HRP Conjugates are recommended for detection of proteins in E.coli by western, dot, and colony blotting procedures.