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Publications25

Specific in vivo labeling of tyrosinated α-tubulin and measurement of microtubule dynamics using a GFP tagged, cytoplasmically expressed recombinant antibody

Lynne CassimerisDepartment of Biological Sciences, Lehigh University, Bethlehem, Pennsylvania, United States of AmericaPLoS ONE 8:e59812. 2013

..Thus, a recombinant antibody with weak affinity for its substrate can be used as a specific intracellular biosensor that can differentiate between unmodified and post-translationally modified forms of a protein...

..New studies measure the force produced by depolymerization and show that protein ring complexes can couple depolymerization to movement. These results have implications for anaphase chromosome motility and mitotic evolution...

..In general, although some microtubule-stabilizing proteins are inactivated at entry into mitosis, a critical balance between microtubule stabilizers and destabilizers is necessary for assembly of the mitotic spindle...

XMAP215 is a long thin molecule that does not increase microtubule stiffness

..Because Rac1 mediates persistent growth of microtubules to the lamellar regions of cells, we propose that Ad-induced activation of Rac1 enhances the ability of microtubules to "search and capture" incoming virus particles...

Estimates of lateral and longitudinal bond energies within the microtubule lattice

..Cluster-functional analysis reveals that these repressed factors are required for mitotic stability and provide a novel molecular explanation for the conditional lethality observed between BRCA1 and chromosome segregation genes...

Centrosome maturation: measurement of microtubule nucleation throughout the cell cycle by using GFP-tagged EB1

..These results demonstrate that adenovirus infection induces a significant stabilizing effect on host cell microtubule dynamics, which involve, but are not limited to, the activation of the RhoGTPases RhoA and Rac1...

..Recent work in the fission yeast describes a novel mechanism for microtubule self-organization in the absence of centrosomes; this mechanism may function in a variety of cell types found in diverse organisms...

..Mitotic microtubules are moved toward spindle poles in a process known as flux. Several proteins responsible for flux have recently been identified, significantly advancing our understanding of chromosome movements in mitosis...

Stathmin/oncoprotein 18, a microtubule regulatory protein, is required for survival of both normal and cancer cell lines lacking the tumor suppressor, p53

..Our results demonstrate that stathmin is required for cell survival in cells lacking p53, suggesting that stathmin depletion could be used therapeutically to induce apoptosis in tumors without functional p53...