NuPAGE® Tris-Acetate Gels

NuPAGE® Tris-Acetate gels are precast polyacrylamide gels designed to give optimal separation of large molecular weight proteins during gel electrophoresis when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Tris-Acetate gels can also be run with Novex® Tris-Glycine Native Running Buffer to resolve native proteins more effectively than the Tris-Glycine gel system. In comparison to traditional Tris-Glycine SDS-PAGE gels, NuPAGE® Tris-Acetate gels have a pH 8.1 environment that minimizes protein modifications and results in sharper bands.

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Getting started with NuPAGE® Tris-Acetate gels

Get optimal separation of your proteins by choosing the right combination of gel and running buffer. Here is brief summary of the available configurations. See “Choose the right NuPAGE® Tris-Acetate gel” above to get the specific product ordering information.

Polyacrylamide concentrations
NuPAGE® Tris-Acetate gels are available in 2 different polyacrylamide concentrations: 7% and 3–8% gradient. We also offer 8% gels in the midi gel format.

Gel sizes
NuPAGE® Tris-Acetate gels are available in different two sizes: mini gels (10 cm x 10 cm) and midi gels (8.7 cm x 13.3 cm). Both mini gels and midi gels are available with 1.0 mm gel thickness but the 1.5 mm gel thickness is available only with the mini gel format. NuPAGE® Tris-Acetate gels also come in multiple-well formats. For loading volumes, see Recommended Well Loading Volumes & Sample Loads.