CloneEZ® PCR Cloning Kit

GenScript's proprietary CloneEZ® Seamless cloning technology is a cutting-edge, recombination-based cloning technology that can accurately and efficiently assemble genes and gene fragments in any combination of sequences. The CloneEZ® PCR Cloning Kit is designed for the quick cloning of PCR products without any headaches of restriction sites. The universal CloneEZ® cloning method works with any insert and any vector at any restriction site. Using our proprietary CloneEZ® enzyme, this kit rapidly generates precise, directional constructs in 30-min at a room temperature.

What are the differences between the CloneEZ® kit and the PCR Cloning Kit?A: CloneEZ® Seamless cloning technology offers high efficiency, quick
and direct cloning, bypassing all the tedious procedures of traditional
cloning that often involve restriction, ligation, and sometimes
phosphorylation. This technology works with any DNA sequence and
any vector. The only requirement is that your PCR primers have to
have a sequence containing 15 or more nucleotides homologous to vector
sequence.

What factors need to be considered when designing the primer?A: Two sets of primers are used to amplify the gene of interest:1) 15-bp homology regions to the vector, flanking the cloning site into
the insert.2) Specific gene sequence. The start of the 15 bp homology must begin from the 5' most extension to include the overhang, if the
sequence has 5' overhang; if the sequence has a 3' overhang, homology should begin where the DNA becomes double-stranded. Please refer to the manual for illustration of primer design.
low as 20%-30%, and it will lead to a direct repeat deletion.

Can multiple fragments be cloned into a single vector using this kit?
A: Yes, CloneEZ® PCR Cloning Kit can be used for multiple fragments recombination, if there are no many repeated sequences among multiple
fragments. But we still recommend to recombinate one by one after several performances of recombining 2 and 3 fragments, the efficient is
low as 20%-30%, and it will lead to a direct repeat deletion.