Recombinant Human GFR alpha-like His-tag Protein, CF Summary

Measured by its binding ability in a functional ELISA. When Recombinant Human GFR alpha ‑like is coated
at 0.1 μg/mL, 100 μL/well, it binds to Recombinant GDF-15
(Catalog #
9279-GD)
with an ED50 of 0.15-0.9 ng/mL.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GFR alpha-like

GFR
alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family
of proteins (1). Mature human GFR alpha-like is a 376 amino acid (aa)
type I transmembrane protein. It contains a 333 aa extracellular domain, a
20 aa transmembrane domain and a 23 aa cytoplasmic domain. Over the
extracellular domain, human GFRAL shares 72% and 71% identity with mouse and rat GFRAL
respectively. GFRAL is expressed on both
fetal and adult hindbrain neurons of the CNS (3), and would appear to function
as an anti-apoptotic molecule during neuronal stress. GFRAL is a functional receptor for GDF-15,
facilitating weight-loss functions of the protein through c-Ret downstream
signaling. GFRAL
and GDF-15 signaling is implicated in diet-based obesity and insulin resistance
(2-4).