Team:Unesp Brazil

Hope is a robust framework to engineer living therapeutics to treat metabolic disorders. Going from design, fine-tuned expression, biocontainment to real-world application, Hope offers an easy, modular, robust and open-source solution to engineer probiotics and validate them, bringing the next generation of therapeutics one step closer.

Sense and control

Fine-tuned and orthogonal regulatory circuit engineered to compute a specific input and control the expression of the gene of interest. The device is modular, making it easy to reengineer for different applications.

Production

Standardized device to drive production and delivery of therapeutic proteins in the gut. A signal peptide addresses the therapeutic protein to the gut environment, and a cell penetrating peptide drives it through the intestinal epithelium.

Gut simulation

As a proof of concept for our framework, we use Hope to build an alternative treatment for type 1 diabetes. Therefore, we genetically engineered Bacillus subtilis to activate gene expression upon glucose/fructose induction, and produce and secrete a single-chain insulin analog that can be absorbed by intestinal cells. Come with us to learn all about our work!

Hope is a robust framework to engineer living therapeutics to treat metabolic disorders. Going from design, fine-tuned expression, biocontainment to real-world application, Hope offers an easy, modular, robust and open-source solution to engineer probiotics and validate them, bringing the next generation of therapeutics one step closer.

Sense and control

Fine-tuned and orthogonal regulatory circuit engineered to compute a specific input and control the expression of the gene of interest. The device is modular, making it easy to reengineer for different applications.

Production

Standardized device to drive production and delivery of therapeutic proteins in the gut. A signal peptide addresses the therapeutic protein to the gut environment, and a cell penetrating peptide drives it through the intestinal epithelium.

Gut simulation

As a proof of concept for our framework, we use Hope to build an alternative treatment for type 1 diabetes. Therefore, we genetically engineered Bacillus subtilis to activate gene expression upon glucose/fructose induction, and produce and secrete a single-chain insulin analog that can be absorbed by intestinal cells. Come with us to learn all about our work!