The proliferation of BaF3 cells treated with increasing concentrations of mIL-3 was assessed. After 48 hour treatment with mIL-3, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.

The proliferation of MC/9 cells treated with increasing concentrations of mIL-3 was assessed. After 72 hour treatment with mIL-3, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.

Gallery: Mouse Interleukin-3 (mIL-3) #8923

The purity of recombinant mIL-3 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mIL-3 and staining overnight with Coomassie Blue.

The proliferation of BaF3 cells treated with increasing concentrations of mIL-3 was assessed. After 48 hour treatment with mIL-3, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.

The proliferation of MC/9 cells treated with increasing concentrations of mIL-3 was assessed. After 72 hour treatment with mIL-3, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.

Source / Purification

Product Description

Purity:

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-3. All lots are greater than 98% pure.

Molecular Formula:

Recombinant mIL-3 does not have a Met on the amino terminus and has a calculated MW of 15,674. DTT-reduced protein migrates as a 16 kDa polypeptide and non-reduced protein migrates as a 14 kDa polypeptide due to intramolecular cystines. The expected amino-terminal ASISG of recombinant mIL-3 was verified by amino acid sequencing.

Bioactivity:

The bioactivity of recombinant mIL-3 was determined in MC/9 and BaF3 cell proliferation assays. The ED50 of each lot is between 2-50 pg/ml in MC/9 cells and 20-90 pg/mL in BaF3 cells.

Product Usage Information

Storage:
Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

Background

IL-3 is produced by T cells, mast cells and eosinophils (1). Target cells include hematopoietic progenitors, neutrophils, macrophages, mast cells, eosinophils, lymphoid and erythroid cells (1). IL-3 supports growth and differentiation and is used as a media additive to support culture of many cell types (1). The IL-3 receptor is a heterodimer of the IL-3 specific α-chain and the common β-chain, βc, which is also used by GM-CSF and IL-5. (1). Binding of IL-3 can also involve substitution of the βc by a βIL-3-chain that appears to be specific for IL-3 (1,2). Binding of IL-3 to its cognate receptor(s) induces activation of Jak2, phosphorylation of multiple Stats (1,3,5,6), and the PI3K/Akt pathway (1). IL-3 may play an important role in the development of airway inflammation associated with asthma (3,4,5).