Stéphanie Yuki Kolbeck Hotta. 15 ECTS ISA (individual student activity). ”Investigation of transdermal penetration using CARS microscopy”. Diffusion and transport of water in cells and tissue is a vital part of all life. However directly visualizing water in cell and tissue is not possible using traditional microscopy techniques. In the current study Coherent anti Stokes Raman scattering (CARS) microscopy was used to investigate the diffusion of D2O through human skin. 2016. Supervisor Jonathan Brewer.

Marie Karen Tracy Hong Lin. 10 ECTS ISA (individual student activity). ”Bioimaging and lipid analysis of human epidermis and cholesteatoma”. Acquired cholesteatoma is characterized by uncontrolled growth of keratinizing squamous epithelium in the middle ear. Studies showed difference in the lipid composition between the human skin and acquired cholesteatoma. High Performance Liquid Chromatography (HPLC) and mass spectrometry were thus used to measure and identify the lipid composition in acquired cholesteatoma for comparison against the human epidermis. Confocal fluorescence microscopy was used to visualize the structures after labeling specific proteins using immunocytochemistry techniques. 2016. Supervisor Jonathan R. Brewer.

Marie Karen Tracy Hong Lin. 15 ECTS ISA (individual student activity). ”CARS imaging of glucose uptake in the arterial wall”. Diabetes, obesity and dyslipidemia are all shown to be characterized by endothelial dysfunction of the arterial wall. To develop a protocol for imaging the glucose uptake in arterial wall, Coherent anti-Strokes Raman (CARS) microscopy was used to visualize d7-glucose uptake in the wall of resistance arteries. d7-glucose was used to differentiate between glucose already in the sample and glucose added. 2016. Supervisor Jonathan R. Brewer.

Brian Bjarke Jensen. 10 ECTS ISA (individual student activity). "Diver" project". Implementation of New Methods for Deep Tissue Imaging. To be able to "look" deeper inside tissue, a new method has been developed. This method is a combination of Fluorescence-lifetime imaging microscopy (FLIM) and Two-photon laser scanning microscope and is known as deep tissue imaging microscopy (DIVER) and takes into account the intense scattering of light that is produced by the tissue. This new method claims to be able to visualize structures as deep as 3 mm inside of a sample. 2016. Supervisor Jonathan R. Brewer.