Received October 1, 2017; Revised October 28, 2017; Accepted October 30, 2017.

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Abstract

In March 2016, an isolate of Cucumber mosaic virus (named Gyp-CMV) was isolated from the Sambungai (Gynura procumbens) showing the symptoms of mosaic and chlorosis. The isolate Gyp-CMV was characterized by disease reactions in several indicator plants, reverse transcription-polymerase chain reaction (RT-PCR), PCR-restriction fragment length polymorphism, and sequence analysis of movement protein (3a) and coat protein (CP) genes. Tobacco, tomato, pepper, ground cherry, and lambsquarters (Chenopodium quinoa and C. amaranticolor) appeared typical CMV symptoms, but zucchini and cucumber were not infected. Phylogenetic analysis of the 3a and CP gene indicated that Gyp-CMV belongs to the CMV subgroup II. Sequence identities of the Gyp-CMV 3a and CP genes showed 99.3% and 100% to that of Hnt-CMV at amino acid level. To our knowledge, this is the first report of CMV infection in Gynura procumbens.

This study was carried out with the support of Cooperative Research Program for Agricultural Science & Technology Development (Project No. PJ012266012017 and PJ010878042017) in Rural Development Administration, Republic of Korea, basic science Research Program through the NRF funded by the Ministry of Education (NRF-2017R1D1A1B03036553) and 2015 Research Grant from Kangwon National University.

Conflicts of Interest

The authors declare that they have no competing and commercial interests in this work.