Product Description

The Rab family of GTPases regulates eukaryotic vesicular membrane traffic. Rab35 is with both plasma membrane and endosomal localization, and has been implicated in diverse processes that include T-cell receptor recycling, oocyte yolk protein recycling and cytokinesis. Rab35 regulates neurite outgrowth in neuronal-like cells, and can induce protrusions.

Currently there is no direct assay to measure the activation of Rab35 GTPases.

NewEast Biosciences Rab35 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab35-GTP, but not Rab Rab35-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.

These anti- Rab35-GTP monoclonal antibodies can also be used to monitor the activation of Rab35 in cells and in tissues by immunohistochemistry.