UTHSC VIRAL VECTOR CORE LABORATORY

UTHSC Viral Vector Core Laboratory (VVC) was established with support from a Department
of Defense grant awarded in May 2007. Located in the Cancer Research Building on
the Memphis campus of UTHSC, the VVC provides services to the Memphis, Knoxville
and Chattanooga campuses. Viral Vector Core services include lentiviral, adenoviral
and adeno-associated viral vector, as well as traditional retroviral vectors. Vectors
services are available to all UTHSC investigators to support their research efforts
on a fee-for-service basis.

The most advanced Lentiviral vectors with capacity to deliver 7 kbp of your gene
sequence.
Lentiviral vectors are derived by deletion of the replication genes from human HIV,
a member of the Retroviridae virus family. The deletions make the vectors safe and provide space for gene inserts.

Lentiviral vectors from the Vector Core offer:

Extraordinarily efficient gene delivery – 100% of cells are infected in most
cases.

Infection of all mammalian species and cell types using the Vesicular Stomatitis
Virus glycoprotein (VSV-G) to coat the vectors.

The most advanced Adenoviral vectors with capacity to deliver up to 7 kbp of your
gene sequence.
Adenoviral vectors are derived from human adenovirus serotype 5 by deletion of the
early gene E1 and E3 regions. The gene of interest is usually inserted in the E1
region of adeno-genome.

Adenoviral vectors from the Vector Core offer:

Extraordinarily efficient gene delivery – 100% of cells are infected in most
cases.

Infection of many types of human and rodent cells and many cell types in vivo.

Gene delivery to non-dividing and dividing cells.

Very high levels of gene expression that can last for three to six months.

Regulated and constitutive overexpression of your gene.

Create gene knock-downs in cell culture using miRNA – these can also be regulated!

State-of-the-art Adeno-associated vectors with capacity to deliver up to 4.5 kbp
of your gene sequence.
Adeno-associated viral vectors are derived from naturally replication deficient parvoviruses,
which have not been associated with any human diseases.This parent parvovirus cannot replicate without a helper virus such as adenovirus
or herpesvirus.

The Viral Vector Core offers a helper-free production system based on human adeno-associated
virus-2 (AAV-2) with the following features: