Suitable for use in ELISA, Western Blot, Immunofluorescence, and Immunohistochemistry. Other applications not tested.

Recommended Dilution

Western Blot:1:50-1:500 (ECL) On denatured Western blots of purified bovine NSE the antibody recognizes a single band at ~39kD. On Western blots of whole rat brain lysates a more complex series of bands is observed ranging from 78/80kD (NSE homodimer) to 20kD (gamma-enolase degradation product).

May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Clone Type

Polyclonal

Isotype

IgG

Host

Rabbit

Concentration

Not determined.

Form

Supplied as a liquid in 0.1% sodium azide, before the addition of glycerol to 40%.

Purity

Serum.

Immunogen

Synthetic peptide from human NSE.

Specificity

Recognizes NSE. Stains many neuronal cell bodies and processes within the central and peripheral nervous system. Species Crossreactivity

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.