DFF45 AntibodyCATALOG NUMBER: 1141

Immunofluorescence of DFF45 in Hela cells with DFF45 antibody at 20 μg/mL.

Green: DFF45 Antibody (1141) Blue: DAPI staining

Immunocytochemistry of DFF45 in HeLa cells with DFF45 antibody at 5 μg/mL.

« Prev123Next »

Product Price Options

*Size

0.02 mg - $79.00

0.1 mg - $335.00

$0.00

Qty:

Specifications

Properties

Additional Info

Background

Specifications

SPECIES REACTIVITY:

Human

TESTED APPLICATIONS:

ELISA, ICC, IF, WB

APPLICATIONS:

DFF45 antibody can be used for detection of DFF45 and one of the cleaved fragments of DFF45 by Western blot at at 0.5 - 1 μg/mL and for immunoprecipitation. A 45 kDa band can be detected in non-apoptotic cells. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

USER NOTE:

Optimal dilutions for each application to be determined by the researcher.

POSITIVE CONTROL:

1) Cat. No. 1201 - HeLa Cell Lysate

2) Cat. No. 17-001 - HeLa Cell Slide

PREDICTED MOLECULAR WEIGHT:

45 kDa

IMMUNOGEN:

DFF45 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DFF45.

The immunogen is located within the last 50 amino acids of DFF45.

HOST SPECIES:

Rabbit

Properties

PURIFICATION:

DFF45 Antibody is Antibody is DEAE purified.

PHYSICAL STATE:

Liquid

BUFFER:

DFF45 Antibody is supplied in PBS containing 0.02% sodium azide.

CONCENTRATION:

batch dependent

STORAGE CONDITIONS:

DFF45 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Background

BACKGROUND:

DFF45 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD. Upon cleavage of DFF45/ICAD, a caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.