This kit is designed for the rapid spin column preparation of plasmid DNA from up to 100 mL of Escherichia coli cultures. The kit allows for the isolation of plasmid DNA with final endotoxin levels of 0.1 EU/µg of DNA or less. The kit is able to purify plasmids up to 13,000 bp in size, and typical yields from a 100 mL culture for a high copy number plasmid are between 0.4 and 1.0 mg. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with manual or automated sequencing to achieve 95-100% accuracy, bacterial transformation, transfections and more.

Kit Specifications

Column Binding Capacity

1.5 mg

Average Yield from 100 mL Culture*

0.4 - 1.0 mg

Final Endotoxin Levels

< 0.1 EU/µg DNA

Time to Complete 4 Purifications

1.5 hours

Size of Plasmids Purified

Up to 13,000 bp

* Depends on high-copy DNA and low copy DNA

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers. The RNAse vial should be stored at -20°C upon arrival. The Resuspension Buffer should be stored at 4oC upon addition of RNAse enzyme.