This study aimed to assess which is the most appropriate protocol for extraction of chlorophylls a, b and total (a + b) to be applied in studies with Tifton 85 (Cynodon spp.) forage grass. Results revealed that, based on total (a + b) chlorophyll contents, the best extractors were N,N-dimethylformamide, dimethylsulfoxide and acetone 80% (using the equations proposed by ARNON, 1949). Temporal changes in the concentrations of chlorophyll a, b and total were well described by a hyperbolic model common to all solvents, being the period of 48 hours considered sufficient for an appropriate extraction.