@article {Schreiberpp.01218.2018,
author = {Schreiber, Tom and Prange, Anja and Hoppe, Tina and Tissier, Alain F},
title = {Split-TALE: A TALE-based two-component system for synthetic biology applications in planta},
elocation-id = {pp.01218.2018},
year = {2019},
doi = {10.1104/pp.18.01218},
publisher = {American Society of Plant Biologists},
abstract = {Transcription activator-like effectors (TALEs) are bacterial Type-III effector proteins from phytopathogenic Xanthomonas species that act as transcription factors in plants. The modular DNA-binding domain of TALEs can be reprogrammed to target nearly any DNA sequence. Here we design and optimize a two-component AND gate system for synthetic circuits in plants based on TALEs. In this system, named split-TALE (sTALE), the TALE DNA binding domain and the transcription activation domain are separated and each fused to protein interacting domains (IDs). Physical interaction of IDs leads to TALE-reconstitution and can be monitored by reporter gene induction. This setup was used for optimization of the sTALE scaffolds, which result in an AND-gate system with an improved signal-to-noise ratio. We also provide a toolkit of ready-to-use vectors and single modules compatible with Golden Gate cloning and MoClo syntax. In addition to its implementation in synthetic regulatory circuits, the sTALE system allows the analysis of protein-protein interactions in planta.},
issn = {0032-0889},
URL = {http://www.plantphysiol.org/content/early/2019/02/12/pp.18.01218},
eprint = {http://www.plantphysiol.org/content/early/2019/02/12/pp.18.01218.full.pdf},
journal = {Plant Physiology}
}