Busulfan degrades quickly at room temperature. Each sample should be kept in wet ice slurry or refrigerated at all time. Centrifuge them as soon as possible at 4 °C. Separate the plasma from each sample and transfer it to individual 4mL plastic tubes. Immediately freeze these plasma samples at -20 °C.

All samples have to be labeled with patient name, Medical Record #, date and actual clock time of the blood draw. Two unique identifiers and clock times are REQUIRED or samples can be rejected.

Samples must be shipped on a minimum of 3 kg of dry ice using an overnight carrier. Samples that arrived thawed will not be analyzed.

The REQUISITION SHEET for particular regimen, IV Q6 hr or test dose, IV Q24 hr, and Oral Q6 hr is provided below. It is required to be sent with the samples. Patient identifiers must be matched with those on the collected samples mentioned above. Also, our requisition serves as physician’s order and must be signed by the attending physician or designee/caregiver according to the College American Pathologists (CAP) requirement.

Results

We usually release pharmacokinetics results on the same day as receipt of the samples. Average sample run-time is approximately 4 to 6 hours from receiving the package.

Sample processing is prioritized by time zone differences or next dose due time to maximize the advantage of dose adjustments.

All results must be verbally delivered to attending physician, pharmacist, or caregiver who is a certified MD or PharmD. No exceptions.

After the verbal report, a hardcopy can be faxed to designated number(s). Please click here for an example of our facsimile report.

CPT Code

The current CPT code for busulfan monitoring is 80299.

New accounts

When setting up a new account with us for busulfan analysis, please have the following information ready and call (206) 606-7389.

Institution information: Name of the institution, address, and general contact numbers.

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Seattle Cancer Care Alliance is proud to be an Equal Opportunity and VEVRAA Employer. We are committed to cultivating a workplace in which diverse perspectives and experiences are welcomed and respected. We do not discriminate on the basis of race, color, religion, creed, ancestry, national origin, sex, age disability, marital or veteran status, sexual orientation, gender identity, political ideology, or membership in any other legally protected class. We are an Affirmative Action employer. We encourage individuals with diverse backgrounds to apply and desire priority referrals of protected veterans. Read EEO is the Law poster here.