Chk1, like the other checkpoint kinase Chk2, is a serine/threonine protein kinase that plays a crucial role in cell cycle control. Chk1 becomes phosphorylated and consequently activated in response to DNA damage by the ATR kinase. Upon this activation Chk1 phosphorylates a variety of proteins including p53 and Cdc25, which triggers cell cycle arrest, DNA repair and apoptosis (Bartek and Lukas, 2003).

Chk1 plays a critical role in preventing aberrant cells from progressing through the cell cycle. Based on this, it is not surprising that Chk1 is of interest in the field of cancer research and as a therapeutic target (Thompson and Eastman, 2013).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species

Cross Reactivity

Mouse

Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN3)50% Glycerol

Immunogen

Recombinant human Chk1

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage

Store at +4oC or at -20oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Applications of Chk1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name

Verified

Min Dilution

Max Dilution

Western Blotting

1 ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.