Bottom Line:
More sensitive and effective diagnostic markers for the detection of breast cancer are urgently needed.The miR-183/182/96 cluster levels were significantly higher in breast cancer tissues than in control tissues.An increased miR-183/182/96 cluster level was correlated with local relapse, distant metastasis and poor clinical outcomes.

ABSTRACTMore sensitive and effective diagnostic markers for the detection of breast cancer are urgently needed. The microRNA-183/182/96 cluster has been reported to be involved in tumorigenesis and progression in a variety of cancers, and it is a promising cancer prognostic biomarker. The goal of this study was to determine the expression levels of the miR-183/182/96 cluster in breast cancer tissues and evaluate its prognostic role in breast cancer. Real-time quantitative polymerase chain reaction analysis (qRT-PCR) was used to detect the expression levels of the miR-183/182/96 cluster in 41 breast cancer tissues and adjacent normal tissues (control tissues) and also in different mammary cell lines. In situ hybridization (ISH) of the miR-183/182/96 cluster on 131 tissue microarrays (TMAs) was used to statistically analyze its prognostic role. The miR-183/182/96 cluster levels were significantly higher in breast cancer tissues than in control tissues. The miR-183/182/96 cluster was also upregulated in human breast cancer cell lines. An increased miR-183/182/96 cluster level was correlated with local relapse, distant metastasis and poor clinical outcomes. Our findings improve our understanding of the expression level of the miR-183/182/96 cluster in breast cancer and clarify the role of the miR-183/182/96 cluster as a novel prognostic biomarker for breast cancer.

f3: Increased miR-183/182/96 level was correlated with poor prognosis.(A) OS curves for 131 patients with low or high miR-183, miR-182, miR-96 and miR-183/182/96 cluster expression. Kaplan-Meier and log-rank analyses were used. High levels of the miR-183/182/96 cluster were markedly correlated with shorter overall survival. (B) DFS curves for 131 patients with low or high miR-183, miR-182, miR-96 and miR-183/182/96 cluster expression. A high level of the miR-183/182/96 cluster was markedly correlated with shorter disease-free survival. All data are shown as the mean ± SE ***P < 0.01.

Mentions:
The results above revealed that miR-183/182/96 level was higher in breast cancer and was positively correlated with some clinical pathological parameters, indicating that miR-183/182/96 may be vital for the pathogenesis of breast cancer. To further explore the prognostic significance of miR-183/182/96, we used Kaplan-Meier survival analysis to generate patient overall survival (OS) and disease-free survival (DFS) curves. The results demonstrated that patients with higher miR-183, miR-182 and miR-96 expression levels had shorter mean OS and DFS than patients with lower expression levels (P = 0.000, 0.000 and 0.022 for OS, respectively and P = 0.000, 0.004 and 0.011 for DFS, respectively, Fig. 3A,B). Further more, when the expression levels of all three members of the miR-183/182/96 cluster were high, OS and DFS were much shorter than when zero, one, or two members of the miR-183/182/96 cluster were highly expressed (all P for OS = 0.000 and all P for DFS = 0.004). These results demonstrated that miR-183/182/96 expression was significantly associated with patient OS and DFS. The miR-183/182/96 cluster is a potential prognostic biomarker for breast cancer.

f3: Increased miR-183/182/96 level was correlated with poor prognosis.(A) OS curves for 131 patients with low or high miR-183, miR-182, miR-96 and miR-183/182/96 cluster expression. Kaplan-Meier and log-rank analyses were used. High levels of the miR-183/182/96 cluster were markedly correlated with shorter overall survival. (B) DFS curves for 131 patients with low or high miR-183, miR-182, miR-96 and miR-183/182/96 cluster expression. A high level of the miR-183/182/96 cluster was markedly correlated with shorter disease-free survival. All data are shown as the mean ± SE ***P < 0.01.

Mentions:
The results above revealed that miR-183/182/96 level was higher in breast cancer and was positively correlated with some clinical pathological parameters, indicating that miR-183/182/96 may be vital for the pathogenesis of breast cancer. To further explore the prognostic significance of miR-183/182/96, we used Kaplan-Meier survival analysis to generate patient overall survival (OS) and disease-free survival (DFS) curves. The results demonstrated that patients with higher miR-183, miR-182 and miR-96 expression levels had shorter mean OS and DFS than patients with lower expression levels (P = 0.000, 0.000 and 0.022 for OS, respectively and P = 0.000, 0.004 and 0.011 for DFS, respectively, Fig. 3A,B). Further more, when the expression levels of all three members of the miR-183/182/96 cluster were high, OS and DFS were much shorter than when zero, one, or two members of the miR-183/182/96 cluster were highly expressed (all P for OS = 0.000 and all P for DFS = 0.004). These results demonstrated that miR-183/182/96 expression was significantly associated with patient OS and DFS. The miR-183/182/96 cluster is a potential prognostic biomarker for breast cancer.

Bottom Line:
More sensitive and effective diagnostic markers for the detection of breast cancer are urgently needed.The miR-183/182/96 cluster levels were significantly higher in breast cancer tissues than in control tissues.An increased miR-183/182/96 cluster level was correlated with local relapse, distant metastasis and poor clinical outcomes.

ABSTRACTMore sensitive and effective diagnostic markers for the detection of breast cancer are urgently needed. The microRNA-183/182/96 cluster has been reported to be involved in tumorigenesis and progression in a variety of cancers, and it is a promising cancer prognostic biomarker. The goal of this study was to determine the expression levels of the miR-183/182/96 cluster in breast cancer tissues and evaluate its prognostic role in breast cancer. Real-time quantitative polymerase chain reaction analysis (qRT-PCR) was used to detect the expression levels of the miR-183/182/96 cluster in 41 breast cancer tissues and adjacent normal tissues (control tissues) and also in different mammary cell lines. In situ hybridization (ISH) of the miR-183/182/96 cluster on 131 tissue microarrays (TMAs) was used to statistically analyze its prognostic role. The miR-183/182/96 cluster levels were significantly higher in breast cancer tissues than in control tissues. The miR-183/182/96 cluster was also upregulated in human breast cancer cell lines. An increased miR-183/182/96 cluster level was correlated with local relapse, distant metastasis and poor clinical outcomes. Our findings improve our understanding of the expression level of the miR-183/182/96 cluster in breast cancer and clarify the role of the miR-183/182/96 cluster as a novel prognostic biomarker for breast cancer.