Conditional Knockout

C57BL/6 Background

The targeting vector has been generated using C57BL/6J DNA and transfected into TaconicArtemis C57BL/6NTac ES cell line.

Exon 2 contains the translation initiation codon.

Exons 6 to 11 have been flanked by loxP sites (size of loxP-flanked region: 4.0 kb).

The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.

The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.

The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 6 to 11 should result in the loss of function of the Fgfr4 gene by deleting half of the second Ig-like C2-type domain, the complete third Ig-like C2-type domain and the transmembrane domain, and by generating a frameshift from exon 5 to exons 12 and 13 (premature Stop codon in exon 12).