Yang YangDepartment of Dairy and Animal Science, The Center for Reproductive Biology and Health, College of Agricultural Sciences, The Pennsylvania State University, University Park, PA 16802, USABMC Genomics 12:13. 2011

..and the identification of mutations in the coding sequence of bovine myostatin in two breeds of double-muscled cattle, Belgian Blue and Piedmontese, which are known to have an increase in muscle mass relative to conventional cattle...

Genetic and functional confirmation of the causality of the DGAT1 K232A quantitative trait nucleotide in affecting milk yield and composition

..used a positional cloning approach to identify a nonconservative lysine to alanine substitution (K232A) in the bovine DGAT1 gene that was proposed to be the causative quantitative trait nucleotide underlying a quantitative trait ..

Detection of haplotypes associated with prenatal death in dairy cattle and identification of deleterious mutations in GART, SHBG and SLC37A2

..Since this is the first finding of stop codon readthrough in a common mammalian protein, detailed analysis of L-MPZ expression will help to understand the mechanism of stop codon readthrough in mammals...

Phosphorylation and activation of myosin by Rho-associated kinase (Rho-kinase)

Yang YangDepartment of Dairy and Animal Science, The Center for Reproductive Biology and Health, College of Agricultural Sciences, The Pennsylvania State University, University Park, PA 16802, USABMC Genomics 12:13. 2011

..These transpositions are lineage-dependent. The purpose of this study is to investigate the lineage-specific Y-chromosome genes in bovid...

..BWT), preweaning average daily gain (PWADG), and average daily gain on feed (ADGF) in commercial lines of Bos taurus. The QTL regions for the growth traits identified using a haplotype association analysis, which included the ..

Bovine TLR2 and TLR4 properly transduce signals from Staphylococcus aureus and E. coli, but S. aureus fails to both activate NF-kappaB in mammary epithelial cells and to quickly induce TNFalpha and interleukin-8 (CXCL8) expression in the udder

..of electrostatic charges, which then appears to be the principal structural feature of the "healthy" PrP form that might affect the stringency of the species barrier for transmission of prion diseases between humans and cattle.

b>Bovine IF(1), a basic protein of 84 amino acids, is involved in the regulation of the catalytic activity of the F(1) domain of ATP synthase. At pH 6.5, but not at basic pH values, it inhibits the ATP hydrolase activity of the enzyme...

..Identification of a set of residues that mediate interactions between the transmembrane helices and the cytoplasmic surface, where G-protein activation occurs, also suggests a possible structural change upon photoactivation...

Copy number variation of testis-specific protein, Y-encoded (TSPY) in 14 different breeds of cattle (Bos taurus)

..NPC2 has an Ig-like fold stabilized by three disulfide bonds. The structure of the bovine protein reveals a loosely packed region penetrating from the surface into the hydrophobic core that forms adjacent ..

Mapping of a milk production quantitative trait locus to a 420-kb region on bovine chromosome 6

We have determined the primary structure of a myosin I (called mammalian myosin I beta, MMI beta) from bovine brain and identified its functional domains. The protein was previously purified from brain and adrenal gland...

Closing the folding chamber of the eukaryotic chaperonin requires the transition state of ATP hydrolysis

..Our results indicate that lactate suppresses lipolysis in adipose tissue through a direct activation of GPR81. GPR81 may thus be an attractive target for the treatment of dyslipidemia and other metabolic disorders...

Uroplakin Ia is the urothelial receptor for uropathogenic Escherichia coli: evidence from in vitro FimH binding

G ZhouSkirball Institute of Biomolecular Medicine, Department of Biochemistry, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USAJ Cell Sci 114:4095-103. 2001

..coli, and that the binding of uropathogenic bacteria to uroplakin Ia may play a key role in mediating the urothelial responses to bacterial attachment...

Three-dimensional structure of the ATPase fragment of a 70K heat-shock cognate protein

The three-dimensional structure of the amino-terminal 44K ATPase fragment of the 70K bovine heat-shock cognate protein has been solved to a resolution of 2.2 A...

Uroplakins Ia and Ib, two major differentiation products of bladder epithelium, belong to a family of four transmembrane domain (4TM) proteins

J YuRonald O Perelman Department of Dermatology, New York University Medical School, New York 10016J Cell Biol 125:171-82. 1994

..indicate that the outer leaflet of AUM is composed of crystalline patches of 12-nm protein particles, and that bovine AUMs contain three major proteins: the 27- to 28-kD uroplakin I, the 15-kD uroplakin II and the 47-kD uroplakin ..

X R WuRonald O Perelman Department of Dermatology, New York University School of Medicine, NY 10016J Cell Sci 106:31-43. 1993

..The fact that of the three major proteins of AUM only UPIII has a significant cytoplasmic domain suggests that this molecule may play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells...

..the impact of this system on the counteraction of infections of the mammary gland (mastitis), we characterized the bovine gene encoding the key PRR Toll-like receptor 9 (TLR9) and mapped its precise position on chromosome BTA22...

After ovulation in the cow, the corpus luteum (CL) rapidly develops within a few days with angiogenesis and progesterone production. CL formation resembles an inflammatory response due to the influx of immune cells...

..In addition, HMWCaMBP also showed the same affinities for m-calpain as calpastatin I and calpastatin II. Our findings suggest that HMWCaMBP is the homolog of calpastatin and may be a CaM-binding form of calpastatin...

The acute-phase protein serum amyloid A3 is expressed in the bovine mammary gland and plays a role in host defence

..study was to characterize the mRNA expression of different ASIP transcripts and of putative targets in different bovine tissues, as well as to study consequences on protein abundance and localization...

..The consistency and magnitude of the increase in ANGPTL4 mRNA across multiple models of altered energy balance identifies it as an adipokine that is uniquely responsive to changes in energy balance in the lactating dairy cow.

Adiponectin deficit during the precarious glucose economy of early lactation in dairy cows

..Our findings indicate that p160ROCK-mediated Ca2+ sensitization is involved in the pathophysiology of hypertension and suggest that compounds that inhibit this process might be useful therapeutically...

Short communication: Effects of the progesterone receptor variants on fertility traits in cattle

..Currently, it is unknown what variants of the PGR gene are related to fertility traits in cattle. Identification of such variants would allow the implementation of marker-assisted selection in breeding schemes...

Calpain 1-titin interactions concentrate calpain 1 in the Z-band edges and in the N2-line region within the skeletal myofibril

..Furthermore, calpain 1 is found to localize in myofibril fractures, described as proteolysis sites, in postmortem bovine skeletal red muscles, near the calcium deposits located at the N1 and N2 level...

Identification and prevalence of a genetic defect that causes leukocyte adhesion deficiency in Holstein cattle

..We find, surprisingly, that the channel contains three CNGA1 subunits and only one CNGB1 subunit. These results have implications for CNG channel function in particular and assembly of membrane proteins in general...

Polymorphism in genes of growth hormone receptor (GHR) and insulin-like growth factor-1 (IGF1) and its association with both the IGF1 expression in liver and its level in blood in Polish Holstein-Friesian cattle

Polymorphisms in the bovine ghr and igf1 genes. Ghr and igf1 genes have been associated with milk and meat production of cattle. However, the molecular and physiological mechanisms underlying such associations are unknown...

A small set of extra-embryonic genes defines a new landmark for bovine embryo staging

..in MEC the role of NF-κB and C/EBP factors in regulating basal and pathogen-induced expression of both genes from cattle. Abrogating NF-κB binding to that site by introduction of a single point mutation blocks promoter activity of ..

The structure of bovine F(1)-ATPase inhibited by a monomeric form of the inhibitor protein, IF(1), known as I1-60His, lacking most of the dimerization region, has been determined at 2.1-A resolution...

Novel mutations of the FASN gene and their effect on fatty acid composition in Japanese Black beef

The major protein of bovine seminal plasma, PDC-109, binds to choline phospholipids on the sperm plasma membrane and induces the efflux of cholesterol and choline phospholipids, which is an important step in sperm capacitation...

Historically, dwarfism was the major genetic defect in U.S. beef cattle. Aggressive culling and sire testing were used to minimize its prevalence; however, neither of these practices can eliminate a recessive genetic defect...

Research Grants78

..Transplacental transmission of neospora caninum results in abortions, stillbirths, and congenital infection in cattle and dogs. This apicomplexan parasite is similar to Toxoplasma gondii and Plasmodium sp...

..typhimurium variants (phage types DT2 and DT99) that cause fatal pigeon paratyphoid do not persist in cattle. The objective of this proposal is to identify the genes responsible for the epidemiologic success of S...

DESCRIPTION (provided by applicant): Bovine ovarian superovulation is the current state-of-the-art method employed worldwide to maintain and improve milk and beef production through genetic selection of the best quality breeders and best ..

F Nina Papavasiliou; Fiscal Year: 2014

..The parasite Trypanosoma brucei is the causative agent of African sleeping sickness in humans and of Nagana in cattle, and one of the leading impediments to development in much of sub-Saharan Africa. T...

Mingming Wu; Fiscal Year: 2014

..For this project, the bovine model will not only provide useful information to the cattle industry but also to human medicine, because ..

..hepatica causes liver fluke disease or fascioliasis, thereby affecting the health of humans, as well as sheep, cattle and goats, among others mammalians. Fascioliasis causes losses in agriculture estimated at >US3...

Pablo Juan Ross; Fiscal Year: 2016

..Early embryonic mortality is a major cause of infertility in dairy cattle and humans. In bovine embryos, methylation of histone H3 lysine 27 is one of the epigenetic marks that are dynamically remodeled...

Christina Sigurdson; Fiscal Year: 2016

..Zoonotic prion transmission is of concern, as the bovine spongiform encephalopathy (BSE) epidemic has led to nearly 200 cases of prion infection in humans...

Se Jin Lee; Fiscal Year: 2014

..Subsequent genetic studies in cattle, sheep, dogs, and humans have all shown that the function of myostatin as a negative regulator of muscle mass has ..

..studies proposed here will establish a new paradigm for the genetic modification of artiodactyls (pigs, sheep, and cattle) for use as large animal models of human disease and the production of biomedical materials...

Claudio Soto; Fiscal Year: 2016

..Although rare, the recent outbreak of Bovine Spongiform Encephalopathy(BSE) in cattle and Chronic Wasting disease (CWD) in cervids and the transmission of the ..

..TSEs include scrapie in sheep, bovine spongiform encephalopathy (BSE) in cattle, chronic wasting disease (CWD) in deer and elk, and several forms of ..

Lisa Murphy; Fiscal Year: 2016

..If the Charm ROSA-M readers can be validated for the analysis of dog food, cattle feed, and swine feed, they will provide an easy method for minimally-trained personnel at multiple laboratories to ..

Claudio Soto; Fiscal Year: 2014

..sCJD), the most common form, and variant CJD (vCJD) which is associated to consumption of cattle meat infected by bovine spongiform encephalopathy...

James D Bangs; Fiscal Year: 2015

..are parasitic protozoa that cause human African trypanosomiasis (HAT, sleeping sickness), as well as nagana in cattle and other livestock...

GREGORY CHARLES GRAY; Fiscal Year: 2016

..Mongolia has undergone rapid change with increases in mining operations and animal production of sheep, goats, and cattle. Large segments of the normally nomadic pastoral populations have begun migrating to urban areas and introducing ..

Julia K Gilden; Fiscal Year: 2015

..protozoa responsible for causing human African trypanosomiasis (HAT, sleeping sickness) in humans and nagana in cattle and other livestock...

Peng George Wang; Fiscal Year: 2016

..coli fermentation. Such a bioconjugate already offers good possibility to be used to induce antibodies in cows or cattle to kill an inoculum of E. coli O157, since the vaccines can be produced in large scale economically...

..Importantly, protective immunity against Ov larvae has now been definitively demonstrated in humans, cattle and mice, thereby proving the conceptual underpinnings that a vaccine can be produced against this infection...

Shinji Makino; Fiscal Year: 2014

..disease of sub-Saharan Africa that can emerge in explosive mosquito-borne epidemics, decimating herds of sheep and cattle and resulting in enormous economic losses;humans are infected concurrently, and their infection results in ..

Kirk E Smith; Fiscal Year: 2014

..specific questions about well water and other exposures), and application of GIS using data sources such as cattle density, geologic sensitivity to groundwater contamination, and climate variables (e.g., flooding)...

Philip C Hopewell; Fiscal Year: 2014

..livestock keepers (pastoralists) combined with poor public health infrastructure, limited TB control measures for cattle and animal products, and a large immunocompromised human population due to HIV/AIDS, make Africa particularly ..

Robert Sheridan; Fiscal Year: 2014

..If funded we propose to expand the inspection and surveillance testing of cattle feeds and feed ingredients manufactured and distributed in NY State...

CLAUDIA G COLES; Fiscal Year: 2014

..WSDA describes Washington State's vulnerability to BSE agent release from imported Canadian cattle in age cohorts shared by the ongoing BSE positives identified by Canadian BSE surveillance...

..A causative gene, LIMBIN for bovine chondrodysplastic dwarfism (bcd) found in Japanese brown cattle was later identified as the bovine ortholog of EVC2, indicating the significance of EVC2 protein function...

..plan to study three strains of prions passaged in mice: RML and 301V, which originated from sheep with scrapie and cattle with BSE, respectively, plus a novel synthetic strain MoSP1, which is the most stable strain reported to date...

..be transmitted to humans directly (Aim 1)? Can CWD be transmitted to humans after passage through secondary hosts (cattle or sheep) (Aim 2)? Has CWD transmission to humans already occurred (Aim 3)? The ultimate goals are to define the ..

..disease, fatal familial insomnia and Kuru in humans, scrapie in sheep, bovine spongiform encephalopathy in cattle, and chronic wasting disease in deer and elk. While rare, these diseases are always fatal...

..agents of African trypanosomiasis or sleeping sickness in humans and a wasting and fatal disease (Nagana) in cattle, domestic pigs and other farm animals causing a profound effect on the economy of much of the continent...

..The World Health Organization lists the groups of people at risk of the infection ranging from cattle and pig farmers to veterinarians, butchers and slaughterhouse workers to children playing in rain puddles or mud, ..

..The overall HYPOTHESIS to be evaluated is that the consumption of beef products, derived from Z-implanted beef cattle may possess a potential health impact on human consumers, particularly with respect to reproductive endocrinology ..

..The incidence of immune-mediated abortion and placental insufficiency in both humans and cattle are greatly increased with the use of assisted reproductive technologies such as in vitro fertilization...

..Despite these findings, however, the genes that contribute to milk production are largely unidentified. In the bovine, there are quantitative trait loci (QTL) for milk volume present on all 29 autosomes...

Patents62

SPECIFIC SNP COMPOSITION IN HANWOO USING NGS AND IDENTIFICATION METHOD BY USING THE SAME