Hierarchical cluster analysis of genes and experiments based on cDNAs identified as
being significantly different in expression between normal genital skin fibroblasts
and genital skin fibroblasts of female patients with AIS. The left panel shows an
overview of 472 of the total of 487 significant transcripts that showed measurable
expression across at least 80% of 24 ex-periments. The color code of the dendrogram
and the sample names represent the origin of the fibroblast strains. The scale ranges
from -4 to +4 in log2 space. (Complete dataset for Figure 2.)

Hierarchical cluster analysis of genes and experiments with different DHT treatment
regimens. Shown are the 2862 transcripts that distinguish between normal genital skin
fibroblasts and gonadal fibroblasts from 46, XY female AIS patients, and between proliferating
and confluent fibroblasts. The color code in the dendrogram depicts the origin of
the fibroblast cultures. The gray and white bars on top of the cluster indicate the
proliferation state of the samples. On the right, the regions of the cluster diagram
are indicated which differentiate between normal and AIS-derived fibroblasts, and
proliferating and confluent cells, respectively. No differences in transcript levels
could be discerned between DHT treated and control cells in either normal foreskin
fibroblasts or fibroblasts from AIS affected 46, XY females. The scale ranges from
-8 to +8 in log2 space. (Complete dataset for Figure 3.)

Upper half of Figure. Hierarchical cluster analysis of genes and experiments with
different DHT treatment regimens. Shown are the 2862 transcripts that distinguish
between normal genital skin fibroblasts and gonadal fibroblasts from 46, XY female
AIS patients, and between proliferating and confluent fibroblasts. The color code
in the dendrogram depicts the origin of the fibroblast cultures. The gray and white
bars on top of the cluster indicate the proliferation state of the samples. On the
right, the regions of the cluster diagram are indicated which differentiate between
normal and AIS-derived fibroblasts, and proliferating and confluent cells, respectively.
No differences in transcript levels could be discerned between DHT treated and control
cells in either normal foreskin fibroblasts or fibroblasts from AIS affected 46, XY
females. The scale ranges from -8 to +8 in log2 space. Complete dataset from which
Figure 3 was created.

Lower half of Figure. Hierarchical cluster analysis of genes and experiments with
different DHT treatment regimens. Shown are the 2862 transcripts that distinguish
between normal genital skin fibroblasts and gonadal fibroblasts from 46, XY female
AIS patients, and between proliferating and confluent fibroblasts. The color code
in the dendrogram depicts the origin of the fibroblast cultures. The gray and white
bars on top of the cluster indicate the proliferation state of the samples. On the
right, the regions of the cluster diagram are indicated which differentiate between
normal and AIS-derived fibroblasts, and proliferating and confluent cells, respectively.
No differences in transcript levels could be discerned between DHT treated and control
cells in either normal foreskin fibroblasts or fibroblasts from AIS affected 46, XY
females. The scale ranges from -8 to +8 in log2 space. Complete dataset from which
Figure 3. was created.