An investigation entitled “Induced chlorophyll mutations in black gram” was conducted during
kharif (rainy season) 2004 at the field experimentation centre of the Department of Genetics and Plant
Breeding, Allahabad Agricultural Institute - Deemed University, Allahabad. Seeds of IPU-982 of Black gram
were exposed to gamma rays and then treated with sodium azide to obtain the spectrum and frequency of
chlorophyll mutations in M2 generation Combination treatment of gamma rays with sodium azide enhanced the
frequency of chlorophyll mutations in M2 generation. The treatments of sodium azide were found to be more
efficient than gamma rays in inducing chlorophyll mutations when applied singly. A progressive increase in
mutation frequency of chlorophyll mutations w as observed with increasing doses. Six different types of
chlorophyll mutants, namely, albino, xantha, dark xantha, chlorina, viridis and striata were induced. Out of
these mutants, xantha and dark xantha were most frequent while striata was least frequent. The highest
frequency of chlorophyll mutations (8.87) was reported in the combination of 60kR+0.03%SA. There was a
dose dependent increase in the spectrum and frequency of chlorophyll mutations whether mutagens were
employed singly or in combination.