Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

WB

1/1000. Predicted molecular weight: 52 kDa.

IP

Use at an assay dependent dilution. Use at a concentration of 5 - 10 µg. The protein does not retain the kinase activity after immunoprecipitation.

ELISA

1/1000.

ICC/IF

Use at an assay dependent dilution. PubMed: 19047045

Target

FunctionParticipates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.

Tissue specificityExpressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.

Post-translationalmodificationsPhosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216.

Cellular localizationCytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.

Thank you for your enquiry. The source of ab2602 replied to us; "In our hands we see only two bands. The weaker one, we believe is the GSK3-alpha. We have no evidence of its reactivity with GSK2-alpha."
I hope this information helps, please do not ...

My sincere apologies for the delay in responding to you, I have just this minute received this information from the source of ab2602:
"This monoclonal antibody will work well in immunoprecipitation, however, its affect on the GSK3 kinase activity has ...