mouse bone marrow MSC culture

i am culturing the bone marrow cells of balb/c mice of 6-8 weeks. after 3 days of plating mesenchymal cells start appear(fibroblast like). But the MSCs do not multiply and after 14-15 days of culture, those cells die. I use DMEM+L-glutamine media. Please suggest me about how to get a confluent culture.

mouse BM MSC sometimes takes long time for them to start growing after isolation, so it sounds normal for me the isolated cells are not growing right after the isolation.
But in your case, cell is dying, so first of all, I will make sure the initial seeding density, then will check the medium (especially quality of FBS)

Dear all, I could finally manage to grow the MSCs from bone marrow primary culture .

But, i face a new problem.
m unable to detach all cells from a T25 flask by Trypsin EDTA. I keep the trypsin at 37degree b4 using. I wash the cells twice with TPVG and add 2ml more TPVG and keep for 2-3 min at 37 degree but still all cells don't detach.
Due to repeated TPVG exposure cells are also getting stressed.