ENGLISH ABSTRACT: This study describes:
I. The cloning of the CVP 19 gene and construction of the intracellular expression
vector pPIC3.5K-CYP19.
II. The transformation of the yeast, Pichia pastoris with the constructed vector.
III. The expression ofP450arom in Pichia pastoris.
IV. The determination of enzyme activity and isolation of the protein from the Pichia
pastoris cells.
V. The expression of P450c 17 in Pichia pastoris.
VI. The determination of kinetic constants for the conversion of progesterone to
170H-progesterone and 160H-progesterone by P450c17.