ZEGSUS wrote:http://aem.asm.org/cgi/reprint/60/11/4192.pdfWell I guess that is possible to insert "naked" DNA into bacteria.

yeah, but I guess it depends on the species. Agrobacterium is pathogenic bacteria and some of its relatives (I don't know whether Agrobacterium by itself) can posses linear plasmids. Such bacteria will of course accept "naked" DNA (but still the problem with replication), but it's questionable how will E.coli do.

ZEGSUS wrote:http://aem.asm.org/cgi/reprint/60/11/4192.pdfWell I guess that is possible to insert "naked" DNA into bacteria.

yeah, but I guess it depends on the species. Agrobacterium is pathogenic bacteria and some of its relatives (I don't know whether Agrobacterium by itself) can posses linear plasmids. Such bacteria will of course accept "naked" DNA (but still the problem with replication), but it's questionable how will E.coli do.

E. coli will not readily take up naked DNA. Other species can, but not E. coli.If you are looking for a specific gene from Acetobacter bulgaricus your best bet is indeed to try a BAC. There are many kits commercially available (Google "BAC cloning kit" and your country of residence to find some supplier, or ask your friendly local sales rep) to do just that. And no, not all bacteria will accept DNA by electroporation. But in your case that should not be a problem.

Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)