International Journal of Molecular and Cellular Medicineمجله بین المللی سلولی و مولکولیInt J Mol Cell MedMedical Scienceshttp://ijmcmed.org1admin2251-96372251-964510.22088/IJMCM.BUMSenjalali139441gregorian20157143online1fulltextenExpression of Melanocortin 4 Receptor mRNA in Male Rat Hypothalamus During Chronic StressNeurosciencesNeurosciencesOriginal ArticleOriginal Article<p>The effects of chronic stress and glucocorticoids receptor antagonist (RU486) on expression of melanocortin 4 receptor (MC4R) mRNA in arcuate nucleus (ARC) of male rats were evaluated. In this study, adult male Sprague Dawley rats were placed into four groups (n=6/group) stress, RU486, stress/RU486, and control groups. In stress group, the rats were restrained, 1 h/day, for 12 days. In RU486 group, the rats were injected RU486 for 12 days. In stress/RU486 group, the rats were injected RU486 1 h before the stress process for 12 days. Relative expression of MC4R mRNA was determined using real-time PCR. Relative expression of MC4R mRNA in the stress group was higher than that of the control rats (P<0.05). Relative expressions of MC4R mRNA were not different between the stress, RU486 and stress/RU486 groups (P>0.05). Chronic restraint stress causes increase in mRNA expression of MC4R in ARC and blockade of glucocorticoid receptors has no effect on this up-regulation.</p>
Chronic stress, melanocortin 4 receptor (MC4R), hypothalamus, rats182187http://ijmcmed.org/browse.php?a_code=A-10-212-2&slc_lang=en&sid=1MaryamKarami Kheirabadmarya.karami@gmail.com1003194753284600550210031947532846005502NoDepartment of Basic Sciences, Azad University, Gachsaran Branch, Gachsaran, Iran.BahiaNamavar Jahrominamavarb@sums.ac.ir1003194753284600550310031947532846005503NoInfertility Research Center, Department of OB-GYN, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.AminTamadonamintamaddon@yahoo.com1003194753284600550410031947532846005504Yes
Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.AminRamezaniamin.ramezani@gmail.com1003194753284600550510031947532846005505NoDepartment of Medical Biotechnology, School of Advanced Medical Sciences and Technology, Shiraz University of Medical Sciences, Shiraz, Iran.SomayehAhmadlooparisahmadloo2014@gmail.com1003194753284600550610031947532846005506NoInfertility Research Center, Department of OB-GYN, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.FatemehSabet Sarvestanidfssarvestani@yahoo.com1003194753284600550710031947532846005507NoInfertility Research Center, Department of OB-GYN, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.OmidKoohi-Hosseinabadikoohiomid@yahoo.com1003194753284600550810031947532846005508NoLaboratory Animal Center, Shiraz University of Medical Sciences, Shiraz, Iran.