Datasheet Extract

Immunogen

Immunogen:

Pooled whole dog serum and partly purified serum fractions. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Application

In precipitating techniques as immunoelectrophoresis and radial immunodiffusion (Ouchterlony) to identify the serum protein pattern, or the presence or absence of an individual component. To evaluate the purity of an isolated serum protein including immunoglobulins. Since immunoprecipitation depends on a correct antigen/antibody concentration ratio (zone of equivalence) in the gel medium, the protein analysis by immunoelectrophoresis of serum or any other biological fluid or protein fraction should include different proportions of the reactants. It is not possible to obtain an optimal protein pattern in a single analysis. The electroendosmosis effect of different types of agar on proteins with a different net charge can be used to optimize the resolution power of the test system. Agar contains sufficient positively charged ions to optimize the resolution of the proteins in the beta-gamma regions, while the alpha regions will become more dense. Highly purified agar with low electroendosmosis favours the resolution of the proteins in the alpha regions, while the major components in the beta-gamma region can still be identified.Directions for use:In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum.In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.

Storage

Prior to reconstitution store at 2-8°C.Following reconstitution store undiluted at 2-8°C for one weekor (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.Shelf life: one year from despatch.

In immunoelectrophoresis against pooled serum and enriched serum proteins fractions precipitation can be observed of not less than 14 individual proteins components.Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to animal proteins since homologous proteins of different species frequently share antigenic determinants. This antiserum has not been adsorbed for such cross-reactivity. Consequently it is not species-specific.