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My research involves making lentiviral vectors pseudotyped with vesicular stomatitis virus envelop glycoprotein (VSV-G). In this protocol, I transfect HEK-293T cells with 3 plasmids; one for ... read more

PolyJet works very well, especially in HEK293 series cells, such as HEK293, HEK293T, HEK293FT. Compared to other transfection reagents, it is much less expensive but shows same efficiency of ... read more

The M-bcr FusionQuant® Kit is used for the quantitative detection of M-bcr fusion transcripts on real-time thermal cyclers. Ipsogen has designed a very sensitive, reliable and easy to use kit. This ... read more

In our experiment, I used Qiagen’s QIAEXII Gel Extraction Kit (Cat. No. 20021) to prepare the DNA prior to transfection so that it was clear of bacterial endotoxins. I used the “desalting protocol” ... read more

Transfection and expression of altered genes into cell lines are indispensable parts of genetic studies. There are many different methods for introducing genetic material into eukaryotic cells, ... read more

A common method used in almost every lab these days is transient transfection of cell lines followed by some form of output (Western blot, reporter gene assays, etc.). In a majority of cases, one ... read more

Efficient and effective transfection reagents are essential for protein expression in eukaryotic cells. Recent years have seen a rapid expansion in the number and type of transfection reagents ... read more

Invitrogen have added a new product, Lipofectamine™ LTX, to their extensive range of transfection products. Following on from other products in the Lipofectamine range, Lipofectamine™ LTX is a lipid ... read more

Lipofectamine™ 2000 Reagent is a lipid-derived reagent designed to deliver exogenic nucleic acids (DNA and/or RNA) into eukaryotic cells, either adherent or in suspension. Thee two most important ... read more

Transfection reagents are big business. The ability to express exogenous genes within eukaryotic cells is very popular and companies are falling over themselves marketing these products, claiming to ... read more

Transfection reagents and protocols have become very widespread and utilized in many aspects of DNA research. There are many applications that require the introduction of vectors and expression ... read more

The objective of lipid cationic delivery is to obtain the highest efficiency possible, while minimizing the level of cell death. Most of the reagents used require plating of the cells at a high ... read more