Graduate School of Health and Nutrition Sciences, Nakamura Gakuen University

抄録

A micellar electrokinetic capillary chromatographic (MEKC) method was developed for the two major polymethoxylated flavones, nobiletin and tangeretin, using a fused-silica open-tubular capillary in a 70% methanol extract from citrus albedo tissue. A MEKC separation buffer (pH 7.0), consisting of 32mM sodium dodecyl sulphate (SDS), 20mM sodium dihydrogen phosopate, 12mM disodium tetraborate and 20% methanol, was selected in order to optimize separation conditions. The order of elution was nobiletin and tangeretin. Of the 46 species and cultivars in the <I>Citrus </I>genus, 17 were assayed for nobiletin and/or tangeretin in this study. The Acrumen and Pseudofortunella sections, classified by Tanaka, were found to contain the highest flavone concentrations.