The result showed that the percentage of parthenogenesis and degeneration of nuded oocytes (absent of cumulus cell) was significantly higher (P<0.05) than oocyte with 5 layers of cumulus cells. In contrast the highest maturation rate was found in 5 layers COCs while the lowest maturation rate was found in nude oocytes.

Conclusion

The present study showed that the cumulus cell had an effect on meiosis of the domestic cat oocyte. Cumulus cells more than 2-layers were able to increased normal meiosis and inhibited parthenogenesis of the domestic cat oocyte.

Treatment

>5 layers *

2-5 layer

1 layer

None

Parthenogenesis

2/101 (2)a

1/135 (7) ab

6/137 (4) ab

11/125 (9) b

Degeneration

17/101 (17)a

24/135 (18)a

31/137 (23)a

44/125 (35)b

Oocyte maturation

62/101 (61)a

74/135 (55)a

58/137 (42)b

25/125 (20)c

* layer of cumulus cell

a-c Values with different superscripts show significant difference among groups of COCs (rows) (P<0.05)