Sequencing through poly A region

Dear Friends,
Would someone like to share with me his/her experience of sequencing
through a region of ~22 As in Arabidopsis. I have tried different
chemistries from ABI and Amersham but both fail to produce any good
sequence. It produces very nice and clean sequence till the end of the A
stretch but fails right after that. I have not tried using anything
different in the reaction yet. Recently I heard about Dynamic ET poly T
primer kit of Amersham but haven't tried it either. Any advice of any
sort
will be appreciated.
Cheers!
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Muhammad A. Lodhi Phone: (516)367-8854(O)
Lita Annenberg Hazen Genome Center (516)367-6923(H)
McClintock Lab
Cold Spring Harbor Laboratory Fax: (516) 367-8874
One Bungtown Road,
Cold Spring Harbor, NY 11724 E-mail: lodhi at cshl.org
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