When equivocal results are obtained, another specimen should be drawn 10-14 days later and tested in parallel with the initial specimen. If the second specimen is also equivocal, the patient is negative for primary or recent infection and equivocal for antibody status. If the second sample is positive, the patient can be considered to have a primary infection. The conversion of an individual patient’s serum from negative to positive for antibodies to the infectious agent in question is defined as seroconversion and indicates active or recent infection.