Institute of Synthetic Biology and Cluster of Excellence on Plant Sciences (CEPLAS), University of Düsseldorf, Düsseldorf, Germany.Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Freiburg, Germany.

Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Freiburg, Germany.Faculty of Biology, University of Freiburg, Freiburg, Germany.BIOSS Centre for Biological Signalling Studies, University of Freiburg, Freiburg, Germany.

Abstract

Strigolactones are key regulators of plant development and interaction with symbiotic fungi; however, quantitative tools for strigolactone signaling analysis are lacking. We introduce a genetically encoded hormone biosensor used to analyze strigolactone-mediated processes, including the study of the components involved in the hormone perception/signaling complex and the structural specificity and sensitivity of natural and synthetic strigolactones in Arabidopsis, providing quantitative insights into the stereoselectivity of strigolactone perception. Given the high specificity, sensitivity, dynamic range of activity, modular construction, ease of implementation, and wide applicability, the biosensor StrigoQuant will be useful in unraveling multiple levels of strigolactone metabolic and signaling networks.

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