Human Lung Microvascular Endothelial Cells (HLMVEC) Culture Protocol

I. Storage

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liquid nitrogen in the cryovials to burst and cause injury.

Make sure all serological pipettes, pipette tips and reagent solutions are sterile.

Follow the standard sterilization technique and safety rules:
a. Do not pipette by mouth.
b. Always wear gloves and safety glasses when working with human cells even though all the strains have been
tested negative for HIV, Hepatitis B and Hepatitis C.
c. Handle all cell culture work in a sterile hood.