...uid Kit provides a sensitive and rapid method of separating and detecting oligoclonal immunoglobulin bands in unconcentrated cerebrospinal fluid using isoelectric focusing. The presence or absence of these bands is important as supporting evidence for the diagnosis of central nervous system (CNS) disorders. This...

Description:... Agarose IEF is suitable for isoelectric focusing of large molecules and is the gel of choice for many IEF applications and immunochemical detection methods....Company:GE Healthcare, formerly Amersham Biosciences

... were processed with the ReadyPrep 2-D cleanup kit to remove salts and
detergents and to create a low-conductivity sample suitable for isoelectric
focusing (IEF). This cleanup kit was also employed, if required, to concentrate
proteins from dilute samples. Prior to IEF all s...

... of proteins.
Methods
Preparation of isoelectric Focusing Strips
Cells from a human cell line were lysed with 7 ...nsistent with the exception of some minor differences due to the
isoelectric focusing step. The highly expressed proteins did not suffer
from...

...y bound membrane
proteins (E3). Samples were either subjected to isoelectric focusing immediately
following extraction or stored at -80C unti...hydrated overnight in a humidity
chamber. Once fully rehydrated, isoelectric focusing of proteins was carried
out with the PROTEAN IEF cell u...

...n (TOBBLP4, gift from
Dr. J. Denecke). 3
Preparative isoelectric Focusing
Fractions 35, 36, and 37 from the Model 491 Prep Cell...fractions 3537 from the Model 491
Prep Cell run using preparative isoelectric focusing produced
what appears to be a highly pure form of BiP in...

Description:...y in solution. Precipitated proteins can easily be re-suspended in the buffer of choice for a wide range of proteomics downstream applications such as isoelectric focusing (IEF), 2DGE, 1DGE or for tryptic digestion prior to mass spectrometry and peptide separation schemes. • The ProteoExtract™ Protein...Company:Calbiochem

Description:... The Rotofor purification system with PowerPac 3000 power supply, 220-240 V, is used for protein separation and purification by liquid-phase isoelectric focusing (IEF). The system includes both the mini (18 ml) and standard (60 ml) focusing chambers, chassis, harvesting apparatus, cooling finger, elect...Company:Bio-Rad

Description:... to provide a biotin-binding protein with exceptionally low nonspecific binding properties. NeutrAvidin does not contain carbohydrate and has an ideal isoelectric point (6.3) for the best utility in a variety of applications.
For assay development, Pierce has coated NeutrAvidin onto microplates and pre-blocked ...Company:Pierce Biotechnology, Inc.

Description:...c 3000 power supply is the most versatile power supply available, ideal for isoelectric focusing, DNA sequencing, isotachophoresis, SDS-PAGE, 2-D electrophoresis, ...he PowerPac 3000 power supply offers enough power to run the most demanding isoelectric focusing experiments, or up to four DNA sequencing cells at the same time....Company:Bio-Rad

...
Gel electrophoresis is a group of techniques used by scientists to separate molecules based on physical characteristics such as size, shape, or isoelectric point . Gel Electrophoresis is usually performed for analytical purposes, but may be used as a preparative technique to partially purify molecules to...

...
Gel electrophoresis is a group of techniques used by scientists to separate molecules based on physical characteristics such as size, shape, or isoelectric point . Gel Electrophoresis is usually performed for analytical purposes, but may be used as a preparative technique to partially purify molecules to...

...teins can be separated based on size using an SDS-PAGE gel. Proteins can also be separated based on their electric charge , using what is known as an isoelectric gel...
Western blotting and immunochemistry
Main article: Western blot
Antibodies to most proteins can be created by injecting small amou...

...ly been practiced through the separation of proteins by two dimensional gel electrophoresis . In the first dimension, the proteins are separated by isoelectric focusing , which resolves proteins on the basis of charge. In the second dimension, proteins are separated by molecular weight using SDS-PAGE . T...

...
Key technologies used in proteomics
One and two-dimensional gel electrophoresis are used to identify the relative mass of a protein and its isoelectric point .
X-ray crystallography and nuclear magnetic resonance are used to characterize the three-dimensional structure of peptides and protein...

...ad, the polymerized tubulin forms two-dimensional crystalline sheets that ... s of a- and ß- tubulin . These subunits are slightly acidic with an isoelectric point ... Tubulin was long thought to be specific to eukaryote. Eukaryote ... Drosophila has four ß- tubulin genes: each encodes a distinct is...

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