This 96-well kit provides a rapid method for the high throughput isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, or yeast.

The kit employs two plates: 1) for DNA purification and 2) for RNA purification utilizing silicon carbide resin (superior for the binding of all RNA sizes including miRNA). Please see the protocol schematic below.

The proteins are also purified on the second plate after RNA elution. The proteins are eluted in buffer and are ready for downstream applications without any further clean up required. The proteins can be quantified directly, used in western blots, ELISA or mass spectrometry.

Norgen's RNA/DNA/Protein Purification 96-Well Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as stem cells, CTC, biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA, DNA and proteins are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications.

Kit Specifications

Binding Capacity Per Well

50 μg for RNA
20 μg for DNA
150 μg for protein

Size of RNA Purified

All sizes, including
small RNA (< 200 nt)

Size of DNA Purified

≥ 30 kb

Time to Complete 10 Purifications

45 minutes

Maximum Amount of Starting Material:

Animal Cells

Animal Tissues

Blood

Bacteria

Yeast

Fungi

Plant Tissues

1 x 106 cells

10 mg

100 µL

1 x 109 cells

1 x 108 cells

40 mg

40 mg

Average Yields*
Liver (5 mg)

RNA: 12.5 μg
DNA: 2 μg
Protein: 55 μg

* average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.