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Technical Abstract: Acoustic methods were employed to assess the effect of hermetic environments and controlled atmospheres on the behavior and mortality of Sitophilus oryzae and Callosobruchus maculatus. Six treatments of 25, 50 and 100 insects were sealed hermetically in 500- and 1000- ml glass jars for 28 days. Data on S. oryzae showed that their acoustic activity decreased to <0.02 bursts/s within 3–14 d depending on initial infestation levels, as oxygen fell below 5% and mortality was observed at 2% oxygen level. Data on C. maculatus showed that their acoustic activity decreased to <0.02 bursts/s within 3-11 d depending on initial infestation levels when oxygen levels declined to below 5% and mortality was observed at 4%. Linear regression showed statistically significant relationships between the acoustic activity and the residual oxygen level for both species. An equation was made to estimate the time needed for oxygen to decline to levels that limit insect damage.