Application Information: Bid antibody

IP: Use at a concentration of 20 μg/ml.WB:Use at a concentration of 0.5-5 µg/ml. Optimal dilutions/concentrations should be determined by the end user.

Form Supplied

Liquid

Concentration

0.5
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Immunogen affinity purified

Specifications: Bid antibody

Full Name

BH3 interacting domain death agonist

Product Description

Rabbit polyclonal to Bid

Background

Bid, a BH3 domain-containing pro-apoptotic Bcl-2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent pro-apoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In the animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.

Synonyms

Bid, 637, MGC42355, 601997, P55957, MGC15319

Cellular Localization

Cytoplasmic

Specificity

This antibody detects both full length (22 kDa) and cleaved fragment (15 kDa) of mouse Bid.

Host

Rabbit

Clonality

Polyclonal

Target

Bid

Isotype

IgG

Immunogen

Synthetic peptide surrounding amino acid 45 of Mouse Bid.

Antigen Species

Mouse

Species Reactivity

Mouse

Storage Conditions: Bid antibody

Storage Buffer

PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% thimerosal

Storage Instruction

Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Notes

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.