Purification of Green Fluorescent Protein Kits

Teach your students how to purify a green fluorescent jellyfish protein produced in E. coli!

Bringing genetic engineering and protein purification into your classroom has never been simpler. Students get the opportunity to create GFP-expressing bacteria, which glow in the presence of UV light, and then isolate and purify the GFP from the E. coli cells. This lab activity is available in modules, allowing you to custom tailor the learning experience to your classroom objectives and timelines.

Overview

Intermediate/Advanced - Modules 1 and 2 require some background knowledge. Module 3 is for more experienced classes and requires some technical skill.

These kit modules are great for teaching:

The molecular basis of heredity

The relationship between genes and proteins

How E. coli is used to engineer proteins useful in medicine and manufacturing

Three techniques - transformation, protein purification, and protein gel electrophoresis - commonly used in biological research and its areas of application

The lab is set up in 3 modules that can be purchased separately and used in conjunction with one another.

Module 1, the Green Gene Colony Transformation Kit (item #211080, #211080P, #211082, or #211082P), is necessary for creating the GFP-expressing bacteria used in the protein purification exercise in Module 2.

Module 2 (item #211072 or #211072P) contains all of the reagents and instructions necessary for purifying GFP from E. coli.

Module 3 (item #211073 or #211073P) contains the acrylamide gels and other electrophoresis reagents necessary for electrophoretic analysis of the purified GFP. The 3rd module is entirely optional but greatly enhances the teaching value of the lab.

Note:Order the kit modules with the perishable materials included or with a prepaid coupon to request perishables later at your convenience. Contact us or return each coupon to request delivery of perishable materials. Refrigerate Module 3’s polyacrylamide gels and protein marker until use. Sold only to schools and businesses.