FCS EXPRESS: Flow Cytometry Application Examples

Flow Cytometry Applications Examples

FCS Express is one of the premier tools for analysis and reporting of your flow cytometry data. The application examples below illustrate how FCS Express can be used to create stunning reports while easily performing complex analysis. Jump to Image Cytometry Applications.

Cell Phenotyping

Phenotyping of cells is a common application in Flow Cytometry. FCS Express provides a way to conveniently report all cell type subsets, calculate custom statistics, and display data in bar charts for visualization.

Stain Index

Stain index is easily calculated in FCS Express by using the stain index function from the Custom Tokens dialog. Simply drag and drop statistics from your plots or tables to enter the positive and negative median values for the formula as well as the negative population standard deviation. Once complete, the custom token for Stain Index will update in real time as gates or data change in your analysis. View our Stain Index information page for more details on how and why to use the Stain Index metric.

Cell Cycle Statistics and Reporting

One of the earliest applications of flow cytometer was the measurement of DNA content in cells. The analysis is based on the ability of certain dyes to stain cellular DNA in a stoichiometric manner and DNA content histograms require mathematical analysis in order to extract the underlying G1,S, and G2 phase distributions.

CBA Analysis and Reporting

FCS Express allows quick analysis and reporting of cytometric bead assays through the use of live updating regression plots and statistics.

High Content Analysis and Reporting

The High Content Add-on for FCS Express allows you to plot any plate based analysis in heat maps, derive plate and well based statistics, and use well gates to isolate wells, groups of well, and populations of interest.

High Content Analysis With Dose Response

FCS Express compliments any high content system by providing easy to use software with robust reporting, statistical, and batch processing features. FCS Express works at the plate, well, and single cell level and can generate dose response curves that update dynamically as gates are adjusted.