ExiProgen™ EC-Disulfide Protein Synthesis Kit

ExiProgen™ EC-Disulfidee Protein Synthesis Kit enables it to automatically synthesize target protein with disulfide bonds. It can be much more effectively used than cell based protein expression system because it can synthesizes protein with up to nine disulfide bonds.

This kit is used for synthesizing a protein that contains disulfide bonds using ExiProgen™. Used in conjunction with ExiProgen™, it can synthesize a protein that contains up to nine disulfide bonds in a fully automated manner and more efficiently than using cell-based synthesis. After adding template DNA and associated reagents into ExiProgen™ and using protocol No. 905, the target protein can be synthesized within a day. All processes, including protein synthesis and dialysis, are performed in an automated manner and the final protein is harvested in any storage buffer of choice.

Features and Benefits

Protein synthesis with disulfide bond
This kit is used to synthesize a protein that has two or more disulfide bonds, providing its intact function.

Parallel processing system
1-8 different proteins can be synthesized in a single run.

Procedure and principle

This kit uses oxidative environment during protein synthesis which is different from the conventional reducing environment. The energy source will be continuously provided to the reaction, and the target protein is purified using Ni-NTA magnetic beads.