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Abstract:

The invention relates to a cosmetic composition containing lingonberry
extract and its use on skin cosmetology thereof. The present invention
has provided a lingonberry extract wherein containing polyphenols
components including anthocyanidin, procyanidin, resveratrol, catechin
and so on. The present invention also relates to the application of
lingonberry extract on skin cosmetology, including without limitation
whitening, diminishing visible pigmentation, anti-wrinkle, moisturizing,
smoothing and improving skin sensitivity. The invention also has revealed
drugs, cosmetic composition and food prepared by using lingonberry
extract. Comparing with the prior art cosmetic compositions, the said
lingonberry extract of the present invention has higher effects and safe.

Claims:

1. A lingonberry extract, including at least one of anthocyanidin,
procyanidin and resveratrol.

2. A lingonberry extract according to claim 1, wherein the percentage of
the said anthocyanidin is about 5.about.35 wt %.

3. A lingonberry extract according to claim 1, wherein the percentage of
the said procyanidin is about 20.about.80 wt %.

4. A lingonberry extract according to claim 1, wherein the percentage of
the said resveratrol is about 0.01.about.50 wt %.

5. A lingonberry extract according to claim 1, wherein the said extract
comprising anthocyanidin about 5.about.35 wt %, procyanidin about
20.about.80 wt %.

7. A use of the lingonberry extract according to claim 1 on drug
preparation, wherein the said drug can be used in preservation or
treatment of monophenolase activity abnormality syndrome.

8. A drug composition containing the said lingonberry extract according to
claim 1, wherein the said drug composition can be used to inhibit the
activity of monophenolase.

9. A drug composition according to claim 8, wherein the percentage of the
said lingonberry extract is about 0.0001 wt %˜100 wt % of the total
weight.

10. An application of the said lingonberry extract according to claim 1 on
preparation of cosmetic or skin care composition.

11. An application according to claim 10, wherein the said cosmetic or
skin care composition can be applicated on melanogenesis inhibition,
clearance on skin pigmentation, colored spot and acne.

12. An application according to claim 10, wherein the said cosmetic or
skin care composition can be applicated on aging skin renovation, skin
moisture content elevation, skin flexibility enhancement, anti-wrinkle
and anti-aging.

13. An application according to claim 10, wherein the said cosmetic or
skin care composition can be applicated on skin smoothing and skin
sensitivity regulation.

14. A cosmetic or skin care composition, wherein containing the said
lingonberry extract according to claim 1.

15. A cosmetic or skin care composition according to claim 14, wherein
also has other elements selected one or several from the group consisting
of other kinds of fruit extract, collagen protein, hyaluronic acid, and
other carrying agent and vehiculum that can be used in cosmetology,
wherein the said other kinds of fruit extract can be selected from the
group consisting bilberry extract, grape extract, pomegranate extract,
rosa roxburghii extract, apple extract and kiwi fruit extract.

16. A food having cosmetic function, wherein having the said lingonberry
extract according to claim 1.

17. A food having cosmetic function according to claim 16, wherein also
contains other components selected one or several from the group
consisting of other kinds of fruit extract, collagen protein, hyaluronic
acid, and other carrying agent and vehiculum that can be used in food,
wherein the said other kinds of fruit extract can be selected from the
group consisting bilberry extract, grape extract, pomegranate extract,
rosa roxburghii extract, apple extract and kiwi fruit extract.

18. A food having cosmetic function according to claim 17, including
without limitation candy, cake, soup powder, milk product and beverages.

19. A method for skin care, including application the said cosmetic or
skin care composition according to claim 15 on the skin area needed
treatment, or taking the said food having cosmetic function orally
according to claim 18.

[0002]The invention relates to a lingonberry extract and its uses in
cosmetology. The present invention provides a lingonberry extract which
contains anthocyanidin about 5˜35wt %, procyanidin about
20˜80 wt %, resveratrol about 0.01˜50 wt %. The present
invention also relates to the application of the lingonberry extract in
cosmetology, including but not limited to whitening, diminishing visible
pigmentation, anti-wrinkle, moisturizing, smoothing and improving skin
sensitivity. The present invention also relates to drugs, cosmetic
compositions and foods containing the lingonberry extract. Comparing with
the prior art cosmetic compositions, the lingonberry extract of the
present invention has higher effects and safety.

DESCRIPTION OF THE RELATED ART

[0003]Lingonberry (Vaccinium vitis-idaea L.) also named Hongdou,
Xiaopingguo, Yageda, Xiongguoye in China, which belongs to Ericaceae
Vaccinium, is perennial hardwood or evergreen shrub or dwarf shrub.
Lingonberry, originally growing in mountain areas of Northeast of China,
Former Soviet Union, North Korea, North America and North Europe, which
mixed-grows with blueberry, is a kind of ever-green dwarf shrub. The
height of the tree is about 15-30 cm with evergreen herbaceous leaves. It
has strong ability of cold and drought Resistance. The fruit of
lingonberry with bright red color and sour-puckery flavor is good to
pulmonary and has high value in use.

[0004]Japanese patent application JP3826698B2 disclosed a cosmetic
composition which was made from extract of lingonberry leaves and fruits
compounding with other excipients. The lingonberry extract disclosed in
that patent was gained by the following steps: using lingonberry's leaves
and fruits as raw material to extract with alcohol-water solution,
condensing the extract solution, handling the extract solution with
resin. But deficients of the extract made by the method of the patent
are: (i) the extract's ingredients being complicated and uses limited for
the mixed raw material composed of lingonberry's leaves and fruits; (ii)
only for external use; (iii) wasting materials as the method cannot
extract the effective ingredients sufficiently in lingonberry.

[0005]PCT application WO2006134583 disclosed a cosmetic composition having
lingonberry extract. But the invention still used a extract of
lingonberry's leaves and fruits (occupying about 50% individually), which
still had the deficiencies of Japanese patent JP3826698B2 and also only
was for external use.

[0006]As a result, there is a demand on safe lingonberry extract with good
efficacy. And at the same time, lingonberry extract applications are
needed to be exploited further. Thereby the effective ingredients of
lingonberry extract are needed to be more identified, whose safety and
efficacy should be proved and usage fields should be expanded.

SUMMARY OF THE INVENTION

[0007]The aim of the present invention is to provide lingonberry extract
to overcome one of problems of the prior art mentioned above.

[0008]The lingonberry extract extracting method provided in the present
invention can be the same as conventional method, including but not
limiting to the art disclosed in Chinese patent application CN101077864A,
which is incorporated here as reference.

[0009]A preferable method to produce lingonberry extract includes
following steps:

[0010](1) Extracting, using the fruit of lingonberry as raw material, with
aqueous acid at normal temperature, or with aqueous alcohol at normal or
lower temperature, and then filtrating to obtain lingonberry extract
solution;

[0012](3) Drying the said purified exact obtained in step (2) to get
lingonberry exact part rich in anthocyandin;

[0013](4) Refining pomace obtained in step (1) after extracting with
aqueous acid or aqueous alcohol and filtrating by silica gel column
chromatography separation to get lingonberry compound rich in
resveratrol;

[0014](5) Mixing lingonberry exact part rich in anthocyandin and the said
lingonberry exact part rich in resveratrol at appreciable proportion to
obtain three kinds of lingonberry extract, which are lingonberry extract
I, II and III respectively. Lingonberry extract I contains anthocyandin
about 15%, procyanidin about 60% and resveratrol about 0.01%; lingonberry
extract II contains anthocyandin about 5%, procyanidin about 10% and
about resveratrol 20%; lingonberry extract III contains anthocyandin
about 10%, procyanidin about 35% and resveratrol about 10%.

[0015]The main compounds in the said lingonberry extracts are detected by
the following methods:

[0022](3) A tartaric acid or Folin-C method for detecting resveratrol in
lingonberry.

[0023]The lingonberry extract of the present invention is preferably
selected from the lingonberry fruits extract.

[0024]The present invention also relates to a cosmetic use of at least one
of the said lingonberry extract in present invention.

[0025]The said lingonberry extract of the present invention can also be
exploited as a series of cosmetic products. Skin is a kind of connective
tissue containing collagen protein and elastin which are both important
to the whole structure of skin. Moderate cross linkage of collagen is
good to maintain integrity of skin. Whereas oxidation from free radical
in vivo can make the collagen over cross link, and as a result wrinkle
and vesicle appear on the surface of the skin. The lingonberry extract of
the present invention can improve the moderate cross linkage of collagen
protein and clear free radical efficiently to keep skin from aging,
remaining white and bright. In addition, elastin can make skin resilient.
If there is not enough elastin, the skin will be relaxation to appear
old. Elastin can be degradated by free radicals and elastase. The
lingonberry extract of the present invention can clear the free radicals,
block emergence and activity of elastase to improve the condition of
skin. Researching on skin hydroxyproline content and SOD activity of skin
photoaging mice by ultraviolet radiation, it is revealed that the
hydroxyproline content and SOD activity of the mice taking the said
lingonberry extract of the present invention are all higher obviously
than the model group, which illustrates that the lingonberry extract can
protect the skin collagen protein through its excellent anti-oxidation to
appear anti-wrinkle and anti-aging function on cosmetology.

[0026]Otherwise, other use of lingonberry extract of the present invention
on cosmetology are also disclosed, including but not limiting to
whitening, diminishing visible pigmentation, anti-wrinkle, moisturizing,
smoothing and improving skin sensitivity.

[0027]The lingonberry extract of the present invention used as an ideal
melanogenesis suppressant has characteristics as follows:

[0039]The present invention also provided a cosmetic or skin care
composition used as whitening containing the lingonberry extract as
active component. The cosmetic or skin care composition of the present
invention may also have common adjuvants adding in cosmetic or skin care
products, such as hydrophilic or oleophylic gelling agent, hydrophilic or
oleophylic activator, antiseptics, anti-oxidant, solvent, perfuming
agent, sunscreen agent, pigment, fragrant absorbent and colorant. The
person skilled in the relevant field can select the dosage of adjuvants
according to need.

[0040]When the said cosmetic or skin care composition is made into cream,
lipid-phase-ratio usually is about 5˜80 wt % of the total weight,
preferably is about 5˜50 wt %, such as, but not limit to, about 10
wt %, about 20 wt %, about 30 wt %, about 40 wt % and so on. The oil ,
emulsifier and co-emulsifier used in the cream composition can be the
common material of the relevant field. The ration of emulsifier and
co-emulsifier in the cream is usually about 0.3˜30 wt % of the
total, preferably is about 0.5˜20 wt %.

[0041]The lingonberry extract of the present invention can be combinedly
used with the following kinds of oil: mineral oil, plant resource oil
(such as soya oil), animal resource oil (such as agnolin), synthesis oil
(such as perhydro-squalene). Besides, fatty alcohol, fatty acid and wax
can also be used as fatty material.

[0042]The lingonberry extract of the present invention can be combinedly
used with the following kinds of emulsifier and co-emulsifier: fatty acid
ester (such as macrogol) and fatty acid ester of glyceric such as
stearin.

[0043]According to an embodiment of the present invention, the lingonberry
extract can also be used at least one kind of activator, including but
not limiting to ellagic acid and its derivatives, hydroquinol, arbutin,
resorcin and its derivatives, vitamin C and its derivatives, pantothenic
acid sulfonate and its derivatives, cojic acid, intacellin, molecules
that can interfera α-melanotropin (α-MSH) or its receptors or
acetone (ACTH), polyhydroxy compound (such as glycerine, ethylene alcohol
or propylene glycol), vitamin, certain dissolving agent or decorticate
agent (such as ortho-oxybenzoic acid and its derivate), α-alcoholic
acid (such as lactic acid or malic acid), antiscorbic acid and its
derivatives, retinoic acid, retinal, retinol and its derivate,
anti-inflammatory agent, emollient and its mixture, chemical or physical
sunscreen agent (such as micronization zinc oxide, titanium oxide,
butyl-methoxyl-dibenzoyl-methane and octyl methoxycinnamate), deoxyribose
nucleic acid and nucleic acid.

[0047]3. A lingonberry extract containing polyphenols about 5˜100%,
wherein the polyphenols are selected from the group consisting of
anthocyanidin, procyanidin, flavone, anthoxanthin, catechinic acid,
epicatechin and its polymeride.

[0049]5. The lingonberry extract according to item 4 mentioned above
further comprising polyphenols about 5˜100%, wherein the said
polyphenols can be selected from the group consisting of anthocyandin,
procyanidin, flavone, catechin, epicatechin and its polymeride.

[0051]7. The application of the lingonberry extract according to 1˜6
mentioned above on drugs preparation, wherein the said drugs can be used
in prevention or treatment of monophenolase activity abnormality
syndrome.

[0052]8. A drug composition containing the lingonberry extract according
to items 1˜6 mentioned above, wherein the said drug composition can
be used to inhibit the activity of monophenolase.

[0053]9. The drug composition according to item 8 mentioned above, wherein
the percentage of the said lingonberry extract is about 0.0001 wt
%˜100 wt % of the total weight.

[0054]10. The application of the lingonberry extract according to items
1˜6 mentioned above on preparation of cosmetic or skin care
composition.

[0055]11. The said application according to item 10 mentioned above,
wherein the said cosmetic or skin care composition can be applied on
melanogenesis inhibition, clearance on skin pigmentation, colored spot
and acne.

[0056]12. The said application according to item 10 mentioned above,
wherein the said cosmetic or skin care composition can be applicated on
aging skin renovation, skin moisture content elevation, skin flexibility
enhancement, anti-wrinkle and anti-aging.

[0057]13. The said application according to item 10 mentioned above,
wherein the cosmetic or skin care composition can be applicated on skin
smoothing and skin sensitivity regulation.

[0058]14. A cosmetic or skin care composition, wherein containing the said
lingonberry extract according to items 1˜6 mentioned above.

[0059]15. The cosmetic or skin care composition according to item 14
mentioned above, wherein also has other elements selected one or several
from the group consisting of other kinds of fruit extract, collagen
protein, hyaluronic acid, and other carrying agent and vehiculum that can
be used in cosmetology, wherein the said other kinds of fruit extract can
be selected from the group consisting bilberry extract, grape extract,
pomegranate extract, rosa roxburghii extract, apple extract and kiwi
fruit extract.

[0060]16. A kind of food having cosmetic function containing the
lingonberry extract according to 1˜6 mentioned above.

[0061]17. The food having cosmetic function according to item 16 mentioned
above, further containing other components selected one or several from
the group consisting of other kinds of fruit extract, collagen protein,
hyaluronic acid, and other carrying agent and vehiculum that can be used
in food, wherein the said other kinds of fruit extract can be selected
from the group consisting of bilberry extract, grape extract, pomegranate
extract, rosa roxburghii extract, apple extract and kiwi fruit extract.

[0062]18. The food having cosmetic function according to item 16 or 17
mentioned above, including without limitation candy, cake, soup powder,
milk product and beverages.

[0063]19. A skin care method for applying the cosmetic or skin care
composition according to item 14 or 15 mentioned above on the skin area
needed treatment, or taking the food having cosmetic function orally
according to item 17 or 18 mentioned above.

[0064]The following embodiments will explain the present invention further
but should not be explained as the limit on the present invention.

DETAILED DESCRIPTION OF THE EMBODIMENTS OF THE INVENTION

Example 1

Preparation of Lingonberry Extract I

[0065]1000 g lingonberry fruit was immersed in 3-fold amount of water
solution and added 0.1% cellulose water solution, then mixed. The mixture
was incubated in 40° C. thermostatic waterbath for 2 hours, and
then filtered to obtain an extract solution. The extract solution was
filtered through an ultrafiltration membrane filter (molecular weight
2000) to obtain an concentrated solution. The concentrated solution after
membrane filter was purified by HP20 macroporous resin, eluted with 60%
ethanol, concentrated further and spray dried to obtain anthocyanidin
part of lingonberry.

[0066]The fruit residue after the mentioned above steps was heated and
extracted with 75% ethanol to obtain yellow cream. Then the cream was
dealt with silica gel column chromatography to collect the resveratrol
part of lingonberry.

[0067]The anthocyanidin part and resveratrol part were combined to gain
lingonberry extract I, which comprised anthocyanidin about 15%,
procyanidin about 60% and resveratrol about 0.01%.

Example 2

Preparation of Lingonberry Extract II

[0068]1000 g lingonberry fruit was extracted by 2% hydrochloric acid
ethanol water solution at 40° C. to obtain an extract solution.
The extract solution was treated by XAD16 macroporous resin columns
directly and then washed by 90% ethanol to obtain the eluant. The eluant
was dealt with vacuum concentration to remove the ethanol and spray
drying to obtain anthocyanidin part of lingonberry.

[0069]The fruit residue after the mentioned above steps was heated and
extracted with ethanol to obtain yellow cream. Then the cream was dealt
with silica gel column chromatography to collect the composition riching
in resveratrol which was then recrysted with about 95% ethanol to obtain
the resveratrol part of lingonberry.

[0070]The anthocyanidin part and resveratrol part were combined to gain
lingonberry extract II, which comprised anthocyanidin about 5%,
procyanidin about 10% and resveratrol about 20%.

Example 3

Preparation of Lingonberry Extract III

[0071]1000 g lingonberry fruit was extracted by 60% ethanol water solution
at 40° C. to obtain an extract solution. After retrieving the
ethanol, the extract solution was treated by CHPA20 ion exchange resin
and then washed with ethanol about 90% to obtain the eluant. The eluant
was dealt with vacuum concentration to remove the ethanol and spray
drying to obtain anthocyanidin part of lingonberry.

[0072]The fruit residue after the mentioned above steps was heated and
further extracted with 95% ethanol to obtain yellow cream. Then the cream
was dealt with silica gel column chromatography to collect the
composition riching in resveratrol.

[0073]The mentioned above anthocyanidin part and resveratrol part were
combined to gain lingonberry extract III, which comprise anthocyanidin
about 10%, procyanidin about 35% and resveratrol about 10%.

Example 4

Comparison Between Influences of Lingonberry Extract and Arbutin on Cell
Multiplication

[0074]B16 melanocytes (from SIBCB) were cultivated with RPMI-1640 culture
fluid containing about 10% newborn calf serum and
penicillium--streptomycin under the condition of about 37°
C., about 5% CO2. Digest the melanocytes with about 0.125% trypsin
(Difco, US) when the cell fusion reached more than about 80%.

[0075]The activity of melanocytes multiplication was determined by
measurement of titrated thymidine incorporation (MTT). The B16
melanocytes at densities of about 1×105 were inoculated in
24-well cell cultured plate. The culture fluid was changed next day and
the tested substances in different concentration were added individually:
the lingonberry extract III prepared in example 3 (comprised
anthocyanidin about 10%, OPC about 35% and resveratrol about 10%),
arbutin bought from the market, and blank as control group.

[0076]After 20 hours cultivation and adding about 0.15 ml MTT, the cells
were cultivated continuously for about 4 hours. Centrifuged at 3000 rpm
for about 10 min and deserted the supernatant, cultivated cells in every
well were added DMSO (about 1%) about 1 ml and shook for dissolving
thoroughly. Then about 150 ml cultivated cells aspiration were added into
96-well cell cultured plate. The OD value of the aspiration was measured
with MQX200 micro-plate spectrophotometer (Bio-Tek, US), at 570 nm
excitation wave length and 630 nm reference wave length.

[0077]Calculated the inhibition ratio of tested agents on melanocytes
using the following formula:

melanocytes multiplication inhibition ratio=(1-OD of tested group/OD of
control group)*100%

[0079]It can be seen from table 1 that at the same concentration the cell
survival ratios of lingonberry extract tested groups are all higher than
the arbutin groups which indicates that lingonberry extract has more
lower toxicity on B16 cells than arbutin and as a result is more safer
than the latter.

[0080]The experimental method was the same as example 4. The lingonberry
extract III was prepared by the method in example 3, pine bark extract
and grape seed extract were both bought from market, blank was used as
control group. The results was showed as the following table 2.

[0081]It can be seen from table 2 that the influences of pine bark
extract, grape seed extract and lingonberry extract at different level of
concentration on B16 cell survival ratio are almost the same, which is
augmentation according to the extracts' concentration and presents
evident dose-response relationship. It can be concluded that these three
kinds of extract are all safe to cells in vitro.

[0082]B16 melanocytes(from SIBCB) were cultivated with RPMI-1640 culture
fluid containing 10% newborn calf serum and
penicillium--streptomycin under the condition of about 37°
C., 5% CO2. The melanocytes were digested with about 0.125% trypsin
(Difco, US) to be passaged or tested when the cell fusion reached more
than 80%.

[0083]Tyrosinase activity is determined by the speed of dopa being
oxidized into dopaquinone. L-dopa was used as catalysis substrate.
Optical density (OD) of dopaquinone was determined at 475 nm by improved
Marlinez-Esparza method. (Ando H, Itoh A, Mishima Y et al., Correlation
between the number of melanosomes, tyrosinase mRNA levels, and tyrosinase
activity cultured murine melanoma cells in response to various
melanogenesis regulatory agents. J Cell Physiol, 1995, 163: 608-614.).
The higher of the OD, the stronger of the tyrosinase.

[0084]The lingonberry extract III was prepared by the method in example 3,
other materials were all bought from market.

[0085]After 3 days of effects of tested substance, the supernatant was
deserted and the remainder was washed for 3 times with pH 7.0 PBS after
centrifuging at 1000 rpm for 5 min every time. The cells were dissolved
by 1% Troton X-100 400 μl adding in every well of the cultured plate.
100 μl solution in every well was taken and then added into 96-well
cultured plate. After pre-temperature at 37° C., 100 μl 1.7%
L-dopa (sigma, US) was added into every well. The OD A value then was
determined every 10 min within 1 hour at 475 nm and 37° C., using
blank as zero adjustment.

[0086]Calculated every tested agents' inhibition rate of B16 melanocytes'
tyrosinase activity using the following formula:

inhibition rate of melanocytes' tyrosinase activity=(1-OD of tested
group/OD of control group)*100%

[0088]It can be seen from table 3 that the influences of four kinds of
substances on inhibition rate of melanocytes' tyrosinase activity are all
more and more stronger with the increasing of their concentration.
Whereas the rising amplitude of lingonberry extract test group is more
obvious. When the concentration is about 30 mg/L, the inhibition rate of
lingonberry extract on melanocytes' tyrosinase activity is much higher
than other tested groups.

[0089]B16 melanocytes(from SIBCB) were cultivated with RPMI-1640 culture
fluid containing about 10% newborn calf serum and
penicillium--streptomycin under the condition of 37° C., 5%
CO2. The melanocytes were digested with about 0.125% trypsin (Difco,
US) to be passaged or tested when the cell fusion reached more than about
80%.

[0091]B16 melanocytes (1×105) were cultivated in 6-well
cultured plate. After 24 hours incubation the solution was changed and
different kinds of tested substances in different concentration were
added respectively. The lingonberry extract III was prepared by the
method in example 3, other substances were all bought from market.

[0095]It can be seen from table 4 that pine bark extract has no apparent
inhibiting effect on melanogenesis, whereas the inhibition rate of the
other three kinds of substances were increasing gradually with the
increasing of their concentration. When the concentration reached to 30
mg/L, lingonberry extract and grape seed extract's inhibition rates of
melanogenesis reached to 30.04% and 39.26% respectively, which were all
much higher than arbutin group.

[0098]It can be seen from table 5 that inhibition rates of melanocytes'
tyrosinase activity of different kinds of extract were augmenting with
the increasing of the extracts concentration and presented evident
dose-response relationship. When the concentration <10 mg/L, the
influence was not obvious. When concentration was higher than about 20
mg/L, three kinds of extract's inhibition rates of melanocytes'
tyrosinase activity all increased obviously, whereas the influence of
lingonberry extract III was more stronger than others.

Example 9

Comparative Study Among on B16 Melanogenesis Influences of Different Kinds
of Lingonberry Extract

[0099]Experiment method was the same as example 7. The lingonberry extract
I, II and III used as tested substance were prepared by the method in
example 1, 2 and 3 respectively.

[0101]It can be seen from table 6 that inhibition rates of melanogenesis
of different kinds of extract are augmentation with the rising of the
extract's concentration, whereas the effect of lingonberry extract
quadrature is more obvious.

[0102]50 Kunming mice, weight 15˜20 g, were divided into 5 groups
randomly, that were control group, model group, tested group (high
dosage, middle dosage, low dosage). Besides control group, mice of other
groups, shaved the hair on back, were all exposed under UVA light
everyday to result in photoaging model. The animals of high dosage,
middle dosage and low dosage tested groups were given lingonberry extract
(containing anthocyanidin about 12.9%, procyanidin about 60.3% and
resveratrol about 10.1%) 160 mg/kg, 80 mg/kg and 40 mg/kg respectively,
whereas animals of control group were given distilled water of the same
volume. After animals sacrificed 8 weeks later, full-thickness skin of
the hair-shaved site on the back was taken at once and SOD activity & HYP
content of the local site were determined by biochemistry method. The
result was showed in the following table 7.

[0103]It can be seen that lingonberry extract can restrain the photoaging
procedure of skin and elevate the skin SOD activity and HYP content to
make skin vigorous and flexible again. As a result, it can be added into
health products with cosmetic effects and cosmetics.

[0105]It can be seen from the result that extract I, II and III all have
the effect of relieving facial wrinkle and diminishing visible
pigmentation, improving skin sensitivity and smoothness, whitening and
moisturizing effects. All these indexes have distinguished statistical
significance compared with the value before capsule taking (P<0.05)
and the total effective rates are all higher than 87%. Lingonberry
extract III can obviously relieve the facial wrinkle grade and colored
spot, improve skin sensitivity and smoothness. All these indexes have
distinguished statistical significance compared with the value before
capsule taking (P<0.05) and the total effective rates are all higher
than 82%. Lingonberry extract III especially has significant effect on
whitening and moisturizing. Those two indexes have distinguished
statistical significance compared with the value before capsule taking
(P<0.01) and the total effective rates reaches 100%.

Experiment 12

Candies Having Cosmetic Function Made from Lingonberry Extract

[0106]The candies were made obeying the traditional composition proportion
showed by the following table 9.

[0108]The characteristic of the present invention is, for using
lingonberry fruit especially as raw material, the extract can be taken
orally directly. As a result, the bioavailability of the extract can be
raised. Although explanatory embodiments have been shown and described,
it would be appreciated by those skilled in the art that changes,
alternatives, and modifications can be made in the embodiments without
departing from spirit and principles of the present invention. Such
changes, alternatives, and modifications all fall into the scope of the
claims and their equivalents. As a result, it is obvious that the
embodiments described herein are explanatory, illustrative, and used to
generally understand the present invention, which shall not be construed
to limit the present invention.