An interpretation was featured in the first episode of The Muppet Show, with Miss Piggy, four chickens, four frogs, and two males pigs being led by Kermit the Frog in the Muppet Glee Club, Miss Piggy sang a solo in the third verse until the end, her voice being performed by Richard Hunt instead of Frank Oz, her then-regular performer. In a later episode, three octopi played the song on the drums and kazoo. Animal took offense to their bad playing, and attacked them.

This song is currently played by "Ohio's Pride," The University of Akron Marching Band.

The Michigan Marching Band has been playing a version of Temptation arranged by Jerry Billik for over 40 years. It also plays a shortened version whenever an opponent is stopped on third down. It also plays the song, in full, during the post-game performance, followed by Hawaiian War Chant, because according to announcer Carl Grapentine, "you can't have one without the other." The same arrangement is also used by the University of Michigan athletic bands, including the hockey, women's volleyball, and men's and women's basketball bands.