This paper details the influence of the magnitude of imposed inflow fluctuations on Large Eddy Simulations of a spatially developing turbulent mixing layer originating from laminar boundary layers. The fluctuations are physically-correlated, and produced by an inflow generation technique. The imposed high-speed side boundary layer fluctuation magnitude is varied from a low-level, up to a magnitude sufficiently high that the boundary layer can be considered, in a mean sense, as nominally laminar. Cross-plane flow visualisation shows that each simulation contains streamwise vortices in the laminar and turbulent regions of the mixing layer. Statistical analysis of the secondary shear stress reveals that mixing layers originating from boundary layers with low-level fluctuations contain a spatially stationary streamwise structure. Increasing the high-speed side boundary layer fluctuation magnitude leads to a weakening of this stationary streamwise structure, or its removal from the flow entirely. The mixing layer growth rate reduces with increasing initial fluctuation level. These findings are discussed in terms of the available experimental data on mixing layers, and recommendations for both future experimental and numerical research into the mixing layer are made.

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