5′-Fluorescein Phosphoramidite is mostly used to label sequencing primers. The DMT group of the 6-Fluorescein phosphoramidite allows coupling quantification. A coupling of 15 minutes is recommended and we recommend to remove the DMT group before oligonucleotide purification. Due to the instability of the Fluorescein to light, we recommend to use the phosphoramidite immediately after the preparation of the solution. The 6-FAM Phosphoramidite has found applications in Genomic research, such as DNA sequencing and amplification. The product contains no DMT but is a single isomer of the 6-carboxyfluorescein with a 6 carbon linker. A coupling efficiency of more than 95% can be achieved resulting in a single fluorescein-oligonucleotide peak on reversed-phased purification. Use of methylamine should be avoided.

Technical Information

Molecular Formula: C68H79N4O12PS

Molecular Weight: 1 207.43 g/mol

Specification:

HPLC – Identity is confirmed by comparison with a reference sample, Minimum purity

NMR – Purity is determined by 31P NMR and isomeric purity is veified by 1H NMR