We believe this site might serve you best:

United States

Select a Different Country and Language

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Related Resources

Related Articles

Abstract

Single Step (KRX) Competent Cells are ideal for rapidly generating new clones for recombinant protein expression. We examined the effect of induction time and media on recombinant protein yield and activity in KRX. Firefly and Renilla luciferases were used as models. Induction times as brief as 4 hours were sufficient to produce significant quantities of active protein. Using Single Step (KRX) Competent Cells with this short induction protocol, protein can be expressed and analyzed in less time, without the need for overnight induction.

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.