Preseason game number two will bring more playing time for the starters. There are also prime opportunities for some other players looking to fill voids at positions where there are injuries.

1 - Anquan Boldin Boldin did not dress for the preseason opener and a lot has changed since last Thursday. Sammy Watkins was traded, Jordan Matthews was added, but he's not expected to play. Boldin must begin to establish on field chemistry with Tyrod Taylor knowing right now he's probably the quarterback's most trusted receiver.

2 - E.J. Gaines It's difficult to know just how much time Gaines will see knowing he's had all of three practices with his new club. But Gaines has turned in some plays in the final days of training camp and has been running with the second team. He figures to get significant snaps knowing the coaching staff has some catching up to do on him in order to make an accurate evaluation.

3 - Andre Holmes It's put up or shut up time for the tall, lanky receiver. Holmes has had trouble stringing positive days at camp together on offense. He's got to make some plays, especially with the door open at receiver with Watkins in L.A. and Matthews on the injured list.

4 - Matt Milano The rookie linebacker didn't have a fat stat line after the preseason opener, but his reads were pretty consistent and he was around the ball. Milano will need to take the next step and be in on more plays Thursday night against the Eagles. He should get opportunities as he plays in both the second team base defense and the second nickel package.

5 - Jordan Mills With training camp now shifting to One Bills Drive, Mills, to his credit, has not relinquished his grip on the starting right tackle spot. If he can put together a solid performance against the Eagles, especially in pass protection it'll go a long way to solidifying his starting role.