3
What Is PCR? PCR is a method for amplifying DNA. It requires the following components: –Primers---determine the sequence to be amplified –DNA Polymerase ---Taq polymerase, stable at high temperatures required by the reaction –Nucleotides –Ions, such as magnesium chloride –Target sequence (supplied by your cheek cells)

5
D1S80 VNTR amplified by PCR VNTR= variable number of tandem repeats D1S80 –Found on Chromosome 1 –Contains 16 nucleotide sequence repeated times –Most individuals are heterozygous, having different numbers of repeats in each of their two D1S80 loci

9
Polymerase Chain Reaction Place your PCR tube in the Thermal Cycler It is programmed for 32 cycles of 94 o C, 65 o C, 72 o C for 30 seconds each. After PCR, samples will be stored in the freezer until the next laboratory period.