f4: MGMT is a downstream target of ERp29.ERp29-transfected cells (cone B) (a) or MCF7 cells (b) were treated with ERp29 siRNA (#1) or MGMT siRNA (#3) or control siRNA and the expression of ERp29 and MGMT was analysed. ERp29 knockdown decreased the expression of MGMT whereas MGMT knockdown was unable to decrease the level of ERp29 in both clone B cells (a) and MCF-7 cells (b). **p < 0.01, ***p < 0.001, relative to control.

Mentions:
To further understand whether MGMT is a downstream target of ERp29, the MB-231/ERp29 cells (clone B) were respectively treated for 48 h with MGMT siRNA, or ERp29 siRNA, or the non-targeted control siRNA. We showed that depletion of ERp29 in MB-231/ERp29 cells reduced the level of MGMT compared to those treated with control siRNA (Fig. 4a). However, depletion of MGMT was unable to affect the level of total ERp29 (endogenously and exogenously expressed) in these cells (Fig. 4a). This is reflected by the fact that the overall ERp29 level in the MGMT siRNA-treated MB-231/ERp29 cells was similar to that expressed in the untreated or control siRNA-treated MB-231/ERp29 cells.

f4: MGMT is a downstream target of ERp29.ERp29-transfected cells (cone B) (a) or MCF7 cells (b) were treated with ERp29 siRNA (#1) or MGMT siRNA (#3) or control siRNA and the expression of ERp29 and MGMT was analysed. ERp29 knockdown decreased the expression of MGMT whereas MGMT knockdown was unable to decrease the level of ERp29 in both clone B cells (a) and MCF-7 cells (b). **p < 0.01, ***p < 0.001, relative to control.

Mentions:
To further understand whether MGMT is a downstream target of ERp29, the MB-231/ERp29 cells (clone B) were respectively treated for 48 h with MGMT siRNA, or ERp29 siRNA, or the non-targeted control siRNA. We showed that depletion of ERp29 in MB-231/ERp29 cells reduced the level of MGMT compared to those treated with control siRNA (Fig. 4a). However, depletion of MGMT was unable to affect the level of total ERp29 (endogenously and exogenously expressed) in these cells (Fig. 4a). This is reflected by the fact that the overall ERp29 level in the MGMT siRNA-treated MB-231/ERp29 cells was similar to that expressed in the untreated or control siRNA-treated MB-231/ERp29 cells.