Galactose dehydrogenase from recombinant E. coli

Cat No.

NATE-1931

Description

In enzymology, a galactose 1-dehydrogenase (EC 1.1.1.48) is an enzyme that catalyzes the chemical reaction: D-galactose + NAD+ rightleftharpoons D-galactono-1,4-lactone + NADH + H+. Thus, the two substrates of this enzyme are D-galactose and NAD+, whereas its 3 products are D-galactono-1,4-lactone, NADH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. This enzyme participates in galactose metabolism.

Source

E. coli

Form

Ammonium sulphate suspension

Enzyme Commission Number

EC 1.1.1.48

Activity

more than 80 U/mg protein

CAS No.

9028-54-0

Molecular Weight

ca. 33,800

pH Stability

5.0 - 10.0

Unit Definition

One unit of activity is defined as the amount of GalDH that forms 1 μmol of NADH per minute at 30 °C.

Optimum pH

10.5

Thermal stability

No significant decrease in activity up to 50 °C with Ammonium sulphate and 40 °C without Ammonium sulphate.