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Abstract

Ultrahigh-resolution adaptive optics–optical coherence tomography (UHR-AO-OCT) instrumentation allowing monochromatic and chromatic aberration correction was used for volumetric in vivo retinal imaging of various retinal structures including the macula and optic nerve head (ONH). Novel visualization methods that simplify AO-OCT data viewing are presented, and include co-registration of AO-OCT volumes with fundus photography and stitching of multiple AO-OCT sub-volumes to create a large field of view (FOV) high-resolution volume. Additionally, we explored the utility of Interactive Science Publishing by linking all presented AO-OCT datasets with the OSA ISP software.

Figures (8)

An example of AO-OCT scanning areas used for creating larger FOV volume (based on the acquisition of nine sub-volumes). Numbers on the sub-volumes refer to the order in which they were acquired. Fundus photo is used for reference.

UHR-AO-OCT volume of the retinal structures acquired over 0.25×0.3 mm area on the 4.5 deg temporal retina (TR). Top row: left, visualization of the data acquired with UHR-AOOCT system focus set on photoreceptor layers; center, fundus photo with marked location of the acquired volumes; right, visualization of the data acquired with UHR-AO-OCT system focus set on inner retinal layers. Bottom row: left, screenshot from OSA ISP with the UHRAO-OCT volume with focus set on photoreceptor layers (View 1); right, screenshot from OSA ISP with the UHR-AO-OCT volume and focus set on inner retina layers (View 2). Multiple microscopic and cellular structures can be recognized clearly (including photoreceptors seen on the lower left panel and microcapillaries seen on the lower right panel).

Datasets

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