Abstract

The aim of this study was to bacteria isolation and characterization proteases from water swamp. The water swamp samples collected fromIndralaya for microbial isolation. Three isolates showed proteolytic index >1.00. The optimum pH of extraceluller proteases from A6S3,A4S3 and A15S3 were 7.5; 8.0; 8.0, respectively. The optimum temperature of A6S3, A4S3 and A15S3 protease were 40; 50; 50 oC,respectively. Effect of metal ion (Fe, K, Mn, and Zn) shown Fe and K were inhibit protease A6S3, all metal ion were inhibit protease A4S3and K, Mn and Zn inhibit protease A15S3. Study on the effect of metals ion and spesific inhibitors indicated that all protease weremetaloprotease. Their moleculer weights were determined by using SDS-PAGE and zymogram technique for A6S3 isolate were 70, 88, 106and 121 kD respectively. Whereas for isolate A4S3 was 138 kD and isolate A15S3 was 131 kD.

Abstract

The aim of this research is find out the lactic acid bacteria (LAB) from peda, bekasam, terasi and rusip which had antagonis by Morganella morganii (histamin producer bacteria). The result was that the number isolate from peda was 3 (1P1 104, 3P1 104, 2P2 104), bekasam was 7 (1B1 104, 2B1104, 1B2104, 2B2104, 3B2 104, 1B1 105, 2B1105), terasi was 4 (1T1 104, 2T1 104, 3T1 104) and rusip was 5 isolates (1R1103, 2R1103, 3R1103, 1R1104,Â 1R2104). All the isolates was bacill, gram positive, and negative catalase. The isolate which had biggest of clear zone was 3B1104 and 1T11104, with the diameter of clear zone was 1.725 cm and 1.300 cm. Keywords: Antagonis, Lactic acid bacteria, Morganella morganii

Abstract

This study aims to determine whether tempeh from lotus seeds can be accepted by panellist through hedonic tests and hedonic quality. This research method uses a randomized block design (RCBD) with different proportions of lotus seeds as a treatment and repeated 3 times. Treatment with different proportions of lotus seeds is A0 (100% soybean seeds), A1 (50% soybean seeds-50% lotus seeds) and A2 (100% lotus seeds). The parameters of this study include sensory analysis of hedonic and hedonic quality (appearance, color, texture, aroma and taste). The hedonic quality sensory results significantly affect the appearance, texture, aroma and color while the hedonic effect significantly on the color but not significantly affect the taste, texture and aroma parameters. Tempe with the proportion of lotus seeds were as much as 50% better than 100% lotus seed tempeh, both of the characteristics of the quality of hedonic and hedonic.

Abstract

The objective of this research was to know effect of solvent (NaHCO3 0,5% and MgCO3 0,03%) and Tween 80 (0,5%, 1%, and 1,5%) to obtain the chlorophyll and phytochemical compounds of kiambang leaves. The research used factorial randomized block design with two treatments and each treatment was replicated three times. The treatments wereÂ the type of solvent (NaHCO3 0.5% and MgCO3 0.03%) and the concentration of Tween 80 (0.5%, 1% and 1.5%). The parameters of research were total chlorophyll content, water dissolve chlorophyll, phytochemichal, colour intensity (Hunter a, Hunter b, and Lightness), and colour stability. The result showed that the average of total chlorophyll ranges from 10.261 mg/L to 16.435 mg/L, water dissolve chlorophyll ranges from 4.503 mg/L to 5.739 mg/L, Hunter a ranges from -2.8 to -4.667, Hunter b ranges from +6.1 to +14.6, Lightness ranges from 29.4 to 34.067. Phytochemical compounds of kiambang leaves were alkaloid, fenol, and saponin. The colour stability test of kiambang leaves by using NaHCO3 0.5% and Tween 80 1.5% indicated the best colour stability during four weeks storage in aerobic condition. Â Keyword: kiambang extract, chlorophyll, phytochemical

Abstract

The purpose of this research was to be know antioxidant activity, anticolestrol and antihypertence of extract rusip. This research was conducted from May 2017 until October 2017 using experimental laboratory method and data analysis was done descriptively. The research stages included measurement of pH, salt content analysis, determination of protein content, determination of peptides, antioxidants testing with the ABTS method, the analysis of ACE inhibitors (Angiotensin Converting Enzyme) and analysis of HMG-CoA reductase inhibitors. The results showed that there are three types of rusip which has the complete label is rusip A, B and C. The pH value rusip ranged from 5.85 to 6.01. The salt contained in rusip ranged from 13.94 to 15.68%. Rusip protein content ranged from 14.71 to 18.39 mg / mL. The content of yield rusip extract ranged from 2.26 to 3.32 % and the content of peptide extract ranged from 13.05 to 14.80%. Rusip is a highly protein-rich food and contains many peptides. The antioxidant activity of rusip extract and activity of HMG-CoA inhibitor of russip extract are low. Rusip extract has a high activity as an ACE inhibitor (Angiotensin-I Converting Enzyme) of 95.75%.

Abstract

ABSTRACTÂ Â Â Â Â Â Â Â Â Â Â Â Mahakamâs bridge was a bridge that connected Samarinda with Samarinda Seberang and other cities like Balikpapan, Kukar and Kubar. Along with the rapid of population growth and economic in Samarinda gave an impact to the user of traffic to the bridge over the Mahakam that connected Samarindaâs city and Samarinda Seberang with the inter-city and other areas. As the reality that happened in Samarinda, in the afternoon and holidays the traffic to Mahakamâs bridge and otherwise always jammed with vehicle till made a severe traffic jammed in the second pathway of Mahakamâs bridge.Â Â Â Â Â Â Â Â Â Â Â Â Â Â According to the problems above, the government of Kalimantan Timur took a wise solution with made an initiative to build a same bridge in the left of Mahakam bridges. With the construction of the twin bridges, the writers interested to know how the responsibility of the government partners in the construction of the twin bridges.Â Â Â Â Â Â Â Â Â Â Â This research method that used in this law script is descriptive design where the writer described the reality of the twin bridges construction and customized with data from Dinas Pekerjaan Umum Bidang Bina Marga Kementerian Pekerjaan Umum.Â Â .Â Â Â Â Â Â Â Â Â Â Â The legal standings that used in construction ofÂ the twins bridges in Samarinda is the governor rule of Kalimantan Timur Nomor 38 Tahun 2012,Â from the governor rule of Kalimantan Timur all the rules that became the legal standings in construction of the twins bridges so have a legally enforceable.Â Â Â Â Â Â Â Â Â Â Â Based on the condition of twinâs bridges construction today, the process of building Twins Bridge is foundation construction stage. The foundation of Twins Bridge could be seen by the people when they passed through the twin bridge.Â Â Â Â Â Â Â Â Â Â Â After the writer conducted research in Dinas Pekerjaan Umum Bidang Bina Marga Kementerian Pekerjaan Umum the writer concluded that the responsibility of government partner in twins bridge construction based on the contract between the government of Kalimantan Timur with the government partners is the Contractor, PT. PP Perdana KSO, Consultant Planner is PT. Perencana Jaya and the supervising consultants is PT. Pelaksana Desain Karya Cipta.Â Â Â Â Â Â Â Â Â Â Â Keywords : (The Twins Bridge)