Mammalian Two-Hybrid System

The Matchmaker Mammalian Two-Hybrid Assay Kit 2 allows rapid and convenient analysis of protein-protein interactions in transfected mammalian cells. The kit uses a SEAP secreted reporter to measure the interactions between the two proteins of interest, eliminating the need for cell lysis.

Because these two-hybrid assays are performed in mammalian cells, proteins encoded by mammalian cDNAs are more likely to be in their native conformation; therefore, post-translational modifications and experimental results are more likely to represent biologically significant interactions.

The Matchmaker Mammalian Two-Hybrid Assay Kit 2 is based on the same principle as the Matchmaker Gold Two-Hybrid System—two fusion proteins are expressed that contain either a DNA binding domain or an activation domain. If the proteins interact, transcription of the SEAP reporter gene is activated from the pG5-SEAP plasmid.

Features

Applications

Confirm protein-protein interactions in mammalian cells

Map interacting domains

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.