The EZ1 RNA Tissue Mini Kit contains all required reagents and labware for automated purification of total RNA from up to 10 mg standard tissue samples using magnetic particle technology. Reagents are supplied in prefilled reagent cartridges, which ensures speed and convenience in loading the instrument. The EZ1 RNA Tissue Kit can be used with the EZ1 Advanced XL, the EZ1 Advanced, or the BioRobot EZ1.

Automated purification with the EZ1 RNA Tissue Mini Kit requires use of the EZ1 Advanced XL and the EZ1 Advanced XL RNA Card, or the EZ1 Advanced and the EZ1 Advanced RNA Card, or the BioRobot EZ1 and the EZ1 RNA Card. EZ1 cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure.

Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1-14 samples (EZ1 Advanced XL) or 1-6 samples (EZ1 Advanced and BioRobot EZ1) in a single run. Presealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination or degradation due to RNases. The automated procedure provides high repeatability and linear yields over a range of starting amounts. The flexibility to purify high-quality total RNA from a wide range of tissue types ensures that samples can be purified for a range of gene expression analysis applications. RNA yield using the EZ1 RNA Tissue Mini Kit is dependent upon many variables, including sample weight, tissue type and developmental state.

Procedure

Tissues are first lysed and homogenized in Buffer RLT. RNA is then purified in one step on the instrument following the automated EZ1 RNA procedure. For certain RNA applications that are sensitive to very small amounts of DNA, an integrated DNase digestion step removes genomic DNA contamination. Total RNA is eluted in 200 µl, 100 µl, or 50 µl, ready to use in all downstream applications.

Applications

The high-quality RNA obtained using the EZ1 RNA Tissue Mini Kit is suited for use in many applications, such as:

EZ1 RNA Cell Mini Kit
For purification of total RNA from human and animal cells using EZ1instruments
EZ1 RNA Tissue Mini Kit
For purification of total RNA from easy-to-lyse human and animal tissues using EZ1 instruments
EZ1 RNA Universal Tissue Kit
For purification of total RNA from any type of human and animal tissue using EZ1 instruments