The MagAttract Magnetic Rack is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Performance

The MagAttract Magnetic Rack enables separation of high-molecular-weight DNA from different sample types. Convenience is assured as the hands-on time required for processing is reduced compared to other existing magnetic bead or spin-column-based purification methods. Up to 12 samples can be handled in parallel by easily inserting the 2 ml sample tubes into the tube holder of the MagAttract Magnetic Rack.

Principle

The MagAttract Magnetic Rack is a convenient separation device for nucleic acid purification using magnetic beads (e.g., MagAttract Suspension G) in 2 ml tubes. The beads are pulled to the side of the 2 ml tube by a magnetic field, which has a strength of >50 megaoersted, and are held in place while solutions are exchanged or removed. Removal of the magnetic field allows easy resuspension of the magnetic particles.

Procedure

The MagAttract Magnetic Rack consists of 3 separate components in total: a tube holder, a magnetic base, and an adapter plate. The MagAttract Magnetic Rack is designed for handling up to 12 samples in parallel in 2 ml reaction tubes. The magnetic bead-based purification is performed on a standard laboratory shaker (e.g., Eppendorf Thermomixer) suited for the shaking of reaction tubes, as well as microtiter plates in SBS format. The tube holder of the MagAttract Magnetic Rack is fixed onto the shaker by the SBS adapter plate. The 2 ml reaction tubes are plugged into the tube holder of the MagAttract Magnetic Rack. Up to 12 sample tubes fixed to the tube holder are then transferred in a single step to a 24-well block of the shaker. The tube holder with 2 ml sample tubes perfectly fits onto, for example, the Eppendorf Thermomixer 24-well block for 1.5 ml tubes. To use a shaker suited for the shaking of microtiter plates in SBS format, the adapter plate of the MagAttract Magnetic Rack has to be fixed onto the shaker first. The tube holder of the MagAttract Magnetic Rack (with up to 12 reaction tubes) is then attached to adapter plate of the MagAttract Magnetic Rack. The magnetic beads are easily separated by removing the tube holder from the shaker and placing it onto the magnetic base. Magnetic beads are separated by strong magnets in <1 minute. Supernatants can be easily removed by pipetting.

Applications

The MagAttract Magnetic Rack can be used to conveniently process the MagAttract HMW DNA Kit for isolation of high-molecular-weight genomic DNA.