Tuesday, April 30, 2013

Dual luciferase assays have become the standard assay when
information on the effect of regulatory elements and proteins with regard to
regulation of gene expression is desired. Dual luciferase assays use one
reporter to measure the test conditions while the second serves as a control
for transfection efficiency and cell viability. Typically this has employed
sequential activation of test and control with requisite quenching.

The use of spectrally resolved forms of luciferase allow for
the detection of two luciferases in one sample simultaneously without
quenching. The POLARstar Omega from BMG LABTECH is ideally suited for
monitoring activity from two luciferases in a spectrally resolved manner. The
performance of the POLARstar Omega using the Thermo Scientific Pierce Dual
Spectral Luciferase assay is described in application note 233