Introduction: Uterine Prolapse (UP) is the contributor to reproductive health problems that influence the women’s quality of life. It is the most frequent cause of gynaecological morbidities among women in India and major indication for hysterectomy.

Aim: To evaluate the effectiveness of Structured Teaching Programme (STP) on knowledge regarding preventive measures of UP among mothers and to find out the association between knowledge of mothers regarding preventive measures of UP and selected demographic variables.

Materials and Methods: A quantitative research approach with pre-experimental one group pre-test and post-test design was used for the study. The setting of the study was Gynaecology OPD at AIMS, Kochi. By using non-probability convenience sampling technique, 40 antenatal mothers were selected. A semi-structured questionnaire was used to assess the demographic data and knowledge regarding preventive measures of the UP in mothers. The STP was provided to the participants after pre-test. The post-test was done on the 14th day after pre-test. The sample characteristics were described using frequency, percentage. Chi–square test was used to find out the association between knowledge of mothers regarding preventive measures of UP and selected demographic variables.

Results: The mean pre-test knowledge score was 11.85±4.36 and the mean post-test knowledge score was 19.75±2.98. There was a statistically significant improvement in the level of knowledge regarding preventive measures of UP among the mothers (t-value=16.01, p<0.05) and significant association was found between Pre-test level of knowledge and age of mothers (?2=6.30,p=0.043). However, no significant association was found between pre-test level of knowledge and selected demographic and clinical variable which includes education, occupation, place of residence, previous information about UP etc.

Conclusion: The findings of the study showed a significant improvement in knowledge regarding preventive measures of UP among mothers. The STP was effective and it is also suggested that the programme should be continued in order to uplift the overall health and practices of mothers. Hence, it will help to reduce the incidence of UP and enhance the quality of life in future.