M3887-53H reacts with the I-Aq MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2q bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. M3887-53H crossreacts with I-As and I-Av; reactivity with other haplotypes has not been reported.

Catalog #

M3887-53H

Applications

Suitable for use in Immunoprecipitation, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution

Flow Cytometry: 1ug/10e6 cells in 100ul volume

Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Clone Type

Monoclonal

Isotype

IgG2b,k

Clone No

KH116

Host

Mouse

Source

Mouse

Concentration

~0.1mg/ml

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity

Purified by immunoaffinity chromatography.

Immunogen

B10.Q mouse splenocytes

Specificity

Recognizes mouse q haplotype, I-As and I-Av haplotypes.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.