paraffin for processing mice parts

From:

"DiCarlo, Margaret" (by way of Histonet)

Histonetters,
I have the opportunity to use a tissue processor to process decaled mice
forearms and hind legs. (Presently I've been manually processing which takes
much longer.) However, I must use a combined paraplast and paraplast extra
in the tissue processor. After 3 changes of this combined paraffin should I
put the tissue in a fourth paraffin of Tissue Prep II in a vacuum oven for
another two hours or can I just embed but embed with the Tissue Prep II ?
Will using different paraffins have any effect on cutting the tissue and
doing future immuno's? I would appreciate any advice.
Peggy DiCarlo HT (ASCP)
Orthopedics Bone Lab
Buffalo General Hospital
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