Protein equivalent aliquots of cell lysates made by a detergent free
lysis method from each time point were subjected to western blot
analysis using anti-β-catenin and anti-GSK3ab antibodies.
β-catenin/GSK3a or GSK3b level of expression after HGF treatment was
quaantified by densitometry. Bars indicate the mean and standard
deviation of three independent experiments. Asterisk indicates level of
statistical significance for p<0.05.