Note: The primers were repeated using Whitbeck's method.(PMID:2841484) PCR was carried out according to the following protocol: 1mM P1, 1mM P2, 1microL DNA BHV-1(phenol-chloroform extraction from virions of BHV-1, titer 10^7 TCID50/ml) and 0,5,10,20 microL glycerol diluted with water 1:1 total reaction volume 50microL were added. Mixture was heated to 100C 8min, then chilled in ice before PCR.