Summary

Secondary Antibodies

Negative Isotype Controls

Useful Reagents

Positive Controls

Histology Controls

More Images

References

Reviews

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Mouse anti Human ankyrin G antibody, clone 463 recognizes ankyrin G and reacts with a region within the spectrin binding domain. In Western blotting it detects the 190 kDa isoform.

Ankyrins are a family of spectrin-binding protein that couple the spectrin/actin cytoskeletal network to the cytoplasmic domains of integral membrane proteins which include channels such as the anion exchanger, voltage-dependent sodium channel and the Na/K ATPase. 3 forms of ankyrin G exists, 190 kDa, 270 kDa and 480 kDa, that are localized in the axonal intial segments and the nodes of Ranvier of myelinated axons where fluxes of action potential occur and ion channels are enriched.

Intended Use

Target Species

Human

Species Cross-Reactivity

Target Species

Cross Reactivity

Rat

Mouse

Dog

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Reconstitution

Preparation

Preservative Stabilisers

0.09%

Sodium Azide (NaN3)

0.2%

Gelatin

Immunogen

A synthetic peptide corresponding to amino acids from the spectrin-binding domain of human ankyrin G.

Purity

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Reagents In The Kit

Preparing The Antibody

Test Principle

Buffer Solution

Phosphate buffered saline

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the P3X63/Ag 8.653 mouse myeloma cell line

Storage

Store at +4oC or at -20oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name

Yes

No

Min Dilution

Max Dilution

Immunofluorescence

1-2 µg/ml

Immunohistology - Frozen

1-2 µg/ml

Western Blotting

2-5 µg/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.