Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

WB

1/2000. Predicted molecular weight: 43 kDa.

IHC-P

1/50.

Target

FunctionComponent of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

Sequence similaritiesBelongs to the rad9 family.

Post-translationalmodificationsConstitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

AB70810 DISCOUNT CODE: XXXX Expiration date: AB117848 DISCOUNT CODE: XXXX Expiration date: I am very pleased to hear you would like to accept our offer and test both ab70810 and ab117848 and in ChIP. Above are the code that I have created for each...

Thank you for contacting us. While we do not have a ChIP graded RAD9A antibody. It is highly likely that the products we do have may work in your experiment. Ideally, specific antibodies for ChIP should be affinity-purified. Polyclonal antibodies are...