Sample Analysis Design. Element2 - Basic Software Concepts

Transcription

1 Sample Analysis Design Element2 - Basic Software Concepts

2

3

4 Scan Modes Magnetic Scan (BScan): the electric field is kept constant and the magnetic field is varied as a function of time the BScan is suitable for scanning large, continuous mass ranges to obtain a complete spectrum from an unknown sample. for repeated scanning, a fast flight-back follows each scan depending on the (mass) distance for the flight-back, a settling time for the magnet must be allowed the settling time takes into account the time needed for the magnet to recover from the fast flight-back of each cycle this scan mode is also used for Mass Calibration in order to account for the non-linear behavior of the magnet

5 Scan Modes Electric Scan (EScan): the magnetic field is kept constant and scanning is performed electrically by varying the accelerating voltage and the ESA high voltage this scan mode is the fastest scan the EScan starts with setting the magnet to a mass at the lower end of the Mass Range the available mass range for the electric scan depends on the magnet mass; it ranges from -6% to +30% of the magnet mass

6 Scan Modes EScan advantages: fast scans can be conducted over the detectable mass range without huge energy transfer in the magnet = this results in settling times that do not have to be observed even when performing the fastest repeated scans thus possible to scan the mass ranges of the isotopic pattern and to skip the ranges of no interest possible to carry out replicate measurements in quick succession because of the low energy transfer the advantages of EScan make it the preferred and default scan mode

7

8

9 Integration Window (%): Expressed as a percentage of the peak width, is used to calculate peak intensity. A value of 100% means that the intensity will be calculated from samples, measured in a window of ± ½ peak width of peak center Peak Search Window (%): This specifies the mass window, where the software performs a search (calculation) for the peak center. For example, a value of 100% means that all samples are used in a window of ± ½ peak width of the accurate mass as specified during the elements/isotope selection.

10 Samples per Peak In order to establish a minimum level of precision, the ion signal (peak) must be measured several times during the scan

11 Samples per Peak Recommended values for Samples per Peak as a function of the mode of resolution: -10 samples for low resolution measurements - 20 samples for medium resolution measurements - 30 samples for high resolution measurements

12 Detection Modes

13 Detector Modes Counting Mode: is a digital measurement (counting) and counts events instead of ion signal height it is therefore very sensitive and useful especially for the detection of low signals (concentrations). during acquisition, the number of occurrences is used to generate the intensity information that is stored in a data file this mode of operation can be used in the detection range from 0 to 5 x 10 6 counts per second (cps)

14 Detection Modes Analog Mode: is a standard detection mode each time an ion signal exceeds a certain threshold, the height above the threshold delivers the intensity information (that is stored in a data file) the detection range is between 10 4 and 10 8 cps

15 Detection Modes Both Mode: the analog and the counting signals are monitored by the hardware continuously should the ion signal exceed the security threshold ( trip ) of the ion counter (5 x 10 6 cps), then the protection device disables the counting part until the intensities are below the trip level (if applicable) Note a cross calibration between modes is done automatically

16 Element2 Software Concepts MASS CALIBRATION

17 Mass Calibration The expectation of an ICP-MS user is to see the exact information about peaks, mass numbers, etc. when looking at the computer screen Unfortunately, the computer hardware has no idea about mass and intensity of ions or peaks in a mass spectra

18 Mass Calibration The computer knows DAC (Digital-to-Analog Converter) steps for setting the magnetic field of the instrument and the ADC steps (Analog-to- Digital Converter) for a received intensity The Mass Calibration program translates and adjusts the information available by the computer hardware into the information an ICP- MS user really needs; i.e. mass numbers and intensities for single ions and isotopic patterns

19 Mass Calibration The computer translates DAC values into mass numbers. The DAC values are used for setting the strength of the magnetic field, which modifies the direction of the ions. The hardware is referred to as Magnet DAC or MDAC The actual translation table, the Mass Calibration Table, is stored on the computer s disk in the data directory as <name>.mcl

4.3 Analog-to-Digital Conversion overview including timing considerations block diagram of a device using a DAC and comparator example of a digitized spectrum number of data points required to describe

Setting up a Quantitative Analysis MS ChemStation Getting Ready 1. Use the tutorial section "Quant Reports" on the MSD Reference Collection CD-ROM that came with your ChemStation software. 2. Know what

Chapter 6: From Digital-to-Analog and Back Again Overview Often the information you want to capture in an experiment originates in the laboratory as an analog voltage or a current. Sometimes you want to

In-Depth Qualitative Analysis of Complex Proteomic Samples Using High Quality MS/MS at Fast Acquisition Rates Using the Explore Workflow on the AB SCIEX TripleTOF 5600 System A major challenge in proteomics

Chapter 4 Calibration The accurate calibration of all detectors is crucial for the subsequent data analysis. The stability of the gain and offset for energy and time calibration of all detectors involved

Pesticide Analysis by Mass Spectrometry Purpose: The purpose of this assignment is to introduce concepts of mass spectrometry (MS) as they pertain to the qualitative and quantitative analysis of organochlorine

What is ICP-MS? and more importantly, what can it do? Inductively Coupled Plasma Mass Spectrometry or ICP-MS is an analytical technique used for elemental determinations. The technique was commercially

ToF Mass Calibration Essentially, the relationship between the mass m of an ion and the time taken for the ion of a given charge to travel a fixed distance is quadratic in the flight time t. For an ideal

35'th Annual Precise Time and Time Interval (PTTI) Systems and Applications Meeting San Diego, December 2-4, 2003 A PC-BASED TIME INTERVAL COUNTER WITH 200 PS RESOLUTION Józef Kalisz and Ryszard Szplet

13 C NMR Spectroscopy Introduction Nuclear magnetic resonance spectroscopy (NMR) is the most powerful tool available for structural determination. A nucleus with an odd number of protons, an odd number

Students Manual for the Exam General Engineering and Electrical Civil Engineering Discipline -- October March 2014 2013 -- COPYRIGHT NOTICE COPYRIGHTS 2013 NATIONAL CENTER FOR ASSESSMENT IN HIGHER EDUCATION

Limits to Detection/Measurement Ionization is fundamentally a Probabilistic Process Just like radioactive decay So is transmission through the analyzer There is an intrinsic statistical uncertainty Proportional

E.d.a.s.VX Data acquisition on board road and track vehicles The E.d.a.s.VX System is designed for portable applications running on 12 Volts DC, and is capable of measuring at selectable rates up to 30,000,000

Specifications MassHunter WorkStation for Agilent 7700 Series ICP-MS Specifications: MassHunter Workstation (G7211A) for the Agilent 7700 Series The ICP-MS MassHunter Workstation for the Agilent 7700 Series

Unique Software Tools to Enable Quick Screening and Identification of Residues and Contaminants in Food Samples using Accurate Mass LC-MS/MS Using PeakView Software with the XIC Manager to Get the Answers

Moving Magnet Actuator MI FFA series The moving magnet MI-FFA series actuators are a line of actuators designed to be a true alternative for pneumatic cylinders. The actuators incorporate an ISO 6432 interface

LC-MS/MS for Chromatographers An introduction to the use of LC-MS/MS, with an emphasis on the analysis of drugs in biological matrices LC-MS/MS for Chromatographers An introduction to the use of LC-MS/MS,

2015 Page 2 Waters Core Chromatography Training (2 Days) The learning objective of this two day course is to teach the user core chromatography, system and software fundamentals in an outcomes based approach.

Longitudinal Relaxation Time (T 1 ) Measurement for 1 H on VnmrJ2.2D (use on Ra, Mut, and Isis) Knowledge of the T 1 's of a molecule is important in the setup of many 1D and 2D experiments, in which the

Measuring Resistance Using Digital I/O Using a Microcontroller for Measuring Resistance Without using an ADC. Copyright 2011 John Main http://www.best-microcontroller-projects.com Page 1 of 10 Table of

Audit Report and Requirements of Mantid Software from the Molecular Spectroscopy Group 1. Current Status (2013) The current status of the data analysis software, Mantid, corresponding to each group instrument

Digital to Analog Conversion Using Pulse Width Modulation Samer El-Haj-Mahmoud Electronics Engineering Technology Program Texas A&M University Instructor s Portion Summary The purpose of this lab is to

Time-of-Flight Mass Spectrometry Technical Overview Introduction Time-of-flight mass spectrometry (TOF MS) was developed in the late 1940 s, but until the 1990 s its popularity was limited. Recent improvements

1 CIRCUIT DESIGN If not using one of First Sensors ZBXYA interface boards for sensor control and conditioning, this section describes the basic building blocks required to create an interface circuit Before

For more Project details visit: http://www.projectsof8051.com/android-based-alcohol-detection-system-usingbluetooth-technology/ Code 1435 Project Title Android based Alcohol detection system using Bluetooth

Application Note AN601 Tire pressure monitoring 1 Purpose This document is intended to give hints on how to use the Intersema pressure sensors in a low cost tire pressure monitoring system (TPMS). 2 Introduction

3 SOFTWARE AND PROGRAMMING LANGUAGES 3.1 INTRODUCTION In the previous lesson we discussed about the different parts and configurations of computer. It has been mentioned that programs or instructions have

Atoms, Molecules and Stoichiometry I. The Atomic Structure A. The Nucleus (a) Rutherford Bombardment Experiment This experiment leaded to the proposal of nucleus in atom. RESULTS (1)Most of the particles

1 Manual for simulation of EB processing Software ModeRTL How to get results. Software ModeRTL. Software ModeRTL consists of five thematic modules and service blocks. (See Fig.1). Analytic module is intended

Analyst 1.6 Software Getting Started Guide Release Date: August 2011 This document is provided to customers who have purchased AB SCIEX equipment to use in the operation of such AB SCIEX equipment. This

MassLynx 4.1 Getting Started Guide 71500113203/Revision A Copyright Waters Corporation 2005. All rights reserved. Copyright notice 2005 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA AND IRELAND.

Appendix 5 Overview of requirements in English This document is a translation of Appendix 4 (Bilag 4) section 2. This translation is meant as a service for the bidder and in case of any differences between

Transmitter Interface Program Operational Manual Version 3.0.4 1 Overview The transmitter interface software allows you to adjust configuration settings of your Max solid state transmitters. The following