Oxidize gtp 3 pyruvate is oxidized to acetyl group

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Unformatted text preview: of thioester bond
the cycle. oxidize GTP 3 pyruvate is oxidized to acetyl group. oxidize This page will be given 4 you
to
on your midterm and final. transient intermediate
decarboxylate main thing=making reduced cofactors under some circumstances you couldn't get cycle to work but when you add oxaloacetate labeled Why this baroque mechanism? Aconitase Goal: capture energy from: acetyl assymetrical
molecule 2 C02 1. One GTP made: equivalent to one ATP GTP + ADP nucleoside
diphosphate
kinase any time you
go through a
symmetric intermediate
the radio label
gets scrambled GDP + ATP 2. Lots of reduced cofactors produced. Their reducing power can be converted to ATP. one half dagger at eact position
becase the molecule is symmetrical Isotopic labeling experiments. •Synthesize an intermediate labeled with 14C at a specific position •Add to cell extract, isolate cycle intermediates, analyze for label. •See figure for fate of carbons * and † none are radiolabeled •isotope experiments
appeared to show the cycle as
drawn cou...
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