1b.Approach (from AD-416):
We previously conducted a study with Bing sweet cherries and published the results in the Journal of Nutrition in 2006. Because of a tight budget we could perform only a limited number of analyses at that time. Newer and more sensitive assays have become available since the time this study was conducted. We have plasma and white blood cell culture media samples obtained from our previous cherry study. We have recently procured the analysis of plasma samples from Rule Based Medicine for 89 antigens, by using the Human MAP Version 1.6. Initial analysis of these data shows that consumption of cherries altered the concentrations of several other markers. These results are very encouraging but need to be confirmed in our laboratory before we publish them. We will analyze the plasma samples for these select analytes by using the Meso Scale Diagnostic Technology in the Bioanalytical Support Laboratory at WHNRC. Our first priority is to complete the analysis of the plasma samples, if there are any funds left after that, we will analyze the cell culture media for select inflammatory markers. Results will be analyzed and findings published in a peer reviewed journal. Documents Trust with California Cherry Advisory Board. Log 38072.

3.Progress Report:

This is the final report for this project, which terminated in October of 2013. This research relates to objective 1 of the inhouse project, “Determine the effects of citrus liminoid glucoside (LG) on risk factors for cardiovascular disease including blood lipids and markers of inflammation in hypercholesterolemic humans". The overall goal of the inhouse project is to investigate the role of dietary fatty acids and citrus limonoid glucosides (LG) on chronic inflammatory diseases including non-alcoholic fatty liver disease, insulin resistance, diabetes, and cardiovascular disease. Cherries are rich in polyphenols and have strong antioxidant and anti-inflammatory effects as LG. Thus, consumption of cherries can affect the risk for the diseases listed above. By using a proteomics array approach, we analyzed human plasma samples from our earlier cherry study for biomarkers of chronic inflammatory diseases. We analyzed the data, prepared and submitted a paper to the Journal of Nutrition. Consumption of sweet Bing cherries reduced the plasma concentrations of several markers of inflammation, arthritis, diabetes, hypertension, cardiovascular disease, and cancer. Thus, our results suggest that cherry consumption selectively reduced several biomarkers associated with inflammatory diseases.