We shall discuss our recently published data together with unpublished stuffs highlighting a role of insulator proteins and their co-factors in functional long-range contacts. Our systematic analyses revealed hidden information in ChIP-Seq that may be used to predict long-range contacts in chromosomes. Further mathematical 'aggregation' of genome-wide Hi-C data confirms the relevance of such predicted long-range contact maps, defining a potent tool for determining contacts at a high resolution (< 500 bp). Such approach is currently being adapted for a broad usage by the research community of this tool as a package.

We will also present our published and unpublished results highlighting a role of the Histone-methyl transferase Mes4/NSD and dHypb/Set2 in transcription-dependent nucleosome positioning that controls progression of RNA polymerase through gene bodies. Our data further identifies Set2 as a key regulator of nucleosome positioning, involving the recruitment of chromatin remodelers to gene bodies.