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Hi. Make sure you save your paint layers before rendering or closing your file. You can do this automatically by clicking on the ZB object mode button (top right of screen in Zero Brush menu (far left of menu) next to sculpt mode icon and texture paint icon) to exit texture paint mode and return to object mode. Or you can manually press the “Save My Layers” button just below the option to change new layer sizes in the Zero Brush layer menu while in texture paint mode (right side of screen).