Greater than 85% of bases above Q30 at 2×50 bp
Greater than 80% of bases above Q30 at 2×100 bp
Greater than 80% of bases above Q30 at 2×125 bp

Greater than 85% of bases above Q30 at 2×50 bp
Greater than 80% of bases above Q30 at 2×100 bp

*Install specifications based on Illumina PhiX control library at supported cluster densities (between 610-678 K clusters/mm2 passing filter using TruSeq v3 Kits or 870-930 K clusters/mm2 passing filter using HiSeq v4). Run times for high output mode correspond to sequencing only. Performance may vary based on sample quality, cluster density, and other experimental factors.

Rapid Run Mode*

HISEQ Rapid SBS Kit v2 specifications

Read length

Dual Flow Cell

Single Flow Cell

Dual Flow Cell Run Time

1×36

18-22 Gb

9-11 Gb

7 hr

2×50

50-60 Gb

25-30 Gb

16 hr

2×100

100-120 Gb

50-60 Gb

27 hr

2×150

150-180 Gb

75-90 Gb

40 hr

2×250

250-300 Gb

125-150 Gb

60 hr

Reads Passing Filter (2 lanes per flow cell)

Up to 600 million single read or 1.2 billion paired-end reads

Up to 300 million single read or 600 million paired-end reads

Quality

Greater than 85% of bases above Q30 at 2×50 bp
Greater than 80% of bases above Q30 at 2×100 bp
Greater than 75% of bases above Q30 at 2×250 bp

*Install specifications based on Illumina PhiX control library at supported cluster densities (between 700-820 K clusters/mm2 passing filter using HiSeq Rapid v2 Kits). Run times for rapid run mode correspond to on-board cluster generation (1.5 hr) and sequencing. Performance may vary based on sample quality, cluster density, and other experimental factors. Early HiSeq 2000 instruments will run slightly slower when upgraded to a HiSeq 2500.

Innovative technologies
At Illumina, our goal is to apply innovative technologies to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. It is mission critical for us to deliver innovative, flexible, and scalable solutions to meet the needs of our customers. As a global company that places high value on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics.