Synthetic peptide corresponding to Human LAMP2B aa 350 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). LAMP2 has 3 distinct isoforms, LAMP2A, 2B & 2C which are expressed in different tissues and differ in sequence at the extreme C-terminus.Database link: P13473(Peptide available as ab23321)

ポジティブ･コントロール

This antibody gave a positive signal in the following whole cell lysates: HeLa, HEK293.
This antibody gave a positive signal in the following Methanol fixed cell lines: HeLa.
It also gave a positive signal in Human skin tissue sections.

製品の特性

製品の状態

Liquid

保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

アプリケーション

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション

Abreviews

特記事項

WB

Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).

ターゲット情報

機能

Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.

組織特異性

Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver.

関連疾患

Danon disease

配列類似性

Belongs to the LAMP family.

翻訳後修飾

O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The blot was incubated with ab18529 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

LAMP2B undergoes extensive glycosylation so ab18529 detects a band of 110 kDa by Western blot.