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What sets our kits apart?

Our kits use acetylcholinesterase (AChE) as the enzymatic label. AChE has
the fastest turnover rate of any enzymatic label. AChE assays are developed with Ellman's Reagent, which contains acetylthiocholine as the
substrate.
The final product of the enzymatic reaction, 5-thio-2-nitrobenzoic acid, is bright yellow and can be read at
405-420
nm.

AChE offers several advantages over other enzymes conventionally used in ELISAs:

Kinetic Superiority. Hydrolysis of acetylthiocholine by AChE shows true first-order
kinetics
with a turnover of 64,000 sec-1. Thus, AChE has a wider dynamic range and greater sensitivity
than
other labeling enzymes.

Low Background. Non-enzymatic hydrolysis of acetylthiocholine in buffer is essentially
absent.
Consequently, AChE has a low background and an increased signal/noise ratio as compared to TMB (a
peroxidase
substrate) which is inherently unstable.

High Sensitivity and Precision. AChE assays offer sensitivity that surpasses most other
immunoassays (both ELISA and RIA). Percent CVs average ≤10.

Versatility. AChE is a completely stable enzyme, unlike peroxidase which is suicidal.
Thus, if
an AChE plate is accidentally splashed or dropped during development, it can be re-developed by washing
the
plate and adding fresh Ellman's Reagent.

Shelf Life. Unlike Alkaline Phosphatase (AP) tracers, AChE can be lyophilized allowing
tracers
prepared with unstable molecules such as LTE4 or LTC4 to be stored for more than a
year
without loss of activity. For stable analytes, such as 8-isoprostane, AChE tracers are remarkably stable
in
solution for prolonged periods of time even at room temperature (see Figure below).