Dear netters,
long time no see...
been too busy IRL to surf the net.....have been webbing and mooing though!!!
Ok. what do i want....?
Is their anyone out their that can give me any tips on direct sequencing
of YACS. I have a large YAC (not purified away from yeast genomic DNA) and
would like to know if i could sequence this without subcloning.
We have use of an ABI 373 DNA sequencer and I am wondering if i could use
this to sequence my yac!
Any tips/comments on sequencing YACs would be gratefully received....
Feel free to email....although i will now check up on good 'ol methds_reagnts.
ciao...
BTW - getting married on new year's eve!!!! to a mad sicilian!!
Martin
--
..... Martin Leach Email:leach at mbcrrc.bu.edu
_|____ Dept. of Pharmacology Phone: (617) 638-5323
/ o / Boston Univ. School of Med. Fax: (617) 638-4329
_/ |-/__==/ 80 E. Concord St. (L603)
(BULLDOZER) \_ Boston MA 02118 "Not the old underpants on your
USA head.....WIBBLE" -BLACKADDER