Supplementary Material

The supplementary materials include the detail information for 25 aligners collected from different websites and publication (Supplementary file 1), the list of the aligners involved in the evaluation and comparison process (Supplementary file 2), numbers of citations for multiple aligners reflecting their popularity (Supplementary file 3), a python script used as memory usage monitor (Supplementary file 4) and a Perl script for computational simulation of in silico data (Supplementary file 5).