Determined by the dose-dependent stimulation of the proliferation of the human TF-1 cells. The expected ED50 for this effect is 20 - 40 ng/ml.

Species Reactivity

Human, Mouse

Synonyms

Kitlg; Mgf; SCF; Kitl

Description

Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. Recombinant rat SCF is an 18.4 kDa polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.