Observation

After the addition of 0.1M AgNO3 into 50mL LB medium, the solution immediately turned opaque. Due to the small amount of AgNO3 added into solutions containing AgNO3 with the following concentration: 1uM, 10uM, 100uM, 1mM, and due to the limit of LB medium present, no controls were set for these samples.

The solution containing cells and 10mM AgNO3 appeared to be the most opaque, a control that contained 45mL LB and 5mL 0.1M AgNO3 were also run at the same time.

The opaque color of solution containing cells 10mM AgNO3, as well as the control containing 45mL LB and 5mL of 0.1M AgNO3, turned darker over time, eventually, at the point of 15 hours, the color of solution turned darkish gray.

The opaque color might be due to silver ions in solution being oxidized by the chloride present in LB media, turning a darker color.