Change in the activity of the microsomal system of liver oxidation in rabbits in experimental atherosclerosis

Abstract: Oxidizing ability of rabbit liver tissue was studied in experimental atheromatosis, while the functional activity of microsomal enzymatic system was evaluated by content of cytochromes P-450 and b5 as well by estimation of the reductase activity and the rates of oxidation of aniline, dimethylalanine, p-nitroanisole and amidopyrine with NADN and NADPH as cosubstrates. In rabbits with atheromatous impairment of aorta all the patterns studied were decreased by 30-70%. Severity of the aorta impairment correlated directly with the liver tissue deterioration and the rate of alteration in activity of the microsomal system oxidation constituents. The most distinct changes were found in NADPH-dependent reactions and in cytochrome P-450. The data obtained suggest that experimental atheromatosis of rabbits, produced after cholesterol loading, is responsible for distinct impairment of liver microsomal enzymatic system. The most profound alterations were observed in the components involved in the cholesterol-7 alpha-hydroxylase reaction.