Data obtained from measuring the HbA1c content of serum samples using HRP labeled HCA179 antibody. Included were patient samples with abnormal hemoglobin (Hb). Besides binding to the normal glycated hemoglobin (Control 1, 2 and 3), HCA179 also recognizes the glycated hemoglobin variants HbC, HbE, HbD and to a lesser degree HbS. Elevated HbF cannot be detected due to the absence of the hemoglobin β chain. Values are given as % HbA1c. Samples were obtained from heterozygous donors. The values reflect the percentage of abnormal Hb carried by the donor. * Data from triplicate experiments

Data obtained from measuring the HbA1c content of serum samples using HRP labeled HCA179 antibody. Included were patient samples with abnormal hemoglobin (Hb). Besides binding to the normal glycated hemoglobin (Control 1, 2 and 3), HCA179 also recognizes the glycated hemoglobin variants HbC, HbE, HbD and to a lesser degree HbS. Elevated HbF cannot be detected due to the absence of the hemoglobin ß chain. Values are given as mmol/mol HbA1c.1. Samples were obtained from heterozygous donors. The values reflect the mmol/mol of abnormal Hb carried by the donor. The mmol/mol values were calculated using the IFCC standardization formula:NGSP = [0.09148 x IFCC] + 2.152* Data from triplicate experiments

Summary

Secondary Antibodies

Negative Isotype Controls

Useful Reagents

Positive Controls

Histology Controls

More Images

References

Reviews

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Human anti Human HbA1c antibody, clone 15783 recognizes HbA1c, i.e. hemoglobin when glycated at the N-terminal valine of the hemoglobin β chain. It does not bind to non-glycated hemoglobin. Besides binding to the normal glycated hemoglobin, it also recognizes the glycated hemoglobin variants HbC, HbE, HbD and to a lesser degree HbS. Elevated HbF cannot be detected due to the absence of the hemoglobin β chain.

The measurement of HbA1c levels is used to identify the average plasma glucose concentration. In Diabetes mellitus, higher levels of HbA1c have been associated with cardiovascular disease and other diseases. Levels of more than 6.5% HbA1c (48 mmol/mol) are used as a criterion to diagnose diabetes.

Intended Use

Target Species

Human

Product Form

A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.

Reconstitution

Preparation

StrepTactin affinity chromatography.

Preservative Stabilisers

0.01% Thiomersal

Immunogen

The immunogens used in the generation of this antibody were: A glycated peptide, corresponding to N-terminal 9 amino acids of haemoglobin beta chain coupled to carrier proteins.Purified HbA1c.

Purity

Affinity

The affinity of this antibody, as measured against the immunogen, was measured as KD=19 nM by real time, label-free molecular interaction analysis using HbA1c-BSA and HbA1c-TRF conjugates as immobilized antigen.

The affinity of this antibody, as measured against purified HbA1c, was measured as KD=58 nM by real time, label-free molecular interaction analysis using full length HbA1c as immobilized antigen.

Approx. Protein Concentrations

Total protein concentration 0.5 mg/ml.

Reagents In The Kit

Preparing The Antibody

Test Principle

Buffer Solution

Phosphate buffered saline.

Storage

Store at +4oC or at -20oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.

Licensed Use

For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.

Regulatory

For research purposes only

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name

Yes

No

Min Dilution

Max Dilution

ELISA

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.