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I made my first LC about 2 or more weeks ago usign jars similar to how agar does it with the jar and two tubes filled with polyfill.The LC was a 3-4% Kara mix.

After bout 2-3days i notcied the first signs of growth and it was lookin like it was doing ok for the first week but since then i havent noticed hardly any more growth. The temps havent changed in the TIT and the jars in there with them are colinising just as fast.I couldnt seem to locate a post where it had the total colinsation time for a LC so dunno if its going slow or not.

Perhaps it is runnin out of O2, maybe polyfill packed in too tight, anyone recommend i remove it and inject some air in thier with a shyringe, i just dun wanna help increase any chances of contams. It wouldnt of run out of sugar to feed on would it?

Cheers

--------------------Old Ben

I beleive that my job in mappin the impulse pathways of a single neuron can allow us to open a mecca into the quailitative functions of the human geno...

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