DyLight Fluors Technology and Product Guide

Choose an appropriate fluorescent labeling reagent or probe.

Thermo Scientific DyLight Fluorescent Dyes are a complete family of high-intensity, photostable fluorescent tags for labeling antibodies and other molecular probes.

The DyLight Fluors are available as reactive labeling agents and as conjugates of secondary antibodies, biotin-binding proteins and molecular weight markers for use in fluorescence microscopy, flow cytometry, ELISA, high-content screening and other array platforms.

DyLight Fluors have absorption maxima ranging from 350nm to 777nm, covering the entire visible light spectrum and several key near-infared and infrared wavelengths. The absorption and emission properties of the DyLight Fluors match the output (excitation) and detection wavelengths of common fluorescence instrumentation.

The DyLight Dyes exhibit higher fluorescence intensity and photostability than Alexa Fluor*, CyDye* and LI-COR* Dyes in many applications and remain highly fluorescent over a broad pH range (pH 4-9). Additionally, the water solubility of the DyLight Dyes allows a high dye-to-protein ratio to be achieved without causing precipitation of conjugates.

|Emission spectra of Thermo Scientific DyLight Fluors.| See table below for additional specifications and links to detailed information pages about each dye.

Spectral Properties of DyLight Fluorescent Dyes. Each dye name and spectrum-image links to a dedicated information page that includes the detailed spectra, example data and literature references.

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The DyLight Fluorescent Dyes are available in both NHS ester-activated and maleimide-activated forms, which provide for efficient and specific labeling of primary amines and sulfhydryl groups, respectively. Several DyLight Fluors are also available with azide-reactive phosphine groups for detection and labeling using Staudinger ligation chemistry. Certain DyLight Fluors are also available as free acids for use as non-reactive negative controls.

The amine-reactive DyLight Fluors are available as both stand-alone reagents and complete kits optimized for milligram- and microgram-scale antibody or general protein labeling reactions. The DyLight Fluor Antibody Labeling Kits include the amine-reactive DyLight Fluors, buffers and special columns for easy clean-up of the labeling reaction and recovery of the labeled protein. The Dye Removal Columns are also available separately.

Highly Cross-Adsorbed Secondary Antibody Conjugates – these forms of secondary antibodies are specially purified to minimize binding to off-target immunoglobulins, making them ideal for multiplex immunofluorescence assays where cross-reactivity is a concern.

High Content Screening Assays – Thermo Scientific High Content Reagent-Based Assays are multiplex fluorescent assays for cellular imaging and analysis. Each one of these many assays uses DyLight Fluor Antibodies and other fluorescent probes to detect the cellular expression and translocation of specific proteins and cell structural parameters. When used in combination with appropriate imaging equipment and analysis software, these assays provide extremely powerful strategies for examining cellular responses.