This research had as an objective to evaluate genotype of corn to see the resistance to Colletotrichum graminicola, in field conditions and greenhouse conditions, through artificial inoculations with isolated pathogens obtained in locations where the disease is prevelant. The research was setup in two locations: Fazenda Experimental da FCAV/Unesp Câmpus Jaboticabal - SP in December 2004 and Embrapa Milho e Sorgo/CNPMS Sete Lagoas - MG in January 2005 and may 2005 in the greenhouse of CNPMS. According to the data obtained in the experiment, there is evidence of the existence to races of Colletotrichum graminicola among the isolates. In the plots of Jaboticabal, the lowest values of AUDPC were observed in the genotypes BRS 3003 (triple hybrid), followed by BRS 2020 (double hydrid). In Sete Lagoas, the lowest values of AUDPC were from the hybrids BRS 3003 and CMS 101142 (simple hybrid). In general the hybrid BRS 3003 showed the lowest values of AUDPC, suggesting that the greater genetic variability (intercrossing of three genotypes) of this material was better protection against the pathogen.