Progesterone ELISA Kit(55R-RE52231)

Overview

This Progesterone ELISA Kit is a ELISA based on the principle of competitive binding. The microtiter wells are coated with a polyclonal antibody directed towards an antigenic site on the Progesterone molecule. Endogenous Progesterone of a patient sample competes with a Progesterone horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is reverse proportional to the concentration of Progesterone in the sample. After addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of Progesterone in the patient sample.

Usage & Assay Information

Usage Recommendations

Optimal conditions to be determined by end user

Storage & Safety

Storage

Store at 4 deg C.

Biohazard Information

All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV, HbsAg and HCV. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as pote

General Information

Biological Significance

Progesterone (pregn-4-ene-3, 20-dione) is a C21 steroid hormone containing a keto-group (at C-3) and a double bond between C-4 and C-5 ( Delta4). This steroid hormone is a female sex hormone which, in conjunction with estrogens, regulates the accessory organs during the menstrual cycle and it is particularly important in preparing the endometrium for the implantation of the blastocyte and in maintaining pregnancy.