Request a quote for a bulk order

for Recombinant PRMT5 proteinContents

for Recombinant PRMT5 proteinBackground

PRMT5 (Protein Arginine Methyltransferase 5, also known as JBP1, Janus Binding Protein 1) is a type II arginine methyltransferase, a protein that transfers methyl groups to the arginine residues of substrate proteins. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT5 symmetrically dimethylates histone H2A Arg3, H3 Arg8 and H4 Arg3. It also methylates Piwi proteins which regulate small non-coding RNAs and snRNPs, regulating snRNP core particle assembly. PRMT5 functions as a co-repressor of transcription and is recruited by a number of DNA binding proteins and transcriptional repressor complexes. It is present both in the nucleus and the cytoplasm. It also interacts with COPR5, JAK2, PRDM1 and RPS10.

for Recombinant PRMT5 proteinApplication Notes

Recombinant PRMT5 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. PRMT5 is an active H3R8 and H4R3 methyltransferase.

for Recombinant PRMT5 proteinProtein Details

Recombinant PRMT5 (accession number NP_006100.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 74.2 kDa. The recombinant protein is >70% pure by SDS-PAGE.

Recombinant PRMT5 protein gel.PRMT5 protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.