Podoplanin (gp36) is a 36kD type I cell membrane-associated glycoprotein, with a broad spectrum of distribution, being strongly expressed on the lymphatic endothelium found in lung, placenta and skeletal muscle and to a lesser extent in brain, kidney and liver. Podoplanin is absent on blood endothelium and therefore this antibody is able to distinguish between lymphatic vessels and blood vasculature.

Catalog #

P9104-91C

Applications

Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution

Immunofluorescence (IC): 1-2ug

Western Blot: 1:100-1:2000

Immunoprecipitation: 1:10-1:500

Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Clone Type

Polyclonal

Isotype

IgG

Host

Rabbit

Source

Human

Concentration

~1mg/ml

Form

Supplied as a liquid in PBS, pH 7.4.

Purity

Purified by Protein A affinity chromatography.

Immunogen

Recombinant ectodomain of human gp36 (podoplanin).

Specificity

Recognizes human Podoplanin.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.