In this paper, the separation distance between two neighboring offshore wind turbines has been carried out by using the Actuator Line/Navier-Stokes technique developed at the Technical University of Denmark (DTU). Under offshore atmospheric conditions, Large Eddy Simulation has been performed for two Tjæreborg 2 MW wind turbines in tandem with separation distances of 4D, 5D, 6D, 7D, 8D and 10D at the design wind speed of 10 m/s. The power performance of the wake turbine showed to be about 23% of the first turbine at a separation distance of 4D while its performance reached about 50% at 7D due to the turbulence mixing. This study hints that the optimal separation distance between neighboring turbines for offshore wind farms should be 7 rotor diameters.