Recombinant Pasteurella multocida toxin

This product is derived from a recombinant ToxA with His6-tag at N-termina expressed in E. coli. Its molecular weight is 145 kDa.

Description:

Pasteurella multocida toxin (PMT) is produced by a gram-negative bacillus, Pasteurella multocida. PMT activates the Gq and G12/13 dependent signaling pathways. Gq and G12/13 are alpha subunits of the GTP trimer bound protein of animal cells. It does not activate the highly related G11-dependent pathways. This toxin binds to a ganglioside-type cell surface receptor, acts intracellularly after having been internalized through an endocytic pathway, and has pleiotropic effects on cell physiology. Therefore it does not function on receptor-deficient cells or cells defective in the endocytosis pathway. PMT is encoded by the toxA gene of P. multocida.

Source:

E. coli

Species:

Pasteurella multocida

Tag:

His

Form:

50% glycerol, 5mM Tris-HCl(pH7.5), 0.1M NaCl

Purity:

More than 90% purity by SDS-PAGE (CBB staining)

Applications:

1) For the studies on the GTP trimer bound protein- dependent signaling pathway 2) As a PMT antigen for immunological assays such as ELISA, western blotting and dot blotting.