Eyebots cannot be "activated" (spoken to or pickpocketed), but they can be killed or hacked via the Robotics Expertperk. They are one of the weakest enemies in the game and can generally be killed with a single hit from most weapons. Unlike the eyebots encountered in previous Fallout titles, eyebots in the Commonwealth do not explode upon death.

An eyebot which is distinguishable by its appearance. This eyebot serves the Mechanist and uses a laser gun as a weapon to attack the Sole Survivor, although the eyebot has pretty low health on lower difficulties, and can become elusive due to it randomly moving to another location very quickly to attack you.

During the final fight of Restoring Order, some variations of the eyebot may appear with the name of "Trashbot." These eyebots are visually identical to the servomech and cybermech variants, but are significantly weaker.

With the Automatron DLC installed, the player can have access to their own eyebots which can find materials (such as adhesive) for their settlements. However, you cannot build your own eyebot with the robot workbench.

Even though many eyebots can be seen in the DLC, the eyebot cannot be customized in any way.