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Publication identifier and Species Publication identifier PMID Must be entered into queue and checked out prior to annotation and closed after annotation Ensures traceability, prevents redundancy. Species NCBI Taxonomy id

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Outcome and User info Outcome Positive, neutral or negative Was the sequence proven functional? Yes, no, or uncertain Sequences can only be considered negative or positive for the conditions under which they were tested User information Encourages ownership and accountability Associated with every record, comment, and validation a user creates Three roles: ‘User’, ‘Validator’, ‘Administrator’

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Discussion items Discussion item: Should a record reference only one publication? What should be done in cases where several papers describe experimental validation of the same regulatory sequence? Discussion item: Should further sub-categorization of regulatory regions be allowed (e.g. Silencer, enhancer, locus-control region, etc) Discussion item: In a case where sequence conservation is perfect between the species of interest and model organism (e.g. both mouse and human have identical regulatory sequence upstream of an orthologous gene) could an assay in one be considered evidence for function of the sequence in both species? Discussion item: Should multiple TFs be allowed for a single record? Or should this form a second record? Discussion item: Should TF complexes be allowed? Discussion item: What is the minimal evidence we should allow? Discussion item: Are there other evidence classes that should be included? Discussion item: Should ORegAnno migrate to a more complex or formal ontology system for evidence.