Eukaryotic cells must replicate DNA within the confines of chromatin. The basic unit of chromatin is the nucleosome, which consists of approximately 147 base pairs of DNA wrapped around an octamer of histone proteins. ...

<italic>Helicobacter pylori</italic> chronically colonizes the stomachs of approximately 50% of the world's population, with outcomes ranging from gastritis to ulcers and gastric cancer. <italic>H. pylori</italic> is ...

University of Washington Abstract Genetic Mapping and Functional Characterization of Zebrafish Mutants That are Hypersusceptible to Mycobacterium marinum Russell David Berg Chair of the Supervisory Committee: Lalita ...

Bone marrow failure and myelodysplastic syndromes (BMF/MDS) are disorders of impaired blood cell production with increased leukemia risk. BMF/MDS may be acquired or inherited, a distinction critical for treatment selection. ...

Transcription from genomic DNA is regulated in many ways and governs cellular identity, growth, and homeostasis. If transcription becomes deregulated, it can serve a critical role in the development of cancer and other ...

Accurate DNA replication and RNA transcription are critically important for proper cell functioning: the fidelity of these processes is crucial; infidelity can lead to cellular dysfunction and disease. The key problem in ...

Duplication of a cell's genome is a central requirement for the propagation of life. This duplication, a highly regulated process known as DNA replication, occurs in S-phase and is initiated at genomic sites termed origins ...

Novel functionally altered transcripts may be recurrent in prostate cancer (PCa) and may underlie lethal and advanced disease and the neuroendocrine small cell carcinoma (SCC) phenotype. We conducted an RNASeq survey of ...

Glioblastoma multiforme (GBM) is the most common and aggressive type of brain cancer and is notoriously drug and radiation resistant. Current therapies and trials have repeatedly failed to substantially increase the survival ...

Glioblastoma Multiforme (GBM) is the most common and aggressive form of brain cancer. ~90% of adult GBM patients receiving standard of care therapies die within 2 years of diagnosis due to ineffective therapies. To ...

It has been known for decades that cigarette smoking is associated with atherosclerosis; however, the specific mechanisms for this association remain unclear. High density lipoprotein (HDL) is known to play a key ...

Barrett's esophagus is a human premalignant condition in which the normal squamous epithelial lining of the esophagus is replaced by a metaplastic columnar epithelium. Patients with Barrett's esophagus are at increased ...

Targeted genome engineering is a technology that has the potential to vastly improve clinical medicine, as well as biological research. With the ability to design enzymes targeting any given DNA locus of interest, and ...

Double-stranded RNA (dsRNA) that accumulates during many viral infections is recognized by the host cell and elicits an antiviral response. Protein kinase R (PKR) is activated by dsRNA binding, phosphorylates eIF2α, and ...

Phenotypic heterogeneity is a commonly observed phenomenon in cell populations that can provide a selective advantage within diverse, unpredictable conditions. Second messenger-based signaling, having a global impact on ...

As organisms cycle between feeding and fasting, they must balance nutritional input with energy expenditures such as reproduction, growth, and repair. To achieve the proper equilibrium, these processes must be tightly ...

Clathrin-mediated endocytosis (CME) is an essential process by which cells internalize plasma membrane proteins. The master regulator of the process is thought to be the adaptor protein AP2, which organizes receptors, ...

MicroRNAs are 21-22 nucleotide RNAs that regulate gene expression by binding mRNA 3' UTRs and inhibiting translation or targeting them for degradation. The Xpcl1 locus, encoding the miR-106a~363 miRNA cluster, was first ...

Rhabdomyosarcoma (RMS) is a pediatric tumor of skeletal muscle that fails to undergo terminal differentiation, even though it expresses the myogenic regulatory factor MyoD, which should be sufficient for that process. We ...