Klíčová slova:
Festulolium, Genome composition, Genomic in situ hybridization, Grass hybrids, Homoeologous recombination, Lolium × FestucaAbstrakt:
Festulolium are hybrids between fescue ( Festuca ) and ryegrass
( Lolium ) species and combine high seed yield of ryegrasses
with abiotic stress tolerance of fescues. Chromosomes
of Festuca and Lolium present in Festulolium freely
pair and recombine, which results in highly variable progeny
where every single plant has a unique chromosome constitution.
Thus, the stability of the genomic composition in Festulolium
cultivars is an important issue. In this work, we used
in situ hybridization to examine the genomic composition
(understood as the proportion of parental genomes present)
over 3 consecutive generations of propagation via outcrossing
(the first one being the generation used for cultivar registration)
of 3 Festulolium cultivars. Our analysis revealed that
the genome composition largely differs among the plants
from individual cultivars but appears to be relatively stable
over the generations. A gradual shift in the genome composition
towards Lolium observed in the early generations of
hybrids appears to reach a plateau where the proportions of
parental genomes become stabilized. Nevertheless, the pro-
portion remains unbalanced to a certain extent (always in
favor of the Lolium genome) in each cultivar. Our observations
indicate a possibility to modulate genomic composition
in hybrids by breeders’ selection without a compromise
on stability.DOI:10.1159/000458746Fulltext: kontaktujte autory z ÚEBAutoři z ÚEB:Jaroslav Doležel, David Kopecky