The aminoglycoside residues are extracted by centrifugation from bovine liver, kidney and muscle tissue (free from fat and connective tissue) using a buffer containing ammonium acetate/trichloroacetic acid (NH4OAc,EDTA,NaCl,TCA buffer). Tobramycin is used
as an internal standard. The sample extracts (adjusted to pH 6.50 plus minus 0.05) are cleaned-up with a weak-cation exchange sorbent using disposable pipette extraction followed by elution with ten percent formic acid in water. The final extract is analyzed
using ion-pair reverse phase UHPLC/MS/MS in positive electrospray ionization mode. A C18 waters UPLC BEH column is used for separation.

Applicable Concentration Range

Amikacin, apramycin, dihydrostreptomycin, gentamicin, hygromycin B, kanamycin, neomycin B, spectinomycin may be detected in bovine kidney, muscle, and liver at various levels as detailed in the attached SOP.