Protocols

Specificity / Sensitivity

Bax Antibody (Human-Specific) detects endogenous levels of total Bax protein. Cross-reativity was not detected with other family members at physiological conditions.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding lysine 58 of human Bax. Antibodies were purified by protein A and peptide affinity chromatography.

IHC-P (paraffin)

Background

Bax is a key component for cellular induced apoptosis through mitochondrial stress (1). Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane (2). Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis (3,4).