Rumors of homosexuality

On The Little Rascals cartoon series, in the 30-second skit "Out on a Limb", Alfalfa was seen pushing Spanky on a swing hanging from a tree limb, which could be perceived as being homosexual - until Darla appeared and invited Alfalfa on a date.

Human-to-animal attractions

On Hong Kong Phooey, Rosemary (human) is infatuated with the eponymous martial artist hero (dog), but she couldn't care less about his alter ego, Penrod Pooch.

Animal-to-human attractions

A running gag on Jabberjaw is the eponymous shark smooching Shelly, to her disgust.

Kissing

In three known episodes of The Little Rascals - "Rascals' Revenge", "Rock & Roll Rascals" and "Horse Sense" - Darla was seen kissing Alfalfa on the cheek at some point. In Porky-O and Julie-Et, Julie Jamm was seen kissing Porky on the cheek near the end of the segment, and in The Zero Hero, midway through the episode, Maria kissed Alfalfa after saving her from injury.

Human nudity and lewd humor

Male nudity

In The Jetsons syndicated episode "Invisibly Yours, George", George becomes invisible while testing one of Mr. Spacely's inventions, but George's clothing is still visible. George's invisibility wears off while he's stark naked, but his genitalia are either hidden or outside camera range.

In one short on Dexter's Laboratory, Dexter's mother sees him nude on the front sidewalk, with his hands over his crotch. She is concerned only with the absence of his glasses.

In one Johnny Bravo short, Johnny leaves his bathtub with only a newspaper to cover him.

Underwear exposure

In the "Carnival Calamity" episode of The Perils of Penelope Pitstop, Penelope grabs a loose thread on one of the Hooded Claw's pant cuffs. While it slows her descent, it also exposes his boxer shorts.

In the Pac-Man episode "Journey to the Center of Pacland", after Pac-Man's allies chomp Mezmaron's cloak to shreds, the villain's boxer shorts are exposed as he returns to his Lava Lair.

In the Dexter's Laboratory short "The Big Sister", the giant Dee Dee strips Dexter to his briefs as if he were a doll.

In the Richie Rich segment "The Day the Estate Stood Still", Professor Keenbean was seen in his underwear while hanging on a mechanical hand on his clothes changing machine.

Also, in the Riches segment "Wiped Out!", Reggie Van Dough was seen in his underwear after he got busted for selling the Rich family's assets for a cheap price.

Plus, in the Riches segment "Caveboy Richie", Richie is seen in his underwear after moths from one of Keenbean's older inventions have eaten up both Richie's and Keenbean's clothing.

In The Scooby and Scrappy-Doo/Puppy Hour segment "Comic Book Caper", Shaggy and Scooby captured the culprit while disguised as a horse, revealing the culprit's underwear at a comic book convention.

Taxes

At the end of the Huckleberry Hound short "The Unmasked Avenger", Huck promises to improve the peasants' way of life: better roads, free education and the like. His popularity wanes suddenly when he points out that all the improvements will require taxes.

Death

In the Smurfs episode "Squeaky", in the wake of a fire, Papa Smurf informed Smurfette that the mouse she adopted had passed away. This scene was edited out when the episode was part of the syndicated rerun package.

The beginning of the Pac-Man episode "Pacula" takes place in a cemetery.

A 1983 episode of The Little Rascals, titled "Fright Night", involves Alfalfa and Spanky in a cemetery in search of ghosts.