How do you know if your protein is well-behaved in certain buffers or if it aggregates over time in others? Is your protein still a monomer after thawing or reconstitution after freeze drying? Are your buffer conditions favourable to protein crystallization? The Zetasizer APS allows you to investigate the stability of your protein over a wide range of developmental conditions with minimal user intervention.

Our primary use of the Zetasizer APS is in screening protein preparations to ensure they are monodisperse and therefore suitable for crystallization. If we find aggregated proteins, then crystallization trials are unlikely to be successful and we know we need to adjust the preparation process. We’ve been running the Zetasizer APS continuously since 2012 and the real beauty is that we simply load the samples in multiwell plates, leave it to run, even overnight, and go back and pick up the results. The system is easy to use, requires little training and, most importantly, the measurements and data are excellent.