Hsp20 is a member of the family of small heat shock proteins (sHsps), with homology to Hsp25, Hsp27, and alphaB-crystallin. Highest expression of Hsp20 is found in skeletal, smooth, and cardiac muscle. Under heat shock, Hsp20 translocates from the cytosol to the nucleus, although the amount of Hsp20 in the cell does not increase like other Hsps. Hsp20 oligomerization and interaction with polymeric actin filaments is regulated via phosphorylation by PKA and PKG

Catalog #

H1834

Applications

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution

Western Blot (Colormetric): 1:1000 detects a band at ~20kD

Optimal dilutions to be determined by the researcher.

Positive Controls

HeLa Cell Lysate

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Clone Type

Polyclonal

Isotype

IgG

Host

Rabbit

Source

Rat

Concentration

~1mg/ml

Form

Supplied as a liquid in PBS, pH 7.0, 3% BSA, 0.09% sodium azide.

Purity

Pruified by peptide affinity chromatography.

Immunogen

Rat skeletal muscle Hsp20

Specificity

Recognizes rat Heat Shock Protein 20.

Species crossreactivity

Human, mouse, bovine, canine, fish

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.