Figure 2.

Double-color probe set for the detection of the hTERC gain and immunohistological
detection for hTERT in cervical dysplasia.A. interphase FISH shows a normal signal pattern containing two copies each of the
hTERC ( red) and CEP3 (green) (X630). B. interphase FISH shows increased number of signals for hTERC (red) compared to CEP3(green)
(X630) C. interphase FISH shows gain of both hTERC (red) and CEP3 (green) gains in the larger
cells (X630). D. Weakly hTERT positive cells are found in the suprabasal cell layers. (IHC for hTERT,
original magnification× 400) E. A high-grade CIN3 lesion, where hTERT positive cells
are found throughout the full thickness of the epithelium. (IHC for hTERT, original
magnification×400) F. An invasive squamous cell carcinoma with strong hTERT expression. The staining intensity
varies from weak to intense dark brown, indicating that the expression levels of hTERT
vary from cell to cell. (IHC for hTERT, original magnification× 400).