In 2001, she co-founded an art collective-turned-fashion line, Imitation of Christ, which featured pieces hand-sewn solely from recycled vintage and thrift store clothing, and has since worked primarily as a conceptual artist. In 2015, she made her debut as a director with the horror film #Horror (2015), which was picked up for distribution by IFC Midnight.

In 2017, Subkoff joined the group of women making the Harvey Weinstein sexual abuse allegations, saying that the producer had sexually harassed her in the 1990s when she applied for a role in one of his films, and then had her blacklisted when she resisted. "It became impossible for me to get work as an actress after this, so I then had to start a new career path and started Imitation of Christ", she said.[8]

Reflecting on the line, Subkoff said: “We were talking about waste, throwing things away, and taking something that’s old and making it new again, putting the human hand back into a world that reeks of manufacturing. It felt very appropriate to do that in 2000."[9]

After Subkoff and Damhave parted ways, she continued to design pieces for the line through 2006. In 2007, Subkoff sold the label to Josh Sparks, the former chief executive of the Australian label Sass & Bide, for a reported $2 million.[12] The following year, in 2008, the label went out of business. In 2012, Subkoff revived the label and began working on more pieces, shortening the brand name to simply "Imitation."[12]

In 2012, Subkoff created a ten-day art installation and continuous performance piece at the Carlton Festival of the Arts in São Paulo, Brazil.[9] She also conceptualized a performance piece in a group show curated by Dimitri Antonitsis in Hydra, Greece.

She exhibited a three hour long installation at the Bortolami Gallery in New York City during the 2012 New York Fashion Week titled "This is Not a Fashion Show," which featured a girl's choir in leotards performing “Carol of the Bells” (intimated as a "slight to Yuletide consumerism") and "performers aging from 8 to 70 pruned and posing in front of antique mirrors lining the gallery walls."[9] In explaining the idea behind the show, Subkoff said: "We are a society that only sells commodities. We do not create anything unless it's to be bought and sold, so the idea of doing something where there isn't a commodity to sell, or what the commodity is to sell is very confusing, is extremely interesting to me."[14]

In 2013, she collaborated with Milla Jovovich on a filmed installation in Venice, Italy titled "Future/Perfect," which had Jovovich enclosed in a glass house, surrounded by boxes with consumer logos, artwork, and clothing.[15]

Though working predominantly in art, Subkoff also had a minor appearance in the film, including Tanner Hall (2009) and Abandoned (2010), with Brittany Murphy. Subkoff made her directorial debut in 2014 with the horror film#Horror (2015), which details a group of wealthy adolescent girls who experience a night of violence and terror after a social media game is tinged with cyberbullying. The film was screened out of competition at the 2015 Cannes Film Festival, and was picked up for distribution by IFC Midnight, slated for a limited release in November 2015. According to Subkoff, she conceived the film after a conversation she'd had with her friend's daughter: "[The idea] started because I asked my friend's daughter, "What is horror, to you?" This girl was cyberbullied very badly... Now, I was bullied badly as a kid, but I could always change schools. I could always go home. Now you can't…when bullying follows you home, and there's no escape and no end, to me, that's horror. And to so many girls, that's just life."[16]

Subkoff has been formerly romantically linked to Wes Anderson, and was formerly engaged to director Tom Hooper.[2] In the fall of 2014, Subkoff married artist Urs Fischer.[17][18][19] Subkoff gave birth to a daughter, Grace, in May 2016.[20] In June 2016, it was reported that Subkoff and Fischer had filed for divorce.[18] Subkoff resides in Los Angeles, California.[21]

^"United States Public Records, 1970-2009," database, FamilySearch (22 May 2014), Tara Lyn Subkoff, Residence, West Hollywood, California, United States; a third party aggregator of publicly available information.