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class=\u0022elements-frag-data highwire-markup\u0022 id=\u0022fig-data\u0022\u003E\u003Cdiv id=\u0022fig-data-figures\u0022 class=\u0022group frag-figures\u0022\u003E\u003Cdiv class=\u0022fig-data-title-jump clearfix\u0022\u003E\u003Ch3 id=\u0022fig-frag-data-title\u0022 class=\u0022fig-data-group-title\u0022\u003EFigures\u003C\/h3\u003E\u003Cdiv class=\u0022fig-data-jump-links\u0022\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cdiv class=\u0022item-list\u0022\u003E\u003Cul id=\u0022fig-frag-fig\u0022 class=\u0022fig-frag-data-list clearfix\u0022\u003E\u003Cli class=\u0022first\u0022\u003E\u003Cdiv class=\u0022element-fig-frag-data clearfix supplementary-material-caption\u0022\u003E\u003Cdiv class=\u0022highwire-markup\u0022\u003E\u003Cdiv xmlns=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022 id=\u0022content-block-markup\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cdiv class=\u0022fig-expansion\u0022 id=\u0022F1\u0022\u003E\u003Cspan class=\u0022highwire-journal-article-marker-start\u0022\u003E\u003C\/span\u003E\u003Cdiv class=\u0022highwire-figure\u0022\u003E\u003Cdiv class=\u0022fig-inline-img-wrapper\u0022\u003E\u003Cdiv class=\u0022fig-inline-img\u0022\u003E\u003Ca href=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F1.large.jpg?width=800\u0026amp;height=600\u0026amp;carousel=1\u0022 title=\u0022Generation and verification of Z4 mutations. The genomic region with the two alternatively spliced exons of the gene CG12974 (red bars) and the transcript of Z4 depicting its translated regions (blue bars) and untranslated regions (yellow bars) is shown. Arrows indicate the direction of transcription. The site of the EP-element (green triangle) insertion in line EP(3)0756 is shown, from which deletions were generated by imprecise excision to generate the homozygous lethal lines Z4-1.3 and Z4-7.1. The extent of the deletions is indicated by the broken lines. In line Z4-1.3 the deletion encompasses 800 bp of DNA including the 5\u0026#x2032;-transcription start of Z4 and the 5\u0026#x2032;-region of the gene CG12974 encoding two exons of the transcript CG12974-RA. In line Z4-7.1 1800 bp to the left of the EP-element are deleted, which removes part of the transcription units of gene CG12974, but leaves the coding region of Z4 unaffected. The bar below indicates the genomic region that complements the lethal mutation of lines Z4-1.3 and Z4-7.1. Within this bar, the vertical line shows the position of the myc-tag that was fused in frame to the 3\u0026#x2032;-end of the Z4 coding region. Chromosomes from larvae transgenic for the tagged genomic region were stained with DAPI (A) and a monoclonal anti-myc antibody (B). The composite image (C) reveals the localization of the tagged Z4 protein to the chromosomal interbands.\u0022 class=\u0022highwire-fragment fragment-images colorbox-load\u0022 rel=\u0022gallery-fragment-images-1140701508\u0022 data-figure-caption=\u0022\u0026lt;div class=\u0026quot;highwire-markup\u0026quot;\u0026gt;Generation and verification of Z4 mutations. The genomic region with the two alternatively spliced exons of the gene CG12974 (red bars) and the transcript of Z4 depicting its translated regions (blue bars) and untranslated regions (yellow bars) is shown. Arrows indicate the direction of transcription. The site of the EP-element (green triangle) insertion in line EP(3)0756 is shown, from which deletions were generated by imprecise excision to generate the homozygous lethal lines Z4-1.3 and Z4-7.1. The extent of the deletions is indicated by the broken lines. In line Z4-1.3 the deletion encompasses 800 bp of DNA including the 5\u0026#x2032;-transcription start of Z4 and the 5\u0026#x2032;-region of the gene CG12974 encoding two exons of the transcript CG12974-RA. In line Z4-7.1 1800 bp to the left of the EP-element are deleted, which removes part of the transcription units of gene CG12974, but leaves the coding region of Z4 unaffected. The bar below indicates the genomic region that complements the lethal mutation of lines Z4-1.3 and Z4-7.1. Within this bar, the vertical line shows the position of the myc-tag that was fused in frame to the 3\u0026#x2032;-end of the Z4 coding region. Chromosomes from larvae transgenic for the tagged genomic region were stained with DAPI (A) and a monoclonal anti-myc antibody (B). The composite image (C) reveals the localization of the tagged Z4 protein to the chromosomal interbands.\u0026lt;\/div\u0026gt;\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003E\u003Cspan class=\u0022hw-responsive-img\u0022\u003E\u003Cimg class=\u0022highwire-fragment fragment-image lazyload\u0022 alt=\u0022 Fig. 2. \u0022 src=\u0022data:image\/gif;base64,R0lGODlhAQABAIAAAAAAAP\/\/\/yH5BAEAAAAALAAAAAABAAEAAAIBRAA7\u0022 data-src=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F1.medium.gif\u0022\/\u003E\u003Cnoscript\u003E\u003Cimg class=\u0022highwire-fragment fragment-image\u0022 alt=\u0022 Fig. 2. \u0022 src=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F1.medium.gif\u0022\/\u003E\u003C\/noscript\u003E\u003C\/span\u003E\u003C\/a\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cul class=\u0022highwire-figure-links inline\u0022\u003E\u003Cli class=\u0022download-fig first\u0022\u003E\u003Ca href=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F1.large.jpg?download=true\u0022 class=\u0022highwire-figure-link highwire-figure-link-download\u0022 title=\u0022Download Fig. 2. \u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload figure\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022new-tab\u0022\u003E\u003Ca href=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F1.large.jpg\u0022 class=\u0022highwire-figure-link highwire-figure-link-newtab\u0022 target=\u0022_blank\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EOpen in new tab\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022download-ppt last\u0022\u003E\u003Ca href=\u0022\/highwire\/powerpoint\/1561165\u0022 class=\u0022highwire-figure-link highwire-figure-link-ppt\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload powerpoint\u003C\/a\u003E\u003C\/li\u003E\u003C\/ul\u003E\u003C\/div\u003E\u003Cdiv class=\u0022fig-caption\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cspan class=\u0022fig-label\u0022\u003E\n \u003Cstrong\u003EFig. 2.\u003C\/strong\u003E\n \u003C\/span\u003E \n \u003Cp id=\u0022p-13\u0022\u003EGeneration and verification of \u003Cem\u003EZ4\u003C\/em\u003E mutations. The genomic region with the two alternatively spliced exons of the gene \u003Cem\u003ECG12974\u003C\/em\u003E (red bars) and the transcript of \u003Cem\u003EZ4\u003C\/em\u003E depicting its translated regions (blue bars) and untranslated regions (yellow bars) is shown. Arrows indicate the direction of transcription. The site of the EP-element (green triangle) insertion in line EP(3)0756 is shown, from which deletions were generated by imprecise excision to generate the homozygous lethal lines \u003Cem\u003EZ4-1.3\u003C\/em\u003E and \u003Cem\u003EZ4-7.1\u003C\/em\u003E. The extent of the deletions is indicated by the broken lines. In line \u003Cem\u003EZ4-1.3\u003C\/em\u003E the deletion encompasses 800 bp of DNA including the 5\u2032-transcription start of \u003Cem\u003EZ4\u003C\/em\u003E and the 5\u2032-region of the gene \u003Cem\u003ECG12974\u003C\/em\u003E encoding two exons of the transcript CG12974-RA. In line \u003Cem\u003EZ4-7.1\u003C\/em\u003E 1800 bp to the left of the EP-element are deleted, which removes part of the transcription units of gene \u003Cem\u003ECG12974\u003C\/em\u003E, but leaves the coding region of \u003Cem\u003EZ4\u003C\/em\u003E unaffected. The bar below indicates the genomic region that complements the lethal mutation of lines \u003Cem\u003EZ4-1.3\u003C\/em\u003E and \u003Cem\u003EZ4-7.1\u003C\/em\u003E. Within this bar, the vertical line shows the position of the myc-tag that was fused in frame to the 3\u2032-end of the \u003Cem\u003EZ4\u003C\/em\u003E coding region. Chromosomes from larvae transgenic for the tagged genomic region were stained with DAPI (A) and a monoclonal anti-myc antibody (B). The composite image (C) reveals the localization of the tagged Z4 protein to the chromosomal interbands.\u003C\/p\u003E\n \u003Cdiv class=\u0022sb-div caption-clear\u0022\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cspan class=\u0022highwire-journal-article-marker-end\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003Cspan id=\u0022related-urls\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/li\u003E\u003Cli\u003E\u003Cdiv class=\u0022element-fig-frag-data clearfix supplementary-material-caption\u0022\u003E\u003Cdiv class=\u0022highwire-markup\u0022\u003E\u003Cdiv xmlns=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022 id=\u0022content-block-markup\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cdiv class=\u0022fig-expansion\u0022 id=\u0022F2\u0022\u003E\u003Cspan class=\u0022highwire-journal-article-marker-start\u0022\u003E\u003C\/span\u003E\u003Cdiv class=\u0022highwire-figure\u0022\u003E\u003Cdiv class=\u0022fig-inline-img-wrapper\u0022\u003E\u003Cdiv class=\u0022fig-inline-img\u0022\u003E\u003Ca href=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F2.large.jpg?width=800\u0026amp;height=600\u0026amp;carousel=1\u0022 title=\u0022Z4 expression and localization to chromosomal interbands. (A) Polytene chromosomes of wild-type 3rd instar larvae were double labeled with DAPI (blue) and the Z4 antibody (red). Z4 stains the euchromatic arms of the chromosomes (a) and is absent from the heterochromatic chromocenter (b). Within euchromatin, Z4 stains exclusively the interbands (c). In heat-shocked wild-type larvae Z4 demarcates the distal edge of the 87A hsp70 heat-shock puff (d, marked by the white arrowhead) and the proximal edge of the hsp70 heat-shock puff in 87C (yellow arrowhead). (B) Early embryos from wild-type flies (a-d) and Kc cells (e,f) were double labeled with DAPI (a,c,e) and the Z4 antibody (b,d,f). In the early embryo Z4 is ubiquitously expressed. The staining of Kc cells shows that Z4 is chromosomally associated during interphase (e,f). By contrast, Z4 does not associate with mitotic chromosomes of early embryos but disperses within the cell (c,d). (C) Western blot of nuclear proteins from wild-type embryos (E) and Kc cells (Kc) with the Z4 antibody. In addition to antigens that are uniquely present in Kc cells or embryos, the antibody recognizes the 170 kDa Z4 protein, which is present in both fractions.\u0022 class=\u0022highwire-fragment fragment-images colorbox-load\u0022 rel=\u0022gallery-fragment-images-1140701508\u0022 data-figure-caption=\u0022\u0026lt;div class=\u0026quot;highwire-markup\u0026quot;\u0026gt;Z4 expression and localization to chromosomal interbands. (A) Polytene chromosomes of wild-type 3rd instar larvae were double labeled with DAPI (blue) and the Z4 antibody (red). Z4 stains the euchromatic arms of the chromosomes (a) and is absent from the heterochromatic chromocenter (b). Within euchromatin, Z4 stains exclusively the interbands (c). In heat-shocked wild-type larvae Z4 demarcates the distal edge of the 87A hsp70 heat-shock puff (d, marked by the white arrowhead) and the proximal edge of the hsp70 heat-shock puff in 87C (yellow arrowhead). (B) Early embryos from wild-type flies (a-d) and Kc cells (e,f) were double labeled with DAPI (a,c,e) and the Z4 antibody (b,d,f). In the early embryo Z4 is ubiquitously expressed. The staining of Kc cells shows that Z4 is chromosomally associated during interphase (e,f). By contrast, Z4 does not associate with mitotic chromosomes of early embryos but disperses within the cell (c,d). (C) Western blot of nuclear proteins from wild-type embryos (E) and Kc cells (Kc) with the Z4 antibody. In addition to antigens that are uniquely present in Kc cells or embryos, the antibody recognizes the 170 kDa Z4 protein, which is present in both fractions.\u0026lt;\/div\u0026gt;\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003E\u003Cspan class=\u0022hw-responsive-img\u0022\u003E\u003Cimg class=\u0022highwire-fragment fragment-image lazyload\u0022 alt=\u0022 Fig. 1. \u0022 src=\u0022data:image\/gif;base64,R0lGODlhAQABAIAAAAAAAP\/\/\/yH5BAEAAAAALAAAAAABAAEAAAIBRAA7\u0022 data-src=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F2.medium.gif\u0022\/\u003E\u003Cnoscript\u003E\u003Cimg class=\u0022highwire-fragment fragment-image\u0022 alt=\u0022 Fig. 1. \u0022 src=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F2.medium.gif\u0022\/\u003E\u003C\/noscript\u003E\u003C\/span\u003E\u003C\/a\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cul class=\u0022highwire-figure-links inline\u0022\u003E\u003Cli class=\u0022download-fig first\u0022\u003E\u003Ca href=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F2.large.jpg?download=true\u0022 class=\u0022highwire-figure-link highwire-figure-link-download\u0022 title=\u0022Download Fig. 1. \u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload figure\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022new-tab\u0022\u003E\u003Ca href=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F2.large.jpg\u0022 class=\u0022highwire-figure-link highwire-figure-link-newtab\u0022 target=\u0022_blank\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EOpen in new tab\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022download-ppt last\u0022\u003E\u003Ca href=\u0022\/highwire\/powerpoint\/1561172\u0022 class=\u0022highwire-figure-link highwire-figure-link-ppt\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload powerpoint\u003C\/a\u003E\u003C\/li\u003E\u003C\/ul\u003E\u003C\/div\u003E\u003Cdiv class=\u0022fig-caption\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cspan class=\u0022fig-label\u0022\u003E\n \u003Cstrong\u003EFig. 1.\u003C\/strong\u003E\n \u003C\/span\u003E \n \u003Cp id=\u0022p-32\u0022\u003EZ4 expression and localization to chromosomal interbands. (A) Polytene chromosomes of wild-type 3rd instar larvae were double labeled with DAPI (blue) and the Z4 antibody (red). Z4 stains the euchromatic arms of the chromosomes (a) and is absent from the heterochromatic chromocenter (b). Within euchromatin, Z4 stains exclusively the interbands (c). In heat-shocked wild-type larvae Z4 demarcates the distal edge of the 87A \u003Cem\u003Ehsp70\u003C\/em\u003E heat-shock puff (d, marked by the white arrowhead) and the proximal edge of the \u003Cem\u003Ehsp70\u003C\/em\u003E heat-shock puff in 87C (yellow arrowhead). (B) Early embryos from wild-type flies (a-d) and Kc cells (e,f) were double labeled with DAPI (a,c,e) and the Z4 antibody (b,d,f). In the early embryo Z4 is ubiquitously expressed. The staining of Kc cells shows that Z4 is chromosomally associated during interphase (e,f). By contrast, Z4 does not associate with mitotic chromosomes of early embryos but disperses within the cell (c,d). (C) Western blot of nuclear proteins from wild-type embryos (E) and Kc cells (Kc) with the Z4 antibody. In addition to antigens that are uniquely present in Kc cells or embryos, the antibody recognizes the 170 kDa Z4 protein, which is present in both fractions.\u003C\/p\u003E\n \u003Cdiv class=\u0022sb-div caption-clear\u0022\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cspan class=\u0022highwire-journal-article-marker-end\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003Cspan id=\u0022related-urls\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/li\u003E\u003Cli\u003E\u003Cdiv class=\u0022element-fig-frag-data clearfix supplementary-material-caption\u0022\u003E\u003Cdiv class=\u0022highwire-markup\u0022\u003E\u003Cdiv xmlns=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022 id=\u0022content-block-markup\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cdiv class=\u0022fig-expansion\u0022 id=\u0022F3\u0022\u003E\u003Cspan class=\u0022highwire-journal-article-marker-start\u0022\u003E\u003C\/span\u003E\u003Cdiv class=\u0022highwire-figure\u0022\u003E\u003Cdiv class=\u0022fig-inline-img-wrapper\u0022\u003E\u003Cdiv class=\u0022fig-inline-img\u0022\u003E\u003Ca href=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F3.large.jpg?width=800\u0026amp;height=600\u0026amp;carousel=1\u0022 title=\u0022Dose-dependent effect of Z4 on wm4 position-effect variegation. (A) Representative eye phenotypes of males derived from a cross between homozygous wm4 females and males heterozygous for the Z4 mutants balanced over TM3, Sb. Compared with the males with the TM3 chromosomes, the eyes of sibling males mutant for Z4 have increased numbers of red ommatidia. (B) Quantitative measurement of eye pigments of the phenotypes shown in (A). Eye pigments were extracted from the heads of 20 representative individual males for each cross and their absorbance at 480 nm was determined. The columns show the ratios of absorbances between the Z4 mutants and their TM3 siblings derived from the identical cross. The control shows the ratios obtained from wm4\/Y;e,st,spo\/+ and wm4\/Y;TM3,Sb\/+ siblings. (C) Eye phenotypes of males from a cross between wm4\/wm4 females and w1118\/Y;P[Z4myc]\/CyO males. Males with three copies of the Z4 gene have significantly reduced numbers of red ommatidia compared with their sibling males with two doses of Z4.\u0022 class=\u0022highwire-fragment fragment-images colorbox-load\u0022 rel=\u0022gallery-fragment-images-1140701508\u0022 data-figure-caption=\u0022\u0026lt;div class=\u0026quot;highwire-markup\u0026quot;\u0026gt;Dose-dependent effect of Z4 on wm4 position-effect variegation. (A) Representative eye phenotypes of males derived from a cross between homozygous wm4 females and males heterozygous for the Z4 mutants balanced over TM3, Sb. Compared with the males with the TM3 chromosomes, the eyes of sibling males mutant for Z4 have increased numbers of red ommatidia. (B) Quantitative measurement of eye pigments of the phenotypes shown in (A). Eye pigments were extracted from the heads of 20 representative individual males for each cross and their absorbance at 480 nm was determined. The columns show the ratios of absorbances between the Z4 mutants and their TM3 siblings derived from the identical cross. The control shows the ratios obtained from wm4\/Y;e,st,spo\/+ and wm4\/Y;TM3,Sb\/+ siblings. (C) Eye phenotypes of males from a cross between wm4\/wm4 females and w1118\/Y;P[Z4myc]\/CyO males. Males with three copies of the Z4 gene have significantly reduced numbers of red ommatidia compared with their sibling males with two doses of Z4.\u0026lt;\/div\u0026gt;\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003E\u003Cspan class=\u0022hw-responsive-img\u0022\u003E\u003Cimg class=\u0022highwire-fragment fragment-image lazyload\u0022 alt=\u0022 Fig. 3. \u0022 src=\u0022data:image\/gif;base64,R0lGODlhAQABAIAAAAAAAP\/\/\/yH5BAEAAAAALAAAAAABAAEAAAIBRAA7\u0022 data-src=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F3.medium.gif\u0022\/\u003E\u003Cnoscript\u003E\u003Cimg class=\u0022highwire-fragment fragment-image\u0022 alt=\u0022 Fig. 3. \u0022 src=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F3.medium.gif\u0022\/\u003E\u003C\/noscript\u003E\u003C\/span\u003E\u003C\/a\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cul class=\u0022highwire-figure-links inline\u0022\u003E\u003Cli class=\u0022download-fig first\u0022\u003E\u003Ca href=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F3.large.jpg?download=true\u0022 class=\u0022highwire-figure-link highwire-figure-link-download\u0022 title=\u0022Download Fig. 3. \u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload figure\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022new-tab\u0022\u003E\u003Ca href=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F3.large.jpg\u0022 class=\u0022highwire-figure-link highwire-figure-link-newtab\u0022 target=\u0022_blank\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EOpen in new tab\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022download-ppt last\u0022\u003E\u003Ca href=\u0022\/highwire\/powerpoint\/1561178\u0022 class=\u0022highwire-figure-link highwire-figure-link-ppt\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload powerpoint\u003C\/a\u003E\u003C\/li\u003E\u003C\/ul\u003E\u003C\/div\u003E\u003Cdiv class=\u0022fig-caption\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cspan class=\u0022fig-label\u0022\u003E\n \u003Cstrong\u003EFig. 3.\u003C\/strong\u003E\n \u003C\/span\u003E \n \u003Cp id=\u0022p-39\u0022\u003EDose-dependent effect of \u003Cem\u003EZ4\u003C\/em\u003E on \u003Cem\u003Ewm4\u003C\/em\u003E position-effect variegation. (A) Representative eye phenotypes of males derived from a cross between homozygous \u003Cem\u003Ewm4\u003C\/em\u003E females and males heterozygous for the \u003Cem\u003EZ4\u003C\/em\u003E mutants balanced over \u003Cem\u003ETM3, Sb\u003C\/em\u003E. Compared with the males with the \u003Cem\u003ETM3\u003C\/em\u003E chromosomes, the eyes of sibling males mutant for \u003Cem\u003EZ4\u003C\/em\u003E have increased numbers of red ommatidia. (B) Quantitative measurement of eye pigments of the phenotypes shown in (A). Eye pigments were extracted from the heads of 20 representative individual males for each cross and their absorbance at 480 nm was determined. The columns show the ratios of absorbances between the \u003Cem\u003EZ4\u003C\/em\u003E mutants and their \u003Cem\u003ETM3\u003C\/em\u003E siblings derived from the identical cross. The control shows the ratios obtained from \u003Cem\u003Ew\u003Csup\u003Em4\u003C\/sup\u003E\/Y;e,st,spo\/+\u003C\/em\u003E and \u003Cem\u003Ew\u003Csup\u003Em4\u003C\/sup\u003E\/Y;TM3,Sb\/+\u003C\/em\u003E siblings. (C) Eye phenotypes of males from a cross between \u003Cem\u003Ew\u003Csup\u003Em4\u003C\/sup\u003E\/w\u003Csup\u003Em4\u003C\/sup\u003E\u003C\/em\u003E females and \u003Cem\u003Ew\u003Csup\u003E1118\u003C\/sup\u003E\/Y;P[Z4myc]\/CyO\u003C\/em\u003E males. Males with three copies of the \u003Cem\u003EZ4\u003C\/em\u003E gene have significantly reduced numbers of red ommatidia compared with their sibling males with two doses of \u003Cem\u003EZ4\u003C\/em\u003E.\u003C\/p\u003E\n \u003Cdiv class=\u0022sb-div caption-clear\u0022\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cspan class=\u0022highwire-journal-article-marker-end\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003Cspan id=\u0022related-urls\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/li\u003E\u003Cli\u003E\u003Cdiv class=\u0022element-fig-frag-data clearfix supplementary-material-caption\u0022\u003E\u003Cdiv class=\u0022highwire-markup\u0022\u003E\u003Cdiv xmlns=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022 id=\u0022content-block-markup\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cdiv class=\u0022fig-expansion\u0022 id=\u0022F4\u0022\u003E\u003Cspan class=\u0022highwire-journal-article-marker-start\u0022\u003E\u003C\/span\u003E\u003Cdiv class=\u0022highwire-figure\u0022\u003E\u003Cdiv class=\u0022fig-inline-img-wrapper\u0022\u003E\u003Cdiv class=\u0022fig-inline-img\u0022\u003E\u003Ca href=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F4.large.jpg?width=800\u0026amp;height=600\u0026amp;carousel=1\u0022 title=\u0022Morphology of chromosomes mutant for Z4. Tb+ homozygous mutant 3rd instar larvae were collected from the Z4-7.1\/TM6,Tb stock and chromosomal squashes were stained with DAPI (A-C). Whole mount salivary glands from wild-type (D,G) and Z4 mutants transheterozygous for the alleles Z4-1.3\/Z4-7.1 (E,H) were double stained with Sytox Green (D,E) and the Z4 antibody (G,H). Unfixed salivary glands dissected from larvae expressing the fusion construct BJ1-GFP (F) or from Z4-1.3\/Z4-7.1 mutant larvae transgenic for BJ1-GFP (I) were analyzed by CLSM. In Z4 mutants a pertubation of chromosomes with the appearance of decondensed chromatin is evident (C,E,I).\u0022 class=\u0022highwire-fragment fragment-images colorbox-load\u0022 rel=\u0022gallery-fragment-images-1140701508\u0022 data-figure-caption=\u0022\u0026lt;div class=\u0026quot;highwire-markup\u0026quot;\u0026gt;Morphology of chromosomes mutant for Z4. Tb+ homozygous mutant 3rd instar larvae were collected from the Z4-7.1\/TM6,Tb stock and chromosomal squashes were stained with DAPI (A-C). Whole mount salivary glands from wild-type (D,G) and Z4 mutants transheterozygous for the alleles Z4-1.3\/Z4-7.1 (E,H) were double stained with Sytox Green (D,E) and the Z4 antibody (G,H). Unfixed salivary glands dissected from larvae expressing the fusion construct BJ1-GFP (F) or from Z4-1.3\/Z4-7.1 mutant larvae transgenic for BJ1-GFP (I) were analyzed by CLSM. In Z4 mutants a pertubation of chromosomes with the appearance of decondensed chromatin is evident (C,E,I).\u0026lt;\/div\u0026gt;\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003E\u003Cspan class=\u0022hw-responsive-img\u0022\u003E\u003Cimg class=\u0022highwire-fragment fragment-image lazyload\u0022 alt=\u0022 Fig. 4. \u0022 src=\u0022data:image\/gif;base64,R0lGODlhAQABAIAAAAAAAP\/\/\/yH5BAEAAAAALAAAAAABAAEAAAIBRAA7\u0022 data-src=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F4.medium.gif\u0022\/\u003E\u003Cnoscript\u003E\u003Cimg class=\u0022highwire-fragment fragment-image\u0022 alt=\u0022 Fig. 4. \u0022 src=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F4.medium.gif\u0022\/\u003E\u003C\/noscript\u003E\u003C\/span\u003E\u003C\/a\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cul class=\u0022highwire-figure-links inline\u0022\u003E\u003Cli class=\u0022download-fig first\u0022\u003E\u003Ca href=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F4.large.jpg?download=true\u0022 class=\u0022highwire-figure-link highwire-figure-link-download\u0022 title=\u0022Download Fig. 4. \u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload figure\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022new-tab\u0022\u003E\u003Ca href=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F4.large.jpg\u0022 class=\u0022highwire-figure-link highwire-figure-link-newtab\u0022 target=\u0022_blank\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EOpen in new tab\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022download-ppt last\u0022\u003E\u003Ca href=\u0022\/highwire\/powerpoint\/1561184\u0022 class=\u0022highwire-figure-link highwire-figure-link-ppt\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload powerpoint\u003C\/a\u003E\u003C\/li\u003E\u003C\/ul\u003E\u003C\/div\u003E\u003Cdiv class=\u0022fig-caption\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cspan class=\u0022fig-label\u0022\u003E\n \u003Cstrong\u003EFig. 4.\u003C\/strong\u003E\n \u003C\/span\u003E \n \u003Cp id=\u0022p-42\u0022\u003EMorphology of chromosomes mutant for \u003Cem\u003EZ4. Tb\u003C\/em\u003E\u003Csup\u003E+\u003C\/sup\u003E homozygous mutant 3rd instar larvae were collected from the \u003Cem\u003EZ4-7.1\/TM6,Tb\u003C\/em\u003E stock and chromosomal squashes were stained with DAPI (A-C). Whole mount salivary glands from wild-type (D,G) and \u003Cem\u003EZ4\u003C\/em\u003E mutants transheterozygous for the alleles \u003Cem\u003EZ4-1.3\/Z4-7.1\u003C\/em\u003E (E,H) were double stained with Sytox Green (D,E) and the Z4 antibody (G,H). Unfixed salivary glands dissected from larvae expressing the fusion construct BJ1-GFP (F) or from \u003Cem\u003EZ4-1.3\/Z4-7.1\u003C\/em\u003E mutant larvae transgenic for BJ1-GFP (I) were analyzed by CLSM. In \u003Cem\u003EZ4\u003C\/em\u003E mutants a pertubation of chromosomes with the appearance of decondensed chromatin is evident (C,E,I).\u003C\/p\u003E\n \u003Cdiv class=\u0022sb-div caption-clear\u0022\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cspan class=\u0022highwire-journal-article-marker-end\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003Cspan id=\u0022related-urls\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/li\u003E\u003Cli\u003E\u003Cdiv class=\u0022element-fig-frag-data clearfix supplementary-material-caption\u0022\u003E\u003Cdiv class=\u0022highwire-markup\u0022\u003E\u003Cdiv xmlns=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022 id=\u0022content-block-markup\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cdiv class=\u0022fig-expansion\u0022 id=\u0022F5\u0022\u003E\u003Cspan class=\u0022highwire-journal-article-marker-start\u0022\u003E\u003C\/span\u003E\u003Cdiv class=\u0022highwire-figure\u0022\u003E\u003Cdiv class=\u0022fig-inline-img-wrapper\u0022\u003E\u003Cdiv class=\u0022fig-inline-img\u0022\u003E\u003Ca href=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F5.large.jpg?width=800\u0026amp;height=600\u0026amp;carousel=1\u0022 title=\u0022Presence of Z4 in chromosomes of Z4 hypomorphic mutants. (A) Chromosomes were squashed from 3rd instar larvae homozygous mutant for Z4-7.1. The DNA of the squashed chromosomes was stained with Sytox Green (green) and with the Z4 antibody (red). The composite image shows the localization of Z4 to some telomeres in addition to a few internal chromosomal sites, which are still complementary to the DNA staining. (B,C) Chromosomes from homozygous mutant larvae of the line Z4-1.3 that were rescued by the expression of the Z4 cDNA were squashed and stained with DAPI (blue) and the Z4 antibody (red). Compared with its staining of the interbands, Z4 is concentrated on the telomere of the X chromosome (B) and on the telomere of the left arm of chromosome 2 (C).\u0022 class=\u0022highwire-fragment fragment-images colorbox-load\u0022 rel=\u0022gallery-fragment-images-1140701508\u0022 data-figure-caption=\u0022\u0026lt;div class=\u0026quot;highwire-markup\u0026quot;\u0026gt;Presence of Z4 in chromosomes of Z4 hypomorphic mutants. (A) Chromosomes were squashed from 3rd instar larvae homozygous mutant for Z4-7.1. The DNA of the squashed chromosomes was stained with Sytox Green (green) and with the Z4 antibody (red). The composite image shows the localization of Z4 to some telomeres in addition to a few internal chromosomal sites, which are still complementary to the DNA staining. (B,C) Chromosomes from homozygous mutant larvae of the line Z4-1.3 that were rescued by the expression of the Z4 cDNA were squashed and stained with DAPI (blue) and the Z4 antibody (red). Compared with its staining of the interbands, Z4 is concentrated on the telomere of the X chromosome (B) and on the telomere of the left arm of chromosome 2 (C).\u0026lt;\/div\u0026gt;\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003E\u003Cspan class=\u0022hw-responsive-img\u0022\u003E\u003Cimg class=\u0022highwire-fragment fragment-image lazyload\u0022 alt=\u0022 Fig. 5. \u0022 src=\u0022data:image\/gif;base64,R0lGODlhAQABAIAAAAAAAP\/\/\/yH5BAEAAAAALAAAAAABAAEAAAIBRAA7\u0022 data-src=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F5.medium.gif\u0022\/\u003E\u003Cnoscript\u003E\u003Cimg class=\u0022highwire-fragment fragment-image\u0022 alt=\u0022 Fig. 5. \u0022 src=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F5.medium.gif\u0022\/\u003E\u003C\/noscript\u003E\u003C\/span\u003E\u003C\/a\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cul class=\u0022highwire-figure-links inline\u0022\u003E\u003Cli class=\u0022download-fig first\u0022\u003E\u003Ca href=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F5.large.jpg?download=true\u0022 class=\u0022highwire-figure-link highwire-figure-link-download\u0022 title=\u0022Download Fig. 5. \u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload figure\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022new-tab\u0022\u003E\u003Ca href=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F5.large.jpg\u0022 class=\u0022highwire-figure-link highwire-figure-link-newtab\u0022 target=\u0022_blank\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EOpen in new tab\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022download-ppt last\u0022\u003E\u003Ca href=\u0022\/highwire\/powerpoint\/1561191\u0022 class=\u0022highwire-figure-link highwire-figure-link-ppt\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload powerpoint\u003C\/a\u003E\u003C\/li\u003E\u003C\/ul\u003E\u003C\/div\u003E\u003Cdiv class=\u0022fig-caption\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cspan class=\u0022fig-label\u0022\u003E\n \u003Cstrong\u003EFig. 5.\u003C\/strong\u003E\n \u003C\/span\u003E \n \u003Cp id=\u0022p-45\u0022\u003EPresence of Z4 in chromosomes of \u003Cem\u003EZ4\u003C\/em\u003E hypomorphic mutants. (A) Chromosomes were squashed from 3rd instar larvae homozygous mutant for \u003Cem\u003EZ4-7.1\u003C\/em\u003E. The DNA of the squashed chromosomes was stained with Sytox Green (green) and with the Z4 antibody (red). The composite image shows the localization of Z4 to some telomeres in addition to a few internal chromosomal sites, which are still complementary to the DNA staining. (B,C) Chromosomes from homozygous mutant larvae of the line \u003Cem\u003EZ4-1.3\u003C\/em\u003E that were rescued by the expression of the \u003Cem\u003EZ4\u003C\/em\u003E cDNA were squashed and stained with DAPI (blue) and the Z4 antibody (red). Compared with its staining of the interbands, Z4 is concentrated on the telomere of the X chromosome (B) and on the telomere of the left arm of chromosome 2 (C).\u003C\/p\u003E\n \u003Cdiv class=\u0022sb-div caption-clear\u0022\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cspan class=\u0022highwire-journal-article-marker-end\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003Cspan id=\u0022related-urls\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/li\u003E\u003Cli\u003E\u003Cdiv class=\u0022element-fig-frag-data clearfix supplementary-material-caption\u0022\u003E\u003Cdiv class=\u0022highwire-markup\u0022\u003E\u003Cdiv xmlns=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022 id=\u0022content-block-markup\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cdiv class=\u0022fig-expansion\u0022 id=\u0022F6\u0022\u003E\u003Cspan class=\u0022highwire-journal-article-marker-start\u0022\u003E\u003C\/span\u003E\u003Cdiv class=\u0022highwire-figure\u0022\u003E\u003Cdiv class=\u0022fig-inline-img-wrapper\u0022\u003E\u003Cdiv class=\u0022fig-inline-img\u0022\u003E\u003Ca href=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F6.large.jpg?width=800\u0026amp;height=600\u0026amp;carousel=1\u0022 title=\u0022Binding of Z4 to Notch sequences in vitro. (A) 5\u0026#x2032;-region of Notch. The black bar represents the transcribed region of Notch, and the gray bar indicates the region deleted in the faswb-allele. The position of the N1 and N2 fragments that were used for in vitro binding to Z4 are shown below. Both fragments were digested and endlabeled at the HinfI-sites indicated. (B) Electrophoretic mobility shift assay with 0.5 \u0026#x3BC;g of purified Z4 (omitted in lanes 1 and 8), 5 ng of the end-labeled DNA fragments N1 (lanes 1-7) or N2 (lanes 8-14) and a 100-, 200-, 400- or 800-fold excess of poly(dI-dC) competitor DNA (lanes 3-6 and 10-13) as indicated on the top.\u0022 class=\u0022highwire-fragment fragment-images colorbox-load\u0022 rel=\u0022gallery-fragment-images-1140701508\u0022 data-figure-caption=\u0022\u0026lt;div class=\u0026quot;highwire-markup\u0026quot;\u0026gt;Binding of Z4 to Notch sequences in vitro. (A) 5\u0026#x2032;-region of Notch. The black bar represents the transcribed region of Notch, and the gray bar indicates the region deleted in the faswb-allele. The position of the N1 and N2 fragments that were used for in vitro binding to Z4 are shown below. Both fragments were digested and endlabeled at the HinfI-sites indicated. (B) Electrophoretic mobility shift assay with 0.5 \u0026#x3BC;g of purified Z4 (omitted in lanes 1 and 8), 5 ng of the end-labeled DNA fragments N1 (lanes 1-7) or N2 (lanes 8-14) and a 100-, 200-, 400- or 800-fold excess of poly(dI-dC) competitor DNA (lanes 3-6 and 10-13) as indicated on the top.\u0026lt;\/div\u0026gt;\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003E\u003Cspan class=\u0022hw-responsive-img\u0022\u003E\u003Cimg class=\u0022highwire-fragment fragment-image lazyload\u0022 alt=\u0022 Fig. 6. \u0022 src=\u0022data:image\/gif;base64,R0lGODlhAQABAIAAAAAAAP\/\/\/yH5BAEAAAAALAAAAAABAAEAAAIBRAA7\u0022 data-src=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F6.medium.gif\u0022\/\u003E\u003Cnoscript\u003E\u003Cimg class=\u0022highwire-fragment fragment-image\u0022 alt=\u0022 Fig. 6. \u0022 src=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F6.medium.gif\u0022\/\u003E\u003C\/noscript\u003E\u003C\/span\u003E\u003C\/a\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cul class=\u0022highwire-figure-links inline\u0022\u003E\u003Cli class=\u0022download-fig first\u0022\u003E\u003Ca href=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F6.large.jpg?download=true\u0022 class=\u0022highwire-figure-link highwire-figure-link-download\u0022 title=\u0022Download Fig. 6. \u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload figure\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022new-tab\u0022\u003E\u003Ca href=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F6.large.jpg\u0022 class=\u0022highwire-figure-link highwire-figure-link-newtab\u0022 target=\u0022_blank\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EOpen in new tab\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022download-ppt last\u0022\u003E\u003Ca href=\u0022\/highwire\/powerpoint\/1561196\u0022 class=\u0022highwire-figure-link highwire-figure-link-ppt\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload powerpoint\u003C\/a\u003E\u003C\/li\u003E\u003C\/ul\u003E\u003C\/div\u003E\u003Cdiv class=\u0022fig-caption\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cspan class=\u0022fig-label\u0022\u003E\n \u003Cstrong\u003EFig. 6.\u003C\/strong\u003E\n \u003C\/span\u003E \n \u003Cp id=\u0022p-49\u0022\u003EBinding of Z4 to \u003Cem\u003ENotch\u003C\/em\u003E sequences in vitro. (A) 5\u2032-region of Notch. The black bar represents the transcribed region of \u003Cem\u003ENotch\u003C\/em\u003E, and the gray bar indicates the region deleted in the \u003Cem\u003Efa\u003Csup\u003Eswb\u003C\/sup\u003E\u003C\/em\u003E-allele. The position of the N1 and N2 fragments that were used for in vitro binding to Z4 are shown below. Both fragments were digested and endlabeled at the \u003Cem\u003EHin\u003C\/em\u003EfI-sites indicated. (B) Electrophoretic mobility shift assay with 0.5 \u03bcg of purified Z4 (omitted in lanes 1 and 8), 5 ng of the end-labeled DNA fragments N1 (lanes 1-7) or N2 (lanes 8-14) and a 100-, 200-, 400- or 800-fold excess of poly(dI-dC) competitor DNA (lanes 3-6 and 10-13) as indicated on the top.\u003C\/p\u003E\n \u003Cdiv class=\u0022sb-div caption-clear\u0022\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cspan class=\u0022highwire-journal-article-marker-end\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003Cspan id=\u0022related-urls\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/li\u003E\u003Cli class=\u0022last\u0022\u003E\u003Cdiv class=\u0022element-fig-frag-data clearfix supplementary-material-caption\u0022\u003E\u003Cdiv class=\u0022highwire-markup\u0022\u003E\u003Cdiv xmlns=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022 id=\u0022content-block-markup\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cdiv class=\u0022fig-expansion\u0022 id=\u0022F7\u0022\u003E\u003Cspan class=\u0022highwire-journal-article-marker-start\u0022\u003E\u003C\/span\u003E\u003Cdiv class=\u0022highwire-figure\u0022\u003E\u003Cdiv class=\u0022fig-inline-img-wrapper\u0022\u003E\u003Cdiv class=\u0022fig-inline-img\u0022\u003E\u003Ca href=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F7.large.jpg?width=800\u0026amp;height=600\u0026amp;carousel=1\u0022 title=\u0022Identification of a chromodomain protein interacting with Z4. (A) Proteins were immunoprecipitated from nuclear extracts prepared from Kc cells with the anti-Z4 antibody, resolved by SDS-PAGE on a 8% gel and visualized by Coomassie staining. The major band at a relative molecular weight of 150-160 kDa was eluted from the gel and analyzed by MALDI mass spectrometry. The band labeled Chriz is the protein encoded by the gene CG10712. (B-D) Polytene chromosomes of a transgenic line that expressed a myc-tagged Chriz protein in salivary glands were stained with an antibody against the myc-tag. The DNA staining (blue) is complementary to the staining of myc-Chriz (red), showing its interband localization on all chromosomes (A). myc-Chriz is restricted to the euchromatic parts of the chromosomes and does not bind to the heterochromatic chromocentre (C). At higher resolution the localization of myc-Chriz to a high number of interbands is obvious from the optical section shown in (D). It is identical to the interband localization of Z4.\u0022 class=\u0022highwire-fragment fragment-images colorbox-load\u0022 rel=\u0022gallery-fragment-images-1140701508\u0022 data-figure-caption=\u0022\u0026lt;div class=\u0026quot;highwire-markup\u0026quot;\u0026gt;Identification of a chromodomain protein interacting with Z4. (A) Proteins were immunoprecipitated from nuclear extracts prepared from Kc cells with the anti-Z4 antibody, resolved by SDS-PAGE on a 8% gel and visualized by Coomassie staining. The major band at a relative molecular weight of 150-160 kDa was eluted from the gel and analyzed by MALDI mass spectrometry. The band labeled Chriz is the protein encoded by the gene CG10712. (B-D) Polytene chromosomes of a transgenic line that expressed a myc-tagged Chriz protein in salivary glands were stained with an antibody against the myc-tag. The DNA staining (blue) is complementary to the staining of myc-Chriz (red), showing its interband localization on all chromosomes (A). myc-Chriz is restricted to the euchromatic parts of the chromosomes and does not bind to the heterochromatic chromocentre (C). At higher resolution the localization of myc-Chriz to a high number of interbands is obvious from the optical section shown in (D). It is identical to the interband localization of Z4.\u0026lt;\/div\u0026gt;\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003E\u003Cspan class=\u0022hw-responsive-img\u0022\u003E\u003Cimg class=\u0022highwire-fragment fragment-image lazyload\u0022 alt=\u0022 Fig. 7. \u0022 src=\u0022data:image\/gif;base64,R0lGODlhAQABAIAAAAAAAP\/\/\/yH5BAEAAAAALAAAAAABAAEAAAIBRAA7\u0022 data-src=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F7.medium.gif\u0022\/\u003E\u003Cnoscript\u003E\u003Cimg class=\u0022highwire-fragment fragment-image\u0022 alt=\u0022 Fig. 7. \u0022 src=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F7.medium.gif\u0022\/\u003E\u003C\/noscript\u003E\u003C\/span\u003E\u003C\/a\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cul class=\u0022highwire-figure-links inline\u0022\u003E\u003Cli class=\u0022download-fig first\u0022\u003E\u003Ca href=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F7.large.jpg?download=true\u0022 class=\u0022highwire-figure-link highwire-figure-link-download\u0022 title=\u0022Download Fig. 7. \u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload figure\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022new-tab\u0022\u003E\u003Ca href=\u0022http:\/\/jcs.biologists.org\/content\/joces\/117\/18\/4253\/F7.large.jpg\u0022 class=\u0022highwire-figure-link highwire-figure-link-newtab\u0022 target=\u0022_blank\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EOpen in new tab\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022download-ppt last\u0022\u003E\u003Ca href=\u0022\/highwire\/powerpoint\/1561202\u0022 class=\u0022highwire-figure-link highwire-figure-link-ppt\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload powerpoint\u003C\/a\u003E\u003C\/li\u003E\u003C\/ul\u003E\u003C\/div\u003E\u003Cdiv class=\u0022fig-caption\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cspan class=\u0022fig-label\u0022\u003E\n \u003Cstrong\u003EFig. 7.\u003C\/strong\u003E\n \u003C\/span\u003E \n \u003Cp id=\u0022p-52\u0022\u003EIdentification of a chromodomain protein interacting with \u003Cem\u003EZ4\u003C\/em\u003E. (A) Proteins were immunoprecipitated from nuclear extracts prepared from Kc cells with the anti-\u003Cem\u003EZ4\u003C\/em\u003E antibody, resolved by SDS-PAGE on a 8% gel and visualized by Coomassie staining. The major band at a relative molecular weight of 150-160 kDa was eluted from the gel and analyzed by MALDI mass spectrometry. The band labeled Chriz is the protein encoded by the gene \u003Cem\u003ECG10712\u003C\/em\u003E. (B-D) Polytene chromosomes of a transgenic line that expressed a myc-tagged Chriz protein in salivary glands were stained with an antibody against the myc-tag. The DNA staining (blue) is complementary to the staining of myc-Chriz (red), showing its interband localization on all chromosomes (A). myc-Chriz is restricted to the euchromatic parts of the chromosomes and does not bind to the heterochromatic chromocentre (C). At higher resolution the localization of myc-Chriz to a high number of interbands is obvious from the optical section shown in (D). It is identical to the interband localization of Z4.\u003C\/p\u003E\n \u003Cdiv class=\u0022sb-div caption-clear\u0022\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cspan class=\u0022highwire-journal-article-marker-end\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003Cspan id=\u0022related-urls\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/li\u003E\u003C\/ul\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E \u003C\/div\u003E\n\n \n \u003C\/div\u003E\n\u003Cdiv class=\u0022panel-separator\u0022\u003E\u003C\/div\u003E\u003Cdiv class=\u0022panel-pane pane-earthchem\u0022 \u003E\n \n \n \n \u003Cdiv class=\u0022pane-content\u0022\u003E\n \u003Ca href=\u0022http:\/\/ecp.iedadata.org\/doidata\/10.1242\/jcs.01292\u0022 class=\u0022\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003E\u003Cimg src=\u0022http:\/\/ecp.iedadata.org\/doibanner\/10.1242\/jcs.01292\u0022 alt=\u0022\u0022 \/\u003E\u003C\/a\u003E \u003C\/div\u003E\n\n \n \u003C\/div\u003E\n\u003C\/div\u003E\n \u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\u003Cscript type=\u0022text\/javascript\u0022 src=\u0022http:\/\/jcs.biologists.org\/sites\/default\/files\/js\/js_hZg96SP9gBcOluDp2mGc57d8sP8uJ7g8P_JYsCISOgQ.js\u0022\u003E\u003C\/script\u003E\n\u003C\/body\u003E\u003C\/html\u003E"}