Re: [Histonet] Staining for Macrophages

From:

Gayle Callis

Sorry, but ED-1 (CD68) is a Mouse anti-Rat antibody for a subset of
rat macrophages. It may cross react to mouse but you would be doing
doing mouse on mouse IHC. The CD68 for mouse is rat anti-mouse,
clone FA-11 for murine peritoneal macrophages. SEROTEC is a source of
these antibodies, including the F4/80 (Rat anti-mouse).
A sidenote: SEROTEC has a free macrophage differentiation poster
available upon request.
At 06:58 PM 8/4/2004, you wrote:
You can use ED-1 from Serotec. It works on FFPE tissue with pH 6.0
citrate antigen retrieval and on frozen tissue without AR. You can
also use F4/80 on frozen tissue. Both are rat monoclonals and you
can use an anti-rat secondary Ab cross adsorbed against mouse IgG.
You could also also use ricin 1 from Vector or E-Y Laboratories
which binds mouse macrophages. Ricin 1 is not the dangerous
ricin. I don't know details of how to use it (I did once, but I
don't remember). There are books available or the manufacturers
may know or maybe some other Histonet folks. I have used ED1 and
F4/80 on mouse tissues and I think it's your best bet.
Sharon
I had previously asked about MAC-387 staining. Unfortunately, we
can't use the staining because we are staining mouse kidney and it
doesn't look right if you stain with a mouse anti-body. Does
anyone know of a way to stain for macrophages with out an
antibody??? Or perhaps a polyclonal antibody???? Thanks a bunch!
Jennifer K. Sipes, RALAT
Sr. Laboratory Technician
Johns Hopkins University
Ross 929
720 Rutland Avenue
Baltimore, MD 21205
phone: 410-614-0131
cell: 443-413-0853
e-mail: jengirl1014@yahoo.com
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--
Sharon Cooperman
NIH, NICHD, CBMB 301.435-7735
Building 18T, room 101 301.402-0078 fax
Bethesda, MD 20892
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
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