Triple Quadrupole Mass Spectrometer

Triple Quadrupole Mass Spectrometers are standard analytical tool for LC-MS/MS applications, in particular when accurate quantification is required.With a QQQ MS several types of experiments can be performed including:

MS2 scan

Product ion scan

Precursor ion scan

Neutral loss scan

Single Reaction Monitoring

Multiple Reaction Monitoring

Applications

QQQ mass analysis is highly suitable for high throughput detection and accurate quantification of compounds, especially in complex biological matrices.

At present BMSF has available analytical procedures for the quantification of non-reacted and reacted probes specific for the detection of superoxide anion radical (O2-.) and hydrogen peroxide (H2O2) in cells, tissues and other complex biological matrices.

Currently available methodologies make use of the following probes:

Hydroethidine (HE) - for detection of superoxide (O2-.)

Mito-hydroethidine (Mito-HE) – for detection of O2-. in mitochondria

Peroxy Green 1 (PG-1) – for detection of hydrogen peroxide (H2O2)

Mito-PY1- for the detection of H2O2 in mitochondria

Other analytical methods for the evaluation of oxidative stress include quantification of stable products resulting from the oxidation of proteins, lipids or DNA. Currently available methods allow for quantification of:

Chloro-tyrosine - a marker of HOCl-induced protein damage

F2-isoprostanes - a reliable indicator of oxidative stress status and inflammation in vivo