19 January 2012

Learn about a flexible, homogenous, no–wash assay for quantifying sirtuin activity in this application note. The SIRTainty™ Class lll HDAC Assay is coupled to nicotinamidase, which catalyzes breakdown of nicotinamide generated upon cleavage of NAD+ during sirtuin–mediated deacetylation of a substrate. Thus, the SIRTainty™ assay provides a direct assessment of the activity of class III HDAC enzymes.