Mannose/glucose-specific sophoragrin, a Sophora japonica bark lectin, shows unique multimerization and dissociation accompanied by transitions between insolubility and solubility, which are modulated by both a specific sugar and Ca^<2+> concentration. Sophoragrin is composed of four subunits, a-1, a-2, b-1, and b-2, and all except for b-2 are reported to be glycosylated. Biotinylated sophoragrin bound sugar-specifically to subunit 'b' but not to subunit 'a' on the membrane. Sophoragrin was labeled by only one of the photoaffinity-probes derived from sophoragrin-glycans, and the labeling was inhibited by a specific sugar. In contrast, another probe-mixture, which contains major oligosaccharide of sophoragrin (Man_3Xyl_1Fuc_1GlcNAc_2), did not label sophoragrin. These results reveal that the multimerization of sophoragrin is caused by a particular signal oligosaccharide on subunit 'b'. It was shown that sophoragrin can form a soluble complex with other endogenous glycoproteins, and may play a specific role through its transition between soluble and insoluble forms.

雑誌名

お茶の水女子大學自然科學報告

巻

53

号

2

ページ

45 - 52

発行年

2002-12

ISSN

00298190

書誌レコードID

AN00033958

フォーマット

application/pdf

形態

873181 bytes

日本十進分類法

400

出版者

お茶の水女子大学

資源タイプ

紀要論文

資源タイプ・ローカル

紀要論文

資源タイプ・NII

Departmental Bulletin Paper

資源タイプ・DCMI

text

資源タイプ・ローカル表示コード

03

所属

Department of Advanced Biosciences, Graduate School of Humanities and Sciences Ochanomizu University:(Present office)Department of Botany, Graduate School of Science, Kyoto University
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University
Department of Advanced Biosciences, Graduate School of Humanities and Sciences Ochanomizu University