In consultation with Paola R. Gomez-Pereira of the National Oceanography Centre, Southampton, we identified ''Oceanibulbus indoliflex'' and ''Roseobacter dentitrificans'' as promising chassis for the expression of our system.

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The roseobacter clade bacteria, RCB, constitute a significant proportion of coastal and mixed layer ocean bacterioplankton communities, upwards of 15 and 20% respectively, and are found in a diverse range of marine habitats. RCB demonstrate numerous traits, including aerobic anoxygenic phototrophy, aromatic compound degradation and recycling of sulphur within the water column and implication in carbon monoxide consumption,. (Wagner-Döbler & Biebl, ''et al.'' 2006). Significantly, in the context of plastic Island, they are shown to be major colonisers of submerged surfaces in marine waters. (Dang & Lovell ''et al.'' 2002).

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In consultation with Paola R. Gomez-Pereira of the National Oceanography Centre, Southampton, we identified ''Oceanibulbus indolifex'' and ''Roseobacter dentitrificans'' as promising chassis for the expression of our systems.

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The roseobacter clade bacteria, RCB, constitute a significant proportion of coastal and ocean bacterioplankton communities, estimated above 20% and 15% respectively, and are found in a diverse range of marine habitats. (Buchan ''et al.'' 2006). RCB demonstrate numerous traits, including aerobic anoxygenic phototrophy, aromatic compound degradation and recycling of sulphur within the water column and have been implicated in carbon monoxide consumption. (Wagner-Döbler & Biebl, ''et al.'' 2006). Significantly, in the context of plastic Island, they are shown to be major colonisers of submerged surfaces in marine waters (Dang & Lovell ''et al.'' 2002).

Contents

Marine Chassis

Roseobacter clade bacteria

In consultation with Paola R. Gomez-Pereira of the National Oceanography Centre, Southampton, we identified Oceanibulbus indolifex and Roseobacter dentitrificans as promising chassis for the expression of our systems.

The roseobacter clade bacteria, RCB, constitute a significant proportion of coastal and ocean bacterioplankton communities, estimated above 20% and 15% respectively, and are found in a diverse range of marine habitats. (Buchan et al. 2006). RCB demonstrate numerous traits, including aerobic anoxygenic phototrophy, aromatic compound degradation and recycling of sulphur within the water column and have been implicated in carbon monoxide consumption. (Wagner-Döbler & Biebl, et al. 2006). Significantly, in the context of plastic Island, they are shown to be major colonisers of submerged surfaces in marine waters (Dang & Lovell et al. 2002).

1uL DNA was added to 50uL competent cells to chilled 1mm electrocuvettes, and treated with a field strength of 2.5kV.

1ml marine broth was added to cells and mixtures were transferred to falcons for incubation overnight at room temperature at 200rpm and finally plated on marine agar supplemented with appropriate antibiotics. Incubated at 30˚C for 2 days.

Growth comparison

Growth comparison of Oceanibulbus indolifex and Escherichia coli in marine and luria broth.
We sought to compare the growth profiles of O. indolifex and E. coli.