It is widely accepted that ferrimagnetic -Fe2O3 has a spinel structure with vacancies in 1/6 of the 16 octahedral iron sites. Although there is consensus about this, maghemite structure seems tohave three possible unit cells: two cubic Fd-3m, P4132 and a tetrahedral P43212. Several experimental studies point to the three different space groups (227, 213 and 96 respectively) and itseems that the structure depends on two factors: the preparation method and the crystal size. However, the ordering of vacancies in any of those structures is still an unsolved issue. Currently,this point is quite difficult to tackle with computational modeling because superscells with at least three primitive cells are needed to represent an integer number of vacancies, which implies abig number of atoms for calculation. Moreover, the study of all possible configurations of vacancies implies an extremely large number of calculations to be performed. To our knowledge, atpresent only one work has been done for the space group P4132 using ab-initio calculations to determine the more stable configuration of vacancies. In this work, toward studying the magneticresponse of maghemite nanoparticles to be used in the Fischer Tropsch synthesis, we have used the Fd-3m structure to study the vacancy ordering, analyzing all non-equivalent configurations.We have chosen to describe the systems by density functional theory, using a well-established combination of plane waves and pseudopotentials with the LDA+U method. To simplify theproblem, we used the triclinic primitive cell, which contains 1⁄4 of atoms from the unit cell; we built a 1×1×3 supercell to obtain an integer number of vacancies. Using the SOD (Site OccupancyDisorder) software we determined all the 22 possible and not equivalent configurations for vacancies.