Removing lunar dust adhering to astronaut space suits is critically important for long-term lunar exploration. We are developing an automatic cleaning system that uses electrostatic force. It employs an alternating electrostatic field that forms a barrier on the surface of fabrics. In this study, we applied single-phase rectangular voltage to parallel wire electrodes stitched into the insulating fabric of space suits. By applying mechanical vibration and operating the system in a vacuum, we realized high performance: the cleaning rate exceeded 80%. Flicking out particles smaller than 10 μm in diameter that were trapped between fibers was difficult, but this system can perform preliminary space suit cleaning and save precious time for astronauts on the moon.