Hebrew University Researchers Succeed in Observing For 1st Time How DNA Damage is Identified in Living Cells

Protein (green dot) shown in this sequence scanning through a cell’s DNA for mutations.

For the first time anywhere, researchers at the Hebrew University of Jerusalem have succeeded in observing and describing how damaged DNA is naturally identified.

The research sheds new light on understanding this molecular mechanism and is likely to aid in research on diseases involving DNA damage, including cancer.

An article regarding the work of the Hebrew University researchers appears in the current issue of the scientific journal Cell.

The researchers, headed by Dr. Sigal Ben-Yehuda of the Department of Molecular Biology at the Hebrew University-Hadassah Medical School, revealed a new protein which scans DNA at the onset of bacterial sporulation. The protein moves quickly along the chromosome and identifies DNA damage. When the protein identifies such damage, it halts at that spot and signals to other proteins which repair DNA.

Under conditions of stress, some bacteria undergo a process of division which produces spores. These spores are particularly resistant to conditions of heat, radiation, dryness and exposure to chemicals, making it difficult to eradicate them with conventional methods, such as antibiotic drugs.

Most of the knowledge about sporulation of bacteria has been gathered over the years on a bacterium known as Bacillus subtilis, a bacterium which does not cause any illnesses. When this bacterium enters the sporulation phase, it verifies that the DNA sequence is in proper order and does not contain any mutations. But the process of how this occurs has not been observed until now.

“For the first time it is now possible to see how this phenomenon occurs,” said Dr. Ben-Yehuda. “Proteins triggered by the bacteria that are similar to the protein that has been revealed in our laboratory are found in all species, including humans, and therefore one can conclude that the way in which the bacterial protein scans the DNA for lesions is similar among many forms of life.

“This understanding of the molecular basis of the DNA repair is a basic step in furthering our ability to understand those illnesses stemming from DNA damage, for example cancerous growths.”