Goat anti Cat IgA (Fc specific)

The reactivity of the antiserum is restricted to the Fc part of the IgA molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal cat serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgA. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in cat serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of cat IgA from serum or plasma.

Host

Goat

Species Reactivity

Feline

Product Usage

Precipitating polyclonal Goat antiSerum to Cat IgA, Fc specific

Product Type

Secondary Antibodies

Applications

Immunoprecipitation

Fields of Interest

Veterinary

Product Source

Highly purified normal IgA isolated from pooled cat serum. FreundÕs complete adjuvant is used in the first step of the immunization procedure.

Product Formulation

Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.

Product Specificity

The antiSerum does not cross-react with any other component of the Cat immunoglobulin system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Storage Conditions

The lyophilized antiserum is shipped at ambient temperature and may be stored at +4¡C; prolonged storage at or below -20¡C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum.Diluted antiserum should be stored at +4¡C, not refrozen, and preferably used the same day. Lyophilized at +4¡ C--at least 10 years. Reconstituted at or below -20¡ C--3-5 years. Reconstituted at +4¡ C--7 days