Error Prone PCR

In article <392C05BA.4710D250 at biocomp.unl.edu>, Chris LaRosa
<clarosa at biocomp.unl.edu> wrote:
> Simon Margerison wrote:
> >
> > Hi
> >
> > A colleague of mine is interested in performing some error prone pcr.
> > Does anyone know any good methods or reagents for this. Any help would
> > be appreciated, either by direct email to
> >
> > ruth.featherstone at camb-antibody.co.uk> >
> > or on the board.
> >
>> Try using a higher concentration of dNTPs..... this will increase the
> errors. I know that a lower concentration of dNtps will give lower
> errors.
>> I am speculating here: limit the concentration of 3 of the dntps and
> increase the concentration of the remainder. This should cause more
> errors. How much so? Try a range of concentrations....
>> I make these comments,, haveing never done such an experiment, so take
> them with a grain of salt.
Try using polymerase which has the proofreading part missing. Such enzymes
are sold commercially by various companies. I have used one such enzyme
with success.