*P<0.05, compared with the control group; #P<0.05, compared with the blank and NC groups; measurement data were expressed as mean ± standard deviation; the data at different time points were assessed by repeated-measures analysis of variance. The experiment was conducted three times independently.

(A) Cell cycle entry in each group determined by PI staining of flow cytometry; (B) cell proportion in different stages in each group; (C) apoptosis of hepatocytes in each group detected by Annexin V-FITC/PI double staining of flow cytometry; (D) apoptotic rate of rats in each group; *P< 0.05, compared with the control group; #P<0.05, compared with the blank and NC groups; measurement data were expressed as mean ± standard deviation; the data were assessed by one-way analysis of variance. The experiment was conducted three times independently.

Figure 6RT-qPCR and Western blot analysis show that A20 inhibits activation of NF-κB signaling pathway in hepatocytes of ALF rats

(A) mRNA expressions of A20, NF-κB, TRAF6, and RIP1 in hepatocytes after transfection detected by RT-qPCR; (B) protein levels of A20, NF-κB, TRAF6, and RIP1 in hepatocytes after transfection detected by Western blot analysis; (C) protein band patterns of A20, NF-κB, TRAF6, and RIP1 in hepatocytes after transfection detected by Western blot analysis; *P<0.05, compared with the control group; #P<0.05, compared with the blank and NC groups; measurement data were expressed as mean ± standard deviation; the data were assessed by t-test. The experiment was conducted three times independently.