[0002]As diseases of domestic animals such as cattle, swine and chickens,
the incidence of calf scours, swine pleural pneumonia and atrophic
rhinitis, and chicken infectious bronchitis and colibacillosis are high.
Antibiotics and synthetic antibacterial agents are used as therapeutic
drugs for bacterial diseases among them, but resistant bacteria to these
antibacterial agents have appeared, and no sufficient therapeutic effect
has been obtained. In addition, public health problems have occurred
because the drugs used remain in the bodies of domestic animals. Thus,
preventive measures that do not depend on chemical therapy have been
desired.

[0003]Vaccines have been used for some viral diseases in domestic animals
and domestic fowl, but the effect of the vaccine is not sufficient as
seen in an example of chicken infectious bronchitis. Thus, the disease
still frequently occurs at present.

[0004]Domestic animals such as cattle, swine and chickens are often bred
in a narrow feedlot in high concentrations in consideration of their
early growth and breeding efficiency. Such a breeding method burdens
domestic animals with great stress. As with human beings, excessive
stress also brings an abnormality to the autonomic nervous system,
resulting in occurrences of problems such as immune abnormality including
allergic diseases, and constipation in domestic animals.

[0005]Also, constipation not only has a problem that a stool is not
excreted, but also if the constipation persists, the constipation itself
becomes stress to lose balance in the autonomic nervous system. As a
result, a sympathetic nerve tone is increased, and an immunological
capacity present in lymphocytes is reduced and disease (gastric ulcer and
the like) due to an increase in active oxygen occurs in some cases.

[0006]Therapeutic drugs for constipation include magnesium sulfate,
magnesium oxide and glauber's salt, but these have potential side
effects, e.g., if they are taken in a large amount, symptoms of poisoning
rarely occur and if they are taken in a large amount for a long period of
time, hypermagnesemia occurs. Also, phenolphthalein-based large intestine
stimulative purgatives include phenovalin, bisacodyl and sodium
picosulfate, but they have the potential for side effects such as nausea,
emesis and abdominal pain.

[0007]Also materials such as lactobacillus and dietary fibers derived from
safe foods are available, but their effects cannot always be said to be
sufficient, and new materials have been required.

[0008]Also, antihistamine agents, antiallergy agents and steroid agents
are used in order to improve diseases such as pollen disease, bronchial
asthma and atopic dermatitis due to a type I allergic reaction, but these
agents are sometimes associated with side effects, e.g., a condition
deterioration (rebound phenomenon) due to long term administration,
somnolence due to an action upon the central nerve and an effect on the
endocrine system through transdermal absorption.

[0009]Thus, for the purpose of safely improving allergic diseases without
any side effects, a drug containing rosmarinic acid extracted from a
Labiatae Plant as an active ingredient, although it is for human beings,
is described in Patent Document 1. A perilla extract obtained by removing
perillaldehyde and fractions of molecular weight of 10,000 or more from
an ingredient obtained by extracting from stems and leaves of the
Labiatae plant and treating it is described in Patent Document 2.

[0010]Patent Document 1: JP 1-121217-A

[0011]Patent Document 2: JP 7-215884-A

DISCLOSURE OF THE INVENTION

Problem to be Solved by the Invention

[0012]However, a drug having more effective drug efficacy than these
food-borne drugs using perilla extracts has been demanded.

[0013]The present invention has been realized in light of the above
points, and it is an object of the present invention to provide a
pharmaceutical composition having various drug efficacies.

[0015]Here, the powdered fennel is uikyou in Japanese Pharmacopoeia, one
kind of Chinese crude drugs, and has an action to discharge gas
accumulated in the intestine (carminative action), an action to
facilitate gastric movement and secretion of gastric juice (stomachic
action), an analgesic action, an expectorant action and an improving
action on apepsy.

[0016]Also, the powdered rhubarb is daiou in Japanese Pharmacopoeia, one
kind of Chinese crude drugs, and has a cathartic action, an antibacterial
action, a diuretic action and an antitumor action.

[0017]Further, the powdered glycyrrhiza is kanzou in Japanese
Pharmacopoeia, one kind of Chinese crude drugs, and has an antidotal
action, an antispasmodic action, a corticoid-like action, an inhibitory
action on gastric acid secretion, an expectorant action, an
anti-inflammatory action and an antitussive action.

[0018]Also, the powdered phellodendron bark is oubaku in Japanese
Pharmacopoeia, one kind of Chinese crude drugs, and has an antibacterial
action, an antimiotic action, an antiphlogistic action, a diuretic action
and a stomachic action.

[0019]Also, the powdered zedoary is gajutsu in Japanese Pharmacopoeia, one
kind of Chinese crude drugs, and has an absorption acceleratory action,
an antitumor action, an aromatic stomachic action and an anti-salmonella
enteritidis action.

[0020]Also, the powdered picrasma wood is nigaki in Japanese
Pharmacopoeia, one kind of Chinese crude drugs, and has an anthelmintic
action, a stomachic action and an antiprotozoal action.

[0021]Also, the powdered matricaria chamomilla is one kind of Chinese
crude drugs in Japanese Pharmacopoeia, and has an analgesic action, a
perspiration action, a carminative action, an antiphlogistic action, an
anti-cold action, an anti-rheumatoid action, an antidiarrheal action and
an anti-adenoiditis action.

[0022]Also, powdered geranium herb is one kind of Chinese crude drugs in
Japanese Pharmacopoeia, and has an antidiarrheal action and a stomachic
action.

[0023]Also, the powdered ginseng is ninjin in Japanese Pharmacopoeia, one
kind of Chinese crude drugs, and has a stimulant action on a nerve
system, a stimulant action on a pituitary-adrenal cortex system, an
augmenting action on sexual functions, a cardiotonic action, a lowering
action on blood sugar, an action to enhance digestion absorption and
metabolism to accelerate an appetite to promote protein synthesis, an
antidiuretic action, an antianaphylaxis action, an improving action on
asitia, an antidiarrheal action, a fatigue recovering action, an
improving action on nervous breakdown and a stomachic action.

[0024]Also, the powdered citrus unshiu peel is chinpi in Japanese
Pharmacopoeia, one kind of Chinese crude drugs, and has an expectorant
action, an antitussive action, a perspiration action and a stomachic
action.

[0025]Also, powdered scutellaria root is ougon in Japanese Pharmacopoeia,
one kind of Chinese crude drugs, and has an antipyretic action, a
diuretic action, an antibacterial action, an infection prevention action
against influenza (antiviral action), an antimiotic action, a sedative
action, an antihypertensive effect, an improving action on the asitia, an
analgesic action, an antidiarrheal action, an antiphlogistic action, an
antiemetic action and a constipating action.

[0026]Also, the powdered magnolia bark is kouboku in Japanese
Pharmacopoeia, one kind of Chinese crude drugs, and has an antibacterial
action, an antispasmodic action, a stomachic action, an anthelmintic
action, a diuretic action, an expectorant action, an antiemetic action
and a constipating action.

[0027]Also, the powdered oyster shell is kaki in Japanese Pharmacopoeia,
one kind of Chinese crude drugs, and has a sedative action, an analgesic
action, an astringent action, an antipyretic action and a tumor resolving
action.

[0028]Also, powdered cyperus rhizome is koubushi in Japanese
Pharmacopoeia, one kind of Chinese crude drugs, and has an analgesic
action, an antidepressant action, an analgesic action, an analgesic
action, an improving action on the asitia and an antibacterial action.

[0029]Also, the powdered platycodon root is kikyou in Japanese
Pharmacopoeia, one kind of Chinese crude drugs, and has an expectorant
action, an antiussive action, an anti-cold action, an antimiotic action
and an anti-salmonella enteritidis action.

[0030]Also, the powdered melia azedarach (kurenpi) is one kind of Chinese
crude drugs, and has an anthelmintic action and an constipating action.

[0031]Also, the powdered cnidium rhizome is senkyuu in Japanese
Pharmacopoeia, one kind of Chinese crude drugs, and has an antispasmodic
action, a sedative action, an antihypertensive action, a vasodilating
action, an antibacterial action and an antimiotic action.

[0032]Also, sodium bicarbonate is sodium bicarbonate in Japanese
Pharmacopoeia, and has an antacid action on gastritis and the like, an
improving action on acidosis and an acceleratory action on uric acid
excretion. Also, precipitated calcium carbonate is calcium carbonate in
Japanese Pharmacopoeia, and has an antacid action on gastritis and the
like and an improving action on hyperphosphatemia.

[0033]Also, the pharmaceutical composition of the present invention can be
used as a therapeutic drug for each of respiratory diseases, coccidiosis,
dermatomycosis, cryptosporidiosis, colibacillosis and viral infectious
diseases.

EFFECT OF THE INVENTION

[0034]The pharmaceutical composition of the present invention has various
drug efficacies.

[0036]In addition, each of 17 kinds of Chinese crude drugs is in a powder
form.

[0037]Also, the pharmaceutical composition of the present invention
includes various Chinese crude drugs, and thus includes various
glycosides. The glycoside included in the pharmaceutical composition of
the present invention includes, for example, a phenylpropanoid glycoside,
a glycoside of benzyl alcohol derivative, a phenylethanoid glycoside, an
anethole glycoside, a glycol glycoside, a monoterpenoid glycoside,
baicalin, glycyrrhizin, a phenylpropanoid glycoside, sennoside, a
flavonoid glycoside and ginsenoside.

[0038]Also, when the pharmaceutical composition of the present invention
is administered to an animal, the pharmaceutical composition is kneaded
with water of a small amount to form a paste, and the obtained paste is
adhered on or around the mouth of the animal, or mixed with a feedstuff,
or administered orally.

[0039]Also, 1 dosage of the pharmaceutical composition of the present
invention varies depending on the kind and weight of the animal. The 1
dosage is described below for each animal class and each weight class.

[0040]For example, 1 dosage of the pharmaceutical composition of the
present invention is 15 to 60 g for cattle weighing 300 kg or more, 7.5
to 30 g for cattle weighing 100 to 300 kg and 3.75 to 15 g for cattle
weighing 100 kg or less.

[0041]Also, for example, 1 dosage of the pharmaceutical composition of the
present invention is 3 to 12 g for swine weighing 100 kg or more.

[0042]Also, for example, 1 dosage of the pharmaceutical composition of the
present invention is 1.5 to 6 g for sheep and a goat weighing 30 to 100
kg, 1 to 4 g for sheep and a goat weighing 10 to 30 kg and 0.6 to 2.4 g
for sheep and a goat weighing 10 kg or less.

[0043]Also, for example, 1 dosage of the pharmaceutical composition of the
present invention is 0.15 to 0.6 g for a cat weighing 3 kg or more, 0.075
to 0.3 g for a cat weighing 1 to 3 kg and 0.0375 to 0.15 g for a cat
weighing 1 kg or less.

[0044]Also, for example, 1 dosage of the pharmaceutical composition of the
present invention is 10 to 40 g for a horse weighing 300 kg or more, 5 to
20 g for a horse weighing 100 to 300 kg and 2.5 to 10 g for a horse
weighing 100 kg or less.

[0045]Also, for example, 1 dosage of the pharmaceutical composition of the
present invention is 0.7 to 2.8 g for a dog weighing 20 kg or more, 0.35
to 1.4 g for a dog weighing 5 to 20 kg and 0.175 to 0.7 g for a dog
weighing 5 kg or less.

[0046]Also, for example, 1 dosage of the pharmaceutical composition of the
present invention is 0.07 to 0.28 g for a chicken in a late chick stage,
0.035 to 0.14 g for a chicken in an intermediate chick stage and 0.0175
to 0.07 g for a chicken in a young chick stage.

[0047]The pharmaceutical composition of the present invention blends 17
kinds of Chinese crude drugs as described above, and thus, ameliorates
the symptom in gastrointestinal diseases, gastrointestinal debility and
asitia and ameliorates the symptom in diarrhea, gastritis,
gastrointestinal ulcer, constipation and colic pain. Since further drug
efficacy can be expected, the pharmaceutical composition of the present
invention was used for respiratory diseases, coccidiosis, dermatomycosis,
cryptosporidiosis, colibacillosis, clostridiosis and viral infectious
diseases, and the presence or absence of its effect was confirmed.

[0048](Therapeutic Effect on Respiratory Infectious Disease)

[0049]The pharmaceutical composition (30 g) of the present invention was
administered twice daily to 9 growing Japanese Black Cattle aged 7 to 8
months and having respiratory symptoms, and their results were examined.

[0050]The body temperature during the first medical examination was
39.7±0.4° C. and the body temperature after two days was
39.3±0.4° C. A significant difference (p<0.01) was observed.
The body temperature became 38.9±0.3° after 4 days, and the
condition changed almost for the better.

[0051]The breathing rate during the first medical examination was
53.8±16.1 times/minute, and it became 45.0±11.7 times/minute on the
next day. A significant difference (p<0.05) was observed.

[0052]Subsequently, a change in the body temperature 2 hours after the
administration was observed in 9 Japanese Black Cattle aged 4 to 6
months. In 5 cattle to which the pharmaceutical composition of the
present invention was administered according to the above example of the
dosage, the body temperature was 39.6±0.6° C. before
administration and 39.7±0.5° C. after administration. No
difference was observed. In 4 cattle to which the pharmaceutical
composition of the present invention was administered in dosage twice the
aforementioned dosage, the body temperature was 39.8±0.4° C.
before administration and lowered to 39.6±0.6° C. A significant
difference (p<0.05) was observed.

[0053](Preventive Effect on Respiratory Infectious Disease)

[0054]Tilmicosin (3000 mg) was administered once to 16 cattle aged about
10 months introduced into a fattening farm around the same time and 15 g
of the pharmaceutical composition of the present invention was mixed with
their feedstuff twice daily continuously for a week (test cattle group).

[0056]1 week after the introduction, a fever (39.5±0.3° C.) was
observed in 2 cattle (12.5%) and purulent nasal discharge was observed in
6 cattle (37.5%) in the test cattle group. A fever (40.5±0.7°
C.) was observed in 6 cattle (15.0%) and purulent nasal discharge was
observed in 26 cattle (65.0%) in the control cattle group.

[0057]Also, An χ2 (chi-square) test was carried out for the
number of cattle with purulent nasal discharge, but no significant
difference was observed.

[0058]Therefore, the efficacy of the pharmaceutical composition of the
present invention for respiratory diseases was confirmed.

[0059](Therapeutic Effect on Coccidiosis)

[0060]The pharmaceutical composition (7.5 to 60 g) of the present
invention was orally administered once or twice daily to 4 cattle with
coccidiosis having bloody stools as a main symptom (1 Japanese Black Calf
aged 2 months and 3 fattening Japanese Black Cattle aged 20 to 24 months)
as the test cattle group. The number of coccidium oocysts (OPG) during
the first medical examination was made 100%, and its change was observed.
OPG during the second medical examination became 40% or less in all 4
cattle, and subsequently OPG was steadily reduced and the cattle were
cured.

[0061]As the control cattle group, Ektecin solution (7.5 g/100 ml of
sulfamonomethoxine and 2.5 g/100 ml of ormethoprim) which was a sulfur
agent was orally administered once daily (0.15 ml/kg) to 3 cattle with
coccidiosis having bloody stools as the main symptom, and their changes
were observed. OPG during the second medical examination became 50% or
less, and the cattle were rapidly cured.

[0062]Also, as the control cattle group, 30 g of Nutkin L (lactobacillus
4×1078/g, Bacillus subtilis 2×1078/g), which was an
attenuated vaccine, was orally administered once or twice daily to 2
cattle with coccidiosis having bloody stools as the main symptom, and
their changes were observed. OPG during the second medical examination
was 63.5% and OPG during the third medical examination was 80.4% in 1 of
the 2 cattle. In the other cattle, OPG during the second medical
examination was 77% and OPG during the third medical examination was 92%.
Thus, the pharmaceutical composition of the present invention was
administered during the third medical examination, and OPG during the
fourth medical examination was 20% or less in both the 2 cattle. They
were cured during the sixth medical examination. OPG was measured using a
ring method.

[0063]Therefore, the efficacy of the pharmaceutical composition of the
present invention for coccidiosis was confirmed.

[0064](Therapeutic Effect on Dermatomycosis)

[0065]An infusion of the pharmaceutical composition of the present
invention and a solution obtained by mixing the pharmaceutical
composition of the present invention with water was directly applied to 7
Japanese Black Calves aged 3 to 7 months with dermatomycosis. In 4 of the
7 calves, hair growth was observed within 1 month, and they were cured.
Also, the remaining 3 calves were cured within 1.5 months. Loss of scales
was observed within 15 days after the start of treatment in all of the
calves.

[0066]Therefore, the efficacy of the pharmaceutical composition of the
present invention for dermatomycosis was confirmed.

[0067](Therapeutic Effect on Cryptosporidiosis)

[0068]The attenuated vaccine alone was administered on the date of the
first medical examination and the second day to 1 of 7 cattle with
cryptosporidiosis having watery diarrhea, emesis and dehydration with
abdominal pain (case 1) and the pharmaceutical composition alone of the
present invention mixed with the feedstuff was administered from the
third day. The pharmaceutical composition alone of the present invention
mixed with the feedstuff was administered to the remaining 6 cattle.
Daily, 60 g of the pharmaceutical composition of the present invention
was administered.

[0069]As a result, the cattle in case 1 were cured on the sixth day after
the first medical examination. Among 6 cattle to which the pharmaceutical
composition alone of the present invention mixed with the feedstuff had
been administered, 3 were cured on the second day, two were cured on the
third day and 1 was cured on the fourth day.

[0070]Therefore, the efficacy of the pharmaceutical composition of the
present invention for cryptosporidiosis was confirmed.

[0071](Therapeutic Effect on Colibacillosis)

[0072]In order to examine the drug efficacy of the pharmaceutical
composition of the present invention for layers with colibacillosis, the
pharmaceutical composition of the present invention in a daily amount of
3 kg per 10,000 layers was mixed with the feedstuff, which was then
administered to the layers with colibacillosis for 5 days from Day 12 to
Day 16. Results are shown in Tables 1 and 2.

[0073]As is shown in Tables 1 and 2, the number of dead layers for 5 days
from Day 12 to Day 16 when the pharmaceutical composition of the present
invention had been administered and the number of dead layers after the
administration were lower in average than the number of dead layers
before the administration.

[0074]Therefore, the efficacy of the pharmaceutical composition of the
present invention for colibacillosis was confirmed.

[0075](Therapeutic Effect on Clostridiosis)

[0076]In order to examine the drug efficacy of the pharmaceutical
composition of the present invention for layers with clostridiosis, the
pharmaceutical composition of the present invention in a daily amount of
0.28 g per layer was mixed with the feedstuff, which was then
administered to the layers which had developed clostridiosis on Day 13
for 4 days from Day 20 to Day 23. For 1 day on Day 28, 10 kg of the
pharmaceutical composition of the present invention was mixed with the
feedstuff, which was then also administered to the layers.

[0078]As is shown in Tables 3 and 4, the number of dead layers with
clostridiosis was 20 or more except Day 16, but the number of dead layers
became less than 20 from Day 24 after administering the pharmaceutical
composition of the present invention for 4 days, and further the number
of dead layers did not exceed 15 after administering the pharmaceutical
composition of the present invention again on Day 28.

[0079]Therefore, the efficacy of the pharmaceutical composition of the
present invention for clostridiosis was confirmed.

[0080](Therapeutic Effect on Viral Infectious Disease)

[0081]The pharmaceutical composition (60 g) of the present invention mixed
with water was administered daily to 7 cattle exhibiting the symptom of
infectious diarrhea due to the infection with corona virus.

[0082]As a result, among the 7 cattle, 3 were given the pharmaceutical
composition of the present invention for 2 days and were cured 4 days
after the first medical examination, the other 3 were given it for 3 days
and were cured 5 days after the first medical examination, and the
remaining 1 was given it for 4 days and was cured 9 days after.

[0083]Therefore, the efficacy of the pharmaceutical composition of the
present invention for the viral infectious disease was confirmed.

[0084]As described above, the pharmaceutical composition of the present
invention blends 17 kinds of Chinese crude drugs, and thus has the
therapeutic effects on the respiratory disease, coccidiosis,
dermatomycosis, cryptosporidiosis, colibacillosis, clostridiosis and the
viral infectious disease, in addition to the ameliorating effects on the
symptoms in gastrointestinal diseases, the gastrointestinal debility and
the asitia as well as the ameliorating effects on the symptoms in
diarrhea, gastritis, gastrointestinal ulcer, constipation and colic pain.