Background of NALP2 antibody

NALP2 belongs to a family of cytoplasmic proteins that have been implicated in cell responses to apoptotic and inflammatory stimuli (1). Unlike the prototypical NALP protein NALP1, NALP2 only contains a NACHT domain, leucine rich repeat (LRR), and pyrin-containing domain (PYD) (2). This protein interacts with the adapter protein ASC in addition to CARD8 and caspase-1 to form an inflammasome with high proIL-1Β-processing activity and is thought to function as a modulator of NF-ΚB and procaspase-1 activation in macrophages (2,3). It has also been suggested that NALP2, in addition to other NALP family members, can act as innate sensors of pathogens in a manner similar to the toll-like receptors (TLRs) (4). At least two alternatively spliced transcript variants encoding distinct isoforms have been found for NALP2.

Total cell lysate from A549 cells (lane 1) or A431 cells (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-NLRP2 (clone 8F10B51) antibody. Proteins were visualized using an anti-mouse-IgG secondary that was conjugated to HRP and chemiluminescence detection.