Abstract

Selective suppression of rod signal transmission by cobalt ions was reported in carp retina. Using 10 μnol/L Co2+, rod-driven horizontal cells were hyperpolarized and light responses were completely suppressed in superfused, isolated retina, while cone-driven horizontal cells were almost unaffected. Similarly, scotopic electroretinographic bwave was suppressed by 10 μnol/L Co2+, while the photopic b-wave remained unaffected. Furthermore, the glutamate-isolated receptor potential (PIII) was not altered by low Co2+ under dark-adapted conditions. Other divalent ions with high affinity to calcium channels, such as cadmium and manganese ions, did not show similar suppressive effect on the rod horizontal cells. When rod horizontal cells were hyperpolarized by 10 μnol/L Co2+, the use of 3 mmol/L glutamate caused a significant depolarization of the cells, indicating that Co2+ application did not impair the ability of these cells to respond to glutamate. On the other hand, application of 200 μnol/L β-hydroxyaspartate, a glutamate transport blocker, mimicked the effect of low Co2+, suggesting a possibility that the low Co2+ effect might be related to a blockade of glutamate uptake by rods.

Keywords

rod system cone system electroretinogram horizontal cell cobalt ion

Project supported by the State Commission of Science and Technology of China, the National Natural Science Foundation of China, the National Eye Institute, the Human Frontier Science Program.