Mutation details: The vector contained a self-splicing floxed ACN cassette containing a neo and a cre recombinase gene and was inserted to replace exons 4-6. The final allele contains a sinlge loxP site in place of the exons as the neo and cre were deleted upon passage through the male germline. RT-PCR confirmed successful recombination of the locus in mutant kidney samples.
(J:122325)