I have a major problem with a western - I've applied primary and now secondary antibodies, but I've just discovered that the door code to the dark room has changed, everyone's gone home and I'm basically locked out. If I put the membranes in block overnight will a signal still be there tomorrow?

I suppose by now it's too late for any comments to be useful. I guess I would have preferred to leave it in 2nd Ab, but I imagine it was already too late for that when you discovered your predicament. If your Ab reactions are reasonably stable, you should have been OK going another 8 hrs in blocking solution. So how'd it come out?