Summary

Secondary Antibodies

Negative Isotype Controls

Useful Reagents

Positive Controls

Histology Controls

More Images

References

Reviews

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AHP639 is specific for human maternal embryonic leucine zipper kinase (MELK), also known as KIAA0175. MELK is a member of the AMPK family, which has several consensus glycosylation sites.

AHP639 consistently detects a band of approximately 120-150kD in Jurkat and Hela cell lysates, which is larger than the predicted size of 72kD. The activity of the antibody has been confirmed against recombinant MELK protein by western blotting.

Intended Use

Target Species

Human

Product Form

Purified IgG - liquid

Reconstitution

Preparation

Antiserum Preparation

Antisera to human MELK kinase were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Preservative Stabilisers

0.02%

Sodium Azide

0.5%

Bovine Serum Albumin

Immunogen

Peptide sequence KRLVEDILSSCKV corresponding to the C-terminus (CT) of MELK.

Purity

Approx. Protein Concentrations

IgG concentration 0.5 mg/ml

Reagents In The Kit

Preparing The Antibody

Test Principle

Buffer Solution

TRIS buffered saline

Storage

Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name

Yes

No

Min Dilution

Max Dilution

Western Blotting

0.5ug/ml

2ug/ml

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.