Description

Analysis Note

Sample preparation: The dust of a vacuum cleaner was sieved through a normal kitchen sieve. 500 mg of the sieved dust was suspended with 5.4 mL potassium hydroxide (10%) in methanol and stirred over 2h by 80°C.After cooling down, the suspension Â was centrifuged. 4mL of the obtained solution was filtered through a 0.45 μmÂ membrane filter and washed with 2mL water. The combined solutions were then extracted two times with 2 mL n-hexane. The n-hexane was evaporated with nitrogen gas and the residue was resolved in 1 mL methanol. TheÂ final solutionÂ wereÂ spiked with 100 μL of ErgosterolÂ (1 mg/mL) standard solution .

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