丙烯?屬氰化物之一，其所攜帶的?基不易為微生物所分解且對生物具有毒性。微生物轉換丙烯?是利用?水合?(NHase)將丙烯?專換至丙烯醯胺，再以醯胺?將丙烯醯胺轉換成丙烯酸與氨氮。其中間產物丙烯醯胺為具商業價值之物質，且有研究指出鈷或鐵離子為?水合?之輔助因子能增加其活性，而醛類能抑制醯胺?活。本研究分離出一株丙烯醯胺生成菌，經16S rDNA序列比對資料庫得知菌名為Mesorhizobium sp.；該菌株能以鈷離子作為?水點?之輔助因子，增加?水合?活性；當添加之丙烯?濃度由297.8mg/I增加至976.2mg/I時，丙烯?皆能完全轉換且隨丙烯?添加濃度愈高，丙烯醯胺累積濃度愈高，丙烯醯胺累積濃度由275.6mg/I增加至758.4mg/I。所添加丙烯?濃度提高至1906.0mg/I時，丙烯?轉換情形與丙烯醯胺累積濃度並不好，於實驗監測終點，丙烯?轉換率為30.2%，丙烯醯胺累積濃度為404.9mg/I；於醛類抑制實驗中，乙醛對於菌株Mesorhizobium sp.之醯胺?活性具有較佳抑制能力能幫助丙烯醯胺的累積，添加濃度493.8 mg/I之丙烯?其丙烯醯胺累積濃度為634.9 mg/I，但相對的卻會影響丙烯?之轉換情形；而丙醛抑制醯胺?能力較乙醛低，但卻具有較佳轉換丙烯?能力且能持續累積丙烯醯胺，於反應時間0.1小時，能將493.8 mg/I丙烯?完全轉換，且丙烯醯胺累積濃度為605.6 mg/I，丙烯?轉換丙烯醯胺百分率為91.7%。Acylonitrile is not easily decomposed by organisms and can cause servere environmental pollution due to its high toxicity and mutagenticity. The microorganisms that have both enzymes of nitrile hydratase (NHase) and amidase can catalyze the hydration of acylonitrile to acrylamide, and can further hydrolyze amide to acid and ammonia. Unlike amidase,nitrile hydratase is a metal-containing enzyme and requires the addition of metal ions to the culture medium for its activity. And some aldehydes including acetaldehyde, propionaldehyde, butyraldehyde, and acrylaldehyde can effectively inhibit the activity of amidase. The production of acrylamide is an important chemical which can be applied to manufacture of synthetic fibers, flocculant agests, paper making aids, and so on. The results indicated that one strain was isolated from the ployacrylonitriale(PAN) fiber manufacture wastewater treatment system. This strain was identified as Mesorhizobium sp. by methods based on 16S rDNA gene sequence. It produced significant levels of active nitrile hydratase when cobalt ion was added to the culture medium. When the acrylonitrile concentrations raised from 297.8mg/I to 976.2mg/I, Mesorhizobium sp. could remove arcylonitrile completely and accumulate acrylamide from 275.6 mg/I to 758.4 mg/I. But when the arcylonitrile concentration was 1906.0 mg/I, the removal efficiency was 32.2% in 118.8 hours, and the acrylamide concentration accumulated only 404.9 mg/I for the same period of time. The addition of acetabdehyde could inhibit the activity of amidase and accumulate more acrylamide, but it needed 126.4 hours to remove acrylonitrile completely. The inhibitiory effect of propionaldehyde was worse than acetaldehyde, but in the presence of propionaldehyde, acrylonitrile could be removed completely within 0.1 hours and acrylamide could accumulate to the concentration of 605.6 mg/I, the percentage of conversion was 91.7%.