"... I am truly indebted and thankful to my supervisor, Peter Bühlmann. He saved my hide countless times with advice, ideas and his calm sense of dealing with people and situations – which ultimately resulted in the successful completion of my PhD. However, it was these other moments which made my time ..."

eagerly. I hope he will put his rescue tool in good use. I thank Prof. Lars Hennig and Prof. Marloes H. Maathuis for acting as co-examiners and for the constructive research collaboration in the last three years which they made possible. Their thoughts and com-ments allowed me to overcome many gordian

for proteomic analysis. dDAVP and control samples were labeled with different cleavable ICAT reagents (mass difference 9 amu) and mixed. This was followed by 1-D SDS-PAGE separation, in-gel trypsin digestion, biotin-avidin affinity purification, and LC-MS/MS identification and quantification. Responses

"... Many important areas of research regarding human health, such as immunology and cancer biology, deal with highly heterogeneous populations of cells where the contributions of individual players cannot be ignored. Single-cell technologies aim to resolve this heterogeneity by analyzing many individual ..."

individual cells in a high-throughput manner. Here we developed two examples of such tools that rely on microfabricated arrays of microwells. The first platform merges fluorescence cytometry with label-free profiling of the small molecule composition of tens of thousands of cells based on matrix

"... Part of the Biology Commons, Cell Biology Commons, and the Molecular Biology Commons This Dissertation is brought to you for free and open access by the Graduate School at Scholar Commons. It has been accepted for inclusion in Graduate Theses and Dissertations by an authorized administrator of Schol ..."

Part of the Biology Commons, Cell Biology Commons, and the Molecular Biology Commons This Dissertation is brought to you for free and open access by the Graduate School at Scholar Commons. It has been accepted for inclusion in Graduate Theses and Dissertations by an authorized administrator of Scholar Commons. For more information, please contact