FAAH is a serine hydrolase enzyme of the endocannabinoid system which is responsible for the catabolic inactivation of the neuromodulator anandamide. FAAH inhibitors cause a build up of anandamide, which acts through activation of cannabinoid receptors to promote analgesia. Percentage inhibition data is reported in WO2010074588 [1], with rat brain FAAH inhibited by 78.2% at 100nM compound in vitro.BIA 10-2474 does not interact with other proteins of the endocannabinoid system or with the endocannabinoid-binding transient receptor potential (TRP) ion channels [6].