Bats are the only mammals capable of true flight. However, there are several gliding mammals which are able to glide from tree to tree using fleshy membranes between their limbs; some can travel hundreds of meters in this way with very little loss in height. Flying tree frogs use greatly enlarged webbed feet for a similar purpose, and there are flying lizards which employ their unusually wide, flattened rib-cages to the same end. Certain snakes also use a flattened rib-cage to fly, with a back and forth motion much the same as they use on the ground.

Flying fish can glide using enlarged wing-like fins, and have been observed soaring for hundreds of meters using the updraft on the leading edges of waves. It is thought that this ability was chosen by natural selection because it was an effective means of escape from underwater predators.

Most birds fly (see bird flight), with some exceptions. The largest birds, the ostrich and the emu, are earthbound, as were the now-extinct dodos, while the non-flying penguins have adapted their wings for use under water. Most small flightless birds are native to small islands, and lead a lifestyle where flight would confer little advantage. The Peregrine Falcon is the fastest animal in the world; its terminal velocity exceeds 370 km/h (199 mph) in a dive.