Although the Natural Park has lost a high percentage of its varied fauna in relatively few years due to human influence, there are numerous species of birds and animals living in the area. Camouflaged against the woods and thickets are the deer, the largest and most characteristic mammal, and the wild boar, whose populations are often protected and controlled in extensive hunting reserves. The Iberian Lynx is sadly extinct from the area (although there are plans for reintroduction), but there are a number of other carnivorous land mammals, such as foxes, badgers, mongeese, wild cats, polecats and genets.

In the clear waters of the rivers flowing through the Sierra Norte de Sevilla Natural Park there is a thriving population of otters, the existence of which is linked to the existence of fish species, including the only rainbow trout in the province. The El Pintado reservoir is also home to other species, including the catfish and barbel.

However, it is birds that have best adapted to the terrain, and make up the numerous mammal species in the Natural Park due in no small part to the variety of habitats. Of birds of prey, there are nesting pairs of golden eagle, plus the black vulture, the Spanish imperial eagle and the buzzard, amongst others. It is relatively easy to spot black storks flying above the plains, and although much more difficult to spot in dense vegetation, the spectacular golden oriole is also resident in the area. Due to the surface rivers and reservoir expanses, water birds such as the kingfisher, herons, cranes and spoon-billed duck are common.