In HeartGold and SoulSilver, the girls' respective Eeveelutions were swapped; only Zuki and Kuni's partners remained the same from Generation II. Additionally, Kuni's Japanese name, Sumomo, was altered to Komomo, probably to avoid confusion with Maylene, whose Japanese name is also Sumomo.

Quotes

Zuki

Before battle

"Welcome. My name is Zuki. I met you previously in Violet City. In order to bring back Ho-OhHG/LugiaSS, we needed someone with the right bond with Pokémon. We asked Mr. Pokémon to give this Mystery Egg to whoever showed true potential. The Egg was eventually handed from Mr Pokémon to Professor Elm, and then to you. However, being pure alone will not be sufficient to face the power of this Pokémon. I must test you. Are you ready?"

"I will test how closely you've bonded with your Pokémon!"

Being defeated

"I don't have any Pokémon left."

Naoko

Before battle

"You are quite strong. You helped me in the Ilex Forest. My name is Naoko. I'm the one that's not so good at directions. My eyes witnessed you defeating Team Rocket when Kurt was in trouble at the Slowpoke Well. Allow me to challenge you and your Pokémon!"

Being defeated

"Oh, you are truly strong."

Miki

Before battle

"You are quite the Trainer. I remember you chasing off that Team Rocket Grunt from right here. I must thank you for that. Although, I am actually a Trainer myself. She who knows the most speaks the least! I am Miki--the Kimono Girl! Allow me to challenge you and your Pokémon!"

Being defeated

"Ooh, you're good at Pokémon, too."

Sayo

Before battle

"Excellent fighting spirit! You got me off the ice by pushing me from the back. I am the one who skates in sandals. I am Sayo--the Kimono Girl! I was listening in the Dragon's Den when you passed the test of the Elder. Allow me to challenge you and your Pokémon!"

Being defeated

"Oh, so close! I almost had you."

Kuni

Before battle

"I am the last one. You were so dependable at the Goldenrod Tunnel. I am the one who twirls even underground. I am Kuni--the Kimono Girl! I looked at you in wonder when you thwarted Team Rocket's mission to take over the Radio Tower. You must show your strength to me now. Allow me to challenge you and your Pokémon!"

In the anime

The Kimono Sisters in costumes in the anime.Left to right: Sumomo (in green), Tamao, Satsuki, and Koume

The Kimono Sisters in casual clothes. Left to right: Koume, Sumomo, Satsuki, and Tamao

The Kimono Girls (called the Kimono Sisters in the anime) were featured in two episodes, Trouble's Brewing and Espeon, Not Included. The dub retained their original Japanese names instead of using their localized English names.

In Trouble's Brewing, after Ash's win at the Ecruteak Gym, the gang bumped into Sakura, who took them to the tea ceremony her sisters were running when they ask where it was being held. Sumomo was the first sister they met, greeting them formally. Tamao was seen next, demonstrating ikebana to a small audience. Later, they passed by the lone Koume playing her shamisen. Finally, they were treated to a quiet display of the art of tea making and drinking by Satsuki. Needless to say, Brock was entranced by them all and grew frightened when they were all together and called him out on his praise.

The sisters are skilled Trainers as well as tradition keepers. Upon proposing a challenge to the trio to battle for Sakura to join them, Sumomo and Satsuki both displayed their Vaporeon's and Jolteon's abilities against Misty's Poliwhirl and Ash's Pikachu respectively. Vaporeon defeated Poliwhirl and Pikachu defeated Jolteon, but the latter only after Team Rocket's interruption.

When Sakura changed her mind about leaving at that time, Satsuki admitted that she and the other three weren't against Sakura leaving to begin her journey, only that they wanted her to not be dependent on others if she finally did.

In Espeon, Not Included, the four elder sisters were tricked by Team Rocket into handing over their Pokémon for dancing lessons. With the help of Sakura's Eevee, now an Espeon, the other four Eeveelutions were rescued, and the episode ended with the sisters wishing Sakura well on her journey.

Pokémon

Jolteon

Satsuki's Jolteon was first seen when Team Rocket disrupted the Kimono Sisters' tea ceremony, but didn't battle until she matched up against Ash and Pikachu. It fended off a few attacks from Pikachu, but ultimately succumbed to a Thunder attack.

Sumomo's Vaporeon was first seen when Team Rocket disrupted the Kimono Sisters' tea ceremony, but didn't battle until it matched up against Misty and Poliwhirl. The Pokémon were well matched, but Vaporeon defeated Poliwhirl with its Aurora Beam.

The Kimono Girls make their debut in VS Parasect, when Gold decides to visit them in Ecruteak after finishing up at the Pokéathlon. Gold is spending his time watching them perform, which annoys the Radio Director of Goldenrod. However, Gold reveals that he is actually allowing time for Aibo to train, who was trying to learn Double Hit. When he finally does, Aibo evolves into Ambipom, winning the praise of the Kimono Girls.

Trivia

For some reason, the Kimono girls were given different but still Japanese names in the English versions. The French localization also followed suit, but were renamed to their original Japanese names in Generation IV with the exception of Koume, who was referred to as Umeko instead.

In the French and German localizations of Gold, Silver, and Crystal, the name Keiko was used for different Kimono Girls.

In HeartGold and SoulSilver versions, when the Kimono Girls are summoning the legendary Pokémon, they dance clockwise for Ho-Oh and counter-clockwise for Lugia.

Both of the anime episodes which featured all of the Kimono Sisters had Masaaki Iwane as animation director.

In the anime episode Espeon, Not Included, the four elder sisters' fans match the elemental themes of their Pokémon.

In the anime, all of the Kimono Sisters except for Sakura have their Eeveelutions in an Apricorn Ball; in the games, these, along with the Safari Ball and the Sport Ball, are the only Poké Balls that an Eeveelution cannot be legitimately caught in.