Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Applications

Approved:

IHC - Paraffin (1:50)

Flow Cytometry (1:1 - 1:10)

Specificity and Use

Immunogen:

CD44 antibody was raised against human T lymphocytes

Specificity:

Recognizes the human CD44 cell surface antigen, a 100kD glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and of the breast. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.

Usage:

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

Presentation:

PBS, pH 7.4, 0.09% sodium azide, 1% BSA.

Storage:

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles and prolonged exposure to light.

Description: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

Immunohistochemistry

Anti-CD44 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5963 dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.