Top Questions

What DsiRNA concentration do I need to use in my experiment to see knockdown?

For an RNAi or antisense experiment, the actual level of target gene knockdown is related to the transfection efficiency. A positive control such as HPRT DsiRNA should be used in each experiment to assess transfection efficiency. In addition, IDT recommends using a dose-response curve of 0.1, 1, and 10 nM to determine maximum response.