Environmental manipulation to increase the nutritional content in leafy vegetables

by Lefsrud, Mark Gregory.

Abstract (Summary)

In plants, carotenoids play critical roles in both light harvesting and energy
dissipation for photosynthetic mechanism. In humans, carotenoids have been
associated with reduced risk of lung cancer and chronic eye diseases, such as
cataracts and age-related macular degeneration. Increasing carotenoid levels in
plants, that are commonly consumed in the diet would impart health benefits without
changing the dietary habits of individuals.
Kale (Brassica oleracea L. var. acephala D.C.) ranks highest and spinach
(Spinacia oleracea L.) ranks second among vegetable crops for the accumulation of
the carotenoids, lutein and ?-carotene. However, kale has low consumption rates,
while spinach has one of the highest rates of consumption among green-leafy
vegetables in the United States. Limited research is available on the impact of
environment and drying method on the production and destruction of secondary plant
compounds. Therefore, the objectives of these studies were to determine how leaf
age, light (irradiance, photoperiod, radiation cycle and wavelength), nutrition
(nitrogen and selenium), and air temperature influences the accumulation of
carotenoids, chlorophylls and dry matter in kale and spinach.
Data from the nitrogen nutrition experiment revealed that increased growth
through fertilization resulted in increased lutein and ?-carotene when measured on a
fresh mass basis for one variety of spinach. However, when carotenoid accumulation
was measured on a dry mass basis, both varieties were significantly affected by
additional nitrogen. Results from this study showed that to accurately understand
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carotenoid accumulation, values need to be analyzed on both a fresh and dry mass
basis. Further studies measuring the influences of air temperature, irradiance, leaf
age, photoperiod, radiation cycle and wavelength showed that maximum biomass did
not always correlate with maximum carotenoid accumulation. A further study
demonstrated that selenium fertilization did not affect carotenoid accumulation in
kale; therefore kale could be used to provide both selenium and carotenoids in human
diets.
When plant pigment concentration were analyzed in relation to % dry matter
(%DM) a linear trend was observed. Increases in %DM of the plant resulted in
measured increases in fresh mass pigment concentration and decreases in dry mass
pigment concentration. Further analysis showed that %DM could be used to explain
approximately 40% of the variance in the pigment concentration. A final study was
performed to determine the effect of drying method and drying temperature on
pigment concentration. Drying method and temperature had no effect on pigment
concentration when the drying temperature remained below +25ºC; at temperatures
above +25ºC some destruction of the pigments and loss of plant biomass was
observed.
Environmental factors can be used to control carotenoids, chlorophylls and
%DM in both kale and spinach. Utilizing these cultural practices is important
information for growers producing these crops for dry capsule supplements and fresh
markets.
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