Thank you. 25 mg represents a 3X overdose but isn't expected to cause more than gastrointestinal distress - vomiting and/or diarrhea in addition to its diuretic effect. Make sure water is available and an electrolyte replacer such as the infant Pedialyte (or generic) added to her water will be helpful in preventing acute electrolyte (sodium, potassium) imbalances. The pharmacokinetics of this drug haven't been studied in dogs but you can expect a diuretic effect for up to 12 hours.