Two major highways, Interstates 580 and 680 cross the Tri-Valley area. Interstate 580 runs east-west, connecting the area to the Central Valley to the east and the San Francisco Bay area in the west. Interstate 680 runs north-south, connecting the area to the San Francisco Bay area in the south, and the Concord-Walnut Creek area in the north. The two interstates cross on the border of Dublin and Pleasanton.

The Tri-Valley area is also serviced by BART, offering service to the San Francisco Bay Area by way of Oakland, with two stops: West Dublin/Pleasanton and the Dublin/Pleasanton terminus station. The West Dublin/Pleasanton Station opened in 2011, and was the first infill station on an existing line. BART plans include eventually extending the line to Livermore in the near future, and possibly into the Central Valley at Tracy in the distant future.