LEFT VIDEO: Time-lapse TIRF microscopy movie corresponding to multiple particle tracking in untreated normoxic HeLa cells after incubation with compound 10 (100 uM). Time is indicated as seconds and images were acquired every 50 ms and played at 30 frames per second. Scale bar: 5 µm.

RIGHT VIDEO: Time-lapse TIRF microscopy movie corresponding to multiple particle tracking in DMOG-treated hypoxic HeLa cells after incubation with compound 10 (100 uM). Time is indicated as seconds and images were acquired every 50 ms and played at 30 frames per second. Scale bar: 5 µm.

RIGHT VIDEO: Time-lapse video showing mCherry-expressing macrophages at the wound edge within 4 h post wounding and 30 min of compound 5 administration. White arrows point at only few macrophages containing fluorescent phagosomes. Macrophages are still actively engulfing and moving, suggesting that they are still viable at this stage. Scale bar: 50 μm

LEFT VIDEO: Time-lapse high-resolution imaging of A. fumigatus upon treatment with the peptide 8. A. fumigatus were pre-treated with a cell membrane counterstain (red signal) and imaged under confocal microscope. After 20 s, cells were treated with the peptide 8 (2 μM, green signal) and further imaged without any washing. The movie shows the rapid fluorogenic response of 8 upon interaction with the fungal cell membrane and subsequent internalisation in lipophilic environments. Scale bar: 2.5 μm.

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CENTER VIDEO: 3D projection of fluorescence images of A. fumigatus after incubation with the peptide 8. Peptide 8 (2 μM) was incubated for 15 min in A. fumigatus that had been not pretreated with PAF26, and cells were imaged under a confocal microscope at 37 °C. Scale bar: 10 μm.

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RIGHT VIDEO: 3D projection of fluorescence images of PAF26 pre-treated A. fumigatus after incubation with the peptide8. Peptide 8 (2 μM) was incubated for 15 min in A. fumigatus that had been pre-treated with PAF26 (3 μM) for 30min, and cells were imaged under a confocal microscope at 37 °C. Scale bar: 10 μm.

Multicomponent Reactions for de Novo Synthesis of BODIPY Probes: in vivo Imaging of Phagocytic Macrophages.

RIGHT VIDEO: Time-lapse movie of a single macrophage in the flank region of a 3 dpf Tg(cfms:mCherry) larva after incubation with PhagoGreen. The movie shows the strong green signal of PhagoGreen only in mature phagosomes (i.e. phagosomes upon acidification) compared to the non-stained newly formed phagosomes (yellow arrows). Scale bar: 20 μm.