Tween-20 Activates and Solubilizes the Mitochondrial Membrane-bound, Calmodulin Dependent NAD+...
Pou de Crescenzo, M.-A. ; Gallais, S. ; Léon, A. ; Laval-Martin, D.L.
2001-07-15 00:00:00
Among different treatments assayed, a mix of a nonionic detergent (5% Tween-20) with 0.5 m NaCl was found to solubilize a large part of the calmodulin-dependent NAD+ kinase bound to the inner mitochondrial membrane. It also stimulated its activity by increasing 7 times the maximal velocity. Activity stimulation was also observed with phosphatidylcholine, phosphatidylethanolamine and with reductants (HSO3 and DTT). This solubilized NAD+ kinase and the calmodulin-dependent cytosoluble isoform displayed distinct molecular masses, as well as different kinetic parameters. We propose that solubilization of membrane-bound NAD+ kinase could occur in vivo in Avena sativa and could generate a soluble isoform.
http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.pngThe Journal of Membrane BiologySpringer Journalshttp://www.deepdyve.com/lp/springer-journals/tween-20-activates-and-solubilizes-the-mitochondrial-membrane-bound-pUjxkPF76x

Abstract

Among different treatments assayed, a mix of a nonionic detergent (5% Tween-20) with 0.5 m NaCl was found to solubilize a large part of the calmodulin-dependent NAD+ kinase bound to the inner mitochondrial membrane. It also stimulated its activity by increasing 7 times the maximal velocity. Activity stimulation was also observed with phosphatidylcholine, phosphatidylethanolamine and with reductants (HSO3 and DTT). This solubilized NAD+ kinase and the calmodulin-dependent cytosoluble isoform displayed distinct molecular masses, as well as different kinetic parameters. We propose that solubilization of membrane-bound NAD+ kinase could occur in vivo in Avena sativa and could generate a soluble isoform.