In 2369, CaptainEdward Jellico compared Cardassians to the wolf-subspecies known as timber wolves, saying that they possessed an instinctive need to establish a dominant position in social situations, and thus contrived his strategy for negotiating with GulLemec accordingly. CounselorDeanna Troi reminded him that sometimes a wolf ended up dead in the fight for dominance, to which Jellico responded by saying that the trick was to be the wolf that was standing in the end. (TNG: "Chain of Command, Part I")

When Deanna Troi entered Lwaxana Troi's mind to find out why her mother was unconscious, an angry wolf tried to keep her away from the memories of Kestra Troi. (TNG: "Dark Page")

In an ultimately unused line of dialogue from the final draft script of TOS: "Charlie X", Spock recalled that, in the Human legend of Romulus and Remus, a wolf looked after the infants, who were otherwise left to fend for themselves in the wilderness.

It is common misconception that the star Wolf 359 was named after wolves. It was actually named after the Earth astronomer Max Wolf.