The unequivocal detection of entanglement between two distinct matter-wave pulses is a significant challenge that has yet to be experimentally demonstrated. We describe a realistic scheme to generate and detect continuous-variable entanglement between two atomic matter-wave pulses produced via degenerate four-wave mixing from an initially trapped Bose-Einstein condensate loaded into a one-dimensional optical lattice. We perform a comprehensive numerical investigation for fixed condensate parameters to determine the maximum violation of separability and Einstein-Podolsky-Rosen inequalities for field quadrature entanglement, and describe and simulate an experimental scheme for measuring the necessary quadratures.