HPLC Method Development (1 day)

Step by step, the course introduces all important foundations and the tools to perform an independent method development including column screening.

Target audience

The course is suitable for users with basic HPLC and very basic natural science knowledge.

Goal of the training

This course starts with theoretical background in chemistry and physics, which are important for success in method development. The course participant will learn to gain information already from the molecule structure or what to do if there is no information about the analyte. Afterwards, all steps from planning the method development to a robust method will be described and discussed. At the end of the course, the participant will be able to independently plan and perform a method development.

Topics

Molecule structure

Pilot tests

Column types

Limits of the mostly used column materials

NP or RP

Instrumental requirements for a column screening

Step by step column screening procedure

Chemical and physical adjusting capabilities

Finding the best combination of parameters

Robustness of a method

You may also be interested in our other courses to deepen your knowledge in HPLC troubleshooting, method development and ClarityChrom.
Book our HPLC all-in-one KNAUER holiday package including the HPLC Basic Training, HPLC Troubleshooting, Method Development and the ClarityChrom Software Training for € 2.500,- instead of € 3.100,- p.p.

Training dates and prices

Training location

The trainings take place in the KNAUER headquarters. Our modern training facilities include bright rooms and a fully equipped laboratory for the workshops. During the breaks you can enjoy the KNAUER garden and the quiet surroundings of the company.

The KNAUER headquarters is located in the south of Germany's capital city Berlin. Before or after the training you can visit numerous tourist attractions either in the buzzing metropole Berlin or in the culture center Potsdam, which only a short train ride away. Sights like Brandenburg Gate, the Reichstag building, Checkpoint Charlie, the TV Tower, the UNESCO world heritage Sanssouci Palace and more than 170 museums are only some of the possibilities to spend your free time.

General course information

KNAUER provides a wide spectrum of application training in the various aspects of chromatography. The courses are designed to provide a learning experience for HPLC and FPLC beginners, users and experts. There are hands-on training courses HPLC basics, troubleshooting, method development, FPLC troubleshooting, software training, SMB basics, SMB method development and Prep LC basics. The courses are run at the KNAUER headquarters in Berlin or customized at your premises.

Cancellation policy
In case you need to cancel your registration, the following charges will apply:

• 30 to 21 days prior to course: 50% of course fee
• 20 to 8 days prior to course: 80% of course fee
• 7 days or less prior to course: 100% of course fee

If you are unable to attend after registration, you may send a colleague in your place or attend another course withour additional costs.
KNAUER reserves the right to modify course material, substitute speakers, or to cancel the course. If the course is cancelled, registrants will be notified as soon as possible, latest 30 days in advance and will receive a full refund of paid fees. KNAUER will not be responsible for airfare penalties or other costs incurred due to a course cancellation.

Course certificate
Upon completion of the course, each participant receives a course certificate in which course name and course date is stated.

Course evaluation
At the end of each course, you will be asked to fill in a course evaluation form. We value your opinion of the course, the speakers, the material, and presentations and use this feedback to continuously improve the courses and their contents.

Travel and hotel costs
Travel and hotel costs are not included in the course price.

Language
Standard courses are held in German and upon request in English. Customized courses can be presented in other languages. Please contact the KNAUER academy for more information.

Time tableAll courses take place from 9 a.m. to 5 p.m.

Lunches
All lunches during course days are included in the course prices.

Material in binders
Each course participant will receive the lectures and other relevant material in a binder.

Requirements for safety
Every course participant who enters our laboratories for the practical sessions must comply with certain safety requirements. Please notice that open-toe shoes are not allowed in the lab. Obligatory protective clothing and safety devices will be provided.