bicfo at flinders.edu.au wrote:
>Gidday, fellow netters!
>I'm having truckloads of trouble trying to consistently recover DNA
>(0.7-1.8kb) from low-melting point agarose gels. I've tried agarase digestion,
>phenol extraction, Promega WIZARD PCR columns and 'squeeze-freeze'
>techniques - all with limited success (none at all with the agarase!). Does
>anybody have a foolproof method, or can anyone direct me to somewhere on the
>net where different techniques might be discussed?
>Thanks, Colleen.
Qiagen has a rapid spin column tht is specifically designed for this purpose. I
haven't tried this product but commonly use other columns they produce and I've
found them to be second to none.
Leon