Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

adenocarcinoma

Age

31 years

Gender

female

Ethnicity

Black

Applications

The H-Hela cell line was established by V. Hamparian from the HeLa cell line isolated by Gey, et al.

This subline is susceptible to rhinoviruses and is useful for passaging and titrating rhinoviruses.

Derivation

The H-Hela cell line was established by V. Hamparian from the HeLa cell line isolated by Gey, et al. (see ATCC CCL-2).

Clinical Data

31 years

Black

female

HeLa Markers

Y

Genes Expressed

keratin

Cellular Products

keratin

Comments

The H-Hela cell line was established by V. Hamparian from the HeLa cell line isolated by Gey, et al. (see ATCC CCL-2).

This subline is susceptible to rhinoviruses and is useful for passaging and titrating rhinoviruses.

The line is usually carried as monolayer cultures in Leibovitz's L-15 medium, but can be readily adapted to other media and to suspension culture (in media without Ca++).

The H-HeLa line was cured of a mycoplasma contamination by W.M. Lee in Rueckert's laboratory and the cured line was given the designation H1-HeLa.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)

Subculturing

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.