GC-Rich PCR Amplification—Advantage GC 2 Polymerase Mix & PCR Kit

Advantage GC 2 Polymerase Mix is an excellent choice for amplifying complex, GC-rich templates, such as the 5' ends of many mammalian cDNAs, that cannot be amplified using conventional PCR techniques. Advantage GC 2 includes Advantage 2 Polymerase Mix, our proprietary GC-Melt Reagent and a reaction buffer containing DMSO—two proven additives allowing amplification of sequences that are up to 90% GC with minimal buffer optimization.

When used together, DMSO and GC-Melt reagent allow the amplification of virtually all GC-rich sequences that are resistant to PCR amplification by standard techniques. The Advantage GC 2 Polymerase Mix and PCR Kit are ideal for use with cDNA templates. For amplifying regions of GC-rich mammalian genomic DNA, we recommend using Advantage GC Genomic LA Polymerase Mix (Cat. No. 639153).

Features

Optimized for use with complex, GC-rich cDNA and genomic DNA templates

Generates virtually error-free PCR products

Amplifies targets of up to 6 kb

Applications

GC-rich PCR

Long and accurate PCR

Hot start PCR

Difficult target PCR

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.