MHC Class I H-2b/D/P/Q/w16 Antibody | ER-HR52

Published customer image:Rat anti Mouse MHC Class I H-2b/D/P/Q/w16 antibody, clone ER-HR52 (MCA2398) used for the identification of MHC class I expressing cells in mouse brain by immunofluorescence.Image caption:Dynamic expression of H-2Kb/Db in mouse hippocampus.(A) At P15, MHCI protein signals (green) were overlapped with those of NeuN (red, a marker for neurons) but not with GFAP (red, a marker for astrocytes). Scale bar: 50 µm. (B) The mRNA of H-2Kb and H-2Db was dynamically expressed in mouse hippocampus. Data was shown as ratio compared with P4 and was calculated from three independent experiments. (C) Western blot analysis depicting the expression of MHC-I protein during the development stages of hippocampus. (D) Expression of MHC-I protein was quantified as the ratio of band density to that of Tuj1. Data was presented as ratio compared with P4 and was calculated from three independent experiments.

Cells from immunised rats were fused with cells of the mouse P3-X63-Ag8.563 myeloma cell line.

Storage

Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.

Shelf Life

18 months from date of despatch.

Acknowledgements

Regulatory

For research purposes only

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name

Yes

No

Min Dilution

Max Dilution

Flow Cytometry

1/50

1/200

Immunohistology - Frozen

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Technical Advice

Recommended Protocol

Flow Cytometry

Use 10ul of the suggested working dilution to label 106 cells in 100ul.