Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Abelson murine leukemia virus-induced tumor

Age

adult

Gender

male

Strain

BALB/c

Applications

This cell line is a suitable transfection host.

The cells will pinocytose neutral red and will phagocytose latex beads and zymosan. They are capable of antibody dependent lysis of sheep erythrocytes and tumor cell targets. LPS or PPD treatment for 2 days stimulates lysis of erythrocytes but not tumor cell targets.

Storage Conditions

liquid nitrogen vapor phase

Images

Derivation

This line was established from a tumor induced by Abelson murine leukemia virus.

This cell line is easy to propagate, high efficiency for DNA transfection, sensitivity to RNA interference, and supports replication of murine noroviruses. This cell line is negative for surface immunoglobulin (sIg-), Ia (Ia-) and Thy-1.2 (Thy-1.2). When this line was established, it was described as not secreting detectable virus particles and negative using the XC plaque formation assay. Based on a published study by Dr. Janet W. Hartley in 2008, this line was demonstrated to express ecotropic and polytropic MuLV, and is positive using the XC plaque assay for virus replication.RefHartley JW, et al. Expression of infectious murine leukemia viruses by RAW264.7 cells, a potential complication for studies with a widely used mouse macrophage cell line. Retrovirology. 4: 5:1, 2008. PubMed 18177500.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Subcultures are prepared by scraping. For a 75 cm2 flask, remove all but 10 mL culture medium (adjust amount accordingly for other culture vessels). Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product. Dislodge cells from the flask substrate with a cell scraper; aspirate and add appropriate aliquots of the cell suspension into new culture vessels.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.