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class=\u0022elements-frag-data highwire-markup\u0022 id=\u0022fig-data\u0022\u003E\u003Cdiv id=\u0022fig-data-figures\u0022 class=\u0022group frag-figure\u0022\u003E\u003Cdiv class=\u0022fig-data-title-jump clearfix\u0022\u003E\u003Ch3 class=\u0022fig-data-group-title\u0022\u003EFigures\u003C\/h3\u003E\u003Cdiv class=\u0022fig-data-jump-links\u0022\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cdiv class=\u0022item-list\u0022\u003E\u003Cul class=\u0022fig-data-list clearfix\u0022 id=\u0022fragments-fig\u0022\u003E\u003Cli class=\u0022first\u0022\u003E\u003Cdiv class=\u0022element-fig-data clearfix figure-caption\u0022\u003E\u003Cdiv class=\u0022highwire-markup\u0022\u003E\u003Cdiv xmlns=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022 id=\u0022content-block-markup\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cdiv class=\u0022fig-expansion \u0022 id=\u0022F1\u0022\u003E\u003Cspan class=\u0022highwire-journal-article-marker-start\u0022\u003E\u003C\/span\u003E\u003Cdiv class=\u0022highwire-figure\u0022\u003E\u003Cdiv class=\u0022fig-inline-img-wrapper\u0022\u003E\u003Cdiv class=\u0022fig-inline-img\u0022\u003E\u003Ca href=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F1.large.jpg?width=800\u0026amp;height=600\u0026amp;carousel=1\u0022 title=\u0022A model of flavivirus maturation pathways. Immature particles form by budding into the endoplasmic reticulum. The envelopes of these particles have a single icosahedral symmetry. A conformational change of the virion occurs as the particles are transported to the acidic pH in the Golgi and TGN. The prM protein can be cleaved by the host protease furin after the conformational change, but not all prM molecules are cleaved. Depending on the fraction of prM cleaved, the particles might adopt mature, mosaic or immature\u0026#x2010;like conformations when released from cells. The cleaved precursor proteins are released from particles at neutral pH in the extracellular space. The immature\u0026#x2010;like particles only constitute approximately 3% of wild\u0026#x2010;type virions that are released from cells, but are abundant for the prM cleavage\u0026#x2010;deficient prR201A mutant. About 50% of the immature\u0026#x2010;like particles of the prR201A mutant had their envelopes organized into two regions, each of which correspond to an immature structure, but the two regions were symmetrically mismatched. The pr\/prM proteins are shown in red\/blue and the envelope proteins are shown in grey. The red and blue colours of prM indicate mismatched icosahedral symmetries. ER; endoplasmic reticulum; pr, precursor; prM, precursor membrane; TGN, trans\u0026#x2010;Golgi network.\u0022 class=\u0022highwire-fragment fragment-images colorbox-load\u0022 rel=\u0022gallery-fragment-images-988601124\u0022 data-figure-caption=\u0022\u0026lt;div class=\u0026quot;highwire-markup\u0026quot;\u0026gt;\u0026lt;div xmlns=\u0026quot;http:\/\/www.w3.org\/1999\/xhtml\u0026quot;\u0026gt;A model of flavivirus maturation pathways. Immature particles form by budding into the endoplasmic reticulum. The envelopes of these particles have a single icosahedral symmetry. A conformational change of the virion occurs as the particles are transported to the acidic pH in the Golgi and TGN. The prM protein can be cleaved by the host protease furin after the conformational change, but not all prM molecules are cleaved. Depending on the fraction of prM cleaved, the particles might adopt mature, mosaic or immature\u0026#x2010;like conformations when released from cells. The cleaved precursor proteins are released from particles at neutral pH in the extracellular space. The immature\u0026#x2010;like particles only constitute approximately 3% of wild\u0026#x2010;type virions that are released from cells, but are abundant for the prM cleavage\u0026#x2010;deficient prR201A mutant. About 50% of the immature\u0026#x2010;like particles of the prR201A mutant had their envelopes organized into two regions, each of which correspond to an immature structure, but the two regions were symmetrically mismatched. The pr\/prM proteins are shown in red\/blue and the envelope proteins are shown in grey. The red and blue colours of prM indicate mismatched icosahedral symmetries. ER; endoplasmic reticulum; pr, precursor; prM, precursor membrane; TGN, \u0026lt;em\u0026gt;trans\u0026lt;\/em\u0026gt;\u0026#x2010;Golgi network.\u0026lt;\/div\u0026gt;\u0026lt;\/div\u0026gt;\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003E\u003Cspan class=\u0022hw-responsive-img\u0022\u003E\u003Cimg class=\u0022highwire-fragment fragment-image lazyload\u0022 alt=\u0022Figure 1.\u0022 src=\u0022data:image\/gif;base64,R0lGODlhAQABAIAAAAAAAP\/\/\/yH5BAEAAAAALAAAAAABAAEAAAIBRAA7\u0022 data-src=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F1.medium.gif\u0022\/\u003E\u003Cnoscript\u003E\u003Cimg class=\u0022highwire-fragment fragment-image\u0022 alt=\u0022Figure 1.\u0022 src=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F1.medium.gif\u0022\/\u003E\u003C\/noscript\u003E\u003C\/span\u003E\u003C\/a\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cul class=\u0022highwire-figure-links inline\u0022\u003E\u003Cli class=\u0022download-fig first\u0022\u003E\u003Ca href=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F1.large.jpg?download=true\u0022 class=\u0022highwire-figure-link highwire-figure-link-download\u0022 title=\u0022Download Figure 1.\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload figure\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022new-tab\u0022\u003E\u003Ca href=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F1.large.jpg\u0022 class=\u0022highwire-figure-link highwire-figure-link-newtab\u0022 target=\u0022_blank\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EOpen in new tab\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022download-ppt last\u0022\u003E\u003Ca href=\u0022\/highwire\/powerpoint\/42366\u0022 class=\u0022highwire-figure-link highwire-figure-link-ppt\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload powerpoint\u003C\/a\u003E\u003C\/li\u003E\u003C\/ul\u003E\u003C\/div\u003E\u003Cdiv class=\u0022fig-caption\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cspan class=\u0022fig-label\u0022\u003EFigure 1.\u003C\/span\u003E \u003Cp id=\u0022p-5\u0022\u003EA model of flavivirus maturation pathways. Immature particles form by budding into the endoplasmic reticulum. The envelopes of these particles have a single icosahedral symmetry. A conformational change of the virion occurs as the particles are transported to the acidic pH in the Golgi and TGN. The prM protein can be cleaved by the host protease furin after the conformational change, but not all prM molecules are cleaved. Depending on the fraction of prM cleaved, the particles might adopt mature, mosaic or immature\u2010like conformations when released from cells. The cleaved precursor proteins are released from particles at neutral pH in the extracellular space. The immature\u2010like particles only constitute approximately 3% of wild\u2010type virions that are released from cells, but are abundant for the prM cleavage\u2010deficient prR201A mutant. About 50% of the immature\u2010like particles of the prR201A mutant had their envelopes organized into two regions, each of which correspond to an immature structure, but the two regions were symmetrically mismatched. The pr\/prM proteins are shown in red\/blue and the envelope proteins are shown in grey. The red and blue colours of prM indicate mismatched icosahedral symmetries. ER; endoplasmic reticulum; pr, precursor; prM, precursor membrane; TGN, \u003Cem\u003Etrans\u003C\/em\u003E\u2010Golgi network.\u003C\/p\u003E\u003Cdiv class=\u0022sb-div caption-clear\u0022\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cspan class=\u0022highwire-journal-article-marker-end\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003Cspan id=\u0022related-urls\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/li\u003E\u003Cli\u003E\u003Cdiv class=\u0022element-fig-data clearfix figure-caption\u0022\u003E\u003Cdiv class=\u0022highwire-markup\u0022\u003E\u003Cdiv xmlns=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022 id=\u0022content-block-markup\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cdiv class=\u0022fig-expansion \u0022 id=\u0022F2\u0022\u003E\u003Cspan class=\u0022highwire-journal-article-marker-start\u0022\u003E\u003C\/span\u003E\u003Cdiv class=\u0022highwire-figure\u0022\u003E\u003Cdiv class=\u0022fig-inline-img-wrapper\u0022\u003E\u003Cdiv class=\u0022fig-inline-img\u0022\u003E\u003Ca href=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F2.large.jpg?width=800\u0026amp;height=600\u0026amp;carousel=1\u0022 title=\u0022Central section through a tomogram of particles produced by wild\u0026#x2010;type DENV2 infection. Mature, mosaic and immature\u0026#x2010;like particles are indicated by blue, red and green arrows, respectively. DENV2, dengue virus type 2.\u0022 class=\u0022highwire-fragment fragment-images colorbox-load\u0022 rel=\u0022gallery-fragment-images-988601124\u0022 data-figure-caption=\u0022\u0026lt;div class=\u0026quot;highwire-markup\u0026quot;\u0026gt;Central section through a tomogram of particles produced by wild\u0026#x2010;type DENV2 infection. Mature, mosaic and immature\u0026#x2010;like particles are indicated by blue, red and green arrows, respectively. DENV2, dengue virus type 2.\u0026lt;\/div\u0026gt;\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003E\u003Cspan class=\u0022hw-responsive-img\u0022\u003E\u003Cimg class=\u0022highwire-fragment fragment-image lazyload\u0022 alt=\u0022Figure 2.\u0022 src=\u0022data:image\/gif;base64,R0lGODlhAQABAIAAAAAAAP\/\/\/yH5BAEAAAAALAAAAAABAAEAAAIBRAA7\u0022 data-src=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F2.medium.gif\u0022\/\u003E\u003Cnoscript\u003E\u003Cimg class=\u0022highwire-fragment fragment-image\u0022 alt=\u0022Figure 2.\u0022 src=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F2.medium.gif\u0022\/\u003E\u003C\/noscript\u003E\u003C\/span\u003E\u003C\/a\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cul class=\u0022highwire-figure-links inline\u0022\u003E\u003Cli class=\u0022download-fig first\u0022\u003E\u003Ca href=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F2.large.jpg?download=true\u0022 class=\u0022highwire-figure-link highwire-figure-link-download\u0022 title=\u0022Download Figure 2.\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload figure\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022new-tab\u0022\u003E\u003Ca href=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F2.large.jpg\u0022 class=\u0022highwire-figure-link highwire-figure-link-newtab\u0022 target=\u0022_blank\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EOpen in new tab\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022download-ppt last\u0022\u003E\u003Ca href=\u0022\/highwire\/powerpoint\/42450\u0022 class=\u0022highwire-figure-link highwire-figure-link-ppt\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload powerpoint\u003C\/a\u003E\u003C\/li\u003E\u003C\/ul\u003E\u003C\/div\u003E\u003Cdiv class=\u0022fig-caption\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cspan class=\u0022fig-label\u0022\u003EFigure 2.\u003C\/span\u003E \u003Cp id=\u0022p-9\u0022\u003ECentral section through a tomogram of particles produced by wild\u2010type DENV2 infection. Mature, mosaic and immature\u2010like particles are indicated by blue, red and green arrows, respectively. DENV2, dengue virus type 2.\u003C\/p\u003E\u003Cdiv class=\u0022sb-div caption-clear\u0022\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cspan class=\u0022highwire-journal-article-marker-end\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003Cspan id=\u0022related-urls\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/li\u003E\u003Cli\u003E\u003Cdiv class=\u0022element-fig-data clearfix figure-caption\u0022\u003E\u003Cdiv class=\u0022highwire-markup\u0022\u003E\u003Cdiv xmlns=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022 id=\u0022content-block-markup\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cdiv class=\u0022fig-expansion \u0022 id=\u0022F3\u0022\u003E\u003Cspan class=\u0022highwire-journal-article-marker-start\u0022\u003E\u003C\/span\u003E\u003Cdiv class=\u0022highwire-figure\u0022\u003E\u003Cdiv class=\u0022fig-inline-img-wrapper\u0022\u003E\u003Cdiv class=\u0022fig-inline-img\u0022\u003E\u003Ca href=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F3.large.jpg?width=800\u0026amp;height=600\u0026amp;carousel=1\u0022 title=\u0022Cross\u0026#x2010;correlation searches to determine the orientation of particles in the sub\u0026#x2010;tomograms. Model particles in different orientations were compared with particles in the sub\u0026#x2010;tomograms and the resulting correlation coefficients were plotted on a stereographic projection (the plots are analogous to the representation of the surface of the Earth onto a flat map). See Methods section for details. (A) Comparison of the wild\u0026#x2010;type mosaic particle sub\u0026#x2010;tomogram with the immature dengue virus structure. (B) Comparison of the prR201A mutant immature\u0026#x2010;like particle with the immature dengue virus structure. Peaks in the searches correspond to positions of fivefold symmetry axes in the tomograms (see Methods section for details). The interval between contours is 1\u0026#x3C3;. The coloured lines connect the icosahedral fivefold symmetry axes. The search in (B) shows peaks corresponding to two independent icosahedral symmetries.\u0022 class=\u0022highwire-fragment fragment-images colorbox-load\u0022 rel=\u0022gallery-fragment-images-988601124\u0022 data-figure-caption=\u0022\u0026lt;div class=\u0026quot;highwire-markup\u0026quot;\u0026gt;\u0026lt;div xmlns=\u0026quot;http:\/\/www.w3.org\/1999\/xhtml\u0026quot;\u0026gt;Cross\u0026#x2010;correlation searches to determine the orientation of particles in the sub\u0026#x2010;tomograms. Model particles in different orientations were compared with particles in the sub\u0026#x2010;tomograms and the resulting correlation coefficients were plotted on a stereographic projection (the plots are analogous to the representation of the surface of the Earth onto a flat map). See Methods section for details. (\u0026lt;strong\u0026gt;A\u0026lt;\/strong\u0026gt;) Comparison of the wild\u0026#x2010;type mosaic particle sub\u0026#x2010;tomogram with the immature dengue virus structure. (\u0026lt;strong\u0026gt;B\u0026lt;\/strong\u0026gt;) Comparison of the prR201A mutant immature\u0026#x2010;like particle with the immature dengue virus structure. Peaks in the searches correspond to positions of fivefold symmetry axes in the tomograms (see Methods section for details). The interval between contours is 1\u0026#x3C3;. The coloured lines connect the icosahedral fivefold symmetry axes. The search in (\u0026lt;strong\u0026gt;B\u0026lt;\/strong\u0026gt;) shows peaks corresponding to two independent icosahedral symmetries.\u0026lt;\/div\u0026gt;\u0026lt;\/div\u0026gt;\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003E\u003Cspan class=\u0022hw-responsive-img\u0022\u003E\u003Cimg class=\u0022highwire-fragment fragment-image lazyload\u0022 alt=\u0022Figure 3.\u0022 src=\u0022data:image\/gif;base64,R0lGODlhAQABAIAAAAAAAP\/\/\/yH5BAEAAAAALAAAAAABAAEAAAIBRAA7\u0022 data-src=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F3.medium.gif\u0022\/\u003E\u003Cnoscript\u003E\u003Cimg class=\u0022highwire-fragment fragment-image\u0022 alt=\u0022Figure 3.\u0022 src=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F3.medium.gif\u0022\/\u003E\u003C\/noscript\u003E\u003C\/span\u003E\u003C\/a\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cul class=\u0022highwire-figure-links inline\u0022\u003E\u003Cli class=\u0022download-fig first\u0022\u003E\u003Ca href=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F3.large.jpg?download=true\u0022 class=\u0022highwire-figure-link highwire-figure-link-download\u0022 title=\u0022Download Figure 3.\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload figure\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022new-tab\u0022\u003E\u003Ca href=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F3.large.jpg\u0022 class=\u0022highwire-figure-link highwire-figure-link-newtab\u0022 target=\u0022_blank\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EOpen in new tab\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022download-ppt last\u0022\u003E\u003Ca href=\u0022\/highwire\/powerpoint\/42452\u0022 class=\u0022highwire-figure-link highwire-figure-link-ppt\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload powerpoint\u003C\/a\u003E\u003C\/li\u003E\u003C\/ul\u003E\u003C\/div\u003E\u003Cdiv class=\u0022fig-caption\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cspan class=\u0022fig-label\u0022\u003EFigure 3.\u003C\/span\u003E \u003Cp id=\u0022p-10\u0022\u003ECross\u2010correlation searches to determine the orientation of particles in the sub\u2010tomograms. Model particles in different orientations were compared with particles in the sub\u2010tomograms and the resulting correlation coefficients were plotted on a stereographic projection (the plots are analogous to the representation of the surface of the Earth onto a flat map). See Methods section for details. (\u003Cstrong\u003EA\u003C\/strong\u003E) Comparison of the wild\u2010type mosaic particle sub\u2010tomogram with the immature dengue virus structure. (\u003Cstrong\u003EB\u003C\/strong\u003E) Comparison of the prR201A mutant immature\u2010like particle with the immature dengue virus structure. Peaks in the searches correspond to positions of fivefold symmetry axes in the tomograms (see Methods section for details). The interval between contours is 1\u03c3. The coloured lines connect the icosahedral fivefold symmetry axes. The search in (\u003Cstrong\u003EB\u003C\/strong\u003E) shows peaks corresponding to two independent icosahedral symmetries.\u003C\/p\u003E\u003Cdiv class=\u0022sb-div caption-clear\u0022\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cspan class=\u0022highwire-journal-article-marker-end\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003Cspan id=\u0022related-urls\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/li\u003E\u003Cli\u003E\u003Cdiv class=\u0022element-fig-data clearfix figure-caption\u0022\u003E\u003Cdiv class=\u0022highwire-markup\u0022\u003E\u003Cdiv xmlns=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022 id=\u0022content-block-markup\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cdiv class=\u0022fig-expansion \u0022 id=\u0022F4\u0022\u003E\u003Cspan class=\u0022highwire-journal-article-marker-start\u0022\u003E\u003C\/span\u003E\u003Cdiv class=\u0022highwire-figure\u0022\u003E\u003Cdiv class=\u0022fig-inline-img-wrapper\u0022\u003E\u003Cdiv class=\u0022fig-inline-img\u0022\u003E\u003Ca href=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F4.large.jpg?width=800\u0026amp;height=600\u0026amp;carousel=1\u0022 title=\u0022Structural analysis of a dengue virus type 2 mosaic particle. (A) The colours of the icosahedral faces represent the correlation coefficient for a particular icosahedral asymmetrical unit, compared with immature (upper row) and mature (bottom row) dengue virus structures. The icosahedral symmetry of the mature part of the particle is shown to be coincident with the symmetry of the immature part, but this could not be experimentally verified. (B) Model of the mosaic particle. Regions that had higher than 0.25 correlation with the immature structure are shown as an immature structure in blue. Regions that had higher than 0.25 correlations with the mature structure are shown as a spherical orange surface.\u0022 class=\u0022highwire-fragment fragment-images colorbox-load\u0022 rel=\u0022gallery-fragment-images-988601124\u0022 data-figure-caption=\u0022\u0026lt;div class=\u0026quot;highwire-markup\u0026quot;\u0026gt;\u0026lt;div xmlns=\u0026quot;http:\/\/www.w3.org\/1999\/xhtml\u0026quot;\u0026gt;Structural analysis of a dengue virus type 2 mosaic particle. (\u0026lt;strong\u0026gt;A\u0026lt;\/strong\u0026gt;) The colours of the icosahedral faces represent the correlation coefficient for a particular icosahedral asymmetrical unit, compared with immature (upper row) and mature (bottom row) dengue virus structures. The icosahedral symmetry of the mature part of the particle is shown to be coincident with the symmetry of the immature part, but this could not be experimentally verified. (\u0026lt;strong\u0026gt;B\u0026lt;\/strong\u0026gt;) Model of the mosaic particle. Regions that had higher than 0.25 correlation with the immature structure are shown as an immature structure in blue. Regions that had higher than 0.25 correlations with the mature structure are shown as a spherical orange surface.\u0026lt;\/div\u0026gt;\u0026lt;\/div\u0026gt;\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003E\u003Cspan class=\u0022hw-responsive-img\u0022\u003E\u003Cimg class=\u0022highwire-fragment fragment-image lazyload\u0022 alt=\u0022Figure 4.\u0022 src=\u0022data:image\/gif;base64,R0lGODlhAQABAIAAAAAAAP\/\/\/yH5BAEAAAAALAAAAAABAAEAAAIBRAA7\u0022 data-src=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F4.medium.gif\u0022\/\u003E\u003Cnoscript\u003E\u003Cimg class=\u0022highwire-fragment fragment-image\u0022 alt=\u0022Figure 4.\u0022 src=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F4.medium.gif\u0022\/\u003E\u003C\/noscript\u003E\u003C\/span\u003E\u003C\/a\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cul class=\u0022highwire-figure-links inline\u0022\u003E\u003Cli class=\u0022download-fig first\u0022\u003E\u003Ca href=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F4.large.jpg?download=true\u0022 class=\u0022highwire-figure-link highwire-figure-link-download\u0022 title=\u0022Download Figure 4.\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload figure\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022new-tab\u0022\u003E\u003Ca href=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F4.large.jpg\u0022 class=\u0022highwire-figure-link highwire-figure-link-newtab\u0022 target=\u0022_blank\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EOpen in new tab\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022download-ppt last\u0022\u003E\u003Ca href=\u0022\/highwire\/powerpoint\/42453\u0022 class=\u0022highwire-figure-link highwire-figure-link-ppt\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload powerpoint\u003C\/a\u003E\u003C\/li\u003E\u003C\/ul\u003E\u003C\/div\u003E\u003Cdiv class=\u0022fig-caption\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cspan class=\u0022fig-label\u0022\u003EFigure 4.\u003C\/span\u003E \u003Cp id=\u0022p-12\u0022\u003EStructural analysis of a dengue virus type 2 mosaic particle. (\u003Cstrong\u003EA\u003C\/strong\u003E) The colours of the icosahedral faces represent the correlation coefficient for a particular icosahedral asymmetrical unit, compared with immature (upper row) and mature (bottom row) dengue virus structures. The icosahedral symmetry of the mature part of the particle is shown to be coincident with the symmetry of the immature part, but this could not be experimentally verified. (\u003Cstrong\u003EB\u003C\/strong\u003E) Model of the mosaic particle. Regions that had higher than 0.25 correlation with the immature structure are shown as an immature structure in blue. Regions that had higher than 0.25 correlations with the mature structure are shown as a spherical orange surface.\u003C\/p\u003E\u003Cdiv class=\u0022sb-div caption-clear\u0022\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cspan class=\u0022highwire-journal-article-marker-end\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003Cspan id=\u0022related-urls\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/li\u003E\u003Cli class=\u0022last\u0022\u003E\u003Cdiv class=\u0022element-fig-data clearfix figure-caption\u0022\u003E\u003Cdiv class=\u0022highwire-markup\u0022\u003E\u003Cdiv xmlns=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022 id=\u0022content-block-markup\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cdiv class=\u0022fig-expansion \u0022 id=\u0022F5\u0022\u003E\u003Cspan class=\u0022highwire-journal-article-marker-start\u0022\u003E\u003C\/span\u003E\u003Cdiv class=\u0022highwire-figure\u0022\u003E\u003Cdiv class=\u0022fig-inline-img-wrapper\u0022\u003E\u003Cdiv class=\u0022fig-inline-img\u0022\u003E\u003Ca href=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F5.large.jpg?width=800\u0026amp;height=600\u0026amp;carousel=1\u0022 title=\u0022Structural analysis of the dengue virus type 2 prR201A mutant immature\u0026#x2010;like particle. (A) The colours of the icosahedral faces represent the correlation coefficient for a particular icosahedral asymmetrical unit compared with the immature dengue virus structure in one of the two alternative orientations. The orientations of icosahedrons in the top and bottom rows show the relative orientations of the icosahedral symmetries of the two regions. (B) Model of the immature particle with double symmetry. Regions that had higher than 0.25 correlation with the immature structure in orientation 1 are shown in blue. Similarly, regions that had higher than 0.25 correlations with the immature structure in orientation 2 are shown in orange.\u0022 class=\u0022highwire-fragment fragment-images colorbox-load\u0022 rel=\u0022gallery-fragment-images-988601124\u0022 data-figure-caption=\u0022\u0026lt;div class=\u0026quot;highwire-markup\u0026quot;\u0026gt;\u0026lt;div xmlns=\u0026quot;http:\/\/www.w3.org\/1999\/xhtml\u0026quot;\u0026gt;Structural analysis of the dengue virus type 2 prR201A mutant immature\u0026#x2010;like particle. (\u0026lt;strong\u0026gt;A\u0026lt;\/strong\u0026gt;) The colours of the icosahedral faces represent the correlation coefficient for a particular icosahedral asymmetrical unit compared with the immature dengue virus structure in one of the two alternative orientations. The orientations of icosahedrons in the top and bottom rows show the relative orientations of the icosahedral symmetries of the two regions. (\u0026lt;strong\u0026gt;B\u0026lt;\/strong\u0026gt;) Model of the immature particle with double symmetry. Regions that had higher than 0.25 correlation with the immature structure in orientation 1 are shown in blue. Similarly, regions that had higher than 0.25 correlations with the immature structure in orientation 2 are shown in orange.\u0026lt;\/div\u0026gt;\u0026lt;\/div\u0026gt;\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003E\u003Cspan class=\u0022hw-responsive-img\u0022\u003E\u003Cimg class=\u0022highwire-fragment fragment-image lazyload\u0022 alt=\u0022Figure 5.\u0022 src=\u0022data:image\/gif;base64,R0lGODlhAQABAIAAAAAAAP\/\/\/yH5BAEAAAAALAAAAAABAAEAAAIBRAA7\u0022 data-src=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F5.medium.gif\u0022\/\u003E\u003Cnoscript\u003E\u003Cimg class=\u0022highwire-fragment fragment-image\u0022 alt=\u0022Figure 5.\u0022 src=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F5.medium.gif\u0022\/\u003E\u003C\/noscript\u003E\u003C\/span\u003E\u003C\/a\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cul class=\u0022highwire-figure-links inline\u0022\u003E\u003Cli class=\u0022download-fig first\u0022\u003E\u003Ca href=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F5.large.jpg?download=true\u0022 class=\u0022highwire-figure-link highwire-figure-link-download\u0022 title=\u0022Download Figure 5.\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload figure\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022new-tab\u0022\u003E\u003Ca href=\u0022http:\/\/embor.embopress.org\/content\/embor\/12\/6\/602\/F5.large.jpg\u0022 class=\u0022highwire-figure-link highwire-figure-link-newtab\u0022 target=\u0022_blank\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EOpen in new tab\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022download-ppt last\u0022\u003E\u003Ca href=\u0022\/highwire\/powerpoint\/42455\u0022 class=\u0022highwire-figure-link highwire-figure-link-ppt\u0022 data-icon-position=\u0022\u0022 data-hide-link-title=\u00220\u0022\u003EDownload powerpoint\u003C\/a\u003E\u003C\/li\u003E\u003C\/ul\u003E\u003C\/div\u003E\u003Cdiv class=\u0022fig-caption\u0022 xmlns:xhtml=\u0022http:\/\/www.w3.org\/1999\/xhtml\u0022\u003E\u003Cspan class=\u0022fig-label\u0022\u003EFigure 5.\u003C\/span\u003E \u003Cp id=\u0022p-14\u0022\u003EStructural analysis of the dengue virus type 2 prR201A mutant immature\u2010like particle. (\u003Cstrong\u003EA\u003C\/strong\u003E) The colours of the icosahedral faces represent the correlation coefficient for a particular icosahedral asymmetrical unit compared with the immature dengue virus structure in one of the two alternative orientations. The orientations of icosahedrons in the top and bottom rows show the relative orientations of the icosahedral symmetries of the two regions. (\u003Cstrong\u003EB\u003C\/strong\u003E) Model of the immature particle with double symmetry. Regions that had higher than 0.25 correlation with the immature structure in orientation 1 are shown in blue. Similarly, regions that had higher than 0.25 correlations with the immature structure in orientation 2 are shown in orange.\u003C\/p\u003E\u003Cdiv class=\u0022sb-div caption-clear\u0022\u003E\u003C\/div\u003E\u003C\/div\u003E\u003Cspan class=\u0022highwire-journal-article-marker-end\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003Cspan id=\u0022related-urls\u0022\u003E\u003C\/span\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/li\u003E\u003C\/ul\u003E\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E \u003C\/div\u003E\n\n \n \u003C\/div\u003E\n\u003C\/div\u003E\n \u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\u003Cscript type=\u0022text\/javascript\u0022 defer=\u0022defer\u0022 src=\u0022http:\/\/embor.embopress.org\/sites\/all\/libraries\/lazysizes\/lazysizes.min.js?pcsxuv\u0022\u003E\u003C\/script\u003E\n\u003Cscript type=\u0022text\/javascript\u0022 src=\u0022http:\/\/embor.embopress.org\/sites\/all\/modules\/highwire\/highwire\/plugins\/highwire_markup_process\/js\/highwire_figures.js?pcsxuv\u0022\u003E\u003C\/script\u003E\n\u003C\/body\u003E\u003C\/html\u003E"}