[Histonet] exposing epitope region on MCL of rat

From:

"Andrew Lust"

Dear Histonetters
I have a quick question, I was wondering if anybody has worked with Medial
collateral ligament of the rat in immuno-fluoresces staining? If you have,
did you have to do any type of epitope exposure to the tissue?
I know companies offer pre made solutions, but have no clue if I need to use
this solution. Considering that I am not going there paraffin embedded
tissue, so I am not exposing the tissue to harsh conditions that would
degrade proteins on my tissue.
Staining so far has been questionable, so I am up for any suggestions on
fluoresce staining on the MCL of the Rat.
thanks again
Andrew L
lab tech
University of Houston
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