イベント情報

【GIR公開セミナー】Dr. Koji Sode/Dr. Man Bock Gu

13:00 – 13:40
Plenary lecture 1
“Science and Engineering of Biomolecules Capable of Direct Electron Transfer
-Expectation and Future of DET based Biomedical Engineering- ”
Prof. Koji Sode, William R. Kenan Jr. Distinguished Professor
The Joint Department of Biomedical Engineering, The University of North Carolina at Chapel Hill and North Carolina State University

[要旨]
Direct electron transfer (DET) reactions between biomolecules and transducers are the most intriguing technology in the current bioelectronics. Historically, DET principle is recognized as the electron transfer from enzyme active site to electrode directly in the absence of any additional electron acceptors, or mediators. This reaction is useful for various biodevices such as biosensors and biofuel-cells, especially by focusing their potential application in in vivo, continuous monitoring and operation. However, the numbers of enzymes/proteins and biomolecules inherently capable of DET is limited. We have been studying in the search, engineering and creation of DET biomolecules and their applications to construct biomedical devices based on varieties of electrochemical principles, e.g. amperometric, potentiometric, impedometric . In this presentation, I will introduce the current understanding and challenge in creating of biomolecule capable of DET, and address the expectation and the future of DET based biomedical engineering.

14:00 – 14:20
“Development of a fluorogenic G-quadruplex ligand based upon its conformational switching”
Assoc. Prof. Masayuki Tera, Department of Life science and biotechnology, Tokyo University of Agriculture and Technology

[要旨]
Aptamers, small sizes of ssDNA or RNA, have been well known as bioreceptor molecules with affinity and specificity over a certain target since 1990. One of the tedious and sometime serious hurdles to develop aptamers is to immobilize the target moieties to the solid matrixes mainly for the separation of unbound library from bound ones. There have been a lot of efforts to elaborate many special methods to develop aptamers without target immobilization. We have also successfully developed an immobilization-free screening of aptamers by using graphene-oxide (GO-SELEX) for the first time in world and this method has been widely and successfully used not only in my own lab and world-widely. Interestingly, we have found out that this method is specifically useful for finding a pair of aptamers binding at different binding sites of the same target moiety simultaneously, which will definitely lead to the sandwich-type binding of aptamer 1-target-aptamer 2. In this presentation, it will be explained how we conducted this GO-SELEX for a few different targets, including disease biomarker proteins and pandemic avian influenza viruses successfully, and how the different biosensing platforms, including electrochemical biosensing system employing a mini-potentiostat, can be realized on a few different nano/micro-sized surfaces of organic/inorganic materials.