Graphical Abstract:

Abstract:

Background: Laccase, a multicopper oxidoreductase (EC: 1.10.3.2), is a widely used
enzyme in bioremediation of textile dye effluents. Fungal Laccase is preferably used as a remediating
agent in the treatment and transformation of toxic organic pollutants. In this study, crude
laccase from a basidiomycetes fungus, Phanerochaete sordida, was able to decolorize azo, antroquinone
and indigoid dyes. In addition, interactions between dyes and enzyme were analysed using
molecular docking studies.

Methods: In this work, a white rot basidiomycete’s fungus, Phanerochaete sordida, was selected
from forest soil isolates of Eastern Ghats, and Tirumala and lignolytic enzymes production was
assayed after 7 days of incubation. The crude enzyme was checked for decolourisation of various
synthetic textile dyes (Vat Brown, Acid Blue, Indigo, Reactive Blue and Reactive Black). Molecular
docking studies were done using Autodock-4.2 to understand the interactions between dyes and
enzymes.

Results: Highest decolourisation efficiency was achieved with the crude enzyme in case of vat
brown whereas the lowest decolourisation efficiency was achieved in Reactive blue decolourisation.
Similar results were observed in their binding affinity with lignin peroxidase of Phanerochaete
chrysosporium through molecular docking approach.

Conclusion: Thus, experimental results and subsequent in silico validation involving an advanced
remediation approach would be useful to reduce time and cost in other similar experiments.

Background: Laccase, a multicopper oxidoreductase (EC: 1.10.3.2), is a widely used
enzyme in bioremediation of textile dye effluents. Fungal Laccase is preferably used as a remediating
agent in the treatment and transformation of toxic organic pollutants. In this study, crude
laccase from a basidiomycetes fungus, Phanerochaete sordida, was able to decolorize azo, antroquinone
and indigoid dyes. In addition, interactions between dyes and enzyme were analysed using
molecular docking studies.

Methods: In this work, a white rot basidiomycete’s fungus, Phanerochaete sordida, was selected
from forest soil isolates of Eastern Ghats, and Tirumala and lignolytic enzymes production was
assayed after 7 days of incubation. The crude enzyme was checked for decolourisation of various
synthetic textile dyes (Vat Brown, Acid Blue, Indigo, Reactive Blue and Reactive Black). Molecular
docking studies were done using Autodock-4.2 to understand the interactions between dyes and
enzymes.

Results: Highest decolourisation efficiency was achieved with the crude enzyme in case of vat
brown whereas the lowest decolourisation efficiency was achieved in Reactive blue decolourisation.
Similar results were observed in their binding affinity with lignin peroxidase of Phanerochaete
chrysosporium through molecular docking approach.

Conclusion: Thus, experimental results and subsequent in silico validation involving an advanced
remediation approach would be useful to reduce time and cost in other similar experiments.