↵a HeLa P4 cells were cotransfected in six-well trays with pCDNA3 expression vectors for different MYC-tagged forms of US28 and with EGFP-N1, a GFP expression vector (6:1 ratio). Cells were harvested 36 h later, stained with the anti-MYC monoclonal antibody 9E10 and with a PE-conjugated secondary antibody, and analyzed for GFP expression (green fluorescence) and MYC expression (red fluorescence). Results are from the same series of transfections.