Application Information: sRANKL antibody [IK22/5]

BL: Use at an assay dependent dilution. The Low Endotoxin Azide Free format is suggested for blocking studies. FACS: Use 0.5μg for 10^6 cells 100μl volume. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 35 kDa. Optimal dilutions/concentrations should be determined by the end user.

Form Supplied

Liquid

Concentration

0.5
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Protein A purified

Purification Note

The antibody was purified by affinity chromatography.

Specifications: sRANKL antibody [IK22/5]

Full Name

tumor necrosis factor (ligand) superfamily, member 11

Product Description

Rat monoclonal [IK22/5] to sRANKL

Background

sRANKL is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. sRANKL deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis

Synonyms

RANKL, Tnfsf11, Ly109l, sRANKL, ODF, OPGL, 21943, Trance, OPG

Cellular Localization

Cell Membrane and Secreted.

Specificity

This antibody reacts with sRANKL.

Host

Rat

Clonality

Monoclonal

Clone Name

IK22/5

Target

sRANKL

Isotype

IgG2a

Immunogen

NSO derived recombinant mouse TRANCE.

Light Chain

kappa

Antigen Species

Mouse

Species Reactivity

Mouse

Storage Conditions: sRANKL antibody [IK22/5]

Storage Buffer

Preservative: 0.09% Sodium Azide. Constituents: PBS. pH 7.2

Storage Instruction

Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Notes

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.