Dinosaurs’ loss was frogs’ gain

Most of the frogs alive today owe a big thank you to the asteroid or comet that delivered the coup de grace to the dinosaurs.

The frog Hyla sanchiangensis from eastern China is a descendant of one of three lineages (Hyloidea) that made it through Earth’s last mass extinction 66 million years ago to flourish worldwide today. Its ancestors diversified out of South America. (Image courtesy of Peng Zhang, Sun Yat-Sen University)

A new study by Chinese and American biologists shows that if the calamity had not wiped the planet clean of most terrestrial life 66 million years ago, 88 percent of today’s frog species wouldn’t be here. Nearly nine out of 10 species of frog today have descended from just three lineages that survived the mass extinction.

The results, published online today in the journal Proceedings of the National Academy of Sciences, are a surprise, because previous studies of frog evolution pinpointed the blossoming of the main frog lineages today to about 35 million years earlier, in the middle of the Mesozoic era.

The new analysis of 95 genes from frogs within 44 of 55 living families shows that these three lineages started to take off precisely at the boundary between the Cretaceous and Paleogene periods – the K-Pg boundary, formerly called the KT boundary – when the last mass extinction occurred, and not 100 million years ago.

According to herpetologist and co-author David Wake, a University of California, Berkeley professor of the graduate school and a curator of the Museum of Vertebrate Zoology, new frog species likely radiated rapidly throughout the world because so many environmental niches were available after the animals occupying them disappeared.

“We think the world was quite impoverished as a result of the KT event, and when the vegetation came back, angiosperms dominated. That’s when trees evolved to their full flowering,” Wake said. “Frogs started becoming arboreal. It was the arboreality that led to the great radiation in South America in particular.”

Taking to the trees

Trees are an ideal habitat for frogs not only because they allow them to escape from terrestrial predators, but also because their fallen leaves provide protection while the frogs are on the ground, breeding habitat and plenty of food, such as insects. Trees and other flowering plants took off in the late Cretaceous, and were ready for exploitation by frogs after they recovered from the extinction.

The frog Chalcorana raniceps from Sarawak on Borneo is a member of the Ranoidea, one of three successful surviving frog lineages. Its ancestors diversified out of Africa. (Yu Zeng photo)

Another adaptation that became popular was direct development, that is, producing young without a tadpole stage, which is standard for about half of all frog species today.

“The majority of the frogs that thrive now are thriving because of direct development of eggs in terrestrial situations,” he said. “It is a combination of direct development and use of arboreal habitat that accounts for a great deal of the radiation.”

Previous genetic analyses of frog evolution focused on mitochondrial DNA and how long the molecular clock had been ticking for mitochrondrial genes. However, analysis of molecular evolution in mitochondrial DNA often produces dates for lineage divergence that are too old. In the case of frogs, such analysis pinpointed the radiation of most living frogs at about 100 million years ago, which was a puzzle, since Earth’s environment was stable at that time. A changing environment typically drives evolution.

The new analysis, based on data assembled primarily by graduate student Yan-Jie Feng at Sun Yat-Sen University in Guangzhou, China, focused on the sequences of 95 genes located on chromosomes in the nucleus and how they changed over time. He and his colleagues gathered genetic data from 156 frog species and combined this with earlier information about two genes from 145 different frogs, for a total of 301 distinct frog species from all 55 families of frogs. The data were calibrated using 20 dates derived from fossils and Earth historical events.

The team, which includes scientists from the Florida Museum of Natural History at the University of Florida and the University of Texas, Austin, concluded that perhaps 10 groups of frogs survived the extinction, but only three of them (Hyloidea, Microhylidae, and Natatanura) flourished and diversified to claim habitats and niches around the world.

The role of luck in mass extinctions

The frog Scaphiophryne marmorata, or green burrowing frog, is endemic to Madagascar and a member of the family Microhylidae, one of three frog lineages that rapidly diversified after Cretaceous extinction ~66 million years ago. Brian Freiermuth photo.

Nothing other than luck distinguishes the survivors, Wake said. Remnants of the other surviving lineages are scattered in isolated spots around the world, but are just as diverse today in their habitats and breeding strategies as the 88 percent.

Two of the three surviving lineages that subsequently radiated widely came out of Africa, which remained intact as the continents shifted around over the ensuing eons, with the breakup of Pangea and then Gondwana to form the continents we see today. The African rift zone and mountain building in West Africa generated new habitats for the evolving frogs, Wake noted. The third, Hyloidea, radiated throughout what became South America.

Today’s frogs, comprising more than 6,700 known species, as well as many other animal and plant species are under severe stress around the world because of habitat destruction, human population explosion and climate change, possibly heralding a new period of mass extinction. The new study provides one clear message for future generations.

“These frogs made it through on luck, perhaps because they were either underground or could stay underground for long periods of time,” Wake said. “This certainly draws renewed attention to the positive aspects of mass extinctions: They provide ecological opportunity for new things. Just wait for the next grand extinction and life will take off again. In which direction it will take off, you don’t know.”

The paper’s other co-authors are David Cannatella and David Hillis at UT Austin, Peng Zhang and Dan Liang of Sun Yat-Sen and David Blackburn of the Florida Museum. Cannatella, Zhang and Liang are all former UC Berkeley postdoctoral fellows.

Support for the research was provided by the U.S. National Science Foundation, the National Natural Science Foundation of China and the National Youth Talent Support Program and National Science Fund for Excellent Young Scholars of China.
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