Prey Rescue by Colony PCR

Matchmaker Insert Check PCR Mix 2 is a 2X premix that allows quick and easy PCR amplification directly from yeast colonies. It is a complete mix designed to be used with our Matchmaker Gold Yeast One- and Two-Hybrid Screening Systems to quickly amplify, sort, and analyze the cDNA inserts in prey/library plasmids from positive clones identified in library screens. The mix is compatible with GAL4 AD libraries constructed in the following vectors: pGADT7, pGADT7-Rec, pGADT7-Rec2, pGAD424, pACT, pACT2, pGAD GH, pGAD GL, and pGAD10.

The Insert Check PCR Mix 2 is a complete mix. However, if you wish to purchase the primers separately, our Matchmaker AD LD-Insert Screening Amplimer Set, when combined with Advantage 2 PCR polymerase, enables you to amplify inserts from any of our Matchmaker GAL4 activation domain (AD) vectors. The 5' primers can also be used as sequencing primers.

Features

Applications

Yeast two-hybrid screening

Yeast one-hybrid screening

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.