Superresolution with Microscope Systems from Carl Zeiss

With ELYRA, Carl Zeiss
is introducing a product family that allows the superresolution PAL-M and SR-SIM
microscopy techniques to be comprehensively used and individually combined for
the very first time. This enables scientists in biomedical research to image
cellular structures marked with fluorescent dyes with maximum spatial resolution
below the classic diffraction limit of microscopes, i.e. under 200 nanometers.
This offers new possibilities for the analysis of the spatial relationship between
the smallest cellular components down to the individual molecule.

With the PAL-M and SR-SIM microscopy techniques, the ELYRA microscope system offers superresolution beyond the classic diffraction limit of microscopes.

The system line comprises three microscope systems: ELYRA S.1 (SR-SIM) is the
first microscope system to allow the use of SR-SIM technology on the basis of
a standard microscope stand. With ELYRA P.1, PAL-M technology is now being offered
commercially for the first time. The ELYRA PS.1 combined system permits the
very first combination of the two technologies on the same stand, both with
each other and with a laser scanning microscope. This means that an object can
be imaged with the three LSM, SR-SIM and PAL-M techniques in succession.

ELYRA gives users the opportunity to acquire the superresolution systems on
either a “turnkey” or modular basis. They no longer need to invest
in single extremely specialized and expensive systems. PAL-M technology offers
the highest resolution currently available while the outstanding feature of
SR-SIM technology is its high level of flexibility in the choice of dye. This
means that both superresolution methods are available without the previous limitations
in dye selection or user-friendliness.

These outstanding properties have been made possible by the new laser widefield
illumination with fully motorized elements for illumination in Total Internal
Reflexion (TIRF) and for the superresolution structured illumination (SR-SIM).
Electron multiplying CCDs guarantee highly sensitive detection. Intuitive operation
and comprehensive data analysis are implemented with the ZEN software.

The optimal combination for high-end research is offered by ELYRA in conjunction
with the Laser Scanning Microscope 780. However, PAL-M and SR-SIM can also be
combined with the LSM 710 laser scanning microscope.

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