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Human Adipokine Antibody Array

Introduction

R&D Systems Human Adipokine Array (Catalog # ARY024) is a rapid, sensitive, and economical tool used to simultaneously detect the relative levels of 58 different obesity-related molecules in a single sample. No specialized equipment is necessary

Simultaneously detect the levels of these obesity-related proteins in a single sample.

Adiponectin

FGF basic

M-CSF

Angiopoietin-1

FGF-19

MIF

Angiopoietin-2

Fibrinogen

Myeloperoxidase

Angiopoietin-like 2

Growth Hormone

Nidogen-1/Entactin

Angiopoietin-like 3

HGF

Oncostatin M

BAFF/BLyS

ICAM-I

Pappalysin-1/PAPP-A

BMP-4

IGFBP-2

PBEF/Visfatin

Cathepsin D

IGFBP-3

PCSK9

Cathepsin L

IGFBP-4

Pentraxin-3

Cathepsin S

IGFBP-6

Pref-1/DLK-1/FA1

CCL2/MCP-1

IGFBP-rp1/IGFBP-7

RAGE

CCL5/RANTES

IL-1 beta

Resistin

Chemerin

IL-6

Serpin A12

Complement Factor D

IL-10

Serpin A8/AGT

C-Reactive Protein

IL-11

Serpin E1/PAI-1

CXCL8/IL-8

LAP (TGF-ß1)

TIMP-1

DPPIV/CD26

Leptin

TIMP-3

Endocan

LIF

TNF-alpha

EN-RAGE

Lipocalin-2/NGAL

VEGF

Fetuin B

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, serum, plasma, saliva, urine, or human milk samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.

Kit Contents

4 Array Membranes

4-Well Multi-dish

Array Buffers

Wash Buffer

Detection Antibody Cocktail

Streptavidin-HRP

Chemiluminescent Detection Reagents

Transparency Overlay Template

Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the product datasheet.