Application Information: Adiponectin antibody [B479M]

Specifications: Adiponectin antibody [B479M]

Full Name

adiponectin, C1Q and collagen domain containing

Product Description

Mouse Monoclonal antibody [B479M] to Adiponectin

Background

This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Synonyms

ACDC, ACRP30, APM-1, APM1, GBP28, adiponectin

Specificity

Adiponectin

Host

Mouse

Clonality

Monoclonal

Clone Name

B479M

Target

Adiponectin

Isotype

IgG2a

Species Reactivity

Human

Storage Conditions: Adiponectin antibody [B479M]

Storage Buffer

PBS, pH 7.4;0.1% Sodium azide as preservative

Storage Instruction

Upon receipt, store at –20°C. Avoid multiple freeze/thaw cycles.

Notes

For in vitro research use only. Not intended for any diagnostic or therapeutic purpose. Not suitable for human or animal consumption.

Why is the observed Western Blot band size different from predicted size?

The predicted M.W. is based on protein sequence analysis; however, some factors might lead to an observed band size that is different from the predicted size. The reasons might include:
1.Post-translational modification (PTM):
a. Some post-translational modifications might lead to increased protein size, including
phosphorylation, acetylation, methylation, glycosylation, sumoylation, ubiquitination,
etc.
b. Some post-translational modifications might lead to decreased protein size including
phosphatidylethanolamine conjunction (e.g. LC3-II)
c. Some proteins may be cleaved to form an active or mature form; this process will
lead to a decreased protein size (e.g. Notch activation, Caspase activation, etc.)
d. Some websites provide useful PTM information
i.HPRD http://www.hprd.org/
ii.ProSite http://www.expasy.org/prosite/
iii.ELM http://elm.eu.org/
iv.CBS data sets http://www.cbs.dtu.dk/databases/
v.CBS prediction Servers http://www.cbs.dtu.dk/services/
2.mRNA splice variants (Isoforms):
Through alternative splicing, one gene can generate different proteins with different M.W. Regulation of alternative splicing depends upon cell type, conditions, etc.
3.Multimerization:
Some proteins could form dimers or multimers, increasing the M.W. This phenomenon usually can be found in reducing gel condition; however, strong interactions may still be seen with higher molecular weight proteins even in denaturing gel.
4.Protein charge:
The observed size could also potentially be influenced by the protein charge
5.Different species:
Different species likely have different protein sequence and PTM, which can lead to a different protein M.W.