JAPER

RPHPLC method for Simultaneous Estimation of Lansoprazole and aspirin in Bulk and Laboratory Mixture

Sadhana Rajput Suraj Fanse

Abstract

A simple, rapid, accurate and precise isocratic RP-HPLC method was developed for
simultaneous estimation of Aspirin and Lansoprazole in bulk and prepared laboratory
sample. Chromatographic separation was performed on Kromasil C-18 column (Column
dimensions: 250 mm x 4.6 mm, 5 μm) using 10mM Phosphate buffer pH 3: Acetonitrile=
55:45 as mobile phase at a flow rate of 1 mL/min. Retention time for Aspirin and
Lansoprazole was 4.3min and 6.1 min respectively. The reliability and analytical
performance of the proposed RP-HPLC method was statistically validated with respect to
linearity, precision, accuracy, robustness, specificity, limit of detection and quantification as
per ICH Q2 (R1) guidelines. The mean percentage recovery for Aspirin and Lansoprazole
was found to be 100.49 % and 100.5 % respectively. The proposed RP-HPLC method was
successfully applied for analysis of laboratory mixture. The mean percentage assay values
for Aspirin and Lansoprazole was reported to be 100.73 % and 99.5 % respectively. The
developed and validated RP-HPLC method was used for estimation of Aspirin and
Lansoprazole in a run time less than 10 minutes.