Hi to all in ether-land,
I have a couple of DNA samples that I have extracted from either EDTA-
or heparinized blood samples and unsuprisingly they are just about
impossible to amplify using PCR. I have tried using microcons and
ethanol precipitating the DNA to remove the heparin and EDTA, but to no
avail (I can amplify it just, but not always). I have heard that you
can add heparinase to a DNA solution to remove heparin, and you can heat
treat a DNA solution to remove EDTA. Has anybody tried these tricks?
Do they really work?
Thanks, Dan
Dan Andrews
John Curtin School of Medical Research
Canberra, AUSTRALIA