Shown are the results of the third-pass (n = 6) inductively coupled plasma-atomic emission spectroscopy (ICP-AES) analysis. Sheet
1: Normalized concentrations in parts per million (ppm) for each of the 13 elements
assayed. Sheet 2: z scores are reported for each mutant and each element. These values are the number
of standard deviations that the mutant value differed from the mean of the wild-type
samples included in that experiment. Sheet 3: The z score data from Sheet 2 were converted into trinary data. A value of 1 was assigned
if the z score was ≥2.5 and -1 if the z score was ≤-2.5. Values of 0 were assigned for values that fell between those boundaries.

Shown are the results of an inductively coupled plasma-atomic emission spectroscopy
(ICP-AES) analysis (n = 6) of V-ATPase subunit mutants grown in yeast extract-peptone-dextrose (YPD) medium
with metal supplements. Normalized accumulation is reported in parts per million (ppm)
and z scores are reported for each element. Calcium values from this experiment are not
reported due to a high background of this element in these particular samples.

Shown are the results of an inductively coupled plasma-atomic emission spectroscopy
(ICP-AES) analysis (n = 6) of wild-type cells grown in yeast extract-peptone-dextrose (YPD) medium with
or without the metal supplements. Normalized accumulation is reported in parts per
million (ppm) and z scores are reported for each element.