AnkyrinG-dependent nodal organization, maturation, and long-term stability. A, Perinatal ablation of AnkG results in nodes that lack AnkG but assemble NF186, NaV channels, and βIV Spec. Without AnkG, nodes fail to mature and lose NaV channels and βIV Spec with only minor effects of NF186. Mice die at approximately P40. B, Juvenile ablation of AnkG results in AnkG loss from the majority of nodes within 1 mpi. In the absence of AnkG, βIV Spec stability is primarily affected, followed by NaV channels, with only minor effects on NF186. Mice die at ∼10 mpi.