Venous access devices are used to manage patients undergoing long term treatment; especially chemotherapy.Though it has many advantages; they are not free from complications. Sepsis; thrombotic complications; local tissue damages are more common complications. AIM-the main of study was to assess the impact of structure education on knowledge and practice regarding venous access device among nurses.Methodology- The quasi-experimental study with pre-test-post-test design was used.The sample size for the study was 60. In this; 30 were in experimental group and 30 were in control group.The samples were selected from the cancer units. FINDINGS-The maximum of 43.33 % of samples scored between 0-13 (Poor) in the knowledge pre-test before structured education to the experimental group and 65 % samples scored between 18-25 ( Good) in the post-test after structured to experimental group. In control group; the mean score of knowledge score was increased from 15.3 to 16.4 in the post test. In Experimental group of the study; the mean score of knowledge regarding venous access device care was increased from 14.6 to 21.3 in the post-test after structured education. In experimental group; the maximum of 75 % of samples scored between 14-17 (Average) in the practice pre-test before structured education and 48.33 % samples scored between 18-25 (Good) score in the practice post-test score after structured education. In control group; the mean score of practice score was increased from 15.4 to 15.5 in the post test. CONCLUSION: From all the above findings it can be concluded that the structured education was effective on knowledge and practice of staff nurses regarding venous access device care.