Growth factors in small oocyte development: Proteomics and genomic approaches

Salient Achievements

In addition to the exchange visits from both the countries, research was done on the role of amphiregulin and neuregulin on oocyte cumulus expansion and biochemical profiling of different size categories of ovarian follicles

Transcript profiling of GDF-9, FGF-2, FGF-10, FGF-8 and GAPDH in immature goat oocytes was done. Targeted fragments of these transcripts were PCR amplified, cloned and sequenced.

Indo - Australian Project (ICAR-ACIAR)

Increasing the productivity of cattle in India and Australia through rumen fungal treatments

Salient Achievements

In addition to the training received by Indian scientists and exchange of visits from both the countries, anaerobic fungi were isolated from a large number of small and large ruminants, zoo ruminants and large herbivores. The rumen liquor samples were restricted to animals of the Institute while the dung samples were collected from various districts of Karnataka as well as other states. All the five recognized genera of gut fungi were successfully isolated. Existence of Cyllamyces,a sixth genera was reported in Buffaloes for the first time in the Indian sub-continent. Four of the superior fiber degrading cultures selected based on results of in vitro gas production were tested for their relative abilities to degrade plant fiber in cattle feeding trials. A feeding trial was carried out in Holstein heifers using three groups of six animals as follows: (1) basal diet (control); (2) basal diet plus 6.6 g/day anhydrous sodium sulphate (sulphate; 1.45 g S/day) and (3) basal diet plus 8.0 g/day anhydrous sodium MPS (Na-MPS; 2.88 g S/day).Supplementation of a S-deficient low quality roughage diet, with either MPS or sodium sulphate in conjunction with compensatory N, improved rumen fermentation through an apparent increase in microbial protein synthesis.

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