DescriptionMuscle wasting disorders like atrophy and sarcopenia are characterized by compromised skeletal muscle mass and function. One of the many causes of muscle wasting reported is the failure of satellite cells to fuse and form myofibres. Satellite cells are an integral part of skeletal muscle regeneration because of their self-renewal and differentiation abilities. Muscle regeneration research with respect to satellite cells has stimulated interest in discovering factors that enhance proliferation and differentiation as a first step in the regenerative process. The importance of nutrition in regulating muscle mass and muscle protein synthesis is well established. However, limited literature is available regarding the role of nutrition in muscle regeneration; specifically with respect to satellite cells. As for nutrition, chicken egg yolk appears to be a promising candidate. Egg yolks are rich in nutrients such as vitamins, minerals, amino acids and proteins which can provide sustained supply of nutrition to the cells in-vitro. In the present study, we tested the effect of different fractions and concentrations of egg yolk extract on C2C12 myoblasts. For this purpose, egg yolk extract was supplemented in growth media and cells were grown in this nutrient rich media for one week. Cell viability, fluorescence imaging and mRNA expression of muscle specific genes were analyzed at specific time points of proliferation and differentiation stages of myoblast. The study results showed that supplementation of the egg yolk extract in media enhanced proliferation and differentiation of myoblasts in a dose dependent manner which was analyzed by increase in cell viability, number of nuclei, number of myofibres and mRNA expression of muscle specific genes such as MyoD and myogenin.