POSTER 176 -
A COMPLETED MOUSE GENETIC MAP: THE COMPREHENSIVE T31 RADIATION
HYBRID MAP CROSS REFERENCED AT LOD > 6 TO THE RECOMBINATION
MAP FORMS A BASIS FOR SUPPORT OF THE SEQUENCE ASSEMBLIES

LB Rowe
The Jackson Laboratory

Barter ME,
Kelmenson JA, Eppig JT
The Jackson Laboratory

We have collated the mouse T31 Radiation Hybrid (RH)
data from all public sources into a single comprehensive map. As
of Fall 2002 The Jackson Laboratory T31 mouse RH database (http://www.jax.org/resources/documents/cmdata/rhmap/RHIntro.html)
contained about 4,000 distinct microsatellite anchor markers typed
by several independent laboratories, over 17,000 unique expressed
sequence tag (EST) loci mapped by three international genome centers,
and hundreds of other loci. To date approximately one third of the
mapped ESTs have identified gene functions. About 400 different laboratories
worldwide have contributed RH data to this effort.To maximize the
level of confidence for the mouse map and its usefulness in supporting
the whole genome sequence assemblies, we have built a complete set
of chromosome framework maps of known-order anchor markers between
the RH and The Jackson Laboratory backcross panels. This recombination-based
map contains about 6,000 loci mapped in a 188 animal interspecific
backcross that has been in use by the community for the past nine
years. The complete raw data and maps from this cross are available
in a public database from http://www.jax.org/resources/documents/cmdata.
The frameworking project has resulted in the correction of some genomic
regions that were intially misordered in the RH data, the identification
of mismapping D_Mit_ anchor loci, the detection of some data errors
and omissions in the recombination map, and provides a high density
of cross-links between the recombination and radiation hybrid maps
that facilitate accurate genome-wide map construction. Future work
will include direct order and distance correlation between this map
and the mouse genome sequence.