JacobGonzález-Solís

Associate Professor, doctor of biological sience

I am a researcher in the fields of evolutionary, population and environmental biology. My research aims to understand the key processes driving the spatial and temporal dynamics of animal populations. I mainly focus on movement ecology, symbiotic interactions and marine environmental chemistry, using seabirds as the main study model. I am concerned with the loss of biodiversity and therefore all my research intends to contribute to the field of conservation biology. My research includes observational and experimental field studies integrating multidisciplinary approaches and tools, such as molecular, stable isotope, physiological, microbiological and contamination analyses. I am leading a team of two posdocts, 9 PhD students and a number of MSc and undergrad students. We instrument animals with different sort of devices, such as GPSs, GLSs, wet-dry sensors and accelerometers, to track their foraging and migratory movements and behaviour. Our tracking data are contributing to several processes of identification and assessment of important bird areas at sea (mIBAs), marine protected areas and the study of seabird-fishery interactions in the Atlantic and Mediterranean regions. Our activities also include building capacity in developing countries by training students, technicians and field assistants as well as increasing public awareness on anthropogenic threats to seabirds and to the marine environment. Our main research sites are in the Macaronesic archipelagos, particularly Cape Verde and Canary Islands, as well as in some Antarctic and subantarctic localities. Long-term studies in ecological research are essential to document key changes in the environment and therefore we monitored some of these study sites for >10 years now. We hold a large network of international research collaborations in France, UK, Germany, Portugal, Iceland, Cape Verde, Senegal, South Africa and Chile. We also collaborate with a number of NGOs in Spain and Cape Verde.

Mecanismos Evolutivos del Comportamiento y Montaje de Comunidades

Biodiversidad de Aves Marinas

Topics.

Movement Ecology

Movement is a fundamental trait in animal life shaped by an infinity of ecological and evolutionary processes.

Movement is a fundamental trait in animal life shaped by an infinity of ecological and evolutionary processes. It is a key trait for basic activities such as foraging for food, escaping predators, finding a partner or migrating to better places. Animal movement has strong consequences on all levels of organization, from individual fitness to population dynamics and community and ecosystem structure as well as on conservation and human health. It is therefore essential to improve our knowledge on the movement of animals and the factors that influence them. Movement is linked to habitat features both, physical and biological, and therefore the study of the interactions between these traits and animal movement will help us understanding the main factors driving it. In this context, we focus on (1) improving segmentation methods to understand behavioural modes based on the combination of information obtained from different tracking and sensing devices; (2) understanding the degree of individual specialization in several seabird species and what are the main factors driving it as well as understanding the ecological and conservation consequences; (3) understanding migratory connectivity and the importance of seasonal interactions and carry-over effects on different fitness components and ultimately population dynamics; (4) understanding the role of plasticity and microevolution on the adaptive potential of the migratory behaviour and the genetic/learning basis of animal movement.

Conservation Biology

A major challenge in our world is the loss of biodiversity, and marine ecosystems and seabirds do not escape this tendency.

A major challenge in our world is the loss of biodiversity, and marine ecosystems and seabirds do not escape this tendency. In fact, over the last decades, seabird conservation status has worsened more than any other group of birds. The huge mobility of seabirds makes them particularly vulnerable, since they can travel for even thousands of kilometres and be exposed to threats in remote areas we even do not know. At sea, the most important threat are different type of fisheries, in particular seabird bycatch in longlining. Therefore there is an urgent need to understand seabird movements and the threats they are suffering. In this context, we focus on (1) understanding the influence of fisheries on seabirds movements and main factors driving their interactions; (2) finding method to mitigate seabird by-catch in longliners that make this fishery sustainable and environmentally friendly; (3) contributing to the identification and definition of marine protected areas in the Atlantic and the Mediterranean, by providing new tracking data on several seabird species; (4) evaluating the spatial and temporal consistency in the foraging and wintering areas at individual and population levels.

Symbiotic Interactions

The close and persistent association between two or more phylogenetically distant organisms is extraordinarily interesting from an evolutionary perspective.

(149) Do humans spread zoonotic enteric bacteria in Antarctica? Science of the Total Environment 654:190-196.

Reports of enteric bacteria in Antarctic wildlife have suggested its spread from people to seabirds and seals, but evidence is scarce and fragmentary. We investigated the occurrence of zoonotic enteric bacteria in seabirds across the Antarctic and subantarctic region; for comparison purposes, in addition to seabirds, poultry in a subantarctic island was also sampled. Three findings suggest reverse zoonosis from humans to seabirds: the detection of a zoonotic Salmonella serovar (ser. Enteritidis) and Campylobacter species (e.g. C. jejuni), typical of human infections; the resistance of C. lari isolates to ciprofloxacin and enrofloxacin, antibiotics commonly used in human and veterinary medicine; and most importantly, the presence of C. jejuni genotypes mostly found in humans and domestic animals but rarely or never found in wild birds so far. We also show further spread of zoonotic agents among Antarctic wildlife is facilitated by substantial connectivity among populations of opportunistic seabirds, notably skuas (Stercorarius). Our results highlight the need for even stricter biosecurity measures to limit human impacts in Antarctica.

(148) Foraging ecology of tropicbirds breeding in two contrasting marine environaments in the tropical Atlantic. Marine Ecology Progress Series 607:221-236.

Studying the feeding ecology of seabirds is important not only to understand basic aspects of their ecology and threats but also for the conservation of marine ecosystems. In this regard, tropical seabirds have been relatively neglected, and in particular the trophic ecology of tropicbirds is scarcely known. We combined GPS tracking, environmental variables and sampling of regurgitates during incubation and brooding to understand the feeding ecology of red-billed tropicbirds Phaethon aethereus as well as how foraging strategies may change between 2 contrasting marine environments: a coastal island in the Canary Current upwelling (Iles de la Madeleine) and an oceanic island in the middle of the south Atlantic (St Helena). Tropicbirds breeding on the Iles de la Madeleine headed west, foraging on and beyond the shelf slope, probably to associate with subsurface predators which bring pelagic fish close to the surface. Birds from St Helena showed a greater foraging effort and a strong attraction to areas with the greatest species richness of Scombridae, possibly due to a greater difficulty in finding prey in the oligotrophic oceanic waters. Tropicbirds ranged much beyond the extension of the protected areas around their colonies, indicating that current protected areas are insufficient for these populations. We found no evidence to suspect direct mortality of tropicbirds in regional fisheries, but overexploitation of small epipelagic fish and tuna may decrease feeding opportunities and lead to competition with fisheries. The substantial differences in foraging behaviour demonstrated by individuals from both colonies indicates that caution should be taken when extrapolating foraging patterns of tropical seabirds breeding in contrasting oceanographic environments.

(147) Multi-isotopic assessments of spatio-temporal variability of diet: the case of two sympatric gulls in the Western Mediterranean. Marine Ecology Progress Series 606:201-214.

In predator populations, changes in foraging behaviour in response to spatio-temporal variability of prey are expected. Prey depletion might cause trophic niche widening in generalist species, but not in specialists, which are expected to increase their foraging effort without diet shifts. In sympatric species feeding on similar resources, reduced food availability can increase interspecific competition and cause trophic niche segregation. To understand these processes, we studied the spatio-temporal variability in diet and niche width in 2 sympatric gull species, the yellow-legged gull Larus michahellis and Audouin’s gull Ichthyaetus audouinii, which have experienced exponential growth in recent decades due to an increase in anthropogenic food subsidies. We sampled feathers from chicks of both species in several colonies along the western Mediterranean from 2009 to 2011 and performed stable isotope analysis of carbon, nitrogen and sulphur on these feathers. Bayesian modelling shows that both species displayed opportunistic behaviour if different types of resources were available, but could also narrow their trophic niche if 1 resource was abundant. We also provide evidence of trophic segregation between the 2 gull species, suggesting the occurrence of interspecific competition for food. Our meta-population approach provides a comprehensive view of the trophic ecology and the competitive interactions of these gull species. We emphasize the usefulness of 3-dimensional isotope analyses to correctly assess spatio-temporal variability in trophic behaviour of predator species, revealing differences that would remain hidden in single population studies or when using only the isotopic ratios of 2 elements.

Knowing the spatial scales at which effective species management can be implemented is fundamental for conservation. This is especially important for mobile species, which can be exposed to multiple threats across large geographic areas, but the space use requirements of different species can vary to an extent that might render some management approaches inefficient. We examined the space use patterns of seabirds and provide guidance on whether conservation management approaches should be tailored for taxonomic groups with different movement characteristics. We synthesised tracking data from 5419 adult breeding individuals of 52 different species in ten
families that were collected in the Atlantic between 1998 and 2017. Two key aspects of spatial distribution were quantified, namely how far seabirds ranged from their colony, and to what extent individuals from the same colony used the same areas at sea. There was evidence for substantial differences in patterns of space use among the ten studied seabird families, indicating that several alternative conservation management approaches are needed. Several species exhibited large foraging ranges and little aggregation at sea, indicating that area-based conservation solutions would have to be extremely large to adequately protect such species. The results highlight that short-ranging and aggregating species such as cormorants, auks, some penguins, and gulls would benefit from conservation approaches at relatively small spatial scales during their breeding season. However, improved regulation of fisheries, bycatch, pollution and other threats over large spatial scales will be needed for wide-ranging and dispersed species such as albatrosses, petrels, storm petrels and frigatebirds.

(145) Costs of breeding are rapidly buffered and do not affect migratory behaviour in a long-lived bird species. Ecology. In press.

Life history theory states that individual fitness in one stage of life is conditioned by what occurred in previous stages. In migratory species, reproductive effort during breeding has often been found to influence body condition, molt schedule, self-provisioning and migration of individuals in subsequent seasons (i.e., carryover effects of breeding). However, there is a current uncertainty in understanding how long-distance migrants trade off among such energy-demanding activities (i.e., breeding, molting and migrating). To provide evidence to the field, we experimentally reduced the parental effort of a long-lived Procellariform, the Cory's shearwater (Calonectris borealis), by inducing failure at the incubation stage. Treatment and control birds were tracked during their subsequent migration by means of light-level and immersion loggers and sampled for six specific feathers (molted at different periods along the migratory cycle) upon the recovery of the loggers 1 yr later. Feathers were used to perform stable isotope analysis (SIA) and determine corticosterone levels (CORT). By these means, we evaluated the effect of breeding effort on migratory strategy, at-sea activity patterns, molt patterns, and levels of stress experienced by birds along the non-breeding period. We did not detect any difference between birds in the induced failure group and successful breeders in terms of spatio-temporal distribution: all birds shared common foraging areas throughout the study period and the timing of major phenological events did not differ. Failed birds significantly advanced their molt, as revealed by SIA and flying activity patterns. The stress levels of failed birds, inferred through CORT concentrations in feathers, were found to be consistently lower than in successful breeders, through the end of the breeding to the non-breeding period. Thus, we provide robust evidence that the costs of reproduction can be physiologically mediated from the breeding to the non-breeding period through molting schedules and CORT levels. However, we failed to detect clear effects on migratory behavior or subsequent breeding success, suggesting that costs of breeding in long-lived species may be rapidly buffered during the post-breeding period, as would be expected from life history theory.

High numbers of seabirds are killed annually worldwide in longline fisheries. In the Mediterranean, this mortality is seriously affecting the viability of seabird populations, in particular of the three endemic shearwaters. Even so, there is currently no specific seabird mitigation requirements for the longline fleet operating in this area. From 2013 to 2014, we assessed the efficiency and practical applicability of four mitigation measures on artisanal demersal longliners targeting European hake (Merluccius merluccius) in the western Mediterranean: night setting, tori line, weighted lines and artificial baits. We performed fifty-two pairs of experimental (with the tested mitigation measure) and control settings (without any measure), and compared their effects on seabird interactions and fish catches. In addition, we estimated the longline sink rates and the seabird access area to baited hooks in different longline configurations. Night setting reduced bycatch risk without affecting target and non-commercial fish catches. The tori line may have reduced the bycatch risk by displacing bait attacks beyond the end of the line, but at this distance shearwaters could still access to the baits and the streamers did not deter birds under calm wind conditions. Weighted lines increased sink rate, but it resulted in only a minor reduction of the seabird access window to baited hooks and led to some operational problems during the setting. Artificial baits substantially reduced commercial catches. Moreover, the seabird access to the baited hooks was influenced by the longline configuration, the setting speed and the relative position to the floats and weights. So far, night setting stands out as the best mitigation measure for reducing bycatch levels without compromising target catches in demersal longliners. Ideally, these results should be confirmed in longliners targeting species other than European hake.

Reproductive timing in many taxa plays a key role in determining breeding productivity1, and is often sensitive to climatic conditions2. Current climate change may alter the timing of breeding at different rates across trophic levels, potentially resulting in temporal mismatch between the resource requirements of predators and their prey3. This is of particular concern for higher-trophic-level organisms, whose longer generation times confer a lower rate of evolutionary rescue than primary producers or consumers4. However, the disconnection between studies of ecological change in marine systems makes it difficult to detect general changes in the timing of reproduction5. Here, we use a comprehensive meta-analysis of 209 phenological time series from 145 breeding populations to show that, on average, seabird populations worldwide have not adjusted their breeding seasons over time (-0.020?days?yr-1) or in response to sea surface temperature (SST) (-0.272?days?°C-1) between 1952 and 2015. However, marked between-year variation in timing observed in resident species and some Pelecaniformes and Suliformes (cormorants, gannets and boobies) may imply that timing, in some cases, is affected by unmeasured environmental conditions. This limited temperature-mediated plasticity of reproductive timing in seabirds potentially makes these top predators highly vulnerable to future mismatch with lower-trophic-level resources.

(152) Population size and breeding phenology of red-billed tropicbirds (Phaethon aethereus) on Iles de la Madeleine, Senegal. Waterbirds. In press.

Diop, N., C.T.Ba, P.I.Ndiaye, T.Militão, J.González-Solís (2019)

The breeding phenology and nest site distribution of Red-billed Tropicbirds (Phaethon aeth-
ereus) was monitored from 6 June 2014 to 18 May 2016 on Parc National des Iles de la Madeleine, Senegal.
During this period, this study site was visited every 15 days to record active nests and their contents (eggs or
chicks). We found up to 76 nest sites but only 49 were active in 2014-2015 and 45 in 2015-2016. Red-billed
Tropicbirds bred throughout the year but numbers of active nests peaked from October to January, which may
be related to the seasonality of ocean upwelling. Nests were aggregated in four areas and their distribution and
occupancy may be related to the direction of the wind during the seasonal peak of reproduction from October
to May. The breeding success was generally high (62.9% in 2014-2015 and 47.3% in 2015-2016) compared
to other colonies breeding in less productive waters. Given the singularity and small size of this population,
monitoring, management and strict protection is clearly needed to guarantee its viability.

(151) Exposure of yellow-legged gulls to Toxoplasma gondii along the Western Mediterranean coasts: tales from a sentinel. International Journal of Parasitology. In press.

Efficiently tracking and anticipating the dynamics of infectious agents in wild populations requires the gathering of large numbers of samples, if possible at several locations and points in time, which can be a challenge for some species. Testing for the presence of specific maternal antibodies in egg yolks sampled on the colonies could represent an efficient way to quantify the exposure of breeding females to infectious agents, particularly when using an abundant and widespread species, such as the yellow-legged gull (Larus michahellis). We used such an approach to explore spatio-temporal patterns of exposure to Toxoplasma gondii, a pathogenic protozoan responsible of toxoplasmosis in humans and other warm blooded vertebrates. First, we tested the validity of this approach by exploring the repeatability of the detection of specific antibodies at the egg level using two different immunoassays and at the clutch level using an occupancy model. Then, samples gathered in 15 colonies from France, Spain and Tunisia were analysed using an immunoassay detecting antibodies specifically directed against T. gondii. Prevalence of specific antibodies in eggs was overall high while varying significantly among colonies. These results revealed that T. gondii circulated at a large spatial scale in the western Mediterranean yellow-legged gull population, highlighting its potential role in the maintenance community of this parasite. Additionally, this study illustrates how species commensal to human populations like large gulls can be used as wildlife sentinels for the tracking of infectious agents at the human-wildlife interface, notably by sampling eggs.

Bird migration studies have been increasing recently thanks to the miniaturization of tracking devices. However, for the smallest pelagic species, tracking methodologies have remained impractical and thus, important gaps in knowledge still exist. In the case of the European Storm Petrel (Hydrobates pelagicus), while Atlantic populations are thought to overwinter along the south-western African coast, the wintering grounds of Mediterranean birds remain more enigmatic. We performed stable isotope analysis (SIA) on P1, S8 and P10 feathers from 33 adult birds captured in three Atlantic colonies and 156 adult birds in seven western Mediterranean colonies to infer their wintering areas. In addition, we collated all observational field data, both from peer-reviewed publications and the grey literature, to complement our inferences from SIA. Isotopic profiles of feathers moulted at the breeding grounds (P1) differed between birds captured at northern Atlantic and Canary Islands colonies, but were similar for feathers moulted at wintering grounds (S8 and P10), indicating a low migratory connectivity. Isotopic values of feathers from western Mediterranean birds differed from those of Atlantic birds and showed Mediterranean values for all feathers, indicating that birds remain in Mediterranean waters during the winter. Variance in the isotopic values was greater in wintering than in breeding feathers, suggesting that birds disperse over larger areas. Isotopic values of feathers moulted during the non-breeding period could match a post-breeding movement towards the southern and eastern Mediterranean. This inference matches the distribution of the few winter reports, which are mainly concentrated in south-central Mediterranean, mostly in the Tunisian Platform. Our results suggest this region as the main wintering grounds for Mediterranean Storm Petrel.

(149) Do humans spread zoonotic enteric bacteria in Antarctica? Science of the Total Environment 654:190-196.

Reports of enteric bacteria in Antarctic wildlife have suggested its spread from people to seabirds and seals, but evidence is scarce and fragmentary. We investigated the occurrence of zoonotic enteric bacteria in seabirds across the Antarctic and subantarctic region; for comparison purposes, in addition to seabirds, poultry in a subantarctic island was also sampled. Three findings suggest reverse zoonosis from humans to seabirds: the detection of a zoonotic Salmonella serovar (ser. Enteritidis) and Campylobacter species (e.g. C. jejuni), typical of human infections; the resistance of C. lari isolates to ciprofloxacin and enrofloxacin, antibiotics commonly used in human and veterinary medicine; and most importantly, the presence of C. jejuni genotypes mostly found in humans and domestic animals but rarely or never found in wild birds so far. We also show further spread of zoonotic agents among Antarctic wildlife is facilitated by substantial connectivity among populations of opportunistic seabirds, notably skuas (Stercorarius). Our results highlight the need for even stricter biosecurity measures to limit human impacts in Antarctica.

(148) Foraging ecology of tropicbirds breeding in two contrasting marine environaments in the tropical Atlantic. Marine Ecology Progress Series 607:221-236.

Studying the feeding ecology of seabirds is important not only to understand basic aspects of their ecology and threats but also for the conservation of marine ecosystems. In this regard, tropical seabirds have been relatively neglected, and in particular the trophic ecology of tropicbirds is scarcely known. We combined GPS tracking, environmental variables and sampling of regurgitates during incubation and brooding to understand the feeding ecology of red-billed tropicbirds Phaethon aethereus as well as how foraging strategies may change between 2 contrasting marine environments: a coastal island in the Canary Current upwelling (Iles de la Madeleine) and an oceanic island in the middle of the south Atlantic (St Helena). Tropicbirds breeding on the Iles de la Madeleine headed west, foraging on and beyond the shelf slope, probably to associate with subsurface predators which bring pelagic fish close to the surface. Birds from St Helena showed a greater foraging effort and a strong attraction to areas with the greatest species richness of Scombridae, possibly due to a greater difficulty in finding prey in the oligotrophic oceanic waters. Tropicbirds ranged much beyond the extension of the protected areas around their colonies, indicating that current protected areas are insufficient for these populations. We found no evidence to suspect direct mortality of tropicbirds in regional fisheries, but overexploitation of small epipelagic fish and tuna may decrease feeding opportunities and lead to competition with fisheries. The substantial differences in foraging behaviour demonstrated by individuals from both colonies indicates that caution should be taken when extrapolating foraging patterns of tropical seabirds breeding in contrasting oceanographic environments.

(147) Multi-isotopic assessments of spatio-temporal variability of diet: the case of two sympatric gulls in the Western Mediterranean. Marine Ecology Progress Series 606:201-214.

In predator populations, changes in foraging behaviour in response to spatio-temporal variability of prey are expected. Prey depletion might cause trophic niche widening in generalist species, but not in specialists, which are expected to increase their foraging effort without diet shifts. In sympatric species feeding on similar resources, reduced food availability can increase interspecific competition and cause trophic niche segregation. To understand these processes, we studied the spatio-temporal variability in diet and niche width in 2 sympatric gull species, the yellow-legged gull Larus michahellis and Audouin’s gull Ichthyaetus audouinii, which have experienced exponential growth in recent decades due to an increase in anthropogenic food subsidies. We sampled feathers from chicks of both species in several colonies along the western Mediterranean from 2009 to 2011 and performed stable isotope analysis of carbon, nitrogen and sulphur on these feathers. Bayesian modelling shows that both species displayed opportunistic behaviour if different types of resources were available, but could also narrow their trophic niche if 1 resource was abundant. We also provide evidence of trophic segregation between the 2 gull species, suggesting the occurrence of interspecific competition for food. Our meta-population approach provides a comprehensive view of the trophic ecology and the competitive interactions of these gull species. We emphasize the usefulness of 3-dimensional isotope analyses to correctly assess spatio-temporal variability in trophic behaviour of predator species, revealing differences that would remain hidden in single population studies or when using only the isotopic ratios of 2 elements.

Knowing the spatial scales at which effective species management can be implemented is fundamental for conservation. This is especially important for mobile species, which can be exposed to multiple threats across large geographic areas, but the space use requirements of different species can vary to an extent that might render some management approaches inefficient. We examined the space use patterns of seabirds and provide guidance on whether conservation management approaches should be tailored for taxonomic groups with different movement characteristics. We synthesised tracking data from 5419 adult breeding individuals of 52 different species in ten
families that were collected in the Atlantic between 1998 and 2017. Two key aspects of spatial distribution were quantified, namely how far seabirds ranged from their colony, and to what extent individuals from the same colony used the same areas at sea. There was evidence for substantial differences in patterns of space use among the ten studied seabird families, indicating that several alternative conservation management approaches are needed. Several species exhibited large foraging ranges and little aggregation at sea, indicating that area-based conservation solutions would have to be extremely large to adequately protect such species. The results highlight that short-ranging and aggregating species such as cormorants, auks, some penguins, and gulls would benefit from conservation approaches at relatively small spatial scales during their breeding season. However, improved regulation of fisheries, bycatch, pollution and other threats over large spatial scales will be needed for wide-ranging and dispersed species such as albatrosses, petrels, storm petrels and frigatebirds.

(145) Costs of breeding are rapidly buffered and do not affect migratory behaviour in a long-lived bird species. Ecology. In press.

Life history theory states that individual fitness in one stage of life is conditioned by what occurred in previous stages. In migratory species, reproductive effort during breeding has often been found to influence body condition, molt schedule, self-provisioning and migration of individuals in subsequent seasons (i.e., carryover effects of breeding). However, there is a current uncertainty in understanding how long-distance migrants trade off among such energy-demanding activities (i.e., breeding, molting and migrating). To provide evidence to the field, we experimentally reduced the parental effort of a long-lived Procellariform, the Cory's shearwater (Calonectris borealis), by inducing failure at the incubation stage. Treatment and control birds were tracked during their subsequent migration by means of light-level and immersion loggers and sampled for six specific feathers (molted at different periods along the migratory cycle) upon the recovery of the loggers 1 yr later. Feathers were used to perform stable isotope analysis (SIA) and determine corticosterone levels (CORT). By these means, we evaluated the effect of breeding effort on migratory strategy, at-sea activity patterns, molt patterns, and levels of stress experienced by birds along the non-breeding period. We did not detect any difference between birds in the induced failure group and successful breeders in terms of spatio-temporal distribution: all birds shared common foraging areas throughout the study period and the timing of major phenological events did not differ. Failed birds significantly advanced their molt, as revealed by SIA and flying activity patterns. The stress levels of failed birds, inferred through CORT concentrations in feathers, were found to be consistently lower than in successful breeders, through the end of the breeding to the non-breeding period. Thus, we provide robust evidence that the costs of reproduction can be physiologically mediated from the breeding to the non-breeding period through molting schedules and CORT levels. However, we failed to detect clear effects on migratory behavior or subsequent breeding success, suggesting that costs of breeding in long-lived species may be rapidly buffered during the post-breeding period, as would be expected from life history theory.

Feather mites are useful models for studying speciation due to their high diversity and strong degree of host specialization. However, studies to date have focused on the evolution of higher-level mite taxa while much hidden diversity likely occurs at the level of host genera and species. In this study, we examined the diversity and evolution of feather mites infesting six sympatric seabird species from six genera, breeding in the Cape Verde archipelago. We report 32 feather mite morphospecies categorized into 10 genera and three families, of which nine correspond to new, undescribed species. Molecular data corroborated morphological species descriptions, except for two morphologically-cryptic, but genetically distinct mite lineages related to Zachvatkinia oceanodromae and Laminalloptes simplex. Using these communities, we then applied a co-structure approach to test the contribution of ectosymbiont and host factors in driving feather mite evolution. Most seabird species hosted specific and unique feather mite species, even under sympatric conditions, and in general, feather mite species exhibited strong host-driven genetic structure. However, patterns of genetic differentiation were variable. That is, some mite species are more generalist than others and mite lineages/haplotypes can be shared by related seabird species. Interestingly, host-specific mites (e.g., Zachvatkinia spp.) tend to display much higher intra-specific diversity compared to more generalist mites (e.g., Microspalax and Plicatalloptes spp.). We discuss ectosymbiont and host life-history traits that might generate these patterns, such as host dispersal and breeding behavior and/or mite spatial and trophic specialization. Our findings highlight both the vast and largely unrecognized diversity of avian feather mites on seabirds, and the intrinsic complexity of the ecological processes underlying the evolution of these ectosymbionts.

The objective of the present study was to assess the presence of 17 per- and polyfluoroalkyl substances (PFAS) in blood of two closely related top predators, the Scopoli's (Calonectris diomedea) and Cory's (C. borealis) shearwaters that breed allopatrically in Mediterranean and Atlantic basins. Among PFAS, perfluorooctanesulfononic acid (PFOS) and long chain perfluorinated carboxylic acids (PFCA) were detected in all samples, highlighting somehow the ubiquity of such pollutants. Scopoli's shearwaters from the Mediterranean showed significant higher levels in all PFAS when compared with those levels determined in the colony of Cory's shearwater from the Atlantic Ocean. Moreover, significant differences also arose within the Mediterranean colonies, with the colony of the Central Mediterranean (Zembra Island in Tunisia) having the lowest levels of all PFAS. Thus, our study suggests a marked geographical pattern regarding the presence of such contaminants, and emphasizes the necessity to study PFAS accumulation in birds inhabiting temperate regions.

(142) Using marine isoscapes to infer movements of oceanic migrants: the case of Bulwer’s petrel (Bulweria bulwerii) in the Atlantic Ocean. PLoS ONE 13: e0198667.

Cruz-Flores, M., T.Militão, R.Ramos, J.González-Solís (2018)

Studying the movements of oceanic migrants has been elusive until the advent of several tracking devices, such as the light-level geolocators. Stable isotope analysis (SIA) offers a complementary approach to infer areas used year-round, but its suitability in oceanic environments remains almost unexplored. To evaluate SIA as a tool for inferring movements of oceanic migrants, we sampled an oceanic seabird, the Bulwer’s petrel, Bulweria bulwerii, in four breeding colonies spread along its Atlantic distribution. We first studied the species moulting pattern from 29 corpses collected in the colonies. Secondly, based on this moult knowledge, we selected three feathers from tracked birds to infer their breeding and non-breeding grounds using SIA: the 1st primary (P1), the 8th secondary (S8) and the 6th rectrix (R6) feathers. Birds migrated to two main non-breeding areas, the Central or the South Atlantic Ocean. P1 showed similar isotopic values among petrels from different breeding colonies, suggesting this feather is replaced early in the non-breeding period in a common area used by most birds, the Central Atlantic. S8 and R6 feathers correctly assigned 92% and 81%, respectively, of the birds to their non-breeding areas, suggesting they were replaced late in season, when birds were settled in their main non-breeding grounds. Our results showed that the isotopic baseline levels of the Central and South Atlantic are propagated through the food web until reaching top predators, suggesting these ratios can be used to infer the movement of long-distance migrants among oceanic water masses.

High numbers of seabirds are killed annually worldwide in longline fisheries. In the Mediterranean, this mortality is seriously affecting the viability of seabird populations, in particular of the three endemic shearwaters. Even so, there is currently no specific seabird mitigation requirements for the longline fleet operating in this area. From 2013 to 2014, we assessed the efficiency and practical applicability of four mitigation measures on artisanal demersal longliners targeting European hake (Merluccius merluccius) in the western Mediterranean: night setting, tori line, weighted lines and artificial baits. We performed fifty-two pairs of experimental (with the tested mitigation measure) and control settings (without any measure), and compared their effects on seabird interactions and fish catches. In addition, we estimated the longline sink rates and the seabird access area to baited hooks in different longline configurations. Night setting reduced bycatch risk without affecting target and non-commercial fish catches. The tori line may have reduced the bycatch risk by displacing bait attacks beyond the end of the line, but at this distance shearwaters could still access to the baits and the streamers did not deter birds under calm wind conditions. Weighted lines increased sink rate, but it resulted in only a minor reduction of the seabird access window to baited hooks and led to some operational problems during the setting. Artificial baits substantially reduced commercial catches. Moreover, the seabird access to the baited hooks was influenced by the longline configuration, the setting speed and the relative position to the floats and weights. So far, night setting stands out as the best mitigation measure for reducing bycatch levels without compromising target catches in demersal longliners. Ideally, these results should be confirmed in longliners targeting species other than European hake.

(140) Sex and age-biased mortality of three shearwaters species in longline fisheries of the Mediterranean. Marine Ecology Progress Series. 588:229-245.

Cortés, V., S.García-Barcelona & J.González-Solís (2018)

Hundreds of thousands of seabirds die annually as a result of being bycaught in longline fisheries, and these rates are unsustainable for many seabird populations worldwide. To understand effects at the population level, it is essential to assess sex- and age-biased mortalities, since uneven mortalities may exacerbate bycatch impacts. In the Mediterranean, bycatch is the main cause of population declines for the 3 endemic shearwater species (Scopoli’s Calonectris diomedea, Balearic Puffinus mauretanicus and Mediterranean shearwater P. yelkouan), but little is known about population biases of the birds caught in longliners. From 2003 to 2015, we collected 639 shearwater carcasses from Spanish longliners operating in the north-western Mediterranean, determined their age and sex and examined their spatial and temporal patterns and the origin of ringed birds. Most shearwaters caught in longliners were adults, but the proportion of immatures and subadults increased in the late breeding period. Adult Scopoli’s shearwaters were mostly caught around the breeding colonies. In contrast, all Puffinus birds were caught on the Iberian shelf. Catches of Scopoli’s shearwaters were male-biased, particularly during the pre-laying period and close to the breeding colonies. Catches of Puffinus shearwaters were also male-biased during the pre-laying period, but adult catches were female-biased during chick-rearing. Ring recoveries revealed that most birds were ringed in the nearby Balearic Islands, but some Scopoli’s shearwaters ringed in France and Italy were also caught during their migration, indicating that the impacts of the Spanish longliners extend well beyond the Spanish colonies. The adult-biased and sex-biased mortality found in this study may aggravate bycatch impacts on populations and highlights the urgent need for conservation action.

Reproductive timing in many taxa plays a key role in determining breeding productivity1, and is often sensitive to climatic conditions2. Current climate change may alter the timing of breeding at different rates across trophic levels, potentially resulting in temporal mismatch between the resource requirements of predators and their prey3. This is of particular concern for higher-trophic-level organisms, whose longer generation times confer a lower rate of evolutionary rescue than primary producers or consumers4. However, the disconnection between studies of ecological change in marine systems makes it difficult to detect general changes in the timing of reproduction5. Here, we use a comprehensive meta-analysis of 209 phenological time series from 145 breeding populations to show that, on average, seabird populations worldwide have not adjusted their breeding seasons over time (-0.020?days?yr-1) or in response to sea surface temperature (SST) (-0.272?days?°C-1) between 1952 and 2015. However, marked between-year variation in timing observed in resident species and some Pelecaniformes and Suliformes (cormorants, gannets and boobies) may imply that timing, in some cases, is affected by unmeasured environmental conditions. This limited temperature-mediated plasticity of reproductive timing in seabirds potentially makes these top predators highly vulnerable to future mismatch with lower-trophic-level resources.

Given the major ongoing influence of environmental change on the oceans, there is a need to understand and predict the future distributions of marine species in order to plan appropriate mitigation to conserve vulnerable species and ecosystems. In this study we use tracking data from seven large seabird species of the Southern Ocean (black-browed albatross Thalassarche melanophris, grey-headed albatross T. chrysostoma, northern giant petrel Macronectes halli, southern giant petrel M. giganteus, Tristan albatross Diomedea dabbenena, wandering albatross D. exulans and white-chinned petrel Procellaria aequinoctialis, and on fishing effort in two types of fisheries (characterised by low or high-bycatch rates), to model the associations with environmental variables (bathymetry, chlorophyll-a concentration, sea surface temperature and wind speed) through ensemble species distribution models. We then projected these distributions according to four climate change scenarios built by the Intergovernmental Panel for Climate Change for 2050 and 2100. The resulting projections were consistent across scenarios, indicating that there is a strong likelihood of poleward shifts in distribution of seabirds, and several range contractions (resulting from a shift in the northern, but no change in the southern limit of the range in four species). Current trends for southerly shifts in fisheries distributions are also set to continue under these climate change scenarios at least until 2100; some of these may reflect habitat loss for target species that are already over-fished. It is of particular concern that a shift in the distribution of several highly threatened seabird species would increase their overlap with fisheries where there is a high-bycatch risk. Under such scenarios, the associated shifts in distribution of seabirds and increases in bycatch risk will require much-improved fisheries management in these sensitive areas to minimise impacts on populations in decline.

(137) Year-round movements of a small seabird and oceanic isoscapes in the tropical Atlantic. Marine Ecology Progress Series 579:169-183.

Zajková, Z., T.Militão & J.González-Solís (2017)

Despite the proliferation of seabird tracking studies, there is a relative paucity of studies on small tropical seabirds. We present for the first time the distribution and movements of the little-known Boyd’s shearwater Puffinus boydi, a Procellariiform endemic to the Cape Verde Islands. We tracked 28 birds from 2 breeding sites (Ilhéu Raso and Ilhéu de Cima) with geolocator loggers from 2007 to 2012. We also analysed stable isotopes of carbon and nitrogen in the 1st primary (P1), the 6th rectrice (R6) and the 1st (S1) and 8th (S8) secondary feathers to reveal moulting pattern and oceanic isotopic gradients. Birds migrated on average 1452 km westward, to the central Atlantic Ocean (5 to 15°N, 30 to 40°W), where they stayed on average 114 d, from May to August. Boyd’s shearwaters exploited oceanic waters year-round and showed d13C values similar to other oceanic seabird species and d15N values indicating the lowest known trophic level among all central Atlantic seabirds. Isotope values in flight feathers suggest most animals moult their P1 and R6 around the breeding ground, whereas all birds moult S1 and S8 at the non-breeding quarters. Correlations of d13C and d15N values from S8 with the longitude of the non-breeding area indicate the existence of large-scale isotopic gradients matching those known at baseline levels. Combining geolocator tracking and stable isotope analyses in feathers not only allowed us to describe in detail the annual life cycle and distribution of the species, but also the oceanic isotopic gradients in the tropical Atlantic.

With increasing pressure on the oceans from environmental change, there has been a global call for improved protection of marine ecosystems through the implementation of marine protected areas (MPAs). Here, we used species distribution modelling (SDM) of tracking data from 14 seabird species to identify key marine areas in the southwest Atlantic Ocean, valuing areas based on seabird species occurrence, seasonality and extinction risk. We also compared overlaps between the outputs generated by the SDM and layers representing important human threats (fishing intensity, ship density, plastic and oil pollution, ocean acidification), and calculated loss in conservation value using fishing and ship density as cost layers. The key marine areas were located on the southern Patagonian Shelf, overlapping extensively with areas of high fishing activity, and did not change seasonally, while seasonal areas were located off south and southeast Brazil and overlapped with areas of high plastic pollution and ocean acidification. Non-seasonal key areas were located off northeast Brazil on an area of high biodiversity, and with relatively low human impacts. We found support for the use of seasonal areas depending on the seabird assemblage used, because there was a loss in conservation value for the seasonal compared to the non-seasonal approach when using ‘cost’ layers. Our approach, accounting for seasonal changes in seabird assemblages and their risk of extinction, identified additional candidate areas for incorporation in the network of pelagic MPAs.

(135) Causes and consequences of individual variability and specialization in foraging and migration strategies of seabirds. Marine Ecology Progress Series 578:117-150.

Phillips, R.A., S.Lewis, J.González-Solís & F.Daunt (2017)

Technological advances in recent years have seen an explosion of tracking and stable isotope studies of seabirds, often involving repeated measures from the same individuals. This wealth of new information has allowed the examination of the extensive variation among and within individuals in foraging and migration strategies (movements, habitat use, feeding behaviour, trophic status, etc.) in unprecedented detail. Variation is underpinned by key life-history or state variables such as sex, age, breeding stage and residual differences among individuals (termed ‘individual specialization’). This variation has major implications for our understanding of seabird ecology, because it affects the use of resources, level of intra-specific competition and niche partitioning. In addition, it determines the responses of individuals and populations to the environment and the susceptibility to major anthropogenic threats. Here we review the effects of season (breeding vs. nonbreeding periods), breeding stage, breeding status, age, sex and individual specialization on foraging and migration strategies, as well as the consequences for population dynamics and conservation.

Shearwaters deprived of their olfactory sense before being displaced to distant sites have impaired homing ability but it is unknown what the role of olfaction is when birds navigate freely without their sense of smell. Furthermore, treatments used to induce anosmia and to disrupt magneto-reception in displacement experiments might influence non-specific factors not directly related to navigation and, as a consequence, the results of displacement experiments can have multiple interpretations. To address this, we GPS-tracked the free-ranging foraging trips of incubating Scopoli’s shearwaters within the Mediterranean Sea. As in previous experiments, shearwaters were either made anosmic with 4% zinc sulphate solution, magnetically impaired by attachment of a strong neodymium magnet or were controls. We found that birds from all three treatments embarked on foraging trips, had indistinguishable at-sea schedules of behaviour and returned to the colony having gained mass. However, we found that in the pelagic return stage of their foraging trips, anosmic birds were not oriented towards the colony though coastal navigation was unaffected. These results support the case for zinc sulphate having a specific effect on the navigational ability of shearwaters and thus the view that seabirds consult an olfactory map to guide them across seascapes.

(133) It is the time for oceanic seabirds: tracking year-round distribution of gadfly petrels across the Atlantic Ocean. Diversity and Distributions 23: 794-805.

Anthropogenic activities alter and constrain the structure of marine ecosystems with implications for wide-ranging marine vertebrates. In spite of the environmental importance of vast oceanic ecosystems, most conservation efforts mainly focus on neritic areas. To identify relevant oceanic areas for conservation, we assessed the year-round spatial distribution and spatio-temporal overlap of eight truly oceanic seabird species of gadfly petrels (Pterodroma spp.) inhabiting the Atlantic Ocean. Using tracking data (mostly from geolocators), we examined year-round distributions, the timing of life-cycle events, and marine habitat overlap of eight gadfly petrel species that breed in the Atlantic Ocean. We compiled 125 year-round tracks. Movement strategies ranged from non-migratory to long-distance migrant species and from species sharing a common non-breeding area to species dispersing among multiple non-breeding sites. Gadfly petrels occurred throughout the Atlantic Ocean but tended to concentrate in subtropical regions. During the boreal summer, up to three species overlapped spatio-temporally over a large area around the Azores archipelago. During the austral summer, up to four species coincided in a core area in subtropical waters around Cape Verde, and three species shared habitat over two distinct areas off Brazil. The petrels used many national Exclusive Economic Zones, although they also exploited offshore international waters. Tracking movements of highly mobile vertebrates such as gadfly petrels can provide a powerful tool to evaluate and assess the potential need for and location of protected oceanic areas. As more multispecies, year-round data sets are collected from wide-ranging vertebrates, researchers and managers will have greater insight into the location of biodiversity hotspots. These can subsequently inform and guide marine spatial planning efforts that account for both conservation and sustainable use of resources such as commercial fisheries.

The Convention on Biological Diversity aspires to designate 10% of the global oceans as Marine Protected Areas (MPAs), but so far, few MPAs protect pelagic species in the high seas. Transparent scientific approaches are needed to ensure that these encompass areas with high biodiversity value. Here we used the distribution of all globally threatened seabirds breeding in a centrally located archipelago (Tristan da Cunha) to provide guidance on where MPAs could be established in the South Atlantic Ocean. We combined year-round tracking data from six species, and used the systematic conservation-planning tool, ‘Zonation’, to delineate areas that would protect the largest proportion of each population. The areas used most intensively varied among species and seasons. Combining the sites used by all six species suggested that the most important areas of the South Atlantic are located south of South Africa, around the central South Atlantic between 30°S and 55°S, and near South America. We estimated that the longline fishing effort in these intensively used areas is around 11 million hooks on average each year, highlighting the need for improved monitoring of seabird bycatch rates and the enforcement of compliance with bird bycatch mitigation requirements by fisheries. There was no overlap between the identified areas and any of the existing MPAs in the South Atlantic. The conservation of these highly mobile, pelagic species cannot be achieved by single countries, but requires a multi-national approach at an ocean-basin scale, such as an agreement for the conservation of biodiversity beyond national jurisdiction under the United Nation Convention on the Law of the Sea.

(131) Population size, breeding biology and on land threats of Fea's petrel (Pterodroma feae) in Fogo Island, Cape Verde. PLoS ONE 12: e0174803.

Cape Verde petrel (Pterodroma feae) is currently considered near threatened, but little is known about its population size, breeding biology and on land threats, jeopardizing its management and conservation. To improve this situation, we captured, marked and recaptured (CMR) birds using mist-nets over 10 years; measured and sexed them; monitored up to 14 burrows, deployed GPS devices on breeders and analyzed activity data of geolocators retrieved from breeders in Fogo (Cape Verde). We set cat traps over the colony and investigated their domestic/feral origin by marking domestic cats from a nearby village with transponders, by deploying GPS devices on domestic cats and by performing stable isotope analyses of fur of the trapped and domestic cats. The population of Fogo was estimated to be 293 birds, including immatures (95% CI: 233–254, CMR modelling). Based on geolocator activity data and nest monitoring we determined the breeding phenology of this species and we found biometric differences between sexes. While monitoring breeding performance, we verified a still ongoing cat predation and human harvesting. Overall, data gathered from trapped cats without transponder, cats GPS trips and the distinct isotopic values between domestic and trapped cats suggest cats visiting the colony are of feral origin. GPS tracks from breeders showed birds left and returned to the colony using the sector NE of the islands, where high level of public lights should be avoided specially during the fledging period. Main threats for the Cape Verde petrel in the remaining breeding islands are currently unknown but likely to be similar to Fogo, calling for an urgent assessment of population trends and the control of main threats in all Cape Verde Islands and uplisting its conservation status.

Bycatch mortality in longline fisheries is considered the main threat at sea for numerous seabird species. These incidental catches occur worldwide, but mortality levels are mainly determined by the specific traits of the fishery operating in each area and the feeding behaviour and local abundance of seabirds. In the Mediterranean, demersal artisanal longliners are known to catch several seabirds, but bycatch rates and the main factors influencing both the probability and the level of seabird bycatch are poorly known. From 2011 to 2015 we studied seabird interactions with longline vessels from onboard observations across the Balearic Sea. In addition, we recorded bird catches reported by fishermen. We found an average overall bycatch rate of 0.58 birds per 1,000 hooks (0.13 – 1.37, 95%CI), which would imply a conservative estimate ranging from 274 to 2,198 seabirds caught every year on demersal longliners in the study area. The most affected species were the three endemic and threatened shearwaters of the Mediterranean (Scopoli’s Calonectris diomedea, Balearic Puffinus mauretanicus and Yelkouan shearwaters P. yelkouan), likely due to their highly aggregative behaviour and diving capabilities. Overall, the main factors influencing bycatch risk were the season (breeding stage) and the time of day (higher risk at sunrise). Other influential factors were the bait type, the wind conditions, the distance between weights, the proximity to the breeding colony and the number of hooks. Both observations onboard and information from fishermen showed a paucity of “massive” bycatch events, particularly of the two smaller and most threatened shearwaters (Puffinus spp.). This study shows that mortality caused by demersal longliners is high and may be jeopardizing the viability of the shearwater populations. Therefore, the search and implementation of mitigation measures is urgently required.

Phylogenetic relationships among species can provide insight into how new species arise. For example, careful consideration of both the phylogenetic and geographic distributions of species in a group can reveal the geographic models of speciation within the group. One such model, sympatric speciation, may be more common than previously thought. The Hydrobatinae (Aves: Procellariformes) is a diverse subfamily of Northern Hemisphere storm-petrels for which the taxonomy is unclear. Previous studies showed that Hydrobates (formally Oceanodroma) castro breeding in the Azores during the cool season is sister species to H. monteiroi, a hot season breeder at the same locations, which suggests sympatric speciation by allochrony. To test whether other species within the subfamily arose via sympatric speciation by allochrony, we sequenced the cytochrome b gene and five nuclear introns to estimate a phylogenetic tree using multispecies coalescent methods, and to test whether species breeding in the same geographic area are monophyletic. We found that speciation within the Hydrobatinae appears to have followed several geographic modes of divergence. Sympatric seasonal species in Japan likely did not arise through sympatric speciation, but allochrony may have played a role in the divergence of H. matsudairae, a cool season breeder, and H. monorhis, a hot season breeder. No other potential cases of sympatric speciation were discovered within the subfamily. Despite breeding in the same geographic area, hydrobatine storm-petrels breeding in Baja California (H. microsoma and H. melania) are each sister to a species breeding off the coast of Peru (H. tethys and H. markhami, respectively). In fact, antitropical sister species appear to have diverged at multiple times, suggesting allochronic divergence might be common. In addition, allopatry has likely played a role in divergence of H. furcata, a north Pacific breeder, and H. pelagius, a north Atlantic breeder. This study demonstrates that a variety of mechanisms of divergence have played a role in generating the diversity of the Hydrobatinae and supports the current taxonomy of the subfamily.

Fisheries provide an abundant and predictable food source for many pelagic seabirds through discards, but also pose a major threat to them through bycatch, threatening their populations worldwide. The reform of the European Common Fisheries Policy (CFP), which intends to ban discards through the landing obligation of all catches, may force seabirds to seek alternative food sources, such as baited hooks from longlines, increasing bycatch rates. To test this hypothesis we performed a combined analysis of seabird-fishery interactions using as a model Scopoli’s shearwaters Calonectris diomedea in the Mediterranean. Tracking data showed that the probability of shearwaters attending longliners increased exponentially with a decreasing density of trawlers. On-board observations and mortality events corroborated this result: the probability of birds attending longliners increased 4% per each trawler leaving the longliner proximity and bird mortality increased tenfold when trawlers were not operating. Therefore, the implementation of the landing obligation in EU waters will likely cause a substantial increase in bycatch rates in longliners, at least in the short-term, due to birds switching from trawlers to longliners. Thus the implementation of the landing obligation must be carefully monitored and counterbalanced with an urgent implementation of bycatch mitigation measures in the longlinefleet.

Host specific adaptations in parasites can lead to the divergence of conspecific populations. However, this divergence can be difficult to measure because morphological changes may not be expressed or because obvious changes may simply reflect phenotypic plasticity. Combining both genetic and phenotypic information can enable a better understanding of the divergence process and help identify the underlying selective forces, particularly in closely-related species groups. Here, we link genetic and morphometric data to understand divergence patterns within the Ornithodoros (Carios) capensis complex, a group of soft ticks (Argasidae) exploiting colonial seabirds across the globe. Species designations in this complex were historically based on larval morphology and geographic location. However, recent work has suggested that divergence within the group may be at least partially linked to host specificity. We therefore first examined population genetic structure of ticks in relation to host use and geography. These analyses revealedstrong structure in relation to host use, both when populations were sympatric and widely allopatric, with a secondary effect of geography.They also demonstrated the presence of several novel and presumably undescribed species exploiting these seabird hosts. We then used geometric morphometrics (landmark and outline analyses) to test whether host-associated genetic divergence is always accompanied by the same phenotypic changes. We found that morphological variation (size and shape) correlated well with genetic structure; tick size and shape varied strongly in relation to host type, and weakly with geography. These results support the hypothesis that speciation in this tick group has been more strongly shaped by host use than by geographic barriers per se. The revealed phenetic patterns now require detailed investigation to link them with host-specific selective forces.

(126) Common Terns on the east Atlantic flyway: temporal-spatial distribution during the non-breeding period. Journal of Ornithology 157:927-940.

We studied the temporal–spatial distribution of Common Terns Sterna hirundo along the East Atlantic Flyway. In 2009 and 2010 experienced adults from a colony on the German North Sea coast were tagged with geolocators recording light intensity and saltwater contact. Main objectives were the inter-individual temporal–spatial variation of migration routes and wintering areas, wintering site fidelity, and time spent at sea across the annual cycle. Geolocators had no effects on various traits of breeders, but their reproductive output suffered from egg breakage. This can be avoided by artificially incubating the eggs. Twelve routes of nine individuals were tracked. Transponder readings at the breeding site showed that birds left the colony 4 weeks before starting autumn migration. In spring and autumn, Common Terns stopped over around the Canary Islands. Main wintering distribution was the upwelling seas alongside the West African coast and similar between years, but different among individuals. Three females wintered further north and more offshore than six males. Pair mates wintered at different locations. Spring migration was longer (56 ± 8 days) than autumn migration (37 ± 17 days). During both migration and wintering the terns spent more time on salt water than during breeding and post-breeding. In most individuals saltwater contact was higher during the day than at night, reduced at sunrise and sunset likely due to foraging, and peaked about noon possibly related to resting or thermoregulation. Detailed ecological and behavioral studies of common terns during wintering are needed to clarify the results based on geolocators.

Upwelling regions are highly productive habitats targeted by wide-ranging marine predators and industrial fisheries. In this study, we track the migratory movements of eight seabird species from across the Atlantic; quantify overlap with the Canary Current Large Marine Ecosystem (CCLME) and determine the habitat characteristics that drive this association. Our results indicate the CCLME is a biodiversity hotspot for migratory seabirds; all tracked species and more than 70% of individuals used this upwelling region. Relative species richness peaked in areas where sea surface temperature averaged between 15 and 20°C, and correlated positively with chlorophyll a, revealing the optimum conditions driving bottom-up trophic effects for seabirds. Marine vertebrates are not confined by international boundaries, making conservation challenging. However, by linking diversity to ocean productivity, our research reveals the significance of the CCLME for seabird populations from across the Atlantic, making it a priority for conservation action.

Ardeola is the scientific journal of the Spanish Ornithological Society. We analyse historical changes in citation, topics and foreign authorship of articles published in Ardeola from its first publication in 1954 up to last year, 2015, to test to what extent the persistence of the journal during the last 61 years has been due to support of authors, Society members, readers, editors or the whole ornithological community. Analyses were done within the context of the Red Queen game played by scientific journals competing for the best and more cited articles. The impact factor of Ardeola has increased from 1985 onwards both in absolute and relative terms. Thematic changes have followed trends of the general ornithological literature, without the journal specialising in particular topics or geographical regions. Foreign authorship decreased from 1954 up to the end of the 20th century, subsequently increasing again, a trend fuelled by coverage by Current Contents and the JCR, the establishment of English as the language of publication and recent Internet access through the BioOne platform. Ardeola is a traditional scientific journal, backed by a scientific society, whose future will be guaranteed by a reputation for rigour and quality sought by authors, reviewers and editors, supported by the members of the Spanish Ornithological Society and retaining its original objective: ‘to be a journal at the level of the best…, looking for a strong collaboration with foreign authors to promote the benefit of the Ornithology’.

Studies on Persistent Organic Pollutants (POPs) in Antarctic wildlife are scarce, and usually limited to a single locality. As a result, wildlife exposure to POPs across the Southern Ocean is poorly understood. In this study, we report the differential exposure of the major southern ocean scavengers, the giant petrels, to POPs across a wide latitudinal gradient. Selected POPs (PCBs, HCB, DDTs, PBDEs) and related compounds, such as Dechlorane Plus (DP), were analyzed in plasma of southern giant petrels (Macronectes giganteus) breeding on Livingston (62°S 61°W, Antarctica), Marion (46°S 37°E, sub-Antarctic), and Gough (40°S 10°W, cool temperate) islands. Northern giant petrels (Macronectes halli) from Marion Island were also studied. Stable isotope ratios of C and N (d(13)C and d(15)N) were used as dietary tracers of the marine habitat and trophic level, respectively. Breeding locality was a major factor explaining petrel exposure to POPs compared with species and sex. Significant relationships between d(13)C values and POP burdens, at both inter- and intra-population levels, support latitudinal variations in feeding grounds as a key factor in explaining petrel pollutant burdens. Overall, pollutant levels in giant petrels decreased significantly with latitude, but the relative abundance (%) of the more volatile POPs increased towards Antarctica. DP was found at negligible levels compared with legacy POPs in Antarctic seabirds. Spatial POP patterns found in giant petrels match those predicted by global distribution models, and reinforce the hypothesis of atmospheric long-range transport as the main source of POPs in Antarctica. Our results confirm that wildlife movements out of the polar region markedly increase their exposure to POPs. Therefore, strategies for Antarctic wildlife conservation should consider spatial heterogeneity in exposure to marine pollution. Of particular relevance is the need to clarify the exposure of Antarctic predators to emerging contaminants that are not yet globally regulated.

The conservation status and taxonomy of the three gadfly petrels that breed in Macaronesia is still discussed partly due to the scarce information on their spatial ecology. Using geolocator and capture-mark-recapture data, we examined phenology, natal philopatry and breeding-site fidelity, year-round distribution, habitat usage and at-sea activity of the three closely-related gadfly petrels that breed in Macaronesia: Zino’s petrel Pterodroma madeira, Desertas petrel P. deserta and Cape Verde petrel P. feae. All P. feae remained around the breeding area during their non-breeding season, whereas P. madeira and P. deserta dispersed far from their colony, migrating either to the Cape Verde region, further south to equatorial waters in the central Atlantic, or to the Brazil Current. The three taxa displayed a clear allochrony in timing of breeding. Habitat modelling and at-sea activity patterns highlighted similar environmental preferences and foraging behaviours of the three taxa. Finally, no chick or adult was recaptured away from its natal site and survival estimates were relatively high at all study sites, indicating strong philopatry and breeding-site fidelity for the three taxa. The combination of high philopatry, marked breeding asynchrony and substantial spatio-temporal segregation of their year-round distribution suggest very limited gene flow among the three taxa.

Understanding the spread of Toxoplasma gondii (T. gondii) in wild birds, particularly in those with opportunistic feeding behavior, is of interest for elucidating the epidemiological involvement of these birds in the maintenance and dissemination of the parasite. Overall, from 2009 to 2011, we collected sera from 525 seagull chicks (Yellow-legged gull (Larus michahellis) and Audouin's gull (L. audouinii)) from 6 breeding colonies in Spain and tested them using the modified agglutination test (MAT) for the presence of antibodies against T. gondii. Chick age was estimated from bill length. Main food source of seagull chicks was evaluated using stable isotope analyses from growing scapular feathers. Overall T. gondii seroprevalence was 21.0% (IC95% 17.5-24.4). A generalized linear mixed-effects model indicated that year (2009) and food source (freshwater) were risk factors associated to the individual risk of infection by T. gondii, while age (days) was close to significance. Freshwater food origin was related to the highest seroprevalence levels, followed by marine origin, supporting freshwater and sewages as important routes of dispersion of T. gondii. Year differences could indicate fluctuating rates of exposure of seagull chicks to T. gondii. Age ranged from 4 to 30 days and seropositivity tended to increase with age (P = 0.07), supporting that seropositivity is related to T. gondii infection rather than to maternal transfer of antibodies, which in gulls is known to sharply decrease with chick age. This study is the first to report T. gondii antibodies in Yellow-legged and Audouin's gulls, thereby extending the range of intermediate hosts for this parasite and underscoring the complexity of its epidemiology.

Chemical signals may be the basis of interspecific recognition and speciation in many animals. To test whether a chemical label allowing recognition between closely related species exists in seabirds, we investigated two closely related taxa breeding sympatrically at some localities: Cory's and Scopoli's shearwaters. Procellariiform seabirds are ideal for this study because they have a well-developed olfactory system and unequalled associated capabilities among birds. We analysed and compared the relative volatile compounds composition of the uropygial gland secretions of Cory's and Scopoli's shearwaters. As the volatile components from uropygial secretions might also provide some critical eco-chemical information about population origin and sex, we also examined variations in the volatile compounds between populations and sexes in Cory's shearwater. The chemical data were obtained using gas chromatography–mass spectrometry techniques looking for the presence of these three particular labels: species, population and gender. We found diagnostic differences in uropygial secretions between the two species of shearwaters and smaller but significant variation between populations of Cory's shearwater in the Atlantic. No significant differences were observed between males and females. Individuals might thus use the chemical variation between species to recognize and mate with conspecifics, especially at localities where both species breed sympatrically. Geographical variation in chemical composition of uropygial secretions suggests that selective forces might vary according with locations, and might provide a key in the species recognition. Further behavioural bioassays are needed to determine whether or not these species labels are signals allowing reproductive isolation between these two taxa. Finally, one of the aims of our study was to test easier methods than freezing for storing uropygial gland secretions in the field. We describe here a new possibility for the storage of uropygial secretion samples at ambient temperature in the field, providing an alternative, simple protocols for the sampling of avian chemosignals. This article is protected by copyright. All rights reserved.

(118) The State of Mediterranean and Black Sea Fisheries 2016. FAP-GFCM. 145pp.

Fishing has a tremendous cultural and economic importance in the Mediterranean and the Black Sea, providing an important source of food and livelihoods for riparian countries and sustaining the traditions and the way of life of many coastal communities. However, the fisheries sector faces many challenges, including the increasing demand for fish and the potential negative effects of pollution, habitat degradation, the appearance of alien species, overfishing, and climate-driven changes on marine ecosystems. This first edition of the GFCM report on the State of Mediterranean and Black Sea fisheries includes a comprehensive analysis of the current state and past trends of fisheries in the GFCM area of application. This analysis has been carried out by different GFCM scientific bodies, the GFCM Secretariat as well as independent experts, based on different sources of information, including official data submitted by GFCM members, national reports, questionnaires, etc. The report sheds light on various aspects of fisheries, such as the extent and composition of the fishing fleet, the production and socioeconomic characteristics of fisheries, the composition and volume of discards, the incidental catch of vulnerable species and the status of stocks. Current efforts deployed by GFCM towards the governance and management of fisheries, including small-scale fisheries are also discussed, with a particular focus on accomplishments and areas for future action. The report is intended to become a flagship GFCM publication for the regular dissemination of information related to fisheries in the Mediterranean and the Black Sea and should serve as a tool to support decision-making on fisheries issues of relevance to the GFCM area of application.

According to classic niche theory, species can coexist in heterogeneous environments by reducing interspecific competition via niche partitioning, e.g. trophic or spatial partitioning. However, support for the role of competition on niche partitioning remains controversial. Here, we tested for spatial and trophic partitioning in feather mites, a diverse and abundant group of arthropods. We focused on the two dominant mite species, Microspalax brevipes and Zachvatkinia ovata, inhabiting flight feathers of the Cory’s shearwater, Calonectris borealis.We performed mite counts across and within primary and tail feathers on free-living shearwaters breeding on an oceanic island (Gran Canaria, Canary Islands). We then inves-
tigated trophic relationships between the two mite species and the host using stable isotope analyses of carbon and nitrogen on mite tissues and potential host food sources. The distribution of the two mite species showed clear spatial segregation among feathers; M.brevipes showed high preference for the central wing primary feathers, whereas Z.ovata was restricted to the two outermost primaries. Morphological differences between M.brevipes and Z.ovata support an adaptive basis for the spatial segregation of the two mite species. However, the two mites overlap in some central primaries and statistical modeling showed that Z.ovata tends to outcompete M.brevipes. Isotopic analyses indicated similar isotopic values for the two mite species and a strong correlation in carbon signatures between mites inhabiting the same individual host suggesting that diet is mainly based on shared host-associated resources. Among the four candidate tissues examined (blood, feather remains, skin remains and preen gland oil), we conclude that the diet is most likely dominated by preen gland oil, while the contribution of exogenous material to mite diets is less marked. Our results indicate that ongoing competition for space and resources plays a central role in structuring feather mite communities. They also illustrate that symbiotic infracommunities are excellent model systems to study trophic ecology, and can improve our understanding of mechanisms of niche differentiation and species coexistence.

(116) The role of sardine as prey for pelagic predators in the western Mediterranean Sea assessed using stable isotopes and fatty acids. Marine Ecology Progress Series 531: 1-14.

FEATURE ARTICLE

Cardona, L., L.M.Iñigo, R.Mateo, J.González-Solís (2015)

This study combined the analysis of fatty
acids and stable isotopes of nitrogen and carbon to
test the hypothesis that the pelagic food web of the
south Catalan Sea has a wasp-waist structure supported
by sardines
Sardina
pilchardus.
If this hypo thesis
were
correct,
most predators
would be expected
to
have stable isotope ratios and fatty acid profiles
consistent
with those derived from
a sardine-based
diet.
However,
this was true
only for mackerel
Scomber
scomber,
blue butterfish
Stromateus
fiatola,
all seabirds
and oceanic loggerhead
turtles
Caretta
caretta.
The
values of the DHA/EPA
index of neritic loggerhead
turtles
and striped dolphins Stenella
caeru
leo
alba
were
also consistent with a sardine/
squid
diet, but
their
trophic
positions were
too high. On the other
hand,
the DHA/EPA
index of most predatory
fishes indicated
that anchovies Engraulis
encrasicolus
and/or
horse mackerel Trachurus trachurus were their main
prey. Nevertheless, some amounts of low trophic level
invertebrates were likely to be consumed by some
predatory fishes, because their trophic positions where
lower than expected from a fish-based diet only. The
heterogeneous distribution of phytoplankton groups
above and below the thermocline during the warm
season is hypothesized to be the primary reason for
this food web structure, although the strong reliance
of some seabirds on sardines is the likely consequence
of a massive consumption of discards from fishing
boats. In short, there is little evidence for a wasp-waist
structure based on sardine, which may explain why
the populations of predators fluctuate less than the
population of sardines.

Our current understanding of migratory strategies and the reasons for
their high variability along the phylogenetic tree remains relatively poor. Most
of the hypotheses relating to migration have been formulated for terrestrial
taxa; classically, oceanic migrations were considered as merely dispersive
because of the scarcity of observations in the open ocean. We describe for the
?rst time, the migration strategy of a small seabird, the Bulwer’s petrel (Bulwe-
ria bulwerii), and provide new insights into the ecology and evolution of long-
distance marine migrations. Using cutting-edge geolocators, we examined the year-round distri-
bution and at-sea activity patterns of adult Bulwer’s petrels sampled at ?ve
localities throughout the species’ breeding range in the Atlantic, within the Azores,
Salvages, Canary and Cape Verde archipelagos. We assessed the migratory
connectivity of the species and its habitat use at population and metapopulation
scales. Our results provide the ?rst evidence of an oriented leapfrog migration
in oceanic seabirds. Ecological niche models based on breeding-season
data effectively predicted that subtropical waters of the South Atlantic would
be the preferred habitat for the northern populations of Bulwer’s petrels during
the non-breeding season. Habitat modelling also highlighted similarities in distributions
between the breeding and non-breeding periods for the southern
populations. Data on at-sea activity patterns suggested that birds from the
northern and southern populations behave differently during the breeding season,
as well as in the northern and southern non-breeding ranges during the
non-breeding period. nThese results indicate that speci?c habitat preferences, presumably
related to differences in prey availability, explain the observed distributions
and hence the pattern of leapfrog migration described for Bulwer’s
petrel. Our study demonstrates the utility of integrating diverse tracking data
from multiple populations across international boundaries, and habitat modelling,
for identifying important areas common to many marine species in the
vast oceanic environments.

Two new species of the feather mite genus
Promegninia Gaud & Atyeo, 1967 (Avenzoariidae:
Bonnetellinae) are described from procellariids in the
northeasterm Atlantic Ocean: Promegninia bulweriae
n. sp. from the Bulwer’s petrel Bulweria bulwerii
(Jardine & Selby) and P. calonectris n. sp. from the
Cory’s shearwater Calonectris borealis (Cory) (Procellariiformes:
Procellariidae). Males of P bulweriae n. sp.
are most clearly distinguished from the other known
species in the genus by having short legs III extending
only to the level of lobar apices and short conical tarsi III
with lanceolate ventral seta w; females of this species are
characterised by the absence of additional sclerites at
postero-lateral angles of the prodorsal shield. Males of
P. calonectris differ from the other known species in
having bidentate terminal lamellae on the lobar apices
and the entire adanal shield; females of this species are
distinguished by having well-developed pygidial
shields and a hysteronotal shield encompassing the
bases of setae c2. Sequence data of the mitochondrial
cytochrome c oxidase subunit I gene fragment (COI) are
also obtained for the new species. An updated diagnosis
of Promegninia and a key to the known species are
provided.

(113) Comparing multiple criteria for species identification in two recently divergent seabirds. Plos One 9: e115650.

Militão, T., E.Gómez-Díaz, A.Kaliontzopoulou, J.González-Solís (2014)

Correct species identification is a crucial issue in systematics with key implications
for prioritising conservation effort. However, it can be particularly challenging in
recently diverged species due to their strong similarity and relatedness. In such
cases, species identification requires multiple and integrative approaches. In this
study we used multiple criteria, namely plumage colouration, biometric
measurements, geometric morphometrics, stable isotopes analysis (SIA) and
genetics (mtDNA), to identify the species of 107 bycatch birds from two closely
related seabird species, the Balearic (Puffinus mauretanicus) and Yelkouan (P.
yelkouan) shearwaters. Biometric measurements, stable isotopes and genetic data
produced two stable clusters of bycatch birds matching the two study species, as
indicated by reference birds of known origin. Geometric morphometrics was
excluded as a species identification criterion since the two clusters were not stable.
The combination of plumage colouration, linear biometrics, stable isotope and
genetic criteria was crucial to infer the species of 103 of the bycatch specimens. In
the present study, particularly SIA emerged as a powerful criterion for species
identification, but temporal stability of the isotopic values is critical for this purpose.
Indeed, we found some variability in stable isotope values over the years within
each species, but species differences explained most of the variance in the isotopic
data. Yet this result pinpoints the importance of examining sources of variability in
the isotopic data in a case-by-case basis prior to the cross-application of the SIA
approach to other species. Our findings illustrate how the integration of several
methodological approaches can help to correctly identify individuals from recently
diverged species, as each criterion measures different biological phenomena and
species divergence is not expressed simultaneously in all biological traits.

(112) Long antibody persistence and transgenerational immonoresponse in a long-lived vertebrate. American Naturalist 184:764-776.

Ramos, R., R.Garnier, J.González-Solís, T. Boulinier (2014)

Although little studied in natural populations, the persistence
of immunoglobulins may dramatically affect the dynamics
of immunity and the ecology and evolution of host-pathogen interactions
involving vertebrate hosts. By means of a multiple-year vaccination
design against Newcastle disease virus, we experimentally
addressed whether levels of speci?c antibodies can persist over several
years in females of a long-lived procellariiform seabird—Cory’s
shearwater—and whether maternal antibodies against that antigen
could persist over a long period in offspring several years after the
mother was exposed. We found that a single vaccination led to high
levels of antibodies for several years and that the females transmitted
antibodies to their offspring that persisted for several weeks after
hatching even 5 years after a single vaccination. The temporal persistence
of maternally transferred antibodies in nestlings was highly
dependent on the level at hatching. A second vaccination boosted
efficiently the level of antibodies in females and thus their transfer
to offspring. Overall, these results stress the need to consider the
temporal dynamics of immune responses if we are to understand
the evolutionary ecology of host-parasite interactions and trade-offs
between immunity and other life-history characteristics, in particular
in long-lived species. They also have strong implications for conservation
when vaccination may be used in natural populations facing
disease threats.

Offspring phenotypic variation has important
consequences for the future survival, reproductive success
and individual ?tness of nestlings, but the sex-speci?c
reproductive value and ?tness returns for parents of this
variation have only rarely been explored. We analysed
twelve phenotypic traits of nestling Common Terns (Sterna
hirundo) in relation to their sex, age and hatching date, and
explored whether sexual differences in these traits were
associated with the rearing environment and survival. In
addition, we studied sex-speci?c differences in offspring
phenotype related to parental factors, since the existence of
sexual dimorphism may re?ect differential parental
investment. Bill–head length and mediated immune
response varied in relation to offspring sex, with males
showing larger values than females. Immune response was
also affected by environmental (year), rearing (competition
over 14 days old) and parental (clutch size) factors. None of
the morphological, serological and immunological variables
were associated with chick mortality, but female
nestlings that did not survive had suffered longer periods of
within-brood competition. Although some parental traits
in?uenced offspring phenotype, sexual differences among
nestlings did not vary in relation to parental age, mass or
arrival date, which may imply that there is no great difference
in cost between raising sons and raising daughters, and thus no adjustment of parental effort in relation to nestling
sex. However, the evidence that immune response was
higher for sons in larger clutches that were raised with
siblings indicates a difference in the developmental strategies
for sons and daughters, which may be related to their
reproductive value. These results therefore provide evidence
of certain pathways for parental sex allocation that
may differentially affect sons and daughters.

In migratory birds, environmental conditions in both breeding and non-breeding
areas may affect adult survival rates and hence be significant drivers of demographic processes.
In seabirds, poor knowledge of their true distribution outside the breeding season, however, has
severely limited such studies. This study explored how annual adult survival rates of black-legged
kittiwakes Rissa tridactyla on Hornøya in the southern Barents Sea were related to temporal variation
in prey densities and climatic parameters in their breeding and non-breeding areas. We
used information on the kittiwakes’ spatiotemporal distribution in the non-breeding season
gained from year-round light-based tracking devices (geolocators) and satellite transmitters, and
kittiwake annual adult survival rates gained from a multistate capture-mark-recapture analysis of
a 22 yr time series of colour-ringed kittiwakes. In the post-breeding period, kittiwakes concentrated
in an area east of Svalbard, in the winter they stayed in the Grand Banks/Labrador Sea
area, and in the pre-breeding period they returned to the Barents Sea. We identified 2 possible
prey categories of importance for the survival of kittiwakes in these areas (sea butterflies Thecosomata
in
the
Grand
Banks/Labrador
Sea
area
in
winter
and
capelin
Mallotus
villosus
in the Barents
Sea
in
the
pre-breeding
season)
that
together
explained
52
% of the variation in adult survival
rates. Our results may have important implications for the conservation of kittiwakes, which are
declining globally, because other populations use the same areas. Since they are under the influence
of
major
anthropogenic
activities
including
fisheries,
international
shipping
and
the
offshore
oil and gas industry, both areas should be targeted for future management plans.

In recent years, a number of zoonotic flaviviruses have emerged worldwide, and wild birds serve as their major reservoirs.
Epidemiological surveys of bird populations at various geographical scales can clarify key aspects of the eco-epidemiology
of these viruses. In this study, we aimed at exploring the presence of flaviviruses in the western Mediterranean by sampling
breeding populations of the yellow-legged gull (Larus michahellis), a widely distributed, anthropophilic, and abundant
seabird species. For 3 years, we sampled eggs from 19 breeding colonies in Spain, France, Algeria, and Tunisia. First, ELISAs
were used to determine if the eggs contained antibodies against flaviviruses. Second, neutralization assays were used to
identify the specific flaviviruses present. Finally, for colonies in which ELISA-positive eggs had been found, chick serum
samples and potential vectors, culicid mosquitoes and soft ticks (Ornithodoros maritimus), were collected and analyzed
using serology and PCR, respectively. The prevalence of flavivirus-specific antibodies in eggs was highly spatially
heterogeneous. In northeastern Spain, on the Medes Islands and in the nearby village of L’Escala, 56% of eggs had
antibodies against the flavivirus envelope protein, but were negative for neutralizing antibodies against three common
flaviviruses: West Nile, Usutu, and tick-borne encephalitis viruses. Furthermore, little evidence of past flavivirus exposure was
obtained for the other colonies. A subset of the Ornithodoros ticks from Medes screened for flaviviral RNA tested positive for
a virus whose NS5 gene was 95% similar to that of Meaban virus, a flavivirus previously isolated from ticks of Larus
argentatus in western France. All ELISA-positive samples subsequently tested positive for Meaban virus neutralizing
antibodies. This study shows that gulls in the western Mediterranean Basin are exposed to a tick-borne Meaban-like virus,
which underscores the need of exploring the spatial and temporal distribution of this flavivirus as well as its potential
pathogenicity for animals and humans.

Plastic debris are often ingested by marine predators and can cause health disorders and even death. We present the first assessment of plastic ingestion in Mediterranean seabirds. We quantified and measured plastics accumulated in the stomach of 171 birds from 9 species accidentally caught by longliners in the western Mediterranean from 2003 to 2010. Scopoli's shearwaters (Calonectris diomedea) showed the highest occurrence (94%) and large numbers of small plastic particles per affected bird (on average N=15.3 ±24.4 and mass=23.4±49.6mg), followed by Yelkouan shearwaters (Puffinus yelkouan, 70%, N=7.0 ±7.9, 42.1±100.0mg), Balearic shearwaters (Puffinus mauretanicus, 70%, N=3.6 ±2.9, 5.5±9.7mg) and the rest of species (below 33%, N=2.7, 113.6±128.4mg). Plastic characteristics did not differ between sexes and were not related to the physical condition of the birds. Our results point out the three endemic and threatened shearwater species are particularly exposed to plastic accumulation.

Ancient DNA has revolutionized conservation genetic studies as it allows monitoring of the genetic variability of species through time and predicting future impacts. In this study we examined temporal changes in the genetic diversity of a threatened seabird, the Cory’s shearwater (Calonectris borealis). We analysed the mitochondrial DNA control region of ancient bone samples from the late-Holocene retrieved from the Canary archipelago (NE Atlantic) together with current DNA sequences representative of the entire breeding range of the species. Our results show high levels of ancient genetic diversity in the Canaries comparable to that of the extant population. Temporal haplotype networks further revealed rare but recurrent long-distance dispersals between ocean basins. EBSP demographic analyses reveal both regional and local population size expansion events, and this is in spite of the demographic decline experienced by the species over the last millennia. Overall our findings suggest that population connectivity of the species has acted as a buffer of genetic losses and illustrate the use of ancient DNA to uncover such cryptic genetic events.

(106) Meta-population feeding grounds of Cory’s shearwater in the subtropical Atlantic Ocean: implications for the definition of Marine Protected Areas based on tracking studies. Diversity and Distributions 19: 1284-1298

Many apical pelagic species use predictable habitats to forage, and thus their movements should signal biologically and ecologically significant areas of the marine ecosystem. Several countries are now engaged in the process to identify these areas based on animal tracking, but this is often limited to a few individuals from a single breeding population, which may result in a biased portrayal of the key marine habitats. Location: North East Atlantic Ocean. Along seven years, we tracked the foraging movements of Cory’s shearwaters (Calonectris diomedea) from multiple populations during the chick-rearing period using GPS and PTT devices. Results We obtained foraging trips from 174 shearwaters breeding on six important breeding colonies representative of the range occupied in the Macaronesian Archipelagos of Madeira, Selvagens and Canaries. Our results show that birds orient and move rapidly towards the closest neritic waters over the African continental shelf. Birds from different colonies show a substantial spatial segregation in their foraging grounds but consistently overlap in some specific foraging areas along the Canary Current characterized by their high productivity. By weighing the use of foraging grounds according to the size of each study population, we inferred the overall exploitation of such areas. Main conclusions Our meta-population approach provides a more comprehensive scenario of space use from both perspectives, the studied species and the Canary Current system, a crucial issue often neglected in conservation studies. Foraging grounds consistently used by multiple populations may not be adequately identified by tracking a single population, and therefore meta-population tracking studies reveals as a crucial tool to properly delineate key conservation areas and inform conservation planning in the marine ecosystem. Finally, we suggest pending work on the long-term stability and sustainability of identified foraging areas and propose that countries with geographical jurisdictions over the Canary Current area should work towards multilateral agreements to set management plans for this key marine ecosystem.

The energetic demands of animals change throughout their annual life cycle. In migrating birds, reproduction and migration are the two most energy demanding processes; the transition from one to the other require a number of physiological adjustments. When arriving to the breeding grounds, long-distance migratory birds need to recover from migration and prepare for reproduction. This process is crucial, since pre-breeding body condition has been established as a critical factor influencing reproductive success in several avian species. In the present study we examined the physiological condition of the Cory´s shearwater (Calonectris diomedea borealis, Cory 1881) after arrival from migration to a breeding colony, as well as its evolution throughout the pre-laying period. We weighed and took blood samples from 149 shearwaters newly arrived to their breeding colony. If found again after 10, 20, 30 or 40 days since their first capture, birds were resampled. We examined individual variation of biochemical parameters, body mass and blood oxygen transport capacity in both males and females. Our results indicate that birds were improving their condition after arrival from migration. Plasma metabolites revealed shearwaters were depositing fat and degrading muscular mass during post-migration and pre-laying, which suggest that shearwaters were remodeling their body composition in order to have more space for lipid reserves. Females started fat deposition earlier than males, probably to cope with the lipid demands of egg formation. This study shows that biochemical blood parameters are good indicators of the ecophysiological changes experienced by post-migrating and pre-breeding birds.

Adaptive sex allocation has frequently been studied in sexually size dimorphic species, but far less is known about patterns of sex allocation in species without pronounced sexual size dimorphism. Parental optimal investment can be predicted under circumstances in which sons and daughters differ in costs and/or fitness returns. In Common Terns Sterna hirundo, previous studies suggest that sons are the more costly sex to produce and rear. We investigated whether hatching and fledging sex ratio and sex-specific chick mortality correlated with the ecological environment (laying date, clutch size, hatching order and year quality) and parental traits (condition, arrival date, experience and breeding success), over 7 consecutive years. Population-wide sex ratios and sex-specific mortality did not differ from parity, but clutch size, mass of the father, maternal breeding experience and to some extent year quality correlated with hatching sex ratio. The proportion of sons tended to increase in productive years and when the father was heavier, suggesting the possibility that females invest more in sons when the environmental and the partner conditions are good. The proportion of daughters increased with clutch size and maternal breeding experience, suggesting a decline in breeding performance or a resources balance solved by producing more of the cheaper sex. No clear patterns of sex-specific mortality were found, neither global nor related to parental traits. Our results suggest lines for future studies on adaptive sex allocation in sexually nearly monomorphic species.

Prospecting allows individuals to gather information on the local quality of potential future breeding sites. In a variable and heterogeneous environment, it plays a major role in breeding habitat selection and potentially helps individuals make optimal dispersal decisions. Although prospecting movements, involving visits to other breeding sites, have been observed in many species at relatively fine spatial scales, little is known about their occurrence at larger scales. Furthermore, the adaptive value of dispersal strategies in response to environmental changes remains poorly investigated. Here, our main objective is to highlight in what ways tracking devices could constitute powerful tools to study prospecting behaviour at various spatial scales. First, we stress the importance of considering prospecting movements involved in breeding habitat selection and we detail the type of data that can be collected. Then, we review the advantages and constraints associated with the use of tracking devices in this context, and we suggest new perspectives to investigate the behavioural strategies adopted by individuals during breeding habitat selection processes and dispersal decisions. The rapid development of new powerful electronic tools for tracking individual behaviour thus opens a wide range of opportunities.More specifically, itmay allow a more thorough understanding of the role of scale-dependent dispersal behaviour in population responses to environmental changes.

(102) The evolution of north east Atlantic gadfly petrels using statistical phylogeography. Molecular Ecology 22: 495-507.

Macaronesia (north-east Atlantic archipelagos) has been host to complex patterns of colonization and differentiation in many groups of organisms including seabirds such as gadfly petrels (genus Pterodroma). Considering the subspecies of widely distributed soft-plumaged petrel for many years, the taxonomic status of the three gadfly petrel taxa breeding in Macaronesia is not yet settled, some authors advocating the presence of three, two or one species. These birds have already been the subject of genetic studies with only one mtDNA gene and relatively modest sample sizes. In this study, using a total of five genes (two mitochondrial genes and three nuclear introns), we investigated the population and phylogeographical histories of petrel populations breeding on Madeira and Cape Verde archipelagos. Despite confirming complete lineage sorting with mtDNA, analyses with nucDNA failed to reveal any population structuring and Isolation with Migration analysis revealed the absence of gene flow during the differentiation process of these populations. It appears that the three populations diverged in the late Pleistocene in the last 150 000 years, that is 10 times more recently than previous estimates based solely on one mtDNA gene. Finally, our results suggest that the Madeira petrel population is ancestral rather than that from Cape Verde. This study strongly advocates the use of nuclear loci in addition to mtDNA in demographical and phylogeographical history studies.

(101) Serological and virological surveys of the influenza A virus in Antarctic and sub-Antarctic penguins. Antarctic Science 25:339-344.

To evaluate the avian influenza virus (AIV) circulation in Antarctic and sub-Antarctic penguins we carried out a serosurvey on six species from Livingston, Marion and Gough islands. Seropositivity against AIV was performed on serum samples using a competitive enzyme-linked immunosorbent assay and haemagglutination and neuraminidase inhibition assays. Some oropharyngeal and cloacal swabs were also assayed to detect influenza virus genomes by real time reverse transcription-polymerase chain reaction. Overall, 12.1% (n5140) penguins were seropositive to AIV. By species, we detected 5% (n519) and 11% (n518) seroprevalence in sub-Antarctic rockhopper penguins Eudyptes spp. from Gough and Marion islands, respectively, 42% (n533) seroprevalence in macaroni penguins Eudyptes chysolophus, but no positives in the three other species, gentoo Pygoscelis papua (n525) and chinstrap penguins P. antarctica (n516), from Livingston Island and king penguins Aptenodytes patagonicus (n527) from Marion Island While sero positivity reflected previous exposure to the AIV, the influenza genome was not detected. Our results indicate that AIV strains have circulated in penguin species in the sub-Antarctic region, but further studies are necessary to determine the precise role that such penguin species play in AIV epidemiology and if this circulation is species (or genus) specific.

Aim: Intraspecific variability in the migratory movements of seabirds is being revealed far more complex than hitherto recognized, and our lack of understanding undermines their effective protection. Our aim is to test whether the isotopic values of a single feather of two threatened seabirds, the Mediterranean (Puffinus yelkouan) and the Balearic (Puffinus mauretanicus) shearwaters allow the geographic assignment of birds to their non-breeding areas. Location These two species are known to use three main non-breeding areas: the NE Atlantic Ocean, the W Mediterranean and the Aegean-Black seas. Methods: We clustered in three groups the d13C and d15N values of the first primary feather (P1), inferred to be grown during the non-breeding period, of 34 Mediterranean and 56 Balearic shearwaters accidentally caught by longliners. To link the isotopic values of P1 to its corresponding non-breeding area, we performed a discriminant function analysis (DFA) based on the three clusters and applied this function to feathers of known origin: P1 from seven Mediterranean shearwaters from Hye`res Archipelago (France) tracked with geolocators and body feathers from six chicks from Balearic shearwaters. To link the moulting patterns to the areas where the feathers were grown, we applied theDFA to a sequence of primary feathers of eight Balearic and five Mediterranean shearwaters (caught by longliners). Results: Isotopic and tracking data indicate that none of the Mediterranean shearwaters migrated to the Atlantic. The cluster and discriminant function analyses revealed that 8% of Balearic and 54% of Mediterranean shearwater moulted P1 in the Mediterranean Sea. Migratory movements were reflected in the changing isotopic values of the primary sequences. Main conclusions: Stable isotope analyses (SIA) are a powerful approach to reveal the intraspecific variability in the migratory patterns of seabirds that usedistinct isotopic areas over their annual cycle. The assignment of birds to their non-breeding areas by means of SIA is a simple and effective tool that can help to evaluate the impact of human activities in remote areas not only at population but also at individual level, which is an essential knowledge for the management and conservation of threatened species.

Data about breeding populations of birds in the Antarctica are rare and fragmented. Thus, information about the status of the breeding populations of Antarctic birds is crucial given the current scenario of climate change, which is particularly acute in Antarctica. This paper presents new information about the populations of the Antarctic tern Sterna vittata, the kelp gull Larus dominicanus, the southern giant petrel Macronectes giganteus, the Antarctic skua Catharacta antarctica lonnbergi, the chinstrap penguin Pygoscelis antarctica and the gentoo penguin Pygoscelis papua on Byers Peninsula (Livingston Island, South Shetland Islands). We used line transects counts to estimate both densities and numbers of nests of the different species. We estimate that there are 398.96 birds km-2 of southern giant petrels (2793 individuals), 62.4 birds km-2 of Antarctic tern (3746 individuals) and 269.1 birds km-2 of kelp gull (1884 individuals). Furthermore, we found 15 nests of Antarctic skua in 25 km2, from which we can estimate that 60–91 birds must breed on Byers Peninsula. We also censused two colonies of gentoo penguins (3000 and 1200 pairs) and 50 pairs of chinstrap. Compared to previous estimates, gentoo penguins seem to have increased whereas chinstrap penguin have decreased. Finally, the populations of An arctic tern, southern giant petrel and kelp gull have stabilized or slightly increased.

Parasites represent ideal models for unravelling biogeographic patterns and mechanisms of diversification on islands. Both host-mediated dispersal and within-island adaptation can shape parasite island assemblages. In this study, we examined patterns of genetic diversity and structure of Ornithodoros seabird ticks within the Cape Verde Archipelago in relation to their global phylogeography. Contrary to expectations, ticks from multiple, geographically distant clades mixed within the archipelago. Trans-oceanic colonization via host movements probably explains high local tick diversity, contrasting with previous research that suggests little largescale dispersal in these birds. Although host specificity was not obvious at a global scale, host-associated genetic structure was found within Cape Verde colonies, indicating that post-colonization adaptation to specific hosts probably occurs. These results highlight the role of host metapopulation dynamics in the evolutionary ecology and epidemiology of avian parasites and pathogens.

(97) Population genetic structure and colonisation of the Western Antarctic Peninsula by the seabird tick Ixodes uriae. Marine Ecology Progress Series 459:109-120.

Recent observations on the western Antarctic Peninsula have suggested that changing climatic conditions may be increasing pressure on breeding seabirds due to higher exploitation rates by the tick Ixodes uriae. Using data from 8 microsatellite markers and ticks from 6 Pygoscelis spp. colonies, we employed a population genetics approach to specifically test the hypothesis that I. uriae is expanding south-westward along the peninsula from the Subantarctic region. Contrary to expectations, tick genetic diversity was high within all colonies, and no remaining signal of colonisation events was evident. Although significant geographic genetic structure occurred among ticks from different colonies, these ectoparasites tended to belong to 2 major genetic groups, one found principally in south-western locations (Palmer Station area) and the other in more north-eastern areas (South Shetland Islands). More central colonies showed a mixture of ticks from each genetic group, suggesting that this area represents a hybridisation zone of ticks from 2 distinct origins. A subsequent clustering analysis, including ticks from 2 Subantarctic locations, did not reveal the source population for the northern peninsula group. Overall, our data refute the hypothesis of a recent south-westward expansion of I. uriae along the peninsula and suggest that this tick has been present at more southern latitudes for an extended period of time. Further studies on the distribution and genetic characteristics of this ectoparasite around Antarctica are now required to better understand the colonisation process and predict how changing environmental conditions may affect its presence and diversity in seabird colonies.

(96) Trace me if you can: the use of intrinsic biogeochemical markers in marine top predators. Frontiers in Ecology and the Environment 10:258-266.

Ramos, R. and J.González-Solís (2012)

Impacts of human activities on marine top predators are acute. Our poor knowledge of the spatial and trophic ecology of these marine animals ultimately compromises the viability of their populations as well as our ability to use them as environmental biomonitors. Intrinsic biogeochemical markers, such as stable isotopes, fatty acids, trace elements and chemical pollutants are increasingly used to trace spatial and trophic ecology of marine top predators. Outstanding advances include the emergence of the first oceanographic isoscapes, the advent of compound-specific isotopic analyses, the improvements in diet reconstruction using Bayesian statistics and the analysis of tissues of tracked animals to ground-truth biogeochemical profiles. However, most researchers still focus only on a few tracers. Moreover, insufficient knowledge of the biogeochemical integration in tissues, fractionation and routing processes and geographic and temporal variability in baseline levels still hamper their resolution and potential as tracers of the spatial and feeding ecology of top predators.

Seabirds are facing a growing number of threats in both terrestrial and marine habitats, and many populations have experienced dramatic changes over past decades. Years of seabird research has improved our understanding of seabird populations and has informed a broader understanding of marine ecological processes. In an effort to encourage future research and guide seabird conservation science, seabird researchers from 9 nations identified the 20 highest priority research questions, which were organized into six general categories: (i) population dynamics, (ii) spatial ecology, (iii) tropho-dynamics, (iv) fisheries interactions, (v) response to global change, and (vi) management of anthropogenic impacts (focusing on invasive species, contaminants and protected areas). For each category, we provide an assessment of the current approaches, challenges and future directions. While this is not an exhaustive list of all research needed to address the myriad conservation challenges seabirds face, the results of this effort represent an important synthesis of current expert opinion across sub-disciplines within seabird ecology. As this synthesis highlights, research, in conjunction with direct management, education, and community engagement, can play an important role in facilitating the conservation and management of seabird populations and of the ocean ecosystems on which they and we depend.

Trophic ecology and movements are critical issues to understand the role of marine predators in food webs and face the challenges of their conservation. Seabird foraging ecology is increasingly studied, but small elusive species, such as those forming the ‘little shearwater’ complex, remain poorly known. We present the first study on the movements and feeding ecology of the Macaronesian shearwater Puffinus baroli combining global location sensing units, stable isotope analyses of feathers (d13C and d15N), stomach flushings and data from maximum depth gauges in a colony from the Azores archipelago (NE Atlantic). During the chick-rearing period, parents visited their nests most nights, foraged mainly south of the colony and fed at lower trophic levels than during the non-breeding period. Squid was the most diverse prey (six families and at least 10 different taxa), but species composition varied considerably between years. Two squid families, Onychoteuthidae and Argonautidae, and the fish family Phycidae accounted for 82.3% of ingested prey by number. On average, maximum dive depths per foraging trip reached up to 14.8 m, (range: 7.9-23.1 m). After the breeding period, birds dispersed offshore in all directions and up to 2500 km from the breeding colony and fed at higher trophic levels. Overall, our results indicate that the Macaronesian shearwater is a non-migratory shearwater feeding at the lowest trophic level among Macaronesian seabirds, showing both diurnal and nocturnal activity and feeding deeper in the water column than other seabirds do, principally on small schooling squid and fish. These traits contrast with those of three other Azorean procellariiformes (Cory’s shearwater Calonectris diomedea, the Madeiran storm-petrel Oceanodroma castro and Monteiro’s storm-petrel O. monteiroi), indicating ecological segregation within the Azorean seabird community.

An understanding of the non-breeding distribution and ecology of migratory
species is necessary for successful conservation. Many seabirds spend the nonbreeding
season far from land, and information on their distribution during this
time is very limited. The black-legged kittiwake, Rissa tridactyla, is a widespread
and numerous seabird in the North Atlantic and Pacific, but breeding populations
throughout the Atlantic range have declined recently. To help understand the
reasons for the declines, we tracked adults from colonies throughout the Atlantic
range over the non-breeding season using light-based geolocation.

(92) Maternal antibody persistence: a neglected life history trait with implications from albatross conservation to comparative immunology. Proceedings of the Royal Society of London B 279:2033-2041.

The evolution of different life-history strategies has been suggested as a major force constraining physiological
mechanisms such as immunity. In some long-lived oviparous species, a prolonged persistence of
maternal antibodies in offspring could thus be expected in order to protect them over their long
growth period. Here, using an intergenerational vaccination design, we show that specific maternal antibodies
can display an estimated half-life of 25 days post-hatching in the nestlings of a long-lived bird. This
temporal persistence is much longer than previously known for birds and it suggests specific properties in
the regulation of IgY immunoglobulin catabolism in such a species. We also show that maternal antibodies
in the considered procellariiform species are functional as late as 20 days of age. Using a
modelling approach, we highlight that the potential impact of such effects on population viability
could be important, notably when using vaccination for conservation. These results have broad
implications, from comparative immunology to evolutionary eco-epidemiology and conservation biology.

(91) Geographic and trophic patterns of OCs in pelagic seabirds from the NE Atlantic and the Mediterranean: a multi-species/multi-locality approach. Chemosphere 85:432-440.

Roscales, J.L., J.Muñoz-Arnanz, J.González-Solís and B.Jiménez (2011)

Trophic ecology and geographic location are crucial factors explaining OC levels in marine vertebrates, but these factors are often difficult to disentangle. To examine their relative influence, we analyzed PCBs, DDTs and stable-nitrogen isotope signatures in the blood of 10 pelagic seabird species across 7 breeding localities from the northeast Atlantic and western Mediterranean. Large scale geographic patterns emerged due to the confined character and greater historical OC inputs in the Mediterranean compared to the Atlantic basin. Spatial patterns also emerged at the regional scale within the Atlantic basin, probably associated with long-range pollutant transport. Trophic ecology, however, also was a major factor explaining OC levels. We found clear and consistent OCs differences among species regardless of the sampled locality. However, species d15N and blood OC levels were not correlated within most breeding localities. Petrel species showed significantly greater OC burdens than most shearwater species but similar trophic positions, as indicated by their similar d15N signatures. This pattern probably results from Petrel species feeding on mesopelagic fish and squid that migrate close to the sea surface at night, whereas shearwater species mainly feed on epipelagic diurnal prey. In sum, this study illustrates the lasting and unequal influence of past human activities such as PCB and DDT usage across different marine regions. In addition, our results suggest that multispecies designs are powerful tools to monitor geographic patterns of OCs and potentially useful to assess their vertical dynamics in the marine environment

(90) Seabirds and the circulation of Lyme borreliosis bacteria in the North Pacific. Vector-Borne and Zoonotic Diseases 11:1521-1527.

Seabirds act as natural reservoirs to Lyme borreliosis (LB) spirochetes and may play a significant role in the global circulation of these pathogens. While Borrelia burgdorferi s.l. (Bbsl) has been shown to occur in ticks collected from certain locations in the North Pacific, little is known about interspecific differences in exposure within the seabird communities of this region. We examined the prevalence of anti-Bbsl antibodies in 805 individuals of nine seabird species breeding across the North Pacific. Seroprevalence varied strongly among species and locations. Murres (Uria spp.) showed the highest antibody prevalence and may play a major role in facilitating Bbsl circulation at a worldwide scale. Other species showed little or no signs of exposure, despite being present in multispecific colonies with seropositive birds. Complex dynamics may be operating in this wide scale, natural host-parasite system, possibly mediated by the host immune system and host specialization of the tick vector.

Determining migratory strategies of seabirds is still a major challenge due to their relative in accessibility. Small geolocators are improving this knowledge, but not all birds can be tracked. Stable isotope ratios in feathers can help us to understand migration, but we still have insufficient baseline knowledge for linking feather signatures to movements amongst distinct water masses. To understand the migration strategies of kittiwakes Rissa tridactyla and the link between stable isotopes in feathers and the areas in which these were grown, we tracked 6 kittiwakes from Hornøya (N Norway) with light level geolocators over one year. Then we analysed the stable isotopes of carbon and nitrogen in their 1st and 7th primary feathers as well as in the 1st, 3rd, 5th, 7th and 10th primaries of 12 birds found freshly dead in the same breeding colony. After breeding, all tracked birds moved east of the Svalbard archipelago and subsequently migrated to the Labrador Sea. Thereafter, birds showed individual variation in migration strategies; three travelled to the NE Atlantic, whereas the others remained in the Labrador Sea until the end of the wintering period. Changes in stable isotope signatures from the 1st to the 10th primary feathers corresponded well to the sequence of movements during migration, and the area in which we inferred that each feather was grown. Thus, by combining information on moult patterns and tracking data, we demonstrate that stable isotope analysis of feathers can be used to trace migratory movements of seabirds.

PAHs were analyzed in the liver of 5 species of pelagic seabirds (Procellariiformes) from the northeast
Atlantic and the Mediterranean. The main objective was to assess the trophic and geographic trends of
PAHs in seabirds to evaluate their suitability as bioindicators of chronic marine pollution by these
compounds. Although higher levels of PAHs have been described in the Mediterranean compared to
other oceanic regions, we did not find significant spatial patterns and observed only minor effects of the
geographic origin on seabird PAHs. However, we found significant higher PAH levels in petrel compared
to shearwater species, which could be related to differences in their exploitation of mesopelagic and
epipelagic resources, respectively, and the vertical dynamic of PAHs in the water column. Overall,
although this study enhances the need of multi-species approaches to show a more comprehensive
evaluation of marine pollution, seabirds emerged as poor indicators of pelagic chronic PAH levels.

(87) Feather corticosterone of a nestling seabird reveals consequences of sex-specific parental investment. Proceedings of the Royal Society of London B 279:177-184.

Offspring of long-lived species should face costs of parental trade-offs that vary with overall energetic
demands encountered by parents during breeding. If sex differences exist in how parents make the
trade-off, sex-specific differences may exist in the contribution of each parent to those costs. Adaptations
of offspring facing such costs are not well understood, but the hormone corticosterone probably plays
a role. We manipulated breeding effort in Cory’s shearwaters (Calonectris diomedea) to increase costs to
offspring and used an integrated measure of corticosterone from chick feathers to investigate how experimental
variation in parental investment influences offspring physiology. Average foraging trip duration
and foraging efficiency (FE) of breeding pairs were not related to chick corticosterone, but sex biases
in FE were. Adult male investment was more strongly related to chick corticosterone than was female
investment. Importantly, we show for the first time suppression of adrenocortical activity in nestling
Procellariiform seabirds, and explain how our results indicate an adaptive mechanism invoked by
chicks facing increased costs of parental trade-offs.

FEATURE ARTICLE

Roscales, J.L., E.Gómez-Díaz, V.Neves and J.González-Solís (2011)

Feeding ecology and geographic location
are 2 major factors influencing animal stable isotope signatures,
but their relative contributions are poorly understood,
which limits the usefulness of stable isotope
analysis in the study of animal ecology. To improve our
knowledge of the main sources of isotopic variability at
sea, we determined ?15N and ?13C signatures in the first
primary feather of adult birds from 11 Procellariiform
species (n = 609) across 16 northeast Atlantic localities,
from Cape Verde (20° N) to Iceland (60° N). Post-breeding
areas (where the studied feather is thought to be
grown) were determined using light-level geolocation
for 6 of the 11 species. Isotopic variability was geographically
unstructured within the mid-northeast Atlantic
(Macaronesian archipelagos), but trophically structured
according to species and regardless of the breeding
location, presumably as a result of trophic segregation
among species. Indeed, the interspecific isotopic overlap
resulting from combining ?15N and ?13C signatures of
seabirds was low, which suggests that most species exploited
exclusive trophic resources consistently across
their geographic range. Species breeding in north temperate
regions (Iceland, Scotland and Northern Ireland)
showed enriched ?15N compared to the same or similar
species breeding in tropical and subtropical regions,
suggesting some differences in baseline levels between
these regions. The present study illustrates a noticeable
trophic segregation of northeast Atlantic Procellariiformes.
Our results show that the isotopic approach has
limited applicability for the study of animal movements
in the northeast Atlantic at a regional scale, but is potentially
useful for the study of long-distance migrations
between large marine systems.

Carotenoids, as pigments with antioxidant and
immunoregulatory properties, play a crucial role in
developing chicks. Carotenoids must be acquired through
diet and are relatively scarce, suggesting that their
availability is a limiting factor leading to a trade-off
between colour displays and physiological functions.
However, potential differences in this trade-off between
male and female chicks have been little studied. We
manipulated carotenoid availability in 9 days old common
tern Sterna hirundo chicks by supplementing their fish
diet with four carotenoids during 9 days. Our aim was to
examine sex-specific responses to the experimental
increase of dietary carotenoids on plasma circulation,
physiological and condition variables and successful
fledging. Furthermore, to explore the functional and
evolutionary basis of the trade-off, we studied the relationships
among carotenoid concentration, mediated
immune response and foot colouration. After treatment,
control chicks showed decreasing plasma levels for most
carotenoid types, whereas supplemented chicks had strong
increases. Colour luminosity and saturation increased in
both treatment groups, while hue only changed significantly
towards redder feet in supplemented females.

Identifying markers that are indicative of individual state, related to fitness, and which could be used to study life-history tradeoffs
in wild populations is extremely difficult. Recently, it has been suggested that telomeres, the ends of eukaryote chromosomes,
might be useful in this context. However, little is known of the link between telomere length and fitness in natural populations
and whether it is a useful indicator of biological state. We measured average telomere length in red blood cell samples taken from
a wide age range of individuals of a very long-lived and highly sexually dimorphic seabird, the southern giant petrel (Macronectes
giganteus). We examined the relationship with age, sex, and subsequent survival over an 8-year period. Telomere length was
longer in chicks than adults. Within the adult group, which ranged in age from 12 to 40 years, telomere length was not related to
age. For the first time in birds, there was some evidence of a sex difference. Male giant petrels, which are substantially larger than
females, had significantly shorter telomere lengths than females. This difference was evident from an early stage in life and is
likely to relate to differences in growth trajectories. Those adults that died during the 8-year time window following the telomere
length measurement had significantly shorter telomere lengths than those that survived this period, irrespective of age or sex,
neither of which were significant predictors of survival. These results show that relatively short telomere length is related to future
life expectancy at any adult age, demonstrating its usefulness as a state variable. Key words: giant petrel, life span, sex differences,
survival, telomere dynamics.

The loss of species during the Holocene was, dramatically more important on islands than on continents. Seabirds from islands are very vulnerable to human-induced alterations such as habitat destruction, hunting and exotic predators. For example, in the genus Puffinus (family Procellariidae) the extinction of at least five species has been recorded during the Holocene, two of them coming from the Canary Islands.

(82) Physiological ecology of breeders and sabbatical breeders in a pelagic seabird. Journal of Experimental Marine Biology and Ecology 389:13-17.

Giudici, A, J.Navarro, C.Juste and J.González-Solís (2010)

In long-lived seabirds, intermittent nonbreeding years in an adult life, the so called ‘sabbatical years’, are
common. However, proximal causes that make animals decide whether to breed or not to breed are poorly
known. In this study we assessed different components of the physiological state of a long-lived pelagic
seabird, the Cory's shearwaters Calonectris diomedea, during the pre-laying period to understand which
components are influencing breeding decisions. We analyzed plasma biochemistry, haematocrit, leukocyte
counts and stable isotope (?15N and ?13C) values in blood and claws. We also recorded body mass,
ectoparasite loads and fault bars in primary and tail feathers. All parameters were compared between
breeders and sabbaticals and between males and females. Among all biochemical variables analyzed we
found triglyceride levels in plasma to be significantly greater in breeders than in sabbaticals and in females
than males. H/L ratio was lower in breeders than in sabbaticals and greater in males than females. These
results suggest the ability to store fat and the immunological condition are important components
compromising breeding decisions. Our study also suggests that H/L ratio can be used as a good predictor of
the breeding decisions.

Ecological studies on food webs rarely include parasites, partly due to the complexity and dimensionality of host-parasite interaction networks. Multiple co-occurring parasites can show different feeding strategies and thus lead to complex and cryptic trophic relationships, which are often difficult to disentangle by traditional methods. We analyzed stable isotope ratios of C (13C/12C, ?13C) and N (15N/14N, ?15N) of host and ectoparasite tissues to investigate trophic structure in 4 co-occurring ectoparasites: three lice and one flea species, on two closely related and spatially segregated seabird hosts (Calonectris shearwaters). ?13C isotopic signatures confirmed feathers as the main food resource for the three lice species and blood for the flea species. All ectoparasite species showed a significant enrichment in ?15N relatively to the host tissue consumed (discrimination factors ranged from 2 to 5‰ depending on the species). Isotopic differences were consistent across multiple host-ectoparasite locations, despite of some geographic variability in baseline isotopic levels. Our findings illustrate the influence of both ectoparasite and host trophic ecology in the isotopic structuring of the Calonectris ectoparasite community. This study highlights the potential of stable isotope analyses in disentangling the nature and complexity of trophic relationships in symbiotic systems.

Although seabirds have been proposed as useful biomonitors
for organochlorine contaminants (OCs) in marine environments,
their suitability is still unclear. To understand the geographic
variability and the influence of seabird trophic ecology in OC
levels, we analyzed PCBs, DDTs, ?13C, and ?15N in the blood of
adult Calonectris shearwaters throughout a vast geographic
range within the northeast Atlantic Ocean (from Cape Verde to
Azores) and the Mediterranean Sea (from the Alboran Sea
to Crete). OC concentrations were greater in birds from the
Mediterranean than in those from the Atlantic colonies, showing
higher and lower chlorinated PCB profiles, respectively. This
large-scale patternmayreflect theinfluenceof historicalEuropean
runoffs in the Mediterranean basin and diffused sources for
OCs in remote Atlantic islands. Spatial patterns also emerged
withintheAtlanticbasin,probablyassociatedwithpollutantlongrange
transport and recent inputs of DDT in the food webs of
shearwaters from Cape Verde and the Canary islands.
Moreover, a positive association of OC concentrations with
?15N within each locality points out diet specialization as a major
factor explaining differences in OCs at the intraspecific level.
Overall, this study highlights wide range breeding seabirds, such
as Calonectris shearwaters, as suitable organisms for
biomonitoring large geographic trends of organochlorine
contamination in the marine environment.

(79) Effects of extra mass on the pelagic behaviour of a seabird. Auk 127: 100-107

Passos, C., A.Giudici, J.Navarro., J.González-Solís (2010)

Satellite transmitters and geographic-positioning-system devices often add substantial mass to birds to which they
are attached. Studies on the effects of such instruments have focused on indirect measures, whereas the direct influence of extra mass
on pelagic behavior is poorly known. We used 2.5-g geolocators to investigate the effect of extra mass on the pelagic behavior of Cory’s
Shearwaters (Calonectris diomedea) by comparing the traits of a single foraging trip among a group carrying 30-g weights, a group
carrying 60-g weights, and a control group. The weights were attached to the birds’ backs using typical techniques for attaching satellite
transmitters to seabirds. The extra mass increased the duration of the birds’ trips and decreased their foraging efficiency and mass
gained at sea. These indirect effects may be related to foraging traits: weighted birds showed a greater search effort than control birds,
traveled greater distances, covered a greater foraging area, and increased the maximum foraging range. Furthermore, the time spent
on the sea surface at night was greater for weighted than for control groups, which showed that the extra mass also affected activity
patterns. Our results underline the need to quantify the effects of monitoring equipment commonly used to study the pelagic behavior
of seabirds. We suggest that geolocators can be used to obtain control data on foraging-trip movements and activity patterns.

To test the potential effects of winds on the migratory detours of shearwaters, transequatorial
migrations of 3 shearwaters, the Manx Puffinus puffinus, the Cory’s Calonectris diomedea, and
the Cape Verde C. edwardsii shearwaters were tracked using geolocators. Concurrent data on the
direction and strength of winds were obtained from the NASA SeaWinds scatterometer to calculate
daily impedance models reflecting the resistance of sea surface winds to the shearwater movements.
From these models we estimated relative wind-mediated costs for the observed synthesis pathway
obtained from tracked birds, for the shortest distance pathway and for other simulated alternative
pathways for every day of the migration period. We also estimated daily trajectories of the minimum
cost pathway and compared distance and relative costs of all pathways. Shearwaters followed 26 to
52% longer pathways than the shortest distance path. In general, estimated wind-mediated costs of
both observed synthesis and simulated alternative pathways were strongly dependent on the date of
departure. Costs of observed synthesis pathways were about 15% greater than the synthesis pathway
with the minimum cost, but, in the Cory’s and the Cape Verde shearwaters, these pathways were
on average 15 to 20% shorter in distance, suggesting the extra costs of the observed pathways are
compensated by saving about 2 travelling days. In Manx shearwaters, however, the distance of the
observed synthesis pathway was 25% longer than that of the lowest cost synthesis pathway, probably
because birds avoided shorter but potentially more turbulent pathways. Our results suggest that
winds are a major determinant of the migratory routes of seabirds.

The spatial ecology of seabirds has greater precedence today than ever before because
of impacts on the marine environment from human exploitation, pollution, and climate change. Specific
life history traits make seabirds particularly sensitive to these impacts, currently driving many
species to unsustainable population declines. To evaluate the risk posed by human activities, we
need integrative studies on seabird abundance, distributions, and movements in relation to the biophysical
marine environment. Also interpreting the limits of these relationships is fundamental to
understanding historical constraints and behavioural adaptations of seabirds that are shaped by evolutionary
processes. In this Theme Section, we assembled 4 review papers and 10 case studies that
highlight some of the latest techniques to study seabird spatial ecology. This includes the application
of tracking tags, ship-based surveys, and remotely sensed environmental data, to characterize
seabird movement patterns, fine scale behaviour, and overall distribution combined with measures of
the oceanic habitats to enhance our understanding of the functional role that seabirds play. Overall,
this knowledge is crucial for understanding and predicting the impacts that fisheries, climate change
and pollution are exerting on marine ecosystems and will provide opportunities for developing
marine protected areas, conservation action plans and species management.

The Patagonian Sea is the oceanic ecosystem of the South-Western Atlantic. Exposed to the ecological effects of the fronts created by the Brazil and Malvinas currents, it is a unique, highly productive ocean which is plentiful in species of high aesthetic, ecological and economic value, but it is also vulnerable. Albatrosses, petrels, penguins, sea lions and elephant seals are top predators and charismatic species of the Patagonian Sea. They require considerable space and resources and are vulnerable to many human activities. This makes them good indicators of the status of conservation of the ecosystem; ensuring their protection will also be beneficial for many other species. However, current conservation measures, which comprise relatively few impact mitigation actions and limited protection of coastal breeding areas, are insufficient. There is an urgent need to identify and protect the open sea environments where turtles, seabirds and marine mammals forage. This Atlas of the Patagonian Sea. Species and Spaces is a summary of information on the use made of the ecosystem of the Patagonian Sea by 16 species of top predators: Wandering Albatross, Black-browed Albatross, Grey-headed Albatross, Northern Royal Albatross, Light-mantled Albatross, Southern Giant Petrel, Northern Giant Petrel, White-chinned Petrel, Magellanic Penguin, Southern Rockhopper Penguin, King Penguin, Gentoo Penguin, South American Sea Lion, South American Fur Seal, Antarctic Fur Seal and Southern Elephant Seal. Identification of the principal foraging areas for these species was made possible by the use of tracking methods that allow the movements of individuals in the ocean to be closely observed and accurately described. The key coastal areas involved are the waters adjacent to the Malvinas Islands, the Staten Island and the Diego Ramírez Islands, and the Valdés Peninsula as far as the northern end of the Gulf of San Jorge. These areas have been recognised as being significant for the biological diversity of the Patagonian Sea. Far from the coast, and therefore out of sight, pelagic environments are less well known and thus tend to be ignored. The data included in this publication suggest as important areas for top predators the oceanographic front associated with the slope area of the Patagonian Shelf, the ocean environment adjacent to the Malvinas Islands, the shelf-slope area at the latitude of the Gulf of San Jorge and areas influenced by the outflow of the Río de la Plata. Also important are the waters to the east of the Burdwood Bank and those of the polar front in the far south-east of the Patagonian Sea. A coastal-pelagic corridor between the Valdés Peninsula and the slope appears to be particularly important for migratory movements from the coast to the deep sea. Among the spatial tools for protecting the open sea are the creation of marine areas under special management. Any definition on the use of resources in these areas should embody the concept of integrated ecosystem management in the conservation of the diversity of species and ecological functions. The ecological requirements of top predators are rarely taken into account when defining management actions, except when evidence has been produced of potentially irreversible impact. This Atlas helps to identify those marine environments for which considerations of integrated management are urgently needed. The Patagonian Sea is not pristine. The unsustainable exploitation of its resources means that numerous human activities pose threats to biodiversity. These include overfishing, fishing discards, the bycatch of seabirds and marine mammals, the introduction of non-native species, the degradation of coastal and marine environments, and the contamination associated with the extraction of oil, or caused by urban and industrial waste. The current situation of the seas of the world, under even greater threat as a result of climate change, cannot tolerate any further inaction. The biological diversity and functionality of the world’s ecosystems are of extraordinary importance for the future of humankind. However, the “biodiversity crisis” that could bring about the collapse and extinction of certain animal populations clearly exists, and appropriate solutions have yet to be found. The detailed analysis contained in this Atlas of the Patagonian Sea provides useful basic scientific information essential for implementing the vision of integrated ecosystem management. In particular it suggests that there is a need to create a network of marine areas, under special considerations of biodiversity management, which will incorporate those open sea environments which are linked to key coastal areas.

Migratory routes and wintering areas of Cory’s shearwaters
Calonectris diomedea from es Pantaleu (Mallorca). Here we show the migratory
trips through the Atlantic Ocean of 8 Cory’s shearwaters breeding at es Pantaleu
islet (P.N. de sa Dragonera, Majorca) during the winter of 2002-2003. The
majority of the birds preferred the coasts of the Sahara and of Mauritania and to
a lesser extent those of Namibia, corresponding to two zones of oceanic
upwelling of cold and very productive waters (Canary and Benguela currents
respectively). One of the birds behaved more in keeping with those from the
Atlantic colonies, wintering in the confluence of the Brazilian and Malvinas currents
off the coasts of southern Brazil and Uruguay, a straight-line distance of
some 9000 km from es Pantaleu. Another spent the winter in the equatorial
waters of the gulf of Guinea. Most of the birds made a loop-migration return
through the northern sub-equatorial Atlantic, avoiding the calm areas. One of
them reached the coasts of the Small Antilles, in the Caribbean.
Key words: Cory’s shearwaters, Calonectris diomedea, migratory routes, wintering
areas, es Pantaleu (Mallorca).
Paraules clau: virot gros, Calonectris diomedea, rutes migratòries, àrees
d’hivernada, es Pantaleu (Mallorca).

On 21 March 2007, while catching Fea’s Petrels
Pterodroma feae on Fogo, Cape Verde Islands, Jacob Gonzalez-Solis noticed an odd individual amongst 17 birds trapped for ringing which showed an overall grey cast to the entire underparts.
In spring 2008 and 2009, respectively, a
further 18 and 19 were trapped but none showed any anomalous coloration.

Trophic segregation has been proposed as a major
mechanism explaining the coexistence of closely
related animal taxa. However, how such segregation
varies throughout the annual cycle is
poorly understood. Here, we examined the
feeding ecology of the two subspecies of Cory’s
shearwater, Calonectris diomedea diomedea
and Calonectris diomedea borealis, breeding in
sympatry in a Mediterranean colony. To study
trophic segregation at different stages, we combined
the analysis of isotope values (d15N, d13C) in
blood obtained during incubation and in feathers
moulted during chick-rearing and wintering
periods with satellite-tracking data during the
chick-rearing period. Satellite-tracking and stable
isotope data of the first primary feather revealed
that C. d. borealis foraged mainly in the Atlantic
whereas C. d. diomedea foraged exclusively in the
Mediterranean. This spatial segregation could
reflect the foraging behaviour of the C. d. borealis
individuals before they arrived at the Mediterranean
colony. Alternatively, greater wing loading of
C. d. borealis individuals may confer the ability to
fly across the strong winds occurring at the at the
Gibraltar strait. Isotope values of the eighth secondary
feather also support segregation in wintering
areas between the two forms: C. d. diomedea
wintered mainly in association with the Canary
current, whereas C. d. borealis wintered in the
South African coast. Overall, our results show that
spatial segregation in foraging areas can display
substantial variation throughout the annual cycle
and is probably a major mechanism facilitating
coexistence between closely related taxa.

(71) Population structure in a highly pelagic seabird, the Cory’s shearwater (Calonectris diomedea): an examination of genetics, morphology and ecology. Marine Ecology Progress Series 382: 197-209.

Gómez-Díaz, E., J.González-Solís and M.A.Peinado (2009)

Increasing evidence suggests oceanic traits may play a key role in the genetic structuring
of marine organisms. Whereas genetic breaks in the open ocean are well known in fishes and
marine invertebrates, the importance of marine habitat characteristics in seabirds remains less certain.
We investigated the role of oceanic transitions versus population genetic processes in driving
population differentiation in a highly vagile seabird, the Cory’s shearwater, combining molecular,
morphological and ecological data from 27 breeding colonies distributed across the Mediterranean
(Calonectris diomedea diomedea) and the Atlantic (C. d. borealis). Genetic and biometric analyses
showed a clear differentiation between Atlantic and Mediterranean Cory’s shearwaters. Ringingrecovery
data indicated high site fidelity of the species, but we found some cases of dispersal among
neighbouring breeding sites (<300 km) and a few long distance movements (>1000 km) within and
between each basin. In agreement with this, comparison of phenotypic and genetic data revealed
both current and historical dispersal events. Within each region, we did not detect any genetic substructure
among archipelagos in the Atlantic, but we found a slight genetic differentiation between
western and eastern breeding colonies in the Mediterranean. Accordingly, gene flow estimates suggested
substantial dispersal among colonies within basins. Overall, genetic structure of the Cory’s
shearwater matches main oceanographic breaks (Almería-Oran Oceanic Front and Siculo-Tunisian
Strait), but spatial analyses suggest that patterns of genetic differentiation are better explained by
geographic rather than oceanographic distances. In line with previous studies, genetic, phenotypic
and ecological evidence supported the separation of Atlantic and Mediterranean forms, suggesting
the 2 taxa should be regarded as different species.

The value of stable isotope analysis in tracking
animal migrations in marine environments is poorly
understood, mainly due to insufficient knowledge of isotopic
integration into animal tissues within distinct water
masses. We investigated isotopic and moult patterns in
Cory’s shearwaters to assess the integration of different
stable isotopes into feathers in relation to the birds’ transoceanic
movements. Specimens of Mediterranean Cory’s
shearwater Calonectris diomedea diomedea caught accidentally
by Catalan longliners were collected and the
signatures of stable isotopes of C (d13C), N (d15N) and S
(d34S) were analysed in 11 wing and two tail feathers from
20 birds, and in some breast feathers. Based on isotopic
signatures and moult patterns, the feathers segregated into
two groups (breeding and wintering), corresponding to
those grown in the Mediterranean or Atlantic regions,
respectively. In addition, feathers grown during winter, i.e.
moulted in Atlantic waters, were grouped into two isotopically
distinct profiles, presumably corresponding to the
two main wintering areas previously identified for Mediterranean
Cory’s shearwater in tracking studies. N
signatures mainly indicated the Mediterranean-to-Atlantic
migration, whereas C and S signatures differed according to
the Atlantic wintering area. Our results indicate that isotopic
signatures from distant oceanic regions can integrate the
feathers of a given bird and can indicate the region in which
each feather was grown. This study thus underscores how
stable isotope analysis can link marine animals to specific
breeding and wintering areas, and thereby shed new light on
studies involving assignment, migratory connectivity and
carry-over effects in the marine environment.

Global wind patterns influence dispersal and migration processes of aerial organisms, propagules and particles, which ultimately could determine the dynamics of colonizations, invasions or spread of pathogens. However, studying how wind?mediated movements actually happen has been hampered so far by the lack of high resolution global wind data as well as the impossibility to track aerial movements. Using concurrent data on winds and actual pathways of a tracked seabird, here we show that oceanic winds define spatiotemporal pathways and barriers for large?scale aerial movements. We obtained wind data from NASA SeaWinds scatterometer to calculate wind cost (impedance) models reflecting the resistance to the aerial movement near the ocean surface. We also tracked the movements of a model organism, the Cory’s shearwater (Calonectris diomedea), a pelagic bird known to perform long distance migrations. Cost models revealed that distant areas can be connected through “wind highways” that do not match the shortest great circle routes. Bird routes closely followed the low?cost “wind?highways” linking breeding and wintering areas. In addition, we found that a potential barrier, the near surface westerlies in the Atlantic sector of the Intertropical Convergence Zone (ITCZ), temporally hindered meridional trans?equatorial movements. Once the westerlies vanished, birds crossed the ITCZ to their winter quarters. This study provides a novel approach to investigate wind?mediated movements in oceanic environments and shows that large?scale migration and dispersal processes over the oceans can be largely driven by spatiotemporal wind patterns.

Despite the advent of devices to track seabird movements, the extent to which productive areas and oceanic winds influence foraging strategies is still not fully understood. We investigated the main environmental determinants of foraging strategies in Cory’s shearwaters Calonectris diomedea. We combined satellite-tracking information from 14 birds breeding on Canary Island with concurrent data on chlorophyll concentrations and oceanic winds. Additionally, we took a blood sample at the end of each foraging trip and analysed stable isotope signatures to examine the use of trophic resources. All birds showed a commuting type of trip, concentrating foraging activity exclusively on the African continental shelf. Foraging locations showed a strong association with chlorophyll concentrations, suggesting birds select foraging areas according to prey availability. In contrast with other breeding colonies where Cory’s shearwaters use a dual-foraging strategy, birds showed a unimodal strategy and did not show differences in C and N isotope signatures in plasma, confirming that close proximity to highly productive areas strongly influence foraging strategies. In addition, birds tracked during two consecutive trips foraged on the same area, suggesting high resource availability promotes fidelity to feeding grounds also at coarse-scales. Commuting trips followed a consistent clockwise movement, heading southwest when birds foraged along the continental shelf. Trade winds consistently blow from northeast over the study period, suggesting that birds used tail winds to reduce their flying costs. In summary, our results corroborate that oceanographic conditions in the vicinity of the breeding colony have a strong effect on foraging strategies of pelagic seabirds.
KEY WORDS: chlorophyll a, foraging movements, oceanic productivity, oceanic wind, satellite-tracking, stable isotopes.

Seabird moult is poorly understood because most species undergo moult at sea during the non-breeding season. We scored moult of wings, tail and body feathers on 102 Mediterranean Cory’s Shearwaters accidentally caught by longliners throughout the year. Primary renewal was found to be simple and descendant from the most proximal (P1) to the most distal (P10) feather. Secondaries showed a more complex moulting pattern, with three different asynchronous foci: the first starting on the innermost secondaries (S21), the second on the middle (S5) and the latest on the outermost secondaries (S1). Rectrix moult started at a later stage and was simple and descendant from the most proximal feather (R1) expanding distally. Although a few body feathers can be moulted from pre-laying to hatching, moult of ventral and dorsal feathers clearly intensified during chick rearing. Different moulting sequences and uncoupled phenology between primary and secondary renewal suggest that flight efficiency is a strong constraint factor in the evolution of moulting strategies. Moreover, moult of Cory’s Shearwaters was synchronous between wings and largely asynchronous between tail halves, with no more than one rectrix moulted at once. This result is probably related to the differential sensitivity of wings and the tail on flight performance, ultimately derived from different aerodynamic functions. Finally, Cory’s Shearwater females renewed feathers earlier and faster than males, which may be related to the lower chick attendance of females.

(66) The influence of breeding colony and sex on mercury, selenium and lead levels and carbon and nitrogen stable isotope signatures in summer and winter feathers of Calonectris shearwaters. Oecologia 159:345-354.

Contamination in marine foodwebs is nowadays of great environmental concern owing to the
increasing pollution levels in marine ecosystems from different anthropogenic sources. Seabirds can be used as indicators of regional contaminant patterns across large temporal and spatial scales. We analysed mercury, selenium and lead levels as well as stable isotope ratios of carbon and nitrogen in breeding- and winter-season feathers on males and females of two related shearwater species, providing information on spatiotemporal patterns of contaminants as well as the influence of the trophic ecology of these seabirds on contaminant levels. During the breeding season, selenium and lead concentrations were highest at Cape Verde archipelago, showing no differences among the other colonies or between the sexes. However, mercury levels varied among colonies, being highest in the Mediterranean, probably resulting from the larger emissions and fallout of this pollutant in Europe. Feathers grown during breeding also showed sexual differences in mercury concentrations and D13C. Differences in mercury concentration between sexes are mainly due to egg-laying decontamination in females. In contrast, differences in mercury among colonies are probably related to differences in trophic ecology, as indicated by D13C and D15N measurements. Contaminant concentrations in winter-grown feathers did not show any relationship with stable isotope values but were affected by contaminant loads associated with the breeding season. These findings suggest that the interpretation of contaminant levels of migratory species from feathers moulted out of the breeding season should be made with caution because those values could reflect exposures to contaminants acquired during the breeding season. We conclude that other factors than feeding ecology may play an important role in the interpretation of contaminant levels and their annual dynamics at several spatial scales. Consideration of the relevant temporal context provided by isotopic signatures and contaminant concentrations is important in deciphering contaminant information based on various tissues.

The bill is a sexually dimorphic structure in many bird species implicated in numerous functions. Sexual differences may arise from sexual selection or ecological divergence. Here, we tested the relationship between sexual dimorphism and trophic niche segregation in Cory’s shearwaters. For the first time in avian morphological studies, we combined linear measurements and geometric morphometric methods to examine sexual differences in bill size and shape. We also investigated feeding ecology by tracking foraging movements during the breeding period and by analysing stable isotope signatures in blood during the breeding and in feathers grown during the non breeding period. Bill traits were all sexually dimorphic, both in absolute and relative terms, and scaled hypermetrically with body mass in several characters in males. However, males and females did not differ in their feeding areas or isotopic signatures and no significant correlation was observed between these traits and bill dimorphism. Therefore, we discard the foraging-niche divergence hypothesis, and suggest that sexual dimorphism in bill size in this species is more likely driven by sexual selection related to antagonistic interactions.

Parasite communities can be structured at different spatial scales depending on the level of organization of the hosts; hence, examining this structure should be a multiscale process. We investigated ectoparasite community structure composed by three lice (Halipeurus abnormis, Austromenopon echinatum and Saemundssonia peusi) and one flea species (Xenopsylla gratiosa) at the infra-, component and regional community levels in three closely related seabird hosts, the Mediterranean Cory’s shearwater (Calonectris d.diomedea), the Atlantic Cory’s shearwater (C.d.borealis) and the Cape Verde shearwater (C.edwardsii). We examined temporal and spatial structuring of infracommunities, the influence of host aggregation and body condition on the component community, and the effect of genetic and geographic connectivity among host populations on the regional community. Ectoparasite infracommunities showed a substantial species overlap in temporal patterns of abundance but species were spatially segregated within the host body. Within component communities, abundance of all ectoparasite species showed an aggregated distribution. However, aggregation patterns and its relationship with the spatial distribution of hosts within the breeding colony differed among ectoparasite species, mainly reflecting ecological differences between fleas and lice. At regional scale, similarity in ectoparasite communities correlated with geographic distances among host colonies, but not with genetic distances. This result suggests differences in climate and habitat characteristics among host localities as a major determinat of regional communities, rather than host connectivity. Our results highlight the importance of the geographic distribution of host breeding colonies and the spatial segregation within the host body as key factors in determining ectoparasite community structure in Calonectris shearwaters.

(63) Ecophysiological responses to an experimental increase of wing loading in a pelagic seabird. Journal of Experimental Marine Biology and Ecology 358:14-19.

Navarro, J., J.González-Solís, G.Viscor & O.Chastel (2008)

The knowledge of ecophysiological responses in relation to foraging effort is crucial to understanding feeding strategies, survival and reproductive trade-offs, as well as to obtain reliable indicators of an excessive workload. We present an integrative approach that examines a suite of ecophysiological parameters in relation to increased workload. We experimentally increased wing loading of 10 Cory's shearwaters Calonectris diomedea, a medium-sized pelagic seabird, by adding 45 g extra weight and compared their ecophysiological responses with 10 control birds. Among all the parameters analysed, the only significant response to overloading was a longer foraging trip, a lower rate of mass gain whilst at sea, and an increase in plasma levels of creatine kinase and lactate dehydrogenase activity indicating muscular damage. The analyses on these muscular enzymes open new opportunities to measure the impact of instruments on birds and to understand physiological responses in relation to foraging activity.

We examined whether the two giant petrel species segregate by gender and species in relation to the stage of the annual cycle. We tracked the individual foraging behavior of 14 male and 11 female northern giant petrels (Macronectes halli) and 13 male and 15 female southern giant petrels (M.giganteus) breeding at South Georgia over one year by using geolocators (Global Location System loggers). Males of both species showed a flexible foraging strategy, switching from coastal to pelagic habits, probably governed by spatiotemporal changes in carrion availability. In contrast, marine areas exploited by females were more consistent over the year and similar between the two species, with most foraging locations concentrated over the same pelagic waters. This study provides support for the differences in foraging between sexes as the main mechanism to reduce intraspecific competition. Although the two species are morphologically similar and can easily access each other’s foraging habitat, they differ in the foraging areas exploited. Thus, interspecific competition seems mainly relaxed by spatial segregation, particularly between males in winter, probably mediated by different competitive abilities and physical tolerances to temperature and winds. Foraging southern giant petrels from South Georgia were not restricted to the areas within the line of equidistance to other colonies, but their foraging range overlapped with feeding grounds of conspecifics breeding in the Falkland Islands and the Antarctic Continent. Taken together, these findings suggest that foraging selection on marine habitat heterogeneity reduces interspecific competition, whereas carrion availability reduces intersexual competition, in giant petrels.

(61) Sympatric speciation by allochrony in the Madeiran storm petrel. Proceedings of the National Academy of Sciences, USA, 104:18589-18594.

The importance of sympatric speciation (the evolution of reproductive isolation between
codistributed populations) in generating biodiversity is highly controversial. Whereas
potential examples of sympatric speciation exist for plants, insects and fishes, most
theoretical models suggest that it requires conditions that are probably not common in
nature, and only two possible cases have been described for tetrapods. One mechanism by
which it could occur is through allochronic isolation - separation of populations by breeding
time. Oceanodroma castro (the Madeiran or band-rumped storm-petrel) is a small seabird
that nests on tropical and subtropical islands throughout the Atlantic and Pacific Oceans.
In at least five archipelagos, different individuals breed on the same islands in different
seasons. We compared variation in five microsatellite loci and the mitochondrial control
region among 562 O. castro from throughout the species’ range. We found that sympatric
seasonal populations differ genetically within all five archipelagos, and have ceased to
exchange genes in two. Population and gene trees all indicate that seasonal populations
within four of the archipelagos are more closely related to each other than to populations
from the same season from other archipelagos; divergence of the fifth sympatric pair is too
ancient for reliable inference. Thus, seasonal populations appear to have arisen
sympatrically at least four times. This is the first evidence for sympatric speciation by
allochrony in a tetrapod, and adds to growing indications that population differentiation
and speciation can occur without geographic barriers to gene flow.

We compared patterns of mitochondrial DNA differentiation in three host-specific lice (Halipeurus abnormis, Austromenopon echinatum and Saemundssonia peusi) and one generalist flea (Xenopsylla gratiosa), parasitizing 22 colonies of Calonectris. The three closely related hosts: the Atlantic and the Mediterranean Cory’s (Calonectris d. diomedea and C. d. borealis) and the Cape Verde (C. edwardsii) shearwaters show distinct phylogeographic structure. The host-specific lice appeared undifferentiated among the three Calonectris taxa, whereas the more generalist flea displayed significant levels of population differentiation. Neither genetic distances among host populations, nor their spatial distribution explained the patterns of genetic variability observed in the ectoparasites. The lack of differentiation among lice is unexpected, given that seabird lice show high levels of cospeciation with their hosts, and have an elevated rate of mitochondrial DNA evolution. We discuss the implications of these findings.

In birds, parents adjust their feeding behaviour to the breeding duties, which ultimately may lead to seasonal adjustments in nutritional physiology and hematology over the breeding season. Although avian physiology has been widely investigated in captivity, few studies integrate individual changes in feeding and physiological ecology throughout the breeding season in wild birds. To study relationships between feeding ecology and nutritional ecophysiology in Cory’s shearwaters (Calonectris diomedea), we weighed and took blood samples at pre-laying, egg-laying, mid incubation, hatching and chick rearing of 28 males and 19 females. In addition, we instrumented 6 birds with geolocators to track their foraging movements throughout the reproductive period. Thus, we examined individual changes in (a) nutritional condition (biochemistry metabolites); (b) oxygen carrying capacity (hematology) and; (c) feeding areas and foraging effort (stable isotopes and foraging movements). Geolocators revealed a latitudinal shift in main feeding areas towards more southern and more neritic waters throughout the breeding season, which is consistent with the steady increase in ?13C signatures found in blood. Geolocators also showed a decrease in foraging effort from egg-laying to hatching, reflecting the activity decrease associated to the incubation duties. Plasma metabolites, body mass and oxygen carrying capacity were associated to temporal changes in nutritional state and foraging effort, in relation to the recovery after migration, egg formation, fasting shifts during incubation and chick provisioning. This study shows that combining physiological and ecological approaches can help understanding the influence of breeding duties on feeding ecology and nutritional physiology in wild birds.

It has been suggested that sexual size dimorphism (SSD) may influence sex ratios at different life stages. Higher energy requirements during growth associated with larger body size could lead to a greater mortality of the larger sex and ultimately to an overproduction of the smaller sex. To explore the associations between SSD and hatching and fledging sex ratio we performed a species-level and a phylogenetically controlled analysis, based on 83 bird species. Overall, there was a significant inverse relationship between the degree of SSD and the proportion of males at hatching and fledging. Sex-specific mortality related to SSD showed a weak but persistent negative tendency, suggesting a mortality bias towards the larger sex. These results suggest that changes in relation to SSD may take place mainly at the conception stage, but could be adjusted during growth. However, conclusions should be cautiously treated since these relationships weaken when additional variables are considered.

Longline fisheries, oil spills and offshore wind mills are some of the major threats increasing seabird mortality at sea, but the impact of these threats on specific populations have been difficult to determine so far. We tested the use of molecular markers, morphometric measures, and stable isotope (?15N and ?13C) and trace element concentrations in the first primary feather (grown at the end of the breeding period) to assign the geographic origin of Calonectris shearwaters. Overall, we sampled birds from three taxa: 13 Mediterranean Cory’s shearwater (Calonectris d. diomedea), 10 Atlantic Cory’s shearwater (Calonectris d. borealis) and one Cape Verde shearwater (C. edwardsii) breeding sites. Assignments rates were investigated at three spatial scales: breeding colony, breeding archipelago and taxa levels. Genetic analyses based on the mitochondrial control region (198 birds from 21 breeding colonies) correctly assigned 100% of birds to the three main taxa, but failed in detecting geographic structuring at lower scales. Discriminant analyses based on trace elements composition achieved the best rate of correct assignment to colony (77.5%). Body measurements or stable isotopes mainly succeed assigning individuals among taxa (87.9 and 89.9%, respectively) but failed at colony level (27.1 and 38.0%, respectively). Combining all three approaches (morphometrics, isotopes and trace elements on 186 birds from 15 breeding colonies), substantially improved correct classifications (86.0, 90.7 and 100% among colonies, archipelagos and taxa, respectively). Validations using two independent data sets and Jackknife cross-validation confirmed the robustness of the combined approach in the colony assignment (62.5, 58.8 and 69.8% for each validation test, respectively). A preliminary application of the discriminant model based on stable isotopes ?15N and ?13C values and trace elements (219 birds from 17 breeding sites) showed that 41 Cory’s shearwaters caught in the western Mediterranean longliners came mainly from Menorca (48.8%), Ibiza (14.6%) and Crete (31.7%) breeding colonies. Our findings show that combining analyses of trace elements and stable isotopes on feathers can achieve high rates of correct geographic assignment of birds in the marine environment, opening new prospects to the study of seabird mortality at sea.

(56) Trans-equatorial migration and mixing in the wintering areas of a pelagic seabird. Frontiers in Ecology and the Environment 6:297-301.

González-Solís, J., J.P.Croxall, D.Oro, & X.Ruiz (2007)

Despite the increasing interest in long distance migration, the wintering areas, migration
corridors and population mix in winter quarters of most pelagic marine predators are
unknown. Here we present the first study to track migration movements of shearwaters
throughout the complete nonbreeding period. We used geolocators (global location sensing
(GLS) units based on ambient light levels) to track 22 Cory’s shearwaters Calonectris
diomedea, breeding in three different areas. Most birds wintered in one or more of three
relatively small areas, all clearly associated with major coastal upwelling systems of the
tropical and south Atlantic. Transequatorial movements were dominated by prevailing trade
winds and westerlies and avoided calm oligotrophic areas. Breeding populations clearly
differed in their main preference amongst the three major wintering areas but showed
substantial mixing. This illustrates the exceptional value of GLS not only for describing and
determining the influence of oceanographic features on migration patterns, but also to
determine population mix in winter quarters. This knowledge is essential to assess the
impact of population level threats, such as longlining, offshore windfarms and oil spills, to
multiple breeding sites and ultimately critical in devising conservation policies that
guarantee the sustainable exploitation of the oceans.

A central point in life history theory is that parental investment in current reproduction should be balanced by the costs in terms of residual reproductive value. Long-lived seabirds are considered fixer investors, that is, parents fix a specific level of investment in their current reproduction independent to the breeding requirements. We tested this hypothesis analyzing the consequences of an experimental increase in flying costs on the foraging ecology, body condition and chick condition in Cory’s shearwaters Calonectris diomedea. We treated 28 pairs by reducing the wing surface in one partner and compared them with 14 control pairs. We monitored mass changes and incubation shifts and tracked 19 foraging trips per group using geolocators. Furthermore, we took blood samples at laying, hatching and chick-rearing to analyse the nutritional condition, haematology, muscle damage and stable isotopes. Eighty day old chicks were measured, blood sampled and challenged with PHA immune assay. In addition, we analysed the effects of handicap on the adults at the subsequent breeding season. During incubation, handicapped birds showed a greater foraging effort than control birds, as indicated by greater foraging distances and longer periods of foraging, covering larger areas. Eighty days old chicks reared by treated pairs were smaller and lighter and showed a lower immunity than those reared by control pairs. However, oxygen demands, nutritional condition and stable isotopes did not differ between control and handicapped birds. Although handicapped birds had to increase their foraging effort, they maintained physical condition by reducing parental investment and transferred the experimentally increased costs to their partners and the chick. This result supports the fixed investment hypothesis and is consistent with life history theory.

Cellular immune response (CMI) has been widely studied in relation to different individual and fitness components in birds. CMI is usually measured by tissue swelling resulting from T-lymphocyte production after injection of phytohemagglutinin-P. However, few studies have simultaneously examined individual, social factors and habitat-mediated variance in the immune response of chicks and adults from the same population and in the same breeding season. We investigated ecological and physiological variance in CMI response of male and female fledglings and adults in a breeding population of Cory’s Shearwaters, Calonectris diomedea, in the Mediterranean Sea. Explanatory variables included individual traits (body condition, carbon and nitrogen stable isotope ratios, ?15N and ?13C, plasma total proteins, triglycerides, uric acid, osmolarity and ß-hydroxy-butyrate, erythrocyte mean corpuscular diameter, hematocrit and hemoglobin) as well as burrow traits (temperature, isolation and physical structure). During incubation, immune response in adults was significantly greater in males than in females. Fledglings exhibited a lower immune response than adults. Ecological and physiological factors affecting immune response differed between adults and fledglings. GLM models showed that immune response in adult males was positively associated with burrow isolation, suggesting that males breeding at higher densities suffer a suppression of their immune system. In contrast, immune response in fledglings was positively associated with body condition and plasma triglyceride levels. Therefore adult immune response seems partly associated with social stress whereas in fledglings a trade-off between immune function and fasting capability may exist. Our results and those from previous studies, provide support for an asymmetric influence of ecological and physiological factors on the health of different age and sex groups within a bird population as well as for the importance of simultaneously considering individual and population characteristics in intraspecific studies of immune response.

We investigated phylogenetic relationships and the biogeographic history of the Calonectris species complex, using both molecular and biometric data from one population of the Cape Verde shearwater Calonectris edwardsii (Cape Verde Islands), one from the streaked shearwater C. leucomelas (western PaciWc Ocean) and 26 from Cory’s shearwater populations distributed across the Atlantic (C. d. borealis) and the Mediterranean (C. d. diomedea). The streaked shearwater appeared as the most basal and distant clades, whereas the genetic divergences among the three main clades within the Palearctic were similar. Clock calibrations match the Wrst speciation event within Calonectris to the Panama Isthmus formation, suggesting a vicariant scenario for the divergence of the PaciWc and the Palearctic clades. The separation between the Atlantic and Mediterranean clades would have occurred in allopatry by range contraction followed by local adaptation during the major biogeographic events of the Pleistocene. The endemic form from Cape Verde probably evolved as a result of ecological divergence from the Mediterranean subspecies. Finally, one Mediterranean population (Almeria) was unexpectedly grouped into the Atlantic subspecies clade, both by genetic and by morphometric analyses,pointing out the Almeria-Oran oceanographic front (AOOF) as the actual divide between the two Cory’s shearwater subspecies. Our results highlight the importance of oceanographic boundaries as potentially eVective barriers shaping population and species phylogeographical structure in pelagic seabirds.

(52) Stable isotopes reveal trophic segregation by sex and age in the southern giant petrel in two different foodwebs. Marine Ecology Progress Series 296: 107-113

We investigated trophic ecology variation among colonies as well as sex- and age-related differences in diet in the southern giant petrel (Macronectes giganteus), a long-lived seabird that is sexually dimorphic in size. We measured stable-isotopes (δ13C, δ15N) in the blood samples collected during breeding at Bird Island (South Georgia, Antarctica) in 1998 and at two colonies in the Argentinean Patagonia in 2000 and 2001. Individuals from South Georgia showed lower δ13C and δ15N values than in Patagonia, as expected from the more pelagic location and the short length of the Antarctic food web. Males and females showed significant differences in the isotopic signatures in both localities. These differences agree with the sexual differences in diet found in previous studies, which showed that both sexes rely mainly on penguin and seal carrion, but females also feed extensively on marine prey, such as fish, squid and crustaceans. However, in the Patagonia males showed significantly higher δ15N and δ13C values than females and the reverse trend was observed at South Georgia. This opposite trend is probably related to the different trophic level of carrion between locations: whereas penguins and pinnipeds in Patagonia rely mainly on fish and cephalopods, in South Georgia they rely mainly on krill. Stable isotope values of male and female chicks in Patagonia did not differ but both attained high values, similar to adult males but higher than adult females, suggesting that parents do not provision differentially their single offspring in relation to sex but instead provide higher proportion of carrion, probably a higher quality and more abundant food, than they consume themselves. Stable isotopes at South Georgia were not affected by age of adults. We have add new information on intraspecific segregation in the diet in a seabird species and also underline the importance of considering food web structure to study intraspecific variability in trophic ecology.

Bias in sex ratios at hatching and sex specific post hatching mortality in
size dimorphic species has been often detected, and is usually skewed
towards the production and survival of the smaller sex. Since common terns
show a limited sexual size dimorphism, with males being only about 1-6%
larger than females in a few measurements, we would expect to find small or
no differences in production and survival of sons and daughters. To test
this prediction, we carried out a two-year observational study on sex ratio
variation in common terns at hatching and on sex specific post hatching
mortality. Sons and daughters hatched from eggs of similar volume. Post
hatching mortality was heavily influenced by hatching sequence. In addition,
we detected a sex specific mortality bias towards sons. Overall, hatching
sex ratio and sex specific mortality resulted in fledging sex ratios 8%
biased towards females. Thus, other reasons than body size may be
influencing the costs of rearing sons. Son mortality was not homogeneous
between brood sizes but greater for two-chick broods. Since adults rearing
two-chick broods were younger, lighter and bred consistently later than
those rearing three-chick broods, it is suggested that lower capacity of
two-chick brood parents adversely affected offspring survival of sons.
Though not significantly, two-chick broods tended to be female biased at
hatching, perhaps to counteract the greater male-biased nestling mortality.
Thus, population bias in secondary sex ratio is not limited to strongly size
dimorphic species, but species with a slight sexual size dimorphism can also
show sex ratio bias through a combination of differential production and
mortality of sons and daughters.

We analyzed offspring sex ratio variation in Mediterranean Cory’s Shearwater (Calonectris d. diomedea) during two consecutive breeding seasons in two colonies. We test for differential breeding conditions between years and colonies looking at several breeding parameters and parental condition. We then explored the relationship between offspring sex ratio and parental condition and breeding parameters. This species is sexually dimorphic with males larger and heavier than females; consequently we expected differential parental cost in rearing sexes, or a greater sensitivity of male chicks to adverse conditions, which may lead to biased sex ratios. Chicks were sexed molecularly by the amplification of the CHD genes. Offspring sex ratio did not differ from parity, either at hatching or fledging, regardless of the colony or year. However, parental body condition and breeding parameters such as egg size and breeding success were different between years and colonies. Nevertheless neither nestling mortality nor body condition at fledging varied between years or colonies, suggesting that male nor female chicks were probably not differentially affected by variability in breeding conditions.

We investigated the possible effects of a 12-g data-logger attached to a darvic ring on the performance of Cory s shearwater (Calonectris diomedea, 600–850 g) from two different colonies in the western Mediterranean Sea. We compared return rates, current breeding success and body condition between equipped and unequipped birds. Effects on feeding ecology during winter and breeding period was also evaluated through the measurement of stable isotopes of carbon ( 13C) and nitrogen ( 15N) in one of the colonies. We found no evidence of negative effects of loggers on demographic parameters analysed or in feeding ecology. Power analyses suggested a high power to detect medium effect size, but a low power to detect small changes. Despite the non-significant results we could only exclude medium to strong effects of the devices on one of these parameters. We detected some short-term negative effects on body condition for the equipped birds, but these were unlikely to have had important consequences. Results suggest that the use of loggers is an adequate methodology to obtain information from seabirds at sea, but data should be carefully interpreted with regard to potential biases during severe environmental conditions.

The two sibling species of giant petrels, (northern Macronectes halli and the southern M.giganteus), the dominant scavengers of the subantarctic and Antarctic environment, are one of the best examples of sexual segregation in avian foraging and feeding ecology. During breeding, males and females feed mainly on penguin and seal carrion, but females also feed extensively on marine prey such as cephalopods, fish and krill. Sexual differences in diet are reflected not only in analyses of regurgitations, but also in the isotopic composition of carbon and nitrogen, as well as in their heavy metal burdens. Direct observations and tracking of pelagic movements showed that males of both species usually forage closer to the breeding grounds, exploiting carcasses on beaches, whereas females show more pelagic habits. In consequence, foraging effort, foraging efficiency, predictability of resources exploited, optimum foraging time and activity budgets differ between sexes. During winter, however, studies on activity and pelagic movements suggest more similar feeding habits between sexes. Overall, differences in foraging and feeding ecology are probably related to the substantial sexual size dimorphism; males of both species are 16-35% heavier and have disproportionately larger bills than females. The importance of size in contest competition to access carrion may explain their large body size as well as the competitive exclusion of females from coastal habitats, reducing intraspecific competition for food. Ultimately, a differential exploitation of carrion probably increased sexual dimorphism not only in body size but also in the feeding structures such as bill size. The apparently greater profitability of the feeding strategies of males compared to females suggests that giant petrels reflect an evolutionary process that benefits each sex differently.

BACKGROUND AND INTRODUCTION
Seabirds belonging to the order Procellariiformes (albatrosses and petrels) are amongst the most pelagic of seabirds and occur in all of the world’s oceans. They are, therefore, excellent potential indicators of the state of marine ecosystems, especially high seas. The status and trends of albatross breeding populations are well documented and, with 19 of 21 species now globally threatened and the remainder Near Threatened (BirdLife International 2004a); albatrosses have become the bird family most threatened with extinction. Many petrel species are also globally threatened. Although albatross and petrel species face many threats at their breeding sites, the main problems they encounter currently relate to the marine environment, particularly involving interactions with fisheries, notably the many thousands of birds killed annually by longline fishing. Many of the solutions to these problems require accurate knowledge of the distributions of albatrosses and petrels throughout their annual and life cycles. Such data are also invaluable for understanding many aspects of the ecology and demography of these species and their role in the functioning of marine systems—including their potential susceptibility to changes in these. In terms of remote-tracking to reveal their at-sea distribution (a key to understanding how they function in marine ecosystems), albatrosses (and giant-petrels) are the most studied of all marine species. Given the substantial potential of these data for conservation applications, including for marine analogues of terrestrial Important Bird Areas (IBAs), pioneered by BirdLife since the 1980s, BirdLife convened an evaluation workshop to explore the data and concepts with the main dataholders. This report presents the results of the workshop.
AIMS
The main strategic aims of the workshop were:
1. To assess how at-sea distribution data from remotetracking studies of seabirds can contribute to: i. the development of criteria for defining Important Bird Areas (IBAs) in the marine environment; ii. current initiatives for the establishment of high seas Marine Protected Areas (MPAs) especially by IUCN.
2. To scope the extent to which these data can be used to quantify overlap between marine areas used by albatrosses and the location of fishing effort, especially longline: i. to identify areas of higher risk, especially for the development of appropriate mitigation measures for the fisheries involved; ii. to identify the Regional Fishery Management Organisations (RFMOs) with prime responsibility for the management of fisheries with significant risk of incidental bycatch of globally threatened nontarget species, especially albatrosses and petrels.
3. To establish a Geographic Information System database to maintain detailed information on remote-recorded range and distribution of seabirds, as an international conservation tool.
RESULTS
Data and methods
• Over 90% of all extant albatross and petrel tracking data was submitted to the workshop, representing 16 species of albatross, both species of giant-petrel and Whitechinned Petrel. A GIS database was developed to facilitate analysis, visualisation and interpretation of these data. • Standard analytical procedures were developed and applied to the satellite tracking (PTT) data from raw data records submitted by dataholders. • Consistent procedures were developed for the presentation of geolocator tracking (GLS) data—the main source of information for distributions in nonbreeding seasons.
• Appropriate analytical procedures were agreed for transforming location data into density distributions, a crucial step in the visualisation, analysis and interpretation of multiple data sets.
• Protocols for data access and use, acknowledging the need to make available information to the international conservation community while safeguarding the proprietary rights of the individual data contributors and data users, were agreed.
Analysis and case-histories
The data available allowed the demonstration of a variety of properties relating to albatross and petrel ecology and distribution, including:
• The nature and variation in range and distribution, for breeding birds, in relation to stage of breeding season, gender (sex) and year (i.e. interannual variation).
• Differences in range and distribution of breeding birds from different colonies within the same population (island group).
• Similarities and differences in range and distribution of breeding birds from different populations of the same species, using data for the two species (wandering albatross, black-browed albatross) with the most comprehensive data, which provide compelling evidence of the insights that can be generated by applying common and consistent approaches to data from a variety of studies and sites.
• Regional syntheses for providing clear indications of the potential (and challenges) for using data across a range of albatross and petrel species to identify areas of key habitat common to different species.
• Similarities and differences in range and distribution of breeding and non-breeding birds at the same time of year.
• The spectacular journeys and far-distant destinations (comprising migratory routes, staging areas and wintering ranges) of some species of albatross and petrel during the non-breeding season. These represent very significant achievements, some indicating interesting aspects and avenues for future research, others identifying potential biases and concerns relating to analysis and interpretation of data, yet others revealing key gaps in viii our knowledge. Nevertheless, all indicate the potential of such data to address important questions relating to albatross and petrel ecology and conservation.
Strategic aims and applications
Definition of Important Bird Areas (IBAs) and contribution to high seas Marine Protected Areas
• Tracking data for albatrosses and petrels will make a key contribution to attempts to identify areas of critical habitat for marine organisms and hotspots of biodiversity in coastal and pelagic marine ecosystems.
• Characterising density distributions and combining (weighting) these with estimates of source population size, will be fundamental approaches for marine taxa.
• The extent to which existing definitions of IBAs, developed for terrestrial species and systems, can be extended to marine contexts requires considerable further investigation for which the albatross and petrel data are uniquely suited; however, approaches which combine data from different groups of marine animals (e.g. fish, seabirds, marine mammals) are likely to be essential in longer-term
approaches to issues of marine habitat conservation.
• The albatross and petrel data represent a uniquely coherent and comprehensive data set, covering large areas of marine habitat, and are therefore especially suitable for further investigation, perhaps particularly in high seas contexts.
Interactions with fisheries and fishery management organisations
• Examples of overlap between albatross distribution (both breeding and on migration) and fishing effort illustrate the considerable importance and potential of approaches to match data on the distribution (and abundance) of albatrosses and petrels with data on
fishing effort, particularly for longline fisheries.
• Difficulties in obtaining data for appropriate scales and times, even for the better documented fisheries, may constrain what can be achieved, especially in terms of analysis seeking to estimate bycatch rates and/or their impact on source populations of albatrosses.
• Nevertheless, even existing data are adequate to provide broad characterisation of the location (and timing) of potential interactions between albatross species and different longline fisheries; this is a high priority task.
• These data are used to provide a preliminary identification of the responsibilities of RFMOs for environmentally sensitive management of albatrosses and their habitat based on overlap of ranges and jurisdictions. For the Southern Hemisphere this provides very clear indications of the critical role of, in preliminary priority order, Commission for the Conservation of Southern Bluefin Tuna (CCSBT), Western and Central Pacific Fisheries Commission (WCPFC), Indian Ocean Tuna Commission (IOTC), International Commission for the Conservation of Atlantic Tunas (ICCAT) and Commission for the Conservation of Antarctic Marine Living Resources (CCAMLR).
• A similar initial review, in relation to Exclusive Economic Zones (EEZs), is also provided.
• Combined with data on overlap with fishing operations, these will enable preliminary identification of the times, places and fisheries where adverse interactions are most likely and, thereby, allow the identification of mitigation measures appropriate to the circumstances.
Maintaining the database as an international conservation tool
Participants agreed to maintain the tracking database, assembled for the purposes of this workshop, beyond the meeting and production of its report.
• The database should be maintained and reconstituted by re-submission of data on the basis of the agreed policy on data access and use.
• A policy and practice for data access and use (based on principles developed for the Census of Marine Life Ocean Biogeographic Information Service (OBIS) – SEAMAP Programme) was agreed.
• BirdLife International offered, at least as an interim measure, to house and manage the database at its Secretariat headquarters in Cambridge, UK.
• The offer was accepted in principle. However the need to maintain and augment the data, to facilitate interactive and collaborative use, to link the albatross and petrel and tracking data to other, analogous, data sets and to the latest information on the physical and biological marine environment was recognised. Possibilities for linking, or possibly migrating, the Procellariiform Tracking Database from BirdLife to an organisation or institution specialising in the management and analysis of data on marine systems and biogeography should be investigated.
FUTURE WORK
Existing data
• All data submitted to the workshop should be resubmitted to the new database, managed by BirdLife, and subject to the agreed data access and use procedures.
• Other extant data, especially for Antipodean and Waved Albatrosses, and Westland Petrel Procellaria westlandica, should be requested from relevant dataholders and data owners.
• New data should be requested as it becomes available.
New data
Priorities are:
• For breeding birds, more data (and in most cases from more individuals) are needed for some stages of the breeding cycle (particularly incubation), for sexed birds and for sufficient years to assess the consistency of basic distribution patterns, for additional populations (island groups) and from more colonies within populations.
• For most species, data on the distribution of adults when not breeding.
• For almost every species, data on the distribution of immatures and early life-history stages.
Methods
• Evaluation of the potential biases of using the different types (and where appropriate different duty cycling) of existing data (e.g. PTT, GLS) in different kinds of analysis and on the use of appropriate spatial statistics to create density distributions from the different kinds of tracking data.
Environment
• Need to facilitate easy access to appropriate data sets on the physical and biological environment at appropriate scales, including detailed bathymetry, sea surface temperature, marine productivity, sea-ice etc. Tracking ocean wanderers: the global distribution of albatrosses and petrels – Executive summary
Links to other tracking/sighting data on pelagic marine taxa
• Facilitate links to analogous sets of data on other petrels, penguins, marine mammals, sea turtles and migratory fish.
• Encourage and support links with initiatives like the Marine Mammal Tracking Database and programmes like the Census of Marine Life’s Tagging Of Pacific Pelagics which are trying to assemble similar data on a collaborative basis.
• Investigate the feasibility and utility of combining remote tracking and survey data sets. Prime candidate areas for pilot studies to do this with seabird data would include the north-east Pacific, tropical east Pacific, south-west Atlantic and parts of the Indian Ocean.
Links to data from fisheries
Compare and analyse the distribution data for albatrosses/ petrels and fishing effort to:
• Identify times and places where potential exists for adverse interactions between fisheries and albatrosses/ petrels. This would enable: i. Specification of mitigation measures appropriate to these circumstances; ii. Approaches to RFMOs with appropriate jurisdictions, singly or in combination, to seek to develop the necessary regulations to apply the mitigation measures.
• Estimate bycatch rates of albatrosses/petrels for appropriate areas and at appropriate scales and for extrapolation to areas where bycatch data from fisheries are currently lacking.
• Assist modelling of seabird-fishery interactions with implications for fisheries (taking financial losses through
bycatch into account in cost-benefit analyses) and for seabird populations.
IBAs and Marine Protected Areas
• Identify and relate areas of core habitat to population estimates and threatened status to evaluate in detail the implications of different criteria for helping define marine IBAs.
• Develop this approach further by choosing suitable systems/areas in which to link to remote-tracking data on other seabirds (especially penguins) and to at-sea survey data. This is especially relevant for coastal and shelf systems (i.e. within EEZs).
• Develop this further relative to Marine Protected Areas in conjunction with data on other marine taxa (e.g.marine mammals, sea turtles) and on resource use (e.g. fisheries, hydrocarbons). This is relevant both to EEZs and to high seas.
Agreement on the Conservation of Albatrosses and Petrels (ACAP)
• The applications envisaged of the albatross and petrel tracking database are highly relevant to the conservation aims of ACAP. The database is likely to be a key tool for furthering the work of ACAP.

It has been suggested that breeding performance differs between young and old birds due to the appearance and disappearance of phenotypes through differential survival (selection hypothesis) or differential recruitment (delayed breeding hypothesis) of high-quality individuals, but each bird may show constant breeding performance over its life. We tested constant egg-volume and laying date by modelling their variability on the basis of the 109 known-age females of common tern Sterna hirundo with data available from 1 to 9 years. Longitudinal analyses showed a significant advancement of laying date, as well as a steady increase in egg-volume, in young age classes from 2 to 5–7 years old, indicating individual intrinsic changes in performance with age. In our model, female effect accounted for 74% and 8% of variance in egg-volume and laying date, respectively, suggesting that if correlation between breeding performance and survival or recruitment exists, population patterns of age-specific performance may emerge. However, we found no evidence that birds that did not return to breed during young age classes laid later or smaller eggs than returned breeders. Likewise, we found no evidence that recruiting birds laid earlier or larger eggs than same aged birds recruited in preceding years. Thus, this study shows that age-specific patterns in timing of breeding and egg-size in common terns result from individuals intrinsic changes, and we reject the selection and the delayed breeding hypotheses as a major factor shaping age-specific patterns at population level.

Northern giant petrels (Macronectes halli) are among the largest and most sexually size dimorphic species of seabirds, with females being only 80% the mass of males. Both sexes scavenge on seal and penguin carrion in the sub-Antarctic ecosystem, but during the breeding season females also feed extensively on other marine food resources and show more pelagic habits than males. The outstanding sexual segregation in foraging and feeding ecology in northern giant petrels suggests that mechanisms maintaining sexual size dimorphism by ecological factors may be operating. I evaluated this possibility by examining ecological correlates with body size and by static allometry analyses. Proportion of male fledglings was 0.36 (n=33 fledglings). There was no assortative mating by size neither association between the male size with the breeding performance. By contrast, smaller females raised their chick in better condition. Moreover, bill size showed a size dimorphism beyond that expected by body size dimorphism, i.e. when controlling for body mass, males showed relatively longer bill than females. This trait did not deviate from isometry with respect to body size and its phenotypic variability was low, suggesting that the disproportionately large bill of males is related to their more scavenging life style compared to females. In general, the increase and maintenance of sexual size dimorphism in giant petrels is more consistent with an ecological causation rather than a result of sexual selection.

In most seabird species incubation shifts shorten when hatching approaches, a behavioural response allowing the chick to be fed soon after hatching. Three mechanisms have been proposed to explain these shortened absences: endogenous timing; a response to embryo signals or, a seasonal increase in food availability. I tested these hypotheses by cross-fostering eggs between two sibling species: the northern Macronectes halli and the southern M.giganteus giant petrels, the former normally breeding 5-6 weeks earlier than the latter but both have an incubation period of 60 days. The length of the last trip before hatching was unaffected by the timing of hatching, suggesting that trip length was not related to the increase in food availability with the progress of the season. Moreover, northern giant petrels reduced their last trip lengths regardless of whether or not they were incubating their own developed or the southern undeveloped egg (with embryos not developed enough to signal). Parents presumably, therefore, possess endogenous control allowing them to predict hatching date and to reduce trip duration accordingly, regardless of the communication with the embryo. Nevertheless, shorter last trips before hatching in southern giant petrels incubating northern giant petrel eggs suggest that embryo signals are used by parents for a fine tuning synchronisation of the internal timer to the natural variability in the length of the incubation period.

The concentrations of PCBs, DDTs, HCHs, HCB and OCS were determined in sediments and associated biota, both invertebrates (Physella acuta, Hirudo medicinalis, chironomid larvae, Hydrous pistaceus, Helochares lividus) and vertebrates (Rana perezi), in a temporary aquatic system, a rice-field in the Ebro Delta (NE Spain). The qualitative and quantitative distribution of organochlorine compounds in sediments and aquatic biota has been explained by two mechanisms: equilibrium partitioning and/or biomagnification through the trophic web. Nevertheless, bioaccumulation processes are by far more complex, since several biotic and abiotic factors contribute to the observed pollutant loads in the organisms. In this respect, the biological characteristics of the organisms considered (e.g. species, age, lipid contents, feeding habits, etc.), as well as ecological factors (e.g. the habitat of the species and vertical distribution), have been shown to account for the organochlorine levels observed.

Black rat Rattus rattus populations can reach high densities in the Mediterranean islands, as it has been the case in Chafarinas Islands (Western Mediterranean coast) in the last decade. This archipelago holds the second largest breeding population of Audouin's gull Larus audouinii and an important population of yellow-legged gull Larus cachinnans. Circumstantial evidences of rat predation upon Audouin's gull eggs led to conservation concerns over the impact of rat predation on gull productivity. We studied the impact of rats on gull nests experimentally by building artificial nests into the breeding area of yellow-legged gulls. Two kinds of eggs were placed in the artificial nests: manipulated eggs, in which the shell was previously opened, and entire (unmanipulated) eggs. Rats preyed extensively on opened eggs but only once on entire eggs, apparently due to lack of skills to prey on entire eggs, suggesting that rats do not normally have access to fresh gull eggs. Rats inhabiting breeding areas of gulls can scavenge on broken eggs rolled out of the nest or on eggs with softened shells after nest desertion, but it is unlikely that they prey on natural gull nests guarded by parents. We suggest that introduced black rats in Mediterranean islands can only marginally affect the productivity of gulls owing to egg predation.

(41) Kleptoparasitism, disturbances and predation of yellow-legged gulls on Audouin’s gulls in three colonies of the western Mediterranean. Scientia Marina 67 (Suppl 2): 89-94.

The impact of Yellow-legged gulls on Audouin's gulls was studied through observations from a blind, at the Ebro Delta, the Chafarinas Islands and the Columbretes Islands colonies, during different stages of the Audouin's gull breeding season. The rates of predation (upon eggs and chicks) and kleptoparasitism (aerial and on courtship and chick-feeding regurgitates) were recorded to assess this impact. Kleptoparasitism and predation rates recorded at the three colonies may be considered low when compared with similar studies dealing with other gull species. It seems that the interactions did not represent a threat to population dynamics of Audouin’s gulls in any of the three colonies. The highest rates of aerial kleptoparasitism, courtship feeding and chick mortality were recorded at the Columbretes Islands, whereas the lowest at the Ebro Delta. The ratio of number of Yellow-legged gulls to number of Audouin’s gulls seems to influence the rate of disturbances. Food availability is also likely to play an important role in the number of interactions, which increased when food was in shorter supply. The study at the Ebro Delta and Chafarinas was restricted to some subcolonies and results may not be necessarily representative of the entire colonies. The existence of some Yellow-legged Gull individuals specialized as predators were recorded. Finally, we discuss the suitability of several conservation measures commonly applied in colonies where both species breed syntopically.

Interactions between yellow-legged Larus cachinans and Audouin's Larus audouinii gulls and fisheries operating around the Chafarinas Islands, located 4.5 km off the Moroccan Mediterranean coast, are reviewed. At the Chafarinas archipelago two distinct types of fisheries operate: trawlers and purse seines. Gulls take advantage of both fisheries. They scavenge trawler discards and congregate around shoals of fish attracted to the surface by the purse-seine lamps. When both trawlers and purse-seine boats are in operation, the diet of both gull species is similar, with epipelagic fish accounting for over 60% of the biomass, partially collected in association with the purse-seine fishery. When only trawlers operated yellow-legged gulls, but not Audouin's gulls, augmented their diet mainly with human waste from refuse dumps, suggesting that competition for food between the two species is mainly limited to the periods when resources made available by fishery activities are abundant. Likewise, when only trawlers operated, there was an increase in the predation pressure on eggs and chicks of Audouin’s gulls. In particular, during the week of celebrations for the holy lamb festival when neither fishery operated, egg losses of Audouin’s gull increased dramatically, suggesting that severe food shortage caused by the cessation of fishing can result in an increased predation pressure by yellow-legged gulls, affecting Audouin’s gull productivity. These results suggest a novel socio-ecological link between gulls, fisheries and local feasts.

We analysed concentrations of cadmium, lead, mercury and selenium in blood from males and females of the 2 sibling species of giant petrels, the northern Macronectes halli and the southern M. giganteus, breeding sympatrically at Bird Island (South Georgia, Antarctica). Blood samples were collected in 1998 during the incubation period, from 5 November to 10 December. Between species, cadmium and lead concentrations were significantly higher for northern than for southern giant petrels, which probably resulted from northern giant petrels wintering in more polluted areas (mainly on the Patagonian shelf and Falkland Islands) compared to southern giant petrels (wintering mainly around South Georgia and the South Sandwich Islands). Between sexes, cadmium concentrations were significantly higher for females than for males in both species, corresponding to the more pelagic habits of females compared to the more scavenging habits of males. Lead and cadmium concentrations in circulating blood decreased significantly over the incubation period, suggesting that when breeding at Bird Island, exposure to the source of pollution had ended, and these metals had been cleared from the blood and excreted, or rapidly transferred to other tissues. Association of lead and cadmium with a common source of pollution was further corroborated by a significant positive correlation between the levels of the 2 elements found. Mercury levels were similar between the species, but showed an opposite trend between sexes, with males showing higher levels than females in northern giant petrels, and the opposite was true in southern giant petrels, with no changes throughout incubation. Selenium levels were similar between sexes, but significantly greater for northern than for southern giant petrels. Moreover, there was a significant increase in the selenium levels over the incubation period in northern giant petrels. Age of adult birds did not affect metal concentrations. Coefficients of variation of metal levels were consistently lower for northern than for southern giant petrels, particularly for mercury, suggesting that the former species is more dietary specialised than the latter. Contaminant analyses, when combined with accurate information on seabird movements, obtained through geolocation or satellite tracking, help us to understand geographic variation of pollution in the marine environment.

We studied the mercury contamination of 13 species of seabirds breeding on Bird Island, South Georgia, in 1998. Total mercury concentrations in body feather samples of birds caught at their breeding colonies were determined. Among the species, Grey-headed Albatross (8,933 ng g-1) and Southern Giant Petrel (7,774 ng g-1) showed highest, and Gentoo Penguin (948 ng g-1) lowest, body feather mercury concentrations. Mercury levels were negatively correlated with the proportion of crustaceans (mainly krill) in the species’ diets, suggesting that the trophic level is the most important factor in explaining the variation of mercury concentrations in Antarctic seabirds. In four species studied for age effects among adult birds (Grey-headed and Black-browed Albatross, Northern and Southern Giant Petrel), no age dependent variation in mercury levels was found. Sex differences were also assessed: female Gentoo Penguins had lower mercury levels than males, which may be related to the elimination of part of the mercury body burden by females into eggs. In contrast, Northern Giant Petrel males had lower levels than females, which may be related to a higher consumption by males of carrion from Antarctic fur seals. In Grey-headed Albatrosses, mercury levels were 113% higher than 9 years before (1989) when this species was investigated at the same site, indicating a possible increase in mercury pollution of the Southern Ocean during the last decade.

(37) Mounting frequency and number of cloacal contacts increase with age in common terns Sterna hirundo. Journal of Avian Biology 33: 306 - 310.

González-Solís, J. & P.H.Becker (2002)

We investigated courtship feeding and copulatory behaviour of common terns Sterna hirundo in relation to age. We found a significant positive correlation between the total number of courtship feedings delivered to females and the total number of mountings. Moreover, both courtship feeding and mounting rates were positively correlated with female and male age. Also, the number of cloacal-contacts per mounting increased significantly with male or female age, which may indicate an increase in copulatory ability with age. The number of mountings for older birds was up to 3 times the number for younger birds. Presuming that transfer of sperm is proportional to the number of cloacal contacts, older birds may have transferred up to 6 times more sperm than younger birds during the pre-laying and laying period. These results may account for the preference of young females for older mates as well as for older extra-pair mates.

(36) Differences in diet between the two largest breeding colonies of Audouin's gull: the effects of fishery activities. Scientia Marina 66: 313 - 320.

Pedrocchi, V., D.Oro, J.González-Solís, X.Ruiz & L.Jover (2002)

We analysed and compared the diet of Audouin's gulls Larus audouinii between their two largest breeding sites in the world: the Ebro Delta and the Chafarinas Islands (northwestern Mediterranean). These two localities showed marked differences in the features of commercial fishing fleet: in the Ebro Delta area a large fishing fleet produced large amounts of discards, while in the Chafarinas the fleet discarded smaller amounts of fish and marine invertebrates, due to the lower number of vessels. It is also likely that percentage of discards from total catches is also lower around the Chafarinas than at the Ebro Delta. We distinguished two types of fishing to compare diet compositions: diurnal (only trawling activity) and diurnal and nocturnal (trawling and purse-seine activity, respectively). We also differentiated regurgitates from young nestlings (up to 20 days old) and from older nestlings or adult birds. At the two localities, fish was the main food of Audouin's gulls, with epipelagic prey (mainly clupeoids) being more important when both diurnal and nocturnal fisheries were operating. This confirms that epipelagic prey either caught actively by the gulls or linked to fisheries was particularly important in the feeding habits of Audouin's gulls. Nevertheless, differences between the two colonies appear mainly when only trawlers operated: while at the Ebro Delta gulls showed higher consumption of benthic-mesopelagic prey (probably linked to a higher trawler discard availability), gulls from the Chafarinas Islands consumed higher biomass of epipelagic prey probably caught actively by night. When both fleets operated around the two colonies, the average biomass of prey in a regurgitate of younger chicks was significantly higher at the Ebro Delta than at Chafarinas, and the opposite trend was recorded for older nestlings and adults. Niche width was broader in Chafarinas than in the Ebro Delta for both age classes and for any fishing fleet schedule, suggesting again that the exploitation of discards was higher at the Ebro Delta than at the Chafarinas, where gulls show a more varied diet. Despite that availability of discards was probably higher at the Ebro Delta than at Chafarinas, the per capita availability was not so different at both localities due to the increasing seabird community population at the Ebro Delta, which ca. doubled that at Chafarinas in the last decade.

We studied foraging activity of giant petrels during the incubation period, by simultaneously deploying activity recorders and satellite transmitters on northern (Macronectes halli) and southern giant petrels (Macronectes giganteus) at Bird Island (South Georgia, Antarctica) between 29 October and 26 December 1998 . Satellite tracking showed two types of trips: (1) coastal trips, all undertaken by male northern giant petrels, to the nearby South Georgia mainland, presumably foraging on seal and penguin carcasses on beaches, and (2) pelagic trips, foraging at sea for marine prey or potentially scavenging on distant archipelagos (e.g. South Sandwich, Falkland or South Orkney Is.). Activity recorder data were consistent with the types of trip defined by the satellite tracking data, with median wet activity (time spent at the sea surface) during pelagic trips being 41%, but only 14% on coastal trips. On pelagic trips, there was a significant negative correlation between the duration of wet periods and the speed of travel between satellite uplinks. Mean travelling speed between uplinks was greater during day than night for both types of trips, suggesting that giant petrels prefer to travel during daylight and are less active at night. The scarcity of wet periods during the night in giant petrels foraging to the South Georgia coast (median 3%, range 1-9 %) indicates that such birds spent almost all night on land. Likewise, the scarcity of wet periods at night for three birds foraging 700-1000 km south of Bird Island, where there is no land but abundant icebergs, suggests these birds were resting on the icebergs at night. In addition to the adaptations to scavenging on carrion, pelagic trips by giant petrels contain elements similar to those of albatrosses, indicating a complexity to giant petrel lifestyle hitherto unrecognised.

Over the breeding season, courtship feedings in pairs of common terns were estimated to range from 0 to over 150 and copulations from 0 to over 50 per pair, based on a daylength of 17 h. The numbers of both were negatively correlated with laying date, suggesting that late breeding was a consequence of poor-quality males unable to provision their females adequately. Although there was not usually a temporal link between courtship feedings and copulations, overall they were strongly correlated, suggesting that females copulate repeatedly with the same male to gain food. High food availability, as indicated by low tide, was associated with high levels of courtship feeding and few copulations, and vice versa, whereas we did not detect any pattern associated with the time of day. Three of 272 courtship feedings, and two of 76 copulation attempts, were extrapair. Only one of 34 broods contradicted the assumption that broods contained only true siblings, in accordance with the high levels of sexual fidelity observed.

(31) Population density of primates in a large fragment of Brazilian Atlantic forest. Biodiversity and Conservation 10: 1267-1282.

González-Solís, J., J.C.Guix, E.Mateos & L.Llorens (2001)

We performed a line transect survey (352.4 km) of primates in the Serra de Paranapiacaba, at one of the largest relatively undisturbed fragments of the Atlantic rainforest of Southeastern Brazil (ca. 1400 km2), in August 1998. The brown capuchin, Cebus apella nigritus, was the most common species found in the area (20 groups, density estimate: 5.31+-2.05 individuals per km2, mean+-SE). Nine groups of the brown howler monkey, Alouatta guariba clamitans, and eight of the woolly spider monkey, Brachyteles arachnoides arachnoides, were also recorded, with preliminary density estimates of 0.79+-0.40 and 2.33+-1.37 individuals per km2, respectively. Density estimates for these species in other fragments of Atlantic rainforest are reviewed, showing that densities in Paranapiacaba are among the lowest reported. It is suggested that the higher densities reported for isolated populations in small forest patches (<50 km2) is related to the absence of main primate predators, the density compensation phenomenon and the ecological plasticity of some primate species. In contrast, local extinction in many small patches is probably related to hunting pressure. Given the important primate populations found in the Paranapiacaba fragment, conservation strategies for the studied species should give priority to effective protection of the largest remnant fragments from illegal hunting and deforestation, rather than translocation of individuals or captive breeding programs to introduce monkeys in small forest fragments vulnerable to hunting and of uncertain future.

From 1992 to 1999, we used subcutaneously injected transponders, enabling remote and automatic annual identification with the aim of performing an integrated population study of a Common Tern Sterna hirundo colony. Within a few years, population parameters and their inter-year variation could be calculated with a high degree of accuracy (adult return rate 91%, subadult return rate 35%, breeding success 1.3 fledglings pair-1y-1, mean recruitment age 3y). Data on condition, fate, reproductive performance and output were gathered year by year and contributed greatly towards the detailed study of many questions in behavioural and population ecology, such as individuals' quality, prospecting and recruitment. The arrival time of prospectors and recruitment age were related to body condition. The high body mass of adults increased their reproductive performance and output. Divorce rate was low (18.9%) and increased with arrival asynchrony of mates. Divorced terns tended to change territories, whereas territory fidelity in general was high. The future focus of this ongoing study is reproductive value, senescence, lifetime reproductive success, fitness, and questions related to genealogy.

During the Audouin's Gull's breeding season at the Ebro Delta in 1993, 24 fresh eggs from eight three-egg clutches (modal clutch-size) were collected at the peak of the laying period. Eggs were processed to obtain formalin-fixed yolks, which were halved and stained using the potassium dichromate method. Digitized images of the yolks were examined to assess the daily rates of yolk deposition. We used these data in combination with egg compositional analysis to build a model of energy demands during the formation of an average clutch in Audouin's Gull. To show how the different parameters of clutch formation affect the daily energy investment peak, we performed a simulation analysis in which the rapid yolk development (RYD) period, the follicle triggering interval (FTI), the laying interval (LI) and the albumen synthesis period (ASP) were allowed to vary simultaneously. In our sample, the mean RYD period was seven days with a range from six to eight days. There were no significant differences in yolk volume among eggs in a clutch, but albumen volume was significantly smaller in third eggs. According to our model the albumen synthesis of the a-egg coincides with the energy demand peak for clutch formation. This peak represents an increase by ca. 42% in female energy requirements. Values obtained from the simulation analysis showed that only the ASP of the a-egg and the RYD durations of the second and third follicles produced noticeable reductions in peak energy investment. We predict that in gulls, whose laying intervals seem to be kept constant, significant increases of the durations of the RYD periods of second and third eggs, or even significant reductions of yolk size of these eggs, may operate simultaneously to match the energy demands during clutch formation to the prevailing food conditions.

(27) Foraging partitioning between giant petrels Macronectes spp and its relationship with breeding population changes at Bird Island, South Georgia. Marine Ecology Progress Series 204: 279-288.

González-Solís, J., J.P.Croxall & A.G.Wood (2000)

We satellite-tracked the foraging trips of males and females of the 2 sibling species of giant petrels, Macronectes halli and M. giganteus, breeding sympatrically at Bird Island (South Georgia, Antarctica), during the incubation period (November-December). Size of the activity range in addition to speed and distance covered on foraging trips were similar between the species, but were lower for males than for females in both species. Sex-specific differences agree with previous observations on diets and on attendance at seal carcasses, suggesting that females mainly forage at sea, whereas males mainly scavenge on the coast. Overall, however, the foraging ecology of both species seems very similar. Interspecific and intersexual competition may be reduced by the limited overlap in the at-sea range, with southern giant petrels foraging further south than did northern giant petrels, and females further west than males, suggesting some spatial partitioning in foraging areas. Male northern giant petrels foraged almost exclusively on the South Georgia coast; their strong dependence during the brooding and chick-rearing period on Antarctic fur seals, whose population has increased exponentially in recent years, may be reflected in the recent population increase of northern giant petrels at South Georgia. Foraging areas of giant petrels overlapped extensively with longline fishery distribution, highlighting their susceptibility to being caught on longline hooks. Females were at higher risk during the study period since they made longer trips and foraged further west than males, into areas where local longline fisheries are more active.

Giant petrels (Macronectes spp.) are the most sexually dimorphic of all seabirds. We used satellite-tracking and mass change during incubation to investigate the influence of sexual size dimorphism, in terms of the intersexual food competition hypothesis, on foraging and fasting strategies of northern giant petrels at South Georgia. Females foraged at sea whereas males foraged mainly on the South Georgia coast, scavenging on seal and penguin carcasses. Foraging effort (flight speed, distance covered, duration of foraging trips) was greater for females than for males. In contrast, foraging efficiency (proportionate daily mass gain while foraging) was significantly greater for males than for females. Females were significantly closer to the desertion mass threshold than males and could not compensate for the mass loss during the incubation fast while foraging, suggesting greater incubation costs for females than for males. Both sexes regulated the duration and food intake of foraging trips depending on the depletion of the body reserves. In males the total mass gain was best explained by mass at departure and body size. We suggest that sexual segregation of foraging strategies arose from size-related dominance at carcasses, promoting sexual size dimorphism. Our results indicate that sex-specific differences in fasting endurance, contest competition over food and flight metabolic rates are key elements in maintenance of sexual size dimorphism, segregating foraging strategies and presumably reducing competition between sexes.

In long-lived seabirds with low annual reproductive output, the renesting decision after breeding failure is critical, and the parents have to weigh benefits of replacement clutches against possible future reproductive costs. In this study, we investigated factors influencing renesting decisions in common terns (Sterna hirundo) and compared aspects of breeding biology and body mass between two breeding attempts by the same pairs in each of 4 years of heavy losses due to predation. Renesting birds were characterized by early laying dates and by a high age. Among early breeders, high egg mass reduced the probability of renesting. A long relaying interval coincided with low mass of replacement eggs in one year, and short intervals with high egg mass in another. Further, egg mass decreased and relaying intervals increased the later the predation events occurred. Evidence of high levels of parental care of replacement clutches came from body mass data: female mass increase prior to egg laying was higher in the second attempt than in the first, whereas male mass was lower during the second courtship period than during the first. Male mass also affected relaying intervals and mass of replacement eggs. We conclude that common terns expend high levels of parental care of replacement clutches. Intrinsic factors related to individual quality (age, body condition) seemed most important for renesting decisions and for the degree of parental care, but foraging conditions seemed to have modifying effects.

(24) Variation in the diet of the Viperine Snake, Natrix maura, in relation to prey availability. Ecography 23: 185-192.

Santos, X., J.González-Solís & G.A.Llorente (2000)

The diet of the viperine snake was compared with food availability in the Ebro Delta, a wetland largely occupied by rice fields, in 1990 and 1991. Snake selection of prey type and size was studied seasonally and by snake group: males, females and immature snakes. Overall, feeding activity (percentage of individuals with prey and number of prey per stomach) increased with food availability. Diet analysis showed that viperine snakes mainly foraged on the green frog Rana perezi (adults and tadpoles) and the carp Cyprinus carpio. Conversely, viperine snakes rejected the mosquito fish Gambusia holbroki which is the most abundant species in autumn, when Natrix maura has a low feeding activity. Statistical comparisons between viperine snake diet and prey availability showed that males selected small carp, immature snakes selected tadpoles and, in spring, females selected frogs. The selection of small carp by males may reflect a sexual divergence of trophic niche related to sexual size dimorphism, as females are larger than males. As tadpoles are presumably easier to catch than fish, tadpole selection by immature individuals may reflect variance in capture abilities. In spring, the selection of frogs by females overlapped with vitellogenesis, suggesting that females compensate for the cost of reproduction by selecting green frogs, which have a greater biomass and higher energy content than fish. Carps eaten in spring were smaller than in summer. Moreover, in summer viperine snakes selected smaller carp than the available mean size. This divergent tendency between carp size selection and carp size availability reveals how seasonal diet shifts in prey size selection may be a response to an increase in prey size.

The seasonal decline in egg-volume on the basis of the individual changes in 35 known-age female Common Terns Sterna hirundo was studied with data available for 3-6 years. Interindividual differences were significant for both laying dates and egg-volume. Thus, to examine whether the decline was an effect of an assortment of females with the timing of breeding according to their individual egg-volume, the individual values of laying date were correlated with egg-volume. Correlation was not significant, suggesting that seasonal decline in egg-volume was not due to interindividual differences. Instead, egg-volume showed a gradual decrease with the progress of the season at intraindividual level i.e. the later an individual female laid in the season the smaller was the egg-volume. Moreover, the seasonal decline in egg-volume was not due to a simultaneous increase in egg-volume with an advancement of the timing of breeding with age, since the rate of seasonal decrease in egg-volume was similar for young and old birds. Individual average of laying date accounted for 22% of the individual average of fledgling production whereas egg-volume was not significantly correlated with fledging production. Thus, it is suggested that seasonal decline in egg-volume is a non-adaptive consequence of physiological effect of condition on both timing of breeding and egg-volume.

We investigated which of three hypotheses (better option, incompatibility or asynchronous arrival) best explains divorce in the common tern. One partner did not return the next year in 18.5% of 150 pairs. Among the 106 pairs in which both mates returned, the divorce rate was 18.9%. We found no significant differences in: breeding performance or condition in relation to the probability of divorce; quality of previous mates and new mates, mean age in relation to pair bond status; breeding success before and after divorce nor did this differ from breeding success of reunited pairs. Hence the better option and incompatibility hypotheses were not supported. However, divorce was more likely in pairs in which mates arrived asynchronously on the breeding grounds, supporting the asynchronous arrival hypothesis. Median arrival asynchrony for divorced pairs was 7.5 days and for reunited pairs 2 days; mates arriving more than 16 days apart always split up. About 20% of divorced birds lost breeding status in the year of divorce, probably as a consequence of their late arrival. Our results suggest that terns search for a new mate as soon as they arrive on the breeding grounds and that mates remain faithful to each other to avoid the costs of searching for a new partner. Thus, synchrony in arrival facilitates pair bond maintenance rather than asynchrony promoting divorce, since divorce appears to be a side-effect of asynchrony and not an active decision.

(20) Within and between season nest-site and mate fidelity in common terns Sterna hirundo. Journal für Ornithologie 140: 491-498.

González-Solís, J., H.Wendeln & P.H.Becker (1999)

We studied nest-site and mate fidelity in renesting Common Terns (Sterna hirundo), from 1993 to 1997, in a Common Tern colony breeding on six small artificial islands in the harbour area in Wilhelmshaven (German North Sea coast). Implanted transponders made possible individual recognition of the adults throughout their lifetime. We compared intra-season rates of nest-site and mate fidelity with between-year rates. Intra-season divorce was never observed. On the other hand, inter-year divorce was estimated at about 25%, suggesting that the costs of intra-season divorce are higher or opportunities for divorce are lower than between years. For 75% of 26 pairs that renested within the same season, the distance moved was less than 4.3 m. For 75% of 57 faithful pairs between two consecutive seasons, the distance moved was less than 1.25 m. Dispersal distances between and within years did not differ significantly but were clearly shorter than a paired random distribution of nests. Most of the birds changed the nest-site either when renesting or between years, but most of the new scrapes can be considered as lying within the original territory. Thus, failure of the first breeding attempt increased neither divorce nor nest-site dispersal as compared with between-year rates. We also studied the influence of parental age and the date of breeding failure on distances moved in renesting birds. Late renesting pairs did not change the nest-site. A multiple regression analysis revealed that the longer the duration of the first breeding attempt and the older the pair, the shorter the nest dispersal distance.

From 1993 to 1995 we analysed the influence of purse-seine and trawler fisheries on the activity cycle of Audouin's Larus audouinii and Yellow-legged Gull L.cachinnans at the Chafarinas Islands (Moroccan coast). Yellow-legged Gulls showed an activity cycle mainly diurnal. However, they take advantage of the fish attracted to the surface by purse-seine boats during the night and dawn. Purse-seine fisheries use powerful lamps that aid the gulls in localizing and exploiting schools of fish concentrated around lamps. Audouin's gull did not show any activity peak either during the day or at night. Although Audouin's Gulls showed conspicuous activity peaks depending on the trawler time-table in other colonies, the difficulty of predicting trawling fishery discards at Chafarinas Islands probably underlies the absence of clear peaks of activity linked to this fishery. Data suggest that parents foraged and relieved each other continuously. The capacity to maintain a continuous trophic activity, day and night, could be an additional advantage when the energetic costs increase during the reproductive period.

Migration costs are usually evaluated in terms of energy expenditure or risk of predation. Although parasitism has been widely recognized as an important potential factor for fitness, few studies have dealt with the relationships between migration and parasitism. From 1991 to 1997, birds in the surroundings of Santa Susanna village (Catalan Maditerranean coast) were regularly mist-netted and ringed. Blood smears from 13 common passerines species were collected during the reproductive period and examined to detect the presence of blood parasites. Migratory status of these species was assigned according to the analysis of captures and recaptures over the year. Some dipterous samples were also collected with a Malaise trap installed near the ringing station and determined to species level. Five bird species were classified as a partial or total migrants and 8 species were classified as a residents. Of the five migrant species, four presented at least one individual infected by haematozoan parasite. Only one species showed infected individuals among the eight species classified as residents. At least three dipterous species were identified in the study area as a potential vector for haematozoan haemoproteids. We suggest two ways to explain these results. 1.- The habitat-heterogeneity hypothesis: the degree of exposure of host species to parasite vectors has been reported as the main factor to explain parasite prevalences. In spite of the presence of some potential vectors in the study area, haematozoan parasite species seem to be absent from the area. Migrant species were parasitized outside of the study area, in the winter quarters or during the stop-overs in migration. 2.- The immunosuppressive hypothesis: immunosupressive effects of large energy investment of host species can increase the level of infection. Resident species are not so stressed as migrant species. Resident species, although infected, can mantain parasite infection below the detectability level or they can recover from infections. Migrant species are more likely to develop acute infections as a consequence of the migratory effort.

It is hypothesized that birds will retain the nest site after successful breeding performance but will move to another nest site after breeding failure. Similarly, improvement in the breeding performance has been suggested as a benefit of nest site fidelity. Although breeding success and nest site fidelity can also be influenced by age and duration of pair bond, few studies have analyzed the interactions of these variables with nest site fidelity. From 1992 to 1996, Common Terns Sterna hirundo of a colony at Wilhelmshaven fitted with transponders, were studied and their nest sites, mates and reproductive performace were checked each year. The nest distance between year n and year n+1 was measured for individually identified females. The potential causes of nest site fidelity were analyzed by relating fledging success in the year n, laying date in the year n+1, arrival date in the year n+1 and body condition in the year n+1 with nest site fidelity. The potential benefits were investigated by relating the breeding performance in the year n+1 (i.e. laying date, egg volume, clutch size, hatching success and fledging success) with nest site fidelity. When age and pair bond are not considered, it appears that the timing of breeding is the cause of nest site fidelity, and a greater success is the benefit. However, when age and pair bond status are included in the multiple regression analysis, it reveals that these variables are more likely to determine nest site fidelity than the mentioned above. Older and faithful birds showed a higher nest site fidelity than younger divorced or widowed birds. Mechanisms which could influence the ability of older and faithful birds to occupy the same nest site year after year are discussed.

A significant decrease in the body size of Audouin's Gulls Larus audouinii breeding at the Chafarinas Islands is reported. The decrease in linear measurements in the current breeding population ranged from 2.5% to 5.6% in males and from 0.61% to 4.4% in females. This was detected when assessing the reliability of a sex-discriminating function derived for the same colony 13 years earlier. When applied to the current population, this function failed to predict the sex of a large proportion of males (44%). The relative decrease in mean size was significantly greater in males than in females for culmen, nalospi and tarsus lengths, while for bill depth at culmen, wing length and body mass the relative decrease was similar in both sexes. Since the extent of differences depended on sex, these differences cannot be attributed to a systematic between-observer bias. Mean body size reduction might be either the result of a greater proportion of small breeding birds in the current population, because of increased availability of nesting sites (competition relaxation hypothesis), or an outcome of environmental factors affecting growth parameters (environmental constraint hypothesis). According to the first hypothesis, the changes observed would be associated with higher variability values. Conversely, if the second is true, the degree of variability should be similar. Since there are no significant differences in the degree of variability shown in the two data sets, our results support the second hypothesis. The environmental constraint acting via growth parameters is probably related to the increase in the number of Audouin's Gull breeding pairs while food availability was depleted. Our data suggest that changes in the duration of the growth period, rather than in the growth rates themselves, are involved in the body size differences found.

The diets of two potential competitor species, Audouin's Larus audouinii and yellow-legged gulls Larus cachinnans, were examined while they bred at the Chafarinas Islands during 1993, 1994, and 1995. Data were collected during two commercial fishing regimes: (1) trawling and purse seine fisheries, and (2) diurnal trawlers only. Since the food supply for the gulls in this area was heavily reliant on the activity of purse seine fisheries, these contrasting situations allowed us to analyze short-term effects, induced by daily changes in food supply, on niche width, dietary shift, and niche overlap between the two species. Overall, both species relied mainly on fish for food, especially Clupeiforms, in the case of Audouin's gull irrespective of the fishing situation, and in the case of the yellow-legged gull, only when purse seine fishing was in operation. When purse seine boats did not operate (food shortage), yellow-legged gulls broadened their niche, consuming equal amounts of all the feeding resources, and they showed a dietary shift toward a greater consumption of prey from refuse tips. In contrast, Audouin's gulls did not change their niche width, but showed a slight dietary shift away from the consumption of epipelagic fish, compensated by an increase in reliance on benthic-mesopelagic resources. Niche overlap was clearly higher on days when both fishing fleets operated, probably because a superabundant food resource facilitates high overlap without affecting coexistence between the two species. Since our study was developed on the basis of daily variations in food supply, and competition effects are to be expected on a longer-term basis, these changes can be seen as the outcome of the coexistence of two species in stable competitive equilibrium.

We examined blood smears of 38 adult Corys Shearwaters Calonectris diomedea (O. Procellariformes) from Chafarinas Is. (Moroccan Mediterranean coast). Although the presence of the haemoproteid vector at this locality (Ruiz et al. 1995), no Shearwater was infected. Pelagic habits of most Procellariiformes are an unsuitable environment for potential vectors of haemoproteids.

We analyzed five different types of food samples from Audouin's Gull (Larus audouinii), collected during the breeding seasons of 1994 and 1995 at its two main breeding colonies, the Ebro Delta and the Chafarinas Islands. These food samples included spontaneous regurgitates, dry boli containing partially digested food, food remains, pellets, and prey identified during direct observations of chick provisioning. We compared estimates of biomass, levels of taxonomic determination allowed by each kind of food sample, and the associated potential biases to assess which sampling method provides the best estimate of diet in gulls. Regurgitates allowed identification of most prey to species level and reliable biomass estimates, but their collection was time-consuming and invasive. Dry boli provided almost the same information as regurgitates at order level and were easy to collect. However, both underestimated soft-bodied prey and prey with large, hard parts. Food remains provided an estimate of diet composition that was highly biased towards prey with large distinctive hard parts. However, food remains were a good complement to dry boli, enhancing biomass estimates for food items that had a good relationship of weight and linear measurements of prey hard parts. Direct observation allowed identification of prey only to upper taxonomic categories, and is useful when only a broad categorization of prey types is required. Pellets showed important biases towards fish with robust otoliths, and inaccurate conversion to biomass, but they can be useful for monitoring variations in the consumption of certain prey items. Several factors such as time spent collecting, sample availability, disturbance to animals, and the status of the species studied need to be considered when deciding on a method of diet assessment sampling.

(12) Influence of food availability on interactions between Larus cachinnans and L.audouinii. Canadian Journal of Zoology 75: 719 724.

González-Solís, J., X.Ruiz & L.Jover (1997)

Predatory and (or) kleptoparasitic interactions are greatly facilitated in mixed-species gulleries, particularly when one of the species is larger than the others. Larus audouinii, the Audouin's gull, is a threatened species breeding in sympatry with the larger L. cachinnans, the yellow-legged gull, throughout the Mediterranean. The yellow-legged gull has often been cited as the main threat to the Audouin's gull. On the Chafarinas Islands, the second largest breeding place for Audouin's gulls in the world, both gull species depend largely on commercial fisheries for food. We analyze the influence of food availability, assessed through fishery activity, on the frequency and intensity of interaction pressure by yellow-legged gulls upon Audouin's gulls during the breeding season. We studied five different types of interaction: (1) flyovers of yellow-legged gulls; (2) ground intrusions; (3) egg predation; (4) chick predation, and (5) aerial kleptoparasitism. Moreover, intensity of interaction pressure was assessed using logistic regression analysis to build a model of the relationship between Audouin's gulls' response to yellow-legged gull flyovers as a dependent variable and fishing fleet activity as an independent variable. All interactions except aerial kleptoparasitism were significantly more frequent during days without sardine fishery activity. However, chick predation is significantly higher only during the period when yellow-legged gull has fledglings. Overall, the results of the estimated logistic model indicate a positive association between depleted food and the response by Audouin's gulls to aerial intrusions.

The diet and feeding ecology of breeding Audouin's Gull Larus audouinii were examined at the Ebro Delta (NW Mediterranean) during 1993, 1994 and 1995 and found to depend on the activity of the commercial fisheries operating within the foraging range of the birds in the colony. One of the largest fishing fleets in the Mediterranean operates in this area, both diurnal inshore trawlers and nocturnal purse-seine boats. Since 1991, a trawler moratorium has coincided with the breeding season of the gull, which has affected its feeding ecology in the area. Data were collected under four commercial fishing regimes: diurnal trawling only, diurnal trawling and purse-seine fishing at night, night fishing only, and no fishing. Although Audouin's Gull usually feeds mainly on epipelagic fish (65% by biomass on average), in our study they depended largely on trawler discards (benthonic fish represented up to 73% by biomass when only trawlers operated) because they are a food source with a high energetic value and are predictable in space and time. The active capture of clupeiform fish significantly increased when trawlers were not operating, and the gull has also broadened its foraging niche to feed in rice fields, in ecotonic habitats and occasionally on refuse tips, suggesting that the clupeiform population was not large enough to meet the food demands of the colony. The presence of some discard prey in the diet when trawlers were not operating indicated that some breeding gulls were able to travel beyond the area affected by the moratorium (more than 110 km from the colony). The frequency of incubation changeovers did not change significantly when trawlers were not operating, but marked changes brought about by the moratorium were recorded during the chick-rearing stage. Chick feeding frequency significantly decreased during the trawler moratorium, although the number of prey per regurgitate delivered to chicks did not vary. When trawlers did not operate, adults seemed to compensate for the lower food availability by carrying larger prey items to the chicks. In contrast, chicks occasionally did not accept the regurgitated food, especially when trawler discards were available. The trawler moratorium affected not only the diet of Audouin's Gull but also the adult time budgets and the provisioning rates of chicks, although the species showed some ability to buffer against low food availability.

(7) Succession and secondary production of gastropods in the Ebro delta ricefields. Hydrobiologia 337: 85 92.

González-Solís, J. & X.Ruiz (1996)

We analyze the dynamics of the gastropod community living in the Ebro Delta ricefields in relation to the cultivation cycle. We found six bassommatophoran species, three of which form more than 97% of total: Physella acuta (Drapamaud), Planorbis planorbis (L.), and Lymnaea ovata (L.). Colonization of ricefields by freshwater pulmonates started in June, with populations increasing until the harvest in October, when the bulk of gastropods showed a decline. Populations recovered quickly and, in some cases reached their yearly maximum of abundance in Autumn. P. acuta predominates over all other species except in May and June, when P. planorbis takes over. L. ovata is always the least abundant of the three main species. The analysis of size classes suggests that there are two breeding seasons for P. acuta and P. planorbis, the first at the end of Summer, with a total replacement of the population, and the second in October-November, without replacement. Conversely, L. ovata shows a wide array of size classes throughout the rice cultivation cycle. This suggests that this species breeds earlier and outside the ricefields. The variations in population numbers of each species in relation to environmental changes induced by rice cultivation suggest that P. planorbis better exploits the algal cover or other food available at the ricefield bottoms, while P. acuta forages mainly on epiphytic vegetation and adventitious plants. Gastropod secondary production and turnover ratios in the Ebro Delta fall within the range of natural freshwater systems, but are situated in the zone of higher values.

Differences in diet between chicks and adults of Audouin's Gull (Larus audouinii) were examined in the Chafarinas Islands (SW Mediterranean) during the breeding seasons of 1993 and 1994. The major food type for chicks on the Chafarinas Islands were fish, both epipelagic (some of them coming from purse-seine activity) and benthonic (coming from trawler discards), forming a minimum 70% by biomass. The amount of fish fed to young increased with chick age, up to 95% by biomass. Adults also consumed mainly fish (75% by number and 90% by biomass), mostly clupeiforms, Sardina Sardina pilchardus and Gilt Sardine Sardinella aurita. Terrestrial prey were especially given to youngest chicks, probably because of requirements to increase the feeding rates of these chicks. Shorter foraging trips to the near Moroccan coast to feed on terrestrial prey (chiefly arthropods and isopods) may allow adults especially to guard/brood small chicks, and eventually to increase the feeding rates. Feeding rates significantly decreased with brood size, and also decreased with chick age, although this effect was not significant. Weight and size of food items fed to chicks increased with chick age, and fledglings were fed with larger items than those consumed by adults. This was probably related to the increase in gape width with chick age, and the trade-off between food load carried to feed chicks and the distance traveled to catch the fish.

The Ebro Delta ricefields are used by waterbirds mainly as foraging
habitats, since other uses, such as nesting, are restricted to a few
species. The availability of freshwater wetlands in the Ebro Delta depends
chiefly on the hydrological cycle derived from rice culture. We present data
on seasonal variation in availability of potential waterbird prey items
(macroinvertebrates and vertebrates) in the Ebro Delta ricefield system, and
discuss their relevance for waterbird populations living there during an
annual cycle. Biomass/length predictive equations are presented for those
prey items more relevant to waterbird populations

Data on haematozoan parasitemias in Laridae are scarce and, since blood parasites may influence birds fitness, it is important to report their incidence in threatened species such as Audouin's Gull. Blood smears of 90 Audouin's Gulls caught during incubation at their two main breeding sites, the Ebro Delta and the Chafarinas Islands, were examined for Haemoproteus lari parasitemia. Overall prevalence of the parasite was 92.1% for the Chafarinas Is. and 28.9% for the Ebro Delta. To our knowledge, prevalences reported in this study are the highest ever recorded for Laridae. Differences in prevalence between localities were significant, but not differences between the sexes within each locality. Males were more intensely parasitized than females in Chafarinas, and gulls from Chafarinas were more intensely parasitized than those from the Ebro Delta. Differences in intensity were also checked using data from sympatric Yellow-legged Gulls, L. cachinnans, and only the factor locality was significant. There was no significant relationship between body condition and intensity of parasitemia in males or females in either locality. These data indicate higher susceptibility to H. lari for gulls breeding at the Chafarinas Islands.

This paper deals with two factors affecting density and distribution of nests of Yellowlegged
Gull in the Medes Islands colony, both the habitat and the culling. Nest density was
significantly lower on coastal cliffs than on the other parts of the islands. Vegetated areas,
when suitable, allowed higher nest density. Clutch size was not different among the study
areas. The colony size does not seern to have decreased since the last census in 1991 (Fortia
and Hontangas 1991). However, we recorded a significant decrease in the nest density in Borne
study areas.

The gastric contents of 92 Song Thrushes caught in Majorca (n = 47) and Montsia (n = 45) during October 1986 have been analyzed. Diet composition is given for taxonomice and typological prey-types. Song Thrushes of Majorca consumed high quality resources homogeneously, whereas those of Montsia used suboptimal resources heterogeneously. Trofic differences found between ecomorphotypes need further studies to be adequately interpreted. The energy conversion of prey types clearly show that olives are the most song thrushes\' profitable prey. The need of animal prey consumption related to protein deficit and the alternative coevolutive hypothesis are discussed. Estimations of the costs of Song Thrush feeding activity during the winter to olive harvest are given.

Ngone Diop

"Population ecology and movements of Audouin's gulls and tropicbirds in Western Africa". University of Dakar, Senegal. Supervised by Professor Cheikh Tidiane Ba (Dakar University) and Jacob González-Solís