The network depicts up- and down-regulated TFs (green and red, respectively) along with their miRNA targets (blue and pink). The module was set to identify TFs functioning as activators and therefore targets and regulators tend to be correspondingly differentially expressed. Each regulator node (green/red nodes) hosts the regulator name, while target nodes (blue/pink nodes) depict the number of grouped targets. By selecting a target node all grouped targets are presented in the relevant pane (bottom right).

Availability – DIANA-mirExTra v2.0 has an intuitive user interface and is freely available to all users without any login requirement. http://www.microrna.gr/mirextrav2

Categories

Categories

Archives

Archives

What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)