Thank you for having your post-doc provide the protocol and image. Is she confident that the samples are not degraded? The smear around the 49kDa marker suggest that might be the case, but I do not have an explanation for absence of signal for the reco...

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results. We are not aware of any problems with this antibody. We have also not received any negative feed...