Flow Cytometry Analysis of Apoptotic Markers

Analyze the effect of your compound on early and late stage apoptotic cell populations

Horizon offers FACS for the identification of early and late apoptotic cell populations using FITC-Annexin V (apoptosis) and propidium iodide (PI, viability). Annexin V binds to membrane phospholipid phosphatidylserine (PS) that in apoptotic cells is translocated from the inner to the outer leaflet of the plasma membrane exposing PS to the extracellular space. Viable cells with an intact plasma membrane exclude PI, whereas the plasma membranes of dead and damaged cells are permeable to PI.

Viable cells are Annexin V and PI negative

Early apoptotic cells are Annexin V positive and PI negative

Late apoptotic cells (or the cells that are already dead) are Annexin V and PI positive