Abstract: Production of pullulanase was carried out from I. batatas, P. vulgaris, E. crassipes and P. stratiotes using A. pullulans. The fermentation reactions were carried out in minimal basal media at 28°C. E. crassipes and P. stratiotes as substrates produced more pullulanase than I. batatas and P. vulgaris. The highest enzyme production from Pistia stratiotes was of 9.3 U/ml after 72 hours of incubation at 28°C and Eichhornia crassipes showed most enzyme production of 4.65 U/ml after 48 hours of incubation at 28°C. The two novel substrates, E. crassipes and P. stratiotes, could successfully be used for the production of pullulanase and are a promising resource for large-scale production of the same.

[24]. Mishra, B., Vuppu, S., "A Study on Downstream Processing for the Production of Pullulan by Aureobasidium Pullulans-SB-01 from the Fermentation Broth." Research Journal of Recent Sciences 2, pp. 16-19, 2013.