Zetalif LED

01 Overview

The Zetalif LED induced fluorescence (LEDIF) detectors are sensitive solutions for all separation techniques such as Liquid Chromatography (capillary LC, microLC, nanoLC, UPLC) and Capillary Electrophoresis. A large range of LED wavelengths is available: 365, 450, 480, 530 and 640 nm.
Picometrics developed this LED based detector to propose the analysts almost equal sensitivity as laser technology with a significantly reduced cost.

02 Technology

The key feature of this patented technology is the use of a ball lens in direct contact with a capillary or micro- column, enabling both high fluorescence recovery and ease-of -use. Fluorescence is read directly inside the capillary, without degrading the resolution of the analytical chain. No optical fine tuning is required when changing the capillary.

Moreover, recent upgrade of this unique technology allowed a superb miniaturizationof the optical head, linked to the light source and detector by optical fibers. This design makes easy the integration of the detector in any analytical system.

03 About LEDs

Compared to lasers, wavelength width is wider and the light is not coherent. These features make Zetalif LED detectors a bit less sensitive than Zetalif Laser detectors. The loss of sensitivity will depend on the exact application, it is a two to five-fold decrease in most cases.

LED also offers practical advantages:

The LED is completely integrated inside the detector casing

Lifetime is very long (>20000 hours) and cost is reduced compared to lasers

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Picometrics Zetalif Laser induced fluorescence (LIF) detectors are ultra sensitive solutions for all separation techniques such as Liquid Chromatography (capillary LC, microLC, nanoLC, UPLC) and Capillary Electrophoresis.
The modular design of this detector provides maximum versatility and enables an unrivalled level of sensitivity for fluorescence measurement thanks to a broad range of lasers offering an excitation wavelength from 266nm to 785nm. Analysts can adapt the wavelength of the laser depending on their application, simply by changing the laser and the corresponding filter blocks.

The Zetalif LED induced fluorescence (LEDIF) detectors are sensitive solutions for all separation techniques such as Liquid Chromatography (capillary LC, microLC, nanoLC, UPLC) and Capillary Electrophoresis. A large range of LED wavelengths is available: 365, 450, 480, 530 and 640 nm.
Picometrics developed this LED based detector to propose the analysts almost equal sensitivity as laser technology with a significantly reduced cost.