It’s The Wolf

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Synopsis: It’s the Wolf!, a segment of Cattanooga Cats, followed the comic exploits of a wolf named Mildew (voiced by an uncredited yet easily recognizable Paul Lynde), who aspires to catch and eat a sure-footed little lamb named Lambsy (voiced by Daws Butler), but is always thwarted in this plan by the dog Bristle Hound (voiced by Allan Melvin). Bristle would apprehend Mildew (usually after hearing Lambsy’s cries of, “It’s the wool-uff!”), pound him, and toss him sailing into the air, with Mildew screaming a rejoinder such as “Spoil Sport!” as he flies into the horizon and lands with a thud. Showing modest responsibility, Lambsy never fails to recognize Mildew.
Source: Wikia