NovAliX’ SPR instruments are highly sensitive. Both commercial and proprietary systems are available with complementary advantages in throughput, format, workflow and information delivered. The high level features of these formats are as follows:

Commercial channel-based Biacore™ systems

Medium throughput ligand screening

Hit confirmation

Characterization of compound/protein binding

Detailed analysis protein/ligand Interactions

Proprietary NovAliX chemical microarray SPR

Higher throughput primary screening

Fragment based drug discovery

Affinity ligands for bio-separation (downstream processing)

A technology platform for molecular recognition

In each format, one of the potential binding partners is immobilized on a sensor surface whist the other is in solution. Binding of the soluble analyte to the immobilized partner induces a change in refractive index close to the surface that can be read by SPR. The sensor surfaces are gold coated which permits functionalization in conventional systems this enables the coupling of the protein of interest to the sensor while in the microarray format thousands of small molecules are immobilized on a single chip.

SPR assays support many applications in research, development and manufacturing. NovAliX has considerable experience in the use of SPR for drug discovery and bio-separation. Measurements include binding affinity, binding kinetics and selectivity. Modern instruments are even sensitive enough to detect weak binding of low molecular weight analytes, a prerequisite for fragment based drug discovery.

NovAliX uses commercial channel-based GE BiacoreTM instruments but the array-based systems are exclusively designed & produced for in-house use. This combination creates more opportunities to address challenging issues.