Senator Burston said on Wednesday he was the man referred to, telling News Corp Australia it was "bull***" and one of the reasons he left One Nation was because of sexual harassment from Senator Hanson over two decades.

He claimed Senator Hanson "rubbed her fingers up my spine" while listening to the national anthem and she'd propositioned him after he was elected in 2016 at her home in Queensland and Canberra.

But Senator Hanson denied all claims.

"I wouldn't go near him with a barge pole," she said.

Parliament scuffle

The tensions erupted in Parliament House on Wednesday evening when Senator Burston got involved in a scuffle with One Nation advisor James Ashby.

The Australian newspaper published images showing the pair in a physical clash on Wednesday night, after Senator Burston had earlier accused One Nation leader Pauline Hanson of sexually harassing him.

Photos of Brian Burston’s injured hand - he alleges James Ashby caused it. Ashby says footage and witnesses will better explain what happened. Our story on all the tension today leading up to tonight’s fight: https://t.co/KiWpgfZIfDpic.twitter.com/2owetLMy3R