The tasmanian tiger, Thylacinus cynocephalus, seen here at the Washington DC zoo in 1902, has been extinct since 1936. (Source: Wikipedia)

The mouse embryo, seen here, is now part thylacine -- it contains thylacine DNA which will effect its muscle and cartilage growth. (Source: Andrew Pask and Richard Behringer)

Russian researcher Sergei Zimov is building a vast 160 sq km "Pleistocene Park" in northeastern Siberia near Alaska. He hopes to one day populate it with wooly mammoths and sabertooth tigers. (Source: BBC News)

By injecting DNA sequenced from an extinct species into a mouse embryo, scientist have delivered a proof-of-concept, which could one day lead to full cloning of long-extinct species

Cloning extinct species has been a long standing dream of mankind. Well, long standing since the 90s at least, when the movie Jurassic Park stole the imagination of audiences worldwide with a fictitious story in which scientists use DNA extracted from long dead dinosaurs to resurrect the beast through cloning (with disastrous results). Since the movie, cloning science has advanced at a steady pace, and there has been increasing interest within the scientific community in cloning extinct creatures.

While it seems unlikely that dinosaur DNA pristine enough to produce a full genome map would ever be found, it would be reasonably easy to sequence other extinct creatures genomes such as the dodo, Neanderthals, wooly mammoths, and other more recent critters which researchers have recovered soft tissue samples (bone marrow, skin, etc) from. However, such dreams were often scoffed at and remained the realm of pseudoscience.

They were derided -- until now. In an exciting, successful new experiment, researchers at the University of Melbourne report that they have taken DNA from the extinct thylacine, commonly known as the Tasmanian tiger, and inserted it into a mouse embryo which continued to grow with the thylacine gene in place. The process represented the first time DNA from an extinct creature was inserted into an embryo, effectively bringing "back to life" in part the extinct species.

Dr Andrew Pask, who led the research, says the experiment was the first time DNA from an extinct species has been successfully used "to induce a functional response in another living organism". Professor Marilyn Renfree, a member of the team, states, "For those species that have already become extinct, our method shows that access to their genetic biodiversity may not be completely lost."

The findings of the team will be reported in the international science journal PLoS ONE this week.

The Tasmanian tiger, whose scientific name is Thylacinus cynocephalus or thylacine for short, is an extinct marsupial from Australia that looked like a wolf. The last surviving member of the species died in captivity at the Hobart Zoo in 1936. The gene was extracted from tissue samples of flesh preserved in ethanol, which were donated by the Museum Victoria in Melbourne Australia. The DNA was verified to be thylacine, and then was injected into the developing mouse embryo.

The gene injected was the thylacine Col2a1 gene, which controls cartilage and bone growth. The mouse embryo was selected, partially because mice have a similar gene, also labeled Col2a1.

Dean of Science at the University of New South Wales, Mike Archer, is leading a project that aims to fully clone extinct animals, but he warns that success remains far off. He commented on the developments, stating, "The next question then is, well what if you did that with the whole of the DNA of the thylacine? Could you in fact bring back a thylacine? Technically I think this is pretty difficult at the moment but on the other hand this is one very significant step in that direction and I'm delighted."

Despite the steep challenge, Professor Archer isn't giving up. He states, "I'm personally convinced this is going to happen. We are working on a number of projects like this. I've got another group working on another extinct Australian animal and we think this is highly probable."

Aside from possibly leading to clones of extinct animals, the breakthrough also may yield scientific gains in biology and medicine. Professor Richard Behringer, Deputy Head of the Department of Molecular Genetics, M.D. Anderson Cancer Center, at the University of Texas, says, "This research has enormous potential for many applications including the development of new biomedicines and gaining a better understanding of the biology of extinct animals."

Perhaps most ambitious is the Pleistocene Park currently being built in northeast Siberia by Sergei Zimov. Zimov introduced a grassland ecosystem along with 100 large mammals includingreindeer, horses and moose. He plans on eventually introducing to the park wooly mammoths, extinct saber tooth tigers and prehistoric bears; all cloned of course. On the possible threat of cloned tigers he states, "OK, so one or two people will be killed, like in India, but far more will die of alcohol in this place than from tigers."

While Zimov and others have thus far not revived any extinct species, advances in cloning may one day make their dreams a reality. And with the new Australian breakthrough, that day seems to have come much closer.

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How could an extinct species embryo be cloned without a donor egg from the same species of animal? Then, how could the cloned embryo of an extinct species be brought to term without a carrier of the same species?

Sometimes I wonder if most people read more than the headline before they post here. It says right in the article that all that is needed is soft tissue of some kind (skin,marrow etc..). It is also not a cloned embryo, they merely changed 1 gene in the DNA. I assume they can use a mouse because the gene is question (Col2a1) is so very close to the gene that is found in the mouse(Col2a1).

Yes, I had searched on wikipedia for the idea of using different species as carriers and I could not find anything.

quote: physically similar animal

So it sounds like an ape could give birth to a human or a woman could give birth to an ape? That's very freaky. If you're correct, I suppose there is no chicken-and-egg problem wrt cloning extinct animals like I thought there would be.

Sometimes I wonder if most people read more than the headline before they post here. It says right in the article that all that is needed is soft tissue of some kind (skin,marrow etc..). It is also not a cloned embryo, they merely changed 1 gene in the DNA. I assume they can use a mouse because the gene is question (Col2a1) is so very close to the gene that is found in the mouse.

Really. That's very interesting. I just assumed that an elephant would reject (abort) a woolly mammoth embryo or a canine bitch would do the same to a thylacine embryo. If what you say is true (it's very hard to find research on this topic) then it would seem that there is very little holding back the science community from just resurrecting any extinct animal they have the complete DNA of.

More importantly, how do you explain to said mouse/dino why he doesn't look like the other mice? How do you explain to a 5 year old that his dad loved him, but has been extinct for a million years. Oh that's going to be one messed up mouse with some bad daddy complexes.