Recombinant protein was produced with TrueORF clone, RC200003. Click on the TrueORF clone link to view cDNA and protein sequences.

Tag:

C-terminal MYC/DDK

Predicted MW:

43.5 kDa

Purity:

> 80% as determined by SDS-PAGE and Coomassie blue staining

Mass Spec Validation:

This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology.

Concentration:

>50 ug/mL as determined by microplate BCA method

Buffer:

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Preparation:

Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

BioActivity:

TP53 Activity Verified in a DNA-binding Assay: TP53 (TP304649) activity was measured in a colorimetric DNA-binding assay. Double-stranded oligonucleotide containing the p53 consensus DNA-binding sequence was incubated with dilutions of the purified TP53 protein and TP53 bound to the oligo was captured onto the surface of a microtiter plate. After washing, bound TP53 was detected with an anti-p53 primary antibody followed by an HRP-labeled secondary antibody. After initial color development, the reaction was quenched and the color intensity was measured at 450nm.

Summary: This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013].

*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information

**: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich