CRMB :::: Vol.4, No.2, March - April 2016

Current Research in Microbiology and Biotechnology is an international open access bi-monthly journal that covers all aspects of latest research in microbiology and biotechnology. Manuscripts are published soon after peer-review.

Leila Goudarzi et al. Current Research in Microbiology and Biotechnology 4(2) 2016: 827-834

827-834

ABSTRACT:Lactobacillus species are Gram-positive, non-spore forming, spherical or rod-shaped and catalase-negative bacteria which have beneficial effects for their host. This study aimed to investigate the effect of Lactobacillus species against some Proteus species virulence factors. In this study after determining MIC and sub-MIC for Lactobacillus strains culture supernatants, growth, urease activity and swarming migrations of Proteus species were assessed. The results showed that the cultural supernatant of Lactobacillus strains in all cases, had inhibition zone between 11 to 20 mm and also could eliminate or inhibit urease activity and swarming migration (p-value<0/05). According to the results of this study, lactobacilli have inhibitory effect on growth, swarming movement and urease activity of Proteus species as a major virulence factor of these bacteria. So it is hoped that Lactobacillus species as a probiotic bacteria can be used to treat infections caused by Proteus bacteria, particularly antibiotic resistance species.

Consuelo Nieto-López et al. Current Research in Microbiology and Biotechnology 4(2) 2016: 835-841

835-841

ABSTRACT:A surface response design methodology was used to optimize laccase production by Pleurotus djamor. Among six strains ECS-0184 was selected to apply the mathematical approach. ECS-0184 showed a specific growth rate (u) of 0.87 days-1 and a laccase yield (YL/X) of 372 UA g-1 biomass. Predictable optimal conditions obtained were pH 5–6, temperature range 25–28 C and 3–5 days of cultivation to obtain best laccase yield, 1175 UA g-1 biomass. Critical factors for laccase production are temperature and day of harvest according with experimental design. The laccase produced by Pleurotus djamor has a molecular weight of 44 kDa; its activity was detected in PAGE gels with ABTS and pyrogallol as substrates.