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In vitro Root Induction of Camellia sinensis L.

Title In vitro Root Induction of Camellia sinensis L.

Author Mrs. Jindanee Yompakdee

Degree Master of Science (Biotechnology)

ABSTRACT

Shoots of Camellta sinensis L. (1.5-2.5 cm in length) derived from sterile plantlets were cultured on MS medium containing BA at 0, 1, 2, 3, 4 mg/l combined with IBA at 2, 4, 6, 8 mg/l and activated charcoal (6 g/l). Before culturing, The shoots were dipped in a solution containing 500 mg/l IBA for 1 min. The shoots were cultured for a period of 3 months with the first 2 weeks in darkness and then 10 weeks in light conditions.

The result showed root formation occurred in that most of the medium combinations BA and IBA at the ratio of 0:2, 0:6 and 2:8 mg/1 yielded 100% root induction. However, the number and length of roots varied among the three media. The medium containing BA and IBA at the ratio of 0:2, 0:6 and 2:8 mg/1 gave the average root number at 13.0, 16.6 and 15.5 roots/shoot and the average length of roots at 1.9, 1.2 and 1.8 cm respectively.