The authors report that low percentages of dimethylsulfoxide ( DMSO ) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom up proteomics by up to tenfold. The method can be easily implemented on any LC-MS/MS system without modification to hardware or software and at no additional cost.

6.84E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

4.6E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

3.5E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

3.31E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

2.66E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

2.33E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

2.28E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

2.1E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

1.83E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

1.73E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

1.66E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

1.59E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

1.58E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

1.53E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

1.49E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

1.46E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

1.4E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

1.38E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

1.23E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

1.15E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

1.11E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

1.11E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

1.03E2Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

9.59E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

9.38E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

9.03E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

9.02E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

8.4E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

8.34E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

8.29E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

8.21E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

7.67E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

7.65E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

7.59E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

7.51E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

7.23E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

6.86E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

6.81E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

6.7E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

6.68E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

6.45E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

6.26E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

6.18E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

5.98E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

5.78E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

5.28E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

4.68E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

4.67E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

4.62E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.

4.6E1Parts per MillionThe number of spectral counts (SC) is approximately proportional to the product of the protein concentration and molecular weight (MW), therefore we estimate the fraction of total molecules in parts per million (PPM) by $$PPM_i={SC_i\/MW_i}/∑↙{j=1}↖n {SC_j\/MW_j}$$ where MWi is the molecular weight and SCi is the number of spectral counts for protein i and the summation in the denominator is over all the proteins identified in the experiment for a given tissue and condition.