กระบวนการปฏิบัติเพื่อพัฒนาการจัดการโรคข้าวโพดโดยชีววิธีDevelopment produced of biological control for disease management of corn

Corporate authors (Thai):

Kasetsart University, Bangkok (Thailand)

Citation:

Proceedings of the 4th Workshop of Corn and Sorghum Research Project of Kasetsart University: Corn and sorghum yield increasing to improve the quality of life and environmental sustainability, Bangkok (Thailand), p. 243-259

Antagonist was applied as either seed or foliar treatments and the efficacy of disease suppression was determined by disease incidence and systemic resistance induction. Under greenhouse, the threshold concentration of each antagonist required for significant (P=0.05) suppression of BLS was 1*10**(6) and 1*10**(8) cfu/ml for seed and foliar spray applications respectively. Combined seed and foliar treatments with antagonist provided higher induction of plant resistance than application either seed or foliar alone, as account by correlated accumulation of defense-related enzyme activity, beta-1,3-glucanase. Field experiment that revealed 54.7 percent severe epidemic of BLS resulted in 62.2 percent disease control respectively according to the experimental model investigated. The procedure threshold of antagonist application obtained in this study including seed bacterization, 3-foliar spray intervals, adjusted to the production cycle of plant when reached 14, 21, and 28 days after planting significantly (P=0.05) provided highest disease suppression (62.2 percent disease control) and increased beta-1, 3-glucanase activity compared to seed bacterization with 2-foliar applications. Increasing frequency of antagonist spray to level higher than 3 times did not result in a significant improvement of disease suppression. The native biocontrol agent alternative used as either seed or foliar spray showed that strain PCL21 gave the best plant growth promotion of corn seedlings and seed germination where P38 demonstrated the higher disease suppression. Combined native strains of PCL21 seed bacterization followed by P38 foliar spray therefore, resulted in highest disease suppression, induction of resistance and yield which their control efficacy was equivalent (P=0.05) to a standard strain SP007s (ISR-P) and recommended chemical control.

Availability :

National Corn and Sorghum Research Center, Nakhon Ratchasima (Thailand)