Effect of depletion of the Nup107-160 nucleoporin on spindles assembled in vitro with sperm chromatin and in Xenopus oocyte extracts. Spindles are labeled with tubulin (red) and chromosomes are stained with DAPI (blue). Lower far right panel shows a rare conformation. See Orjalo et al 2006. Mol Biol Cell 17:3806-3808.