No significant cross-reactivity or interference between this analyte and analogues is observed.

Samples

Serum, Plasma and Tissue Homogenate

Assay Type

Sandwich

Detection Range

0.25 ng/ml - 8 ng/ml.

Sensitivity

0.1 ng/ml.

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Preparation and Storage

Store all reagents at 2-8 degree C

Product Note

Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Other Notes

Small volumes of NAT8L elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NAT8L purchase

MBS085684 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the N-Acetyltransferase 8 Like Protein (NAT8L) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.25 ng/ml - 8 ng/ml in biological research samples containing NAT8L, with an estimated sensitivity of 0.1 ng/ml. The ELISA analytical biochemical technique of the MBS085684 kit is based on NAT8L antibody-NAT8L antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NAT8L antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NAT8L. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma and Tissue Homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of NAT8L (hereafter termed this analyte) in undiluted original Human serum, plasma and tissue homogenate samples.

Camello-like protein 3; N-acetyltransferase 8-like protein

This gene encodes a single-pass membrane protein, which contains a conserved sequence of the GCN5 or NAT superfamily of N-acetyltransferases and is a member of the N-acyltransferase (NAT) superfamily. This protein is a neuron-specific protein and is the N-acetylaspartate (NAA) biosynthetic enzyme, catalyzing the NAA synthesis from L-aspartate and acetyl-CoA. NAA is a major storage and transport form of acetyl coenzyme A specific to the nervous system. The gene mutation results in primary NAA deficiency (hypoacetylaspartia). [provided by RefSeq, Dec 2010]

UniProt Comments for NAT8L

NAT8L: Plays a role in the regulation of lipogenesis by producing N-acetylaspartate acid (NAA), a brain-specific metabolite. NAA occurs in high concentration in brain and its hydrolysis plays a significant part in the maintenance of intact white matter. Promotes dopamine uptake by regulating TNF-alpha expression. Attenuates methamphetamine-induced inhibition of dopamine uptake. Defects in NAT8L are the cause of N-acetylaspartate deficiency (NACED). A metabolic disorder resulting in truncal ataxia, marked developmental delay, seizures, and secondary microcephaly. Belongs to the camello family.

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Disclaimer

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