This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.

The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.

Kit Specifications - Spin Column

Column Binding Capacity

10 μg

Size of DNA Purified

100 - 15,000 bp

Average Recovery

> 90%

Average Primer Removal

> 90%

Minimum Elution Volume

30 μL

Time to Complete 10 Purifications

15 minutes

Kit Specifications - 96-well

Binding Capacity Per Well

15 μg

Maximum Loading Volume Per Well

500 μL

Size of DNA Purified

100 - 15,000 bp

Average DNA Recovery

> 90%

Average Primer Reoval

> 90%

Minimum Elution Volume

50 μL

Time to Complete 96 Purifications

20 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.