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Miltenyi Biotec distribution:

As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is
committed to providing our customers around the world with the highest quality products.
In addition to direct selling in more than 20 countries in North America, Europe and
Asia/Pacific, Miltenyi Biotec also provides support for our customers through an
extensive distributor network covering dozens of additional countries.

As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is
committed to providing our customers around the world with the highest quality products.
In addition to direct selling in more than 20 countries in North America, Europe and
Asia/Pacific, Miltenyi Biotec also provides support for our customers through an
extensive distributor network covering dozens of additional countries.

REAlease

®

Anti-Sca-1 Complexes

, mouse

Clone REAL175 is an antibody fragment derived from the full Anti-Sca-1 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.

Clone REAL175 recognizes the mouse stem cell antigen-1 (Sca-1) antigen, an 18 kDa glycosylphosphatidylinositol (GPI) linked protein which is also known as lymphocyte antigen 6A and 6E (Ly-6A/E). Clone REAL175 specifically recognizes both Ly-6E.1 and Ly-6A.2, which are gene products of the two Ly-6A/E alleles expressed in different mouse strains (Ly-6E.1: e.g. BALB/c, C3H, NZB; Ly-6A.2: e.g. C57BL/6, SJL, 129, AKR). Sca-1 is expressed on hematopoietic stem cells and progenitor cells in mouse bone marrow and is one of the defining markers for so-called KSL (c-kit

. Furthermore, Sca-1 is expressed on stem cells in a variety of nonhematopoietic tissues, such as adult liver, heart, and prostate.

The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Figure 1

Bone marrow cells from C57BL/6 mice were stained with REAlease Anti-Sca-1-FITC (B) or with the corresponding REAL Control (A). REAlease Anti-Sca-1-FITC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease Anti-Sca-1-FITC (D). Cells were labeled with CD117 antibodies in addition and analyzed by flow cytometry using the MACSQuant

®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.