Abstract

A resolution-enhanced integral imaging microscope that uses lens array shifting is proposed in this study. The lens shift method maintains the same field of view of the reconstructed orthographic view images with increased spatial density. In this study, multiple sets of the elemental images were captured with horizontal and vertical shifts of the micro lens array and combined together to form a single set of the elemental images. From the combined elemental images, orthographic view images and depth slice images of the microscopic specimen were generated with enhanced resolution.

Experimental results (a) single set of the elemental image captured with stationary lens array (b) reconstructed orthographic view images. Pixel count of each orthographic view is 45 × 45 and the number of view images is 15x15.

Experimental results (a) high spatial density elemental images combined from 25 sets of the elemental images that were captured by a lens array shift (b) reconstructed orthographic view images. The pixel count of each orthographic view is 225 × 225 and the number of view images is15x15.

Movie of the orthographic view images that was reconstructed by using (a) (Media 1) one set of the elemental images that was captured with a stationary lens array (Fig. 9) and (b) (Media 2) high density elemental images obtained by using lens array shifting (Fig. 10).