Ionenkanäle in deaktivierter Mikroglia

by Eder, Claudia

Abstract (Summary)

The current work describes patch clamp recordings in cultured murine microglial cells, which had been deactivated following exposure to astrocyte-conditioned medium. Deactivated microglial cells expressed inwardly rectifying, outwardly rectifying and calcium-activated potassium channels. The outwardly rectifying potassium channels were upregulated following treatment of microglial cells with the astrocyte-conditioned medium. The anti-inflammatory cytokine transforming growth factor was released by astrocytes and appeared to be responsible for the observed upregulation of outwardly rectifying potassium channels in deactivated microglia. In addition, deactivated microglial cells expressed chloride channels, which were found to be involved in microglial ramification, i.e., in the transformation of microglial cells from ameboid into ramified morphology. Voltage-gated proton channels, which are involved in processes of oxygen radical generation, were downregulated in deactivated microglial cells.