Advantages of CST Custom Conjugation:

Flexibility: Tiered options range from basic antibody conjugation to full validation and stability testing of conjugates

Validation: Conjugates are tested in key applications using biologically relevant cell systems and controls, and verified for physical integrity using size exclusion chromatography

Optimization: Service options include choice of conjugation chemistries, removal of free dye and/or antibody purification, and identification of optimal degree of labeling (DOL) to ensure the best signal-to-noise ratio

Support: Application consultation and extensive technical support from the same scientists who produce and validate our commercially available antibody conjugates

The Alexa Fluor dye conjugates in this product are sold under license from Life Technologies Corporation, for research use only excluding use in combination with DNA microarrays and high content screening (HCS). Alexa Fluor is a registered trademark of Life Technologies, Inc. Cy, Cy7, CyDye, and Sepharose are registered trademarks of GE Healthcare. Pacific Blue is a registered trademark of Molecular Probes, Inc. Dylight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.U.S. Patent No. 5,675,063.

DIY conjugation kits look good on paper, but are you getting your money’s worth? Inefficiencies caused by suboptimal labeling, interference with specificity, and/or destabilization/degradation can negatively affect performance. Another drawback: loss of antibody during conjugation steps can mean finishing with significantly less functional antibody than you started with.

With Custom Conjugation Services from Cell Signaling Technology (CST), you can avoid these time-consuming pitfalls and get the full amount of antibody you pay for.

Comparison of HRP-conjugated antibody in ELISA

Comparison of HRP conjugation of Phospho-Akt (Ser473) (193H12) Rabbit mAb conjugated using CST custom conjugation method (blue) or conjugated using a competitor’s DIY HRP conjugation kit (green), performed by ELISA assay. The relationship between the protein concentration of lysate from MCF7 cells untreated (dashed lines) or treated with human insulin-like growth factor I (solid lines) and the absorbance at 450 nm is shown (left). The signal-to-noise ratio was calculated at the 0.625 mg/ml lysate concentration (right).

Comparison of HRP-conjugated antibody in western blot

Comparison of HRP conjugation of GAPDH (D16H11) XP® Rabbit mAb using CST conjugation method or conjugated using a competitor’s DIY HRP conjugation kit. Western blot analysis of extracts from C6 and THP-1 cells were performed with concentration-matched antibody conjugates. These data demonstrate that signal specificity is better maintained with CST custom conjugation.

The following data illustrate performance of CST conjugated antibodies in a variety of applications. Note that the antibodies shown below are commercially available off-the-shelf; the same team of scientists performs conjugation and validation of both off-the-shelf and custom products.