2018 ACA CryoEM Workshop

The Cryo-EM Workshop: A Guide to High Resolution Structure Determination was held on July 21, 2018 as part of the American Crystallographic Association Annual Meeting in Toronto, Canada. Co-organized by Lori Passmore (MRC-LMB) and Wah Chiu (Stanford U), and sponsored by Douglas Instruments, Gatan, and ThermoFisher Scientific, the one-day workshop attracted over 130 attendees.

Cryo-EM has become increasingly popular among structural biologists and is now the method of choice for structure determination of proteins larger than ~200 kDa. It has been particularly successful for proteins that are difficult to crystallize, including membrane proteins, large assemblies and multi-protein complexes. The workshop provided a detailed overview of cryo-EM specimen preparation, image processing and building/refinement of atomic models, with focus on high-resolution single particle cryo-EM, and several hands-on tutorials. Materials from the workshop can be accessed here and through the links below.