2017-12-14T02:27:25ZA Simple and Easy Way to Enhance Sensitivity of Sn(IV) on Bismuth Film Electrodes with the Use of a Mediatorhttps://hdl.handle.net/10195/69560
A Simple and Easy Way to Enhance Sensitivity of Sn(IV) on Bismuth Film Electrodes with the Use of a Mediator
Tyszczuk-Rotko, Katarzyna; Metelka, Radovan; Vytřas, Karel; Barczak, Mariusz; Sadok, Ilona; Miroslaw, Barbara
For the first time an in situ-plated bismuth film electrode prepared with the use of a reversibly deposited mediator (Zn) was applied to anodic stripping voltammetry of the metal ions. This simple and easy way of the electrode surface modification by metal films was suggested to increase the voltammetric signal of Sn(IV) and, consequently, to improve the sensitivity and tin detection limit. The structural and chemical information about the bismuth films plated with and without the use of the mediator were derived from AFM and XPS data analysis.
2016-01-01T00:00:00Z{(S)-2-[({2-[1-(Anthracen-9-ylmethyl)-pyrrolidine-2-carboxamido]phenyl}-(phenyl)methylidene)amino]acetato(2&#2;)-kappa4N,N´,N´´,O1}nickel(II)https://hdl.handle.net/10195/69559
{(S)-2-[({2-[1-(Anthracen-9-ylmethyl)-pyrrolidine-2-carboxamido]phenyl}-(phenyl)methylidene)amino]acetato(2&#2;)-kappa4N,N´,N´´,O1}nickel(II)
Padělková, Zdeňka; Popkov, Alexander; Nádvorník, Milan
The title compound, [Ni(C 35H 29N 3O 3)], includes a Schiff base ligand derived from (S)-1-[(anthracen-9- yl)methyl]-N-(2-benzoylphenyl)pyrrolidine-2-carboxamide and glycine. The Ni II atom is coordinated by three N atoms [Ni - N = 1.937 (3), 1.850 (3) and 1.850 (3) Å] and one O atom [Ni - O = 1.859 (2) Å], resulting in a pseudo-square-planar coordination environment.
2012-01-01T00:00:00ZProject Management Methods in Projects Co-financed by EU Fundshttps://hdl.handle.net/10195/69558
Project Management Methods in Projects Co-financed by EU Funds
Košťálová, Jana; Tetřevová, Liběna; Paták, Michal
This paper deals with the management of projects co-financed by European Union funds (structural funds and the Cohesion Fund) in the Czech Republic (EU projects). The authors aimed to analyze and assess the scope of familiarity with basic project management methods and their application within the implementation of EU projects in the Czech Republic in the Programming Period 2007-2013. Based on a questionnaire survey of EU project organisers, the authors evaluate their attitudes to project management methods as effectiveness improvement tools. The analysis also considers the degree of dependence between familiarity with project management methods, their application, and their effectiveness as perceived by EU project organisers. Finally, it presents recommendations to the institutional authorities and project organisers with respect to applications during the Programming Period 2014-2020. We can state that project management methods were not applied to a sufficient extent. To improve the effectiveness of the implementation of EU projects, EU fund provider&apos;s methodical instructions have to include the obligations or recommendations to apply project management methods, mainly methods applicable within more project management knowledge areas and within more project life cycle stages (i.e. Logical Framework, Pre-Project Study with Formalized Structure, Formalized Risk Analysis and Formalized Project Communication Plan).
2017-01-01T00:00:00ZOn-line Monitoring of In-Vitro Oral Bioaccessibility Tests as Front-End to Liquid Chromatography for Determination of Chlorogenic Acid Isomers in Dietary Supplementshttps://hdl.handle.net/10195/69536
On-line Monitoring of In-Vitro Oral Bioaccessibility Tests as Front-End to Liquid Chromatography for Determination of Chlorogenic Acid Isomers in Dietary Supplements
Kremr, Daniel; Cocovi-Solberg, David J.; Bajerová, Petra; Ventura, Karel; Miró, Manuel
A novel fully automated in-vitro oral dissolution test assay as a front-end to liquid chromatography has been developed and validated for on-line chemical profiling and monitoring of temporal release profiles of three caffeoylquinic acid (CQA) isomers,namely, 3-CQA,4-CQA and 5-CQA, known as chlorogenic acids, in dietary supplements. Tangential-flow filtration is harnessed as a sample processing approach for on-line handling of CQA containing extracts of hard gelatin capsules and introduction of protein-free samples into the liquid chromatograph. Oral bioaccessibility/dissolution test assays were performed at 37.0±0.5 °C as per US Pharmacopeia recommendations using pepsin with activity of ca. 749,000 USP units/L in 0.1 mol/L HCl as the extraction medium and a paddle apparatus stirred at 50 rpm. CQA release rates and steady-state dissolution conditions were determined accurately by fitting the chromatographic datasets, namely, the average cumulative concentrations of bioaccessible pools of every individual isomer monitored during 200 min, with temporal resolutions of ≥10 min, to a first-order dissolution kinetic model. Distinct solid-to-liquid phase ratios in the mimicry of physiological extraction conditions were assessed. Relative standard deviations for intra-day repeatability and inter-day intermediate precision of 5-CQA within the 5–40 µg/mL concentration range were &lt;3.4% and &lt;5.5%, respectively. Trueness of the automatic flow method for determination of 5-CQA released from dietary supplements in gastric fluid surrogate was demonstrated by spike recoveries, spanning from 91.5–104.0%, upon completion of the dissolution process. The proposed hyphenated setup was resorted for evaluating potential differences in dissolution profiles and content of the three most abundant chlorogenic acid isomers in dietary supplements from varied manufacturers.
2017-01-01T00:00:00Z