This article gives a brief introduction to the Objective Structured Clinical Examination (OSCE) and analyzes developmental progress of OSCE at both home and abroad and standardized patients' application in OSCE. Also, this article expounds application of OSCE in graduation exam of clinical medical students. Finally, this article summarizes pre-exam preparation work and flow of OSCE exam in Affiliated Hospital of Clinical Medical School, analyzes OSCE and puts forward suggestions to improve OSCE.