NEB TV

Several of the most important neglected tropical diseases in humans are the result of infection with filarial nematode parasites. In this episode of NEB TV, learn more about these diseases and their impact, and how understanding their biology can help identify methods to detect, control and treat these parasites in the field.
more

Go behind the scenes into NEB’s technical support program. Hear from our scientists as they describe memorable tech support moments and interesting collaborations that have resulted from tech support calls.
more

In this episode we are talking about the importance of RNA modifications. Hear about protocols for prokaryotic transcription start site (TSS) determination, as well as detection and quantitation of RNA modifications, and learn how the RNA Modification Database can help your RNA research.
more

Learn about glycobiology and its importance in clinical and diagnostic applications in this episode of NEB TV. Also, hear more about how NEB is setting the bar for product quality in this rapidly growing field.
more

Can laboratories become more “green"? In this episode of NEB TV, find tips on how you can help to reduce your environmental footprint in the lab, and hear about some NEB initiatives, including a unique approach for wastewater treatment and the development of our new environmentally friendly DNA purification kits.
more

Whether you are new to library preparation for NGS, using reagents from another supplier, or sending samples to a core facility, it is important to know what it takes to get high-quality libraries. Learn more about library preparation and hear where next generation sequencing is going in the latest episode of NEB TV.
more

In Episode 3, learn more about amplification, including new techniques to bring amplification into the field. Also, learn more about high-fidelity DNA polymerases, and how we responded to a recent tech support question on the subject.
more

Passion in Science Awards

Shelly Xie shares her passion for science, art and humanity. Shelly illuminates personal stories of global health issues through sand animation. Enjoy this premiere of Shelly’s work created in collaboration with Pan American Health Organization (PAHO).
more

Professor Chris Martine shares his passion for inspiring exploration and discovery in botany and the natural world. Chris connects with students at Bucknell University and beyond with humor and science-rich content through his video series “Plants are Cool, Too!”.
more

Professor Amanda Munson of Shenandoah University shares her passion for inspiring girls to pursue careers in STEM fields. Amanda describes GO GIRL™ (Genomic Opportunities for Girls in Research Labs), an education outreach program designed to boost confidence and provide hands-on science experience to high school girls in a research laboratory with state-of-the-art equipment.
more

David Ng, Director of the Science Literacy program at Michael Smith Labs of the University of British Columbia, shares his passion for public understanding of science. David explains how he promotes science communication through workshops, educational tools and research studies, and suggests that all scientists find a genuine way to advocate science and engage the public.
more

Saban Ul-Hasan, graduate student at University of California Merced, shares her passion for inspiring understanding of symbiotic relationships between scientists, artists, the public and the environment. Sabah promotes these relationships through production of the educational video series “Biota”, which highlights specific ecosystems and features music of local bands and work of local artists.
more

Karishma Bhagani, undergraduate student at University of New York, shares her passion for providing access to clean drinking water in countries suffering from water scarcity. Karishma created the aspiring non-profit organization Matone de Chiwit, which provides economical water purification units comprised of domestically available elements to rural communities.
more

Nina Dudnik shares her passion for providing global access to scientific research equipment and collaboration. Nina founded Seeding Labs to support scientific discovery and education in the developing world through financial resources, research equipment and access to expertise. She explains how providing repurposed equipment has allowed labs to educate the next generation of scientists, secure funding to support future research, and empower scientists in developing countries.
more

Lucia Prieto Godino shares her passion for providing access to science education and hands-on training in Africa. Lucia founded TReND in Africa to support global science efforts through educational course training, equipment donations and encouragement of international collaboration.
more

Scott O’Neill shares his passion for developing novel approaches to controlling mosquito transmitted viruses by introducing Wolbachia infections in mosquitos. Scott describes the troubling rise in mosquito-borne viruses, and offers the promising results of his work with Eliminate Dengue.
more

Lisa Anderson, post-doc at MIT, shares her passion for making the world a better place through science and technology. Lisa discusses the laboratory glove recycling program that she helped to create while at University of California Davis. She also describes green lab assessment programs and her current efforts at MIT.
more

Lucy Lahrita, of Hokkaido University, Japan, shares her passion for protecting the environment by utilizing chemical recycling to create synthetic fuel from plastic waste. Lucy describes how specific types of plastic can be used to not only create crude oil, but a new job sector as well. She encourages us all to think globally and act locally to protect our environment.
more

Alexandra Polasko, graduate student at UCLA, shares her passion for providing clean drinking water through the process of bioremediation. Alexandra explains how bioremediation uses microbes to degrade contaminants and describes her development of lost cost, rapid indicator technology to identify naturally occurring bacteria capable of degrading hazardous pollutants.
more

Scott Chimileski, research fellow at Harvard Medical School, shares his passion for communicating the value of microbes through photography. Scott discusses the book he is currently co-authoring with Roberto Kolter and he describes the exhibit he is designing for Harvard Museum of Natural History, both of which aim to communicate the beauty, history and power of microbes through engaging photography.
more

Christine Liu, graduate student at University of California Berkeley, shares her passion for communicating complex science topics to a broad audience through zines. Christine co-founded Two Photon Art collective, and she describes how the informal presentation style of zines makes science more approachable and less intimidating.
more

Will Ryan, graduate student at Florida State University in Tallahassee, shares his passion for communicating seminal scientific literature in engaging short films. Will distills the take-home messages of the literature and delivers these messages through poetry and art in his films, making science literature less intimidating and more relatable for his audience.
more

Dana Simmons, graduate student at The University of Chicago, shares her passion for sharing the beauty of science and the complexity of the human neural system through photography. Dana explores the similarities between nature and biology by introducing fluorescent dye into purkinje cells and capturing striking images after the completion of her scientific experiments with the cells. Dana elevates her science to art form and reaches a broader audience with her efforts.
more

The questions posed to NEB tech support run the gamut from basic procedural and historical questions to complex issues about cutting-edge applications. Our scientists are ready and willing to help!more

Nina Dudnik shares her passion for providing global access to scientific research equipment and collaboration. Nina founded Seeding Labs to support scientific discovery and education in the developing world through financial resources, research equipment and access to expertise. She explains how providing repurposed equipment has allowed labs to educate the next generation of scientists, secure funding to support future research, and empower scientists in developing countries.more

Saban Ul-Hasan, graduate student at University of California Merced, shares her passion for inspiring understanding of symbiotic relationships between scientists, artists, the public and the environment. Sabah promotes these relationships through production of the educational video series “Biota”, which highlights specific ecosystems and features music of local bands and work of local artists.more

Lucia Prieto Godino shares her passion for providing access to science education and hands-on training in Africa. Lucia founded TReND in Africa to support global science efforts through educational course training, equipment donations and encouragement of international collaboration.more

Lucy Lahrita, of Hokkaido University, Japan, shares her passion for protecting the environment by utilizing chemical recycling to create synthetic fuel from plastic waste. Lucy describes how specific types of plastic can be used to not only create crude oil, but a new job sector as well. She encourages us all to think globally and act locally to protect our environment.more

Shelly Xie shares her passion for science, art and humanity. Shelly illuminates personal stories of global health issues through sand animation. Enjoy this premiere of Shelly’s work created in collaboration with Pan American Health Organization (PAHO).more

Will Ryan, graduate student at Florida State University in Tallahassee, shares his passion for communicating seminal scientific literature in engaging short films. Will distills the take-home messages of the literature and delivers these messages through poetry and art in his films, making science literature less intimidating and more relatable for his audience.more

Scott Chimileski, research fellow at Harvard Medical School, shares his passion for communicating the value of microbes through photography. Scott discusses the book he is currently co-authoring with Roberto Kolter and he describes the exhibit he is designing for Harvard Museum of Natural History, both of which aim to communicate the beauty, history and power of microbes through engaging photography.more

Scott O’Neill shares his passion for developing novel approaches to controlling mosquito transmitted viruses by introducing Wolbachia infections in mosquitos. Scott describes the troubling rise in mosquito-borne viruses, and offers the promising results of his work with Eliminate Dengue.more

Lisa Anderson, post-doc at MIT, shares her passion for making the world a better place through science and technology. Lisa discusses the laboratory glove recycling program that she helped to create while at University of California Davis. She also describes green lab assessment programs and her current efforts at MIT.more

Professor Chris Martine shares his passion for inspiring exploration and discovery in botany and the natural world. Chris connects with students at Bucknell University and beyond with humor and science-rich content through his video series “Plants are Cool, Too!”.more

Christine Liu, graduate student at University of California Berkeley, shares her passion for communicating complex science topics to a broad audience through zines. Christine co-founded Two Photon Art collective, and she describes how the informal presentation style of zines makes science more approachable and less intimidating.more

Alexandra Polasko, graduate student at UCLA, shares her passion for providing clean drinking water through the process of bioremediation. Alexandra explains how bioremediation uses microbes to degrade contaminants and describes her development of lost cost, rapid indicator technology to identify naturally occurring bacteria capable of degrading hazardous pollutants.more

Professor Amanda Munson of Shenandoah University shares her passion for inspiring girls to pursue careers in STEM fields. Amanda describes GO GIRL™ (Genomic Opportunities for Girls in Research Labs), an education outreach program designed to boost confidence and provide hands-on science experience to high school girls in a research laboratory with state-of-the-art equipment.more

Karishma Bhagani, undergraduate student at University of New York, shares her passion for providing access to clean drinking water in countries suffering from water scarcity. Karishma created the aspiring non-profit organization Matone de Chiwit, which provides economical water purification units comprised of domestically available elements to rural communities.more

Dana Simmons, graduate student at The University of Chicago, shares her passion for sharing the beauty of science and the complexity of the human neural system through photography. Dana explores the similarities between nature and biology by introducing fluorescent dye into purkinje cells and capturing striking images after the completion of her scientific experiments with the cells. Dana elevates her science to art form and reaches a broader audience with her efforts.more

David Ng, Director of the Science Literacy program at Michael Smith Labs of the University of British Columbia, shares his passion for public understanding of science. David explains how he promotes science communication through workshops, educational tools and research studies, and suggests that all scientists find a genuine way to advocate science and engage the public.more

In Episode 3, learn more about amplification, including new techniques to bring amplification into the field. Also, learn more about high-fidelity DNA polymerases, and how we responded to a recent tech support question on the subject.more

Whether you are new to library preparation for NGS, using reagents from another supplier, or sending samples to a core facility, it is important to know what it takes to get high-quality libraries. Learn more about library preparation and hear where next generation sequencing is going in the latest episode of NEB TV.more

Go behind the scenes into NEB’s technical support program. Hear from our scientists as they describe memorable tech support moments and interesting collaborations that have resulted from tech support calls.more

Several of the most important neglected tropical diseases in humans are the result of infection with filarial nematode parasites. In this episode of NEB TV, learn more about these diseases and their impact, and how understanding their biology can help identify methods to detect, control and treat these parasites in the field.more

As a scientist, there is nothing worse than running out of your favorite enzyme in the middle of an experiment. NEBnow’s self-service kiosk enables our products and your discoveries to always be within reach.more

Can laboratories become more “green"? In this episode of NEB TV, find tips on how you can help to reduce your environmental footprint in the lab, and hear about some NEB initiatives, including a unique approach for wastewater treatment and the development of our new environmentally friendly DNA purification kits.more

The common problem of adaptor dimer formation during small RNA library construction can be avoided by using NEBNext® protocols. Learn about this technique, and how it improves both performance and sensitivity in library construction.more

Target enrichment offers several advantages over more comprehensive genomic profiling for a variety of scientific and applications. During this webinar, we explore the advantages and limitations of target enrichment, and discuss how NEBNext Direct®, a novel solution for selective enrichment of genomic targets, addresses these challenges.more

PCR, Polymerases & Amplification Technologies

NEB offers a variety of DNA polymerases for all of your experimental needs. High-fidelity, multiplex, and fast PCR are just a few of the types of amplification reactions you may need to conduct, and Breton explains which polymerases to choose.more

Type II restriction enzymes are most commonly used for molecular biology applications, as they recognize stereotypical sequences and produce a predictable cleavage pattern. Learn more about how Type II REs work.more

Droplet digital PCR is a method for accurately quantitating copies of DNA or RNA in a sample. Each PCR reaction is separated into thousands or millions of droplets for analysis. Learn more about droplet digital PCR.more

Here we illustrate the use of glycosidases for the analysis of a model glycoprotein: recombinant human chorionic gonadotropin beta (hCGβ), which carries both N-glycans and O-glycans in this video. The technique requires only simple instrumentation and typical consumables, and it can be readily adapted to the analysis of multiple glycoprotein samples.more

Learn about the core sequences and common modifications of N-linked and O-linked glycans in this video.
Analysis of these glycans and/or peptide portions of the glycoprotein can be accomplished with the use of deglycosylation enzymes, which are explained in detail. Unlike other chemical deglycosylation methods, enzymatic treatment is much gentler and can provide complete sugar removal with no protein degradation.more

NEB and NIBRT scientists devised a robust workflow for comprehensive profiling of GSL head group glycans, which has the potential to facilitate functional glycomics studies and biomarker discovery. Chris shares details of this recent publication.more

Learn about glycobiology and its importance in clinical and diagnostic applications in this episode of NEB TV. Also, hear more about how NEB is setting the bar for product quality in this rapidly growing field.more

Cellular Analysis

View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells.more

Gene Expression

View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells.more

DNA Modifying Enzymes & Cloning Technologies

PCR Cloning is an easy and reliable cloning method. The name is derived from the use of a DNA amplification step to generate the amplicon. Learn more about the benefits and disadvantages of PCR Cloning.more

Gibson Assembly™ employs three enzymatic activities in a single-tube reaction: 5´ exonuclease, the 3´ extension activity of a DNA polymerase and DNA ligase activity. The 5´ exonuclease activity chews back the 5´ end sequences and exposes the complementary sequence for annealing. The polymerase activity then fills in the gaps on the annealed regions. A DNA ligase then seals the nick and covalently links the DNA fragments together. The overlapping sequence of adjoining fragments is much longer than those used in Golden Gate Assembly, and therefore results in a higher percentage of correct assemblies. The NEB Gibson Assembly Master Mix (NEB #E2611) and Gibson Assembly Cloning Kit (NEB #E5510S) enable rapid assembly at 50˚C.more

Phosphorylation is the process by which phosphate groups are added to a molecule by a kinase. The phosphorylation status of a fragment of DNA can influence its ability to proceed in reactions. Learn more about phosphorylation and kinases.more

The first step in determining how your ends will be blunted is to determine if they are 5´ or 3´ overhangs. This tutorial will teach you how to identify what type of overhang you have, as well as which enzyme will blunt that end, and how.more

Restriction enzymes are an integral part of the cloning workflow, for generating compatible ends on fragments and vectors. This animation discusses three guidelines for determining which restriction enzymes to use in your cloning experiment.more

Dephosphorylation is the process by which phosphate groups are removed from a molecule by a phosphatase. Removal of phosphate groups from a DNA fragment can prevent ligation. Learn more about dephosphorylation and phosphatases.more

Transformation is the process by which bacteria are made to take up exogenous DNA. The word is derived from Griffith's discovery of a "transforming principle". Learn more about transformation and how it is used in cloning workflows.more

Using PCR, restriction sites are added to both ends of a dsDNA, which is then digested by the corresponding REases. The cleaved DNA can then be ligated to a plasmid vector cleaved by the same or compatible REases with T4 DNA ligase. DNA fragments can also be moved from one vector into another by digesting with REases and ligating to compatible ends of the target vector.more

High fidelity polymerases are everywhere—but why would you need a high fidelity ligase? And what do we even mean by “fidelity” when we’re talking about ligation? In this webinar, NEB Scientist and ligase expert Greg Lohman discusses mismatch ligation by DNA ligases and the molecular diagnostics applications that depend on the use of high-fidelity DNA ligases like NEB’s HiFi Taq DNA Ligase to detect single base differences in DNA.more

Whether you are new to cloning or a seasoned expert, we have the webinar for you. NEB’s resident cloning experts provided an overview of various cloning methods, and answered common cloning questions received from customers.more

Traditional Cloning refers to the generation of DNA fragments using restriction enzymes, and their subsequent assembly and transformation. The name is derived from the method’s history as the first widely-accepted cloning method. Learn more about the benefits and disadvantages of Traditional Cloning.more

Epigenetics

Cancer cells have epigenetic changes to their DNA. One of these causes the turning off (silencing) of tumor suppressor genes, a small set of genes involved in cell cycle control. Sriharsa discusses drugs currently in the clinic that are able to demethylate DNA and improve the lives of cancer patients.more

Epigenetic changes to DNA impact a wide range of biological processes such as organism development and disease progression. Watch as Sriharsa enthusiastically describes this exciting and complex field that has the power to "re-write" the course of DNA transcription.more

New England Biolabs is pleased to introduce the EpiMark® 5-hmC and 5-mC Analysis Kit, a simple and robust method for the identification and quantitation of 5-hydroxymethylcytosine (5-hmC) and 5-methylcytosine (5-mC) within a specific DNA locus.

Watch an interactive tutorial explaining the different molecular mechanisms by which epigenetic change influences gene expression. Learn about how NEB’s line of EpiMark® validated reagents are targeted to the various enzymes and DNA elements that are altered by epigenetic change.more

Curious about the role of 5-hydroxymethylcytosine in the genesis and function of the epigenome? Watch and learn as Sriharsa explains the study of 5-mC and 5-hmC as markers of epigenetically-modified genomic DNA, and the best methods for differentially detecting these modified species.more

If all cells are created from the same genetic material, why are there so many different cell types? Listen to Sriharsa Pradhan, Senior Scientist, RNA Biology at NEB, as he describes how DNA is methylated and how this affects the path of reading the DNA code the same way an obstruction would derail a train off its tracks.more

Rick Feehery, Research Associate at New England Biolabs, Inc, explains the mechanisms of epigenetic DNA modification. To drive home the power of such tiny changes, he uses the example of honeybee colonies, wherein DNA methylation is the only factor that determines whether a bee is born a worker, drone or queen.more

Behind the paper: Sriharsa and Chaithanya summarize their recent Genome Biology publication describing the NicE-seq method for open chromatin profiling and the advantages it offers over current chromatin accessibility profiling techniques.more

RNA Reagents

This method describes high yield in vitro synthesis of both capped and uncapped mRNA from a linearized plasmid containing the Gaussia luciferase (GLuc) gene. The RNA is purified and a fraction of the uncapped RNA is enzymatically capped using the Vaccinia virus capping enzyme. In the final step, the mRNA is transfected into HeLa cells and cell culture supernatants are assayed for luciferase activity.more

In this episode we are talking about the importance of RNA modifications. Hear about protocols for prokaryotic transcription start site (TSS) determination, as well as detection and quantitation of RNA modifications, and learn how the RNA Modification Database can help your RNA research.more

In this webinar you will learn how to increase editing efficiency by directly introducing Cas9 ribonucleoproteins (RNPs) to cells through electroporation or lipofection. Rapid sgRNA synthesis requiring only a single user-supplied ~55mer single-stranded DNA oligonucleotide is described. Methods for assessing genome editing efficiency will be discussed including T7-endonuclease I-based methods, sequencing-based methods, and in vitro Cas9 digestion.more

Buffers

Traditional Cloning refers to the generation of DNA fragments using restriction enzymes, and their subsequent assembly and transformation. The name is derived from the method’s history as the first widely-accepted cloning method. Learn more about the benefits and disadvantages of Traditional Cloning.more

As a scientist, there is nothing worse than running out of your favorite enzyme in the middle of an experiment. NEBnow’s self-service kiosk enables our products and your discoveries to always be within reach.
more

The questions posed to NEB tech support run the gamut from basic procedural and historical questions to complex issues about cutting-edge applications. Our scientists are ready and willing to help!
more