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相关性Complex I, or NADH ubiquinone oxidoreductase, is a large protein complex of 950,000 Da molecular weight made up by 45 to 46 different subunits. A total of seven of the subunits of the complex are encoded by mitochondrial DNA, while the remainder subunits are nuclear encoded, which are translated in the cytosol and translocated into the organelle for assembly at the inner membrane.
The enzyme complex catalyses electron entry from NADH via a flavin (FMN) and several non-heme iron centers. Complex I is sensitive to a wide range of inhibitors, many of which are pesticides or other common environmental toxins, such as rotenone. Complex I dysfunction is a common cause of genetic OXPHOS defects. Altered functioning of this complex is also thought to contribute to several neurological disorders including Parkinson’s disease and schizophrenia. Also, there is evidence of Complex I involvement in diabetes.

Anti-Complex I Immunocapture antibody [18G12BC2] 图像

Immunocytochemistry image of ab109798 stained fibroblasts cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the antibody (ab109798, 1 µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 4884 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondria.

- Anti-Complex I Immunocapture antibody [18G12BC2] (ab109798)

HL-60 cells were stained with 1 µg/mL Complex I antibody ab109798 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

The image is not of a blot but of a gel stained for total protein. The annotations refer to subunits of mouse brain mitochondria Complex I, identified by mass spec as described in the reference at this link: http://www.mcponline.org/content/4/1/84.full...

This antibody is an immunocapture antibody which can pull down the whole complex I from a given sample. It works also as a ICC and Flow antibody. It's a weak WB antibody, light up a 8kda subunit of complex I but we have difficulty to identify which sub...

Thank you very much for your phone call. I have had discussion with my colleague in lab. They have suggested that the antibody was tested only with lysates and cells as presented on the datasheet. No other tested data is available for this product...

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I am not sure which publication you are looking at however I have the following publication from Sashi Nadanaciva;
http://www.mitosciences.com/PDF/MitoSciences_OXPHOS_Screen_Paper.pdf
In this article MS101c is u...

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I suppose you have a question about ab109798; Anti-Complex I Immunocapture antibody. This antibody detects the presence of Complex I in cell sample; this unfortunately is not suitable for assaying the enzymatic activity...

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Please click these links to find the suitable antibodies. Type the MS code in Abcam search box to get the Abcam catalogue number.
Complex I
http://www.mitosciences.com/complex_i.html
Complex IV
http://www.mi...