Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

Concentration

0.2 mg/ml

General Readings

Gupta K, et al. (1998) Biochim Biophys Acta, 61789:1-11.

Storage

Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.Shelf life: one year from despatch.

Format

Purification:

Protein G chromatography

Buffer System:

10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide.

State:

Liquid ascites fluidPurified

Specificity

Specificity:

This antibody detects Thrombospondin.It reacts with a 25 kDa peptide (heparin-binding domain) from thermolysin digests of TSP that is not disulfide bonded to any other region of the TSP molecule. Heparin efficiently inhibits the binding to TSP.