アプリケーション

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション

Abreviews

特記事項

ELISA

Use at an assay dependent concentration.

IHC-Fr

1/250.

WB

1/250. Predicted molecular weight: 84 kDa. using HRP or AP. With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.

ICC

Use at an assay dependent concentration.

ターゲット情報

機能

This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

組織特異性

Expressed in Purkinje cells of the cerebellar cortex.

関連疾患

Defects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA).

Thank you for your technical support and interest. I would suggest applying longer incubation time (overnight instead of 1 hr). Customer may need to use higher final concentration of this primary antibody and longer permeabilization would be also ...

I am sorry to hear this customer has been experiencing problems with one of our PKC gamma antibodies. The quality of our products is important to us and I would like to reassure you that we investigate all custom...

Thank you for your e-mail.
It is good to know that the customer uses a good lysis buffer, secondary antibody and detection system.
However, the form you have sent us states the customers used NRK，NR8383, SMC and HSC-T6 cell lines, which are not neuro...

I can confirm that we recommend a positive control of recombinant protein or as PKC gamma is expressed solely in the brain and spinal cord and its localization is restricted to neurons to try brain or spinal cord lysate, my apologies for the delay in c...

Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with this antibody. Although ab4145 is predicted to react with mouse, it has not yet been tested so we are unable to guarantee results. The antibody is guaranteed to ...

Thank you for your patience and for the details that you have provided. What was the lot number (located on the vial) that you received? What was the Abcam order number or purchase order number that was used? We haven't had any other complaints but I w...