Wild edible mushroom are the special product of the forest. The diversity of mushroom and their natural beauty inhabit major threat in the biological world and India has been a frame for these fungi. The previous attempts for genetic improvement in mushrooms had little success due to low genetic diversity amongst commercially cultivated mushrooms strains and non- inclusion of wild collections in the breeding programs. This limited availability of genetic variation significantly slowed down the progress of genetic improvement in this commercially most important mushroom. The present investigation of genetic diversity of Pleurotus species were ranged from 30 to 70%. The Cluster analysis were using Unweighted Pair-Group Method with Arithmetic Mean (UPGMA), clearly separate dendrogram was obtained from the molecular marker systems for all the eight Pleurotus species. Clustering on the basis of Random Amplified Polymorphic DNA (RAPD) marker using Jaccard’s coefficient generated two (I and II) broad groups of six and two Pleurotus species. Clusters also confirmed 70% of genetic diversity among the Pleurotus species.