AbstractStem cells application in neural system injuries is an actual and prospective scientific field of modern regenerative medicine. In recent years much attention has been paid for study of regenerative effects of multipotent mesenchymal stromal cells (MMSCs) from different sources on injured tissues.The aim of our study was to determine the level of tissue damage in hippocampus after in vitro model of ischemia and to investigate the effect of bone marrow MMSСs in non-contact co-culture with ischemic neural tissue. The ischemic injury of neural tissue in vitro was modeling in organotypic hippocampal slice culture (OHCs) by oxygen-glucose deprivation (OGD).
Immunohistochemical analysis after 24 hours of BM-MMSCs co-cultivation with OHCs after ischemia showed a significant reduction of caspase-3-positive dead neural cells, as compared to those in ischemic damage without BM-MMSCs co-cultivation, and reducing of glial cells activation. After co-cultivation of OHCs after OGD with BM-MMSCs there remained cytoarchitectonics of the neural tissue.Analyzing of our data, the neuroprotective effects of BM-MMSCs in non-contact co-cultivation with ischemic hippocampal organotypic slice culture is shown.

25. Winkelmann ER, Charcansky A, FaccioniHeuser MC. An ultrastructural analysis of cellular death in the CA1 field in the rat hippocampus after transient forebrain ischemia followed by 2, 4 and 10 days of reperfusion. Anat. Embryol. (Berl). 2006;211(5):423-434.https://doi.org/10.1007/s00429-006-0095-z
PMid:16673115