Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

PMLTEST305B - Perform aseptic techniques.

Course Description

This unit of competency covers the ability to prepare primary tissue cultures for applications, such as maintenance of animal cell lines and propagation of plants by tissue culture and basic subculture procedures. Personnel are required to manipulate equipment and materials and samples to prevent contamination at all preparation stages. They will have ready access to enterprise procedures and will work under direct supervision.

4.1 Incubate culture in specified conditions4.2 Monitor growth of culture and record appearance and characteristics4.3 Report presence or absence of contamination4.4 Subculture the culture to continue the cell line4.5 Dispose of biohazardous and other laboratory waste safely

Element:

Prepare and test cell and tissue culture media

Performance Criteria:

2.1 Select and confirm media specifications and processes/methods2.2 Prepare culture media to suit the application2.3 Sterilise culture media and check for sterility2.4 Perform quality control checks to ensure that culture media is fit for purpose2.5 Store culture media in accordance with specifications

1.1 Ensure work practices and personal actions conform to regulations, codes, guidelines and enterprise quality assurance procedures1.2 Identify hazards and enterprise controls associated with the sample, preparation methods, reagents and equipment1.3 Select, fit and use personal protective clothing and safety equipment1.4 Address hazards and incidents as they arise1.5 Maintain a chain of custody, traceable to the worker, for all cells and tissues

Term one test worth 27.5 % of the total courseTerm two test worth 27.5% of the total coursePractical test at the end of term two is worth 5% of the courseEssay due the 11.05.10 is worth 12.5% of the courseTalk on 11.05.10 is worth 12.5% of the coursePractical report one due on 02.03.10 is worth 5% of the whole coursePractical report two due on 13.04.10 is worth 10% of the course

Please not that this information is subject to change

Assessment Matrix

Other Information

Important Information

Submission of work & late work- Students are required to submit work on the due date at the School Office using the cover sheets provided. The work will be accepted through the School Office and you will receive a receipt which you should keep as proof of submission. Students must NOT submit work directly to the teacher.

Late submission of work will incur a penalty of 5% of the maximum mark per day unless Special Consideration is applied for in writing using the required form. Marks can be deducted until the minimum pass mark is reached, after which no further marks will be deducted. Work will not be accepted more than 1 week after the due date.

Special Consideration- Any student who, because of illness or other circumstances beyond their control, has been prevented from attending a test, or whose performance in a test was affected by illness or other circumstances beyond their control may apply for Special Consideration. This must be done within 48 hours of the missed assessment/ assignment by lodging the appropriate form (available at the 6th floor office) with medical certificate or other supporting documentation at the Reception Office on Level 6, Building 51. Note: It is the policy of the School of Life and Physical Sciences NOT to AUTOMATICALLY PROVIDE SUPPLEMENTARY OR DEFERRED EXAMS. You will be advised of the outcome of your application for Special Consideration. The taking of Annual Leave does NOT constitute grounds for special consideration.

Attendance Requirements- Student must attend 80% of practical classes to pass. Practical exercises will be chosen for presentation as formal reports. If they are not satisfactory, you will be given an opportunity to resubmit. If you are absent from a session nominated for a formal report and you have applied for special consideration, it is your responsibility to negotiate a replacement report with the teacher. You MUST submit the number of required reports in order to pass the subject.

Plagiarism- Plagiarism is the presentation of the work, idea or creation of another person as though it is your own. It is a form of cheating and is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited.

It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or to copy your own work. Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher.

Students must follow all the OH&S rules for working in a PC2 laboratory