Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

33 kDa (note)

Positive Controls

mouse brown adipose

Form Supplied

Liquid

Concentration

0.3
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Purified by antigen-affinity chromatography.

Specifications: UCP1 antibody

Full Name

uncoupling protein 1 (mitochondrial, proton carrier)

Background

Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat. [provided by RefSeq]

Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with UCP1 antibody (GTX112784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with UCP1 antibody (GTX112784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.