Gene Pulser® Electroporation Buffer

Overview

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Bio-Rad Laboratories has helped scientists deliver molecules into cells for over 35 years. Instruments, including the Gene Pulser® series of electroporators, the MicroPulser™ electroporator, and the Helios® gene gun and PDS-1000/He™ biolistic systems, have been used successfully to transfect prokaryotic and eukaryotic cells, including plant and animal cells. These transfection instruments are cited extensively in the literature. Our chemically mediated methods include lipid-based transfection reagents — TransFectin™ lipid reagent for delivery of plasmid DNA and siLentFect™ lipid reagent for siRNA delivery.

Optimization of electroporation conditions. Human primary fibroblast cells denoted as FS (foreskin) were used to compare a square waveform with varying voltages (top) to an exponential waveform with varying capacitance values (bottom) in a 96-well plate. Although the exponential waveform resulted in good data overall, the square waveform resulted in the highest transfection efficiency and cell viability when compared side by side on the same plate. RLU, relative luminescence units.

Cell survival under a range of electroporation conditions. Post-electroporation survival of neutrophils was assayed after 24 hr in culture by counting the number of intact cells that excluded trypan blue, and dividing by the number of neutrophils initially transfected. Cell survival was substantially reduced at 300 V and 1,000 µF.

Works with most electroporators, including the Gene Pulser MXcell, Gene Pulser Xcell, and Gene Pulser II electroporation systems

Delivers siRNA, DNA, and other biomolecules into primary cells and difficult-to-transfect cell lines

Improves efficiency of transfection and maintains cell viability after electroporation

Can be used for transfection in single cuvettes or in multiwell electroporation plates

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