Figure 2: Immunofluorescent staining of the scaffolds after culturing for 1, 2, and 3 weeks in vitro for evidence of vasculature ((a)–(c)). After one week of culturing (a), CD31 positive cells (red) are detected within the scaffold. After two weeks of culturing (b), colocalization of CD31 (red) and vWF (green) positive cells are visualized. After three weeks of culturing (c), more extensive colocalization of CD31 and vWF is seen. A BrdU assay was performed in vitro after 10 days of culturing ((d)–(f)). BrdU positive nuclei (red) were seen revealing that proliferation was occurring throughout the scaffold (e).