Re: HIER on bone tissue

Hi,
Bone tissue is always a trick. My best suggestions are: 1) Don't let
the sections sit on the float bath long. 2) Don't use tissue adhesives.
(albumin, stay on etc.) 3) Use (+) charged slides. 4) Let the slides dry
as long as possible in a 60 deg. oven. 5) Don't agitate the slides while
they are heating and cooling. 6) Let them cool before rinsing them
GENTLY.
best of luck,
Amos Brooks
Efrain Pacheco wrote:
> Does anybody has any procedure to do HIER (heat
> induced epitope retrival)on bone tissue without
> loosing the cortical bone? I have two antibodies that
> appear to work well after microwave HIER except for
> the fact that the hard bone comes off the slide,
> apparently because of the heat. By the way, we are
> working with rat femurs. I will appreciate any
> suggestions.
>
> Efrain Pacheco, HT(ASCP)
> Histology Associate
> Amgen Inc.
>
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