In this study,Eucommia ulmoides calli for 18d under white light(light intensity was 12 000 lux,16 h light and 8 h darkness)were cultured and 24 h darkness culture was as control.Then transcriptome high-throughput sequencing for the Eucommia ulmoides calli were performed using the Illumina HiSeq 2500 sequencing platform.Functional annotation and enrichment analysis of differentially expressed genes were also carried out.Effect of white light on the content of chlorogenic acid of Eucommia ulmoides calli was studied.The results showed de novo assembly generated 62 030 unigenes.25 417(40.98%)annotated unigenes were obtained by selecting BLAST parameter(E-value ≤10)and HMMER parameter(E-value ≤10).Metabolic pathway analysis revealed that 3 unigenes were predicted to be responsible for the chlorogenic acid,the 3 unigenes were up-regulated and in the calli under light culture,which was meaning that the production capacity of chlorogenic acid increased.The three enzymes dominated chlorogenic acid production in Eucommia ulmoides calli.The three enzymes were phenylalanine ammonia-lyase(EC 4.3.1.24,PAL),trans-cinnamate 4-monooxygenase(EC 1.14.13.11,C4H)and Shikimate o-hydroxyl-cinnamoyltransferase(EC 2.3.1.133,HCT).The study showed that the white light could improve the production capacity of chlorogenic acid components of Eucommia ulmoides calli and represents a valuable resource for future genomic studies on Eucommia ulmoides.