This study was aimed to evaluate the performance of G. verrucosa seaweed seed produced from tissue culture and cultivated at the seaweed production center in Murante Village Suli District Luwu Regency South Sulawesi in 2014. The seeds were cultivated for three production cycles, with each cycle lasted for 40 days. The first culture period was conducted in 1.2 ha, the second in 2 ha, and the third in 4 ha of pond areas. The treatments consisted of two different seaweed seed sources, i.e: tissue cultured seaweed seed and local seaweed as a control. The seaweed stocking density used was 1.000 kg/ha cultivated using broadcast method. The daily growth rate (DGR) and biomass as production indicators were measured at the initial and the end of the culture period. The agar yield was measured after harvest. The data were analyzed descriptively and presented in graphical visualization and data tabulation. The present study showed that tissue cultured seaweed had a higher performance in terms of growth, biomass production, and agar yield with the values of 12.1%, 17.8%, and 6.7%, respectively, than that of the local seedling. The highest DGR was produced at the second cycle, and the highest agar yield was measured at the third cycle for both of seaweed seedlings. Given this result, tissue cultured seaweed can be an alternative source of quality seeds.