アプリケーション

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション

Abreviews

特記事項

ICC/IF

WB

ELISA

追加情報ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 57 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

ターゲット情報

機能Transcriptional activator which is processed and activated during endoplasmic reticulum stress late phase. Binds to the cAMP response element (CRE) and activates transcription through CRE. Regulates the transcription of unfolded protein response target genes, preventing accumulation of unfolded proteins in damaged neurons. Also regulates the expression of SEC23A, accelerating protein trafficking from the ER to the Golgi thereby playing a key role in chondrocyte differentiation and formation of epiphyseal cartilage.

組織特異性Widely expressed with highest levels in placenta, lung, spleen and intestine, and lowest levels in heart, brain, skeletal muscle, thymus, colon and leukocytes. In fetal tissues, the weakest expression is detected in brain and heart.

翻訳後修飾Controlled by regulated intramembrane proteolysis (RIP). Following ER stress a fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases (PS1 and PS2).

Thank you for submitting your Abreview of ab76856. I am sorry to hear it did not work as expected. If you have purchased this antibody within the past 6 months or so I would be happy to offer a replacement, credit or refund. Please include the purchase...

I would be happy to a replacement CREB3L2 antibody. The only other one we have in our catalogue is ab102989, would this one be suitable for you? Also to process the replacement, could you please provide me with the original order number?

Thank you for contacting Abcam and providing your protocol and western blot image.
I have looked over your protocol and the image that you sent. Your protocol looks to be fine and you have tried multiple variations to resolve this issue. For the weste...

I am sorry that you are experiencing problems with this antibody in western blot.
According to the data sheet, this antibody has only been tested against the recombinant fragment used as immunogen and has not been tested on endogenous protein levels. ...

Predicted molecular weight of the recombinant fragment (immunogen sequence plus 26kDa tag) is 37kDa. This is what is shown on the WB images on the datasheet.
The predicted size of the protein CREB3L2 is taken from information of the swissprot s...