I'm using the 3224 scope with the "Save on Trigger" mode of the R5 SW.
(saving thousands of files)
I need to use the Save on Trigger, and I also need the R5 SW User Interface. I cannot use the R6 SW for my application.

Hi unfortunately these features will not be added to PicoScope 5 as in the foreseeable future it will be superseded with PicoScope 6.

With PicoScope 6 you can use the waveform buffer, you can create a waveform buffer to have up to 1000 frames (under preferences). You would also have to choose Repeat as your triggering. This way every time a signal is triggered it will be saved on the waveform buffer until it fills up all 1000 frames, make sure you click on stop once you collected all your data as it will cycle round.