Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

ターゲット情報

機能Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

関連疾患Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.

配列類似性Belongs to the MIF family.

細胞内局在Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.

The monoclonal antibody ab14575 was raised using human MIF, recombinant protein expressed in E. coli. The epitope has not been
determined.
For a sandwich ELISA, I recommend ab14575 for capture and goat polyclonal ab14574 for detection, followed by ...

Thank you for your enquiry.
I have found reference to the use of LPS stimulated RAW cell lysate, Neuro2A (mouse neuroblastoma cell line) lysate or a Human Thymus lysate for use as a positive control for antibodies against MIF.
Unfortunately we do...

Thank you for your email. If you are interested in ordering multiple vials of the same antibody, I can offer you a discount. However, if you are interested in ordering one each of the 5 antibodies that you listed, I cannot offer you a discount. Shippin...