In this study the relationship was explored between general
immune reactivity, as estimated by the cutaneous basophil
hypersensitivity reaction (CBHR) to phytohemagglutinin (PHA), and the
level of anti-sperm antibodies of the Ig A and Ig G class in the cervical
mucus and sera of cows with different reproductive results. The
hypersensitivity test was performed on the day of artificial insemination
when cervical mucus and sera samples were also collected. The
animals were divided into groups according to the total number of
inseminations, number of inseminations per calving and their age. The
titer of antibodies was determined by the indirect immunofluorescence
method using sperm cells suspended in Tris egg-yolk extender.
Our results indicate that the intensity of CBHR is lowest in cows
inseminated once or twice and in younger animals, while, there were no
differences between the groups when they were formed according to
the number of inseminations per calving. Titers of antisperm antibodies
(ASA) of the Ig A class in cervical mucus (CM) increased with the
number of artificial inseminations, number of inseminations per calving
and age of the cows.