I have Windows server 2008R2 domain server in the network on which ERP application is working. I want to give remote desktop connection access / Terminal service to the users to access the server with the load balancing for approximately 40 users in the network.

I don't quite understand your question...what do you mean how can you load balance on a single server? It requires multiple servers to load balance. I am assuming you are wanting to load balance RDP connections correct? to do this you need to create a session host.

Dear Sir, I have one ERP application server to which the approximately 40 users connect through Remote Desktop Connection. My query is how to balance the load of this 40 users distributing them in groups of 20 + 20 users connecting to Application server. Kindly advice.

Yes you will need at least 2 servers and maybe a third to be the session host that does the load balancing (i don't recall if you can use one of the servers for this) but the link i sent you should give you plenty of information on how to set this up. but again you need more than one server.

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