TetR Monoclonal Antibody for Detection of Tet-On and Tet-Off Proteins

TetR Monoclonal Antibody (Clone 9G9) is a monoclonal antibody raised against the full-length wild-type bacterial Tet repressor protein (TetR), which is an essential component of the Tet-On and Tet-Off transactivator proteins.

The TetR antibody can be used for Western blotting and greatly simplifies the process of developing a Tet-inducible expression system by providing a method for early detection of the Tet-On 3G, Tet-On Advanced, and Tet-Off Advanced proteins.

The monoclonal antibody detects the following proteins on a Western blot*:

Tet-On 3G

Tet-On Advanced

Tet-Off Advanced

Tet Repressor (TetR)

Tet-Off

*This TetR antibody is not recommended for use with the original Tet-On or Tet-tTS proteins.

Note: Antibody-based detection of these tetracycline transactivators in cell lines is not a substitute for a functional test. Some highly inducible clones may express tetracycline transactivators at levels that are undetectable on a Western blot.

Applications

Source

Mouse hybridoma cells

Properties

Form: Affinity-purified monoclonal antibody in PBS

Isotype: IgG1 kappa

Concentration: 1 mg/ml

Suggested Dilution: 1:1000 for Western Blotting

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.