性能

形式

Liquid

存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

存储溶液

pH: 7.40Preservative: 0.02% Sodium azideConstituent: PBSNote: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.

应用

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用

Ab评论

说明

ICC/IF

Use a concentration of 5 µg/ml.

WB

Use a concentration of 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa).

靶标

功能

Prevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy.

组织特异性

Expressed in motor neurons, but not in sensory neurons. Found in liver and placenta, and to a lesser extent in spinal cord.

Predicted band size : 160 kDaObserved band size : 160 kDaAdditional bands at : 31 kDa,35 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 4 minutes

Immunocytochemistry/ Immunofluorescence - NAIP antibody (ab98020)

ICC/IF image of ab98020 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab98020, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HepG2 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa cells at 5µg/ml.