Dear Readers,
I have been trying to measure interleukin by using a thymidine uptake
(cell-proliferation) assay. I wonder if the specific activity of the thymidine
and the storage of tritiated thymidine has any effects on the uptake. What
is the consensus (if any) specific activity of thymidine one should use and
how long can one store tritiated thymidine and use it without any deleterious
effects?
Thanks a lot!!!
Sincerely,
Amrut Bhogle