What microbes cause ulcerative STIs?

What causes chancroid?

H. ducreyi

3

How do you diagnose chancroid?

1. No evidence of T. pallidum infection by DFM of exudate or DFA-TP2. Presentation of ulcers not typical for HSV or HSV culture negative3. Confirm by culturing on Fildes-enriched gonococcal media (blood, AA, vitamins, serum) in a 10% CO2 atmosphere at 33-35C.

4

Name three pathogenic spirochetes

TreponemaBorrelia (lyme disease)Leptospira (zoonotic diseases)

5

Which spirochetes can be seen on light-field microscopy?

Borrelia is the only one large enoughOthers must be visualized via dark field microscopy or with special stains