The second set of numbers next to the enemy's HP, POW, DEF, SPEED and Coins are stat increases from the Challenge Medal accessory; a 50% increase for HP, DEF, SPEED and Coins earned, and a 150% increase for POW.

Beehosses can typically unleash bees at Bowser to attack, and Bowser can hide in his shell to avoid them. However, if he goes in too early, the bee will notice and stop until Bowser comes back out. If the bee hits Bowser's shell and gets knocked out of the battle, Bowser can quickly punch the bee towards the Beehoss responsible for unleashing the bee. Mario and Luigi also fight Beehosses when they're able to get out of Bowser's body. They act nearly the same way they do with Bowser, except the bees tilt their stingers to the brother they're going to attack and slowly fly towards them, while Mario and Luigi have to jump over them. Beehosses have high HP so using attacks that involve fire are recommended. They are incredibly powerful, and can do a lot of damage if the Mario Bros. fail to time their attacks. Additionally, Bowser can still suck in their pollen, however, Mario and Luigi cannot.

Beehosses return as enemies in Mario & Luigi: Dream Team, now with spikes on their head, red eyes, and a darker color. In the field, they unleash a bee upon sighting Mario and Luigi, which chases them, stopping after going a certain distance away from the Beehoss who let it out. If the bee is touched, a battle with the Beehoss who let it out will start. The bee will disappear after a period of time.

In battle, they attack using a default group of nine bees, somewhat similar to their behavior in Mario & Luigi: Bowser's Inside Story. If a Beehoss runs out of bees due to them all being defeated, the Beehoss will run away. As Beehosses, unlike the previous game, count as spiked enemies this time around, they thus cannot be jumped on. Beehosses are weak to and thus take critical damage against Luigi's Fire FlowerBros. Attack.

After being attacked while it has bees in its hive, it will send them out towards the attacker, sometimes the group coming at Mario or Luigi at a harder to jump over upward angle that hits them if they just idle. If a Beehoss has no bees in its hive due to them not coming back after attacking, it may take a turn to have the bees return to its hive.

A Beehoss may attack by sending out a swarm at Mario or Luigi, which must be jumped over. If any bee is counter-attacked and defeated, the remaining bees will come back and attack the Bro. who counter-attacked the bee(s), which cannot be avoided. The bees will then either head into the background, forming an M or L formation several times. The fewer bees there are remaining, the less obvious the targeted Bro. is. The bees will fly back down into the midst of battle from the top-right corner, which must be avoided by jumping over them with perfect timing.

Beehosses can also send out two groups of bees at once, one in line with Mario, and one in line with Luigi (though in the case there is only one bee left, only one Bro. is targeted). One group of bees will land slightly before the other, that group will move at the Bro. it's in line with first, shortly followed by the other group, requiring the Bros. to jump when the group in front of them moves towards them. If either Mario Bro touches a bee, it may cause POW-Down.