Aph-1 is a component of the gamma-secretase multiprotein complex, which consists of presenilin (PS), nicastrin, Aph-1 and Pen-2, which are all necessary for full proteolytic activity. g-Secretase is the proteolytic activity responsible for the cleavage of a series of integral membrane proteins, most notably the Amyloid Precursor Protein (APP) and Notch, but also including ErbB-4, E-Cadherin, LRP, and CD44.1 Aph-1 and Pen-2 are the limiting cellular factors that control presenilin expression, and thus, g-Secretase’s catalytic activity. Because g-Secretase is responsible for the release of amyloid peptide from APP, current research has centered around using g-Secretase as a potential Alzheimer’s disease drug target.

Two mammalian homologues of Aph-1 have been identified: Aph-1a and Aph-1b, of which Aph-1a is more abundantly expressed than Aph-1b in human tissue samples. In addition, Aph-1a also occurs as two splice isoforms, long and short, that diverge at the C-terminus.3

Catalog #

A2298-77

Applications

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution

Western Blot: 1-3ug/ml

Immunoprecipitation: 7-10ug/IP reaction

Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Clone Type

Polyclonal

Isotype

IgG

Host

Rabbit

Source

Human

Concentration

~0.12mg/ml

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.

Purity

Purified by immunoaffinity chromatography.

Immunogen

Synthetic peptide derived from the C-terminal region of human Aph-1a.

Specificity

Recognizes human Aph-1a at ~29kD. A fragment of Aph-1a may also be observed at ~23kD. Recognizes only the long isoform of Aph-1a. Reactivity has been confirmed with human Aph-1a- transfected N2a mouse neuroblastoma lysates. Bands representing both endogenous and exogenous Aph-1a were observed. The non-transfected N2a cell lysates only showed endogenous Aph-1a expression. Species Crossreactivity: mouse.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.