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This product can be added to a Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.

This product can be added to a Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.

This product can be added to a Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.

This product can be added to a Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.

Cytotoxicity assays based on detection of biomarkers released into the media can underestimate cytotoxicity in long-term exposures of 72 hours or more because of limited stability of the biomarker detected. The CellTox™ Green Cytotoxicity Assay provides an easy, fast and accurate method to determine toxic effects during or after long-term exposure of cells in culture. CellTox Green can be combined with other methods in multiplex assays to determine mechanism of toxicity, and is easily scalable from 96- to 1536-well plate formats.

Perform Kinetic Cytotoxicity Measures to Determine Onset of Toxicity

Dose- and exposure-dependent increases in cytotoxicity

In the experiment shown here, bortezomib caused appreciable cytotoxicity in K562 cells between the 4- and 24-hour time points. Additive fluorescence at later time points indicates continued loss of membrane integrity occurring as a function of cytotoxicity against remaining cells. The CellTox™ Green Dye was added at seeding.

Multiplex for More Informative Data per Well

Measure Cytotoxicity and Viability

Here, CellTox™ Green Dye was mixed with K562 Cells, which were plated, then dosed with compound. CellTiter-Glo® Cell Viability Assay Reagent was added at the end of the 72-hour exposure and luminescence (viability) measured. These two inverse measures of cell health resulted in EC50 agreement.

Determine Appropriate Timing of Caspase Assays

CellTox™ Green can be used in kinetic mode to determine the onset of cytotoxicity followed by multiplexing with the Caspase-Glo® 3/7 Assay in the same sample well to confirm caspase-3/7 activation at the appropriate time.

Differentiate Between Cytotoxic and Cytostatic Effects

In this example, K562 cells were exposed to Methotrexate, an anti-proliferative compound, for 72 hours. CellTox™ Green Dye was added to determine cytotoxicity, then CellTiter-Glo® Reagent was added to the same sample well for viability determination. A viability assay alone would have misreported the compound as cytotoxic due to less cells in the treated sample compared to the non-treated control, which continued to proliferate. Multiplexing with a cytotoxicity assay reveals no change in signal as a result of compromised membrane integrity, indicating growth inhibition and not cytotoxicity.