The cue sheet you've made looks faulty. First, there are no postgaps or pregaps. Second, as long as your ISO and WAV files are in the same directory (which is how it should be set up), there is no need to use absolute paths. Third, avoid using long file names, esp. file names containing spaces. Lastly, there is no need to use the TITLE command, esp. when the title you're giving it is meaningless (i.e., "001_Unknown Artist - Track 2" ).

I suggest you rename the audio file as TRACK02.WAV and try this cue sheet: