Increased mitochondrial and peroxisome pathway activity in Cidea and Cidec double-deficient adipocytes. The BAT from newborn mice was used to isolate stromal vascular cells. The cells were then induced to differentiate to generate the data presented in A–E. The WAT from 1-month-old mice was used to isolate stromal vascular cells. The cells were then induced to differentiate to generate the data presented in F–J. A and F: Adipocytes were stained with BODIPY (boron-dipyrromethene) (white, indicates LDs), MitoTracker (red, indicates mitochondria), PMP70 (green, indicates peroxisomes), and Hoechst (blue, indicates nuclei). Scale bars, 10 μm. B, C, G, and H: OCR in the indicated adipocytes. Oligomycin, FCCP, and rotenone/antimycin A (RA) were added at the indicated time points. Cells from five mice were used. D and I: Catalase activity of the cells (n = 4). E and J: Western blot analysis showing the levels of various proteins in primary brown adipocytes. WT, wild type. *P < 0.05, **P < 0.01, and ***P < 0.001.