SALMONELLA SHIGELLA AGAR

Technical Data #1127a / 2013.11.01

The Salmonella Shigella Agar is a selective
medium for isolation of Salmonella and Shigella from stool and food
products. Brilliant green, bile salts, sodium thiosulfate and citrate are the selective
agents. A colony with black center confirm H2S production by thiosulfate
reduction.

FORMULAin grams per liter purified filtered water

Beef Extract

5,0

Casein Peptone

2,5

Meat Peptone

2,5

Lactose

10,0

Bile Salts

8,5

Sodium Citrate

8,5

Sodium Thiosulfate

8,5

Ferric Citrate

1,0

Neutral Red

0,025

Brilliant Green

0,00033

Agar

13,5

pH 7,0 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched
to obtain best results.

PRECAUTIONSThis medium is for IN VITRO diagnostic use only.

STORAGEStore prepared media at 2-8° C protected from direct light and dehydrated powder,
in a dry place, in tightly-sealed containers at 2-25° C.

SIGN OF DETERIORATIONMedia should not be used if the expiry date has passed. Prepared media should not
be used if there are signs of contamination or deterioration (shrinking, cracking,
evaporation or discoloration). Do not use dehydrated media if it is caked.

DIRECTIONSSuspend 60 g of dehydrated media in 1000 ml of purified filtered water. Heat with
frequent agitation and boil for one minute. DO NOT STERILIZE. Cool to 45-50°
C. Mix gently and dispense into sterile Petri dishes.

PROCEDUREPrior to inoculate, the prepared media should be brought to room temperature.

noculate plates with specimen and streak to obtain isolated
colonies.

Incubate at 35° C for 24 hours.

Inoculate also a selective broth for better isolation.

Examine for lactose non-fermenting colonies, with or without
H2S.

QUALITY CONTROLResults after 24 hrs at 35ºC

Organisms

ATCC

Growth

Salmonella typhimurium

14028

+ w/ H2S

Shigella flexneri

12022

+

Escherichia coli

25922

- or partial

Enterococcus faecalis

29212

- or partial

LIMITATIONS OF METHODIt is recommended to inoculate specimens into some selective broth as enrichment
to improve Salmonella and Shigella recovery. This medium is only a part of the identification.
Other tests may be required.