Figure 1.

Re-screening the candidate genes identified in previous Wg and Hh screens using the
DRSC-v dsRNAs. (a-c) Wg assay: 148 of 204 (73%) dsRNAs screened had reproducible effects on the Wg responsive
reporter activity (a); 58% of the DRSC1.0 dsRNAs that were predicted to have OTEs
repeated in the validation screen (b); while 84% of DRSC1.0 dsRNAs that were predicted
to have ≤5 OTs could be reproduced with DRSC-v dsRNAs (c). (d-f) Hh assay: 179 of 351 (51%) validation dsRNAs had reproducible effects on the Hh responsive
reporter activity (d); 24% of the DRSC1.0 dsRNAs that were predicted to have OTEs
repeated in the validation screen (e); while 64% of DRSC1.0 dsRNAs that were predicted
to have ≤5 OTs could be reproduced with DRSC-v dsRNAs (f).