1. Channel 1 + Channel 2

Segment objects using a multi or
any single channel image. In example below, 2-Channel Image was segmented using
the Blue channel. Cell #1 is highlighted in magenta and has an average grey
scale value of 144.47. A binary image (and cell numbering mask) is
automatically generated with the measurement and is saved by the menu command
REFERENCE > Binary -> Reference binary.

2. Channel 1 (Blue)

The cell numbering g binary mask is applied to the Blue
channel by selecting the blue channel and using the menu command REFERENCE >
Binary <- Reference binary. Note the average value for cell #1 is 191.19.

3. Channel 2 (Red)

The cell
numbering g binary mask is applied to the Red channel image by selecting the
image Red Channel image and using the menu command REFERENCE > Binary <-
Reference binary. Note the average value for cell #1 is about 97. Note that the
value 144 is the average of the values 191 and 97.

4. Background Measurement

Using a region
of interest ROI tool select an area of the background. In the example below,
the average background is 8.03.