The pSV40-GLuc Control Plasmid
is a mammalian expression vector that encodes
the secreted luciferase from the copepod Gaussia
princeps as a reporter, under the control of the constitutive
SV40 promoter. Gaussia luciferase (GLuc)
is a 19 kDa protein encoded by a “humanized “
sequence, and it contains a native signal peptide at
the N-terminus that allows it to be secreted from
mammalian cells into the cell culture medium (1) There is a multiple cloning site (MCS) upstream of
the SV40 promoter.

Features

Multiple samples can be obtained from the same
transfected cells (i.e., before and after experimental
treatments or at multiple time points).

90–95% of GLuc activity is found in the cell
culture medium, with the remaining 5–10%
detectable in cell lysates. This allows
flexibility when assaying GLuc along with other
co-transfected reporters.

The activity of GLuc is high and the GLuc assay
is sensitive enough to detect very small amounts
of GLuc enzyme activity.

GLuc is very stable in the cell culture medium so
the GLuc activity detected reflects the amount of
GLuc secreted by the transfected cells over a period
of several days. GLuc can also be stored at
4°C for several days without any loss in activity.

GLuc does not use the same substrate as
Cypridina luciferase. Therefore, it is possible
to assay both GLuc and CLuc independently in
cell culture medium from cells expressing both
reporters (2,3).

The pSV40-GLuc Control Plasmid can be transfected
into cells using any standard transfection and
stable cell lines can be established using Neomycin
selection.

Product Source

Advantages and Features

Applications

The pSV40-GLuc Control Plasmid can be used as
a control for assessing the efficiency of transfection
in mammalian cells. Plasmids containing other
constitutive promoter elements are also available
(see Companion Products Sold Separately).

GLuc can be used as a stand alone reporter or in
conjunction with other compatible reporters such
as Cypridina luciferase (CLuc) (2). GLuc and CLuc
are ideally suited for co-expression as both are
secreted and highly active enzymes providing ease
of use and sensitivity (2, 3).

Properties and Usage

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl1 mM EDTApH 7.5 @ 25°C

Licenses

By opening this package or by using the materials enclosed within;
Recipient is legally bound and accepts the following terms and
conditions:

Non-Commercial Entities: This product is covered by U.S. Patent No.
6,232,107 and other patents that are the legal property as assigned to
Prolume Ltd./Nanolight Technologies. This product is licensed only to the
purchasing laboratory-research group. Recipient agrees not to transfer
this plasmid or derivatives of this vector to any other laboratory, person
or research group, even if within the same institution.

Recipient agrees not to alter or make any changes to the nucleotide
coding sequence or secretory coding sequence of the luciferase(s)
contained within without prior written permission from Prolume Ltd./
Nanolight Technologies (www.nanolight.com). Recipient agrees not
to file for any patent rights or inventions claiming any portion of the
Luciferase(s) within this material without prior written permission from
Prolume Ltd./Nanolight Technologies.

Commercial For-Profit Entities & Non-Profit Foundations: (herein
referred to as Commercial Recipients): Commercial Recipients wishing
to derive products, engage in the sale or license of any products,
discover drugs, or make inventions by use of the materials enclosed, fully
agree to the terms mentioned above for Non-Commercial entities; AND
ADDITIONALLY agree to and are hereby bound to use the materials FOR
EVALUATION PURPOSES ONLY. Commercial Recipient hereby agrees
to destroy and cease use of any materials or derivatives containing any
portion of these materials within 180 days from receipt. Commercial
Recipient agrees not to use the materials for any use, other than the
180-suitability evaluation without prior written permission or obtaining a
valid license from Prolume Ltd./Nanolight Technologies.

Any Recipient that does not accept the license terms mentioned above,
shall return the unopened package and materials to NEB for a full refund.

Trademarks

BIOLUX® is a registered trademark of New England Biolabs, Inc.
QIAGEN® is a registered trademark of Qiagen.

Supporting Documents

The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.