An American Airlines flight attendant has been suspended after he was filmed challenging a passenger to fight, while a tearful mother of twins is chucked off a flight.

The confrontation took place when the airline's employee "violently" removed a stroller from the woman, hitting her with it and narrowly avoiding hitting one of her children, according to Facebook user Surain Adyanthaya who posted a video of the incident on the social media site yesterday.

The video has been shared over 5,000 times and follows in the wake of the United Airlines incident, when 69-year-old Asian doctor David Dao was dragged off a flight in Chicago with blood running down his face.

Mother of twins was 'violently' hit by stroller by flight attendant before ruckus began (Image: Facebook)

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The two then face up to each other in the aisle of the plane and the employee can be heard challenging the passenger to hit him.

The passenger eventually goes back to his seat and the flight attendant leaves the plane.

"We are deeply sorry for the pain we have caused this passenger and her family and to any other customers affected by the incident," the airline said in a statement late on Friday.

"The actions of our team member captured here do not appear to reflect patience or empathy, two values necessary for customer care. In short, we are disappointed by these actions. The American team member has been removed from duty while we immediately investigate this incident."

The woman and her family were being upgraded to first class for the remainder of their international trip, it said.