A 1:1 mixture of Leibovitz's L-15 medium and McCoy's 5a medium with 2 mM L-glutamine, 55%;
tryptose phosphate broth, 5%;
chicken serum, 20%;
fetal bovine serum, 20%
The fetal bovine serum concentration may be reduced to 10% after the cells have been in culture for 10 to 14 days.

Subculturing

Medium Renewal: Every 2 to 3 days

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10 exp5 viable cells/ml.

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.