Pipettors, pipet tips and other common lab consumablesOrbital shaker or oscillating rockerTissue Paper, blotting paper or chromatography paperAdhesive tape or Saran WrapDistilled or de-ionized waterA chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

使用细节

使用细节

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应用备注

Perform ALL incubation and wash steps under gentle rotation or rocking motion (~0.5 to 1 cycle/sec) using an orbital shaker or oscillating rocker to ensure complete and even reagent/sample coverage. Rocking/rotating too vigorously may cause foaming or bubbles to appear on the membrane surface which, should be avoided. All washes and incubations should be performed in the Incubation Tray (ITEM 10) provided in the kit. Cover the Incubation Tray with the lid provided during all incubation steps to avoid evaporation and outside debris contamination. Ensure the membranes are completely covered with sufficient sample or reagent volume during each incubation. Avoid forceful pipetting directly onto the membrane, instead, gently pipette samples and reagents into a corner of each well. Aspirate samples and reagents completely after each step by suctioning off excess liquid with a pipette. Tilting the tray so the liquid moves to a corner and then pipetting is an effective method. Optional overnight incubations may be performed for the following step to increase overall spot signal intensities:- Sample Incubation- Biotinylated Antibody Cocktail Incubation- HRP-Streptavidin Incubation

说明

The C-Series arrays feature chemiluminescent signal detection. The antibodies are spotted on nitrocellulose membrane solid supports and are handled in a very similar manner to Western blots. All C-Series arrays work on the sandwich ELISA principle, utilizing a matched pair of antibodies: an immobilized capture antibody and a corresponding biotinylated detection antibody.

样本量

1 mL

板类型

Membrane

实验流程

Block membranes

Incubate with Sample

Incubate with Biotinylated Detection Antibody Cocktail

Incubate with HRP-Conjugated Streptavidin

Incubate with Detection Buffers

Image with chemiluminescent imaging system

Perform densitometry and analysis

限制

仅限研究用

贮存及处理

贮存及处理

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注意事项

The antibody printed side of each membrane is marked by a dash (-) or number (#) in the upper left corner. Do not allow membranes to dry out during the experiment or they may become fragile and break OR high and/or uneven background may occur. Grasp membranes by the corners or edges only using forceps. DO NOT touch printed antibody spots.

储存条件

-20 °C

储存方法

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes a -20°C and all other reagents undiluted at 4°C for no more than 3 months.