In 1988 the tolerance of corn, sorghum, sorghum-sudangrass. and pearl millet hybrids to simazine and alachlor was tested in field during the growing season and pots during the summer and fall. In field and summer pot experiments(Exp.) the above mentioned four crops were tested at the ratios of simazine WP (50% ai, g/10a) ; alachlor EC (43.7% ai. ml/10a) of 130: 0, 100: 0, 70: 200, 0: 300 and 0: 400 and a sorghum hybrid was tested at 0, 50, 100, 200, 300, and 400ml/10a of alachlor and 70g/10a of simazine+ 200ml/10a of alachlor in fall pot Exp. In corn emergence rate, percent stand, plant height of seedlings, and dry matter(DM) yield were not affected by simazine and alachlor in all Exps. In sorghum and sorghum-sudangrass early growth and DM yield were not affected by simazine and alachlor in field Exp. In contrast, simazine reduced height and dry weight of seedlings slightly without any deterimental effects on emergence and survival rates. but alachlor reduced survival rate, plant height, and dry weight of seedlings significantly in summer pot Exp. In fall Exp. alachlor did not affect emergence rate of a sorghum hybrid, but survival rate, plant height, and dry weight of seedlings reduced with increased levels of alachlor when applied higher than 100ml/10a. In pearl millet simazine did not affect emergence rate, plant height, and DM yield in field, but reduced survival rate, plant height, and dry weight of seedlings in summer pot Exp. However, alachlor reduced DM yield significantly due to a lower percent stand even in the field. In summer pot Exp. although emeregence rate was slightly reduced, all seedlings were dead after emergence. Simazine did not control grasses such as Digitaria sanguinalis, Setaria viridis, Echinochloa crusgalli effectively, but controlled broadleaf weeds. Alachlor controlled all grasses, Porluraca oleracea, and Amaranthus mangostanus, but did not control Acalypha australis and Chenopodium album. A combination of simazine and alachlor controlled weeds more effectively than either simazine or alachlor alone.

This experiment was carried out to investigate the lectin of soybean (Glycine max L.) seed. Purification was done by 50-80% ammonium sulfate precipitation, CM-cellulose and Sephadex G-100 column. The purity was ascertained by electrophoresis. The molecular weight of purified lectin was estimated as 132,000. It was composed of three subunits which molecular weight was 45,000. The lectin was identified as glycoprotein by Schiff's reagent staining and Dubois method. The lectin agglutinated erythrocytes of rabbit and human. The amounts of the lectin to agglutinate human erythrocytes differed among the blood types: The blood type A required the least amount, the next was B, O, and AB in order. The agglutination was specifically inhibited by 5g/ml of N -acetyl.-D-galactoseamine and 200g/ml of D-galactose. Other tested sugars could not inhibit the agglutination of the erythrocytes by the lectin.

An experiment was conducted at the Yeongnam Crop Experiment Station to obtain basic informations about cultural techniques for high yield by manipulating plant spacing in association with seedling number. Cultivars showing relatively low competition index within or between rice hill were Palgongbyeo, Milyang 83. Milyang 90 and Sangpungbyeo, while those with high competition index were Pungsanbyeo, Samgangbyeo, Weongpungbyeo, Taebaegbyeo and Dongjinbyeo. Variations of agronomic characters such as culm length, spikelets per panicle, grain yield per panicle among each culm within hill were not interrelated among the characters. This phenomenon was also same within and between cultivar groups. However, cultivars having high competitive ability showed relatively low variation in agronomic characters. There was a trend that most of rice cultivars had a high positive correlation between total competition index and rice grain yield. Competition index between rice hill was more significant than that of within rice hill for Tongil type cultivars except Milyang 83 which belonged to low tillering capacity while both types of competition index within and between hill were important for Japonica type cultivars and Milyang 83 of Tongil type cultivar to increase rice yield.

In order to estimate field seedling emergence and grain yield by the information collected from various laboratory seed vigor tests, each two malting and naked barley cultivar seeds were artificially aged according to the standard aging treatment suggested by the Association of Official Seed Analysts. The seed vigor tests adopted were warm- and cold-germination test, tetrazolium vigor test and electrocon. ductivity test. Field emergence of malting barley (Y) was estimated by Y=-2.962+0.229X (% warm germination) -0.001X (vigor of warm germination test: WGT) +0.354X (vigor of cold germination test: CT) -0.558X (% cold germination). The multiple correlation coefficient indicated that % warm germination. was contributed 64 % of the variation in seedling emergence rate of malting barley. The vigor of warm and cold germination tests, and % cold germination contributed additional 4, 7, and 9%, respectively, upon addition of the variables into regression. For naked barley, the regression equation of emergence rate was less efficient(R=54%) than that of malting barley(R=84%). A model to predict grain yield by the results of various seed vigor tests was not evaluated for both malting and naked barlev.

Anther and/or tissue culture of cross pollinated crops would be very important because it can result in the direct use of haploids or doubled haploids for developing superior hybrids or varieties. The objective of the study was to investigate the response frequencies in anther and/or tissue-cultured hybrids of corn. pearl millet and buckwheat to identify agronomically acceptable germplasm of the crops. 27 crosses of corn inbred lines were evaluated by plating their anthers on N6. MS and Yu-Pei media. Two genotypes of FR1l41/FR16 hybrid cultured on N6 medium and Fla 2BT73/S6013 hybrid cultured on N6 medium responded with one anther producing calli when plated after 5 low temperature treatment for one week. Immature embryos of corn hybrid Suwon 19 responded producing calli that were regenerated to plants at a 8.6 percent success rate. Of the 20 corn hybrids. immature tessels of FR1l41/FR16. B68/A1l6N//KS15. KS16/KS17. GA209/DB578 and SDB126/GA209 crosses responded at a relatively higher success rate producing calli that were regenerated to plants. In tissue culture of elongating culms of pearl millet x Napier grass interspecific hybrid. 2.5-4.0mm long pieces of the culm were good for callus induction resulting in higher success rate. The epicotyl of buckwheat was very good for tissue culture. and the node produced the plants regenerated directly without callus induction on the B5 medium containing I ppm BA and 0.05 ppm IBA. There were great differences in response to anther and/or tissue culture of corn, pearl millet and buckwheat due to genotype x medium and environment interactions.

The experiments were carried out to compare the growth characteristics of excised seminal roots in Japonica type, Indica- Japonica hybrids and its parents. Based on the modified culture medium by Kawata et al., several levels of pH. sucrose and casamino acid were varied in the culture media. The excised root length at 27 incubator was little different between Japonica type and Indica-Japonica hybrids, but at 30 incubator the excised root elongation of Indica-Japonica hybrids was much better than that of Japonica type. The dry matter weight of excised root in Indica- Japonica hybrids was significantly heavier than in Japonica type at both 27 and 30 incubators. The difference of dry root weight between Japonica type and Indica- Japonica hybrids was caused from the number of lateral roots, especially the root numbers of 25 cm length. The main and lateral seminal root growth of Japonica type grew best at the lot of 0.2 percent casamino acid. However, the lots of 0 and 0.2 percent casamino acid showed good results in the main and lateral excised root growth. respectively of Indica-Japonica hydrids. The growth of excised roots was better in Indica-Japonica hybrids than in Japonica type at even various levels of pH, sucrose and casamino acid. Between the parents of Indica - Japonica hybrids, the excised root growth of Indica type was more predominant than that of Japonica type, and the effect of casamino acid on the excised root growth in the formar was similar to that of Indica type. This result indicated that the growth characteristics of excised seminal roots in Indica Japonica hybrids were derived from the parent, Indica type.

This experiment was conducted to investigate the endosperm characteristics of an opaque rice which was derived from an Indica cultivar Pokhareli Mashino. Grain apperance of opaque rice looked similar to the waxy grain, while the blue-color response to the iodine solution was similar to non-waxy endosperm. The shape of starch granules was alike each other, but their size were different each other with the smallest of the opaque rice. Their cristaline structure of starch granules were A type. The content of total protein and most of the amino-acids of opaque rice was about 1.5 times of other varieties. Alkali digestibility of the opaque rice was very low. Total water uptake and hydration rate of opaque rice was similar to non-waxy variety Tongil. Amylogram characteristics such as viscosity, setback and consistency of opaque rice revealed medium value between. waxy and non-waxy rice.

Seasonal climatic factors associated with tobacco quality grade and production rate were analyzed. The degree of influence on yield distribution rate in high guality tobacco leaues was highly positive with the average temperature in early May, but negatively related to those in late May and early June. Positive correlations were noticed between the degree of influence and sunshine hours in Middle June, late June and late May in decrease order, while negative degree of influence was higher in early May than in late May, The order influenced by recipitation in a positive direction was early May, late May and middle May. Negative influence was noticed in middle and early June with a great degree.

Lower leaves of aromatic tobacco are also much lower in Quality than upper leaves. So feasibility test of no harvesting and curing of lower leaves was conducted under high planting density and high nitrogen conditions with conventional cultural system. Effect of harvesting time on yield and Quality were investigated under 2 nitrogen levels. Among harvesting methods of conventional harvest with priming under high planting density, no-harvest of first priming, removal of lower leaves which relevant to first prime stalk before maturity, no-harvest of first and second priming. no-harvesting or pruning of first prime stalk before maturity was best in yield, price and in crude income. The shortor the harvest period became, the lower the yield, price and contents of reducing sugar and nicotine became, but reverse in this trends with total nitrogen and protein nitrogen. So 6 or 8 days interval of harvest is most recommendable.

The effect of various supplementary light sources on the agronomic characteristics has been investigeted for generation acceleration of barley in green house with the condition of 15 and 8 hrs. day light followed by each supplementary light sources including incandescent electric lamp (IN), Blue fluorescent lamp(BF). day-light fluorescent lamp(DF), Biolux lamp(BL), BL+BF, In+BF and IN+BL. Leaf number of main stem, leaf area, culm length, dry matter yield of shoot, number of grain per spike, grain yield per plant were most sharply decreased with the exposition of IN, while flag-leaf emergence on main stem and I, 000 grain weight were earlist and heavist respectively with the exposition of IN. In appeared to be best light source for shortening the days to heading, but IN + BF will be most desirable light source with considering both days to heading and adequate yield at the same time.

Two sweet corn hybrids, 'Tanok l' and 'Golden Cross Bantam 70 (GCB 70)' were grown at five plant densities, of 4, 167. 5,556, 6,667. 8, 333, and 11, 111 plants per 10 ares, with or without tiller removal, to determine effects of tiller removal on growth and yield of sweet corn hybrids at various plant densities. Tillers were pulled when less than 15 cm tall. The number of tillers per plant linearly decreased as plant density increased. The two hybrids had similar plant height, ear length and diameter, ear weight and the number of ears per plant and 10 ares. Tanok 1 lodged approximately 20％ at above 8,333 plants per 10 ares, while GCB 70 did not lodge at all, at any plant density. Tanok 1 had higher leaf area index (LAI), ear and stover yields than GCB 70. Except for root lodging and LAI, hybrid x plant density interaction was not significant at 5％ probability level. Plant density did not affect silking data. Increasing plant density linearly increased plant height, LAI, and stover yield, but linearly decreased ear length, ear weight, and the number of ears per plant. Increase in LAI was greater in Tanok 1 than in GCB 70, with increasing plant density. The relationships between the number of ears and ear yield per 10 ares and plant density were Quadratic. The optimum plant density was estimated to be approximately 6500 plants per 10 ares, using the equation based on ear yield. Except for ear height and LA I, hybrid x tiller removal and plant density x tiller removal interactions were not significant. Hybrid x plant density x tiller removal interaction was not significant for any characters. When averaged over hybrids and plant densities, tiller removal reduced plant height and ear and stover yields by about 3, 10, and 16％, respectively, but did not significantly affect silking date, root lodging, ear length and diameter and the number of ears per plant and per 10 ares. The results indicate that the optimum plant density is approximately 6500 plants per 10 ares, regardless of tiller removal and tillers are not to be removed at any plant density.

Field experiment was conducted to find out the effect of lime materials application on yield, and chemical composition of Burley 21 in 1986. Lime materials and application rate were CaSO : Ca 35kg/l0a. CaCO : Ca 35kg/l0a and Liming: pH6.5. Contents of Ca and Mg in fresh or cured leaves were increased, but AI, Fe and Mn were decreased by applying lime materials. Yield were increased by applying lime materials. Among lime materials treatment, yield of limed and CaCO plot were higher than that of CaSO plot. Value per kg of cured leaves was not affected by applying lime materials. Cured leaves of CaSO plot contained higher NH-nitrogen and alkalinity number of water insoluble ash than those of unlimed plot. Cured leaves of CaCO, plot contained higher alkalinity number of water soluble ash, insoluble ash and volatile neutral constituent, but lower protein-nitrogen and petroleum ether extracts than those of unlimed plot. Cured leaves of limed plot contained higher alkalinity number of water soluble and insoluble ash, NO-nitrogen and volatile neutral constituents, but lower protein -nitrogen, nicotine and petroleum ether extracts than those unlimed plot. Yield was increased, however leaf Quality in respect to chemical and organoleptic characteristics were not affected considerably by applying lime materials. Therefore, it suggests that controlling the soil pH about 6.5 by liming might be necessary for tobacco cultivation.

Normally cultured, harvested and yellowed leaves of aromatic tobacco variety. KA 101 were sun-cured under conventional polyethylene film house, air-cured in burley curing barn, and bulk-cured at three temperatures of 40, 50 and 60. respectively, to investigate the effects of curing conditions on quality and chemical properties of leaf tobacco. Air-cured leaves had the poorest quality by price with little difference between that of sun-cured and three bulk-cured ones. Air-cured leaves were higher in ammonia nitrogen, protein nitrogen, total nitrogen, ash content and pH, but lower in sugar content when it compared with those of sun-cured and three bulk-cured ones. The curing variables tested did not affect the content of pet. ether ext. and volatile organic acids. The content of pet. ether ext., volatile organic acids, and most of volatile neutrals tended to decrease or not to change as the bulk-curing temperature increased. A quality index, (volatile organic acids, mg/l00g+％ pet. ether ext.) / ％(ash+pH) x 10, was found to be most accurate and easy to apply for the quality evaluation of aromatic tobaccos.

Tobacco and ginseng plants differed in responses to varied light intensities. Tobacco showed high in CO uptake and RuBPCase activity at 1900 E m/sup-2/ sec-1/, being high by 3.7 times and 2.7 times than ginseng respectively. Close positive relationships existed between CO uptake and RuBPCase activity in tobacco. However, ginseng showed negative correlation. The activity of glycolate oxidase and malate dehydrogenase in tobacco was high at 1900 E m/sup-2/ sec-1/, but those of ginseng was high at 1000 E m/sup-2/ sec-1/. Nitrate reductase activity of tobacco at 1900 E m/sup-2/ sec-1/ was 2 times higher than that at 500 E m/sup-2/ sec-1/, while that of ginseng was no detected in all plots. The content of protein and chlorophyll in tobacco was 2.2 times and 1.5 times higher than in ginseng at the most efficient light intensity. The ratio of chlorophyll a/b in tobacco was low at 500 E m/sup-2/ sec-1/, while that of ginseng was low at 1000 E m/sup-2/ sec-1/. The relationships between protein and chlorophyll was high positive correlation. However, on 5 days after treatment, ginseng showed negative correlation at 500 E m/sup-2/ sec-1/. Tobacco and ginseng showed different leaf soluble protein patterns on SDS-gel electrophoresis. The molecular weights of two major band were 50 KD and 15 KD in both plants. The major bands in tobacco were thinned at 500 E m/sup-2/ sec-1/, while those in ginseng thinned at 1000 E m/sup-2/ sec-1/ from 15days after treatment. Disappeared band was 45 KD at 500 E m/sup-2/ sec-1/ in tobacco, but that of ginseng was 47 KD at 1000 E m/sup-2/ sec-1/.