Two nutrient solution greenhouse experiments were conducted to identify morphological parameters that express Al tolerance and to determine the influence of period of plant growth and Al concentration necessary for tolerance discrimination, in two rice cultivars. Plants of known maximum root length were transferred to nutrient solution at four levels of Al (0, 80, 160 and 320 mmol L-1) with a pH 4.0. In each collection, the new maximum root length was measured in addition to root and leaf areas, and root and shoot dry weights. Only the morphological parameters related to the root system differentiated Al tolerance of the cultivars, and relative root elongation was the most sensitive. Four days of exposure to Al were sufficient to detect differences in Al tolerance by means of relative root elongation. The methods established in this study can be useful in evaluating larger number of cultivars.