Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation

Kidney from aborted ovine fetus

Product Format

frozen

Storage Conditions

Vapor phase of liquid nitrogen

Images

Comments

Please see ATCC Virology Guide for more detailed information on propagation

Effect on Host

Intracellular inclusion bodies visualized by fluorescent staining

Recommended Host

McCoy (ATCC® CRL-1696™)

Growth Conditions

Temperature: 37°CRecommendations for Infection: For best results, infection should be performed on an 80-100% confluent, 24-48 hour old cellular monolayer. Sonicate seed material for 20 seconds at approximately 240W to disrupt cells. Prepare dilution of chlamydia in minimum amount of volume (e.g. 1 mL per 25 cm2). Remove cell growth medium and inoculate with disrupted material. For adsorption, centrifuge at 1500 x g at 25°C for 1 hour. End adsorption by adding agent growth medium.Incubation: Incubate infected culture for 72 hours at 37°C in a humidified 5% CO2 atmosphere.Preservation: Controlled-rate freezing.

Everett KD, et al. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int. J. Syst. Bacteriol. 49: 415-440, 1999. PubMed: 10319462

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.