Thermostable Thermus Filiformis (Tfi) DNA Ligase

Tfi DNA Ligase plays role of formatting phosphodiester bond by connecting double stranded DNA or connecting oligonucleotides 3’-hydroxyl end and 5’-phosphate end together.
Especially the reaction temperatures are between 45°C and 65°C compared to different DNA Ligase such as T4 DNA Ligase, E.coli DNA Ligase, etc.
It is the higher temperature that keeps safe and active. In addition, Tfi DNA Ligase is possible to use in ligation reaction in high reaction temperature requirement.