Press the "Vertical Import" button if you want to import your date in a vertical manner (A1, B1, C1, etc.) or "Horizontal Import" in case you prefer a horizontal arrangement of your data on the plate (A1, A2, A3, etc.)

Define corresponding primer sources in column C. Please use only one of the following terms: "Premixed", "Standard", "Enclosed", "Stored", "Custom", "Order", "Design".

Step 5:

Copy/paste sample and primer names into the foreseen window

Step 6:

Press the "Vertical Import" button if you want to import your date in a vertical manner (A1, B1, C1, etc.) or "Horizontal Import" in case you prefer a horizontal arrangement of your data on the plate (A1, A2, A3, etc.)

Technical Datasheet and Different Types of Certificate of Analysis (CoA)

On our technical datasheet, you find information regarding your ordered oligos like oligo name,
oligo ID, nucleotide sequence, modifications, length, synthesis scale, molecular weight, melting
point, the amount in OD, nmol and µg and the information with how much water you need to dissolve
the dried oligo to obtain a 100 µM solution.

The technical datasheet does not meet your requirements?
For an extra charge, we do offer different types of CoAs for a higher level of documentation.
Our standard CoA (Type 1) contains the following information:

Appearance test by means of visual inspection

Solubility test by means of solubilization in buffer prior to lyophilization

Information about re-suspension of your oligo and the stability (shelf life at different storage conditions)

Identity test by means of MALDI-TOF including the chromatogram

Purity test by means of analytical HPLC including the chromatogram (if secondary structure is too strong, we do not perform this analysis)

Purity test by means of analytical PAGE

Proof checked and signed by our head of oligonucleotide department or the delegated representative

Further to the standard CoA are the following types of CoA's available:

Type

Description

1

Certificate of Analysis (complete)

2

CoA without declaration of oligonucleotide stability

3

CoA without Maldi TOF

4

CoA without analytical HPLC

5

only Maldi TOF spectra

6

only HPLC

You need a customized CoA? Please get in contact with us.

How to Proceed:

Step 1:

Use a standard Excel sheet

Step 2:

Define oligo names in column A (≤ 25 characters)

Step 3:

Define corresponding oligo sequences in column B

Step 4:

Click on "Copy/paste"

Step 5:

Copy/paste the oligo data from Excel into the foreseen window and press OK

Example

Important Remark
Don’t use : ; , " \ / * ? %3C %3E | # Tabulator.

Premixed Primer:
Primer added by customer.

Standard Primer List:
Primer added by Microsynth. Select your sequencing primer from a list of 94 standard primers which are stored at Microsynth (primarily for plasmids).

Stored at Microsynth:
Primer added by Microsynth. Prerequisite for this option: You have already sent the primer to Microsynth in the past. Your primer will be stored for 6 months at Microsynth.
The primer name will be automatically deleted from this list after 6 months or if the primer has been used up.

Custom Primer List:
Primer added by Microsynth. This option is an add-on to the option "Stored at Microsynth".
If you know that a primer will be used frequently in future orders, send us a larger volume of this primer (at least 50μl) and add it to your custom primer list.
We will then store it for an extended period (up to one year).

Order Now:
Primer added by Microsynth. Via this option you can order a new primer already during the order process without changing the order form.
While your DNA is on the way to Microsynth, we will produce your sequencing primer and forward it to our sequencing lab.

Design at Microsynth:
Primer added by Microsynth. This option is similar to "Order Now".
In addition to primer synthesis Microsynth performs also the primer design.
You just have to provide us by email with a sequence of 50 - 200 bases.

Please choose this option in case you want Microsynth to purify your PCR product (removal of PCR primers and dNTPs). Please note that this service is only available at an additional charge.

Please mark this checkbox only if you know that your sample contains GC-rich sequences or hairpins.

Please choose this option only in case you have difficult-to-sequence samples which have not been successfully sequenced by using our standard sequencing protocols (e.g. GC-rich Sequence).

Please note that the “Advanced Treatment” protocol is subject to following limitations/conditions:
- all samples of an individual order have to be marked with this specific sequencing protocol
- is not compatible with overnight sequencing
- is only available at an additional charge

Please contact us for additional information.

If you work primarily with specific sequencing primers which you will use again and again over the coming months, Microsynth recommends you to make use of the “Custom Primer List”.

How does it work?
- If you have choosen the primer source „Order Now“ or „Enclosed in Separate Tube“, simply mark this box and the specific primer(s) of this order will be added to your „Custom Primer List“
- Or alternatively go to the “Options & Preferences” section and select “Review/Edit Custom Primer List” under “Primer Management”. Here you can add and delete specific sequencing primers.

Your overall benefits:
- Your specific primers will be stored at Microsynth
- When placing your next order, you can easily select the requested primer(s) from your “Custom Primer List” by one simple click.
- When working in a larger lab, you will have the possibility to share specific sequencing primers amongst your research group. For this purpose, however, you have to first create and define your group. This can be done within the “Options & Preferences” section when selecting “Create Group/Show Group Members” under “Group Management”.

Our Special Sequencing Protocols are especially useful if you want to decode difficult-to-sequence constructs such as regions containing hairpin structures, G/C-rich sequences, etc.
Continuous optimization of our Sanger sequencing capabilities has resulted in protocols that are able to map difficult or even very difficult DNA templates with a success rate >98%.
Generally, we recommend the following two-stage approach to our customers:

Special Treatment Service – Stage 1: for difficult-to-sequence samples (resolves up to 95% of difficult DNA templates)

Special Treatment Service – Stage 2: for very difficult-to-sequence samples (resolves over 98% of difficult DNA templates)

With this pre-test, a few samples of your 96-well plate are picked out and sequenced. If the pre-test shows that quality/concentration of your DNA template and/or your primer are in the appropriate range, your entire plate will be sequenced. This initial test phase is free of charge but will delay delivery of your sequencing results by one working day. Microsynth generally recommends this pre-test unless you know in advance that requirements are given for yielding good sequencing results.

Please enter only last name of group leader (no first name or title is required)

If this field is not applicable for you, please mark the box „n.a.“

If you fill out a second email address, the confirmation of an oligonucleotide order will not only be sent to the main email address, but to the second address as well.

The registration of your sample stickers has the following advantages:

When filling the sequencing order form prior to shipping samples to Microsynth, you will easily see the range of available label numbers (valid labels that you have yet not utilized). You then simply select the required label numbers via a pull down menu (allows you to avoid the laborious and error-prone process of typing in the barcode label numbers in a manual manner).

You still can share labels amongst your group members (just use the tool “Group Management” under “Options and Preferences”) and have the overview about all valid barcode labels.

The registration of your barcode labels has the following advantages:

When filling the sequencing order form prior to shipping samples to Microsynth, you will easily see the range of available label numbers (valid labels that you have yet not utilized). You then simply select the required label numbers via a pull down menu (allows you to avoid the laborious and error-prone process of typing in the barcode label numbers in a manual manner).

You still can share labels amongst your group members (just use the tool “Group Management” under “Options and Preferences”) and have the overview about all valid barcode labels.

Registration of your Easy Run barcode labels:
Easy Run labels will be registered automatically to your Microsynth webshop account, when they are shipped.

Please remember to unregister the labels in the Options and Preferences
section, if you are forwarding labels to someone else. Otherwise the recipients will not be able to register the labels to their account -
and they will not be able to download their sequencing results from the webshop.

Overview about Your Custom Primer List

Name

Sequence

Recieved At

Expires At

Important Remark

This pop up window gives you an overview about your current custom primer list. If you want to modify your custom primer list, please go to the “Options & Preferences” section of our webshop and select “Review/Edit Custom Primer List” under “Primer Management”.

The primer is not stored at Microsynth.
Possible reasons:

The spelling of the primer name is wrong.

The expiry date has exceeded and the primer was discarded.

The primer has never been stored at Microsynth. Please check if you entered the correct primer source.

The primer name is not yet saved in our sequencing data base because you plan to order the primer in the oligo webshop after this sequencing order.
In this case, please choose the primer source “Design at Microsynth” or "Order Now".

cannot be offered within a punchout session,
because the final price calculation depends on the samples you are turning in.
To order this service, you need to open the Microsynth webshop directly at the link below.
This will end the current punchout session:
https://srvweb.microsynth.ch
or call us at:
+41 71 722 8333

Microsynth Oligonucleotides
Please be aware that Microsynth’s oligonucleotides are not sold through CARLO ERBA Reagents S.r.l.
Oligonucleotide orders will be invoiced through Microsynth to the billing address in your customer data.