Rabbit anti-Hemagglutinin (NT) protein polyclonal antibody was raised against a synthetic peptide corresponding to 15 amino acids at the amino terminus of the Hemagglutinin protein (Genbank accession no. AAT76166). Efforts were made to use relatively conserved regions of the viral sequence as the antigen. The antibody only recognizes the cleaved subunit not the full-length H5.

Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

ELISA

Use a concentration of 1 µg/ml.

Target

Relevance

Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. HA interacts with cell surface proteins containing oligosaccharides with terminal sialyl residues. Virus isolated from a human infected with the H5N1 strain in 1997 could bind to oligosaccharides from human as well as avian sources, indicating its species-jumping ability.

I can confirm that ab21292 only recognizes the full-length H5 and ab21297 will recognize a cleaved subunit.
Unfortunately, I cannot disclose the exact peptide sequence.
I hope this information will help you,

Thank you for your message and your valuable feedback which has now been passed on to me in the Technical Support department. I am very sorry to hear you have had problems obtaining satisfactory results using these antibodies. Often it is possible to m...

Thank you for your enquiry.
I have been in touch with the source of this antibody and I can tell you that unfortunately the immunogen sequence is proprietary. However, I can tell you that in their opinion the antibody should not cross-react to other...

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Unfortunately, the originator of the antibody will not disclose this information. The most specific information I can give you is what is listed on our datasheet, that the immunogen was selected from between residues 100...

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I have performed an alignment of the immunogen sequence with accession numbers AAW80717, AAT39066, and AAA43083.
I can tell you that the immunogen was selected from a conserved region of the sequence and shows the fo...

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Unfortunately I cannot provide you with details of the accession number that was used for the design of the immunogen for this antibody. However, I can perform an alignment of your H5 amino acid sequence with the immunog...

Thank you for your enquiry.
Unfortunately, the immunogen sequences for ab21292, ab21297, ab21304 and ab21305 are proprietary. However, I can give you a range from which we selected the peptides.
ab21305 - residues 150-200
ab21304 – residues 4...

Thank you for your enquiry.
I can not provide you with the exact sequence used, but it is 14 amino acids within residues 100-150 for ab21297 and within residues 300-350 for catalog number ab21292.
Please let me know if you have any more questions.

Thank you for your enquiry.
Unfortunately I do not have details of any cross-reactivity by this antiserum against non-H5N1 hemagglutinin proteins.
I have blasted the peptide sequence of the 15 N-terminal residues: MERIVIALAIISIVK derived from t...

Thank you for your enquiry. Ab21297 was generated using a synthetic peptide corresponding to 15 amino acids at the N-terminus of the Hemagglutinin protein. The exact sequence is proprietary. The antibody's specifity was tested by peptide ELISA in which...