The Republican National Committee uploaded a YouTube video labeling Biden's laugh a "cackle."

Mentions of Biden's laughter garnered the most tweets for a single term - more than 500,000 mentions, according to Signal at Yahoo.

Though Republicans sought to portray the attention on Biden's laughter as negative, social media analysis shows that the commentary - at least online - was split roughly evenly between positive and negative comments.

Even Biden's teeth became part of the action. A new meme emerged admiring "Joe Biden's white, pearly teeth."

One Twitter user, @smotus, commented: "Biden's laughs would be more of a problem if he didn't have such awesome teeth. #vpdebate"

Despite the focus on Biden's laughter, no single moment in this vice presidential debate rivaled the Twitter hysteria that was sparked by Big Bird during last week's presidential debate, which got 170,000 tweets per minuted.

But when Biden quipped, "Now you're Jack Kennedy?" Twitter activity spiked to its highest level of the night with more than 7,000 tweets per minute.

Overall, Twitter reported more than 3.5 million tweets for the vice presidential debate while the first presidential debate last week almost broke Twitter with 10.3 million tweets.