This study will evaluate and compare the effect of the amount of toothpaste used and brushing time on enamel strengthening (percent of surface microhardness recovery, % SMHR) and enamel fluoride uptake (EFU).

Change from baseline in the recovery of percentage surface micro hardness (% SMH), of brushing for 2 minutes versus (vs.) brushing for 45 seconds with 1.5 grams (g) of experimental dentifrice. [ Time Frame: Baseline and at 14 days ] [ Designated as safety issue: No ]

Percent SMH recovery (%SMHR) was calculated from hardness values of enamel specimens at baseline (B), after intra-oral exposure (R) and after in vitro demineralization (D1) using formula: [(D1-R)/ (D1-B)]*100. A greater percentage change in SMHR represents a better remineralisation and hence a better outcome.

Secondary Outcome Measures:

Change from baseline in the recovery of % SMH, of brushing for 2 minutes vs. brushing for 45 seconds with 0.5 g of experimental dentifrice. [ Time Frame: Baseline and at 14 days ] [ Designated as safety issue: No ]

Percent SMH recovery (%SMHR) was calculated from hardness values of enamel specimens at baseline (B), after intra-oral exposure (R) and after in vitro demineralization (D1) using formula: [(D1-R)/ (D1-B)]*100. A greater percentage change in SMHR represents a better remineralisation and hence a better outcome.

Change from baseline in the recovery of % SMH, of brushing for 2 minutes with 0.5g of experimental dentifrice vs. brushing for 2 minutes with 1.5 g of experimental dentifrice. [ Time Frame: Baseline and at 14 days ] [ Designated as safety issue: No ]

Percent SMH recovery (%SMHR) was calculated from hardness values of enamel specimens at baseline (B), after intra-oral exposure (R) and after in vitro demineralization (D1) using formula: [(D1-R)/ (D1-B)]*100. A greater percentage change in SMHR represents a better remineralisation and hence a better outcome.

Change from baseline in Enamel fluoride uptake (EFU) after brushing for 45 seconds vs. brushing for 2 minutes with 1.5g of experimental dentifrice. [ Time Frame: Baseline and at 14 days ] [ Designated as safety issue: No ]

EFU was measured by using microdrill analysis of the enamel specimens will be carried out after 14 days of intra-oral exposure for each of the toothpaste treatments

Change from baseline in EFU after brushing for 45 seconds vs. brushing for 2 minutes with 0.5g of experimental dentifrice. [ Time Frame: Baseline and at 14 days ] [ Designated as safety issue: No ]

EFU was measured by using microdrill analysis of the enamel specimens will be carried out after 14 days of intra-oral exposure for each of the toothpaste treatments

Change from baseline in EFU after brushing with 0.5g of experimental dentifrice for 2 minutes vs. brushing for 2 minutes with 1.5g of experimental dentifrice. [ Time Frame: Baseline and at 14 days ] [ Designated as safety issue: No ]

EFU was measured by using microdrill analysis of the enamel specimens will be carried out after 14 days of intra-oral exposure for each of the toothpaste treatments

Change from baseline in EFU after brushing with 0.5g of experimental dentifrice for 45 seconds vs. brushing for 45 seconds with 1.5g of experimental dentifrice. [ Time Frame: Baseline and at 14 days ] [ Designated as safety issue: No ]

EFU was measured by using microdrill analysis of the enamel specimens will be carried out after 14 days of intra-oral exposure for each of the toothpaste treatments

Change from baseline in the recovery of % SMH, of brushing for 45 seconds with 0.5g of experimental dentifrice vs. brushing for 45 seconds with 1.5 g of experimental dentifrice. [ Time Frame: Baseline and at 14 days ] [ Designated as safety issue: No ]

Percent SMH recovery (%SMHR) was calculated from hardness values of enamel specimens at baseline (B), after intra-oral exposure (R) and after in vitro demineralization (D1) using formula: [(D1-R)/ (D1-B)]*100. A greater percentage change in SMHR represents a better remineralisation and hence a better outcome.

Change from baseline in the recovery of % SMH, of brushing for 2 minutes with 1.5g of experimental dentifrice vs. brushing for 2 minutes with 1.5 g of an control dentifrice. [ Time Frame: Baseline and at 14 days ] [ Designated as safety issue: No ]

Percent SMH recovery (%SMHR) was calculated from hardness values of enamel specimens at baseline (B), after intra-oral exposure (R) and after in vitro demineralization (D1) using formula: [(D1-R)/ (D1-B)]*100. A greater percentage change in SMHR represents a better remineralisation and hence a better outcome.

Change from baseline in EFU after brushing with 1.5g of experimental dentifrice for 2 minutes vs. brushing for 2 minutes with 1.5g of control dentifrice. [ Time Frame: Baseline and at 14 days ] [ Designated as safety issue: No ]

EFU was measured by using microdrill analysis of the enamel specimens will be carried out after 14 days of intra-oral exposure for each of the toothpaste treatments

Willing to have their denture modified to accommodate enamel test specimens and willing and capable of wearing their removable mandibular partial dentures 24 hours per day during the experimental periods

Antibiotics: Use of antibiotics two weeks prior to a treatment visit or anticipated use during the study treatment periods

Study Scale Use: Subjects unable to measure product weights accurately using the assigned study scale as determined by the study staff (as demonstrated on or before Visit 4)

A member of the site study staff who is directly working on the project or living in that staff's household

Any employee of any toothpaste manufacturer or their spouse or family member

Contacts and Locations

Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study.
To learn more about this study, you or your doctor may contact the study research staff using the Contacts provided below.
For general information, see Learn About Clinical Studies.

Please refer to this study by its ClinicalTrials.gov identifier: NCT01563172