NGS Library Prep Kits

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NEXTflex® library preparation kits are designed to reduce bias and increase the sensitivity, flexibility, and speed of library prep for both Illumina® and Ion Torrent™ sequencing platforms. An extensive selection of NEXTflex single index and dual index barcoded adapters is available to meet your specific multiplexing needs. Automation protocols for the PerkinElmer® Sciclone® NGS Workstation are available for many of the NEXTflex library prep kits for labs requiring higher sample throughput, reduced hands-on time, greater reproducibility, or improved process control.

For research use only. Not for use with diagnostic procedures.

DNA Sequencing Library Prep

An extensive selection of NEXTflex® DNA library preparation kits enables a broad range of applications, including whole-genome sequencing, and library preparation for target enrichment, to meet any of your Illumina® or Ion Torrent™ sequencing needs. The NEXTflex® DNA library prep kits incorporate fast, streamlined workflows that can be used with a broad range of input amounts, and offer multiplexing flexibility to meet the needs of your project.

RNA Sequencing Library Prep

NEXTflex® RNA-Seq Kits are available for both directional and non-directional RNA library prep, with novel reagents and streamlined workflows for Illumina® sequencing platforms. Molecular indexing is incorporated into the NEXTflex® Rapid Directional qRNA-Seq™ Kit and the NEXTflex® qRNA-Seq™ Kit v2 for precise gene expression measurements.

Small RNA Sequencing Library Prep

Severe ligation bias in small RNA sequencing is the primary reason why small RNA sequencing data does not accurately reflect the relative abundance of small RNAs in starting material. The NEXTflex® Small RNA Kits solve this problem by incorporating randomized adapters to reduce ligation bias, offering superior data quality and a greater proportion of productive sequencing reads than any other small RNA library prep protocol. Additionally, the NEXTflex® Small RNA-Seq Kits feature a completely gel-free protocol with normal input amounts and a separate protocol for low-input small RNA library preparation.

Metagenomics

16S and 18S ribosomal RNA (rRNA) sequencing is an amplicon sequencing technique used to identify and compare species from complex microbiome or environmental samples. The NEXTflex® 16S and 18S Amplicon-Seq Kits are optimized and validated library prep kits which offer the ability to multiplex up to 384 libraries, simplifying rRNA sequencing on the Illumina® MiSeq® sequencing platform.

Epigenetics

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Amplicon Panels

An extensive array of NEXTflex® Amplicon Panel Kits is available for sequence variation detection in numerous genes often associated with disorders. These panels are grouped into Inherited Disorders, Newborn and Infertility Syndromes, and Oncology Products. These panels include everything from primer pools, library prep reagents, clean-up beads, and barcodes that is necessary to construct libraries compatible with Illumina® and Ion Torrent™ sequencing platforms. Panels come in convenient reaction sizes that are easily scalable, easy to perform, and cost-effective, which makes them ideal for each lab’s particular needs. Both the workflow and NGS run conditions are equivalent for each panel, thereby allowing simple multiplexing of different panels on a single NGS run to maximize efficiency and minimize cost.

The Sciclone® G3 NGS and NGSx workstations area complete benchtop solution for the automated construction of up to or greater than 96 libraries per day.

Next generation sequencing (NGS) technologies are enabling science at unprecedented rates. As the cost of sequencing declines, throughput continues to increase and the number of applications for which sequencing data can be used is expanding. The Sciclone® G3 NGS and NGSx liquid handling workstation was designed for high throughput, walkaway NGS sample preparation. The deck design and intuitive interface virtually eliminate cross contamination and user error. These liquid handlers simplify the construction of up to or greater than 96 libraries per day.

The JANUS® G3 NGS Express™ workstation is a benchtop platform designed to simplify and reduce errors in low-to-moderate throughput NGS library construction. It uses an intuitive interface to guide users through protocol selection and deck setup, as well as, displays in-run status and details. An optional enclosure not only helps to minimize sample contamination, but the easy to read status lights provide visual feedback for true walkaway sample preparation.