ATP is the primary energy currency of living systems. Virtually all energy requiring processes utilize the chemical energy stored in the phosphate bond of ATP. ATP is formed exclusively in the mitochondria and a variety of genetic diseases can affect ATP formation in the mitochondria. There are a number of commercially available ATP assays which detects femtomoles or less of ATP by measuring luminescence but these kits require specialized luminescence instrumentation and utilize luciferase which can be difficult to maintain in active form.

Catalog #

A0015

ATP Colorimetric and Fluorometric BioAssay™ kit is designed to be a robust, simple method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified by colorimetric (Lmax = 570nm) or fluorometric (Ex/Em = 535/587nm) methods. The assay can detect as low as 50picomol (1uM) of ATP in various samples. The kit provides sufficient reagents for 100 assays.

Kit Components

A0015-01: ATP Assay Buffer: 1x 25ml

A0015-02: ATP Probe (in DMSO): 1x200ul

A0015-04: ATP Converter: 1x1 vial

A0015-05: Developer Mix (lyophilized): 1x1 vial

A0015-06: ATP Standard (1umol; lyophilized): 1x1 vial

Storage and Stability

Store at -20°C. Stable for 6 months at -20°C. A0015-02, A0015-04 and A0015-04 are stable for 2 months once thawed or reconstituted, respectively. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.