Cut pieces
of double stick tape into 25 x 5 mm strips. Adhere two strips
of tape to the center of a slide; they should be side-by-side, 3 mm
apart and parallel to the long axis of the slide.

Retrieve a single squeaky
clean coverslip from the storage jar with Dumont #5 forceps.
Dab the edge of the coverslip to remove any large drops of ethanol,
quickly pass it through the flame of a Bunsen burner, and allow the
ethanol to burn off.

Center the coverslip over
the tape strips on the slide and adhere the coverslip to the strips
of tape, pressing firmly with the back of the forceps to make a good
seal.

Prepare several perfusion
chambers (~20) and store them in a slide storage box until use.