Western Blot: Zic1 Antibody [NB600-488] - Western blot analysis of Zic1 in mouse cerebellum extract. Protein extracts were prepared from mouse cerebellum between postnatal day 5 (P5) and P18, as indicated above the ...read more

Applications/Dilutions

Dilutions

Western Blot 1:1000

Immunocytochemistry/Immunofluorescence 1:10-1:500

Immunohistochemistry 1:400

Immunohistochemistry-Frozen

Immunohistochemistry-Paraffin 1:400

Application Notes

This Zic1 antibody is useful for Western Blot and Immunohistochemisty-paraffin sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Immunohistochemisty-frozen sections was reported in the scientific literature (PMID: 23862012).

Reactivity Notes

Human and mouse. Predicted to react with rat based on 100% sequence homology.

Packaging, Storage & Formulations

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Buffer

PBS and 30% Glycerol

Preservative

0.01% Sodium Azide

Concentration

1.0 mg/ml

Purity

Immunogen affinity purified

Alternate Names for ZIC1 Antibody

Zic family member 1 (odd-paired homolog, Drosophila)

ZIC1

ZICZic family member 1 (odd-paired Drosophila homolog)

Zinc finger protein 201

Zinc finger protein of the cerebellum 1

ZNF201

ZNF201zinc finger protein ZIC 1

Background

Zinc finger protein ZIC 1 (ZIC1) is a member of the ZIC family of C2H2-type zinc finger proteins. These proteins are particularly important during embryonic development. ZIC1 is a transcriptional activator via interaction with GLI1, GLI2, and GLI3. ZIC1 facilitates development and maturation of the cerebellum and spinal cord during organogenesis of the CNS. Within the pontine gray nucleus (PGN) ZIC1 plays a role in spatial distribution of mossy fiber neurons. The interaction between Zic and Gli family proteins are significant for molecular antagonistic and synergistic factors in development. Zic has been shown involvement in modulation of the hedgehog signaling pathway. ZIC1 may be found in the nucleus or in the cytoplasm in the presence of MDFIC overexpression. This protein is mainly found within the CNS and has high levels of expression in the cerebellum. In cancerous tissue, the ZIC1 protein is often found in medulloblastomas. ZIC1 has been used as aneuroepithelial marker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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