IMAC resin

BabyBio NTA

BabyBio™ NTAcolumns for Immobilized Metal Ion Affinity Chromatography (IMAC) are ready-to-use for quick and easy purification of polyhistidine-tagged (His-tagged) proteins and other proteins with an affinity for metal ions. The columns are prepacked with pre-charged or uncharged WorkBeads™ 40 NTA resins. They are available in two column sizes, 1 ml and 5 ml. IMAC utilizes the affinity of histidine, cysteine and tryptophan amino acid side chains on the protein surface for binding to transition metal ions, such as Ni2+, Co2+, Cu2+and Zn2+, which are immobilized via the metal chelating ligand on the chromatography resin.

High binding capacity and purity

Pre-charged resins with different metal ions for convenient screening for optimal purity

Uncharged resins can be charged wih the metal ion of choice

Prepacked ready-to-use columns for rapid and reliable results

Product

The BabyBio™ NTA resins are based on the nitrilotriacetic acid group (NTA) chelating group. The resin can easily be charged, before use,with a broad spectrum of divalent or trivalent transition metal ions, including Ni2+, Co2+, Cu2+or Zn2+, Fe3+or Ga3+.

They can then be used for the Immobilized Metal Ion Affinity Chromatography (IMAC) purification of His-tagged proteins or other proteins with an affinity for metal ions. The selectivity of the metal-charged resin depends on both the choice of ligand (NTA or IDA) and the metal ion used. The uncharged resins can also be used for divalent metal ion removal.

Applications

Bio-Works carries out R&D, manufacturing and supply of agarose-based resins used to separate proteins and other biomolecules. In this large and well-established area, our products stand out as innovative and cutting edge with exceptional performance.