Data extracted from this reference:

Cloned(Commentary)

Commentary

Organism

expression in Escherichia coli

Homo sapiens

Engineering

Amino acid exchange

Commentary

Organism

V88I

functional genetic polymorphism. Mutation results in CBR1 isoforms with different binding affinities for cofactor NADPH and inhibitor rutin as well as different maximal velocities for reaction with daunorubicin and prostaglandin E2

Homo sapiens

KM Value [mM]

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.042

-

menadione

wild-type, 37°C, pH 7.4

Homo sapiens

0.055

-

menadione

mutant V88I, 37°C, pH 7.4

Homo sapiens

0.14

-

daunorubicin

mutant V88I, 37°C, pH 7.4

Homo sapiens

0.173

-

daunorubicin

wild-type, 37°C, pH 7.4

Homo sapiens

0.247

-

NADPH

wild-type, 37°C, pH 7.4

Homo sapiens

0.309

-

prostaglandin E2

wild-type, 37°C, pH 7.4

Homo sapiens

0.32

-

prostaglandin E2

mutant V88I, 37°C, pH 7.4

Homo sapiens

0.343

-

NADPH

mutant V88I, 37°C, pH 7.4

Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]

Molecular Weight Maximum [Da]

Commentary

Organism

32000

-

x * 32000, SDS-PAGE

Homo sapiens

Organism

Organism

Primary Accession No. (UniProt)

Commentary

Textmining

Homo sapiens

-

isoform CBR1

-

Source Tissue

Source Tissue

Commentary

Organism

Textmining

liver

-

Homo sapiens

-

Substrates and Products (Substrate)

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

daunorubicin + NADPH

-

686356

Homo sapiens

daunorubicinol + NADP+

-

-

-

?

menadione + NADPH

-

686356

Homo sapiens

? + NADP+

-

-

-

?

prostaglandin E2 + NADPH

-

686356

Homo sapiens

prostaglandin F2alpha + NADP+

-

-

-

-

Subunits

Subunits

Commentary

Organism

?

x * 32000, SDS-PAGE

Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]

Turnover Number Maximum [1/s]

Substrate

Commentary

Organism

Structure

18

-

prostaglandin E2

mutant V88I, 37°C, pH 7.4

Homo sapiens

25

-

prostaglandin E2

wild-type, 37°C, pH 7.4

Homo sapiens

61

-

daunorubicin

mutant V88I, 37°C, pH 7.4

Homo sapiens

85

-

menadione

mutant V88I, 37°C, pH 7.4

Homo sapiens

91

-

daunorubicin

wild-type, 37°C, pH 7.4

Homo sapiens

110

-

menadione

wild-type, 37°C, pH 7.4

Homo sapiens

149

-

NADPH

mutant V88I, 37°C, pH 7.4

Homo sapiens

179

-

NADPH

wild-type, 37°C, pH 7.4

Homo sapiens

Cofactor

Cofactor

Commentary

Organism

Structure

NADP+

-

Homo sapiens

NADPH

-

Homo sapiens

Cloned(Commentary) (protein specific)

Commentary

Organism

expression in Escherichia coli

Homo sapiens

Cofactor (protein specific)

Cofactor

Commentary

Organism

Structure

NADP+

-

Homo sapiens

NADPH

-

Homo sapiens

Engineering (protein specific)

Amino acid exchange

Commentary

Organism

V88I

functional genetic polymorphism. Mutation results in CBR1 isoforms with different binding affinities for cofactor NADPH and inhibitor rutin as well as different maximal velocities for reaction with daunorubicin and prostaglandin E2