Methods: :
The limbal stem cell graft, cultivated on an amniotic membranesecured to an inter-lockable amnion ring, can be removed fromculture by grasping the plastic amnion ring with forceps andplacing the whole construct onto the diseased eye in one fluidmotion. The limbal biopsy is slightly decentered with respectto the pupil and is left in place. The graft is secured on theeye with fibrin glue and the required size cut out at the levelof the limbus. A second amniotic membrane (AM) basement membranefaced down is placed on top of the graft and acts as a biologicalpatch.

Results: :
The lightweight plastic rings offer stability to the AM andallow it to be transferred onto the surgical field with correctorientation whilst keeping it immobile so that its requireddimensions can be cut out using capsulorhexis scissors.

Conclusions: :
This method for stem cell transplantation is superior to methodscurrently in place since it prevents cell losses incurred dueto manipulation during surgery. The limbal biopsy is not removedfrom the culture and is transplanted attached to the cultivated-limbal-stem-cellgraft; ensuring a continued source of cells and permitting grafttracking in the post operative period.