second step of the polyol pathway of glucose metabolism, important in diabetic disease and hyperglycaemia

655289

Homo sapiens

D-fructose + NADH + H+

-

-

-

?

D-sorbitol + NAD+

i.e. D-glucitol, dissociation of the enzyme-coenzyme binary complex is the rate-limiting step

655289

Mammalia

D-fructose + NADH + H+

-

-

-

?

D-sorbitol + NAD+

i.e. D-glucitol, dissociation of the enzyme-coenzyme binary complex is the rate-limiting step

655289

Rattus norvegicus

D-fructose + NADH + H+

-

-

-

?

D-sorbitol + NAD+

i.e. D-glucitol, dissociation of the enzyme-coenzyme binary complex is the rate-limiting step, 2- and 4-hydroxy groups of D-sorbitol are important for substrate bindig

655289

Ovis aries

D-fructose + NADH + H+

-

-

-

?

D-sorbitol + NAD+

i.e. D-glucitol, dissociation of the enzyme-coenzyme binary complex is the rate-limiting step, interaction of Zn2+ with Ser46 and the oxygen atom of the 2-hydroxy and the 4-hydroxy groups is important for substrate binding

655289

Homo sapiens

D-fructose + NADH + H+

-

-

-

?

additional information

no activity with 2-deoxy-D-sorbitol and 2-fluoro-D-sorbitol, 3-fluoro-D-sorbitol and 4-fluoro-D-sorbitol are poor substrates

1 catalytic zinc atom per subunit, interaction with Ser46 and the oxygen atom of the 2-hydroxy and the 4-hydroxy groups is important for substrate binding

Homo sapiens

Zn2+

1 catalytic zinc atom per subunit

Mammalia

Zn2+

1 catalytic zinc atom per subunit

Ovis aries

Zn2+

1 catalytic zinc atom per subunit, coordination in the active site

Rattus norvegicus

Molecular Weight [Da] (protein specific)

Molecular Weight [Da]

Molecular Weight Maximum [Da]

Commentary

Organism

37000

-

4 * 37000, about

Homo sapiens

37000

-

4 * 37000, about

Mammalia

37000

-

4 * 37000, about

Ovis aries

37000

-

4 * 37000, about

Rattus norvegicus

155000

-

about

Homo sapiens

155000

-

about

Mammalia

155000

-

about

Ovis aries

155000

-

about

Rattus norvegicus

Natural Substrates/ Products (Substrates) (protein specific)

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

D-sorbitol + NAD+

Mammalia

second step of the polyol pathway of glucose metabolism

D-fructose + NADH + H+

-

-

?

D-sorbitol + NAD+

Rattus norvegicus

second step of the polyol pathway of glucose metabolism

D-fructose + NADH + H+

-

-

?

D-sorbitol + NAD+

Ovis aries

second step of the polyol pathway of glucose metabolism

D-fructose + NADH + H+

-

-

?

D-sorbitol + NAD+

Homo sapiens

second step of the polyol pathway of glucose metabolism, important in diabetic disease and hyperglycaemia

D-fructose + NADH + H+

-

-

?

Source Tissue (protein specific)

Source Tissue

Commentary

Organism

Textmining

brain

-

Mammalia

-

erythrocyte

-

Mammalia

-

erythrocyte

-

Homo sapiens

-

lens

-

Mammalia

-

liver

-

Homo sapiens

-

liver

-

Mammalia

-

liver

-

Ovis aries

-

Substrates and Products (Substrate) (protein specific)

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

3-deoxy-D-sorbitol + NAD+

-

655289

Ovis aries

? + NADH

-

-

-

?

6-deoxy-D-sorbitol + NAD+

nearly 2fold higher activity compared to D-sorbitol

655289

Ovis aries

? + NADH

-

-

-

?

6-fluoro-D-sorbitol + NAD+

over 2fold higher activity compared to D-sorbitol, best substrate

655289

Ovis aries

? + NADH

-

-

-

?

D-sorbitol + NAD+

second step of the polyol pathway of glucose metabolism

655289

Mammalia

D-fructose + NADH + H+

-

-

-

?

D-sorbitol + NAD+

second step of the polyol pathway of glucose metabolism

655289

Rattus norvegicus

D-fructose + NADH + H+

-

-

-

?

D-sorbitol + NAD+

second step of the polyol pathway of glucose metabolism

655289

Ovis aries

D-fructose + NADH + H+

-

-

-

?

D-sorbitol + NAD+

second step of the polyol pathway of glucose metabolism, important in diabetic disease and hyperglycaemia

655289

Homo sapiens

D-fructose + NADH + H+

-

-

-

?

D-sorbitol + NAD+

i.e. D-glucitol, dissociation of the enzyme-coenzyme binary complex is the rate-limiting step

655289

Mammalia

D-fructose + NADH + H+

-

-

-

?

D-sorbitol + NAD+

i.e. D-glucitol, dissociation of the enzyme-coenzyme binary complex is the rate-limiting step

655289

Rattus norvegicus

D-fructose + NADH + H+

-

-

-

?

D-sorbitol + NAD+

i.e. D-glucitol, dissociation of the enzyme-coenzyme binary complex is the rate-limiting step, 2- and 4-hydroxy groups of D-sorbitol are important for substrate bindig

655289

Ovis aries

D-fructose + NADH + H+

-

-

-

?

D-sorbitol + NAD+

i.e. D-glucitol, dissociation of the enzyme-coenzyme binary complex is the rate-limiting step, interaction of Zn2+ with Ser46 and the oxygen atom of the 2-hydroxy and the 4-hydroxy groups is important for substrate binding

655289

Homo sapiens

D-fructose + NADH + H+

-

-

-

?

additional information

no activity with 2-deoxy-D-sorbitol and 2-fluoro-D-sorbitol, 3-fluoro-D-sorbitol and 4-fluoro-D-sorbitol are poor substrates