ANCIENT DNA REVEALS INGREDIENTS OF ROMAN MEDECINE

In 130 BC, a ship fashioned from the wood of walnut trees and bulging with medicines and Syrian glassware sank off the coast of Tuscany, Italy. Archaeologists found its precious load 20 years ago and now, for the first time, archaeobotanists have been able to examine and analyse pills that were prepared by the physicians of ancient Greece.

DNA analyses show that each millennia-old tablet is a mixture of more than 10 different plant extracts, from hibiscus to celery.

"For the first time, we have physical evidence of what we have in writing from the ancient Greek physicians Dioscorides and Galen," says Alain Touwaide of the Smithsonian Institution's National Museum of Natural History in Washington DC.

The box of pills was discovered on the wreck in 1989, with much of the medicine still completely dry, according to Robert Fleischer of the Smithsonian's National Zoological Park, also in Washington DC.

Herbal remedies

Fleischer analysed DNA fragments in two of the pills and compared the sequences to the GenBank genetic database maintained by the US National Institutes of Health. He was able to identify carrot, radish, celery, wild onion, oak, cabbage, alfalfa and yarrow. He also found hibiscus extract, probably imported from east Asia or the lands of present-day India or Ethiopia.

"Most of these plants are known to have been used by the ancients to treat sick people," says Fleischer. Yarrow staunched the flow of blood from wounds, and Pedanius Dioscorides, a physician and pharmacologist in Rome in the first century AD, described the carrot as a panacea for a number of problems. "They say that reptiles do not harm people who have taken it in advance; it also aids conception," he wrote around 60 AD.

The concoctions have also thrown archaeobotanists a few curve balls. Preliminary analyses of the ancient pills suggest they contain sunflower, a plant that is not thought to have existed in the Old World before Europeans discovered the Americas in the 1400s. If the finding is confirmed, botanists may need to revise the traditional history of the plant and its diffusion, says Touwaide – but it's impossible for now to be sure that the sunflower in the pills isn't simply from recent contamination.

Quacks no more

Drugs described by Dioscorides and another Greek physician known as Galen of Pergamon have often been dismissed as ineffectual quackery. "Scholars and scientists have often dismissed the literature on such medicines, and expressed doubt about their possible efficacy, which they attributed only to the presence of opium," says Touwaide. He hopes to resolve this debate by exploring whether the plant extracts in the pills are now known to treat illnesses effectively.

He also hopes to discover therian – a medicine described by Galen in the second century AD that contains more than 80 different plant extracts – and document the exact measurements ancient doctors used to manufacture the pills. "Who knows, these ancient medicines could open new paths for pharmacological research," says Touwaide.

The team presented their findings yesterday at the Fourth International Symposium on Biomolecular Archaeology in Copenhagen, Denmark.