In the novel The Sorrows of Empire, Uhura, during a clandestine meeting with Scotty and McCoy, loudly announced that she was going to try to kill Captain Spock. Almost immediately after this proclamation, Uhura disappeared into thin air, a victim of Spock's captured Tantalus field. She was succeeded as communications officer by the mirror universe counterpart of Lieutenant Palmer.

In DC Comics The Mirror Universe Saga, Uhura continued to serve on the Enterprise under Captain Kirk and participates in the Enterprise's incursion into the prime universe. Uhura, along with the rest of the Enterprise crew, were eventually captured by Admiral Kirk and the Enterprise crew and placed into stasis aboard the USS Excelsior. Upon escaping from Excelsior, the Enterprise crew, along with Uhura, were killed by another Terran starship as the Empire had been made to believe that Kirk was a traitor to the Empire, not knowing it was Kirk's counterpart posing as Kirk.