I can’t recommend FloCyte enough to people. I wish everyone who prepares flow samples – even if they don’t run them – would take FloCyte’s courses

Compensation and Multiparameter Strategies

Our first course in Multiparameter Flow Cytometry back in 2004, taught by Dr. Carleton Stewart, who passed on in 2015, was the Director of the Flow Cytometry Lab of Roswell Park Cancer Center in Buffalo, New York. This was a great success. We have continued to build this curriculum begun with Dr. Stewart’s presentations on the basics of antibody binding kinetics, titering antibodies, extra and intracellular staining, using tandem dyes, blocking, and viability and have augmented it with a complete compensation presentation, built on the work of Mario Roederer, Wayne Moore and Dave Parks,and Dr. Bartek Rajwa’s work on spectral unmixing. The response to this course has been excellent!