Why should I use synthetic RNA instead of in vitro transcribed RNA or plasmid expressed guide RNA?

Synthetic RNA can save you up to a week on your CRISPR experiments. Compared to plasmid-based RNA guides, there is no need to design, construct, sequence verify and purify plasmid DNA for every target/guide you want to test. And there’s no need to spend precious lab hours working with expensive IVT kits. Use your extra time to catch up on other experiments!

Accuracy

Have greater control and precision for your CRISPR genome editing by controlling the exact amount of RNA for your transfection or microinjection.

Quality

When forming ribonucleoprotein (RNP) complexes with Cas9, you will get the cleanest possible edits. After transfection and genome editing have occurred, Cas9 and exogenous RNA will degrade. The probability of off-target effects are greatly reduced without continuous expression of Cas9 and/or guide RNA.

Price

Synthetic RNA offers an unbeatable price point when compared to the cost (and labor) of preparing guide RNA through plasmid or IVT methods. Synthego can synthesize cr/tracrRNA and sgRNA at smaller scales than anyone else in the industry – meaning you only pay for what you need.

High Throughput Ready

Synthetic RNA offers the kind of scalability not feasible with other traditional guide RNA generation techniques. Whether you want to test one target per gene or a hundred, it’s as easy as ordering the RNA through our online portal. Synthetic RNA allows you to order libraries of thousands of guide RNAs to test – avoid huge amounts labor and cost involved in creating guides using plasmids or IVT for high throughput applications!

Easy to use

Once a target sequence has been selected it’s easy to order RNA oligos through our online portal – which makes it as simple to order RNA for a single target as it does for a hundred. Once you click order, just sit back, relax and wait for your lyophilized RNA to show up in a few days.