Purpose: :
The previous studies have shown that target- and sequence-independentsiRNA suppressed angiogenesis via stimulation of Toll-Like receptor(TLR) - 3 (Kleinman et al., Nature 452:591-7, 2008). In theirstudies, the mechanism underlying the anti-angiogenesis effectby the siRNA was thought to be involved with IFN-gamma and IL-12.The purpose of this study was to determine the effects of IFN-gammaor IL-12 in the laser-induced CNV model.

Methods: :
We employed recombinant mouse IFN-gamma (10ng, 100ng), recombinantmouse IL-12 (1ng, 10ng). PBS was used for comparison. CNV wasinduced by laser injury in C57BL/6J mice, and volumes measured7 days later by confocal evaluation of Griffonia simplicifoliaIsolectin B4 staining of RPE-choroid flatmounts. IFN-gamma,IL-12 or PBS was injected into the vitreous following injury.Leakage from the CNV was also measured by fluorescein angiography7 days after laser treatment and the lesions were graded basedon the fluorescein leakage as follows: 0 (non leaky), 1 (questionableleakage), 2 (leaky) and 3 (pathologically significant leakage)by masked retina specialists.