Kate,
i did it many (8) years ago in a osteopetrotic transgenic mouse. we did do
long bones to show the increase in the the number of osteoclasts. the
decalcification was slow in EDTA in the cold room. i hand processed the
bones and used a special low melting point wax that we got from Merk in
Germany.
i can't remember the specifics --- the paper citing is:
Soriano P, Montgomery C, Geske R and Bradley A: Targeted disruption of the
c-src proto-oncogene leads to osteopetrosis in mice. Cell 64: 693-702,
1991.
rob
Robert S. Geske
Research Associate
Center for Comparative Medicine and Department of Pediatrics
Baylor College of Medicine
-----Original Message-----
From: Kate_E_Rhodes@sbphrd.com [SMTP:Kate_E_Rhodes@sbphrd.com]
Sent: Wednesday, September 08, 1999 10:23 AM
To: Histonet@pathology.swmed.edu
Subject: TRAP question
Hello histonetters-Has anyone done a Tartrate Resistant Acid Phosphatase
stain on rat decalcified bone that was paraffin embedded?? Did it work???
How did you get it to work??? Any help is greatly appreciated.
Thanks in advance,
Kate Rhodes
SmithKline Beecham
King of Prussia, PA
610-270-7240
FAX 610-270-7202