Most catchments discharging into the Great Barrier Reef (GBR) lagoon have elevated loads of suspended sediment, nutrients, and pesticides, including photosystem II inhibiting herbicides, associated with upstream agricultural land use. To investigate potential impacts of declining water quality on fish physiology, RNASeq was used to characterize and compare the hepatic transcriptomes of barramundi (Lates calcarifer) captured from two of these tropical river catchments in Queensland, Australia. The Daintree and...

Induction of vitellogenin (VTG) in male and immature fish is a standardized endpoint in endocrine disruption testing. In order to establish a nondestructive swab sampling method, VTG induction in the epidermis of Cypriniformes and Perciformes species has been investigated. Both vitellogenin and estrogen receptor genes are expressed in epidermal cells. Immunoaffinity and mass fingerprint analyses show induction of identical VTG peptides in liver and epidermis. Vitellogenin induction by estradiol (E2) and bisphenol...

Phthalic acid esters are frequently detected in aquatic environment. In this study, zebrafish were exposed to low concentrations (0, 0.46, 4.0, and 37.5 µg/L) of mono‐(2‐ethylhexyl) phthalate (MEHP) for 81 days, and the effects on reproduction, gamete quality, plasma vitellogenin (VTG), sex steroids, and transcriptional profiles of key genes involved in steroidogenesis were investigated. The results demonstrated that egg production and sperm quality were decreased upon exposure to MEHP, which also resulted in...

There has been an increasing interest in the use of Acoustic Doppler Current Profilers (ADCPs) to characterise the hydraulic conditions near river engineering structures such as dams, fish passes and groins, as part of ecological and hydromorphological assessments. However, such ADCP applications can be limited by compass errors, obstructed view to navigation satellites, frequent loss of bottom tracking and spatially heterogeneous flow leading to erroneous water velocity measurements. This study addresses these...

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