Sample_hyb_protocol: Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Yeast YG-S98 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.

Sample_scan_protocol: GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.

Sample_description: a nmd2::HIS3 strain harboring the GAL-NMD2 on a plasmid

Sample_data_processing: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.