FGB is the beta component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in its gene lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency.

Catalog #

F4206-01M

Applications

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution

ELISA: 1:10,000

Western Blot: 1:500-1:2000

Immunohistochemistry (Formalin fixed paraffin embedded): 1:200-1:300

Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Clone Type

Monoclonal

Isotype

IgG1

Clone No

1F9

Host

Mouse

Source

Human

Concentration

Not determined

Form

Supplied as a liquid, 0.03% sodium azide.

Purity

Ascites

Immunogen

Recombinant corresponding to aa30-300 of human FGB.

Specificity

Recognizes human FGB.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.