Re: Mast cells.

From:

Barbara Wright

Ian,

Mast cells can be detected in IHC by using Mast Cell Tryptase, or CD117
(c-kit) and the macrophages can be detected by CD68 (all can be purchased
from DAKO). You could also do CD68 for your macrophages and counter stain
after IHC with Toluidine Blue to detect your mast cells.

Hope that helps
Barb Wright

Ian Montgomery wrote:

Any suggestions for a stain or combination
of stains, conventional or IHC, that will enable me to distinguish between
mast cells, macrophages and fibroblasts.
Ian.