MALONATE BROTH

Malonate Broth is a medium used for the differentiation of microorganisms on the basis of their ability to utilize malonate with resultant alkalinity and blue color in the medium.

FORMULAin grams per liter purified filtered water

Yeast Extract

1,0

Ammonium Sulfate

2,0

Potassium Phosphate, dibasic

0,6

Potassium Phosphate, monobasic

0,4

Sodium Chloride

2,0

Sodium Malonate

3,0

Dextrose

0,25

Bromthymol Blue

0,025

pH 6,7 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONSThis medium is for IN VITRO diagnostic use only.

STORAGEStore prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.

SIGN OF DETERIORATIONMedia should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (evaporation or discoloration). Do not use dehydrated media if it is caked.

LIMITATIONS OF METHODAny trace of blue color means positive reaction. A final negative result may be reported only after 48 hours of incubation. This is only a part of the identification. Others tests may be required.