Gentle, tube-based magnetic separation with Invitrogen Dynabeads or MagniSort beads are technologies of choice when you want to isolate high yields of pure, viable and functional cells. Magnetic separation helps ensure that the isolated cells are not affected by passage through a dense column and that the cells’ native characteristics are preserved.

Negative Cell Isolation

In negative isolation, Dynabeads are used to deplete all the unwanted cells using the supplied antibody mix. Your bead-free and untouched target cells are left in the sample, and can be directly analyzed in a flow cytometer and used in any functional assay.

Should you want to refine for a particular cell type, you can of course add extra antibodies to the antibody mix. With the secondary-coated Dynabeads you can create your own negative isolation kit for isolation of any cells from any species.

Depletion

In depletion, cell-specific Dynabeads are used to remove specific unwanted cell types from a mixed cell sample with >99% efficiency. Recover the depleted supernatant - still containing all your wanted cells - for downstream assays. Click for more info on: Dynabeads for human cell depletion and mouse cell depletion.

You can also use the positively targeted and bead-bound cells for downstream molecular applications that do not require release of cells.

The Two Worlds of Cell Separation

An entertaining and useful explanation of the differences between the micro- and nano-particle techniques used for cell isolation / cell separation.