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Abstract

BackgroundSeveral studies have described an enhanced inflammatory status and oxidative stress
balance disruption in women with polycystic ovary syndrome (PCOS). However, there
is scarce information about redox markers in the blood of androgenized animal models.
Here, we evaluated the serum/plasma oxidative stress marker and metabolic parameter
characteristics of prenatal (PreN) and postnatal (PostN) androgenized rat models of
PCOS.Materials and methodsFor PreN androgenization (n=8), 2.5 mg of testosterone propionate was subcutaneously
administered to dams at embryonic days 16, 17, and 18, whereas PostN androgenization
(n=7) was accomplished by subcutaneously injecting 1.25 mg of testosterone propionate
to animals at PostN day 5. A unique control group (n=8) was constituted for comparison.ResultsOur results indicate that PostN group rats exhibited particular modifications in the
oxidative stress marker, an increased plasma ferric-reducing ability of plasma, and
an increased antioxidant capacity reflected by higher albumin serum levels. PostN
animals also presented increased total cholesterol and triglyceride–glucose levels,
suggesting severe metabolic disarrangement.ConclusionStudy findings indicate that changes in oxidative stress could be promoted by testosterone
propionate exposure after birth, which is likely associated with anovulation and/or
lipid disarrangement.

A simple, automated test measuring the ferric reducing ability of plasma, the FRAP assay, is presented as a novel method for assessing "antioxidant power." Ferric to ferrous ion reduction at low pH causes a colored ferrous-tripyridyltriazine complex to form. FRAP values are obtained by comparing the absorbance change at 593 nm in test reaction mixtures with those containing ferrous ions in known concentration. Absorbance changes are linear over a wide concentration range with antioxidant mixtures, including plasma, and with solutions containing one antioxidant in purified form. There is no apparent interaction between antioxidants. Measured stoichiometric factors of Trolox, alpha-tocopherol, ascorbic acid, and uric acid are all 2.0; that of bilirubin is 4.0. Activity of albumin is very low. Within- and between-run CVs are <1.0 and <3.0%, respectively, at 100-1000 micromol/liter. FRAP values of fresh plasma of healthy Chinese adults: 612-1634 micromol/liter (mean, 1017; SD, 206; n = 141). The FRAP assay is inexpensive, reagents are simple to prepare, results are highly reproducible, and the procedure is straightforward and speedy. The FRAP assay offers a putative index of antioxidant, or reducing, potential of biological fluids within the technological reach of every laboratory and researcher interested in oxidative stress and its effects.

To develop a new, colorimetric and automated method for measuring total oxidation status (TOS). The assay is based on the oxidation of ferrous ion to ferric ion in the presence of various oxidant species in acidic medium and the measurement of the ferric ion by xylenol orange. The oxidation reaction of the assay was enhanced and precipitation of proteins was prevented. In addition, autoxidation of ferrous ion present in the reagent was prevented during storage. The method was applied to an automated analyzer, which was calibrated with hydrogen peroxide and the analytical performance characteristics of the assay were determined. There were important correlations with hydrogen peroxide, tert-butyl hydroperoxide and cumene hydroperoxide solutions (r=0.99, P<0.001 for all). In addition, the new assay presented a typical sigmoidal reaction pattern in copper-induced lipoprotein autoxidation. The novel assay is linear up to 200 micromol H2O2 Equiv./L and its precision value is lower than 3%. The lower detection limit is 1.13 micromol H2O2 Equiv./L. The reagents are stable for at least 6 months on the automated analyzer. Serum TOS level was significantly higher in patients with osteoarthritis (21.23+/-3.11 micromol H2O2 Equiv./L) than in healthy subjects (14.19+/-3.16 micromol H2O2 Equiv./L, P<0.001) and the results showed a significant negative correlation with total antioxidant capacity (TAC) (r=-0.66 P<0.01). This easy, stable, reliable, sensitive, inexpensive and fully automated method that is described can be used to measure total oxidant status.

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