Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Applications

Approved:

Western blot (5 µg/ml)

Flow Cytometry (5 - 20 µg/ml)

Neutralization

Specificity and Use

Immunogen:

CD95 / FAS antibody was raised against recombinant human Fas.

Specificity:

Recognizes the human cell surface antigen Fas expressed in various human cells, including myeloid cells, T lymphoblastoid cells and diploid fibroblasts. Does not induce apoptosis in cell culture. Species cross-reactivity: Does not react with TNF and mouse.

Usage:

Suitable for use in Neutralization, Flow Cytometry and Western Blot. Western Blot: 5 ug/ml. Flow Cytometry: 5-20 ug/ml. Neutralization: 10-500ng/ml of this antibody was incubated for one hour with human Fas- transfected cells and inhibited apoptosis induced by the anti-Fas (human, activating) clone CH11/clone 0.T.45 treatment of the cells. Cell viability was assessed using a WST-1 assay.

Description: CD95 / FAS is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10.