DETECTION OF A SINGLE GENETICALLY MODIFIED BACTERIAL-CELL IN SOIL BY USING CHARGE COUPLED DEVICE-ENHANCED MICROSCOPY

Abstract

Genes encoding bioluminescence from Vibrio harveyi were cloned into Pseudomonas syringae pv. phaseolicola, resulting in high levels of bioluminescence. After inoculation of sterile and nonsterile soil slurries with bioluminescent P. syringae, cells could not be identified by conventional light microscopy. However, when we used charge coupled device-enhanced microscopy, bioluminescent single cells were detected easily in dark fields despite masking by soil particulate matter, and in addition, the extent of competition from indigenous soil bacteria could be monitored. The technique which we describe offers great potential for tracking and determining the spatial distribution of genetically marked microorganisms in the environment.

title = "DETECTION OF A SINGLE GENETICALLY MODIFIED BACTERIAL-CELL IN SOIL BY USING CHARGE COUPLED DEVICE-ENHANCED MICROSCOPY",

abstract = "Genes encoding bioluminescence from Vibrio harveyi were cloned into Pseudomonas syringae pv. phaseolicola, resulting in high levels of bioluminescence. After inoculation of sterile and nonsterile soil slurries with bioluminescent P. syringae, cells could not be identified by conventional light microscopy. However, when we used charge coupled device-enhanced microscopy, bioluminescent single cells were detected easily in dark fields despite masking by soil particulate matter, and in addition, the extent of competition from indigenous soil bacteria could be monitored. The technique which we describe offers great potential for tracking and determining the spatial distribution of genetically marked microorganisms in the environment.",

T1 - DETECTION OF A SINGLE GENETICALLY MODIFIED BACTERIAL-CELL IN SOIL BY USING CHARGE COUPLED DEVICE-ENHANCED MICROSCOPY

AU - SILCOCK, D J

AU - WATERHOUSE, R N

AU - Glover, Lesley Anne

AU - Prosser, James Ivor

AU - Killham, Kenneth Stuart

PY - 1992/8

Y1 - 1992/8

N2 - Genes encoding bioluminescence from Vibrio harveyi were cloned into Pseudomonas syringae pv. phaseolicola, resulting in high levels of bioluminescence. After inoculation of sterile and nonsterile soil slurries with bioluminescent P. syringae, cells could not be identified by conventional light microscopy. However, when we used charge coupled device-enhanced microscopy, bioluminescent single cells were detected easily in dark fields despite masking by soil particulate matter, and in addition, the extent of competition from indigenous soil bacteria could be monitored. The technique which we describe offers great potential for tracking and determining the spatial distribution of genetically marked microorganisms in the environment.

AB - Genes encoding bioluminescence from Vibrio harveyi were cloned into Pseudomonas syringae pv. phaseolicola, resulting in high levels of bioluminescence. After inoculation of sterile and nonsterile soil slurries with bioluminescent P. syringae, cells could not be identified by conventional light microscopy. However, when we used charge coupled device-enhanced microscopy, bioluminescent single cells were detected easily in dark fields despite masking by soil particulate matter, and in addition, the extent of competition from indigenous soil bacteria could be monitored. The technique which we describe offers great potential for tracking and determining the spatial distribution of genetically marked microorganisms in the environment.