The process of cell acquisition and culture is an important part of tissue engineering construction. Three-dimensional bioprinting technology can support the development of tissue engineering. Piezoelectric micro-jetting realize pulsating flow of trace fluid under the interaction of the inertial force and fluid viscous force by “sound wave”, its application in the field of cell bioprinting belongs to the emerging technology, and it has the characteristics of high precision, high efficiency as well as low cost. Based on the introduction of micro-jetting technology system and principle, the effects of piezoelectric drive mode, piezoelectric parameters, pulse drive voltage waveform and bio-cell ink on cell bioprinting were analyzed, the cases studied of piezoelectric micro-jetting technology in high livability cell acquisition and efficient construction of cell three-dimensional tissue were provided, its application status in the field of cell bioprinting and research direction as well as significance were summarized.