National average yield of wheat in Pakistan is only 2.54 tons per hectare. One of the main reason
for low yield of wheat in Pakistan is leaf rust disease. During present study, RAPD based molecular markers
were developed for three leaf rust resistance genes (Lr47, Lr37 and Lr51) incorporated to wheat from wild
relatives. Three sets of near isogenic lines (Anza, Anza+Lr37, Kern, kern+Lr47, Yecora Rojo and Yecora
Rojo+Lr51) of wheat were used. A total of 12 RAPD primers were used to identify alien rust resistant genes.
One (OPA07), three (OPA12, OPA15, OPB11) and two (OPA11, OPB11) RAPD primers were identified which
produced specific bands for alien rust resistant genes Lr47, Lr51 and Lr37, respectively.