pE-100

Single-wavelength systems for fluorescence screening applications

Overview

The pE-100 series is a family of single-wavelength fluorescence LED illumination systems, which are compact and simple to use. Systems can be specified at any one of 20 different LED wavelengths, and are ideal for routine clinical screening, electrophysiology or basic research applications. Operation is by a remote manual control pod with instant on/off and intensity control from 0-100%, with remote control available via a TTL trigger.

The pE-100 system is designed for fluorescence applications requiring a single LED excitation wavelength. It is perfect for clinical applications such as routine screening (e.g. using Auramine for Tuberculosis), for research applications requiring precise intensity control and fast-switching, or for electrophysiology applications where light has to be delivered to a specific location. The user can select from 20 different LED wavelengths, ranging from the near-UV at 365 nm to the near-IR at 770 nm. For increased intensity, a direct fit version is also available, which fits directly to the microscope via a specified adaptor.

Plus all the benefits of LED technology.

The system comprises a pE-100 LED Light Source, control pod, and power supply.

Control

Control pod for operation and access to settings and usage history.

Manual:Manual control for instant on/off and intensity control in 1% steps from 0 – 100%

Remote:Via single TTL for on/off control using a BNC connection on the control pod. Triggering speed approx. 150 µs

Choosing configurations

There are three standard pE-100 configuration options:

Direct-fit: The pE-100 is designed to connect directly to a microscope’s epi-illumination port by selecting from a range of microscope adaptors. A simple once only adjustment will allow optimisation to the optical path of the microscope (watch the tutorial video here).

Fibre: The pE-100fiber features an SMA connection for accepting multimode fibres and has been designed with efficient coupling into a wide range of fibre types which can be sourced independently or purchased through CoolLED (see accessories). The pE-100fiberalso includes excitation filter holders.

Combining pE-100 Light Sources

Direct fit configuration

pE-100 units can be combined using the pE-Combiner for applications requiring a second LED wavelength with independent control and triggering.

I originally approached various companies with regards to getting an LED light source for my Leica DMLB microscope. The fact that the microscope was old and had a mechanised stage meant that some of the LED systems that we looked at were unsuitable to be fitted. Thankfully the CoolLED system simply slots in where the old mercury bulb unit used to sit. It took less than five minutes to fit, and was ready to use once it was switched on. No more waiting for the bulb to get to temperature, no more bulb changing, or re-calibrating of the mirrors before use. Simply marvellous!

Kam Yim Wong, Immunology Dept, Derriford Hospital, Plymouth UK

We have had great results using the CoolLED pE-100 on our TB, fungus and bacteria screening microscopes and find the CoolLED LED products make screening quick and easy.

Richard Wills, Microbiology Laboratory Manager at The Royal Berkshire Hospital

The main benefit of CoolLED's light source is the ability to switch on and off when required and also not having to align or change a bulb every 300 hours. Over the years it will pay for itself when compared to the cost of mercury bulbs.

pE-Universal Collimator

Fibres

Liquid Light Guide

pE-Combiners

pE-Dual Adapt

Adaptors

Environment & Safety

Did you know that LED illumination systems help laboratories reduce their carbon footprint, protecting laboratory staff along the way?

Toxic mercury-based microscope illuminators are bad news for the environment, draw a lot of power and have a short lifetime.

LED illumination systems are cleaner, have a long lifetime and use less power. Our pE-300 Series is also ACT label certified and is a natural choice for labs who want to play their part in helping the environment.

Help & Advice

At CoolLED we are committed to giving our customers outstanding service right from the start and throughout the lifetime of the product. Please get in touch if you have any questions or would like a quote. You can also browse our site for technical resources and general support.