Colony stimulating activity present in plasma and in leukocytes from chronic myelogenous leukemia patients and from normal volunteers was determined by double layer bone-marrow tissue culture. Plasma and leukocytes incorporated into the bottom feeder layer stimulated the formation of colonies from human bone-marrow cells cultured in the upper semi-solid agar layer. The colony stimulating activity of plasma from chronic myelogenous leukemia patients was significantly increased over that of normal plasma (p<.01). The difference between the colony stimulating activity of chonic myelogenous leukemia and normal leukocytes was not statistically significant. After 14 days of incubation, all bone-marrow cell colonies were composed primarily of macrophages rather than granulocytes as had been previously reported.

Description:

Author Institution: Leukemia Research Laboratory, Department of Biological Sciences, Bowling Green State University

URI:

http://hdl.handle.net/1811/22409

ISSN:

0030-0950

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