The fitting of exponentials to relaxation curves (relaxation curve-fitting or as used throughout this chapter abbreviated simply as relax-fit) involves a number of steps including the loading of data, the calculation of both the average peak intensity across replicated spectra and the standard deviations of those peak intensities, selection of the experiment type, optimisation of the parameters of the exponential curves during the fit for each observed spin, Monte Carlo simulations to find the parameter errors, and saving and viewing the results.
To simplify the process a sample script will be followed step by step as was done with the NOE calculation.