Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

WB

1/500 - 1/2500. Predicted molecular weight: 79 kDa.

IP

Use at 2-10 µg/mg of lysate.

Target

Tissue specificityLiver and kidney. Lower amounts seen in the brain.

PathwayLipid metabolism; fatty acid beta-oxidation.

Sequence similaritiesIn the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Post-translationalmodificationsAcetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids.