Abstract: Iron chelation therapy can be used for the selective removal of Fe3+ ions from spiked human plasma by ion imprinting. N-Methacryloyl-(L)-glutamic acid (MAGA) was chosen as the chelating monomer. In the first step, MAGA was complexed with the Fe3+ ions to prepare the precomplex, and then the ion-imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-glutamic acid) [PHEMAGA-Fe3+] cryogel column was prepared by cryo-polymerization under a semi-frozen temperature of -12°C for 24 h. Subsequently, the template, of Fe3+ ions was removed from the matrix by using 0.1 M EDTA solution. The values for the specific surface area of the imprinted PHEMAGA-Fe3+ and non-imprinted PHEMAGA cryogel were 45.74 and 7.52 m2/g respectively, with a pore size in the range of 50 - 200 μm in diameter. The maximum Fe3+ adsorption capacity was 19.8 μmol Fe3+/g cryogel from aqueous solutions and 12.28 μmol Fe3+/g cryogel from spiked human plasma. The relative selectivity coefficients of ion-imprinted cryogel for Fe3+/Ni2+ and Fe3+/Cd2+ were 1.6 and 4.2-fold greater than the non-imprinted matrix, respectively. It means that the PHEMAGA-Fe3+ cryogel possesses high selectivity to Fe3+ ions, and could be used many times without significantly decreasing the adsorption capacityAuthor keywords:Affinity, Cryogel, iron removal, metal detoxification, Molecular recognition