Ratio between cells and lysis buffer - (Sep/03/2008 )

Hi everyoneI am going to prepare a cell lysate from a cell line, but I have no idea on how much lysis buffer I should use to lysis the cells. Is there any ratio between no. of cells and the lysis buffer? Here is a recipe for my lysis buffer:

Hi everyoneI am going to prepare a cell lysate from a cell line, but I have no idea on how much lysis buffer I should use to lysis the cells. Is there any ratio between no. of cells and the lysis buffer? Here is a recipe for my lysis buffer: