Bottom Line:
RESULTS The patients and controls did not differ significantly in terms of any characteristic except age.The results showed no significant difference regarding biallelic 5-HTTLPR.Based on another classification by expression, we found a significantly lower frequency of the L'L' genotype (OR=0.37; 95%CI=0.15-0.91, P=0.025) in patients with LPE.

ABSTRACTBACKGROUND This study aimed to explore the relationship between premature ejaculation (PE) and the serotonin transporter gene-linked polymorphic region (5-HTTLPR) with respect to the biallelic and triallelic classifications. MATERIAL AND METHODS A total of 115 outpatients who complained of ejaculating prematurely and who were diagnosed as having lifelong premature ejaculation (LPE) and 101 controls without PE complaint were recruited. All subjects completed a detailed questionnaire and were genotyped for 5-HTTLPR polymorphism using PCR-based technology. We evaluated the associations between 5-HTTLPR allelic and genotypic frequencies and their association with LPE, as well as the intravaginal ejaculation latency time (IELT) of different 5-HTTLPR genotypes among LPE patients. RESULTS The patients and controls did not differ significantly in terms of any characteristic except age. The results showed no significant difference regarding biallelic 5-HTTLPR. According to the triallelic classification, no significant difference was found when comparing the genotypic distribution (P=0.091). However, the distribution of the S, LG, and LA alleles in the cases was significantly different from the controls (P=0.018). We found a significantly lower frequency of LA allele and higher frequency of LG allele in patients. Based on another classification by expression, we found a significantly lower frequency of the L'L' genotype (OR=0.37; 95%CI=0.15-0.91, P=0.025) in patients with LPE. No significant association was detected between IELT of LPE and different genotypes. CONCLUSIONS Contrary to the general classification based on S/L alleles, triallelic 5-HTTLPR was associated with LPE. Triallelic 5-HTTLPR may be a promising field for genetic research in PE to avoid false-negative results in future studies.

Mentions:
The current study was carried out between October 2012 and March 2015. A flow chart of participant enrollment and data collection is shown in Figure 1. Each participant was informed of the purpose of the study and signed the informed consent. In the initial evaluation, each subject completed a detailed face-to-face interview with an andrologist, including a questionnaire and physical examination. The questionnaire included the following items: (I) demographic and clinical characteristics, (II) duration of relationship and marital status; and (III) self-estimated IELTs. The IELT was the time from the insertion of the penis into the vagina to the start of intravaginal ejaculation. Every participant was asked to give an estimation of the IELTs, which were called self-estimated IELTs. LPE in our study was defined according to the definition of ISSM. Finally, 115 patients with LPE were recruited by referral from the Andrology Outpatient Clinic. At the same time, 101 controls without PE complaint were enrolled from the medical examination center. Patients diagnosed as having LPE were required to measure the IELTs by stopwatch at least 4 times during 1 month.

Mentions:
The current study was carried out between October 2012 and March 2015. A flow chart of participant enrollment and data collection is shown in Figure 1. Each participant was informed of the purpose of the study and signed the informed consent. In the initial evaluation, each subject completed a detailed face-to-face interview with an andrologist, including a questionnaire and physical examination. The questionnaire included the following items: (I) demographic and clinical characteristics, (II) duration of relationship and marital status; and (III) self-estimated IELTs. The IELT was the time from the insertion of the penis into the vagina to the start of intravaginal ejaculation. Every participant was asked to give an estimation of the IELTs, which were called self-estimated IELTs. LPE in our study was defined according to the definition of ISSM. Finally, 115 patients with LPE were recruited by referral from the Andrology Outpatient Clinic. At the same time, 101 controls without PE complaint were enrolled from the medical examination center. Patients diagnosed as having LPE were required to measure the IELTs by stopwatch at least 4 times during 1 month.

Bottom Line:
RESULTS The patients and controls did not differ significantly in terms of any characteristic except age.The results showed no significant difference regarding biallelic 5-HTTLPR.Based on another classification by expression, we found a significantly lower frequency of the L'L' genotype (OR=0.37; 95%CI=0.15-0.91, P=0.025) in patients with LPE.

ABSTRACTBACKGROUND This study aimed to explore the relationship between premature ejaculation (PE) and the serotonin transporter gene-linked polymorphic region (5-HTTLPR) with respect to the biallelic and triallelic classifications. MATERIAL AND METHODS A total of 115 outpatients who complained of ejaculating prematurely and who were diagnosed as having lifelong premature ejaculation (LPE) and 101 controls without PE complaint were recruited. All subjects completed a detailed questionnaire and were genotyped for 5-HTTLPR polymorphism using PCR-based technology. We evaluated the associations between 5-HTTLPR allelic and genotypic frequencies and their association with LPE, as well as the intravaginal ejaculation latency time (IELT) of different 5-HTTLPR genotypes among LPE patients. RESULTS The patients and controls did not differ significantly in terms of any characteristic except age. The results showed no significant difference regarding biallelic 5-HTTLPR. According to the triallelic classification, no significant difference was found when comparing the genotypic distribution (P=0.091). However, the distribution of the S, LG, and LA alleles in the cases was significantly different from the controls (P=0.018). We found a significantly lower frequency of LA allele and higher frequency of LG allele in patients. Based on another classification by expression, we found a significantly lower frequency of the L'L' genotype (OR=0.37; 95%CI=0.15-0.91, P=0.025) in patients with LPE. No significant association was detected between IELT of LPE and different genotypes. CONCLUSIONS Contrary to the general classification based on S/L alleles, triallelic 5-HTTLPR was associated with LPE. Triallelic 5-HTTLPR may be a promising field for genetic research in PE to avoid false-negative results in future studies.