Tetracycline-Inducible Expression-Tet-Off Cell Lines

Tet-Off and Tet-Off Advanced inducible cell lines are host cell lines that greatly simplify the task of setting up tetracycline-inducible gene expression systems which are activated by removing doxycycline (Dox) from the culture medium (1, 2). Our Tet-On Advanced and Tet-On inducible cell lines are also available, which are activated by adding Dox to the culture medium.

These rigorously tested clonal cell lines:

Stably express either the second-generation Tet-Off Advanced transactivator protein or the original Tet-Off transactivator protein

Allow you to induce high-level expression of your gene-of-interest by removing (Dox) from the culture of transfected cells

Can save you up to two months of time in developing a complete Tet-controlled system.

Easily create an inducible system by first placing your gene under control of a tetracycline-responsive promoter (e.g. PTRE3G), transfecting it into one of these G418-resistant cell lines, and selecting for double-stable clones.

Features

Applications

Inducible gene expression in mammalian cells

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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