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BDNF Emax® ImmunoAssay Systems

The BDNF Emax® ImmunoAssay Systems provide optimized reagents and a protocol for the sensitive and specific detection of brain-derived neurotrophic factor (BDNF). After an overnight coating of a 96-well plate, the specific protein is detected using an antibody sandwich format. The systems use a horseradish peroxidase-conjugated secondary antibody and a single-component TMB substrate for the final chromogenic detection of bound neurotrophic factor. Using this system, BDNF in tissue culture supernatants, tissue homogenates, plasma and urine can be quantitated in the range of 7.8–500pg/ml. Binding and recovery from m...

The BDNF Emax® ImmunoAssay Systems provide optimized reagents and a protocol for the sensitive and specific detection of brain-derived neurotrophic factor (BDNF). After an overnight coating of a 96-well plate, the specific protein is detected using an antibody sandwich format. The systems use a horseradish peroxidase-conjugated secondary antibody and a single-component TMB substrate for the final chromogenic detection of bound neurotrophic factor. Using this system, BDNF in tissue culture supernatants, tissue homogenates, plasma and urine can be quantitated in the range of 7.8–500pg/ml. Binding and recovery from mouse brain homogenates has not been fully characterized.

Features - Benefits

High Value: Optimized reagents and protocol provided.

Specificity: Specific detection of BDNF; less than 3% cross-reactivity with other related neurotrophic and growth factors.

Sensitivity: Detect picogram levels of factor per milliliter of sample.

Flexibility: Available kits provide sufficient reagents for two or five 96-well plates; you can configure your plates as desired.

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.

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We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.