About mercaptoethanol

One can understand why it might be mistaken for a denaturant though, as it
breaks disulfide bridges in proteins, many of which are essential to
maintain tertiary structure, and possibly secondary structure too, so a
protein with its disulfides broken will be more likely to unfold, thus
making it more accessible to another denaturant that's probably in your
buffer, like SDS.
PSB
_________________________________________
Dr. Paul S. Brookes. (brookes at uab.edu)
UAB Department of Pathology, G004 Volker Hall
1670 University Blvd., Birmingham AL 35294 USA
Tel (001) 205 934 1915 Fax (001) 205 934 1775
http://peir.path.uab.edu/brookes
The quality of e-mails can go down as well as up
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