What are CherryPicker systems?

CherryPicker systems are powerful sample preparation tools for monitoring and capturing a homogeneous population of cells that either express a specific protein or express a specific promoter activity. These systems utilize the chimeric membrane-anchored CherryPicker fluorescent protein, which can be monitored via fluorescence microscopy and captured on magnetic beads via a specific antibody.

Separate cells that have been transfected at a low level from untransfected cells

Create a homogeneous cell population based on promoter activity or protein expression

Capture and enrich your cells in <2 hr using a bench-top protocol, instead of expensive, time-consuming sorting using flow cytometry

Protocol overview

How do CherryPicker systems work?

The CherryPicker reporter is only expressed together with your protein of interest (Video 1), or downstream of your promoter of interest (Video 2). Therefore, only the cells with your activity of interest will be enriched. Watch our videos below to see how you can use CherryPicker for both applications.

CherryPicker kit components and buying guide

Each CherryPicker system includes, 2 vectors (including control vector), CherryPicker antibody, buffers, and magnetic beads (magnetic stand is available separately). Use the table below to choose the right kit for your application.

Product

Comments

Systems for Promoter Studies (vectors included)

CherryPicker Cell Capture System

Cat. No. 632572

Contains the CherryPicker Reagent Kit, magnetic beads, a promoterless CherryPicker vector, and a control vector

Promoterless system suitable for use with most cell types. Use the CherryPicker Cell Capture System to capture and enrich cells in which your promoter of interest is active.

Lenti-X CherryPicker Cell Capture System

Promoterless system which can be used to transduce virtually any dividing or nondividing cells, including primary cells and stem cells. Use the lentiviral CherryPicker Cell Capture System to capture and enrich cells in which your promoter of interest is active.

Systems for Protein Studies (vectors included)

CherryPicker Cell Capture System (IRES)

Cat. No. 632573

Contains the CherryPicker Reagent Kit, magnetic beads, a bicistronic CherryPicker vector, and a control vector

Bicistronic system suitable for use with most cell types. Use the CherryPicker Cell Capture System (IRES) to capture and enrich cells expressing your protein of interest.

Lenti-X CherryPicker Cell Capture System (IRES)

Bicistronic system which can be used to transduce virtually any dividing or nondividing cells, including primary cells and stem cells. Use the CherryPicker Cell Capture System (IRES) to capture and enrich cells expressing your protein of interest.

* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.