Microscopy product ID: 3793

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2D Image

Description

Projection through optical section series of the astrocyte filled with AlexaFluor 568 in the adult rat dentate gyrus. Saturated pixels are displayed in red. This section was also double labeled with AlexaFluor 488 to reveal N-CAM (see merged image under reconstruction).

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46.4 MB

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Description

Zip file containing original optical section series through a protoplasmic astrocyte injected with AlexaFluor 568(slc5as4_ly.pic) in dentate gyrus where N-CAM was labeled with AlexaFluor 488 (slc5as4_ncam.pic). Each label is in a separate file. The merged file is available for download under "Reconstruction."

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Animation through optical section series of the astrocyte filled with AlexaFluor 568 in the molecular layer of the adult rat dentate gyrus imaged using confocal microscopy. Tissue was immunolabeled with AlexaFluor 488 to reveal N-CAM (merged data set is under "Reconstruction")

Reconstruction

Description

Projection through merged optical section series of the astrocyte filled with AlexaFluor 568 (green) in the molecular layer of the dentate gyrus, stained with AlexaFluor 488 to reveal N-CAM (red), showing the relationship between astrocyte processes and laminar boundaries revealed by N-CAM staining. Contrast was enhanced for better viewing.

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Animation through the optical sections of a confocal data set showing the relationship of the astrocyte filled with AlexaFluor 568 (green) to laminar boundaries in the dentate gyrus revealed by staining N-CAM with AlexaFluor 488(red) immunolabeling. the animation has been downsampled from the original data for ease of display.

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