Synthesis and Conformational Analysis of Some Erythropoietin Mimetic Peptides Using Microwave Energy

THE CURRENT study describes the synthesis of Erythropoietin- ......which is a prohibited hormone by WADA-mimetic peptides [(AFIII54(chain I), (Ser)12AFIII54(chain II), AFIII54 with addition of Gly and Tyr in position 4 and 5, respectively (chain VI), AFIII54 devoid (Thr)9 and )Trp)10 (chain VII), (EMP3(chain III),(Ser)15EMP3 (chain IV), (Ala)15EMP3(chain V), EMP3 with addition of Gly and Tyr in position 7 and 8, respectively (chain VIII) and EMP3 devoid (Thr)12and (Trp)13(chain IX) ] by SPPS using microwave energy which accelerated reaction rate and improved the purities and yield.
Conformational analysis using FT-IR spectroscopy showed that most the synthesised peptides existed in α-helix structure. Also, biological activity for one of these sequences was studied.