This was posted today:
>I need to quantitate pmoles of T7 synthesized 32P-labelled RNA, as well as
>unlabelled invitro synthesized RNA. What are the preferred methods, do you
>gel purify the RNA, or NH4Ac ppt from the synthesis rxn? thanks
You need to get rid of unincorporated counts in order to accurately
quantitate your RNAs. In my hands, gel purification is the best method and
the only one I trust. I use tritium labelled RNA as "unlabelled" and run it
next hot stuff so that I can cut it out of a gel.
I will be happy to share with you my entire protocol if you will send me
your e-mail address.
Brian Zeiler
brianz at microbio.lifesci.ucla.edu