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All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

If I want to perform a Western blot using the &alpha;-Actin Antibody (1A4) on human samples, what secondary reagent should I use for detection?

Asked by: Trav11

We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgG&kappa; BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here:
https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety

Answered by: Tech Service 8

Date published: 2016-12-16

Can I use this mouse monoclonal antibody for triple staining even if my other primary antibodies were also raised in mice?

I would like to know whether this antibody is usable to evaluate the expression pattern of alfa-actin protein in adult zebrafish especially skeletal muscles. If not, could you please tell us the other useful antibodies?

Asked by: Yosuke

Thank you for your question. &alpha;-Actin Antibody (1A4): sc-32251 is recommended for detection of smooth muscle &alpha;-Actin, rather than skeletal muscle actin isoforms. This antibody has not yet been tested in zebrafish, and it is unknown if it would work for that species.
As a recommended alternative, we would suggest our mouse monoclonal, &beta;-Actin Antibody (C4): sc-47778. This antibody is recommended for detection of &beta;-Actin; may cross-react with all six known isoforms of Actin (including cytoplasmic &beta;- and &gamma;- Actin isoforms, skeletal, cardiac, and vascular &alpha;-Actin isoforms, and enteric &gamma;-Actin isoform). This antibody has also been cited in the literature used in zebrafish in the following articles on NCBI's PubMed: PMID: 25075304, 22139372.

Rated 5 out of
5 by
tdominko from
Good for WB, IF, FACSThis antibody was used successfully for immunofluorescence (1:100), Western blot (1:500), and indirect flow cytometry (1:200). Good signal and specificity with very little background.

Date published: 2017-09-14

Rated 5 out of
5 by
Sally Du from
Useful antibody!I bought this antibody a year ago and use it in WB analyse. This a-Actin antibody could specificly recognise a-SMA(a-smooth muscle actin).a-SMA is quite important to my study on liver fibrosis and hepatic stellate cells.In my experiment,I use the antibody to analyse a-SMA protein expression in cell extracts and mouse liver tissues.I incubated the PVDF membrane with a-actin antibody in a dilution of 1:1000 for a whole night. It is amazing!

Date published: 2017-09-01

Rated 5 out of
5 by
ruanjj from
good antibodyThis is a trial product, which I was applied for and It's very great !

Date published: 2017-08-26

Rated 5 out of
5 by
seal from
good antibodyThis antibody shows a robust clear band and it is perfect

Date published: 2017-07-25

Rated 5 out of
5 by
Torres from
WB analysisFor mouse brain tissue analysis, there was band detected by a-actin antibody the molecular weight around 43KDa.No background .

Date published: 2017-07-08

Rated 5 out of
5 by
小新贝贝 from
Very nice!1:1000 diluted for wb of protein extracted from h9c2 cell,the band is so strong!

Date published: 2017-07-02

Rated 5 out of
5 by
Yana from
Wonderful a-Actin Sc-32251I am keeping using a Actin Sc-32251 for several years. I like it so much. Very clear, nice images I got every time.

Date published: 2017-06-20

Rated 5 out of
5 by
haruis from
specific bandspecific band at 43kda for WB analyisis,dilution 1:600.

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