The only live-cell superresolution microscope with resolutions down to 25 nm* (*only depending on the sample) - DyMIN STED dramatically reduces the light irradiation on your sample (up to two orders of magnitude) and features the lowest light dose of all superresolution methods.

Resolution truly down to 25 nm - As demonstrated by separating two fluorescent point-structures being 30 nm apart.

Volume / time-lapse imaging with easy3D STED resolution - DyMIN STED substancially reduces photobleaching and enables long term measurements over volumes or over dozens and dozens of frames.

The combination of the Abberior Instruments STED system with the Becker & Hickl Simple-Tau 150/154 TCSPC FLIM system records FLIM data at a spatial resolution of better than 40 nm. The image format can be as large as 2048 x 2048 pixels, with 256 time channels per pixel. An image area of 40 x 40 micrometers can thus be covered with 20 nm pixel size, fully satisfying the Nyquist criterion. The system benefits from Windows 64 bit technology used both in the Abberior and in the bh data acquisition software, from the combined processing power of the two system computers, and the high data throughput of up to four parallel TCSPC FLIM channels. The system achieves peak count rates in excess of 5 MHz per FLIM channel, resulting in unprecedented signal-to-noise ratio and short acquisition time.

3D-protected STED imaging of neuron in living brain tissue. 3D-rendering of a 3D-image stack of a living neuron expressing the AC-rsEGFP2 actin label in a hippocampal brain slice. Protected STED was applied with resolution enhancement in the xy and z directions. The image shows a stretch of dendrite with dendritic spines. The stack corresponds to 56 z slices spaced by 80 nm. Scale bar: 1 µm.

Abberior Instruments Protected STED technique has been highlighted in a new January 2016 paper in Nature Photonics. Full paper available for download here.

Abstract: By exploiting a second off state of a reversibly switchable fluorophore, a general approach that can reduce photobleaching and enhance resolution of coordinate-targeted fluorescence nanoscopy has been demonstrated.

Abberior Instruments is proud to announce its new easy3D STED module which uses a completely novel approach to 3D STED microscopy. At its core, a programmable spatial light modulator (SLM) is used to create the phase patterns required for 2D and 3D STED microscopy. At the same time, it can also be used to correct for optical aberrations.

Abberior Instruments is known for extraordinary customization ability of its flexible STED microscope platform. We now take things in a different direction. We have developed a completely new instrument, the ultimate compact STED plus confocal microscope, called STEDYCON.

STEDYCON is a completely new class of nanoscope. It converts your conventional epifluorescence microscope into a powerful multicolor confocal and STED system! At the same time, it is incredibly compact and can be used by anyone!