ELISA: In combination with anti-PRL capture antibody (Cat No: ARG10175), this HRP conjugated antibody can be used as tracer in sandwich ELISA for human PRL detection.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form

Liquid

Purification

Protein G affinity purified

Buffer

0.01M PBS (pH 7.2) and 50% Glycerol

Stabilizer

50% Glycerol

Storage instruction

For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Prolactin (PRL) is primarily secreted by pituitary gland. The hormone stimulates mammary gland development during pregnancy and lactation after childbirth. PRL has several variations with molecular weights ranged from 22kDa to 150kDa. PRL receptors are mainly located at mammary glands, but also found in other organs and tissues. PRL can bind to cytokine-like receptors and influence haematopoiesis, angiogenesis, and immuno-system. Anterior pituitary tumours can cause persistent elevation of PRL with clinical signs such as abnormal lactation, infertility and lack of menstrual flow in women, and incompetence in men.