Mutation nrdH754(del)::kan

The primers used to make this knockout were ACGCGTATCATCGCGCCGTATCTTCATTTTAAACGGAAATACGAATCATGATTCCGGGGATCCGTCGACC and GGAGCTGCTGGAGAAGTAGACGAGCTGGCTCATGCACTGGCCGCGTGTGGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template