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Investigators in Switzerland report their study of the establishment of a gut microbiome in infants in PLOS One. Using a combination of culturing, 16S rRNA gene sequencing, qPCR analysis, and Sanger sequencing, they examined the bacterial makeup of feces from seven healthy, vaginally delivered, breast-fed babies. The researchers found that there were more anaerobic bacteria than facultative anaerobic bacteria in all samples, with high levels of Bifidobacterium and Bacteroides.