• Standard Oligos

For routine applications, Bioneer offers standard custom Oligos without modification in tube format from 25 nmole scale upwards at very competitive pricing.
Bio-RP (near-HPLC) purification is included at no additional charge and these oligos can be ordered online or via e-mail from this website with simplicity.

Features and Benefits

Free Bio-RP purification: Near HPLC purity at a standard oligo price

Quick turnaround time: < 3.5 business days from order to bench in most cases

Competitive pricing: Great value for your research dollar

Quality that you can rely on

Why is Bio-RP purification better than desalting?

Most oligos sold are merely deprotected/desalted. The process of deprotection/desalting only partially purifies the oligo and leaves behind many impurities* and failure products (truncated oligos that will not amplify and that may interfere with certain applications). These can lead to artificially inflated OD readings that increase with oligo length.
Bio-RP purification removes these contaminants and failure products, resulting in an oligo that demonstrates near HPLC purity. The advantage to you is that our product contains only quality oligos minus impurities that may inhibit some PCR reactions and that potentially skew qPCR reactions.
We deliver the same amount of active oligo as our competitors; they just have more “other” artificially inflating their OD.
To demonstrate this, plates of Oligos of various length were tested for concentration in 2 steps:
1) following a deprotection/desalting step only, and 2) following Bio-RP purification. (Fig. 1)

The results of these tests show that the amount of failed sequences and impurities* increases with length (as expected), but the failure product is not removed by deprotection/desalting alone. This results in inflated OD readings for oligos depending on oligo length.
(Table 1)

Figure 1.Plates of Oligos of various length were tested for concentration following desalting and then by Bio-RP cartridge purification.
Note inflated OD readings when deprotection/desalting alone is used.

Oligo Length (# bases)

18 - 24

24 - 29

30 - 34

35 - 41

OD Inflation

38%

45%

60%

77%

Table 1.Average OD inflation for oligos of various length when protection/desalting alone is employed v. Bio-RP purified product.
Note for longer oligos; up to 77% of product does not consist of functional oligo.