Fatty acids are important for general cellular metabolism. A number of proteins have been implicated in the transport and storage of fatty acid. FABPs (fatty acid binding proteins) are a group of cytoplasmic, small mol wt (14-15kD) and proteins that has widespread tissue distribution. FABPs are quite abundant (3-5% of total cellular protein). At least 7 FABPs, FABP1-7, have been cloned and characterized from various tissues. FABPs can bind long-chain fatty acid, fatty-acid acyl-CoA and acyl-L-carnitine. Several different isoform of FABP have been identified and generally referred to by tissue type (liver, heart, intestine, adipocyte, kidney, brain etc; protein designated as H-FABP indicates that it is heart type). However, expression of these isoform is not exclusive and more than one isoform can be found in aH given cell or tissue. Three main types of FABPs that were initially discovered in the heart (FABP-), liver (FABP-L), and intestine (FABP-I) are not exclusive these tissues and show considerable differences at the amino acid level (~30% identity). Other FABPs recently detected in adipocyte, kidney, and brain show a high degree of sequence homology among each other and with other FABPs. FABPs are also known as mammary derived growth inhibitor (MDGI), adipocyte lipid binding protein (ALBP), Myelin protein P2 homolog, P2 adipocyte protein, 422 protein (P15). Human FABP-H or adipocyte is 132-aa protein (chromosome 2p11) Rat and mouse adipocyte-FABPs are 133 aa single polypeptide chains.

Catalog #

F0019-77G

Applications

Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution

Western Blot: 1:1000-1:5000. Use +ve as control (refer to Catalog F0019-77M). Load ~10ul/lane.

ELISA: 1:10,000-1:50,000.

Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.