Application Information: Cyclin B1 antibody [GNS1]

For WB: Use at a concentration of 1-2µg/ml for 2hrs at RT. Optimal dilutions/concentrations should be determined by the end user.

Positive Controls

MAD109 or LS174T cells

Concentration

0.2
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Protein G affinity purified

Specifications: Cyclin B1 antibody [GNS1]

Full Name

cyclin B1

Background

In mammals, cyclin B associates with inactive p34 cdc2 which facilitates phosphorylation of p34 cdc2 at aa 14 Thr and 15 Tyr. This maintains the inactive state until the end of G2 phase. The inactive cyclin B p34 cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34 cdc2 rendering the complex active at the G2 / M boundary. This mitotic kinase complex remains active until the metaphase / anaphase transition when cyclin B is degraded. This degradation process is ubiquitin dependent and is necessary for the cell to exit mitosis. Therefore, cyclin B p34 cdc2 plays a critical role in G2 to M transition.

Synonyms

CCNB1, Cyclin B1, 891, 123836, P14635, CCNB

Host

Mouse

Clonality

Monoclonal

Clone Name

GNS1

Isotype

IgG1

Immunogen

Recombinant protein corresponding to human Cyclin B1.

Antigen Species

Human

Species Reactivity

Human

Conjugation

Unconjugated

Storage Conditions: Cyclin B1 antibody [GNS1]

Storage Buffer

PBS, pH7.4 containing 0.2% BSA and 0.09% sodium azide

Storage Instruction

Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.

Notes

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.