First into the Tank are entrepreneurs from Arcadia and Folsom, California, who have a solution to fight back against bothersome telemarketers and scammers. Entrepreneurs from Oakland, California, present their easy-to-use 3D printer for kids to design and print their own toys, while an entrepreneur from New York, New York, pitches her savings and gifting online platform to help kids learn to save. Last into the Tank is an entrepreneur from Labelle, Missouri, who familiarizes the Sharks with her ethical and customizable meat box subscription delivery service.

Shark Tank follows aspiring entrepreneurs as they present various business ideas to moguls (known as "the sharks") in hopes of landing an investment in their ideas. Each week, ambitious entrepreneurs present their breakthrough business concepts to ruthless investors to convince them to invest in the concept.