Mouse sperm cryopreservation

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"This service provides an alternate procedure to preserve important mouse lines that either fail to generate embryos for cryopreservation, or when the breeder males are not located within the barrier Facility. It offers similar benefits to those of embryo cryopreservation by protecting the mouse lines from losses and by reducing the housing cost and cage space.
For this service we recommend a one-time harvest of embryos to be frozen prior to sacrificing the males for cryopreservation.
A set of 5-7 young male mice will be subjected to a waiting period in the containment area, as required by ULAR, prior to admission into the barrier colony. Once admitted, the animals will be mated with females to generate embryos to be frozen. Then 3 males will be sacrificed and the sperm collected and frozen."
<b>Alternate service for sperm cryopreservation</b>
"The user isolates the epididymis from 3 healthy males and brings them to the TCMF. The sperm will be isolated and frozen. The TCMF can bring back these lines subsequently via in vitro fertilization (IVF). For sperm cryopreservation at least 3-5 males between the ages of 8 weeks and 6 months are optimal. For animals that cannot be transferred to the CRB facility, the isolation must be done by the user in his/her own facility. This requires hands-on training of lab personnel for the epididymis harvest. Media will be supplied by the TCMF to all labs. Five straws of sperm per male will be frozen. For this service , we recommend a one-time harvest of embryos to be frozen prior to sacrificing the males for sperm cryopreservation."