Participants in this arm will receive a total of three doses of study vaccine: the first dose at Day 0, the second dose at Day 28 (plus or minus 7 days), and the third dose at Day 56 (plus or minus 7 days). Each dose will consist of a 1-mL injection (dose strength 4 mg/mL) and will be administered by IM injection in the deltoid muscle.

Biological: VRC-HIVDNA009-00-VP

Administered as a 4 mg/mL dose by IM injection in the deltoid muscle using the Biojector 2000 needle-free injection management system.

Placebo Comparator: Placebo vaccine

Participants in this arm will receive a total of three doses of a placebo vaccine: the first dose at Day 0, the second dose at Day 28 (plus or minus 7 days), and the third dose at Day 56 (plus or minus 7 days). Each dose will consist of a 1-mL injection and will be administered by IM injection in the deltoid muscle.

Biological: Placebo vaccine

Administered as a 1-mL dose by IM injection in the deltoid muscle using the Biojector 2000 needle-free injection management system.

Detailed Description:

The global HIV/AIDS epidemic continues to increase at unacceptably high levels, and there is not yet an effective prevention method for HIV infection. This study will test the safety and immune responses of a preventive HIV vaccine, VRC-HIVDNA009-00-VP, which is a multiclade HIV-1 DNA plasmid vaccine.

This study will seek to enroll 30 participants, randomly assigning 15 participants each to the study vaccine arm and the placebo vaccine arm. Three doses of the vaccine will be given by intramuscular (IM) injection using the Biojector 2000 needle-free injection management system: the first dose at Day 0 (entry), second dose at Day 28 (within 7 days before or after Day 28), and third dose at Day 56 (within 7 days before or after Day 56). Participants will attend two screening visits and visits at Days 0 (entry), 2, 14, 28, 30, 42, 56, 58, 70, 84, 168, 252, and 336. At most visits, participants will give a medical history and undergo a physical exam, blood draw, and urine collection. For women, a pregnancy test will be given at each study injection visit. Participants also will be instructed to maintain a diary of local and systemic reactions for 7 days after each injection. They also will be provided with a ruler to measure erythema, induration, or other observable reactions and a thermometer to record temperature on a daily basis.

Eligibility

Ages Eligible for Study:

18 Years to 40 Years (Adult)

Genders Eligible for Study:

Both

Accepts Healthy Volunteers:

Yes

Criteria

Inclusion criteria:

18 to 40 years old

Available for follow-up for the duration of the study (13 months)

Satisfactory completion of an Assessment of Understanding prior to enrollment defined as 90% correct with three opportunities to take test. Errors will be reviewed with volunteer after each test.

Able and willing to sign the informed consent form

Willing to receive HIV counseling and testing in accordance with Ugandan Ministry of Health Guidelines

Willing to discuss HIV infection risks and not currently at high-risk of HIV as defined by responses to a standard questionnaire found in the protocol, agree not to engage in high-risk behavior for HIV during the study as defined by protocol, and amenable to risk-reduction counseling

Willing to permit a home visit after one of two screening visits

In good general health without clinically significant medical history

Physical examination and laboratory results without clinically significant findings within 45 days prior to first injection

Negative urine or serum-human chorionic gonadotropin (HCG) pregnancy test for all women. A female participant must meet one of the following criteria from 21 days prior to vaccination until 3 months following the last vaccination:

No reproductive potential because of a hysterectomy, bilateral oophorectomy, or tubal ligation. Documentation from qualified medical doctor must be obtained for confirmation of procedure, OR

Participant agrees to consistently practice contraception at least 21 days prior to vaccination and throughout the duration of the study by one of the following methods:

condoms, male or female, with or without a spermicide

diaphragm or cervical cap with spermicide

intrauterine device

contraceptive pills, Norplant or Depo-Provera

male partner has previously undergone a vasectomy for which there is documentation

abstinence from sexual activity

Men should not take part in this study if they plan to father a child between the first vaccination and 3 months after the last vaccination. Men should consistently use a condom, provide documentation of vasectomy from qualified medical doctor, practice abstinence of sexual activity, OR his female partner should use one of the following methods:

female condoms, with or without spermicide

diaphragm or cervical cap with spermicide

intrauterine device

contraceptive pills, Norplant or Depo-Provera

Exclusion Criteria

Woman who is breast-feeding

Has received HIV vaccines in a prior clinical trial

Has received immunosuppressive or cytotoxic medications within the past 6 months with the exception of corticosteroid nasal spray for allergic rhinitis or topical corticosteroids for an acute uncomplicated dermatitis

Has received blood products within 120 days prior to HIV screening

Has received immunoglobulin within 60 days prior to HIV screening

Has received live attenuated vaccines within 30 days prior to initial study vaccine administration

Has received investigational research agents within 30 days prior to initial study vaccine administration

Has received medically indicated subunit or killed vaccines, e.g. influenza, pneumococcal, or allergy treatment with antigen injections, within 14 days of study vaccine administration

Is receiving current anti-tuberculosis (TB) prophylaxis or therapy

History of a serious adverse reactions to vaccines such as anaphylaxis, hives, respiratory difficulty, angioedema, or abdominal pain

History of autoimmune disease or immunodeficiency

History of asthma that is unstable or required emergent care, urgent care, hospitalization, or intubation during the past 2 years or that requires the use of oral or intravenous corticosteroids

History of diabetes mellitus (type I or II), with the exception of gestational diabetes

History of thyroid disease including history of thyroidectomy and diagnoses that required medication within the past 12 months

History of serious angioedema episodes within the previous 3 years or requiring medication in the previous 2 years

History of hypertension controlled by medication or is more than 140/90 at enrollment

History of bleeding disorder diagnosed by a doctor (e.g., factor deficiency, coagulopathy, or platelet disorder requiring special precautions) or significant bruising or bleeding difficulties with IM injections or blood draws

History of active syphilis documented by exam or serology unless positive serology is due to remote (greater than 6 months) treated infection or positive rapid plasma reagin (RPR)/venereal disease research laboratory (VDRL) is not associated with positive treponemal specific serology

History of malignancy that is active or treated malignancy for which there is not reasonable assurance of sustained cure or malignancy that is likely to recur during the period of the study

History of seizure disorder other than febrile seizures under the age of 2, seizures secondary to alcohol withdrawal more than 3 years ago, or a singular seizure not requiring prolonged treatment more than 3 years ago

History of asplenia or any condition resulting in the absence or removal of the spleen

History of psychiatric condition that precludes compliance with the protocol, past or present psychoses, past or present bipolar disorder or suicidal ideation

History of any medical, psychiatric, or social condition or occupational or other responsibility that, in the judgment of the investigator, would interfere with or serve as a contraindication to protocol adherence or a volunteer's ability to give informed consent

Contacts and Locations

Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study.
To learn more about this study, you or your doctor may contact the study research staff using the Contacts provided below.
For general information, see Learn About Clinical Studies.

Please refer to this study by its ClinicalTrials.gov identifier: NCT01549470

Locations

Uganda

Makerere University Walter Reed Project (MUWRP)

Kampala, Uganda

Sponsors and Collaborators

National Institute of Allergy and Infectious Diseases (NIAID)

Investigators

Study Chair:

Fred Wabwire-Mangen, MB ChB, DTM&H, MPH, PhD

Makerere Univ. Institute of Public Health, Dept. of Epidemiology and Biostatistics