Posts Tagged / protocol

Motivation: Characterizing large quantities of single cells is important to address clinically and biologically relevant questions. Traditionally, bulk measurements have been used to characterize the functional states of diseased vs. normal immune cells, livers, brains, and tissues or organs of interest to identify various interesting mechanistic differences. However, these bulk measurements average information across many

RNA-seq allows for quantiative transcriptomic profiling of biological samples. RNA-seq takes advantage of technological advancements in high-throughput sequencing by converting RNAs of interest to a library of cDNA fragments that can then be sequenced using standard DNA-sequencing technologies. Preparing a sample for RNA-seq typically begins with either rRNA depletion of mRNA enrichment. This is due

DOP-PCR = degenerate oligonucleotide priming polymerase chain reaction Method Overview: In the first cycling stage (5-8 cycles), low-temperature annealing and extension occur at many binding sites in the genome and become tagged with the DOP primer. In the second cycling stage (>25 cycles), annealing temperature is raised, increasing priming specificity during amplification of the tagged