Product Info

Homogenizer for various starting materialsThe SpeedMill PLUS is a highly efficient homogenization system for various starting materials used for the subsequent isolation and purification of DNA, RNA or proteins. The homogenization process is based on an innovative mechanical principle for which a patent has been filed. This new process allows users to operate the SpeedMill PLUS continuously if necessary. The high efficiency of energy input into the sample, based on a vertical movement, procures a homogeneous disruption of the sample without destroying the target molecules.

Efficient sample cooling: prior, during and after preparationFor the optimized sample holder, which is used inside the SpeedMill PLUS, different temperature ratings are freely selectable due to the storage down to –80 °C. According to this an efficient sample cooling during the whole homogenization process is warranted and the substantial sample warming that occurs with other homogenizers is prevented. The often problematic handling of liquid nitrogen or dry ice is thus a thing of the past. Additionally the considerably expense factor of this additives, which have to be loaded continuously, is not applicable. Besides the sample holder allows an easy transport of the sample tubes and a long term storage of starting or homogenized material at adequate temperatures.

Modern preparation of samples: SpeedMill PLUSThe samples to be processed are rapidly and efficiently homogenized in Lysis Tubes that have been specially optimized for the system and, as such, contain different and/or application-specific beads. Using beads makes it possible to completely and reproducibly homogenize even the toughest starting materials, such as cartilage and chitin shells of insects or ticks within a very short time. 2.0 mL and 0.5 mL containers (Lysis Tubes) with different beads are available for sample preparation, allowing users to adapt sample processing to a diverse range of soft and hard starting materials. Operating processes, such as loading and removing of the sample tubes, are very simple and no tools are required. In addition userdefined protocols can be entered and saved as well as pre-installed programs are available. Homogenization parameters, like time and using cyclic routines are freely selectable.

Optimized extraction kits for the SpeedMillThe SpeedMill also accommodates kits for complete nucleic acid (DNA and RNA) isolation from various starting materials. All kits have been optimized for the SpeedMill for extremely fast and efficient nucleic acid isolation. The yields produced are impressively high and the quality of the isolated nucleic acids is outstanding. These kits contain special Lysis Tubes with application-specific beads as well as pre-made buffers.

They also contain all other components needed for isolating DNA or RNA from different starting materials. Optimized kits for sample processing with the SpeedMill results in extremely rapid and highly efficient nucleic acid isolation. Both the yield and quality of the nucleic acids are excellent. The standard isolation protocol requires only about 20 to 30 minutes.

Nucleic acid extraction principleDNA isolation: Mechanical disruption of the starting material is followed by a proteolytic lysis step. The genomic DNA is adsorbed onto a Spin Filter, washed and then eluted. The yield and quality of the DNA are excellent.

RNA isolation: After the mechanical disruption and denaturation of the starting material, genomic DNA is removed by adsorbtion onto an initial Spin Filter. The RNA is then adsorped onto a second Spin Filter, followed by a wash step and finally by elution of the RNA.