Albumin is synthesized in the liver and consists of a single polypeptide chain with a molecular weight of 66kD. It is present in all body fluids and is most abundant plasma protein. It acts as a transport protein for long chain fatty acids, prostaglandins, hematin, thyroxin, copper II, cystine and pyridoxal phosphate amongst many other substances that bind more weakly. The average serum concentration of albumin is 42g/L.

Catalog #

A1327-49

Applications

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions

ELISA: 1:10,000-1:50,000

Western Blot: 1:1000-1:5000

Immunohistochemistry: 1:500-1:2500

Optimal dilutions to be determined by the researcher.

Storage and Stability

Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.