Dot blot hybridisation of RNA - (Dec/17/2008 )

Hi all....

I have labelled DIG probes(PCR product) to check the expression of three genes. Since the concentration of the probes labelled is only for 6 reactions, i planned to do dot blot of RNA. I have a problem here that is i dont have a dot or slot blot apparatus which is recommended. Which is the protocol i follow to do dot blot of RNA? Is there any protocol which says we can do dot blot without an apparatus? If there kindly share with me.

Thankyou

Enjoy Research.....

-chimmi-

It is possible to dot blot without the apparatus, it just takes about 20 times as long and is less precise. Basically, you will need to spot small volumes (probably about 1 ul at a time) of your dilutions onto a membrane and allow to dry before spotting more on. Repeat until all your dilutions are done and then proceed as normal.

-bob1-

QUOTE (bob1 @ Dec 18 2008, 06:19 AM)

It is possible to dot blot without the apparatus, it just takes about 20 times as long and is less precise. Basically, you will need to spot small volumes (probably about 1 ul at a time) of your dilutions onto a membrane and allow to dry before spotting more on. Repeat until all your dilutions are done and then proceed as normal.

Hi....

Is there a protocol handy how to do dot blot of RNA....If any please do tell me....