The effect of temperature stresses on Cefaclor suspensions under different storage conditions for a duration of 14 days was tested. The degradation of Cefaclor was determined on the 2nd , 7th and 14th day after reconstitution using a sensitive and precise Reversed phase High Performance Liquid Chromatographic (RP-HPLC) method. The RSD values for Forticef, Midocef, Ceclor, Cefabac and Cloracef, indicated a good precision of the RP- HPLC method. The limit of detection (LOD) and the limit of quantification (LOQ) were found 0.008 mg/ml and 0.03mg/ml respectively. The antimicrobial effect of Cefaclor suspension was also tested against pathogenic bacteria using the cylinder diffusion method. The RSD values range of the antimicrobial assay for all the Cefaclor compounds were 1.47-3.7%. The LOD and LOQ were 0.2mg/ml and 1mg/ml respectively. During the normal use of Ceclor, Midocef, and Forticef the loss of activity and the degradation were less than 5% on the 14th day of preservation at 4Â°C. However, the percentage of degradation for Cefabac and Cloracef on the 14th day reached 5 and 6%, respectively. Statistical multiple comparison between the effect of 4Â°C and 25Â°C indicated non significant mean differences (Pâ‰¥0.05) for Forticef, Cefabac, Ceclor and Cloraf and significant effect for Midocef (P â‰¤ 0.05). Significant effects were observed between (4Â°C and 37Â°C) and (25Â°C and 37Â°C) for all except Ceclor. Multiple comparisons between days of storage showed non significant mean difference values at 4Â°C except Cefabac. However significant results between days were found at 25Â°C and 37Â°C except for Midocef between (7th and 14th day). It was found that the pediatric suspensions of Cefaclor in the Jordanian market were stable and contained the amount of active ingredient specified by the United States pharmacopoeias specification (USP) and the British Pharmacopoeias specifications (BP).

A reverse phase high performance liquid chromatography (RP-HPLC) has been developed for the estimation of Tenatoprazole in bulk drug and pharmaceutical dosage form. The quantification was carried out using C18 column and mobile phase consisting of 10mM phosphate buffer at pH 2.4: acetonitrile...

A RP-HPLC assay method has been developed and validated for cefotaxime. An isocratic RP-HPLC was developed on a SS Wakosil II-C8 column (250 mm 4.6 mm i.d., 5 Î¼m) utilizing a mobile phase of ammonium acetate buffer (pH 6.8) and acetonitrile (85:15 v/v) with UV detection at wavelength 252 nm...

The bacterial strain DSM 9103, able to utilize EDTA as a sole sourceof carbon, nitrogen, and energy, is also capable to grow with [S,S]-ethylenediaminedisuccinate ([S,S]-EDDS), a structural isomer of EDTA.In cell-free extracts of [S,S]-EDDS-grown bacteria, [S,S]-EDDS degradation was observed in...

The present study describes in vitro antimicrobial and antioxidant activities of methanol extracts obtained from the leaves and twigs of the plant Daphne cneorum L. The antimicrobial activity of these extracts was tested against human pathogenic microorganisms using a minimum inhibitory...

Background and Aims Although several methods of sampling and storing floral nectar are available, little information exists on sampling and storing nectar from flowers with low nectar volumes. Methods for sampling and storing nectar from the flowers of species with low floral nectar volumes...

Voriconazole is a very potent antifungal agent used to treat serious fungal infections (candidiasis); it is also the therapy of choice for aspergillosis. After standard dosing, several factors affect exposure of voriconazole, resulting in large variability and demanding further elucidation of...

Alantolactone/isoalantolactone, a mixture of isomeric sesquiterpene compounds, was isolated from the roots of Inula helenium L. subsp. turcoracemosa Grierson and its amount was quantified by RP-HPLC. The structure of alantolactone/isoalantolactone was elucidated by using HPLC and lNMR data....

The present work is devoted to the study of antiradical and antimicrobial activities of phenolic compounds extracted from different grapevine varieties grown in the Bekaa plane-Lebanon. The amount of phenolic compounds in selected grape extracts was determined by the Folin-Ciocalteau method....