Yeast Two-Hybrid cDNA Library—Mate & Plate

Clontech strongly recommends the use of our “Mate & Plate” and "Normalized Mate and Plate" Libraries with the Matchmaker Gold Yeast Two-Hybrid System. These libraries provide, by far, the simplest method for yeast 2-hybrid screening, because they do not require library-scale transformations or labor-intensive library amplification. Thus, very little optimization or hands-on time is required with these libraries.

To screen, simply take a 1 ml library vial and culture overnight with a Y2H Gold clone that expresses your bait protein, and then plate on selective media. A single Mate & Plate Library aliquot is sufficient for the complete screening of more than one million independent clones.

Or Do It Yourself!

Alternatively you can use our Make your Own “Mate & Plate” Library System (Cat. No. 630490) to make your own customized library. With this system, you can generate enough 1 ml library aliquots for more than 100 screens.

Or You Can Upgrade Further with these Specialized Libraries:

Features

The simplest way to screen a yeast two-hybrid library

Highly complex, tissue-specific libraries

Applications

Yeast two-hybrid screening

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.