Simple sample preparation for the determination of vitamin D in serum by LC-MSMS

Tecan Group Ltd.

Prior to any analysis by LC-MSMS, a sample clean-up step is recommended in order to remove matrix components such as proteins, lipids, carbohydrates and salts.

The current methods for sample preparation require at least one labor-intensive and time-consuming step, such as filtration, centrifugation or solvent evaporation. In addition, these steps prevent straightforward automation of the sample preparation process, which is highly desirable in order to cope with the increasing number of vitamin D determinations and sample load experienced by many laboratories.

This application note demonstrates the suitability of the AC Extraction Plate for sample preparation prior to the determination of vitamin D in serum by LC-MSMS, along with details of the workflow and how to use the AC Extraction Plate.