Left ventricular cavity opacification can be produced by the intravenous injection of sonicated albumin (Albunex 422). A recent study suggested that sonicated albumin may be useful in dobutamine stress echocardiography, but its use in exercise echocardiography has not been reported. The purposes of the study were to assess the feasibility of using Albunex to enhance endocardial visualization and to evaluate the factors affecting Albunex kinetic in exercise echocardiography. Fifteen healthy volunteers underwent two exercise echocardiograms on the same day, with intravenous injection of Albunex during one of the studies. Two injections up to a maximum of 20 ml of Albunex per injection were given to enhance the four- and two-chamber views, both at rest and immediately after exercise. The degree of left ventricular enhancement and the percentages of endocardial visualization were measured in both resting and exercise images. Variables relating to the kinetics of Albunex, which included volume of contrast injected, transit time, heart rate, and cardiac output at the time of image acquisition, were also assessed. There was no difference in the image acquisition time between the two exercise echocardiograms. Albunex produced good left ventricular opacification both at rest and after exercise. More endocardial border was visualized with contrast injection than without (91.2% +/- 11.5% vs 85.8% +/- 14.2%, p = 0.007). The transit times and volumes of Albunex injected were significantly less after exercise than at rest. The parameters were inversely related to heart rate and cardiac output. In conclusion, intravenous injection of Albunex is a promising means to enhance left ventricular endocardial visualization in exercise echocardiography and can be readily incorporated without causing a significant delay in obtaining images after exercise. Whether its use can improve diagnostic accuracy of exercise echocardiography requires further studies.