Blood samples needed:The technique used for whole genome sequencing (WGS) requires fresh blood samples for DNA extraction. If you have a Bearded Collie who meets any of the health criteria below, please contact us for further instruction to submit a sample to the laboratory.

If you have previously submitted a dog’s sample to the study (buccal or blood) and this dog meets any of the below health criteria, you may resubmit that dog’s sample. Find the questionnaire to accompany samples at http://cgap.ucdavis.edu/CGAPAddisonsBloodCollectionForm.pdf.

Health criteria and number of dogs needed:1. Confirmed primary Addison’s casesdiagnosed by ACTH stimulation test with vet records/bloodwork results, diagnosed between 2-6 years old, no corticosteroid use at least 6 monthspriorto Addison’s diagnosis, and no other concurrent health issues including any other immune disease such as SLO, hypothyroidism, immune mediated hemolytic anemia, etc. Number needed - 10

2. Healthy Bearded Collies over the age of 10 yearswith no health issues either confirmed or suspected. These healthy dogs will serve as controls for both SLO and Addison’s cases. Number needed – 10 at least

3. Confirmed SLO casesdiagnosed by a veterinarian based on clinical findings and/or biopsy (with vet records) and no other health issues either confirmed or suspected, including any other immune disease such as Addison’s, hypothyroidism, immune mediated hemolytic anemia, etc. Number needed – as many as possible

Other Criteria. From the US. Reason is to insure rapid handling of blood samples and shipment.

Sample collection and shipment:

1. Shipment of samples will beprepaidby the research laboratory. Contact research assistant, Janelle Belangerjmbelanger@ucdavis.edu,for instructions on sample collection, health questionnaires and FedEx shipping account information. Note: We are unable to reimburse for costs associated with the blood draw and shipping paid for by the owner or veterinarian cannot be reimbursed.

a. Sample Collection:If the blood sample cannot be shipped right away (within 1-2 days), it should remain I the refrigerator, not the freezer until shipment. We need 2-3cc (ml) of blood minimum (purple top EDTA tube). The blood sample tube(s) should be labeled with the dog’s name, owner last name and date, and shipped on ice packs (a box with a Styrofoam insert works well). Please note that the UC Davis campus does not receive deliveries on weekends or holidays. When the sample is ready for shipment, please send the FedEx tracking # tojmbelanger@ucdavis.edu

b. Shipping Address:

Oberbauer Laboratory

Attn: Janelle Belanger

Canine Genetic Analysis Project

Department of Animal Science

University of California

2251 Meyer Hall

One Shields Avenue

Davis, CA 95616-8521

Phone # (required by FedEx): 530-752-1046

2. Blood draw expenses.

If your veterinarian charges for this and you want assistance, BeaCon will reimburse your cost. Arrangements have been made for a third party to manage the payments to keep full confidentiality of study participants.

Obtain a copy of the bill that shows itemized cost for blood draw.

Write on the bill that the charge is for Addison’s/SLO research at UC Davis

Email a scanned copy of the bill or snail mail the hard copy bill to:

Susan Harris

email: samtrack@bellsouth.net

Snail mail: 319 Harris Drive, McDonough, GA 30252

Clinical Research

The role that environmental factors, heredity, and use of preventives for fleas, ticks, or heartworm might have on the development of SLO is not known. BeaCon initiated work on SLO 2008 which has continued to the present.

More participants are needed in the clinical research; we ask you to consider donating your time and dog’s history for the cause. Before starting the survey you should gather the dog’s vaccination record, health history, use of preventives (tick & flea) and registered names of sire and dam. Once you click the done button at the end of the survey, you cannot return.