As part of a purification protocol, I have been precipitating nucleic
acids from recombinant E.coli lysates with polyethylenamine. (Add
polyethylenamine to 0.5%, incubate on ice for 20 min, spin 20,000 x g for
20 min and collect sup).
This purification protocol has not worked, so I would like to try a more
traditional purification method such as Ni-NTA and/or anion exchange
chromatography.
Any opinions as to whether this polyethylenamine treatment will affect
either type of chromatography??
thanks,
jake