Lymphoblastoid cell lines infected with Theileria parva schizonts were established from cattle in Central Province of Zambia. The parasites were genetically and antigenically different from buffalo-derived T.parva (T.parva lawrencei) and also from cattle-derived parasites (T.parva parva) in Eastern Province of Zambia, as revealed by Southern blot analysis and indirect fluorescent assay by using a panel of anti-schizont monoclonal antibodies. Polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) analysis of major piroplasm and shizont protein genes of Zambian isolates also confirmed the above-mentioned results.DNA samples were prepared from Theileria-infected African buffalo in South Africa and analyzed for the detection of Theileria by PCR based on small subunit ribosomal RNA (srRNA) gemes. They contained several species of Theileria, including T.buffeli (or sergenti) and other parasites unique in this animal species. Possible transmission of the former sp
… Moreecies among cattle and buffaloes are suggested.A major piroplasm surface antigen gene of Theileria parasite isolated from a sable antelope was amplified by PCR and its nucleotide sequence was determined. The parasite was phylogenically distinct from other Theileria species isolated from cattle and African buffalo.Babesia-like piroplasms were detected in erythrocytes of zebra in South Africa, and successfully cultured in vitro. They were morphologically similar to Babesia equi in horses. Further molecular characterizations of the zebra-derived parasites are now underway based on EMA-1 gene analysis.Several other piroplasms (Babesia or Theileria) were also detected in blood smears from other wild animal species including kudu, cheetah, giraffe, lion.caracol (wild cat in Africa). Some of the parasites have not been classified so that further molecular characterizations of the parasites based on srRNA genes are essential to clarify taxonomical and phylogenical relationships among parasites from wld animals and those from domestic animals.(2)その他の動物における原虫感染状況の調査タイレリア感染の見られたセ-ブルアンテロープから樹立されたシゾント感染細胞試料からDNAを得て、遺伝子解析を行った。本種は既にrRNA遺伝子塩基配列から固有種と考えられているが,今回の主要ピロプラズマ表面蛋白質(p32相同分子)の遺伝子解析の結果からも、既知の牛由来タイレリア種とは全く独立した位置づけにあることが確認された(角田ら,印刷中)。猫科動物(ライオン、チ-タ、カラコル[野生猫の一種])の末梢血液の塗抹標本においてタイレリアもしくはバベシアと考えられる原虫を検出した。これらの多くは未記載、未分類の原虫であり、さらにrRNA遺伝子解析を継続して行い、既知の種との遺伝的関係を明らかにする必要がある。馬科動物においては、シマウマ末梢血液中にバベシア原虫を検出し、5株についてはその試験管内培養に成功した。これらは、形態学的にウマ由来のBabesia equiと同一原虫種と考えられた。南アのウマ由来Babesia equiについては、主要ピロプラズマ表面蛋白質(EMA-1)遺伝子の解析から、少なくとも4つの遺伝型が存在することが今回の調査で明らかになった。(須藤ら、印刷中)。今調査研究内では、馬由来株との遺伝学的な比較をさらに行うには至らなかったが、野生動物が共存する地域でのウマピロプラズマ感染症の疫学を明らかにする上で、EMA-1遺伝子解析をさらに行う必要がある。 Less