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The success of siRNA technology is dependent not only on the effectiveness of the designed siRNA, but also on the efficiency of the transfection agent used to deliver the siRNA. Because transfection ... read more

A common method used in almost every lab these days is transient transfection of cell lines followed by some form of output (Western blot, reporter gene assays, etc.). In a majority of cases, one ... read more

Lipofectamine™ 2000 Reagent is a lipid-derived reagent designed to deliver exogenic nucleic acids (DNA and/or RNA) into eukaryotic cells, either adherent or in suspension. Thee two most important ... read more

Lipofectamine™ 2000 is a lipid based transfection reagent for eukaryotic cells. It can be used for transfection of adherent or suspension cells with DNA or RNA (including Stealth RNAi and siRNA) and ... read more

Transfection reagents are big business. The ability to express exogenous genes within eukaryotic cells is very popular and companies are falling over themselves marketing these products, claiming to ... read more

Invitrogen’s Lipofectamine 2000 is one of many cationic lipid-based transfection reagents presently on the market. Lipofectamine is formulated to give high transfection efficiency with little toxicity... read more

Lipofectamine 2000 Reagent is a cationic lipid-based transfection reagent designed to give extremely high transfection efficiencies and result in equally high protein expression levels. It has also ... read more

The TransMessenger Transfection Reagent from Qiagen is designed for the efficient transfection of eukaryotic cells with RNA (including siRNA). The reagent works using principles similar to the ... read more

TransMessenger Transfection Reagent is a lipid formulation-based transfection method for getting small-interfering RNAs (siRNAs) into cells in culture. The Transmessenger system is used with an RNA ... read more

RNA interference (RNAi) is a process in which double-stranded short RNA fragments inhibit the expression of genes with complementary nucleotide sequences. RNAi was first discovered in plants as a ... read more

One way for researchers to deduce a gene products’ function is to eliminate that gene, hence eliminating the protein, in an organism and observe the resulting phenotype. Traditionally this has been a... read more

RNA interference (RNAi) is a promising new gene silencing technology of great use in studying the functions of genes and understanding the genetic basis of cellular physiology in mammals. This ... read more

In our laboratory, we were looking for reliable reagents for Treg identification so that we could monitor these cells in a number of different experimental settings. So when we found the Human ... read more

The regulatory T cell staining kits from eBioscience are designed to identify the subpopulation of T cells called T regulatory cells. These cells are characterized by expression of CD4 and high levels... read more

Measuring the concentration of total interferon-alpha secreted by cells in peripheral blood samples, serum samples or even culture supernatants is not an easy task. There are 14 different isoforms of ... read more

The eBioscience IL-6 Ready-SET-Go! ELISA kit offers an easy and sensitive method to quantify IL-6 levels in biological samples. This is a ready-to-use kit which contains all of the required chemicals ... read more