Interpretive Summary: Vitamin B6 is a water-soluble vitamin and is part of the vitamin B complex group. Several forms of the vitamin are known, but pyridoxal phosphate (PLP) is the active form and is a cofactor in many reactions of amino acid metabolism, including transamination, deamination, and decarboxylation. PLP also is necessary for the enzymatic reaction governing the release of glucose from glycogen. Low vitamin B6 status has been linked to an increased risk of cardiovascular diseases. Our objective was to examine the association of vitamin B-6 status with markers of inflammation and oxidative stress. We used for this purpose Puerto Rican adults who were living in Massachusetts (n = 1205).strong dose-response relation of plasma PLP concentration. Our data show that low vitamin B6 concentrations are associated with inflammation, higher oxidative stress, and metabolic conditions in older Puerto Rican adults. Therefore, we conclude that vitamin B6 may influence cardiovascular disease risk and support the notion that nutritional status may influence the health disparities present in this population.