A Day in the life of an Embryologist

A day in the life of an EmbryologistSaiprasad Gundeti, Senior Embryologist, Malpani Infertility Clinic Pvt. Ltd.

As an embryologist, I help in making the dreams of infertile couples come true ! Most couples do not know what happens in an IVF lab, so I am happy to describe a day in my life !This is the daily routine we follow at our centre :

First thing in the morning -

Cleaning

Cleanliness is a very important factor in IVF Lab. We need to make sure everything is sterile ! Because I work alone in our lab, it's much easier for me to ensure that everything is clean !

Hood and bench work surfaces (including microscope working areas, heat baths, petridish warmer) are cleaned and wiped down with 6% Hydrogen Peroxide.

Once a week centrifuge rotors and carriers as well as outside area of the centrifuge are cleaned and disinfected using 6 % Hydrogen peroxide

Floor is nicely cleaned with 6 % Hydrogen Peroxide.

Monitoring and Maintenance of All Equipments

Your embryos can be quite demanding, and we need to keep them happy in the IVF lab ! We do this by ensuring that the conditions in the lab are just right for your embryos !

The Incubator CO2 % is confirmed with the help of CO2 analyzer. Calibrated if any anomaly seen in the readings. Temperature is measured with the help of digital thermometer. Calibration is done in case of any temperature variation seen.

Temperature is measured for all heating baths, heating plates of all microscopes and petridish warmer.

Gas cylinders connected to CO2 incubators are checked. Cylinders are changed if they are found half empty.

Monitoring of embryo development

Fertilization assessment of each patients eggs on which ICSI was performed a day before.

Stripping (denudation) of eggs on which IVF was preferred.

Transferring fertilized eggs to new dishes (prepared, labeled with patients name and incubated a day before)

Assessment of embryo development grading of day2, day3, day4, day 5, day 6 embryos.

We routinely give all our patients photos of their embryos. We take pride on our work, and good quality embryos are a testimony to the fact that we are providing a high quality service to our patients !

As soon as egg (Oocyte Cumulus Complex) is located I take clean follicular fluid in to Pasteur pipette and pour 2-3 drops over OCC and then take OCC into the Pasteur pipette and deliver in the dish at 12 O'clock position.

Inform the clinician by loudly saying "EGG" when the egg is identified.

I inform Dr. Anjali about presence of egg in the tube by loudly saying "EGG".

If there is no egg in the tube I inform by loudly saying "NOTHING" and I do inform about the presence of Granulosa cells in the fluid.

Similarly I identify and collect all the eggs at 12 O' clock position in 60mm petridish.

I Take out OPU dish labeled with ( Patient Name) and ( Egg Wash)-prepared one day earlier.

I Rinse all the OCC's in the outer well containing Flushing medium and transfer them in to the centre well of the same dish containing Fertilisation medium

Following OPU, OCC's are cultured in fertilisation medium in CO2 incubator till IVF or ICSI being done on them.

Continuing medical education

IVF is a field in which technological advances occur very rapidly. I read the journals, Human Reproduction and Fertility Sterility, to ensure that I am uptodate !

Preparation for the Next Day

I go through the files of all patients that are scheduled for next day.

Preparation depends on what procedure patient is undergoing.

Fertilisation check dishes:dishes for each patient

Dishes / medium for OVUM PICK UP :

1 tube with Oil (6ml)1 tube with Flushing medium (6ml)

Dishes for IVF (No. of Dishes dependent on no. of follicles):

Dishes for ICSI (3 Dishes for each patient):

Dishes for Frozen Thaw (2 Dishes for each patient) :

Medium for Embryo Transfer :

1 ml for each patient

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