Note: Tractography shown in this protocol was performed in DTIStudio using 30 direction diffusion data.

To identify the IFO:

First ROI: Identify the coronal slice in which the green fibers connecting the temporal and parietal lobes are most intense (marked by arrows; 1). This slice is typically a few slices anterior to the splenium of the corpus collosum. Select these fibers (2).

1

2

Second ROI: Identify the coronal slice in which the tract bunches, typically at the anterior portion of the fornix. Select the circular cluster of fibers running through the corticospinal tract.

* Be sure to remove all fibers that are clearly not a part of the tract. Note the inferior longitudinal fasciculus, which has fibers beginning in the temporal lobe and projects similarly to the IFO.