Colleagues and I have previously identified a panel of candidate genes involved in influencing egg size in fruit flies (Jha et al. 2015). I have derived genetically stable inbred fly lines from flies artificially selected for large eggs, as well as those adapted to long term cold temperatures that also have large eggs. I intend to use Next-Generation Sequencing to 1) to begin to validate candidate genes and 2) identify differences between lines produced in a period of months by direct selection and lines produced over a period of years by environmental selection. Do directly selected flies utilize the same pathways to build large eggs as those generated indirectly? I will also continue to phenotype life-history traits in these lines in an effort to identify tradeoffs that may differ between directly and indirectly selected lines. These data may allow us to begin to assemble genetic networks for the trait.