An endangered tiger quoll has been filmed in the Grampians National Park in Victoria just weeks after bushfires devastated a third of the park.

Until last year, tiger quolls were thought to have been locally extinct as the animal had not been seen in the Grampians area in more than 140 years.

The nocturnal creature was unexpectedly captured on film by remote digital cameras on February 3, following the first sighting in September last year.

While park rangers do not know if it is a the same animal, the fact that the species was still calling the area home was a great thrill.

Parks Victoria chief executive Bill Jackson said staff were extremely worried about the impact the blaze might have had on threatened wildlife.

The fire came within two to three kilometres of the area where the endangered spotted quoll was first filmed and where a precious population of threatened brush-tailed rock wallabies live.

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There were also fires in the park last year. Over the past decade, most of the Grampians has been burnt at some stage.

Dr Jackson said that, even if it was the same animal, the sustained presence of the tiger quoll in the ecosystem signalled all was well.

"The fact that it's still there tells you that it is able to survive and feed itself and, from the footage, it looks to be healthy," he said.

Grampians National Park ranger in charge Dave Roberts said that, given the elusive nature of the tiger quoll, parks staff were on the look-out for droppings, or scats, which would shed light on the animal's diet. Fur samples would also allow for genetic sampling.

"That could tell us if it was a Victorian animal or if it had come from somewhere else," he said.

Sitting at the top of the food chain, tiger quolls are top order predators in the area – a local equivalent of the Tasmanian devil.

Tiger quoll numbers suffered following the introduction of foxes and cats and were hunted by pastoralists who were protecting their stock.

However, the sightings may indicate that the decade-old predator program targeting foxes and cats, initially introduced to benefit native mammals, may also be aiding the return of the tiger quoll to the area.

The last confirmed sighting of a tiger quoll in the Grampians area was in 1872, when the creature was considered by pastoralists to be a pest. That animal was killed at the headwaters of the Glenelg River.