Don married the girl he was in love with in high school, Melvamay Kolokea Wong, on November 21, 1951. She was the mother to the first six of his children. Ho was married for 48 years until she died on June 8, 1999. While in the military, Ho lived in many states with his family until he was called home to help his mother Honey Ho, with the family bar business called Honey's.

Ho had ten children. His children often worked with him either. They either performed with him or were a part of the business part of his performances. He loved to work with his children. He taught them the entertainment business.[3] His daughter, Hoku, performed with her father in his Waikīkī show. In 2000, she went on to become a well known singer. In 2005, he sang a song that was used as the opening theme to the direct-to-video and DVDmovieAloha, Scooby-Doo!.

In 1995 at the age of 65, Ho's health began to get bad. He had a small stroke. By 2002, he had a heart condition that could not be fixed. He was always hopeful no matter how bad his health was. He always felt there was a way to fix things. He also kept singing ever night.

Around September 12, 2006, Ho married Haumea Hebenstreit. She worked on his show in Waikīkī.[6] A few days later Ho went into cardiac arrest. He had had a new pacemaker installed on September 16, 2006,[7] but still died in Waikīkī from heart failure on April 14, 2007.