DSpace Community:http://dr.lib.sjp.ac.lk/handle/123456789/181
Fri, 02 Mar 2018 12:58:02 GMT2018-03-02T12:58:02ZNovel member of Ras family proteins from Disk Abalone (Haliotis discus discus): Structural profiling and its transcriptional modulation under host pathologic conditionshttp://dr.lib.sjp.ac.lk/handle/123456789/6936
Title: Novel member of Ras family proteins from Disk Abalone (Haliotis discus discus): Structural profiling and its transcriptional modulation under host pathologic conditions
Authors: Elvitigala, D. A. S.; Premachandra, H. K. A; Wan, Q.; Lim, B.; Jung, H.; Choi, C. Y.; Lee, J.
Abstract: Among small GTPases, the Ras family proteins capture a remarkable place in dictating
cellular proliferation, differentiation and survival in development of an organism. Major
members of the Ras family include Ras (H-Ras, K-Ras, N-Ras), Rap1, and Rap2, all of
which can act as oncogenes upon mutation. In the present study, a novel Ras family
protein (AbRFP) was characterized from Disk Abalone (Haliotis discus discus), an
economically important, edible marine gastropod; further analyzing its transcriptional
profile in healthy and immune-challenged animals. The full-length cDNA of AbRFP is
2704 bp and it consists of an open reading frame of 552 bp, encoding a 184 amino acid
peptide with a calculated molecular mass of ~21 kDa and isoelectric point of 8.63. The
amino acid sequence resembles the characteristic features of typical Ras family proteins,
including GTP/Mg2+ binding sites and guanine nucleotide exchange factor (GEF)
interaction sites, as predicted by the NCBI-conserved domain database server.
Phylogenetic study of AbRFP showed the generally accepted relationships, with AbRFP
exhibiting highest proximity to a Ras protein from Portuguese oyster. Quantitative realtime
PCR detected ubiquitous AbRFP mRNA expression, with strongest levels in muscle
along with mantle and the lowest level in hepatopancreas. The AbRFP transcriptional
profile in gills of Abalone challenged with Vibrio parahaemolyticus or viral hemorrhagic
septicemia virus (VHSV) demonstrated significant up-regulations (p < 0.05) at 12 h and
24 h post-injection (p.i.), respectively. Moreover, significant elevation (p < 0.05) of
mRNA expression was detected in hemocytes at 72 h p.i. with V. parahaemolyticus.
These findings suggest that AbRFP may play a role under pathological conditions in Disk
Abalone.Fri, 01 Jan 2016 00:00:00 GMThttp://dr.lib.sjp.ac.lk/handle/123456789/69362016-01-01T00:00:00ZTowards a Steady State Economy in Sri Lankahttp://dr.lib.sjp.ac.lk/handle/123456789/6935
Title: Towards a Steady State Economy in Sri Lanka
Authors: Wijeratne, A. T.; Dias, P.; Adhikari, N.
Abstract: In general, it is desired that Sri Lankan economy shows growth. A growing economy
brings waste production which leads environmental pollutions such as air pollution water
pollution etc. At present, increasing population in Sri Lanka requires more natural
resources to meet the market demand. The ultimate result is an imbalance in the
biological cycles, and an irreversible change in both economic process and environment.
An irreversible economic process increases entropy. Ultimately, the entropy will reach its
maximum value. Then everything will become standstill since there would not exist more
energy to continue the economic process. As a solution, the concept of a steady state
economy is structured.Sri Lankan economy was assessed within steady state economics
to evaluate the present economic situation of Sri Lanka. A statistical analysis was carried
out on Gross Domestic Product (GDP), population, energy use, CO2 emission through
time series analysis and regression analysis,to identify the extent to which Sri Lankan
economy has deviated from a steady state economy. Regression analysis indicates a
strong relationship between GDP and CO2 emission. Total population size in Sri Lanka is increased from 9.9 million in 1960 to 20.48 million in 2013. CO2 emission per capita is
increased from 0.25 metric tons in 1960 to 0.65 metric tons in 2010. CO2 emission is
increased from 2259 kiloton in 1960 to 12831 kiloton in 2010. Rapid growth rates, CO2 emissions, population growth rates reveal that Sri Lankan
economy is far apart from the concept of steady state.Transition to a steady state
economy would require the implementation of new policies to restrict the utilization of
nonrenewable resources. On the other hand it is mandatory to have legal regulations
encouraging renewable resource use, energy efficiency, and reuse and recycling.Fri, 01 Jan 2016 00:00:00 GMThttp://dr.lib.sjp.ac.lk/handle/123456789/69352016-01-01T00:00:00ZNucleotide Sequence and Secondary Structure Variations in ITS2-rDNA Region of the Members of Anopheles culicifacies (Diptera: Culicidae) Species Complexhttp://dr.lib.sjp.ac.lk/handle/123456789/6934
Title: Nucleotide Sequence and Secondary Structure Variations in ITS2-rDNA Region of the Members of Anopheles culicifacies (Diptera: Culicidae) Species Complex
Authors: Shanika, K.H.S.; Harischandra, I.N.; De Silva, B.G.D.N.K.
Abstract: Anopheles culicifacies, major vector of malaria in Sri Lanka is a five member species
complex. Differences of the vector competence of siblings, is still poorly delineated.
Therefore, the current study was carried out to observe any relationship of the variations
in vector competence to ITS2 nucleotide sequences and secondary structure
characteristics of the species complex. DNA was extracted from sibling species B and E,
the ITS2 region was amplified and sequenced. Sequences for A, C and D siblings were
retrieved from NCBI GenBank. The complex divided into two groups, AD and BCE
based on primary and secondary structure of the ITS2 sequences. Secondary structures of
all species had three helices where pyrimidine-pyrimidine mismatch in Helix II and a
UUUGG motif at 5‘ of Helix III were displayed only for B, C and E. Among five types of
loops, interior and exterior loops were more conserved than other loop types. Results
showed the major vector sibling E and poor or non vector sibling B shares identical
nucleotide sequence and secondary structure. Therefore, ITS2 secondary structure is
independent of the vector competence of the sibling species.Fri, 01 Jan 2016 00:00:00 GMThttp://dr.lib.sjp.ac.lk/handle/123456789/69342016-01-01T00:00:00ZAndroid Application: Sound Pressure Level Meter and Frequency Spectrum Analyzerhttp://dr.lib.sjp.ac.lk/handle/123456789/6933
Title: Android Application: Sound Pressure Level Meter and Frequency Spectrum Analyzer
Authors: Bandara, N. D. N. C.; Wanniarachchi, W. K. I. L.
Abstract: In this paper we present the development of Sound Pressure Level Meter and Frequency
Spectrum Analyzer application for Android based devices. The developed application can
visualize real time waveform and frequency spectrum of an input audio signal in both
linear and dB scale. The GPS location, current date and time are also embedded to the
application for convenience. A comparison was carried out with currently available
smartphone apps of similar type and the results are in good agreement with the app
developed in this project. The current version of the app is capable of capturing signal of
maximum frequency up to 4000Hz. Therefore, analysis of frequency spectrum of the
voice transmission which range between 300Hz to 3400Hz can be carried out
successfully.Fri, 01 Jan 2016 00:00:00 GMThttp://dr.lib.sjp.ac.lk/handle/123456789/69332016-01-01T00:00:00Z