Here we construct a signal transduction-like system through an approach of DNA nano-engineering. <br>

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本システムの実現により入力のタイミングが制御可能かつ一度の入力で大量の薬剤を出力できるＤＤＳの創出等への応用が期待できる。</br>

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In our project, trigger DNAs and payload molecules are encapsulated in a liposome. The trigger DNA is specially designed to collapse the liposome when some initiation stimulus is applied. Once one of the liposomes collapsed, the trigger DNAs are released from the liposome and that break other liposomes in chain-reactive fashion. This mechanism of “chain-reactive burst” enables us to release a large amount of payload molecules all at once, driven by a faint trigger signal. <br>

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Our project aims at the construction of chain of molecules-releasing system by the trigger DNA input. They are problems in previous DDS(Drag Delivery System) that the control of timing releases trigger is difficult and only constant output can get from one time input.</br>

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We believe this system offers a fundamental technique of signal transduction/amplification of various kinds of chemical or physical signals, applicable to Drug Delivery System, molecular computers and so on.<br>

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So, we created 2 systems to overcome these problems. The one is that releases trigger DNA on a certain condition. The other one is that increasing output by chain reaction of destroying liposome which starts from release of trigger.</br>

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At the first system, we make alginate hydrogel membrane including trigger, then voluntary dissolution of hydrogel and release of trigger happens by increasing temperature. At the second system, we destroy liposome by hybridizing trigger DNA to the surface of liposome. The liposome which is destroyed by trigger includes new trigger DNA so released trigger destroys neighboring liposome as chain reaction.</br>

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By creating these systems, it is expectable that creation of DDS which can control the timing of input and get a lot of output with one time input as application.</br>

Current revision

Abstruct

We are aiming at constructing a chain-reactive molecule-releasing system in this project.
In living organisms, signal transduction is widely used in many situations.
Here we construct a signal transduction-like system through an approach of DNA nano-engineering.
In our project, trigger DNAs and payload molecules are encapsulated in a liposome. The trigger DNA is specially designed to collapse the liposome when some initiation stimulus is applied. Once one of the liposomes collapsed, the trigger DNAs are released from the liposome and that break other liposomes in chain-reactive fashion. This mechanism of “chain-reactive burst” enables us to release a large amount of payload molecules all at once, driven by a faint trigger signal.
We believe this system offers a fundamental technique of signal transduction/amplification of various kinds of chemical or physical signals, applicable to Drug Delivery System, molecular computers and so on.