Figure 6.

Screen revealed RNA interference samples enriched in telophase- and metaphase-stage
nuclei. (a) Composite images of handpicked Drosophila Kc167 nuclei that were measured to guide feature selection and provide the starting
point for developing the gates. Other nuclei at the edges of each sub-panel of the
composite image were excluded from analysis. (b) A random sample of automatically
scored nuclei from the cell microarrays. The scored cell is in the center of each
panel unless the cell was near the edge of the image. Scale bars = 5 μm. (c) Genes
whose dsRNAs significantly increase the proportion of cells in telophase (first four
rows) or metaphase (last row). We excluded one gene (CG3245) that originally scored
as a telophase hit because visual examination revealed that one image contained debris
that disrupted proper analysis. Note that CG8878 should not be considered telophase-specific
(see text). The fourth column shows the fold-change in the percentage of cells with
each DNA content (2N, 4N, or 8N) and the fold-change in the total cell count overall,
with significant differences from all genes tested on the array marked with a star.
See Materials and Methods section for details on this image-based analysis of the
cell cycle distribution. The fifth column indicates the fold-enrichment for the percentage
of cells that were phospho-histone H3-positive, using the mean intensity of staining
in the nucleus, based on the average of two replicates. References cited in the last
column: A [17], B [42], C [43], D [44], E [45], F [20], G [36].