SensiFAST™ Real-Time PCR Kits

Probe

SensiFAST™ Probe has been developed for fast qPCR and is designed for superior sensitivity and specificity with probe-detection technology, including TaqMan®, molecular beacon and Scorpions® probes. A combination of the latest advances in buffer chemistry and PCR enhancers, in conjunction with an antibody-mediated polymerase promote rapid, consistently accurate detection of DNA and RNA targets from a broad range of sample types.

Products

SensiFAST Products Customer Review

Download the SensiFAST qPCR Guide with detailed product descriptions and performance data to help you choose the best product for your research

“I performed a comparison of Bioline's SensiFAST™ Probe against several other qPCR master mixes. The Bioline product performed the best overall in terms of efficiency, sensitivity and lower cost. I have also been very impressed with Bioline's customer service.”

Tiffany Kosch, James Cook University, Townsville, Australia

Probe One-Step

The SensiFAST™ Probe One-Step Kits have been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube and uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR. This also gives SensiFAST Probe One-Step unbeatable efficiency in multiplexing. SensiFAST Probe One-Step Kits consists of a 2x SensiFAST Probe One-Step mix, separate reverse transcriptase and RiboSafe RNase Inhibitor.

Select the best reagent for your research based on your instrument and sample requirements

“Very good linearity right down to 10 copies, very good correlation coefficient, good qPCR reaction efficiency and gave a single band on an agarose gel.”

King’s College London, UK

SYBR®

The SensiFAST™ SYBR® Kit has been developed for fast, highly-sensitive and reproducible qPCR and has been validated on commonly-used real-time PCR instruments. The use of an antibody-mediated hot-start DNA polymerase minimizes amplification from primer-dimers, thereby improving assay specificity and sensitivity.

Products

Select the best reagent for your research based on your instrument and sample requirements

“SensiFAST SYBR No-ROX Kit is a wonderful product, it makes all the difference between a successful and an unsuccessful experiment.”

Alcorn State University, USA

Genotyping

The SensiFAST™ Genotyping Kit has been developed for high-performance dual-labelled probe assays that deliver fast, precise and highly reproducible characterization of sequence variants, including loci with type IV SNPs. The optimized buffer system promotes clear allelic discrimination and outstanding allele clustering.

Clear discrimination of all alleles

Tight fluorescence clusters

Reproducible results in as little as 30 minutes

Reliable genotyping from even very limiting amounts of sample

Genotyping from a wide range of samples

SensiFAST™ HRM Kit is a post-PCR analysis method of characterizing nucleic acid samples based on their DNA strand dissociation behavior, with increasing temperature, in the presence of saturating fluorescent dyes. Previously unknown and even complex sequence variations can be easily detected.

Clear discrimination of even the most challenging sequence differences

Unparalleled consistency between replicate difference plots

Efficient detection from limiting amounts of sample

Reliable characterization of a broad range of sequence differences

Rapid PCR amplification for increased throughput

Products

“In the course of optimizing real-time PCR for analysis of various animal tissues, I tested multiple real-time master mixes of different brands. The SensiFAST™ SYBR No-ROX Kit produced the most valid amplification result by improving efficiency and reproducibility. With this product, Bioline offers an optimal cost-performance ratio.”

Department of Animal Nutrition, TU Munich, Germany

cDNA Synthesis

For 2-step RT-qPCR, Bioline has developed the SensiFAST™ cDNA Synthesis Kit to complement the SensiFAST Probe and SYBR® Kits. The unique combination of our high-performance reverse transcriptase and optimized TransAmp™ buffer system delivers excellent reverse transcription efficiency across a very broad range of input RNA concentrations.

SensiFAST cDNA Synthesis Kit Customer Review

SensiFAST cDNA Synthesis Kit Customer Review

“I have been using a Bioline's qPCR mix (SensiFAST SYBR No-ROX, BIO-98020) for four years with Arabidopsis cDNA on a Bio-Rad CFX-96 and a Roche LightCycler-96 in 15 or 20 ul reaction volumes. I can highlight excellent technical replication, low baseline noise and good signal/noise ratio. Reasonable price quotations allow me also to stretch my budget. I know then that with an appropriate experimental design I can trust my results and expect them on time!”

R. Alvarez, University of Essex, UK

Reviews

"I have been using a Bioline's qPCR mix (SensiFAST SYBR No-ROX, BIO-98020) ..."

R. Alvarez, University of Essex, UK

"Very good linearity right down to 10 copies, very good correlation coeffic ..."

King's College London, UK

"Great product, worked exactly how expected, and sensitive to small amounts ..."

University of South Carolina, USA

"SensiFAST SYBR No-ROX Kit is a wonderful product, it makes all the differe ..."

Alcorn State University, USA

"In the course of optimizing real-time PCR for analysis of various animal t ..."