FAQ: How does the level of star activity of KpnI-HF compare to KpnI?

The Fidelity Index which measures the minimum units to produce star activity was determined using the following conditions; 1X NEBuffer, 5% glycerol, lambda DNA (1µg/50 µl), incubated for 1 hour at 37˚C . The minimum number of units of KpnI-HF required to produce star activity in the recommended NEBuffer 4 is (≥250 units). The minimum number of units of KpnI required to produce star activity in the recommended NEBuffer 1 is (16 units).The minimum number of units of KpnI required to produce star activity in NEBuffer 4 is (4 units).