Aim: The aim of this study was to measure the effect of 3 types of flexibility training, hold-relax (HR, N.=9), active isolated stretching (AIS, N.=9) and passive static stretching (PSS, N.=11) on passive and active range of motion of hip flexion after 9 weeks of flexibility training.Methods: The subjects were physical education college students (14 men and 15 women) whose ages ranged between 20 and 24 years (age mean 21.79, SD: 2.45). A multigroup pre- post-test design with three experimental groups was used. The range of movement was measured by digitalization of pre-test and post-test images.Results: Significant differences were found among the pre- and post-test for the three groups (P<0.05) on passive range of motion of the hip flexion, although there were no significant post-test differences between experimental groups. However, on the active range of motion of hip flexion there was only significant improvement among the pre-test and post-tests for PSS group (P<0.05).Conclusion: The results suggest that PSS is the best option for the improvement of both active and passive range of motion.