Abstract

Large-scale in vivo neuroimaging datasets offer new possibilities for reliable, well-powered measures of interregional structural differences and biomarkers of pathological changes in a wide variety of neurological and psychiatric diseases. However, so far studies have been structurally and functionally imprecise, being unable to relate pathological changes to specific cortical layers or neurobiological processes. We developed artificial neural networks to segment cortical and laminar surfaces in the BigBrain, a 3D histological model of the human brain. We sought to test whether previously-reported thickness gradients, as measured by MRI, in sensory and motor processing cortices, were present in a histological atlas of cortical thickness, and which cortical layers were contributing to these gradients. Identifying common gradients of cortical organisation enables us to meaningfully relate microstructural, macrostructural and functional cortical parameters.

Analysis of thickness gradients across sensory cortices, using our fully segmented six-layered model, was consistent with MRI findings, showing increasing thickness moving up the processing hierarchy. In contrast, fronto-motor cortices showed the opposite pattern with changes in thickness of layers III, V and VI being the primary drivers of these gradients. As well as identifying key differences between sensory and motor gradients, our findings show how the use of this laminar atlas offers insights that will be key to linking single-neuron morphological changes, mesoscale cortical layers and macroscale cortical thickness.

Copyright

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