Multiple myeloma (MM) is a hematological malignancy of plasma cells that produce a monoclonal immunoglobulin protein. Despite significant advances in the treatment of MM, challenges such as resistance to therapy remain. Currently, inhibition of histone deacetylases (HDACs) is emerging as a potential method for treating cancers. Numerous HDAC inhibitors are being studied for use in monotherapy or in conjunction with other agents for MM. In this study, we investigated the anti-myeloma effect of Nexturastat A (NexA), a novel selective HDAC6 inhibitor...

BACKGROUND: Glioma is one of the most epidemic and obstinate types of cancer in the central nervous system with poor survival rate. Dacomitinib inhibited cell viability and proliferation of EGFR -amplified glioma. In the present study, the regional effects of Dacomitinib on tumor necrosis was investigated. METHODS: A C6 rat glioma model was evaluated using proton magnetic resonance spectroscopy (1H-MRS), diffusion weighted imaging (DWI), morphological T2-weighted imaging (T2W)...

The role of forkhead box P3 (FOXP3) protein in tumorigenesis has long been controversial and existing data on the association between FOXP3 gene polymorphisms and cancer susceptibility were inconsistent. Here, we conducted a meta-analysis to better clarify the relationship. A comprehensive search of studies published from July 2008 to June 2018 was conducted. The statistical analyses of the pooled odds ratios (ORs) and the corresponding 95% confidence intervals (95%CIs) were performed using the Revman 5.2 software...

Akirin1 is found to be involved in myoblast differentiation. However, the mechanism by which the Akirin1 gene regulates myoblast differentiation still remains unclear. In this study, we found that ectopic expression of Akirin1 promoted myoblast differentiation by increasing the expression of MRF4 and MEF2B mRNA. Additionally, we showed that ectopic Akirin1 induced cell cycle arrest by up-regulating p21 mRNA. To further uncover the mechanism by which Akirin1 promotes myoblast differentiation, we showed that the enhanced Akirin1 increased the mRNA expression of P38α...

The extensive use of fipronil (FPN) may trigger hazards to more than insects. The present investigation was carried out to evaluate the abrogating role of Terminalia laxiflora methanol extract against the neurotoxic effects provoked by FPN. Fourty male albino rats rats were assigned into four equal groups. The first group served as control, the second one was orally administered with FPN (10.5mg/kg BW), the third group was given combination of FPN and T.laxiflora methanol extract (TLE) (100 mg/kg BW) and the fourth one was orally given TLE...

BACKGROUND/AIMS: Studies have demonstrated that osteopontin (OPN) was associated with the severity and development of knee osteoarthritis (OA). METHODS: The purpose of this case-control study was to investigate the association between OPN gene rs11730582 polymorphism and knee OA risk in a Chinese population. Genotyping was analyzed using standard polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). RESULTS: This study found that C allele or CC genotype of OPN gene rs11730582 polymorphism was related to decreased risk for knee OA...

Previous studies investigate the relationship between peroxisome proliferator-activated receptor γ-2 (PPAR) gene Pro12Ala polymorphisms and risk of hypertension. However, the number of available studies was extremely limited. We updated this evidence and given more significant results. We performed comprehensive computer-based searches in the PubMed, Web of Science, Embase, Google Scholar, the Cochrane library, Wanfang database, China National Knowledge Infrastructure, and China Biological Medicine Database...

Using a case-control design, we assessed the association between single nucleotide polymorphisms of growth and differentiation factor 5 (GDF5) / rs143383 gene and interaction with environments and knee osteoarthritis. We recruited 288 knee osteoarthritis patients from the First clinical college, Henan University of Chinese Medicine between June 2017 and May 2018. There was significant difference in genotype distribution between case group and control group (χ2 =22.661, P =0.000). The minor C allele was significantly higher in the case group than that in the control group (20...

Unstable angina pectoris (UA) is one of the most dangerous clinical symptoms of acute coronary syndrome due to the risk of myocardial ischemia, which can lead to high morbidity and mortality worldwide. Though there are many advantages in understanding the pathophysiology of UA, the identification of biomarkers for the diagnosis, prognosis, and treatment of UA remains a challenge in the clinic. A global metabolomics research based on ultra performance liquid chromatography (UPLC) combined with time of flight mass spectrometry (Q-TOF/MS) was performed to discover the metabolic profile of health controls, UA patients, and UA patients with diabetes mellitus (DM), and screen for potential biomarkers...

BACKGROUND: Previous studies on the association between CYP1 polymorphisms and the susceptibility of prostate cancer showed inconsistent results. This study was conducted to derive a better estimation of the association. METHODS: A systematic search was carried out using the PubMed, EMBASE, Cochrane Library and CNKI databases for relevant articles published till August 15, 2018. Odds ratios, and 95% confidence intervals were obtained by fixed-effect or random-effect model...

BACKGROUND AND OBJECTIVE: The hepatitis C virus (HCV) is able to cause a life-threatening disease relating to lethal hepatocellular carcinoma. Previous, Toll-like receptor polymorphisms were proposed as promising biomarker for HCV-related hepatocellular carcinoma and disease progression. This study aimed to summarize the association of TLR4 polymorphisms and HCV infection through meta-analysis. METHODS: We applied a systematic review and meta-analysis performed by using PubMed, EMBASE, and Web of Science searches...

Osteoarthritis (OA) is a degenerative joint disease characterized by joint destruction with cartilage loss and occasional gross derangement of joint integrity. In recent years, several studies have reported the association between angiotensin converting enzyme (ACE) gene insertion/deletion (I/D) polymorphism and knee osteoarthritis (OA). However, the results were conflicting. To determine the association between ACE gene I/D polymorphism and knee OA, we conducted a hospital-based case-control study with 282 knee OA cases and 316 controls to investigate the association between ACE gene I/D polymorphism and knee OA susceptibility in a Chinese Han population...

GPR30, or G protein-coupled estrogen receptor (GPER), is a G protein-coupled receptor (GPCR) that is currently attracting considerable attention in breast cancer and cardiometabolic regulation. The receptor was reported to be a novel membrane estrogen receptor mediating rapid non-genomic responses. However, questions remain about both the cognate ligand and the subcellular localization of receptor activity. Here, we used HEK293 cells ectopically expressing N-terminally FLAG-tagged human GPR30 and three unique antibodies (Ab) specifically targeting the receptor N-terminal domain (N-domain) to investigate the role of N -glycosylation in receptor maturation and activity, the latter assayed by constitutive receptor-stimulated ERK1/2 activity...

Serine/threonine kinase 33 (STK33) is a serine/threonine kinase, and participates in many apoptotic process. Herein, we found that the extracellular signal-regulated kinase 2 (ERK2) was a substrate of STK33. STK33 phosphorylated ERK2and increased the activity of ERK2 and promote the tumorigenesis of colorectal cancer HCT15 cells. Clinical simple showed that STK33 was highly expression in colorectal cells and tissues. Ex vivo and in vivo studies demonstrated that STK33 accelerate tumorigenic properties in NCM460 cells and athymic nude rats...

BACKGROUND: There is a clinical need for the use of engineered adipose tissue in place of surgical reconstruction. We previously found that the external volume expansion (EVE) device increased special cell clusters in well-vascularized connective stroma during adipose regeneration. However, the origin of these cell clusters and their role in adipose tissue regeneration remains unknown. AIM: In this study, we evaluated EVE in the construction of expanded prefabricated adipose tissue (EPAT) in a rat model...

Connexins (Cxs) and pannexins (Panxs) are highly-regulated large-pore channel-forming proteins that participate in cellular communication via small molecular exchange with the extracellular microenvironment, or in the case of connexins, directly between cells. Given the putative functional overlap between single membrane-spanning connexin hemichannels and Panx channels, and cardiovascular system prevalence, we generated the first Cx40-/- Panx1-/- mouse with the anticipation that this genetic modification would lead to a severe cardiovascular phenotype...