Abstract

A protocol for rapid in vitro-multiplication of Plumbago zeylanica L. through axillary bud proliferation was developed as an essential prerequisite to conduct genetic transformation studies. A maximum of 3.5 ± 0.5 shoots were produced from a single nodal segment of a four year old field grown plant after 4 weeks of transfer to Murashige and Skoog's (MS) basal medium supplemented with 8.87 mmol/L BAP + 0.49 mmol/L IBA. Optimum root induction response was achieved upon transferring the individual regenerant to half strength MS medium containing 0.49 mmol/L IBA.