Objective

We'll be using the protocols we used on September 2nd. The only difference is that we'll use the stock concentrations that Professor Hartings made today (see below).

Description

Target Concentrations

Based on the total reaction volume that the class will be using and the final concentrations that will be in each reaction volume, I have determined that the following HAuCl4·H2O and Lysozyme masses are appropriate for today's stock solutions.

The actual masses are different from the target masses. The concentrations shown below reflect the actual concentrations made for the stock solutions. I used a 50 mL volumetric flask to make the gold stock solution and a 100 mL volumetric flask to make the protein solution. In each case I used HPLC-grade water.

Stock solutions

HAuCl4·H2O

50.38 mg

MW = 339.79 g/mol

Volume = 50 mL

2.96 mM

Lysozyme

28.69 mg

MW = 14307 g/mol

Volume = 100 mL

20.1 μM

Directions for making samples

Note: The final concentration of gold should be 0.25 mM and the final concentration of lysozyme should be 5.556 μM. This gives a Au:lysozyme ratio of 45, which should yield fibers.

1 mL samples

Volume of gold stock: 84.4 μL

Volume of protein stock: 276 μL

Volume of water: 640 μL

5 mL samples

Volume of gold stock: 422 μL

Volume of protein stock: 1,380 μL

Volume of water: 3,198 μL

Preparation of trypsin samples.

Weigh 15 eppendorf tubes and record their mass.

To each tube, add approximately 1mg of trypsin, weigh and record.

Calculate the concentration in each tube if 1mL of liquid were added.

Calculations to find concentration:

Concentration = [trypsin(g)/23'300g/mol]/0.001L

Matt Hartings What were the results of your syntheses? Where is the class UV-Vis analysis? What is the eppendorf mass data?

Data

The table below lists the protease samples we prepared and the mass of the corresponding eppendorf tube.

Mass of Eppy Tube (g)

Mass of Protease (g)

Concentraton (micromolar)

1.02691

0.00108

46.35

1.02622

0.00148

63.52

1.0224

0.00117

50.21

1.01944

0.00169

72.33

1.02922

0.00181

77.68

1.02625

0.00128

54.93

1.01909

0.00165

70.82

1.02036

0.00182

78.11

1.01922

0.00128

54.93

1.02024

0.00122

52.36

1.02097

0.00154

66.09

1.02422

0.00175

75.11

1.02669

0.00124

53.22

1.02936

0.00148

63.52

1.02923

0.00155

66.52

The table below lists the the class's eppendof tube masses (my groups data set are under 'RAM 9/15').

AMS 9/9

AMS 9/15

LMN 9/9

JBN 9/9

MB 9/9

RAM 9/15

AMS 9/16

1.02157

1.03107

1.03104

1.015

1.026

1.03005

1.03028

1.03162

1.03192

1.02859

1.01348

1.026

1.0255

1.02355

1.02798

1.02697

1.0199

1.0179

1.026

1.02676

1.02919

1.03275

1.01923

1.02753

1.02954

1.026

1.02869

1.02559

1.03057

1.02998

1.01646

1.0228

1.026

1.01656

1.01392

1.03059

1.03078

1.02048

1.026

1.01995

1.02938

1.02025

1.02419

1.01945

1.026

1.01707

1.02917

1.02932

1.01308

1.02723

1.026

1.02417

1.02993

1.03331

1.02896

1.02951

1.026

1.02518

1.02223

1.0287

1.0288

1.01517

1.026

1.01762

1.02055

1.02554

1.02602

1.02954

1.026

1.0349

1.03129

1.01372

1.02456

1.03211

1.026

1.03024

1.01359

1.01853

1.01719

1.0192

1.026

1.02016

1.02997

1.02051

1.02863

1.02781

1.026

1.0205

1.03117

1.03015

1.0285

1.026

1.02812

1.01972

Average Mass (g)

1.025135426

Standard Deviation

0.005373647

The table below is the raw data for the graph following it. The graph is a concentration vs absorbance of the class's compiled UV/Vis data at 280 nm.