Date and Time

Location

Description

Pipetting, Standard Curves and Spectrophotometry

How does a scientist measure concentration with light? In spectrophotometry, light passes through a sample and the amount absorbed tells us something about it. In this workshop you will learn to accurately use micropipettors and a spectrophotometer to analyze the concentration of an unkown. This is a workhorse laboratory technique and the basis of innumerable biological and chemical assays. You will get experience using micropipettes, the analytical balance, spectrophotometer and a graphing method of your choosing (paper, Excel or R).

About Lab Skills Workshops:

In this ongoing series of 1-2 day courses at Biotech without Borders, we’re inviting people of all skill levels together to complete a small teaching exercise in order to learn about laboratory work. We will cover the proper use of equipment and the real world application of the techniques being practiced.

Instructor Danny Chan received his MSc in microbiology during the course of his PhD candidacy at the University of Chicago studying the interaction of methicillin resistant staphylococcus aureus (MRSA) with a model system of organotypic human keratinocytes in an infectious disease focused department. He has been a research technician for many years in multiple fields including cellulostic ethanol production, protein crystallography, prefrontal cortex development and heat shock proteins. The common set of skills underlying his practice are molecular biology and scientific inquiry which he aspires to apply to society in order to foster new institutions of research and learning. Currently, he makes his living fact checking pharmaceutical ads in an agency while trying to defend his time to pursue independent research centered around protocol development for the DIY science community. You can follow his activities online at danwchan.ca or find him in person around NYC.