Begell HousePlasma MedicinePlasma Medicine1947-5764112011Cold Spark Discharge Plasma Treatment of Inflammatory Bowel Disease in an Animal Model of Ulcerative ColitisCold plasma discharges have been shown to have medically-relevant therapeutic effects when applied to living tissues, including blood coagulation and wound healing; thus, plasma treatment of inflamed tissues in ulcerative colitis disease may be an effective approach to reduce adverse consequences if not cure the disease. Here we discuss results of the first experimental study of cold plasma treatment of ulcerative colitis in a mouse model. As a plasma source, a cold spark discharge has been used. The results show that plasma treatment of experimental model of ulcerative colitis in mice has some beneficial effects by suppressing the progression of the disease while no damage to colon tissues is observed; and these effects are comparable to standard therapy.KalyanChakravarthySchool of Biomedical Engineering, Science and Health Systems, Drexel University, USADanilDobryninDepartment Electrical and Computer Engineering, College of Engineering, Drexel University, Philadelphia, PennsylvaniaGregoryFridmanNyheim Drexel Plasma Institute, Drexel University, Philadelphia, PAGaryFriedmanDepartment of Electrical and Computer Engineering, College of Engineering, A. J. Drexel Plasma Institute, Drexel University, Philadelphia, PennsylvaniaSreekantMurthyCollege of Medicine, Drexel University, USAAlexander A.FridmanNyheim Drexel Plasma Institute, Drexel University, Philadelphia, PA3-19Comparison of Point-to-Plane and Point-to-Point Corona Discharge for the Decontamination or Sterilization of Surfaces and LiquidsWe studied the decontamination of surfaces and inactivation of bacteria and yeast in liquids by low-temperature plasma generated in the DC corona discharge of an open-air type in the point-to-plane or point-to-point arrangement. We found that the inactivation in liquid suspensions is slightly more efficient with the point-to-plane discharge in comparison with the point-to-point one. In the case of agar surface decontamination, two types of inhibition zones were observed, which indicates the different mechanism of action for the point-to-point and point-to-plane discharges. This knowledge may be important in the future selection of microbicidal agents and in the development of efficient methods for low-temperature plasma decontamination or sterilization.VladimirScholtzDepartment of Physics and Measurements, Faculty of Chemical Engineering, Institute of Chemical Technology, Czech RepublicJ.JulakInstitute of Immunology and Microbiology, 1st Faculty of Medicine, Charles University, Czech RepublicB.StepankovaDepartment of Physics and Measurements, Faculty of Chemical Engineering, Institute of Chemical Technology, Czech Republic21-25Response of Human Glioma U87 Xenografted on Mice to Non Thermal Plasma TreatmentNon thermal atmospheric plasma is a new promising candidate in anticancer therapy. We have already reported the absence of skin damage induced by our plasma treatment. Preliminary results suggested that this treatment could also induce an antitumor effect on U87 malignant glioma xenografts, and we conducted this work to evaluate the antitumor efficacy of plasma in this model. Antitumor effects were assessed by tumor volume measurement and bioluminescence imaging (BLI). Plasma treatment was applied during five consecutive days in open air with a μs-duration pulsed floating electrode dielectric barrier discharge (FE-DBD), which delivered about 0.75 W at 200 Hz on the mouse skin. Our results showed a significant tumor volume decrease of 56% for treated mice at the end of the treatment, with a concomitant decrease of BLI intensity. Moreover, this tumor volume reduction translated into an increase of mouse life span of 60%, median survival being 9.5 and 15.0 days for control and plasma-treated mice, respectively. In conclusion, our study demonstrates a marked antitumor effect of plasma treatment in U87 glioma xenografts. These results, obtained in both a radio and chemoresistant model, are very promising and highlight the potential of plasma treatment as an anticancer treatment with little or no toxic side effects.MarcVandammeGREMI UMR-6606 CNRS. France, Université d’Orléans; and TAAM-CIPA, UPS44 CNRS, FranceEricRobertGREMI, Universite d'Orleans, Orleans, FranceSebastienDoziasGREMI UMR-6606 CNRS. France, Université d’Orléans, FranceJulienSobiloTAAM-CIPA, UPS44 CNRS, FranceStephanieLerondelTAAM-CIPA, UPS44 CNRS, FranceAlainLe PapeTAAM-CIPA, UPS44 CNRS, FranceJean-MichelPouvesleGREMI, UMR 6606, Universite d'Orleans, France27-43Targeted Cancer Treatment Using Anti-EGFR and -TFR Antibody-Conjugated Gold Nanoparticles Stimulated by Nonthermal Air PlasmaNonthermal air plasma killed G361 melanoma and SCC25 oral cancer cells targeted by antibody-conjugated gold nanoparticles. Although plasma alone is effective in killing cancerous cells, it also affects normal cells during the treatment process. For enhanced effects, gold nanoparticles and cancer-specific antibodies were pretreated before plasma treatment. Gold nanoparticles taken up by the cancerous cells are stimulated by the plasma treatment. Stimulation of gold nanoparticles results in an increase in death rate of cancerous cells. The selectivity of the killing process is achieved by conjugating gold nanoparticles with antiepidermal growth factor receptor and transferrin receptor antibodies. These conjugates can bind specifically to cancer cells. Gold nanoparticles stimulated by plasma kill these cancerous cells effectively. In this way, the killing efficiency of the plasma treatment process in the presence of conjugates is amplified about 18 times compared to the plasma treatment in the absence of conjugates. This technique shows the possibility of using plasma therapy for killing cancer cells selectively and effectively.GonJunKimDepartment of Electronic and Electrical Engineering, Pohang University of Science and Technology, SOUTH KOREAS. R.ParkDepartment of Oral Anatomy, School of Dentistry, Pusan National University, Republic of KoreaG. C.KimDepartment of Oral Anatomy, School of Dentistry, Pusan National University, Republic of KoreaJae Koo (J.K.)LeeDepartment of Electronic and Electrical Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea45-54Use of Proteomics to Investigate Plasma-Cell InteractionsThe understanding of basic mechanisms of plasma effects on living cells is one of the main preconditions to develop systematically innovative therapy options in the new and emerging field of plasma medicine. In this study, proteomics have been used for the first time to analyze the influences of physical plasma on vital constituents of mammalian cells. Treatment of human keratinocytes (HaCaT cells) by an atmospheric pressure argon plasma jet resulted in changes of charges of several cell proteins, but not in mass changes. These first results indicate plasma-induced reactions of functional groups or ligands, but no fragmentation, degradation, or complexation of cell proteins. Hereby, the importance to examine internal cellular changes caused by plasma treatment to elucidate the influence of plasma on the metabolism of human cells next to morphological changes, cell performance, and cell viability could be demonstrated. Starting from now, proteomics will become a useful tool for basic research in the field of plasma medicine.K.LandsbergLeibniz Institute for Plasma Science and Technology (INP Greifswald), and Institute of Pharmacy, Department Pharmaceutical Biology, Ernst Moritz Arndt University, GermanyCh.ScharfDepartment of Otorhinolaryngology and Head and Neck Surgery, Ernst Moritz Arndt University, GermanyK.DarmDepartment of Dermatology, Ernst Moritz Arndt University, GermanyK.WendeInstitute of Pharmacy, Department Pharmaceutical Biology, Ernst Moritz Arndt University, GermanyGeorgDaeschleinDepartment of Dermatology, University of Greifswald, Ferdinand Sauerbruchstrasse, 17475 Greifswald, GermanyE.KindelLeibniz Institute for Plasma Science and Technology (INP Greifswald), Greifswald, GermanyKlaus-DieterWeltmannLeibniz Institute for Plasma Science and Technology, 17489 Greifswald, Germany Thomasvon WoedtkeLeibniz Institute for Plasma Science and Technology e.V. (INP), Greifswald, Germany
55-63Analysis of Streamer Propagation for Electric Breakdown in Liquid/BioliquidThere is an increasing interest in the study of direct plasma generated in liquid/ bioliquid as it finds more applications in both industry and academic research. For all the applications, it is important to get a better understanding of the key physical mechanisms of the breakdown process. In the present paper, streamer propagation during an electric breakdown process of dielectric liquid was analyzed quantitatively using two different mechanisms based on electrostatic expansion and local heating. It was proposed that at the early stage of the streamer propagation, the electrostatic force due to the charging of a liquid-gas interface under a high electric field might be the major driving force for filament growth. Over a submicro-second time scale, the local heating might dominate the streamer propagation process, and the growth of the filament could be caused by the continuous evaporation of liquid at the tip of the streamer. Analysis of linear instabilities that lead to the bushlike growth of the streamers was carried out. Both classic Rayleigh-Taylor instability and electric field-induced instability were identified. It was shown that with an increasing applied voltage, the electrostatic instability was enhanced, whereas the Rayleigh instability was suppressed.YongYangDepartment of Mechanical Engineering and Mechanics, Drexel University, USAAndreyStarikovskiyDepartment of Mechanical Engineering and Mechanics, Drexel University, USAAlexander A.FridmanNyheim Drexel Plasma Institute, Drexel University, Philadelphia, PAYoung I.ChoDepartment of Mechanical Engineering and Mechanics, Drexel University, Philadelphia, USA65-83Plasma-Controlled Cell Migration: Localization of Cold Plasma−Cell Interaction RegionIn order to characterize the optimal condition for cell treatment with a plasma jet, its UV-vis-NIR spectrum was evaluated. Furthermore, this study considers the ability of cold atmospheric plasmas to impact cell migration rates as a function of (i) the length of the plasma treatment time, (ii) the number of hours after treatment that cell migration is assessed, and (iii) localization of the treatment zone. Data show that the ability of plasma to reduce cell migration rates increases as a function of treatment time with a maximum of 30%, and that this affect persists for 33 hours after plasma treatment.OlgaVolotskovaSchool of Engineering and Applied Sciences, Department of Mechanical and Aerospace Engineering, George Washington University, USAAlexeyShashurinThe George Washington University; School of Aeronautics and Astronautics, Purdue University, West Lafayette, IN, USAM. A.SteppSchool of Medicine and Health Sciences, George Washington University, USAS.Pal-GhoshSchool of Medicine and Health Sciences, George Washington University, USAMichaelKeidarDepartment of Mechanical and Aerospace Engineering, School of Engineering and Applied
Science, George Washington University, Washington, DC, USA85-92Live Pig Skin Tissue and Wound Toxicity of Cold Plasma TreatmentCold atmospheric pressure plasmas have emerged as a promising new tool for medical applications. Compared to conventional thermal plasma, such as arc coagulators and desiccators, cold plasma can be more selective in its application and may be used for effective sterilization of skin and wound tissue, wound healing and tissue regeneration, cancer treatment and blood coagulation. One of the key questions that has to be answered before these plasma technologies are introduced in medical practice is the safety of plasma treatment of living tissues, i.e. toxic dose levels of plasma exposure should be determined. It is well established that porcine (pig) skin closely resembles human skin; hence we evaluated the potential toxic effects of plasma treatment on intact and wounded skin in a Yorkshire pig model. Varying doses of Floating Electrode Dielectric Barrier Discharge (FE-DBD) and microsecond Pin-to-Hole Spark Discharge (PHD) plasmas were applied to determine a dosage regime where tissue damage occurs.DanilDobryninDepartment Electrical and Computer Engineering, College of Engineering, Drexel University, Philadelphia, PennsylvaniaAndrewWuDepartment of Surgery, Drexel University College of Medicine, Philadelphia, PA, USASameerKalghatgiDepartment of Biomedical Engineering, Boston University, Boston, Massachusetts; Department of Electrical Engineering, Drexel University; A. J. Drexel Plasma Institute, Drexel University, Philadelphia, Pennsylvania, USASinParkSchool of Biomedical Engineering, Drexel University, Philadelphia, PA, USANatalieChernetsThomas Jefferson UniversityKimberlyWaskoDepartment of Surgery, Drexel University College of Medicine, Philadelphia, PA, USAEsselDumaniDepartment of Pathology, Drexel University College of Medicine, Philadelphia, PA, USARobertOwnbeyDepartment of Pathology, Drexel University College of Medicine, Philadelphia, PA, USASuresh G.JoshiDepartment of Microbiology and
Immunology, Drexel University, Philadelphia, PA 19104RachelSensenigDepartment of Surgery, Drexel University College of Medicine, Philadelphia, PA , USAAri D.BrooksDepartment of Microbiology and
Immunology, Drexel University, Philadelphia, PA 1910493-108