Usage & Assay Information

The ED50 was determined by the dose-dependent stimulation of the proliferation of Mouse M-NFS-60 cells is 1 x 10^5 units/mg.

Storage & Safety

Storage

Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Endotoxin Levels

Endotoxin level is less than 0.1 ng per ug (1 EU/ug).

General Information

Biological Significance

M-CSF is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness, and male and female fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation.