A paper from the Monell Chemical Senses Center (Philadelphia) and the U.S. Department of Agriculture (USDA) reveals how infection with avian influenza (AIV) alters fecal odors in mallards.

“The fact that a distinctive fecal odor is emitted from infected ducks suggests that avian influenza infection in mallards may be ‘advertised’ to other members of the population,” notes Bruce Kimball, a research chemist with the USDA National Wildlife Research Center stationed at the Monell Center. “Whether this chemical communication benefits non-infected birds by warning them to stay away from sick ducks or if it benefits the pathogen by increasing the attractiveness of the infected individual to other birds, is unknown.”

“Avian influenzas are typically asymptomatic in ducks and waterfowl. Infection in these species can only be diagnosed by directly detecting the virus, requiring capture of birds and collection of swab samples," said Monell behavioral biologist Gary Beauchamp. "Our results suggest that rapid and simple detection of influenzas in waterfowl populations may be possible through exploiting this odor change phenomenon."

The official press release notes:

Using both behavioral and chemical methods, the findings reveal that AIV can be detected based on odor changes in infected birds.

In the study, laboratory mice were trained to discriminate between feces from AIV-infected and non-infected ducks, indicating a change in odor. Chemical analysis then identified the chemical compounds associated with the odor changes as acetoin and 1-octen-3-ol.

The same compounds also have been identified as potential biomarkers for diagnosing gastrointestinal diseases in humans. Kimball and colleagues hypothesize that metabolites resulting from viral infection interact in concert with bacteria in the gastrointestinal system of ducks to produce “odor signatures” indicating presence of the AI virus.

Future work will assess whether odor changes can be used for surveillance of AIV in waterfowl. In particular, researchers are interested in whether the odor change is specific to the AIV pathogen or if it is merely a general response to a variety of pathogens normally found in birds. Other studies will explore communicative functions of the AIV odor to gain greater understanding of how odors can shape social behavior in wildlife populations.