Abstract

The present investigation was carried out at “AGRI-BIOTECH FOUNDATION” in PJTSAU campus and College of Horticulture, Rajendranagar, Hyderabad during the year 2015 - 2016. The present study was conducted under four experiments for regeneration of capitulum explants in Murashige and Skoog medium supplemented with different hormonal concentrations with an objective to
determine the best concentration of growth regulators for culture establishment, shoots multiplication, rooting of in vitro shoots and
further to standardize suitable hardening media for establishment of gerbera plants. In the third experiment for standardization of
basal media for in vitro rooting, seven treatments were formulated with MS basal medium (control), MS medium + IBA, MS medium
+ NAA at different concentrations. Highest per cent rooting (83.33%), earlier rooting (19.00) with maximum root length (54.66 mm)
was obtained in MS medium supplemented with 2 mg/L IBA.