Validation of Nrt1p as the main DPI transporter. (a) Growth curves of wild type (ydl227cΔ/ydl227cΔ) and nrt1Δ/nrt1Δ yeast strains in the presence of various DPI concentrations. (b) Comparison of the maximum specific growth rate achieved by the WT strain in the presence
of various concentrations of DPI and the competitor nicotinic acid. (c) Comparison of the maximum specific growth rate achieved by the WT strain in the presence
of various concentrations of DPI and the competitor thiamine. Error bars = standard
error of the mean; n = 3.

F1 minimal medium. Components I, II and III can be made up together at 5× final concentration and autoclaved.
Component III can be made up at 5× final concentration and autoclaved. Component IV
(vitamin solution) is filter-sterilized and kept at -20°C; aliquots are added to fresh
1× solution. Component V is made up as 40% w/v stock solution and autoclaved.