Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

The PC-12 cell line was derived from a transplantable rat pheochromocytoma.

Clinical Data

male

Receptor Expression

nerve growth factor (NGF), expressed

Genes Expressed

catecholamines; dopamine; norepinephrine

Cellular Products

catecholamines; dopamine; norepinephrine

Tumorigenic

Yes

Effects

Yes, in New England Deaconess Hospital strain rats

Comments

The cells respond reversibly to NGF by induction of the neuronal phenotype when plated on Collagen IV coated culture flasks.

The cells do not synthesize epinephrine.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:

heat-inactivated horse serum to a final concentration of 10%

fetal bovine serum to a final concentration of 5%

Subculturing

Protocol: Volumes used for this protocol are for a 75cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.