Books-A-Million, the second largest brick-and-mortar bookseller in the U.S. with 251 stores in 30 states, has joined with Barnes & Noble in its fight against Amazon.com.

The Birmingham, Alabama company, which reported sales of $301 million for the first nine months of 2011, does not plan to carry titles from Amazon.com’s new publishing line or Houghton Mifflin Harcourt’s New Harvest imprint, according to a report by Publisher’s Weekly.

The report follows news last week in which Barnes & Noble announced that it won’t carry Amazon.com titles, with the company’s chief merchandising officer Jaime Carey saying that Amazon’s exclusive arrangements with publishers “have undermined the industry as a whole and have prevented millions of customers from having access to content.”

The decision by the top two booksellers in the U.S. could add more fire to the recent reports that Amazon.com is looking into opening its own retail stores, a topic we wrote about earlier today by suggesting the company may consider the University Village shopping center in Seattle.