Expression of cbfa1 gene in rat osteosarcoma UMR106 cell was detected by RTPCR after the 72? h's treatment of FSK88 liquid with different mol concentration(25,50,100?μmol/L),Retinoic Acid(RA,10?μmol/L) and mixed liquid of FSK88 and RA(1∶1).

Measurement of bone alkaline phosphatase and relative study with osteosarcoma

The objective of this paper is to explore the value of bone alkaline phosphatase (BALP) for diagnosing osteosarcoma, evaluating the effect of the chemotherapy, judging the prognosis and supervising the relapse and metastasis.

The immunoassay was used to check the BALP of the blood serum that was from 42 primary osteosarcoma patients.

BALP was more sensitive than ALP in diagnosing osteosarcoma (P = 0.015).

It has applied value in the diagnosis of osteosarcoma, reflection of the effect of chemotherapy and forecast the prognosis.

This is a preliminary report of X-ray diagnosis on osteopetrosis ga-llinarum. Twenty natural cases of the disease were discovered and studied by X-ray examination.Avian skeleton radiography is a useful and practicable aid to the diagnosis of osteopetrosis and is a more reliable method than others to detect lesions of the bones in live chickens.Radiographic signs of the disease are increase in density of bilateral bones, osteosclerosis with thickening of the cortex and stricture or obliteration of the medullary...

This is a preliminary report of X-ray diagnosis on osteopetrosis ga-llinarum. Twenty natural cases of the disease were discovered and studied by X-ray examination.Avian skeleton radiography is a useful and practicable aid to the diagnosis of osteopetrosis and is a more reliable method than others to detect lesions of the bones in live chickens.Radiographic signs of the disease are increase in density of bilateral bones, osteosclerosis with thickening of the cortex and stricture or obliteration of the medullary cavity. Pathologic changes of the skeleton were observed in most bones of the body, but they were most frequently encountered in the long bones of the extremities. The pathologic process affected the tibia first and its incidence was the highest (20/20), while the figures of other bones were, for the femur 12/20, the coracoid 11/20, the ulna 11/20 and the radius 11/20. The lowest incidences were encountered in the metatarsus 6/20 and phalanges 3/20. X-ray presentation of the disease might be divided into the following types. ( a ) fusiform deformity and sclerosis of the long bones in the extremities, especially in the metatarsus; (b)deformity of the long bones of the pelvic extremities with diffuse osteosclerosis; ( c ) difuse osteosclerosis without skeletal deformity; (d) partly proliferation and sclerosis in the endosteum or small focal osteosclerosis, which were ordinarily well-defined in the tibia and were sometimes found in the femur or ulna. The first type of X-ray presentation might only be in young chickens, while other types might generally occur in adult chickens without "bootlike" deformity of the metatarsus.Differential diagnosis of other avian osteopathies, for example, rickets, osteoporosis, periostosis, staphylococcosis, chronic osteomyelitis, osteosarcoma and Paget's disease was discussed. Radiopraphy of a shank is recommended as a means to detect this disease in general survey.

Since August 1969, radical local resection was done in 12 cases of malignant bone and soft tissue sarcomata in extremities. It is an en bloc resection including sarcoma per se and the infiltrated bone and soft tissue together with the surrounding normal tissue. The 12 cases included 2 cases of chondrosarcoma, 4 osteosarcoma, 1 each of giant-cell sarcoma and Ewing's tumor, and 3 soft tissue sarcomata (malignant fibrohistiocytoma, rhombdomyosarcoma and myxosarcoma). The reconstruction methods after local...

Since August 1969, radical local resection was done in 12 cases of malignant bone and soft tissue sarcomata in extremities. It is an en bloc resection including sarcoma per se and the infiltrated bone and soft tissue together with the surrounding normal tissue. The 12 cases included 2 cases of chondrosarcoma, 4 osteosarcoma, 1 each of giant-cell sarcoma and Ewing's tumor, and 3 soft tissue sarcomata (malignant fibrohistiocytoma, rhombdomyosarcoma and myxosarcoma). The reconstruction methods after local resection were: segmental resection and replantation of distal limb in 2, bone of the resected part boiled and reimplanted in 1, adjacent bone flap reversion in 1, massive homologous bone graft in 1, whole titanium artificial knee joint in 1, vascularized free fibular transplantation in 2 and transposition of m. sartorius and gracilis in 1. Reconstruction was unnecessary in the other 3 cases. All the 12 cases were followed for 16 months to 10 years. 2 chondrosarcoma cases, 1 giant-cell sarcoma, 3 soft tissue sarcoma and 1 fibrosarcoma of bone were living without local recurrence or lung metastasis, and they retained some function of the extremities. 3 osteosarocoma cases died of lung metastasis within 2 years and 1 Ewing's tumor showed bone to bone metastasis 1 year after operation, but they were free from local recurrence. Of the 12 cases, only 1 had local recurrence, probably due to insufficient resection.The authors hold that readical local resection is applicable to the treatment of malignant bone and soft tissue tumors of extremities instead of amputation. The latter often results in permanent disability and severe mental trauma, and also fatal lung metastasis is not prevented. Radical local resection is more suitable for tumors of low malignancy such as chondrosarcoma, malignant giant-cell tumor and certain soft tissue sarcomas. While for those of high malignancies such as osteosarcoma, amputation is preferred.

From May 1979 to Oct 1982, 48 infrahyoid myocutaneous flaps (IHMCF) were used for reconstruction after resection of lingual carcinoma in 44 cases, 4 for carcinoma of retromolar buccal mucosa, 3 each for carcinoma of floor of mouth and parotid gland and 1 each for osteosarcoma of mandible and soft tissue sarcoma of face. The operative technique was reported in 1980. Two flaps were used simultaneously in 4 cases of lingual carcinoma. When the width of flap was less than 4.5 cm, primary suture of the donor...

From May 1979 to Oct 1982, 48 infrahyoid myocutaneous flaps (IHMCF) were used for reconstruction after resection of lingual carcinoma in 44 cases, 4 for carcinoma of retromolar buccal mucosa, 3 each for carcinoma of floor of mouth and parotid gland and 1 each for osteosarcoma of mandible and soft tissue sarcoma of face. The operative technique was reported in 1980. Two flaps were used simultaneously in 4 cases of lingual carcinoma. When the width of flap was less than 4.5 cm, primary suture of the donor wound could be accomplished. The cutaneous cervical nerve was severed to increase the mobility of the flap. If there was no contraindication, the external and internal jugular vein or part of it including the superior thyroid vein were preserved for better venous return of the flap.