Nanopartz™ Plasmonic Fluorophores™

Nanopartz™ Plasmonic Fluorophores™ are fluorophore decorated gold nanorods manufactured using multiple patent pending and proprietary methods that result in fluorophores that exhibit much higher intensities than conventional fluorophores, no photobleaching even under the highest laser intensities, with chemical resistance to extreme pHs, salts, and other adverse chemicals. Peak fluorescence range from 550nm to 800nm, where each nanorod fluoresces up to 50,000 times brighter than a single fluorophore. Every batch is completely characterized including size, monodispersity, aggregation, and concentration. Residual chemicals are reduced to trace. A Certificate of Analysis (COA) is provided for every order exhibiting TEM and UV-VIS images and data, as well as DLS data. Conjugation options include amine, biotin, carboxyl, methyl, neutravidin, nhs, maleimide, streptavidin, and secondary antibody conjugations. These particles may also be conjugated to customer defined antibodies, peptides, oligos, and more. These conjugated particles can be used in darkfield, fluorescence, and other imaging modes; ELISA, lateral flow, and other assays.

These gold nanoparticles exhibit extinctions from 500nm to 2100nm, perfect for labeling in dark field, bright field, and fluorescence microscopy applications. These gold nanoparticles come with a wide variety of covalent conjugations, specifically suited for in-vitro applications. They are resistant to high salt concentrations, and a wide variety of pHs (4-9). The customer is given the opportunity to select from a variety of buffers, and may request custom conjugations to specific antibodies. Nanopartz possesses technologies that allow for the "site specific conjugation" to antibodies, thereby keeping the ligand active for further activity. Customer has a choice for radiation sterilization (no sodium azide is used) certification and endotoxin free products. >

It is less than the variablility of the size of the nanoparticles themselves, so it is unmeasurable.

How do I determine the concentration of the product?

Simply divide the number of nanoparticles (nps) found on the included COA by the amount of solvent you add.

Why do you sell your rods as OD-mLs and what does this mean?

This is because unlike spherical gold nanoparticles, the conversion from OD to mg is not linear with nanorod diameter. Consequently, for the same OD, the weight of the nanorods increases with diameter. 50 OD-mLs means that if you resuspend the material in 1mL of solvent, you will have an absorption of OD50 through a 1mL optical path.

Can I resuspend in water?

Yes

Can I suspend in silicon oil?

No

Will the SPR wavelength shift with different solvents?

The SPR is a function of the index of refraction of the host solvent in which the nanoparticles are suspended. Nanorods are the most sensitive and are therefore a great tool for measuring the index of refraction of the host.

"We have looked at many different gold nanoparticles samples from Nanopartz including spheres, rods, and microrods using single particle spectroscopy techniques and are extremely happy with the quality of the samples and the service provided by Nanopartz."

Stephan Link, PhD
Assistant Professor of Chemistry
Rice University

Plasmon-enhanced brightness and photostability from single fluorescent proteins coupled to gold nanorods

... Here, we use gold nanorods and single-molecule fluorescence detection to achieveplasmon-enhanced emission from intrinsically fluorescent proteins. ... Overall, our work indicates
that plasmonic particles improve both the brightness and photostability of FPs and extends the ...

Enhancing the optical excitation efficiency of a single self-assembled quantum dot with a plasmonic nanoantenna

... achieve this degree of control, emitters can be chemically conjugated to the plasmon resonant
structure ... The shallow depth of the emitters enables near-field coupling with plasmonic structures
placed on ... with a diameter of 90 nm as specified by the manufacturer (Nanopartz Inc.). ...

[HTML] Super-resolution of fluorescence-free plasmonic nanoparticles using enhanced dark-field illumination based on wavelength-modulation

... Even though many natural and synthetic fluorescent probes have been developed, most of ...
technique demonstrated the potential of super-resolution microscopy with fluorescence-free
material. ... and 80-nm SNP colloidal solutions were purchased from Nanopartz ™ (Salt Lake ...

Antibody-conjugated gold nanoparticles integrated in a fluorescence based biochip

J Ljungblad - 2009 - diva-portal.org

... field (Em) around the fluorophore in the ground state can lead to increased fluorescence. ...
Transmitted electrons are then focused on to a fluorescent screen to form an image. ... Citrate
stabilized and PEG conjugated gold nanoparticles were bought from Nanopartz, Salt ...

... during 3–6 h with the AuNP (8 μg/ml, diameter 100 nm, Nanopartz) in the ... For transfection, the
cells were observed by fluorescence microscopy 48 h after the treatment. ... by software (Image
Pro Plus, Media Cybernetics Inc.), taking into account the brightness of fluorescent cells of ...