One hundred and thirtyone faecal samples from cattle clinically suspected of having paratuberculosis were processed using the sedimentation culture method (SM) and the centrifugation culture method (CM) before inoculation into Herrold's egg yolk medium containing mycobactin J (M1), control medi...

The purpose of this study was to document the effect of calfhood vaccination for Mycobacterium paratuberculosis on a serologic ELISA. Fifteen calves vaccinated with a killed paratuberculosis vaccine and 5 unvaccinated control calves were tested from the first through the fifteenth month of life. Age...

A diet containing 11.5% (dry matter basis) of a moderately high Cd sewage sludge was fed to six Hereford steers for 106 days to simulate a high sludge intake from sludge-amended soils. At a mean live weight of 460 kg, the steers were slaughtered and liver, kidney, bone, muscle, lung, brain and splee...

OBJECTIVE: To describe the cross-sectional relationship between an objective measure of walking (pedometer-determined steps/day) and general indicators of health, a prior diagnosis of one or more components of the metabolic syndrome, and self-reported occupational activity in a generally sedentary w...

A dot ELISA was developed for detection of antibodies to Mycobacterium paratuberculosis. The assay was evaluated by testing sera from cattle that were determined, by bacteriologic culturing of feces, to be infected with M paratuberculosis and were suspected of having clinical disease. Further evalua...

To develop an animal model for sickle cell anemia, we have created transgenic mice that express a severe naturally occurring human sickling hemoglobin, Hb S Antilles. Due to its low solubility and oxygen affinity, Hb S Antilles has a greater propensity to cause red cell sickling than Hb S. To make t...

The purpose of this study was to compare the serologic responses of cows infected and non-infected with Mycobacterium paratuberculosis in infected and non-infected herds using different cut-offs between positive and negative samples. Infection status of cattle was determined by fecal culture. The se...

The purpose of this project was to identify the prevalence of feline immunodeficiency virus (FIV) in the Atlantic region of Canada, and to determine possible association between FIV serological status and breed, sex, and age. Feline serum samples (671) submitted to the Prince Edward Island Diagnosti...

All the cattle on 3 dairy herds endemically infected with paratuberculosis were tested 3 times at 6-month intervals by faecal culture and serological tests for paratuberculosis. Production data and somatic cell counts were also collected. Estimates of sensitivity relative to faecal culture were high...

The characterization of a purified antigen from Mycobacterium paratuberculosis, recently made commercially available for use in serodiagnosis by enzyme-linked immunosorbent assay (ELISA), of paratuberculosis in cattle was described. This assay had 89% specificity and 83% sensitivity for M paratuberc...

A telephone survey of three sub-populations in Ohio was conducted to compare the use of veterinary services in 1982 with 1983 and to investigate animal ownership. A response rate of approximately 90% was achieved for (1) food-animal producers, (2) horse owners and (3) pet owners. There was no signif...

The results of serological testing for paratuberculosis (AGID and ELISA) of 404 cattle were compared to faecal culture results. The AGID test was positive in 76 of 110 clinically suspect cows and in 6 of 30 subclinically infected cows from endemic herds. The AGID test was negative in all 102 cows fr...

In utero transmission of Mycobacterium paratuberculosis, the causative agent of paratuberculosis in cattle, has been suggested. Tissue specimens were obtained at a packing plant from pregnant dairy cows and their fetuses and from cows with clinical signs of paratuberculosis and from their fetuses. S...