MACSQuant® VYB Analyzer by Miltenyi Biotec

With unparalleled features, the MACSQuant VYB is set to excite the world of flow cytometry. The MACSQuant VYB is a compact benchtop flow cytometer featuring 3 lasers and detection of 10 optical parameters. With violet, yellow, and blue lasers the MACSQuant VYB is an ideal choice for multiparametric analysis of fluorescent protein expressing cells, stem cell phenotyping, cell cycle analysis, and apoptosis studies. Best in class benefits: • Discover new answers: Optimal for analysis of fluorescent... Read more

MACSQuant® VYB Analyzer

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Small bench top FACS that is robust and easy to use - highly recommended!

Rating: 5.0

Application Area: Flow Cytometry

"For the MACsQuant or the Vyb as we call it, is a solid work horse of the lab. It goes from 9am to 4pm non-stop most days. Our use is mainly characterization of hematopoietic cells and the occassional adherent cell line. For 3 colour analysis its perfect. We have the three laser version and there is no real need for compensation if you use the vioBlue, FITC (GFP) and then the Y4 (PE/APC-Vio770 conjugated antibodies) channels. Miltenyi has a great selection of antibodies as well. We also do four colours very simply when we have mCherry which is detected in the Y2 channel. It can also do high throughput and we routinely run 96 well plates. Note - if you do not have the optional refilling station the running buffer will run out about 3/4 of the way through so you need to be there to replace it. The FACS also has a mix option as well so there is no problem with the cells settling in the bottom of the well either.
Service: we have had no serious issues with the machine. Only once did we have serious blockage and at one stage one of the valves started to leak. But all were solved in a timely manner. They do come and update the software now and again but this also means you lose your data so you need to back this up.
Analysis: we do all this in FlowJo. You do need to do Miltenyi Transform to see the cells but otherwise that's the only tweak required. Do note that the resolution of populations is not as good as a high end BD machine. But I guess this is why this machine is essentially 1/4 the price?! One nice feature is that it gives you a cell concentration as well because it takes a certain volume for each sample rather than continuously aspirate until it gets the number of stated events. All in all my impression from using it the last few years is that it is a great machine for all our routine 3-4 colour work.
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"There are three lasers (405, 488, 561 nm), two scatter (FSC, SSC) and eight fluorescent channels, this allows for good flow cytometry results as compared to other 2 laser systems. It is fully automatic and there are cool color codes for each reagent which light up like mini disco lights.
It is fast, cell counts are available in less than a minute,"

"We bought the instrument for screening samples for expression of fusion proteins with fluorescent proteins. Its clever laser design allows detection of 4 different fluorescent proteins at the same time, reducing time analysis and increasing throughput. FCS files can be read in any flow cytometry analysis software. But currently we are using it for a lot of different applications, from cell cycle analysis from fish or plants to blood immunophenotyping."

"It is easy to use for automated sampling with a 96-well format. Automated staining of samples is possible. The after sale care is very good. However the software performance could be enhanced. An update to flowjo version 10 is a good step for the future improvements. It is very good value for money."

Best in class benefits: • Discover new answers: Optimal for analysis of fluorescent proteins • Trust the numbers: Absolute cell counts for any sample or population • Search deeper: Enhanced rare cell analysis with proven MACS® Technology • Achieve more: Automated analysis of up to 96 samples in a single run