Freedom Indiana campaign manager Megan Robertson speaks at an event Aug. 21, 2013, at the Indianapolis Artsgarden to launch the Freedom Indiana campaign that opposes a ban on same-sex marriage in Indiana.

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Opponents of Indiana's proposed amendment to ban same-sex marriage gained backing from another powerful business interest Tuesday when the Indy Chamber staked out its position for the first time.

The organization was the latest high-profile business interest to oppose the amendment, joining corporate heavyweights Eli Lilly and Co. and Cummins. The General Assembly will take up the measure for the second and final round of consideration early next year. If approved, the measure could then it go before voters in a statewide referendum in November 2014.