Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

ICC/IF

1/200.

ELISA

Use at an assay dependent dilution.

Dot Blot

Use at an assay dependent dilution.

WB

1/1000.

IP

1/200.

EMSA

Use at an assay dependent dilution. PubMed: 20606007

Target

RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.

The main difference between ab18184 and ab5000 is the immunogen used to produce the antibodies. ab18184 was produced against a 6X His synthetic peptide, while ab5000 was raised against a recombinant protein fused to a 6X His tag. Thus, ab18184 was orig...