Oxygenation set, to deliver CO2/O2 mixture: when a centralized distribution system is not available, the mixture can be delivered from cylinders kept in a safe
place near the lab bench and connected to the dispensing line (use a pressure regulator on the cylinder, e.g. AirCom model
RH200, http://www.aircom.net/, and adjust the pressure of gas mixture in the line)

Pressure transducer: a standard thin‐layer transducer can be used; the transducer should be sensitive enough to measure change
in pressure between 10 and 2 kPa (Statham model P23XL could be suitable; Grass Instruments)

5‐ml plastic syringes

Polygraph/chart recorder: use a pen‐writing polygraph (the two‐channel recorder model Gemini, from Ugo Basile, equipped with
interface with PC connection, is suitable to record a single experiment)