The Quantikine Human XCL1/Lymphotactin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Lymphotactin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Lymphotactin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Lymphotactin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Lymphotactin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates

Intra-Assay Precision

Inter-Assay Precision

Sample

1

2

3

1

2

3

n

20

20

20

20

20

20

Mean

0.92

2.83

5.57

1.01

3.08

6.29

Standard Deviation

0.024

0.078

0.116

0.095

0.278

0.494

CV%

2.6

2.8

2.1

9.4

9

7.9

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision

Inter-Assay Precision

Sample

1

2

3

1

2

3

n

20

20

20

20

20

20

Mean

1.12

3.27

6.66

1.14

3.32

6.69

Standard Deviation

0.031

0.085

0.184

0.084

0.199

0.374

CV%

2.8

2.6

2.8

7.4

6

5.6

Recovery

The recovery of human Lymphotactin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type

Average % Recovery

Range %

Cell Culture Media (n=4)

109

99-116

EDTA Plasma (n=4)

95

86-100

Heparin Plasma (n=4)

93

87-101

Serum (n=4)

98

89-107

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of human Lymphotactin were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: XCL1/Lymphotactin

Lymphotactin (Lptn), also known as SCM-1a and ATAC, belongs to the C or gamma subfamily of chemokines and has been designated XCL1. Human Lymphotactin encodes a 114 amino acid (aa) precursor with a 21 aa predicted signal peptide. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse.

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