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This study aimed at assessing the test-retest reliability of an exercise protocol designed to evaluate the effect of nutritional intervention on rehydration and endurance capacity after a 4 h recovery (REC) from prolonged running. Eight endurance trained male runners (age 29.3 íÓ2.7 years, height 170 íÓ2 cm, body mass 59.3 íÓ1.0 kg, VO2 max 63.9 íÓ2.3 ml·kg-1 · min-1; Mean íÓS. E. M.) performed a írecoverí protocol on two occasions, at least 7 days apart. The protocol consists of two parts. The first part (T1) required the participants to run at 70% VO2 max on a level treadmill for 90 min. Four hours later, participants performed the second part (T2) of the protocol in which they ran at the same speed to exhaustion, as a measure of their endurance capacity. During the REC, the participants ingested a fixed volume of fluid calculated from the fluid loss during T1. The physiological and metabolic responses were monitored throughout the run and the REC. The run time to exhaustion in T2 for Trial 1 was 55.1 íÓ6.3 min and 62.1 íÓ7.7 min for Trial 2 (íÓS. E. M.) (NS). The exhaustion run time of trial 1 was highly correlated to that of trial 2 (r = 0.929, p < 0.01). There were no differences between trials in heart rate, body mass change during exercise, rating of perceived exertion, or blood lactate and glucose concentrations during the test. It was concluded that the present protocol demonstrates satisfactory test-retest reliability for research on recovery from exercise in trained runners.