2019-05-25T18:05:46Zhttps://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1692402018-09-07T07:30:58Zcom_10261_112com_10261_1col_10261_365Jaraíz-Rodríguez, MyriamTabernero, María D.González-Tablas, MaríaOtero, ÁlvaroOrfao, AlbertoMedina, Jose M.Tabernero, Arantxa2018-08-30T07:32:32Z2018-08-30T07:32:32Z2017Stem Cell Reports 9(2): 451-463 (2017)http://hdl.handle.net/10261/169240http://dx.doi.org/10.13039/501100000780http://dx.doi.org/10.13039/501100003329http://dx.doi.org/10.13039/10000805428712848Connexin43 (CX43), a protein that forms gap junction channels and hemichannels in astrocytes, is downregulated in high-grade gliomas. Its relevance for glioma therapy has been thoroughly explored; however, its positive effects on proliferation are counterbalanced by its effects on migration and invasion. Here, we show that a cell-penetrating peptide based on CX43 (TAT-Cx43) inhibited c-Src and focal adhesion kinase (FAK) and upregulated phosphatase and tensin homolog in glioma stem cells (GSCs) derived from patients. Consequently, TAT-Cx43 reduced GSC motility, as analyzed by time-lapse microscopy, and strongly reduced their invasive ability. Interestingly, we investigated the effects of TAT-Cx43 on freshly removed surgical specimens as undissociated glioblastoma blocks, which revealed a dramatic reduction in the growth, migration, and survival of these cells. In conclusion, a region of CX43 (amino acids 266–283) exerts an important anti-tumor effect in patient-derived glioblastoma models that includes impairment of GSC migration and invasion.enghttp://creativecommons.org/licenses/by-nc-nd/4.0/openAccessA short region of connexin43 reduces human glioma stem cell migration, invasion, and survival through Src, PTEN, and FAKArtículo