Cholesterol imbalance is implicated in a variety of neurodegenerative diseases including Alzheimer’s Disease, Huntington’s Disease, and Multiple Sclerosis. Excess cholesterol is removed from the brain via the hydroxylation of C24 on cholesterol by CYP46 enzyme, a highly conserved member of the cytochrome P450 family, to the more soluble 24(S)-Hydroxycholesterol (24-OHC) which is able to cross the blood brain barrier.

Methods for testing transgene copy number and protein expression are necessary for development of genetically modified crops (GMO). Most transgenes impart herbicide resistance or protection from crop damaging insects and are proprietary.