Ifit1, 2, 3 and 3b are highly induced in cardiomyocytes during CVB3 infection.

Type I IFN-related gene expression was quantified by PCR array in the hearts of CVB3-infected (500 pfu i.p.) mice at 2 days p.i. (A) Schematic representation of the four different data sources subjected to PCR array analysis. (B and C) Only genes that are induced at statistically significant levels (P < 0.05) and more than 2-fold difference between Cre- and CMMCM mice are shown (n = 4–5, means ± SEM). (B) Heat map showing the fold-change of gene expression (compared to uninfected mice) after CVB3 infection in Cre- mice (left column) and CMMCM mice (right column). (C) Cardiomyocyte-specific changes in gene expression (left column of heatmap divided by right column of heatmap; IFIT genes are arrowed). (D and E) Primary cardiomyocytes were isolated from C57BL/6 (B6) mice and infected with CVB3 at MOI of 1. At indicated hours p.i., supernatants and cells were collected. (D) Infectious virus titers in the supernatant were determined by plaque assays and represented as PFU/ml. Data are representative of two independent experiments (n = 2, geometric means ± SD). (E) Real-time RT-PCR analysis of each IFIT family gene expression in primary cardiomyocytes. Each value was normalized to the values of Gapdh and divided by the values of uninfected controls (n = 1). (F) B6 mice were infected with 104 PFU of CVB3 i.p., and IFIT mRNA expression was analyzed 48 hours later by real-time RT-PCR. Each value was normalized to the value of Gapdh, and divided by the values of uninfected controls (n = 3, means + SEM).