Currently have a host with 3 data stores on 6.5 (VMFS5.60) and 5 VM's and want to migrate them to new Esxi Host running 6.7U2 (VMFS 6). Can I do a live migration? Have not installed VCenter on the new host yet. I have configured one large 6TB datastore.

If you have 3 6.5 hosts in a cluster with a 6.7U2 vCenter , and then you add your 6.7 Host to the cluster, and the 6.7 Host had all the same datastores, yes you could live migrate

If you have 3 6.5 hosts in a cluster with vCenter 6.5 and have 6.7 in another cluster with 6.7 vCenter and neither vCenter is connected... then no, you will not live migrate.

If you have 2 vCenters with an Enhanced Linked Mode configuration and you want to migrate from one cluster to the other, you may be able to do that using Cross Cluster vMotion. Even if they don't have shared storage you would have move Host and Storage at the same time. You would have to meet the requirements for this configuration.

Thanks Jason. These are standalone hosts. Current host has 6.5 and new host has 6.7U2. The environment is a police station so they run 24x7 hence my live migration question. I can take the systems down for a few hours if needed. Really need this to be simple and quick. If I can get the DC and File server moved and running first the other VM's can be done another time.

2) CPUs on both hosts must have similar feature sets (ideally the same, or at least the same vendor)

If you comply with 2 (CPUs match on both hosts), I suggest you deploy vCenter Server in eval mode, create a cluster and join both hosts in the cluster. If the old host has an older processor from the same vendor, add the old host first and set enhanced vMotion compatibility (EVC) on the cluster BEFORE adding the second host.

As the new host uses eval license, it gives you unlimited feature set. The old host needs at least Essentials Plus to do a shared-nothing vMotion. I can lend you a license key for a limited time to work around this. Send me a private message if you decide to go this way.