Application Information: TGIF antibody

WB (only tested on fusion protein): Use at a dilution of 1:2000. Colorimetric method for signal development.

Positive Controls

See Application Note

Form Supplied

Liquid

Concentration

Batch dependent
(Please refer to the vial label for the specific concentration)

Purification

Affinity purified with antigen

Purification Note

From polyclonal serum

Specifications: TGIF antibody

Full Name

TGFB-induced factor homeobox 1

Product Description

Chicken polyclonal to 5'-TG-3'-interacting factor

Background

Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Active transcriptional corepressor of SMAD2. Links the nodal signaling pathway to the bifurcation of the forebrain and the establishment of ventral midline structures. May participate in the transmission of nuclear signals during development and in the adult. as illustrated by the down-modulation of the RXR alpha activities.The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4. which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and eight variants. encoding four distinct isoforms. are described.

Synonyms

HPE4, MGC39747, MGC5066, TGIF

Host

Chicken

Clonality

Polyclonal

Target

5'-TG-3'-interacting factor

Isotype

IgY

Immunogen

Synthetic peptide - human 5'-TG-3' interacting factor

Antigen Species

Human

Species Reactivity

Human

Storage Conditions: TGIF antibody

Storage Buffer

Phosphate-Buffered Saline containing no preservatives

Storage Instruction

Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Notes

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.