Interleukin 1b is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. The gene encoding IL-1b and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Catalog #

I7663-11F

Applications

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution

ELISA: 0.2ug/ml

Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies

I1906 IgY, Chicken Pab Rb x Ch

I1906-10 IgY, Chicken-HRP Rb x Ch

I1906-12 IgY, Chicken-FITC Rb x Ch

I1906-14 IgY, Chicken-AP Rb x Ch

Storage and Stability

May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Clone Type

Polyclonal

Isotype

IgY

Host

Chicken

Source

Human

Concentration

~1mg/ml

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added. Glycerol free.

Purity

Purified by immunoaffinity chromatography.

Immunogen

Synthetic peptide corresponding to aa118-269 of human IL-1b

Specificity

Recognizes human IL-1b.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.