Endy:Standard for composable inverters

Contents

Steps

Set the PoPS_Low_min based on the detection level of GFP with a strong promoter. Should we use different reporters (lacZ and luciferase are more sensitive than GFP)?

Set the PoPS_High_min based on Reshma’s model (w/ elowitz GRF data providing the worst-case noise) given a desired reliability for X inverters in series and the PoPS_Low_min specified previously.

how many in series? Do we have enough play to have x => ∞?

Set the PoPS_High_max based on load limits. These load limits will be determined by the kinetic parameters of the repressor proteins (e.g. how many molecules you need to get repression), the strength of the RBS (tunable, though within some limits), and the total demand that the cell can experience without effecting growth rate.

What is the demand that the cell can tolerate before having a (>1%?) growth hit?

In polymerase or nucleotides sequestered / sec?

In ribosomes or AAs sequested / sec?

what are the boundaries on using a strong RBS to amplify a weak PoPS signal?