IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues. IDO-dependent suppression of T-cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T-cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.

Catalog #

044892

Applications

Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry and Western Blot. Other applications not tested.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Concentration

1mg/ml

Form

Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.

Purity

Purified by Protein A affinity chromatography.

Immunogen

Recombinant human IDO.

Specificity

Recognizes human IDO. Detects a band of ~45kD by Western blot.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.