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The other passengers were taken to area hospitals for injuries varying in severity.

Investigators were interviewing the bus driver, 56-year-old Jinli Zhao, who was not critically injured, authorities said.

The passengers were taking a three-day sightseeing tour to Washington that began on Friday in New York, authorities said. The crash happened as the bus was heading back to New York.

State police said there were no apparent witnesses outside the bus to the crash.

Elvis D'cruz, 19, said he was driving in the area with a friend when he came upon the overturned bus. He said he and his friend pulled over and were there before first responders arrived.

Seriously injured passengers were treated by the side of the road and lay down wearing neck braces as emergency crews arrived in the aftermath of the crash

A passenger is wheeled from the crash site on a stretcher as emergency responders attend the scene in Delaware

'Everyone was in pain and crying out for help,' said D'cruz, a student at Penn State Brandywine in Pennsylvania.

He said the group of passengers included mostly adults, many of them speaking different languages including Hindi, Mandarin, Spanish and Portuguese.

'There was not one person without blood on them,' he said, adding that he and his friend handed out items from a first aid kit.

D'cruz said the bus had overturned on an off-ramp from Delaware's Route 1 that is known for being steep.

Shavack said the bus belonged to Am USA Express Incorporated, a bus company based in New York.

Photographs taken at the scene showed the bus lying on the driver's side on a grassy shoulder. The photographs showed at least two people with neck braces lying in the grass while a group of others were sitting nearby.

Video footage taken at the site showed emergency officials leaning over to attend the injured and placing victims on stretchers as ambulances and other emergency vehicles stood by.

Debris was scattered about and a ladder had been set up alongside the overturned bus. Later photographs published online showed the bus had been righted.

The National Transportation Safety Board was expected to open an investigation, police told The News Journal.

The bus was taken away for further investigation on Sunday night following the crash which left two female tourists dead

The damaged bus was uprighted and being towed from the scene on Sunday following the horrific crash that left two women dead

Photographs taken at the scene show the bus lying on the driver's side on a grass bank while at least two people with neck braces are treated on the grass.

A spokesman for the New Castle County Department of Public Safety confirmed the death at the scene and said the rest of the passengers had been taken to nearby hospitals.

The official, Sgt. Michael McColley, says another 49 people were transported to area hospitals.

Delaware State Police spokesman Sgt. Paul Shavack says the accident did not involve other vehicles. He added that three to five others are in critical condition and that all of the passengers had been injured in 'some way'.

Two state police helicopters were used to transport people away from the site towards Christiana Hospital in nearby Georgetown.

The bus was said to be owned by AM USA Express, which is based in Chinatown, New York City. It was travelling southbound when it exited via a steep off-ramp and crashed.

State police told The News Journal of Wilmington that there were no apparent witnesses outside the bus to the crash.

But Elvis D'cruz, 19, told The Associated Press that he was driving in the area with a friend when he came upon the overturned bus. He said he and his friend pulled over and were there before first responders arrived.

'Everyone was in pain and crying out for help,' said D'cruz, a student at Penn State Brandywine in Pennsylvania.

He said that the group of passengers included largely adults, many of them speaking different languages including Hindi, Mandarin, Spanish and Portuguese.

'There was not one person without blood on them,' he said, adding that he and his friend handed out items from a first aid kit.

D'cruz said the bus had overturned on an off-ramp from Delaware's Route 1 that is known for being steep.