Hi there,
I want to get a 9mer oligonucleotide 5'-end hot labeled. I will use
this oligo as one strand of an adapter. I was wondering someones out
there have any experiences on that, particularly, 1) how to purify
labeled oligo from labeling reaction, and 2) that if I use unpurified
oligo to make my adapter, what is the effect on subsequent ligation?
Thanks in advance.
Peijun Zhang
pzhang at cc.umanitoba.ca