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Ameerega flavopicta is a dart-poison frog (Dendrobatidae) widely distributed throughout rocky habitats in the Brazilian states of Minas Gerais, Goiás, Tocanthins, Pará, and Maranhão. Here we repoert for the first record of a dendrobatid frog in the State of São Paulo, Brazil, based on individuals of A. flavopicta found in the Municipality of Pedregulho, and also describe the advertisement calls of a male from this population. A taxonomic discussion is provided.