The problem we're facing is that suddenly each network seems isolated (in the Microsoft Windows Network domain) and workstations/servers can only see their own network (for example 192.168.2.* can only see PCs on the 192.168.2.* and so on).

This doesn't affect the overall network since most of the services between servers and workstations work fine but certain software rely on the Windows Network to discover PCs (most importantly McAfee ePolicy orchestrator).

I'm not pointing the Windows Server 2008 AD as the source of the problem but it immediately occurred after switching to the new AD.

The information you've given seems to imply that it is NetBIOS browsing which is failing. If most services are working and being routed correctly between subnets, then it is not a network issue.

Standard NetBIOS announcement broadcast traffic does not cross between subnets, so the browse list from one subnet will not present itself to another.

To resolve this issue, you'll need to install the WINS Server role onto one of your servers. You then point (through DHCP for dynamic addressed clients) all the workstations and servers at the WINS server, and you should find your browse list comes back properly.

I expect the migration of servers failed to migrate WINS, which would explain this issue.

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None of the 5 FSMO roles do any form of tracking of network browsing themselves, although it is quite common for the holder of the PDC Emulator role to be nominated the 'Master Browser' for the domain automatically.

On the PDC Emulator role holder, check the Computer Browser service is set to 'Automatic' and is Started. This maintains the browse lists so must be running at all times.

If you wish to obtain browsing across subnets - or you have some sort of routing device between two segments of the network - a WINS server is required because the broadcast traffic cannot cross the subnet-boundaries and is not relayed by any routers.

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