The Groenkloof Nature Reserve, located adjacent to the Fountains Valley at the southern entrance to Pretoria, was the first game sanctuary in Africa. The reserve of 600 ha is managed by the Department of Nature Conservation. It is flanked by Christina de Wit Avenue and Nelson Mandela Drive, that separate it from the Voortrekker Monument and Klapperkop Nature Reserves. The reserve is open to day visitors from 5:30 to 19:00 in summer, and 7:00 to 18:00 in winter.

Since 1999 the reserve was stocked with various game species. These include zebra, blesbok, impala, kudu, blue wildebeest, red hartebeest (since 2002), giraffe (2002), sable (2003) and ostrich. Jackal, duiker and rock hyrax are also resident.