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Real-time PCR primer assays consist of unlabeled PCR primer pairs for use with dye-based chemistry such as SYBR® Green or EvaGreen®. Probe assays for real-time PCR and Droplet Digital™ PCR include PCR primers and a dual-labeled fluorescent probe with your choice of fluorophore. All PCR primers were designed to meet stringent performance standards following the MIQE guideline.
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We routinely use Southern Biotech's anti-human IgG-alkaline phosphatase as the secondary antibody in an ELISA designed for detection of dsDNA. The antibody is consistent and reliable in our protocol as outlined below.