Abstract

We recently developed a time-resolved multispectral laser-induced fluorescence (LIF) imaging system capable of tunable wavelengths in the visible region for sample excitation and nanosecond-scale characterizations of fluorescence responses (lifetime imaging). Time-dependent fluorescence decay characteristics and fluorescence lifetime imaging of apples artificially contaminated with a range of diluted cow feces were investigated at 670 and 685nm emission bands obtained by 418, 530, and 630nm excitations. The results demonstrated that a 670nm emission with a 418nm excitation provided the greatest difference in time-dependent fluorescence responses between the apples and feces-treated spots. The versatilities of the time-resolved LIF imaging system, including fluorescence lifetime imaging of a relatively large biological object in a multispectral excitation-emission wavelength domain, were demonstrated.

E. W. Chappelle, J. E. McMurtrey, and M. S. Kim, “Identification of the pigment responsible for the blue fluorescence band in laser induced fluorescence (LIF) spectra of green plants, and the potential use of this band in remotely estimating rates of photosynthesis,” Remote Sens. Environ. 36, 213-218 (1991).
[CrossRef]

1991 (2)

E. W. Chappelle, J. E. McMurtrey, and M. S. Kim, “Identification of the pigment responsible for the blue fluorescence band in laser induced fluorescence (LIF) spectra of green plants, and the potential use of this band in remotely estimating rates of photosynthesis,” Remote Sens. Environ. 36, 213-218 (1991).
[CrossRef]

Chappelle, E. W.

E. W. Chappelle, J. E. McMurtrey, and M. S. Kim, “Identification of the pigment responsible for the blue fluorescence band in laser induced fluorescence (LIF) spectra of green plants, and the potential use of this band in remotely estimating rates of photosynthesis,” Remote Sens. Environ. 36, 213-218 (1991).
[CrossRef]

E. W. Chappelle, J. E. McMurtrey, and M. S. Kim, “Identification of the pigment responsible for the blue fluorescence band in laser induced fluorescence (LIF) spectra of green plants, and the potential use of this band in remotely estimating rates of photosynthesis,” Remote Sens. Environ. 36, 213-218 (1991).
[CrossRef]

McMurtrey, J. E.

E. W. Chappelle, J. E. McMurtrey, and M. S. Kim, “Identification of the pigment responsible for the blue fluorescence band in laser induced fluorescence (LIF) spectra of green plants, and the potential use of this band in remotely estimating rates of photosynthesis,” Remote Sens. Environ. 36, 213-218 (1991).
[CrossRef]

Radiat. Environ. Biophys. (1)

Remote Sens. Environ. (1)

E. W. Chappelle, J. E. McMurtrey, and M. S. Kim, “Identification of the pigment responsible for the blue fluorescence band in laser induced fluorescence (LIF) spectra of green plants, and the potential use of this band in remotely estimating rates of photosynthesis,” Remote Sens. Environ. 36, 213-218 (1991).
[CrossRef]

Time-resolved F670 and F685 images spanning from 5 to 30ns in 5ns intervals for a representative apple artificially contaminated with the three feces dilutions, acquired using (a) 418, (b) 530, and (c) 630nm excitations.

(a) F670 and (b) F685 false-color fluorescence lifetime images of the representative apple artificially contaminated with the three feces dilutions. Each image contains the same apple excited by 418, 530, and 630nm from left to right.