Additional file 2.

Cell cycle progression in nocodazole-treated versus untreated cells. A PDF figure file showing cell cycle progression in nocodazole-treated versus untreated
cells. (a) Flow histograms plotting DNA content against cell number (left) and histograms
indicating the ploidy distribution (2C versus 4C; right) during a 12-h time course
of synchronized cells in the absence of nocodazole. At the later time points (t =
10 to 12), the level of synchrony decreased, indicated by the ploidy level equilibrium
reached at these time points, probably resulting from cells entering the next cell
cycle round, while other cells still have to pass through M phase. (b) Flow histograms
plotting DNA content against cell number (left) and histograms indicating the ploidy
distribution (2C versus 4C; right) during a 12-h time course of synchronized cells
in the presence of nocodazole. At the later time points, an increasing enrichment
of 4C cells can be observed because of a blockage of the cells at metaphase. Asterisk
marks the apparently lower proportion of 2C cells after a 20-h dark treatment in the
control series than in the nocodazole series, resulting from an acquisition artefact
during flow cytometry, indicated by the increased peak broadness in the respective
flow histogram.