Marian Burbine, 46, was indicted by a Middlesex grand jury on six counts of reckless endangerment of a child and two counts of operating an unlicensed day care business.

Prosecutors said Marian Burbine's unlicensed child care business allowed her husband, John Burbine, access to his young victims, who ranged in age from 8 days to 3 1/2 years old.

John Burbine pleaded not guilty to 40 counts of aggravated forcible rape of child, subsequent offense; 17 counts of aggravated indecent assault and battery on a child, subsequent offense; 12 counts of posing a child in a state of nudity; 11 counts of posing a child in sexual conduct; 13 counts of possession of child pornography; two counts of dissemination of material harmful to a minor and operating an unlicensed child care program. He was ordered held without bail.

"(John Burbine) gained access and opportunity to abuse young children through advertising child care services with his wife, who operated a tutoring and child care business, which was not licensed," Middlesex County District Attorney Gerry Leone said.

The assaults took place in the victims’ homes after the Burbines advertised child care and tutoring services on various websites, including parenting websites and coupon service websites, Leone said. The Burbines also transported children to another family’s home for day care and ran a summer activities program in which they took children to various places, Leone said.

The 100 charges are not the first child sex abuse charges brought against John Burbine. According to documents from Woburn District Court in 1989, Burbine entered an Alford Plea, in which he admitted that the prosecution had sufficient evidence to find him guilty of indecent assault and battery on a child younger than 14. He was given a six-month sentence and classified as a Level 1 sex offender.

Marian Burbine was freed on $1,000 bail following her Oct. 1 arraignment in Middlesex Superior Court. She was placed under house arrest and ordered to wear a GPS monitoring device as a condition of her bail. On Thursday, her house arrest terms were modified to allow her 90-minutes per week outside the home and to attend Sunday services.