Eurofins Genomics

Eurofins Genomics LLC is a global leader in DNA / RNA synthesis, sanger sequencing services, gene fragments, NGS, and many more high quality products and services. With production facilities in the United States, Europe, and Asia, Eurofins Genomics offers redundancy and risk mitigation for large companies and local relationships for small companies. The company’s strengths are its strong customer orientation, fast service, and high quality. Eurofins Genomics partners with major providers in the pharma, diagnostics, food, agriculture, biotechnological and academic research. Eurofins Genomics offers facilities with the following accreditations: ISO 9001, ISO 13485, CLIA, CAP, cGMP, GLP, and FDA accreditation.

About Eurofins Scientific

With over 20,000 staff in around 200 laboratories across 38 countries, Eurofins Scientific is the #1 provider worldwide for many different sectors... Show more »

About Eurofins Genomics

Eurofins Genomics LLC is a global leader in DNA / RNA synthesis, sanger sequencing services, gene fragments, NGS, and many more high quality products and services. With production facilities in the United States, Europe, and Asia, Eurofins Genomics offers redundancy and risk mitigation for large companies and local relationships for small companies. The company’s strengths are its strong customer orientation, fast service, and high quality. Eurofins Genomics partners with major providers in the pharma, diagnostics, food, agriculture, biotechnological and academic research. Eurofins Genomics offers facilities with the following accreditations: ISO 9001, ISO 13485, CLIA, CAP, cGMP, GLP, and FDA accreditation.

About Eurofins Scientific

With over 20,000 staff in around 200 laboratories across 38 countries, Eurofins Scientific is the #1 provider worldwide for many different sectors including food, environment, pharma, agroscience, genomics, pharmacology, pharmaceutical products testing, agroscience, central laboratory and more. In addition, Eurofins is one of the key emerging players in clinical diagnostic testing.

GeneStrands are linear, sequence-verified, double-stranded DNA fragments constructed using synthetic DNA and are ready for use in applications such as cloning, CRISPR-based genome editing, and antibody engineering.

GeneStrands serve as inexpensive substitute for gene synthesis. The specifications for GeneStrands gene fragments... Show more »

GeneStrands are linear, sequence-verified, double-stranded DNA fragments constructed using synthetic DNA and are ready for use in applications such as cloning, CRISPR-based genome editing, and antibody engineering.

GeneStrands serve as inexpensive substitute for gene synthesis. The specifications for GeneStrands gene fragments available from us are:

Sequence length between 100 bp and 2,000 bp.
Non-complex sequences only.
Delivered dry as a dsDNA fragment (min. 200 ng)
Order a GeneStrand with desired imbedded restriction sites and contruct your gene of interest in your own lab by cloning directly into your vector of choice using TA cloning, Gibson assembly, or other gene construction protocols.

Eurofins Genomics is the fastest provider for custom DNA oligos in the world. Furthermore, the company offers high-quality, longer oilgos named EXTREmers which are perfect for tough applications. As a pioneer in the art of high-throughput, automated custom oligo synthesis, Eurofins Genomics continues to develop innovative ways to... Show more »

Eurofins Genomics is the fastest provider for custom DNA oligos in the world. Furthermore, the company offers high-quality, longer oilgos named EXTREmers which are perfect for tough applications. As a pioneer in the art of high-throughput, automated custom oligo synthesis, Eurofins Genomics continues to develop innovative ways to serve its customers. Our scientists routinely use these oligos to perform DNA sequencing (using the Sanger method or Next Generation Sequencing technologies), cloning, gene synthesis, and qPCR services for our clients successfully.

Express Oligos are delivered the next day at no additional cost
EXTREmer oligos up to 200 mer
NGS Grade Oligos
Easy online ordering interface
Single stranded custom DNA in both tube and plate format
Synthesis scales from 0.01 μmol to 10 μmol
Huge variety of modifications
Huge variety of purifications
Delivered dry or wet (normalized)

Eurofins Genomics is the global leader in Sanger sequencing. The company offers long read lengths, fast, reliable turnaround time of results, and many exclusive prepaid options that researchers love. Our sequencing service has a success rate between 97.3% - 99% across all applications

Eurofins Genomics is the global leader in Sanger sequencing. The company offers long read lengths, fast, reliable turnaround time of results, and many exclusive prepaid options that researchers love. Our sequencing service has a success rate between 97.3% - 99% across all applications

Providing a fast and convenient way to sequence plasmids and PCR products, our automated DNA sequencing services are your best choice for fast and reliable DNA sequencing.

Barcodes for Unique ID
Eurofins Genomics provides bar-code labels for use with templates and primers that are submitted for DNA sequencing services. By using bar-coded labels, you guarantee that each sample and primer submitted to Eurofins Genomics for DNA sequencing has a unique identifier that is tracked throughout our automated system. Unique benefits include:

Overnight results (after receiving sample)
Free barcodes provided for full tracking of all samples
Over 80 universal primers available
Custom primers can be synthesized in the same facility quickly
Multiple reactions per sample is allowed
Prepayment option with the EVOcard
SimpleSeq sequencing kits
Prepaid plates

Any gene up to 1000 bp is synthesized for 35¢/bp in 8 days or it's free! Need it even faster? Cut that delivery time in half by using Express Genes.

Standard Genes up to 3000 bp

Eurofins Genomics offers standard genes which are available in any size up to... Show more »

Standard Genes up to 1000 bp in 8 Days or it's free!

Any gene up to 1000 bp is synthesized for 35¢/bp in 8 days or it's free! Need it even faster? Cut that delivery time in half by using Express Genes.

Standard Genes up to 3000 bp

Eurofins Genomics offers standard genes which are available in any size up to 3000 bp, with moderate GC content (40-65%), without extensive repeats (> 20 bp) or critical hairpin structures and without extensive homopolymer stretches (> 20 bp). Our proprietary software GeneOptimizer provides unique features for gene optimization. Furthermore, our experts take their time to craft longer genes which means you receive results you can trust.

Processing Standard Genes

Sequence Optimization (optional)

Our GeneOptimizer software will optimize the supplied amino acid or DNA sequences using our proprietary 4D™ Optimization process. Codons are optimized for the host species, repeats or hairpin structures are avoided, Gs and Cs are equally distributed, and specified motifs are avoided. GeneOptimizer is also available as free online tool for our customers.

Experimental Design

Overlapping oligonucleotides used to create the gene are designed by GeneOptimizer.

Oligonucleotide Synthesis

All oligoneucleotides are synthesized at our in-house DNA synthesis facility.

We ensure strict confidentiality of every project. The high quality standard of our service is permanently assured by professional quality management. The quality management system of our Huntsville, Alabama facility is certified to ISO 9001:2008 and ISO 13485:2003 quality standards.

You want to profile the expression of microRNAs (miRNAs) in your samples - then come to Eurofins Genomics!

Small non-coding RNAs are very short in length (17-25 nt). The sub-class microRNA (miRNA) is highly conserved and expressed in a tissue specific manner. It plays an important role in transcriptional and... Show more »

You want to profile the expression of microRNAs (miRNAs) in your samples - then come to Eurofins Genomics!

Small non-coding RNAs are very short in length (17-25 nt). The sub-class microRNA (miRNA) is highly conserved and expressed in a tissue specific manner. It plays an important role in transcriptional and post-transcriptional gene regulation and has been described to play important roles in cancer progression as well several hereditary diseases like progressive hearing loss.

Strict confidentiality is ensured for every project. The high quality of our service is overseen by a professional quality management team. We are certified according to DIN EN ISO 9001:2008 and ISO 17025:2005 (D-PL-13372-01-00).

Targeted gene expression analysis with qPCR aims at the quantitative measurement of mRNA expression of defined target genes (e.g. in different genotypes, prior to and during drug therapy or after the administration of compound candidates in the pre-clinical phase of drug development).

Targeted gene expression analysis with qPCR aims at the quantitative measurement of mRNA expression of defined target genes (e.g. in different genotypes, prior to and during drug therapy or after the administration of compound candidates in the pre-clinical phase of drug development).

Eurofins Genomics covers all steps in quantitative PCR (qPCR) - from the development of the experimental strategy to the final data analysis.

Based on the project, we use validated probes, customised probe designs or SYBR green assays for your quantitative PCR.

Strict confidentiality is ensured for every project. The high quality of our service is overseen by a professional quality management team. We are certified according to DIN EN ISO 9001:2008 and ISO 17025:2005 (D-PL-13372-01-00).

Copy Number Variations (CNVs) are structural alterations in a genome that results in an abnormal number of copies of one or more sections of the DNA. Copy Number Variations correspond to relatively large regions of the genome that have been deleted or duplicated.

Analysis of copy number variations via

Long-range PCR

Quantitative PCR

Microarrays

Next generation sequencing

Copy Number Variations (CNVs) are structural alterations in a genome that results in an abnormal number of copies of one or more sections of the DNA. Copy Number Variations correspond to relatively large regions of the genome that have been deleted or duplicated.

For genome wide association studies we offer the whole range of Affymetrix GeneChips and Illumina BeadChips

For genome wide association studies (GWAS) thousands or up to millions of genetic markers are genotyped in groups of individuals that either carry a certain trait (cases) or not (controls). Differences in the allele and... Show more »

For genome wide association studies we offer the whole range of Affymetrix GeneChips and Illumina BeadChips

For genome wide association studies (GWAS) thousands or up to millions of genetic markers are genotyped in groups of individuals that either carry a certain trait (cases) or not (controls). Differences in the allele and genotype frequencies between the different groups lead to the detection of genomic regions that are assiociated with the certain trait. GWAS typically focus on associations between single-nucleotide polymorphisms (SNPs) and traits like major diseases in humans or breeding traits in animals and plants.

Our GWAS support services comprise:

Affymetrix SNP mapping arrays

We offer the entire range of Affymetrix GeneChip and GeneTitan DNA analysis solutions, covering up to millions of markers in humans and many other species.

Illumina SNP arrays

We have extensive experience with Illumina’s BeadChips Infinium assays, which allow to genotype 50,000 to 5 million SNP markers per array.

Depending on your experience and current specifications we can either perform the analysis step or the whole Fragment Length Analysis (FLA) project for you.

3 different service packages for fragment length analysis (FLA) are available:

FLA - ready-to-load (RTL): We perform the capillary electrophoresis for you and deliver... Show more »

Depending on your experience and current specifications we can either perform the analysis step or the whole Fragment Length Analysis (FLA) project for you.

3 different service packages for fragment length analysis (FLA) are available:

FLA - ready-to-load (RTL): We perform the capillary electrophoresis for you and deliver first class results for your downstream analysis.

FLA service: You provide us with the PCR products and we take care of the subsequent fragment length analysis steps.

Full service genotyping project: Get in contact with our experts and design your personal FLA project.

Advantages of outsourcing your FLA project

Highest accuracy

All instruments used for fragment length analysis fulfill the highest demands of a forensic DNA lab. Our laboratory equipment is thoroughly monitored and the working routines are carefully documented by our project managers

Expertise and success even with challenging samples

Outstanding scientists and a superior technical staff perform all DNA profiling and microsatellite analyses. Our expertise is known and relied upon by the justice system, police departments and many food and biotech companies.

Trusted reliability

10 years of experience and 180,000 DNA samples per year exemplify our strength in genotyping DNA markers and profiling STRs.

Flexible capacities and fast results

An extensive range of equipment and sophisticated software solutions ensures the fastest turnaround times for every genotyping request.

Quality Management

Our quality management is ISO 9001:2008 certified. The laboratory is accredited according to ISO 13485:2003 for forensic genetics and food authenticity.

SNPs are our passion. With our services you can either take a deep look into the genome and search for unknown genotype-phenotype connections or genotype already known variations. No matter what, you always benefit from our fast turnaround times and high quality results.

SNPs (single nucleotide polymorphisms) or point mutations... Show more »

SNPs are our passion. With our services you can either take a deep look into the genome and search for unknown genotype-phenotype connections or genotype already known variations. No matter what, you always benefit from our fast turnaround times and high quality results.

SNPs (single nucleotide polymorphisms) or point mutations are the most common types of genetic variation determining to a major part the phenotype diversity between individuals. Causal point mutations change the amino acid sequence of the encoded protein and hence such SNPs are involved in the characteristics of an individual.

Depending on the analysed species these characteristics can be the resistance or susceptible to a certain disease, the body size or the eye colour in humans, the milk or meat yield in farm animals or the drought resistance and corn yield in crops.

We offer different genotyping technologies depending on the application and species, the purpose of the analysis, the number of SNPs per sample and the number of samples in the study. The choices included the following:

For genome-wide SNP genotyping, e.g. population studies, association studies, genomic selection including the analysis of 3k-3000k we recommend and offer Illumina BeadChips, Affymetrix GeneChips or Next Generation Sequencing.
For gene-wide SNP genotyping, e.g. fine mapping or haplotyping of candidate regions including the analysis of <500 SNPs we recommed Sequenom MassARRAY, Fluidigm Biomark, NGS or array-based technologies.
For the analysis of individual SNPs, e.g. determination of specific mutations in phamacogenetics or diagnostics with a small number of SNPs included, we offer Realtime PCR assays, Sanger Sequencing and the above mentioned technologies.

Order your next generation project with our bioinformatic services and get processed data that is easy to evaluate and interpret. Our outstanding expertise, state-of-the-art software, and certified hardware ensure that all your individual project data demands are... Show more »

Delivering comprehensive and high quality data analyses

Order your next generation project with our bioinformatic services and get processed data that is easy to evaluate and interpret. Our outstanding expertise, state-of-the-art software, and certified hardware ensure that all your individual project data demands are fulfilled.

Our bioinformatic services at a glance:

De novo assembly

Assembly of genomes of any size

Assembly of large insert constructs e.g. BACs

De novo transcriptome assembly to obtain full size transcript.

Mapping & variance analysis

Mapping of any re-sequencing data to a reference sequence

Detection of variances (SNPs, small InDels, and structural variances

Transcriptome analysis

Mapping of cDNA reads to a unigene set or an annotated reference genome

Analysis of expression profiles or splice variant

Amplicon variance analysis

Sorting and clipping of amplicon reads

Clustering of reads (without reference sequence)

16 / 18S or 22 / 28S phylogenetic analysis

Analysis of variances in comparison to a given reference sequence

BLAST analysis

Blast alignments against latest database releases

If you have special requirements for your bioinformatic solution, just contact us!

Besides their complete familiarity with the standard bioinformatics tools, our bioinformatics experts have developed software tools for a variety of typical and specialized applications including primer and microarray design, and next generation sequence assembly and analyses. The latter projects require utilization of industrial-scale bioinformatics computing power and algorithms. in addition to standard BLAST analysis, annotation, clustering and assembly services, our team can address your specific needs with a customized bioinformatics solution.

Annotation of prokaryotic genomes.

Strain comparisons EST2Genes.

Sequence analyses of metagenomes.

Clustering and assembly.

Customized bioinformatics services available.

Custom Projects

Analysis of data from a sequencing project requires the use of industrial-scale bioinformatics. Besides standard BLAST analysis, annotation, or clustering and assembly services Eurofins Genomics can help to solve your specific requests with a customized bioinformatics solution.

Eurofins Genomics has the expertise, the software, and the hardware to fulfill any of your bioinformatics needs.

Proven experience in prokaryotic and eukaryotic high quality de novo genome sequencing.

In close cooperation with our customers, we perform the following de novo genome sequencing projects:

Ultra high throughput sequencing of a shotgun library and assembly into contigs

Scaffolding of contigs by long paired-end sequencing

Closing of gaps based on Sanger technology

Bioinformatic analysis, e.g. annotation or comparison of different organisms

Resequencing Projects with the Technology of Choice

Depending on the aim of your studies, resequencing projects can be addressed using either Illumina HiSeq 2000 or Roche GS FLX technology:

Affordable resequencing with Illumina HiSeq 2000

For SNP detection and comparative analysis to a reference genome, we recommend the cost-efficient resequencing setup with Illumina HiSeq 2000 technology.

Sophisticated projects with Roche GS FLX

If large indels or genomic correlations between the sequenced and the reference genome are likely to be detected, sequencing on Roche GS FLX is the best choice. The longer reads of Roche GS FLX facilitate de novo assembly and subsequent comparative analysis. With this approach, you benefit from high quality genome sequences, even if large indels or genomic rearrangements occur.

Smaller-Sized Projects with Fast Turnaround Times with Roche GS Junior

The Roche GS Junior is the optimum system for the sequencing of smaller scale projects (e.g. BACs) that require only one-eighth of the capacity of a GS FLX Titanium run or would not be suitable for an Illumina run. As a consequence, the GS Junior adds a higher degree of flexibility in project planning and can have a positive impact on the turnaround time for small-sized projects.

Roche GS FLX+ sequencing is the technology of choice for de novo sequencing of genomes of any size. The long read length, together with our well-established expertise in long paired-end (LPE) libraries from 3 kb to 40 kb, guarantees the best data quality to provide high-quality assemblies. With the addition of the GS Junior to our... Show more »

Roche GS FLX+ sequencing is the technology of choice for de novo sequencing of genomes of any size. The long read length, together with our well-established expertise in long paired-end (LPE) libraries from 3 kb to 40 kb, guarantees the best data quality to provide high-quality assemblies. With the addition of the GS Junior to our sequencer fleet, we can offer small scale projects fast turnaround times.

Do you have a sequencing instrument but lack time or resources to establish certain library protocols? Let us enable your science advances with quality controlled and ready-to-sequence libraries. Save time by outsourcing those complex library preparation... Show more »

Empower your NGS sequencing with our expert library prep services

Do you have a sequencing instrument but lack time or resources to establish certain library protocols? Let us enable your science advances with quality controlled and ready-to-sequence libraries. Save time by outsourcing those complex library preparation procedures to Eurofins Genomics, your professional service provider.

Accelerate throughput on you own sequencing platform with our high-quality, ready-to-sequence libraries:

Confirm your plasmid constructs faster, more accurately and more affordably than ever before. Small and large constructs can now have the entire plasmid sequence done in a matter of days instead of weeks, without the hassle of having to design and synthesize primers. Eurofins uses a customized next-generation sequencing process to... Show more »

Confirm your plasmid constructs faster, more accurately and more affordably than ever before. Small and large constructs can now have the entire plasmid sequence done in a matter of days instead of weeks, without the hassle of having to design and synthesize primers. Eurofins uses a customized next-generation sequencing process to exceed the limitations, accuracy, and sensitivity of primer walking projects using sanger sequencing techniques. Our quality-focused process uses unique molecular barcodes assigned to each sample to assure proper sample identification. Our bio-informatics pipeline provides accurate data assembly compared to a reference sequence, variant calling, and allele frequencies.