Comparison of two immunoassays for quantification of hepatitis B surface antigen in Chinese...
Liu, Weiwei; Hu, Yao; Yang, Yannan; Hu, Tingting; Wang, Xinyu
2015-01-01 00:00:00
Two commercial quantitative immunoassay platforms are currently available for the detection of hepatitis B surface antigen (HBsAg) within a wide range of concentrations: the Architect HBsAg assay and the Elecsys HBsAg II. However, there are limited data concerning their performance in patients who are positive for both HBsAg and anti-HBs antibodies. In the present study, 127 serum samples from Chinese patients with hepatitis B carrying both HBsAg and anti-HBs antibodies were analyzed. HBsAg levels measured in parallel using the Elecsys HBsAg II and Architect HBsAg assay were compared. There was a significant correlation between HBsAg levels measured by Elecsys HBsAg II and Architect HBsAg assay (correlation coefficient, r = 0.992, P < 0.05). Bland-Altman analysis indicated only minor discordance between the methods (mean difference between Elecsys HBsAg II and Architect HBsAg assay, −0.02 log 10 IU/ml; 95 % confidence interval, −0.40–0.37 log 10 IU/ml). In conclusion, the results of the quantitative Elecsys HBsAg II were highly correlated with those of the Architect HBsAg assay in patients carrying both HBsAg and anti-HBs antibodies, but differences were observed between the platforms in samples with low HBsAg levels.
http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.pngArchives of VirologySpringer Journalshttp://www.deepdyve.com/lp/springer-journals/comparison-of-two-immunoassays-for-quantification-of-hepatitis-b-vQU3jaCD05