The importance of exosomes (small extracellular vesicles produced by most cell types) in the pathology of key diseases such as cancer have made them popular targets in both basic and pharmaceutical research. The Capturem Exosome Isolation Kit (Cell Culture) combines Capturem technology with a proprietary exosome-binding compound that is not antibody-based, allowing the isolation of exosomes from cell culture media in under 30 minutes.

The importance of exosomes (small extracellular vesicles produced by most cell types) in the pathology of key diseases such as cancer have made them popular targets in both basic and pharmaceutical research. The Capturem Exosome Isolation Kit (Cell Culture) combines Capturem technology with a proprietary exosome-binding compound that is not antibody-based, allowing the isolation of exosomes from cell culture media in under 30 minutes. The kit provides preclearing columns to remove large cellular debris and isolation columns to specifically isolate exosomes, both of which are used with a standard benchtop centrifuge, eliminating the need for ultracentrifugation. These maxiprep-sized columns allow the processing of 25 ml of cell culture supernatant at a time. Additionally, the kit contains wash and elution buffers that have been optimized for the isolation protocol.

The Capturem Exosome Isolation Kit (Cell Culture) provides a complete solution for the simple and rapid isolation of exosomes from cell culture media. The kit includes six single-use, disposable pre-clearing columns; six single-use, disposable isolation columns; as well as wash and elution buffers. The exosomes are isolated using specially designed Capturem spin columns containing a proprietary, exosome-binding compound which is not antibody-based. Each column will yield up to 2x1011 of purified exosomes in under 30 minutes.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Comparison of exosomes isolated using the Capturem exosome columns and two other methods

Quantitative determination of yield and mean size of exosome isolated through different methods. NTA data showing concentration (Panel A) and size (Panel B) of precleared CM (input) and isolated exosomes (yield) from HEK293T cells using the Capturem kit, precipitation reagent (Competitor T), and ultracentrifugation. The isolation methods are described in the Methods section of the technical note. The exosomes were isolated from 10 ml of precleared CM supernatant for each method. The total exosome yield was divided by input volume to obtain yield (particle/ml of input).

Western blot analysis for characteristic exosome proteins

Enrichment of exosomal markers CD81 and Alix in Capturem-isolated exosomes. Western blot analysis of CD81 and Alix as per the protocol described in the Methods section of the technical note. The band densities were calculated using Image Lab software.

Functional analysis of exosomes

Cellular uptake of exosomes in HEK293T cells. Isolated exosomes from the Capturem kit, ultracentrifugation, and input CM supernatant were labeled with PKH26 dye and uptake studies carried out as described in Methods section of the technical note. The percentage of total yield required for cellular uptake assay is displayed to provide a comparison of the yield differences between the two methods.

Overview

Simple, rapid protocol: isolate exosomes in under 30 minutes without incubations or ultracentrifugation

Complete solution: includes preclearing columns to remove large cellular debris, isolation columns to specifically bind exosomes, and optimized buffers for the isolation protocol

High yield: up to 2 x 1011 of purified exosomes per column, depending on cell type and cell culture conditions

Highly specific: exosomes are isolated using a nonantibody-based exosome-binding compound

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Applications

Additional product information

Please see the product′s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion.

Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).