Reconstruction and 3D-visualization of biological confocal data

In my work I meet with a number of issues relating to fluorescence and confocal imaging. Most of those experiments involve imaging of nerve cells (in vitro cultures) as well as entire sections of the brain (hippocampus). In our Laboratory experiments are also conducted on other types of animal and plant cells and tissues. During the conference, I would like to share with my experience on how to properly perform deconvolution process (appropriate setting of the microscope and its software) and the possibilities given by the modern software for 3D reconstruction and visualization in the context of three-dimensional presentation of the results. This poster will present photos of neurons from primary culture isolated from adult rat brain. Stain against NF-200 (Alexa 488), Octn3 (Alexa568) and nucleus (Dapi), taken with confocal SP5 microscope, processed in deconvolution software, reconstructed and enhancement in 3D software.