Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications have not been tested.

Recommended Dilutions

Flow Cytometry: Neat

Immunohistochemistry (Frozen): 1:3

Immunoprecipitation: 1:100

Western Blot: 1:100

Optimal working dilutions to be determined by researcher.

Hybridoma

NS1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Clone Type

Monoclonal

Isotype

IgG1

Clone No

3H511

Host

Mouse

Source

Human

Concentration

Not Determined

Form

Supplied as a liquid in PBS, 0.09% sodium azide. Also available without azide. See A0526-37C.

Purity

Ascites

Immunogen

Human Acetylcholine Receptor (AChR)

Specificity

Recognizes human AChR-gamma subunit found only in fetal AChR. Exhibits homogenous, high avidity binding to the receptor. Binds human recombinant gamma subunit on Western Blots. Does not react with mouse, rat or chicken.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.