Next-generation sequencing has transformed gene expression profiling and other RNA expression analysis studies. With an increasing number of researchers employing next-generation sequencing, and with the evolution of sequencing platforms, it is essential to use robust and streamlined methods for sample preparation. The best next-generation sequencing data begin with optimal samples.

RNA Sequencing Overview

Next-generation sequencing studies require robust and streamlined methods for sample preparation. The best next-generation sequencing data begin with optimal samples. Click the tabs above to learn more and see all available products.

RNA Isolation

Although many methods are available for purifying RNA from any particular source, obtaining RNA of the highest purity is the best assurance that the downstream manipulations will be successful. Choose from various formats, including PureLink®, mirVana™, RecoverAll™, and MagMAX™ kits to isolate total RNA, then enrich for the subpopulation of interest.

RNA Enrichment

To maximize the effectiveness of sequencing reagents, it is often necessary to enrich the sample for specific regions or targets of interest. This enrichment helps eliminate nonspecific artifacts, making it possible to better analyze smaller changes in genomes or transcriptomes and less abundant sequences. Ambion® RiboMinus™ technology is designed to enrich the whole spectrum of RNA transcripts, regardless of their polyadenylation status or the presence of a 5' cap, by selectively depleting rRNA. The RiboMinus™ method removes the vast majority of rRNA (up to 99.9%) to allow greater interrogation of the less abundant transcripts.

RNA Library Construction

Library construction, template preparation, and sequencing are at the heart of the sequencing workflow. Utilization of sequencing controls and methods for data analysis complete the workflow and provide useful annotated data. Generate cDNA by reverse transcription from adaptors ligated to the ends of RNA, then amplify the cDNA using primers complementary to the adaptors, and purify it.

RNA Library Controls

Library construction, template preparation, and sequencing are at the heart of the sequencing workflow. Utilization of sequencing controls and methods for data analysis complete the workflow and provide useful annotated data.

Learn more about ERCC Controls

2 tubes containing preformulated mixes of the same 92 RNA transcripts but in different ratios, for measuring dynamic range, assessing bias, and confirming expression fold changes

RNA Barcoding

Sets of 16 barcodes are available for use with the SOLiD® Total RNA-Seq Kit or the SOLiD® SAGE™ Kit with Barcoding Adaptor Module. Barcodes cost-effectively increase the number of samples that can be analyzed in a single sequencing run. Barcodes assign a unique identifier to templated beads made from a single library. Once assigned, the RNA libraries can be pooled and sequenced.

RNA Sequencing

No matter which platform you choose to suit your needs, we continue to support the evolving needs of this field with gold-standard instrumentation, innovative next-generation technology, optimized best-in-class reagents, consumables, analysis software, applications and world-class technical support.