RE: melanin bleach query

From:

Emma Carter <E.Carter@oxfordbiomedica.co.uk>

> One of our pathologists needs a melanin bleach performed on sections
> of
> lymph node requiring IHC (S-100 and HMB45). From previous knowledge, I
> recollect that if done prior to the IHC stain, the tissue washes off,
> however, by doing it post IHC, would the chromagen (DAB) still be
> intact?
> Will there be any fading of positive staining due to this procedure.
>
Have you considered using a nickel or cobalt step to turn the
DAB black instead of staying brown....
emma carter
Oxford BioMedica (UK) Ltd