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Materials and Methods

In this experimental study, peripheral neuropathic pain associated with sciatic nerve chronic
ligation (SN-CL) was induced. Filaments used against posterior pad mid-plantar region using a simplified up-down
method (SUDO). In addition to baseline withdrawal thresholds, the behavioral test was repeated after surgery thrice
more with an interval of ten days. vFF (2 to 26 g) were used in ascending order for hyperalgesia assessment.

Results

In SN-CL rats, the results validate a loss of pain sensation, resulted in, long-lasting ipsilateral allodynia with
the development of contralateral allodynia later and an extraterritorial development of neuropathic signs. Variability
for the development of ipsilateral and contralateral allodynia over time was noted in sham (SH) control rats. SN-CL
group showed a contralateral hyperalgesia development just at the 16th-day after surgery with an absence of ipsilateral
hyperalgesia development at the different days of paw withdrawal thresholds measurements.

Conclusion

Manually vFF test technique was successfully used for assessing neuropathic pain behavioral signs in
sciatic a nerve ligation model with the absence of ipsilateral hyperalgesia development.