Revision as of 17:16, 11 April 2013

Contents

Overview

Image Quantification Analysis Protocol
This protocol is to establish the light densities of images collected by fluorescence microscopy and their significance. This is most often done as part of the plate controls in a RNAi experiment (L4440 empty vector against GFP).

Enter data according to instructions into the graphpad t test calculator

Make graph

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

The goal in imaging is for all the images to be placed the same

If the worm's til is interfering in the box, use the color selector tool to select the background then use that color to pint over unwanted lit areas with the paint brush tool (double click to change stroke size). Click and hold to draw.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.