The depositor states that this cell line is negative for the presence of Epstein-Barr virus(EBV) genome.
ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR.
The SU-DHL-16 cell line possesses a t(14;18)(q32;q21) translocation.
The SU-DHL-16 cell line contains a minor bcl-2 rearrangement.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Cultures can be maintained by the addition of fresh medium. An inoculum of 1 X 105 to 2 x 105 cells/mL is recommended. Subculture when cell concentration is between 1 x 106 and 2 x 106 cells/mL.Subcultivation ratio: A subcultivation ratio of 1:4 to 1:10 is recommended.Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.