To evaluate the diagnostic potential of meat juice for early detection of Classical swine fever virus (CSFV), meat
juice and serum samples from pigs experimentally infected with different strains of CSFV were compared for virus load. From
all samples, viral RNA was extracted by automated procedure before real-time reverse transcription polymerase chain reaction
analysis was performed. Viral RNA was detected in meat juice, but at a lower level than in corresponding serum. Sensitivity
was calculated to 91% and specificity to 97%. Disagreements between meat juice and serum results were found when samples
originated from pigs infected with low virulence CSFV strains and/or when samples were collected within the first days after
infection. In conclusion, while not the first choice for sample material for CSFV diagnosis, meat juice may constitute a useful
alternative for herd-based studies or when blood and/or target organ material is not available. Strain virulence and time points
for sample collection after infection are factors of importance for diagnostic success.