Q-What if my fragments contain repetitive sequences?

A- Since most commercially available seamless assembly kits, including our GeneArt® Seamless kits, are based on homologous recombination, cloning and assembly of fragments with repetitive regions could result in rearrangements in your final construct. Life Technologies will have a cloning and assembly kit for repetitive sequences in 2013.

Q- Can I clone 5 fragments using the seamless assembly kit, even if it’s not supported by your web tool?