Representation oligonucleotide microarray analysis

Representational oligonucleotide microarray analysis (ROMA) is a technique that was developed by Michael Wigler and Rob Lucito at the Cold Spring Harbor Laboratory (CSHL) in 2003. Wigler and Lucito currently run laboratories at CSHL using ROMA to explore genomic copy number variation in cancer and other genetic diseases.

In this technique, two genomes are compared for their differences in copy number on a microarray. The ROMA technology emerged from a previous method called Representational Difference Analysis (RDA). ROMA, in comparison to other comparative genomic hybridization (CGH) techniques, has the advantage of reducing the complexity of a genome with a restriction enzyme which highly increases the efficiency of genomic fragment hybridization to a microarray.

In ROMA, a genome is digested with a restriction enzyme, ligated with adapters specific to the restriction fragment sticky ends and amplified by PCR. After the PCR step, representations of the entire genome (restriction fragments) are amplified to pronounce relative increases, decreases or preserve equal copy number in the two genomes. The representations of the two different genomes are labeled with different fluorophores and co-hybridized to a microarray with probes specific to locations across the entire human genome. After analysis of the ROMA microarray image is completed, a copy number profile of the entire human genome is generated. This allows researchers to detect with high accuracy amplifications (amplicons) and deletions that occur across the entire genome.

David Rossi

David Stephen Rossi is a fictional character in the CBS crime drama Criminal Minds, portrayed by Joe Mantegna. He is a Supervisory Special Agent of the FBI's Behavioral Analysis Unit, and has appeared from the episode "About Face", which was originally broadcast on October 31, 2007, during the show's third season.

Rossi begins the series returning to the FBI after a lengthy period of being semi-retired, with his return due to "unfinished business". He is shown to be emotionally tied to Aaron Hotchner, as they both worked together during the early days of the Behaviorial Analysis Unit. Rossi is also a writer, and is one of the team's senior and most decorated profilers. He replaced Jason Gideon, who was written out following Mandy Patinkin's abrupt departure from the series.

Mantegna has stated in an interview that the character was named after one of the policemen who had testified at the O. J. Simpson trial. Rossi's middle name, Stephen, was revealed in a flashback near the end of the tenth season episode, "Nelson's Sparrow." After Rossi has deduced that Jason Gideon is about to become a father, Gideon asks Rossi what his middle name is. This is followed by a present-time scene where Rossi greets Gideon's son, Stephen, by name. (The "ph" spelling of the name was used in the episode's end credits.)

RAI operates many DVB and Sat television channels and radio stations, broadcasting via digital terrestrial transmission (15 television and 7 radio channels nationwide) and from several satellite platforms. It is the biggest television broadcaster in Italy and competes with Mediaset, Sky Italia, and other minor television and radio networks. RAI has a relatively high television audience share of 33.8%.

A Day At A Time

Living a day at a time be the rhythm that fitThat way I´m always quick to predict a glitchBuilding a house brick by brick and bit by bitLiving a day at a time, a day at a timeLiving a day at a time be the rhythm that fitThat way I´m always quick to predict a glitchBuilding a house brick by brick and bit by bitI light a thick spliff get my wit in pitchTime goes by so fast I lose hold of myself / Needsomething to cool me down,or my motor will melt / Totally set on not getting oldyet / but time willcatch up with you like an old debt / Still... hurryspells worry with a W,you heard me? It´s too early, my world be looking allblurry / Listen, waita minute / I got a job that I aim to finish / ain´t gottime for no lazybusiness / I´m losing crazy minutes the way I sleep allday and shit /Professional time waster / sitting here wondering whythe earth keepworshipping that almighty paper / Quick, fast, world onthe move / Think ofyourself, no room for the merciful / Gotta be versatile/ Being everywhere,doing everything, at the same time / Don´t waste timeon that lazy state ofmind now / All cats ain´t got nine lives now / It´s nowor never so betterjust get it together / You ain´t gone be living forever/ I know this / Theclock stay ticking and I can´t control this / Can´t beliving at a low risk/ so I might as well roll this / Oh yes / reminisce onsome old shit / myflow is reaching your sofas / all of my mentalsoldiers.Living a day at a time be the rhythm that fitThat way I´m always quick to predict a glitchBuilding a house brick by brick and bit by bitLiving a day at a time, a day at a timeLiving a day at a time be the rhythm that fitThat way I´m always quick to predict a glitchBuilding a house brick by brick and bit by bitI light a thick spliff get my wit in pitchI be telling you listen I don´t lieTime always be keeping a close eyeYou don´t believe me then you take a nosediveSlow down, don´t you know nowChasing euros or dollars or then you chase poundsHurry, hurry you keep up the pace nowYou ain´t really with it I see it in your face nowSlow down, don´t you know nowNow all sorts of folks die / Think I´m lying then askthe Most High / Openyour mind / c´mon wake up open your eyes / Realize youtaking a nosedive / Iwon´t resign, won´t recline / Kinda the same as timewon´t rewind / But I´ma relax / ´cos if you thinking like that then yourstress will multiply / Isuppose I´m saying I ain´t scared to die / ´cos thenext place might be thebest place, still I don´t waste time estimating / Soget your bets placed in/ It´s the game of life, for life / all day, all night/ Remain your fightand you be set for escalating / Guys defecating bypaper chasing, I ain´tgot time for that / I need a real woman I ain´t gottime to mack / Spendinghalf of my time in a cold climate / Time´s back / I waslooking for time butnow I know where time´s at / Where´s that? / It´s righthere betweenyesterday and tomorrow / you follow? / Time will go bywhether you dating amodel / or you got problems and everyday you escapewith the bottle / Idon´t know why, though / I just glide slow through mylife / time forhappiness, time for sorrow / Living a day at a time...Living a day at a time be the rhythm that fitThat way I´m always quick to predict a glitchBuilding a house brick by brick and bit by bitLiving a day at a time, a day at a timeLiving a day at a time be the rhythm that fitThat way I´m always quick to predict a glitchBuilding a house brick by brick and bit by bitI light a thick spliff get my wit in pitchNow whether you spitting them dope rhymes.Or working the coalmineOr sniffing them coke linesOr sleeping the whole timeBreeding or killingWalking the narrow road or sinningLosing or winning

In August 2016, a research plane was able to observe something strange in the atmosphere above Alaska's Aleutian Islands, lingering aerosol particle that was enriched with the same kind of uranium used in nuclear fuel and bombs, according to Gizmodo. The observation was the first time that scientists detected a particle free-floating in the atmosphere in over 20 years of plane-based observations ... ... -WN.com, Maureen Foody....

ADDIS ABABA, Ethiopia (AP) -- Ethiopia's defense minister on Saturday ruled out a military takeover a day after the East African nation declared a new state of emergency amid the worst anti-government protests in a quarter-century. The United States said it "strongly disagrees" with the new declaration that effectively bans protests, with a U.S ... He also ruled out a transitional government ... Learn more about our and . ....

One day in August 1995 a man called Foutanga Babani Sissoko walked into the head office of the Dubai Islamic Bank and asked for a loan to buy a car. The manager agreed, and Sissoko invited him home for dinner. It was the prelude, writes the BBC's Brigitte Scheffer, to one of the most audacious confidence tricks of all time. Over dinner, Sissoko made a startling claim ... With these powers, he could take a sum of money and double it ... ....

MEXICOCITY. A strong earthquake shook southern and central Mexico Friday, causing panic less than six months after two devastating quakes that killed hundreds of people. No buildings collapsed, according to early reports ... We start crying whenever the (earthquake) alarm goes off,” 38-year-old publicist Kevin Valladolid told Agence France-Presse through tears after evacuating from his building in La Roma, in central Mexico City ... AFP ... ....

Mexico City – A military helicopter carrying officials assessing damage from a powerful earthquake crashed Friday in southern Mexico, killing 13 people and injuring 15, all of them on the ground. The Oaxaca state prosecutor’s office said in a statement that five women, four men and three children were killed at the crash site and another person died later at the hospital ...Alejandro Murat, neither of whom had serious injuries ... The U.S ... ....

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ROME (AP) — The rapid development of 20-year-old Turkey winger Cengiz Under is playing a big role in Roma's revival. Under scored his fourth goal in three matches and Roma won at Udinese 2-0 for its third straight win in Serie A on Saturday. Roma moved into third place, two points ahead of Inter Milan, which lost at Genoa 2-0 ...Roma went ahead when Under unleashed a powerful, rising shot from beyond the area in the 70th....

Turkey winger fires Roma's revival ... Cengiz Under has scored his fourth goal in three matches and Roma has won at Udinese 2-0 for its third straight win in Serie A. Roma moves into third place, two points ahead of Inter Milan, before Inter's match at Genoa later ... ROME (AP) — The rapid development of 20-year-old Turkey winger Cengiz Under is playing a big role in Roma's revival....