Figure 3.

Long term maintenance of bifunctionality in culture. TIL 5 TCR-transduced cultures were cocultured for 24 hours in a 1:1 ratio with T2
cells pulsed with 5 μg/ml peptide at varying time points beyond transduction. Supernatants
were collected and the amount of interferon-γ released was measured using ELISA. Values
are the average of triplicate wells. Asterisk (*) indicates value was greater than
maximum point on standard curve.