Endotoxin-Induced Release of Colony-Stimulating Factor

Abstract

The introduction of an in vitro technique for cloning granulocyte and macrophage precursor cells by Pluznik and Sachs (1965) and Bradley and Metcalf (1966) provided a new approach to the evaluation of the regulation of granulopoiesis and macrophage formation. Committed precursor cells for granulocyte and macrophage differentiation can proliferate in soft agar cultures to form colonies of granulocytes and/or macrophages (Ichikawa et al., 1966). Colonies arise from single cells termed colony-forming cells (CFC) (Pluznik and Sachs, 1966). Colony formation is wholly dependent upon the constant presence of a stimulatory glycoprotein substance named colony-stimulating factor (CSF).

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