Yeast Transformation Kit—Yeastmaker Carrier DNA

The Yeast Transformation System 2 protocol provides a higher and more reliable transformation efficiency than many other commonly used methods as a result of specially formulated "YPD Plus" recovery medium, which results in more surviving transformed colonies and optimized Yeastmaker Carrier DNA-Salmon sperm DNA, which aids the uptake of plasmid DNA.

YPD Plus Recovery Medium

Following transformation, yeast cells are incubated in YPD Plus Liquid Medium—a proprietary formulation that enhances the recovery of yeast from heat shock. Using this protocol with yeast strains Y2HGold, AH109, or Y187, we typically obtain greater than 3 x 105 transformants per µg of plasmid DNA.

Optimized Carrier DNA

Features

High efficiency protocol for small-scale or library-scale transformations

YPD Plus formulation promotes higher number of transformants

Optimized carrier DNA

Applications

Preparation and transformation of competent yeast cells

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.