Cholesterol Metabolism TF Activation Profiling Plate Array

Characterization

Description:

Cholesterol is an essential component of cell membranes to ensure cell viability and cell growth, and also serves as a precursor for steroids and bile acids. The changes in cholesterol level could lead to abnormal biological process.The elevated levels have been reported to be associated with several diseases, including cardiovascular disease, heart attack, stroke and Huntington's disease. It has been widely reported that cholesterol homeostasis and metabolism are regulated by a group of transcription factors. The SREBP-2 transcription factor, for instance, is responsible for regulating cholesterol synthesis in the liver. LXR, on the other hand, serves to monitor cholesterol levels and interacts with oxysterols to drive the disposal of excess cholesterol. FXR detects intracellular levels of bile acids and works to prevent their over-accumulation. Profiling the regulation and activation patterns of these associated transcription factors is crucial to further understanding and unraveling the intricate cholesterol metabolism pathway.

Signosis have developed and produced various transcription factor plate arrays to help you detect specific activation of the many transcription factors involved in cholesterol metabolism, including SREBP, LXR, FXR, ATF4, CREB, FOXO, HNF, C/EBP, PPAR, COUP-TF, NUR77, EGR, GATA, NFkB and CHOP.

Applicable Grid:

List of Applicable TFs:

1

2

3

4

5

6

7

8

9

10

11

12

A

SREBP

PPAR

SREBP

PPAR

SREBP

PPAR

SREBP

PPAR

SREBP

PPAR

SREBP

PPAR

B

LXR

Coup-TF

LXR

Coup-TF

LXR

Coup-TF

LXR

Coup-TF

LXR

Coup-TF

LXR

Coup-TF

C

FXR

NUR77

FXR

NUR77

FXR

NUR77

FXR

NUR77

FXR

NUR77

FXR

NUR77

D

ATF4

EGR

ATF4

EGR

ATF4

EGR

ATF4

EGR

ATF4

EGR

ATF4

EGR

E

CREB

GATA

CREB

GATA

CREB

GATA

CREB

GATA

CREB

GATA

CREB

GATA

F

FOXO

NFkB

FOXO

NFkB

FOXO

NFkB

FOXO

NFkB

FOXO

NFkB

FOXO

NFkB

G

HNF

CHOP

HNF

CHOP

HNF

CHOP

HNF

CHOP

HNF

CHOP

HNF

CHOP

H

C/EBP

Blank

C/EBP

Blank

C/EBP

Blank

C/EBP

Blank

C/EBP

Blank

C/EBP

Blank

Principle

Signosis’ TF Activation Profiling Arrays are used for monitoring the activities of multiple TFs simultaneously. In the technology, a series of biotin-labeled probes are made based on the consensus sequences of TF DNA-binding sites. When the probe mixtures incubate with nuclear extracts, individual probes will find their corresponding TFs and form TF/probe complexes, which can be easily separated from free probes through a simple spin column purification. The bound probes are then detached from the complexes and analyzed through hybridization with a plate, which each well is specifically pre-coated with complementary sequences of the probes.