LOS ANGELES (AP) — NASA’s Curiosity rover hasn’t discovered any signs of methane in the atmosphere of Mars, a finding that does not bode well for the possibility that microbes capable of producing the gas could be living below the planet’s surface, scientists said Thursday.

Since landing in Gale Crater last year, the car-size rover has gulped Mars air and scanned it with a tiny laser in search of methane. On Earth, most of the gas is a byproduct of life, spewed when animals digest or plants decay.

Curiosity lacks the tools to directly hunt for simple life, past or present. But scientists had high hopes that the rover would inhale methane after orbiting spacecraft and Earth-based telescopes detected plumes of the gas several years ago.

“If you had microbial life somewhere on Mars that was really healthy and cranking away, you might see some of the signatures of that in the atmosphere,” said mission scientist Paul Mahaffy of NASA’s Goddard Space Flight Center.

During Curiosity’s first eight months on the red planet, it sniffed the air during the day and at night as the season changed from spring to summer.

“Every time we looked, we never saw it,” said Christopher Webster, of NASA’s Jet Propulsion Laboratory, who led the research published online in the journal Science.

Webster said while the result was “disappointing in many ways,” the hunt for the elusive gas continues. While methane is linked to living things, it can also be made by non-biological processes.

Michael Mumma of NASA’s Goddard Space Flight Center previously noticed a mysterious belch of methane from three regions in Mars’ western hemisphere. Mumma, who had no role in the latest study, said he stood by his observations.

Earlier this month, Curiosity reached its first rest stop in its long trek toward Mount Sharp, a mountain rising from Gale Crater near the equator. The rover will take monthly readings of the Martian atmosphere during the road trip, expected to last almost a year.

Curiosity previously found evidence of an ancient environment that could have once been suitable for microscopic life. Scientists still hope to uncover signs of organic molecules, considered the chemical building blocks of life, at the base of Mount Sharp.