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Proceedings of the National Academy of Sciences of the United States of America

Description:

PNAS is the world's most-cited multidisciplinary scientific serial.
It publishes high-impact research reports, commentaries, perspectives, reviews,
colloquium papers, and actions of the Academy. In accordance with the guiding
principles established by George Ellery Hale in 1914, PNAS publishes
brief first announcements of Academy Members' and Foreign Associates' more
important contributions to research and of work that appears to a Member to
be of particular importance.

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Abstract

Enhanced transductional recombination between specialized transducing phage λ plac5 and plasmid F42lac depends on cis- and trans-acting factors. By constructing a series of recombinant molecules, the cis-acting site required for enhanced recombination has been identified as oriT, the origin of conjugational transfer of the F sex factor of Escherichia coli. The trans-acting factors are located in the promoter-proximal and the promoter-distal regions of the traY-to-traZ operon.