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Bioinformatics and Systems Biology

3D Reconstruction Via Stereoscopy for the Study of Mosquito Swarms

Here you will find materials related to the stereoscopic analysis of video footage employed by Manoukis et al. (2009) to study swarms of Anopheles gambiae.

Introduction

Male mosquitoes swarm to find mates, but the characteristics of these swarms have not often been quantitatively analyzed in the field. This site includes information on a simple method used recently to localize individual Anopheles gambiae in a swarm via stereoscopic image analysis of video footage swarms recorded in Doneguebougou, Mali. The methods described here represent a streamlining of possibly the most basic method for localizing objects in space, in the hope that other researchers will be able to apply the method for studying fundamental questions about flying insects or other taxa.

To understand the method and its limitations, read "Description of a Method for Localizing Swarming Mosquitoes in 3D Space" and the original Manoukis et al. paper, "Structure and Dynamics of Male Swarms of Anopheles gambiae."

Software

Manoukis et al. used various third-party programs to analyze our data. These are cited in the document below ("Description of a Method for Localizing Swarming Mosquitoes in 3D Space"), including URLs for download. The researchers also developed two programs to facilitate insect localization:

An R function to calculate positions from stereo measurements, called calcxyz.R

A convenience plugin for ImageJ, which may be used to gather these data easily (called StereoPointPicker, a small modification of PointPicker by Philippe Thevenaz