Application Information: CD32a antibody [6H7]

We recommend the following starting dilutions:
For WB: Use at a dilution of 1:1000.
For IHC-P: Use at a dilution of 1:50.
For IP: Use at a concentration of 2ug/500ul.
Optimal dilutions should be determined experimentally by the end user.

Form Supplied

Liquid

Purification

Protein A/G purified

Specifications: CD32a antibody [6H7]

Full Name

Fc fragment of IgG, low affinity IIa, receptor (CD32)

Background

Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A .