Objective To observe the efficacy of ulinastatin combined with gamma globulin in treating patients with acute purulent peritonitis.Methods 70 cases diagnosed with acute purulent peritonitis from March 2013 to March 2015 were randomly divided into observation group and control group,35 cases in each group. The control group received conventional treatment and observation group received ulinastatin combined with gamma globulin treatment. The related indicators were followed up and recorded in two groups post-treatment. Results The endotoxin content post-treatment in observation group was lower than control group[( 626. 9 ± 130. 5) vs.( 867. 4 ± 124. 2) pg / mL,P < 0. 05]. The effective rate in observation group was higher than control group( 97. 14% vs. 77. 14%; χ~2= 7. 467,P < 0. 05). Conclusion Ulinastatin combined with gamma globulin has the exact efficacy in treating patients with acute purulent peritonitis whose efficacy is better than conventional treatment.