gel electrophoresis

The aim of this lab is to determine the sex of the rainbow lorikeets through agar gel electrophoresis. Gel is 2% agar and stained with GelGreen that has been sitting on my desk room temperature since last test (which was successful). I will run a ladder as well to make sure that the gel is still showing luminescence.

Gel ran at 100V (intially started at 300V for a few seconds and then pansied out) and 60mA. Immediate illumination could be seen in the well with the ladder. Gel is running and I can see the ladder but nothing else. Fuck. Looks like I am going back to PCR. After 300bp had reached centre, still no light from samples. Maybe run a single lorikeet sample through PCR and then check on a gel.