Protocols for administering subcutaneous silastic hormone implants. Includes descriptions of the surgical method, including protocols for using inhaled anesthetics, and the non-surgical trochar implantation method.

Protocol for testosterone EIA, based on the protocol with the kit from Assay Designs. Similar to the full protocol, but modified so that radiolabeled testosterone is only added to the standards, not the samples.

A spreadsheet for calculating testosterone concentrations based on enzyme immunoassay results. Has spaces for the radiation counts per minute to be entered to calculate recovery rates, the concentration from the plate ...

Protocol for administering a GnRH challenge to a songbird. This procedure injects a bird with a known concentration of gonadotropin releasing hormone and measures the amount of testosterone produced in response.

Protocol for PCR of extracted avian DNA. Includes directions for PCR to amplify microsatellite loci (used for paternity analysis) and for PCR to amplify sex-specific loci (used for determining sample sex). Includes ...