†This article is part of the Special Issue dedicated to the memory of Elsa Abuin.

Abstract

The role of recombinant Type-I human collagen in the free form or forming AgNP@collagen on the photophysical and photochemical behavior of rose Bengal was analyzed. The formation of dye aggregates on the protein surface was experimentally observed and corroborated by docking calculations. The formation of such aggregates is believed to change the main oxidative mechanism from Type-II (singlet oxygen) to Type-I (free radical) photosensitization. Remarkably, the presence of AgNP in the form of AgNP@collagen altered the dynamics of dye triplet deactivation, effectively preventing the dye degradation and reducing the extent of protein crosslinked. Both crosslinked rHC and AgNP@collagen were able to support fibroblasts proliferation, but only the material containing silver was resistant to S. epidermidis infection.

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Supporting Information

Actual picture and power spectrum for the 525 nm LED employed in the samples irradiations in this study. Changes in the Zeta potential values, mV, for 2.5 μm Type-I collagen in the presence of different concentrations of rose Bengal. Variation on the Rpn value of 2.5 μm rHC upon addition of different concentrations of rose Bengal up to 20 μm. Docking simulation for the interaction of different numbers of RB molecules, as indicated by the n number with a Type-I collagen-like protein. LDS electrophoresis for pure human recombinant Type-I collagen, rHC, and AgNP@collagen, AgNP, before irradiation of the samples under different experimental conditions are available free charged on DOI:10.1562/2006-10.1111/php.12119.s1.

Figure S1. Left: Actual picture for the 525 nm LED employed for the irradiations in this study. The system was composed by an LZ4-00G110 LED unit (LedEngin, Inc.) mounted on a PAR25 LED Cooler 32W Synjet that helps to dissipate the heating produced by the LED. Right: Power spectrum of the LED measured at the exact same distance of the center of the cuvette shown in the left picture.

Figure S2. Top: Zeta potential values, mV, for 2.5 μm Type-I collagen in the presence of different concentrations of rose Bengal. P value was calculated from Student's t-test statistic analysis. Bottom: Variation on the Rpn value of 2.5 μm rHC upon addition of different concentrations of rose Bengal up to 20 μm.

Figure S3. Docking simulation for the interaction of different numbers of RB molecules, as indicated by the n number with a Type-I collagen-like protein (further information is included in the experimental main text).

Figure S4. LDS electrophoresis for pure human recombinant Type-I collagen, rHC, and AgNP@collagen, AgNP, before irradiation of the samples under different experimental conditions (see main text for further details).

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