Light-sheet fluorescence microscopy has become the method of choice for non-invasive imaging of a variety of live and cleared biological samples ranging from subcellular structures to cells, tissues and whole embryos, e.g. Drosophila and Zebrafish.

Among other features, the significantly reduced photodamage effects and the very high acquisition speed make light-sheet fluorescence microscopy stand out.

The MuVi SPIM, the InVi SPIM, the QuVi SPIM, the LCS SPIM and the TruLive3D Imager, as well as the solutions for photomanipulation, have been built up around your sample to meet the requirements of your application for fixed or cleared samples. Take a look at the different examples.

Light-sheet fluorescence microscopy enables the observation of events in real time for several days.

The MuVi SPIM was conceived for gentle, large specimen imaging. It features four orthogonal views of the sample without the need for rotation. The low magnification, high NA objective lenses enable fast, high-resolution, large field-of-view imaging of entire embryos as well as tissue and organ development tracking.

Drosophila Embryo Development

Transgenic line expressing His2Av-mCherry as fluorescent nuclear reporter. The fruit fly embryo was imaged for almost one complete day (4 × 200 slices every 30 seconds). Imaged on the MuVi SPIM.

Zebrafish Development

Zebrafish imaged on the MuVi SPIM. Stitched from 5 stacks in Imaris, each 340 slices, 16 hour/10min. Fish growth can be observed.

Courtesy of:Prof. Jingxia LiuHuazhong Agricultural UniversityChina

Drosophila Egg chambers

Drosophila ovariole stained with phalloidin to label actin (red) found along membranes and in the germline ring canals, DAPI (blue) to show the nuclei and a somatic ring canal marker (green) to label the ring canals in the epithelium. Imaged on the MuVi SPIM.

Drosophila Embryo

Vascular Development in Zebrafish

From left to right: the video shows a beating Zebrafish heart imaged at 50 frames/sec, followed by Zebrafish blood vessels (magenta) and red blood cells (yellow) and Zebrafish blood flow imaged at 50 frames/sec. Imaged on the MuVi SPIM.

Organoid research has been established as an essential tool for studies in cancer research, drug discovery and regenerative medicine.

The InVi SPIM singles out in the gentle handling of the most delicate samples and the minimization of required specimen medium. This is achieved by the incubation capacities and the V-shaped sample chamber. If high-throughput is also desirable, the QuVi SPIM or the TruLive3D Imager

Pancreatic Spheres

hESC-derived pancreatic spheres. Imaging on the TruLive3D Imager enables collecting information of several samples in one experiment. Visualization: Imaris (Bitplane)

Courtesy of:Yung Hae KimGraphin-Botton Group, MPI-CBGDresden, Germany

Fixed Astrocyte Spheroid

Spheroid stained with anti-GFAP (Alexa 488) to label astrocytes and anti-Neurofilament200 (Alexa555) to label neurons. Imaged on the InVi SPIM.

3D Cell Culture

3D Imaging of an Spheroid

Spheroid labeled with EGFP and mRFP imaged on the InVi SPIM Lattice Pro. Three illumination patterns were tested for each label: Gaussian beams, Bessel beams and optical lattices. The optical lattices gave the best results for the EGFP labelling, while the Gaussian beam was optimal for the mRFP labelling.

Courtesy of:Martin StöcklUniversity of KonstanzGermany

Colonies of Mouse Embryonic Stem Cells

Colonies of mouse embryonic stem cells stably expressing H2B-mCherry and IRFP670 with a membrane-targeting signal. Imaged on the InVi SPIM.

The application of light-sheet fluorescence microscopy for plant research has allowed in vivo studies of plant cell and tissue biology.

In combination with other approaches, imaging of cell morphology, migration and organization, as well as tissue structure enable a better understanding of function and dynamics. The MuVi SPIM, but also the InVi SPIM provide the relevant tools for the research on the field.

The InVi SPIM enables gentle handling of the most delicate samples and the use of small volumes of mounting media. The TruLive3D Imager further enables multi-sample imaging. In both cases, precise control of CO2, O2, temperature and humidity provides close-to-natural conditions to the sample. The QuVi SPIM further brings high-throughput imaging capabilities.

HeLa Cell Culture

Light-sheet fluorescence microscopy has enabled imaging of the central nervous system including large networks of neurons, and even whole cleared brains (e.g. mouse).

Both the MuVi SPIM LS and the InVi SPIM are suited for in-vivo imaging of nervous system development in small embryos like Drosophila and Zebrafish, while the MuVi SPIM CS was conceived to image large cleared samples.