In article <alena.5.0010294D at scientia.up.ac.za>, alena at scientia.up.ac.za
says...
>>I want to quantify cytokines secreted by T cells upon infection of mice,
> but I don't know the easiest way to do this. Is it possible to do this by a
>quantitative PCR with the right primers (for instance for IL-2 or IFN)?
>Anne Lenaerts (from Belgium)
>alena at scientia.up.ac.za
No, RT-PCR measures RNA levels. For secreted cytokines I believe
the "conventional" methods are based upon sandwich ELISA.
A company called Cytimmune Sciences visited recently and were pushing
an alternative to the sandwich method which was based on competetion
using biotinylated cytokines. I guess there are plenty more systems out
there.
[No affiliation with Cytimmune...- not even a customer]
Bernard
Bernard Murray, Ph.D.
bernard at elsie.nci.nih.gov (National Cancer Institute, NIH, Bethesda MD, USA)