Description of two new species of freshwater amphipods from La Gomera (Canary Islands), both found in the higher parts of the island: Chaetogammarus chaetocerus n. sp. and Rhipidogammarus gomeranus n. sp. Both species have distinct Afro- Iberian relationships.

Description of two new species of freshwater amphipods from La Gomera (Canary Islands), both found in the higher parts of the island: Chaetogammarus chaetocerus n. sp. and Rhipidogammarus gomeranus n. sp. Both species have distinct Afro- Iberian relationships. Minimize

http://epub.ub.uni-muenchen.de/6315/1/6315.pdf ; Kellerer, Albrecht M. und Stock, Gabriele und Chmelevsky, Danielle und Barclay, David (1991): Exploratory analysis - a visualization of the data from RERF by non-parametric methods. Workshop held at Schloss Elmau on 4 - 8th March 1991 under the joint sponsorship of the US Department of Energy,...

16th SETAC GLB (Society of Environmental Toxicology and Chemistry German Language Branch) Annual meeting held under the main theme “EcoTOXICOlogy and Environmental CHEMISTRY: crossing borders” from 18th to 20th September 2011 at Landau

16th SETAC GLB (Society of Environmental Toxicology and Chemistry German Language Branch) Annual meeting held under the main theme “EcoTOXICOlogy and Environmental CHEMISTRY: crossing borders” from 18th to 20th September 2011 at Landau

Inhibition of deacetylases represents a new treatment option for human cancer diseases. We applied the novel and potent pan-deacetylase inhibitor panobinostat (LBH589) to human hepatocellular carcinoma models and investigated by which pathways tumor cell survival is influenced.HepG2 (p53wt) and Hep3B (p53null) responded to panobinostat treatment...

Inhibition of deacetylases represents a new treatment option for human cancer diseases. We applied the novel and potent pan-deacetylase inhibitor panobinostat (LBH589) to human hepatocellular carcinoma models and investigated by which pathways tumor cell survival is influenced.HepG2 (p53wt) and Hep3B (p53null) responded to panobinostat treatment with a reduction of cell proliferation and a significant increase in apoptotic cell death at low micromolar concentrations. Apoptosis was neither mediated by the extrinsic nor the intrinsic pathway but quantitative RT-PCR showed an upregulation of CHOP, a marker of the unfolded protein response and endoplasmic reticulum stress with subsequent activation of caspase 12. Dependent on the p53 status, a transcriptional upregulation of p21cip1/waf1, an increased phosphorylation of H2AX, and an activation of the MAPK pathway were observed. In a subcutaneous xenograft model, daily i.p. injections of 10 mg/kg panobinostat lead to a significant growth delay with prolonged overall survival, mediated by reduced tumor cell proliferation, increased apoptosis and reduced angiogenesis in tumor xenografts. Panobinostat increased the acetylation of histones H3 and H4.Panobinostat is a well tolerated new treatment option for HCC that activates alternative pathways of apoptosis, also in p53-deficient tumors. Minimize

Introduction and Aims: Interstitial fibrosis and tubular atrophy (IF/TA) are the major mechanisms of long-term renal graft loss, and no treatment can reverse fibrosis once it has developped. Early markers are needed to assess patients at risk of IF/TA and invent early interventions. Our previous studies showed that tubular epithelial phenotype ...

Introduction and Aims: Interstitial fibrosis and tubular atrophy (IF/TA) are the major mechanisms of long-term renal graft loss, and no treatment can reverse fibrosis once it has developped. Early markers are needed to assess patients at risk of IF/TA and invent early interventions. Our previous studies showed that tubular epithelial phenotype changes (EPC), reminiscent of the epithelial to mesenchymal transition (EMT) process, constitute an early biomarker that predicts late renal graft fibrosis and loss of function. However, the mechanisms that link EPC and tissue fibrosis remain to be identified. The hypothesis that interstitial fibroblasts may be derived from tubular epithelial cells is vividly disputed. In the present study, we wanted to test the hypothesis that activated epithelial cells exhibiting EMT-like changes could, by an autocrine or paracrine signaling mechanism, produce extracellular matrix components and thereby directly contribute to fibrogenesis, even within renal tubules. Methods: Using immunohistochemistry we detected the presence in tubular cells of a) Connective Tissue Growth Factor (CTGF), a key mediator of fibrogenesis, b) HSP47, an important chaperon protein for collagen synthesis, and 3) laminin, a major component of the basement membranes, in 93 renal graft biopsies from 77 patients. We then analyzed the association between these three molecules and the intensity of two EPC/EMT markers (the de novo expression of vimentin and the translocation of β catenin into the cytoplasm). Results: We observed an up-regulated production of CTGF, hsp47, and laminin in the tubular epithelial cells of most renal transplants. These pro-fibrotic molecules colocalized in tubules showing EPC markers. In particular, the score of expression of the mesenchymal cell marker vimentin was significantly correlated with that of CTGF (r=0.79, p<0.0001), HSP47 (r=0.89, p<0.0001) and laminin (r=0.84, p<0.0001). In addition, the expression of vimentin in renal grafts was significantly correlated with the estimated glomerular filtration rate (r=-0.61, p<0.0001), as well as with the proteinuria (r=0.42, p=0.0006) at the moment of the biopsy. Conclusions: Our present study demonstrates that the phenoytpic switch that epithelial cells undergo in the context of renal transplantation results in the aberrant production of extracellular matrix proteins by tubular cells themselves, thus directly contributing to graft fibrogenesis, and also in the production of pro-fibrotic growth factors such as CTGF, which could activate myofibroblast from a distance, in a paracrine fashion, and similarly accelerate graft fibrogenesis. Overall, the fundamental question of whether or not EMT+ cells do migrate and swell the population of interstitial fibrosis should not eclipse the strong predictive value of EPC with respect to progression of fibrosis. Minimize