Hi,
I synthetised cDNA starting from mRNA from plant sources. After addition
of Eco RI/Not I adaptors and insertion into lgt 11 phage I only become
blue plaques. The problem is probably the addition of the
EcoRI/Not I adaptors or the insertion into the lgt 11 phage. However, I
strictly followed the instructions of the kit I used (TimeSaver cDNA
synthesis kit). Do you have any suggestions to become recombinant plaques
?
Thanks,
Els Van Geldre