The anime will be a 22-episode season that will cover the storyline of the live-action version's first two seasons. The first volume will ship on January 12 with the first two episodes. On February 2, the "Box 1" set will ship with episodes 3 to 12. On April 6, the "Box 2" set will ship with episodes 13 to 22. (There will be a different release schedule for the rental version of the project.)

The anime project will not only remake the best episodes from the live-action version, but also depict original episodes not seen in the live-action version. Those original episodes will include prologues of the Winchester brothers' childhood, anime-only enemies, and episodes featuring secondary characters from the live-action version.