FluoTag®-Q anti-RFP

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Fluorophore

FluoTag®-Q anti-RFP is derived from an in-house developed single-domain antibody (sdAb) recognizing the most common red fluorescent proteins like mRFP, mCherry, DsRed and other DsRed derivatives with high affinity and specificity. For a complete specificity chart see the Product Reactivity Chart. In our FluoTag-Q series, each fluorophore is coupled to exactly one FluoTag, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

Specificity: Recognizes most common red fluorescent proteins like mRFP, mCherry, dsRed, mScarlet-i and tdTomato in their native conformation. It does not cross-react with GFP or mTagBFP derivatives. For more detailed information see the Product Reactivity Chart.Concentration: 5 µM protein, 5 µM fluorophoreRecommended dilution for IF/ ICC: 1:1000