Ok, the other stickie up there is a less than comprehensive list of Algae Culture and Identification sites.viewtopic.php?f=145&t=124I have tried in the past to update this list but have let it go in the past few months.If anyone finds other sites, Let me know and I will add them.

I also need to organize that list into appropriate categories.John

"The exact contrary of what is generally believed is often the truth" Jean De La Bruyère (1645-1696)

Thanks, John, but I've clicked on all of the links in that sticky, and most of them are now broken. Once again (hawklover, are you listening?) I'd be willing to work with others on organizing this info in a useful, approachable format, FWIW.

JimWelsh wrote:Thanks, John, but I've clicked on all of the links in that sticky, and most of them are now broken. Once again (hawklover, are you listening?) I'd be willing to work with others on organizing this info in a useful, approachable format, FWIW.

Thanks Jim.Most of those sites still exist. I'll try to fix them today.But this points out a problem with working with links to other sites and internet information. Sometimes the linked site just disappears. We really should try to consolidate the How too information and tables here if possible.John

"The exact contrary of what is generally believed is often the truth" Jean De La Bruyère (1645-1696)

Jim, I am here and still listening! If I can be of any help at all, please let me know. I am a hands on learner and have to read things over and over unless I have it right in front of me. Let me know what I can do, I would love to help!

TetraselmisSalinity 25-35 pptTemp 19-21°C"Variations in salinity and in nutrient concentration had a greater effect on the final biomass than on the growth velocity. The total protein of the culture and protein per cell increased when the salinity increased for a given nutrient concentration."

I use 2L Erlenmeyer flasks. This is better than soda bottles because you can sterilize them (fill with boiling salt water). I buy them from Thermo Fisher, with caps. I dril a small hole in a cap and put in a 1 ml sterile pipette with sterile cotton plug. I also boil water. Before I started doing this, I could not propagate my phyto for more than couple months. Now it seems to be growing indefinitely.

Hey Luis I started another thread on this but should I go with the Tahitian iso over the other iso strains? What's special about this strain. I'm trying to culture parvo, a tonsa, and pseudo. I only want to culture one algae only and want something stable(as stable as possible anyway). I know about keeping stuff sterile and everything like that. Sanitation won't bother me. What about Tetra? Feel free to answer on either thread or both. Sorry bout asking twice just want your expertise on this.

Luis A M wrote: Stock cultures are kept pure and possibly sterile in flasks. Each flask is filled with 60 ml of medium, cotton stopped and sterilized in the microwave oven. When cool, 10 ml of a thriving culture is seeded with a sterile pipette.

Hi Luis, how long are you microwaving these 60ml stock cultures? Is it the full typical 10mins? I’d imagine this would bring them to boil if done separately.

Hi Luis, yes I am. Last night I tried 1200ml of water in the Pyrex and had three 100ml ehrlenmeyers cotton topped each filled with 100ml of culture water and during the 2nd minute of the 4min stint they started to boil over. I assume it’s the length of radiation exposure (10mins in total) that is important here? Wondering how important the 4min block is and whether this can split into two shorter 2min blocks with a break in between.