The longevity of resting sporangia of synchytrium endobioticum in soil

Resting sporangia of the potato wart disease pathogen S. endobioticum which persist in soil can be extracted from soil by a wet-sieving and chloroform centrifugation method and counted. Soils from the sites of old wart outbreaks were checked for resting sporangia. Sampling procedures, patterns and errors are being assessed.