Master of Wisdom

Are You a Master of Wisdom?

A true Pipetting Ninja knows how to handle problematic liquids wisely. Do you dare to call yourself a Master of Wisdom? Master our challenge and find out. Once you have answered all questions correctly you can print your personalized certificate as proof of your expertise. Have fun and challenge your colleagues.

1. Which pipetting technique should be applied for glycerol? *

Cutting the pipette tip

Reverse and slow pipetting

No special technique is needed

2. What happens when ethanol is pipetted without pre-wetting the pipette tip? *

The ethanol drips out of the pipette tip

Nothing special happens

The ethanol cannot be dispensed

3. Is aerosol formation more likely with warm liquids? *

No, the risk is the same

Yes, because warm liquids evaporate more than cold or room temperature liquids

Only if the liquid is infectious

4. How should volatile liquids (ethanol, acetone) be pipetted? *

Reverse pipetting and 2-3 times pre-wetting of the pipette tip

No special technique is necessary

Pipetting by mouth is sufficient

5. How can you protect a pipette from inner contamination? *

Use filter tips and a cautious working approach

Spray disinfectant into the pipette

You cannot protect a pipette from inner contamination

6. What do you have to do when pipetting liquids with a lower or higher density than water? *

Adjust the pipette to the appropriate density

Apply double step pipetting

Nothing special needs to be considered

7. Which instrument can be used for problematic liquids without further consideration? *

Direct displacement pipette/dispenser

Air cushion pipette

Serological pipets

8. Which effect is visible with high protein containing liquids like BSA or cell culture medium? *

The blow-out can lead to foam formation

Nothing special happens

The liquid sticks to the outside of the pipette tip

9. What can happen when pipetting detergents? *

Liquid will remain in the pipette tip and sticks to the outside of the pipette tip