anti-Cytochrome c Antibody

Details

Target of this antibody:

Cytochrome c

Alternative names of antibody target:

CYCS, CYC

Background information:

Cytochrome c (m.w. 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases.

This antibody has been succesfully tested for use in following methods:

Western blot

Recommended usage:

Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Rat kidney lysate (Cat.# 2702-100) can be used as a positive control. The cytochrome c antibody detects the 12 kDa cytochrome c from rat samples.

Storage information:

Shelf time: 12 months in temp.: -20 deg.C

Shipping conditions:

gel pack

How to handle this antibody:

The antibody solution should be gently mixed before use.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Properties

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.