The AlphaLISA® Human cardiac Troponin I Detection Kit is designed for detection and quantitation of human cardiac Troponin I in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

The kit was designed to equally detect free Troponin I, Troponin IC complex, or Troponin ITC complex. Troponin I is detected using two mouse monoclonal antibodies: clone numbers M18 and 267.

Formats:

Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

No-wash steps, no separation steps

ELISA alternative technology

Sensitive detection

Broad sample compatibility

Small sample volume

Results in less than 3 hours

Half the time of an ELISA assay

Troponin is one of the main proteins involved in muscle contraction and consists of three subunits: troponin C, involved in calcium binding; troponin I, the inhibitory subunit; and Troponin T, the tropomyosin binding subunit. Cardiac TnI (cTnI) is a well known serum biomarker of cardiac muscle injury, especially of myocardial infarction. Once released in the blood stream, cTnI is mainly in the binary complex of cTnI and cTnC (42 kDa), but also is present as the free form (24 kDa) and cTnI-cTnT-cTnC or ITC complex (78 kDa). Also, it has been found that healthy subjects having cTnI blood concentrations over the 99th percentile are at a higher risk of cardiovascular disease.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.