Jonathan Zmuda is a Director of Cell Biology at Thermo Fisher Scientific in Frederick, Maryland. Jon leads a team dedicated to discovering and developing new technologies and products useful for cell biology applications, including transient protein expression, advanced cell culture, and rare-cell analysis. He received his Ph.D. in Cell Biology from the University of Maryland, College Park.

Abstract:

The ExpiCHO™ transient expression system offers a turnkey solution for generating high-titer recombinant proteins for therapeutic drug development, reagent generation, as well as an alternative platform for proteins that express poorly in 293-based systems. Because of the significant unmet need for high titer transient CHO protein production, the ExpiCHO system has become a key part of the transient expression workflow in many companies around the world. In this presentation, we will share with researchers the latest data on the ExpiCHO expression system, tips to help optimize the system for maximal performance and protocols to allow for scalable protein expression from the micro to the macro scale.