On Thu, 10 Jul 1997 08:11:35 GMT, a8803349 at unet.univie.ac.at (Martin
Offterdinger) wrote:
>On 1 Jul 1997 10:00:38 -0700, cxi3 at PSU.EDU (Chris Ingle) wrote:
>>>Hi All!
>>>>Has anyone out there had any success with N. blots and
>>nonradioactively-labeled probes? I have been trying to probe my blot
>>with a DIG-labeled probe, and visualize using chemiluminescence, with no
>>success. My probe is efficiently labeled, but after I hybridize (using
>>Clontech's ExpressHyb), wash, and expose, nothing. Well, except a
>>little bit of a schmear on the blot, but I know my probe is highly
>>specific, so I think that's just background. Our lab is currently
>>waiting to be cleared for radioisotopes, so until then I have to use
>>other means. Any ideas/advice? Please e-mail me directly rather than
>>posting to the newsgroup, if possible.
We've had several people of late calling tech service with this
problem. We routinely use biotin labeled probes, but I inderstand why
you want to use dig (using it for in situs, right?)
Try the CDP star chem substrate from Tropix.