pyMantis

pyMantis Demo

demo

Tools

Primer Search Tool

Primer Library Matching

* 'Primer Library Matching' option in Primer Search Tool performs one to one matching against sub-sequences in a single or group of sequences with a group of primers.
* The primer library is a library of primer files in .TXT format stored in a zipped archive,each primer in the library is aligned against a sequence or a library of sequences of interest for complete or partial matching of primer and each sub-sequence in a sequence which is of primer length.
* The results generated depends on the percentage matching between the primer and sub-sequence which can be given in the '%Matching' field.
* The results constitute Filename of sequence,alignment of primers against the sequence, alignment of primer against complimentary sequence ,the location of primers in sequence and complimentary sequence and three different annealing temparatures
* The required sequence and primer files for this process can be uploaded into the database of 'Primer Search Tool' through 'Upload Primer Files' and 'Upload Sequence File(s)' options respectively.

Single Primer Matching

* 'Single Primer Matching' option in Primer Search Tool performs matching a single primer against sub-sequences in a single or group of sequences.
* The field 'Query Primer' takes a single primer as query against the a sequence or library of sequences of interest for complete or partial matching of query primer and each sub-sequence in a sequence which is of primer length.
* The results generated depends on the percentage matching between the primer and subsequence which can be given in the '%Matching' field.
* The results constitute Filename of sequence,alignment of primers against the sequence, alignment of primer against complimentary sequence ,the location of primers in sequence and complimentary sequence and three different annealing temparatures
* The sequence(s) can be uploaded into the database of 'Primer Search Tool' through 'Upload Sequence File(s)'.