Introduction Most experts agree that in the general population old teenagers and young adults tend to be the most sexually active and therefore at higher risk than some other groups (Curtin, 1996). Making continuous assessment on the degree of awareness and behavioral change on prevention of HIV/AIDS is uninterrupted work. Since this age group is found in high schools, colleges, and University .This research work is interested to assess the sexual behaviors related to the prevention of HIV/AIDS in these education institutions. This study may contribute in initiating concerned policy makers, health workers, teachers, administrators, and parents, NGOs to assess their strategies and strengthen their efforts in order to create better awareness, behavioral change and accessibilities for prevention of HIV/AIDS. It may also strengthen the global efforts on prevention of HIV/AIDS. Objectives-To assess the status of safe sexual behavior in the educational institutions and promote prevention of HIV/AIDS Methodology-Institutions based cross sectional study design was employed. This research work has been carried out by dispatching self administered questionnaires randomly among the students of secondary, preparatory, TVET, CTE, University educational institutions found in central zone of Tigray region, Ethiop[ia. Two sections (departments) were selected randomly from each institution. The collected data was analyzed quantitatively entering in to a computer using SPSS Version 16 using Chi-square, Annova, Sign test. Result More students found, abstinence, females (82.8%), males (72.4%). Sexually active students not found in first primary level. But in both sexes sexually active students found in junior primary level (7th&8th grade), in which males are significantly more than females. In both sexes majority students have started sexual activity in their secondary level (9th &10th). The very interesting thing here is all female students, who have arrived with abstinence to university level continue likewise. But among the male students who have arrived with abstinence only 4.4% of them break, their abstinence in their university life. In both sexes majority of those who have started sexual activity continue likewise, females (85.5%), males (67.4%). Unlike to this half of the male students who have arrived sexually active to university level stopped their sexual activity after their arrival. Majority students in both sexes used to mate only with their friends, females (73.6%), males (71.2%). Unlike to this majority of the sexually active preparatory male students (80%) found to used promiscuous sexual behavior. Similarly about half the female (58.3%) and male (53.8%) university students found to use promiscuous sexual behavior. The other unlike behavior is, majority female students (69.6%) as well as male students (66% were not on using condom regularity. The main reasons given by, female students not to use condom regularly was because of trusting their boyfriends (37.1%), they dislike it (31.4%), their boyfriends, dislike it (25.7%). Likewise the reasons given by male students were, because they trust their girlfriends (44.3%), they dislike it (24.3%), their girlfriends dislike it (22.9%). In both sexes as well as in all level of education, majority students had made HIV/AIDS checkup. And among these 1.6% females and 2% males found HIV/AIDS positive, in which the secondary level students account highest, females (8.6%) and males (9%). However15.5% female and14.6% male students have been found unchecked for HIV/AIDS. The given reason by female students was, lack of available chance (40.7%) confidence (35.6%), and frighten the news might be bad (22%). Likewise the reason by male students was confidence 43.3%, lack of available chance (31.7%) and frighten the news might be e bad (18.3%). Conclusion and recommendation Although majority students found sexual abstinence. considerable number of them had been sexually active. About half of the female and male sexually active university students were found to make promiscuous sexual behavior. In addition to this condition 69.6% the sexually active female students as well as 66% of the sexually active male students were found not, on regular use of condom. These two conditions shows considerable numbers of students are on unsafe sexual behavior. This urges the ministry of education as well as ministry of health and other responsible bodies to intensify the awareness creation programs in students as well as in the rest of the society. According to this research, 84.5% of female as well as 85.4 % of male students found perform HIV checkup. Among these 1.6% females and 2% males found HIV/AIDS positive, This HIV positive condition is not small, so it urges the ministry of education as well as ministry of health and other stake holders to work hard in their formal as well as informal programs.