Description:
Acetylcholine (Ach) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransfrase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.

Applications:Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:Immunohistochemistry: 1:100-1:250Optimal dilutions to be determined by the researcher.

Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type

Isotype

Clone

Grade

Mab

IgG1

2Q28

Ascites

Size

Storage

Shipping

Source

Host

100ul

-20°C

Blue Ice

Rat

Mouse

Concentration:

Not Determined

Immunogen:

Choline acetyltransferase purified from rat brain

Purity:

Ascites

Form

Supplied as a liquid.

Specificity:

Recognizes rat cholinergic neurons in the brain and central nervous system. Reported to crossreact with chicken.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.