Empirical findings show that the practice of meditation has many benefits on human functioning, among which are attention regulation, enchancement of cognitive functions, raised body awareness, adaptive emotion regulation and changes in the perspective of the self. As a result of meditation practice, cognitive, emotional and behavioral responses to the environment become more flexible and less automatic. The aim of the current study was to investigate the impacts which a short-term 10 days meditation practice has on attention regulation, cognitive and emotional flexibility as well as subjective well-being. The study included 63 participants, 53 females and 10 males in the age from 18 to 36, mostly students of the Department of Psychology at the Faculty of Humanities and Social Studies. The results have shown that the levels of mindfulness are higer after compared to before the trainings, with the participants in the meditation condition reporting higher levels in comparison to the control group who was doing progressive muscle relaxation. Both groups have shown increases in efficiency in the networks of alerting and executive control of attention, while there was no change in the network of orienting or on the measure of convergent thinking. On the measures of emotional functioning, results have shown that both groups after the trainings asses negative words more pleasant, while there is no change in the assesment of neutral and positive words. The arousal by verbal stimuli is experienced to a lesser extent after the trainings by both groups, specifically the arousal by negative and positive words while neutral words showed no change. The participants of both groups have also shown an increase in the positive and decrease in the negative mood in the week after the training compared to the week before the training, but there was no change reported in psychological well-being. The level of mindfulness is a good predictor of psychological well-being together with the executive control of attention, while other measures have not show to be valuable predictors. The results of this study show the effect of other factors apart from meditation training that has influenced the participants and their assessments of cognitive and emotional states, and have highlighted the need for further research of the effects of meditation.