Table 2

Relative risks of recurrent disease and death from melanoma according to MCM3 expression

Risk of disease recurrence

Risk of death from melanoma

Univariable

Multivariable

Univariable

Multivariable

n (events)

HR (95%CI)

HR (95%CI)

n (events)

HR (95%CI)

HR (95%CI)

Age

Continuous

255 (47)

1.01 (0.97-1.05)

255 (28)

1.05 (0.99-1.10)

1.08 (1.02-1.14)

Gender

Female

132 (21)

1,00

132 (9)

1,00

Male

123 (26)

1.51 (0.85-2.70)

123 (19)

2.77 (1.25-6.14)

Clark level

II

93 (5)

1,00

93 (2)

1,00

III

103 (21)

4.39 (1.65-11.65)

103 (14)

7.16 (1.63-31.50)

IV-V

51 (21)

9.99 (3.76-26.55)

51 (12)

12.91 (2.89-57.74)

Breslow

Continuous

248 (47)

1.07 (1.03-1.11)

248 (28)

1.08 (1.03-1.13)

Subtype

SSM, LMM, Other

195 (22)

1,00

195 (11)

1,00

1.00

Nodular

53 (24)

5.63 (3.15-10.08)

53 (16)

7.19 (3.32-15.57)

5.51 (2.39-12.71)

Ulceration

No

214 (30)

1,00

214 (18)

1,00

Yes

35 (16)

5.81 (3.13-10.80)

35 (9)

5.73 (2.59-12.97)

Lymphocytic

infiltrate

0-1

72 (20)

1,00

72 (11)

1,00

2-3

176 (26)

0.43 (0.24-0.78)

176 (16)

0.50 (0.23-1.08)

Clinical stage

I

179 (12)

1,00

1,00

179 (7)

1,00

II-IV

28 (13)

15.02 (6.39-35.30)

12.03 (4.49-32.28)

28 (8)

14.09 (4.82-41.18)

Mitotic count

<1/mm2

131 (7)

1,00

131 (3)

1,00

> = 1/mm2

122 (40)

7.96 (3.56-17.80)

122 (25)

11.25 (3.39-37.36)

Vascular invasion

No

232 (35)

1,00

1,00

232 (18)

1,00

1.00

Yes

14 (11)

9.25 (1.67-7.60)

3.56 (2.35-10.92)

14 (9)

11.29 (5.05-25.25)

8.26 (3.30-20.67)

Ki67 expression

Low (<=25 %)

159 (28)

1,00

159 (16)

1,00

High (>25 %)

47 (16)

2.46 (1.33-4.56)

47 (10)

2.64 (1.20-5.83)

RBM3 expression

Low

95 (24)

1.00

95 (17)

1.00

1.00

High

120 (20)

0.50 (0.27-0.91)

120(9)

0.29 (0.13-0.66)

0.30 (0.14-0.71)

MCM3 expression

Low (N ≤ S3)

133 (11)

1.00

1.00

133 (6)

1.00

1.00

High (NS > 3)

91 (34)

5.62 (2.83-11.13)

5.01 (0.33-10.79)

91 (21)

6.03 (2.42-15.02)

4.96 (1.77-13.87)

Low RBM3 expression corresponds to a nuclear staining intensity of negative to moderate
and high expression corresponds to strong staining as previously described [12]. The number of cases in the multivariable analysis is equal to the number of cases
evaluated for MCM3 expression (n = 224).