Cardiolipin IgG EULISA - Discontinued

The EULISA Cardiolipin IgG is a fast and reliable ELISA test kit for In Vitro diagnosis of patients with anti-phospholipid syndrome (APS), an autoimmune disorder characterised by thrombosis in both arteries and veins.

Cardiolipin (CL) is the most common, negatively charged, acid phospholipid. Autoantibodies correlated with APS are directed not only against CL and similar phospholipides but also against phospholipid/protein complexes. ß2-glycoprotein 1 (ß2-GP-1; = apolipoprotein H) has been identified as such a natural and essential co-antigen for CL-autoantibodies. The occurrence of CL/ß2-GP-1 autoantibodies is associated with a tendency towards thrombosis. Besides this diagnostic significance, these antibodies are believed to be directly involved in the pathogenesis of APS.

Technical information

The EULISA Cardiolipin IgG is intended for the quantitative or qualitative determination of IgG antibodies in human serum, directed against CL/ß2-GP-1. The immobilised antigen is a combination of CL, isolated from bovine heart, and a highly purified preparation of native ß2-GP-1. The test is fast (incubation time 30 - 30 - 30 minutes) and flexible (break apart wells, ready-to-use reagents). Six calibrators allow quantitative measurements; a negative and a positive control are used to check assay performance.

Kit components and storage of reagents

Contents of the kit

1 microwell plate, coated with CL/ß2-GP-1 and hermetically packed in a foil laminate pouch together with a desiccant bag.