Anti-Growth Differentiation Factor 9 Clone 72B

Description

GDF9 is plays a vital role in ovarian folliculogenesis, follicle development and fertility. Clone 72B can be used in assays to detect GDF9 expression and help diagnose ovarian disorders.(Gilchrist, R.B. et al.), (Simpson, C et al.)

Specifications

Form in stock: IgG, purified – 1.0 mg/mL. Also available as unpurified supernatant.Host: RatSpecificity: N-terminal of GDF9, corresponding to sequence KKPLGPASFNLSEYFC.Human Histology positive control: OvaryFusion partner: Spleen cells from immunised outbred rats were fused with cells of the mouse NS0 myeloma cell line.Storage: Store at +4°C or -20°C. Avoid repeated freezing and thawing.Shelf life: 18 months from date of dispatch.Regulatory/ Restrictions: For research and commercial purposes.

Clone 53/1 used to detect GDF9 expression by ELISA

Image caption: GDF9 ELISA. A GDF9 ELISA was developed to measure the amount of GDF9 in HEK-293T conditioned medium. Recombinant mouse GDF9 (●) was used as a standard, and the specificity of the assay was assessed using a range of TGF-β family members; wild-type human GDF9 (♦), human GDF9 L40V (◊), human BMP15 (□), human activin A (○), and human TGF-β3 (▾). Dilutions of concentrated media from cells transfected with empty vector, pcDNA3.1 (r), were included as controls. The ELISA has a specificity of less than 0.1%, with a sensitivity of 0.2 ng/mL. Values represent mean ± SEM in duplicate, from a representative experiment. (Simpson, C et al.)