Students will obtain knowledge about isolation and purification of biopolymers and other components present in various types of biological material. Further, stability of biomolecules, and methods of their characterization, analysis and applications will be demonstrated.

8. Isolation and purification of nucleic acids from different natural sources
a) isolation of DNA from calf thymus
b) isolation of RNA from yeasts
c) identification of DNA and RNA by reaction with diphenyl ammine

The course is focused on some methods of manipulation with biological material, basic biochemical separation as well as analytical techniques and basic processes using in enzymology. The knowledge obtained after passing the course can serve as the basis of following practical courses, especially practices on biotechnology and gene technologies.

Specification of controlled education, way of implementation and compensation for absences