Suche

Filtern

Bibliotheken

Enhanced characterization of the zebrafish brain as revealed by super-resolution track-density imaging

In this study, we explored the use of super-resolution track-density imaging (TDI) for neuroanatomical characterization of the adult zebrafish brain. We compared the quality of image contrast and resolution obtained with T 2 * magnetic resonance imaging (MRI), diffusion tensor-based imaging (DTI), T... Full description

In this study, we explored the use of super-resolution track-density imaging (TDI) for neuroanatomical characterization of the adult zebrafish brain. We compared the quality of image contrast and resolution obtained with T 2 * magnetic resonance imaging (MRI), diffusion tensor-based imaging (DTI), TDI, and histology. The anatomical structures visualized in 5 μm TDI maps corresponded with histology. Moreover, the super-resolution property and the local-directional information provided by directionally encoded color TDI facilitated delineation of a larger number of brain regions, commissures and small white matter tracks when compared to conventional MRI and DTI. In total, we were able to visualize 17 structures that were previously unidentifiable using MR microimaging, such as the four layers of the optic tectum. This study demonstrates the use of TDI for characterization of the adult zebrafish brain as a pivotal tool for future phenotypic examination of transgenic models of neurological diseases.

Enhanced characterization of the zebrafish brain as revealed by super-resolution track-density imaging

format:

Article

creator:

Ullmann, Jeremy

Calamante, Fernando

Collin, Shaun

Reutens, David

Kurniawan, Nyoman

subjects:

Zebrafish

Brain

Magnetic resonance

Diffusion-weighted imaging

Track-density imaging

Probabilistic tractography

ispartof:

Brain Structure and Function, 2015, Vol.220(1), pp.457-468

description:

In this study, we explored the use of super-resolution track-density imaging (TDI) for neuroanatomical characterization of the adult zebrafish brain. We compared the quality of image contrast and resolution obtained with T 2 * magnetic resonance imaging (MRI), diffusion tensor-based imaging (DTI), TDI, and histology. The anatomical structures visualized in 5 μm TDI maps corresponded with histology. Moreover, the super-resolution property and the local-directional information provided by directionally encoded color TDI facilitated delineation of a larger number of brain regions, commissures and small white matter tracks when compared to conventional MRI and DTI. In total, we were able to visualize 17 structures that were previously unidentifiable using MR microimaging, such as the four layers of the optic tectum. This study demonstrates the use of TDI for characterization of the adult zebrafish brain as a pivotal tool for future phenotypic examination of transgenic models of neurological diseases.

In this study, we explored the use of super-resolution track-density imaging (TDI) for neuroanatomical characterization of the adult zebrafish brain. We compared the quality of image contrast and resolution obtained with T 2 * magnetic resonance imaging (MRI), diffusion tensor-based imaging (DTI), TDI, and histology. The anatomical structures visualized in 5 μm TDI maps corresponded with histology. Moreover, the super-resolution property and the local-directional information provided by directionally encoded color TDI facilitated delineation of a larger number of brain regions, commissures and small white matter tracks when compared to conventional MRI and DTI. In total, we were able to visualize 17 structures that were previously unidentifiable using MR microimaging, such as the four layers of the optic tectum. This study demonstrates the use of TDI for characterization of the adult zebrafish brain as a pivotal tool for future phenotypic examination of transgenic models of neurological diseases.

Enhanced characterization of the zebrafish brain as revealed by super-resolution track-density imaging

description

In this study, we explored the use of super-resolution track-density imaging (TDI) for neuroanatomical characterization of the adult zebrafish brain. We compared the quality of image contrast and resolution obtained with T 2 * magnetic resonance imaging (MRI), diffusion tensor-based imaging (DTI), TDI, and histology. The anatomical structures visualized in 5 μm TDI maps corresponded with histology. Moreover, the super-resolution property and the local-directional information provided by directionally encoded color TDI facilitated delineation of a larger number of brain regions, commissures and small white matter tracks when compared to conventional MRI and DTI. In total, we were able to visualize 17 structures that were previously unidentifiable using MR microimaging, such as the four layers of the optic tectum. This study demonstrates the use of TDI for characterization of the adult zebrafish brain as a pivotal tool for future phenotypic examination of transgenic models of neurological diseases.

subject

0

Zebrafish

1

Brain

2

Magnetic resonance

3

Diffusion-weighted imaging

4

Track-density imaging

5

Probabilistic tractography

general

0

10.1007/s00429-013-0667-7

1

English

2

Springer Science & Business Media B.V.

3

SpringerLink

sourceid

springer_jour

recordid

springer_jour10.1007/s00429-013-0667-7

issn

0

1863-2661

1

18632661

2

1863-2653

3

18632653

rsrctype

article

creationdate

2015

addtitle

0

Brain Structure and Function

1

Brain Struct Funct

searchscope

springer_journals_complete

scope

springer_journals_complete

lsr30

VSR-Enriched:[pqid, galeid, pages]

sort

title

Enhanced characterization of the zebrafish brain as revealed by super-resolution track-density imaging

Enhanced characterization of the zebrafish brain as revealed by super-resolution track-density imaging

jtitle

Brain Structure and Function

stitle

Brain Struct Funct

risdate

201501

volume

220

issue

1

spage

457

epage

468

issn

1863-2653

eissn

1863-2661

genre

article

ristype

JOUR

abstract

In this study, we explored the use of super-resolution track-density imaging (TDI) for neuroanatomical characterization of the adult zebrafish brain. We compared the quality of image contrast and resolution obtained with T 2 * magnetic resonance imaging (MRI), diffusion tensor-based imaging (DTI), TDI, and histology. The anatomical structures visualized in 5 μm TDI maps corresponded with histology. Moreover, the super-resolution property and the local-directional information provided by directionally encoded color TDI facilitated delineation of a larger number of brain regions, commissures and small white matter tracks when compared to conventional MRI and DTI. In total, we were able to visualize 17 structures that were previously unidentifiable using MR microimaging, such as the four layers of the optic tectum. This study demonstrates the use of TDI for characterization of the adult zebrafish brain as a pivotal tool for future phenotypic examination of transgenic models of neurological diseases.