* The clay+AgNO<sub>3</sub> mixture was then transfered into the filter funnel with suction applied.

* The clay+AgNO<sub>3</sub> mixture was then transfered into the filter funnel with suction applied.

* Aluminum foil was used to cover the funnel to avoid silver to interact with light.

* Aluminum foil was used to cover the funnel to avoid silver to interact with light.

+

* After autoclave, the LB media were placed on counter without removing the aluminum foil. The Agar were cooled to about 50°C, until the wall of Erlenmeyer flask can be touched by hand. Approximately 20mL of the Agar were poured into each of the 20 separate petri dishes.

==Results==

==Results==

-

* The clay+AgNO<sub>3</sub> mixture when taken off the hot plate appeared black. However, during filtration, the filtered liquid solution remained clear in color.

+

* The clay+AgNO<sub>3</sub> mixture when taken off the hot plate appeared black. However, during filtration, the filtered liquid solution remained clear in color. However, the filtered liquid solution turned dark and obtained a darker red color after sitting on the counter. The filtered liquid solution was analyzed by Dr. Fox, who later concluded that silver nanoparticles were found in filtrate.

The Erlenmeyer flask obtaining LB media or Agar were covered with aluminum foil, the bottles containing LB were capped loosely. All media were autoclaved under liquid cycle.

The clay+AgNO3 mixture was taken off hot plate.

A filter funnel, attached to 500mL Erlenmeyer flask then attached to a pump, is covered with a paper filter. Surface of paper filter was wetted and attached onto filter funnel through adding water.

The clay+AgNO3 mixture was then transfered into the filter funnel with suction applied.

Aluminum foil was used to cover the funnel to avoid silver to interact with light.

After autoclave, the LB media were placed on counter without removing the aluminum foil. The Agar were cooled to about 50°C, until the wall of Erlenmeyer flask can be touched by hand. Approximately 20mL of the Agar were poured into each of the 20 separate petri dishes.

Results

The clay+AgNO3 mixture when taken off the hot plate appeared black. However, during filtration, the filtered liquid solution remained clear in color. However, the filtered liquid solution turned dark and obtained a darker red color after sitting on the counter. The filtered liquid solution was analyzed by Dr. Fox, who later concluded that silver nanoparticles were found in filtrate.