Description:
Human papillomavirus (HPV) is a diverse group of DNA-based viruses that infect skin and mucous membranes of humans and animals. Some HPV types are the causative agents of cervical cancer, with types 16 and 18 being particularly high-risk. The viral proteins E6 and E7 disrupt normal cell cycle regulation by interacting with p53 (a tumor-suppressing transcription factor) and Rb (retinoblastoma protein, also a tumor-supressor). E7 particularly binds to Rb and histone deacetylases, resulting in activation of the E2F genes, which code for a family of transcription factors. The viral proteins E6 and E7 might be of particular interest in the development of therapeutic vaccines, since they are expressed early in viral infection.

Applications: Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:Optimal dilutions to be determined by the researcher.

Recommended Pair:Suitable for use as the capture or solid phase antibody.Matched to P3105-16D, P3105-16F or P3105-25C as the detection or conjugate (label) antibody.

Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.