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Serena Williams, the French Open defending champion, is ousted in the second round; sister Venus booted, too

France Tennis French Open

Serena Williams of the U.S. leaves after losing her second round match of the French Open tennis tournament in two sets 2-6, 2-6, against Spain's Garbine Muguruza at the Roland Garros stadium, in Paris, France, Wednesday, May 28, 2014. (AP Photo/Darko Vojinovic)
(Darko Vojinovic)

Spain's Garbine Muguruza celebrates winning the second round match of the French Open tennis tournament defeating Serena Williams of the U.S. in two sets 6-2, 6-2, at the Roland Garros stadium, in Paris, France, Wednesday, May 28, 2014. (AP Photo/Darko Vojinovic)Darko Vojinovic

BIRMINGHAM, Alabama - World No. 1 and defending champion Serena Williams lost her second-round match at the French Open, falling 6-2, 6-2 to Spain's Garbine Muguruza.

Williams had previously won no fewer than five games in losing a Grand Slam match. The younger of the Williams sisters went down about an hour after older sister Venus lost 2-6, 6-3, 6-4 to Slovakia's Anna Schmiedlova.

"It's my biggest win so far," Muguruza told ESPN following the match. "Serena Williams, since I was a child I always admired her. It's a really big win."

Williams won the opening game of the match and then lost the next five as Muguruza took complete control, consistently hitting the ball hard down the middle.

"That was part of my plan, to be so aggressive," the Spaniard said. "Don't give her angles, don't make me run. I think I did really good."

In contrast, Williams was out of sorts most of the match. At one point, she could be heard saying, "I can't serve."

Muguruza won the final game at love.

"I was nervous," she admitted. "The last game, I (said), 'OK, do the same, concentrate. Don't lose your mind.' I think I handled really good my nerves."