Abstract: :
Purpose: The addition of indocyanine green (ICG) to mitomycin–C(MMC) may permit localization of antimetabolite during trabeculectomy.This would facilitate estimation of treatment area, recognitionof accidental application to inappropriate tissues, and spongelocalization. This study examined the effect of ICG on MMC–mediatedinhibition of Tenon's fibroblast proliferation.Methods: Fibroblast monolayers were exposed to either MMC (0.4mg/mlin PBS) or PBS containing indocyanine green (0.065%, 0.125%,0.250% and 0.5% in 200µl PBS) for 5 minutes. Controlswere exposed for 5 minutes to MMC, PBS or culture medium containingno ICG. Following treatment, the monolayers were washed andincubated in culture medium for 24, 48, 72hr and 1 week periods.At the conclusion of each incubation period, cell number wasassessed using the CyQUANT® cell proliferation assay.Results: The presence of ICG alone, at concentrations rangingfrom 0.0625% to 0.500%, had no effect on the rate of fibroblastproliferation. There was no significant difference in cell numberbetween controls and monolayers exposed to all concentrationsof ICG (range 32 to 34 x 10^2 (p <0.1)) up to one week postexposure. In contrast, MMC treatment resulted in a significantreduction in viable fibroblast number (8.4 ± 0.13 x 10^2)and loss of cell viability was observed. At all concentrationstested, ICG in combination with MMC did not alter fibroblastnumber up to 1 week compared to MMC alone (8.5 ± 0.05and 8.4 ± 0.12 x 10^2 respectively).Conclusions: ICG had no effect on fibroblast proliferation.ICG did not potentiate or diminish the effect of mitomycin Con Tenon's capsule fibroblast proliferation.