E-556

CYLD is a C67 type peptidase that has endo-deubiquitinase activity, particularly toward K63-linked polyubiquitin chains. CYLD is important in the regulation of pathways leading to NFkB activation, and thus plays a role in cell survival, proliferation and differentiation. Negative regulator of Wnt, TNFα, and TLR4 signaling by disassembly of K63-polyubiquitin chains utilized in these pathways. This recombinant protein contains an N-terminal 6-His tag.

Product Information

Stock:

X mg/ml (X µM) in 50 mM HEPES 8.0, 200 mM NaCl, 2 mM DTT

MW:

108 kDa

Purity:

>90% by SDS-PAGE

Use & Storage

Use:

Typical enzyme concentration to support hydrolysis of substrates is 50-500 nM depending on experimental conditions. A 15 minute preincubation with 10 mM DTT is recommended to achieve maximum activity.