Total RNA Purification from Just a Few Cells

With NucleoSpin RNA II, you can detect transcripts from very small numbers of cells—in this example, RNA was isolated from as few as 10 HeLa cells.

Total RNA was isolated from the indicated number of HeLa cells with NucleoSpin RNA II (elution volume: 40 µl). RT-PCR reactions were carried out with 3 µl of total RNA and beta-actin primers. The RT-PCR product was 626 bp.

Simple Protocol for DNA Removal

NucleoSpin RNA II uses a simple spin-column procedure that is probably already familiar to you. First, cells are lysed in a buffer that inactivates RNases (which are present in virtually any biological material) and creates appropriate conditions for RNA to bind to the silica membrane. After lysis, homogenization and reduction of viscosity are achieved by filtration with NucleoSpin Filter units (shredders), which are provided with the kit. Residual genomic DNA is removed by on-column digestion with rDNase, which is also included in the kit.