Use of cycloheximide

HI:
I use cycloheximide at the concentration of 5 ug/ml. I have tried different
concentrations and this one works best for me on B16 melanoma cells.
Interestingly, the gene of our interest is up-regulated by cycloheximide at
10-20 fold higher than control. When I add 10 ug/ml of medium, there is
decrease in expression. We have done protein synthesis analysis by labelled
amino acid. Protein synthesis is inhibited at 5 ug/ml of medium.
Good luck.
Dinakar