cj0414 and cj0415 mutants lack GADH activity at either temperature. Cj0415 mutant does not exhibit gluconate-dependent respiration. Cj0415 mutant is defective in chicken colonization, but exhibits only a minor colonization defect in mice

Campylobacter jejuni

Localization

Localization

Commentary

Organism

GeneOntology No.

Textmining

periplasm

-

Campylobacter jejuni

-

-

Molecular Weight [Da]

Molecular Weight [Da]

Molecular Weight Maximum [Da]

Commentary

Organism

26900

-

sequence analysis, cj0414

Campylobacter jejuni

63700

-

sequence analysis, cj0415

Campylobacter jejuni

Organism

Organism

Primary Accession No. (UniProt)

Commentary

Textmining

Campylobacter jejuni

-

strain 81-176

-

Campylobacter jejuni 81-176

-

strain 81-176

-

Substrates and Products (Substrate)

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

D-gluconate + NADP+

wild-type exhibits gluconate-dependent respiration. Genes cj0414 and cj0415, orthologous to GADH, are co-transcribed. Campylobacter jejuni 81–176 does not use gluconate compounds as sole carbon sources. GADH plays a minor role in mouse colonization

700265

Campylobacter jejuni

2-dehydro-D-gluconate + NADPH + H+

-

-

-

?

D-gluconate + NADP+

wild-type exhibits gluconate-dependent respiration. Genes cj0414 and cj0415, orthologous to GADH, are co-transcribed. Campylobacter jejuni 81–176 does not use gluconate compounds as sole carbon sources. GADH plays a minor role in mouse colonization

700265

Campylobacter jejuni 81-176

2-dehydro-D-gluconate + NADPH + H+

-

-

-

?

Cofactor

Cofactor

Commentary

Organism

Structure

FAD

-

Campylobacter jejuni

pI Value

Organism

Commentary

pI Value Maximum

pI Value

Campylobacter jejuni

cj0414, isoelectric focusing

-

5.5

Campylobacter jejuni

cj0415, isoelectric focusing

-

8.8

Activating Compound (protein specific)

Activating Compound

Commentary

Organism

Structure

additional information

expression and activity of cj0414 and cj0415 are higher at 42°C than at 37°C

Campylobacter jejuni

Application (protein specific)

Application

Commentary

Organism

medicine

ability of Campylobacter jejuni to use gluconate as an electron donor via GADH activity is an important metabolic characteristic that is required for full colonization of avian but not mammalian hosts

cj0414 and cj0415 mutants lack GADH activity at either temperature. Cj0415 mutant does not exhibit gluconate-dependent respiration. Cj0415 mutant is defective in chicken colonization, but exhibits only a minor colonization defect in mice

Campylobacter jejuni

Localization (protein specific)

Localization

Commentary

Organism

GeneOntology No.

Textmining

periplasm

-

Campylobacter jejuni

-

-

Molecular Weight [Da] (protein specific)

Molecular Weight [Da]

Molecular Weight Maximum [Da]

Commentary

Organism

26900

-

sequence analysis, cj0414

Campylobacter jejuni

63700

-

sequence analysis, cj0415

Campylobacter jejuni

Substrates and Products (Substrate) (protein specific)

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

D-gluconate + NADP+

wild-type exhibits gluconate-dependent respiration. Genes cj0414 and cj0415, orthologous to GADH, are co-transcribed. Campylobacter jejuni 81–176 does not use gluconate compounds as sole carbon sources. GADH plays a minor role in mouse colonization

700265

Campylobacter jejuni

2-dehydro-D-gluconate + NADPH + H+

-

-

-

?

D-gluconate + NADP+

wild-type exhibits gluconate-dependent respiration. Genes cj0414 and cj0415, orthologous to GADH, are co-transcribed. Campylobacter jejuni 81–176 does not use gluconate compounds as sole carbon sources. GADH plays a minor role in mouse colonization