C. jejuni Full ORF Expression Clones

C. jejuni Full ORF Expression Clones

Campylobacter jejuni is the most commonly recognised cause of bacterial gastroenteritis in humans. Initial difficulties in culture requirements, and attempts at genetic modification held back the progress in understanding this organism compared to other enteric pathogens.

The sequencing of 1.64 million nucleotides in the C. jejuni genome at the Wellcome Trust Sanger Institute has heralded a new era in C. jejuni research. Using this sequence information, along with 31 predicted ORF's by TIGR, Jodi Parrish (Russell Finley's lab - Wayne State University School of Medicine) has created a C. jejuni ORF library.

Campylobacter jejuni is the most commonly recognised cause of bacterial gastroenteritis in humans. Initial difficulties in culture requirements, and attempts at genetic modification held back the progress in understanding this organism compared to other enteric pathogens.

The C. jejuni ORF library is a proteome-scale expression clone set comprising of more than 1,600 ORF's. The clones were generated by efficiently utilizing the native E. coli recombination system.

Recombination sites have been confirmed by sequencing, and most of the clones have been 5' sequence verified. The ORFs range from 45 - 4020bp in length. After expression, affinity purification can be performed on the proteins using either GST or His-tags.

The clone set has many applications, including protein interactions, activity, and structural studies. Furthermore, the clones can be utilised for gene expression patterns by micro-array analysis. The process of endogenous recombination in E. coli facilitates the insertion of the ORF in the correct 5' to 3' direction. The antibiotic resistance gene, encoded on the plasmid, allows for the selection of the new construct.

The library has many applications including but not limited to: Drug targets Over expression analysis Protein production

Please click on Cjejuni.xls to view the clone database.Due to MTA requirements, individual clones should be ordered online using the Cj clone ID (e.g. Cj0748) in the search box below:

The complete set can be ordered below:

Product Code

Name

Price

5181_EX-Cjejuni_ORF

C.jejuni Full ORF Expression Clones

£591.00

This is the diagrammatic representation of the expression and cloning regions of pTLJ03 ( vector map, sequence). The process of endogenous recombination in E. coli facilitates the insertion of the ORF in the correct 5' to 3' direction. The antibiotic resistance gene, encoded on the plasmid, allows for the selection of the new construct.