Cell proliferation assays provide a critical piece of the puzzle when evaluating cell health, genotoxicity, and the efficacy of anti-cancer drugs. When compared with antibody-based BrdU methods, the Click-iT® Plus EdU cell proliferation assays not only offer better performance and an easier workflow but are now compatible with an even broader range of fluorescent probes—including GFP, RFP, and phycobiliproteins—allowing you to perform multiplex assays that provide a more informative picture of the state of the cell.

EVOS® Light Cube and fluorophore selection guide poster

EVOS® Light Cubes integrate LEDs and high-performance filters in a novel illumination system that delivers precise control, minimal maintenance, and exceptional reliability. These LED cubes are compatible with the EVOS® FL and FL Auto Imaging Systems, as well as the Countess® II FL Automated Cell Counter. Use the selection guide on this poster to choose the light cube that best matches your dye.

Journal Club

Online and on the move

The Molecular Probes® Cell Imaging Reagent Guide and Protocols mobile app is designed to help you find fluorescent reagents, kits, and protocols for cell biology–related fluorescence microscopy applications. Also included are product guides for instrument calibration and setup, protein labeling, and secondary detection, as well as examples of data output. Two key features include: (1) a built-in protocol timer that runs even if you leave the app and (2) excitation and emission data for every product.

The Molecular Probes® flow cytometry app

The Molecular Probes® Flow Cytometry Reagent Guide and Protocols mobile app is designed to help you find fluorescent reagents, kits, and protocols for cell biology–related flow cytometry applications. Features include product guides for instrument calibration and setup. detailed examples of data output, and excitation and emission data for every product.

Expert opinion: The basics of multicolor flow cytometry panel design

With the proliferation of new fluorescent dyes, as well as instruments that can detect 18 or more parameters, multicolor flow cytometry has become more popular and more accessible than ever. When designing polychromatic flow cytometry panels, there are dozens of factors to consider. As the complexity of the experiment increases, so does the number of parameters that need to be analyzed and the technical issues that need to be addressed.

To help sort through the challenges of panel design, we reached out to an expert in flow cytometry: Holden Maecker, PhD. Dr. Maecker is the Director of the Human Immune Monitoring Center and also an Associate Professor of Microbiology and Immunology at Stanford University. In the “Basics of Multicolor Flow Cytometry Panel Design” webinar, Dr. Maecker covers the key rules for choosing fluorescent dye combinations and filter sets and illustrates these rules with plenty of examples and practical applications. He also discusses controls and standardization, which are absolutely critical in ensuring quality data from multicolor flow panels, as well as the relevance of panel design to new mass cytometry platforms.