Detection and Quantification of IP Enrichment

Was PCR analysis performed on a serial dilution of the 2% input DNA for the primer set used (described in Section G, Step 1)?

Was the PCR of the input DNA serial dilution linear using this primer set?

What was the calculated PCR efficiency for the primer set used (for quantitative PCR users only)?

Did the control Histone H3 Antibody (#4620) show enrichment of the control RPL30 promoter? If yes, what was the amount of enrichment, as a percent of the total input chromatin added to the IP reaction?

What was the amount of enrichment of the RPL30 promoter using Normal Rabbit IgG (#2729), as a percent of the total input chromatin?

Did the control Histone H3 Antibody (#4620) show enrichment of the target locus? If yes, what was the amount of enrichment, as a percent of the total input chromatin?

What was the amount of enrichment of the target locus using Normal Rabbit IgG (#2729), as a percent of the total input chromatin?

Did the test antibody show enrichment of the target locus. If yes, what was the amount of enrichment as a percent of the total input chromatin?