Images, Video, and Other Other Media

Merged bright field and fluorescence images of 28 serial optical sections generated
by
confocal laser scanning microscopy (CLSM). EC1-GFP (green signal) is secreted by the
egg cell (ec) when the two sperm cells (sc1 & sc2; red fluorescent nuclei) arrive
at the
site of gamete interactions. Each frame of this scan represents one optical section
of 0.48
μm, the z-stack size is 13.44 µm. Two individual optical sections of this scan are
shown
in Fig. 2F and in the inset of Fig. 2F. The scan was performed 7.5 hours after handpollinating
pistils transgenic for EC1.1p:EC1-GFP with the marker line HTR10-mRFP1.
The positions of the central cell (cc), the egg cell (ec) and the degenerating synergid
(dsyn) are indicated. The same scan is shown in movie S2, as overlay of red and green
signals only.

Overlay of red and green fluorescent images of the same CLSM scan shown in movie S1.
Secretion of EC1-GFP (green signal) can be observed when the two sperm cells (sc1
&
sc2; red fluorescent nuclei) arrive at the site of gamete interactions. Each frame
of this
scan represents one optical section of 0.48 µm, the z-stack size is 13.44 μm. Two
individual optical sections of this scan are shown in Fig. 2G and in the inset of
Fig. 2G.
The scan was performed 7.5 hours after hand-pollinating pistils transgenic for
EC1.1p:EC1-GFP with the marker line HTR10-mRFP1. The positions of the central cell
(cc), the egg cell (ec) and the degenerating synergid (dsyn) are indicated. The same
scan
is shown in movie S1 as merged bright field and fluorescence images.