Figure 4.

ZsGreen-Vpr localizes exclusively in the cytoplasm within Jurkat lymphocytic cells. While control ZsGreen protein (A) was evenly distributed, both ZsGreen-Vpr (B) and
Vpr-ZsGreen (C) were found in speckles only within the cytoplasmic compartment, completely
excluded from the nucleus. First, second, and third columns are DAPI, FITC, and merged
views, respectively. Each figure represents a cross-sectional view of the cell and
is representative of the entire cell population observed.