Cloning Vectors—In-Fusion Ready Fluorescent Protein Vectors

To simplify the construction and testing of fluorescent protein fusions, we provide prelinearized versions of our popular DsRed-Monomer-N1/C1 and AcGFP1-N1/C1 cloning vectors for fast, efficient PCR cloning with In-Fusion. Simply use appropriate gene-specific PCR primers encoding 15 nucleotide 5' extensions homologous to the ends of these prelinearized vectors to amplify your gene of interest. A single insert can be cloned directly into any one of these four In-Fusion Ready vectors; saving time and allowing parallel cloning of fusion proteins with different colors at either the N- or C-terminus. This is important when the ability of a protein of interest to function either as an N- or C-terminal fusion is unknown.

Features

Flexible cloning—use one PCR product, with choices of DsRed-Monomer-N1/C1 or AcGFP-N1/C1

Easily test protein functionality as N- or C-terminal fusions

Prelinearized for simple, one-step In-Fusion cloning

Applications

In-Fusion cloning

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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