P231 The Identification of Epidermal Fatty Acid Binding Protein in Advanced Head and Neck Cancer Using a Proteomic Approach

Extract

Objective: To identify proteins overexpressed in metastatic head and neck cancers using a proteomic approach.

Design: We identified differential protein expression in 10 head and neck tumors with lymph node metastasis and 3 head and neck tumors without metastasis using 2-dimensional gel separation techniques and Western blot analysis.

Results: A protein of interest, which was present in 12 (92%) of the 13 tumors, demonstrated significantly greater expression in metastatic primary tumors. The protein spot was identified as epidermal fatty acid binding protein (E-FABP) using matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) and sequenced with an ion trap mass spectrometer. Subsequent Western blot analysis of head and neck tumors using an anti–E-FABP antibody demonstrated heavy expression in 3 of 3 patients with N2 or greater neck disease and lower levels of expression in tumors with N1 (n = 1) or N0 disease (n = 2). Normal control oral mucosa demonstrated light (n = 2) or no staining (n = 3). Due to its low molecular weight, E-FABP is filtered by the kidney. Western blot analysis of patient urine detected E-FABP in the urine of 5 (83%) of 6 patients with head and neck cancer.

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