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Equipment

We use real-time PCR to amplify and simultaneously quantify a targeted DNA/RNA molecule. It enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of one or more specific nucleotide sequences in a sample. The procedure follows the general principle of polymerase chain reaction; its key feature is that the amplified DNA is detected as the reaction progresses in real-time.

Provides an absolute quantitation of target DNA molecules with unrivaled accuracy, precision, and sensitivity. ddPCR applications include measurement of copy number variation, rare sequence detection, mutation detection, and gene expression analysis. More information on the instrument can be found here.

Provides an absolute quantitation of target DNA molecules with unrivaled accuracy, precision, and sensitivity. ddPCR applications include measurement of copy number variation, rare sequence detection, mutation detection, and gene expression analysis. More information on the instrument can be found here.