Application Information: Heme Oxygenase 1 antibody

IHC: Use at an assay dependent dilution. WB: 1:2000. Detects a band of approximately 32 kDa (predicted molecular weight: 34.6 kDa). Optimal dilutions/concentrations should be determined by the end user.

Positive Controls

Recombinant Human and Rat HO-1 (Hsp32) Protein

Form Supplied

Liquid

Concentration

1
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Protein A affinity purified

Purification Note

From polyclonal serum

Specifications: Heme Oxygenase 1 antibody

Full Name

heme oxygenase (decycling) 1

Background

Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO1 and a constitutively expressed HO2. HO1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.