Abstract:
...in the August issue of the journal, Johns Hopkins scientists have devised an "ultrasensitive and reliable nanotechnology assay, MS-qFRET, for detection and quantification of DNA methylation." Their method uses bisulfite DNA, PCR, and quantum dots that can determine the methylation status from FRET. Key features, they say, are low background noise, high resolution, and high sensitivity; it can also be used on clinical samples for early diagnosis, prognostic application, and monitoring of therapeutics.