The Procedure :

Procedure continued:

8 Procedure continued Sanitize tissue sample Place tissue sample on media in test tube Cap or seal test tube Place in warm, well-lighted area Plantlets should begin to form in 4-6 weeks

Tissue Culture Process:

9 Tissue Culture Process Start Tissue Removed Media Callus Plantlet

Advantages Disadvantages:

10 Advantages Disadvantages Small part of plant is required Produce many plantlets in small areas Disease-free plants Pest-resistant Maintaining a sterile environment Expensive lab facilities Poor photosynthesis Water loss

Guided Practice:

11 Guided Practice Group Activity: Put the steps for tissue culture in order with your group.

Independent Practice:

12 Independent Practice What is another name for tissue culture? Define tissue culture in your own words. List one advantage and one disadvantage of tissue culture. What four materials are required for the tissue culture process?