can someone tell me how to extract plant proteins n how to purify them?

thanks a million

-S10016208-

hi

there are lots of methods

i personally do a very simple one

weigh the plant leaves use liquid nitrogen / freeze them in - 70 for a couple of hours

grind well using ice cold 1X PBS ( u can use 2mM PMSF or other inhibitors of proteinases )transfer to eppendorff or centrifuge tubescentrifuge at 10000 rpm for 10 min at 4 degreecollect the supernatant

this will be total protein

after this u can do acetone purificationadd ice - cold acetone , 2- 3 times the volume of supernatant keep at - 70 for 1- 130 hourcentrifuge again same conditions as previous centrifuge stepsave pelletwash the pellet with 70% cold ethanoldry the pelletdissolve in 0.1M Tris Hcl ( ph 8.3)