jstrassw at opal.tufts.edu wrote:
>> Hello!
> I am trying to use 293 cells because they are reported to be highly
> transfectable (>50% by Calcium Phosphate) and they are EGF-responsive. The
> only problem is that the buggers dont adhere well to dishes. I am loosing a
> lot of cells every time I wash after transfection. Any suggestions?
>> Thank you,
> John
John: You are right, 293 cells don't adhere firmly to culture dishes. To
avoid losing cells, try to pipet fluids (medium or PBS) CAREFULLY /
SLOWLY
into the edge of your culture dish. If you pipet fluids forcefully onto
your 293 cells they will get washed away, especially if cells are
confluent.
(BTW, I had the same problem when I started working with these cells.)
Good luck!
Wolfram