PR3 (c-ANCA) ELISA kit description:

Diagnostic Automation Proteinase-3 (PR-3) ELISA is intended for the detection and semi-quantitative determination of antibodies to PR-3 in human sera. The assay is to be used to detect antibodies in a single serum specimen.

PR3 (c-ANCA) ELISA Kit Background Information:
Proteinase-3 (PR3)is a lysosomal enzyme that can be found in human neutrophils. There are two subsets of autoantibodies to human neutrophils: c- ANCA and p-ANCA. The subtype c-ANCA, shows cytoplasm staining by IFA and is diagnostic for Wegener granulomatosis. The major antigen responsible for the c-ANCA staining is PR3. PR3 is the most common antigen target of ANCA in patients with granulomatosis with polyangiitis where c-ANCA is found over 90% of the time. The second subtype p-ANCA, shows perinuclear staining by IFA. The major antigen responsible for the p-ANCA response has been shown to be myeloperoxidase. The ANCA staining patterns are obtained using ethanol-fixed human neutrophils. Anti- PR-3 autoantibodies have been associated with Wegener granulomatosis but less often with microscopic polyangiitis.

PR3 (c-ANCA) ELISA Test Principle:
PR-3 ELISA test is an Enzyme-Linked Immunosorbent Assay to detect IgG, IgA, and IgM antibodies to PR-3 antigens. Purified PR-3 antigens are attached microplet wells. Diluted test sera are added to each well. If the antibodies are present that recognize the antigen, antigen-antibody complexes are formed. After incubation, the wells are washed and an enzyme labeled anti-human IgG, A, M, is added to each well. If antibody is present the conjugate will bind to the antigen-antibody complexes. For additional details please refer to the instructions for use.

Product inserts for all Autoimmune ELISA kits follow a similar method. See the PR3 (c-ANCA) ELISA Kit product insert for specific details on preparation, procedures, quality control, and test result interpretation.