Minimally charged, for less non-specific binding than Alexa Fluor® 488
Narrower emission spectrum for less bleed to red
Very photostable
Compatible with super-resolution imaging by TIRF
Highly water soluble and pH-insensitive

Yields the brightest antibody conjugates among spectrally
similar dyes.
Excellent choice for multicolor imaging with green dyes like CF®488A
Extremely photostable
Also see CF®594ST, a version of CF®594 engineered specifically for STORM microcopy

CF®430 and CF®440 are violet excitable dyes with green fluorescence emission, compatible with the AmCyan channel for flow cytometry. CF®430 and CF®440 are much more photostable than spectrally similar dyes, making them suitable for imaging applications using confocal microscopy with 405 nm excitation. CF®430 is a perfect match for the cyan fluorescent protein (CFP) filter set. See CF®430 and CF®440 product lines below. Additional conjugates or reactive dyes may be added upon request; contact techsupport@biotium.com to inquire.

CF®430 Technical Summary

Abs/Em maxima: 426/498 nm

Extinction coefficient: 40,000

Molecular weight: ~429

Excitation laser line: 405 nm

Replaces: Pacific Green™, BD Horizon™ V500, Krome Orange™

CF®440 Technical Summary

Abs/Em maxima: 440/515 nm

Extinction coefficient: 40,000

Molecular weight: ~706

Excitation laser line: 405 nm

Replaces: Alexa Fluor® 430

Figure 2. CF®430 and CF®440 are much more photostable than spectrally similar dyes. Cells were stained with biotinylated antibodies followed by streptavdin conjugates of the indicated dyes. Fluorescence was imaged on a Zeiss LSM700 confocal microscope in the FITC channel using 405 nm excitation. Images were acquired every 5 seconds for 12 consecutive scans. Mean fluorescence intensity was normalized to time 0.

CF is a registered trademark of Biotium, Inc. Alexa Fluor is a registered trademark of Thermo Fisher Scientific; Horizon is a trademark of BD Biosciences; Krome Orange is a tradmark of Beckman Coulter.

CF® initially was an abbreviation for Cyanine-based Fluorescent dyes. These were the first patented CF® dyes based on cyanine dye structures. Since then, our CF® dye patent portfolio has expanded to include four different fluorescent dye core structures that cover the fluorescence spectrum from UV to NIR.

The exact chemical structures of CF® dyes are currently confidential but will be fully disclosed at a later stage when pending patents become granted. In general terms, the structure of a CF® dye may be divided into two parts: a) dye core structure (i.e. the aromatic ring skeleton that defines the dye’s color or absorption/emission wavelengths), and b) core structure-modifying elements. At present, CF® dyes bear the core structures of coumarin, pyrene, rhodamine or cyanine dyes. Blue fluorescent CF® dyes are based on coumarin or pyrene dye core structure, while green to near-IR CF® dyes are based on either cyanine or rhodamine dye core structures. Core structure-modifying elements refer to various chemical attachments to the core structure and are a key aspect of the CF® dye invention that makes CF® dyes superior to other commercial dyes.