Glee's Jane Lynch, Chris Colfer, Lea Michele, Cory Monteith and Amber Riley were seen enlisting the stars to perform the song, but rejected Gosselin, who was sporting the same costume she wore during her stint on Dancing With The Stars.

Fallon then appeared on stage with an acoustic guitar to finish off the track.

"NBC asked me, the host of Late Night, to come to Los Angeles. What could possibly go wrong?" he asked, before the camera panned to Conan O'Brien, who departed NBC in controversial circumstances earlier this year after presenting The Tonight Show.