Application Information: RANGAP1 antibody, N-term

Approx 70kDa band observed in Human Brain and 3T3 lysates (predicted MW of 68kDa according to NP_002874). Recommended for use at 0.1-0.5µg/ml. In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatocytes. Recommended concentration, 2-4µg/ml. Please note that in mouse, there is a hypothetical protein called "similar to RANGAP1" (XP_139737.2) that is virtually identical.

Positive Controls

The peptide used to product this antibody is available for purchase (Catalog Number:GTX22081-PEP).

Form Supplied

Liquid

Concentration

0.5
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Specifications: RANGAP1 antibody, N-term

Full Name

Ran GTPase activating protein 1

Background

Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP 1 and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs. RanGDP involved in signaling. Mammalian RanGAP 1 is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358.