Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporins". Over the last decade, genes for several members of aquaporin family (AQP0, AQP1-15) have been cloned, expressed, and their distribution studied in many tissues. The individual members of aquaporin family have identical predicted secondary structures with up to 6 highly conserved hydrophobic membrane spanning domains (about 18-25aa each) and two conserved NPA motifs. However, N/C-terminal regions of AQPs are only ~20% conserved.

Catalog #

A2990

AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 26aa; gene locus 7p14) has been the foundation of the growing family of aquaporin. The lens specific AQP0 represents ~80% of total lens membrane protein.

Applications

Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilutions

Western Blot: 1-10ug/ml (ECL)

ELISA: 1:100,000; Use 50-100ng control peptide per well

Optimal dilutions to be determined by researcher

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.