... cappedRNA having a modified cap nucleotide and for use of such modified-nucleotide-cappedRNA molecules. In particular, ... cappedRNA having a modified cap nucleotide and for use of such modified-nucleotide-cappedRNA molecules. In particular, the ... present invention provides kits and methods for cappingRNA using a modified cap nucleotide and a capping enzyme system, such ...

New RNAcap analogs are disclosed containing one or more phosphorothioates groups. The analogs also contain modifications at ... "capped." In addition, there are some other forms of RNA that are also capped, for instance small nuclear RNAs (snRNAs). The cap ... 5′ Degradation of mRNAs Capped with Phosphorothioate Cap Analogs. Because short RNAscapped with m27,2′-OGppspG (D2) were ... A. Pasquinelli et al., "Reverse 5′ caps in RNAs made in vitro by phage RNA polymerases," RNA, vol. 1, pp. 957-967 (1995), ...

... cap-snatching' from host pre-messenger RNA, to transcribe its RNA genome into mRNA and a stuttering mechanism to generate the ... Comparing the FluA and FluB structures suggests that cap-snatching involves in situ rotation of the PB2 cap-binding domain to ... Here we use crystal structures of bat influenza A and human influenza B polymerases (FluA and FluB), bound to the viral RNA ... direct the capped primer first towards the endonuclease and then into the polymerase active site. The polymerase probably ...

A Protective Cap for Bacterial RNA. For the first time, researchers from Heidelberg University have deciphered the function of ... These molecular helpers remove the protective cap at the start of ribonucleic acid (RNA) molecules. This decapping destabilises ... Dr Andres Jäschke and his bioorganic chemistry working group now revealed these mechanisms in bacterial RNA. (Mehr in: ... August 3rd, 2016 , Tags: cell, CT, researcher, RNA , Category: Medizin-Biologie-Physik ...

In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is ... ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding ... RNAcap binding Source: UniProtKB ,p>Traceable Author Statement,/p> ,p>Used for information from review articles where the ... ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding ...

RNACaps/metabolism. *Reverse Transcriptase Polymerase Chain Reaction. *Sequence Homology, Amino Acid ... Bottom Line: In this study, we describe a novel Val/Arg/Gly (VRG) site in the PB2 cap-binding domain, which is involved in ... Bottom Line: In this study, we describe a novel Val/Arg/Gly (VRG) site in the PB2 cap-binding domain, which is involved in ... A novel functional site in the PB2 subunit of influenza A virus essential for acetyl-CoA interaction, RNA polymerase activity, ...

The CoV genome encodes several RNA processing enzymes involved in RNAcapping. The cap formation step by CoV has mainly been ... O-MTase to form cap-1 and cap-2 structures, respectively (Furuichi and Shatkin, 2000). Ribose 2′-O-methylation of viral RNAcap ... Apparently, the enzyme is not specific for viral substrate RNAs (Bouvet et al., 2010). Conversion of cap-0 to cap-1 structures ... cap) at the N7 position to produce a cap-0 structure (m7GpppN). Higher eukaryotes and viruses usually further methylate the cap ...

In the CBC complex, ncbp2/cbp20 recognizes and binds cappedRNAs (m7GpppG-cappedRNA) but requires ncbp1/cbp80 to stabilize the ... The conventional cap-binding complex with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in ... movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. ... RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the ...

RNACAPPING. ELONGATION. SPLICING. CLEAVAGE. POLYADENYLATION AND TERMINATION. EXPORT FROM THE NUCLEUS TO THE CYTOSOL. ... RNA comes in several varieties; the one of interest to us here is known as messenger RNA, or mRNA for short. Its job is to read ... DNA enlists the help of a closely related molecule known as RNA (for ribonucleic acid). RNA is also made from four bases, A, G ... The answer lies with another set of RNA molecules, called transfer RNA, or tRNA for short. Each particular tRNA molecule brings ...