Statement of Series 2 experimental question & goals. Outline of experimental process including : Mutant Hunt, Linkage Analysis, Mapping the Mutation to a chromosome and to a particular location, Complementation Analysis, Determining whether or not the mutation has been previously characterized, & Defining the gene sequence and protein change through DNA sequence analysis. 1-2 page summary of how you will determine the exact location of the gene and the sequence change likely to be associated with a phenotypic anomally BISC_219/F10: Assignment_Series2_Outline_Summary; Read the journal article,, and be prepared to discuss in LAB 3 the questions found at :BISC_219:Questions to Guide Your Reading 1.

Diagram the expected results (genotype and phenotype)for the crosses and self-fertilization progeny in the Linkage Testing work that you will do over the next several weeks. Highlight the progeny that will be used to continue each step of the process. Include a narrative description of the rational for the crosses and summarize the interpretation of the results that will allow determination of the linkage group to which your mutated gene of interest belongs. Assignment information and rubric found at BISC_219/F10: Assignment_Series2_Linkage Testing Crosses

5

Lab 4

Lab 5

Diagram the expected results (genotype and phenotype)for the crosses and self-fertilization progeny in the Mapping work that you will do over the next several weeks. Make sure you highlight the progeny that will be used to continue each step of the process. Assignment information and rubric found at: BISC_219/F10: Assignment_Series2_Mapping Crosses

Analyze your mutant gene's DNA sequence and write a 1-2 page summary describing briefly and generally how chain-termination sequencing is accomplished and then specifically describe the mutation most likely to be responsible for your phenotype change by comparing the wild type sequence to the mutant's gene. Include the resulting protein or regulatory product change and its likely significance in the worm and, if possible, in other species. Assignment information and rubric found at: BISC_219/F10: Assignment_Series2_DNA Sequencing

Construct figures/tables with properly formatted legends to illustrate the main findings of the RNAi part of your Series3:Reverse Genetics project. These figures are a draft of those that will be part of the results section of the research report you will write for Series3.