MyTaq™ DNA Polymerases

MyTaq™ Polymerases & Mixes

The MyTaq™ product range is a new generation of very high performance PCR products, designed to deliver outstanding results on all templates, including complex genomic DNA templates.

MyTaq is based on the latest technology in PCR enzyme preparation, engineered to increase affinity for DNA, resulting in significant improvements to yield, sensitivity and speed. The enzyme is supplied with an industry-leading novel buffer system, containing dNTPs, MgCl2 and enhancers at optimal concentrations, removing the need for optimization and giving superior amplification, making it the perfect choice for complex templates. MyTaq Mix is available as a ready-to-use 2x mix for setting up a trouble-free PCR reaction and there is the choice of an inert red dye, used for direct gel loading.

““With MyTaq I was able to dramatically boost my efficiencies and increase reproducibility. In addition, I found that multiple primer pairs produced cleaner products with improved melting curves.”

UC Davis School of Veterinary Medicine, USA

MyTaq™ HS Polymerases & Mixes

MyTaq™ HS is a new generation of very high performance, antibody-mediated hot-start PCR products, designed to give outstanding results with complex genomic DNA templates.

MyTaq HS uses the latest technology in PCR enzyme design, engineered to increase affinity for DNA, so resulting in significant improvements in yield, sensitivity and speed. The product also has the added convenience of room temperature reaction assembly, without non-specific amplification and primer-dimer formation. The enzyme is supplied with an industry-leading novel buffer system, containing dNTPs, MgCl2 and enhancers at optimal concentrations, removing the need for optimization and giving superior amplification, making it the perfect choice for complex templates. MyTaq HS Mix is available as a ready-to-use 2x mix for setting up a trouble-free PCR reaction and there is the choice of an inert red dye, used for direct gel loading.

Products

MyTaq™ One-Step RT-PCR Kits

The MyTaq™ One-Step RT-PCR Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent PCR in a single tube. A combination of the latest advances in buffer chemistry together with a reverse transcriptase and hot-start DNA polymerase system ensures that MyTaq One-Step RT-PCR Kit produces fast, highly-specific and ultra-sensitive one-step RT-PCR, perfect for all downstream applications. MyTaq One-Step Kit consists of reverse transcriptase, 2x MyTaq HS Mix and a potent RNase Inhibitor, RiboSafe, that are added together to create a simple to use all-in-one mix.

Products

“When we compared the performance of our routine supplier’s RTase against Bioline’s MyTaq One-Step RT-PCR, the other supplier’s RTase gave two false negatives in five different grapevine samples tested for Grapevine rupestris stem-pitting-associated Foveavirus. We were convinced to immediately switch.”

University of Adelaide, Australia

MyTaq™ Direct PCR Kits

Bioline direct-PCR kits deliver unparalleled convenience for DNA amplification by allowing PCR directly from crude samples. Based on MyTaq™ HS DNA Polymerase the kits have been specifically engineered to overcome PCR inhibitors typically present in crude samples and allow fast cycling conditions to be used, without compromizing PCR specificity and yield. High sensitivity and specificity also make direct-PCR kits highly-suited to multiplex PCR and high-throughput genotyping assays from small amounts of source material, generating significant savings in both time and cost.

Products

MyTaq Plant-PCR Kit Workflow

MyTaq Blood-PCR Kit Customer Review

“I’ve tested a number of products and by far the best one was MyTaq Blood-PCR Kit. The major advantage is that it works on blood samples and crude lysates. This product will also amplify larger amplicons than other products tested, even in a multiplex scenario. Other key advantages include the hot-start and its ability to withstand 30 freeze-thaw cycles.”

Markus Zeller, AutoGenomics, California, US

Reviews

"MyTaq Extract-PCR kit was effective and efficient for high throughput ..."