α7 nicotinic acetylcholine receptors (nAChRs) are implicated in multiple brain disorders such as Alzheimer’s disease, schizophrenia, addiction and ADHD. We have previously shown that α7 nicotinic acetylcholine receptors (nAChRs) regulate the excitation of pyramidal neurons in the prelimbic region of the mouse medial prefrontal cortex (mPFC) (1). The mPFC receives afferent glutamatergic input from numerous brain regions, including the ventral hippocampus, thalamus and amygdala. The aim of this study was to elucidate which afferent inputs express functional α7nAChRs.