Figure 3.

NFI binding correlates with histone H3 methylations: H3K4me3, H3K36me3, H3K9me3. 2881 NFI occupied genes in mouse embryonic fibroblasts and 3 groups of randomly
selected genes were defined as in Figure 2. ChIP-Seq data for 4 different histone
H3 methylations were obtained from the same cell type (mouse embryonic fibroblasts)
[4]. Average ChIP-Seq tag counts were calculated in windows of 50 bp for a region of
5 kb up- and down-stream of the orientated transcription start sites (TSS). Tag counts
were normalized globally, as a fold increase over the genome average tag count in
a window of 50 bp for the following modifications: A. H3K4me3, B. H3K36me3, C. H3K27me3,
and D. H3K9me3. Obtained data points were connected to form a continuous line. Arrows
indicate the orientation of transcription in each panel.