The large diversity of cells that comprise the human immune system requires methods that can resolve the contributions of individual cells to an immunological response. The release of cytokines is one of several important ...

Relatively little is known about metastatic cancer. The vast majority of cancer genome profiling (~99%) is done on primary tumors; yet, metastatic cancer is attributed to >90% of cancer-related deaths. The underlying ...

Type II Diabetes mellitus is a genetically complex disease characterized by insulin resistance in peripheral tissues, which results in simultaneous hyperglycemia and hyperinsulinemia. Because of the prevalence of type II ...

(cont.) on a set composed of the Cartesian product between the parameter bounds and the state bounds. Furthermore, I show that the solution of the differential equations is affine in the parameters. Because the feasible ...

The goals of this thesis are the development of global optimization algorithms for semi-infinite and generalized semi-infinite programs and the application of these algorithms to kinetic model reduction. The outstanding ...

Optimization is a key activity in any engineering discipline. Global optimization methods, in particular, strive to solve nonconvex problems, which often arise in chemical engineering, and deterministic algorithms such as ...

Systems that exhibit both discrete state and continuous state dynamics are called hybrid systems. In most nontrivial cases, these two aspects of system behavior interact to such a significant extent that they cannot be ...

(cont.) The effects of recombinant protein production were observed through expression analysis of induced, uninduced, and Empty-Vector cultures. As expected, recombinant α₁AT production led to increased expression of ...

A major hurdle to evolutionary engineering approaches for multigenic phenotypes is the ability to simultaneously modify multiple genes rapidly and selectively. Here, we describe a method for in vivo targeted mutagenesis ...