Cloning

Our range of products for DNA cloning includes transfection reagents, the GST Gene Fusion System comprised of GST pGEX Vectors and sequencing primers, as well as nucleotides and natural DNAs. Enzymes and kits are available for DNA modification by simple cloning using blunt ends or 5’ overhangs. The Recombinant Phage Antibody System (RPAS) is designed to clone mouse antibody genes and to express and detect functional antibodies.

Molecular cloning involves inserting an isolated DNA molecule into a suitable vector and then transferring the vector to a host cell for propagation. Cloning can be used to generate large numbers of copies of DNA for sequencing. Alternatively, coding sequences can be cloned into expression vectors specifically designed to produce large amounts of recombinant protein for purification or functional analysis. Polymerase Chain Reaction (PCR) and the combination of PCR with reverse transcription have revolutionized DNA cloning, enabling reverse-engineering of expressed genes. Cloning was one of the first techniques to demand extensive enzymatic modification of DNA. Modification steps include cleavage at specific positions, modification of fragment ends, addition of groups such as labels, and linking fragments together.