698μM iodophenol solution was made in 1mL DMSO, since it's not soluble in water. 2.5mM 4-aminoantipyrine (AAP) solution was prepared with the sodium phosphate buffer.

From the stocks, solution was prepared with 18mM iodophenol, 7.285μM AAP, 1.7mM H2O2 and 115nM HRP.

Such a solution was analyzed for the 1st, 3rd and 4th runs.

For the 2nd run, HRP was increased to 2.3μM while AAP was kept at 7.285μM

HRP was increased to 50μM and AAP to 156.25 μM for the final trial.

Data

Discussion

Most of the day was spent trying to determine concentrations of HRP and AAP that would allow us to see the reaction over the course of 300 seconds. For the final trial, we used the same ones as the other lab group. Tomorrow we will continue with these concentrations, and add AuNPs. This will help us determine whether AuNPs deactivate HRP by interacting with it's active site.