Recombinant Human PDGFB (Ser82 ­ Thr190) was expressed in E. coli and produced using non­animal reagent in an animal­free laboratory.

Description:

Platelet­derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the μ­granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells.

Source:

E. coli

N­terminal Sequence Analysi:

Ser82

Structure / Form:

Disulfide­linked homodimer

Molecular Mass:

12.4 kDa

Activity:

Measured in a cell proliferation assay using NR6R­3T3 mouse fibroblasts. The ED50 for this effect is typically 1.5 ­ 6 ng/mL.

Endotoxin Leve:

<1.0 EU per 1 μg of the protein by the LAL method.

Purity:

>97%, by SDS­PAGE under reducing conditions and visualized by silver stain.

Formulation:

Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. See Certificate of Analysis for details.

Reconstitution:

Reconstitute at 100 μg/mL in sterile 4 mM HCl.

Shipping:

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.