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Oral presentation

There has been considerable recent interest in the roles of vesicle transport proteins
in ciliary biology and regulation of signal transduction. We have previously discovered
RAB23 mutations in Carpenter syndrome, in which patients present with craniofacial and
cardiac malformations, postnatal obesity and tumour formation, and Rab23 regulates
trafficking of hedgehog signalling components between cilia and the cytoplasm. In
the current study we investigated whether Rab23 may also regulate other signalling
pathways. Wnt signalling pathways regulate a variety of developmental processes, yet
the mechanisms of Wnt signal transduction remain unclear. We show that zebrafish deficient
for Rab23 or its GTPase-activating protein, Evi5l, exhibit abnormal heart formation,
which we attribute to a requirement for rab23 in the differentiation of cardiac progenitor
cells. We delineate a Wnt11 signalling pathway that is implicated in cardiomyocyte
differentiation, which acts via phospholipase C, inositol triphosphate receptor, and
protein kinase C, to regulate activator protein 1 (AP-1) transcriptional activity
downstream of c-Jun N-terminal kinase. We also show that Rab23 interacts functionally
with several components of this pathway to regulate transcription by AP-1. Collectively,
these findings identify Rab23 as a novel positive-regulator of non-canonical Wnt11/AP-1
signalling, which may be relevant to cardiovascular disease and stem cell biology.
We also present evidence that Rab23 is dispensible for hedgehog signalling in zebrafish,
and we propose that this reflects previously reported evolutionary differences in
the requirement for cilia in hedgehog signal transduction. Collectively, our findings
suggest that Rab23 may integrate multiple signalling pathways, possibly playing both
cilia-dependent and –independent roles.