Cryopreserved and thawed semen of L. tigrinus exhibited a marked decrease on sperm motility (mean 55%) and progressive motility (mean 34,7%). In L. pardalis, semen evaluation showed a slighter decrease on sperm motility (mean 21%) and progressive motility (mean 19%). Both semen species presented elevation on the percentage of major defects (mean 52% and 30,5%, respectively) due to the increase of acrosomal injuries. Sperm contamination by urine was a remarkable factor on L. tigrinus (53% of the ejaculates) and L. pardalis (59% of the ejaculates) that occasioned a high incidence of bent and coiled tails (23,4%--tigrinus, and 13,3%--ocelots) in addition to cause loss of spermatic resistance to face cryopreservation/thawing stress. Evaluation of spermatic cell ultrastructure revealed that, after cryopreservation, semen of two species showed severe acrosomal damages, which might be a limiting factor for the cryopreserved semen fertility.

In conclusion, the results appointed that there were not any evident differences between the two cryodiluents for both species.