Clone Authentication

clone Authentication

Clone Authentication With A Detailed Phenotypic Profile In Just 3 Days

The Valita™ID assay applies ChemStress Fingerprinting for rapid clone authentication with greater resolution. Valita™ID is designed to confirm a cell line's identity and provides increased regulatory assurance, with a detailed phenotypic profile in just 3 days. Valita™ID carries out cell clone identification at a lower cost, and in a quicker time to produce a result with greater resolution. Current methods of clone authentication are based on outsourcing genomics analysis. Karyotyping, AFLP detection, Short tandem repeat testing and single nucleotide polymorphism testing are all common methods.

Ease of Use

Shortening of drug development time;

Resolution

A detailed phenotypic profile in 3 days; Greater Resolution

Technology Platform: ChemStress Fingerprinting

The ChemStress plate simulates the biological and environmental stresses a cell clone encounters in a bioreactor. Each of the wells of the ChemStress 96-well plate are pre-coated with multiple small molecule chemicals, which re-create the environmental and biological stresses of a bioreactor. Each chemical has been carefully selected to challenge a particular aspect of a cell clones biochemistry. Some of these challenges include: Nutrient Starvation; Oxidative Stress; pH, Osmolarity and Toxicity. For example, Brefeldin A inhibits protein transport and is a lactone antibiotic.

How To Generate A ChemStress Fingerprint

An individual cell clone is grown in the ChemStress plate for 3 days. After exposure to the chemical stressors, clonal growth and product titre are measured in response to each of the chemical stressors. This is read on a plate reader and analysed using the Valitacell software 'ValitaAPP'. ValitaAPP performs the analysis of a cell clones growth and titre profile using product-specific algorithms. This makes up a clones ChemStresss Fingerprint, and this fingerprint is unique to an individual cell clone or a media batch.

1) An individual cell clone is grown in the ChemStress plate for 3 days and exposed to the numerous small molecule chemicals 2) A clones growth and productivity are measured on a plate reader 3) ValitaAPP software applies product-specific algorithms to analyse the growth and titre data 4) An ID ChemStress fingerprint is generated to determine how stable a clone will be in the bioreactor.