By integrating a high intensity xenon flash lamp and liquid
light guide technology, the Polarstar can cover the wavelength
range from 250 to 740 nm. The Polarstar can read all plate
formats from 6- up to 384-well plates in all 4 measurement
modes and 1536-well plate reading is possible in both
fluorescence and time-resolved mode. The versatile optical
system allows instant switching from top to bottom reading.

Incubation

Endpoint, slow and fast kinetic measurements

Kinetic applications can be defined,
with interval times from 100 ms to several hours. In addition,
there is the possibility to measure where it is important in a
kinetic study. Multi-color detection

The fast filter wheels and the ability to define up to 8 sets of
filter, each with individual gain and measurement timing
parameters, allow analysis of multiple fluorophores in a single
well (FRET, dual-emission, DNA-detection).

Specifications

Technical Specifications

Measurement Principles

Fluorescence
Time-Resolved Fluorescence
Absorbance

Light Source

High-energy xenon flash lamp

Detector

Side window, current type photomultiplier tube

Filters

Two filter wheels for 8 filters each
with your choice of 2 filter pairs

Gain Control

Automatic gain adjustment

Fluorescence Polarization

<5 mP standard deviation at 1
nm fluorescein

Fluorescence Intensity

Limit of detection: 0.9 fmol / well fluorescein
calculated according to the IUPAC standard: 3(SDblank)/slope

Time-Resolved Fluorescence

Limit of detection: 50 amol/well europium
calculated according to the IUPAC standard: 3(SDblank)/slope