Investigation
of Features Related to Androgen Dependency in Prostate Cells Using 1H HRMAS MRS
and Principal Component Analysis

1Cancer Research UK Clinical Magnetic Resonance Research Group, Institute of Cancer Research and Royal Marsden Hospital, Sutton, Surrey, UK

Principal Components Analysis applied to 1H HRMAS MRS of
normal and cancerous prostate cell lines grown with and without androgens to
study the metabolic features characteristic of androgen independence.
Preliminary studies show a large number of peaks that can discriminate between
healthy and cancerous cell lines (P-Cho, Ala and Lac elevated in cancer, Cr and
polyamines elevated in healthy cells), and also that creatine is less abundant
in androgen insensitive cancer cell lines when grown in the absence of
androgen.