>I am looking for a good technique for fixing and wax embeding nematodes.
The nematodes will not be
>whole, only front ends, which should aid the embedding process.
>>Fixattion: Berland's fluid or v. hot alcohol for live worms; storage in
70-80% alcohol.
For wax-embedding see:
Cooper, D.W (1988) The preparation of serial sections of platyhelminth
parasites, with details of the materials and facilites required. Systematic
Parasitolgy 12: 211-229.
David Gibson
from
Dr David I. Gibson
Parasitic Worms Division
Department of Zoology Try:
The Natural History Museum http://www.nhm.ac.uk/
London SW7 5BD
UK
Tel. +44-(0)171-938-9485
Fax. +44-(0)171-938-8754
Internet: dig at nhm.ac.uk