DNA fragments bind to silica membrane in high salt buffer. Cellular metabolite and proteins are removed by a serial of elution- centrifuge steps. Then DNA fragments are eluted in low salt and high ph buffer.
Features
Do not contain poisonous phenol and do not need carrying ethanol precipitation.
Best quality binding solution has no inhibition to down-stream reactions because there is no sodium iodide and perchlorate.
The color of binding buffer DB is yellow, which is convenient for monitoring the pH when melting gel.
The silica membranes in the spin-column come from the world-famous company. The recovery efficiency for short fragments can be achieved 75%.