Useful For

Recovery of Helicobacter
pylori from gastric specimens for antimicrobial susceptibility
testing of the organism (amoxicillin, ciprofloxacin,
clarithromycin, metronidazole and tetracycline are routinely
tested)

Reflex Tests

Test ID

Reporting Name

Available Separately

Always Performed

GID

Bacteria Identification

No, (Bill Only)

No

TISSR

Tissue Processing

No, (BIll Only)

No

MIC

Sensitivity, MIC

No, (Bill Only)

No

SUS

Susceptibility

No, (Bill Only)

No

ISAE

Aerobe Ident by Sequencing

No, (Bill Only)

No

Testing Algorithm

When this test is ordered, the reflex tests may be performed and
charged.

When Helicobacter
pylori is isolated, identification will be confirmed and
susceptibility testing performed. The routine susceptibility panel
includes amoxicillin, ciprofloxacin, clarithromycin, metronidazole,
and tetracycline.

See Helicobacter
pylori Diagnostic Algorithm in Special Instructions

Reporting Name

Helicobacter pylori Culture + Susc

Specimen Type

Varies

Shipping Instructions

Specimen must be received in laboratory within 48 hours of
collection.

Specimen Minimum Volume

0.5 mL or 0.5 × 0.2 × 0.2-cm sized piece of
tissue

Specimen Stability Information

Specimen Type

Temperature

Time

Varies

Refrigerated

48 hours

Reference Values

No growth after 7 days

Susceptibility results are reported as minimum inhibitory
concentration (MIC) in mcg/mL and as susceptible, intermediate, or
resistant according to the Clinical and Laboratory Standards
Institute (CLSI) guidelines.

In some instances an interpretive category cannot be provided
based on available data and the following comment will be included:
"There are no established interpretive guidelines for agents
reported without interpretations."

Susceptible (S):

The "susceptible" category implies that isolates are inhibited
by the usually achievable concentrations of antimicrobial agent
when the dosage recommended to treat the site of infection is used,
resulting in likely clinical efficacy.

Intermediate (I)

The "intermediate" category includes isolates with antimicrobial
agent minimum inhibitory concentrations (MICs) that approach
usually attainable blood and tissue levels, and for which response
rates may be lower than for susceptible isolates.

Note: The
intermediate category implies clinical efficacy in body sites where
the drugs are physiologically concentrated or when a higher than
normal dosage of a drug can be used. This category also includes a
buffer zone, which should prevent small, uncontrolled, technical
factors from causing major discrepancies in interpretations,
especially for drugs with narrow pharmacotoxicity margins.

Resistant (R)

The "resistant" category implies that the isolates are not
inhibited by the usually achievable concentrations of the agent
with normal dosage schedules and/or that demonstrate MIC that fall
in the range where specific microbial resistance mechanisms are
likely, and clinical efficacy of the agent against the isolate has
not been reliably shown in treatment studies.

Day(s) and Time(s) Performed

Monday through Sunday; Continuously

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test uses a standard method. Its performance characteristics
were determined by Mayo Clinic in a manner consistent with CLIA
requirements. This test has not been cleared or approved by the
U.S. Food and Drug Administration.