[Histonet] embedding protocol for embryos

From:

Vicki Hakim

hi all
does any one know a good protocol for embedding young mouse embryos( E-8/9)? the embryos are used for mRNA in-situ hybridizations. i have been trying different washing protocols and the tissue is always porous. if anyone could comment on fixation time and the time recommended for such small tissue to be in the melted paraffin that would be great.
thanks
vicki ‎
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