Instantaneous - Depth measured below water line or instantaneous water body surface

Parameters

BODC CODE

Rank

Units

Short Title

Title

ADEPZZ01

1

Metres

DepBelowSurf

Depth below surface of the water body

ALLOHPP1

1

Nanograms per litre

alloxanthin_water>GF/F_HPLC

Concentration of alloxanthin {CAS 28380-31-6} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

BBCAHPP1

1

Nanograms per litre

BB-carotene_water>GF/F_HPLC

Concentration of beta,beta-carotene {beta-carotene CAS 7235-40-7} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

BOTTFLAG

1

Dimensionless

C22_flag

Sampling process quality flag (BODC C22)

BUTAHPP1

1

Nanograms per litre

19'-butanoyloxyfucoxanthin_water>GF/F_HPLC

Concentration of 19'-butanoyloxyfucoxanthin per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

CHLBHPP1

1

Nanograms per litre

chl-b_water>GF/F_HPLC

Concentration of chlorophyll-b {chl-b CAS 519-62-0} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

CLC2HPP1

1

Nanograms per litre

chl-c2_water>GF/F_HPLC

Concentration of chlorophyll-c2 {chl-c2} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

CLC3HPP1

1

Nanograms per litre

chl-c3_water>GF/F_HPLC

Concentration of chlorophyll-c3 {chl-c3} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

CPHLFLP1

1

Milligrams per cubic metre

chl-a_water>GF/F_fluor

Concentration of chlorophyll-a {chl-a CAS 479-61-8} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and fluorometry

CPHLFLP4

1

Milligrams per cubic metre

chl-a_water>0.2um_fluor_sum_frac

Concentration of chlorophyll-a {chl-a CAS 479-61-8} per unit volume of the water body [particulate >0.2um phase] by filtration, acetone extraction and fluorometry and summation of size-fractionated values

CPHLHPP1

1

Milligrams per cubic metre

chl-a_water>GF/F_HPLC

Concentration of chlorophyll-a {chl-a CAS 479-61-8} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

DIADHPP1

1

Nanograms per litre

diadinoxanthin_water>GF/F_HPLC

Concentration of diadinoxanthin {CAS 18457-54-0} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

DVCAHPP1

1

Nanograms per litre

DVchl-a_water>GF/F_HPLC

Concentration of divinyl chlorophyll-a per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

DVCBHPP1

1

Nanograms per litre

DVchl-b_water>GF/F_HPLC

Concentration of divinyl chlorophyll-b per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

FIRSEQID

1

Dimensionless

FireSeqNo

Bottle firing sequence number

FUCXHPP1

1

Nanograms per litre

fucoxanthin_water>GF/F_HPLC

Concentration of fucoxanthin {CAS 3351-86-8} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

HEXOHPP1

1

Nanograms per litre

19'-hexanoyloxyfucoxanthin_water>GF/F_HPLC

Concentration of 19'-hexanoyloxyfucoxanthin {CAS 60147-85-5} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

PERIHPP1

1

Nanograms per litre

peridinin_water>GF/F_HPLC

Concentration of peridinin {CAS 33281-81-1} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

ROSPOSID

1

Dimensionless

RosPos

Bottle rosette position identifier

SAMPRFNM

1

Dimensionless

SampRef

Sample reference number

SCHLFLPC

1

Milligrams per cubic metre

chl-a_water_2-5um_fluor

Concentration of chlorophyll-a {chl-a CAS 479-61-8} per unit volume of the water body [particulate 2-5um phase] by filtration, acetone extraction and fluorometry

SCHLFLPF

1

Milligrams per cubic metre

chl-a_water_0.2-2um_fluor

Concentration of chlorophyll-a {chl-a CAS 479-61-8} per unit volume of the water body [particulate 0.2-2um phase] by filtration, acetone extraction and fluorometry

SCHLFLPO

1

Milligrams per cubic metre

chl-a_water>10um_fluor

Concentration of chlorophyll-a {chl-a CAS 479-61-8} per unit volume of the water body [particulate >10um phase] by filtration, acetone extraction and fluorometry

SCHLFLPX

1

Milligrams per cubic metre

chl-a_water_5-10um_fluor

Concentration of chlorophyll-a {chl-a CAS 479-61-8} per unit volume of the water body [particulate 5-10um phase] by filtration, acetone extraction and fluorometry

VILXHPP1

1

Nanograms per litre

violaxanthin_water>GF/F_HPLC

Concentration of violaxanthin {CAS 126-29-4} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

ZEOXHPP1

1

Nanograms per litre

zeaxanthin_water>GF/F_HPLC

Concentration of zeaxanthin {CAS 144-68-3} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

Definition of Rank

Rank 1 is a one-dimensional parameter

Rank 2 is a two-dimensional parameter

Rank 0 is a one-dimensional parameter describing the second dimension of a two-dimensional parameter (e.g. bin depths for moored ADCP data)

Open Data supplied by Natural Environment Research Council (NERC)

Niskin Bottle

The Niskin bottle is a device used by oceanographers to collect subsurface seawater samples. It is a plastic bottle with caps and rubber seals at each end and is deployed with the caps held open, allowing free-flushing of the bottle as it moves through the water column.

Standard Niskin

The standard version of the bottle includes a plastic-coated metal spring or elastic cord running through the interior of the bottle that joins the two caps, and the caps are held open against the spring by plastic lanyards. When the bottle reaches the desired depth the lanyards are released by a pressure-actuated switch, command signal or messenger weight and the caps are forced shut and sealed, trapping the seawater sample.

Lever Action Niskin

The Lever Action Niskin Bottle differs from the standard version, in that the caps are held open during deployment by externally mounted stainless steel springs rather than an internal spring or cord. Lever Action Niskins are recommended for applications where a completely clear sample chamber is critical or for use in deep cold water.

Clean Sampling

A modified version of the standard Niskin bottle has been developed for clean sampling. This is teflon-coated and uses a latex cord to close the caps rather than a metal spring. The clean version of the Levered Action Niskin bottle is also teflon-coated and uses epoxy covered springs in place of the stainless steel springs. These bottles are specifically designed to minimise metal contamination when sampling trace metals.

Deployment

Bottles may be deployed singly clamped to a wire or in groups of up to 48 on a rosette. Standard bottles have a capacity between 1.7 and 30 L, while Lever Action bottles have a capacity between 1.7 and 12 L. Reversing thermometers may be attached to a spring-loaded disk that rotates through 180° on bottle closure.

Data Acquisition and Analysis

Total chlorophyll was analysed on samples collected from both the CTD rosette sampler and from a small number of underway samples collected from the ship's non-toxic water supply. Water-column sampling during AMT13 concentrated around 5 or 6 light depths from the predawn CTD cast (~0200 - 0400h local time), with a reduced set of depths from the late morning 'optics' cast (1100h local time).

Water samples from the 5-6 main light depths were collected from CTD bottles and underway samples from the ship's non-toxic supply and these samples were filtered on 25 mm GFF filters. Filters were extracted in 90% acetone for 10-24 hrs and total chlorophyll-a measured with a TD-700 Turner Designs fluorometer following the procedure of Welschmeyer (1994) which minimises interference by chlorophyll-b. The fluorometer was calibrated with dilutions of a solution of pure chlorophyll-a (Sigma, UK) in 90% acetone, the concentration of which was determined spectrophotometrically after the cruise.

Size-fractionation of chlorophyll was carried out for samples collected on the pre-dawn cast. Size-fractionated chlorophyll distribution was determined by serial filtration onto 10, 5, 2 and 0.2 µm polycarbonate membrane filters (Poretics, UK). The filters were treated identically to the GF/F used for total chlorophyll and were soaked in 10-ml 90% acetone (HPLC grade) for 10-24 hours prior to reading on the bench fluorometer.

References Cited

Welschmeyer N.A., 1994. Fluorometric analysis of chlorophyll-a in the presence of chlorophyll-b and phaeopigments. Limnology and Oceanography, 39, 1985-1992.

Instrumentation Description

Not relevant to this dataset.

BODC Data Processing Procedures

Data were submitted to BODC in an Excel spreadsheet and were accessioned under BODC archive SOC040010. Sample metadata provided (CTD cast, date, time, lat/lon and depth) were checked against information held in the database. There were no discrepancies noted.

Parameter codes defined in BODC parameter dictionary were assigned to the variable. The units supplied in the spreadsheet were mg m -3 , which match the Parameter Dictionary units so no unit conversions were necessary.

Data loaded into BODC's database without any changes applied.

A parameter mapping table is provided below;

Originator's Parameter

Units

Description

BODC Parameter Code

Units

Comments

Chlorophyll-a

mg m -3

Concentration of chlorophyll-a {chl-a} per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and fluorometry

CPHLFLP1

mg m -3

n/a

0.2-2µm

mg m -3

Concentration of chlorophyll-a {chl-a} per unit volume of the water body [particulate 0.2-2µm phase] by filtration, acetone extraction and fluorometry

SCHLFLPF

mg m -3

n/a

2-5µm

mg m -3

Concentration of chlorophyll-a {chl-a} per unit volume of the water body [particulate 2-5µm phase] by filtration, acetone extraction and fluorometry

SCHLFLPC

mg m -3

n/a

>5µm

mg m -3

Concentration of chlorophyll-a {chl-a} per unit volume of the water body [particulate >5µm phase] by filtration, acetone extraction and fluorometry

SCHLFLPA

mg m -3

n/a

5-10µm

mg m -3

Concentration of chlorophyll-a {chl-a} per unit volume of the water body [particulate 5-10µm phase] by filtration, acetone extraction and fluorometry

SCHLFLPX

mg m -3

n/a

>10µm

mg m -3

Concentration of chlorophyll-a {chl-a} per unit volume of the water body [particulate >10µm phase] by filtration, acetone extraction and fluorometry

SCHLFLPO

mg m -3

n/a

Total

mg m -3

Concentration of chlorophyll-a {chl-a} per unit volume of the water body [particulate >0.2µm phase] by filtration, acetone extraction, fluorometry and summation of size-fractionated values

CPHLFLP4

mg m -3

n/a

Data Quality Report

The data originator removed any suspect data before submission to BODC.

Problem Report

Not relevant to this dataset.

AMT13 pigment composition by HPLC analysis from CTD bottle samples

Data Acquisition and Analysis

Fresh seawater samples from upto five light depths were collected from 57 stations during the cruise. The main sampling was conducted on the pre-dawn cast each day with a reduced set of samples from the midday cast. All samples and replicates were stored at -80 °C and were returned to SOC in liquid-N for analysis.

Instrumentation Description

Section not relevant.

BODC Data Processing Procedures

Data were submitted to BODC in Microsoft Excel spreadsheet format and saved to the archive with reference SOC040134. The file was provided to BODC with CTD cast number, date, and bottle depth as metadata.

Sample metadata were checked against information held in the database - there was one minor depth discrepancy. Data were provided from a sample collected from 160m during cast 21, but no bottle was fired at this depth instead bottles were fired at 150m and the data were loaded against this depth.

Parameter codes defined in BODC parameter dictionary were assigned to the variables as shown in the table below. Values were multiplied by one thousand where appropriate for conversion into nanograms per litre. Cells where a value was logged as 'Present' were changed to 0 and the flag '<' was added to show values were below the detection level of the instrument. Column headings were substituted for the appropriate BODC parameter code except for Tpigm and Tchla, which were derived from the summation of all pigment values and ChlA and Dv ChlA values repectively. These derived values were not loaded into the database.

Data loaded into BODC's database without any changes.

Originator's Parameter

Units

Description

BODC Parameter Code

Units

Comments

Chl c3

mg m -3

Concentration of chlorophyll-c3 {chl-c3} per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

CLC3HPP1

ng l -1

Unit conversion x1000 applied

Chl c2

mg m -3

Concentration of chlorophyll-c2 {chl-c2} per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

CLC2HPP1

ng l -1

Unit conversion x1000 applied

Peridinin

mg m -3

Concentration of peridinin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

PERIHPP1

ng l -1

Unit conversion x1000 applied

19'But

mg m -3

Concentration of 19'-butanoyloxyfucoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

BUTAHPP1

ng l -1

Unit conversion x1000 applied

Fucox

mg m -3

Concentration of fucoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

FUCXHPP1

ng l -1

Unit conversion x1000 applied

19'Hex

mg m -3

Concentration of 19'-hexanoyloxyfucoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

HEXOHPP1

ng l -1

Unit conversion x1000 applied

Violax

mg m -3

Concentration of violaxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

VILXHPP1

ng l -1

Unit conversion x1000 applied

Diadinox

mg m -3

Concentration of diadinoxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

DIADHPP1

ng l -1

Unit conversion x1000 applied

Allox

mg m -3

Concentration of alloxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

ALLOHPP1

ng l -1

Unit conversion x1000 applied

Zeax

mg m -3

Concentration of zeaxanthin per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

ZEOXHPP1

ng l -1

Unit conversion x1000 applied

Dv ChlB

mg m -3

Concentration of divinyl chlorophyll-b per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

DVCBHPP1

ng l -1

Unit conversion x1000 applied

Chl B

mg m -3

Concentration of chlorophyll-b per unit volume of the water body [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

CHLBHPP1

ng l -1

Unit conversion x1000 applied

Dv ChlA

mg m -3

Concentration of divinyl chlorophyll-a per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

DVCAHPP1

ng l -1

Unit conversion x1000 applied

ChlA

mg m -3

Concentration of chlorophyll-a {chl-a} per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

CPHLHPP1

mg m -3

n/a

B-Carot

mg m -3

Concentration of beta-carotene {beta,beta-carotene} per unit volume of the water column [particulate >GF/F phase] by filtration, acetone extraction and high performance liquid chromatography (HPLC)

BCARHPP1

ng l -1

Unit conversion x1000 applied

Data Quality Report

The following values were reported as suspect by the originator and were flagged 'L' in the database: AMT13_30, 12m: Value for DV ChlA too low. AMT13_65, 52m: Value for DV ChlA too high.

Problem Report

The Atlantic Meridional Transect - Phase 2 (2002-2006)

Who was involved in the project?

The Atlantic Meridional Transect Phase 2 was designed by and implemented by a number of UK research centres and universities. The programme was hosted by Plymouth Marine Laboratory in collaboration with the National Oceanography Centre, Southampton. The universities involved were:

University of Liverpool

University of Newcastle

University of Plymouth

University of Southampton

University of East Anglia

What was the project about?

AMT began in 1995, with scientific aims to assess mesoscale to basin scale phytoplankton processes, the functional interpretation of bio-optical signatures and the seasonal, regional and latitudinal variations in mesozooplankton dynamics. In 2002, when the programme restarted, the scientific aims were broadened to address a suite of cross-disciplinary questions concerning ocean plankton ecology and biogeochemistry and the links to atmospheric processes.

The objectives included the determination of:

how the structure, functional properties and trophic status of the major planktonic ecosystems vary in space and time

how physical processes control the rates of nutrient supply to the planktonic ecosystem

how atmosphere-ocean exchange and photo-degradation influence the formation and fate of organic matter

The data were collected with the aim of being distributed for use in the development of models to describe the interactions between the global climate system and ocean biogeochemistry.

When was the project active?

The second phase of funding allowed the project to continue for the period 2002 to 2006 and consisted of six research cruises. The first phase of the AMT programme ran from 1995 to 2000.

Brief summary of the project fieldwork/data

The fieldwork on the first three cruises was carried out along transects from the UK to the Falkland Islands in September and from the Falkland Islands to the UK in April. The last three cruises followed a cruise track between the UK and South Africa, only deviating from the traditional transect in the southern hemisphere. During this phase the research cruises sampled further into the centre of the North and South Atlantic Ocean and also along the north-west coast of Africa where upwelled nutrient rich water is known to provide a significant source of climatically important gases.

Please note: the supplied parameters may not have been sampled from all the bottle firings described in the table above. Cross-match the Sample Reference Number above against the SAMPRFNM value in the data file to identify the relevant metadata.