Product Description

Norgen’s Yeast Genomic DNA Isolation Kit is designed for the rapid preparation of genomic DNA from a few cellsup to 1 x 108 viable yeast cells (between 0.5 and 1 mL of culture).Purification is based on spin column chromatography using Norgen’s patented resin as the separation matrix.

Briefly, the yeast are pelleted and lysed, and the released genomic DNA is bound to Norgen's column in the presence of Binding Solution (BIND). Under these conditions only the genomic DNAwill bind to Norgen's resin whilemost of thecontaminating RNA and cellular proteinaceous components are removed in the flowthrough. The bound DNAis then washedto remove any remaining impurities(WASH). Lastly, the purified yeast genomic DNA is eluted into 100- 200µL of the provided Elution Buffer or water (ELUTE). Please see procedure flowchart to the right.

The Yeast Genomic DNA Isolation Kit allows for the isolation of genomic DNA from yeast and other cultured fungi.Typical purification yields will vary depending on the cell density of the yeast or fungal culture and species. Purified yeast genomic DNA can be used in anumberof downstream applications includingPCR and Southern blot analysis.

This kit is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.

FEATURES AND BENEFITS

Fast and easy processing - Rapid spin-column format allows for the processing of multiple samples in 90 minutes, and the 96-well plates can also be processed in 90 minutes.

High binding capacity of columns - The binding capacity of the columns in the Yeast Genomic DNA Isolation Kit is 25 µg.

Specifications

Figure 1.Isolation of High Quality Genomic DNA. Genomic DNA was isolated from 1 mL of overnight yeast culture (107 cells) using Norgen’s Yeast Genomic DNA Isolation Kit and a leading competitors kit. Ten microliters of the 200 µL purified DNA were run on a 0.9% agarose gel.Note the quality, consistency and yield of the DNA isolated using Norgen’s kit (Lanes 1 - 4) as compared to the DNA isolated using the competitor’s kit (Lanes 5 and 6).Lane M is Norgen’s MidRanger 1 kb DNA Ladder. The yield of the isolation is summarized in Figure 2.

Figure 2.High Yield Purification. Genomic DNA was isolated from 1 mL of overnight yeast culture (107 cells) using Norgen’s Yeast Genomic DNA Isolation Kit and a competitors kit.Aside from the consistency and integrity of DNA isolated as shown in Figure 1, Norgen’s kit was found to have a significantly higher yield of DNA than the competitor’s kit. Quantification was performed using agarose gel densitometry.

APPLICATIONS

Kit Specifications - Spin Columns

Maximum Input

1 x 108 yeast cells

Average Yield*

Up to 10 µg

Column Binding Capacity

25 µg

Time to Complete 10 Purifications

1.5 hours

* Average yield will vary due to cell density of the yeast or fungal culture, the growth conditions and the species.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.