アプリケーション

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション

Abreviews

特記事項

IHC-Fr

Use at an assay dependent concentration.

IP

Use at an assay dependent concentration.

WB

Use a concentration of 5 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ELISA

Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

IHC-P

Use a concentration of 4 - 8 µg/ml.

ターゲット情報

機能

Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity.