FCS Express: Histogram Subtraction

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Hopefully, when you are analyzing immunofluorescence data, there is a
large difference between your control and positive population, and it
is easy to distinguish between them. When the positives and controls
are close together, the process of dividing positives from negatives
can become less of a science and more of an art. FCS Express helps you
put the science back again.

In 1988, Roy Overton dealt with the issue of
mathematically teasing apart positive and negative populations
(Cytometry 9:619-626). FCS Express implements his solutions with a few
clicks of the mouse. Simply tell FCS Express which file is the sample
and which file is the control and FCS Express will automatically
calculate the percentage positive by four different methods, allowing
you to see how the analysis technique can affect your results.

In addition, you can specify a control histogram on
your plot and have our statistics automatically calculate, directly on
the plot, the percent positive.