For2A-3X-mEGFP
localizes to the cell
cortex as discrete dynamic spots. The localization to the cortex is independent of actin. But linear motility of these dots is dependent on actin. Images were acquired on a TIRF microscope.

For2A-3XmEGFP
(magenta) generates actin filaments (labeled with Lifeact-mCherry, cyan) de novo and along pre-existing actin filaments at the cell cortex. Images were acquired with a dual view TIRF system.