Removing important sample data from Millipore’s Sterivex filter units present a unique set of problems for researchers, however the DNeasy PowerWater Sterivex Kit is the only kit available to provide DNA from these filters without enzymes or harsh organic chemicals. Microbes are released from Sterivex filter units utilizing a novel Cell Release Solution that eliminates extensive incubation times or breaking the casing around the membrane. With Inhibitor Removal Technology, high-quality DNA isolation is possible, even when the original sample was highly contaminated. In 40 minutes, DNA can be purified from all types of water samples.

This kit is recommended for use with Millipore catalog # SVGPL10RC Sterivex GP Filter units, but is also compatible with other Sterivex Filter units.

The DNeasy PowerWater Sterivex Kit was previously sold by MO BIO as the PowerWater Sterivex DNA Kit.

Using the DNeasy PowerWater Sterivex Kit, ready-to-use DNA is obtained for any downstream application in just 40 minutes. The protocol includes cell lysis and DNA purification. Other protocols using enzymatic in-unit digestion and phenol/chloroform extraction take longer than 20 hours to complete. These protocols include long incubation, enzymatic digestion and purification protocol times. The DNeasy PowerWater Sterivex Kit provides cell lysis through bead-based homogenization, speeding up the process which provides faster results.

Less RNA carryover and higher yields of high molecular weight and dsDNA can be seen in DNA obtained using the DNeasy PowerWater Sterivex Kit compared with other lysis protocols which use phenol/cholorform extraction.

The DNeasy PowerWater Sterivex Kit removes all PCR inhibitors from DNA allowing greater precision in downstream applications. DNA purified through an in-unit digestion protocol followed by phenol/chloroform extraction failed to amplify without a 1:10 dilution.

Procedure

To begin the DNeasy PowerWater Sterivex Kit protocol, Sterivex units are treated and incubated with cell release solution. Units are then treated with lysis buffer, followed by mixing and removing lysate for homogenization in a 5 ml bead beating tube. Total genomic DNA is captured after protein and inhibitor removal steps on a silica binding column using a vacuum manifold. The binding column membrane is then washed, resulting in pure, high-quality DNA being eluted for downstream applications.