In total, nine Stewart/Stuart monarchs ruled Scotland alone from 1371 until 1603. The last ruler of Scotland alone was James VI, who in 1603 became the first dual monarch of England and Scotland. Following the Glorious Revolution in 1688, two Stuart queens ruled the isles: Mary II and Anne. Both were the Protestant daughters of James VII and II by his first wife. They were the great-grandchildren of James VI and I. Their father had converted to Catholicism and his new wife gave birth to a son in 1688, who was brought up a Roman Catholic and preceded his half-sisters; so James was deposed by Parliament in 1689, in favour of his daughters. But neither had any children who survived to adulthood, so under the terms of the Act of Settlement 1701 and the Act of Security 1704, the crown passed to the House of Hanover on the death of Queen Anne in 1714.

The name "Stewart" derives from the political position of office similar to a governor, known as a steward. It was originally adopted as the family surname by Walter Stewart, 3rd High Steward of Scotland, who was the third member of the family to hold the position. Prior to this, family names were not used, but instead they had patronyms defined through the father; for example the first two High Stewards were known as FitzAlan and FitzWalter respectively. The gallicised spelling was first borne by John Stewart of Darnley after his time in the French wars. During the 16th century, the French spelling Stuart was adopted by Mary, Queen of Scots, when she was living in France. She sanctioned the change to ensure the correct pronunciation of the Scots version of the name Stewart, because retaining the letter "w" would have made it difficult for French speakers, who followed the Germans in usually rendering "w" as /v/. The spelling Stuart was also used by her second husband, Henry Stuart, Lord Darnley; he was the father of James VI and I, so the official spelling Stuart for the British royal family derives from him.

Principal members of the house of Stuart following the 1603 Union of the Crowns.

Both Mary, Queen of Scots, and Lord Darnley had strong claims on the English throne, through their mutual grandmother, Margaret Tudor. This eventually led to the accession of the couple's only child James as King of Scotland, England, and Ireland in 1603. However, this was a Personal Union, as the three Kingdoms shared a monarch, but had separate governments, churches, and institutions. Indeed, the personal union did not prevent an armed conflict, known as the Bishops' Wars, breaking out between England and Scotland in 1639. This was to become part of the cycle of political and military conflict that marked the reign of Charles I of England, Scotland and Ireland, culminating in a series of conflicts known as the War of the Three Kingdoms. The trial and execution of Charles I by the English Parliament in 1649 began 11 years of republican government known as the English Interregnum. Scotland initially recognised the late King's son, also called Charles, as their monarch, before being subjugated and forced to enter Cromwell's Commonwealth by General Monck's occupying army. During this period, the principal members of the House of Stuart lived in exile in mainland Europe. The younger Charles returned to Britain to assume his three thrones in 1660 as "Charles II of England, Scotland and Ireland", but dated his reign from his father's death eleven years before.

These French and Roman Catholic connections proved unpopular and resulted in the downfall of the Stuarts, whose mutual enemies identified with Protestantism and because James VII and II offended the Anglican establishment by proposing tolerance not only for Catholics but for Protestant Dissenters. The Glorious Revolution caused the overthrow of King James in favour of his son-in-law and his daughter, William and Mary. James continued to claim the thrones of England and Scotland to which he had been crowned, and encouraged revolts in his name, and his grandson Charles (also known as Bonnie Prince Charlie) led an ultimately unsuccessful rising in 1745, ironically becoming symbols of conservative rebellion and Romanticism. Some blame the identification of the Roman Catholic Church with the Stuarts for the extremely lengthy delay in the passage of Catholic emancipation until Jacobitism (as represented by direct Stuart heirs) was extinguished; however it was as likely to be caused by entrenched anti-Catholic prejudice among the Anglican establishment of England. Despite the Whig intentions of tolerance to be extended to Irish subjects, this was not the preference of Georgian Tories and their failure at compromise played a subsequent role in the present division of Ireland.[citation needed]

These monarchs used the title "King/Queen of Great Britain", although that title had no basis in law until the Acts of Union 1707 came into effect on 1 May 1707. Legally, they each simultaneously occupied two thrones, as "King/Queen of England" and "King/Queen of Scotland".

sister of Mary II. daughter of James II & VII. Name of state changed to Great Britain with the political Acts of Union 1707, though family has used title since James I & VI. Died childless, rights pass to House of Hanover.