As a result of the Zika virus outbreak, researchers are accelerating research into the biology of the virus and the pathology of human infection with the goals of understanding the causes of the clinical syndrome, developing reliable detection methods and finding effective treatments.

Why use RNAscope® assay for zika virus research?

RNAscope® ISH, an innovated ISH assay, offers researchers a robust and accurate method to detect even low levels of viral RNA in virtually any tissue samples. The ability to detect the virus within the context of the infected tissue will advance the understanding of the zika viral biology.

Morphologic context - Performed in situ, RNAscope® assay preserves the morphology of the infected tissues, enabling detection of the viral RNA in the morphological context. Miner et al., 2016 demonstrated this in detection of Zika in eyes of mouse fetuses

Versatile probe design for low viral count - When working with zika or other viruses of extremely low copy number, ACD can customize the probe design strategy to offset this challenge; for example by designing probes with more than the typical 20 Z probe pairs.

van der Eijk AA et al detected ZIKA viral RNA with the use of virus-specific RNAscope® probes that revealed staining in the amniotic epithelium and the fetal mesenchymal cells, particularly in the perichondrium.

Goveroet al (2016) used RNAscope® Probe Catalog #467871 to determine Zika viral tropism. RNAscope in situ hybridization (ISH) was applied to detect viral RNA at in cells of the testis at day 7 after infection (Fig 1), day 14 (Fig 2), day 21 (Fig 3) and day 28 (Fig 4). Cells of the testis include mature lumenal sperm, and on cilia layering the inner lumen of the epididymis.

Bhatnagar J et al (2017) used RNAscope Probes 468361 and 469541 (designed against the sense and anti-sense strands) and demonstrated not only detection of the virus but also detection of viral replication. They isolated the viral replication to neuronal cells in fetal brain tissues.

Smith (2017) and a team of researchers at the United States Army Medical Research Institute of Infectious Diseases applied RNAscope 2.5 HD RED to visualize Zika Virus in mouse models. Their goal is to development of well-characterized animal models that recapitulate human disease. RNAscope ISH data was presented throughout the paper in Fig 3 (ZIKV in Cerebrum) . Fig 4 (ZIKV in hippocampus), Fig 5 (ZIKV in spinal cord), Fig 7 (ZIKV in spleen). Using this model, they provide a detailed description of the ZIKV-associated pathologic changes, which mirrors the neuropathogenic properties of ZIKV in humans.

ACD can design RNAscope® ISH probes against unique regions of these publically available sequences or proprietary sequences that you may have. We can design it to the sense or antii-sense strand. Depending on your study, the probe design can target specific gene regions or span across the genome. We will also work with you to design positive and negative controls.

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By checking this box, I agree to receive relevant marketing communications regarding products, industry news, literature, and events from Bio-Techne and its brands:Advanced Cell Diagnostics, R&D Systems, Novus Biologicals, Tocris Bioscience, and ProteinSimple. I understand that I can change my preferences at any time. I further understand that any data provided to Bio-Techne and its brands will be stored, used, and deleted consistent with Bio-Techne’s Privacy Notice