Hi! Eric Parent,
Do you still doing the microsatelite sequencing? Have you got the answer for
this?
FYI, we found that by changing the sequencing chemistry from the terminator
chemistry (BDTv3.1) to primer chemistry solve the problem. We used LI-COR
4200 sequencer with Sequitherm EXCEL II kit from EPICENTRE. This generate up
to 600-base per read.
Anyhow, I'm looking forward for any improved protocol using ABI machine. Do
you have one?
Best regards,
Angela Yong