Background & Aims: Behcet’s disease (BD) is an inflammatory vasculitis of unclear etiology. MCP-1 gene is a member of the C-C chemokines family that is a chemotactic factor for monocytes. The results obtained have shown that the -2518A/G polymorphism of MCP-1 gene is associated with BD. The aim of this study was to evaluate the possible involvement of this polymorphism and Behcet’s disease in the population of the North West of Iran.Materials & Methods: A case-control association study was performed on 72 BD patients and 78 geographically healthy controls using PCR-RFLP. The results were analyzed with chi-square and Fisher exact test.Results: Statistical analysis did not show a significant relationship between this polymorphism and BD. But when gender association analysis was adjusted for gender, the results showed that the frequency of AA genotype in female patients and controls was respectively 16 (66.67%) and 14 (43.75%) with p=0.00 and the frequency of GG genotype in female patients and controls was respectively 0 (0%) and 6 (18.75%) with p=0.00. A allele frequency was 40 (83.33%) in female patients and 40 (62.5%) in female control with p=0.00 and G allele frequency was 8(16.67%) in female patients and 24 (37.5%) in female controls with p=0.00.Conclusion: The present study did not show any significant correlation between polymorphisms -2518A/G MCP-1 gene and Behçet disease in the population of the North West of Iran. However, there was a significant association between this polymorphism and women with Behcet's disease by performing gender-related analysis. High frequency of AA genotypes and low frequency of GG genotype in female patients compared to females control suggest a very strong association between the genotypes of this polymorphism with BD in female population of the North West of Iran. A allele seems to display a protective association, whereas G allele might be a susceptible factor for females suffering BD in North West of Iran.