In 1968, she married her first husband, Carl Kominsky, with whom she relocated to Tucson, Arizona. Their marriage did not last long. However, she retained the surname Kominsky after their split. During this time, she attended University of Arizona, graduating with a BFA in 1971. She was also introduced to Spain Rodriguez and Kim Deitch by former Fugs drummer Ken Weaver, who was living in Tucson at the time. Rodriguez and Deitch introduced her to underground comics, inspiring her to begin writing underground comics herself and to relocate to San Francisco.[1]

Soon after arriving in San Francisco, she was introduced to Robert Crumb by mutual friends, who had noted an uncanny resemblance between her and the coincidentally-named Crumb character Honeybunch Kaminski. Their relationship soon became serious and they began living together not long after. She also fell in with the Wimmen's Comix collective, and contributed to the first few issues of that series. After she and Diane Noomin had a falling out with Trina Robbins and other members of the collective, they started their own title, Twisted Sisters. Kominsky-Crumb has later claimed that a large part of her break with the Wimmen's Comix group was over feminist issues and particularly over her relationship with Robert Crumb, whom Robbins particularly disliked.[1]

Aline married Robert Crumb in 1978.[3] Their daughter Sophie was born in 1981. Since the late 1970s, she and Robert have produced a series of collaborative comics called Dirty Laundry (also known as Aline & Bob's Dirty Laundry), a comic about the Crumb family life. Each of them drew his or her own characters for the comic. Later installments of Dirty Laundry feature contributions by Sophie, who also began producing comics in her teens.

She was featured in a number of scenes in Crumb, the 1994 documentary about the Crumb family.

Since the early 1990s, the family has lived as expatriates in a small French village in the Languedoc-Roussillon region. Aline had long been an avowed Francophile, while Robert had become especially disgusted with American culture. They also believed it would be a better environment for their daughter.

In addition to her comics work, Kominsky-Crumb is also a painter and since moving to France, has focused more on painting and less on producing comics. In February 2007 she released a memoir entitled Need More Love: A Graphic Memoir, a collection of her comics and paintings, along with photographs and autobiographical writings.[6]