When I extract DRR data using "Extract reads in FASTQ/A format from NCBI SRA," for TopHat, I was able to get both a bam file and bam-index file.

However, when I filtered the extracted reads in FASTAQ format using "Filter FASTQ reads by quality score and length" and used the filtered data for TopHat, I was not be able to get the bam-index file and got the following error message, "An error occurred setting the metadata for this dataset Set it manually or retry auto-detection."

I repeated this twice, and I got the same message. Please let me know if you have any suggestion to fix this error.

The file is not empty. When I tried to make the bai file from the link "Set manually and auto-detection" and then "Convert Format," I was able to make a bai file, and I was able to see the bam file using igv.

However, when I was analyzing a different data set (this time I used the original DRR data, not filtered) again, I got the same error (no bai file made) again (I tried twice, and I got the same error message; this situation is exactly the same as my first question). So I tried to make the bai file using the same command (Convert Format). Although this new data set was added to queue four days ago, it has never been processed, and I only get the following message, "This is a new dataset and not all of its data are available yet."

This new data set does not appear to be empty, either, since I can see some sequences and scores in the file.

I have no idea why this data set does not begin to be processed for such a long time. Please give me your suggestion to fix this problem.
Thanks,