Application Information: PHD2 antibody

WB: Use at a dilution of 1/500 - 1/1000 (ECL), a band is at 45-50 kDa, representing HIF prolyl hydroxylase 2. This antibody has been tested in human glioblastoma cell lysates. Optimal dilutions/concentrations should be determined by the end user.

Positive Controls

Human glioblastoma cell lysates

Form Supplied

Liquid

Concentration

1
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Immunogen affinity purified

Purification Note

Affinity purified rabbit sera.

Specifications: PHD2 antibody

Full Name

egl nine homolog 1 (C. elegans)

Background

HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis.

Synonyms

C1orf12, DKFZp761F179, ECYT3, HIFPH2, HPH2, PHD2, SM20, ZMYND6

Cellular Localization

Cytoplasmic

Specificity

This antibody is specific for HIF prolyl hydroxylase 2.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

A synthetic peptide made to an internal fragment of the human protein sequence of HIF prolyl hydroxylase 2. (residues 350-426) [LocusLink ID 54583]