E.cloni Express BL21(DE3) CC Cells 12rxns

Details

Lucigen’s E. cloni EXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors’ preparations of BL21 cells (Figure 1), which is essential for construction of complex expression libraries. The unprecedented transformation efficiency of E. cloni EXPRESS Electrocompetent Cells (> 5 × 109 cfu/µg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment (Figure 2)

EXPRESS pLysS Electrocompetent Cells (> 2 × 108 cfu/µg) also produce T7 lysozyme to repress low-level transcription from the T7 promoter prior to IPTG induction, helping to stabilize inserts coding for deleterious products. EXPRESS pLysE Electrocompetent Cells > 2 × 108 cfu/µg) have a higher level of repression by T7 lysozyme, offering the greatest control over expression of inserts. The ComboPack™ contains 4 transformations each of the E. cloniEXPRESS Competent Cell strains, to determine which works best in expressing your protein of interest.

NOTE: For best results in expressing proteins that may be toxic, we recommend using OverExpress Cells instead of EXPRESS pLysS or pLysE strains. All three E. cloni EXPRESS strains are also available as chemically competent cells. With a transformation efficiency of >1 × 107 cfu/µg and very economical prices, EXPRESS Chemically Competent Cells offer superior value for everyday protein expression work.