Wear suitable protective clothing and gloves.In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).

Product Usage Statements

Storage and Shipping Information

Ship Code

Ambient Temperature Only

Toxicity

Highly Toxic & Carcinogenic / Teratogenic

Hazardous Materials Attention:

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Storage

+2°C to +8°C

Protect from Light

Protect from light

Hygroscopic

Hygroscopic

Do not freeze

Ok to freeze

Special Instructions

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 months at -20°C.

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision

07-August-2009 JSW

Synonyms

Dactinomycin, RNA Polymerase I Inhibitor I, Pol I Inhibitor I

Description

Antineoplastic antibiotic. Inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues. A DNA-intercalator with growth inhibitory activity against certain leukemias and sarcomas. At higher concentrations, DNA polymerase is inhibited. Inhibits cell growth and colony formation in synchronized HeLa cells. A potent inducer of apoptosis in many cell lines; however, actinomycin D has also been shown to suppress programmed cell death of PC12 cells induced by etoposide, an inhibitor of topoisomerase II. Acts as a competitive inhibitor of serine protease from microorganisms. The growth of S. aureus is inhibited by 0.25-0.75 µg/ml, whereas a concentration of 100 µg/ml is required to inhibit E. coli due to permeability differences. Heat resistant and stable between pH 2 and 11; decomposed by strong acids and bases. λmax 441 nm ±2 nm. Note: 1 set = 20 vials x 200 µg.

Form

Red crystalline solid

CAS number

50-76-0

RTECS

AU1575000

Chemical formula

C₆₂H₈₆N₁₂O₁₆

Structure formula

Purity

≥98% by HPLC

Solubility

DMSO (1 mg/ml), Methanol, or Chloroform

Storage

+2°C to +8°C
Hygroscopic
Protect from light

Do Not Freeze

Ok to freeze

Special Instructions

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 months at -20°C.