Author

Abstract

Edible mushroom production has two different stages: the vegetative stage and the fruiting stage. The vegetative phase is performed in a biotechnology laboratory and covers the technique for obtaining “spawns”, which parameters include the multiplication and reproduction of the mycelium. The fruiting phase begins with the appearance of edible mushrooms and includes everything that occurs outside the laboratory. In our country, production of edible mushrooms is limited and generally unknown. So, in this study, the vegetative phase was divided into two stages and conducted in the laboratory. Stage 1: We inoculated spores and implants of the edible mushroom species Agaricus in three synthetic growth mediums: PDA (Potato-Dextrose-Agar), PDY (Potato-Dextrose-Yeast), and MEA (Barley-Biphosphate Potassium-Agar). These were incubated in different growth chambers at three different temperatures (17ºC, 20ºC, and 25ºC). The best mushroom development in terms of micellar growth was obtained in the PDA growth medium. The temperature that contributed most favorably to this development was 17ºC. Stage 2: We re-inoculated implants from the crops of the previous step in four natural substrates (brown rice, barley creole, brown rice combined with horse manure, barley combined with horse manure) and incubated them in growth chambers at three different temperatures. It was observed that the best micellar growth occurred in the natural substrate containing barley creole. Also, the most effective incubation temperature was 20ºC. Thus, we established that the barley grains sold in our city work well as a cheap natural substrate to propagate and produce edible mushroom “seed” of the Agaricus species at a temperature of 20ºC.

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BYU ScholarsArchive Citation

Galeón Alcón, Mercedes Victoria, "Production of the edible mushroom (Agaricus sp.) under laboratory conditions for their multiplication in different culture media" (2009). All Theses and Dissertations. 5366.
https://scholarsarchive.byu.edu/etd/5366