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CRG

Centre for Genomic Regulation (ES)

Prof Xavier Estivill

The CRG is an emerging world-class interdisciplinary research institute, located in the Biomedical Research Park of Barcelona (PRBB), one of the largest biomedical research clusters in southern Europe. The CRG is organized into 5 scientific programmes: Gene Regulation, Stem Cells and Cancer, System Biology, Bioinformatics and Genomics, Cell and Developmental Biology and Genes and Diseases. The core facilities of the institute are state-of-the-art and include that for the Light Microscope, DNA Ultra-sequencing, Mass Spectroscopy, DNA Microarray, High-throughput Screening, FACS, Bioinformatics, and the animal-house.

Contributing personnel - Genomics, Epigenomics and Transcriptomics

Xavier EstivillMark Friedländer

Raquel Rabionet

Marta Vives

The CRG/UPF Proteomics Unit is currently equipped with one new Q-Trap (5500 AB-Sciex) and two stateof- the-art LTQ-Obitrap (XL and Velos; Thermo) instruments, which will be used to identify and quantify selected protein targets or posttranslational modifications. The Unit has broad expertise in sample processing and protein detection and quantification, including techniques such as plasma depletion, glycoprotein-enrichment, strong cation exchange fractionation, and quantification using isobaric tag approaches as well as label-free among others. The Unit can routinely process dozens of samples per day and identify over one thousand proteins in complex mixtures.

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