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PINBOARD SUMMARY

New strains of E. coli will be created to have the capability of utilizing feedstocks derived from agricultural wastes to synthesize gaseous biofuels. The catalytic properties of the key enzymes involved in the butane and isobutane biosynthesis pathways will also be investigated, exploiting magnetic nanoparticles as a means of concentrating, delivering and enhancing the properties of immobilised biocatalysts for their production. In sum, the pathway engineering, synthetic biology, and bioreactor engineering will be carried out with the intention of opening up new routes to butane and isobutane production from widely available and inexpensive feedstocks.