We report an autopsy case of Aspergillus brain abscess that had extended from Aspergillus sinusitis, and attempted to detect the fungus in cytology specimens byFungiflora Y<SUP>®</SUP> and Papanicolaou double staining. An 80-year-old man was found to have right orbital and sphenoidal aspergillosis. Treatment with antifungal agents was ineffective, and a brain abscess was detected by magnetic resonance imaging seven months later. The patient's condition deteriorated and he died. Pus from the brain abscess was used to makes smear and Fungiflora Y<SUP>®</SUP>-Papanicolaou double staining was performed. It was difficult to detect the fungi on screening with the ×10 objective lens of light microscope because of their faint color. However, under a fluorescence microscope the fungal hyphae were apple green and it was very easy to detect them. Thus, Fungiflora Y<SUP>®</SUP>-Papanicolaou double staining seems very useful for detecting fungal hyphae on smear cytology.

Journal

The Journal of the Japanese Society of Clinical Cytology

The Journal of the Japanese Society of Clinical Cytology 36(1), 39-43, 1997