ROCHE MOLECULAR SYSTEM, INC.

ROCHE MOLECULAR SYSTEM, INC. Patent applications

Patent application number

Title

Published

20140134611

DUAL PROBE ASSAY FOR THE DETECTION OF HETEROGENEOUS AMPLICON POPULATIONS - The present invention relates to a method for amplifying and detecting a target nucleic acid in a sample, said target nucleic comprising subgroups with sequence variations and/or individual mutations, wherein an amplification of the nucleic acids in said sample is carried out. This amplification involves a polymerase, primers for generating an amplicon and at least two detectable probes specific for different sequence portions of said amplicon. Detection of the obtained amplicon is brought about by detecting hybridization of the probes mentioned above to said different sequence portions of the amplicon.

05-15-2014

20140127673

DUAL PROBE ASSAY FOR THE DETECTION OF HCV - The present invention relates to a method for amplifying and detecting a target nucleic acid of HCV in a sample, wherein an amplification of the nucleic acids in said sample is carried out. This amplification involves a polymerase, primers for generating an amplicon and at least two detectable probes specific for different sequence portions of said amplicon. Detection of the obtained amplicon is brought about by detecting hybridization of the probes mentioned above to said different sequence portions of the amplicon.

05-08-2014

20140113279

Method for Preventing High Molecular Weight Products During Amplification - The present invention is directed to improved methods for amplifying and detecting a nucleic acid target that may be present in a biological sample comprising a primer pair for generating an amplicon, wherein at least one primer is modified at the 5′ terminus with a polyN sequence being non-complementary to the target sequence, and a detectable probe specific for said amplicon or a DNA binding dye. The formation of high molecular weight products during amplification is prevented or partly or completely suppressed. The invention further provides reaction mixtures and kits comprising said primers for preventing or suppressing the formation of high molecular weight products during amplification and detection of the nucleic acid target.

ALGORITHMS FOR CLASSIFICATION OF DISEASE SUBTYPES AND FOR PROGNOSIS WITH GENE EXPRESSION PROFILING - Methods for generating a normalized expression signal for microarray data based on a theoretical distribution at the unit level to produce a normalized expression signal for the single microarray that is independent of other microarrays. The method typically includes receiving microarray data representing a plurality of probe pairs for a single microarray, determining, for each probe pair, differences between intensities of perfect match (PM) probes and intensities of mismatched (MM) probes, determining a difference signal, D, based on the determined differences, and scaling the difference signal, D, to produce an expression signal, DS. The method also typically includes normalizing the expression signal based on a theoretical distribution at the unit level to produce a normalized expression signal for the single microarray that is independent of other microarrays.

09-19-2013

20130173173

SYSTEMS AND METHODS FOR STEP DISCONTINUITY REMOVAL IN REAL-TIME PCR FLUORESCENCE DATA - Systems and methods for removing jump discontinuities in growth data are provided. A first approximation to a received data set is determined by applying a non-linear regression process to a non-linear function that models the data set to determine parameters, including a step discontinuity parameter. A second approximation to the data set is also determined by applying a regression process to a second non-linear function to determine parameters, including a step discontinuity parameter, of the second function. One of the approximations is selected based on an information coefficient determined for each of the approximations. If a confidence interval for the step discontinuity parameter includes zero, no correction is made, and if includes zero, then a correction is made. For a correction, the portion of the data curve prior to the step change is replaced with appropriate portion of the selected approximation to produce a shift-corrected data set.

07-04-2013

20120196291

Control Nucleic Acids For Multiple Parameters - The present invention concerns the amplification of at least a first and a second target nucleic acid that may be present in at least one fluid sample using an internal control nucleic acid for qualitative and/or quantitative purposes.

08-02-2012

20100091273

Reference Light Source Device - The present invention relates to a system for the optical detection of light from analytical samples, the system comprising an analytical instrument comprising an optical detection unit and a sample block unit for receiving a plurality of analytical samples, the system further comprising a calibration device for calibrating the optical detection unit of the analytical instrument, wherein the calibration device comprises a substrate and at least one electrically powered reference light source coupled to the substrate, said at least one reference light source emitting light being detected by the optical detection unit.

04-15-2010

20100015630

Light Emission Modifiers and Their Uses in Nucleic Acid Detection, Amplification and Analysis - The present invention relates to methods and reagents for modifying the emission of light from labeled nucleic acids for the purpose of real time detection, analysis, and quantitation of nucleic acid sequences, e.g., using singly labeled probes. These methods and reagents exploit advantageous properties of thiazine dyes and diazine dyes. Furthermore, the use of these light emission modifiers in background reduction, nucleic acid duplex stabilization and other uses is also described. Related kits, reaction mixtures and integrated systems are described.

01-21-2010

20100015629

Light Emission Modifiers and Their Uses in Nucleic Acid Detection, Amplification and Analysis - The present invention relates to methods and reagents for modifying the emission of light from labeled nucleic acids for the purpose of real time detection, analysis, and quantitation of nucleic acid sequences, e.g., using singly labeled probes. These methods and reagents exploit advantageous properties of thiazine dyes and diazine dyes. Furthermore, the use of these light emission modifiers in background reduction, nucleic acid duplex stabilization and other uses is also described. Related kits, reaction mixtures and integrated systems are described.

01-21-2010

20100015598

Light Emission Modifiers and Their Uses in Nucleic Acid Detection, Amplification and Analysis - The present invention relates to methods and reagents for modifying the emission of light from labeled nucleic acids for the purpose of real time detection, analysis, and quantitation of nucleic acid sequences, e.g., using singly labeled probes. These methods and reagents exploit advantageous properties of thiazine dyes and diazine dyes. Furthermore, the use of these light emission modifiers in background reduction, nucleic acid duplex stabilization and other uses is also described. Related kits, reaction mixtures and integrated systems are described.

01-21-2010

20090320617

Device and Method for the Adjustment of a Temperature of a Liquid - A device and a method for the adjustment of a temperature of a liquid which is contained in one or more sample vessels are specified, a control unit and a temperature adjustment unit being provided, which acts on the liquid contained in the sample vessels. Furthermore, the control unit is operatively connected to the temperature adjustment unit. The liquid to be analyzed contains absorption elements in order to accelerate the temperature adjustment in the liquid to be analyzed.