'Leave my little sisters out of it!' Khloe Kardashian lashes out after Kendall and Kylie Jenner are accused of underage partying AGAIN

Khloe Kardashian gets very testy when it comes to people saying negatives things about her family.

And once again the 29-year-old reality star unleashed a barrage of angry tweets after her sisters Kendall, 17, and Kylie, 16, were suspected of partying - again - at a nightclub.

The newest accusations came after Kylie posted an Instagram of herself sharing a wobbly group hug with Khloe and Kendall at what looks like a happening scene.

At a concert, not a club: Khloe Kardashian lashed out at people who suspected Kendall and Kylie of partying at another club, insisting they were at a concert

Khloe is shown sandwiched between her younger siblings and leaning over with eyes closed to give Kylie a kiss on the hand.

Kendall is seen smiling into the camera and Kylie, too, has her eyes closed while showing off her bare waist in a short-cropped white T-shirt.

Khloe added fuel by posting a similar although more blurry version on her Twitter account, writing: 'My heart and soul,'

A social networking hailstorm ensued with followers wondering if Khloe had taken her much younger sisters to a nightclub, just days after Kendall and Kylie had been seen at the Troubadour in West Hollywood and prior to that, the 21-and-over Vignette Lounge.

Swaying: Khloe posted this picture of herself having what looks like a grand time with her younger sisters

'Stop assuming the worst': Khloe posted a lengthy rant on her Twitter

Not so - apparently the girls were at
brother-to-be Kanye West's concert at The Staples Centre in downtown
Los Angeles on Saturday night.

Khloe had also complimented the entertainer himself in a separate tweet: 'Kanye you were phenomenal tonight!!!!'

Like a lioness protecting her cubs, Khloe took to Twitter to unleash her frustration on Sunday.

Not a diva: Khloe was spotted hauling her own luggage out of the LAX terminal on Friday following the Las Vegas trip

Jet setter: The 29-year-old socialite opted to wear thigh-high leather boots and a sheet black top for her flight

Here kitty, kitty: The former X Factor host had a moment of vanity with her hand-held Hello Kitty mirror

'So sick and tired of people talking
s*** about my family. Last night I posted a picture of my little sisters
at a CONCERT NOT a club,' Khloe wrote.

'Stop
assuming the worst! Stop trying to make us into something that we
aren't! Its NOT going to happen! Judge us if you must but leave my
little sisters out of it!

'They have enough to deal with as it is. Remember our words are our most painful weapon,' Khloe admonished.

Low key after a late night: The 16-year-old dressed down in a large hooded sweatshirt and leggings

Not afraid to get her hands dirty: Kylie stopped to refuel her car before taking off

Protective: Khloe indicated she's 'sick and tired' of people talking negatively about her family; she's pictured here at TAO Nightclub in Las Vegas with Kim and Kourtney on Friday

Sisters are forever: The 29-year-old reality star posted this picture on her Twitter too, writing: 'BFFs'

'Come for me all you want but LEAVE THEM ALONE!!!!'

Khloe continued: 'If someone isn't what others want them to be, the others become angry.

'Everyone seems to have a clear idea of how other people should live their lives, but none about his or her own.'

Awww: Once she had calmed down from her Twitter tantrum, Khloe posted this picture of her brother Rob and Kourtney's son Mason, writing: 'Life is beautiful'

Once the Twitter tantrum had subsided, Khloe seemed have a little laugh at herself, adding: 'You know when KhloMoney uses Caps during a rant, S*** just got real.'

Khloe then posted a picture of her brother Rob and nephew Mason, writing: 'Life is beautiful.'

She also took time out to plug Sunday's episode of Keeping Up With The Kardashians.

'Happy #KUWTK day!!! Who's excited for tonight?

In the club: Kylie Jenner and friends were at the Troubadour in West Hollywood on October 19 to see their brother Brandon Jenner and his wife Leah perform with their band

Family ties: Kendall Jenner arrived at the Troubadour to support her brother's band, Brandon & Leah

'Sundays are THE BEST because #KUWTK is on!!' she wrote, adding: 'OHHH YEAH!!! #KUWTK tonight.'

Khloe
shared a snapshot of herself with sisters Kim and Kourtney at an event
in Las Vegas, captioning the photo with a simple 'BFFs.'

On
October 19, Kendall and Kylie were spotted at the Troubadour in West
Hollywood, just days after the teen girls were busted leaving the
21-and-over Vignette Lounge.

However,
this time around they didn't have to worry about the legitimacy of
their ID cards, as the girls were at an all-ages show at the legendary
Los Angeles hotspot to support their brother Brandon Jenner and his wife
Leah as they performed.

Kendall and little sister Kylie weren't
the only family members to arrive on the scene, as father Bruce Jenner
was also seen at the show.

Dad's day out: Bruce Jenner made his way through the crowd of the all-ages sold out show

Rules of the night: Brandon & Leah played an all-ages show at the Troubadour, which has strict policies regarding fake IDS

Ladies' night: Kylie Jenner linked arms with a gal pal at her brother's show

Last week, the Jenner girls found themselves in hot water when they were seen at the Vignette Lounge, where they accompanied Khloe and Scott Disick to the club's sex-themed Wax Rabbit night.

The underage teens were caught exiting the night club, which posts a strict ID policy.

The bouncers working that Tuesday night told TMZthat
they checked the IDs of the reality stars before they were allowed
entrance into the bar, which according to their Facebook page, offers a
night of nudity and hip-hop.

Whether or not the girls used fake IDs to gain entrance into the club is currently in dispute.

Mother
Kris claims that the girls didn't doctor their identification cards,
whereas the club manager and staff are claiming that the duo did indeed
use altered cards.

Caught in the act: On October 15, underage teens Kylie and Kendall Jenner were pictured leaving the 21-and-over Vignette nightclub in West Hollywood