miRNA Isolation or Extraction From Plasma and Serum—NucleoSpin miRNA Plasma

Isolate small RNA and DNA from plasma and serum.

Principle/Procedure

The NucleoSpin miRNA Plasma procedure is a simple and fast method to isolate small RNA >15 nt, such as micro RNA (miRNA), from serum or plasma. The DNA can be isolated as well or digested on-column to eliminate interference of miRNA genes with downstream applications.

This proven, efficient silica membrane-based procedure isolates total RNA and DNA from plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time-consuming proteinase digest. The sample material is denatured in Lysis Buffer MLP. The protein is then precipitated by Protein Precipitation Buffer MPP and pelleted by centrifugation. After the removal of protein, the binding conditions for nucleic acids are adjusted by adding isopropanol, allowing small RNA and DNA to bind to the NucleoSpin miRNA Column. Optionally, DNA can be removed by an on-column rDNase digest. The remaining nucleic acids are washed and eluted with minimal amounts of RNase-free water.