ASK1 AntibodyCATALOG NUMBER: 3679

Western blot analysis of ASK1 in 3T3 cell lysate with ASK1 antibody at (A) 1 and (B) 2 μg/mL.

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0.02 mg - $79.00

0.1 mg - $325.00

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Specifications

Properties

Additional Info

Background

Specifications

SPECIES REACTIVITY:

Human, Mouse

TESTED APPLICATIONS:

ELISA, WB

APPLICATIONS:

ASK1 antibody can be used for the detection of ASK1 by Western blot at 1 - 2 μg/mL. 3

USER NOTE:

Optimal dilutions for each application to be determined by the researcher.

POSITIVE CONTROL:

1) Cat. No. 1212 - 3T3 Cell Lysate

IMMUNOGEN:

ASK1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ASK1.

The immunogen is located within amino acids 390 - 440 of ASK1.

HOST SPECIES:

Rabbit

Properties

PURIFICATION:

ASK1 Antibody is affinity chromatography purified via peptide column.

PHYSICAL STATE:

Liquid

BUFFER:

ASK1 Antibody is supplied in PBS containing 0.02% sodium azide.

CONCENTRATION:

1 mg/mL

STORAGE CONDITIONS:

ASK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Background

BACKGROUND:

ASK1 Antibody: Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-α-induced apoptosis. ASK1 is expressed in variety of tissues and cell lines.