Hello, eveyone.
I want to know about how to do TA cloning well. I have just made
a T vector by the following protocol.
pBluscript 10micrograms -(EcoRV digestion) - (Et-OH precipitation)-
-(70degrees centigrade, 2hrs with dTTP in thrmocycler)-purification
But my vectors didn't work well. The sample before ligation (no insert)
could make a lot of white colonies. Maybe to add T residue to pBluscript
was not enough, I think.
Please give me som or ideas advice. Thank you in advance.
Thank you.
--
Shin Yamamoto
h971409d at eds.ecip.nagoya-u.ac.jp