Title (fr)

Publication

Application

Priority

DE 4321904 A 19930701

EP 9402056 W 19940624

Abstract (en)

[origin: CA2142910A1] A method for the purification and separation of nucleic acid mixtures by chromatography comprising adsorbing the nucleic acids to be separated and purified from a solution with a high concentration of salts (ionic strength) and/or a high concentration of alcohol on a substrate and subsequent desorbing from said substrate by means of a solution with lower concentration of salts (ionic strength) characterized in that said nucleic acid mixture - is adsorbed from an aqueous adsorption solution with a high concentration of salts (ionic strength) and with 1 to 50 % by volume of an aliphatic alcohol having a chain length of C1-C5 and/or polyethylene glycol (PEG) and/or hydrophobic inorganic and/or organic polymers and/or organic acid, such as trichloroacetic acid (TCA), on a porous or non-porous mineral substrate of metal oxides and/or mixed metal oxides, silica gel, materials principally consisting of glass, alumina, zeolites, titanium dioxide, zirconium dioxide; - is optionally washed with a washing solution, and thereaf ter - is eluted with a solution with a lower concentration of -salts (ionic strength), and the nucleic acid or nucleic acid fraction obtained is collected.