The Emission Filter Wheel Module for Thermo Scientific™ ArrayScan™ HCA Readers and suitable filters can discriminate the spectra of a fluorochrome better so that there can be more efficient use of specific wavelengths to label and image components of a cell.

Features

Automated specificity—Permits more flexibility in spectral combinations allowing for imaging of more custom fluorochromes. May reduce background and increase signal-to-noise when fitted with appropriate filters

Integrated—Integrated with software and validated for performance for simple setup and customization. Filter turret controlled from protocol software allowing automatic change during plate run

Rapid filter switching—Rapid turret movement with switching time of 300 ms between cubes

Key addressable assays

FRET (Fluorescence Resonance Energy Transfer)—FRET is when a donor color molecule (chromophore), in its excited state, can transfer some of its energy (the donor) to another chromophore resulting in a much brighter signal in the acceptor. It is used in studying protein-protein interactions.

Qdot® nanocrystals—Tiny semiconductor crystals that can be designed to excite and emit at very specific wavelengths. Cells and structures can be labeled with these and structure identified for analysis

Live cell applications —May be used with optical density filters to attenuate light to avoid phototoxicity whenever more spectral discrimination is needed

Specifications

Compatibility

Compatible with other ArrayScan™ Modules for increased functionality

Emission filter wheel

Corresponding emission filters in the six position emission filter wheel to match the combination of one penta-band, one tri-band, one dual-band and two single band dichroics