Biological sciences

Regulation of the feruloyl esterase (faeA) gene from Aspergillus niger

Article Abstract:

The two regulatory systems in Aspergillus niger that affect expression of genes encoding enzymes involved in degradation of cell wall polymers are not sufficient to account for the induction pattern seen for feruloyl esterase (faeA). To find out about involvement of XlnR and CreA in regulation and to show the presence of a third factor a study was carried out. It also was intended to study the nature of the inducing compounds for the new factor and to analyze relationships among the three regulatory factors. The inducing systems let A. niger make feruloyl esterase during growth on xylan and during growth on other ferulic acid-containing cell wall polysaccharides.

Localization of glucose oxidase and catalase activities in Aspergillus niger

Article Abstract:

Immunocytochemical studies have localized the glucose oxidase in Aspergillus niger in the cell wall. In addition, non-denaturating polyacrylamide gel electrophoresis has established the presence of four distinct catalases. Two are constitutive, while the other two are inducible enzymes. These catalases are postulated to play a major role in the protection of glucose oxidase by degrading hydrogen peroxide formed in the cell wall. Theyalso appear to be involved in intracellular detoxification but data is still inconclusive.

Two cellobiohydrolase-encoding genes from Aspergillus niger require D-xylose and the xylanolytic transcriptional activator XlnR for their expression

Article Abstract:

Two Aspergillus niger cellobiohydrolase-encoding genes must have D-xylose and the xylanolytic transcriptional activator XlnR for expression to occur. A. niger is a filamentous fungus. Cellulase-encoding genes are regulated at the transcription level.