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Genomic DNA is extracted from saliva. Direct polymorphism analysis for CYP2D6 gene is performed after (multiplex) PCR and allele-specific primer extension (ASPE) with Luminex Molecular Diagnostics' proprietary universal tag sorting system on the Luminex 100 xMAP platform. A genotype is assigned based on the allele-specific fluorescent signals that are detected.(Unpublished Mayo method)