Abstract

Addition of 400 μM AlCl3 to the culture medium for 72 h has been previously shown to induce perikaryal whorls of intermediatesized filaments in intact mouse NB2a/d1 neuroblastoma cells. Immunoblot analysis demonstrated that in vivo treatment of cells with aluminum induced thede novo appearance of extensively phosphorylated NF-H isoforms in cytoskeletons of undifferentiated cells and increased levels of these isoforms in differentiated cells. Neurofilament subunits isolated from intact cells treated with aluminum were resistant to dephosphorylation in vitro by alkaline phosphatase and to in vitro degradation by endogenous calcium-dependent protease(s). These alterations were accompanied by a greater tendency of neurofilaments to form insoluble aggregates after isolation. These findings demonstrate direct effects of aluminum on neurofilament subunits within intact neuronal cells similar to those previously demonstrated following in vitro exposure of isolated neurofilaments to aluminum.

References

No relevant information is available
If you register references through the customer center, the reference information will be registered as soon as possible.