> Hello!
>> Does anybody have experience in using
>> polyT primers for PCR?
>> What Tm should I use, what are cycling conditions?
>>> polyT: 5' TTTTTTTTTTTTTTTTTTTTTTTC 3'
>> there are 23 dT and at the 3' end C, G or A
>> for this primer, Tm = 54,6
>>> Thanks! Rimantas.Kodzius at sbg.ac.at
-----
In order to answer your question you need to tell us more about your PCR:
What's your template?
What do you expect your product will be? A single specific product or a
library?
What's the other primer like?
m
--
Mon pays ce n'est pas un pays, c'est l'hiver (Vigneault).