ARISA

Primers

Use "primers ITSF (5′-GTCGTAACAAGGTAGCCGTA-3′) and ITSReub (5′-GCCAAGGCATCCACC-3′)", which are, " respectively, complementary to positions 1423 and 1443 of the 16S rRNA and 38 and 23 of the 23S rRNA of E. coli." [1]

Sources:

ARISA Protocol

Version 1

Extracted DNA was used as a template for PCR on the internal transcribed spacer region using the ITS-FEub (5′-GTCGTAACAAGGTAGCCGTA-3′) and ITS-REub (5′-GCCAAGGCATCCACC-3′) primers (Cardinale et al., 2004).

PCR conditions were as follows: initial denaturation at 94 °C for 3 min, followed by 30 cycles of 94 °C for 45 s, 55 °C for 1 min, and 68 °C for 2 min, ending with a final extension at 68 °C for 7 min.