Notes

Region of Interest (Vector NTI Annotation): 5318 - 6121 (ATG to TGA)

Lots of truncated 5' and 3' bases - may want to redo alignment with less trimming/manual comparison. Considering the 198 trimmed 3' bases would allow me to complete the region between the forward and reverse primers (~50 bases between the two) while considering the 195 trimmed 5' bases would allow me to see the end of the ORF (~140 bases to end of ORF).