Choosing Among the Available Enzyme Systems

Every isozyme project begins with a choice of enzymes. Although it is not possible to predict which enzymes will

Figure 4 Formation of dimers in a triploid. A, The subunits can combine nine ways to form dimers with five possible charges. B, Separating these dimers on a gel and staining gives five bands in a ratio of intensities of 1:2:3:2: 1. The most intense band results from co-migration of — 2/—4 and — 3/— 3 heterodimers. All six bands could be visible if the differences in charge among alleles were uneven. Direction of migration is indicated by the arrow.

Figure 4 Formation of dimers in a triploid. A, The subunits can combine nine ways to form dimers with five possible charges. B, Separating these dimers on a gel and staining gives five bands in a ratio of intensities of 1:2:3:2: 1. The most intense band results from co-migration of — 2/—4 and — 3/— 3 heterodimers. All six bands could be visible if the differences in charge among alleles were uneven. Direction of migration is indicated by the arrow.

work with a particular fungal species, some guidance can be obtained from previous research. The best approach is to screen a few isolates on a large number of enzyme systems, then choose those enzymes which give well resolved, easily storable banding patterns. However, choosing which enzymes to try first can be daunting. Enzyme testing results from 27 published surveys plus three unpublished surveys are summarized in Table 1. Among the 82 enzymes listed, 32 never worked and another 20 were successful in less than 50% of the surveys. The most promising enzymes to try in an initial survey would be the 20 enzymes that were tried four or more times and provided useful information in 50% or more of the studies surveyed (indicated in bold in Table 1).

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