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Genome Engineering Facility

The WIMM Genome Engineering Facility is a central service open to the Oxford research community.

We assist researchers in experimental planning and assembly of reagents for the generation of tailor-made experimental model systems. We are highly experienced in the generation of engineered immortalized cell lines, primary cells and stem cells of different origins, and provide a service platform for the generation of transgenic models.

Meet the team

We mainly apply the CRISPR/Cas9 technology for targeted in vivo mutagenesis. Our portfolio ranges from simple NHEJ-based knockout to defined exon excision, from modelling of simple SNPs to insertion of complex knock-in alleles, all in a variety of model systems. In addition, we assemble custom DNA-based targeting constructs in plasmid or BAC format, e.g. as donors in Cas9-mediated HDR experiments or as transgenic constructs for pronuclear injection. For mouse models requiring insertion of larger cassettes, we tend to suggest using Cas9-assisted homologous recombination in embryonic stem cells. The Cas9-assisted targeting is done in collaboration with the MRC WIMM Transgenic Core Facility and the WTCHG transgenics core.

We are not exclusive to MRC WIMM research groups and offer our services also to researchers in other departments of the University of Oxford. If you have a project in mind and would like to discuss the details, require advice on genome engineering issues and molecular cloning technology, or need a detailed quote for your specific project, please feel free to contact us at any time to find out how we can help.