Abstract

Objectives:
Acinetobacter baumannii is an opportunistic pathogen that causes serious infections in humans by colonization of medical devices. The capacity of this pathogen to persist in hospital settings could be due to its ability to form biofilms. In the present study we evaluated the effect of antibodies against one of surface components of A. baumannii on in vitro biofilm formation.

Materials and Methods:
The 1113 bp fragment of Bap (biofilm associate protein)gene from A. baumannii genome was amplified and cloned. The recombinant protein was expressed and purified and used to raise antibodies in mice. Antibody titer was evaluated by ELISA. In vitro biofilm inhibition was evaluated using the mice sera.