2 11:50-12:30 Eduardo Rial CIB Centro Investigaciones Biológicas- CSIC, Madrid. "Oxidative stress, thermogenesis and evolution of the uncoupling proteins" 12:30-13:10 Guillermo Montoya CNIO Centro Nacional Investigaciones Oncológicas, Madrid. "Molecular machines involved in protein folding and DNA unwinding or how to spend ATP for a good cause " 13:30-13:30 Concluding remarks 14:00 Lunch-colloquium (limited seats available) Certificates of attendance will be distributed at the end of the event. Registration required. No registration fee. For registration, please, send an to: (Specify subject: BioForo) providing the following information: Name, professional status (undergradute student, PhD student, professor.), working address, director, and name and address of supervisor (when appropriate), interested in attending lunch (yes/no). INTRODUCTION AND SPEAKERS PROFILE As in previous years, we offer a Special Bioforo meeting in December designed to show the research that our former students are carrying out outside the UPV/EHU. This year our speakers come from different countries (Germany, Switzerland, Spain and USA) and work in different areas in Biology: Bioinformatics, Cell Mechanics, Molecular Biology, Bioenergetics, and Structural Biology. Miguel Andrade He is interested in exploring gene function using computational techniques including new algorithms and the use of existing databases. He has a focus on studying the mechanisms involved in human disease with the goal of facilitating the development of better therapies. The results of his work are often distributed as software or online web tools. Take a look at the group members' publications or at their projects page for more information.

5 Eduardo Rial. His main interest is Mitochondrial Bioenergetics The mitochondrial oxidative phosphorylation embraces the reactions that allow ATP synthesis using the energy made available from substrate oxidation at the respiratory chain. The two processes are coupled through the proton gradient generated during the transfer of electrons from the substrates to oxygen. His group investigates mechanisms that modulate the energetic efficiency of the process. The uncoupling proteins (UCPs) are carriers of the mitochondrial inner membrane whose biological function is to allow a regulated discharge of the proton gradient. There are a large number of processes that appear to involve the UCPs. For example, this energy dissipatory mechanism is used by mammals to maintain body temperature when cold exposed or to burn excess calories ingested with the diet. Since the UCPs catalyze the re-entry of protons into the mitochondrial matrix, they cause an increase in the rate of respiration that leads to a decrease in the production of reactive oxygen species (ROS) and therefore the UCPs are an element of the cellular defenses against oxidative stress. Thus, the uncoupling protein UCP2, which is found in many tissues and organs, is over-expressed in pathological processes in which ROS play an important role (atherosclerosis, cancer, chronic inflammation, etc.). Over the past few years, genes coding for UCPs have been described not only in animals but also in plants and even in unicellular organisms. The ubiquitous presence of the UCPs suggests that physiological uncoupling of oxidative phosphorylation is a general strategy adopted by living organisms to regulate the energetic efficiency and, for example, modulate the mitochondrial production of ROS. The group has been investigating for nearly thirty years the physiological role and the molecular mechanisms of transport and regulation of the uncoupling proteins UCP1 and UCP2. HOME PAGE Selecteds publications: Ortega-Molina A, Efeyan A, Lopez-Guadamillas E, Muñoz-Martin M, Gómez López G, Cañamero M, Mulero F, Pastor J, Martinez S, Romanos E, González- Barroso MM, Rial E, Valverde AM, Bischoff JR, Serrano M [2012] Pten positively regulates brown adipose function, energy expenditure, and longevity. Cell Metab 15, Luévano-Martínez LA, Barba-Ostria C, Araiza-Olivera D, Chiquete-Félix N, Guerrero-Castillo S, Rial E, Georgellis D, Uribe-Carvajal S [2012] A critical tyrosine residue of the mitochondrial oxaloacetate carrier determines its uncoupling protein (UCP)-like function in yeast. Biochem J 443,

6 Rial E, Rodríguez-Sánchez L, Gallardo-Vara E, Zaragoza P, Moyano E, González-Barroso MM [2010] Lipotoxicity, Fatty Acid Uncoupling and Mitochondrial Carrier Function. Biochim Biophys Acta Bioenerg 1797: Redondo-Horcajo M, Romero N, Martínez-Acedo P, Martínez-Ruiz A, Quijano C, Lourenço CF, Movilla N, Enríquez JA, Rodríguez-Pascual F, Rial E, Radi R, Vázquez J, Lamas S [2010] Cyclosporine A-induced nitration of tyrosine 34 MnSOD in endothelial cells: role of mitochondrial superoxide. Cardiovasc Res 87: Delás J, Notari M, Forés J, Pechuan J, Porcar M, Navarro E, Montagud A, Baguena M, Peretó J, Córdoba PF, González-Barroso MM, Rial E, Moya A, Urchueguía J [2009] Yeast cultures with UCP1 uncoupling activity as a heating device. N Biotechnol 26: González-Barroso MM, Rial E [2009] The role of fatty acids in the activity of the uncoupling proteins. Curr Chem Biol 3: Guillermo Montoya Guillermo Montoya was born in Madrid (Spain) in 1967 and obtained his Bachelor degree in Biochemistry from the Universidad del País Vasco in 1990, and his PhD in Chemistry from the Universidad de Zaragoza in He obtained both a European Molecular Biology Organisation (EMBO) and a Federation of European Biochemical Societies (FEBS) Fellowship and moved to the Max Planck-Institut für Biophysik in Frankfurt am Main (Germany), where he worked on membrane protein crystallisation in the group of the Nobel Laureate H. Michel. Montoya later obtained both an EMBO long-term and a Marie Curie Fellowship and spent nine years at the European Molecular Biology Laboratory (EMBL) in Heidelberg (Germany), working in I. Sinning s Group where he focused on the crystallisation of the cytocrome bc1 membrane protein complex and later pioneered the study of the structure of the signal recognition particle (SRP), an essential ribonucleoprotein complex involved in protein targeting. In 1998 he was appointed as Researcher at the Consejo Superior de Investigaciones Científicas (CSIC) and was awarded a Peter und Traudl Engelhorn Foundation Research Fellowship. Since 2003 he has been an Honorary Professor in Biochemistry at the Universidad Autónoma de Madrid and Member of the working group in charge of the design of the biocrystallography beamline at the Spanish Synchrotron (ALBA). Montoya has been Head of the CNIO s Macromolecular Crystallography Group since February 2002 and was acting Director of the Structural Biology and Biocomputing Programme from November 2003 to January In 2009 he was awarded the National Prizes of the Fundación Mutua Madrileña and the Fundación Caja Rural de Granada-Ministerio de Sanidad. Selected Publications:

Doctor of Philosophy in Biochemistry FACULTY OF MEDICAL SCIENCE Naresuan University 73 Doctor of Philosophy in Biochemistry The Biochemistry Department at Naresuan University is a leader in lower northern

Photosynthesis takes place in three stages: Light-dependent reactions Light-independent reactions The Calvin cycle 1. Capturing energy from sunlight 2. Using energy to make ATP and NADPH 3. Using ATP and

Version 1 2015 Module guide International Master Program Cardiovascular Science University of Göttingen Part 1 Theoretical modules Synopsis The Master program Cardiovascular Science contains four theoretical

How many of you have checked out the web site on protein-dna interactions? Example of an approximately 40,000 probe spotted oligo microarray with enlarged inset to show detail. Find and be ready to discuss

Support Program for Improving Graduate School Education Advanced Education Program for Integrated Clinical, Basic and Social Medicine January 27, 2009 Dear Professors (representative) of departments, Subject:

AT&T Global Network Client for Windows Product Support Matrix January 29, 2015 Product Support Matrix Following is the Product Support Matrix for the AT&T Global Network Client. See the AT&T Global Network

Control of Gene Expression (Learning Objectives) Explain the role of gene expression is differentiation of function of cells which leads to the emergence of different tissues, organs, and organ systems

Oxidative Phosphorylation NADH from Glycolysis must be transported into the mitochondrion to be oxidized by the respiratory electron transport chain. Only the electrons from NADH are transported, these

Compartmentalization of the Cell Professor Alfred Cuschieri Department of Anatomy University of Malta Objectives By the end of this session the student should be able to: 1. Identify the different organelles

Biochemistry Major Talk 2014-15 August 14, 2015 Department of Biochemistry The University of Hong Kong Welcome!!!!!!!!!!!!!! Introduction to Biochemistry A four-minute video: http://www.youtube.com/watch?v=tpbamzq_pue&l

Requirements and Guidelines for the Master of Science Degree in Biomedical Engineering Revised 9/06 I. Minimum Entrance Requirements Admission is subject to final approval by the BME Graduate Committee.

1. An autotroph is an organism that a. extracts energy from organic sources b. converts energy from sunlight into chemical energy c. relies on the energy produced by other organisms as an energy source

Biol 205 Exam 1 TEST FORM A Spring 2008 NAME Fill out both sides of the Scantron Sheet. On Side 2 be sure to indicate that you have TEST FORM A The answers to Part I should be placed on the SCANTRON SHEET.

148 Bishop s University 2015/2016 Biochemistry The Biochemistry program at Bishop s is coordinated through an interdisciplinary committee of chemists, biochemists and biologists, providing students with

Weill Graduate School of Medical Sciences of Cornell University Program in Pharmacology Graduate School Curriculum Weill Graduate School of Medical Sciences Partnership between Weill Medical College and

Ph.D. in Bioinformatics and Computational Biology Degree Requirements Credits Students pursuing the doctoral degree in BCB must complete a minimum of 90 credits of relevant work beyond the bachelor s degree;

1. Which of the following is NOT involved in binary fission in prokaryotes? a. Replication of DNA b. Elongation of the cell c. Separation of daughter cells by septum formation d. Assembly of the nuclear

Unit I: Introduction To Scientific Processes This unit is an introduction to the scientific process. This unit consists of a laboratory exercise where students go through the QPOE2 process step by step

Chem 306 Chapter 21 Bioenergetics Lecture Outline III I. HOW IS ATP GENERATED IN THE FINAL STAGE CATABOLISM? A. OVERVIEW 1. At the end of the citric acid cycle, all six carbons of glucose have been oxidized

CURRICULUM VITAE Rosella Visintin Positions: Since 2006 Since July 2005 Assistant professor of SEMM (European School of Molecular Medicine). IFOM-IEO Campus Milan - Italy Junior group leader at the European

Name Period Chapter 12: The Cell Cycle Overview: 1. What are the three key roles of cell division? State each role, and give an example. Key Role Reproduction Growth and development Tissue removal Example

Cell Structure & Function! Chapter 3! The most exciting phrase to hear in science, the one that heralds new discoveries, is not 'Eureka!' but 'That's funny.! -- Isaac Asimov Animal Cell Plant Cell Cell

MCAS Biology Review Packet 1 Name Class Date 1. Define organic. THE CHEMISTRY OF LIFE 2. All living things are made up of 6 essential elements: SPONCH. Name the six elements of life. S N P C O H 3. Elements

Chapter 16 The Citric Acid Cycle Multiple Choice Questions 1. Which of the following is not true of the reaction catalyzed by the pyruvate dehydrogenase complex? A) Biotin participates in the decarboxylation.

Unit 2 Metabolism and Survival Summary 1 Metabolism pathways and their control (a) Introduction to metabolic pathways This involves integrated and controlled pathways of enzymecatalysed reactions within

AP * BIOLOGY GENE REGULATION Teacher Packet AP* is a trademark of the College Entrance Examination Board. The College Entrance Examination Board was not involved in the production of this material. Pictures

CURRICULUM FOR RESEARCH POSTGRADUATE PROGRAMS Master of Philosophy (MPhil) and Doctor of Philosophy (PhD) Programs in Life Science Curriculum for Master of Philosophy (MPhil) Program in Life Science The

Chapter 9 Biotechnology and Recombinant DNA Biotechnology and Recombinant DNA Q&A Interferons are species specific, so that interferons to be used in humans must be produced in human cells. Can you think

Department of Biochemistry & Molecular Biology Two-Year Master s Program in Biochemistry & Molecular Biology A four-semester post-baccalaureate program designed to provide advanced training in the biochemical

in Molecular Biology And Biotechnology National Institute of Molecular Biology and Biotechnology College of Science University of the Philippines Diliman, Quezon City 1101 National Institute of Molecular

Concept 1 - Thinking Practice 1. If the following molecules were to undergo a dehydration synthesis reaction, what molecules would result? Circle the parts of each amino acid that will interact and draw

An Overview of Cells and Cell Research 1 An Overview of Cells and Cell Research Chapter Outline Model Species and Cell types Cell components Tools of Cell Biology Model Species E. Coli: simplest organism

ATIP Avenir Program 2014 Applicant s guide Important dates: - November 29 th 2013: deadline for the online submission, the mailing of the hard copy of the scientific project, and the letters of recommendation

The How this Integrated Technology Platform has impacted the development of R&D Clusters in Galway Jacinta Thornton, PhD Executive Manager, NCBES National Centre for Biomedical Engineering g Science Mission

Cancer cells have escaped from cell cycle controls Cancer cells divide excessively and invade other tissues because they are free of the body s control mechanisms. Cancer cells do not stop dividing when

Protein Trafficking/Targeting (8.1) Lecture 8 Protein Trafficking/Targeting Protein targeting is necessary for proteins that are destined to work outside the cytoplasm. Protein targeting is more complex

UNC CHARLOTTE A doctoral, research-intensive university, UNC Charlotte is the largest institution of higher education in the Charlotte region. The University is comprised of seven colleges and offers 19

DNA Replication & Protein Synthesis This isn t a baaaaaaaddd chapter!!! The Discovery of DNA s Structure Watson and Crick s discovery of DNA s structure was based on almost fifty years of research by other

Energy Production In A Cell (Chapter 25 Metabolism) Large food molecules contain a lot of potential energy in the form of chemical bonds but it requires a lot of work to liberate the energy. Cells need

Biological molecules: All are organic (based on carbon). Monomers vs. polymers: Monomers refer to the subunits that, when polymerized, make up a larger polymer. Monomers may function on their own in some

Fundamentals of Medicine and Dentistry Basic Scientific Principles that All Students Should Know Upon Entering Medical and Dental School at McGill Students entering medical and dental training come from

Biochemistry (Molecular and Cellular) Information Sheet for entry in 2016 What is Biochemistry? The study of living things at the molecular level has undergone tremendous expansion in recent years, leading

Protein Synthesis Protein synthesis is simply the "making of proteins." Although the term itself is easy to understand, the multiple steps that a cell in a plant or animal must go through are not. In order