The possibility of use of Nocardia vaccinii K-8 cells as well as their metabolites for remediation of oil polluted ecosystems was studdied. It was shown that the highest oil destruction degree (94-98 %) in polluted water (2.6 g/L) was achieved in the case of treatment with suspension of N. vaccinii K-8 cells (9.8107 CFU/mL) after 30 days, while surfactant preparation of post fermentative cultural liquid (100300 mL/kg) was more effective for remediation (destruction of 74-83 % of oil) of oil polluted soil (20 g/kg). Furthermore, the introduction of 30 ml of these preparation to the oil polluted sand (0.1 mL of oil/1 g of sand) resulted in detachment of 90 % of oil.