MSc Exit Seminar – Chun Hua Wei- Tuesday, August 7, 2018

August 7, 2018 @ 1:00 pm - 1:30 pm

Tuesday, August 7, 2018 at 1:10 pm SW -403, University of Toronto at Scarborough

Chun Hua Wei (Hasenkampf Lab)

The role of HOP2 in Homologous Recombination in Arabidopsis thaliana

Abstract:

The purpose of this study was to investigate the role of HOP2 protein in non-meiotic cells in Arabidopsis. HOP2 is already known to be important to meiotic chromosome pairing and homologous recombination, yet the role of HOP2 outside of meiosis is far from being fully elucidated. My study focused on the mitotic chromosome events with and without the application of radiation. In the absence of radiation, no fragments and chromatin bridges were found in hop2-1 plants, but they did seem to experience a modest chromosome separation delay. When irradiated, both genotypes had significant decreases of mitotic indices and increases of bridges. The decreases in mitotic indices were comparable for the two genotypes, suggesting they accomplish repair at similar rates. Irradiated hop2-1 had significantly more mis-repaired breaks, as determined by the bridges. My findings suggest that HOP2 is also important for the fidelity of the exchange process in non-meiotic HR repair.