Johns Hopkins uses hen egg white lysozyme for its intro biochem
lab. Easy to make a whole bunch of it, obvious where it comes
from, and easy to assay in a biologically meaningful (as well
as very cool) way--by looking at the turbidity of a
solution of bacteria that have been fed to the lysozyme, you
can watch them get eaten over time.
All in all a great choice for an intro lab.
Hope this helps.
--JK
Bruce Wightman wrote:
>> I was wondering if anyone could provide suggestions for good proteins to
> partially purify/purify for an introductory biochemistry lab for
> undergraduates. I'd like to be able to give students an experience with
> purification by gel filtration, ion exchange, and/or affinity
> chromatography. Preferably, the protein should have an activity that can
> be assayed easily and cheaply. The starting material should be something
> that's clearly recognizable as "life". Unfortunately, the number of steps
> has to be very limited, as time does not allow anything particularly
> complicated. Also, it has to be doable at room temperature.
>> Any ideas, good references, or protocols?
>> Thanks-
> Bruce Wightman
> Department of Molecular and Cell Biology
> 401 Barker Hall- Garriga Lab
> University of California
> Berkeley, CA 94720-3204
--
_____________
| ___/_
| |/ /
-- /\ // /--
|| || / /||
|| || / / ||
|| ||/ / ||
John Kuszewski || |/ /| ||
johnk at spasm.niddk.nih.gov || / /|| ||
\/ / / || \/
that's MISTER protein G to you! |/__/| |
/_________|
My parents went to Zaire and all I got
was this lousy retrovirus.