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Rabbit Reticulocyte Lysate/Wheat Germ Extract Combination System

The Rabbit Reticulocyte Lysate/Wheat Germ Extract Combination System contains both Rabbit Reticulocyte Lysate and Wheat Germ Extract for comparing in vitro translation systems. Reticulocyte Lysate is prepared from New Zealand white rabbits. The Wheat Germ Extract is prepared by grinding wheat germ in an extraction buffer followed by centrifugation to remove cellular debris. Both systems contain the cellular components necessary for protein synthesis. The systems have been treated with micrococcal nuclease, which destroys endogenous mRNA and results in minimal background translation.

The Rabbit Reticulocyte Lysate/Wheat Germ Extract Combination System contains both Rabbit Reticulocyte Lysate and Wheat Germ Extract for comparing in vitro translation systems. Reticulocyte Lysate is prepared from New Zealand white rabbits. The Wheat Germ Extract is prepared by grinding wheat germ in an extraction buffer followed by centrifugation to remove cellular debris. Both systems contain the cellular components necessary for protein synthesis. The systems have been treated with micrococcal nuclease, which destroys endogenous mRNA and results in minimal background translation.

Features - Benefits

Optimal Expression: Potassium Acetate is provided to enhance the Wheat Germ Extract System for a wide range of mRNAs.

Applications

For more information, see the Protein Expression chapter of the Protocols & Applications Guide.

Notes

Each system contains 2 × 200μl of both Rabbit Reticulocyte Lysate and Wheat Germ Extract; this is sufficient for 12 reactions using the Rabbit Reticulocyte Lysate and 12 reactions using the Wheat Germ Extract.

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