Nanostring

Nanostring

Nanostring Laboratory

The UAB Nanostring Laboratory in the Department of Radiation Oncology, directed by Eddy S. Yang, MD, PhD (eyang@uab.edu) and managed by Tiffiny Cooper, MS (tiffinyc@uab.edu), houses the nCounter Analysis System by Nanostring Technologies (Seattle, WA). This platform enables the profiling of hundreds of targets simultaneously with high sensitivity and precision, even in formalin fixed paraffin embedded (FFPE) tissues, without the need for amplification steps except in single cell or low cell number experiments. The system utilizes a novel digital technology that is based on direct multiplexed quantification of nucleic acids and provides highly reproducible data over 5 logs of dynamic range. Applications include gene expression analysis, microRNA and lncRNA analysis, copy number variation analysis, and ChIP-String analysis, and leukemia fusion gene analysis. Additionally, using the nCounter Elements Tagsets, users may assemble their own assays as part of development of a novel laboratory developed test.