Target details

Target details

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Alternative Name

TSC1/Hamartin

Background

TSC1 is a tumor-suppressor gene encoding a protein TSC1/Hamartin of about 130 kDa (ref. 1). Loss of its function causes TSC (tuberous sclerosis complex) or angiomyolipoma in human and in an animal model, TSC1 knockout mouse develops a renal tumor (ref. 2). TSC1/Hamartin, a product of TSC1, forms a complex with TSC2/Tuberin (ref. 3), a product of another tumor-suppressor gene TSC2 in cytoplasm (ref. 4). The complex suppresses mTOR activity and regulates cellular growth and division in a insulin signaling pathway (ref. 5). Under the condition of proliferation stimulus, forming of the complex is inhibited, and as a result, suppression to mTOR comes off and it leads to cellular growth and division.

Research Area

Cancer, Diabetes

Application Details

Application Details

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Application Notes

This antibody can be stained in formalin fixed paraffin embedded tissues by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) Method. The optimal dilution is 1 - 5 ?g /mL, however, the dilution rate should be optimized by each laboratories. This antibody can be used for western blotting in concentration of 1 - 5 ?g /mL.

References

References

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General

Kobayashi, Minowa, Sugitani et al.: "A germ-line Tsc1 mutation causes tumor development and embryonic lethality that are similar, but not identical to, those caused by Tsc2 mutation in mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 15, pp. 8762-7, 2001 (PubMed).