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Description

R&D Systems Recombinant Human IGF-I/IGF-1, CF

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I is synthesized as two precursor isoforms with N- and alternate C-terminal propeptides. These isoforms are differentially expressed by various tissues.

Reconstitutionof R&D IGF-1• It is recommended that sterile phosphate-buffered saline be added to the vial to prepare a working stock solution of no less than 100 μg/mL. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.

Storage of R&D IGF-1• Lyophilized samples are stable for up to twelve months at -20° C to -70° C. Storage • Upon reconstitution, this cytokine can be stored under sterile conditions at 2° - 8° C for one month or at -20° C to -70° C in a manual defrost freezer for three months without detectable loss of activity. • Avoid repeated freeze-thaw cycles.