FTIR and Raman spectra, resolution enhanced by second derivative and deconvolution techniques, have been used for studying protein secondary structure 1,2. A subtle conformational changes related to solvent denaturation and chemical modification can be distinguished. It has been shown that Raman spectroscopy combined with second derivative techniques and hydrogen-deuterium isotopic exchange can be successfully applied to identify he subtle comformational differences in creative kinase(EC 2.7.3.2)3.