This kit constitutes an all-in-one system for the rapid isolation of DNA from sputum samples. It allows for the isolation of bacterial or eukaryotic DNA from the sputum samples using a convenient spin column format. The kit includes all the reagents needed to process sputum DNA including a sputum liquifier to reduce viscosity and facilitate DNA isolation. The protocol can be completed in 30 minutes. Purified DNA is of the highest quality and free from inhibitors, and can be used in sensitive downstream applications including PCR and qPCR.

Background

A sputum specimen is the name given to the mucus which is expectorated from the lower airways. High quality sputum samples should contain very little saliva, as this may contaminate the sputum sample with oral bacteria. Sputum samples are typically evaluated to look for infections such as Moraxella catarrhalis,Mycobacterium tuberculosis, Streptococcus pneumoniae and Haemophilus influenza. Other pathogens can be detected in sputum as well, including HIV. In addition, sputum samples are useful in the detection of lung cancer or to evaluate chronic inflammation.

Kit Specifications

Time to Complete DNA Isolation

< 1 hour

Average Yield of DNA from 1.0 mL*

75 μg

Average Purity (OD260/280)

1.8 - 2.1

* Average DNA yield will vary depending on the health status of the donor

Storage Conditions and Product Stability
This kit contains ready-to-use Proteinase K which is dissolved in a specially prepared storage buffer. The Proteinase K should be stored at 4°C upon arrival (stable for 2 years) or at -20oC for long term storage. All other kit components should be kept tightly sealed and stored at room temperature (15 – 25 °C) for up to 3 years without any reduction in kit performance.