Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested.

Recommended Dilutions

ELISA: 1:8300

Western Blot: 1:500-1:2500

Immunohistochemistry: 1:200-1:1000

Optimal dilutions to be determined by the researcher.

Substrate

N2600-10 RT for 30 minutes.

Label

Alkaline phoshpatase (calf intestine) (MW 14kD)

Storage and Stability

May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only pror to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.