XL TLX3 BA

Break Apart Probe

XL TLX3 BA

Break Apart Probe

Description

XL TLX3 BA is designed as a break apart probe. The orange labeled probe hybridizes proximal to the breakpoint in the TLX3 gene region at 5q35, the green labeled probe hybridizes distal to the breakpoint.

Clinical Details

Acute lymphoblastic leukemia (ALL) is the most common childhood cancer type. T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive and quickly progressing type of ALL affecting T-lymphocytes. Genomic data suggests that more than 10 functional aberrations are contributing to the development of this disease. T-ALL cases can be grouped by distinct genetic profiles and the aberrant expression of a characteristic transcription factor. Major subgroups are characterized by ectopic expression of TAL1, TLX1, TLX3, HOXA9/10, LMO2 or NKX2-1 and others as a result of chromosomal rearrangements or mutations. About 20 % of childhood T-ALL cases are characterized by aberrant expression of TLX3 as a result of t(5;14)(q35;q32). This cryptic translocation juxtaposes TLX3, normally not expressed in T-cells, with the BCL11B gene which is active in T-cells and results in ectopic expression of TLX3. Fluorescence in situ hybridization is a valuable method for the detection of t(5;14)(q35;q32) since cryptic translocations may escape during classical cytogenetic analysis. Furthermore, the broad range of breakpoints in the chromosomal region 14q32 makes the development of efficient PCR-based methods difficult.

Clinical Applications

Acute Lymphoblastic Leukemia (ALL)

Images

XL TLX3 BA hybridized to lymphocytes. One normal interphase and metaphase are shown.

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MetaSystems Probes is proud to announce the new probes catalogue 2019/2020 with many new products, more information, and a brand new design. A PDF file of the new catalogue is available for download here on the download section of the MetaSystems Probes web site.

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