INTRODUCTION: The present study aimed to retrospectively analyze children with the diagnosis of neonatal cholestasis in terms of aetiology, diagnostic methods, laboratuvary findings as well as treatment modalities and long-term results.METHODS: Patients who had cholestasis in first three montshs of life included in this study. Patients were grouped as intrahepatic and extrahepatic cholestasis. Biliary atresia, inspissated bile syndrome and choledochal cysts were investigated in intrahepatic cholestasis group. Patients were evaluated retrospectively about their diagnosis, complaints, clinical courses, laborauary findings, responses to treatment and prognosis.RESULTS: Study group consisted of 43 female (%41), 62 male (%59), total 105 patients. 46 patients (%43) had premature delivery story. 36 patients (%34,3) were born between 30-37 gestation weeks, 10 patients (%9,5) were born before 30 gestation weeks and 39 patients were low birth weight infants. Intrahepatic cholestasis was determined in %81 of the patients; the remaining patients (%19) were extrahepatic cholestasis. With a total of 31 patients, acholic stools were detected in 18 (%90) of patients with extrahepatic cholestasis and in 13 (%15,3) of patients with intrahepatic cholestasis. Birth weightwas lower in patients with intrahepatic cholestasis than those with extrahepatic cholestasis. During the follow-up period, 38 (%44,7) patients with intrahepatic cholestasis and 7 (%35) patients with extrahepatic cholestasis had recovered.DISCUSSION AND CONCLUSION: Results of the present study have indicated a significant delay in the diagnosis of neonatal cholestasis. This delay can cause a decrease in the chance of early surgery in cases of BA. Differently from the literature, ultrasonographic examination showed no triangular cord sign in any patients, this may be because of a dearth of education and experience on this topic. Futhermore, underestimation of an increase in GGT, the presence of spleen, birth weight, and significant findings as acholic stools indicates the necessity to expand awareness of pediatric physicians.