Dr. Rosamma Philiphttps://dyuthi.cusat.ac.in:443/xmlui/handle/purl/2031
Thu, 22 Feb 2018 04:59:32 GMT2018-02-22T04:59:32ZIn vivo screening of mangrove plants for anti WSSV activity in Penaeus monodon, and evaluation of Ceriops tagal as a potential source of antiviral moleculeshttps://dyuthi.cusat.ac.in:443/xmlui/handle/purl/4440
In vivo screening of mangrove plants for anti WSSV activity in Penaeus monodon, and evaluation of Ceriops tagal as a potential source of antiviral molecules
Rosamma, Philip; Bright Singh, I S; Sudheer, N S
The objective of the study was to find out a natural way to fight white spot syndrome virus (WSSV) in cultured
shrimps, as the present scenario necessitated an organic remedy for the devastating pathogen in crustaceans.
Under this research programme seven mangrove plants were collected, identified and aqueous extracts
screened for their protective effect on the giant tiger shrimp Penaeus monodon against WSSV. The
experimental design consisted two modes of application, such as exposure of the virus to the extract and
injection challenge, and oral administration of the extract coated feed followed by oral challenge. All
experimental animals were monitored through a nested diagnostic PCR analysis. Of the seven mangrove
extracts screened aqueous extract from Ceriops tagal imparted total protection to shrimp from WSSV when
challenged by both methods. Shrimps administered with the aqueous extract from C. tagal were devoid of
virions. The HPLC fingerprint of the aqueous extracts from C. tagal showed more than 25 peaks and 7 of them
were larger and well separated. Preliminary phytochemical analysis revealed the presence of alkaloids,
flavonoids, polyphenolics, cardiac glycosides, saponins and sterols. The study indicated suitability of the
aqueous extract of C. tagal as a possible prophylaxis for WSSV infection in shrimp. This is the first report on the
anti WSSV property of the mangrove plant C. tagal
Aquaculture 311 (2011) 36–41
Tue, 23 Nov 2010 00:00:00 GMThttps://dyuthi.cusat.ac.in:443/xmlui/handle/purl/44402010-11-23T00:00:00ZSelection of Marine Yeasts for the Generation of Single Cell Protein from Prawn-shell Wastehttps://dyuthi.cusat.ac.in:443/xmlui/handle/purl/4439
Selection of Marine Yeasts for the Generation of Single Cell Protein from Prawn-shell Waste
Rosamma, Philip
Marine yeasts (33 strains) were isolated from the
coastal and offshore waters off Cochin. The isolates
were identified and then characterized for the utilization
of starch, gelatin, lipid, cellulose, urea, pectin,
lignin, chitin and prawn-shell waste. Most of the
isolates were Candida species. Based on the
biochemical characterization, four potential strains
were selected and their optimum pH and NaCI
concentration for growth were determined. These
strains were then inoculated into prawn-shell waste
and SCP (single cell protein) generation was noted in
terms of the increase in protein content of the final
product.
Bioresource Technology 65 (1998) 255-256
Thu, 01 Jan 1998 00:00:00 GMThttps://dyuthi.cusat.ac.in:443/xmlui/handle/purl/44391998-01-01T00:00:00ZAcute salinity stress alters the haemolymph metabolic profile of Penaeus monodon and reduces immunocompetence to white spot syndrome virus infectionhttps://dyuthi.cusat.ac.in:443/xmlui/handle/purl/4438
Acute salinity stress alters the haemolymph metabolic profile of Penaeus monodon and reduces immunocompetence to white spot syndrome virus infection
Rosamma, Philip; Annies, Joseph
Influence of acute salinity stress on the immunological and physiological response of Penaeus monodon to white spot
syndrome virus (WSSV) infection was analysed. P. monodon maintained at 15‰ were subjected to acute salinity changes to 0‰
and 35‰ in 7 h and then challenged orally with WSSV. Immune variables viz., total haemocyte count, phenol oxidase activity
(PO), nitroblue tetrazolium salt (NBT) reduction, alkaline phosphatase activity (ALP), acid phosphatase activity (ACP) and
metabolic variables viz., total protein, total carbohydrates, total free amino acids (TFAA), total lipids, glucose and cholesterol were
determined soon after salinity change and on post challenge days 2 (PCD2) and 5 (PCD5). Acute salinity change induced an
increase in metabolic variables in shrimps at 35‰ except TFAA. Immune variables reduced significantly (Pb0.05) in shrimps
subjected to salinity stress with the exception of ALP and PO at 35‰ and the reduction was found to be more at 0‰. Better
performance of metabolic and immune variables in general could be observed in shrimps maintained at 15‰ that showed
significantly higher post challenge survival following infection compared to those under salinity stress. Stress was found to be
higher in shrimps subjected to salinity change to lower level (0‰) than to higher level (35‰) as being evidenced by the better
immune response and survival at 35‰. THC (Pb0.001), ALP (Pb0.01) and PO (Pb0.05) that together explained a greater
percentage of variability in survival rate, could be proposed as the most potential health indicators in shrimp haemolymph. It can be
concluded from the study that acute salinity stress induces alterations in the haemolymph metabolic and immune variables of
P. monodon affecting the immunocompetence and increasing susceptibility to WSSV, particularly at low salinity stress conditions
Aquaculture 272 (2007) 87–97
Sat, 25 Aug 2007 00:00:00 GMThttps://dyuthi.cusat.ac.in:443/xmlui/handle/purl/44382007-08-25T00:00:00ZFenneropenaeus indicus is protected from white spot disease by oral administration of inactivated white spot syndrome virushttps://dyuthi.cusat.ac.in:443/xmlui/handle/purl/4437
Fenneropenaeus indicus is protected from white spot disease by oral administration of inactivated white spot syndrome virus
Rosamma, Philip; Bright Singh, I S; Manjusha, M; Somnath Pai, S
Fenneropenaeus indicus could be protected from white spot disease (WSD) caused by
white spot syndrome virus (WSSV) using a formalin-inactivated viral preparation (IVP) derived from
WSSV-infected shrimp tissue. The lowest test quantity of lyophilized IVP coated onto feed at 0.025 g–1
(dry weight) and administered at a rate of 0.035 g feed g–1 body weight d–1 for 7 consecutive days was
sufficient to provide protection from WSD for a short period (10 d after cessation of IVP administration).
Shrimp that survived challenges on the 5th and 10th days after cessation of IVP administration
survived repeated challenges although they were sometimes positive for the presence of WSSV
by a polymerase chain reaction (PCR) assay specific for WSSV. These results suggest that F. indicus
can be protected from WSD by simple oral administration of IVP
DISEASES OF AQUATIC ORGANISMS,Vol. 66: 265–270, 2005
Fri, 23 Sep 2005 00:00:00 GMThttps://dyuthi.cusat.ac.in:443/xmlui/handle/purl/44372005-09-23T00:00:00Z