Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.

NTIS

09/04/1991

Collation

13p

Abstract

The influence of enzyme induction on the acute toxicity of aniline and 4-chloroaniline to rainbow trout (Salmo gairdneri) was investigated. For these two xenobiotics, bioactivation reactions are known to occur in mammals. Induction of cytochrome P450 mixed-function oxidase was obtained by intraperitoneal (ip) injection of trout with a mixture of polychlorinated biphenyls (Aroclor 1254). Five days after ip injection with three different doses of Aroclor 1254 (50, 100, and 200 mg/kg), benzo(a)pyrene hydroxylase activity in trout liver microsomes increased five- to sixfold. Cytochrome P450 concentrations in the microsomes were slightly, but significantly, enhanced in two of the three dose levels. The 96-hr LC50's of aniline and 4-chloroaniline were not affected by pretreatment with Aroclor 1254, suggesting that metabolic activation does not necessarily play a role in the acute toxicity of aromatic amines to fish. (Copyright (c) 1990 Academic Press, Inc.)

Supplementary Notes

Pub. in Ecotoxicology and Environmental Safety, v20 p156-166 1990. Prepared in cooperation with Utrecht Rijksuniversiteit (Netherlands). Research Inst. of Toxicology.