It may not be possible to test for the presence of nitrous oxide in blood because it is eliminated from the blood through the lungs very quickly after use. Unless there is a particular reason to be looking for it, as in the case of an autopsy...it is extremely unlikely that most people will ever run into this test. It is not one of the SAMHSA-5 standardly tested for in the basic drug test, nor is it included in the extended drug tests. It is not chemically similar to any of the drugs tested for, so should not trigger the tests as another substance.

We have been told that the U.S. military does not test for Nitrous Oxide.

Erowid's drug testing information is a summary of data gathered from site visitors, journal articles, websites, and other resources. Detection periods depend on amount and frequency of use, metabolic rate, body mass, age, overall health, drug tolerance, and urine pH of each individual. Many substances that are not tested for in basic or extended urinalysis tests CAN be tested for by a laboratory willing to go to the trouble, although that is uncommon.