Technical Service

Frequently Asked Questions

Conjugate, substrate or wash buffer may be compromised. Washing may be excessive. Reagents may not have been brought up to room temperature prior to assay. Incorrect component or samples volumes may have been pipetted.

The microscope bulb maybe improperly aligned and/or the bulb maybe need to be replaced.
Components may not have been stored according to package insert.
Use humidity chamber for all incubation steps.
Improper mounting medium may auto-fluoresce. Phosphate-buffered glycerol (pH 7.2) mounting media must be used.