Current revision

Tips You (Mostly) Won't Find in the Manual

Use 1 colony in 2 mL of TB +antibiotic grown overnight.

TB gives you a higher plasmid yield than LB, but our lab currently doesn't make it (Janet 2/23/12). If your plasmid is high-copy number, one tube is sufficient. If you have a medium copy plasmid, grow 2-3 cultures from the same colony in parallel and pool them before loading the column.

Miscelaneous

Do not grow cultures with less than 4:1 air:liquid ratio -- it is very bad for plasmid prep. This is still one of Janet'sopen questions 2/23/12

You can elute in 30uL instead of 50uL to get a more concentrated sample.

Record the A260/A280 measurement down when you record the concentration. It should be between 1.8 and 2.

You can leave the cap on the fresh eppendorf tube you elute in. This allows you to pre-label the tube and stick it straight into the freezer after you are done instead of pipetting each into a fresh tube.