Author Address :

Abstract :

The aim of this study was to optimize cultural conditions required for in vitro seed germination, micropropagation with normal growth regulator medium and salt stress medium. Successfully 96% germination of seed was achieved in MS basal medium, and 92% in MS medium with NaCl 20 mM concentration. The bud explant cultured in MS medium augmented with BAP (11.10 µm) + KIN (1.16 µm) and NAA (0.98 µm) induced 91% shoots whereas the MS medium fortified with BAP (11.10 µm) + KIN (1.16 µm) + NAA (0.98 µm) and NaCl (20mM) produced 96% shoots. In the same medium the root formation occurs simultaneously along with shoots during subculture. The anatomical features of normal cells and tissues intact with normal size and shape of the cells. The shoots developed through in vitro showed presence of tannins and cells are enlarged deviating from normal spherical shape to spindle shape. Also less amount of cytoplasm and large number of vacuoles compared with filed grown. Secondary growth is very slower on salt induced experimental group when comparing with experimental and normal control. Further, vascular tissues is not fully regenerate in the experimental salt induced growth probably due to salt stress. Natural, in vitro and salt stress induced plant extracts showed the higher angiogenic activity 75.0 ± 1.42, 85.7 ± 1.24 and 80 ± 1.62 at the concentration 1000 µg/ml respectively.

Author Address :

Abstract :

The aim of this study was to optimize cultural conditions required for in vitro seed germination, micropropagation with normal growth regulator medium and salt stress medium. Successfully 96% germination of seed was achieved in MS basal medium, and 92% in MS medium with NaCl 20 mM concentration. The bud explant cultured in MS medium augmented with BAP (11.10 µm) + KIN (1.16 µm) and NAA (0.98 µm) induced 91% shoots whereas the MS medium fortified with BAP (11.10 µm) + KIN (1.16 µm) + NAA (0.98 µm) and NaCl (20mM) produced 96% shoots. In the same medium the root formation occurs simultaneously along with shoots during subculture. The anatomical features of normal cells and tissues intact with normal size and shape of the cells. The shoots developed through in vitro showed presence of tannins and cells are enlarged deviating from normal spherical shape to spindle shape. Also less amount of cytoplasm and large number of vacuoles compared with filed grown. Secondary growth is very slower on salt induced experimental group when comparing with experimental and normal control. Further, vascular tissues is not fully regenerate in the experimental salt induced growth probably due to salt stress. Natural, in vitro and salt stress induced plant extracts showed the higher angiogenic activity 75.0 ± 1.42, 85.7 ± 1.24 and 80 ± 1.62 at the concentration 1000 µg/ml respectively.