This study found that the ratio of Thiothrix eikelboomii to total bacterial concentrations (TH/TB) (%) was a better indicator of bulking incidents affecting effluent quality compared to absolute T. eikelboomii abundance alone. This was determined using a genus-specific Thiothrix quantitative PCR primer and probe set, which was developed in this study to… (More)

SUMMARY In this paper, we propose a hybrid fine-tuned multi-objective memetic algorithm hybridizing different solution fitness evaluation methods for global exploitation and exploration. To search across all regions in objective space, the algorithm uses a widely diversified set of weights at each generation, and employs a simulated annealing to optimize… (More)