Fig. 1

Methylation profile of DNA in the promoter of the MTHFR gene in patients with diabetes with and without complications. a Representative samples of the groups studied after MSP (180 bp). MW molecular weight, M methylated, U unmethylated. b Methylation frequency of Control (n = 60), DR (n = 16) and DN (n = 29) (*p < 0.01; **p < 0.03; Χ2). Partial methylation was defined as when the sample was positive for both methylated and unmethylated conditions (MSP)