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A stacked insect-resistant and herbicide-tolerant maize derived
from conventional cross-breeding of BT11 x DAS-59122 x TC1507 x
GA21. Insect resistance is conferred by the cry1A(b), cryIF,
cry34Ab1, and cry35Ab1 genes from the common soil bacterium
Bacillus thuringiensis, and glufosinate and glyphosate herbicide
tolerance through the pat and EPSPS genes respectively.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

DNA insert from GA21 vector pDPG434
The 5-enolpyruvyl shikimate-3-phosphate synthase (epsps) gene from
maize was modified through site-directed mutagenesis, such that its
encoded enzyme was insensitive to inactivation by glyphosate. The
resulting gene was used to develop GA21

DNA insert from 59122 vector PHP17662
The cry34Ab1 and cry35Ab1 genes, isolated from the common soil
bacterium Bacillus thuringiensis (Bt) strain PS149B1, produce the
insect control proteins (delta-endotoxins) Cry34Ab1 and Cry35Ab1.
The pat gene was isolated from the soil bacterium Streptomyces
viridochromogenes and confers tolerance to herbicides containing
glufosinate ammonium.

DNA insert from TC1507 vector PHI8999A
TC1507 modified with the insertion of the Cry1F geneto confer
resistance to the European corn borer (Ostrinia nubilalis). A
transformation cassette coding for phosphinothricin (PPT) herbicide
tolerance, specifically glufosinate ammonium, was also inserted
into the organism.

For additional information on this LMO, please refer to the records
of the parental LMOs.