Re: [Histonet] (no subject)

From:

Lori Richey

We fix all precut control tissue slides in cold acetone for 5
minutes,and allow them to air dry before storing them in the -70.
Matt Prideaux wrote:
>Dear Histonetters,
>
>A colleague wishes to store cytospun cell preparations in the freezer before
>doing immunocytochemistry for CD31 and CD44. She would like to know whether
>it's better to fix the slides before freezing or after thawing them prior to
>staining. Also, which fixatives would you recommend?
>
>Matt
>
>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mai