Ab00455-1.1 Anti-EGFR [L3 11D]

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse scFv antibody fragment format, for improved compatibility with existing reagents, assays and techniques.

Species and Isotype: Mouse IgG1, kappaClone Number: L3 11DUniProt Accession Number of Target Protein: Q01279Alternative Name(s) of Target: Epidermal growth factor receptor; ERBB; ERBB1; HER1; mENA; PIG61; NISBD2Published Application(s): ELISA; WB; SDS-PAGE; FCPublished Species Reactivity: MouseImmunogen: Mouse EGFR.Specificity: The antibody binds to mouse EGFR with an affinity (Kd) of 0.21 nM.Application Notes: The antibody binds specifically to EGFR, a cell-surface receptor which is part of the ErbB family of receptors. The EGFR is a receptor tyrosine kinase, which, upon activation by its ligands, such as epidermal growth factor and transforming growth factor alpha, triggers signal transduction pathways that increase cell migration, adhesion and proliferation. Mutations that lead to the overexpression of EGFR are associated with the development of cancer. The antibody was prepared from phage antibodies isolated from the lymph node library.Antibody First Published in: Kettleborough et al. Isolation of tumor cell-specific single-chain Fv from immunized mice using phage-antibody libraries and the re-construction of whole antibodies from these antibody fragments. Eur. J. Immunol. 24 (4), 952-958 (1994) PMID:8149964Note on publication: Describes the phage-antibody technology, which is used to produce three phage-antibody libraries from which mouse anti-EGFR scFv antibodies are generated. The libraries are prepared from the spleen, lymph node and in vitro cells of an immunized mouse. The antibodies produced are compared with a mouse mAb, 425, isolated by standard hybridoma technology.

Mouse IgG1

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Product Form

Purification: Protein A affinity purifiedSupplied in: PBS with preservative (0.02% Proclin 300)Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°CConcentration: See vial label.

Important note – This product is for research use only. It is not intended for use in therapeutic or diagnostic
procedures for humans or animals.