Cytokine Arrays

To determine multiple cytokines in a single sample at a single time point, Randox offers a range of unique cytokines arrays allowing complimentary cytokines to be analysed within the same sample.

The combination of highly specific antibodies and advanced chemistries enables cytokines, cytokine receptors and growth factors to be detected simultaneously in a single sample, providing valuable information relating to each cytokine under test and possible associations between cytokines in each sample.

Cytokine Array I

Analyte

Intra-Assay Precision (n=20)

Intra-Assay Precision (n=20)

Level 1 %CV

Level 2 %CV

Level 3 %CV

Level 1 %CV

Level 2 %CV

Level 3 %CV

IL-Ia

9.7

7.7

6.8

10.7

5.9

7.3

IL-1b

8.2

6.3

6.7

13.1

7.3

7.0

IL-2

9.6

5.0

6.3

10.0

7.2

7.6

IL-4

10.7

9.4

4.6

10.0

6.4

7.8

IL-6

12.9

6.1

8.7

15.4

10.4

8.7

IL-8

10.1

7.5

7.9

10.1

8.7

9.9

IL-10

6.3

5.5

6.7

9.4

6.3

6.1

VEGF

7.3

4.6

4.3

13.4

7.6

6.0

IFNγ

12.7

6.2

3.8

13.4

9.8

9.1

EGF

4.6

5.8

7.6

7.4

4.0

7.2

MCP-I

6.8

5.1

4.7

14.7

6.0

5.6

TNFI

10.2

6.0

7.8

13.0

8.1

11.6

Analyte

Calibration Range pg/ml

Sensitivity pg/ml

IL-Ia

0 - 500

0.8

IL-Ib

0 - 250

1.6

IL-2

0 - 3000

4.8

IL-4

0 - 900

6.6

IL-6

0 - 900

1.2

IL-8

0 - 3000

4.9

IL-10

0 - 1000

1.8

VEGF

0 - 3000

14.6

IFNy

0 - 1500

3.5

EGF

0 - 900

2.9

MCP-I

0 - 1500

13.2

TNFa

0 - 1500

4.4

Accuracy and Precision based on Evidence Investigator

Assay range may vary with batch of calibrators.
Inter-assay precision was determined by assaying 2 replicates of each of three levels of sample in 10 separate assays.
Intra-assay precision was determined by assaying 20 replicates of each of three levels of sample.

Cytokine Array II

Performance data coming soon

Cytokine High Sensitivity Array

Analyte

Intra-Assay Precision (n=20)

Intra-Assay Precision (n=20)

Level 1 %CV

Level 2 %CV

Level 3 %CV

Level 1 %CV

Level 2 %CV

Level 3 %CV

IL-Ia

10.9

9.7

11.4

15.5

8.9

13.1

IL-1b

9.3

8.7

7.4

9.9

11.8

8.7

IL-2

7.8

5.8

6.9

7.9

6.5

8.2

IL-4

8.1

9.5

8.4

11.8

8.6

8.8

IL-6

11.9

9.8

7.8

8.4

7.4

8.4

IL-8

9.4

9.4

7.0

11.1

9.9

9.2

IL-10

6.8

5.6

6.1

6.7

6.5

7.5

VEGF

10.4

10.8

7.3

12.0

7.2

10.7

IFNy

10.1

7.4

7.7

11.4

6.4

7.6

EGF

9.2

9.5

9.6

11.7

8.5

9.6

MCP-I

12.2

8.9

5.8

12.8

10.4

7.2

TNFI

7.1

7.2

12.7

8.6

6.7

7.0

Analyte

Calibration Range pg/ml

Sensitivity pg/ml

IL-Ia

0 - 225

0.19

IL-Ib

0 - 112.5

0.26

IL-2

0 - 1200

2.97

IL-4

0 - 450

2.12

IL-6

0 - 400

0.12

IL-8

0 - 1450

0.36

IL-10

0 - 450

0.37

VEGF

0 - 1000

3.24

IFNY

0 - 600

0.44

EGF

0 - 450

1.04

MCP-I

0 - 500

3.53

TNFa

0 - 600

0.59

Accuracy and Precision based on Evidence Investigator

Assay range may vary with batch of calibrators.
Inter-assay precision was determined by assaying 2 replicates of each of three levels of sample in 10 separate assays.
Intra-assay precision was determined by assaying 20 replicates of each of three levels of sample.

Cytokine Array III

Analyte

Intra-Assay Precision (n=20)

Intra-Assay Precision (n=20)

Level 1 %CV

Level 2 %CV

Level 3 %CV

Level 1 %CV

Level 2 %CV

Level 3 %CV

IL-5

5.1

8.8

6.5

10.3

9.3

8.1

IL-I5

6.3

8.2

6.2

8.4

6.5

8.6

GMCSF

6.8

6.4

5.2

9.8

9.7

9.7

MIP-1a

9.5

7.8

8.4

11.8

8.6

8.8

Analyte

Calibration Range pg/ml

Sensitivity pg/ml

IL-5

0 - 1000

0.98

IL-I5

0 - 500

0.74

GMCSF

0 - 500

1.46

MIP-1α

0 - 1500

4.98

Accuracy and Precision based on Evidence Investigator

Assay range may vary with batch of calibrators.
Inter-assay precision was determined by assaying 2 replicates of each of three levels of sample in 10 separate assays.
Intra-assay precision was determined by assaying 20 replicates of each of three levels of sample.

Cytokine Array IV

Analyte

Intra-Assay Precision (n=20)

Intra-Assay Precision (n=20)

Level 1 %CV

Level 2 %CV

Level 3 %CV

Level 1 %CV

Level 2 %CV

Level 3 %CV

sIL-2Rα

7.2

6.6

7.8

6.2

8.7

10.1

sIL-6R

6.7

5.0

7.4

6.1

7.1

8.4

sTNFR I

8.4

8.3

13.9

9.5

12.2

13.1

sTNFR II

9.3

9.2

10.2

9.4

10.3

9.3

MMP-9

10.1

8.7

5.3

8.9

10.2

7.9

Analyte

Calibration Range ng/ml

Sensitivity ng/ml

sIL-2Rα

0 - 40

0.12

sIL-6R

0 - 400

0.62

GsTNFR I

0 - 25

0.09

sTNFR II

0 - 75

0.20

MMP-9

0 - 1000

3.03

Accuracy and Precision based on Evidence Investigator

Assay range may vary with batch of calibrators.
Inter-assay precision was determined by assaying 2 replicates of each of three levels of sample in 10 separate assays.
Intra-assay precision was determined by assaying 20 replicates of each of three levels of sample.

Cytokine Array V

Analyte

Intra-Assay Precision (n=20)

Intra-Assay Precision (n=20)

Level 1 %CV

Level 2 %CV

Level 3 %CV

Level 1 %CV

Level 2 %CV

Level 3 %CV

IL-3

6.8

6.8

7.8

7.3

10.2

10.7

IL-7

6.3

7.5

8.0

8.7

10.0

9.0

IL-12p70

8.2

6.8

10.0

4.9

7.5

8.2

IL-13

5.5

6.0

8.3

5.9

8.0

10.6

IL-23

7.3

7.2

5.1

6.6

7.3

4.6

Analyte

Calibration Range ng/ml

Sensitivity ng/ml

IL-3

0 - 2,000

8.78

IL-7

0 - 200

1.11

IL-12p70

0 - 1,000

2.61

IL-13

0 - 2,000

5.23

IL-23

0 - 10,000

13

Accuracy and Precision based on Evidence Investigator

Assay range may vary with batch of calibrators.
Inter-assay precision was determined by assaying 2 replicates of each of three levels of sample in 10 separate assays.
Intra-assay precision was determined by assaying 20 replicates of each of three levels of sample.