Federal Liberal Member for McMillan Russell Broadbent and Anglican Bishop of Gippsland John McIntyre have both support the retention of the prayer amid a campaign pushing for it to be scrapped.

Greens Senator Richard di Natale last month called for an end to the prayer being read because it was not representative of people of all faiths.

He said he would pursue the issue with MPs when Federal Parliament resumed.

Parliamentary sittings began this week - and the Lord's Prayer continues to be read at the start of the day.

"It's a magnificent tradition of the parliament and the history of our Parliament. This prayer was first read and promoted by the Presbyterian Church of NSW on the 14th June, 1901," Mr Broadbent said.

"I think that's a magnificent way to start the day, seeking God's blessing upon the Parliament because if any institution needs God's blessing, it's the Federal Parliament of Australia."

Mr Broadbent said some MPs stayed outside Parliament while the Lord's Prayer was being read but returned for the acknowledgement of country.

"That's up to them - that's fine," he said.

Bishop John McIntyre disagreed that the lines separating church and state in Australia were blurred because the prayer is recited.

"The principle behind separation of church and state is to ensure that the institution of church does not meddle in the affairs of parliament," he said.

"It's about an institutional separation of power. It's not about acknowledging that there are values that often emerge within the context of religious belief to which we would happily commit - issues like justice and mercy for example."

The Lord's Prayer is also read before municipal council meetings in the Gippsland region.

The Anglican Bishop for Gippsland John McIntyre and Liberal Member for McMillan Russell Broadbent discuss a push by the Greens to stop the Lord's Prayer from being read at the beginning of each Parliamentary sitting day.