FYB (FYN binding protein) was initially identified as a molecular adaptor that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of FYB, ultimately activates the NFkB family of transcription factors. More recent experiments have shown that FYB controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bclx(L), suggesting that FYB regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of FYB are known to exist.

Catalog #

A0859-43

Applications

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions

Western Blot: 0.5-2.0ug/ml detected ADAP/SLAP-130/Fyb in RIPA lysates from RAW 264.7 macrophage cells. RAW 264.7 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A0859-43. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Clone Type

Polyclonal

Isotype

IgG

Host

Rabbit

Source

Mouse

Concentration

~1mg/ml

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.