RE: [Histonet] Diastase dilemma

From:

"Charles.Embrey"

Did you ever stop to think.... that if the patient tissue were positive for
Glycogen then the digestion would remove it and it would show no PAS
staining? The positive reaction on could be mucin or acid mucosubstances.
Charles Embrey
-----Original Message-----
From: Jackie.O'Connor@abbott.com [mailto:Jackie.O'Connor@abbott.com]
Sent: Friday, August 13, 2004 2:31 PM
To: Angela Bitting
Cc: histonet@lists.utsouthwestern.edu;
histonet-bounces@lists.utsouthwestern.edu
Subject: Re: [Histonet] Diastase dilemma
Did you stop to think that your patient just might be positive for
glycogen?
"Angela Bitting"
Sent by: histonet-bounces@lists.utsouthwestern.edu
08/13/2004 02:05 PM
To:
cc:
Subject: [Histonet] Diastase dilemma
Bizarre things happen here all the time, but this is REALLY BIZARRE!
We'll do a PAS w/ diastase and our control tissue will be digested but the
patient tissue won't be. They are mounted on the same slide!
The next day we will run the stain again and we tried turning the slide
upside-down in the Coplin jar. Same problem.
We mixed fresh diastase solution and tried again. Same problem.
Tomorrow, we may run it again and it will work fine.
What could be going on?
I'm perplexed!
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave.
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
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