BYOSENS LYTE96

THE FIRST PORTABLE LABEL-FREE

MICROPLATE READER

The Lyte96 is the first portable label-free reader. Based on the Corning® Epic® System it is designed to read out 96-well microplates and to perform a wide range of cellular assays. In combination with a wireless connection and an integrated battery, the compact construction of the Lyte96 makes it entirely mobile and easy to integrate in liquid-handling-systems.

The next generation label-free reader

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Mobility: The innovative design of the Lyte96 gives the customer the benefit of total flexibility. The compact construction in combination with a wireless connection and an integrated battery makes the Lyte96 entirely mobile. This makes it the perfect analytic tool for researchers and drug developer who want to explore new possibilities.

Usability: The Lyte96 simplifies the research and development process in the laboratory. Experiments can be started without complex presettings and the intuitive complementary software guarantees a high level of usability. Thanks to the patented technological system, the Lyte96 is almost free of maintenance requirements.

Data Analysis: Based on the established Corning® Epic® System the high sensitivity of the Lyte96 enables to conduct a broad spectrum of cellular assays. Real time data is provided from the very beginning of the experiment up to several days for long time studies.

Corning® Epic® System

The Lyte96 is designed to read out Corning® Epic® 96-well cellular assay microplates. These microplates are ANSI / SBS standard 96-well microplates with patentedoptical biosensor integrated into each well.

The standard uncoated cellular microplate is tissue culture compatible and enables the attachment and normal growth of adherent cells, including nativecells, recombinant/engineered cell lines, and primary cells. The surface of each Epic® fibronectin-coated cellular assay microplate enables the attachment and the growth of weakly adherent cells.

LABEL-FREE DETECTION

Label-based techniques have been used for revealing biological interactions for a long time. But there are issues that impact the bioactivity of the labeled biological system. Also the incompatibility with living cells is a disadvantage of label-based techniques. In comparison, label-free detection is the more native, simpler and more physiological alternative. It delivers real-time data on biological process, which is especially important for living cell assays.

Main features of label-free

Live-cell response in real time

More native environment

No cellular manipulations (non-invasive)

Simplified assays

TECHNOLOGY

The technology of the Lyte96 label-free reader is based on a patented optical reflection measurement. For every well a separate detection unit consisting of light emitting diodes (LEDs), photodiodes and a sophisticated optic is implemented. These detection units use the intensity of the reflection to measure even smallest mass changes on the surface of the Epic® biosensor. The detectable area is defined by the penetration depth of the light out of the sensor, which is about 200 nm.

1. Surface coating

2. Gratting sensor

3. Detectable area ~200nm

Cell

Incident light

Reflected light

Cellular Assays

With the Lyte96 label-free reader complex cellular mechanisms both of endogen and of recombinant target molecules can be characterized and analyzed. The origin of the signal can be either a cellular mass change or a dynamic mass redistribution (DMR). Experiments can be observed from the very beginning of the experiment up to several days.

Cell colony

Add compound

Mass change

Examples of mass change assays

Adhesion / Spreading

Proliferation

Apoptosis / Cytotoxicity

Dynamic mass redistribution (DMR)

Examples of DMR assays

Signal transduction pathways (e.g. GPCR)

Receptor tyrosine kinases (e.g. EGF)

Case Study

Mass change assay

In a proliferation assay the number of cells is proportional to the mass on the sensor surface and is monitored in real time with the Lyte96. The microplate and the Lyte96 can be placed in the incubator with a wireless connation to the computer via Bluetooth. In the exemplary proliferation assay A431 cells were added to the wells and cell growth is recorded at 37˚C.

Dynamic mass redistribution(DMR) assay

Like in many other signaling assays, during a GPCR assay dynamic mass redistribution (DMR) is detected by the Lyte96 Label-free Reader. In this example a bradykinin assay with A431 cells is performed at room temperature. The obtained EC50 was 0.45 nM, which is similar to results from the literature.