Advanced Cardiomyocyte Cell Culture

Discovery, Regenerative Medicine, Toxicity

Advanced cell culture techniques including 3D spheroids, micropatterned co-culture, bioengineered and flow-based systems, and bioprinting offer the potential to better mimic in vivo tissue structure and function. CDI’s cardiomyocytes are amenable to these culture techniques as pure cell populations or in co-culture with other cell types, such as CDI’s iCell Endothelial Cells.

Bioengineering Cardiac Tissues

Regenerative Medicine

CDI’s cardiomyocytes are being applied in research aimed to develop and test a bioengineered, implantable cardiac patch for the treatment of heart failure. Initial studies demonstrate that the cardiac patches beat spontaneously and synchronously, respond to electrical stimulation, exhibit typical morphology, and improve cardiac function in rats with chronic heart failure.

Advanced Neural Cell Culture

Discovery, Regenerative Medicine, Toxicity

Advanced cell culture techniques including 3D spheroids, micropatterned co-culture, bioengineered and flow-based systems, and bioprinting offer the potential to better mimic in vivo tissue structure and function. CDI’s neurons are amenable to these culture techniques as pure cell populations or in co-culture with other cell types, such as CDI’s astrocytes.

Genetic Manipulation of Neurons

Discovery, Regenerative Medicine, Toxicity

The ability to interrogate and monitor gene expression is critical to understanding biological pathways that underlie normal and pathogenic cellular function. CDI’s neurons and dopaneurons are amenable to various genetic manipulation techniques including:

Measuring Cardiac Progenitor Cell Proliferation and Differentiation

Discovery, Regenerative Medicine, Toxicity

The ability to model human cell and organ developmental pathways is critical to understanding developmental toxicities and disease pathways and to developing therapeutic strategies for tissue regeneration. CDI’s cardiac progenitor cells are multipotent cardiomyocyte precursor cells that exhibit robust and measurable proliferation and differentiation. Assays with these cells are being used in targeted and phenotypic screens to identify therapeutic candidates for cardiac regeneration.

Genetic Manipulation of Hepatocytes

Discovery, Regenerative Medicine, Toxicity

The ability to interrogate and monitor gene expression is critical to understanding biological pathways that underlie normal and pathogenic cellular function. CDI has evaluated various genetic manipulation tools to enable the development of assays using its hepatocytes.

Vascular Tissue Bioengineering

Discovery, Regenerative Medicine, Toxicity

Vascular networks supply organs with oxygen and nutrients, remove waste, and serve generally as the delivery network within the body. Thus, any bio- or tissue engineering effort should include a vascular framework to support organ function. CDI’s endothelial cells have demonstrated functionality to reform vascular networks in decellularized organs to support de novo organ synthesis as a transplantable tissue for regenerative medicine approaches. In addition, CDI’s endothelial cells have formed complex vascular networks in static- and flow-based bioengineered vascular platforms.

Measuring Vasculogenesis

Discovery, Regenerative Medicine

The ability to modulate vasculogenesis has utility in tissue engineering and repair as well as in oncology therapeutics development aimed at targeting angiogenesis. The processes of endothelial cell migration and invasion and vascular sprouting behavior can be measured using CDI’s endothelial cells using platforms including:

Measuring Neuronal Electrophysiology

Discovery, Regenerative Medicine, Toxicity

The communication between neurons and between neurons and other cell types is accomplished through electrical signals. CDI’s neurons exhibit biologically relevant electrical functions typical of primary human cortical neurons including evoked and spontaneous action potentials, inhibitory and excitatory post-synaptic currents, and ion channel pharmacology. These responses can be measured using platforms including:

Measuring Vascular Endothelial Cell Proliferation

Discovery, Regenerative Medicine, Toxicity

The regulation of endothelial cell proliferation plays a fundamental role in vascular remodeling and angiogenesis in normal and pathological conditions. CDI’s endothelial cells exhibit a dose-dependent proliferation response to VEGF that is sensitive to inhibition by tyrphostin, a selective VEGF receptor inhibitor, as measured using the CellTiter-Glo Assay (Promega).

Genetic Manipulation of Endothelial Cells

Discovery, Regenerative Medicine, Toxicity

The ability to interrogate and monitor gene expression is critical to understanding biological pathways that underlie normal and pathogenic cellular function. CDI has worked to evaluate a wide range of genetic manipulation tools to enable the development of assays using its endothelial cells.

3D Spheroid Cell Culture

Discovery, Regenerative Medicine, Toxicity

Moving beyond traditional static-plated culture yields a more liver-like environment for hepatocyte assays and generates more predictive biology in vitro. The combination of iPSC technology with advanced culture techniques offers advantages over existing models. Conditions developed allow for a tunable spheroid size with maintenance of viability and put the control over engineered tissue in the hands of the user. This novel workflow allows for the generation of iCell® Hepatocytes 2.0 microtissues in low-attachment plates.

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