Characterization

Description:

The Wnt signaling pathway is important to both embryonic development and tumorigenesis. β-Catenin, the central component of the pathway, functions as a cofactor for a number of transcription factors. These transcription factors then activate transcription of Wnt target genes involved in cell proliferation, survival, and migration. Characterization of the activation of TF involved in the Wnt/ β-Catenin pathway can lead to greater understanding of various disease states and the development of treatments.

Principle

Signosis’ TF Activation Profiling Arrays are used for monitoring the activities of multiple TFs simultaneously. In the technology, a series of biotin-labeled probes are made based on the consensus sequences of TF DNA-binding sites. When the probe mixtures incubate with nuclear extracts, individual probes will find their corresponding TFs and form TF/probe complexes, which can be easily separated from free probes through a simple spin column purification. The bound probes are then detached from the complexes and analyzed through hybridization with a plate, which each well is specifically pre-coated with complementary sequences of the probes.