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Tear Mucin Assay Kit (O-Glycan Assay Method)

Cat. No.

CSR-MUC01E

Quantity

1KIT
（50TEST）

Price

¥
96,000$ 1280€ 960

Inventory

Available

Other

Mucins are major components in tear fluid and apical cell membranes on the ocular surface epithelia. Structurally, they are composed of tandem repeat domains containing heavily O-glycosylated serine and threonine residues. More than a half of its weight consists of O-glycans, which has hydrophilic nature. The heavy glycosylation of mucins is believed to impart a highly negative charge and a hydrophilicity that provides a barrier to pathogen adherence and penetrance into the epithelium. Alteration in both secreted and membrane-associated mucins occur in drying ocular surface diseases. At the ocular surface, three types of mucins are present. The large gel-forming mucin MUC5AC is expressed by conjunctival goblet cells. Some cells of the lacrimal gland acini express the small soluble mucin MUC7. The corneal and conjunctival epithelia express the membrane-associated mucins MUCs 1, 4, and 16.
Mucins are family of high molecular (1000 kda-10000 kda) and heavily glycosylated protein. Mucin domains within the protein core are rich in threonine and serine. The reducing ends of sugar chain N-acetylgalactosamin (GalNAc) are linked to those amino acids by the post-translational O-glicosylation. Mucin content can be measured as reducing ends of sugar chain afterβ-eliminated by diluted alkali. Reducing ends of sugar chain react at high temperatures with 2-cyanoacetamide (2-CAN) to produce intensely fluorescent condensate.