The conditional KO allele has been obtained after Flp-mediated removal of the selection marker by crossing chimeras to a Flp-Deleter on a C57BL/6 background.

The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.

Deletion of exon 9 should result in the loss of function of the Sykbgene by deleting the ATP-binding domain and part of the kinase domain and by generating a frameshift from exon 9 to all downstream exons (premature Stop codon in exon 10).

The remaining recombination sites will be located in non-conserved regions of the genome.