Genomic DNA (the QIAamp DNA Mini Kit, QIAamp DNA FFPE Tissue Kit, or GeneRead DNA FFPE Kit are recommended for extraction) can be quantified and qualified using the GeneRead DNA QuantiMIZE System. The GeneRead DNAseq Targeted Panels V2 are then used for targeted enrichment. Following target enrichment, construct your NGS library, quantify and quality-control using the GeneRead Library Quantification System, perform NGS, and analyze the data using the QIAGEN NGS Data Analysis Web Portal.|The principle of the GeneRead DNAseq V2 System is to employ overlapping primer sets across the exonic portions of a gene or genes to maximize target coverage and minimize nonspecific amplification.|

The performance of GeneRead DNAseq Custom Panels V2 is assessed by three criteria: design coverage, specificity, and coverage uniformity. Greater than 90% of exonic regions are covered by the panel primer design, enhancing potential variant discovery. The high specificity of the system maximizes efficient use of sequencing capacity, as more than 90% of sequencing reads align to target regions. Finally, the high coverage uniformity of the system, with more than 90% of targeted bases covered at >20% median sequencing depth, ensures high-quality variant calls.

Principle

The GeneRead DNAseq Custom Panels V2 employ overlapping primer sets across the exonic portions of a gene or group of genes to maximize target coverage (see figure Multiplex PCR-enabled target enrichment of genomic regions of interest). Overlapping primer sets are divided into 4 pools, thereby increasing the specificity of coverage without primer dimers and nonspecific amplifications. Following amplification and purification, enriched regions from each sample are pooled together, yielding one library preparation for each sample.

Virtual panels are custom panels created from gene lists compiled by bioinformatics experts for specific diseases not covered by the GeneRead DNAseq Targeted Panels V2 catalog. For a complete list, scroll down to Applications.

Note: The GeneRead DNAseq Custom Panel Builder is under construction. To build your custom panel, please email your design request to BRC.Custom@qiagen.com, and one of our scientists will follow up with you.

Applications

GeneRead DNAseq Custom Panels V2 are highly suited for enriching a panel of any genomic regions of interest in the human genome to determine genetic variants through NGS analysis. Virtual panels are highly suited for enriching a panel of recommended genomic regions of interest for particular diseases and pathways.