LIS Resources

Appropriate specimens are inoculated onto blood agar and into thioglycollate broth which are incubated under anaerobic conditions. Cultures are examined after 48 hours of incubation and thereafter (maximum of 14 days) for the presence of organisms that have characteristic colonial and Gram-stain morphologies. Definitive identification is made using 16S rRNA sequencing or a variety of biochemical tests and gas-liquid chromatographic analysis of volatile fatty acids that are produced by fermentation of glucose.(Koneman EW, Allen SD, Janda WM, et al: Color Atlas and Textbook of Diagnostic Microbiology. Fifth edition. New York, Lippincott-Raven Publishers, 1997, pp 709-784)