Pyrimidine derivatives have been in use since ages. Pyrimidine derivatives have shown wide range of pharmacological actions. However still many activities haven’t been researched so far. It is because of this reason the derivatives of the pyrimidine compounds was selected for the present study. In the present research work, it is all about the newer compounds being developed, designed, and experimented for their pharmacological actions. The current research shows the antibacterial activity tested in the new compounds designed in the laboratory. The antimicrobial activities were tested using agar plate and well diffusion method was especially selected for the present research work. Complete and proper steps were followed as per the guidelines. All precautions were taken while carrying out research for the derivative compounds in the present study. The designed compound showed effective and positive antibacterial activity present in the compound designed in the laboratory. Activity on both Gram positive and Gram negative microbes was tested, and the outcomes were in affirmation of the derivative compounds to be studied further for their pharmacological activities on both Gram positive and Gram negative microbes, and the outcomes were in affirmation of the derivative compounds to be studied further for their pharmacological activities for the derivative compounds selected in the present study. As a researcher, it is recommended to carry out further research on the same compound and being tested for other pharmacological activities.