This assay measures alpha- (the main source of activity) and combined beta- and gamma-tocopherols. The bioavailability of Vitamin E may be impaired in hyperlipidemic states, in which it may be useful to simultaneously measure Cholesterol, Total or Lipids, Total. The Vitamin E/lipid ratios calculated as (100 x Vitamin E [in mg/L])/(lipid [in mg/dL]) are normally > 3 mg/g for E/cholesterol or > 1 mg/g for E/total lipids.