Culture of Endometrial Explants
(P.J. Hansen Lab)This method is used to study secretion of proteins and prostaglandins by endometrium from the cow, ewe, mare, bitch and other species. The technique is also useful for culture of peri-implantation conceptuses and placental tissues for metabolic labelling studies and to obtain conceptus secretory proteins for biological studies.http://www.animal.ufl.edu/hansen/protocols/endocul...Added: Tue May 14 2002, Hits: 1147, Reviews: 0Write reviewCached

Direct tissue Explant for Derivation and Study of Extravillous Trophoblast
(Aplin Laboratory, Reproductive Biology at the University of Manchester )This method enables the researcher to explant terminal placental villi onto a three dimensional matrix of either collagen or Matrigel and monitor the formation of anchoring cytotrophoblast columns. The resulting cells can be stimulated to assume a migratory phenotype by growth factors. TGFb1 inhibits outgrowth.http://www.medicine.man.ac.uk/aplin/protocols1.htm...Added: Fri Dec 13 2002, Hits: 925, Reviews: 0Write reviewCached

Staining Protocols for Explant Cultures
(Aplin Laboratory, Reproductive Biology at the University of Manchester )The protocol uses a single primary antibody with nuclear counterstaining, but it can be extended to double antibody staining. It requires an inverted microscope with fluorescence illumination and long working distance objectives.http://www.medicine.man.ac.uk/aplin/protocols2.htm...Added: Fri Dec 13 2002, Hits: 1922, Reviews: 0Write reviewCached