Inoculation of ''Arabidopsis thaliana'' with Microbes

True leaves of young Arabidopsis thaliana can be inoculated via either a foliar spray [1] or pipetting [2] of the microbial suspension. A foliar spray would likely coat the leaves more evenly and effectively, but it would also likely inoculate the agar as well, potentially leading to adverse bacterial growths on the agar surface. Pipetting would be more labor intensive and possibly less effective at leaf inoculation, but it would ensure that only the plant received the microbial suspension.