Target

FunctionThis is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.

Predicted band size : 47 kDaWestern blot analysis of 5HT1B Receptor in human brain lysate with ab13896. A protein band of approximate molecular weight of 40-41kDa was detected. Western blot analysis of 5HT1B Receptor in human brain lysate with ab13896. A protein band of approximate molecular weight of 40-41kDa was detected.

ab2607 (4µg/ml) staining 5HT1B in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of membrane compartment of the mature seminiferous tubules and possible interstitial cells.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

The information provided in the two e-mails is very limited. In the Lab, this antibody has been tested and characterized for Western blot application on human brain tissue lysate and not on SHySy5 cells. You may n...

It is good to know that you have used the Trizol extraction method successfully with other samples. The reason I suggested the negative control sample was because it would, if there is an issue with the antibody specifi...

Thank you for your enquiry. I am sorry to hear this vial of ab13896 is giving you trouble. I am currently reviewing the information you have generously provided and will follow up with you as soon as possible.

Thanks for your patience!

Abcam Scientific Support

Answered on Apr 26 2012

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