Data extracted from this reference:

Inhibitors

Inhibitors

Commentary

Organism

Structure

NADPH

6-phosphogluconate modulates 6PGDH activity. In the forward reaction, it suppresses inhibition by NADP and decreases inhibition by NADPH by increasing the Kd of the reduced coenzyme 6fold it increases the rate of decarboxylation of the 3-keto intermediate , whereas in the reverse reaction, it decreases the Km and removes the slow step

Cyberlindnera jadinii

KM Value [mM]

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.04

-

6-phospho-D-gluconate

dimeric enzyme with two 6-phospho-D-gluconate bound, pH not specified in the publication, temperature not specified in the publication

Cyberlindnera jadinii

0.07

-

6-phospho-D-gluconate

dimeric enzyme with one 6-phospho-D-gluconate bound, pH not specified in the publication, temperature not specified in the publication

reductive carboxylation of ribulose-5-phosphate by 6PGDH is investigated using kinetic isotope effects. In the absence of 6-phosphogluconate, a slow step, which precedes the chemical process, is rate-limiting for the reaction, whereas in the presence of 6-phosphogluconate, the rate-limiting step follows the isotope-sensitive step. Full activity of 6PGDH is achieved when one subunit carries out the catalysis and the other subunit carries an unreacted 6-phosphogluconate (allosteric activator)

Cyberlindnera jadinii

Substrates and Products (Substrate)

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

6-phospho-D-gluconate + NADP+

6-phosphogluconate modulates 6PGDH activity. In the forward reaction, it suppresses inhibition by NADP and decreases inhibition by NADPH by increasing the Kd of the reduced coenzyme 6fold it increases the rate of decarboxylation of the 3-keto intermediate , whereas in the reverse reaction, it decreases the Km and removes the slow step

712444

Cyberlindnera jadinii

D-ribulose 5-phosphate + CO2 + NADPH

-

-

-

?

Temperature Optimum [°C]

Temperature Optimum [°C]

Temperature Optimum Maximum [°C]

Commentary

Organism

24

-

assay at

Cyberlindnera jadinii

pH Optimum

pH Optimum Minimum

pH Optimum Maximum

Commentary

Organism

6.9

-

assay at

Cyberlindnera jadinii

Cofactor

Cofactor

Commentary

Organism

Structure

NADPH

-

Cyberlindnera jadinii

Cofactor (protein specific)

Cofactor

Commentary

Organism

Structure

NADPH

-

Cyberlindnera jadinii

Inhibitors (protein specific)

Inhibitors

Commentary

Organism

Structure

NADPH

6-phosphogluconate modulates 6PGDH activity. In the forward reaction, it suppresses inhibition by NADP and decreases inhibition by NADPH by increasing the Kd of the reduced coenzyme 6fold it increases the rate of decarboxylation of the 3-keto intermediate , whereas in the reverse reaction, it decreases the Km and removes the slow step

Cyberlindnera jadinii

KM Value [mM] (protein specific)

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.04

-

6-phospho-D-gluconate

dimeric enzyme with two 6-phospho-D-gluconate bound, pH not specified in the publication, temperature not specified in the publication

Cyberlindnera jadinii

0.07

-

6-phospho-D-gluconate

dimeric enzyme with one 6-phospho-D-gluconate bound, pH not specified in the publication, temperature not specified in the publication

Cyberlindnera jadinii

Substrates and Products (Substrate) (protein specific)

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

6-phospho-D-gluconate + NADP+

6-phosphogluconate modulates 6PGDH activity. In the forward reaction, it suppresses inhibition by NADP and decreases inhibition by NADPH by increasing the Kd of the reduced coenzyme 6fold it increases the rate of decarboxylation of the 3-keto intermediate , whereas in the reverse reaction, it decreases the Km and removes the slow step