Abstract

The nucleus of eukaryotic cells contains the genome surrounded by a nuclear envelope (NE) consisting of two lipid membranes
with embedded nuclear pores and an underlying nuclear lamina. The uniformity in size and density makes it possible to isolate
pure intact nuclei at high yields from tissue homogenates by centrifugation through a sucrose cushion. NEs can be prepared
from isolated nuclei by enzymatic degradation of their nucleic acid content. The resulting NE preparations contain structurally
well‐conserved inner and outer nuclear membranes with attached ribosomes, nuclear pore complexes and nuclear lamina. Reliable
methods for preparation of nuclei and NEs play an important role in the successful identification of components and their
interacting partners that are located in nuclei and in nuclear subcompartments.

Key Concepts

Pure intact nuclei can be isolated at high yields from tissue homogenates, and nuclear envelopes can be prepared from the
isolated nuclei.

The preparation of pure nuclei is important for the identification of nuclear and subnuclear components and their interacting
partners.

Animal behaviourists must participate in conservation planning to protect the future of biodiversity.