FLAG Antibodies

The FLAG system uses three monoclonal ANTI-FLAG antibodies to detect and purify FLAG fusion proteins. While each recognizes and binds to the FLAG epitope, they exhibit different specificities that depend on the position of the FLAG peptide in the fusion protein. Use the Anti-FLAG® antibodies for immunoblotting, immunoprecipitation, ELISA and other immunodetection techniques.

Affinity gels (antibodies conjugated to agarose) are useful for affinity purification, and can be used repeatedly. Select the gel based on the position of the FLAG and the elution protocol you choose. ANTI-FLAG M1-agarose can be eluted with EDTA; both M1- and M2-agarose can be eluted with FLAG peptide or with glycine-HCl buffer, pH 3.5.

Pure FLAG peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) can be used to competitively displace FLAG-fusion proteins and elute them from FLAG affinity gels. This is a particularly mild elution, with negligible potential for damaging the fusion protein.

The table summarizes the components of the FLAG system that are used in detection and purification of fusion proteins.