RE: [Histonet] Universal (protein) block for IHC

From:

Philip Oshel

Kathy,
Casein is the main protein in milk. Skim milk has lots more stuff in
it than just casein, and who knows what that's doing to your sections?
Phil
>I thought casein was just a fancy name for skim milk. I use it
>sometimes as an avidin block by making a 5% solution out of cheap dried
>milk.
>
>Kathy
>
>-----Original Message-----
>From: histonet-bounces@lists.utsouthwestern.edu
>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly D
>Mcqueeney
>Sent: Wednesday, April 06, 2005 4:42 PM
>To: Johnson, Teri
>Cc: Histonet
>Subject: Re: [Histonet] Universal (protein) block for IHC
>
>Hi Teri,
>I noticed a decrease in specific staining when I plugged it into my
>protocol. I made it myself and it was a big pain. Buy it! Try increasing
>
>antibody concentration on a few slides and see what happens. I never use
>
>a block with my secondary.
>
>Good luck!
>
>Johnson, Teri wrote:
>
>>Has anybody converted to using a universal protein block (casein) for
>>their immunohistochemistry protocols rather than using species specific
>>normal sera? For those who have made the conversion, did you have to
>>make any changes to your methodology or did you just plug it in to your
>>protocol and run? Did you notice any difference in the staining
>>intensity for known antibodies (stronger, weaker, no change)? Are you
>>also using it as a secondary antibody diluent or for washes? Finally,
>>are you using commercially available reagent or are you making your
>own?
>>
>>Thanks so much!
>>
>>
>>Teri Johnson
>>Managing Director Histology Facility
>>Stowers Institute for Medical Research
>>1000 E. 50th St.
>>Kansas City, Missouri 64110
>>tjj@stowers-institute.org
>>
>>
>>
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>>
>
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--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
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