The objective of the present study is to evaluate the effect of cationic dextran on the proliferation rate and biosynthetic activities of HT29, a human colonic adenocarcinoma, and MCF7, a human breast cancer cell line. Cationic dextran was prepared by means of reductive-amination between oxidised dextran and the natural oligoamine, spermine. Biological evaluations including cell proliferation assay, and cell cycle were studied. Flow cytometery was performed in order to determine the biological behaviour of cationic dextran after internalised into the cells. Our results clearly indicated that the cationic dextran was not toxic to the cells when the concentration was 5 μg/ml or lower. The results of the cell cycle flow cytometery indicated that the means of R2 in HT29, MCF7 and HeLa cells were less than 5 three days after treatment with 5 μg/ml of cationic dextran. We conclude that the toxicity of cationic dextran is dose dependent and it is not toxic at concentration lower than 5 μg/ml, and tolerable by the cells, and it can be used as a tool for gene delivery.

Most popular related searches

Related articles

Soluble microbial products, consisting of protein, carbohydrate and humics, are generally considered as the main membrane foulants during the performance of membrane bioreactors. Nitrate and nitrite have been proved to affect the determination of carbohydrate when anthrone-sulfuric acid photometric method is used. In this study, three chemical analytical methods based on photometric assay, including the standard curve method, conventional standard addition method and H-point standard addition method, were...

Alkaline Phosphatase Enzyme Activity Measurement as a Robust on-line Monitoring Assay for Water Quality Assessment.
Zina Al-Saar 4th year Intern student Avans University of Applied Science - ATGM Department Joep Appels - Supervisor and Managing Director microLAN on line Bio-monitoring System.
Many heterotrophic microorganisms maintain their growth by producing enzymes in order to provide low molecular weight inorganic compounds. These compounds could be up taken into the cells from high molecular weight...

For the 2014 World Cup and the 2016 Olympic Games, Brazil has expanded its airport infrastructure. This will lead to an increase in wastewater generation from aircrafts. This wastewater is traditionally taken from the aircrafts and disposed in the public sewage collection system. However, this residual water may have a different composition than the usual sanitary sewage. Therefore, it is important to study an alternative to treat this kind of wastewater. Thus, the objective of this study was to characterize and...

Desalination of seawater is becoming an important means to address the increasing scarcity of freshwater resources in the world. Seawater has been used as drinking water in the health, food, and medical fields and various beneficial effects have been suggested, although not confirmed. Given the presence of 63 minerals and trace elements in drinking desalinated seawater (63 DSW), we evaluated their effects on the behavior of tumorigenic and nontumorigenic cells through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl...

The aim of this paper is to prove the applicability of Auxipó as an auxiliary agent of coagulation for pulp and paper industry wastewater. Thus, it is possible to clarify the effluent more efficiently before launching it in waterbodies. To test the applicability of the auxiliary agent of coagulation, two coagulation and flocculation assay diagrams were developed: the first diagram only shows coagulant dosages, and the second diagram represents the coagulant and Auxipó dosage. Both tests demonstrated efficiency in...

Customer comments

No comments were found for In vitro intracellular trafficking of biodegradable nanoparticles dextran-spermine in cancer cell lines. Be the first to comment!

Add your comment

Great! comment successfully added!

Contact

Your message:

Your email

Your first name

Your last name

I would like to receive periodic email updates and special offers from select suppliers.