First Appeared: Lion (various issues between 26 June 1965 - 26 April
69)

Tarantula
- a petty hoodlum who donned a spider-like costume and took on an arachnid
motif. This unfortunately drew the attention of the Spider, who soon brought
him to justice.

The Snake
- Emil Fangley had a fascination for snakes, and even set up a tourist attraction
called Snake Village. After a very short while the place was bankrupt, and
the bitter Fangley, inspired by witnessing a battle between the Spider and
the Tarantula, decided to don a costumed identity and take revenge on the
society that had spurned him.

The Death-Master - a highly paid assassin hired to
kill the Spider by Crime Incorporated (they tried this before with the
Exterminator). He used high tech weapons,
skullduggery, and a remote controlled killer robot, but none of these could
save him from defeat at the hands of the Spider.

The Red Baron - a madman who commanded a gigantic,
high-tech airship and threatened to destroy New York if his demands for a
billion dollars of gold were not met. The Spider took this threat to the
city personally, and swiftly dealt with him.