Materials

Basic Protocol 1: Image Acquisition

Materials

Cells

Appropriate cell medium

Acid Blue 9 (AB9) in sufficiently pure form can be obtained from TCI America (if using the chemical from other suppliers,
verify that it is nontoxic for cells at the working concentration and that it does not change the pH of the medium)

Glass slides and coverslips can be obtained from numerous vendors, such as Fisher Scientific (http://www.fishersci.com), Tedd Pella (http://www.tedpella.com/), or Electron Microscopy Sciences (https://www.emsdiasum.com/)