Bottom Line:
Identical allelic patterns among various grades of differentiation were found in eight cases.Accumulation of allelic losses from high to lower differentiated portions was found in seven cases and accumulation of allelic losses from low to higher differentiated portions was found in five cases.Various allelic patterns among various grades of differentiation were found in three cases.

ABSTRACTWe performed allelotyping analysis at nine regions on chromosome 3p using 56 microdissected samples from 23 primary lung adenocarcinomas to examine the process of progression within individual lung adenocarcinoma with various grades of differentiation. Identical allelic patterns among various grades of differentiation were found in eight cases. Accumulation of allelic losses from high to lower differentiated portions was found in seven cases and accumulation of allelic losses from low to higher differentiated portions was found in five cases. Various allelic patterns among various grades of differentiation were found in three cases. These results suggested that allelic losses on 3p play an important role in morphological changes of lung adenocarcinomas. We also investigated the relationship between allelic losses on 3p and histological subtypes of lung adenocarcinoma. The frequencies of allelic losses at 3p14.2 and telomeric region of 3p21.3 were higher in papillary type tumour (nine out of 14, 64% and 11 out of 15, 73%) than in bronchioloalveolar carcinoma-type tumour (one out of 8, 13%; P=0.031 and four out of 12, 33%; P = 0.057). These results indicated that allelic losses at 3p14.2 and telomeric region of 3p21.3 are related to pattern of the proliferation of lung adenocarcinoma.

fig3: Left: diagram of the short arm of chromosome 3 (3p) showing the nine microsatellite markers used in the allelotyping analysis. Their order and approximate locations are derived from the Genome Database. Right: summary of all 3p allelotyping results with nine microsatellite markers in lung adenocarcinoma. Solid circles, LOH; open circles, retention of heterozygosity; grey circle, noninformative; hatched circles, homozygous deletion. For each individual tumour analysed, the case number and corresponding grade of differentiation are indicated above the lanes. W=well-differentiated portion; M=moderately differentiated portion; P=poorly differentiated portion.

fig3: Left: diagram of the short arm of chromosome 3 (3p) showing the nine microsatellite markers used in the allelotyping analysis. Their order and approximate locations are derived from the Genome Database. Right: summary of all 3p allelotyping results with nine microsatellite markers in lung adenocarcinoma. Solid circles, LOH; open circles, retention of heterozygosity; grey circle, noninformative; hatched circles, homozygous deletion. For each individual tumour analysed, the case number and corresponding grade of differentiation are indicated above the lanes. W=well-differentiated portion; M=moderately differentiated portion; P=poorly differentiated portion.

Bottom Line:
Identical allelic patterns among various grades of differentiation were found in eight cases.Accumulation of allelic losses from high to lower differentiated portions was found in seven cases and accumulation of allelic losses from low to higher differentiated portions was found in five cases.Various allelic patterns among various grades of differentiation were found in three cases.

ABSTRACTWe performed allelotyping analysis at nine regions on chromosome 3p using 56 microdissected samples from 23 primary lung adenocarcinomas to examine the process of progression within individual lung adenocarcinoma with various grades of differentiation. Identical allelic patterns among various grades of differentiation were found in eight cases. Accumulation of allelic losses from high to lower differentiated portions was found in seven cases and accumulation of allelic losses from low to higher differentiated portions was found in five cases. Various allelic patterns among various grades of differentiation were found in three cases. These results suggested that allelic losses on 3p play an important role in morphological changes of lung adenocarcinomas. We also investigated the relationship between allelic losses on 3p and histological subtypes of lung adenocarcinoma. The frequencies of allelic losses at 3p14.2 and telomeric region of 3p21.3 were higher in papillary type tumour (nine out of 14, 64% and 11 out of 15, 73%) than in bronchioloalveolar carcinoma-type tumour (one out of 8, 13%; P=0.031 and four out of 12, 33%; P = 0.057). These results indicated that allelic losses at 3p14.2 and telomeric region of 3p21.3 are related to pattern of the proliferation of lung adenocarcinoma.