Cas-Designer

Cas-Designer provides all possible RGEN targets in the given input sequence (e.g. exon), with the useful information including potential off-target numbers within 2nt mismatches and optional 3nt bulgeviaCas-OFFinder, and out-of-frame scores viaMicrohomology-predictor. If you want to search with more mismatch numbers or bigger bulge size, then you can download and use a standalone edition of Cas-Designer.