Today’s featured performances include special guest stars like Neil Patrick Harris and Charice, and music from such superstar artists as Madonna, Michael Jackson, and Britney Spears. When Round 1 is done, however, only 32 of the 64 musical numbers in our brutal, single-elimination tournament will still be in contention. Yes, Gleeks, we know it’s not easy narrowing down the field to settle the debate over which song-and-dance interlude ranks at No. 1 in the two-season history of Fox’s smash high-school dramedy, but it’s your patriotic(ish) duty to vote!

In fact, every day around 4:00 p.m. ET over the next few weeks, we’ll launch a new set of bouts that will have you mentally humming your favorite Glee moments and anxiously wondering how in the heck you’re gonna decide between ’em. So make sure the right choice is made by heading down to our polls to vote for your favorites (Round 1 voting will be open for 72 hours), and hit the comments to justify your picks.