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Description:
AAV8-hSyn-DIO-mCherry is a pre-made rAAV in
serotype 8 which will Cre dependently over-express mCherry driven under human
synapsin promoter as illustrated as the cartoon below.
mCherry is a red monomer which matures extremely rapid, making it possible to
see results very soon after activating transcription. It is highly photostable
and resistant to photobleaching.
In the DIO scenario, the transgene of interest is inserted in reverse
orientation relative to the 5' promoter and is flanked by oppositely oriented
loxP and lox2272 sites. In the absence of Cre expression, the transgene
will not be produced. In the presence of Cre expression, the transgene will be
"Flip-exchanged" or FLEXed, leading to the expression of the transgene. This is
due to a permanent Cre-mediated recombination/inversion of the flanked transgene.
This arrangement is called DIO (double-floxed inverse ORF), Cre-ON, Flex-rev
(reverse), Flex-ON/FlexON, or DIO-AAV/AAV-DIO (double-floxed inverse ORF in AAV).