Humanhyaluronidases (HYALs) are a group of five endoβ-N-acetyl-hexosaminidases thatinclude HYAL1, HYAL2, HYAL3, HYAL4, and SPAM1 (PH20). While HYAL1, HYAL2 andSPAM1 are endoβ-N-acetyl-glucosaminidase that are mainly active on hyaluronanwith little activity on chondroitin sulfate, HYAL4 is the only known endoβ-N-acetyl-galactosaminidasethat is mainly active on chondroitin sulfate (type C and D in particular)with no activity on hyaluronan. Hyaluronan and chondroitin sulfate areabundant extracellular matrix components that have numerous biologicalfunctions.

Source:

Chinese HamsterOvary cell line, CHO-derived.

Form:

Supplied as a 0.2 μmfiltered solution in Tris, NaCl and Glycerol.

N-terminalSequence:

Cys34

MolecularWeight:

49 kDa

Activity:

Measuredby its ability to hydrolyze S-35 labeled bovine cartilage chondroitin sulfate< 500 ng of rhHYAL4 is required for the 50% hydrolysis of 10 μgchondroitin sulfate, as measured under the described conditions.

Endotoxin Level:

<1.0EU per 1μg of the protein by the LAL method.

SDS-PAGE:

66-85kDa, reducing conditions.

Purity:

>95%,by SDS-PAGE under reducing conditions and visualized by Colloidal CoomassieBlue stain at 5 ug per lane.