2. Wash cells or tissue with PBS approximately three
times.
Roche kit is used as follows:

3. Put on TUNEL mixture (minimal volume per section),
place a coverslip over sample to spread the volume, and incubate in
humid chamber at 37oC for 30 min.
**TUNEL mixture: (vortex after each addition)

4. Remove slides from incubator and wash off TUNEL
mixture thoroughly with 1X PBS
5. Put on streptavidin secondary (488 or Cy3) for 30
minutes room temperature.
6. Wash 3 times with 1X PBS
7. At this point you can label with additional
antibodies. Minimally, samples should be counterstained with Hoechst or
other nuclear dye. Proceed as directed for cell or tissue labeling.
Otherwise coverslip with Gelvatol.