アプリケーション

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション

Abreviews

特記事項

IHC-FoFr

Use at an assay dependent concentration.

ICC/IF

1/200.

IHC-P

1/10000.

RIA

1/100000.

ターゲット情報

関連性Neurophysin 1 specifically binds oxytocin. Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland.
Oxytocin is a posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin I. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream.
Oxytocin contracts smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions.

Minipigs were deeply anesthetized with a combination of midazolam and
ketamine, prior to transcardial perfusion with phosphate buffered 4%
paraformaldehyde (pH 7.4). After perfusion, the brains were removed with special care taken to preserve the optic chiasm and the median eminence. Blocks of tissue containing the hypothalami
were dissected, postfixed in the same fixative for 1 day and subsequently cryoprotected in 30% sucrose for 3–4 days, prior to freezing. 10 series of 40-mm thick
coronal (6 animals), sagittal (1 animal), and horizontal (1 animal) cryostat
sections were collected. Coronal sections for immunohistochemistry were maintained at -18°C as free-floating sections in a cryoprotectant poly-ethylene glycol solution for up to four weeks.
Immunohistochemistry was performed using the avidin-biotin method. Accordingly,
free-floating sections were
first rinsed in Tris-buffered saline (TBS; 0.05 M; pH 7.4) for 15 minutes. Incubations with
avidin (0.1%) and

ab2078 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab2078 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Thank you for your inquiry.
Unfortunately, we are unable to track down the particular antibodies the authors used when it is not specifically mentioned in the paper.
However, the following antibodies might have been used:
ab39363: ...

Thank you for your enquiry.
I am sorry but we do not have an online RIA protocol. However, I would like to recommmend the following resources:
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=716433&dopt=Abstract
...

Thank you for your enquiry regarding our antibodies ab2078 and ab8745.
Unfortunately we do not have any further information regarding their cross reactivity with vasotocin or isotocin, this has not been tested in our laboratories.
I'm very sorry ...