In this study, biodegradable cassava starch/poly (vinyl alcohol) (PVA) trays with and without poly (lactic acid) (PLA) coating were evaluated as possible substitutes for expanded poly (styrene) (EPS) trays in strawberry packaging. The parameters evaluated were physical and chemical parameters of pH, soluble solids and titratable acidity for strawberries, strawberry and trays moisture content and the mass loss of the systems. In addition, the amount of molds and yeasts was determined in both strawberries and trays. None of the trays used in this study caused changes in the physical and chemical parameters of the strawberries. The molds and yeasts growth was higher in the strawberries packed in EPS compared with the other packaging. The trays with PLA coating and the EPS trays had statistically equal mass loss. The PLA coating applied on cassava starch-based trays was efficient as moisture absorption barrier.