Abstract In order to systematically survey wildlife resources in Kuankuoshui National Nature Reserve of Guizhou Province, infrared cameras were placed in the northern part of the nature reserve within systematic sampling grids (1km × 1km). Key results included: 1) Four species were recorded for the first time which was very helpful to the local biological inventory, including Garrulax maesi, Luscinia cyane, Turdus boulboul, and Dremomys pyrrhomerus (newly recorded in Kuankuoshui NR). 2) The dominant species, with high picture rates (PR), included Chrysolophus pictus (PR: 1.09%), Bambusicola thoracica (0.44), Dremomys pyrrhomerus (3.16), Sus scrofa (0.69), and Muntiacus reevesi (0.49). All of these species had different activity patterns. Sus scrofa showed one main peak at 11:00-13:30 and a secondary peak (08:00-09:00). Muntiacus reevesi showed two activity peaks at 09:30-11:30 and 17:00-19:00. Dremomys pyrrhomerus did not have obvious peaks but was more active from 08:00-10:00. Bambusicola thoracica had several activity peaks, and Chrysolophus pictus was very active at 10:00-11:00 and 16:00-17:00. 3) Neither vegetation type nor elevation showed a significant influence on PR in the monitoring area. Through camera monitoring with systematic sampling, the general situation of biodiversity of birds and mammals in this Nature Reserve has been reflected, which are benefits for local biological inventory, conservation policy-making, and the further ecological researches on species.