assistant professor Dept of Physiology/ Herbal Medicine Research Center, School of Medicine; Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran

Abstract: (11562 Views)

Background
and Objective:Since past
time, herbal plants have been used for the treatment of different abnormalities
such as gastrointestinal disorders. One of these herbswhich used
in Iranian traditional medicine is Trachyspermum ammi. There are many
biological studies conducted forthe determination of its pharmacological properties. The
present study was designed to find out the effects of methanolic fraction
derived from Trachyspermum ammi essense on mechanical activity of the
isolated rat’s ileum.

Materials and Methods: In this laboratory study, for evaluation of
spasmolythic property offraction,
different doses of the solution were added to organ bath after acetylcholine
with concentration of 10-4 molar (M) and for assesment of
antispasmodic effect of fraction, different doses of the solution were added to
the organ bath before acetylcholine with concentration of 10-9 upto
10-2 M, then isotonic contraction ofileum were recorded through an isolated tissue chamber in an organ bath
by using isotonic transducer and oscillographic device. The results were
compared with thymol as a positive control.

Results: Our findings showed that different concentration of
metanolic fraction of Trachyspermum ammi 0.002, 0.005 & 0.01%on acetylcholine 10-4 Minduced contraction in rat’s ileum resulted
in reduced contraction by the essense from concentration of 0.002% and higher.
So that the concentration of 0.002%was
reduced to 59.8% (P-5
M & 10-4 M as compared with metanolic fraction of 0.002, 0.005
& 0.01%had no significant effect on
acetylcholine induced contraction.