Descriptions

Peptide-phosphorodiamidate morpholino oligomers (PPMOs) are synthetic
DNA mimics that bind and silence gene targets. Through designing PPMOs that
silence essential or resistance genes in pathogens, these antisense oligomers could be
utilized as novel antimicrobials. Towards this end, my thesis employed minimum
inhibitory concentration assays (MICs) to identify PPMOs capable of inhibiting in
vitro growth of E. coli. Quantitative real-time PCR was then used to elucidate PPMO
mechanism through investigating transcriptional effects of PPMO treatment. Lastly,
tissue culture cytotoxicity tests were performed to ensure PPMO were benign for
human cells. My thesis has identified multiple PPMOs capable of silencing the
essential gene acpP and the resistance gene NDM-1, establishing a foundation for
both independent and combination PPMO antibiotic treatments. My work has also
shown PPMO treatment to not significantly affect transcription of target genes,
suggesting PPMOs to exert their effects entirely through translational regulation.
Finally, the tissue culture assays demonstrates PPMOs to be nontoxic to human cells.
Taken together, these data indicate PPMOs are a safe, adaptable, and effective new
option for antibiotic therapy.

description.provenance : Rejected by Julie Kurtz(julie.kurtz@oregonstate.edu), reason: Rejecting for reasons listed -
1) Change the commencement date on the bottom of the title page to read -
Commencement June 2014
2) Approval page remove the names, except yours, but remove your middle initial so your name appears exactly the same through your thesis. The signatures lines should read -
Major Professor, representing Microbiology
Chair of the Department of Microbiology
Dean of the Graduate School
3) The actual thesis doesn't have page numbers.
4) The thesis looks like it's in track change mode with lots of words red and underlined.
Once revised, log back into ScholarsArchive and go to the upload page. Replace the attached file with the revised file and resubmit.
Thanks, Julie on 2014-06-20T21:38:03Z (GMT)