Re: HYB: Ploidy speculation

My goodness!
Eloy Boon, Iribov, The Netherlands said:
<During analyses we found that the DNA content of the diploid parents
is different. The hybrid has intermediate DNA content. If I receive
unknown material I can tell on the basis of DNA content what is the
constitution of the material: A triploid with 2 sets of tingitana has a
different DNA content as an triploid with 1 set of tingitana....
The costs of the analyses of ploidy level that our laboratory charges
per sample is approx between 2 and 4 US$ plus costs per order. >
How much does it cost to evaluate the DNA content and would you likely
be able to use the same techniques for bearded irises as for Dutch
irises? For instance, I would like to know how much similarity of DNA
content there it in some cultivars I grow:
1) HONORABILE versus I. variegata
2) I. kashmiriana versus a local cultivar
I would also like a definite ploidy count on the pallida I grow here
that I've used in crosses with modern TBs.
Thanks very much for the reply - please let me know if you might
consider attempting such tests for bearded irises & how much it might
cost. Also, what might be the costs for shipping material from the US
to The Netherlands, what amount of material would be needed, & in what
condition (i.e., a piece of rhizome, active shoot or root meristem,
etc).
Linda Mann east Tennessee USA zone 7/8
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