​​HiCap Neutralizing Broth

Collection broths play a critical role in the success of an environmental monitoring program. HiCap (High Capacity) Neutralizing Broth is the first collection broth developed specifically for use in the food and beverage industry. By neutralizing residual sanitizers on the sampled surface and maintaining the viability of the collected microorganisms for up to 72 hours, HiCap helps prevent false negative test results and facilitates accurate quantification of microorganisms.

Hold samples up to 72 hrs

Unlike other collection solutions, HiCap has demonstrated the ability to maintain the viability of even stressed and injured cells for up to 72 hours after collection. Protect the integrity of your environmental samples. Avoid costly delays and the need to re-sample by using World Bioproducts’ sampling devices hydrated with HiCap Neutralizing Broth.

Request a copy of the collection solution comparison studies that show the HiCap advantage.

Microorganism Viability

To ensure the accuracy of pathogen detection and quantitative test results it is important to maintain the viability of the collected organisms until the sample is processed by the laboratory, typically 1-2 days.

HiCap was formulated to recover and maintain both gram-negative and gram-positive organisms.

Sanitizer Neutralization

HiCap Neutralizing Broth has superior neutralizing capabilities for all sanitizers commonly used by food and beverage processors including quats, peroxide/peroxyacetic, chlorine and chlorine compounds, phenolics, alcohols and acid sanitizers. For companies that employ a rotation of sanitizers to minimize selection of resistant organisms, it is especially important to confirm that the collection broth utilized in their sampling program has the ability to neutralize all of the sanitizers at all of the concentrations utilized in the production facility. ​

Appropriateness for Use

HiCap Neutralizing Broth was developed to provide an alternative to the traditional collection broths (Neutralizing Buffer, D/E Neutralizing Broth, and Letheen Broth), each of which suffers from some deficiency. HiCap was formulated with only components that are found on the U.S. FDA Everything Added to Food in the United States (EAFUS) list as materials acceptable for inclusion in food. There are no meat peptones or extracts and all components are considered non-allergenic. HiCap is compatible with all established rapid test methods including both immunoassays and molecular methods for pathogen detection as well as 3M Petrifilm. ​

Characteristic

HiCap NeutralizingBroth

Neutralizing Buffer

Letheen Broth

D/E Neutralizing Broth

Quat Neutralization1

Highest

Moderate

Weakest

Highest

Free Chlorine Neutralization

Highest

Weakest

Moderate

Highest

High Acid Neutralization

Highest

Weakest

Moderate

Highest

Peroxyacetic Acid Neutralization

Highest

Weakest

Weakest

Highest

All components listed in EAFUS2 as acceptable for use in food

Yes

No

Yes

No

All components compatible with 3M PetrifilmTM 3

Yes

No4

Yes

No

All components identifiedas non-allergenic

Yes

Unknown5

Unknown6

No7

Compatible with moleculardetection assays

Yes

No8

Yes

Yes

Stable at ambientstorage temperature

Yes

Yes

Yes9

No10

​ 1. Technical data available upon request. 2. EAFUS - Everything Added to Food in the US (USFDA) 3. 3M and Petrifilm are trademarks of 3M, St. Paul, MN 4. 3M recommends avoiding solutions that contain bisulfite, citrate and thiosulfate when working with Petrifilm 5. Contains aryl sulfonate complex of unknown allergenicity 6. Letheen Broth contains lecithin. Lecithin used in microbiological culture media is typically derived from soy or egg 7. Contains peptone from milk and lecithin, which is typically derived from soy or egg 8. Possibly due to the presence of aryl sulfonate complex in the neutralizing buffer 9. Some suppliers of Letheen Broth specify refrigerated storage 10. D/E shows pH instability with ambient storage.