The TOS cards included questions about the first six Star Trek films while the TNG cards included questions about Star Trek Generations and Star Trek: First Contact. The DS9 cards covered all seven seasons but due to the game's release date, the Voyager cards only incorporate events up until the end of its season six.

The object of the game was to circle the board while landing on spaces to answer questions in five possible categories:

Tricorder Readings were questions based on events, objects and story lines

Q Continuum questions were General Knowledge questions, often delving into behind-the-scenes or all-over Trek-related questions

Answering a question about a series in that series' quadrant earned an insignia marker in that series' color. Correctly answering a Q Continuum question also let the player move any ship (including their own) to any other space on the board while answering it incorrectly forced you to the nearest Borg Space (see below).

Engage! permitted the player move to any space of the same color as the Engage! space

Lock Phasers! let the player challenge another player for one of their insignia markers

Borg Spaces forced the player to "defend" their last-earned insignia marker by answering another question about the marker's corresponding Trek series

Getting at least one of each of the four Starfleet insignia markers allowed the player to use a Wormhole space to move to the center Starfleet Command space, and required the player to answer one more series and category question (chosen by the player's opponents) to win the game.