Discovery Zr Carbon HPLC Columns

Discovery Zr-Carbon consists of spherical, porous carbon-coated zirconia particles. It is ideal for the reversed-phase separation of positional isomers, diastereomers, and many other compounds. It complements the selectivity offering of the other zirconia-, silica-, and polymer-based Discovery phases, and allows the use of the full range of mobile phase pH from pH 1 to 14. It is a great alternative when C18 does not work.

Particle:

Zirconia (ZrO2)

Bonded Phase:

Carbon

Particle Shape:

Spherical

Particle Size:

3 and 5µm

Pore Size:

300Å

Surface Area:

30m2/g

pH Range:

1 to 14

Temperature Range:

up to 100°C in standard hardware (up to 200°C in custom all-steel hardware)

Unique retention and selectivity for method development at pH and temperature extremes

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Very different selectivity than any other RP material makes it good for orthogonal screening

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Excellent separation of geometric isomers and diastereomers

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Very hydrophobic surface

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Most different retention compared to other Discovery Zr phases for non-ionic compounds

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Similar to porous graphitic carbon, but with added ion-exchange interactions

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pH stable from 1-14

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Thermally stable up to 200°C

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Avoid fused-ring aromatics as they are too strongly absorbed by Discovery Zr-Carbon

Here's the

Proof

SEPARATIONS OF STRUCTURAL ANALOGS

Carbon-based packings have found a niche within the population of HPLC supports. The main benefits of carbon over silica are enhanced chemical and thermal stability, and the ability to separate positional isomers. Compounds that have the same hydrophobicity, but different molecular shape, can be separated on the rigid carbon surface but not on phases that contain flexible ligands, like C18-bonded silica. In the figure below, the analogs ethylbenzene and p-xylene co-elute on the non-carbon Discovey Zr-PBD phase, but are resolved on Discovery Zr-Carbon.

ISOMERS AND DIASTEREOMERS

The ability of Discovery Zr to distinguish positional isomers is demonstrated in the figure below. The isomers co-elute on a C18-silica column, but are resolved on the Discovery Zr-Carbon column.

DISCOVERY Zr-CARBON HAS THE MOST DIFFERENCE FROM OTHER DISCOVERY Zr PHASES

The figure below shows a selection of twenty three different non-ionic probes. Each was run on the four Discovery Zr phases. Retention relative to benzene was plotted. For these compounds, the Discovery Zr-Carbon has the most unique selectivity.