Re: [Histonet] Lung processing

From:

Rene J Buesa

Colin:
You hit "the nail in the head". It is "vacuum thing", I don't know if it is a "Thermo thing".
Check their operating manual and find out the vacuum level (mm of Hg) and compare with what the VIP 1000 used to have. If the vacuum is higher in the VIP 1000, that is your answer, if it is the same, then it has to be a "Thermo thing".
René J.
"Weaver, Colin" wrote:
Hi - everyone we have just moved from routine processing in a VIP 1000
to a Thermo-Shandon Excelsior. This has been working perfectly except
when processing lung, especially spongy lung. When embedding after
processing wax infiltration appears to be patchy, bubbles come out of
the tissue when pressing the tissue into a mould, after trimming there
are "holes" in the tissue, and sections often crumble because of lack of
wax support. We have vacuumn on everything from first alcohol onwards.
Anyone got any suggestions as to why this is happening? We used to use
Thermo-Shandon Hypercenter XP's and had the same problem with lung, is
it a Thermo thing?
Colin
Histology Dept
VLA Thirsk
Sunny Yorkshire
Veterinary Laboratories Agency (VLA)
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