Fw: Help with clarification of "Lake"

From:

"Histo Net" <histonet@histosearch.com>

-----Original Message-----
From: NFitzgi726@aol.com <NFitzgi726@aol.com>
To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
Date: Tuesday, September 12, 2000 3:26 AM
Subject: Help with clarification of "Lake"
>Me and my fellow coworkers are studying for our ASCP HT and we were
wondering
>if anyone out there could help us with some clarification about a lake for
>cytoplasmic staining. We know that a Lake is the combination of a mordant
>with a dye and that the dye lake can bind to tissue components and form an
>insoluble colored deposit. But what we would like some help with is this:
>Why is it that when you use the lake technique procedure you get different
>results from when you stain with the slide standing in a coplin jar versus
>lying flat on a stain rack?
>
>Thanks and please do not laugh real hard and fall of your seat and hurt
>yourself.
>
>Noreen Fitzgibbons
>Reno, NV
>