Fly gurus,
We're wondering if there is any experience using p element
transformation vectors (for example pUAST) made by gateway cloning
(invitrogen). Is there any difference in expression or mobilization
from a p-element having the 20-odd basepair recombination site
internally in the polylinker? We are making p-element transformation
plasmids this way, but would like to know if we are getting ourselves
into trouble.
Thanks,
Tony
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