Abstract - We analyzed two kinds of dairy fat differing in their contents of cis9,trans11-conjugated linoleic acid (cis9,trans11-CLA or rumenic acid), and determined the positional distribution of this CLA-isomer within the three sn- positions of the triacylglycerol. In the high rumenic acid fat (HR), the CLA-isomers amounted to 2.1% of total fatty acids,
and 0.8% in the low rumenic acid fat (LR). Over 90% of the total CLA-isomers were in the form of rumenic acid, with an identical
isomeric CLA distribution in both fats. The two fats differed mainly with regards to their contents in palmitic acid, a-linolenic
acid, and isomers of trans-C18:1. Conversely, our stereospecific determination indicates that the positional distribution of rumenic acid is preserved
among both types of fat, and is more specific for the sn-3 position of the triacylglycerol (54 to 64% of the total rumenic acid). Such a positional distribution is believed to be
nutritionally relevant.