I am doing Western blotting using a PVDF membrane and an amplified alkaline
phosphatase detection system. I am getting high levels of background
staining and am wondering if it is because of the biotin/avidin system being
used. The AP substrate may also be a little old - would this cause
background staining or just less colour production? Thankyou for your help.
-----== Posted via Deja News, The Leader in Internet Discussion ==-----
http://www.dejanews.com/rg_mkgrp.xp Create Your Own Free Member Forum