RE: [Histonet] histoclear vs xylene

From:

"Favara, Cynthia \(NIH/NIAID\) [E]"

Here is the web site on xylenes from the EPA
http://www.epa.gov/ttn/atw/hlthef/xylenes.html
I would not necessarily agree that "it is not even considered Toxic" but
I do agree nothing is quite like xylene or toluene for histo.
c
Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317
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-----Original Message-----
From: Bruce Abaloz [mailto:brucea@unimelb.edu.au]
Sent: Wednesday, June 21, 2006 5:28 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] histoclear vs xylene
>John,
>
>I agree with Rene that Histoclear doesn't work as well as Xylene.
>We used to deparrafinize routinely in Xylene...X2 changes, 3 min.
>each.
>Now we use Histolene/Histoclear, x2 changes of 10 min. each - a
>third wd. probably be good (?) We now have gone back to
>deparrafinize in Xylene for ALL Immuno/Histochem. 'stuff' (only), as
>were having problems with 'end results', until - we went back to
>deparrafinizing in Xylene!
> As far as the "known health hazards" - check your MSDS/Xylene; it
>is not even considered Toxic....simply make sure you do all your
>work under a fumehood/be sensible...I use it to purge my Tissue
>Processer as - NOTHING works like Xylene.
Cheers,
Bruce in OZ
>
>In a "nutshell" I can tell you the following:
> 1-xylene: universally used for tissue processing and staining but
>a product that should be avoided because of known health hazards.
> 2-histoclear: a "xylene substitute" sometimes used as a
>replacement of xylene. Does not work equally well. It is "allegedly
>safe" but really with no documentation of long term health effects
>as xylene has. Maybe with time it will turn out to be as hazardous
>as xylene.
> Our profession has some health hazards and we just have to work as
>safely as possible.
> Hope this will help you!
>
>
>John Campbell
>Graduate Student
>University of Missouri Rolla
>Lab phone 573-341-4069
>Cell phone 573-694-0596
>
>
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--
BRUCE ABALOZ
PH:61383446282
HISTOLOGIST
FAX:61383447909
DEPT. of ZOOLOGY
EMAIL: brucea@unimelb.edu.au
THE UNIVERSITY Of MELBOURNE. VICTORIA. AUSTRALIA 3010
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