PyroMark Q24 Tests

For analyzing sequence variation or methylation of specific genes using the PyroMark Q24 Instrument

Detection and quantification of different mutations in a single run

Flexible software for post-run analysis of adjacent mutations

Quantification of individual or multiple CpG sites in different genes

Estimation of global CpG methylation in defined regions of interest

Sequence context provides built-in control of the assay

PyroMark Q24 Tests are a range of assays suitable for highly reliable, quantitative mutation and CpG methylation analysis and are optimized for use with the PyroMark Q24. Tests designated with 'Q24' are optimized for use with the PyroMark Q24, while others such as PyroMark HFE and PyroMark MTHFR can be used with the PyroMark Q96 ID and PyroMark Q96 MD as well. For more tests for these instruments, see PyroMark Q96 Tests.

Prior to Pyrosequencing, a biotinylated PCR product is generated. Biotinylated PCR products are bound to streptavidin-coated Sepharose beads. The beads are then captured with the vacuum tool on the PyroMark Vacuum Workstation, and throroughly washed, generating single-stranded DNA suitable for sequencing. PyroMark Q24 Tests are predesigned assays for methylation analysis by Pyrosequencing. Each test includes the primers necessary for generating the biotinylated PCR product, as well as the primer for the Pyrosequencing reaction.