T.S.B. (TRYP SOY BROTH) 7,5% SODIUM CHLORIDE

Technical Data #2947a / 2013.11.13

Tryp Soy Broth 7,5% Sodium Chloride is a liquid medium used to differentiate enterococci from other streptococci based on his high concentration of NaCl. Sodium chloride reacts as selective agents because mostly of Enterococci sp. may grow in this selective broth.

FORMULAin grams per liter purified filtered water

Casein Peptone

17,0

Soy Peptone

3,0

Dextrose

2,5

Potassium Phosphate, dibasic

2,5

Sodium Chloride

75,0

pH 7,3 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONSThis medium is for laboratory use only.

STORAGEStore prepared media at 2-8° C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25° C.

SIGN OF DETERIORATIONMedia should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (evaporation or discoloration). Do not use dehydrated media if it is caked.

DIRECTIONSSuspend 30 g of powder in 1000 ml of purified filtered water and add 70 g of Sodium Chloride. Heat with frequent agitation to dissolve. Mix thouroughly and dispense into culture tubes. Sterilize at 121° C for 15 minutes.