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Adult LacZ Expression Data for Plin2 (MCRY)

Overview

Adult expression (≥ 6 weeks): The lacZ reporter gene within
the targeting vector was used to determine the wholemount expression
profile of the targeted gene. Mice were fixed by cardiac perfusion
using 4% paraformaldehyde (PFA). Following dissection, the tissues
to be stained were fixed an additional 30 min in 4% PFA before being
rinsed in phosphate buffered saline and transferred to lacZ staining
solution, containing 0.1% X-gal, for up to 48 hours. After an
additional overnight post-staining fixation in 4% PFA, tissues were
cleared with 50% glycerol then transferred to 70% glycerol for long-term
storage.

Display Criteria

A tick-list of 41 tissues and organs was scored for the presence (green
tick) or absence (red cross) of lacZ expression.

The term 'Ambiguous' was assigned when there was uncertainty as to
the validity of the observed pattern e.g. staining may be very faint,
background or an artefact due to trapping.

The term 'No Data' was assigned if the stained entity was not available
to assess.

By default, up to 27 standard images may be taken using a Leica MZ16A
microscope and Imagic software. These images were only collected when
X-gal staining was present.

If information or images that you are interested in are not presented, please send
details to MGPenquiries@sanger.ac.uk
and, on a limited, first come first served basis, we will process your request.

The adult wholemount LacZ expression protocol has been refined to minimize
background staining however, it can still occur. We have systematically
documented the sites and intensities of this background stain in male and
female wildtype C57BL/6 (C57BL/6J-Tyr<c-Brd>; C57BL/6NTac/Den) mice.
The major locations where background staining may be observed are:

Nasal cavity

Thyroid gland

Glandular stomach

Kidney

Male genitals

Additional background staining is observed occasionally in:

Rib cage

Thymus

Mesenteric lymph nodes

Ovaries

Examples of the pattern and level of background stain are presented below.