JavaScript seems to be disabled in your browser.For the best experience on our site, be sure to turn on Javascript in your browser.

Cookie Policy:This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.

Robustness of NEDD8-Rhodamine 110 substrate: 400nM of NEDD8-Rhodamine 110 was incubated with and without 30pM NEDP1 in a 384 well plate (n=16), and progress curves were normalized to the maximum fluorescence signal to produce “% reaction progress”. The Z’ value, a statistical parameter widely used in the evaluation of screening assays, was calculated at each time-point.

NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8) is a ubiquitin like protein that plays an important role in regulating development and the cell cycle. NEDD8 is conjugated to a target protein via a signaling cascade similar to ubiquitin: a NEDD8 specific E1 activating enzyme (APPBP1/UBA3) adenylates the C-terminus of NEDD8 which is than subsequently passed to an E2 conjugating enzyme (UBE2M or UBE2F) that facilitates covalent attachment of NEDD8 to a cullin subunit of an SCF E3 ubiquitin ligase. NEDD8-Rhodamine 110 can be used as a substrate for enzymes exhibiting deNEDDylating activity, e.g UCH-L3, UCH-L1, COP9 Signalosome and NEDP1.