Optimal dilutions / concentrations should be determined by the end user.

With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.

It has also been reported to be useful for immunoprecipitation of PKC gamma.

Target

Function

This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Tissue specificity

Expressed in Purkinje cells of the cerebellar cortex.

Involvement in disease

Defects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA).