Snippet: “… The study involved 3 laboratories: a biopharmaceutical company (Roche Diagnostics), an academic research laboratory (Leiden University Medical Center) and a vendor of tools for glycan analysis (ProZyme, Inc.). The same mAb sample was analyzed 6-fold on 2 different days. …”

Snippet: “… A rejected ion mass list of common contaminants was used that included m/z = 391.2, 413.2, and 427.2 Da. The ion optics of the mass spectrometer was tuned to a 1 pmol/μL (water) solution of 2-AB G0 glycan obtained from ProZyme, Hayward, California. …”

Snippet: “… Deionized (DI) water, 18 MΩ-cm resistivity or better was used throughout for the sample preparation and analysis. 2AA-labeled glycan standards used for calibration were obtained from ProZyme (Haywood, California)…”

Snippet: “… To identify peaks, including high mannose structures, known glycan standards (ProZyme) were labeled with APTS as described for IgG glycans and spiked into human IgG-derived samples. ProZyme standards and IgG-derived …”

Snippet: “… up to remove excess dye. Identification of the glycans was performed by comparison against a 2-AB labeled Human IgG N-linked library from ProZyme, spiked with 2-AB labeled Man5 standard. The separation of glycans was …”

Product: 2-AB labeled Human IgG N-linked library

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