Overview

SpecificityAntiserum will specifically react with a 60 kDa Cyclin A2 protein from human, rat and mouse tissue. No reaction was observed against other related cyclins. Cross reactivity with Cyclin A from other species may also occur.

Cells were either untreated (left) or treated with 10 µM ceramide (right), fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and incubated in blocking buffer (PBS, 2% skim milk, and 0.1% Tween-20) for 30 min at room temperature. Samples were incubated with primary antibody at 1/50 dilution for 1 hour at room temperature. An AlexaFluor®-conjugated goat anti-mouse IgG (1/1000) was used as the secondary antibody.

Thank you for your enquiry and your interest.
The antibody you mentioned is a polyclonal antibody. Polyclonal antibodies consist of a mixed population of IgGs each of which will recognize a different epitope. Therefore, for polyclonal antibodies no ...

This antibody has only been tested on mammalian sources. It cross-reacts with rat and
mouse. Presumably this molecule is so fundamental, that it is highly
conserved in nature, but it hasn't been tested.

Neil HaywardAbcam Scientific Support

Answered on Aug 21 2001

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"