Sp2/0-Ag14 cells

Catalog #:

C0033005

Shipping advice:

Use only Express Delivery (overnight) for cell line. International shipping rate please contact us for details

Frozen vial

USD302.0

Origin:

The line was formed by fusing BALB/c spleen cells (from mouse immunized with sheep RBCs) with the P3X63Ag8 myeloma. The cells do not secrete immunoglobulin, are resistant to 8-azaguanine at 20 ug/ml and are HAT sensitive.

Species:

Mus musculus, mouse

Morphology:

Lymphoblast-like; hybridoma, B lymphocyte

Properties:

Suspension

Strain:

BALB/c

Medium:

Subculture:

Cultures can be maintained by addition or replacement of fresh medium. Maintain cell density between 5x10^4 and 5x10^5 viable cells/mL. Some cells can attach and be transferred by shaking them loose. Culture at 5% CO2, 37°C. Renew medium once every 2 to 4 days depending on cell density.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO

Biosafety Level:

I

Sterility:

Bacteria: Negative

Yeast: Negative

Mycoplasma: Negative

Citation Format:

If use of this culture results in a scientific publication, it should be cited in the publication as: Sp2/0-Ag14 (AddexBio C0033005)