Overview

SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.

Goat Anti-Rabbit IgG H&L (Biotin) images

Ab97049 was used at dilution 1:500 for IHC analysis of GATA4 expression in paraformaldehyde fixed, paraffin embedded testis sections. The primary antibody was a rabbit polyclonal IgG raised against human GATA4.

As with most antibodies the dilution that works best for you depends on multiple varaibles and so you should do some titrations to find the best for you, but I placed a good starting point next to each. Read More

I am beginning to gather the CoC's you have requested. When you have a chance, please provide the lot number from each catalog number so I can include this information on the documentation. Thanks for the additional information.

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The best way to figure out dilutions and number of tests is to look at the datasheet. In most cases we will have a recommended dilution for each application, and will also include the concentration of the product along...