[Study on antitumor activity and mechanism of Ganoderma polysaccharides B].

Abstract

OBJECTIVE:

To study the antitumor activity and mechanism of Ganoderma polysaccharides (GL-B).

METHODS:

Both in vivo and in vitro experiments were conducted in the study. Proliferation of tumor cells was detected by MTT method, tumor necrosis factor alpha (TNF alpha) was detected by biological assay, and interferon gamma (IFN gamma) by enzyme-linked immunosorbent assay (ELISA). The level of mRNA was detected with the method of reverse transcriptase-polymerase chain reaction (RT-PCR).