This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.

Species and Isotype: Human IgG1, Fc Silent™, lambdaClone Number: CTX-scFv8UniProt Accession Number of Target Protein: Published Application(s): ELISA; neutralizePublished Species Reactivity: South-American rattlesnake Crotalus durissus terrificusImmunogen: scFv 8 was constructed from a phage display library using V-genes from 43 non-immunized donors.Specificity: scFv binds to crotoxin and recognises epitopes overlapping BI-a (an epitopic region at the interface between the CA and CB subunits of the heterodimer) and BIII (an epitopic region on CB involved in the enzymatic activity of CB) - scFv 8 may be able to caused the two subunits to dissociate and suppress the synergistic activity between them. Crotoxin is a phospholipase A2 neurotoxin and is the main toxic component in venome from Crotalus durissus terrificus rattlesnakes, acting by blocking neuromuscular transmission.Application Notes: scFv 8 is able to reduce the myotoxicity induced by crotoxin and is able to neutralize the lethality induced by the toxin. This scFv was also used for (competitive ELISAs).Antibody First Published in: Cardoso et al. Neutralizing human anti crotoxin scFv isolated from a nonimmunized phage library. Scand J Immunol. 2000 Apr;51(4):337-44. PMID:10736105Note on publication: Describes the construction and a phage library using V-genes from non-immunizzed donors. Studies were conducted which determined the binding region of the scFv and the neutralizing effect of the scFv on mycotoxicity and lethality in mice.

Product Code: Ab00760-10.3

Human IgG1

Buy Now

Product Form

Purification: Protein A affinity purifiedSupplied in: PBS with preservative (0.02% Proclin 300)Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°CConcentration: See vial label.

Important note – This product is for research use only. It is not intended for use in therapeutic or diagnostic
procedures for humans or animals.