CytoTune®-iPS 2.0 Sendai Reprogramming Kit

Newly released, the CytoTune®-iPS 2.0 Sendai Reprogramming Kit improves upon the well referenced CytoTune®-iPS Sendai Reprogramming Kit. The CytoTune®-iPS 2.0 Kit is non-integrating and still requires only one overnight incubation compared to multiple days of transductions required for mRNA reprogramming.

This superior CytoTune® 2.0 Sendai system provides:

Increased reprogramming efficiency for even more colonies

Lower cytotoxicity to allow for smaller starting cell populations

Faster clearance to get to your iPSC experiments sooner

The 2.0 Kit contains a polycistronic vector which offers an increased reprogramming efficiency, up to 1.2%. This polycistronic vector has a different backbone containing new temperature sensitivity mutations to the polymerase-related genes, and this helps to clear the virus faster after reprogramming and causes less cytotoxicity to the cells.

CytoTune®-iPS Kit

0.19% efficiency

CytoTune®-iPS 2.0 Kit

0.49% efficiency

Improved reprogramming efficiency. Alkaline Phosphatase staining of iPSCs generated from human dermal neonatal fibroblasts (BJ strain) using (A) the CytoTune®-iPS Sendai Reprogramming Kit at an MOI of 5 and (B) the CytoTune®-iPS 2.0 Sendai Reprogramming Kit at an MOI of 5:5:3 shown at 21 days post-transduction.