Information that pertains to questions below: 4 siblings (parents history unknown except that they were first cousins) all have hearing loss. All are married with unrelated spouses with no hearing loss. Oldest (Female)-has 4 female children with no hearing loss2nd Oldest (Female)-has 2 male children with no hearing loss-1 female child with hearing loss3rd sibling (Male)-2 male children and 1 female child with no hearing lossYoungest (Female)-3 male children and 1 female child all with hearing loss

portion of the normal sequence of the tRNA gene: GCTTTGGGGGGTTCportion of the mutant sequence of the tRNA gene: GCTTTGGGGGGGTTC

Question #1) Only one of the siblings (a female) had 100 percent mutant mitochondrial DNA. Based on the information above, which of the siblings had 100 percent mutant mitochondrial DNA? *I chose the youngest- because of how many offspring were affected ????

Question #2) Why do some of the female siblings with hearing loss have children who do not have hearing loss? (mutations in mitochondrial DNA are usually transmitted in a uniparental-maternal fashion in mammals)a: the females who had offspring without hearing loss were heteroplasmicb: there are two mitochondrial serine tRNA genes. The other tRNA gene sometimes compensates for the loss of function in the mutant tRNA gene.c: Biparental inheritance of mitochondrial DNA caused some of the offspring to escape hearing loss.d: those with hearing loss were segregational petites, whereas those without hearing loss were suppressional petites. *I chose d because the suppressional petites would account for no hearing loss??

Question #3) Which of the following is the most probable cause of this mutation?a: slippage during DNA replicationb: tautomeric shiftc: spontaneous deaminationd: SOS mutagenesis*I chose a because that one seems to be the most likely cause