Isopropanol solutions were then prepared by taking 10 mL of isopropanol (see notes) and putting it in a tube. Serial dilutions with water were then carried out to create 10%, 1% and 0.1% solutions. To create these solutions, 1 mL of solution was added to 9 mL of water. 0.872 mL of each solution was added to its respective film.

Films Prepared

All films were heated on a hot plate until the PVOH dissolved in the water. For films 5,6, and 7, additional water was added to the gel to assist in the dissolution. Once dissolved, the film was removed from heat and any additional reagents were added while the gel was stirred. When isopropyl and glutaraldehyde were added, the isopropyl would be added first and then would stir for roughly 2 to 3 minutes. Glutaraldehyde was added last. Once prepared, the films were poured into a large plastic dish and were allowed to evaporate over the weekend.

Data

No data was collected today

Notes

87.2 μL isopropanol solution corresponds to the amount of isopropanol needed to bind 10% of the OH groups in PVOH. In the serial dilutions, because the concentration has been reduced by a factor of 10%, the same volume (87.2 μL) of the diluted solutions could be added to films