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Abstract

The anesthetic effects of two chemical compounds, lidocaine and sodium bicarbonate, and two natural compounds, clove and thyme extracts, were examined on the Red Blood Cells (RBC), White Blood Cells (WBC), hemoglobin, hematocrit, and blood cortisol levels of Grass carp at two times of 10 minutes and 24 hours after anesthesia. RBC counts had no significant increase in the samples of 10 min and 24 h after induction of anesthesia with clove extract only compared to the control group (p > 0.05). The number of WBC increased significantly in the samples from 10 min and 24 h after anesthesia with sodium bicarbonate only and it was not different from control samples in other treatments 24 h after anesthesia. The amount of hemoglobin indicated an increase with all treatments compared to the control in 10 min after anesthesia. The increases were not significant in thyme and clove extract (P > 0.05). Samples treated at 24h after anesthesia exhibited non statistical hemoglobin elevations with thyme extract only. Hematocrit increased significantly in the samples from 10 min after anesthesia with lidocaine and sodium bicarbonate but, at 24h after anesthesia, hematocrit amount with thyme and clove extracts and lidocaine treatments were not different from the control significantly (P > 0.05). Also, Cortisol level was significantly higher in both sampling times for lidocaine and sodium bicarbonate treatments than the control group. Accordingly, the use of natural anesthetics (thyme and clove extracts) will result in better outcomes than the synthetic compounds (sodium bicarbonate and lidocaine) in the Grass carp.