Cholera disease contributes majorly to health challenges of Benue state, Nigeria.The prevalence of the disease is more than 10% and this places a heavy financial burden on the government. Cholera vaccination is not incorporated in the National Programme of Immunization in Nigeria. Most cases the disease progresses with severe complications and death. The present study assessed the knowledge of, attitude toward and prevention practices of cholera in a sub urban slum of Makurdi, Benue State, Nigeria. A cross sectional study conducted in a cholera prone sub-urban area of Wadata, Benue State. The sample size was 80 and was obtained through multi stage sampling technique where two villages were randomly selected. Data were collected through a structured interviewer administered questionnaires. The study was conducted from June 2016 to September 2016. 80 respondents were analysed. Only 27% could define cholera correctly while 89% were aware of oral rehydration salts (ORS). Forty three percent had good knowledge score of cholera, 96.7% had high positive attitude toward cholera and 65% had good practice of cholera prevention. Knowledge had significant associations with sex, education, attitude and practice. Findings in this study showed that knowledge, attitude and preventive measures of cholera can be improved through health education and community involvement in various environmental challenges in the area by government agencies and non-governmental development organizations.