应用

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用

Ab评论

说明

ICC

Use a concentration of 5 µg/ml.

IHC-Fr

Use a concentration of 4 µg/ml.

IP

Use at an assay dependent concentration.

WB

Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).

EMSA

Use at an assay dependent concentration.

IHC-P

Use a concentration of 4 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

靶标

功能Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.

疾病相关Androgen insensitivity syndromeSpinal and bulbar muscular atrophy X-linked 1Defects in AR may play a role in metastatic prostate cancer. The mutated receptor stimulates prostate growth and metastases development despite of androgen ablation. This treatment can reduce primary and metastatic lesions probably by inducing apoptosis of tumor cells when they express the wild-type receptor.Androgen insensitivity, partial

结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. In the presence of bound steroid the ligand-binding domain interacts with the N-terminal modulating domain, and thereby activates AR transcription factor activity. Agonist binding is required for dimerization and binding to target DNA. The transcription factor activity of the complex formed by ligand-activated AR and DNA is modulated by interactions with coactivator and corepressor proteins. Interaction with RANBP9 is mediated by both the N-terminal domain and the DNA-binding domain. Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain.

翻译后修饰Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.

细胞定位Nucleus. Cytoplasm. Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1.

Anti-Androgen Receptor antibody [AN1-15] 图像

Immunohistochemistry was performed on normal biopsies of deparaffinized Human thyroid tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a Anti-Androgen Receptor antibody [AN1-15] (ab2742) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

ICC/IF image of ab2742 staining human prostate cells expressing androgen receptors. The cells were fixed with 4% paraformaldehyde, permeabilised with 0.8% Triton X-100, and blocked with 20% serum for 1 hour at 24°C. The primary anitibody was diluted 1/50 in 20% horse serum in PBS and incubated for 20 minutes at 4°C. An Alexa Fluor® 488 conjugated donkey anti-rat was used as the secondary antibody.

The blue staining is the cell nuclei, the green staining shows the androgen receptors.

Our online datasheets shows this antibody has been successfully tested in IHC-P using mouse tissue. So we will guarantee the use of the antibody in your stated species and application. For more details regarding ou...

Thank you for your email. Unfortunately I have not received the attachment. Could you please send it again? Can you please confirm also what kind of tissue has the customer tested in the end - also some heart or skeletal muscle? Did the BSA blocking ch...

Thank you very much for having submitted the customers questionnaire. I am very sorry that the customer has experienced problems with this antibody. I have looked through the protocol provided, and would like to make the following suggestions: 1.) I am...

Thank you for your interest.
It is very likely that ab9474 works in mouse but we have no data yet. According to Blast search, the homology between human and mouse AR is very high.
http://www.uniprot.org/blast/uniprot/2011101050RXMELAA8
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Thank you for your enquiry.
It appears the subclass of this antibody is unknown and the product is total IgG. I would recommend using Protein G only because it is the better bead to use for most subclasses of Rat IgGs.
In addition, below you wil...