Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Custom Antibody Production Services

High quality rabbit polyclonal antibodies

LifeSpan offers full service production of rabbit polyclonal antibodies from peptide design to the characterization of the final product. With a decade of antibody production experience and more than 2,500 antibodies synthesized, LifeSpan can help you develop the reagent you need.

Investigators can quickly initiate the production of an antibody by selecting the LifeSpan SureAbTM Synthesis Service or work with LifeSpan to design a more customized protocol to suit their specific needs.

Production projects can be tracked in real time through LifeSpan’s secure online interface, allowing investigators to make adjustments as the protocol develops.

Once produced, antibodies can then be characterized for affinity and specificity by Western Blot analysis or in immunohistochemistry, taking advantage of LifeSpan’s expertise as a molecular pathology provider and extensive tissue bank.

SureAbTM Synthesis

The SureAbTM protocol has been designed to include those features that LifeSpan finds to be most effective in generating antibodies that are optimally sensitive and specific.

SureAbTM Synthesis Protocol

Peptide synthesis (10 mg at 95% purity)

Peptide conjugation to KLH (2 mg)

Duplicate rabbit immunization (13-weeks, 6 boosts)

1 pre-bleed, 3 production bleeds (120 ml sera per rabbit pair)

20 ml affinity-purification of the 1st production bleed

SureAbTM Options

LifeSpan peptide design

Number of peptides per target

Characterization by Immunohistochemistry

Characterization by Western Blot

Additional affinity-purifications

Antibody Characterization

The antibody characterization protocol for each study is custom designed to address the particular needs of the investigator. LifeSpan offers both Western Blot and Immunohistochemistry characterization using tissue and cell lines from its extensive tissue bank or supplied by the investigator.

Immunohistochemistry Analysis

Formalin-fixed and Frozen tissues

LifeSpan supplied normal and diseased human tissues

LifeSpan supplied mouse, rat, dog, and primate tissues

Commercial or client supplied tissues or cell lines

Adrenal, 40X

Western Blot Analysis

LifeSpan supplied human and animal tissue extracts

LifeSpan supplied cell line extracts

Client supplied protein extracts from tissues or cells

Commercially available tissue or cell extracts

Sample Western

Customized Antibody Production

Immunogen Preparation

Peptide design (LifeSpan experience)

Peptide synthesis (variable scale and purity)

Synthesis efficiency testing (HPLC, Mass Spec)

Peptide conjugation (KLH, BSA, Ovalbumin, other)

Conjugation efficiency testing (Gel shift assay)

Immunogen types (peptide, whole protein)

Immunizations

Number of animals immunized

Rabbit, other species (goat, etc.)

Flexible immunization schedule

ELISA testing of bleeds

ELIAS testing for production of KLH antibodies

Post-purification Testing

Reducing and non-reducing gel

Bio-Rad Kit

280/260 UV measurement

Antibody Purifications

Large and Small Scale

DEAE/IgG purification

Protein A/G preparation

Affinity purification

Acid/Base elutions

Column Types

SulfoLink column

Sepharose 4B/BSA column

SulfoLink/BSA/SMCC column

Affi-gel column

Protocol Flexibility

Having the ability to follow the real-time progress of an antibody production provides greater control over the project’s outcome. LifeSpan offers protocol flexibility so that changes can be made quickly to improve the rabbits immune response. Some changes that may effect outcome are:

Changing adjuvant

Double dosing

Variable point of injection

Cost and time savings may also be gained by terminating non-responsive-projects early.

Supporting Products

LifeSpan also offers the following products and services in support of its antibody production service.

Service Deliverables

Upon completion of each antibody project, clients are supplied with affinity-purified antibody, unpurified antisera, peptides, the purification column, and a production report. Depending upon the terms of the agreement, this report will contain an overview of the production and details of the antibody characterization results with accompanying images.

Order an Antibody

To initiate an antibody production project today, or for more information, contact LifeSpan at antibodies@lsbio.com.