RE: [Histonet] Antibody protocol

From:

"Weber, Susan (VHACLE)"

We are using the ultraView DAB from Ventana and the same HSA from Cell
Marque. Here is the protocol we are using. Depar as usual; CC1 Standard;
HSA incubation at 37C for 32 min; Counterstain Heme 4 min; Post
Counterstain Bluing for 4 min. we have found it to be a reliable stain
and very clean and crisp.
Susan M Weber HT(ASCP)
Histology Supervisor
Louis Stokes Cleveland VA Medical Center
10701 East Blvd
Cleveland, Ohio 44106
(216) 791-3800 X6154
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus
Ellin
Sent: Tuesday, November 18, 2008 12:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Antibody protocol
Needing some help with the staining protocol for HSA (Hepatocyte
Specific Antigen) clone OCH1E5, made by Cell marquee to be ran on the
Ventana LT/Benchmark platform.. Would anyone be willing to share
protocol information with us, as well as staining methodology. Thank
you for the help.
Jesus A Ellin HT/PA ASCP
Department of Pathology/Histology
Yuma Regional Medical Center
2400 South Ave A
Yuma, AZ 85364 - 7170
( Office: (928) 336-1743
( Fax: (928) 336-7319
* Email: jellin@yumaregional.org
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