Hi there,
I am trying to measure a fatty acid binding protein in serum.
When I do the purified standard protein dilution in water,
ELISA is very good. The moment I dilute my protein in serum
(immediately before the first incubation), I am not getting any
signal. I do a classical ELISA (coating with protein of interest,
blocking, primary Ab, secondary Ab).
Any ideas on how to try to solve this?
Thanx in advance,
Goran
pavlakovic at gmx.net