Antigen
KLH, isolated from the hemolymphs of the molllusk Megathura crenulata, belongs to a large family of giant respiratory proteins called hemocyanins, which are found in mollusks and arthropods. It is pale blue-gray powder. It contain 0.25% copper. It is supplied in powder in a buffer (5 mM BES, pH 6.5, 10 mM MgSO4). It is soluble in PBS at 5-10 mg/ml.

Expression of genes in E. coli or yeast or baculovirus offers a convenient system to produce large amounts of recombinant proteins that may otherwise be difficult to isolate from natural cells and tissues. Very often antibodies to these newly identified p