Iranian Biomedical Journal مجله بیومدیکال ایرانIBJBasic Scienceshttp://ibj.pasteur.ac.ir1admin1028-852X2008-823X-10.22034/ibj-8888-enjalali139641gregorian2017710online1fulltextenA New Indole Derivative Decreased SALL4 Gene Expression in Acute Promyelocytic Leukemia Cell Line (NB4) Related FieldsRelated Fieldsمقاله کاملFull Length<strong>Background: </strong>Acute myeloblastic leukemia (AML) is a clonal disorder due to bone marrow failure and uncontrolled proliferation of myeloid lineage. Acute promyelocytic leukemia (APL) is a subtype of AML. Heterocyclic compounds, such as indole, are considered as attractive candidates for cancer therapy, due to their abundance in nature and known biological activity. Sal-like protein (SALL4) is a zinc finger transcription factor involving in the multi-potency of stem cells, in the NB4 cell line. This study was aimed to evaluate the effects of basal indole and its new derivative, 2-(1-((2, 4-Aril)imino)-2,2,2-trifluoroethyl) phenyl-1H Indole-3- carbaldehyde (TFPHC), on the expression of <em>SALL4</em>. <strong>Methods:</strong> Cells were cultured and treated with different concentrations (75, 150, and 300 &micro;g/mL) of the new indole derivative and DMSO, as a vehicle control, for 24 and 48 hours. Cell proliferation was evaluated by using Trypan blue exclusion and MTT assays. The percentage of apoptotic cells was&nbsp;determined by flowcytometry analysis using the Annexin V/PI apoptosis detection kit; mRNA expression of <em>SALL4</em> was studied using absolute quantitative RT-PCR. <strong>Results:</strong> Our findings demonstrated the effects of new indole derivatives on <em>SALL4</em> mRNA expression. Expression of <em>SALL4</em> mRNA was significantly decreased at 75, 150, and 300 &micro;g/mL concentrations. <strong>Conclusion:</strong> SALL4 plays a role in the survival of APL cells. <em>SALL4</em> expression could be suppressed by the novel indole derivative. Additionally, <em>SALL4</em> gene suppression can serve as a target in APL therapy.SALL4 protein, Indoles, Leukemia, Acute promyelocytic00http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-685&slc_lang=en&sid=enZahraSheikhrezaei100319475328460030604100319475328460030604Yes
Department of Hematology and Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Kerman University of Medical Sciences, Kerman, IranParisaHeydari100319475328460030605100319475328460030605NoDepartment of Hematology and Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Kerman University of Medical Sciences, Kerman, IranAlirezaFarsinezhad100319475328460030606100319475328460030606NoDepartment of Hematology and Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Kerman University of Medical Sciences, Kerman, IranAhmadFatemi100319475328460030607100319475328460030607NoDepartment of Hematology and Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Kerman University of Medical Sciences, Kerman, IranSoudehKhanamani Falahati-Pour100319475328460030608100319475328460030608NoPistachio Safety Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, IranShokoofehDarakhshan100319475328460030609100319475328460030609NoDepartment of Pediatrics, Rafsanjan University of Medical Sciences, Rafsanjan, IranMojganNoroozi Karimabad100319475328460030610100319475328460030610NoMolecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, IranAliDarekordi100319475328460030611100319475328460030611NoDepartment of Chemistry, Faculty of Science, Vali-E-Asr University of Rafsanjan, Rafsanjan, IranHosseinKhorramdelazad100319475328460030612100319475328460030612NoMolecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, IranGholamhosseinHassanshahi100319475328460030613100319475328460030613NoMolecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran