pCDCAR1 CD33 h(BBζ)(CAR-LC194)

The vector of anti-CD33 chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target human CD33. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-CD33 antibody linked to 4-1BB (CD137) and CD3ζ signaling domains. And the vector product was designed for the treatment of acute lymphoid leukemia.

Details

Target

CD33

Target Species

Human

Targeting Cell Type

T cell

Targeting Diseases

Acute lymphoid leukemia

Generation

Second

Vector Name

pCDCAR1

Vector Length

~8kb

Vector Type

Lentiviral

Receptor Construction

scFv-41BB-CD3ζ

Discription of Signaling Cassetes

41BBCD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. On the basis of preclinical observation, this molecule can promote the persistence of antigen-specific and antigen-nonspecific chimeric antigen receptor T-cells to significantly increases antitumor activity.CD3ζCD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

Antibody Clone

M195

Antibody Host

Mouse

Target

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.