ExiPrep™ Blood Genomic DNA Kit is a product designed to extract high-purity genomic DNA from whole blood samples using the automated nucleic acid extraction instrument ExiPrep™ 16 Plus, ExiProgen™. The lysis buffer and Proteinase K contained within the product will efficiently lysis cells and hydrolysis proteins within whole blood for efficient extraction of genomic DNA. The binding buffer and silica magnetic particles will bind genomic DNA to the surface of the beads. The reaction containers are then subject to a magnetic field, forcing the genomic DNA-bound silica magnetic particles to remain within the container while the reaction mixture and cellular waste are removed. Three types of washing buffers are introduced sequentially during the next step to remove the remainder of cellular waste products. The washing buffers are then siphoned off, while the genomic DNA-bound silica magnetic particles remain bound by the magnetic field. DNase-free elution buffer is then introduced to the silica magnetic particles to release the bound and now purified genomic DNA.

Experimental Data

Fig. 1 Reduced Total Prep. Time
The nano-particles which included in the Buffer ①(Resuspension buffer) can make a complex together with insoluble protein aggregate, cell debris and chromosomal DNA in the solution. And the complex can be separated from the solution with simple centrifuge step(1min.) to obtain a cleared lysate which contains target plasmid DNA.
B; AccuPrep® Nano-Plus Plasmid Mini Extraction Kit, Q, P; Competitors