Figure 3: Particle size of unloaded (a), BSA-loaded (b), and zeta potential of unloaded (c) and BSA-loaded (d) CS/DS nanoparticles in HS and FBS (CS 0.075% w/v, BSA 1 mg/mL, mean ± S.D, ). **Particle size of nanoparticles incubated in HS was significantly different from the ones before incubation and incubated in FBS. Particle surface charge of nanoparticles incubated in HS was significantly different from the ones before incubation and incubated in FBS.