FluoTag®-X4 anti-RFP

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FluoTag®-X4 anti-RFP is a blend of two in-house developed single-domain antibodies (sdAbs) recognizing the most common red fluorescent proteins like mRFP, mCherry, DsRed and other DsRed derivatives with high affinity and specificity. For a complete specificity chart see the Product Specificity Chart. In our FluoTag-X4 series, two fluorophore molecules are site-specifically coupled to each individual FluoTag molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of our FluoTags, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, our FluoTag-X4 series can thus improve the localization accuracy by 10-15 nm. Both features – superior brightness and precise fluorophore placement – render our FluoTag-X4 products excellent tools for all microscopy techniques.

Specificity: Recognizes most common red fluorescent proteins like mRFP, mCherry, dsRed, mScarlet-i and tdTomato in their native conformation. It does not cross-react with GFP or mTagBFP derivatives. For more detailed information see Product Reactivity Chart.Produced in:E.coliConcentration: 2.5 µM protein, 5 µM fluorophoreRecommended dilution for IF/ ICC: 1:250