The cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Cultures can be maintained by addition or replacement of fresh medium. Start new cultures at 4 X 105 viable cells/mL and subculture before the cell density reaches 2 X 106 cells/mL.

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.