{"files"=>["https://ndownloader.figshare.com/files/992454"], "description"=>"<p>A and B, The effect of Six1 on the expression of cyclin D1 protein (A) and mRNA (B) was examined in PANC-1 cells by western blot analyses. GAPDH was used as an internal control. C, cyclin D1 promoter activity was shown to be responsive to increased amounts of a Six1 expression vector, using a luciferase reporter. D, the physical interaction of Six1 to the cyclin D1 promoter in chromatin of PANC-1 cells was shown using a ChIP-qPCR assay. The recruitment of Pol II to the GAPDH proximal promoter was used as a positive control, and the negative control is normal IgG, which controls for the non-specific immunoselection of chromatin by immunoglobulins. Soluble chromatin was prepared and immunoprecipitated with the indicated antibodies. The final DNA extractions were amplified using three pairs of primers as indicated. It showed that Six1 could bind to the −1189 to −985 region of the cyclin D1 promoter but not to the irrelevant −348 to −190 region. All experiments were performed in triplicate; NS, not significant; *<i>P</i><0.05.</p>", "links"=>[], "tags"=>["transcriptionally", "activates", "encoding", "cyclin", "d1", "pancreatic", "cancer"], "article_id"=>656140, "categories"=>["Molecular Biology", "Cancer", "Biochemistry", "Chemistry", "Cell Biology"], "users"=>["Zhaoming Li", "Tian Tian", "Feng Lv", "Yu Chang", "Xinhua Wang", "Lei Zhang", "Xin Li", "Ling Li", "Wang Ma", "Jingjing Wu", "Mingzhi Zhang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0059203.g003", "stats"=>{"downloads"=>3, "page_views"=>21, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Six1_transcriptionally_activates_the_gene_encoding_cyclin_D1_in_pancreatic_cancer_cells_/656140", "title"=>"Six1 transcriptionally activates the gene encoding cyclin D1 in pancreatic cancer cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-21 01:31:29"}