Immunosorbent assay (ELISA) - Essay Example

The search for, determination and detection of antibodies and antigens in an organism’s body can be enhanced by using a technique known as the enzyme-linked immune-sorbent assay (ELISA). ELISA, as a laboratory procedure, determines the absorption of analyte (antibodies or…

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A comprehensive understanding of how an ELISA works would require the determination of what antibodies are and how they work. The basic definition of an antibody is that it is a protein produced by the immune structure in reaction to the foreign molecules in an organism’s body.
Under circumstances that require the attainment of indirect semi-quantitative results, multiwall polystyrene wells are the most commonly used solid surface. ELISA, being quick and easy to undertake, is preferable given that its makeup also enables it to tackle voluminous samples consecutively during research and diagnostic targets. As a result, ELISA is considered the best choice when evaluating research and diagnostic targets (Killian et al., 2013). The process of performing ELISA depends on the relations between an epitope; it may be a small linear or a three-dimensional succession of amino acids established on an antigen and a corresponding antibody-binding site.
This experiment aimed at determining how the concentration of antibodies in the body would be affected by the use of ELISA under indirect semi-quantitative and anti-human albumin antibody. This is because of the wide utilization or applications to which the enzyme can be put such as in the analytical implements in biomedical research to detect and quantify specific antibodies and antigens in a sample. In this experiment, the antibodies employed were either monoclonal or polyclonal (Rosie, 2013). When performing any ELISA test, there are four formats of performing the laboratory tests: direct ELISA, indirect ELISA, sandwich/capture ELISA, and competitive or inhibitor ELISA. These allows the experiment’s elasticity to fit basing on antibodies available, the needed results and complications of the essential results (Rodland, 2005.p.145-148). In this experiment, the variation employed is the indirect ELISA, which employs the human albumin ELISA test.
Given that ELISA test utilizes the rudimental concept
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?.10 Figure4: Principles of immunohistochemistry………………………………………………12 Figure 5: Steps involved in western blotting………………………………………………..14 The Rationale behind Antibody Usage in Common Laboratory Assays Such As ELISA, Immunofluorescence, Immunohistochemistry, and Western Blotting, their Relative Advantages and Disadvantages Introduction The immune system produces antibodies in reaction to an assortment of foreign substances as well as infections in the body.

There are also other plasmodium species which cause malaria. These are plasmodium ovale, plasmodium malariae and plasmodium vivax which are considered as less dangerous species. Malaria is spread by the female anopheles mosquito which while feeding on human blood injects its saliva into the body.

The diluted serum suspected to be containing specific immunoglobulin antibody for bound antigen is added to the ELISA plate/ well and allowed time to react. Enzyme labeled immunoglobulin is then added, allowed time for

It is important to use multichannel pipette to avoid any manual error at a time of filling reagents to microtiter plates. Similarly, to avoid error like at concentration of 2.0 there must be at least 3 replicates which make data more meaning full and realistic in terms of statistic.

The reason for which ELISA is favoured foe biological tests is that its reactions can be measured both qualitatively and quantitatively. Consequent to this advantage, ELISA is used in biological and medical researches and in clinical setting for tests and diagnoses (Icon Group International, 2011).

common representative of Chlamydia genus is Chlamydia trachomatis which can cause Chlamydia infection – an infectious disease that is transmitted through sex. It is one of the most common sexually transmitted disease. Infection with chlamydia usually transfers through sex,

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