The train doesnt come into view until about 6:35 or so. In regards to lookin like Astrotrain I just don't see it. It's a locomotive, but I think that's where the similarities stop. Mind you it is possible. Here's two Transformers references I can recall off the top of my head:

The Simpsons - Aside from the latest Halloween special there was a couch gag where the family members were versiosn of jetfire, prime, bumblebee, seaspray, and arcee. I think it was them...

Grim Adventures of Billy & Mandy - The episode with the wishing skull finds Pud'n being chased down with an Optimus Prime lookin semi driven by an evil tough-lovin teddy bear.