BDNF IHC

From:

Sandra Uhl-Bronner

Please, send your response(s) to :
sarhan@neurochem.u-strasbg.fr

Hello,
I'm trying to do bdnf immunohistochemistry on the rat brain but i've only
had non specific labelling with the protocols i've used.
I'm using the chemicon sheep anti-bdnf polyclonal antibody and i've tried
different protocols incuding
formol 4% piciric acid 0,2%
formol 2% parabenzoquinone 0,2%
with or without ethanol washes, vibratome or cryostat sections.
i'm not very used to bdnf or even growth factors in general ; it seems
that bdnf when associated to its trk receptor would be masked for
localization.
does anyone have any experience in that field and could help me find out
the right way to find specific bndf labelling with my antibody or any
other commercial antibody ?
Thank you in advance !
SARHAN Maysa