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Our Cas9-sgRNA plasmids contain both the Cas9 gene and an sgRNA gene. The inclusion of both components on a single plasmid facilitates archival and distribution, and makes it simpler to prepare injection mixes. The Cas9 coding sequence is codon-optimized and includes synthetic introns for efficient expression in C. elegans , and is driven by the eft-3 promoter for germline expression. The sgRNA is driven by the R07E5.16 U6 RNA promoter. Compared to the C. elegans CRISPR plasmids from John Calarco's lab, which are also available on Addgene, the biggest differences are 1) Inclusion of both components on the same plasmid, and 2) Use of a different U6 promoter to drive sgRNA expression. We have not compared the two systems side by side but would expect them to be similar in terms of efficiency.