This study assesses the assimilation of knowledge and preventive practices in oral health among the mothers of children assisted by a mother-child dental care program. The Preventive Program for Pregnant Mothers and Babies (PPPMB) is an extension project run by the Piauí Federal University (UFPI), whose goal is to make pregnant women and mothers of children from zero to 36 months old more concerned about habits fostering oral health. After a random selection of clinical record cards for children who had participated in this Program, letters were sent to their mothers. Feedback was received from 281 mothers, who responded through interviews that included questions on matters addressed by the Program. The findings indicate that mothers attending this Program were properly informed, adopting hygienic practices in their families that underpin the control and prevention of oral diseases.