GenElute™ Agarose Spin Columns

Procedure: A slice of agarose gel containing the DNA of interest is excised, placed into a spin column, and spun at maximum speed in a microcentrifuge. Agarose is retained on a filter in the spin column. Soluble molecules, including DNA, pass through the filter and collect in a microcentrifuge tube (Figure 1).

Application: DNA can be used in ligation, restriction enzyme digestion, cloning and PCR

Beneficial Features

Starting Amount

< 200 mg gel slice

Yield

Recovers up to 40 to 45% of DNA fragments from 100 bp to 10 kb

Speed

One-step, 10 minute protocol

Flexible

DNA concentrated in 50 µL to 200 µL of buffer depending on size of gel slice

Left Panel: 1 µg of 100 bp DNA ladder was electrophoresed on a 2.0% agarose gel. 100, 200, 300, 400, 500, and 600 bp fragments were excised from the gel, and purified using GenElute Agarose Spin Columns as above. Samples were loaded and analyzed on a 2.0% agarose gel.

Right Panel: Lambda Hind III markers were pre-heated at 65°C and electrophoresed on a 2.0% agarose gel. The 2.0, 4.4, 6.6, 9.4, and 23 kb fragments were excised and purified using GenElute Agarose Spin Columns. Samples were loaded and analyzed on a 2.0% agarose gel.