For day-to-day checks on reproducibility and wavelength calibration a set of six PMMA (polymethyl-methacrylate) blocks can be used. These have emission characteristics in the following wavelength ranges:

Wavelength Range (nm)

1. 300 – 400 (anthracene/naphthalene)

2. 420 – 600 (ovalene)

3. 300 – 400 (p-terphenyl)

4. 400 – 600 (tetraphenyl/butadiene)

5. 600 – 640 (europium)

6. 540 – 640 (rhodamine B)

For checking instrument sensitivity, a 10 mm sealed cell contains high-purity water, which acts as a constant sample to reproducibly measure signal-to-noise using the Raman band.