Abstract

Introduction

Over-expression of anti-apoptotic proteins such as X-linked inhibitor of apoptotic protein (XIAP) leads to defects in caspase-3 mediated apoptosis regulation and is one of the main causes of cancer development and is associated with poor clinical outcome. Targeting of XIAP in conventional cancer therapy has the potency to significantly improve success rates of cancer treatment and the survival of patients. Our study is a preliminary effort to study the gene expression of less studied XIAP and caspase -3 in oral cancers by amplification of these genes through PCR method and 8-oxodG, a marker of oxidative damage.

Material and Methods

This Prospective pilot study is based upon the expression of XIAP and caspase protein among patients with oral cancer. PCR- gel electrophoresis was performed to analyze the gene expression and level 8-oxo dG was measured by performing ELISA.

Results

Conclusions

We have optimized the protocol for the molecular analysis of these genes through PCR and propose to continue the study on a larger sample size with suitable controls and also include real time PCR and Western blot techniques for evaluating the expression of XIAP protein and caspase -3 and its role in future target therapy in oral cancer patients.