Western blot：The antibody, when used at 1μg/ml concentration, identified the 100KDa PSMA in LNcap cell extract. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form

Liquid

Purification

Protein G affinity purified

Buffer

0.01M PBS (pH 7.0)

Concentration

1 mg/ml

Storage instruction

For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Prostate specific membrane antigen (PSMA) is a type II trans-membrane glycoprotein found in the epithelium of prostate. It is also detectable in duodenal mucosa, renal tubules and some neuroendocrine cells. In other normal tissue, PSMA expression is not detectable. Because the expression of this membrane is elevated in malignant prostate tumour, PSMA has been used as a marker as well as a therapeutic target for prostate cancer. Elevated PSMA expression has also been identified in metastases of lymph nodes.

Function

Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC. [UniProt]

Resrarch Area

Cancer antibody; Metabolism antibody; Signaling Transduction antibody

PTM

The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.The N-terminus is blocked.