If you are in a hurry and your selection marker is ampicillin, you can go ahead and plate immediately because ampicillin takes a while to be pumped into cells at a high enough concentration to have an effect.

If you are in a hurry and your selection marker is ampicillin, you can go ahead and plate immediately because ampicillin takes a while to be pumped into cells at a high enough concentration to have an effect.

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[[Category:E.Coli Protocol]]

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[[Category:Protocol]]

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[[Category:In vivo]]

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[[Category:Escherichia coli]]

Current revision

This protocol is for transforming plasmid DNA into Escherichia coli cells.

If you don't have any transformants, plate the rest of the transformation in the morning.

Notes

If you are in a hurry and your selection marker is ampicillin, you can go ahead and plate immediately because ampicillin takes a while to be pumped into cells at a high enough concentration to have an effect.