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RNAscope® Technology

Read about reseachers who take innovative approaches with RNAscope® protocols to advance their research. If you would like to try one of these customer-demonstrated applications, please note that they are non-standard applications and therefore, a careful read through of the publications/materials is highly recommended. Our technical support team is here and will make best efforts to support you.

Innovations in the Spotlight:

You may be surprised to know that your archived FFPE samples may not be too old for RNAscope®in situ hybridization (ISH)! Our R&D team routinely detects RNA transcripts in three year old FFPE tissue specimens and some of our customers have applied RNAscope to much older tissues. Read below, data from one of our customers where RNAscope ISH was successfully used in tissue specimens older than 25 years.

Researchers Marije Hoogland and Esther Verhoef (Department of Pathology, Erasmus MC Rotterdam, the Netherlands) have conducted a retrospective study and successfully performed RNAscope®in situ hybridization on samples containing human metastases of prostate cancer in a lymph node. The samples were collected in 1987, 1988 and 1989 and analyzed in 2014 with RNAscope® probe targeting UBC gene. At 25 to 27 years old, these are the oldest samples we know of that have been used with RNAscope®in situ hybridization. The team followed ACD protocols and user manuals.

FAQs for RNAscope® Assay on archived FFPE samples

How can I ensure that my FFPE tissue specimens will work with RNAscope® FFPE Assay?

We have conducted extensive assay development to make sure that the RNAscope® FFPE assay will work with a wide range of tissue types.

If your tissue specimens are processed according to the Sample Preparation Guide in the User Manual and they are prepared within the last 3 years, you should expect successful staining for positive control probe using our assay procedure.

If your specimens are prepared differently from our recommended procedure, RNAscope® can still be used but may require assay optimization for some procedural steps. Please refer to our getting started page for protocol and sample guidelines and optimization.

Ensure that the assay is run according to RNAscope® protocol.

What are the recommended RNAscope® sample storage and pretreatment conditions for FFPE samples?