Dear Jill,
For my senior year research, I was tracking the location of carbonic
anhydrase during sclerite formation in a species of octocoral found here
along the East and Gulf coasts of the US. I used rabbit antibodies made
against the CA of our little "critter" and then labeled with gold-tagged
goat anti-rabbit antibodies. Then I post-stained with osmium. The TEM
images were lovely after the post-staining. They looked just like they had
been prepared for structural observation rather than antibody labeling. I
had quite a time with high backgrounds at first, but was able to solve that
problem by using Tween 20 and immunoprecipitating my primary antibody using
Staph A. If any of this sounds interesting to you, let me know and I will
send you what I have.
Regards,
Beth Bray
bbray@netside.com