Antigen Retrieval Solutions

HIER Buffer

These buffers unmask epitopes that have been cross-linked by formalin fixation. These solutions are specially formulated for superior pH stability at high temperatures. All Decloaker solutions incorporate Assure™ color-coded pH indicator technology, this assures by visual inspection that the solution is at the correct dilution and pH. Products can be stored at room temperature, with the exception of EDTA. All buffers are sodium azide and thimerosal free. These products are specially formulated for superior pH stability at high temperature, and will help prevent the loss of pH-sensitive antigens. The retrieval solutions also block endogenous peroxidase and reduce nonspecific background, except Antigen Decloaker, Nuclear Decloaker and EDTA Decloaker.

Assure™ pH Color Indicator

Superior Staining

Non-hazardous Solutions

Ki-67 stain using Biocare’s Diva Decloaker buffer for HIER

Diva Decloaker – Preferred HIER for Human Tissue

Eliminates the need for multiple buffers including citrate, EDTA or high pH Tris. Antibody titers are doubled and tripled when compared to citrate buffer. Diva Decloaker is non-toxic, non-flammable and odorless, pH 6.2.

Rodent Decloaker – Preferred HIER for Mouse and Rat Tissue

A Novel Heat-Induced Epitope Retrieval (HIER) Solution for Rodent Tissues
Used for the unmasking of epitopes cross-linked by formalin fixation and blocking endogenous mouse and rat IgG. It has been specially formulated for use on mouse and rat tissues with emphasis on rat brain. Rodent Decloaker has been formulated to work with Biocare’s Promark series of mouse, rat, goat and rabbit polymer detection kits. It is designed for research use only, and not intended for therapeutic and diagnostic purposes.

Reveal Decloaker

Reveal is a specially formulated heat-retrieval solution. It is used in conjunction with Biocare’s Decloaking Chamber Pro. Reveal is non-toxic, non-flammable and odorless. Antibody titers are increased compared to citrate buffer, pH 6.0, and background staining is reduced significantly. Reveal also blocks endogenous peroxidase and mercury crystals.

Borg Decloaker

Borg Decloaker is a Tris-Based formulation, pH 9.5, and is non-toxic, non-flammable and odorless. Tissues can be deparaffinized and then slides can be placed into the Borg Decloaker used in conjunction with Biocare’s Decloaking Chamber or other heating methods. In most cases, antibody titers will increase compared to other heat-retrieval buffers.

Bull’s Eye Decloaker, 20X

Bull’s Eye is a 20X buffer heat retrieval solution with color-coded pH indicator that allows the end-user to block for endogenous peroxidase, block for non-specific background staining and antigen retrieve tissues simultaneously. Bull’s Eye is a universal buffer and is compatible with virtually all antibodies and thus eliminates the need for EDTA or high pH retrieval buffers.

EDTA Decloaker

An EDTA buffer formulation with a pH range of 8.5 (after dilution), with preservative. This product is specially formulated for superior pH stability at high temperature, and will help prevent the loss of pH-sensitive antigens.

Antigen Decloaker

A specially formulated citrate buffer, with preservative, designed for superior pH stability at high-temperature incubations. The pH 6.0 formulation helps prevent the loss of pH-sensitive antigens.

Nuclear Decloaker

This is a Tris buffer formulation, pH 9.5 with preservative. Specially formulated for superior pH stability at high temperature, this product will help prevent the loss of pH-sensitive antigens.

Antigen Retrieval Solutions Reference Chart

Concentrate is diluted in DI water; non-flammable and non-toxic; no deparaffinization

Rodent Decloaker

Heat retrieval only

(Proprietary)

6.0

10X

Diluted in DI water; non-flammable and non-toxic; no deparaffinization

Reveal

Heat retrieval only

Citrate-based

6.0

RTU / 10X

Concentrate is diluted in DI water; non-flammable and non-toxic; capable of deparaffinizing, but if used for this purpose, must be used in conjunction with Hot Rinse

Borg Decloaker

Heat retrieval only

Tris-based

9.5

RTU

Used after deparaffinization; non-flammable and non-toxic; capable of deparaffinizing, but if used for this purpose, must be used in conjunction with Hot Rinse

Universal Decloaker

Heat retrieval only

(Proprietary)

6.0

10X

Diluted in DI water; non-flammable and non-toxic; no deparaffinization

Antigen Decloaker

Heat retrieval only

Citrate-based

6.0

10X

Concentrate is diluted in DI water; non-flammable and non-toxic; no deparaffinization

Bull’s Eye Decloaker

Heat retrieval only

(Proprietary)

6.0

20X

EDTA Decloaker

Heat retrieval only

EDTA-based

8.5

5x

Diluted in DI water; non-flammable and non-toxic; no deparaffinization

Nuclear Decloaker

Heat retrieval only

Tris-based

9.5

10X

Concentrate is diluted in DI water; non-flammable and non-toxic; no deparaffinization

Reveal and Borg HIER solutions may be used in a two-step deparaffinization and antigen retrieval in combination with Biocare’s Hot Rinse product.

DepART Solution

DepART Solution is a water-soluble deparaffinization reagent that can be used for immunohistochemistry (IHC) and H&E. Unlike other commercially available watersoluble reagents, paraffin is completely removed by this non-flammable solution. DepART Solution eliminates the use of xylenes and alcohols. DepART Solution can be used as part of a two-step deparaffinization retrieval protocol with most retrieval solutions, including Biocare’s Reveal, Diva or Borg. Biocare Medical has performed in-house studies comparing DepART Solution with xylene/alcohol reagents and found equivalent staining results for both IHC and H&E. The DepART Solution can be reused up to 10 times, for a total of 100-200 slides over a period of up to two weeks. Store solution covered, at room temperature between runs.

Hot Rinse

Clarifying reagent to be used with Reveal or Borg HIER reagents.

Aqua DePar

Aqua DePar is a water-soluble deparaffinization reagent which can be used for IHC, H&Es, and special stains. Paraffin is completely dissolved by this non-flammable and non-hazardous, safe-to-use solution. Eliminates the use of xylenes and alcohols, reducing disposal costs and toxic waste. Aqua DePar can be used with a two-step deparaffinization retrieval protocol using Reveal or Borg HIER reagents.

Diluted in DI water, then heated to 65-75° C and used only for deparaffinization; non-flammable* and non-toxic**

HIER Reference Chart

HIER

Intended Use

Composition

pH

Formats

Usage

Borg Decloaker***

Heat retrieval only

Tris-based

9.5

RTU

Used after deparaffinization; non-flammable* and non-toxic**; capable of deparaffinizing, but if used for this purpose, must be used in conjunction with Aqua DePar

Reveal Decloaker***

Heat retrieval only

Citrate-based

6.2

RTU & 10X

Concentrate is diluted in DI water; non-flammable* and non-toxic**; capable of deparaffinizing, but if used for this purpose, must be used in conjunction with Aqua DePar

Slide Brite™

Slide Brite is a non-hazardous alternative to xylene for the deparaffinization and clearing of tissue sections. The flash point for this reagent is above 140°F (60°C), double that of xylene. Slide Brite has been designated non-hazardous on the basis of aquatic toxicity, and requires no hood or ventilation.

All prices presented are US list price for sale to clients in the Domestic US only. Please see your international distributor for country specific pricing. For a full list of international distributors, click here.