In enzymology, a maltose phosphorylase (EC 2.4.1.8) is an enzyme that catalyzes the chemical reaction

maltose + phosphate D-glucose + beta-D-glucose 1-phosphate

Thus, the two substrates of this enzyme are maltose and phosphate, whereas its two products are D-glucose and beta-D-glucose 1-phosphate.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is maltose:phosphate 1-beta-D-glucosyltransferase. This enzyme participates in starch and sucrose metabolism.

ELISA: 1:2000-1:10,000; 1ug of Maltose Phosphorylase was used in a standard sandwich ELISA with Rabbit anti-Goat (HRP) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]), A0030, as a substrate for 30 minutes at RT.

Optimal dilutions to be determined by the researcher.

*Recommended Secondary Antibodies

I1904-28K: IgG, H&L (HRP) Pab RbxGt

I1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab RbxGt

Storage and Stability

May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.