TaqMan Master Mix: Premix Ex Taq (Probe qPCR)

The Takara Bio Premix Ex Taq (Probe qPCR) is a 2X TaqMan Master Mix for real-time PCR (qPCR) using TaqMan® probe detection. The 2X TaqMan Master Mix includes TaKaRa Ex Taq HS, a hot start PCR enzyme in combination with anti-Taq antibody, in a qPCR optimized buffer. TaKaRa Ex Taq HS inhibits non-specific amplification while enabling high efficiency amplification and detection sensitivity during real-time PCR analyses. Additionally, Tli RNase H, a heat-resistant RNase enzyme, is included in the real time PCR premix in order to minimize PCR inhibition due to the presence of residual mRNA in the input cDNA. This TaqMan Master Mix product is ideal for high-speed PCR, allows accurate target quantification and detection over a broad dynamic range, and enables highly reproducible and reliable real-time PCR analyses.

Two tubes of ROX Reference Dye are supplied separately with Cat. #RR390A/B and RR390L/W. The ROX Dye serves as a convenient internal reference standard when normalizing signals resulting from non-PCR fluorescence fluctuations that occur between wells or over time in different instruments.

Applications

Components

RR390A (200 reactions (50 µL rxns); 500 reactions (20µL rxns))

Premix Ex Taq (Probe qPCR) (2X conc.)*1

5 X 1 mL

ROX Reference Dye (50X conc.)*2

200 µL

ROX Reference Dye II (50X conc.)*2

200 µL

1: Contains TaKaRa Ex Taq HS, dNTP Mixture, Mg2+ and Tli RNase H

2: Use when performing analyses with real-time PCR instruments (e.g., Life Technologies) that normalize fluorescent signals between wells. Use ROX Reference Dye for ABI PRISM® 7000/7700, 7300 Real-Time PCR System and StepOnePlus™ Real-Time PCR System. Use ROX Reference Dye II for 7500 Real-Time PCR System and 7500 Fast Real-Time PCR System. Use the ROX Reference Dye at a final concentration of 1X and the ROX Reference Dye II at a final concentration of 0.5X. No Reference Dye is required when using Thermal Cycler Dice® Real Time System II , SmartCycler® System or LightCycler®.