OECD 302B – Inherent Biodegradation

OECD 302B is an inherent aerobic biodegradation test used for determining the biodegradability of a solution typically not readily biodegradable, is known to be insoluble or does not satisfy the requirements of OECD 301.

For OECD 302B, the determination of Dissolved Organic Carbon (DOC) dominantly monitors the biodegradation process; however, Chemical Oxygen Demand (COD) can also be used. Standard testing for OECD 302B is 28 days; however, as an inherent biodegradability test, testing can be extended up to 60 days.

Inherent biodegradability testing is commonly requested for test materials that most likely or knowingly will now pass the 301 ready biodegradability requirements. The additional time for testing allows for the inherent biodegradability of the material to be evaluated.

When performing a biodegradation test, it is important to communicate different properties of the material with the testing laboratory, including water solubility (OECD 105), volatility, and vapor pressure. There are a variety of chemical and physical properties that can affect biodegradability results; for more information on biodegradation testing and chemical and physical properties, please visit our Biodegradability Testing – Chemical Impacts page.

Biodegradation testing is commonly required for many label claims regarding a material’s biodegradability performance. For example, the FTC commonly regulates these claims; however, it is strongly recommended to review testing requirements with the appropriate regulatory agency prior to testing to determine which biodegradation test is required to make performance claims about a material’s biodegradability.