EGFR 18-21 MASTR - Multiplicom

The EGFR MASTR assay amplifies exons 18 to 21 of the EGFR gene and consists of 4 amplicons in a single multiplex PCR reaction. Amplicon sizes range from 100 bp to 180 bp enabling mutation detection in FFPE derived DNA.

The Epidermal Growth Factor Receptor (EGFR) is a cellular transmembrane receptor found on the cell surface of tumour cells. It is activated by the binding of the EGF or other growth factors which finally stimulates cell proliferation. Mutations in the tyrosine kinase domain, comprised by exons 18-21, of the EGFR gene increase the activity of the intracellular signaling cascade resulting in uncontrolled proliferation of the tumor cells, such as non-small cell lung cancer (NSCLC). Identifying the TK domain mutations is applied in guiding targetted therapy strategies with tyrosine kinase inhibitors.