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Description

Applications

Amphotericin B is used as antifungal in cell culture media.

Product Description

Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. It has a high affinity for sterols, primarily ergosterols, of fungal and bacterial cell membranes. It is an antibiotic solution effective against yeast and other fungi. Amphotericin B may be toxic to some insect cell types. Amphotericin B, is an antimycotic.

Biochem/physiol Actions

It interrupts cell membrane permeability resulting in the loss of low molecular weight compounds from the cell. Polyene antifungal antibiotic from Streptomyces. Affinity for sterols, primarily ergosterols, of fungal cell membranes. Forms channels in the membranes, causing small molecules to leak out. Amphotericin B induces K+ leakage, which is separate from its lethal action, as was demonstrated in human erythrocytes and is due to the inhibitory effect on the Na+/K+pump. At sub-lethal concentrations, this drug stimulates either the activity of some membrane enzymes or cellular metabolism, in particular stimulation of some cells of the immune system.

Recommended Use

To kill yeast and fungi, it is recommended to use this product at 2-4 times the normal level (20-40 ml/L) in medium without penicillin and streptomycin, for 2-3 subcultures. Recommended for antifungal use in cell culture media at 2.5 mg/L.

It is formulated with deoxycholate and prepared in water for increased solubility.

pKa

5.5, 10.0 (Lit.)

Foreign activity

Mycoplasma free.

Solubility

Slightly soluble in water; DMF/1M HCL 3/1: 49.00 - 51.00 mg/mL; Amphotericin B is insoluble in water at pH 6 to 7, but soluble in water at pH 2 or 11. It is soluble in DMSO (30 – 40 mg/mL) and in dimethylformamide (2 – 4 mg/mL).

Kosei Ando Effect of dynamic compressive loading and its combination with a growth factor on the chondrocytic phenotype of 3-dimensional scaffold-embedded chondrocytes. Acta Orthopaedica200980, 724-733