Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Purification methods range from very crude to highly specific. The level of purification necessary to obtain usable antibody depends upon the intended application(s) for the antibody. Antibodies may be purified by class (e.g., IgG) without regard to antigen specificity, or affinity purified to an immobilized antigen that is antibody specific.

We offer a wide selection of ligands and formats to purify total or specific antibodies from serum, ascites fluid, or tissue culture supernatant. Our portfolio is designed to meet small-scale (screening) to large-scale (bioprocess) needs.

Featured antibody purification categories

Protein A binds to human, mouse, goat, and rabbit IgG, (except for human IgG3 and mouse IgG1.) at the Fc portion of the intact Ig and is typically the ligand of choice when purifying IgG from rabbit polyclonal antisera.

Protein A/G is a genetically engineered protein with combined IgG binding profiles of both Protein A and G. It contains four Fc binding domains from Protein A and two from Protein G, providing additive class-specific binding properties for Protein A and G.

Protein L binds specifically to Ig kappa light chains, and members of all antibody classes (IgG, IgM, IgA, IgD, and IgE). It is the affinity molecule of choice for the positive selection of kappa Fab fragments or recombinant single-chain variable fragments.

Partner with us to scale-up your process or product development pipeline with bulk quantities of high-quality immunoassay reagents and biochemicals. We offer a variety of compounds, devices, and resins for your large-scale applications.

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