The road forms a part of the Prospect Highway, which is itself an important secondary road linking the Hills District to the M4 Motorway. The road runs from Blacktown's Main Street to the Great Western Highway. On the other side of the Great Western Highway it becomes Clunies Ross Street.

Blacktown Hospital, as well as several home improvement retail-warehouse complexes, are located along Blacktown Road. Several Busways and bus routes operate along Blacktown Road, providing access to Blacktown Hospital, Blacktown TAFE and Blacktown railway station.