Sample_source_name_ch1: RNA purified from control cells_polysomal fraction of polyribosomes

Sample_organism_ch1: Saccharomyces cerevisiae

Sample_characteristics_ch1: strain BY4741

Sample_treatment_protocol_ch1: 100 mL cultures were grown to OD600nm 0.5, when DTT was added to a final concentration of 2mM (or an equivalent volume of water was added to control cultures), and flasks were returned to the incubator for a further 1 hour. Cyclohexomide was added to a final concentration of 0.1mg/mL, and flasks were cooled immediately on ice.

Sample_label_protocol_ch1: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 μg total RNA (Affymetrix Manual II).

Sample_hyb_protocol: Following fragmentation, 20 μg of cRNA were hybridized for 16 hr at 45oC on high-density oligonucleotide Yeast Genome S98 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.

Sample_scan_protocol: GeneChips were scanned using the Affymetrix GeneArray 3000 Scanner, according to the Affymetrix Manual II.

Sample_description: Polyribosomes following DTT or control treatment

Sample_data_processing: The data were analysed with Affymetrix CGOS software. The average intensity of all probe seta was used for normalisation and scaled to 100 in the absolute analysis for each probe array.