Separation of glycerol from protein

It could be a very stupid question, but just came in to my mind and thought of asking the best people in research . For storage of protein in -80, we add some % of glycerol in, so that the ice crystal could not destroy our protein. I was wondering, if it is possible to remove glycerol from protein by dialysis or some other method??

Then I thought people do sucrose gradient or glycerol gradient for various proteins separation. After visualizing on gel, do they separate protein from sucrose or glycerol?

Thanks bob . Same here, I also don't work on these stuffs. But I was thinking if people do that? I tried to google also but it dint help. Like in density gradient experiments, I've not seen people purifying the protein or that component from the fractions.

I agree with the ease of the spin filters. Using them for buffer exchange is limited to protein solutions that are either dilute or that can handle be concentrated to the extent necessary to acheive 'buffer exchanged' status. If you don't need concentration, the gel filtration columns are just as easy and more gentle on your protein. It's good to identify how much buffer exchange is necessary for your application and then calculate how much buffer exchange you've acheived with either the spin filter or the gel filtration columns. I've had to buffer exchange things twice in order to get efficient conjugation.