This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognises both dimethyl and monomethyl arginine it can be used in parallel with ab413 (detects dimethyl arginine only) and ab414 (detects monomethyl arginine only) to monitor the exact modification status. A tissue culture supernatant version is available as ab5394.

製品の特性

製品の状態Liquid

保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

バッファーConstituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose

Concentration information loading...

精製度Protein A purified

一次抗体 備考This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognises both dimethyl and monomethyl arginine it can be used in parallel with ab413 (detects dimethyl arginine only) and ab414 (detects monomethyl arginine only) to monitor the exact modification status. A tissue culture supernatant version is available as ab5394.

Anti-mono and dimethyl Arginine antibody [7E6] 画像

Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure (see protocol) with ab412 to mono and dimethyl Arginine.

Immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 3 µl of ab412 and 4x106 cells were used in each ChIP experiment.

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Thank you for contacting us. Your credit note ID is *.I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasin...

Thank you for your reply.I am sorry that the antibody is not working for you an based on the information that you have provided, I do not believe that there is ay protocol advice that I can offer that would resolve the issue.Therefore I would...

Thank you for contacting Abcam.The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on a variety of samples . If we cannot resolve the issue you are having with the antibody then I would be happy to ...