Summary

CHDR has extensive experience with the isolation of primary human cells for downstream analysis of drug effects. Since sample handling can largely affect cellular behavior, optimization of cell isolation and storage is an important point of attention. For example, CHDR has three different standardized procedures in place for isolation of peripheral mononuclear blood cells, and selects one of these three for application in a clinical study, based on the intended use of the isolated cells. The availability of a flow cytometer and well-trained technical staff allows quality control of isolated cells, in terms of yield, purity and viability. Moreover, CHDR’s in-house laboratory is equipped with Stemcell’s RoboSep magnetic cell separator. Cell sorting is frequently applied in CHDR’s clinical studies for focused analysis of drug effects on target cells

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