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Anthony Hancock arrives at his local hospital to give blood. "It was either that or join the Young Conservatives", he tells the nurse (Whitfield), before getting into an argument with her about whether British blood is superior to other types. After managing to offend some of the other waiting donors he amuses himself by reading the waiting room's wall posters out loud, finally singing "Coughs and sneezes spread diseases" to the tune of the German national anthem before being shown in to see the doctor (Cargill).

After the doctor has taken a blood sample Hancock blithely assumes that that is all that is needed and prepares to depart. When the doctor tells him it was only a smear and that he must donate a pint of blood, he protests, "I don't mind giving a reasonable amount, but a pint! That's very nearly an armful!" The doctor finally persuades Hancock to donate the full pint by telling him he has a rare blood type, which appeals to Hancock's snobbery. Having boasted of his lack of squeamishness, he faints while giving blood. Recuperating afterwards, Hancock has a chat about blood with a fellow patient (Lloyd), but since neither of them knows very much about blood the conversation is not very informative. As he prepares to leave Hancock is horrified to discover that the other patient has stolen his wine gums. ("If you can't trust a blood donor, who can you trust?")

After returning home, Hancock cuts himself on a bread knife and is rushed back to the same hospital, where he receives a transfusion of his own blood—the only pint the hospital has of his rare blood type.

While returning from recording The Bowmans a week earlier, Hancock was involved in a car accident, and rather than cancel the studio booking it was decided to place teleprompters around the set in order to save the comedian the problems of learning his lines after a delay in rehearsals. In the passage where he is alone looking at the posters, Hancock was obliged to learn his lines. While it is not true Hancock never learned a line from a script again, he increasingly relied on the device mostly thereafter in his TV career. Many Hancock devotees reckon this is one of his poorest TV performances, and it is indeed quite noticeable to see Hancock's eye movements towards the teleprompters when addressing the other performers. Others, though, see no real difference between this and other classic Hancock performances, and lines from the episode have become a part of British comic folklore ("A pint? That's very nearly an armful!" "Rhesus? They're monkeys aren't they?"), giving it now-legendary status.

After "The Blood Donor", Hancock made one last episode for the BBC ("The Succession—Son and Heir"), before leaving the corporation somewhat acrimoniously, but more importantly severing his professional relationship with his writers, Galton and Simpson. His career never recovered from these decisions.

In October 1961 Pye Records produced an audio remake of "The Blood Donor" (duration: 28'10") starring most of the original cast, together with a remake of "The Radio Ham" (duration: 27'40") from the same series. These were produced in the style of the radio shows, complete with an (invited) studio audience, and released as an LP in 1961.[1] These recordings have been available more or less continuously ever since, and are also found on several British comedy compilation sets.

The original episode survives in the BBC Archive as a 16mmtelerecording with a separate magnetic soundtrack, and has been released on Laser Disc (BBCL 7004), VHS (BBCV 7034) and Betamax (BBCB 7034) in 1985 and DVD (2000) compilations of Hancock episodes. The original soundtrack from the episode was also subsequently released on CD and audio cassette.

The script was re-recorded in 2009 for a BBC Radio 2 series of remakes of Galton and Simpson works. "The Blood Donor" starred Paul Merton, with Suzy Aitchison as the nurse, the role played by her mother 48 years earlier. It was first transmitted in March 2009.[2][3]