Immunocytochemistry (1-10µg/ml)Immunoprecipitation (1-4µg per sample)Western Blot (1-5µg/ml)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Application Notes:

Detects bands of ~95kDa (Ski) and bands of up to ~115kDa (hyperphosphorylated Ski) by Western blot.

Purity Detail:

Protein G-affinity purified.

Formulation:

Liquid. In PBS containing 0.05% sodium azide.

Shipping:

Shipped on Dry Ice

Long Term Storage:

-20°C

Scientific Background:

Ski (v-ski) was discovered as a viral oncogene from the genome of avian Sloan-Kettering retrovirus. Overexpression of both v-ski and cellular homolog c-ski in avian fibroblasts leads to anchorage-independent growth and morphological transformation of chicken embryo fibroblasts. Overexpression of c-ski has also been observed in several types of human cancer cells. It has been shown that Ski associates with Smads2, 3 and 4 proteins in response to TGF-β stimulation. Interactions between Ski and Smad2, 3 and 4 blocks Smads-mediated transcriptional activation as well as TGF-β-induced growth suppression of epithelial cells, suggesting oncogenic effects of Ski derive in part through its ability to antagonize the growth-inhibitory signals initiated by members of the TGF-β family of ligands. Ski is also required for transcriptional repression mediated by Mad involved in negative regulation of cellular proliferation and plays a role in muscle differentiation.