Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat ALPI concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ALPI. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ALPI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ALPI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ALPI. You can calculate the concentration of Rat ALPI in the samples by comparing the OD of the samples to the standard curve.

Assay type

Sandwich

Format

96T

Assay time

4.5h

Reactivity

Rat

Detection Method

Colormetric

Detection Range

0.78—50 ng/mL

Sensitivity

0.47 ng/mL

Sample Volume Required Per Well

100 μL

Sample Type

Serum, plasma and other biological fluids

Specificity

This kit recognizes Rat ALPI in samples. Nosignificant cross-reactivity or interference between Rat ALPI and analogues was observed.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL)

O.D

Average

Corrected

50

2.321 2.363

2.342

2.278

25

1.659 1.681

1.67

1.606

12.5

0.987 0.953

0.97

0.906

6.25

0.415 0.441

0.428

0.364

3.13

0.26 0.236

0.248

0.184

1.57

0.171 0.163

0.167

0.103

0.78

0.113 0.119

0.116

0.052

0

0.06 0.068

0.064

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Kit components & Storage

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.