Place gonads in the cooled chamber of a critical point drier with the samples being covered with ethanol. To prevent the gonads from being damage when the solvent enters or is vented from the chamber, place the gonads between several layers of filter paper cut with a standard hole punch.

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EMSEMDissectionFIG 1: Scanning electron micrograph of the DTC from dissected gonad. Lateral view. Distal-most end of an adult gonad (dissected away from the rest of the body). Long thin trailing processes of the DTC extend (leftward) across the unsheathed distal arm of the mitotic gonad. A bulge on the leading edge of the DTC (far right) may be the nucleus being pushed forward. (Image source: L. Hoffman and D. Greenstein, photo #003.)

EMSEMDissectionFIG 2: Spermatheca viewed by SEM after dissection. Lateral view. Distal-most end of an adult gonad (dissected away from the rest of the body). Long thin trailing processes of the DTC extend (leftward) across the unsheathed distal arm of the mitotic gonad. A bulge on the leading edge of the DTC (far right) may be the nucleus being pushed forward. (Image source: L. Hoffman and D. Greenstein, photo #003.) References