Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia.

Catalog #

A3340-14C

Applications

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution

Peptide ELISA: 1:2000

Western Blot: 0.03-0.1ug/ml. Detected a band at ~35kD in human liver lysates. Additional bands of unknown identity were also observed at 19-22kD. These bands were successfully blocked by incubation with the immunizing peptide.

Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.