Effects of MS65 on genes expression of (a) H1R, PKC, c-Raf, MEK, ERK and (b) IKK-β, IκB-α, NF-κβ. Cells were treated with 10 μM of histamine for 24 h in presence or absence of MS65. Total mRNA was extracted, and gene expression was analyzed by RT-qPCR. The fold expressions were normalized by GAPDH expression levels. Data are expressed as the mean ± S.E.M. of three separate experiments. Values of *P < 0.05, **P < 0.01 and ***P < 0.001 were considered significantly different to histamine-induced control group