We look forward to hearing about your research needs and how we can help. We will strive to understand the details of what you are doing, advise which instruments will most advance your research, train you on the relevant equipment and provide continued assistance. We are here to help you and can consult on all aspects of your project, from sample preparation, to image acquisition and data analysis.

The MSL is a UNC core facility that is part of the Department of Pathology and Laboratory Medicine. We are also the light microscopy core for the Lineberger Comprehensive Cancer Center.

We look forward to hearing about your research needs and how we can help. We will strive to understand the details of what you are doing, advise which instruments will most advance your research, train you on the relevant equipment and provide continued assistance. We are here to help you and can consult on all aspects of your project, from sample preparation, to image acquisition and data analysis.

The MSL is a UNC core facility that is part of the Department of Pathology and Laboratory Medicine. We are also the light microscopy core for the Lineberger Comprehensive Cancer Center.

PRICE FOR NON PROFIT: $78/hour
* Imaging of EM samples by highly-trained staff, with over 30 years of combined experience.
* Consultation for EM projects: advice on sample preparation, analysis, etc.
Note:
* These services are charged in addition to any EM imaging time and sample preparation services.

PRICE FOR NON PROFIT: $78/hour
* Imaging of EM samples by highly-trained staff, with over 30 years of combined experience.
* Consultation for EM projects: advice on sample preparation, analysis, etc.
Note:
* These services are charged in addition to any EM imaging time and sample preparation services.

Technical details:
This system illuminates samples with a light-sheet that is approximately 5um-thick at its narrowest point in the Z dimension. To avoid shadowing artifacts and allow full imaging of thick samples, 3 angled light-sheets can illuminate the sample from each side. In addition, the light-sheet can be scanned horizontally in the X dimension to avoid a loss of Z resolution near the edges. The system comes equipped with five laser lines (488, 514, 561, 647, 785nm) and filter sets compatible with all common fluorophores from the green to far red regions of the spectrum. The system will be outfitted with a 1.3X/0.1 (coming in January) and a 4X/0.3 objective (currently available), as well as a 1X/2X magnification slider, for a total of 4 possible magnifications. The objectives have working distances of 9 and 5.5mm respectively, and the system can easily accommodate samples around 10mm in diameter; which is more than sufficient to image an entire adult mouse brain. These objectives and the sample mounting system are fully compatible with immersion media used in all clearing protocols, including those that require harsh organic solvents. The system has an automated stage that allows easy tiling of large specimens.

PRICE FOR NON PROFIT: $11.2/hour
Access to an incubator custom-built incubator microscope, which is capable of time-lapse imaging at multiple locations in phase contrast mode and three fluorescence channels over several days with stable focus, temperature and CO2 concentration.

PRICE FOR NON PROFIT: $11.2/hour
Access to an incubator custom-built incubator microscope, which is capable of time-lapse imaging at multiple locations in phase contrast mode and three fluorescence channels over several days with stable focus, temperature and CO2 concentration.