Volumest (VOLUMe ESTimation) is a ImageJ plugin for volume estimation using stereological method.
Volumest is published under GPL free software license. Source code is packaged with binaries to jar files.

Install and requirements

Download latest Volumest_*.jar file and copy it to ImageJ plugins folder. Volumest needs java version 1.5 or greater. Volumest is tested with ImageJ 1.35 and 1.38.
Latest version (Volumest_20101017.jar) is briefly tested with ImageJ version 1.43u.

There were confirmed user reports that Volumest does not work with some newer (since versions 1.39) ImageJ version. Fixed version of Volumest plugin is available for download!

Count the number of slices where object or objects appear. Look at the upper left corner of the image window. There are two
numbers separated with slash (like 34/83). First number shows the number of visible slice, second number shows count of slices.

Answer 4 to "slices to be estimated".

Press "Pilot". Volumest suggests grid width for pilot measurement. Default grid width is one tenth of picture width. One may
change this ratio by pointing mouse to "Pilot" button and scrolling mouse wheel. Choose ratio which suggests grid width
roughly same as measured objects largest width.

Choose appropriate object type: irregular or ellipsoide-like.

Press "OK" to start measurement.

Navigate to the first slice with grid when object of interest is visible (using "prev. grid" or "next grid" buttons or mouse wheel).

Using left mouse button, drag around the borders of object of interest. Objects border appears in yellow crosses, objects interior
with red dots or angles.

When object is ellipsoid like: Answer 4 to "Slices to estimated" and 50 to "Points to be counted".

When object is irregular: Answer 10 to "Slices to estimated" and 250 to "Points to be counted".

Repeat measurement. This time CE should be 0.02 to 0.05.

Is object of interest clearly visible under the grid? If using Volumest first time on CT images then probably not.
Press "setup" button. Select "Grid Setup" tab and mark "Grid type" to "right angles". Decrease "Angle arm length" until
object of interest become clearly visible. Setting that to 5% is often good. When necessary change grid color also. Setting it
to some dark color is often good. Sometimes is nessesary to switch grid display off completely. Press "apply" button.