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Sepacore® Flash Systems X10 / X50

Powerful and flexible purification solutions

The Sepacore® flash chromatography systems X10 and X50 address most requirements for the purification of organic compounds. Whether a crude synthesis mixture or a plant extract has to be purified, the system offers an optimal performance and scale-up capability.

Which accessory is required to connect the ELS Detector C-650 to the Sepacore® X50 system?

To connect the ELSD to the Sepacore® X50 system you need the flow splitter kit. The flow splitter kit is designed to provide a small flow rate to the ELSD C-650 (< 5ml/min) and is compatible with an inlet flow rate up to 250 mL/min.

What is the maximum sample load that can be purified using an X50 system?

The biggest column which can be used is a BUCHI glass column 920 mm x 100 mm. Such a column can be packed with about 3700 g silica (40 – 63 µm). This means than the maximum sample load can be higher than 300 g (depending on type of sample).

What are the benefits of a 50 bar (725 psi) pumping system?

It allows running reversed phase separations
even at high flow rates. The solvents used in
reversed phase applications (such as water /
MeOH) have a higher viscosity than the ones
used for normal phase separations: in particular
the mixtures of water and methanol have
a viscosity twice as large as water alone. This
is the reason why only a medium pressure
pump can be used for high scale reversed
phase purifications.
Such a medium pressure is also useful to
work with smaller particle size columns: BUCHI
Silica HP is 15μm instead of 40 - 63μm
for other standard packing materials and the
back pressure generated significantly grows
when the particle size decreases: Back pressure
= k / (particle size)2.