Matrices listed below were spiked with certain level of TGFBI/βIG-H3 and the recovery rates were calculated by comparing the measured value to the expected amount of TGFBI/βIG-H3 in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

85-103

95

EDTA plasma(n=5)

85-105

97

heparin plasma(n=5)

85-99

93

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TGFBI/βIG-H3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.