Degradation of Fumonisin B1 by a Bacterial Strain Isolated from Soil

Abstract A mixed microbial culture degrading fumonisin B l was obtained from soil samples using an enrichment culture procedure. A bacterial isolate from the enrichment culture (strain NCB 1492) degraded fumonisin B1 after incubation for 3 h, as indicated by TLC and HPLC analysis. On the basis of the sequence analysis of 16S rDNA, strain NCB 1492 was related to the Delftia/Comamonas group. Thin-layer chromatographic analysis indicated the presence of metabolites in the NCB 1492 culture filtrates after degradation of fumonisin B1 supplied as sole carbon and nitrogen source in phosphate buffer. Four metabolites were identified by mass spectrometry analysis.

Filtering organic solvents for applications such as HPLC buffer prep can be challenging in order to ensure chemical compatibility with filters (PVDF, Nylon, or PTFE), preserving longevity of an HPLC column, or containment of toxic fumes, but finding a suitable and comprehensive vacuum system is now easier than ever.
Sterlitech is now offering a comprehensive benchtop vacuum system, the Rocker-VF12 Kit, targeted to the purification of organic or corrosive liquids such as mobile phase solvents for HPLC analysis....

Reveleris® Prep: Purification with flash chromatography followed by a prep-HPLC isolation of citral isomers
Lemongrass is cultivated around the world. It`s mainly grown for its oils, which have a number of uses including for vitamin A, perfumes, insect spray, cosmetics, perfumes, food and drinks. Lemongrass is also enjoyed as a tea throughout the world. Lemongrass oil contains several monoterpenes, with citral being the major component, present at levels between 65-85%. Citral (3,7-dimethyl-2,6- octadienal)...

AbstractA high performance liquid chromatography (HPLC) method with a Corona Charged Aerosol detector for the cleaning validation of MICRO* A07 Citric Acid Cleaner residue has been developed. The pooled average repeatability of 3 replicates at 6 concentration levels expressed as RSD was 1.3 % while the pooled average intermediate precision (intra-day repeatability in 3 days) of 3 replicates at 6 concentration levels was 2.4 %. Simulated maximum allowable carryover (MAC) was calculated by using 10 ppm of MICRO®...

Recently, Ade Kujore (Cecil Instruments), Tracie Brombos (GenTech Scientific) and I were asked by Angelo De Palma, a well-respected writer for Lab Manager magazine, to contribute to an article about maintaining and caring about your laboratory instruments.
Here are my tips/best practices for column and HPLC/UHPC/UPLC system maintenance.
Maintaining your LC columns
Proper storage of HPLC columns will increase longevity and performance. For long-term storage, it is best to follow manufacturer’s recommendatio...

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