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In the Kingdom of Youth, Mario, Luigi, Toad and Princess Toadstool are all walking down a path wielding magnifying glasses looking at footprints, with Mario stating that King Goo Goo Ga Ga Koopa is somewhere in the kingdom, planning to cause mischief with the Kingdom of Youth's Fountain of Youth. Eventually, Toad finds the end of the trail of footprints, which are revealed to be nothing more than paper-thin pads; as soon as Toad informs Mario, Luigi and Princess Toadstool that they have reached the end of the footprint trail, an old jalopy, driven by three old men, all of whom are wearing clothes commonly associated with teenagers, drives by rather noisely. Stopping their jalopy, the three old men exit it and after proclaiming they are going to the Fountain of Youth, jump down a nearby slide.

Hearing the old men talk about the Fountain of Youth, Mario, Luigi, Toad and Princess Toadstool decide to follow the three old men down the slide, with Mario hoping that there will be food at the bottom of the slide. After some sliding, the three old men are, somewhat roughly, deposited near the Fountain of Youth, with one of the old men saying that the Fountain of Youth will make him and his companions eighteen again. As the three old men approach the Fountain of Youth, King Goo Goo Ga Ga Koopa, snickering, says to himself that soon the old men will work for him; after stating this, King Goo Goo Ga Ga Koopa turns a nearby dial attached to the Fountain of Youth, which causes a large amount of water to flow into the Fountain of Youth.

Not noticing the excess amount of water pouring into the Fountain of Youth, the three old men jump into the Fountain of Youth and begin to splash each other; after some splashing, the three old men are suddenly changed into babies, who crawl out of the Fountain of Youth and are quickly grabbed by King Goo Goo Ga Ga Koopa. Taunting the babies, King Goo Goo Ga Ga Koopa has his nose grabbed by one, causing him to yelp in pain, dropping the babies as he soothes his nose.

Realizing that he has dropped the babies, who are now running away, King Goo Goo Ga Ga Koopa calls for his Albatoss minions to appear and capture the fleeing babies. Hearing King Goo Goo Ga Ga Koopa's command, several Albatoss, all of whom are brandishing Bomb Nets, appear and begin to drop their Bomb Nets on the babies. Approaching the fleeing babies, the Albatoss drop their Bomb Nets on them, which fall on to and trap the babies, allowing King Goo Goo Ga Ga Koopa to capture the babies with little effort.

King Goo Goo Ga Ga Koopa

Mario, Luigi, Princess Toadstool and Toad, having recently been deposited from a slide, notice, from behind some nearby pillars, King Goo Goo Ga Ga Koopa capturing of the children, who Mario realizes are the three old men who had been encountered by him, Luigi, Toad and Princess Toadstool earlier. As King Goo Goo Ga Ga Koopa enters a nearby fortress, carrying the three babies, Luigi hears a noise emanating from the fortress. As Luigi presses his ear against a wall of the fortress, Luigi is instructed by Mario and Princess Toadstool to look in a window of the fortress with them. Inside the fortress, Mario, Luigi and Princess Toadstool see several babies doing a variety of tasks, such as carrying logs and polishing a floor, all under the supervision of Koopa Troopas.

As Mario, Luigi and Princess Toadstool relent that what King Goo Goo Ga Ga Koopa is forcing these babies to do is terrible, Toad, who is behind Mario, suddenly trips, knocking Mario, Luigi, Princess Toadstool and himself into a wall of King Goo Goo Ga Ga Koopa, which happens to be made of numbered blocks, knocking it down. Hearing the wall collapse, King Goo Goo Ga Ga Koopa notices Mario, Luigi, Toad and Princess Toasdstool lying on the floor and leaps on to a nearby, wheeled rocking horse, which resembles an Ostro and begins to wheel after them. As Luigi, Toad and Princess Toadstool run from King Goo Goo Ga Ga Koopa, Mario, grabbing a block, which fell from the collapsed wall, throws it at King Goo Goo Ga Ga Koopa, causing him to crash his rocking horse.

Catching up to Luigi, Toad and Princess Toadstool, Mario begins to run from King Goo Goo Ga Ga Koopa's fortress with them; unfortunately, as she runs, Princess Toadstool trips and falls into the nearby Fountain of Youth and begins to flail about in the Fountain of Youth's deep water. Before Princess Toadstool can drown though, Mario, Luigi and Toad manage to pull her out of the Fountain of Youth, only to watch, dumbstruck, as Princess Toadstool transforms into an infant right in front of them.

While staring at the now juvenile Princess Toadstool, Mario, Luigi and Toad are approached by King Goo Goo Ga Ga Koopa, who is still riding his rocking horse. While King Goo Goo Ga Ga Koopa taunts Mario, Luigi and Toad, Princess Toadstool, reaching into Mario's overalls, pulls a plunger from them and quickly throws it at King Goo Goo Ga Ga Koopa's face, which the plunger becomes stuck to.

While King Goo Goo Ga Ga Koopa begins trying to pull the plunger off his face, Mario, grabbing Princess Toadstool, begins to run away with Luigi and Toad. As Mario, Luigi, Toad and Princess Toadstool run, King Goo Goo Ga Ga Koopa, managing to pull the plunger off his face, orders his Koopa Pack, which consists of Albatoss wielding Bob-ombs, to attack Mario, Luigi, Toad and Princess Toadstool. As King Goo Goo Ga Ga Koopa chases Mario, Luigi, Toad and Princess Toadstool on his rocking horse, he orders his Albatoss minions to use their Bomb Nets on Mario, Luigi, Toad and Princess Toadstool; following King Goo Goo Ga Ga Koopa's order, an Albatoss drops a Bomb Net, which explodes and releases a net, which misses Mario, Luigi, Toad and Princess Toadstool by several feet; though the net does succeed in causing Mario to trip, which causes him to accidentally fling Princess Toadstool through the air. As Princess Toadstool flies happily through the air, she is caught by Luigi before hitting the ground; with Princess Toadstool safely in his arms, Luigi reprimands Mario, saying he should be more careful with Princess Toadstool. As Mario, Luigi, Toad and Princess Toadstool continue to run from him, King Goo Goo Ga Ga Koopa becomes entangled in a net dropped by an Albatoss and is left furiously trying to escape.

As King Goo Goo Ga Ga Koopa is left trying to untangle himself from the net, Mario, Luigi, Toad and Princess Toadstool reach a nearby playground. After arriving in the playground, Mario quickly instructs Toad, Luigi and Princess Toadstool to go on to two nearby seesaws; before Mario can enact a plan, though, he is attacked by a Koopa Troopa riding a rocking horse and wielding a large safety pin. Managing to evade the attacking Koopa Troopa, Mario accidentally slips on a nearby roller skate and falls on to the opposite ends of the seesaws Luigi, Toad and Princess Toadstool are standing on, which causes Luigi, Toad and Princess Toadstool to be flung through the air. After soaring through the air for a small amount of time, Luigi, Toad and Princess Toadstool safely land on the other side of a large wall.

In the playground, Mario is in the midst of running from several Albatoss, who are dropping Bomb Nets and Bob-ombs on him. While running from these Albatoss, Mario notices a rope hanging from a tree and uses it to swing through the air and over a wall, on the other side of which, he meets Luigi, Toad and Princess Toadstool. Greeting Luigi, Toad and Princess Toadstool, Mario tells them to run, saying that they will save the babies King Goo Goo Ga Ga Koopa has captured later on, at night.

Later, Mario, Luigi and Toad, who are in a large field, are pushing Princess Toadstool on a swing, with Mario complaining that he knows about baby brussel sprouts, mini frozen pizzas and tiny tortellinis, but nothing about babies. Hearing Mario's complaints, Toad reassures him, saying that taking care of Princess Toadstool should be easy, as all they need to do is feed her when she's hungry and wash her when she's dirty. After Toad finishes talking though, Luigi is shocked to find that Princess Toadstool is no longer on the swing. Panicking, Mario, Luigi and Toad, after looking around, see Princess Toadstool crawling along the branch of a nearby tree, trying to catch a green bird that is sitting on the end of the tree branch. Fearing that Princess Toadstool will fall from the tree branch she is on, Mario begins to climb up the tree after her. Getting on the branch Princess Toadstool is crawling on, Mario tries to persuade Princess Toadstool to crawl over to him; unfortunately, as Mario tries to tempt Princess Toadstool to come over to him, saying he has a big surprise for her, the branch Mario and Princess Toadstool are on breaks.

Sent tumbling towards the ground, Mario lands in a pile of leaves, while Princess Toadstool bounces off a branch of the tree. Rushing to Mario, Luigi and Toad point-out to him that Princess Toadstool, who is now holding a twig, has bounced directly into a nearby canyon with a river at the bottom of it. As she falls into the canyon, giggling, Princess Toadstool's diaper gets caught on a tree branch sticking out of a wall of the canyon. Seeing that there is still a chance to save Princess Toadstool, Mario, using some tree branches and a large leaf, creates a hang glider and uses it to swoop down into the canyon where Princess Toadstool is. Getting close to Princess Toadstool, Mario grabs her and begins to glide back up the canyon; as Mario glides though, Princess Toadstool begins to tickle him, which causes Mario to laugh so hard that he loses his grip on his hang glider and is sent plummeting down into the canyon below, while Princess Toadstool safely descends into the canyon, still hanging on to the hang glider.

Luckily, Mario survives his fall into the canyon by tumbling into a large pile of mud; shortly after Mario lands in this pile of mud, Princess Toadstool lands next to him and proclaims that he is a lot of fun to be with. Later, as Mario washes some mud off himself by a waterfall, Toad tells Mario once again that babysitting isn't too hard and keeps watch on Princess Toadstool, who is sitting on a nearby raft. Wanting to keep Princess Toadstool occupied and out of the river her raft is on, Toad gives her a pinwheel; once Princess Toadstool gets this pinwheel though, she blows on it with enough force to move the raft she is on down the river. As Princess Toadstool is propelled down the river, with her raft being slowly eaten by Trouters, Toad, panicking, yells for Mario and Luigi to help him save Princess Toadstool from being eaten by voracious Trouters.

As she flows down the river on a raft, Princess Toadstool, oblivious to the danger surrounding her, grabs a Trouter from the river and throws it towards Mario, Luigi and Toad; attempting to flee this Trouter, Mario, Luigi climb a tree to escape it while Toad runs from it. This tree offers little aid to Mario or Luigi though, as the Trouter simply chews through the trunk of it, causing the tree to fall into the river. As Mario and Luigi sail down the river on the collapsed tree, Toad, using several rocks as stepping stones, manages to hop on to the tree Mario and Luigi are on. As they sail down the river on the tree, Mario and Luigi, after being alerted by Toad to the nearby Princess Toadstool, whose raft is almost been entirely eaten by Trouters, take out their plungers and use them as oars to pull alongside Princess Toadstool's raft. Once close enough to Princess Toadstool, Mario, after tying a rope to his plunger, uses it to grab Princess Toadstool and pull her in to the arms of Luigi. Toad then complains that there isn't much sitting in babysitting, but Luigi assures him that there will be once they get her to take a nap.

Later, somewhere in the Kingdom of Youth, Mario, Luigi and Toad are in the middle of rocking Princess Toadstool back and forth on a hammock, with Mario singing lullabies to Princess Toadstool. When Mario finishes his lullaby, he, Luigi and Toad, briefly watching the sleepy Princess Toadstool (while reminding each other to not to disturb her), begin to walk away from her, only to be forced to rock Princess Toadstool back and forth again when she begins crying. Sometime later, Toad finally manages to calm Princess Toadstool down by amusing her with two fireflies he has captured; after leaving Princess Toadstool with the fireflies, Toad walks over to the nearby Mario and Luigi and tells them that Princess Toadstool isn't that hard to take care of and motions towards her, only for him, Mario and Luigi to see that Princess Toadstool is gone, having followed the two fireflies all the way to King Goo Goo Ga Ga Koopa's castle.

Baby Toadstool crawling along the ground near the Fountain of Youth

In the front of King Goo Goo Ga Ga Koopa's castle, Princess Toadstool is crawling along the ground near the Fountain of Youth, chasing several fire flies. Having caught-up to Princess Toadstool, Toad sees that she is going to fall into the Fountain of Youth and quickly has Mario rush over to her and pick her up before Princess Toadstool falls into the Fountain of Youth again. It is only when he gives Princess Toadstool to Toad that Mario sees that King Goo Goo Ga Ga Koopa has taken the controls of the Fountain of Youth. As Luigi bemoans the loss of the Fountain of Youth's controls, saying without them, Princess Toadstool will never be returned to normal, Mario cheers him up, saying that all that needs to be done to repair the Fountain of Youth is to reverse the flow of its water. After saying this, Mario goes over to King Goo Goo Ga Ga Koopa's castle and opens a door on it, exposing a room filled with pipes.

After getting all the pipes they need, Mario and Luigi, after some work, make a large piping system extending from the fountain. Before either Mario or Luigi can activate this piping system though, King Goo Goo Ga Ga Koopa, riding an Ostro rocking horse, barges out of his castle towards Mario and Luigi. As King Goo Goo Ga Ga Koopa barrels down towards them, Mario and Luigi, grabbing a large pipe, use it to catapult King Goo Goo Ga Ga Koopa through the air and into the Fountain of Youth. After crashing into the Fountain of Youth, King Goo Goo Ga Ga Koopa emerges from the Fountain of Youth as an infant version of himself and storms off, sobbing and vowing revenge on Mario and Luigi.

With King Goo Goo Ga Ga Koopa defeated, Mario turns on his piping system, which causes the dial on the Fountain of Youth to reset itself, it also causes the water in the Fountain of Youth to flow backwards. As the Fountain of Youth flows in reverse, all the children King Goo Goo Ga Ga Koopa had captured surge forth out of his castle and leap into the Fountain of Youth, while Toad lowers Princess Toadstool into the Fountain of Youth. Upon touching the water of the Fountain of Youth, Princess Toadstool reverts to her normal form, as do all the other surrounding babies, who, upon turning back to normal, exclaim that they are happy to be back to normal and to be able to stay up until 10pm again.

With King Goo Goo Ga Ga Koopa vanquished from the Kingdom of Youth and everything in it returned to normal, Mario, Luigi, Toad and Princess Toadstool decide to leave the Kingdom of Youth on several rocking horse Ostros; as she rides behind him, Princess Toadstool says to Mario that if he ever decided to quit his job as a plumber, he could become a babysitter, to which Mario responds that the only sitting he wants to do is to a big plate of pasta and finally the four laugh.

When Princess Toadstool transforms into an infant, her normal form fades out and her infant form fades in, instead of her normal form morphing into her infant form.

When Mario and Luigi raise a pipe against the approaching King Goo Goo Ga Ga Koopa, Luigi is shown as having Mario's hat on his head. Additionally, in the same shot, Mario is speaking with Luigi's voice instead of his own voice when speaking to Luigi and even refers to him as Mario.

As Baby Toadstool is being flung in the air, the bottom part of the screen shows a black area for a half second.

When Mario yells "Luigi!" after being tripped by the net, his mouth doesn't move.

This is the first episode of any DIC Mario cartoon where Princess Toadstool undergoes a magical transformation. The second is "The Trojan Koopa" in which her clothing changes to the colors of the American flag.

When this episode first aired, the cartoon introduction was missing. It was later added to subsequent airings.