Map of human serum fibrinogen (Fg) on a polystyrene
(PS), polymethylmethacrylate (PMMA) blend derived from a C 1s X-PEEM
image sequence. The fibrinogen (blue) has a strong preference for
adsorption on the PS domains (red). The yellow in the PMMA domains
(green) arises from PS microdomains (R+G=Y - see color circles) and
some radiation damage.
Details: The fibrinogen was adsorbed from a 0.05 mg/ml pH=7
phosphate buffer solution onto a phase segregated polymer blend
formed by spin coating a 30% polystyrene (PS), 70%
polymethylmethacrylate (PMMA) blend from dilute toluene solution (1
wt% polymer). A C 1s image sequence (49 energies, 280-320 eV) was
recorded using the photoemission electron microscope (PEEM-2) at the
ALS and analyzed to generate component maps which were then combined
into this color composite map.