Flow Cytometry (intracellular, 0.5-5µg per 106 cells; cell surface: see Xu et al. (2006) for details)Immunohistochemistry (paraffin sections: 5µg/ml)Western Blot (1-3µg/ml)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Application Notes:

Detects a band of ~120kDa by Western blot.Flow Cytometry: Suitable for cell surface and intracellular FC.

Purity Detail:

Protein G-affinity purified.

Formulation:

Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.

Handling:

Avoid freeze/thaw cycles.

Shipping:

Shipped on Blue Ice

Short Term Storage:

+4°C

Long Term Storage:

-20°C

Scientific Background:

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TLR8 gene contains three exons, two of which have coding function. TLR8 cDNA codes for a protein of approximate molecular weight of 120kDa.

Technical Info/Product Notes:

Replacement for ADI-CSA-825

Figure: Western blot analysis of TLR8 in cell lysates from 293 transfected with human TLR8 using ALX-804-376 at a dilution of 2µg/ml. "