Re: [Histonet] Alizarin red/Alcian blue double skeletal stain

From:

John Kiernan

There's this method:
Webb GN & Byrd RA 1994. simultaneous differential
staining of cartilage and bone in rodent fetuses: an
alcian blue and alizarin red S procedure without
glacial acetic acid. Biotechnic & Histochemistry
69(4):181-185.
The colour photo looks excellent. The instructions take
up 2 printed pages so more is needed than a simple
"protocol." The journal is available in all good
medical libraries. The method could probably be
simplified for chick embryos because they are smaller
than rat fetuses.
--
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
kiernan@uwo.ca
http://publish.uwo.ca/~jkiernan/
_______________________________________
"Grant, Debra" wrote:
>
> Does anyone have a protocol for Alizarin Red/Alcian Blue on chick embryo? I have several protocols for mouse. I have tried to adjust times in solution, but I have some problems with the cartilage staining in some of the chick embryos.
> Thank you in advance.
>
> Debby Grant
> Research Technician II
> Histology Core Facility
> Stowers Institute for Medical Research
> 1000 E. 50th Street
> Kansas City, MO 64110
> drg@stowers-institute.org
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