I would be much grateful if someone share some info on how a simple
resin such as Chelex 100 is able to aid in the lysis of Gram+ bacteria
and endospores. For example many authors use chelex-based methods to
extract DNA from Bacillus spores. In the article by A Stein and D
Raoult. A simple method for amplification of DNA from paraffin-embedded
tissues. Nucleic Acids Res. 1992 October 11; 20(19): 5237–5238. is
noted that a simple boiling did not produce PCR amplifiable DNA where
as boiling in Chelex did. I know that the resin binds a large portion
of the PCR inhibitors but how is it supposed to participate in the
lysis step ?