The purpose of this assay is to assess the specificity of compounds identified as inhibitors of KCNK9. This specificity screen is to eliminate any compounds that interact with the inwardly-rectifying channel, Kir2.1. The protocol as in AID 1672 is used for this assay except each compound is tested at 10 concentrations. Compound inhibition of KIR2.1 was tested in at least duplicate using a thallium sensitive dye.

Possible artifacts of this assay can include, but are not limited to: non-intended chemicals, or dust in or on wells of the microtiter plate, compounds that non-specifically modulate the cell host or the targeted activity, and compounds that quench or emit light or fluorescence within the well. All test compound concentrations reported are nominal; the specific concentration for a particular test compound may vary based upon the actual sample provided by the MLSMR. The conditions of this assay are optimal for screening for compounds that modulate KCNK9 channels, NOT for the assay of Kir2.1 modulators. Normalization is to this set of data and cannot be used for comparison with other counter screens.