Isolation and characterization of two cyclin-like cDNAs from Arabidopsis

Isolation and characterization of two cyclin-like cDNAs from Arabidopsis
Day, Irene; Reddy, A.S.N.
2004-10-06 00:00:00
Cyclins are key regulators of a family of protein kinases called cyclin-dependent kinases (Cdks). Specific cyclins interact with specific Cdks to regulate the different transition points in the cell cycle. Six mitotic-like cyclins have previously been reported in Arabidopsis thaliana. Using polymerase chain reaction amplified cyclin-box sequences as probes, two new cyclin cDNAs are isolated from Arabidopsis. The deduced amino acid sequences of the isolated cDNAs (Arath;CycB1;3 and Arath;CycB1;4) show the highest sequence similarity with mitotic cyclins. Arath;CycB1;3 is most homologous to the plant CycB1group cyclins and contains a conserved motif that is typical of this group. Arath;CycB1;4, while homologous to Arath;CycB1;2, has some features that make it different from other known mitotic-like cyclins. These data suggest the presence of several distinct cyclins of CycB1 group in Arabidopsis. Analysis of expression of three members of CycB1 group (Arath;CycB1;2, Arath;CycB1;3 and Arath;CycB1;4) in different tissues by reverse transcription-polymerase chain reaction using primers corresponding to unique regions of their cDNAs shows that they are differentially expressed in different tissues.
http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.pngPlant Molecular BiologySpringer Journalshttp://www.deepdyve.com/lp/springer-journals/isolation-and-characterization-of-two-cyclin-like-cdnas-from-RvJ9XfJEMq

Isolation and characterization of two cyclin-like cDNAs from Arabidopsis

Abstract

Cyclins are key regulators of a family of protein kinases called cyclin-dependent kinases (Cdks). Specific cyclins interact with specific Cdks to regulate the different transition points in the cell cycle. Six mitotic-like cyclins have previously been reported in Arabidopsis thaliana. Using polymerase chain reaction amplified cyclin-box sequences as probes, two new cyclin cDNAs are isolated from Arabidopsis. The deduced amino acid sequences of the isolated cDNAs (Arath;CycB1;3 and Arath;CycB1;4) show the highest sequence similarity with mitotic cyclins. Arath;CycB1;3 is most homologous to the plant CycB1group cyclins and contains a conserved motif that is typical of this group. Arath;CycB1;4, while homologous to Arath;CycB1;2, has some features that make it different from other known mitotic-like cyclins. These data suggest the presence of several distinct cyclins of CycB1 group in Arabidopsis. Analysis of expression of three members of CycB1 group (Arath;CycB1;2, Arath;CycB1;3 and Arath;CycB1;4) in different tissues by reverse transcription-polymerase chain reaction using primers corresponding to unique regions of their cDNAs shows that they are differentially expressed in different tissues.