PEGylation Reagents (PEG Labeling)

We offer activated linear and branched derivatives of polyethylene glycol (PEG) for pegylation and the PEG-modification of peptides and proteins via primary amines and sulfhydryl groups. These reagents are typically used to increase solubility, prolong stability, and reduce immunogenicity. Certain experimental systems and assay platforms depend on the ability to alter the mass, solubility or other properties of proteins, immunogens, reaction vessels and other materials.

Amine-reactive PEGylation reagents

Covalent modification with PEG groups requires PEG compounds that contain a reactive or targetable functional group at one end. The simplest method for PEGylating proteins, which are rich in surface primary amines, is to use a PEG compound that contains an NHS ester group at one end.

MS(PEG)n reagents are available in PEG lengths (n = 4, 8, 12 and 24).

TMS(PEG)n is a branched form of this reagent, containing three methyl-PEG12 arms attached to a PEG4-NHS ester stem.

Sulfhydryl-reactive PEGylation reagents

MM(PEG)n compounds are linear reagents for PEGylating sulfhydryl groups. At the end opposite the inert methyl group(s) is a maleimide moiety, which reacts to form stable thioether bonds with sulfhydryl groups.

MM(PEG)n is available in two PEG lengths (n = 12 and 24).

PEGylated amino acids and amine compounds

MA(PEG)n and CA(PEG)n are polyethylene glycol compounds of discrete length (n = 4, 8, 12 and 24) containing methyl-and-amine or carboxyl-and-amine ends. While these functional groups are not spontaneously reactive, they are easily targeted by various crosslinking and immobilization reagents for the construction of surface chemistries.

Choose the right PEGylation reagent for your experiment

PEGylation reagents

Product

Spacer arm (Å)

Amine-Reactive PEG (Linear)

Reactive group: N-hydroxysuccinimide ester

Target functional group: Primary amines

Application: PEGylation of a protein or surface, terminating with a methyl group

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