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705-Cre;cmCre Expression Plasmid with chloramphenicol resistance marker for use in E.coli onl

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Description

705-Cre plasmid is designed for use in Cre-mediated genomic manipulations. The plasmid has a pSC101 origin which maintains low copy and replicates at
30°C. The plasmids will not propagate and will get lost when incubated at 37°C.
The expression of the Cre-recombinase is driven by the thermosensitive promoter cI578 (λPR promoter). Therefore, the expression of Cre is repressed at 30°C and induced between 37-42°C. The plasmid carries a chloramphenicol resistance.

Note: The sequence of 705-Cre was compiled from information found in the sequence databases, published literature, and other sources, together with partial sequences obtained by Genebridges. This vector has not been completely sequenced. The digestion patterns for BamHI, EcoRI, HindIII, PstI, XbaI and XhoI are indicated.