C. Difficile Spores Spread Superbug

Date:

July 21, 2009

Source:

Wellcome Trust Sanger Institute

Summary:

A team of scientists have modeled the infection cycle of C. difficile by generating a "mouse hospital" with conditions mimicking transmission in the human environment. The scientists suggest that antibiotic treatment could be asymptomatically inducing the transmission of healthcare-acquired C. difficile, contributing to the outbreaks that have been widely reported in hospitals and other settings. The results have implications for infection control measures and open the door for improved diagnosis of C. difficile.

Share:

Total shares:

FULL STORY

New research suggests that antibiotic treatment could be asymptomatically inducing the transmission of the healthcare-acquired infection, C. difficile, contributing to the outbreaks that have recently been widely reported in hospitals and other settings. A team of scientists have successfully mirrored the infection cycle of C. difficile by generating a 'mouse hospital' with conditions mimicking the human environment in which C. difficile is transmitted.

The results have implications for infection control measures in the healthcare environment and open the door for the development of treatments and improved diagnosis of C. difficile.

At present, healthcare professionals manage the threat of C. difficile by observing stringent hygiene and isolation practices primarily by dealing with patients who exhibit the symptoms of infection - including diarrhoea and fever. But today's publication suggests that widening the targets of infection control in hospitals, to include all patients receiving antibiotic treatment - although logistically complex - is worth investigating.

"C. difficile is a highly resistant and highly infectious pathogen and resistant to many front line antibiotics," explains Dr Trevor Lawley, Wellcome Trust Sanger Institute researcher and lead author on the study. "Until now, animal studies have focussed on the observable, acute symptoms of C. difficile. But, to understand how this highly infectious pathogen spreads, investigating the entire cycle of transmission is absolutely vital. We looked at mice carrying C. difficile and observed that they shed low levels of spores and, crucially, they did not infect other mice."

"But when we treated mice with antibiotics, we saw a dramatic rise in the levels of spores shed - leading to what we have described as a 'supershedder state' and transmission of C. difficile among mice. Importantly, transmission occurs even in the absence of clinical symptoms."

C. difficile transmission relies on the shedding of highly resistant spores in the faeces of humans. These bacterial spores are essentially dormant cells with protective outer layers making them well-adapted for survival and dispersal in a wide range of environmental conditions. When humans shed spores in their faeces, those spores are capable of surviving dormant in the environment for long periods of time, under harsh conditions and in temperatures up to 70 ºC, before reintroduction and infection in a new human host.

"We treated mice with short and longer courses of antibiotics," says Professor Gordon Dougan, Head of Pathogen Genetics at the Sanger Institute and senior author on the study. "After a short course most mice had dropped back to normal spore shedding levels around two weeks after cessation of the treatment. But after long term exposure to antibiotics some of the mice remained in their 'supershedder' state for weeks or even longer after treatment was stopped. We should consider that patients still pose a considerable transmission threat some weeks after treatment is terminated even if they have not exhibited signs of C. difficile disease."

The team also found that there was a considerable threat from environmental contamination. Even short-term housing of 'supershedder' mice in transfer vessels could contaminate the area, leading to infection in naïve - or uninfected - mice, and suggesting that even the briefest environmental contamination is a potential infection threat.

The administration of antibiotics has been a known risk factor in the development of C. difficile infection for some time. The bacterium can live inside the gut of healthy human beings, existing as part of a natural, diverse and potentially beneficial ecosystem of bacteria and microorganisms –the microbiota - that we are all are host to.

When the team treated mice with antibiotics, the balance of the microbial ecosystem was disrupted. Because C. difficile is resistant to many antibiotics, the bacterium was able to exploit the opportunity and proliferate where other gut bacteria had succumbed to the antibiotics. This allowed the C. difficile to flourish and dominate the microbiota of the mouse.

Although the majority of mice exhibited no visible symptoms of C. difficile, the team found that one particular group of knockout mice with mutations associated with impaired immune defence began to lose weight and became moribund. The similarity with the human manifestation of the disease, where older or immune impaired patients tend to exhibit the symptoms of acute C. difficile infection, is clear and strengthens utility the mouse model as a valuable tool for investigation into the transmission of C. difficile.

Today's results are published soon after a previous publication, where the team demonstrated that C. difficile spores could be isolated and highly purified - essentially washed - in a way that maintains their natural characteristics and resistance to an array environmental conditions. This ability to purify spores, while preserving their characteristics, allows researchers to use them in experimental settings and to establish their structure.

"C. difficile is a high-profile and rapidly emerging pathogen and is responsible for the death of a patient every hour in our hospitals - but its biology and transmission are so far poorly understood," says Brendan Wren, Professor of Microbial Pathogenesis from the London School of Hygiene & Tropical Medicine's Department of Infectious & Tropical Diseases. "At last we can monitor the transmission of this major pathogen using a tractable model system. This will be invaluable in determining the role of spore formation in transmission and how and why some C. difficile strains are more virulent and transmissible than others."

"It will also facilitate determining the host factors important in disease and will lead to better diagnosis and treatment strategies to reduce the burden of C. difficile-associated diseases."

Because the team had established how to isolate and purify spores and trigger transmission of C. difficile in mice, they were also in a position test disinfectants commonly used in hospitals for their success at controlling infection. The alcohol-based disinfectants tested had no success in preventing transmission. Instead, a 20-minute surface disinfection using a sporicidal agent was necessary to reduce environmental spore contamination enough to eliminate transmission. Further results detailing the success of individual hospital disinfectants will be published in the coming months.

Today's publication provides a step towards reducing the burden on healthcare systems, which have seen a ten-fold increase in the incidence of C. difficile in the last decade. 56,000 cases were reported in the UK in 2007, although, the first a three months of 2009 saw a decline in incidence compared to the same period in 2008. The most severe complication is pseudomembranous colitis, an infection of the colon that can be lethal in patients with weakened immune systems.

"What this research essentially provides," says Dr Fiona Cooke, working in the Department of Medical Microbiology at Addenbrooke's Hospital, Cambridge, "is a better understanding of the interactions between C. difficile, the intestinal microbiota and the immune system of the host - in this case the mouse - primarily in response to antibiotic treatment but also in response to immune impaired hosts. This opens up numerous opportunities including the development of new probiotic approaches, which could restore the balance of the intestinal microbiota and promote health, are clear."

"This research has far reaching implications for treatment and infection control of a pathogen that is increasingly raising alarm among the global healthcare community."

July 31, 2015  School is just around the corner, which means backpacks and packed lunches await your children. One expert offers tips for parents to promote healthy dental habits while away from ... read more

July 29, 2015  By blocking the expression of a certain gene in patients, researchers have contributed to the demonstration of great decreases in the concentration of triglycerides in their ... read more

July 29, 2015  Viewing aquarium displays led to noticeable reductions in blood pressure and heart rate, a research team found in the first study of its kind. They also noted that higher numbers of fish helped to ... read more

Jan. 9, 2013  A new study found that many patients with Clostridium difficile infection are prescribed unnecessary antibiotics, increasing their risk of recurrence of the deadly infection. The retrospective report ... read more

Dec. 9, 2012  Researchers show that the global epidemic of Clostridium difficile 027/NAP1/BI in the early to mid-2000s was caused by the spread of two different but highly related strains of the bacterium rather ... read more

Feb. 7, 2012  Contrary to current convention by which infection with the organism Clostridium difficile is regarded as an infection that is acquired by contact with symptomatic patients known to be infected with ... read more

Aug. 11, 2011  An outbreak strain of Clostridium difficile, a bacterium that causes diarrhea and sometimes life-threatening inflammation of the colon, is common in Chicago-area acute care hospitals, research ... read more