Oligo Modification—Label-ON Reagents

Label-ON Reagents provide an efficient way to directly label oligonucleotides with various reporter molecules (1–4). All Label-ON Reagents possess the patented 2-aminobutyl-1,3-propanediol (ABPD) backbone, which offers the following distinct labeling advantages:

Multiple labels can be incorporated because Label-ON Phosphoramidites do not truncate chain elongation at the 5' terminus.

Natural 3-carbon internucleotide phosphodiester distance is maintained, providing better hybridization and annealing properties than those obtained with other labeling methods.

Attached label is separated from the phosphodiester connection point to reduce steric hindrance. The ABPD backbone has a 7-atom spacer arm that separates the label from the connection point.

Features

Incorporate single or multiple labels during oligonucleotide synthesis

Place labels at the 5' end, internally, or at the 3' end

Maintain natural 3-carbon internucleotide phosphate distance

Obtain very high coupling efficiencies on any commercially available synthesizer

Applications

Oligo labeling (reagents)

Nucleic acid modifications

Nucleic acid chemistry (reagents)

Nucleic acid labeling (reagents)

Nucleic acid synthesis (reagents)

References

Nelson, P., et al. (1992) Nucleic Acids Res. 20(23):6253–6259.

Nelson, P. & Muthini, S. (1993) OligoTechniques1(1):1–4.

Nelson, P., et al. (1993) OligoTechniques1(1):5–7.

Nelson, P. & Muthini, S. (1993) Biomedical Products18(6):40.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.