LIS Resources

This assay is performed using the HemosIL Plasminogen Kit on the Beckman Coulter ACL TOP. The method is an automated chromogenic assay in which an excess of streptokinase (SK) in the presence of fibrinogen is added to sample plasma containing plasminogen. A plasminogen-streptokinase complex is formed. The complex catalyzes the splitting of p-nitroaniline (pNA) from the substrate S-2403 pyroGlu-Phe-Lys-pNAHCl. Under these conditions the enzymatic activity of the complex is not inhibited by plasma inhibitors. The rate at which the pNA is released is measured kinetically at 405 nm and is directly proportional to the plasminogen level in the test specimen. The concentration of plasminogen is calculated from a standard curve prepared from reference plasma dilutions.(Friberger P, Knos M: Plasminogen determination in human plasma. In Chromogenic Peptide Substrates: Chemistry and Clinical Usage. Edited by Edinburgh. Churchill Livingstone, 1979, pp 128-140)