Current Medical MycologyCurrent Medical MycologyCurr Med MycolMedical Scienceshttp://cmm.mazums.ac.ir1admin2423-34392423-342010.29252/cmmenjalali139791gregorian201812144online1fulltextenGenotyping of Candida albicans isolated from animals using 25S ribosomal DNA and ALT repeats polymorphism in repetitive sequenceاپیدمئولوژیEpidemiologyپژوهشيOriginal Articles<strong>Background and Purpose:</strong> Candida albicans is the most prevalent Candida species isolated from animals. Candidiasis can be systemic in animals or may affect a single organ, such as the mouth, urinary tract, and skin. The aim of the present study was to determine the genetic diversity of C. albicans isolated from different animals and investigate the presence of a relationship between host specificity and genetic typing of C. albicans.<br>
<strong>Materials and Methods:</strong> For the purpose of the study, DNA extraction was performed on 27 clinical isolates of C. albicans obtained from animals. Subsequently, they were subjected to 25S ribosomal DNA amplification and ALT repeats in repetitive sequences (RPSs). The minimum inhibitory concentrations of fluconazole, ketoconazole, clotrimazole, nystatin, amphotericin B, and caspofungin were determined using the microdilution method based on the Clinical and Laboratory Standards Institute M27-S4 standard.<br>
<strong>Results:</strong> Out of 27 C. albicans strains, 11, 6, 5, and 5 cases were recognized as genotypes A (40.8%), E (22.2%), B (18.5%), and C (18.5%), respectively, through amplification using AS-I, which revealed 17 different types of C. albicans. By combining the two typing methods, 27 C. albicans strains were finally divided into 22 genotypes.<br>
<strong>Conclusion:</strong> Different genotypes showed genetic diversity among the C. albicans strains isolated from animal sources. The results revealed no special genotype relationship according to the host, anatomical source of isolation, and antifungal susceptibility.<br>
&nbsp;Animal, Antifungals, Candida albicans, Genotyping, Repetitive sequences1219http://cmm.mazums.ac.ir/browse.php?a_code=A-10-75-4&slc_lang=en&sid=1FarzadKatiraeekatiraee_f@yahoo.com1003194753284600534310031947532846005343Yes
University of TabrizArminaDalvanda.dalvand69@gmail.com1003194753284600534410031947532846005344NoUniversity of TabrizRaziallahJafari Joozanirazi_allah@yahoo.com1003194753284600534510031947532846005345NoUniversity of TabrizhojjatollahhojjatollahShokrihshokri@ut.ac.ir1003194753284600534610031947532846005346NoAmol University of special modern technologies