Figure 7.

Mutation of the galU gene increases cytotoxicity of FT. Murine macrophage-like cells (J774) were infected with the WT, galU mutant, the galU-complemented, or wbtA mutant (O-antigen-deficient) FT LVS strains at an MOI of 100. Host cell death was
determined by measuring LDH released from infected cells 24-hours post-infection.
All data points represent the mean (± SEM) of triplicate samples and the data shown
is representative of three experiments of similar design. Statistical analyses were
performed via one-way ANOVA with a Bonferroni multiple comparisons post-test (***
indicates a p-value of <0.0001).