check to make sure there are enough LB plates for each group to have 4 total

check to see that there is enough top agar for each group to have 12 mL total

Wednesday before Day 1

Take off 8 125-mL flasks with 40 mL of now-saturated phage culture. Store at 4ºC (this will be for Friday's class)

Use small (yellow-capped) tubes to make titering cells - about 1 mL is necessary for each group, so make up 6 5-mL cultures with 5 mL LB, 5 µL Kan, and a colony of ER2267. Incubate rotating overnight at 37ºC

Each group should make a 0.1 M solution of hexadecyltrimethylammonium bromide ("CTAB"). This is done by mixing 0.72 g with 20 ml of water in a 50 ml conical tube - CTAB will be placed in 30ºC incubator the day before to fully solubilize

One group will be responsible for making each of the following solutions that the class will use in the synthesis of nanowires.