QIAquick Gel Extraction Kit

For gel extraction/cleanup of up to 10 μg DNA (70 bp to 10 kb) from enzymatic reactions

Up to 95% recovery of ready-to-use DNA

Fast and convenient procedure

Cleanup of DNA up to 10 kb in three easy steps

Gel loading dye for convenient sample analysis

The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. DNA ranging from 70 bp to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl. An integrated pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on theQIAcube.
For optimal results it is recommended to use this product together with QIAvac 24 Plus.

The QIAquick Gel Extraction Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

QIAquick and MinElute procedure.

The QIAquick and MinElute systems use a simple bind-wash-elute procedure with spin columns or a vacuum manifold.

High recoveries from gels.

DNA fragments (sizes indicated) before extraction with the QIAquick Gel Extraction Kit and pooled after extraction are shown. Recoveries of approximately 80% are obtained for all fragment sizes [A]1–5: before extraction; P: pooled after extraction. Samples were analyzed on a 1.5% agarose gel in TAE buffer. [B]1–3: before extraction; P: pooled after extraction. Samples were analyzed on a 3.5% high-resolution agarose gel in TAE buffer. M: pTZ-HinfI markers.

The QIAquick system uses a simple bind-wash-elute procedure (see flowchart "QIAquick and MinElute procedure"). Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the QIAquick spin column (see figure "pH Indicator Dye"). Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in all subsequent applications.

Handling

QIAquick spin columns are designed to provide two convenient handling options. The spin columns fit into a conventional table-top microcentrifuge or onto any vacuum manifold with luer connectors, such as the QIAvac 24 Plus with QIAvac Luer Adapters. The QIAquick Gel Extraction Kit, in addition to other QIAGEN spin-column-based kits, can be fully automated on the QIAcube, enabling increased productivity and standardization of results (see figures "Spin column handling options A, B, C, D, and E").

Applications

DNA fragments purified with the QIAquick system are ready for direct use in all applications, including sequencing, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription.