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Western blot analysis of Caspase-3 in HeLa cells. Cells were treated with 2 mM staurosporine for different time periods. Caspase-3 activation is detected in Western blots by the presence of Caspase-3 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-3, depending on the treatment time points. Pro Caspase-3 is detected at ~32 kDa. Active/cleaved Caspase-3 (large subunit) is detected at ~14-21 kDa as one or more bands.

Simple Western: Caspase-3 Antibody (31A1067) - (Pro and Active) [ABIN2526518] - Simple Western lane view shows a specific band for Caspase 3 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.

Produktdetails anti-Caspase 3 Antikörper

The antibody detects both pro Caspase-3 (~32 kDa) and the large subunit of the active/cleaved form (~14-21 kDa) of Caspase-3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro-domain. It is highly recommended that a maximum sensitivity ECL substrate (Femto sensitive) be used for efficient detection of this antibody in Western blot applications.

Reinigung

Protein G purified

Immunogen

Full-length recombinant human caspase-3 protein was used as immunogen. The antibody recognizes an epitope in the large domain subunit of Caspase-3. As such it will recognize pro Caspase-3 and the large subunit cleavage fragment.

Western blot analysis of Caspase-3 in HeLa cells. Cells were treated with 2 mM staurosporine for different time periods. Caspase-3 activation is detected in Western blots by the presence of Caspase-3 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-3, depending on the treatment time points. Pro Caspase-3 is detected at ~32 kDa. Active/cleaved Caspase-3 (large subunit) is detected at ~14-21 kDa as one or more bands.

Simple Western: Caspase-3 Antibody (31A1067) - (Pro and Active) [ABIN2526518] - Simple Western lane view shows a specific band for Caspase 3 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.

Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [ABIN2526518] - Analysis using the Azide Free version of ABIN2526518. Detection of Lanes 1, 2 and 3 demonstrate the species cross-reactivity of the antibody in human, mouse and rat heart lysate, respectively.

Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [ABIN2526518] - Western Blot Image of anti-Caspase 3 (31A1067). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 5 ug/ml anti-Caspase 3 in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent. Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.

Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [ABIN2526518] - Analysis using the Azide Free version of ABIN2526518. Staining of human breast cancer stained at 4ug/ml. Localization can be cytoplasmic and nuclear. Staining in the nucleus is considered to be an indication of active Caspase-3. In most cell types and mod

Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [ABIN2526518] - Formalin-fixed, paraffin-embedded human breast cancer (top) and normal colon (bottom) stained with Caspase-3 antibody at 4 ug/ml. Localization can be cytoplasmic and nuclear. Staining in the nucleus is considered to be an indication of active Caspase-3. In most cell types and model systems, cells with active Caspase-3 are undergoing apoptosis. Cancer/normal adjacent tissue array was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [ABIN2526518] - Analysis using the Azide Free version of ABIN2526518. Staining of normal colon stained 4ug/ml. Localization can be cytoplasmic and nuclear. Staining in the nucleus is considered to be an indication of active Caspase-3. In most cell types and model systems

Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [ABIN2526518] - Analysis using the Azide Free version of ABIN2526518. Staining of the human thymus after heat-induced antigen retrieval at 5 ug/ml.

Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) - IHC analysis of a formalin fixed paraffin embedded tissue section of human spleen using 1:200 dilution of Caspase-3 antibody (clone 31A1067). The staining was developed with HRP labeled anti-mouse IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Caspase 3 antibody generated primarily a specific cytoplasmic staining in a subset of spleenocytes with some nuclear signal in a few cells.