Hello,
I have troubles labeling tRNA with 5'-gamma-32P, using PNK.
Although incubation of RNA with any of buffers I use does not lead
to a significant degradation, after the labelling I can only see
surprisingly clear bands much below the lowest tRNA (bands about
10-50 bases). A possibility is that only some degradation products
are labelled, and the intact tRNA not, because it is not properly
dephosphorylated. I would be glad if someone could post here a
protocol for RNA dephosphorylation / labelling or point me to a
site where I could find one.
Best regards,
j.
--
----)-\//-///-----------------------------------January-Weiner-3-------
Unix soit qui mal y pense!