Application Information: CD28 antibody [CD28.2]

FACS: Use 5μl for 10^6 cells or 100 μl of whole blood. ICC: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

Concentration

0.1
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Protein A purified

Specifications: CD28 antibody [CD28.2]

Full Name

CD28 molecule

Product Description

Mouse monoclonal [CD28.2] to CD28

Background

Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide-MHC complexes, and the second is delivered by accessory or co-stimulating molecules through their counter-receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.