製品の特性

製品の状態

Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.

保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

アプリケーション

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション

Abreviews

特記事項

WB

Use a concentration of 0.1 - 0.2 µg/ml.

To detect hMCAF/MCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant MCP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect MCP-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9669 is required. This antigen affinity purified antibody, in conjunction with ab9858 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant MCP-1.

IHC-P

Use at an assay dependent concentration.

ICC/IF

Use at an assay dependent concentration. PubMed: 20369226

ターゲット情報

機能

Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

配列類似性

Belongs to the intercrine beta (chemokine CC) family.

翻訳後修飾

Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.

画像

Western blot - Anti-MCP1 antibody (ab9669)

Developed using the ECL technique

Performed under reducing conditions.

HL-60 cells were incubated at 37&degC for 24hrs with vehicle control (0 &microM) and varied concentrations of 2-Arachidonylglycerol (ab120098). Increased expression of MCP1 in HL-60 cells correlates with an increase in 2-Arachidonylglycerol concentration, as described in literature.

Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10&microg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab9669 at 1 &microg/ml and ab8227 at 1 &microg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

ab9669 at 2.5 µg/ml staining human breast invasive ductal carcinoma tissue by IHC-P. The anitbody was incubated for two hours at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended.

The antibody is covered under our Abpromise for six months and is guaranteed to work in IHC-P on human samples . If we cannot resolve the issue you are having with the antibody then I would be happy to either se...

I apologize, but ab73680 is out of stock, and it may not be put back into stock for several months as recent lots have not passed quality control testing. Would you be interested in trying another vial of ab...

At this point, I have not heard from Bio-Connect regarding these issues and I've looked through our system and don't see that they've corresponded with anyone else at Abcam. Have you received any update from...

Thank you for getting back to me and for getting in touch with BioConnect. I will let you know if I don't hear anything from them shortly, and we will figure out the vial of ab9669. I'll also send you a confirmation email after I work everything out wi...