I have encountered a PCR problem that is new to me: A student is having
difficulty with amplification of a plastid DNA locus in the plant
Cimicifuga (Ranunculaceae). I suggested she add a recalcitrant DNA
sample [that had never worked before] to her positive control to test
if the Cimicifuga DNA extraction had a PCR inhibitor. To our surprise,
both the control AND the Cimicifuga locus amplified! We don't want to
add the control DNA to each sample - any thoughts on how to replicate
its effect?
All ideas appreciated.
Aaron Liston
Oregon State University
Martin Goodson wrote:
> hello all,
>> I've heard that PCR on plant genomic DNA has specific diffulties not
> encountered with the DNA of other organisms.