Novel Gene Therapy Control System Regulates Expression of Therapeutic Transgenes

Korean researchers have described a novel control system to regulate the expression of a therapeutic transgene by targeting the passenger strand of a microRNA (miR-122) linked to the transgene. The researchers report that a control system based on targeting the passenger strand of miR-122 rather than the guide strand can regulate expression of an exogenous, therapeutic gene, while not affecting the expression of endogenous genes.

Sung Jin Kim, Chang Ho Lee, and Seong-Wook Lee, Dankook University, Yongin, Republic of Korea, describe the novel gene therapy control system they developed in an article. They compare the results of studies to regulate the expression of a transgene by targeting either the guide or passenger strand of miR-122, and discuss the underlying mechanisms to explain the observed miRNA activity and up- and down-regulation of gene expression.