This assay employs an antibody specific for Rat betaNGF coated on a 96-well plate. Standards and samples are pipetted into the wells and betaNGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat betaNGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of betaNGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

相关性Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons; neurotrophic factor; cutaneous innervation; growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta.

The antibodies in this kit were raised with the mature form of the protein bNGF (aa 122-241). Based on sequence similarities the pro-NGF could possibly be recognized. However, we have not tested the pro-form with this kit so I am afraid we ca...

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The minimum detectable dose of beta-NGF is typically less than 15 pg/ml with this kit. We present this range with the more conservative low point of 49pg/ml to ensure accurate data. Regarding standards, the included stan...

Thank you for contacting Abcam. I have had a reposnse from the lab about the issues you were having with ab100757. Please see their response below: Were the wells with precipitate also “dark blue” before adding the Stop Solution? If so, thi...

Thank you for contacting Abcam. I just wanted to let you know that I am still waiting to hear back from the lab about the problems you have been having with ab100757 and I will contact you again, as soon as I hear back from them. I do appreciate your p...

Thank you for your inquiry. While ab100757 has not been validated with urine (only serum, plasma, and cell culture supernatants), our ELISA kits are very accommodating to many different types of samples so urine along with other body fluids will likel...

While this kit has only been validated with serum, plasma, and cell culture supernatants, it is very accommodating and should work with most
sample types including cell and tissue lysates.
Attached are some general tips to help the customer prepa...