Journal of Medicinal Plantsفصلنامه گياهان داروييJMPMedical Scienceshttp://jmp.ir1admin1684-024022228714888813fajalali139651gregorian201781363online1fulltextenDiversity Evaluation of Trigonella foenum-graecum Populations Using DNA Markers and Phytochemical CharacteristicsبيوتكنولوژيBiotechnologyپژوهشيResearch<a name="OLE_LINK59"></a><a name="OLE_LINK58"><strong>Background:</strong></a> <strong>Fenugreek (<em>Trigonella foenum-graecum </em>L.) is an important medicinal plant that is a source of diosgenin, trigonelline and other valuable components. Fenugreek seeds is used for its pharmaceutical and nutraceutical properties.</strong><br>
<strong>Objective:</strong><strong> The aim of present study was to investigate the genetic diversity within and between different populations of <em>Trigonella foenum-graecum </em>using DNA markers and phytochemical characteristics. </strong><br>
<strong>Methods:</strong><strong> The total genomic DNA was isolated from 12 populations of <em>Trigonella foenum-graecum </em>collected from different regions in Iran. After optimizing PCR, 10 ISSR and 10 SCoT primers were used to amplify the genomic DNA of all genotypes. To investigate genetic distance, cluster analysis based on Jaccard similarity coefficients and UPGMA algorithm calculated for the 12 genotypes. Also, the trigonelline content was determined between 2 populations which had the highest genetic distance.</strong><br>
<strong>Results:</strong><strong> Analysis of banding patterns of 10 SCoT primers revealed 92 polymorphic bands with an average of 9.2 fragments per prime. A total of 79 bands were generated from 10 ISSR primers, out of which 55 (68.21%) were polymorphic bands. The average values of dissimilarities based on the SCoT and ISSR data were 0.60 and 0.44, respectively. Also, shoot trigonelline content was significantly different between genotypes with the highest genetic distance.&nbsp; </strong><br>
<strong>Conclusion:</strong><strong> Our results revealed a high level of genetic variation among fenugreek populations. The findings also indicated that SCoT technique is more informative than ISSR technique for evaluation of genetic diversity and relationships among fenugreek populations. The genotypes with the highest genetic distance based on SCoT data, were noticeably different in respect of trigonelline content.&nbsp; </strong><br>
&nbsp;Trigonella foenum-graecum L., Genetic variation, ISSR, SCoT119132http://jmp.ir/browse.php?a_code=A-10-472-5&slc_lang=en&sid=faHDaneshmand 100319475328460011516100319475328460011516Yes
1- Department of Biotechnology and Plant Breeding, Kermanshah Branch, Islamic Azad University, Kermanshah, IranAR Etminan 100319475328460011517100319475328460011517No1- Department of Biotechnology and Plant Breeding, Kermanshah Branch, Islamic Azad University, Kermanshah, IranA Qaderi 100319475328460011518100319475328460011518No2- Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran