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Description

Applications

Mycoplasma Removal Agent (MRA) eliminates mycoplasma contamination from cell cultures. The cytotoxicity of MRA is low and cell toxicity is rare when used at the recommended concentration. For specific function of any cell, however, it is recommended that the retention of desired cellular characteristics be confirmed after treatment.

Mycoplasma Removal Agent (MRA) from MP offers a highly effective solution for the serious problem of mycoplasma contamination in cell cultures. MRA is a derivative of the quinoline family of antibiotics and eliminates mycoplasma infection (recommended use at a concentration of 0.5 μg/mL) by inhibiting mycoplasma DNA gyrase. MRA is supplied as a ready-to-use solution and each vial can decontaminate up to 25 cell cultures. MRA has been cited by the European Collection of Animal Cell Cultures (Porton Down, U.K.) as being superior to all other current products for the eradication of mycoplasma. Furthermore, cell toxicity is low following treatment at the recommended concentration, as is regrowth of mycoplasma after cellular transfer.

Typical Working Concentration

The recommended concentration for use is 0.5 µg/mL. Concentration may be raised up to 1 µg/mL only when the recommended concentration is ineffective in removing the mycoplasma. For preventative purposes, MRA can be used at 0.1 µg/mL.