Structure

The gene spans approx. 27 kb and contains twelve exons interrupted by eleven introns. The region flanking the 5’ end of the gene lacks a TATA box, but contains many GC’s and also has two CAAT boxes. The gene also may have a binding site for the transcription factor, Sp1, and sequences resembling the binding sites of several other transcription factors. Additionally, there is a 101-bp DNA fragment immediately upstream from the cap site that has promoter activity.

The human ACAT1 gene produces a chimericmRNA through trans-splicing, a process in which separate transcripts from chromosomes 1 and 7 are spliced together. The chimeric mRNA transcript uses two sections to initiate translation: AUG(1397-1399) and GGC(1274-1276). Initiation of the first codon (AUG) results in the translation of a 50-kDa ACAT1, and initiation of the other (GGC) produces another enzymatically active 56-kDa isoform respectively; the 56kDa isoform is naturally present in human cells, including human monocyte-derived macrophages.

Why God Why

(KIM leads CHRIS out of the Club, and into a tiny cubicle, which has in itonly a bed, a table, and a small window overlooking the moonlit city.Later in the night, CHRIS is dressed and standing at the window. KIM isasleep. Outside, Saigon still bustles.)CHRISwhy does Saigon never sleep at night?why does this girl smell of orange trees?how can I feel good when nothing's right?why is she cool when there is no breeze?Vietnamyou don't give answers, do you friend?just questions that don't ever endwhy God? Why today?I'm all through here, on my waythere's nothing left here that I'll misswhy send me now a night like this?who is the girl in this rusty bed?why am I back in a filthy room?why is her voice ringing in my head?why am I high on her cheap perfume?Vietnamhey look I mean you no offensebut why does nothing here make sense?why God? Show your handwhy can't one guy understand?I've been with girls who knew much moreI never felt confused beforewhy me? What's your plan?I can't help her, no one canI liked my mem'ries as they werebut now I'll leave rememb'ring her(CHRIS leaves some money on a table and goes out into the street. CHRISis accosted by Vietnamese who beg for help leaving the country. Hepushes them.)when I went home beforeno one talked of the warwhat they knew from TVdidn't have a thing to do with meI went back and re-uppedsure Saigon is corruptit felt better to behere driving for the embassy'cause here if you can pull a stringa guy like me lives like a kingjust as long as you don't believe anything(CHRIS stops, then goes back into KIM'S room.)why God? Why this face?why such beauty in this place?I liked my mem'ries as they werebut now I'll leave rememb'ring herjust her