The material was found to harbor a bacterial community of 2.38 × 10-8CFU/g dw dominated by Gram-positives with minor instances of Actinobacteria and Gammaproteobacteria. ARISA showed a coherence of bacterial assemblages in different preparation lots of the same year in spite of geographic origin. Enzymatic

activities showed elevated values of β-glucosidase, alkaline phosphatase, chitinase, and esterase. The preparation had no quorum sensing-detectable signal, and no rhizobial nod gene-inducing properties, but displayed a strong auxin-like effect on plants. Enzymatic analyses indicated a bioactive potential in the fertility and nutrient cycling contexts. The IAA activity and microbial degradation products qualify for a possible activity as soil biostimulants.

Of the bacterial species, two dominated 90% of the culture. Half was Bacillus megaterium a plant growth promoting rhizobacteria (PGPR) known to produce Cytokinin. While the other half was Bacillus safensis another PGPR known to produce Auxins.