Genetic Alterations:
BLSW/Ntra carries a spontaneous
mutation initially identified as age related hearing loss 5 (ahl5), which confers hearing impairment. The mutation is located in the Gipc3 gene and is a G>A transition at nucleotide position c.343. This allele is a strain characteristic of BLSW/Ntra.

Homozygous phenotype: Thr ahl5 alle is an amino acid replacement (glycine to arginine)
in the PDZ domain of Gipc3. It causes progressive sensorineural hearing
loss and audiogenic seizures in young adults.

Heterozygous phenotype: The
strain is not available as heterozygous mutation.

Strain of Origin: Black Swiss

Strain genetic background: Black Swiss

Strain Development: Black Swiss mice were initially obtained
from Taconic. By strict brother x sister matings the current F11
population was obtained.

Colony and Husbandry Information

Special Considerations

None.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email
mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU).
A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded
to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain.
(Use the MMRRC Catalog Search to request more than one strain.)

MMRRC Item # - Description

DistributionFee/unit (US $)

Units

Notes

034409-MU-RESUS

Litter recovered from cryo-archive

$2,022.00 / $4,109.00

Non-Profit / For-Profit

Litter

Recovered litter1; additional fees for any special requests.

034409-MU-SPERM

Cryo-preserved spermatozoa

$437.00 / $817.00

Non-Profit / For-Profit

Aliquot

Approximate quantity.2

034409-MU-EMBRYO

Cryo-preserved embryos

$1,038.00 / $2,621.00

Non-Profit / For-Profit

Aliquot

Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the
expense of resuscitating mice from the cryo-archive; you will
receive the resulting litter. The litter will contain at minimum
one mutant carrier; the actual number of animals and the gender
and genotype ratios will vary. (Typically, multiple breeder pairs
can be established from the recovered litter.) Prior to shipment,
the MMRRC will provide information about the animals recovered. If
you anticipate or find that you need to request specific
genotypes, genders or quantities of mice in excess of what is
likely from a resuscitated litter, you may discuss available
options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with
sufficient sperm to recover at least one litter of mice, as per
provided protocols, when performed at the MMRRC facility. The
MMRRC makes no guarantee concerning the success of these
procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient
number of embryos (in one or more vials and based on the transfer
success rate of the MMRRC facility) to transfer to at least two
recipients. The MMRRC makes no guarantee concerning embryo
transfer success experienced in the recipient investigator's
laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the
Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the
Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

The MMRRC is a collaborative effort, funded by grants from DPCPSI of the
NIH.