Optimization of parameters for spectrophotometric determination of two-component analytes using signals ratio method

Granter

University of ZagrebFaculty of Food Technology and Biotechnology

Lower level organizational units

Department of Chemistry and BiochemistryLaboratory for General and Inorganic Chemistry and Electroanalysis

Place

Zagreb

State

Croatia

Scientific field, discipline, subdiscipline

BIOTECHNICAL SCIENCESBiotechnology

Study programme type

university

Study level

graduate

Study programme

Biotechnology; specializations in: Biochemical-Microbiology

Study specialization

Biochemical-Microbiology

Academic title abbreviation

dipl. ing. bioteh.

Genre

master's thesis

Language

Croatian

Defense date

2012-07-16

Parallel abstract (English)

The signals ratio spectrophotometric method was applied in this work for simultaneous quantitative determination of food dyes Sunset Yellow and Azorubine. In order to optimize the signals ratio method, the effect of different parameters on the accuracy of the obtained results was investigated in binary mixtures and semi-real sample and optimal parameters were then applied for determination of the concentration of dyes in a sample of fruit juice. The concentrations obtained for individual dyes in binary synthetic mixtures and real samples were compared with the results obtained using derivative spectroscopy. Results obtained using the ratio of the signal are compared with the derivative method and results showed agreement in the ranging up to 10%.