COX-2 (human) LANCE Ultra TR-FRET Detection Kit, 10,000 Assay Points

The LANCE® Ultra Human COX-2 Detection Kit is designed for detection and quantitation of human COX-2 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

The LANCE® Ultra Human COX-2 Detection Kit is designed for detection and quantitation of human COX-2 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

No-wash steps, no separation steps

TR-FRET technology

Sensitive detection

High reproducibility

Faster time-to-results

Easy automation

96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

COX-2 is a homodimer of 70 kDa subunits that is also known as cyclooxygenase-2, prostaglandin epoxide synthase or prostaglandin G synthase. It is a heme-bearing membrane associated enzyme whose function is to attach an epoxy group to arachidonic acid, creating prostaglandins G or H. This epoxide is then used by other enzymes to generate prostaglandins with oxygen groups on their structure. COX-2 is one of the most important mediators of inflammation since the molecules it generates can activate cells to produce inflammatory cytokines. Also, neurons involved in pain detection bear receptors for these prostaglandins, making it one of the prime target for anti-pain analgesics including the popular aspirin (salycilyc acid), Tylenol™ (acetaminophen) or Advil™ and Motrin™ (ibuprophen).