Sequencing Pol is a Taq polymerase mutant showing an enhanced efficiency for incorporation of ddNTPs. Its capability of incorporating ddNTPs and dNTPs at equal rates makes it the ideal choice for DNA cycle sequencing.
The enzyme replicates DNA at 74°C. It catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction in the presence of magnesium and has a 5'→3' polymerisation-dependent exonuclease replacement activity only.