Interleukin-10 (IL 10) is also known as human cytokine synthesis inhibitory factor (CSIF) and inhibits the synthesis of pro-inflammatory cytokines like IFN-gamma, IL2, IL3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. This protein has effects in immunoregulation and inflammation and down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. Recombinant IL10 protein, fused to His-tag at N-terminus, was expressed in E. coli and was purified by conventional chromatography techniques.

For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

20.94kD

Source

E. coli

Purity

Purified 95% by SDS-PAGE.

Concentration

~0.5mg/ml

Form

Supplied as a liquid in 20mm Tris-HCl buffer, pH 8.0, 20% glycerol.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.