dear mike
> do you have SDS in your buffer? In which case precipitation
no, as far as i know there is no sds in the loading buffer. the buffer
contains:
basic fuchsin, edta, bromophenolblue and formamide. and the samples
loaded, contian the buffers from the autosequencing pcr.
> may be taking place if the rinsing buffer is too cold: use warm buffer?
we rinse with double destilled water, i assume it is at about room
temperature.
> (using warm water or ethanol after the event may be no good because a
> tight plug forms). Mike.
that is an interesting point. do you think that the proteins (especially
taq polymerase) remainings could denaturate and block the syringae?
felipe