Fig. 2. TAT1 depletion sensitizes nocodazole-resistant microtubules to mechanical breakage.
(A to C) Microtubules were imaged in real-time in
siRNA-treated RPE-[EMTB-GFP3] cells after at least
15 min in the presence of 2 mM nocodazole. Projection images were generated to capture microtubules
across the entire cell thickness and to avoid missing
microtubule segments because they left the focal
plane. The yellow lines highlight microtubule behavior, and the red box indicates the first frame
where rupture is clearly detected. Scale bar, 1 mm.
The time series are extracted from movies S5 and
fig. S7. (D) Microtubule breakage events preceded
by buckling were counted in control and TAT1-
depleted RPE-[EMTB-GFP3] cells during the 15- to
80-min period of nocodazole treatment. N = 46
(siControl) and 52 (si TAT1) cells, six independent
experiments. The bar marks the mean. Asterisks
indicate t test significance values; ***P < 10−4.