Opal Polaris 7 Color Manual IHC Detection Kit

Introducing Phenoptics 2.0™: Opal™ Polaris Kits are part of a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image, across the entire digital slide. The method is similar to standard immunohistochemistry (IHC) and is accessible to many laboratories where standard IHC method development is performed. Opal Polaris 7-Color IHC Detection Kits are optimized for whole slide multispectral imaging with the Vectra® Polaris™ Automated Quantitative Pathology Imaging Instrument.

Users should source primary antibodies and other workflow reagents separately. The Opal 7-Color Manual IHC Kit was optimized for use on the Vectra® Polaris system. Kits contain enough reagents to stain 50-slides at the recommended 1:100 dilution ratio.

What is Opal? Opal Multiplex IHC Kits make multiplex methods accessible to researchers who works with standard immunohistochemistry (IHC). The Opal method allows use of any number of unlabeled primary antibodies from the same species in multiplexed tissue assays, with no fear of cross-reactivity. Antibodies for simultaneous IHC may now be selected based on performance, rather than species. The method works with formalin fixed paraffin-embedded (FFPE) tissue and is compatible with the standard research IHC workflow in your lab.

Opal provides researchers with the tools to interrogate multiple pathways while retaining context provided by tissue images. This approach provides information not available from alternative techniques like flow cytometry or analysis of single markers in serial sections.

Opal will enable you to:

Measure up to six tissue biomarkers at once

Use the best primary antibodies together in multiplex panel, with no species-based crosstalk

Retain spatial cellular context that is lost in flow cytometry

Confirm single cell co-expression for many biomarkers in one tissue section