Chromosome Resolution Additive

Chromosome Resolution Additive

CRA (Chromosome Resolution Additive) is a ProCell Cytogenetics Reagent that prevents chromosome contraction and encourages chromosome elongation by loosening the chemical bonding within the supercoiled structure of the chromosome.

Chromosome Resolution Additive (CRA) is a newly discovered commercial formulation that consistently prevents chromosome contraction and encourages chromosome elongation by loosening the chemical bonding within the supercoiled structure of the chromosome. The result is prophase quality chromosomes. It was developed especially for tissues resistant to existing prophase techniques, such as bone marrows and solid tumour samples. CRA contains no ethidium bromide or thymidine.

CRA is a simple-to-use product that increases chromosome resolution and is safer than ethidium bromide. A working solution of CRA is simply
added to the culture up to 90 minutes prior to metaphase arrest.
This product has been shown to increase QA band level on different cytogenetics sample types. CRA is a colchicine antagonist, this means that it reduces the chromosome shortening effect of colchicine. CRA can also be used alongside ethidium bromide for extra chromosome length.