Research Tools

PRODUCTS

His tag Antibody, Agarose

Catalog #

Pack Size

Price(USD)

ICP1310

10 mg

$950.00

Quantity:

Product Description

The His-tag (HHHHHH) antibody is immobilized to beaded agarose via amide linkages. The product could be utilized as an affinity matrix for rapid immunoprecipitation, isolation, purification and enrichment of recombinant proteins expressing the His-tag.

Approximately 0.1 µmole/mL or more than 5 mg/mL of 50kDa His-tag proteins

Applications

IP, IAC for rapid isolation, purification snf enrichment of peptides or proteins with the His-tag from cell lysate mixtures.

Scientific Description

The His tag (HHHHHH) has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the His tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. Affinity chromatography is one of the most efficient and rapid methods to enrich and purify His-tag proteins.

Storage & Stability

Product is stable for at least 30 days at room temperature. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.