Product Details anti-Caspase 4 Antibody

Product Details anti-Caspase 4 Antibody

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Immunogen

A 16 amino acid peptide from human Caspase-4 amino terminus

Isotype

IgG

Specificity

This antibody recognises an epitope within the N-terminal region (NT) of Caspase-4, otherwise known as ICH-2. This antibody detects a band of approximately 34 kDa in Ramos cell lysates (predicted precursor MWT 31 kDa).

Purification

Affinity Chromatography

Target Details Caspase 4

Target Details Caspase 4

Caspase-4, a member of the Caspase-1 subfamily of cysteine proteases, exists as an inactive pro-enzyme which undergoes proteolytic cleavage of its p30 precursor form, into smaller p20 and p10 subunits. The cellular localisation of Caspase-4 on the Endoplasmic Reticulum (ER) membrane, has resulted in this protein becoming a focus of studies in which dysfunction or stress to the ER membrane is implicated, such as Alzheimers disease and Ischemia (1, 3) and confirms the involvement of Caspase-4 as an instigator of cellular apoptosis.Synonyms: CASP4, ICE(rel)-II, ICH2, Protease ICH-2, Protease TX

Application Details

Application Details

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Application Notes

Western Blot: 1.0 μg/mL. Immunohistochemistry on paraffin sections: 2.0 μg/mL, This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining, This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Control: Spleen tissue. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.