Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

adenosquamous carcinoma

Age

73 years

Gender

male

Ethnicity

Caucasian

Applications

This cell line is a suitable transfection host.

Storage Conditions

liquid nitrogen vapor phase

Karyotype

modal number = 71; range = 65 to 75.This is a near triploid human cell line. The modal chromosome number was 71 although cells with 70 chromosomes occurred frequently. The frequency of higher ploidies was 2.9%.; Over 11 marker chromosomes were common to all cells, and at least 6 others were found in some cells. Among the common markers were: del(1)(p22), i(3q), i(6p), i(7p), t(7p17q), 14q+ and five others.; Of these, del(1) and i(7p) were generally double [occasionally triple for del(1)]. Constantly, there were 2 to 3 minute chromosomes that were about 1/2 the size of a G group chromosome.; Structurally normal N17 had only a single copy per cell, whereas N15 had four copies and N20 and N21 had four or more copies. The X chromosome was paired, but the Y was not found by fluorescence microscopy.

Images

Derivation

The NCI-H596 cell line was derived by A.F. Gazdar and H. Oie in 1983 from a tumor mass in the chest wall of a patient with adenosquamous carcinoma of the lung.

The specimen was obtained prior to therapy.

Clinical Data

73 years

Caucasian

male

The NCI-H596 cell line was derived by A.F. Gazdar and H. Oie in 1983 from a tumor mass in the chest wall of a patient with adenosquamous carcinoma of the lung.

Tumorigenic

Yes

Effects

Yes, in nude mice

Comments

The cells express easily detectable levels of p53 mRNA (comparable to levels found in normal lung), and exhibit no gross structural DNA abnormalities.

The cells stain positively for keratin and vimentin, but are negative for neurofilament triplet protein.

The cells express multiple markers of squamous differentiation, including cross linked envelope formation, transglutaminase activity and production of involucrin.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied.

Any proposed commercial use of the these cells, or their products, must first be negotiated with the National Cancer Institute (NCI). For further information, please contact NCI’s Technology Transfer Center at NCI_TTC_Contact@mail.nih.gov or by phone at (240)-276-5514.