BPN450: A Microsystem for Sensing and Patterning Oxidative Microgradients During Cell Culture

Project ID

BPN450

Website

Start Date

Thu 2008-Jan-31 16:25:45

Last Updated

Tue 2009-Aug-11 15:28:52

Abstract

We present a microsystem capable of electrochemically patterning dissolved oxygen gradients during
cell cultures. Multiple electrodes in an array each generate distinct amounts of dissolved oxygen
via electrolysis; these different sources superimpose to generate one- and two-dimensional
microgradient profiles not possible with other methods. We believe this is the first technology
that enables researchers to pattern localized oxygen doses and program arbitrary oxygen gradients
with microscale resolution during cell culture.