CD28:B7-2[Biotinylated] Inhibitor Screening Assay Kit

Catalog #: 72062

Size: 96 reactions

$1,040

Background:

The activation of naïve T cells requires two signals; the specific T cell receptor recognition of MHC/Antigen on the surface of the antigen-presenting cell (APC), and the binding of B7-2 (CD86) ligand on the APC with the CD28 receptor on the T cell surface. CD28:B7-2 interaction is an important drug target for the regulation of T cells involved in autoimmunity, inflammation, tumor recognition, and immune tolerance.

Description:

The CD28:B7-2[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD28:B7-2 signaling. The key to this kit is the high sensitivity of detection of biotin-labeled B7-2 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD28 is coated on a 96-well plate. Next, B7-2 is incubated with CD28 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.