This 5-filter "Sedat" set is ideal for high-speed sequential imaging FITC and Texas Red with high brightness, extremely low crosstalk, and superb signal-to-noise ratio. It is also very popular for imaging GFP and mCherry. It is comprised of two single-band exciters and two single-band emitters, along with a dual-band beamsplitter, and thus works best when used with both exciter and emitter filter wheels.

Optical Specifications

Specification

Value

Blocking

BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications

Specification

Value

Microscope Compatibility

BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.

Optical Damage Rating

Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.