Figure 1. R&D Systems Human Cytokine Array, Panel A ( Catalog # ARY005) utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 36 cytokines, chemokines, and acute phase proteins in a single sample. Peripheral blood mononuclear cells treated with PHA for 24 hours were mixed with a cocktail of biotinylated detection antibodies, and then incubated with the Human Cytokine Array. The array was then incubated with streptavidin-horseradish peroxidase followed by chemiluminescent detection. After detection, the array data were quantitated to generate a protein profile (histogram). The table shows the analytes detected and their location on the membrane.