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Leading the queries were Ruth Davidson, Leader of the Scottish Conservative and Unionist Party, who tweeted: 'Tom Cruise's face. I don't know what he's doing to it, but he's beginning to look like a late-90s era Sandi Toksvig. #EEBAFTAs.'

Scottish broadcaster Muriel Gray weighed in: 'Tragedy of Tom Cruise having ruined his lovely face, that was ageing beautifully, is that it's gone for ever now like the Bamiyan Buddahs.'

Questions? Many viewers of the BAFTAs took to Twitter to speculate about Tom's appearance

Dapper: The 53-year-old looked smart in a black Dolce & Gabbana suit as he made a surprise guest appearance at the glamorous London ceremony

Man of the hour: Tom points to the big winner Alejandro G Iñárritu, whose film The Revenant picked up five major accolades

One Twitter user @hwallop wrote: 'What has Tom Cruise done to his face? Scarier than the bear in Revenant.'

Another @lisab7us tweeted: 'I see Tom Cruise has gone and bought himself a new face. #BAFTA.'

The furore on the micro-blogging site came as Tom was presenting the Best Film Award to the cast and crew behind The Revenant.

Leonardo DiCaprio and Alejandro González Iñárritu were among those on stage accepting the BAFTA for Best Film following the duo's win for Best Actor and Best Director respectively.

Furore: Fans were quick to tweet about what they thought the actor had done

Under the microsope: Many on Twitter thought the A-lister's face looked 'frozen' and 'inflated' as he posed with Leonardo DiCaprio and Alejandro

Introducing Tom, host Stephen Fry joked that he had found him sleeping backstage, having never left the building since attending the 2015 BAFTAs.

Tom was previously asked about surgery rumours back in a 2012 interview with Playboy magazine and he denied having gone under the knife.

He said: 'I haven’t, and I never would.'

Congratulations: Tom and Leonardo chat backstage following the award presentation

Cosmetic surgeon Mr Sultan Hassan FRCS(Plast) of Elite Surgical - who has not treated the actor - told the MailOnline: 'I suspect he has had botulinum toxin injections to his glabellar, forehead and crow’s feet areas within the last few weeks. I personally feel his injector has used far too much toxin resulting in a rather unnatural shiny look.

'If you look carefully at his eyebrow positions they look heavy and rather flat, low and wider apart. You could argue that this is a rather poor result. In addition his cheek bone, cheeks and jawline looks filled out, which in the absence of general weight gain is likely due to the use of a collagen filler to plump these areas out - just look carefully at his tear troughs and you can see they are filled out.'

Miles Berry MS, FRCS consultant plastic surgeon of Cosmeticsurgery-partners.co.uk, remarked: 'I would agree that Botox is likely to produce the smooth, unlined forehead. The cheeks are looking a tad more full too so dermal fillers would be high on the list too.

'As to the "sheen", the most likely culprit would be some form of resurfacing, probably a chemical peel.'

MailOnline has contacted Tom's rep for comment.

Waiting game: Tom waiting backstage with the BAFTA before Best Film before going on stage to announce the winner

Through the years: The actor at a LA Galaxy football game in 2012 (left) and the LA premiere of The Last Samurai in 2003 (right)