Application Information: TCP1 alpha antibody [4E215]

ICC: Use at a concentration of 5 μg/ml. IP: Use at a concentration of 5 μg/ml. WB: Use at a concentration of 1 [ECL] or 2 [Colorimetric] μg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 67 kDa). Optimal dilutions/concentrations should be determined by the end user.

Form Supplied

Liquid

Concentration

0.87
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Protein G purified

Specifications: TCP1 alpha antibody [4E215]

Full Name

t-complex protein 1

Product Description

Rat monoclonal [4E215] to TCP1 alpha

Background

T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55.The TCP 1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.

This antibody recognizes other proteins, most notably the p102B' COP subunit of Golgi coatomer. CTA-123 binds protein A and is useful for native precipitations of coatomer and CTT. This antibody does not react with human Hsp60 protein. This antibody also detects a molecular mass band of approximately 92kD on immunoblots.