Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Details

Applications

Approved:

Immunofluorescence (1:5000)

Western blot (1:10000)

ELISA (1:20000)

Specificity and Use

Immunogen:

Rat IgG antibody was raised against rat IgG whole molecule.

Specificity:

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins. This antibody will react with heavy chains of rat IgG and with light chains of most rat immunoglobulins.

Usage:

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

Restrictions:

For research use only.

About Rat IgG

Description: Representing approximately 75% of serum antibodies, IgG is the most common type of antibody found in the circulation. IgG molecules are created and released by plasma B cells. IgG antibodies are large molecules of about 150 kDa made of four peptide chains. It contains two identical class gamma heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The two heavy chains are linked to each other and to a light chain each by disulfide bonds.