Small Genome Sequencing

Small genome sequencing using an Illumina Miseq. Genomes are enzymatically fragmented and “barcoded”. Instrument yields 22-25 million reads. Great for bacterial and viral genomes. Can also be used for PCR products or DNA fragments greater than 300 bp.

The study was supported in part by the Center for Microbial Pathogenesis and Host Inflammatory Responses grant P20GM103625 through the NIH National Institute of General Medical Sciences Centers of Biomedical Research Excellence. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.