Abstract：The invention discloses an adhesive bandage type artificial active tissue constructed by using a culture solution without serum or bovine pituitary extracts and a construction method of the adhesive bandage type artificial active tissue. A cell culture solution without the serum or bovine pituitary extracts is adopted, and a biological film carrier is specially treated to ensure that a niche suitable for cell interaction is formed on a biological film, and promote human fibroblasts and epidermal cells to give play to self potentials, proliferate, disintegrate and excrete growth factors, so that an artificial tissue with active cells can be formed on the biological film in 4-7 days. The artificial tissue is high in cell activity and strong in division and migration ability, can home back to wound surfaces, and can promote tissue regeneration by serving as seed cells for curing burns and chronic ulcer. The adhesive bandage type artificial active tissue disclosed by the invention completely avoids immunological rejection of animal derived products including serum, pituitary extracts and collagen and the risk of infection of viruses transmitted to humans from animals, and a thin cell tissue taking the biological film as a scaffold and an infection barrier is formed, so that the adhesive bandage type artificial active tissue with biological safety is truly realized.