An Israeli man was killed and two others wounded after a Palestinian fired shots at a family vehicle Monday evening on Route 35 near the Tarkumia checkpoint west of Hebron, police said.

A 40-year-old man was pronounced dead after attempts at resuscitation, while a 28-year-old woman was in moderate condition after suffering injuries to her upper body, according to Magen David Adom. She was taken to Shaare Zedek Medical Center in Jerusalem for further treatment.

A nine-year-old boy suffered light wounds from shrapnel in his chest. He was taken to Hadassah University Medical Center at Ein Kerem.

The victims were in their vehicle at the time of the shooting. The assailant fired at three different vehicles on Route 35, according to MDA.

An initial IDF investigation revealed that at least one armed gunman approached the road on foot, and shot at the first car that passed from only a few meters away.

The shooter then ran to another point on the road and continued to shoot at Israeli vehicles. The shooter then fled the scene.

An eyewitness told Channel 2 that a man wearing a helmet on the side of the road all of a sudden opened fire on passing cars with a AK-47 rifle.

IDF officials said there was no military intelligence that hinted at an attack on Jews on the eve of Passover.

MK Orit Struck (Bayit Yehudi) told Army Radio that she has no doubt the attack was "a direct result of releasing [Palestinian] prisoners that help turn the wheels of terror...releasing prisoners leads to terrorist attacks."

The Islamic Jihad released a statement commending the attack as "a natural response to Israel's crimes." The terrorist group did not claim responsibility for the attack.

IDF security forces, police, and Border Police were carrying out a search for the shooters.