Re: Proliferation in rat

From:

John Bliss <bliss@zymed.com>

Dear Kenneth:
BrdU is a highly specific and sensitive marker for cell proliferation-however,
you need to introduce the BrdU labeling reagent into the rat before it is
sacrificed.
PCNA is one of the original cell proliferation markers and the PC10 clone
antibody works in almost every species including rat.
Zymed has antibodies to both BrdU and PCNA, and also BrdU and PCNA kits that
are designed to work in tissue from any species. Please contact our Swedish
distributor for more information. Thank you.
Regards,
John Bliss
--
Kenneth Wester wrote:
> Hi All!
>
> Is there anyone who has experience from and/or suggestions on how to study
> proliferation in rat tissue, using immunohistochemistry. One problem is the
> crossreactivity between mouse and rat. Another is to find antibodies
> recognizing the "rat-epitope" for the respective proliferatio associated
> protein.
>
> Kenneth Wester
>
> Kenneth Wester, Ph.D. phone: +46 (0)18 611 02 06
>
> Dept. of Genetics & Pathology fax: +46 (0)18 471 3432
> The Rudbeck laboratory
> Uppsala University
> Dag Hammarskjöldsväg 20
> S-752 37 Uppsala, Sweden