Oil pollution has become a global issue because of its severe ecological impact and destruction. Bioremediation is proved to be an effective process to restore the oil polluted environments. Bacteria from oil contaminated environment always have the abilities for degradation of hydrocarbons and are thought to be useful in bioremediation of oil pollution. Amycolicicoccus subflavus DQS-9A1T, an actinomycete, is a type strain of newly published novel species in the novel genus Amycolicicoccus, isolated from the crude oil-polluted saline soil in China. The complete genome sequence of Amycolicicoccus subflavus DQS-9A1 will provide new strategies for bioremediation of oil contaminated environment.