Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

ELISA

RIA

IHC-P

Application notes

ELISA: Use at an assay dependant concentration.
IHC-P: Use at an assay dependent dilution.
RIA: Use at an assay dependant concentration.
Nanowires array: 1/50 - 1/500.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

Images

Human normal prostate. Staining is localised as cytoplasmic and secreted. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system, at room temperature: sections were rehydrated and antigen retrieved with buffers (citrate pH 6.1) in a PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

I am glad you found the reference useful, I am sorry it took a little while to get to you. Unfortunately, the experiment you are planning would not be covered by the Abpromise guarantee. This is because we have not ou...

All of the antibodies you have chosen are appropriate for the experiments you are hoping to perform. All of the antibodies have been raised against the human protein but the cross reactivity with the mouse protein...

Thank you for your enquiry.
The affinity constant was determined by RIA. In brief, according to the lab that performed this characterization:
"Dilution series of antibody ab403 has been prepared and all dilutions have been incubated with 125 I ...

Thank you for your patience in this matter.
I have received a reply from the originator of ab403 (PSA antibody). The pI value of this product is 6,0 - 6,7. The assay method is PhastSystem, PhastGel IEF 3-9.
I hope this information is useful for...