Interpretive Summary: The blacklegged tick Ixodes scapularis Say, is the principal vector of Lyme disease in the Eastern U.S. Substances from external glands on the legs of white-tailed deer, primary host of adult black-legged ticks, aid ticks in finding optimal vantage points where they can wait for hosts. Responses of I. scapularis and lone start ticks, Amblyomma americanum, to substances from interdigital glands on deer's feed were evaluated in laboratory behavioral trials. Adult I. scapularis of both sexes and male A. americanum preferentially came to rest on capillary tubes treated with substances from interdigital glands from fore and hind legs of deer, whereas I. scapularis nymphs and A. americanun females did not show such an arrestant response. These data are of interest to researchers in that they show that ticks can probably locate deer trails or areas frequented by deer and wait there for hosts. It might be possible to identify chemicals in the gland secretions that could be used to mislead ticks from genuine deer trails and reduce the likelihood of their obtaining the blood meal essential to their survival.