pCDCAR1 GPC3 h(b2cζ), T(CAR-T-2-L342-CZ)

The vector of anti-GPC3 chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target human GPC3. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-GPC3 antibody linked to CD3-zeta and b2c signaling domains. And the vector product was designed for the treatment of hepatocellular carcinoma (HCC).

Details

Target

Glypican-3 (GPC3)

Target Species

Human

Targeting Cell Type

T cell

Targeting Diseases

Hepatocellular Carcinoma (HCC)

Generation

Second

Vector Name

pCDCAR1

Vector Length

8kb

Vector Type

Lentiviral

Receptor Construction

scFv-b2c-CD3ζ

Discription of Signaling Cassetes

b2cThe b2c protein belongs to high-sulphur keratin family which comprises at least three proteins, B2A, B2B and B2C. And b2c in chimeric antigen receptorsvcqn improve T-cell effector functions and enhance anti-tumor activity.CD3ζCD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta,which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

Antibody Clone

9C2

Antibody Host

Mouse

Target

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.