Mr. G. Morley wrote:
>> A colleague of mine is having problems running her
>> protein gels.
>> Although her 8% acrylamide sds gels dry down fine (normaly
>> within 2-3hrs) her 10% gels are cracking on the gel dryer.
>> She is pre-wetting her whatman and drying down at 80 degrees
>> centigrade...
>> I seem to remember something about higher percentage gels
>> requiring either soaking, or the addition, of gelatin but
>> I am not sure...also I seem to recall the drying temperature
>> may be important - I used to dry down at 65 degrees c.
>> If anyone has any ideas we would be much obligued to hear
>> from you!
>> Thanks in advance...
>> Gary Morley
>>gmorley at rpms.ac.uk
Two things that may help here, one is check the vacuum source, BioRad
says a water aspirator is insufficient and call for a vacuum of 29 torr
(~28" Hg) or better. The other thing to do is to try soaking the gels
in a 3% glycerol solution before drying.
Hope it helps and happy hunting.
>>>>>>--------------------------->
Ron
Tate
Lab of Franklin Leach
Dept. of Biochem. & Molecular Biology
Oklahoma State University
rtate at bmb-fs1.biochem.okstate.edu
(405) 744-9326
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