Leibovitz's L-15 Medium

L-15 Medium (Leibovitz) was originally formulated for use in carbon dioxide (CO2) free systems requiring sodium bicarbonate. L-15 is buffered by the use of free basic amino acids, phosphate buffers and higher levels of galactose and sodium pyruvate to help maintain physiological pH control. L-15 has been shown to work for human tumor cells and embryonic cells and for established cell lines such as HeLa and Hep-2.