Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures. Recombinant murine SCF is an 18.3 kDa protein containing 164 amino acid residues.

Species

Mouse

Expression System

E. coli

Applications

Apuri, Blocking, ELISA, Functional Assay

Application Note

The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is <10 ng/ml, corresponding to a specific activity of >1 x10^5 units/mg.

Predicted Target Size(KDa)

18.3 kDa(note)

Form Supplied

Lyophilized powder

Purification Note

Purity was assessed by SDS-PAGE (≥98%) and by HPLC (≥98%).

Storage Buffer

10 mM Sodium Phosphate, pH 6.5

Storage Instruction

We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Notes

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.