Drosophila melanogaster Testes cDNA (NIH)

Drosophila melanogaster Testes cDNA (NIH)

The NIH Drosophila cDNA library was constructed by Brian Oliver at the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), part of the National Institutes of Health (NIH) at Bethesda, MD, USA.

The NIH Drospohila cDNA library poly (A) + RNA was prepared from dissected testes from 1-5 day old y w [67cl] Drosophila melanogaster flies, after removal of the paragonia and inclusion of the ejaculatory ducts and seminal vesicles. The size fractionated cDNA (1-6kb) was directionally cloned in the Stratagene Uni-Zap XR vector using EcoRI and XhoI restriction sites. pBluescript SK (-) is produced after excision. The host strain is E.coli SOLR.

Protocols / Clone Handling

Individual clones

References

References & Acknowledgments

Dr. B. Oliver at the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health (NIH), Bethesda, MD, USA.