AccuBead™ Silica Coated Magnetic Bead

Spherical-shaped magnetic beads for superior results

Many research institutes and industries are applying magnetic beads into their applications. They use this material either for cell-based experiments or nucleic acid purification for disease monitoring as well as developing treatments (among other uses). Bioneer's magnetic beads have an average diameter of 1.29 um with a range of 1~5 um diameter and can be coated with
a variety of functional groups. The functional groups allow binding of, for example, DNA, RNA, protein or specific cells.
By using an external magnetic field, the magnetic beads can be separated with their bound products for isolation and purification.

Features and Benefits

Shape and Uniformity: AccuBead™ s completely spherical unlike most other magnetic beads. The average particle size distribution is comparatively uniform, and the smooth surface eliminates carryover of impurities common to rough-surfaced beads.

Efficiency:
Because the silica magnetic beads are spherical and uniform, DNA/RNA or protein purification can be performed with
outstanding efficiency and reproducibility.
The magnetic property of the bead allows an external magnetic field to be applied for quick separation.

Reproducibility:
Each batch is produced under strict quality controls. Errors that commonly occur during mass production are eliminated during
the individual packaging process. Bioneer's current batch processing system allows for the production of more accurate and reproducible end-product yield.

Size Distribution and Zeta Potential Diagram of Magnetic Beads

Figure 3. Size distribution diagram and zeta potential of silica coated magnetic beads.
The average size of magnetic beads is approximately 1.56 µm and zeta potential is -39.8 mV due to the hydroxyl group on the surface of the silica.

Magnetic Beads are a highly efficient tool for DNA isolation

DNA/RNA affinity to magnetic beads (uniform-dispersed super-paramagnetic particles) is used for separation. Magnetic beads are mixed with naked DNA/RNA in high-salt-containing buffer. DNA/RNA is bound to magnetic beads via a salt bridge. By magnetic separation, highly enriched DNA/RNA preparations are obtained in a matter of minutes. Magnetic beads are highly suitable for automation since it requires no centrifugation or vacuum filtration procedures.

First, subject the biological samples to a lysis reaction and place the magnetic beads in the mixture to combine the free DNA together with magnetic beads. Fix magnetic beads with an external magnetic field to wash the solution and then elute
the DNA or RNA from the magnetic beads. Using magnetic beads to for purification is much faster than other methods such as gravity columns or centrifugation.