While I have no objections to using 10-11 day old embryonated chicken eggs to
grow Sendai, I would like to know if any tissue culture systems/cells would
serve just as well, or at least satisfactorily. So far the literature hasn't
pointed me to any particular system, though it seems to indirectly hint at
Monkey kidney cell lines. CHO (Chinese Hamster Ovary) cells seem to resist
cell fusion when incubated with VSV, would an analogous culture system serve to
grow Sendai? Pointers, insights, references welcome; many thanks in advance.
Gratefully,
Soumit K. Basu
--
Soumit K. Basu | "Never send to know for whom the bell tolls--
Dept. of Biomedical Engineering | it tolls for me damn it."
Johns Hopkins University | -- My variation on John Donne
E-mail: sbasu at nye.wbme.jhu.edu | "Iudex bonus rem non verba spectat"