Mononuclear cells (MNCs) are composed of lymphocytes (T cells, B cells and NK cells), monocytes, dendritic cells and stem/progenitor cells. These cells are critical components of the immune system and play key roles in the humoral and cell-mediated immune responses. Mononuclear cells are commonly used in research and clinical applications in the areas of immunology, infectious disease, hematological malignancies, vaccine development, transplant therapy, and high-throughput screening.

Bone marrow MNCs are separated from whole bone marrow using density gradient centrifugation. The highly pure population of MNCs is ideal for further isolations of specific cell types within the mononuclear fraction.