Protein purification

FBMM has a highly developed infrastructure for the purification of recombinant proteins. The facility hosts several purification systems (Table 1) as well as a dedicated lab for protein purification (F205), which also includes a Nanodrop 1000 spectrophotometer, gel documentation device ChemiDocTM XRS+( Bio-Rad) and other devices related to protein purification. Many devices, especially those locating in the protein purification lab, are also available to non-academic users (Table 1). For the academic users we can provide help for: i) planning your protein purification protocol, ii) running your experiments, and iii) analyzing your results (for a basic price or via collaboration). For regular or large-scale purifications research groups must provide required consumables by themselves. Some consumables, like columns, certain column material and SDS PAGE system, we can only provide for test runs. For the commercial users the devices will be provided using an hour- or daily-based rent depending on the device. Weekly or monthly “package offers” for a set of specific devices including working place in the F205 laboratory are also provided if needed.

Table 1. Protein purification units in FBMM. All systems except Bio-Rad Biologic LP are operated via a computer, and include monitors detecting UV and conductivity values. All systems are also connected to fraction collectors. The devices marked by a star are also available to outside users.

instrument

temperature (range)

laboratory

special detectors

person-in-charge of the device

*Äkta Explorer

RT

F205

Hongmin Tu

*Äkta Purifier

10°C-RT

F225

SLS

RI

Ville Ratas

Rajaram Venkatesan

*Äkta Purifier

RT

F205

Kristian Koski

*Shimadzu HPLC purification package

4°C-10°C

F205

SLS

fluorescence

RI

Rahul Nenakar

Kristian Koski

Ville Ratas

*Bio-RadBiologic DuoFlow

4°C-10°C

F225

Shiv Kumar Sah-Teli

Bio-Rad Biologic DuoFlow

4°C-10°C

F224

Matti Myllykoski

Bio-RadBiologic DuoFlow

4°C-10°C

F124

Teemu Haikarainen

Bio-Rad Biologic DuoFlow

RT

F124

Teemu Haikarainen

Bio-Rad Biologic DuoFlow

4°C-10°C

L041

Henni Piirainen

*Bio-Rad, Biologic, LP

4°C-10°C

F205

only UV

Rajaram Venkatesan

Shimadzu-HPLC in F205. Operated in a cold cabin.

Äkta Purifier in F205. Operated at room temperature.

The starting point for these projects will be a planning meeting with Kristian Koski and Ville Ratas.