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Abstract

We describe a new ultrahigh speed Spectral OCT instrument making use of a CMOS camera and demonstrate high quality in vivo imaging of the anterior segment of the human eye. The high flexibility of the designed imaging system allows a wide range of imaging protocols. Two- and three-dimensional high quality OCT images of the cornea, the anterior chamber and the crystalline lens are presented. A high acquisition rate, up to 135,000 A-scans/second enables three-dimensional reconstruction of the anterior segment during lenticular accommodation, blinking and pupillary reaction to light stimulus. We demonstrate OCT tomographic real time imaging of the lens dynamics during accommodation and high quality OCT cross-sectional images of the entire anterior segment of the eye from the cornea up to posterior part of the crystalline lens.

Figures (14)

Sensitivity as a function of the reflectivity of the reference mirror for SOCT systems with CCD (a) and CMOS camera (b) measured for exposure time Texp = 40μs and optical power on the sample P = 1 mW. Theoretical curve (solid line) is fitted to the experimental points using three independent parameters: ρ, Ath, Aex. Each parameter was used to calculate corresponding noise contribution. Dashed lines represent hypothetical situations of sensitivity affected by only one noise component.

Cross-sectional images of the crystalline lens obtained with different single line exposure times: 15 μs (panel a), 33 μs (panel b), 43 μs (panel c). The two dimensional data consists of 10,000×4096 pixels in all three cases.

Cross-sectional image of the crystalline lens: single line exposure time 70 μs, transverse scanning density 15,000 A-scans per 9 mm; 4096 pixels in each A-scan. The red rectangle in panel (a) indicates the location of the magnified region shown in panel b. The capsule CP, nucleus N and cortex CR are visible.