Freshwater crustaceans could be the key to understanding how the chytrid fungus persists in the ecosystem long after the last amphibian is gone.

Scientists have found a new culprit in spreading the disease that's been driving the world's frogs to the brink of extinction: crayfish.

In the last few decades, the disease caused by the chytrid fungus has been a disaster for frogs and other amphibians. More than 300 species are nearly extinct because of it. Many probably have gone extinct, but it can be difficult to know for sure when a tiny, rare species disappears from the face of the Earth. (Related photos: "Ten Most Wanted 'Extinct' Amphibians.")

"This pathogen is bad news. It's worse news than any other pathogen in the history of life on Earth as far as we know it," says Vance Vredenburg, a conservation biologist at San Francisco State University who studies frogs but did not work on the new study.

The chytrid fungus was only discovered in the late 1990s. Since then, scientists have been scrambling to figure out how it spreads and how it works.

One of the biggest mysteries is how chytrid can persist in a frogless pond. Researchers saw it happen many times and were perplexed: If all of a pond's amphibians were wiped out, and a few frogs or salamanders came back and recolonized the pond, they would also die—even though there were no amphibians in the pond to harbor the disease. (Learn about vanishing amphibians.)

One possible reason is that chytrid infects other animals. For a study published today in Proceedings of the National Academy of Sciences, Taegan McMahon, a graduate student in ecology at the University of South Florida in Tampa, looked at some possible suspects and focused on crayfish, those lobsterlike crustaceans living in freshwater. They seemed like a good possibility because they're widespread and because their bodies have a lot of keratin, a protein the fungus attacks.

In the lab, McMahon exposed crayfish to the disease and they got sick. More than a third died within seven weeks, and most of the survivors were carrying the fungus. She also put infected crayfish in the water with tadpoles—separated by mesh, so the crustaceans wouldn't eat the baby frogs—and the tadpoles got infected. When McMahon and her colleagues checked out wetlands in Louisiana and Colorado, they also found infected crayfish.

That means crayfish can probably act as a reservoir for the disease. The fungus seems to be able to dine on crayfish then leap back to amphibians when it gets a chance. No one knows for sure where the fungus originally came from or why it's been such a problem in recent decades, but this research suggests one way that it could have been spread. Crayfish are sometimes moved from pond to pond as fish bait and are sold around the world as food and aquarium pets. (Related photos: "New Giant 'Bearded' Crayfish Species.")

The study doesn't answer every last question about the disease. For one thing, crayfish are common, but they aren't everywhere; there are no crayfish in some of the places where frogs have been hardest hit, Vredenburg says. But, he says, the new research shows that "we need to start looking a little more broadly at other potential hosts."

many fungi are ubiquitous, and it could be that this species is easily transported via air currents. all microbes, but fungi, in particular, may possess genes that increase their potential for pathogenicity. we call these genes virulence factors. amphibian physiology may be increasing the potential for this fungal species to become a pathogen. a good question would be are amphibians the terminal host for this fungal species?