Real-Time qPCR Reagents and Kits

rtStar™ First-Strand cDNA Synthesis Products

Arraystar's rtStar™ series contains 4 types of products, commendably meeting the needs of the market. These products are suitable for reverse transcribing total RNAs, tRNAs, miRNAs and other small RNAs, respectively. These kits feature high efficiency in first strand cDNA synthesis. And the cDNA products can be directly used in the qPCR detection and quantification.

To meet the needs of the market, Arraystar develops 4 types of first-strand cDNA synthesis kit. The rtStar™ First-Strand cDNA Synthesis Kit is designed to create cDNA libraries from total RNAs for qPCR. Random primers andoligo(dT)18 are provided to satisfy customers' choice. Both rtStar™ First-Strand cDNA Synthesis Kit (3'adaptor) and rtStar™ First-Strand cDNA Synthesis Kit (5'and 3'adaptor) could be used for converting small RNAs into cDNA templates. The latter one is extremely suitable for small RNAs that are too short to design qPCR primers. On account of diverse modifications, cDNA products of tRNAs always contain strong transcriptional pauses. To figure out this problem, rtStar™ tRNA-optimized First-Strand cDNA Synthesis Kit is designed. These kits allow reverse transcription at high temperatures and without RNase H activity, ensuring efficient reverse transcription of targets with strong secondary structures and providing increased specificity, higher yields of cDNA. In addition, the RNA Spike-in can be used for monitoring cDNA synthesis efficiency and as a reference for qPCR data comparison.

• Satisfy different needs of customers—suitable for total RNAs, miRNAs, tRNAs and other small RNAs.