[Histonet] gfp-mice

From:

"tamer altay"

Hi,
I have Tie2-GFP labeled mice. I try to image the cerebral vasculature
through a glass cranial window mounted on the surface of the brain
after I remove the bone. A week after the window installment, I wanted
to image the animal under a fluorescein microscope, but I could not
see anything. The vessels were supposed to be emitting the green
fluorescein light.
I am wondering if the light emitted from GFP source, under a glass
cranial window where the vessels are exposed to daylight all the time,
bleaches despite the continuos GFP production.
I would appreciate any information or idea you guys come up with to
help me solve this issue. Thanks a lot!!
Tamer
_________________________________________________________________
Her tür saldiri ve virüslerden korunmanin en güvenli sekli - MSN PC
Koruma'da! [1]Burayi tiklayin!
References
1. http://g.msn.com/8HMAENTR/2746??PS=47575
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