Shield1 Ligand

ProteoTuner inducible protein stabilization systems utilize ligand-dependent destabilization domains (DD) and the Shield1 ligand to reversibly stabilize and destabilize a DD-tagged protein of interest in a predictable and dose-dependent manner. The destabilization domain of the ProteoTuner Shield System was modified from the DD of FKBP12 (1).

Shield1 Ligand

Shield1 stabilizes proteins tagged with a mutated FKBP destabilization domain (DD). It is used to protect DD-tagged proteins from proteasomal degradation. The amount of Shield1 can be varied to tune the amount of your stabilized protein of interest within the cell.

Features

Binds specifically to the DD degron, to inactivate protein degradation

No toxic effect on cells

Can be used in cells and in animal models

Applications

Rapid stabilization of proteins containing the DD tag

In vitro protein function studies

In vivo protein function studies

References

Banaszynski, L.A. et al. (2006) Cell126(5):995–1004.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.