The microplate's key advantage over other plates for high-resolution microscopy is the extremely short distance between the well bottom and the microplate skirt (internal well to skirt bottom = 0.7 mm; external well to skirt bottom = 0.51 mm), rendering ease of high magnification microscopy at perimeter wells without complex microscopic adjustments.

The circular wells allow microscopic work without disruptive shadow using the smallest possible meniscus lens.

The perimeter ditch can be filled with water or medium to reduce edge effect due to evaporation, especially for long incubation periods.