Methods and procedures used not including center-specific data entry methods.

1. Sacrifice the mouse

2. Weigh and record total body weight

3. Place mouse on its back and pin the mouse onto board with extended extremities (inner side of hands and foot)

4. Wipe or wet the mouse with 70% ethanol to control hair and dander

5. Option A (for mice that are undergoing complete necropsy):

Proceed with a complete necropsy and tissue collection according to Centre-specific technical SOP including removal of the heart by dissecting the aortic root immediately above the aortic valves and the superior vena cava above the atria