Immunogen: The VHH-12B was generated by immunizing a dromedary with poliovirus type I Sabin strain, preparing cDNA libraries from lymphocytes, and then selecting for tight poliovirus binders by phage display.

Specificity: VHH-12B binds to a site on the top surface of the expanded poliovirion capsid protein VP3. VHH-12B does not bind to the native, unexpanded virus.

Application Notes: ELISA was used to show the reactivity of VHH-12B for poliovirus (Thys et al, 2010). The interaction of VHH-12B with capsid protein VP3 has been captured using cryo electron microscopy (Strauss et al, 2017).

Antibody first published in:
Thys et al, 2010. In vitro antiviral activity of single domain antibody fragments against poliovirus Antiviral Res. 2010 Aug;87(2):257-64 PMID:20566349Note on publication:
Describes the generation of VHH-12B from a phage display library and its characterisation using ELISA.