Technical Support

Starting with the PathScan® Sandwich ELISA kits listed below, the detection antibody and HRP-conjugated secondary antibody in each of these kits will be packaged in a lyophilized format rather than a traditional liquid format. This format enhancement was driven by a request to increase the shelf life of these kits from 6 months to 1 year from the reference date listed on each kit. An extended shelf life provides superior signal retention over time and maximum flexibility in experimental design. The longer shelf life also streamlines production and helps ensure that your product will be available when you need it.

Matched High Performance Between Kits Containing Lyophilized or Traditional Antibodies

Figure 1:PathScan® Phospho-NF-κB p65 (Ser536) Sandwich ELISA Kit #7173 was tested using traditional kit antibodies (blue bars) or kit antibodies that were lyophilized and reconstituted (red bars). Lysate prepared from Jurkat cells treated with calyculin A was used as a positive control (A), and signal was measured across a range of sample titration points. A lysate prepared from untreated Jurkat cells was tested as a negative control (B).

Figure 2:PathScan® Phospho-mTOR (Ser2448) Sandwich ELISA Kit #7976 was tested using traditional kit antibodies (blue bars) or kit antibodies that were lyophilized and reconstituted (red bars). Lysate prepared from Jurkat cells treated with calyculin A was used as a positive control, and signal was measured across a range of sample titration points.

Figure 3:PathScan® Phospho-TrkA (Tyr490) Sandwich ELISA Kit #7210 was tested using traditional kit antibodies (blue bars) or kit antibodies that were lyophilized and reconstituted (red bars). Lysate prepared from 3T3/TrkA cells treated with NGF was used as a positive control, and signal was measured across a range of sample titration points.

Figure 4:PathScan® Total p21 Waf1/Cip1 Sandwich ELISA Kit #7167 was tested using traditional kit antibodies (blue bars) or kit antibodies that were lyophilized and reconstituted (red bars). Lysate prepared from untreated 293 cells was used as a positive control, and signal was measured across a range of sample titration points.