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Dear Madams and Sirs,
in the protocol you say that you sonicate 1 µg of genomic DNA and that you therefrom use 800 ng for MeDIP but I can' t find an information about the enrichment in percent.I would be obliged if you could give me this information because I want to try MeDIP with low amounts of DNA and so I' m very interested in your protocol.
Thank very much for your efforts!!!
best regardsSabrina Pichler

Hi Sabrina,We do not quantitate the enrichment of methylated DNA recovered after MeDIP in terms of a percentage. We just check for the presence of known methylated sequences and the absence of non-methylated sequences in our MeDIP product. It is possible to roughly estimate MeDIP yield using agarose gel electrophoresis and a DNA stain that will visualize single stranded DNA, such as SYBR Gold.

Hello!
I am trying watch the video on meDIP experiment but only sound is available from the title as DNA preparation and immunoprecipitation. Would you please let me know how I can watch the video perfectly?