Datasheet Extract

Add. information

Add 1 part CD3 to 4 parts deionized water and mix for 5-15 minutes.Add the antibody-HRP conjugate directly into the conjugate diluent and mix for 5-15 minutes. Pipette conjugate onto the plate, or store protected from light at 4C for use at a later date.

Application

ELISA, Add 1 part CD3 to 4 parts deionized water and mix for 5-15 minutes. Add the antibody-HRP conjugate directly into the conjugate diluent and mix for 5-15 minutes. Pipette conjugate onto the plate, or store protected from light at 4C for use at a later date., ELISA ,

Background

Poly-Poly Conjugate Diluent (CD3) is a unique formulation specifically designed for use in polyclonal capture and polyclonal-HRP sandwich ELISA formats. Proprietary additives were specifically selected to provide optimal assay performance when using rabbit or goat polyclonal antibodies in a sandwich ELISA format. Poly-Poly Conjugate Diluent contains a uniquely processed form of rabbit and goat IgG to suppress nonspecific binding of the polyclonal-HRP conjugates to the plate surface. To reduce nonspecific binding interactions, a detergent is also included. Antimicrobial agents have been added to retard bacterial growth. When stored at 2