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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is
committed to providing our customers around the world with the highest quality products.
In addition to direct selling in more than 20 countries in North America, Europe and
Asia/Pacific, Miltenyi Biotec also provides support for our customers through an
extensive distributor network covering dozens of additional countries.

As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is
committed to providing our customers around the world with the highest quality products.
In addition to direct selling in more than 20 countries in North America, Europe and
Asia/Pacific, Miltenyi Biotec also provides support for our customers through an
extensive distributor network covering dozens of additional countries.

Get to know the autoMACS® Pro Highlights

The autoMACS® Pro Separator is a fully automated benchtop instrument for magnetic cell separation of multiple samples. It is the most convenient way to pure cell populations.

Explore the autoMACS Pro Highlights:

The autoMACS Pro Separator is compatible with hundreds of MACS Cell Separation Reagents for easy and fast isolation of virtually any cell type from any species. You can isolate cells from a very broad range of sources, such as:

Peripheral blood mononuclear cells (PBMCs

Whole blood or bone marrow

Single-cell suspensions e.g. from dissociated spleens or lymph nodes

Other dissociated tissues e.g. tumors

Video

Cell separation from whole blood with the autoMACS Pro Separator.

With the autoMACS Pro Separator and Whole Blood MicroBeads it is possible to isolate pure cell populations of interest from whole blood or bone marrow samples very easily within 30 minutes.

By providing maximum flexibility in terms of starting materials, the autoMACS Pro can be used for an incredible variety of applications.

For example, the isolation of CD14+ cells straight from whole blood allows for standardized cell separation and safer handling of potentially hazardous samples. Purified CD14+ cells are well-suited for further flow cytometric, molecular or functional analyses, such as proliferation and activation assays.

Video

Sample cooling with MACS Chill Racks on the autoMACS® Pro Separator

The Medina Research Group at the Centre for Experimental Medicine, Queen’s University Belfast uses their unique autoMACS Pro Separator for studies in the area of Vascular Stem Cell Biology. They mainly isolate monocytes, based on the markers CD14 and CD31. After cell isolation, cells are used for cell culture and functional assays.

Why do they love using the autoMACS Pro? Because it is highly effective in terms of yield and purity for monocyte isolation from blood.

The autolabeling feature ensures reproducibility and consistent results - every time and independent of the user. Walk-away sample processing not only saves hands-on time, but minimizes contact with potentially infectious or dangerous sample material. Thanks to the autolabeling feature, automated cell separation could not be easier!

Video

Walkaway multisampling on the autoMACS Separator

The MiniSampler on the autoMACS Pro Separator allows up to 6 samples to be processed in a single batch - from rare cell isolation to isolation of abundant cells.

The research group from Prof. Bachmann’s lab at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) isolates T cells from PBMCs, using a depletion strategy on their unique graffiti autoMACS Pro Separator. They separate the whole population of CD3+ cells, as well as CD4+ and CD8+ subpopulations. The cells are then either cultured, expanded and genetically modified for UniCAR generation, or used for tumor-specific activation via bispecific antibodies.

Why do they love using the autoMACS Pro? Easy handling with step by step instructions, fast and automated cell isolation, and excellent purities of separated fractions (> 95%).

Positive selection, depletion, or untouched isolation – one column for all your needs

The reusable autoMACS Columns are specifically designed for use with the autoMACS Pro Separator and deliver high cell purities and recoveries. Independent of your starting material, your separation strategy, and the separation program you choose, the autoMACS Column is the perfect fit for any automated isolation performed on the autoMACS Pro Separator!

MACS Cell Separation – Select the best – Teaser

The Experimental Hematology research group at the Clinic and Policlinic for Pediatrics, University Medical Center Carl Gustav Carus in Dresden uses their autoMACS Pro Separator for the isolation of human and murine HSCs, T cells, and dendritic cells. They perform both positive and negative selection strategies with important markers such as BDCA2, BDCA4, CD4, CD8, CD11b, CD105 and a lineage-panel. After cell separation, cells are used for in vivo (e.g. transplantation) and in vitro (e.g. co-culture, stimulation, and analysis) experiments. Why do they love using the autoMACS Pro? Because it is highly time-saving, while providing a fast, reproducible, and cell friendly cell separation. With small modifications, they even obtain better purities using the autoMACS Pro Separator compared to manual separation.

The Schmitz laboratory at the Institute for Immunology, University Dresden uses the autoMACS Pro Separator for the purification of human immune cells, including slanDCs, pDCs, CD1c+DCs, CD4+ T cells, CD8+ T cells, Pan T cells, NK cells, and CD14+ monocytes. Their downstream application is cell culture, either mono cell culture or co-culture with distinct immune or tumor cells. Thereby, a variety of proliferation assays, examination of cytokine expression, and cytotoxicity analysis is conducted.

Why do they love using the autoMACS Pro? The main advantages for this research group are the quick isolation of target cell populations with high purity as well as the easy and self-explanatory handling of the instrument.