To PCR folks:
I've been following the discusions on the "good, the bad and the ugly"
of various thermal stable polymerases used in PCR and other applications.
It seems that a compilation of all the characteristics, tricks and lessons
learned about these polymerases would be of wide intrest to many folks.
I personally have used Taq, pfu and Hot tube polymerases, but wish I could find
more information the details of what they can and cannot do, and special
conditions used for certain applications.
If no one else out there wishes to start gathering this information....for a FAQ on
polymerases.....then...OK...I will do it if you send your information to
me.
Jim
Lovelace Institutes
Jim at audrey.lmf.org