The spider subfamily Schismatothelinae from Colombia is revised. One new species of Euthycaelus Simon 1889 and three new species of Schismatothele Karsch 1879 are named, as well as new geographical records provided. E. guane sp. nov. from Santander, S. hacaritama sp. nov. from Norte de Santander, S. olsoni sp. nov. from Norte de Santander and Táchira (Venezuela), and S. weinmanni sp. nov. from Cundinamarca are herein described, diagnosed and illustrated. Schismatothele is newly recorded from Colombia. Morphological aspects and diversity of the Ischnocolinae and Schismatothelinae from Colombia are discussed. An identification key to species of these subfamilies from Colombia is provided.

A new species of Proshapalopus Mello-Leit~ao, 1923, distributed in the Southwest
Colombia in the Choco Region, is described. A morphological cladistic analysis
based on the previous matrix of a Theraphosinae group confirmed the inclusion of
this species within Proshapalopus. The new species, Proshapalopus marimbai n.
sp., can be distinguished from other species of the genus mainly by the reduced
number of labial cuspules, around 40; in contrast the other species having over 100.
Males can be distinguished by the thickened femur III, presence of intermediate urticating
setae type I-III only, palpal bulb with a considerably long prolateral inferior
keel (from ventral median depression to embolus apex) and the accessory keel pronounced
distally. Females can be distinguished for lacking urticating setae type III
and the non-incrassated tibia IV. This discovery constitutes the fourth species
described for Proshapalopus and the first record for Colombia, expanding the geographical
distribution of the genus, until now known for Brazil only. Other aspects
of distribution and the phylogenetic relationship of the new species are also discussed
here.

The genus Holothele Karsch, 1879 has a confusing taxonomic history, mainly due to a imprecise and
outdated generic diagnosis, perpetuated since its original description. In this work, we propose a new
diagnosis for the genus, redescribe the type species Holothele recta Karsch, 1879 and propose a few
taxonomic changes. Holothele longipes is here considered as a senior synonym of Holothele recta Karsch,
1879, Stichoplastus sanguiniceps F. O. P.-Cambridge, 1898, Dryptopelmides rondoni Lucas & Bücherl,
1972, Dryptopelmides ludwigi Strand, 1907. We also provide updated geographic distribution records
for Holothele longipes and propose the revalidation of Scopelobates Simon, 1903.