Expression and clinical significance of matrix metalloproteinase-17 and -25 in gastric cancer.

Wang Y, Yu SJ, Li YX, Luo HS - Oncol Lett (2014)

Bottom Line:
The expression of MMP17 in the normal gastric and atrophic gastritis tissues was significantly lower than that in the gastric cancer tissues (P<0.05).The expression of MMP17 and MMP25 was significantly associated with the depth of tumor invasion, lymph node metastasis and serous membrane involvement (P<0.05), but not with patient age and gender, or lesion length, site and histological grade (P>0.05).Therefore, this indicates that the expression of MMP17 and MMP25 is increased with the degree of progress of gastric carcinoma.

The aim of the present study was to investigate the expression and clinicopathological features of matrix metalloproteinase 17 (MMP17; also known as MT4-MMP) and MMP25 (also known as MT6-MMP) in gastric cancer. Immunohistochemistry and reverse transcription-quantitative polymerase chain reaction were used to detect the expression of MMP17 and MMP25 in 42 cases of gastric carcinoma and normal tissues, and 40 cases of atrophic gastritis. The expression of MMP17 in the normal gastric and atrophic gastritis tissues was significantly lower than that in the gastric cancer tissues (P<0.05). The expression of MMP25 in the gastric cancer and atrophic gastritis tissues was markedly higher compared with the normal gastric tissues (P<0.05). The expression of MMP17 and MMP25 was significantly associated with the depth of tumor invasion, lymph node metastasis and serous membrane involvement (P<0.05), but not with patient age and gender, or lesion length, site and histological grade (P>0.05). Therefore, this indicates that the expression of MMP17 and MMP25 is increased with the degree of progress of gastric carcinoma. The detection of MMP17 and MMP25 expression may have clinical value in predicting the prognosis of patients with gastric cancer.

Mentions:
The expression of MMP17 protein in the cytoplasm was identified by pale-yellow, brown or tan staining. The MMP17-positive cells had a scattered or nest-like distribution in the gastric cancer tissues, and were markedly expressed on the edge of the cancer nest (Fig. 1A). In addition to the cancer cells, MMP17-positive staining was also observed in nearby cancer stromal cells, which suggested that stromal cells have an important role in the process of tumor invasion and metastasis. The expression of MMP17 in atrophic gastritis and normal gastric tissues is presented in Fig. 1B and C. No significant difference was identified between the expression of MMP17 in the normal tissue and atrophic gastritis specimens (3/42 and 4/40 cases, respectively; χ2=0.21; P>0.05). However, the expression of MMP17 in the gastric cancer specimens was significantly higher than that in the normal and atrophic gastritis tissues (31/42 cases; χ12=38.74; χ22=34.10; P<0.05). No significant difference was identified between the mRNA expression of MMP17 in the normal gastric and atrophic gastritis tissues (0.754±0.074 and 1.226±0.082, respectively; t=0.602; P>0.05), however, an evident difference was observed in the gastric cancer tissues (12.126±0.743; t1 8.079; t2=4.493; all P<0.05) (Fig. 2).

Mentions:
The expression of MMP17 protein in the cytoplasm was identified by pale-yellow, brown or tan staining. The MMP17-positive cells had a scattered or nest-like distribution in the gastric cancer tissues, and were markedly expressed on the edge of the cancer nest (Fig. 1A). In addition to the cancer cells, MMP17-positive staining was also observed in nearby cancer stromal cells, which suggested that stromal cells have an important role in the process of tumor invasion and metastasis. The expression of MMP17 in atrophic gastritis and normal gastric tissues is presented in Fig. 1B and C. No significant difference was identified between the expression of MMP17 in the normal tissue and atrophic gastritis specimens (3/42 and 4/40 cases, respectively; χ2=0.21; P>0.05). However, the expression of MMP17 in the gastric cancer specimens was significantly higher than that in the normal and atrophic gastritis tissues (31/42 cases; χ12=38.74; χ22=34.10; P<0.05). No significant difference was identified between the mRNA expression of MMP17 in the normal gastric and atrophic gastritis tissues (0.754±0.074 and 1.226±0.082, respectively; t=0.602; P>0.05), however, an evident difference was observed in the gastric cancer tissues (12.126±0.743; t1 8.079; t2=4.493; all P<0.05) (Fig. 2).

Bottom Line:
The expression of MMP17 in the normal gastric and atrophic gastritis tissues was significantly lower than that in the gastric cancer tissues (P<0.05).The expression of MMP17 and MMP25 was significantly associated with the depth of tumor invasion, lymph node metastasis and serous membrane involvement (P<0.05), but not with patient age and gender, or lesion length, site and histological grade (P>0.05).Therefore, this indicates that the expression of MMP17 and MMP25 is increased with the degree of progress of gastric carcinoma.

The aim of the present study was to investigate the expression and clinicopathological features of matrix metalloproteinase 17 (MMP17; also known as MT4-MMP) and MMP25 (also known as MT6-MMP) in gastric cancer. Immunohistochemistry and reverse transcription-quantitative polymerase chain reaction were used to detect the expression of MMP17 and MMP25 in 42 cases of gastric carcinoma and normal tissues, and 40 cases of atrophic gastritis. The expression of MMP17 in the normal gastric and atrophic gastritis tissues was significantly lower than that in the gastric cancer tissues (P<0.05). The expression of MMP25 in the gastric cancer and atrophic gastritis tissues was markedly higher compared with the normal gastric tissues (P<0.05). The expression of MMP17 and MMP25 was significantly associated with the depth of tumor invasion, lymph node metastasis and serous membrane involvement (P<0.05), but not with patient age and gender, or lesion length, site and histological grade (P>0.05). Therefore, this indicates that the expression of MMP17 and MMP25 is increased with the degree of progress of gastric carcinoma. The detection of MMP17 and MMP25 expression may have clinical value in predicting the prognosis of patients with gastric cancer.