The regulation of body weight depends upon the calorie intake and expenditure. It is a very complex and highly regulated process. It involves multiples neural circuits with specific neuropeptides, neurotransmitter transporters and receptors and influenced by peripheral signals. The product of obese gene (Leptin) may influence many of these processes. White and brown adipose tissues (WAT and BAT, respectively) play a central role in body weight and energy expenditure. WAT is the major site for energy storage via triglyceride synthesis and mobilization via lipolysis. Uncoupling proteins (UCP1-4) are a family of mitochondria transprt proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. BAT is able to dissipate energy as heat via uncoupled mitochondrial respiration by a mitochondrial anion carrier, uncoupling protein 1 (UCP1). UCP1 is predicted to contain 6 transmembrane (TM) domains, a putative purine nucleotide-binding domain (PNBD) and three mitochondrial energy transfer protein domains (ETPDs). Both N- and C-termini are predicted to be cytoplasmic. UCP5 may be alternatively spliced to produced at least 3 transcripts: UCP5L has a unique 22aa N-terminal sequence; UCP5S is missing 3aa (23-25aa) and UCP5SI has 31aa insertion between TM II-IV.

Catalog #

U1625-03

Applications

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution

Western Blot: 1:1000-1:5000 (ECL).

ELISA: 1:10,000-1:100,000. Coat with 50-100ng control peptide/well.

Optimal dilutions to be determined by the researcher.

Control Peptide

U1625-14: Uncoupling Protein 5, Rat, Control Peptide (UCP5, BMCP1)

Storage and Stability

May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Clone Type

Polyclonal

Isotype

IgG

Host

Rabbit

Source

Rat

Concentration

As reported

Form

Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

Purity

Serum

Immunogen

Synthetic peptide 16aa sequence within the N-terminus of the first extracellualr region of rat UCP5 (KLH coupled).

Specificity

Recognizes Uncoupling Protein 5. The sequence is conserved in all 3 isoforms in rat and mouse UCP5. No significant sequence homology with UCP1-4. Species crossreactivity

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.