Technical Data Sheets

Modified Steiner Using Chapman's Modification

Fixation

Section

Staining Procedure

Sensitize sections in Zinc
Formalin at 60°C in a water bath for
15 minutes (cover jar loosely). Preheat this solution.

Rinse in distilled water, 3 times for 2 minutes each.

Place in Silver Nitrate, 1%, in a Coplin
Jar. Place in microwave (800
watts); start and run for 80 seconds. Remove from microwave and allow
to stand in hot silver solution for 20 minutes (cover jar loosely).

Rinse in distilled water 3 times for 2 minutes each.

Dehydrate in 2 changes of 95% ethanol followed by 2 changes of 100%
ethanol.

Treat with Gum Mastic, 2.5%, for 5 minutes.

Allow to air dry for 1 minute.

Rinse in distilled water (2 changes). Slides may stand in distilled
water while reducing solution is being made, or longer if necessary.

Reducing solution at 45°C
in water bath for 5-30 minutes or until sections have developed satisfactorily
with the spirochetes at desired intensity and the background light yellow.
Avoid an intensely stained background by checking microscopically. DO
NOT PREHEAT THIS SOLUTION.

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