DELFIA Eu-N1 Streptavidin

Overview

DELFIA® Eu-labeled streptavidin is intended for use as a generic reagent to detect biotin-labeled compounds, e.g. antibodies or antigens in various types of immunoassay or nucleic acid probes in hybridization assays based on solid-phase separation. The label, Eu3+, is measured using dissociation-enhanced time-resolved fluorometry.

The streptavidin is isolated from fermentation filtrates of Streptomyces avidinii.1 mg of streptavidin binds aproximately 10 µg of biotin. The preparation is supplied ready to use in 50 nmol/L Tris-HCl buffered saline solution. For DELFIA immunoassays, a final concentration of 100 ng/mL is recommended for the generation of a suitable signal.