CMM media difficulties

posted 21 Sep 2014, 19:55 by Darren Creek

There have been some cases where CMM does not support adequate growth of bloodstream-form T. brucei, and this is most likely due to the FCS. We used FCS Gold (PAA) previously because it is the most consistent between batches (although can still differ by country-of-origin). If you cannot access FCS Gold there are a few tricks that you can try to supplement the media whilst still providing a more biologically relevant medium than HMI9.