Description:
Susceptibility to tuberculosis (TB) in mice has recently been attributed to the IPR1 gene (1). IPR1 is a member of the SP100/SP140 family of nuclear body proteins and encodes a leukocyte-specific nuclear body component. The protein can function as an activator of gene transcription and may serve as a nuclear hormone receptor coactivator (1,2). Alternative splicing has been observed for this gene and three transcript variants, encoding distinct isoforms, have been identified. SP110 is the closest homolog of the IPR1 protein in humans (2). The IPR1/Sp110 gene product might play a role in integrating signals generated by intracellular pathogens with mechanisms controlling innate immunity, cell death, and pathogenesis (1). IPR1/Sp110 is up-regulated after infection with M. tuberculosis and required by Anaplasma phagocytophilum for infection of human promyelocytic cells. Defects in Sp110 are a cause of severely impaired resistance to infection by M. tuberculosis (3,4)

Applications: Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:Western Blot: 1–2ug/mlOptimal dilutions to be determined by the researcher.

Storage and Stability:May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type

Isotype

Clone

Grade

Pab

IgG

Affinity Purified

Size

Storage

Shipping

Source

Host

100ug

-20°C

Blue Ice

Human

Rabbit

Concentration:

As reported

Immunogen:

Synthetic peptide corresponding to 15aa from near the carboxy terminus of the human IPR1 (GenBank accession no. NP_004501).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

Specificity:

Recognizes human IPR1. Species crossreactivity: Mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.