Summary: Real-Time PCR primers sets may be designed using standard primer design algorithms without any modification. As with all PCR amplifications, however, the specific reaction conditions for each set must be optimized, particularly primer concentration, annealing temperature and magnesium chloride concentration.

Protocols

Primer Bank
(Dr. Xiaowei Wang at the Department of Molecular Biology, Massachusetts General Hospital)PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time PCR). There are several ways to search for primers: by GenBank Accession, NCBI protein accession, LocusLink ID, PrimerBank ID or Keyword (gene description). PrimerBank contains about 180,000 primers covering most known human and mouse genes.http://pga.mgh.harvard.edu/primerbank/index.htmlAdded: Fri May 14 2004, Hits: 137, Reviews: 0Write reviewCached