In <6unrddFg2336U1 from mid.individual.net>,
Christian Praetorius <prae from gmx.net> wrote:
> Aawara Chowdhury <aawara from pontiff-playground.org> wrote:
>>>Oddly enough this has failed for me - many, many, times. We use 1 mm gap
>>cuvettes, and 1800 volts (25 uF capacitance, and I think 200 ohms
>>resistance). What are your settings?
>> I used the same settings, when we did electroporation. As long as the
> cells were washed good enough, we never had problems with short
> circuits. Since we have a quite good protocol for making
> supercompetent cells, we moved away from it.
Can you share this protocol with us?
Thanks!
AC
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