Description:
Borrelia burgdorferi is a gram-negative spirochete that is a causative agent of lyme disease in humans.

Applications: Suitable for use in FLISA, Immunohistochemistry and Immunoflourscence. Other applications have not been tested.

Recommended Dilution:FLISA: 1:100- 1:1000Immunohistochemistry: 1:100-1:1000 on frozen section or formal-fixed paraffin-embedded sections after antigen retrieval.Immunoflourscence: 1:100-1:1000Optimal dilutions to be determined by the researcher.

Molar Flourescein / Antibody Protein Ratio: 3-7

Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

Type

Isotype

Clone

Grade

Pab

IgG

Affinity Purified

Size

Storage

Shipping

Source

Host

500ug

-20°C

Blue Ice

Goat

Concentration:

~0.5mg/ml

Immunogen:

Heat-killed whole cells of Borrelia burdorferi.

Purity:

Purified by Immunoaffinity chromatography.

Form

Supplied as a lyophilized powder with BSA. Contains no preservatives. Labeled with FITC. Reconstitute with 1ml sterile ddH2O, 0.09% sodium azide. Additional dilutions can be made using PBS.

Specificity:

Highly specific for Borrelia burdorferi. Cross-reactivity with other Borrelia species has been minimized through extensive affinity adsorption. Has not been tested for crossreactivity to treponemes.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.