You can use this
website to download freeware applications (see links below) written by
G. Mashanov (The Crick Institute, UK). This software was developed for the
detection/tracking, analysis, and modelling of single molecule dynamics (movement and binding kinetics)
in live cells, but it can be used for other purposes. You can download our
real and simulated data samples and ImageJ Plugins to import and export your data
files.

The software was
compiled using CBuilder_XE7. It will run under Win32 or Win64 OS and does not
require installation or registration:

GMimPro is an image sequence processor designed for automatic
single particle/molecule detection and tracking. It can track up to 10000 objects
for up to 10000 frames. You can use ImageJ Plugins to convert your data
files into GMimPro format (“.gmv”) or import RAW data files in GMimPro (File/Import
Data). You can export the results of tracking or other measurements using
“.txt” or “.gmi” data format. See Biophysical Journal, 2007 publication for full description of the employed
algorithms.

GMcellModelis a computer model simulating
mobility and binding kinetics of the single fluorescent molecules (both
cytoplasm and membrane associated) in a virtual cell. Itgenerates a sequence of images
(8-bit “.bmp” or “.gmv” format), each containing summed images of all
fluorescent objects emitting light under given illumination conditions with
realistic levels of noise and emission fluctuations. These sequences can be
analysed by GMimPro or other imaging software (e.g., ImageJ). You
can load few basic scenarios (downloaded folder GMcellModelScenario) and run
the model to test it.See JRS Interface 2014publication for full description of the
employed algorithms.

Motility is a satellite software designed
for statistical analysis of tracking data (.gmi) generated by GMimPro. You can add many
individual “.gmi” files together and create distributions of: intensity,
mobility, velocity, and other paprameters. You can create plots of average
intensity, mobility, and "distance from the origin" versus time, generate MSD
versus dT plots, and others. You can apply thresholds to separate slow-fast,
dim-bright, short-long lived objects, and so on. The graphs can be printed,
saved as “.bmp”, and exported as “.txt” files for future analysis or
publishing.