No significant cross-reactivity or interference between Human T4 and analogues was observed.

Samples

Serum, Plasma, Tissue Homogenate, Feces and Urine

Assay Type

Sandwich

Detection Range

15.6 nmol/L - 500 nmol/L.

Sensitivity

2.0 nmol/L.

Intended Uses

This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated T4 concentrations in Human serum, plasma and other body fluids. Using Purified Human T4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add T4 and T4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of T4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Preparation and Storage

Store all reagents at 2-8 degree C

Product Note

Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Other Notes

Small volumes of T4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for T4 purchase

MBS034919 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Thyroxine (T4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 nmol/L - 500 nmol/L in biological research samples containing T4, with an estimated sensitivity of 2.0 nmol/L. The ELISA analytical biochemical technique of the MBS034919 kit is based on T4 antibody-T4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect T4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, T4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces and Urine. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated T4 concentrations in Human serum, plasma and other body fluids. Using Purified Human T4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add T4 and T4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of T4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.