Hello everyone: I am working on differential display. On the gel, I do see some specific bands. After cut these bands, reamplified by PCR, I did Northern Blot using the PCR rpoducts as probe. However, I have not getten any positive bands in Northern Blot. Does anybody work use differential display? What is the possibility of false positive? Any suggestion. e-mail to yd128 at albanyvms.
Thanks.