Hello all! I'm trying to run a meta-analysis from different RNA-Seq datasets using the package metaRNASeq. I have run the differential expression analysis for the individual datasets using DESeq2 and I obtained the differential expressed genes and the p-values. DESeq2 gives a two-tailed p-value. My question is: should I transform the p-values from two-tailed to one-tailed or I can use them directly as two-tailed to perform the meta-analysis using metaRNASeq package?
Thanks in advance!!