Application Information: MMP22 antibody

WB: Use at an assay dependent dilution. By blotting against the reduced protein, the antibody identifies a band at 53 kDa, as well as larger bands at 110 kDa and 80 kDa. The larger bands may represent alternative splicing, post-translational modifications, aggregation, or other phenomenon. A minimum working antibody dilution of 1/1000 is determined using a tissue cell lysate with an alkaline phosphatase conjugated secondary antibody and BCIP/NBT as the substrate. A starting dilution of 1/5000 of GTX11642 is recommended for chemiluminescent substrates. Optimal dilutions/concentrations should be determined by the end user.

Concentration

1
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Immunogen affinity purified

Purification Note

Affinity isolated antigen specific antibody is obtained from rabbit anti-MMP22 antiserum by immuno-specific purification which removes essentially all rabbit serum proteins, including immunoglobulins, which do not specifically bind to the peptide.

Specifications: MMP22 antibody

Product Description

Rabbit polyclonal to MMP22

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development,reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene lacks the signal sequence found in other MMPs, suggesting that it may function intracellularly. The substrate for the enzyme has not been identified, but it is thought that the protein may play a role in reproductive processes. This protein is encoded by the MMP22 or MMP23B gene which is part of a duplicated region of chromosome 1p36.3, which includes a second gene, MMP23A, which is almost identical.

Synonyms

MMP23B, MMP23A, MMP22, 419407

Cellular Localization

Secreted

Specificity

The antibody specifically binds to MMP22 and does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9, etc.).

Host

Rabbit

Clonality

Polyclonal

Target

MMP22

Isotype

IgG

Immunogen

Synthetic peptide (Chicken) N-term.

Antigen Species

Chicken

Species Reactivity

Chicken

Storage Conditions: MMP22 antibody

Storage Buffer

Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, PBS

Notes

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.