{"files"=>["https://ndownloader.figshare.com/files/934920"], "description"=>"<p>(A) 3D immuno-FISH on B49.5 cells expressing lacI fused to an INM protein, using a DNA probe for lacO and antibody detecting Lamin A. The single image plane shows lacO signals co-localised with foci of lamin A that are at invaginations of the nuclear envelope. Scale bar = 2 µm. (B) Approximate sites of lacO integration (red), determined from interphase FISH with genomic clones (green), on chromosomes 4 and 11 in cell lines B49.5 and J21.C3, respectively. Map position and location of genes is taken from the March 2006 (NCBI Build 36.1) Assembly of the human genome at UCSC (<a href=\"http://genome.ucsc.edu/cgi-bin/hgGateway\" target=\"_blank\">http://genome.ucsc.edu/cgi-bin/hgGateway</a>). Details of clone positions are given in <a href=\"http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1000039#pgen.1000039.s003\" target=\"_blank\">Table S1</a>. (C) Interphase FISH with probes for lacO (red) and neighbouring BAC clones (green) in (top row) lacO tagged cell lines B49.5 and J21.C3 and (bottom row) these same cells lines now expressing lacI-lap2β. Scale bars = 2 µm. To the immediate right of each FISH image the histograms quantify the mean proportion of probe hybridisation signal, normalised to the proportion of DAPI stain (y axis), across the 5 concentric shells eroded from the periphery (shell 1) through to the centre (shell 5) of the nucleus (x axis), for the proximal BAC on the untagged chromosome (open bars) and on the tagged chromosome (black bars) and the lacO sites (red bars) in each of the parental and lacI-lap2β expressing cell lines. n = 35–50 for each cell line. (D) as in (C) but using probes for lacO (red) and chromosome paints (green).</p>", "links"=>[], "tags"=>["localisation", "laco-tagged"], "article_id"=>605358, "categories"=>["Genetics", "Cell Biology", "Molecular Biology"], "users"=>["Lee E. Finlan", "Duncan Sproul", "Inga Thomson", "Shelagh Boyle", "Elizabeth Kerr", "Paul Perry", "Bauke Ylstra", "Jonathan R. Chubb", "Wendy A. Bickmore"], "doi"=>"https://dx.doi.org/10.1371/journal.pgen.1000039.g002", "stats"=>{"downloads"=>4, "page_views"=>51, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Subnuclear_Localisation_of_lacO_Tagged_Chromosomes_/605358", "title"=>"Subnuclear Localisation of lacO-Tagged Chromosomes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2008-03-21 01:29:18"}

{"files"=>["https://ndownloader.figshare.com/files/460681", "https://ndownloader.figshare.com/files/460801", "https://ndownloader.figshare.com/files/460833", "https://ndownloader.figshare.com/files/460874"], "description"=>"<div><p>The spatial organisation of the genome in the nucleus has a role in the regulation of gene expression. In vertebrates, chromosomal regions with low gene-density are located close to the nuclear periphery. Correlations have also been made between the transcriptional state of some genes and their location near the nuclear periphery. However, a crucial issue is whether this level of nuclear organisation directly affects gene function, rather than merely reflecting it. To directly investigate whether proximity to the nuclear periphery can influence gene expression in mammalian cells, here we relocate specific human chromosomes to the nuclear periphery by tethering them to a protein of the inner nuclear membrane. We show that this can reversibly suppress the expression of some endogenous human genes located near the tethering sites, and even genes further away. However, the expression of many other genes is not detectably reduced and we show that location at the nuclear periphery is not incompatible with active transcription. The dampening of gene expression around the nuclear periphery is dependent on the activity of histone deacetylases. Our data show that the radial position within the nucleus can influence the expression of some, but not all, genes. This is compatible with the suggestion that re-localisation of genes relative to the peripheral zone of the nucleus could be used by metazoans to modulate the expression of selected genes during development and differentiation.</p></div>", "links"=>[], "tags"=>["recruitment", "periphery", "genes", "cells"], "article_id"=>150780, "categories"=>["Genetics", "Cell Biology", "Molecular Biology"], "users"=>["Lee E. Finlan", "Duncan Sproul", "Inga Thomson", "Shelagh Boyle", "Elizabeth Kerr", "Paul Perry", "Bauke Ylstra", "Jonathan R. Chubb", "Wendy A. Bickmore"], "doi"=>["https://dx.doi.org/10.1371/journal.pgen.1000039.s001", "https://dx.doi.org/10.1371/journal.pgen.1000039.s002", "https://dx.doi.org/10.1371/journal.pgen.1000039.s003", "https://dx.doi.org/10.1371/journal.pgen.1000039.s004"], "stats"=>{"downloads"=>21, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Recruitment_to_the_Nuclear_Periphery_Can_Alter_Expression_of_Genes_in_Human_Cells/150780", "title"=>"Recruitment to the Nuclear Periphery Can Alter Expression of Genes in Human Cells", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2008-03-21 00:13:00"}