In a direct ELISA against purified rat IgG 10 ug/ml as sample, conjugate diluted 1/125,000 produced an O.D.=1.0 in a 15 minute reaction with TMB as substrate. Optimal conjugate working dilutions should be determined for your particular assay conditions. Goats were immunized with purified rat IgG in Freund's adjuvant. Anti IgG was immunoaffinity purified and conjugated with horseradish peroxidase (HRP)

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Antigen Species

Rat

Species Reactivity

Rat

Application Note

Works in most applications. Optimal working dilutions should be determined experimentally by the end user.

Conjugation

HRP

Form Supplied

Liquid

Purification

Affinity purified with antigen

Concentration

1
mg/ml
(Please refer to the vial label for the specific concentration)

Storage Buffer

PBS, pH 7.2 with 0.2% BSA, 0.1% sodium azide

Storage Instruction

Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.

Notes

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.