Documentation

SDS

Certificates of Analysis

Title

Lot Number

344045

Enter Lot Number

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision

28-September-2007 RFH

Description

A protease inhibitor cocktail specifically developed for the inhibition of protease activity during protein purification and sample processing. Suitable for use with yeast, bacterial, plants, and mammalian tissues. No EDTA is used in this preparation. Compatible with FOCUS-Nuclease™ for removing nucleic acid contamination from samples. FOCUS-Protease-Arrest™ contains agents that inhibit serine, cysteine, metallo-proteases as well as calpains. FOCUS-Protease-Arrest™ contains both irreversible and reversible protease inhibitors. FOCUS-Protease Arrest™, used at 1:50 dilution in extraction buffer (pH 7-8) inhibits over 95-98% of protease activities.

Form

Liquid

Formulation

Supplied as 100X.

Recommended reaction conditions

1. Allow solution to warm to room temperature. The solution is in suspension form, vortex before pipetting.
2. Add FOCUS-Protease Arrest™ Cocktail at a volume of 10 µl/ml of extraction/lysis buffer or extract to achieve a 1X final concentration. Note: For greater protease inhibition, the Protease Arrest™ Cocktail may be added to extraction/lysis buffer at a volume of 13 &microl/ml (1.3X final concentration).

Storage

+2°C to +8°C

Do Not Freeze

Ok to freeze

Special Instructions

Following dilution in extraction/lysis buffer or extract, the FOCUS-Protease Arrest™ inhibitors are stable for up to 2 weeks at 4°C or up to 4 months at -20°C.