The Tetra·His Antibody, BSA-free is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Performance

The sensitivity of Tetra·His Antibody in western blots is up to 2 ng, and up to 0.5 ng in dot blots (see figure "Sensitivity of Anti·His Antibodies"), when carrying out detection using a chromogenic substrate (sensitivity may vary with different proteins).

Proteins detected with QIAexpress Anti∙His Antibodies.

Protein

Tag location

Sequence

A

DHFR

N-terminus

MRGSHHHHHHGSVRPLNCIV..........

B

DHFR

C-terminus

.........VYEKKDGSRSHHHHHH

C

Thioredoxin

N-terminus

MRGSHHHHHHGSSDKIIHLT..........

D

TNF-α

N-terminus

MRHHHHHHQSTESMIRDV.........

E

TFIIAγ

N-terminus

MGSSHHHHHHSSGLVPRGSH.....

F

Chaperonin

C-terminus

..........AKIAKDGSRSHHHHHH

G

DNA polymerase

C-terminus

..........EDWLSAKERSHHHHHH

H

TFIIAαß

Internal

......QQQHQPQQQQHHHHHHHQQAQPQQTVP....

Principle

The QIAexpress System provides materials for the expression, purification, detection, and assay of 6xHis-tagged proteins. For detection, QIAexpress mouse monoclonal antibodies are produced under conditions that guarantee the highest purity and activity. Hybridoma cell culture is carried out under serum-free conditions, ensuring that the preparations are free of viruses, mycoplasma, and contaminating immunoglobulins. Purification by adsorption chromatography is performed entirely at physiological pH, yielding preparations with the highest proportion of native antibody.