Summary

Secondary Antibodies

Negative Isotype Controls

Useful Reagents

Positive Controls

Histology Controls

More Images

References

Reviews

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Rabbit anti PAK2 antibody recognizes PAK2 also known as p21 protein (Cdc42/Rac)-activated kinase 2. PAK2 is a member of the p21 activated kinases (PAK) which act downstream of Rho GTPases. PAKs are involved in regulation of the cytoskeleton and nuclear signaling.

Intended Use

Target Species

Human

Species Cross-Reactivity

Target Species

Cross Reactivity

Mouse

Rat

Expected from Sequence

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Reconstitution

Preparation

Antiserum Preparation

Antiserum to PAK2 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Preservative Stabilisers

0.02% Sodium Azide50% Glycerol

Immunogen

Recombinant human PAK2

Purity

Approx. Protein Concentrations

Reagents In The Kit

Preparing The Antibody

Test Principle

Buffer Solution

Phosphate buffered saline

Storage

Store at -20oC Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name

Yes

No

Min Dilution

Max Dilution

Immunohistology - Paraffin

1/50

1/200

Western Blotting

1/500

1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice

Recommended Protocol

ELISA

Immunohistology

Histology Positive Control Tissue

Immunofluorescence

Western Blotting

Rabbit anti PAK2 antibody detects a band of approximately 65 kDa in Jurkat cell lysate and mouse tissue lysates under reducing conditions