Anthracnose disease of mango causes by Colletotrichum gloeosporioides (Penz.) Penz and Sacc. is one of the most devastating pathogen in mango, which behaves differently to varied host crops in their symptom development, severity and adaptability. So, an in vivo study was conducted to know reaction of some fruits crop and weeds to C. gloeosporioides. Our study found that the pathogen was capable to infect all fruit crops evaluated (citrus, grape, guava, papaya and strawberry) and two weeds (Ulta kanta and Santhi), but failed to infect three weeds (amaranthus, cassia and kanghi butti). Mycelial growth pattern and pigmentation varied from circular to suppressed and white, pinkish to black respectively. This cross inoculation experiments will help us to demonstrated variations in the level of host preference among C. gloeosporioides isolates from different fruit crops and weeds host and help in study of survival of fungal pathogen.