Can be used in research studies to identify Apo(a) in Lp(a) present in human plasma or serum using a capture ELISA or to identify purified Apo(a) (purified by native gel electrophoresis) in Western blot analysis.

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.Shelf life: one year from despatch.

Format

Purification:

Protein A chromatography and 0.22 µm filteration.

Buffer System:

PBS buffer, pH 7.0 with 0.09% Sodium Azide as preservative.

State:

Liquid purified Ig fraction (>95% pure)Purified

Specificity

Specificity:

Binds in ELISA (both indirect and sandwich) to native APO (a) in Lp(a) and shows no cross-reactivity with other apolipoproteins or plasminogen.All isoforms of human Apo(a) are recognized.This specificity was confirmed by Western blot analysis (native agarose gels) using a peroxidase-labeled secondary antibody and diaminobenzidine substrate.