Odyssey® CLx Infrared Imaging System

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The Odyssey® CLx Infrared Imaging System is a direct near-infrared fluorescence detection system.This imager is based upon the LI-COR industry-standard Odyssey Classic system. The Odyssey CLx provides better data with its proven quantitative Western blot capability, while not compromising on the sensitivity researchers have grown accustomed to with film. The Odyssey CLx offers versatility in research through numerous applications, including Western blots, In-Cell Western™ Assays and other plate-based assays, cell-based assays, ELISA, EMSA, DNA gel documentation, Coomassie gel imaging, protein arrays, tissue section and organ imaging, and small animal imaging. All of this, in a platform that provides a lab with a film-free environment and a simple, intuitive interface.Included is Image Stu...Read more

"The LiCor Odyssey CLX is a pleasure to work with. The detection quality is excellent and the software is both easy to use and provides clear quantification. In addition, technical support is top notch."

"Odyssey® CLx Infrared Imaging is a very efficient system for western blot (WB) detection. It works with two different wavelength fluorescent antibodies. This means you can reveal two antibodies (produced in different animals) at the same time. Just incubate them for one hour (they can be reused many times if stored at 4ºC). So, you discard the ECL (enhanced chemiluminescence) and dark chamber for reveal WB. You can adjust the laser intensity and speed of scanning to increase the results. Moreover, you can incubate the membranes and reveal them many days after if necessary, once the fluorophores are more stable. For this you should dissolve them into TBS buffer solution at 4 ºC. This machine can be used for a lot of functions, including In-cell western, On-cell western, animal scanning (need a coupling system), gel scanning, etc. The product has a resistant glass and works with both PVDF or nitrocellulose membrane, however, the last one is better for stronger laser intensity."

"The equipment is versatile and easy to use. It saved my group a lot of money as compared to the traditional western blotting. It is faster than other analysis options of bands with the easy to use software. More so, the technical support is amazing. We had free hands-on training and regular support."

"Odyssey INFRARED scanner imaging system is used by our lab regularly for scanning western blots. It works very well without any issue but sometimes it says program is busy so we have to restart the instrument. Other than this minor disadvantage it is very useful for scanning blots and is very reliable. I would recommend this equipment for any lab."

"It quantifies expression of your protein precisely. However, it is very pricy for the service that you get. I'd recommend waiting a bit for the price to come down before purchasing a $57,000 machine. "

"The Odyssey imaging system allows me to do multi-epitope imaging. Allowing for easy quantification of changes of post-translational modifications. The software interface has improved greatly over the years and is great and easy to use. There are so many things available now that make it easy to get the best image possible every time. If you are not using this and still using ECL and film, you should really switch over to this technology. While it is not as sensitive, the fluorescence vs ECL, you can order specific models that allow you to image using ECL without film. When I start a lab, if I do, I will buy one of these imagers without question."

"Very handy device for multiplexing. Results are very quickly obtained compared to traditional WB (ECL). However, scanning time is not that fast. Some time must be invested to find a good membrane blocking agent. The detection limit, in my opinion, is not as high as in traditional ECL."

"We use the Odyssey primarily for western blots and love our machine! The scan is a bit slow depending on the resolution. Sales support and tech support are very happy to assist with any problems or questions."

"This product is easy to use. Both the machine and software are fairly intuitive, and one can begin using the machine after very little instruction. Labs may need to adjust their regular protocols to accommodate infrared signals, but the initial effort is worth the time."

"This has a precise detection technique and highly sensitive. This works fine with Western Blot with minimum amount of protein transferred to the membrane. I also like the analysis software which is great to work with and easily controllable."

"The sales representative and installation team were great at demonstrating how to work the machine as well as giving other insights on how to better our techniques. The equipment cuts down so much time and produces a great image. I would recommend this equipment to my colleagues."

"The Odyssey is very user friendly. I've used it in multiple applications and have noticed a higher quality output compared to traditional techniques. The availability of LI-COR Odyssey antibodies makes detection of multiple proteins very easy."

"I have enjoyed using the Odyssey-it is easy to use, the software is intuitive, and the quantitation is easier to acquire than typical chemiluminescent methods. Some antibodies do not work well with fluorescence and therefore don't work as well on the Odyssey-but those that do work, work well and the antibodies can typically be re-used at least 3 times (if you adjust the gain). I recommend the Odyssey, but keep some of your chemiluminescent reagents around in case the antibody doesn't work well for fluorescence."

"I use the Odyssey CLx primarily for Westerns. It is cleaner, easier, and faster. It's easy enough to use that I send my undergrads to work with it unsupervised. The LI-COR staff are great and have helped me figure out any problems I have encountered."

"I have been using the Odyssey for two years and love it! I will never go back to X-rays for detection.
Advantages for Western Blotting:
1. Sensitivity : Need less protein loading and antibodies
2. If using monoclonal and polyclonal antibodies, can detect the two in the same blot (I hate stripping blots)
3. Ease of quantitation
4. Less background as we can adjust the scanning intentities
Advantages for In-Cell Western :
1. Detection and quantitation of proteins in primary cells where starting protein is less
2. Less antibody required, so save money
3. Perform experiment in replicates, so statistics are easy after quantitation.
Advantages of On-Cell Western :
1. Can detect and quantitate expression of "extracellular" proteins. This technique gives it an advantage over traditional Western Blotting .
2. Less cumbersome and easier than membrane extraction and western blots. "

"I have been using the Odyssey since 2010. I love it and haven't used ECL since. I was sold on it when I found out there was no more substrate, no more film, no more developer problems, and no more getting jumped in line for the dark room. The improved sensitivity and the flexibility of being able to scan repeatedly, not to mention hours, days, or even weeks later without signal loss is definitely a bonus. There was a little bit of a learning curve, but now I find it easier to quantitate than other methods I have used in the past. Overall, the Odyssey has improved the quality and reliability of my data."

"Have been working with Licor Odyssey infrared imager for more than a year and its great. No more hassles with ECL, running to dark room before the intensity fades or films. The system is sensitive and specific. The availability of two colors makes it possible to probe for two different antibodies with different secondary at the same time, even if the molecular size is the same, for eg. AKT and pAKT. Less stripping so less loss of protein from the blot and more accurate results. It’s also very cost effective as we save on the secondary, ECL and Films. The band intensity can be quantified, saved and transferred right away, to save time. The results are stored on the machine and you can transfer it to your computer as well so there is backup. Over all the machine is great and worth the money. Would highly recommend."

"The machine is amazing! Although it has been out for quite some time, my first experience with the Odyssey was about 2.5 years ago. The ability to visualize more than one protein per membrane cuts valuable time. Although you are limited to using the blocking buffer that is manufactored by Li-COR, the reliabilty and effectiveness of this machine makes the cost well worth it. I also want to praise the technical assistance team. Anytime we have had issues, we call the 1-800 number and the problems are resolved quickly. This equipment is well worth the cost."

"We are a postmortem lab so tissue is very precious. The LI-COR Odyssey allows us to veiw our Western Blots quickly and efficiently. Being able to double label with the 700 and 800 IR laser also allows us to save blots and time. Thank you LICOR Odyssey."

"Very easy to use, even for beginners; very fast when you have a lot of blots to analyze (it can scan as many as you want at the same time) and it's easy to clean and to maintain. Maybe it is not as sensitive when you want to assess phosphorylated proteins."

"Fantastic device. Software is a bit hard at first but great technical support."

Description

The Odyssey® CLx Infrared Imaging System is a direct near-infrared fluorescence detection system.

This imager is based upon the LI-COR industry-standard Odyssey Classic system. The Odyssey CLx provides better data with its proven quantitative Western blot capability, while not compromising on the sensitivity researchers have grown accustomed to with film. The Odyssey CLx offers versatility in research through numerous applications, including Western blots, In-Cell Western™ Assays and other plate-based assays, cell-based assays, ELISA, EMSA, DNA gel documentation, Coomassie gel imaging, protein arrays, tissue section and organ imaging, and small animal imaging. All of this, in a platform that provides a lab with a film-free environment and a simple, intuitive interface.

Included is Image Studio Software, an extremely intuitive, easy-to-use imaging software developed for the Odyssey CLx Infrared Imaging System. It has an intuitive, application-driven ribbon interface that makes training for new users fast and simple. Also includes the time-saving AutoScan feature. Contact LI-COR for more information on various package configurations and pricing. For availability outside the United States, contact your local sales office.

AutoScan Function - Eliminate the guesswork of finding the correct manual intensity and need for re-imaging data.This function results in a dynamic range of greater than six logs, with minimized saturation.

Two-Color Detection and Quantification - Detect two targets simultaneously on the same membrane increases the accuracy of quantification and comparison.

High Sensitivity - Near-infrared fluorescence provides sensitivity equal or better than chemiluminescence giving you low background, high signal-to-noise, multiplexed detection, and the sensitivity of any fluorescent system.

Direct Detection for Better Data and a Cleaner Planet - No film, a darkroom, plastic wrap, or substrates.

IRDye signals on membranes are stable indefinitely if stored properly.