One–day training on the use of rapid diagnostic tests (RDTs) successfully provided adequate skill and competency among health workers to perform RDTs in fever case management at lower level health care facilities (LLHFs) in a Uganda setting. The cost averaged at US$101 per health worker trained, with the main cost drivers being trainee travel and per diems. Given the good peer training noted in this study, there is need to explore the cost–effectiveness of a cascade training model for large scale implementation of RDTs.