For each well of cells, add 0.1-2.50 μl of Lipofectamine® LTX Reagent into the above diluted DNA solution, mix gently and incubate for 30 minutes at room temperature to form DNA-Lipofectamine® LTX complexes.

After the 30 minute incubation, add 100 μl of the DNA-Lipofectamine® LTX complexes directly to each well containing cells and mix gently by rocking the plate back and forth.

Complexes do not have to be removed following transfection. Incubate the cells at 37°C in a CO2 incubator for 18-24 hours post-transfection before assaying for transgene expression.

Notes:

Results are best for NPC’s differentiated for 1 day (compared to 7-day differentiated).