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Hilary Hirtle

MaLisa Spring, State Coordinator for the Ohio Dragonfly Survey, sat down with us to talk about the Ohio Dragonfly Survey and its focus – dragonflies and damselflies!

Hilary: “Tell us about yourself!”

MaLisa: “I completed a Bachelor’s in Biology with a minor in Spanish at Marietta College, Ohio. While I was there I did a couple of research projects related to insects and completed my senior thesis on bee diversity. It was then that I decided that I wanted to go to grad school, and I ended up attending OSU, where I received my Master’s degree in Entomology.

I worked with native pollinators for my undergraduate, but I also did a small project on lady beetle diversity and completed an internship on insect diversity. Overall, I’m just broadly interested in entomology and so when there was a mention of a dragonfly survey coming up and that they were interested in hiring someone for the survey, I was all for it – it sounded great! I then landed the job as the state coordinator for the Ohio Dragonfly Survey in May of 2017.

Hilary: “What is the Ohio Dragonfly Survey and what is its goal?”

MaLisa: “The Ohio Dragonfly Survey is a citizen science project with the goal of getting people outside, to notice dragonflies and damselflies, and to submit their observations to the survey via iNaturalist.org. Our goal right now is to figure out where species are throughout the state and to document the status of the threatened and endangered species. Ohio has 167 species of dragonflies and damselflies and we have 23 species that are state-listed as either endangered, threatened, or a species of concern, but there could be several more species added to that list. Additionally, the Hines Emerald (Somatochlora hineana) is the only federally endangered species in our state and it hasn’t been seen since at least 1989, so it might be extirpated (locally extinct, but surviving elsewhere).”

Double-striped bluet

Hilary: “What are some of the greatest threats to dragonflies?”

MaLisa: “There are many threats to dragonflies and damselflies. Habitat loss and degradation are two of the biggest ones. Compared to the species richness between now and say the 1950s, there’s a huge difference. For example, Ohio used to have the Great Black Swamp, a several county-large swamp up in northwestern Ohio, but it was drained and turned into agricultural fields. Reducing hectares of wetlands into ditches created a vastly different habitat for the dragonflies and damselflies of this region, resulting in a significant change in the number of species that used to live here.

Other challenges are pesticides and especially herbicide run off. Herbicides affect plants and certain species of dragonflies and damselflies lay their eggs inside of specific plants. If those plants aren’t there for them to lay eggs in, then the species cannot survive. They also need plants at the edge of water, so if people are mowing down plants at the edge of ponds or other ideal aquatic habitats, then the dragonflies and damselflies don’t have anywhere to emerge after their larvae stage to spread their wings to dry, and so they die.”

Citrine forktail

American rubyspot on water-willow

Violet dancer

Hilary: “What is the life cycle of a dragonfly or damselfly?”

MaLisa: “Immature dragonflies and damselflies, also referred to as water nymphs (naiads) or larvae, reside in the water or aquatic systems, where they can live anywhere between 3 weeks to several years (it depends on the species). Some species are migratory, so they migrate to and from Ohio, but others overwinter in Ohio as larvae in the water systems and then emerge in either the spring or summer (again, it depends on the species) as adults, where their lifespans can range from two weeks to up to a couple of months.

Hilary: “How can you tell a dragonfly and a damselfly apart?”

MaLisa: “Dragonflies and damselflies are in the order odonata and are divided into two separate groups, Anisoptera and Zygotpera. You can differentiate the two based on the wing positioning for the most part, but not always. Dragonflies tend to hold their wings out like a biplane, whereas damselflies tend to hold their wings behind their back like a sailboat.

Eastern pondhawk, a dragonfly

Violet dancer, a damselfly

Another way to differentiate them is based on their size. Most dragonflies are larger than damselflies. But again, there’s an exception to the rule with the body of some damselflies being longer than that of some dragonflies. If you want to get into the nitty gritty to tell them apart, you can look at their eyes to see how separated they are from each other. All damselflies have their eyes well separated on their head, whereas most dragonflies (excluding the Clubtails) have their eyes at least touching.”

Hilary: “For the public to get involved in the survey, do they need to collect specimens?”

MaLisa: “For the most part, we’ve learned that the public doesn’t want to collect specimens, which is okay as photographing them instead is perfectly acceptable. However, if they are interested in collecting specimens we ask that they look on our webpage and follow our collection protocols.”

Hilary: “Will you be at the Open House for the Museum of Biological Diversity on April 7th?”

MaLisa: “I will definitely be here for the Open House event, if anyone wants to ask me any dragonfly questions!”

Contributing to the survey is as easy as taking photos of dragonflies and damselflies and submitting them to iNaturalist.org! Learn more here.

Want to learn more about dragonflies and damselflies? Check out this page for upcoming talks, presentations, and community events. Or download a guide to dragonflies and damselflies of Ohio from the Ohio Division Wildlife.

About the Author: Hilary Hirtle is the Faculty Affairs Coordinator at the OSU Department of Family Medicine; her interest in natural history brings her to the museum to interview faculty and staff and use her creative writing skills to report about her experiences.

Jacqualyn Halmbacher, Research Associate at The Columbus Zoo and Aquarium Freshwater Mussel Conservation and Research Center, gave us an inside look on her research regarding freshwater mussels for this month’s staff spotlight.

Jacqualyn: “Freshwater mussels are mollusks that are similar to their cousins, clams and oysters. Mussels are bivalves, meaning that they have two shells that are held together by two adductor muscles and they feed by filtering food such as zooplankton, detritus, algae, and bacteria from the water with their gills. Mussels are an ancient species, actually being traced back to the Triassic Period – 250 million years ago! They’re found on every continent except Antarctica and one third of the world’s mussels are found in North America, with about 80 species of mussels in Ohio alone – with more species found in Little Darby Creek than all of Australian and European species combined!”

Giant floater Pyganodon grandis

juvenile wavy-rayed Lampmussel Lampsilis luteola

Hilary: “Can you tell us more about the facility?”

Jacqualyn: “The Columbus Zoo and Aquarium Freshwater Mussel Conservation and Research Center is a research and educational facility that’s dedicated to freshwater mussels and other freshwater organisms. Animals we house in this facility are for research purposes and will be sent back to the river systems that they came from. Some stay here long-term. Also, another large portion of the animals housed in this facility are for host work, which is important in propagation and knowing where the reared mussels should be released.”

27 river tanks housing 40 species of freshwater mussels

Hilary: “How does the life cycle of a mussel work?”

Jacqualyn: “Freshwater mussels have a unique life cycle that includes a larval stage where they parasitize a fish. Once a female mussel’s eggs have been fertilized, they develop into larvae called glochidia, which will attach and develop on the gills of fish once the female mussel releases them into the water. They will remain on the gills of fish for up to two weeks, where they will receive the nutrients necessary to grow and develop as they metamorphose into juveniles. Once the full transformation is complete, they will leave the host to live independent lives. What I’m doing in my current research with in vitro is simulating the conditions that the glochidia experience when they are on a host fish.”

Thousands of larval mussels (Pyganodon grandis) in culture medium

Preparing petri dishes for incubation

Hilary: “Propagation? Can you tell us more about this?”

Jacqualyn: “We propagate species naturally by using host fish, but recently we have been working on propagating them artificially in vitro. Freshwater in vitro is accomplished using cell culture techniques, which is removing cells or tissues from an animal or plant and placing them in an artificial environment for survival. The requirements for mussel survival include an environment with controlled temperature, maintaining a particular pH, osmolality, and a growth medium. The culture medium is generally composed of amino acids, vitamins, glucose, salts, proteins, hormones, growth factors, and antibiotics.”

Host fish housed within the facility

juvenile Pyganodon grandis– day 1 (filtering algae)

larval Lampsilis ovata– day 18 (in media)

Hilary: “Why is this important to study?”

Jacqualyn: “It gives us the ability to produce more juveniles in one dish than what we could get from several fish, while simultaneously allowing us to see mussel growth and development. Overall, studying mussel growth and development allows us to create successful conditions for breeding and conservation efforts for mussels, which in turn also helps us better protect their freshwater environment. 70% of mussel species are endangered and 37 species of mussels are extinct. Such species loss may have cascading effects through entire stream food webs, so the research we’re undertaking is important to protecting entire stream ecosystems.”

Hilary: “What projects are you working on now?”

Jacqualyn: “We have a propagation project going on in Illinois in conjunction with BP to propagate and release federal and state endangered species in the Kankakee River. I’m also trying to improve mussel rearing methods.”

Hilary: “What’s your favorite part about working at the facility?”

Jacqualyn: “The challenges that the research presents. There is constantly a new problem that needs to be solved in order to move forward. And I love the new microscope we have! I’ve been able to focus on details that you would never even realize were there.”

Hilary: “Have you made any recent discoveries?”

Jacqualyn: A personal accomplishment of mine is using in vitro to propagate mussels. I know that other researchers have been successful with it, but one thing that I’ve learned is that reading a research paper about an experiment and actually trying to duplicate it is something completely different. There are just so many components involved and so many things that happen that you just don’t account for until you’re in the middle of it.

If you want to learn more about freshwater mussels in Ohio and how to identify them, consider attending one of the mussel ID workshops regularly held at the Museum of Biological Diversity. Please contact Tom Watters, curator of mollusks, directly.

About the Author: Hilary Hirtle is the Faculty Affairs Coordinator at the OSU Department of Family Medicine; her interest in natural history brings her to the museum to interview faculty and staff and use her creative writing skills to report about her experiences.

We met up with Scott Glassmeyer, a student research assistant in the Fish Division, to get an inside view on his role in the Museum of Biological Diversity.

Hilary: “What is your major?”

Scott: “My major is Forestry, Fisheries, and Wildlife, with a specialization in Fisheries and Aquatic Science. I’d always loved fish since I was a kid and before I got into this program, I didn’t know that you could go to college to study fish, or do anything relevant with fish in a job, besides working to commercially collect fish. So, I did research to see if there were any higher education programs that involved learning about fish and aquatics and I found that Ohio State had this program.”

Hilary: “How long have you been a student research assistant in the Fish Division?”

Scott: “Since spring of 2016, but I started as a volunteer in January of 2016 where my primary role was to take older jars containing fish specimens and place them in new ethanol, to better preserve the fish.”

Fish in ethanol

Hilary: “What is the mission of the Fish Divsion?”

Scott: “To preserve historical records of species of fish for future reference and overall long-term data collection and education. It’s a way to validate that this species of fish was recorded in a particular area and a specific species was recorded in general, as fish get misidentified a lot. So it improves a lot of accuracy regarding records.”

Hilary: “What fish are housed here?”

Scott: “Mostly Ohio fish, but we have some from the entire 50 states as well. There are also some fish species from other countries, some saltwater fish, and some aquarium fish here as well.”

Hilary: “Are the specimens here largely donated?”

Scott: “A lot of the specimens are collected through the museum, as well as the Ohio EPA. The Ohio EPA has a division that monitors streams and stream quality statewide and they will collect fish in the process and send them to us.”

Staff collecting fish with seine nets

Hilary: “How are the fish preserved?”

Scott: “The way the preservation process works is that you put the fish specimens in formaldehyde for a certain amount of time, then you place them in water for about a day, before you start adding the ethanol bit by bit, as you slowly add larger amounts of ethanol to build up the tolerance – and that’s what they stay in. It takes up to a week and a half to two weeks to put them in this preserved state.”

Hilary: “Why is it important to study these fish?”

Scott: “It’s really important to study these fish because it helps you not only understand the water quality of their habitat, but also the intrinsic value of their ecosystems. For example, if you have a stream that’s just concrete because it was filled in, this could possibly only allow for about 5 species of fish to live there, whereas before, when the stream had natural morphological features and geological shapes, there were a lot more species of fish living within in this habitat.”

“A good example of this is from about 6 or 7 years ago, when the 5th Avenue Dam along the Olentangy River near campus was removed. Trees, plants, and wetlands were added along the bank and this natural state contributed to the value of the stream, not just for people, but for the fish as well, as this improved quality increased the level of biodiversity within in and around the river.”

Scott Glassmeyer holding a Giant bottlebrush crayfish

Hilary: “What’s your favorite part about working in the Fish Division?”

Scott: “I love going outside, putting waders on, getting in the stream and finding fish. You can read all you want about how healthy a stream is, but when you go out there and you see the biodiversity in the water as you collect data, you can tell just how healthy the water is and it’s wonderful.”

“I also really like the people who work here with me. Everyone’s very patient here and they take the time to help you out as your learning, which is really nice as learning to identify fish for the first time involves a learning curve.”

Hilary: “What is a project that you’re working on now?”

Scott: “I’ve been editing photographs of fish taken by Brian (my colleague who is the Sampling Coordinator in the Fish Division) and getting them ready to be put into the field guide version of the Fishes of Ohio.”

“The Fishes of Ohio was a guide written in the ‘50s, by Trautman, and then it was revised in the ‘80s by Trautman, and so what we’re working on now would be the next revision. There’s around 190 species or so of fish in Ohio, including invasive species and extinct species, so we’ve been photographing each species listed in the field guide, oftentimes with more than one picture, as you’re taking pictures of what you use to identify them. For example, for some of the sucker species of fish, you have to show the mouth, as that helps with identification. So with these species, there’s some photographs detailing the mouth from underneath, and there’s some side photographs, so that you can see the shape of the head and the mouth from the side for identification.”

Hilary: “Do you photograph the fish in their habitat?”

Scott: “It depends. There was one species of fish where we went out during their spawning season and had the tank set up to photograph them. We caught them, put them in the tank, and took a picture quickly, as they can lose their colors pretty fast. If a fish we find doesn’t have a particular color, we take them, put them in a cooler with an aerator, and take them away from location to photograph them. It’s a time consuming process, with the drive to the specimen’s location, the set-up, hours of wading for fish, and then the tear-down of equipment and the drive back from the site, so taking them away to photograph them can be easier than doing it onsite.

Hilary: “You said that fish lose their colors – what does that mean?”

Scott: “Fish have pigments in their skin, underneath their scales. There’s a lot of colorful fish in Ohio, like darters and minnows, that will have breeding colors and so, during certain times of the year and certain times of the day (or even after they eat) they’ll get a lot of pigment and colors in them. And even if they’re not a colorful fish, their colors can change. For example, you can take a large mouth bass that has some pattern to it and put it into a bucket that’s really light and pull the fish out ten minutes later, and the fish will look really pale. But if you put it in a dark cooler, the fish is going to remain dark and have more color. The stress levels will impact them.”

Hilary: “Do you have a favorite fish species?”

Scott: “This question’s hard. So, my answer changes every month when I discover a new fish, but currently my favorite fish is the Common Dolphin Fish, or the Mahi-mahi. There’s a reason why I like it: So, over 50% of its diet is flying fish, and that’s pretty cool to me. Also, its maximum life span is five years. A marlin or a swordfish can live to be about 27 years of age, and a medium sized Ohio fish species can live to about 15 years. However, the Dolphin Fish lives such a short span of time compared to these fish, yet it grows extremely quickly, as they get up to 36 pounds in 8 months. And it’s really fast too, swimming speeds up to 50 miles an hour.”

Common Dolphin Fish, or Mahi-mahi

Hilary: “With all of your experience and studies, what do you hope to do in the future?”

Scott: “I’d love to work as a fisheries biologist, working for the environment. It’s challenging to get in those types of roles, as they’re very competitive, but I’m going to try.”

About the Author: Hilary Hirtle is the Faculty Affairs Coordinator at the OSU Department of Family Medicine; her interest in natural history brings her to the museum to interview faculty and staff and use her creative writing skills to report about her experiences.

We sat down with Grant Terrell, the Curatorial Assistant for the Tetrapod Collection, to learn more about him and his role within OSU’s Museum of Biological Diversity.

Hilary: “Are you a student at OSU?”
Grant: “Yes, this is my third year at OSU. When I first started my education here, I was an evolution and ecology major in the EEOB department, but I later added a history double major and a paleontology minor.”

Hilary: “What is your job at the Museum of Biological Diversity?”
Grant: “I’m currently the Curatorial Assistant for the Tetrapod Collection.”

Hilary: “How long have you been in this role?”
Grant: “I’ve been in this role for about 6 or 7 months now, so not too long, but I’ve been with the museum for three years. I actually had my first day of work before my first day of class at OSU! I reached out to the museum before I was a student, as I really wanted to work at the museum and it was a deciding factor in whether or not there was a place for me at Ohio State, as I was so fascinated with museum life and and I wanted to be a part of it. So, eventually I was hired as a Research Assistant, doing basic curatorial tasks, then moved onto Curatorial Assistant and am now the acting Collections Manager.”

Hilary: “What is a tetrapod?”
Grant: “The modern way to define it would be a group that contains mammals, amphibians, reptiles, and birds – their last common ancestor and then everything in between. So, dinosaurs are tetrapods, mammoths are tetrapods, and so on. Modern biology uses these types of definitions (they’re called clade-based definitions) for groups so as to avoid the arbitrary things that you can gain or lose in evolution – like legs.”

These are all tetrapods

Hilary: “Why is it important to study tetrapods?”
Grant: “The Earth is a system and it depends on the inner workings of many organismal groups. Tetrapods are only one component of this system, so I believe studying tetrapods is only as important as the study of any of these other groups. Still, it’s rather impossible for one person to comprehensively study all components of this system, and so we are forced to specialize.”

Hilary: “What is your favorite part about working in the Tetrapod Collection?”
Grant: “Being surrounded by diversity at all times of the day. For my spring break, I took a trip to Costa Rica (which is a biodiversity hotspot), but the diversity of animals in the tetrapod collection is at a magnitude much more dense than what I’ve ever experienced before, even in Costa Rica. And it is all right here in the museum – just drawers and drawers of specimens who have unique stories behind them.”

Grant Terrell with museum drawers of American Robins

Museum drawers with American Robins

Variety among American Robin specimens – note the albino in the middle

Hilary: “What is a discovery that you’ve made while working here?”
Grant: “When you start skinning and preparing the specimens, every mammal or bird, even something as common as a house sparrow – every single one is different. Not a single one is the same and when you’re with this specimen for hours preparing it, you start thinking about it’s life – about how it grew up, what it did throughout its day, what it was doing before it died – just with each individual one, you recognize that no one specimen is the same.”

Canada Goose recently prepared by Grant Terrell

Hilary: “You mention ‘skinning’ and ‘preparing’ specimens – can you elaborate on this? Is this something that you do on a day-to-day basis?”
Grant: “Typically, what I create are called study skins, which are different from taxidermy mounds. Taxidermy is more of an art form where you’re trying to depict an animal in a realistic lifelike pose, while study skins are to give you more of a general shape and visual idea of that particular individual, so you can look at things like molt patterns, color variations, differences in measurements – and they’re made to fit into a museum drawer. Preparing a specimen can sometimes feel like performing surgery, as some of the specimens that we receive have injuries, so you have to be careful with the work that you’re doing so as not to further damage the specimen.”

“Usually just about once a week at most I’ll do skinning because it’s so time consuming and once you’ve started on a skinning project, you can’t necessarily stop. But, if a specimen needs prepared, I prioritize by what I have time to do, what we have room for, and what would be valuable for the collection.”

Hilary: “Do you get these specimens as donations?”
Grant: “The majority of what we get is what we call “salvage” – specimens that are road kills or who have been victims of window strikes. In the past, people would kill the animals in the wild to add to the collection – those specimens are known as voucher specimens, which is essentially taking the species for educational purposes.”

Hilary: “What projects are being worked on now?”
Grant: “One of the biggest projects we’re working on now is to rebuild a relationship with the zoo to try to get future deceased specimens from them. The museum used to have a relationship with the zoo many years ago, but as the years went on there was a breakdown in the relations and we haven’t been getting regular specimens from them since the 70’s, so we’re currently working on building that relationship again. A lot of the animals at the zoo are critically endangered, so we want to preserve as many endangered specimens as we can for study, data collection, and genetic analysis for future generations.”

Barbets from the Columbus Zoo

“There’s also a lot of active research going on in the collections, and one of the big things that troubles us is space – we never have enough space and so we are working on acquiring more storage units – and we’re also undertaking projects to optimize space – such as pulling some of the older specimens that don’t necessarily have any data and trying to make room for the ones that do, so that we can add more to the collection.
I am also working on supervising weekly mammal preparations. I myself have only recently been trained in this- bird prep remains my forté – but I recently prepared a Douglas Squirrel and a Yellow-bellied Marmot, the first of its species in the collection. I plan to prepare a porcupine which we have in our freezer and I am expecting this to be quite the undertaking.”

Tree squirrels prepared by student volunteers

Hilary: “Is everything in the collection used for research?”
Grant: “Most of it. There’s also what we call a “teaching collection” – a lot of which is stuff that is older and outside of Ohio and it’s not as valuable for research, so we usually use it for teaching and outreach.”

Hilary: “What is the craziest thing you have found in the collection?”
Grant: “A dried sheep’s stomach inside of a manila envelope. It was just sitting in a random drawer and I was going through the collection one day, trying to catalogue items that hadn’t been catalogued and I opened up the envelope and there’s this sheep’s stomach. It didn’t have any information associated with it, except a tag that said “ovis,” which is the genus that sheep’s are in.”

“Another one is a raccoon specimen that we received, who had died after getting its head caught in a mason jar and the person who had prepared the specimen had left the rim of the jar around the neck of the specimen as a reminder to how the animal had died. I like that one because, to me, it symbolizes what I was saying earlier about every one of these individuals having a story and a life behind it. It reminds you not to take all of these specimens that we have here for granted.”

the inside of a sheep stomach with papillae

preserved four-chambered stomach of a sheep, a member of the ruminant family Bovidae

racoon specimen with head still stuck in glass jar

About the Author: Hilary Hirtle is the Faculty Affairs Coordinator at the OSU Department of Family Medicine; her interest in natural history brings her to the museum to interview faculty and staff and use her creative writing skills to report about her experiences.

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