Synthetic peptide corresponding to c-Jun (phospho T93). Immunogen is in the range of aa 61-110.
Synthesized phospho peptide derived from human c-Jun around the phosphorylation site of Threonine 93.Database link: P05412

Positive control

IHC-P: human breast carcinoma
WB: HeLa cell extract.

Properties

FormLiquid

Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide.
The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Post-translationalmodificationsUbiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.Acetylated at Lys-271 by EP300.

Ab28854, at a dilution of 1/50, staining c-Jun in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Left image: using ab28854.
Right image: using the same antibody preincubated with the synthesized phosphopeptide.Ab28854, at a dilution of 1/50, staining c-Jun in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Left image: using ab28854.
Right image: using the same antibody preincubated with the synthesized phosphopeptide.

References for Anti-c-Jun (phospho T93) antibody (ab28854)

ab28854
has not yet been referenced specifically in any publications.

Publishing research using ab28854? Please let us know so that we can cite the reference in this datasheet.