Real Time qPCR Master Mix—Terra qPCR Direct SYBR Premix

Terra qPCR Direct SYBR Premix is a 2X master mix that lets you perform real-time PCR (qPCR) directly on crude extracts or dirty samples (such as mouse tail and ear biopsies, or leaf cuttings). The premix contains everything you need for qPCR, including Terra PCR Direct Polymerase—a robust, non-Taq polymerase developed for optimal amplification from whole cells, crude cell lysates or tissue extracts, and purified DNA. The enzyme is perfect for amplifying DNA targets up to 2 kb, even when the GC-content is greater than 70%. The premix also contains SYBR Green I dye, as well as a monoclonal antibody that suppresses polymerase activity up to 98°C, allowing automatic hot start. In addition, Terra qPCR Direct SYBR Premix comes packaged with two ROX formulations—ROX Reference Dye LSR and ROX Reference Dye LMP—that allow you to normalize the fluorescence signal between reactions on instruments that are equipped with this option.

Features

Optimized for use with the laser-, lamp-, or LED-based real-time instrument of your choice

Automatic hot start

Perfect for high-throughput screening applications

Applications

Perform direct real-time PCR from crude extracts and dirty samples, without having to purify your template

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.