A Split-plot experiment was conducted in the growth chamber to determine the effect of sewage water deposits, retained at different depths of soil columns, on development of Tylenchulus semipenetrans. Sandy loam soil columns in PVC tubes were treated with a total on I of tap water, 7 or28 I. of sewage water in 14 days. Soils from different depths (0-7, 7-14and 14-21cm) of columns were then emptied in plastic pots. To each pot, a lime seedling was transplanted and then inoculated with 5,000 second-stage juveniles of T. semipenetrans. Effects of the water treatments on nematode population development were not significantly different. However, population development was significantly higher on seedlings grown in soil collected from the lowest depth than from the upper depths of the soil columns.