This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) available for measuring mTFP (Teal) (or other key fluorophores – see complete list above). This state-of-the-art set has been optimized for popular LED based light sources. This set has been designed to deliver significant fluorescence signal improvements for LED based light sources compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps.

Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.

Individual filters and filter sets (no cube):

These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.

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Optical Specifications

Specification

Value

Blocking

BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications

Specification

Value

Microscope Compatibility

BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.

Optical Damage Rating

Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications

Specification

Value

Scratch-Dig

60-40

Exciter/Emitter Orientation

Arrow on ring indicates preferred direction of propagation of light.

Dichroic Orientation

"Reflective coating side" should face toward light source and sample.

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

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