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Tissue Culturing & Other Propagation Techniques of Banana PlantsThis forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.

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Very cool, you should change your name to "TC mad man'
Any of these tc's your doing going to find their way over to the states?

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The first law of thermodynamics, an expression of the principle of conservation of energy, states that energy can be transformed (changed from one form to another), but cannot be created or destroyed.
Source: http://en.wikipedia.org/wiki/Thermod...thermodynamics

today i did tc bananas from male flower and take photo for you all. enjoy it and wish me luck..

step 1. prepare the bananas flower. in this case i have musa velutina (no 1 and 2), new hybrid (3), ensente glucam (4),

Step 2 throw out the skin/layer of flower (what term?) until its only about3-4 cm length and soak in bleach 15 % for 10 minute and then rinse with sterile distilled water 5 times

step 3. open the each layer and take its male flower. best explant is flower in layer no 8 -16 from tip. flower no 1-7 and more than 16 is not good. its callus not embrionic. so cant to turn be shoot.

step 4 put the male flower in media MA. wait 1 month or more until they getting callus. and we can do subculture in same media to get more callus or put in liquid media if do tc cell suspension, or put in shoot induction media.

well, i will post pic later if there is good progress. hah.. i apologize my english is limited to explaining this thread. i hope you all understand what i mean. hah.. tc male flower more complicated than normal tc using the corm. must be carefull. its explant is so little

After 1 month, tc male flower bananas has shows good progress. they forming callus/embrio. bothe ensente and m velutina should remove to new fresh media to get more embrio
Ensente Glaucum. they looks great

Musa Velutina also shows good response, even also producing a little browning

Hi Novi,
Why do you use male flowerparts to TC? Does this give any other results then from meristematic tissue from a sucker?
And do you use other media/hormones to get this to work? Do you have some literature about it?

When you say "its callus not embrionic. so cant to turn be shoot." I think callus doesn't matter right? Can't you induce shootgrowth first, and then root growth from callus?

It is possible to regenerate plants from the callus tissue, but it is not easy or simple. This technique is generally used to establish embryogenic cell suspensions used for somatic embryogenesis. Each somatic embryo develops from a single cell. This is generally only used when genetic engineering the plants so that the transformed plants (with new genetic material) are sure to have originated from a single cell, rather than having chimeras with some transformed and some non-transformed tissue, making it not only unstable, but hard to tell if something has been transformed or not. In Novi's case, I believe it is just for fun to see how far he could get using male flowers. To propagate plants via somatic embryogenesis is more trouble than it is worth for standard micropropagation, so it must just be for the fun of it.

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Growing bananas in Colorado, Hawaii and Washington since 2004.

Are you a banana plant enthusiast? Then we hope you will join the community. You will gain access to post, create threads, private message, upload images, join groups and more.

Bananas.org is owned and operated by fellow banana plant enthusiasts. We strive to offer a non-commercial community to learn and share information. Receive all three issues from Volume 1 of Bananas Magazine with your membership: