Murine reticulocyte lysate. Cathepsin E is expressed abundantly in the stomach, Clara cells and alveolar macrophages of the lung, brain microglia, spleen, and activated B lymphocytes. It is not expressed in resting B lymphocytes.

Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

WB

1/1000. Predicted molecular weight: 42 kDa.

ICC/IF

1/200.

IHC-P

Use at an assay dependent concentration.

Target

FunctionMay have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain.

Tissue specificityExpressed abundantly in the stomach, the Clara cells of the lung and activated B-lymphocytes, and at lower levels in lymph nodes, skin and spleen. Not expressed in resting B-lymphocytes.

Sequence similaritiesBelongs to the peptidase A1 family.

Post-translationalmodificationsGlycosylated. The nature of the carbohydrate chain varies between cell types. In fibroblasts, the proenzyme contains a high mannose-type oligosaccharide, while the mature enzyme contains a complex-type oligosaccharide. In erythrocyte membranes, both the proenzyme and mature enzyme contain a complex-type oligosaccharide.Two forms are produced by autocatalytic cleavage, form I begins at Ile-54, form II begins at Thr-57.

Cellular localizationEndosome. The proenzyme is localized to the endoplasmic reticulum and Golgi apparatus, while the mature enzyme is localized to the endosome.

ab36996 staining Cathepsin E at a dilution of 1/2000 in Human stomach and tonsil tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The sections were PFA-fixed and subjected to Heat-mediated antigen retrieval in TRIS EDTA Buffer pH 9.0 and permeabilization with DAKO wash buffer with Tween. The primary antibody was diluted 1/2000 in DAKO blocking dilution buffer and incubated with the sample for 30 minutes at 20°C. An HRP-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody.