These links provide access to 12 FASTQ files used as part of an RNA-seq workflow of Trapnell et al. This protocol is described on page 524. The FASTQ data are for two conditions (C1 and C2), three replicates (R1, R2, R3), and forward (_1) and reverse (_2) reads. At present, the NCBI website () does not link directly to these FASTQ files.