This antibody gave a positive signal in Mouse embryo brain (E10)tissue lysate, as well as F9 and NIH3T3 whole cell lysates.

性能

形式Liquid

存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

存储溶液pH: 7.40Preservative: 0.02% Sodium azideConstituent: PBSNote: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.

应用

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用

Ab评论

说明

ICC/IF

Use at an assay dependent concentration. PubMed: 24797668

WB

Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 57 kDa).

靶标

功能Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation.

疾病相关Defects in TBX5 are the cause of Holt-Oram syndrome (HOS) [MIM:142900]. HOS is a developmental disorder affecting the heart and upper limbs. It is characterized by thumb anomaly and atrial septal defects.

Thank you for your enquiry. Thus far I have found the following information regarding potential reactivity of our TBX5 antibodies to Scyliorhinus canicula: ab58253 - no chance for cross reactivity ab86370 - region of immunogen overlaps partially but th...