@article {Young958,
author = {Young, JF and Hockmeyer, WT and Gross, M and Ballou, WR and Wirtz, RA and Trosper, JH and Beaudoin, RL and Hollingdale, MR and Miller, LH and Diggs, CL and et, al.},
title = {Expression of Plasmodium falciparum circumsporozoite proteins in Escherichia coli for potential use in a human malaria vaccine},
volume = {228},
number = {4702},
pages = {958--962},
year = {1985},
doi = {10.1126/science.2988125},
publisher = {American Association for the Advancement of Science},
abstract = {The circumsporozoite (CS) protein of the human malaria parasite Plasmodium falciparum may be the most promising target for the development of a malaria vaccine. In this study, proteins composed of 16, 32, or 48 tandem copies of a tetrapeptide repeating sequence found in the CS protein were efficiently expressed in the bacterium Escherichia coli. When injected into mice, these recombinant products resulted in the production of high titers of antibodies that reacted with the authentic CS protein on live sporozoites and blocked sporozoite invasion of human hepatoma cells in vitro. These CS protein derivatives are therefore candidates for a human malaria vaccine.},
issn = {0036-8075},
URL = {http://science.sciencemag.org/content/228/4702/958},
eprint = {http://science.sciencemag.org/content/228/4702/958.full.pdf},
journal = {Science}
}