Hi,
While using a YEp library to clone a gene we identified several multicopy
suppressors. However, we found that one of these genes suppresses when
present on a YCp plasmid.
The original mutant is healthy, so we don't think it is a case where the
multicopy suppressor allows better growth and hence selects for incresed
copy number.
Are there other cases of multicopy suppression from a CEN plasmid?
Thanks
David Stillman
stillman at bioscience.utah.edu