Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

undifferentiated malignant glioma

Age

fetus, 20 days gestation

Applications

The biological characteristics of this tumor closely resemble those of human glioblastoma.

The F98 and RG2 (ATCC CRL-2433) gliomas can be used as rat brain tumor models in experimental neuro-oncology.

This cell line may be used for both in vitro and in vivo studies of a rat brain tumor.

Storage Conditions

liquid nitrogen vapor phase

Derivation

This tumor was produced by Drs. A. Koestner and W. Wechsler at The Ohio State University in 1971.

A pregnant CD Fischer rat on day 20 of gestation was inoculated with a single dose of N-ethyl-N-nitrosourea (ENU) (50 mg/kg b.w.). Tumors were harvested and cloned.

Clinical Data

F98 was submitted to the American Type Culture Collection in June, 1998 at passage 2 from a culture established from a subcutaneous tumor grown in a male Fischer rat.

Tumorigenic

Yes

Effects

Yes, as an intracerebral xenograft in cats.

Yes, in CD Fischer rats

Comments

The transplantable tumor displays an infiltrative pattern of growth within the brain.

It is weakly immunogenic in syngeneic rats and an intracerebral inoculum of as few as 100 cells are lethal.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.