at low passages maintained the diploid karyotype of the normal parental cells but by passage 99 the karyotype had changed to near-triploid.

Derivation

PZ-HPV-7 was derived from epithelial cells cultured from normal tissue from the peripheral zone of the prostate.

Specific amplification of a 160-base pair fragment of the HPV18 E6 transforming region was noted.

Incorporation of HPV18 DNA was confirmed by polymerase chain reaction.

The cells were transformed by transfection with HPV18 DNA.

Clinical Data

70 years adult

Caucasian

male

Tumorigenic

No

Effects

No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.

Comments

Immunocytochemical analysis showed expression of keratins 5 and 8 and also the early region 6 (E6) oncoprotein of HPV.

The cells are negative for prostate specific antigen (PSA).

Incorporation of HPV18 DNA was confirmed by polymerase chain reaction.
Specific amplification of a 160-base pair fragment of the HPV18 E6 transforming region was noted.

Complete Growth Medium

The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF).
To make the complete growth medium, you will need to add the following components to the base medium:

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.