Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

ELISA

WB

ICC/IF

Application notesELISA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
Immunofluorescent and immunoperoxidase staining of infected liver tissues and HBV-transfected hepatoma cells, immunoelectron microscopy.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

RelevanceHepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.

Virtually all anti-HBsAg mabs (including this one) are conformation-specific, i.e., they recognize only native antigen. That's why they do not work usually neither in WB nor in denaturing ELISA.
ab18797 will work in both of those assays, but only the...

I have received feedback from the laboratory saying "Your customer is doing everything right, the dilution is 1 to 1000 and 5% dry milk for 1 hour blocking is what we recommend. At this point we can only assume that something happened to product in tra...

Thank you very much for contacting us.
I just wanted to let you know that I am still working on your enquiry. I have contacted the originator of this antibody for the information you requested, and am awaiting a reply.
I'm very sorry for the del...