What types of CRISPR editing events can ICE analyze?

The current version of ICE software can analyze indels that result from single or multiplex (up to three sgRNA delivered to cells at once) CRISPR-Cas9 double-strand DNA breaks using SpCas9. More features will be added to the ICE software tool soon. To request other nucleases or other features and functionalities, please email support@synthego.com.

The ICE tool also reports a "Knockout score" (KO-score) which represents the proportion of cells that have either a frameshift or 21+ bp indel. This score is a useful measure for those who are interested in understanding how many of the contributing indels are likely to result in a functional KO of the targeted gene.

Future versions of the ICE tool will be able to analyze more complex CRISPR editing, such as knock-ins and single nucleotide variant (SNV) generation. (These are already available in the source code but not yet available for the web frontend).