Intracellular uptake of PcGal16 by HT-1376 and UM-UC-3 bladder cancer cells (panel A). The concentration of PcGal16 was determined by fluorescence spectroscopy and the results were normalized to protein quantity. Data are the mean ± S.D. of at least three independent experiments performed in triplicates. Representative fluorescence images (panel B) of bladder cancer cells incubated with PcGal16 (red) in darkness and cell nucleus stained with DAPI (blue). Scale bars 20 µm.