I am sequencing PCR products directly, using Sequenase, the method is working
very well except for a few cases where parasite sequences mix with the desired
signal. This is apparently due to a double-annealing phenomenon. Any suggestion
as how to avoid this (except to design a new primer!). Thanks in advance.
Jean Mariaux
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Jean Mariaux Phone: (+41) 38 233 051
Institute of Zoology (A115) Fax: (+41) 38 233 001
E.-Argand 11 E-mail: jean.mariaux at zool.unine.ch
CH-2007 NEUCHATEL
Switzerland
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