PT - JOURNAL ARTICLE
AU - Tuite, M. F.
AU - Mundy, C. R.
AU - Cox, B. S.
TI - AGENTS THAT CAUSE A HIGH FREQUENCY OF GENETIC CHANGE FROM [<em>psi</em><sup>+</sup>] TO [<em>psi</em><sup>-</sup>] IN <em>SACCHAROMYCES CEREVISIAE</em>
DP - 1981 Aug 01
TA - Genetics
PG - 691--711
VI - 98
IP - 4
4099 - http://www.genetics.org/content/98/4/691.short
4100 - http://www.genetics.org/content/98/4/691.full
SO - Genetics1981 Aug 01; 98
AB - The [psi] factor of yeast is cytoplasmically inherited. Singh, Helms and Sherman (1979) reported that high concentrations of KCl and of ethylene glycol induce the genetic change from [psi+] to [psi-]. In this study, the following agents have been shown to induce the same genetic change: guanidine hydrochloride at 1 mm, dimethyl sulfoxide at 2.5% v/v and ethanol or methanol at 10% v/v. It is likely that a number of other agents also cause the change, namely 2 m glycerol, m succinate, m glutamate and m MgCl2. Most of these agents induce the change at very high frequencies; with some, the frequency is 100%. Although the observed phenotypic change can also occur as a result of chromosomal gene mutation, no changes of this type were identified. Some of the agents also cause mutation from [rho+] to [rho-] and from killer to sensitive.