Hello,
I've done human exome capture (with Truseq) and sequenced with HiSeq.
I found that the ts/tv ratio is ~1.6 for the dozen exomes that I sequenced.
I am not sure why this is and wonder if this is a bioinformatics issue (i.e.
filtering) or the exome capture issue. I used the same mutation calling pipeline
for my earlier exomes using Agilent SureSelect and there my ts/tv ratio was ~3
thanks
Les