Triglyceride Assay Kit (ab65336) is a sensitive, easy assay to measure triglyceride concentration in mammalian samples. In the triglyceride assay protocol, triglycerides are converted to free fatty acids and glycerol. Glycerol is then oxidized to generate a product which reacts with a probe to generate color (spectrophotometry at λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm).

If your sample contains reducing substances such as glucose, fructose or lactose, they are likely to interfere with the assay. In this case, we recommend using Triglyceride Assay Kit (Fluorometric, Reducing samples) ab178780.

Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

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Some components of this kit are available to purchase as standalone reagents if you need more quantities (lipase, enzyme mix, triglyceride standard or triglyceride probe). Please contact our team for more details.

Triglycerides are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as serving as an energy source. Triglycerides are broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of triglycerides are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.

1) The insoluble top layer may contain the triglyceride you wish to measure so please make sure to solubilize as much as possible (without losing any material) and to follow the recommendations (ie. repeated heating steps). 2) SV is indeed the abbreviation for sample volume. For calculating the final results, you need to take into account all the dilution steps you have made during the procedure so dilutions factors (10 and 60 fold need to be included in the calculation).

Divide by the molecular weight of the substance (average triglyceride molecular weight in your sample) to convert from moles to grams. Then mM/mol weight can be converted to get mg/L.

For this assay, since it is difficult to say the molar mass of triglyceride, unless which specific one is present in the sample is known, the accepted most common way of reporting the data is as mM or µmol/ml.

Mouse blood was obtained by cardiac puncture in heparinized tubes and centrifuged at 13000 rpm for 2 min at 4 degrees. Plasma was subsequently diluted 1:20 in PBS for testing. This kit is easy to use and fast, and gives blood triglyceride concentrations in line with what is reported by literature. Our experience gives reproducible results across the different kit lots.