The CellNetworks nCounter Core Facility has been established to provide access to one of the current state of the art expression profiling technologies for the Heidelberg University research community.

nCounter Core Facility

The CellNetworks nCounter Core Facility has been established to provide access to one of the current state of the art expression profiling technologies for the Heidelberg University research community. The nCounter Analysis System is a complete, fully automated system for the next generation of digital counting of nucleic acids and proteins allowing a 3D biology approach By using a unique coding technology, direct counting of individual molecules is possible. The technology requires only 50 ng of total RNA or equivalent amounts of a raw cell lysate or 300 ng of genomic DNA and 50 ng of protein to study the expression profile, SNPs, methylation sites or copy number of up to 800 targets from one individual sample.

People and techniques involved

The nCounter Core Facility is run by scientific (Prof. Dr. rer. nat. Beate Niesler) and technical staff ( Ralph Röth (Dipl.-Ing.) and Heike Kuzan) and is located at the Institute of Human Genetics (INF 366, 5th floor). The facility houses an Agilent Bioanalyser 2100 and a Qubit for the quality control of samples, an Eppendorf Mastercyler and the nCounter nanostring SPRINT system for DNA, RNA and protein analysis .