Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

grade IV,squamous cell carcinoma

Age

53 years

Gender

male

Ethnicity

Caucasian

Storage Conditions

liquid nitrogen vapor phase

Karyotype

hypotriploid; modal number = 56. The rate of higher ploidies was 21.8%. Cells in this line have complex karyotypes., Over 30 marker chromosomes were found in 5 metaphase karyotypes. The marker chromosomes, t(1q;10q), t(1q;22q), t(2q;3q), der (3)t(2;3)(p11;q29), der (7)t(7;17) (q36;q21) and about 20 others were common to most cells., Among these der (7) was generally paired. Normal N3, N5, N12, N13, N14 and N17 were absent. The X was single and the Y was not found on fluorescence observation.

Derivation

The SW 900 cell line was initiated by A. Leibovitz in January 1975 from biopsy tissue of a squamous cell carcinoma.

The histopathology of the surgical specimen was determined to be grade IV carcinoma.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)

Subculturing

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.Subcultivation Ratio: A subcultivation ratio of 1:2 is recommendedMedium Renewal: 2 to 3 times per week

Cryopreservation

culture medium 95%; DMSO, 5%

Culture Conditions

Temperature: 37.0°C

Atmosphere: air, 100%

STR Profile

Amelogenin: X

CSF1PO: 11

D13S317: 8

D16S539: 11

D5S818: 11

D7S820: 11,12

THO1: 8

TPOX: 11

vWA: 16

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 2

PGM1, 1

PGM3, 2

Name of Depositor

A Leibovitz

Deposited As

Homo sapiens

Passage History

A frozen ampule at passage 15 was received at the ATCC in January, 1982.

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.