My experience with MOPS buffer is that it eventually turns yellow,
regardless of whether you autoclave it or not. It just goes faster if
you autoclave it. I've also not seen a lot of differences between using
freshly made (clear/v. pale yellow) and older (gold) MOPS, at least in
Northerns.
On 19 Jun 1995, Daniel Solaiman wrote:
> >As far as MOPS buffers, don't repeat my mistake. Yes, you can DEPC-treat
> >MOPS, but when you autoclave it, it breaks down and turns that evil yellow
> >color that indicates it has gone bad. Therefore, make MOPS up with DEPC-
> >water but do not autoclave it.
>>> I recall reading somewhere that MOPS turns golden-yellow color when autoclaved
> -- regardless of prior direct treatment with DEPC or not. Anybody has any
> comments? I myself directly treat 10xMOPS with DEPC followed by autoclaving.
> Both rRNA bands and RNA size-markers appeared intact in gel visualized by EtBr
> staining.
>>>>>