Insulin Detection

Insulin is produced in a step-wise process. Preproinsulin is a biologically inactive precursor that consists of an N-terminal signal peptide followed by the proinsulin molecule. Removal of the signal peptide by signal peptidases results in proinsulin alone. Proinsulin contains the A-chain, B-chain, and a 31-amino-acid Connecting peptide or C-peptide. The C-peptide is positioned between the A-chain and the B-chain and functions to connect them. The C-peptide is removed once proinsulin is processed into insulin.

Antibodies for Insulin Detection

Human insulin monoclonal antibody may be used for the analysis of the structure, function, and metabolism of insulin. This anti-insulin antibody, also known as INS antibody, is applicable for Western blot (WB) analysis under non-reducing and non-heating conditions and for immunohistological (IHC) studies of frozen tissue sections.

Antibodies to the proinsulin C-peptide (also known as insulin C) are frequently used to study the structure, function, and metabolism of insulin as well as proinsulin. Insulin C antibodies can also be used for immunocytochemistry (ICC) and for immunohistochemical (IHC) studies of paraffin-embedded or frozen tissue sections. The following insulin C antibodies are available:

Features

IHCWBStructural, functional, and metabolic analyses of proinsulin and insulin

Human

(IS11-1) Mouse IgG1 MoAb

Cross‑reacts with bovine and porcine insulin

Insulin C(71–86)

M232

IHCStructural, functional, and metabolic analyses of proinsulin and insulin

Human

(h-Ins 1B-1)Mouse IgG2bMoAb

Does not cross‑react with human insulin A- and B-peptides, mouse C-peptide, or rat C‑peptide

Insulin C (71–84)

M233

IHCStructural, functional, and metabolic analyses of proinsulin and insulin

Mouse

Mouse (M-Ins 1J-4) Rat IgG2a MoAb

Does not cross-react with mouse insulin A- or B-peptidesDoes not cross-react with human C-peptide or rat C-peptide

Insulin C (71–84)

M178

IHCICCStructural, functional, and metabolic analyses of proinsulin and insulin

Mouse

(n/a) Guinea pig PoAb

Does not cross-react with human insulin C-peptide

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.