The Game Boy is Nintendo's second handheld console. The Game Boy is one of the best selling game systems of all time, selling around 64,420,000 units worldwide. It was so popular that Nintendo actually pushed back production of the Game Boy Color. It was discontinued in 1999 but is famous to collectors.

The Game Boy has the , , and buttons and the which make the controls similar to that on the NES. In 1995 a new version of the Game Boy, as part of Nintendo's "Play It Loud!" campaign, was released. This version is exactly the same as the original; the only difference is the color of the system. Game Boy games are also avalable on the Nintendo 3DS through the virtual console.

The Super Game Boy is not an actual system; rather, it is a Super Nintendo cartridge that one could insert Game Boy cartridges into, thus enabling people to play Game Boy games on their television screens through the Super Nintendo. Certain games have additional colors that can only be seen while played on a Super Game Boy. Donkey Kong has a special color palette designed for the Super Game Boy, as well as a special border designed to resemble the original arcade cabinet. Game Boy Color games with black cartridges can be played on the Super Game Boy.

The Game Boy Pocket is the second system of the Game Boy line. As the name implies, it is a pocket-sized Game Boy with its same features, and it was lighter in weight, with a clearer screen and a longer battery life. It requires two AAA batteries rather than four AA batteries in order to play games. The first release had no battery light indicator and it was rapidly replaced with a second release with the battery light due to customer complaints. The second release made its way into markets in several different colors (red, blue, green, yellow, black, etc.).

The Game Boy Light is the third system in the Game Boy line. It was released only in Japan in April 14, 1998 because Nintendo decided to make the Game Boy Color as Western gamers wanted color, not light, but said system was also released in Japan. The system is slightly bigger than a Game Boy Pocket and came only in two colors, gold and silver. Its main function, as the name implies, is the ability to produce backlight in order to play games in low light conditions. There were several special editions such as the Astro Boy edition.

At the time, it was the rarest Game Boy system only available in Japan, but due to online websites such as eBay the system became widely available in the entire world.

It was the only Game Boy system to have proper backlight until the release of the Game Boy Advance SP.

In the Game Boy comics mini-series by Valiant, Game Boys acted as gateways between the Mushroom World and Earth.

The Club Nintendo comic "Wart steht unter Strom" is about Wart (who is Bowser erroneously labeled as "Wart") who is playing Game Boy. The power of the batteries expires, so Wart carelessly throws the batteries away. Mario reminds him that they should be disposed of in containers instead.

In the Nintendo Adventure BookDoors to Doom, Mario and Luigi can encounter Wart, who is relaxing in his lair and playing a Game Boy, having mellowed out and become a skateboarder since his last encounter with the heroes.