Basic Protocol 11: Characterization of Antigen‐Specific VHHs

Materials

HBS‐E buffer: 10 mM HEPES (pH 7.4) containing 150 mM NaCl, and 3 mM EDTA (this buffer can be purchased from GE Healthcare;
if it is not purchased from GE Healthcare, it should be thoroughly degassed before use)

Nuttall, S.D., Krishnan, U.V., Hattarki, M., De Gori, R., Irving, R.A., and Hudson, P.J. 2001. Isolation of the new antigen receptor from wobbegong sharks, and use as a scaffold for the display of protein loop libraries. Mol. Immunol. 38:313‐326.