Journal: JPR:BioMedRx:An International Journal

Article Id:JPRS-PDRS-0000250Title:Assessment of the antioxidant activity of aqueous extract of Acacia catechu bark: An in vitro and in vivo studyCategory:Plant Drugs Related Study

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Safer antioxidants are essential to prevent the progression of free radical mediated disorders. Plants present a large source of natural antioxidants that might serve as leads for the development of novel drugs. The present study investigates the antioxidant properties of aqueous extract of bark of Acacia catechu in vitro and in vivo. The in vitro tests in diversified fields included total antioxidant activity, scavenging activities for DPPH and H2O2, reducing power and phenolic content. The IC50 values for DPPH and H2O2 scavenging were found to be 177.53 ± 12.35μg/ml and 455.2 ± 15.48 mg/ml respectively. The plant extract reduced the most Fe3+ ions in reducing power estimation. The extract yielded 67.40 ± 0.279 mg/ml gallic acid equivalent phenolic content per 100 mg plant extract. In the in vivo experiments, the extract treatment showed significant increase in the level of superoxide dismutase, catalase, glutathione-S-transferase and reduced glutathione at the dose of 100 and 200mg/kg bwt. The extract showed no toxicity, as the levels of the toxicity marker enzymes were found to be in the normal range. The present study provides evidence that aqueous extract of Acacia catechu bark shows potential antioxidant and free radical scavenging activity which might be due to the presence of phenolic compounds.