[Histonet] Re: Finding Lymph Nodes After Fixation

From:

rsrichmond@aol.com

Wanda Smith asks:
>>One of my pathologists asked me to ask a question - Has anyone had
success in finding colonic lymph nodes in fat AFTER
fixation with 10% neutral buffered formalin? This pathologist has tried
Dissect-aid and other solutions that seem to work on fresh fat, but he
has had no luck trying this after the fat has been in formalin.<<
No, this doesn't work. Though often when the specimen arrives in
formalin the fat has been so little penetrated by formalin that using a
disclosing fixative is still worthwhile.
Any other specialty, you'd be able to make arrangements with the
operating room staff to solve this problem. In pathology, the
red-haired stepchild again. And the clinical stakes are high here - one
positive lymph node, however small, requires chemotherapy.
Bob Richmond
Samurai Pathologist
Knoxville TN
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