Protein Regulation with Rapid Kinetics-ProteoTuner Systems

Rapidly regulate the quantity of your protein of interest, at the post translational level. The ProteoTuner systems use a powerful, unique technology to control your protein in living cells. ProteoTuner technology has been successfully used with cytosolic and membrane-bound proteins in a variety of cell types, organisms, and animal models.

One Vector/One Ligand System

A 12 kDa destabilizing domain (DD) that, when fused to a protein of interest, destabilizes the protein by targeting it for proteasomal degradation. Plasmid, retroviral and lentiviral PTuner vectors are available that carry the DD domain for fusion at either the N- or C-terminal of your protein.

A membrane-permeable small molecule (750 Da) ligand, Shield1, which protects the DD fusion protein from being degraded and allows it to accumulate in the cell. Stabilization of the DD-protein occurs in as little as 15–30 minutes.

We also offer the ProteoTuner Tag Kit, which provides you with a ProteoTuner tag sequence so you can quickly and easily convert any Living Colors fluorescent protein-C vector into a ProteoTuner vector. The Kit contains a ProteoTuner destabilization domain (DD) source vector plus a primer mix that lets you PCR-amplify the DD tag sequence. You can then use an In-Fusion Cloning Kit to insert the DD tag sequence upstream of the fluorescent protein coding sequence in any of our fluorescent protein-C vectors. The resulting vector will be able to express a protein of interest that is N-terminally-tagged with a fluorescent protein and subject to ligand-dependent degradation.

Gets rid of accumulated protein: On demand rapid degradation of accumulated protein is important for analysis of essential proteins

Applications

Protein function in pathways

Functional analysis of subunits of a protein complex

Functional analysis of essential proteins

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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