Anti-Golgi Complex antibody images

ICC/IF image of ab103439 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103439 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ab103439 staining Golgi Complex in murine spinal cord motor neurons by Immunohistochemistry (PFA perfusion fixed frozen sections).Tissue was fixed in paraformaldehyde, permeabilized using 0.2% Triton/PBS, blocked with 2.5% serum for 1 hour at 20°C and then incubated with ab103439 at a 1/500 dilution for 12 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.