Human telomeres are composed of long arrays of TTAGGG repeats that form a nucleoprotein complex required for the protection and replication of chromosome ends. TRF2 is ubiquitously expressed, bound specifically to duplex TTAGGG repeats in vitro, and localized to all human telomeres in metaphase chromosomes. It is expressed as 2 proteins of 65 and 69 kD. Affinity-purified antibodies specific for anti-TRF2 labeled the telomeres of intact human chromosomes, strengthening the correlation between occurrence of telobox and telomere-repeat recognition in vivo. TRF2 is likely to bind to double-stranded telomeric DNA as a homodimer.

性能

形式

Liquid

存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

存储溶液

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4

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纯度

Immunogen affinity purified

Primary antibody说明

Human telomeres are composed of long arrays of TTAGGG repeats that form a nucleoprotein complex required for the protection and replication of chromosome ends. TRF2 is ubiquitously expressed, bound specifically to duplex TTAGGG repeats in vitro, and localized to all human telomeres in metaphase chromosomes. It is expressed as 2 proteins of 65 and 69 kD. Affinity-purified antibodies specific for anti-TRF2 labeled the telomeres of intact human chromosomes, strengthening the correlation between occurrence of telobox and telomere-repeat recognition in vivo. TRF2 is likely to bind to double-stranded telomeric DNA as a homodimer.

靶标

功能

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.

ab4182 staining TRF2 in human Breast carcinoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton-x100 and blocking with 2% serum was performed for 30 minutes at 370C. Samples were incubated with primary antibody at 1/100 dilution in PBS for 2 hours at 37°C. An Alexa Fluor® 594 conjugated goat monoclonal to rabbit IgG was used at 1/75 dilution as secondary antibody.

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