Institute of Cell Biology National Academy of Sciences of Ukraine

Department of Molecular Genetics and Biotechnology

Research Grants

2008

International Projects:

INTAS project (Ref. No. 05-100000-7730) "Nonconventional yeasts: use of metabolic engineering approaches for construction of strains with improved alcoholic fermentation" (in collaboration with the Technical University of Aachen, Germany and the University of Lund,Sweden).

STCU project №3892 “Metabolic engineering of alcoholic fermentation of plant residues in non-conventional yeasts” in collaboration with the Institute of Applied Problems of Mechanics and Mathematics of NAS of Ukraine, Lviv).

STCU project №4378 "Gene and protein engineering of oxidoreductases for construction of bionanosized objects of analytical importance” (in collaboration with the Department of Analytical Biotechnology, Institute of Cell Biology, NAS of Ukraine).

Individual FEMS grant No. UA-SMU 2007-2 “Development of insertion mutagenesis for xylose fermenting yeast Pichia stipitisc for the identification of genes involved in the regulation of the ethanol synthesis“ (in collaboration with the University of Aachen, Germany).

Individual INTAS grant Ref. No. 06-1000014-6267 "Isolation of mutant forms of alcohol oxidase (mAOX) with improved bioanalytical characteristics and subsequent application of mAOX as bioselective element of an amperometric biosensor and scanning electrochemical microscopy" (in collaboration with the University of Bochum, Germany).

Individual FEMS grant No. UA-SMU 2008-2 "Study of a role of autophagic pathways in response to stress in the methylotrophic yeast Pichia pastoris and bakerʼs yeast Saccharomyces cerevisiae" (in collaboration with the University of Rzeszow, Poland).

Research project "Nanostructured systems for the design of anticancer drugs and ensuring their controlled pharmacokinetics (Comprehensive Program of Basic Research "Nanosystems, nanomaterials, nanotechnologies", Chapter 4. Bionanosystems, section 4.1. Bionanosystems and bionanomaterials for biology and medicine, artificial biomineralization nanomaterials).

Research project 25-08 "Construction and studying of Shewanella oneidensis strains expressing labeled hydrogenase” (Targeted Integrated Program of Fundamental Research of NAS of Ukraine "Fundamental Problems of Hydrogen Energy).

Research project "The portable analyzer based on amperometric enzyme biosensors for quality control of beverages in winemaking” (Comprehensive Scientific and Technical Program "Sensor systems for medical, environmental and industrial and technological needs“) (in cooperation with the Department of Analytical Biotechnology).

Within the framework of Targeted Comprehensive Program of Scientific Research NAS of Ukraine "Biomass as a fuel" ("Biofuels") 5 research projects are performed:

Development of methods for genetic engineering of ethanol tolerant yeast Brettanomyces bruxellensis to construct strains able to efficient alcoholic fermentation of sugars from plant biomass".

Metabolic engineering of the pentose phosphate pathway and transport of glucose and xylose for construction of termotolerant yeast strains able to efficient alcoholic fermentation of main sugars of plant residues.

Development of new methods for positive selection of ethanol overproducers from glucose, xylose and other sugars of lignocellulose

Construction of yeast strains accumulating acetaldehyde as a product of the conversion of sugars of plant biomass (lignocellulose).

Screening and selection of thermotolerant strains of Saccharomyces with increased ethanol-producing activity (in cooperation with the Department of Analytical Biotechnology).

Within the framework of research project "Development of advanced biomedical and bioengineering technologies for human health" (Program: "New medico-biological problems and human environment") three projects are performed:

Study of the regulation of glutathione biosynthesis in yeast for construction of overproducer of this tripeptide.

Project STCU №3892 “Metabolic engineering of alcoholic fermentation of plant residues in non-conventional yeasts "(in collaboration with the Institute of Applied Problems of Mechanics and Mathematics of NAS of Ukraine, Lviv).

Project STCU-4378 "Gene and protein engineering of oxidoreductases for construction of bionanosized objects of analytical importance” (in collaboration with the Department of Analytical Biotechnology, Institute of Cell Biology, NAS of Ukraine).

Within the framework of Targeted Comprehensive Program of Scientific Research NAS of Ukraine "Biomass as a fuel" ("Biofuels") 5 research projects are performed:

Development of methods for genetic engineering of ethanol tolerant yeast Brettanomyces bruxellensis to construct strains able to efficient alcoholic fermentation of sugars from plant biomass.

Metabolic engineering of the pentose phosphate pathway and transport of glucose and xylose for construction of termotolerant yeast strains able to efficient alcoholic fermentation of main sugars of plant residues.

Development of new methods for positive selection of ethanol overproducers from glucose, xylose and other sugars of lignocellulose.

Construction of yeast strains that accumulate acetaldehyde as a product of the conversion of sugars of plant biomass (lignocellulose).

Screening and selection of thermotolerant strains of Saccharomyces with increased ethanol-producing activity (in cooperation with the Department of Analytical Biotechnology).

Within the framework of research project "Development of advanced biomedical and bioengineering technologies for human health" (Program: "New medico-biological problems and human environment") three projects are performed:

Study of the regulation of glutathione biosynthesis in yeast for construction of overproducer of this tripeptide.

Project “Portable analyzer based on amperometric enzyme biosensors for quality control of beverages in the wine industry” according complex scientific and technical program of NAS of Ukraine "Sensor systems for medical, environmental and industrial and technological needs" (in cooperation with the Department of Analytical Biotechnology.

Construction of Saccharomyces cerevisiae yeast strains able to production of isobutanol as the main product of alcoholic fermentation.

Construction of yeast strains of Saccharomyces cerevisiae with a low content of ATP due to induction of futile cycles to improve the parameters of alcoholic fermentation.

Construction of thermotolerant Hansenula polymorpha yeast strains with increased expression of peroxisomal enzymes able to efficient conversion of xylose to ethanol.

Thermotolerant Saccharomyces yeast strains as producers of ethanol at elevated temperatures for the alcohol industry (together with the Department of Analytical Biotechnology).

Within the framework of Scientific Program "Fundamental bases of molecular and cell biotechnologies" 2 research projects are performed:

Molecular mechanisms of regulation of expression of genes involved in riboflavin biosynthesis and transport by flavinogenic yeasts.

Study of selective autophagy degradation of organelles and cytosolic proteins in the yeast Pichia pastoris and Saccharomyces cerevisiae.

Research project "The portable analyzer based on amperometric enzyme biosensors for quality control of beverages in winemaking” (Comprehensive Scientific and Technical Program "Sensor systems for medical, environmental and industrial and technological needs“) (in cooperation with the Department of Analytical Biotechnology).

2011

International Projects:

CRDF UKB-9047-LV-10 “Biotechnological production of Vitamin B2 (Riboflavin) based on yeasts”.

Ukrainian-Russian project number F40.4/006 "Molecular and genetic study of adaptation in Saccharomyces yeasts to elevated temperatures" in cooperation with the State Research Institute of Genetics and Selection of industrial microorganisms.

Program-targeted and competitive subjects of NAS of Ukraine:

Within the framework of a targeted complex program of scientific research NAS of Ukraine "Biomass as a fuel" ("Biofuels") 5 research projects are performed:

Ukrainian-Russian project N F40.4/006 "Molecular and genetic study of adaptation in Saccharomyces yeasts to elevated temperatures" (in collaboration with the State Research Institute of Genetics and Selection of Industrial Microorganisms).

Program-targeted and competitive subjects of NAS of Ukraine:

Within the framework of Scientific Program "Fundamental bases of molecular and cell biotechnologies" 2 research projects are performed:

Molecular mechanisms of regulation of expression of genes involved in riboflavin biosynthesis and transport by flavinogenic yeasts.

Study of selective autophagy degradation of organelles and cytosolic proteins in the yeast Pichia pastoris and Saccharomyces cerevisiae.

Within the framework of a targeted complex program of scientific research NAS of Ukraine "Biomass as a fuel" ("Biofuels") 5 research projects are performed:

Project “Scientific integration of the Polish-Ukrainian borderland area in the field of monitoring and detoxification of harmful substances in environment” IPBU.03.01.00-18-452/11-00 in the CBC Programme Poland-Belarus-Ukraine 2007-2013 (in collaboration with Rzeszow University, Poland).

Program-targeted and competitive subjects of NAS of Ukraine:

Within the framework of Scientific Program "Fundamental bases of molecular and cell biotechnologies" 2 research projects are performed:

Molecular mechanisms of regulation of expression of genes involved in riboflavin biosynthesis and transport by flavinogenic yeasts.

Study of selective autophagy degradation of organelles and cytosolic proteins in the yeast Pichia pastoris and Saccharomyces cerevisiae.

Within the framework of a targeted complex program of scientific research NAS of Ukraine "Biological resources and new technologies of bioconversion 3 research projects are performed:

Project "Scientific integration of the Polish-Ukrainian borderland area in the field of monitoring and detoxification of harmful substances in environment" IPBU.03.01.00-18-452/11-00 in the CBC Programme Poland-Belarus-Ukraine 2007-2013 (in collaboration with Rzeszow University, Poland).

Program-targeted and competitive subjects of NAS of Ukraine:

Within the framework of Scientific Program "Fundamental bases of molecular and cell biotechnologies" 2 research projects are performed:

Molecular mechanisms of regulation of expression of genes involved in riboflavin biosynthesis and transport by flavinogenic yeasts.

Study of selective autophagy degradation of organelles and cytosolic proteins in the yeast Pichia pastoris and Saccharomyces cerevisiae.

Within the framework of a targeted complex program of scientific research NAS of Ukraine "Biological resources and new technologies of bioconversion" 3 research projects are performed:

Project "Scientific integration of the Polish-Ukrainian borderland area in the field of monitoring and detoxification of harmful substances in environment" IPBU.03.01.00-18-452/11-00 in the CBC Programme Poland-Belarus-Ukraine 2007-2013 (in collaboration with Rzeszow University, Poland).

Program-targeted and competitive subjects of NAS of Ukraine:

Within the framework of a targeted complex program of scientific research NAS of Ukraine "Biological resources and new technologies of bioconversion" 3 research projects are performed:

Selection and construction of thermotolerant strains of Saccharomyces – producers of bioethanol using mutagenesis.

Within the framework of Scientific Program "Molecular and cellular biotechnology for medicine, industry and agriculture" 3 research projects are performed:

The genetic control of biosynthesis and transport of riboflavin in flavinogenic yeasts.

Application of metabolic engineering for construction of Saccharomyces cerevisiae strains capable of overproduction of glycerol under anaerobic conditions.

Mechanisms of degradation of peroxisomal and cytosolic proteins in methylotrophic yeasts and construction of overproducers of industrially valuable proteins on the basis of mutant strains with damage to these mechanisms.

Selection and construction of thermotolerant strains of Saccharomyces – producers of bioethanol using mutagenesis.

Within the framework of Scientific Program "Molecular and cellular biotechnology for medicine, industry and agriculture" 3 research projects are performed:

The genetic control of biosynthesis and transport of riboflavin in flavinogenic yeasts.

Application of metabolic engineering for construction of Saccharomyces cerevisiae strains capable of overproduction of glycerol under anaerobic conditions.

Mechanisms of degradation of peroxisomal and cytosolic proteins in methylotrophic yeasts and construction of overproducers of industrially valuable proteins on the basis of mutant strains with damage to these mechanisms.