Mutation details: This allele was generated by crossing mice carrying the Smad1tm2Rob allele to mice carrying a Cre transgene controlled by a mouse protamine 1 promoter that directs Cre-recombinase expression specifically to the male germline. An in vivo Cre-mediated excision removed all of the first coding exon and approximately 2kb of 5' and 3' flanking genomic sequences, and left a single loxP and EcoRI site in the locus. Northern analysis of 9.5dpc homozygous embryos using a probe for the 3' UTR detected a shorter transcript, but not the wild-type transcript. Western blot analysis using an antibody that recognizes the MH2 domain did not detect intact or truncated protein in 9.5dpc homozygous embryos.
(J:71998)