The Slow Death of Spontaneous Generation (1668-1859)

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The Slow Death of Spontaneous Generation (1668-1859)
Russell Levine and Chris Evers
From the time of the ancient Romans, through the Middle Ages, and until the late
nineteenth century, it was generally accepted that some life forms arose spontaneously
from non-living matter. Such "spontaneous generation" appeared to occur primarily in
decaying matter. For example, a seventeenth century recipe for the spontaneous
production of mice required placing sweaty underwear and husks of wheat in an openmouthed jar, then waiting for about 21 days, during which time it was alleged that the
sweat from the underwear would penetrate the husks of wheat, changing them into
mice. Although such a concept may seem laughable today, it is consistent with the other
widely held cultural and religious beliefs of the time.
The first serious attack on the idea of spontaneous generation was made in 1668 by
Francesco Redi, an Italian physician and poet. At that time, it was widely held that
maggots arose spontaneously in rotting meat. Redi believed that maggots developed
from eggs laid by flies. To test his hypothesis, he set out meat in a variety of flasks,
some open to the air, some sealed completely, and others covered with gauze. As he
had expected, maggots appeared only in the open flasks in which the flies could reach
the meat and lay their eggs.
This was one of the first examples of an experiment in the modern sense, in which
controls are used. In spite of his well-executed experiment, the belief in spontaneous
generation remained strong, and even Redi continued to believe it occurred under some
circumstances. The invention of the microscope only served to enhance this belief.
Microscopy revealed a whole new world of organisms that appeared to arise
spontaneously. It was quickly learned that to create "animalcules," as the organisms
were called, you needed only to place hay in water and wait a few days before
examining your new creations under the microscope.
The debate over spontaneous generation continued for centuries. In 1745, John
Needham, an English clergyman, proposed what he considered the definitive
experiment. Everyone knew that boiling killed microorganisms, so he proposed to test
whether or not microorganisms appeared spontaneously after boiling. He boiled chicken
broth, put it into a flask, sealed it, and waited - sure enough, microorganisms grew.
Needham claimed victory for spontaneous generation.
An Italian priest, Lazzaro Spallanzani, was not convinced, and he suggested that
perhaps the microorganisms had entered the broth from the air after the broth was
boiled, but before it was sealed. To test his theory, he modified Needham's experiment he placed the chicken broth in a flask, sealed the flask, drew off the air to create a
partial vacuum, then boiled the broth. No microorganisms grew. Proponents of
spontaneous generation argued that Spallanzani had only proven that spontaneous
generation could not occur without air.
The theory of spontaneous generation was finally laid to rest in 1859 by the young
French chemist, Louis Pasteur. The French Academy of Sciences sponsored a contest
for the best experiment either proving or disproving spontaneous generation. Pasteur's
winning experiment was a variation of the methods of Needham and Spallanzani. He
boiled meat broth in a flask, heated the neck of the flask in a flame until it became
pliable, and bent it into the shape of an S. Air could enter the flask, but airborne
microorganisms could not - they would settle by gravity in the neck. As Pasteur had
expected, no microorganisms grew. When Pasteur tilted the flask so that the broth
reached the lowest point in the neck, where any airborne particles would have settled,
the broth rapidly became cloudy with life. Pasteur had both refuted the theory of
spontaneous generation and convincingly demonstrated that microorganisms are
everywhere - even in the air.
Louis Pasteur Excerpt (1822-1895)
http://science.howstuffworks.com/dictionary/famous-scientists/chemists/louis-pasteur-info.htm
In 1849, Pasteur became a chemistry professor in Strasbourg, France, where he began
studying fermentation, a chemical process that breaks down organic materials. Such
microbes as bacteria, molds, and yeasts carry out this process, and humans long ago
learned to use this chemical process—well before they understood it. For example,
yeast breaks down sugar obtained from malted grain into ethyl alcohol and carbon
dioxide gas for use in beer. Sugar from grape juice is broken down in the same way for
use in wine. Fermentation also is essential in the production of bread, cheese, and
yogurt. Fermentation can also be unhealthy, such as when it turns milk sour.
Pasteur observed reproduction and growth in microorganisms, specializing in
bacteriology (the study of bacteria). In the late 1600's, Anton van Leeuwenhoek, a
Dutch amateur scientist, had recorded the first clear descriptions of bacteria. Using his
simple microscopes that were among the most powerful of his day, in 1674,
Leeuwenhoek concluded that the moving objects he viewed through his microscopes
were tiny animals, which he called animalcules. They included what are now known as
bacteria, protozoa, and rotifers. Leeuwenhoek hoped that his discovery of the
animalcules would disprove the widely believed theory of spontaneous generation. This
theory holds that certain forms of life, such as flies, worms, and mice, can develop
directly from nonliving things, such as mud and decaying flesh. Italian biologist
Francesco Redi in 1668 demonstrated that maggots (the young of flies) did not appear
in meat if he kept adult flies away from it. Before that, many people had believed that
flies developed directly from decaying meat.
Pasteur became the first to show that living things come only from living things. He
demonstrated that the microorganisms would grow in sterilized broth only if the broth
was first exposed to air that contained their spores (reproductive cells). Pasteur's
discoveries led to the development of the cell theory of the origin of living matter. The
cell theory states that all life originates from preexisting living material. Pasteur also
showed that although bacteria live almost everywhere, their spread can be controlled.
Questions:
1. How did the experiments of each scientist build on the thinking of the last?
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2. How did the background knowledge about bacteria change the way spontaneous
generation was explored? What does this say about the importance of
background information?
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