dNTP Set, PCR Grade is a complete set of individual, highly pure dNTPs. Each individual 100 mM dNTP is supplied in water and can be diluted and mixed with any other dNTP to the desired concentration. dNTP Mix, PCR Grade contains premixed dATP, dCTP, dGTP, and dTTP in water (pH 7.5), each at a concentration of 10 mM. Highly pure dNTPs are important for successful PCR, as the presence of contaminating impurities in PCR can result in a decrease in amplification sensitivity and product yield.

The performance of each lot of dNTP Set and Mix, PCR grade, is tested in a specific polymerase chain reaction requiring all four nucleotides.

Principle

Highly pure dNTPs are a crucial requirement for successful PCR, as the presence of contaminating impurities in PCR can result in a decrease in amplification sensitivity and product yield. Due to their high purity, dNTP Set and dNTP Mix, PCR Grade, are highly suitable for a variety of robust and sensitive PCR techniques.

Procedure

Protocols for the use of dNTP Set and Mix can be found in the appropriate QIAGEN PCR Kit. Selected kit handbooks can be downloaded from our Web site.

Applications

dNTP Set and dNTP Mix, PCR Grade are suitable for use in all standard PCR techniques and all sensitive PCR techniques. In addition, these products are ideal for use in combination with all common PCR and RT-PCR enzymes, including Taq DNA Polymerase, HotStarTaq DNA Polymerase, and our reverse transcriptase enzymes.

dNTP Set and dNTP Mix, PCR Grade can be used for applications such as: