Profinia Protein Purification System Buffers and Reagents

Overview

The Profinia control lysate is a lyophilized, dual-tagged 51 kD target protein that is meant to eliminate concerns over variability in purification buffer solutions or in the purification matrix itself. The control lysate facilitates system setup and initial purification and is included in every Profinia IMAC and GST starter kit.

Powdered urea and glutathione reagents are available for use with denaturing IMAC and GST applications, respectively. The urea packs are ideal for solubilizing and denaturing proteins in E. coli extracts. The glutathione pack is essential for eluting GST fusion proteins from immobilized glutathione resins.

Histidine (His)-tagged and GST-tagged monoclonal antibodies are used to detect target protein expression of overexpressed 6x His and GST fusion proteins. They are supplied at a concentration of 1 mg/ml in phosphate buffered saline, pH 7.4 with 0.05% NaN3.

Individually packaged reagents are also available for use with the Profinia system. While designed specifically to work with the Profinia system, these reagents also have general applicability within all stages of a purification workflow, from sample preparation to protein detection.

Profinia Control Lysate The Profinia control lysate is a lyophilized, dual-tagged 51 kD target protein that is meant to eliminate concerns over variability in purification buffer solutions or in the purification matrix itself. The control lysate facilitates system setup and initial purification and is included in every Profinia native IMAC and GST starter kit.

Urea and Glutathione Reagents Powdered urea and glutathione reagents are available for use with denaturing IMAC and GST applications, respectively. The urea packs are ideal for solubilizing and denaturing proteins in E. coli extracts. The glutathione pack is essential for eluting GST fusion proteins from immobilized glutathione resins.

His and GST Antibodies Histidine (His)-tagged and GST-tagged monoclonal antibodies are used to detect target protein expression of overexpressed 6x His and GST fusion proteins. They are supplied at a concentration of 1 mg/ml in phosphate buffered saline, pH 7.4 with 0.05% NaN3.

What is the education discount policy?

For more than 15 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows high school and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices.

How do I apply for the education discount?

If you are an educator at the high school or college level, visit our Education Discount Policy page to establish an education account number.