To explore the relationship between a classic assay for mitochondrial function (lactate/pyruvate ratio) and the real-time duplex NASBA method, we cultured control human fibroblasts (purchased from the Corriell Cell Repository, Camden, NJ) in DMEM supplemented with 100 mL/L FCS and antibiotics in the presence of the antiretroviral drug zalcitabine (ddC; 30 [micro]mol/L) for 4 weeks.

Data from transcripts were coded, first, for use of medication--whether people reported taking any antiretroviral medication (such as AZT, didanosine [ddI], or zalcitabine [ddc]), protease inhibitors, or preventive medication for opportunistic infections such as PCP.

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