Materials

Basic Protocol 1: Incubation of Cells with Sugar Analogs

Materials

Stock solutions (10 mM in ethanol or DMSO) of sugar analogs shown in Figure [Ac5ManNTGc (1), Ac4ManNAc (3), Ac4ManNProp (4), and Ac4ManNGc (5)], or other analogs of choice; if a free hydroxyl monosaccharide (e.g., ManNAc (2) or ManNLev; Mahal et al., ) is used in any experiment, a 500 mM stock solution should be prepared in PBS or serum‐free culture medium and filter sterilized