Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

WB

Use at an assay dependent concentration.

ICC

Use at an assay dependent dilution.

ICC/IF

Use at an assay dependent concentration. Used at a dilution of 1/50 for 18 hrs on mouse mammary cells (see Abreview for further information).

Target

RelevanceBeta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.
Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.

The Mouse monoclonal [BG-02] to beta Galactosidase, ab1047, should be able to detect the beta Galactosidase with the same sequence as that expressed in E. coli. This should work whether the protein is overexpressed or not. It should also work with beta...

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This antibody shows no cross-reactivity with other E. coli proteins and can be used for identification and characterization of beta-galactosidase fusion proteins expressed in lambda expression vectors. It was successfull...

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I am sorry I can not answer the question; however, I did find the following information in a product specific publication for this clone by Slavickova et al., PubMed ID 1284241.
In this publication I have found these ...

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To our knowledge, our beta Galactosidase antibody [BG-02]has yet to be tested by immunohistochemistry or on guinea pig tissue. All tested applications are specified on Abcam product datasheets.
Should you require fur...

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