The extended-spectrum β-lactamases (ESBLs) are the enzymes which degrade oxyimino-cephalosporins such as cefotaxime and ceftazidime, and make the antibiotics ineffective. In the past decade, drug resistance derived from Extended-spectrum β-lactamase-producing Escherichia coli (ESBL E. coli) has been increasing dramatically worldwide. The ESBLs genes are located on plasmids that can be easily transferred between and within bacterial species. It is indicated the linkage of ESBL E. coli from the food producing meats and the clinical samples. The presence of ESBL genes has been clearly documented in chickens. In this study, 27 raw chicken livers acquired from a supermarket in Niigata were used for detecting ESBL E. coli. E. coli was identified by DHL and chromogenic agar results as 19 of 27 (70.3%) samples. Antibiotic susceptibility test identified 6 of 19 (31.5%) ampicillin resistant E. coli samples and 1 of 19 (5.2%) ESBL E. coli sample. With genotyping result, an ESBL E. coli isolated in this study was confirmed as CTXM2.