Conventional "Click Chemistry" requires the presence of a Cu(I) catalyst that is toxic to most organisms and thus, prevents its use in many biological systems.

The novel Copper-free Click Chemistry is based on the reaction of a diarylcyclooctyne (DBCO) moiety with an azide-labeled reaction partner, known as strain-promoted alkyne azide cycloaddition (SPAAC). This new "Click Chemistry" is very fast at room temperature and does not require a cytotoxic Cu(I) catalyst. Diarylcyclooctynes are thermostable with very narrow and specific reactivity toward azides, resulting in almost quantitative yields of stable triazoles.

Cu(I)-free ligation reaction scheme: Diarylcyclooctyne-activated biomolecule A reacts with azide-activated biomolecule B without Cu(I) in aqueous conditions to form a stable triazole

Jena Bioscience's new product line for Copper-free Click Chemistry contains:

In addition, with our pre-made building blocks and in-house expertise we manufacture even the most exotic nucleotide analog from the mg to kg scale in a Custom Synthesis, at conditions that are affordable even for academic customers. Find more information in the PDF file below: