There are 2 sections to this exam: This section covers the last unitand another

section that covers recent ‘new’ material.

*You may access and complete review sections separately (taking one part and then studying for the ‘other section’)

*Both sections are to be completed closed-book, closed-notes with no prepared material of any kind.

*Similar to the rest of the semester, refer to the syllabus for a discussion of late penalties. There is no time limit for taking the review except for the overarching due date and time. Those are firm.

*All answers must be typed and in the form of complete sentences. Any figures or

graphs may be hand-drawn. This page must be the first page of your answer packet. *Fill out the information at the bottom and attach this page to the ones containing your answers. The top of each additional page in the packet should contain only your initials and the page number.

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Your review period begins when you read any question within this packet. Turn in this part of the review by handing it to me or placing it under my office door (Wat289). Fill in the sheet on my door to record when you turned in the exam. Please

staple all pages so that they do not become separated.

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There are a total of 100points on the exam. 50 of those points are on this section +++++++++++++++++++++++++++++++++++++++++++++++++++

Any questions about the review should be directed to me. I have no email access at home (evenings and weekends) During ‘business hours’ come by my office or contact me at kabernd or 894-2889 (o). Calls to my home must occur before 9:00pm 662-9744 Good luck!

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Name: ____________________________________

(PRINT)

Signature: _________________________________

My signature indicates that I have completed this review following the Honor Code.

This section of the exam was completed in ________hours.

I began it on ____ at _____ and completed it on ____ at ______.

Bio308 Cell Biology: Unit 3 Part of Final Fall 2006

DUE Before 9am Monday December 11th Fall 2000

Section II: One Scenario Parts 1-12(50pt)

Recent research has shown that compounds called polyphenols play a role in slowing down cellular aging. It would be exceedingly lucrative to extend these findings and develop a pill that will slow down or stop the aging process. Fortunately you head a cell cycle research laboratory at a large pharmaceutical company. You have characterized a hydrophilic extracellular substance that promotes cell viability. You develop a synthetic compound (called YNG) that mimics the natural molecule.

1)Using one sentence for each phase, describe the major phases of the cell cycle. (4pt) 2)An average cell spends a predominance of its life in one phase of the cell cycle. Name this phase and explain why the cell may spend so much time there. (4pt) 3)What is a CKI? What changes, if any, would occur in procession through the cell cycle if a specific CKI was perpetually present and active? (5pt)

To test your compound (YNG) you maintain petri dishes of human fibroblasts. You have a young technician convinced that you are omniscient because you are always able to predict exactly when the untreated fibroblast cultures will die. You enjoy the flattery but then explain to him how you know when newly isolated cultures will be needed because of the work of Hayflick.

4)What did Hayflick and his colleague do to try to get the scientific community to

believe their experiments about fibroblast lifespan? (4pt)

Because you have a voracious appetite for the current literature you are familiar with a hypothesis that links replication and aging. You have read many papers on the topic, including one written in 1997 by Austriaco, Jr. and Guarente.

5) What is the ‘end replication problem’ and why does it occur in somatic cells (like liver

7) What did Austriaco, Jr. and Guarente suggest about the role of telomere length in yeast lifespan. (5pt)

8) What organism do they (A, Jr. and G) use for their work? (Latin name genus and species) (2pt)

While you are contemplating these things your young technician comes to your office. He reports that, as hoped, adding YNG to fibroblasts causes the cells to live longer.

9) Provide three differences and one similarity between programmed cell death and necrosis. (4pt)

10) Given what you know about YNG and the pathways that lead to PCD/apoptosis describe a mechanism for how YNG could inhibit cell death. (4pt)

Bio308 Cell Biology: Unit 3 Part of Final Fall 2006

DUE Before 9am Monday December 11th Fall 2000

5 years later, YNG pills are undergoing clinical trials to test their safety and efficacy in humans. A representative from the AFCAD (Association for Curing Aging Diseases) approaches you. AFCAD is very interested in your work and the representative wants your expert opinion as to whether YNG will help patients with Werner’s syndrome.

11) What is Werner’s syndrome? (2pt)

12) Do you think that a patient with Werner’s syndrome would be helped by treatment with YNG? Why or why not? (6pt)

Bonus: 2pt. Complete the survey on the following pages. It is anonymous (don’t sign or

initial it). Place it in the folder on the bulletin board outside my office. If you choose to

complete the survey please indicate that you have done so on your answer sheet so that the survey can be anonymous but bonus points can be added to your review.

Bio308 Cell Biology: Unit 3 Part of Final Fall 2006

DUE Before 9am Monday December 11th Fall 2000

Cell Bio: Bio308 Exit Survey Fall 2006

Every year I make changes in the course and I value your input so that I can continue to make the course better. This is an anonymous exit survey that I have designed to ask course-specific questions that the general course evaluations can’t ask. If you choose to complete it please place it in the folder on the bulletin board outside my office.