The secreted recombinant mouse NOTCH1 (aa 1-526) consists of 518 amino acids and has a calculated molecular mass of 55 kDa. It migrates as an approximately 80 KDa band in SDS-PAGE under reducing conditions.

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.

Storage Buffer:

1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).

Storage Instruction:

Store at 4℃. After re-dissolution, aliquot and store at -80℃.

Application notes:

Western blot (WB): Use at an assay dependent dilution.Other Applications: Not tested.Optimal dilutions/concentrations should be determined by the end user.