Objective To establish an HPLC fingerprint of Songzhi Pill s and determine the content of the main difference component (emodin); T o provide the basis for the quality evaluation of Songzhi Pill s. Methods HPLC was used to detect 3 batches (a total of 60 samples) of Songzhi Pills. Similarity analysis was counted through TCM Chromatographic Fingerprint Similarity Evaluation System (V2.0) . Principal component analysis (PCA) and orthogonal partial least squares-discrimination analysis (OPLS-DA) were used to evaluate, find the difference components and detect their contents. Results The fingerprint of Songzhi Pills was established. 17 common peaks were obtained with similarities between 0.898 and 0.971. PCA and OPLS-DA could basically separate different batches of Songzhi Pills, and emodin was determined as the main different chemical component of Songzhi Pills . The contents of emodin in the three batches of Songzhi Pills were 0.013 5–0.017 1 mg/g, 0.036 7–0.043 6 mg/g, 0.022 1– 0.031 0 mg/g, respectively. Conclusion The HPLC fingerprint combined with PCA and OPLS-DA methods can quickly evaluate the quality of Songzhi Pills, providing references for quality control of Songzhi Pills.