Lentiviral Tet-On 3G Inducible Expression Systems

The Lenti-X Tet-On 3G Inducible Expression Systems combine the broad cellular tropism of lentiviral gene transfer technology with the power and versatility of our state-of-the-art tetracycline-regulated gene expression system. The lentiviral Tet-On-3G system consists of two lentiviral vectors: a regulator vector that stably expresses the Tet-On 3G transactivator protein, and a response vector that contains the TRE3G promoter that controls the expression of your transgene. Also included are a control response vector for luciferase expression, and our premium Lenti-X HTX Lentiviral Packaging System.

How it Works

Doxycycline-controlled “transactivators” are fusion proteins that contain a TetR-derived DNA binding domain joined to three minimal transcription activation domains from VP16 (Tet-On 3G). With very high specificity, the transactivator recognizes and binds to a Tetracycline Response Element (TRE) that is located in the PTRE3G promoter of pLVX-TRE3G, into which you clone your gene of interest. Binding of the transactivator to the TRE promoter results in strong activation of the downstream gene. Tet-On 3G is the premium 3rd generation version of the technology, with the tightest TRE promoter and most sensitive transactivator.

In Tet-On systems, gene expression is activated by the addition of Dox to the growth medium, while in Tet-Off systems, transcription is activated by its withdrawal.

High Titer Lentiviral Packaging

Clontech’s Lenti-X High Titer Packaging System, supplied with each system, generates vastly superior titers than most commercially available packaging systems. You can often transduce an entire dish of target cells with just 10–100 µl of unconcentrated viral supernatant. For more details, visit the Lenti-X HTX Packaging System product page.

Applications

Tetracycline-inducible expression in the widest range of hard-to-transfect cell types.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.