THYMUS DNA EXTRACTION

David B. Fankhauser, Ph.D.,Professor of Biology and ChemistryUniversity of Cincinnati Clermont College,Batavia OH 45103Modified from a protocol by Lana Hays

aliquot taken of
blended thymus

This page has been accessed times since 9
January 2003. 30 January 2001, 2 Feb 01, 16 Feb 01

Precipitated DNA
can be spooled

The stages of DNA isolation include
1) homogenization of thymus in a buffered isotonic
sucrose-detergent solution
2) increasing the osmolarity with saline
3) centrifugation to remove cellular debris
4) decantation of the supernatant into a clean beaker
5) precipitation of the DNA by layering cold ethanol
on the top of the supernatant
6) spooling of the precipitated DNA

Tried mushrooms-they formed a white band at the bottom of
theEtOH, just above the aqueous phase. In both cases, forgot to add the
detergent...The origianl recipe called for 30 g thymus/ 100 mL buffer,
but it formedso much DNA that it glommed up into "snot balls." 30
gm/100 mL of bufferwas probably too much thymus. Should try liver since
thymus is hard tofind. (Amy got it frozen in Hyde Park for $10/pound.)