Application Information: MEK7 (phospho Ser271/Thr275) antibody

We recommend the following starting dilutions: For WB: Use at a concentration of 0.1-1.0 μg/mL.Optimal dilution should be determined by the end user.

Positive Controls

Fusion protein expressing MEK 7

Concentration

0.5
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Immunogen affinity purified

Purification Note

Purified from rabbit serum by sequential epitope specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated MEK

Specifications: MEK7 (phospho Ser271/Thr275) antibody

Full Name

mitogen-activated protein kinase kinase 7

Background

Mitogen Activated Protein Kinase Kinase 7 (MEK 7 or MKK 7) is a 38 kDa member of a tyrosine/threonine protein kinase family that activates the c Jun NH2-terminal kinases (JNK), which is part of the inflammation/stress signaling pathway. Phosphorylation of MEK 7 by MEKK 1 on serine 271 and threonine 275 in the catalytic domain activates the protein and enables MEK 7 to phosphorylate JNK.

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

MEK7 (phospho Ser271/Thr275) antibody validated data

GTX24762 WB Image

Extracts prepared from background extracts with GST-tagged fusion protein expressing MEK7 added were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight

Extracts prepared from background extracts with GST-tagged fusion protein expressing MEK7 added were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight