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ABSTRACT: Peripheral blood mononuclear cells (PBMCs) from 13 vasculitis patients expressed varying gene profiles. A total of 13 sets of two color arrays were performed and each individual array included a target sample and a control sample.

Genopal™: a novel hollow fibre array for focused microarray analysis.

DNA research : an international journal for rapid publication of reports on genes and genomes 20101108 6

Expression profiling of target genes in patient blood is a powerful tool for RNA diagnosis. Here, we describe Genopal™, a novel platform ideal for efficient focused microarray analysis. Genopal™, which consists of gel-filled fibres, is advantageous for high-quality mass production via large-scale slicing of the Genopal™ block. We prepared two arrays, infectant and autoimmunity, that provided highly reliable data in terms of repetitive scanning of the same and/or distinct microarrays. Moreover, w ...[more]

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Project description:Peripheral blood mononuclear cells (PBMCs) from 13 vasculitis patients expressed varying gene profiles. A total of 14 sets of one color arrays were performed. Four technical replicates were performed for each sample.

Project description:Takayasu's arteritis (TA) Vasculitis (angiitis) is a chronic inflammatory disease involving large blood vessels. We here identify the genes whose mRNA levels are commonly augmented in the peripheral blood mononuclear cells (PBMCs) from TA patients regardless of the patient's symptom, active or inactive phase, compared with those of normal volunteers. Among them, ficolin 1 (FCN1) was notable because its mRNA level was more than 4.0 fold in all 8 patients examined. A total of 8 two color arrays were performed and each individual array included a target sample and a control sample.

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Project description:Peripheral blood mononuclear cells (PBMCs) from 13 vasculitis patients expressed varying gene profiles. Overall design: A total of 13 sets of two color arrays were performed and each individual array included a target sample and a control sample.

Project description:Peripheral blood mononuclear cells (PBMCs) from 13 vasculitis patients expressed varying gene profiles. Overall design: A total of 14 sets of one color arrays were performed. Four technical replicates were performed for each sample.

Project description:Objective: To optimize a strategy for identifying gene expression signatures differentiating SLE and anti-neutrophil cytoplasmic antibody-associated vasculitis that provide insight into the pathogenesis and identify biomarkers.<br/> Methods: Forty four vasculitis patients, 13 SLE patients and 25 age and sex-matched controls were enrolled. CD4 and CD8 T cells, B cells, monocytes and neutrophils were isolated from each patient and, together with unseparated peripheral blood mononuclear cells (PBMC), were hybridised to spotted oligonucleotide microarrays. <br/> Results: Using expression data obtained from purified cells we identified a substantial number of differentially expressed genes that were not detectable in the analysis of PBMC. Analysis of purified T cells identified an SLE-associated, CD4 T cell signature consistent with type 1 interferon signalling driving the generation and survival of tissue homing T cells and thereby contributing to disease pathogenesis. Moreover, hierarchical clustering using expression data from purified monocytes provided significantly improved discrimination between the patient groups than that obtained using PBMC data, presumably because the differentially expressed genes reflect genuine differences in processes underlying disease pathogenesis. <br/> Conclusion: The analysis of leucocyte subsets enabled the identification of gene signatures of both pathogenic relevance and with better disease discrimination than those identified in PBMCs. Thus, this approach provides substantial advantages in the search for diagnostic and prognostic biomarkers in autoimmune disease.

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