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"Excellent improvement to the original Gilson Microman; particularly in user comfort (fits more easily in the hand, better suited to extended use). The new volume lock is also very good, stops any drift while in use."

7500 Fast Real-Time PCR System

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The Applied Biosystems 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes. Specially designed Fast block ensures thermal uniformity at top speeds Faster ramp rates enable rapid results without compromising extension times or assay quality Fast optical plates ensure excellent precision in 10-30 uL reaction volumes Use TaqMan Fast Universal PCR Master Mix to run multiple Fast assays on one plate Finish more runs per day with the excellent performance equivalent to that offered by the 7500 System High Resolution Melting Analysis now Available The Applied Biosystems High Resolution Melting (HRM) Software is the easiest to use melting analysis software enabling r...Read more

Application Area: We are using for cellular gene expression for quantitative analysis of various RNA.

7500 Fast Real-Time PCR System

"The 7500 FAST system is easy to use, give reliable and reproducible results. The SDS software is easy to use and is really good for data analysis. Both Standard and Fast cycles can be used depending on the experimental needs. To reduce cost, PCR volume can be reduced to 10 ul. I used recommended master mixes for this machine and have always obtained good and reliable results. "

"This product is versatile and has a large throughput for a real-time PCR platform. It allows for multiplex reactions for several different targets in the same reaction well. It is easy to program and gives results in a time frame which is beneficial for the patient."

Application Area:Quantitative Detection of Hepatitis and Retroviruses Such as HCV

7500 Fast Real-Time PCR System

"The Applied Biosystems 7500 PCR cycler is very easy to use and it takes approximately 2 hours 19 minutes to complete a run. The software is very easy to link to our computers and links to databases where your patient’s files are. The cyclers have a very long life and my laboratory has not had any problems with their effectiveness or fitness for purpose. The quality of the PCR products from the cycler is very high plus contamination is significantly reduced compared to other cyclers which have also been used by the laboratory and other units in the department. It is very sensitive and in terms of training to be deemed competent to use the cycler, it is relatively easy from an operational level. The engineers are very quick to respond to any queries."

"It is very easy to use after the instructions from the application specialist. In the “fast PCR” mode you have the data after 40 min. The after sales service is very good and helps even with small problems. The Software for the instrument is easy to use and you can install it on more than one computer."

"Very intuitive-- easy to use and to find and label wells with different reporters. It's also very easy to customize and save templates. Machine plates can be switched out from 96 well to 384 well plates very easily. Temperature gradients are very useful for optimization."

"The machine itself is easy to use and produces good results. However, we have encountered problems with the machine losing connection with the computer since we purchased this equipment 2 years ago. This results in whole data sets being lost, all the calibrations needed resetting and a lot of down time waiting for engineers to come in. Applied Biosystems upgraded the computer they supplied with the machine less than a year after we had it in a hope to negate these connectivity issues. This worked for about 6 months, but now they've started up again. So I'm generally not particularly impressed with the system as a whole in terms of reliability."

"it is really easy to use and the software is friendly to use for people without training for most of the analysis."

Description

The Applied Biosystems 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes. Specially designed Fast block ensures thermal uniformity at top speeds Faster ramp rates enable rapid results without compromising extension times or assay quality Fast optical plates ensure excellent precision in 10-30 uL reaction volumes Use TaqMan Fast Universal PCR Master Mix to run multiple Fast assays on one plate Finish more runs per day with the excellent performance equivalent to that offered by the 7500 System

High Resolution Melting Analysis now AvailableThe Applied Biosystems High Resolution Melting (HRM) Software is the easiest to use melting analysis software enabling real-time PCR melt curve assays to be used more accurately for mutation scanning and genotyping. The HRM Software is available on the 7500 Fast Real-Time PCR System, which delivers precise results in a standard 96-well format.

Use the Applied Biosystems High Resolution Melting (HRM) Software to perform more sophisticated melting analysis with an easy to follow workflow and minimal subjective data analysis steps. Shortens analysis time by auto-calling genotypes and automatically omitting the no template controls Minimizes subjective analysis by automatically grouping unknown variant clusters No temperature shift required – distinguish between homozygous mutants and wild type more easily Allows easy data review with customizable multi-plot views, expandable windows and one-click color assignment to highlight curves of interest Facilitates data presentation with the option to export data and graphs directly to PowerPoint or as JPEG files