Mouse anti Human C4c antibody, clone 10-12 recognizes complement component 4c (C4c). The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b’s alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonisation and activation of the classical pathway. iC4b is further degraded into the inactive fragments C4d and C4c. C4c consists of two a-chain fragments, alpha 4 (13 kDa) and alpha 3 (25 kDa) linked to a beta chain through disulphide bridges and a gamma chain. It is expressed in the blood plasma.

Product Details

Target Species

Human

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Borate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide (NaN3)

Immunogen

Purified human C4c.

Approx. Protein Concentrations

1.0mg/ml

Storage Information

Storage

Store at +4oC or at -20oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

Please see label for expiry date.

More Information

Applications of C4c antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name

Verified

Min Dilution

Max Dilution

ELISA 1

Flow Cytometry

Immunohistology - Frozen

Western Blotting

1Clone 10-12 binds both C4c and C4 and so is not suitable for all ELISA applications.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.