CycleScript Reverse Transcriptase

Get more cDNA in less time with CycleScript Reverse Transcriptase. CycleScript is a versatile reverse transcriptase - applicable to both conventional Reverse Transcription and Cyclic Reverse Transcription (Cyclic RT, patent pending – for cDNA amplification). It features high activity across a wide range of temperatures from 37 to 55°C therefore, reverse transcription is carried out like PCR. The Cyclic RT reaction is composed of the following steps: 1st incubation at 15~40°C for primer annealing, heating up to 42~48°C for extension, and finally incubation at 50~55°C for denaturation of the secondary structure of the RNA (optional). Bioneer's novel Cyclic RT system offers homogeneous cDNA synthesis, with a high yield of cDNA up to 9 kb.

Features and Benefits

Broad range of working temperatures: For G:C rich RNAs or RNAs with significant secondary structure

Sensitive: Even the rarest transcript can be reliably made into cDNA

High yield of cDNA: For genes up to 9 kb within 10 minutes

RNase, DNase, and Proteinase-free: Ensures the integrity of your samples

One unit is defined as the amount of enzyme required to incorporates 1 nmole of dTTP into acid-precipitable material in 10 minutes at 37°C using poly (A)ooligo (dT) as template primer.

Figure 1.Comparison of transferrin receptor gene amplification with different reverse transcriptases.
700 ng of total RNA was used for reverse transcription and the same amount of amplified products were used for electrophoresis.

Figure 2.Comparison of β-actin gene amplification with different reverse transcriptases.
Each 10 ng, 1 ng, 100 pg, and 10 pg of total RNA was used for reverse transcription and the same amount of amplified products were used for electrophoresis.

Figure 3. Comparison of GAPDH gene amplification with different reverse transcriptases.
Each 10 ng, 1 ng, 100 pg, and 10 pg of total RNA was used for reverse transcription and the same amount of amplified products were used for electrophoresis.

Figure 4. Working temperature comparison of different reverse transcriptases.
Each 10 ng, 1 ng, 100 pg, and 10 pg of total RNA was used for reverse transcription and the same amount of amplified products were used for electrophoresis.