应用

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用

Ab评论

说明

WB

1/500 - 1/1000. Predicted molecular weight: 132 kDa.

ELISA

1/10000.

IHC-P

Use at an assay dependent concentration.

ICC/IF

Use a concentration of 1 - 5 µg/ml.

靶标

功能

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.

疾病相关

Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].

序列相似性

Contains 1 IRS-type PTB domain.Contains 1 PH domain.

翻译后修饰

Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.Phosphorylation of Tyr-896 is required for GRB2-binding.

ICC/IF image of ab52167 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52167, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Thank you for your enquiry. I am sorry to hear this vial of ab52167 is giving you trouble. We don't have a specificIHC-P protocol available for this antibody. But If you could, please complete the formbelow so I can get an idea of your protocol and dat...

Thank you for your call today and for letting us know about the problem with ab52167.
As we discussed, I am sending a vial of ab653 and I expect it to arrive next week. I found out that we don't ship to Canada on Saturdays, so the order will go ...