Recombinant Human IL2 (Ala21 ­ Thr153, with an N­terminal Met) was expressed in E. coli and produced using non­animal reagent in an animal­free laboratory.

Description:

Interleukin­2 (IL­2) is a O­glycosylated, four μ­helix bundle cytokine that has potent stimulatory activity for antigen­activated T cells. It is expressed by CD4+ and CD8+ T cells, μ? T cells, B cells, dendritic cells, and eosinophils. IL­2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL­2 and IL­2 Rμ synthesis. It contributes to T cell homeostasis by promoting the Fas­induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes. IL­2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells. Thus, IL­2 may be a key cytokine in the natural suppression of autoimmunity.

Source:

E. coli

N­terminal Sequence Analysis:

Met

Molecular Mass:

15 kDa

Activity:

Measured in a cell proliferation assay using CTLL­2 mouse cytotoxic T cells. The ED50 for this effect is typically 0.075 ­ 0.375 ng/mL.

Endotoxin Leve:

<1.0 EU per 1 μg of the protein by the LAL method.

Purity:

>97%, by SDS­PAGE under reducing conditions and visualized by silver stain.

Formulation:

Lyophilized from a 0.2 μm filtered solution in PBS. See Certificate of Analysis for details.

Reconstitution:

Reconstitute at 200 μg/mL in sterile 10 mM Sodium Acetate, pH 4.0.

Shipping:

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.