Plasma zonulin levels in childhood nephrotic syndrome

Abstract

Objective: We conducted this study to test the hypothesis that plasma zonulin levels are elevated in pediatric patients with nephrotic syndrome compared to healthy controls. Study Design: Plasma zonulin levels were measured by ELISA in 114 children enrolled in the NEPTUNE study. Clinical and laboratory data were retrieved from the NEPTUNE database. Results: The median age of the patients was 10 (IQR = 5 to 14) years, 59 were male, 64 had minimal change disease, 47 focal segmental glomerulosclerosis, median eGFR was 96 (IQR = 80 to 114) ml/min/1.73 m2, and median urine protein:creatinine ratio was 0.5 (IQR = 0.1 to 3.4) (g:g). The plasma zonulin level was 14.2 ± 5.0 vs. 10.2 ± 2.5 ng/ml in healthy adults in a report using the same assay kit, P = 0.0025. These findings were confirmed in an independent cohort of children with nephrotic syndrome compared to healthy age-matched controls, P = 0.01. Zonulin concentrations did not differ in children with minimal change disease vs. focal segmental glomerulosclerosis, frequently relapsing vs. steroid-dependent vs. steroid-resistant clinical course, and were not influenced by the immunosuppressive treatment regimen. There was no relationship between plasma zonulin levels and the absolute or percentage change in proteinuria from enrollment until the time of the zonulin assay. Conclusion: Plasma zonulin levels are elevated in childhood nephrotic syndrome regardless of level of proteinuria or specific treatment. The cause of the high plasma zonulin levels and whether zonulin contributes to glomerular injury requires further study.

abstract = "Objective: We conducted this study to test the hypothesis that plasma zonulin levels are elevated in pediatric patients with nephrotic syndrome compared to healthy controls. Study Design: Plasma zonulin levels were measured by ELISA in 114 children enrolled in the NEPTUNE study. Clinical and laboratory data were retrieved from the NEPTUNE database. Results: The median age of the patients was 10 (IQR = 5 to 14) years, 59 were male, 64 had minimal change disease, 47 focal segmental glomerulosclerosis, median eGFR was 96 (IQR = 80 to 114) ml/min/1.73 m2, and median urine protein:creatinine ratio was 0.5 (IQR = 0.1 to 3.4) (g:g). The plasma zonulin level was 14.2 ± 5.0 vs. 10.2 ± 2.5 ng/ml in healthy adults in a report using the same assay kit, P = 0.0025. These findings were confirmed in an independent cohort of children with nephrotic syndrome compared to healthy age-matched controls, P = 0.01. Zonulin concentrations did not differ in children with minimal change disease vs. focal segmental glomerulosclerosis, frequently relapsing vs. steroid-dependent vs. steroid-resistant clinical course, and were not influenced by the immunosuppressive treatment regimen. There was no relationship between plasma zonulin levels and the absolute or percentage change in proteinuria from enrollment until the time of the zonulin assay. Conclusion: Plasma zonulin levels are elevated in childhood nephrotic syndrome regardless of level of proteinuria or specific treatment. The cause of the high plasma zonulin levels and whether zonulin contributes to glomerular injury requires further study.",

author = "{NEPTUNE Cohort Study} and Howard Trachtman and Gipson, {Debbie S.} and Lemley, {Kevin V.} and Troost, {Jonathan P.} and Christian Faul and Morrison, {Debra J.} and Vento, {Suzanne M.} and Ahn, {Dong Hyun} and Goldberg, {Judith D.} and J. Sedor and K. Dell and M. Schachere and K. Lemley and L. Whitted and T. Srivastava and C. Haney and C. Sethna and K. Grammatikopoulos and G. Appel and M. Toledo and L. Greenbaum and C. Wang and B. Lee and S. Adler and C. Nast and {La Page}, J. and A. Athavale and M. Itteera and A. Neu and S. Boynton and F. Fervenza and M. Hogan and J. Lieske and V. Chernitskiy and F. Kaskel and N. Kumar and P. Flynn and J. Kopp and E. Castro-Rubio and E. Brede and H. Trachtman and O. Zhdanova and F. Modersitzki and S. Vento and R. Lafayette and K. Mehta and C. Gadegbeku and D. Johnstone and Z. Pfeffer and D. Cattran",

year = "2019",

month = jan,

day = "1",

doi = "10.3389/fped.2019.00197",

language = "English (US)",

volume = "7",

journal = "Frontiers in Pediatrics",

issn = "2296-2360",

publisher = "Frontiers Media S. A.",

number = "MAY",

}

TY - JOUR

T1 - Plasma zonulin levels in childhood nephrotic syndrome

AU - NEPTUNE Cohort Study

AU - Trachtman, Howard

AU - Gipson, Debbie S.

AU - Lemley, Kevin V.

AU - Troost, Jonathan P.

AU - Faul, Christian

AU - Morrison, Debra J.

AU - Vento, Suzanne M.

AU - Ahn, Dong Hyun

AU - Goldberg, Judith D.

AU - Sedor, J.

AU - Dell, K.

AU - Schachere, M.

AU - Lemley, K.

AU - Whitted, L.

AU - Srivastava, T.

AU - Haney, C.

AU - Sethna, C.

AU - Grammatikopoulos, K.

AU - Appel, G.

AU - Toledo, M.

AU - Greenbaum, L.

AU - Wang, C.

AU - Lee, B.

AU - Adler, S.

AU - Nast, C.

AU - La Page, J.

AU - Athavale, A.

AU - Itteera, M.

AU - Neu, A.

AU - Boynton, S.

AU - Fervenza, F.

AU - Hogan, M.

AU - Lieske, J.

AU - Chernitskiy, V.

AU - Kaskel, F.

AU - Kumar, N.

AU - Flynn, P.

AU - Kopp, J.

AU - Castro-Rubio, E.

AU - Brede, E.

AU - Trachtman, H.

AU - Zhdanova, O.

AU - Modersitzki, F.

AU - Vento, S.

AU - Lafayette, R.

AU - Mehta, K.

AU - Gadegbeku, C.

AU - Johnstone, D.

AU - Pfeffer, Z.

AU - Cattran, D.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Objective: We conducted this study to test the hypothesis that plasma zonulin levels are elevated in pediatric patients with nephrotic syndrome compared to healthy controls. Study Design: Plasma zonulin levels were measured by ELISA in 114 children enrolled in the NEPTUNE study. Clinical and laboratory data were retrieved from the NEPTUNE database. Results: The median age of the patients was 10 (IQR = 5 to 14) years, 59 were male, 64 had minimal change disease, 47 focal segmental glomerulosclerosis, median eGFR was 96 (IQR = 80 to 114) ml/min/1.73 m2, and median urine protein:creatinine ratio was 0.5 (IQR = 0.1 to 3.4) (g:g). The plasma zonulin level was 14.2 ± 5.0 vs. 10.2 ± 2.5 ng/ml in healthy adults in a report using the same assay kit, P = 0.0025. These findings were confirmed in an independent cohort of children with nephrotic syndrome compared to healthy age-matched controls, P = 0.01. Zonulin concentrations did not differ in children with minimal change disease vs. focal segmental glomerulosclerosis, frequently relapsing vs. steroid-dependent vs. steroid-resistant clinical course, and were not influenced by the immunosuppressive treatment regimen. There was no relationship between plasma zonulin levels and the absolute or percentage change in proteinuria from enrollment until the time of the zonulin assay. Conclusion: Plasma zonulin levels are elevated in childhood nephrotic syndrome regardless of level of proteinuria or specific treatment. The cause of the high plasma zonulin levels and whether zonulin contributes to glomerular injury requires further study.

AB - Objective: We conducted this study to test the hypothesis that plasma zonulin levels are elevated in pediatric patients with nephrotic syndrome compared to healthy controls. Study Design: Plasma zonulin levels were measured by ELISA in 114 children enrolled in the NEPTUNE study. Clinical and laboratory data were retrieved from the NEPTUNE database. Results: The median age of the patients was 10 (IQR = 5 to 14) years, 59 were male, 64 had minimal change disease, 47 focal segmental glomerulosclerosis, median eGFR was 96 (IQR = 80 to 114) ml/min/1.73 m2, and median urine protein:creatinine ratio was 0.5 (IQR = 0.1 to 3.4) (g:g). The plasma zonulin level was 14.2 ± 5.0 vs. 10.2 ± 2.5 ng/ml in healthy adults in a report using the same assay kit, P = 0.0025. These findings were confirmed in an independent cohort of children with nephrotic syndrome compared to healthy age-matched controls, P = 0.01. Zonulin concentrations did not differ in children with minimal change disease vs. focal segmental glomerulosclerosis, frequently relapsing vs. steroid-dependent vs. steroid-resistant clinical course, and were not influenced by the immunosuppressive treatment regimen. There was no relationship between plasma zonulin levels and the absolute or percentage change in proteinuria from enrollment until the time of the zonulin assay. Conclusion: Plasma zonulin levels are elevated in childhood nephrotic syndrome regardless of level of proteinuria or specific treatment. The cause of the high plasma zonulin levels and whether zonulin contributes to glomerular injury requires further study.