Using the Animus 2.0, Desmond Miles attempted to relive one of the late memories of his ancestor, Ezio Auditore, hoping that it would reveal the location of his Apple of Eden, which could allow them to prevent the foretold disaster. However, instead of entering the memory set in 1506, he was forced into a different one, occurring during a battle in Viana. From there, as Ezio looked upon the besieged Viana Castle, he recalled the similar events of the siege of Monteriggioni, subsequently forcing Desmond back further, into a memory from 1499.

Mario Auditore then called down to him from the entrance, and he and Ezio fought their way out of the Vatican. Ezio, unable to decide whether or not to cast the Apple into the Tiber River, gave it to Mario for safe keeping. The two then rode back to Monteriggioni on horseback.

However, Ezio soon learned that the Templar threat had diminished little, when Cesare Borgia, son of Rodrigo Borgia, laid siege to Monteriggioni. Cesare's army of soldiers, towers, and cannons attacked in full force, destroying much of the city and villa. The attack ended with Monteriggioni in ruins, Ezio wounded badly by arquebusiers, and Mario killed by Cesare himself. Though Ezio attempted to follow his uncle's murderer on horseback, he passed out on the road to Rome from his injuries.

At that point, Desmond woke from the Animus stationed in the back of the Assassins' van, they having arrived at the Villa Auditore in modern Monteriggioni, their last safehouse in Italy. Lucy Stillman explained that they need to recover Ezio's Apple of Eden, as she suspected that Minerva had altered it somehow when she had touched it in the Vault.

Desmond, Lucy, Shaun and Rebecca then headed for the Villa, seeking the safety of the underground Sanctuary, which would keep them hidden from Abstergo's cell towers. However, as the door was locked, Desmond and Lucy left to discover another way in. After making their way through the secret passage that Ezio and the citizens had used to flee the Borgia attack, they opened the way for Shaun and Rebecca.
[1]Desmond reentering the Animus.Added by DdelaghettoInside the Sanctuary, Desmond saw an aged Ezio due to the bleeding effect, and deduced that he must have returned to the villa many years after the attack, though for an unknown purpose. He also found a symbol that Ezio had drawn on the wall and, using Eagle Vision, saw a series of numbers hidden underneath.

The four of them then set up the Animus 2.0, and Desmond returned to Ezio's memories, with Lucy believing that improving his synchronization with Ezio would allow Desmond to access the memory revealing the location of the Apple.

Ezio waking up after the siege.Added by Zwoooooosh:Main article: Liberation of Rome
Ezio regained consciousness in a small house in Rome, where the woman who had been tending to him told him that a man had brought him there, and had supplied him with new armor and clothing. After leaving the house and receiving medicine from a doctor, Ezio left to meet with Niccolò Machiavelli.

Through Machiavelli, he discovered that Rome was in disrepair, and that the citizens were being oppressed by the Borgia. Later, he would also stumble across the Followers of Romulus, and explore one of their lairs, Nero's Golden Palace.

Ezio then began to rebuild the Brotherhood by recruiting Apprentices, who were made up of both distressed citizens who wished to liberate their city, and young novice Assassins, such as Francesco Vecellio. After the recruits had completed their training, he placed them in teams and sent them on missions across Europe.

Ezio then began to break down the Borgia influence in Rome by destroying several Borgia towers and their captains. He also sabotaged Cesare's forces by striking at their arsenal, their military funding, and their support from the French forces. To do so, he destroyed the war machines of Leonardo da Vinci, and assassinated two of Cesare's key generals, his Banker, Juan Borgia, and the French general, Octavian de Valois.

Afterwards, he was informed that Pietro Rossi, an actor and Lucrezia Borgia's plaything, possessed a key to the Castel Sant'Angelo. Knowing that he would need it in order to assassinate Cesare and Rodrigo successfully, Ezio tailed after Micheletto Corella, Cesare's personal assassin, who had been sent to kill Pietro. After Ezio had rescued him, both from being stabbed with a spear during his play, and from the ailments of poison, Pietro handed the Assassin the key to the Castello.

Upon completion of these tasks, Ezio inducted Claudia into the Assassin Order, and was also elected by Machiavelli to be the Mentor of the Italian Assassins.

The Assassins standing against Cesare.Added by Master Sima YiSoon afterwards, Ezio infiltrated the Castel, where he witnessed the murder of Rodrigo by his son, Cesare. Cesare forced the location of the Apple of Eden from Lucrezia, and hurried to retrieve it from Basilica di San Pietro. However, Ezio successfully reached the Apple before Cesare, and subsequently used it to demilitarize the remnants of Cesare's army.

In one last battle, Ezio and his fellow Assassins fought Cesare and his surviving men at the gates of Rome. Cesare was arrested by Fabio Orsini by the order of the new Pope, though not before commenting that he would not be in chains for long and that he would never be killed by any man.

Sometime later, Ezio, troubled by Cesare's comments, discussed them with Leonardo, who suggested using the Apple to find out if what he said was true. Ezio was hesitant, but decided to take his advice, and discovered that Cesare would indeed free himself from prison. Ezio declared that he needed to leave at once, reassuring Leonardo's concerns for the Assassin Guild he was leaving behind by saying, "I built this Brotherhood to last, with or without me."

Ezio throwing Cesare off a wall.Added by Master Sima Yi:Main article: Siege of Viana
In 1507, Ezio finally tracked Cesare down at the Siege of Viana, Spain. Ezio fought through the infantry, and finally managed to corner Cesare on the castle wall. There, in a climactic battle, Ezio managed to destroy Cesare's armor and ultimately defeat him. Though Cesare insisted that he would not die in the hands of man, Ezio then left him to the "hands of fate," and threw him from the battlements to his death.

Desmond emerged from the Animus shortly after reliving this memory, listening to his teammates deducing a way to get into the Vault. Just as Rebecca mentioned that they needed a spoken password to enter it, a blackout occurred, cutting them off from using the Animus to find out. However, Desmond realized that the symbol he had seen on the door of the Vault was the same one he had seen drawn on the villa wall. Shaun discovered that the sequence of numbers hidden behind the symbol referred to the seventy-two names of God, setting the password to be 72.

The Assassins then drove to the Colosseo in Rome, and Desmond separated from the others to find a way into the Vault. As he traveled the underground passageway, he encountered another of Those Who Came Before, Juno, who spoke at length of the downfall of humankind.

Arriving inside the Santa Maria Aracoeli, Desmond unlocked the door for his teammates and, shortly afterwards, witnessed a memory of Ezio free-running through the church. He followed him and activated a system of bars and levers, finally causing a small pedestal to emerge from the floor. Placing his hand on it, Desmond activated the floor, lowering the four of them down to the door of the Vault.

Desmond spoke the password, and the door opened to reveal a large empty chamber, with a tall platform raised in the middle, upon which the Apple awaited. Desmond activated the staircase up to it by jumping across a series of pillars; while Juno's voice echoed through the cavern as she spoke cryptically of humans, the Pieces of Eden and a "sixth sense." However, Desmond was apparently the only one who could hear her.
[2]The Assassins inside the Vault.Added by Assassin-RayneOnce the Assassins had access to the platform, Desmond touched the Apple, and a series of holographic symbols appeared.

Shaun mentioned that two of the most distinct ones, the Phrygian Cap and the Masonic Eye, only came together in one place; however, when Desmond picked up the Apple, time seemed to stop and he found himself controlled by Juno. Though he struggled, she forced him to draw his hidden blade and stab Lucy in the abdomen. The two then fell to the ground unconscious, with blood pooling around Lucy.

Later, William Miles and another unknown male could be heard speaking, saying that Desmond had gone into shock. William ordered the other to put Desmond back into the Animus, despite the other's protests on how badly the Animus had affected Desmond already. Desmond had entered a coma, though William claimed that Desmond would live through it.

The tool selection HUB.Added by Ezio Auditore PS360The weapon wheel feature in Assassin's Creed: Brotherhood remains largely the same as in Assassin's Creed II, but with several new features, such as the addition of the Crossbow.

Assassin's Creed: Brotherhood is the first Assassin's Creed game to feature a multiplayer mode. In it, the players are Templars in training at the Abstergo facility, who use Animi and the Bleeding Effect to train in combat, stealth and free-running. The simulation consists of stalking and killing one another in areas set to mimic countries and cities of the Renaissance era.

Assassin's Creed: Brotherhood was developed by Ubisoft Montreal. Montreal also worked on both of the other main Assassin's Creed games in the series and was thus chosen to lead production for the third installment. A new Assassin's Creed episode featuring multiplayer was announced during Ubisoft's 2009 fiscal third quarter results while not revealing its name. In early May 2010, a GameStop employee published on the internet some images of a pre-order box featuring the Assassin's Creed: Brotherhood title while Ubisoft was teasing the game on Facebook and Twitter. Ubisoft then confirmed the authenticity of these pictures.[1]Brotherhood has not been numbered unlike Assassin's Creed II because players, and even developers themselves would have expected a new setting and a new ancestor while this is only the continuation of Ezio's story.

The game was developed primarily by Ubisoft Montreal in Canada. Production was aided in part by four other Ubisoft developers: Annecy, Singapore, Bucharest and Québec City. The multiplayer mode is mainly developed by Ubisoft Annecy, the studio responsible for creating multiplayer mode in Tom Clancy's Splinter Cell: Chaos Theory. Ubisoft also announced plans for downloadable content (DLC) after the game's launch. Two sets of free DLC have already been released under the names "Animus Project Update 1.0" and "Animus Project Update 2.0". The first includes the new map Mont Saint-Michel and one new mode, Advanced Alliance. The "Animus Project Update 2.0" was released in January 2011, was also free, and included another map, mode and the introduction of a player grading system. From the perspective of performance, Ubisoft have commented that they hope the gaps between the PlayStation 3 and Xbox 360 versions will be even smaller with Brotherhood.

While still in development, creative director Patrice Désilets left before the game's presentation at the E3 2010. Ubisoft and production manager Jean-Francois Boivin stated that he only took a "creative break" after completing his task on Brotherhood. A teaser trailer of the multiplayer mode was released on the official site before the E3. A cinematic debut trailer was diffused during Ubisoft's E3 2010 press conference along a walkthrough of the game's beginning. Assassin's Creed: Brotherhood reached gold status on October 28, 2010. The Microsoft Windows version uses Tagès copy protection, as well as Ubisoft online services platform, but doesn't require an always-on Internet connection to play. On November 30, 2010, a novelization of the game was published. The novel is a sequel to the previous novel.

The background of the game's title menu cycles between the blue of Abstergo's Animus (1.28), and the white of the Assassins' Animus 2.0.

In the multiplayer mode, despite all the characters being Templars and using Abstergo's Animi 1.28, the desynchronization animation and the blend animation are from the Animus 2.0.

Nolan North, the voice actor of Desmond Miles, had admitted to be working on a new Assassin's Creed game before any official announcement, and game developers said that his story would "take an interesting turn."

The covers of the Xbox 360 and PS3 versions of the game show Ezio with dual Hidden Blades in an "X" pointing upwards, while on the PC version cover, the blades are facing downwards.

As seen in this video, the PC version of the game is able to support 3 monitor gaming.