Figure 4.

Validation of EGR-1 enrichment by ChIP-real-time PCR analysis. PCR primers were designed
to 50 regions in selected clusters and 8 negative regions without enrichment in CpG
islands. Data are relative fold enrichments, calculated by determining the apparent
immunoprecipitation efficiency and normalized to the level observed at a control region
(mean ± standard deviation, n = 2).