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ESRP2 Antibody (22C5.H7.A6) Summary

Immunogen

Full length recombinant mouse Esrp-2 protein.

Specificity

A BLAST analysis was used to suggest cross-reactivity with Esrp-2 from mouse based on a 100% identity with the immunizing sequence. Cross-reactivity with Esrp-2 from other sources has not been determined.

Isotype

IgG1

Clonality

Monoclonal

Host

Mouse

Gene

ESRP2

Purity

Protein A purified

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Applications/Dilutions

Dilutions

Western Blot 1:1000

ELISA 1:100000

Application Notes

This protein-A purified antibody has been tested for use western blotting and shows specific reactivity by with ESRP2 transfected cell lysates.. Expect a band approximately 77.4 kDa in size corresponding - Esrp-2 by western blotting in the appropriate cell lysate or extract. The antibody shows bands at ~77kD and 70kD and no reactivity is observed in cells transfected with GFP or ESRP1. A starting dilution of 1:1000 is suggested for western blotting.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Purity

Protein A purified

Alternate Names for ESRP2 Antibody (22C5.H7.A6)

epithelial splicing regulatory protein 2

FLJ21918

FLJ22248

RBM35BRNA-binding protein 35B

RNA binding motif protein 35A

RNA binding motif protein 35B

RNA-binding motif protein 35B

Background

Epithelial splicing regulatory protein 2 (Esrp-2) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-2 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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