Re: [Histonet] Chatter

From:

Rene J Buesa

Heather:
First things first: is it true that your sections are chattered? How different they are from the other co-worker's sections?
I think that you should get slides made by you and by your coworker and take them to the pathologist so he can show you where your chatters are.
If you want to modify something you first have to know what to modify.
Chattaring has many causes and since it seems that yours are the only ones with chatter, we can eliminate the main cause that is overdrying of the small biopsies while being processed.
Really I think that this pathologist should indicate to you the specific areas he considers as being chattered. Remember that communication is the main avenue to tackle all problems between people.
After that you can begin fiddling with ice, blades, angles and the like.
Hope this will hekp you!
René J.
"Harper, Heather A., CIV" wrote:
I have worked with 8 pathologists and have never encountered this problem.
This particular pathologist, says that when I cut, my slides have chatter,
especially the small biopsies but particularly the intestinal biopsies. My
co-worker does the majority of the cutting, yet has never received a comment
about "chatter" on her cutting of slides from this pathologist. Could
somebody tell me what causes this chatter, especially with intestinal
biopsies? Is it the microtome, which I have a new knife holder, so I am good
to go there, because it cuts beautifully now. I have no problems getting
smooth paraffin sections. This pathologist is making me paranoid, because I
have never had this problem with any other pathologists. Thanks and Happy
Friday:-)
Heather a. Harper
Supervisor of Histology
Naval Hospital
Pensacola, FL
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