Available hosts

Available applications

Background of Collagen type XVII alpha 1 chain antibody

Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in the gene coding for collagen XVII are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD 1, is generated by proteolytic processing of the full length form. Two transcript variants, one resulting from alternative splicing in the 3' UTR, have been identified for this gene.

V-5-8 Monoclonal Antibody specifically binds to a 180 kDa protein by Immunoblotting: Different extracts of Human epidermis (Lanes 1-3) and primary Human keratinocytes (Lanes 4-11) were fractionated by 7.5% SDS-PAGE and immunoblotted with an 100-fold dilution of the Monoclonal antibody V-5-8. Molecular weight markers are depicted to the right.

COL17A1 Antibody (Center) (Cat. #AP51010PU-N) IHC analysis in formalin fixed and paraffin embedded skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COL17A1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.