Welcome to BioLegend’s Flow Cytometry Instrument Guide/Fluorophore Selector tool. Here, we provide a general view of which fluorophores can be used on commercially available instruments. As instrument configurations and laser options can vary widely, be sure to check with your own instrument to determine if the configuration matches those presented here or if your instrument can be modified/configured to accommodate certain fluorophores.

Exmax

Emmax

Alexa Fluor® 405

401

421

Brilliant Violet 421™

405

421

Pacific Blue™

410

455

BD Horizon™ V450

406

450

Brilliant Violet 510™

405

510

BD Horizon™ V500

415

500

AmCyan

458

489

Pacific Orange™

400

551

Brilliant Violet 570™

405

570

Brilliant Violet 605™

405

603

Brilliant Violet 650™

405

645

Brilliant Violet 711™

405

711

Brilliant Violet 785™

405

785

Alexa Fluor® 488

495

518

FITC (fluorescein)

493

525

PE (R-Phycoerythrin)

565

575

Cy3

514

566

PE/Cy5

565

670

PerCP

482

675

PerCP/Cy5.5

482

690

PE/CF594

565

610

PE/Dazzle™ 594

565

610

PE/Cy7

565

774

APC (allophycocyanin)

645

660

Alexa Fluor® 647

650

668

Alexa Fluor® 700

696

719

APC/Cy7

650

774

APC/Fire™ 750

650

787

APC/Alexa Fluor® 750

650

774

Hoechst 33342

350

450

DAPI

359

461

7-AAD

546

647

PI (Propidium Iodide)

540

620

To compare instruments, press and hold the Control key to make multiple selections, selecting all your desired manufacturers first, followed by desired instruments and lastly, the laser options for each instrument.