About this book

This textbook is the second edition of a bestselling title offering an instructive and comprehensive overview of our current knowledge of biocatalytic processes in an industrial setting. Each chapter has now a section with problems and solutions, new case studies have been introduced and a companion provides supplementary data in addition to all figures as teaching material. Of course, each chapter has been thoroughly updated and expanded where needed; the book contains now in addition to the new online material 15 % more printed content including some color figures. Following an introduction to the history of enzyme applications and the motivations for using these highly selective and environmentally friendly methods, the book covers enzyme mechanisms and kinetics, production, recovery, characterization and their design, including recombinant methods. Alongside the application of soluble and immobilized biocatalysts, including whole-cell systems, the authors treat the use of non-aqueous reaction systems, applications in organic synthesis, bioreactor design and reaction engineering.

Contents

Preface

- Introduction to enzyme technology- Basics of enzymes as biocatalysts- Enzyme discovery and protein engineering- Enzymes in organic chemistry- Cells designed by metabolic engineering as biocatalysts for multi-enzyme biotransformations- Enzyme production and purification- Application of enzymes in solution: Soluble enzymes and enzyme systems- Immobilization of enzymes (Including Applications)- Immobilization of microorganisms and cells- Characterization of immobilized biocatalysts- Reactors and process technology- Case study: The one-step enzymatic process to produce 7-ACA from cephalosporin C

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Biography

Born in 1941, Klaus Buchholz studied chemistry at the universities of Saarbrücken und Heidelberg, graduating in 1967. In 1969 he received his PhD from the TU Munich, after which he worked as a researcher at Dechema e.V. in Frankfurt/Main until 1982. In 1981 he qualified as a professor at the TU Braunschweig, where he then became department head at the Institute for Agricultural Technology and Sugar Industry. From 1988 onwards he was the provisional Head of the Institute, before becoming Professor for Technology of Carbohydrates at the Institute for Technical Chemistry in 1991. His main research areas include biocatalysts, enzymatic processes for the modification and synthesis of saccharides, environmental biotechnology, flow bed reactors with immobilized biocatalysts, and the synthesis of saccharide polymers.

Volker Kasche, born in 1939, studied chemistry, mathematics, and physics at the University of Uppsala, Sweden, receiving his degree in 1964. This was followed by a year as a NATO research fellow at Brandeis University, USA. He received his doctorate from the University of Uppsala in 1971, and in 1973 became Professor for Physical Biology at the University of Bremen, Germany. He has been Professor for Biotechnology at the TU Hamburg-Harburg, Germany, since 1986, focusing his research on fundamentals of equilibrium and kinetically controlled reactions catalyzed by free and immobilized hydrolases, the production, post-translational processing and purification of penicillin amidases and serine peptidases by affinity chromatography, as well as fundamentals of mass transfer in chromatography and enzyme technology.

Born in 1964, Uwe Bornscheuer studied chemistry at the University of Hanover, Germany, where he graduated in 1990. After receiving his PhD in 1993 from the Institute of Technical Chemistry at the same university, he spent a postdoctoral year at the University of Nagoya, Japan. He then joined the Institute of Technical Biochemistry, University of Stuttgart, Germany, where he qualified as a professor in 1998. He has been Professor for Technical Chemistry & Biotechnology at the University of Greifswald, Germany since 1999. Professor Bornscheuer's main research interest is the application of enzymes in the synthesis of optically active compounds and in lipid modification.