The effect of breed, season of the year and stage of lactation on the response (milk secretion rate) of exotic dairy cows to thrice-daily milking was studied in 20 Friesian and 10 Jersey cows using the technique of half-udder study. The results indicated that there was a positive response to increased milk¬ing frequency in both breeds. The response by the Friesian during the winter and early summer seasons was similar, regardless of stage of lactation. During summer, the response of the Jersey cows at the post¬peak stage of lactation was greater (P < 0.05) than that of the Friesians, but it was lower (P < 0.01) at the early stage. The response during the post-peak stage was always higher than during the early stage. These increases in rate of milk secretion were due to the increase in secretion rate of the morning milking fol-lowed the extra mid-night milking of only the half udder milked thrice daily, suggesting a local regulatory mechanism for controlling milk secretion, that respond rapidly to milk removal.