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Structure of the yeast centrosome (spindle pole body) Improved FRET methodology in living cells Construct a model of the core layers of the SPB by combining FRET data with prior cryo-EM analysis.

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Y C

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YCYYYYYY C

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Structure of the core of the yeast centrosome (spindle pole body) Improved FRET methodology in living cells Construct a model of the core layers of the SPB by combining FRET data with prior cryo-EM analysis.

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Structure of the core of the yeast centrosome (spindle pole body) Improved FRET methodology in living cells Construct a model of the core layers of the SPB by combining FRET data with prior cryo-EM analysis.

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Conclusions Combining the the relative distance constraints provided by FRET analysis of living cells with the cryo EM allowed construction of a detailed model of the lattice structure of the core of the SPB.