Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: Add fresh medium at the time of subculture

Rinse cell sheet 2 times with fresh 0.25% trypsin, remove trypsin and allow the culture to stand for 5 to 10 minutes at 37C. Add fresh medium, aspirate and dispense into new flasks.

Name of Depositor

Genetics Institute, Inc.

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