Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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CRISPR genome editing

Custom DNA & RNA

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Genes & gene fragments

Next generation sequencing

Optional services

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What DsiRNA concentration do I need to use in my experiment to see knockdown?

For an RNAi or antisense experiment, the actual level of target gene knockdown is related to the transfection efficiency. A positive control such as HPRT DsiRNA should be used in each experiment to assess transfection efficiency. In addition, IDT recommends using a dose-response curve of 0.1, 1, and 10 nM to determine maximum response.