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Purify up to 400 grams of compound at 1L/min up to 75% faster than with traditional glass columns. Biotage® Flash 75/150 is a simple and reliable system that contains everything you need for separation scale up.

The UV absorption spectrum of some solvents
overlaps with the product they dissolve, meaning
that fraction collection processes cannot distinguish
between solvent and product. Luckily, there is
technology that solves this problem.Tags: Application Notes

Our carefully selected portfolio of industrial scale products have a proven track record of successful applications and use in scale-up projects. We can support the discovery, development and manufacturing of customer pharmaceutical and biotechnology products, from pre-clinical, phase I, II and III to small scale commercial operations.Tags: Brochures,
English

Flash purification involves a simple liquid chromatography technique
» Method development uses TLC as a way of deciding the parameters for the
separation
» Isocratic separations are easiest to develop, but gradient separations are
more powerful
» Software in the Isolera helps with conversion of an isocratic separation to
a gradient
» It is possible with the Spektra software to run step gradients
» Loading options are dependent on the column type
» SNAP offers the most flexibility
» Care must be taken to choose the best loading option to get good
purificationsTags: Manuals & User Guides

Improvements in solid phase peptide synthesis strategies and
development of resin linkages susceptible to low acid cleavage
conditions has enabled synthesis of long peptides while keeping the
protecting groups intact. This strategy is now used for the
preparation of chemically synthesized proteins, wherein shorter
peptide fragments are ligated together. They are also found in the
synthesis of peptide macrocycles that utilize head-to-tail cyclization strategies. Although linear synthesis of protected peptides is generally straightforward, purification of these compounds using traditional reversed phase methods is quite challenging. Herein we describe the use of normal phase chromatography for purification of fully protected peptides.Tags: Posters

Natural product chemistry deals with discovering the previously unknown in nature. Compounds found in nature are typically found in low quantity and thus extractions are needed to isolate certain compounds classes or at least compounds with similar solubility.Tags: English,
Posters

For most organic and natural product chemists flash
chromatography is a necessary part of their research. As such, many chemists need quick isolation of at least one desired component from a crude mixture in relatively high yield and purity. This need for speed, purity, and yield pits these desires against each other as you can typically optimize on only two of the three goals.
In this poster, we will describe some techniques that help chemists optimize flash purification and maximize speed, yield, and purity.Tags: English,
Posters

Although capable of very high resolution, RP-HPLC is often limited by low column loading capacity, therefore demanding a significant time investment for peptide purification. As an alternative strategy, reversed-phase flash chromatography can also be used to purify synthetic peptides. The larger particle size used in flash column chromatography enables much larger loading capacity, thereby significantly reducing the time required for peptide purification.Tags: Posters

When it comes to polar organic compound purification,
many chemists turn to normal-phase flash
chromatography often utilizing dichloromethane and
methanol as the eluting solvents. While this can work, it
often can be challenging to optimize due to methanol’s
high polarity and protic chemistry.Tags: English,
Posters

As reversed-phase flash chromatography gains traction in
medicinal chemistry labs the need to monitor its cost and
safety are becoming more important. Commonly used
reversed-phase solvents typically include water with an
organic solvent such as methanol or acetonitrile – each
have advantages and disadvantages.Tags: English,
Posters

For those chemists performing organic synthesis, reaction mixture purification by flash column chromatography is an integral and necessary part of the synthesis process. However, flash chromatography consumes large volumes of solvent which either needs to be recycled or disposed.
ACS 2016.Tags: Posters

Reversed-phase chromatography is typically used when you need to separate several milligrams of relatively polar compounds that either are not soluble in normal-phase solvents or are not compatible with bare silica
because they react, stick, or both. If you are currently using reversed-phase at preparative scale, such as flash chromatography, you know the mobile phase limitations – water with either methanol, acetonitrile, or
THF. As with normal-phase flash chromatography, when it comes time to purify you want your crude sample fully solubilized in the weakest possible solvent at the highest possible concentration.
ACS 2016Tags: Posters

DMSO and DMF are suitable injection solvents for reversed-phase flash purification. DMSO shows it can be loaded in larger volumes (up to 0.05 mL/g of C18 media or 3.5% of a column volume) without affecting chromatographic separations or carrying compounds with it.Tags: Posters

Using the Biotage automated workflow, models have shown that the time taken to produce a target molecule can be reduced by up to eighty percent, allowing project delivery timescales to be reduced
accordingly.Tags: Product Notes

Traditional approaches to compound purification involving chromatography utilize large volumes of relatively toxic and expensive solvents, and significantly contribute to the environmental footprint of organizations involved in molecular research. Current directives for greener chemistry have put pressure on organizations to reduce the
environmental impact of their work.Tags: Product Notes

Purify up to 400 grams of compound at 1 L/min with the Biotage Flash 150™ system, up to 2x faster than traditional glass columns. This robust stainless steel system safely operates at 100 psi enabling high flow rates and the use of higher viscosity solvents. A variety of cartridge media provides
chemists with selectivity choices for optimal purification conditions. Simple and reliable, this system contains everything needed to begin your separations.Tags: Product Notes

These rugged systems safely operate at 100 psi enabling fast flow rates and the use of high viscosity solvents. Biotage’s proven, patented radial compression technology ensures near zero “wall effects” and channeling inside each column. This maintains the bed’s stability, rendering cleaner, purer fractions in less time and higher overall product yield.

Routinely operating at a flow rate of 250 mL/min (Flash 75) and 1L/min (Flash 150), these systems allow you to quickly scale-up and complete runs, saving hours or even days of purification time.

Complete Kit

The Biotage® Flash 75/150 system include an easy-to-install radial compression module, a fully integrated air manifold, solvent reservoir, sample injection module (SIM), a start-up kit with all necessary tubing, grounding kit, and a user’s manual. Prepacked cartridges are ordered separately. Flash 150 compression modules are mounted onto robust portable bases (included in the system package), which are fitted with casters for easy mobility.

Patented radial compression

Biotage’s proprietary packing technique ensures that each Flash cartridge is shipped with a tightly packed bed. The application of our proven, patented radial compressiontechnology maintains the bed’s stability during use for cleaner, purer fractions in less time and higher overall product yield. Extra force from radial compression ensures near zero“wall effects” and channeling inside each column.

Highest flow rate

Routinely operating at a flow rate of 250 mL/min, these Flash systems and cartridges allow you to quickly scale-up and complete runs, saving hours – even days – of purification time. In this comparison between a Flash 75L (75 mm x 300 mm) cartridge and a 11 x 20 cm glass column, fractions were collected in 2.5 hours using the glass column, while the Flash 75L cartridge required just 40 minutes. In addition to the 73% reduction in purification time, there were fewer mixed fractions, resulting in greater product purity. Safer than glass columns All Biotage Flash 75 and 150 cartridges are constructed of medium-density polyethylene to resist cracking and splitting and meet the FDA extractables requirement specified in 21 CFR 177.1520. There is no breakable glass, and all of the silica is completely self-contained, eliminating any exposure to silica dust or contaminants.

Compression modules seal up to 100 psi, ensuring leak-free operation even with high flow rates and reversed-phase solvents

Engineered for fast flows

Fully grounded for safety

Available with a variety of media

Biotage KP-Sil — 40–63 µm, 60 Å, silica

Biotage KP-C18-HS — 35–70 µm, 90 Å, C18-bonded silica

Mitsubishi Diaion™ HP20 and HP20SS SDVB resins

Biotage KP-NH — 40–75 µm, 100 Å, amine functionalized

About Biotage

Biotage is a global Life Science company that develops innovative and effective solutions for separation within organic and analytical chemistry, as well as for industrial applications. Biotage is listed on NASDAQ Stockholm.