How It Works…

Your microRNA sequence is embedded in the 3’ UTR of a marker protein mRNA, which is expressed from an inducible miRNA expression vector (pmRi-mCherry or pmRi-ZsGreen1). When cells expressing the Tet-On Advanced transactivator are transfected with the pmRi-mCherry or pmRi-ZsGreen1 vector, high levels of your inducible microRNA and either the mCherry or ZsGreen1 proteins are expressed in response to doxycycline (Dox). Once Dox is added to the cell culture medium, miRNA induction is very rapid and effective.

Red and Green Complete Systems

The complete Mir-X Inducible miRNA Systems provide the pTet-On Advanced Vector for expressing the Dox-responsive transactivator; an inducible miRNA expression vector (either pmRi-mCherry or pmRi-ZsGreen1; also available separately); two linear selectable markers (hygromycin and puromycin) for cotransfecting with the pmRi vectors in order to establish stable cell lines; and tetracycline-free Tet System Approved FBS (serum) for ensuring the full activity of Tet-based expression systems.

Features

Applications

Tetracycline inducible miRNA expression in mammalian cells

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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