TSA or TSB (for the agar and broth, respectively), are perhaps the most useful media for after-sample analysis of marine bacteria, provided an adequate concentration of salt (NaCl) is added. It is necessary to obtain a final concentration of NaCl between 1.5 and 2.5 %, TSA and TSB have already 0.5 % NaCl (so, add 20 g per liter of medium).

Strains distributed from this Collection are grown in a tube with TSA + 2.0 % NaCl covered with sterile mineral oil.