AAVanced™ Concentration Reagent

One-step rAAV particle isolation from packaging cell media
Traditional rAAV production methods requires multiple steps, including
cell lysis and CsCl2 ultra-high-speed density gradient centrifugation,
chromatography, or binding to affinity matrix columns. These are
difficult to setup, time-consuming, and require specialized equipment
for isolation of high-purity rAAV for in vivo experiments. As many
studies have shown that different rAAV serotypes are efficiently
secreted into the culture medium of transfected 293T cells during rAAV
packaging, SBI has developed a novel reagent to facilitate fast,
high-quality rAAV isolation.

Easiest Protocol to Isolate rAAV Particles

SBI has developed an innovative solution called
AAVanced Concentration Reagent – a simple, one step rAAV concentration
reagent for the isolation of rAAV particles from media. AAVanced
Concentration Reagent is based on a proven nanoparticle technology and
is specifically optimized for the precipitation of most AAV serotypes
from culture medium. The rAAV virus produced using AAVanced
Concentration Reagent has been tested successfully for in vitro and in
vivo applications for multiple serotypes of AAV with no observed
cytotoxic effects, which provides direct evidence for the utility of
this reagent for demanding applications using rAAV. SBI’s AAVanced
Concentration Reagent significantly reduces the complexity and time
required for rAAV virus production, allowing researchers to focus on
their research experiments rather than virus production.

AAVanced Concentration Reagent Produces Robust Virions

How does AAVance virus preps compare to tranditonal cell lysate preps?
To test this comparison, recombinant rAAV was packaged with a PGK-GFP
expression shuttle vector. The rAAV particles were isolated either by
the traditional cell lysis freeze-thaw with centifugation method or with
the AAVanced Concentration Reagent from the packaging cell media. Equal
amounts of rAAV were then added to HEK293 cells to test for infectivity
based on GFP expression. Representative images of GFP expressing cells
10 days after infection.

Stratech Scientific
is a distributor of high quality, competitively priced, reliable
products for research laboratories throughout the UK and Europe. Please
contact us to find out which ranges we can supply in your country.