Evolving eukaryotes: an interview with Joel Dacks

Joel Dacks is an Associate Professor and Canada Research Chair in Evolutionary Cell Biology at the University of Alberta, a Scientific Associate at the Natural History Museum (London), and the current President of the International Society for Evolutionary Protistology. His research group studies the evolution and diversity of the eukaryotic membrane-trafficking system, from origins to potential disease therapeutics. In this interview, Joel shares some perspectives on gaining a balanced view of comparative cell biology and the importance of a constructive peer review process.

My lab studies the evolution and diversity of membrane-trafficking organelles. Using genomics and molecular evolutionary techniques, with a focus on microbial eukaryotes (protists), we are examining the conservation in how material is moved around the cell and the ways in which different lineages across eukaryotes vary from the canonical configuration. This information tells us about the diversity of modern membrane trafficking mechanisms and function. It can also be used to understand the evolutionary processes and details of how the endomembrane organelles, and their associated molecular machinery, arose [1]. Currently, we have several projects examining the genomes of parasitic lineages (and their harmless free-living relatives) to understand the evolution of the specialized membrane-trafficking machinery that underpins parasitic mechanisms [2]. We also have a project using transcriptomics and bioinformatics to investigate the gene regulatory networks and cell biology of contractile vacuoles, endolysosomal organelles that regulate osmotic pressure in freshwater protists. Finally, we are delving into the evolution of key membrane-trafficking families such as SNAREs (e.g., [3]), vesicle coats (e.g., [4]), and GTPases (e.g., [5]).

There has been a major community effort to produce tractable molecular cell biological model systems in the areas of the eukaryotic tree other than plants, animals, and fungi (e.g., the MMI program [6]). I expect that these efforts are going to increasingly produce a wealth of comparative data, enabling a more balanced and informed view of all of eukaryotic cell biology. Also, the field of eukaryogenesis was revolutionized in the past few years by the discovery of the Asgard archaea as the closest prokaryotic relatives of eukaryotes [7]. Efforts to isolate and culture these organisms will lead to even greater advances in understanding both a previously unknown type of organism and the contributors to eukaryogenesis. I also hope that new lineages will be described which are even closer to eukaryotes than the Asgard archaea already described.

I think that the peer-review process is more important now than ever. With the vast amount of material available online that can be used to inform and mis-inform, a rigorous peer-review process is absolutely crucial since it is what sets scientific data apart from opinion backed by anecdotal facts. As well, we as a community rely on the good-will and sense of responsibility of our colleagues to keep the peer-review system working. My colleagues do this, and I feel a responsibility to contribute.

My two best peer-review experiences, in retrospect though perhaps not at the time, were both rejections. The first was a rejection of a paper I submitted whilst in graduate school, where the editor told me that the result was interesting but that I needed to collect more data to test my interpretation of the findings that we had. But the next data that we collected were seemingly contradictory to the first! So we kept collecting and looking at different data points to bring into the study. Because we had to keep pushing and piece together data that were more complex than initially anticipated, we ended up coming up with a very different paper, one that laid the foundation for our proposed mechanism of how non-endosymbiotically derived eukaryotic organelles evolve. The second experience was quite recent. We had submitted our work to several high impact journals and received form-letter rejections. Finally, we got a rejection but with detailed and thoughtful criticism. It turned out that the reviewers were interpreting our paper in a way that I had not anticipated, prompting me to rewrite the entire manuscript to clarify our points. The next submission to a different high impact journal was completely straightforward and positive.

Website:

Acknowledgements

JBD wishes to thank members of the Dacks lab, past and present, for their contributions.

Funding

JBD is the Canada Research Chair (Tier II) in Evolutionary Cell Biology. Research in the Dacks lab is supported by a Discovery Grant (RES0021028).

Availability of data and materials

Not applicable.

Author’s contributions

JBD wrote the manuscript. JBD read and approved the final manuscript.

Competing interests

The author declares he has no competing interests.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.