Molecular characterization of Iranian wheat stripe virus shows its taxonomic position as a distinct species in the genus Tenuivirus

Molecular characterization of Iranian wheat stripe virus shows its taxonomic position as a...
Heydarnejad, J.; Barclay, W. S.; Izadpanah, K.; Hunter, F. R.; Gooding, M. J.
2006-02-01 00:00:00
The full lengths of three genome segments of Iranian wheat stripe virus (IWSV) were amplified by reverse transcription (RT) followed by polymerase chain reaction (PCR) using a primer complementary to tenuivirus conserved terminal sequences. The segments were sequenced and found to comprise 3469, 2337, and 1831 nt, respectively. The gene organization of these segments is similar to that of other known tenuiviruses, each displaying an ambisense coding strategy. IWSV segments, however, are different from those of other viruses with respect to the number of nucleotides and deduced amino acid sequence for each ORF. Depending on the segment, the first 16–22 nt at the 5′ end and the first 16 nt at the 3′ end are highly conserved among IWSV and rice hoja blanca virus (RHBV), rice stripe virus (RSV) and maize stripe virus (MStV). In addition, the first 15–18 nt at the 5′ end are complementary to the first 16–18 nt at the 3′ end. Phylogenetic analyses showed close similarity and a common ancestor for IWSV, RHBV, and Echinochloa hoja blanca virus (EHBV). These findings confirm the position of IWSV as a distinct species in the genus Tenuivirus .
http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.pngArchives of VirologySpringer Journalshttp://www.deepdyve.com/lp/springer-journals/molecular-characterization-of-iranian-wheat-stripe-virus-shows-its-3JXlm0PNtc

Molecular characterization of Iranian wheat stripe virus shows its taxonomic position as a distinct species in the genus Tenuivirus

Abstract

The full lengths of three genome segments of Iranian wheat stripe virus (IWSV) were amplified by reverse transcription (RT) followed by polymerase chain reaction (PCR) using a primer complementary to tenuivirus conserved terminal sequences. The segments were sequenced and found to comprise 3469, 2337, and 1831 nt, respectively. The gene organization of these segments is similar to that of other known tenuiviruses, each displaying an ambisense coding strategy. IWSV segments, however, are different from those of other viruses with respect to the number of nucleotides and deduced amino acid sequence for each ORF. Depending on the segment, the first 16–22 nt at the 5′ end and the first 16 nt at the 3′ end are highly conserved among IWSV and rice hoja blanca virus (RHBV), rice stripe virus (RSV) and maize stripe virus (MStV). In addition, the first 15–18 nt at the 5′ end are complementary to the first 16–18 nt at the 3′ end. Phylogenetic analyses showed close similarity and a common ancestor for IWSV, RHBV, and Echinochloa hoja blanca virus (EHBV). These findings confirm the position of IWSV as a distinct species in the genus Tenuivirus .

Journal

Archives of Virology
– Springer Journals

Published: Feb 1, 2006

Recommended Articles

Loading...

References

Sequence analysis of rice hoja blanca tenuivirus RNA 3.

De Miranda, JR; Hernandez, M; Hull, R; Espinoza, AM

Sequence of the PV2 gene of rice hoja blanca tenuivirus RNA-2.

De Miranda, JR; Hull, R; Espinoza, AM

Sequence of Echinochloa hoja blanca tenuivirus RNA-3.

De Miranda, JR; Munoz, M; Madriz, J; Wu, R; Espinoza, AM

Sequence of Echinochloa hoja blanca tenuivirus RNA-4.

De Miranda, JR; Munoz, M; Madriz, J; Wu, R; Espinoza, AM

Sequence of rice hoja blanca tenuivirus RNA-2.

De Miranda, JR; Munoz, M; Madriz, J; Wu, R; Hull, R; Espinoza, AM

Maize stripe tenuivirus RNA2 transcripts in plant and insect hosts and analysis of pvc2, a protein similar to the Phlebovirus virion membrane glycoproteins.