Abstract

This research describes the development and validation of a suitable analytical method, based on GC-MS, to detect nicotine in larvae, pupae, empty puparia and adults of blowfly Calliphora vomitoria L. (Diptera: Calliphoridae). Furthermore, the effects on the blowfly survival and growth rate were examined when reared on substrates spiked with three concentrations of nicotine, sufficient to cause death in humans.