Description:
Autophagy is a catabolic process for the autophagosomic- lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin-E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).

Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.

Storage and Stability:For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type

Isotype

Clone

Grade

Pab

IgG

Affinity Purified

Size

Storage

Shipping

Source

Host

100ul

4°C (-20°C Glycerol)

Blue Ice

Human

Rabbit

Concentration:

Not determined

Immunogen:

Synthetic peptide corresponding to residues near the carboxyl-terminus of Atg5. (KLH)