Usage & Assay Information

The ED50 as determined by a cytopathic affect inhibition assay with Mouse L929 cells challenged with EMC virus was 1 x 10^7 units/mg.

Storage & Safety

Storage

Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Endotoxin Levels

Endotoxin level is less than 0.1 ng per ug (1 EU/ug).

General Information

Biological Significance

IFN-gamma is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-gamma receptors are present in most immune cells, which respond to IFN-gamma signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-gamma signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. Additionally, IFN-gamma stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-gamma is species-specific and is biologically active only in human and primate cells.