To determine the extent to which extracellular matrix proteins colocalize with the microtubule-anchoring factor LL5s (LL5α), MCF-10A epithelial cells were immunostained with antibodies to the secreted autocrine laminin-5 (green), the laminin receptor integrin α3 (blue) and LL5s (red). Analyses suggest that LL5s are colocalized with activated pools of integrins associated with laminin-5. Overall findings from this publication showed that signaling from laminin-integrin associations plays a role in attaching microtubule plus ends to the epithelial basal cell cortex. This image is the original data file for Fig. 3 Panel D-a from J Cell Biol (2010) 189 (5):901-917.