Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] 图像

ab76157 stained HepG2 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76157 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Following up my previous message, I received confirmation that, for the IDO antibody ab76157, no testing has been done on rat samples. I can send this as a replacement but if you would rather have a credit or refund for ab106134, I can issue that inst...

The producer of this clone has provided the following information about the immunogen for ab76157:
"Sequence homology of the immunogen with rat IDO is 86%. Species cross-reactivity data seems to be missing, so I will update you soon."
It is not c...