Rosetta Calibration

Rosetta Calibration is an easy-to-use product that turns your flow cytometer into a particle size analyzer.

Why use Rosetta Calibration?

You want to measure the size of your particles, but flow cytometry measurements are in arbitrary units. Use Rosetta Calibration to convert the arbitrary units of the light scattering signal to nanometers, know what you are measuring, and compare your measurements to other flow cytometers and laboratories.

How does Rosetta Calibration work?

Rosetta Calibration consists of beads and software and works as follows:

you run the beads

you load the bead measurement in the software

the software automatically determines the scatter to diameter relationship for your particles and flow cytometer

Please read the publications for more details about Rosetta Calibration.

Applicability

Rosetta Calibration is developed to standardize flow cytometry measurements on spherical particles <10,000 nm, such as extracellular vesicles and liposomes. One vial of Rosetta Calibration beads contains sufficient material to calibrate your flow cytometer 25 times. We recommend to calibrate your flow cytometer weekly. If you change the settings on the scatter channels, you need to repeat the calibration. We currently support the following flow cytometers:

Apogee A50-Micro

Apogee A60-Micro

Beckman Coulter Astrios

Beckman Coulter CytoFlex

Beckman Coulter Gallios

Beckman Coulter Navios

Becton Dickinson FACSAria

Becton Dickinson FACSAria II

Becton Dickinson FACSCalibur

Becton Dickinson FACSCanto

Becton Dickinson FACSCanto II

Becton Dickinson FACSVerse

Becton Dickinson Influx

Becton Dickinson LSR Fortessa

Becton Dickinson LSR II

Stratedigm S1000

The vial also contains an activation code for the Rosetta Calibration software.

Price

The price of Rosetta Calibration is €399,- in Europe and $399,- elsewhere (excluding VAT and shipping costs). Please ask for a quotation via info@exometry.com.

Demo

Download and install Rosetta Calibration and use "Demo" as a licence key to calibrate our demo files.