Investigator Triplex DSF Kit

For multiplex amplification of the D3S1358, FGA (FIBRA), and SE33 (ACTBP2) loci

Simultaneous amplification of 3 discriminatory markers

Highly suited for degraded DNA samples

Highly sensitive and inhibitor resistant

No overlapping allelic ranges within a panel

The Investigator Triplex DSF Kit is for prescreening of human DNA samples and can be used as a starting point for genotyping strategies in human identity and forensic testing. It enables simultaneous amplification of STR loci D3S1358, SE33 (ACTBP2), and FGA (FIBRA) from low-copy and degraded DNA. The genetic analysis is calibrated with the DS-30 matrix.

The Investigator Triplex DSF Kit is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease.

The Investigator Triplex DSF Kit enables the simultaneous amplification of the 3 polymorphic STR loci D3S1358, SE33 (ACTBP2), and FGA (FIBRA) using two flourescence label dyes. The kit includes optimized primers and reagents.

Procedure

The 3 polymorphic STR loci are amplified simultaneously and labeled with the flourescent dyes as listed in the table "Investigator Triplex DSF dyes and corresponding markers".

The amplified loci undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as QIAGEN's Investigator IDproof or IDproof Mixture Software. See the table "Technical specifications" for more information.

For further information on which version of the v9 template files is suitable for your needs, please refer to the Template File Info Note that can also be found in the ''Template File'' section of the product resources.

For further information on which version of the v9 template files is suitable for your needs, please refer to the Template File Info Note that can also be found in the ''Template File'' section of the product resources.