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Predicted species reactivity based on immunogen sequence: Mouse: (75%)

IMMUNOGEN:

IKK epsilon antibody was raised against a 16 amino acid peptide near the carboxy terminus of human IKK epsilon.

The immunogen is located within the last 50 amino acids of IKK epsilon.

TESTED APPLICATIONS:

ELISA, IF, IHC-P, WB

APPLICATIONS:

IKK epsilon antibody can be used for detection of IKK epsilon by Western blot at 1 μg/mL. An 80 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

IKK epsilon antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Optimal dilutions for each application to be determined by the researcher.

Background and References

BACKGROUND:

IKK epsilon Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. IκB is phosphorylated by IκB kinase (IKK) complex that contains IKKα, IKKβ, and IKKγ. A novel molecule in the IKK complex was recently identified and designated IKKε/IKK-i. IKK epsilon is required for the activation of NF-κB by PMA and T cell receptors but not by TNFα and IL-1. IKKε/IKK-i message is expressed in a variety of tissues and is inducible by TNFα, IL-1, and LPS.