Syringe Guide

The Syringe Guide is a cost effective plunger support device. It is compatible with the 700, 1700 and 7000 series syringes. With only two guide arms the Syringe Guide protects the plunger from damage but should not be used to improve accuracy and precision.

What is the accuracy of Hamilton syringes?
Hamilton syringes are manufactured to be accurate within ±1% of nominal volume, and with precision within 1%, measured at 80% of total scale volume. The design of our barrel and plunger dimensions assures high levels of accuracy and precision. The Hamilton Company Quality System is ISO 9001-2008 certified.

[Back to Top]Can Hamilton syringes be used on humans?
Hamilton syringes are not manufactured for human use. To discuss potential off label uses for Hamilton syringes please Contact Us.

[Back to Top]Is the volume of fluid in the needle part of the total volume of the dispensed fluid?
When using a Hamilton syringe, the volume in the needle (called dead volume) is considered constant and the volume being dispensed is absolute. The same dead volume is maintained throughout aspirate and dispense stages.

[Back to Top]Are the plungers for the 700 series Microliter syringes interchangeable?
No, they are not! Each plunger is fitted to a specific syringe barrel. Be very careful to keep each plunger with its original syringe in order to maximize syringe performance.

What are the most versatile syringes made by Hamilton?
Gastight syringes are the most versatile syringes. Plungers are replaceable if they get bent. Any of the Metal hub, Kel-F or Removable needle allow for the replacement of needle if they become bent or plugged.[Back to Top]How can I sterilize my syringe?

Chemical sterilization is the preferred method for all Hamilton products. Autoclaving syringes should only be used as a last resort and only where specifically stated.

Syringe sterilization is not straightforward. Not all syringes can be autoclaved. Click here for a list of Hamilton products and their preferred sterilization method.

1. What is your application?
Chromatography, Life Science, etc and what instrument are you using?

2. Do you already have a syringe, if so what kind?
What volume and what termination? Cemented, RN, TLL etc

3. What gauge do you think you need?
We make 10 gauge all the way down to 34 gauge.

4. What length do you need?

Our standard length is 2 inches and all other lengths are considered a custom product

Hamilton makes thousands of different needles, which is why we need as much information from the enduser as possible.

[Back to Top]Is the Digital Syringe automated or manual?
The Digital Syringe is a manual syringe with a digital display which allows you to clearly see the aspirated volume. The Digital Syringe is not automated, it will not dispense with the touch of a button. It operates like a typical syringe, but there is no guessing as to the volume being aspirated or dispensed.

The "s" represents a smaller inner diameter for the needle and a thicker needle wall for better durability. For example, a 26 gauge needle has an outer diameter of 0.46 mm and an inner diameter of 0.26 mm while the 26s gauge needle has an outer diameter of 0.47 mm and an inner diameter of 0.13 mm. The 26s has half the inner diameter of the 26 gauge needle. The difference in the wall thickness also nearly doubled with 26s gauge having a thickness of 0.18 mm while the 26 gauge is only 0.10 mm.

[Back to Top]Which rheostat is recommended to work with the syringe cleaner?

The syringe cleaner requires a Variable Autotransformer and we recommended finding the rheostat at an electronics supply house like Newark Electronics. For example: Newark Electronics - P/N 66F3591 or Tenma P/N 72-110

How can I determine the accuracy of my Hamilton syringe?
Hamilton has developed a gravimetric method for determining the accuracy of our syringes. Click here for the procedure.

I keep bending the plungers on my syringes. How can I prevent this?
The plungers, especially for the smaller volumes bend very easily if not aligned properly or if they are depressed at an angle. To combat this very common problem Hamilton has developed several different products:

For the 700 series, reinforced plungers are available in the 600, 800, 900 series syringes.

For the 1700 series, reinforced plungers are available in the 1800 series. (Reinforced plungers are more cost effective for users who often bend plungers.)

Also available are syringe guides designed to maintain proper plunger alignment. As well as reproducibility guides, which serve two purposes:

Help increase the precision of the injections

Guide the plunger and maintain plunger alignment

The last option to save a syringe with a bent plunger is to buy a Gastight syringe. The plungers can be replaced at a fraction of the cost of a new syringe.

The plunger in my syringe is stuck. What can I do to free it up?
Frozen plungers are caused by improper care of your syringe. Here are a couple of suggestions to save your syringe:

Click here for syringe cleaning and maintenance tips.

Soak the syringe in alcohol, acetone or warm water. Do not soak them for longer than 5 minutes.

Sonic cleaners can sometimes help to free up the plunger.

If the plunger does not start to move after this, you will need to replace the syringe.

Proper cleaning will help prevent frozen plungers. Especially when the syringes are being used for life science applications, where sugary or protein based sample are required. We recommend using a like solvent to dissolves the sample. Rinse the syringe thoroughly and then remove the plunger and allow the syringe and plunger to air dry separately.

Which Hamilton syringe do I have?
Hamilton Company manufactures thousands of different syringes and many of those syringes have the same model number. (the model number is listed on the syringe barrel and is a 3 or 4 digit number, 1701, 1001 or 7001) Because we make multiple variations of each model, it is very important to save the original packaging or at least the original part number for when you need to reorder.

Why won't my new removable needles fit into my removable needle syringe?
All removable needles have a white PTFE ferrule and the ferrule must be removed before the replacement needle can be installed. Generally, the ferrule will come out along with the needle but sometimes it remains behind. If the ferrule does not come out, here are a couple of procedures you can try to get the ferrule out:

Push the plunger all the way through, depending on the size the syringe the ferrule should pop right out.

Hold the needle at an angle as you pull out the needle, the end of the needle will help to pull the ferrule out.

Download
Guide to Selecting the Right Hamilton Syringe for Your Application

Download
Guide to Maintaining and Using Hamilton Syringes

Gauge Index

When selecting a needle gauge it is important to keep in mind the volume of the syringe and the dead volume of the needle. For example, it will be very difficult to prime a 10 µL syringe if the dead volume in the needle is greater than 10 µL. Refer to the gauge index to choose a needle gauge with an appropriate µL/inch before selecting a needle. Select the minimum length that allows you to
carry out your application comfortably.

Note: The ‘s’ on a 22s needle represents a smaller I.D. (inner diameter) for the needle and a thicker needle wall for better durability. For example, a 26 gauge needle has an O.D. (outer diameter) of 0.46 mm and an I.D. of 0.26 mm while the 26s gauge needle has an O.D. of 0.47 mm and an I.D. of 0.13 mm. The 26s has half the I.D. of the 26 gauge needle. Also, the difference in the wall thickness nearly doubles with 26s gauge having a thickness of 0.18 mm while the 26 gauge is only 0.10 mm.

Terminations

Terminations are located at the end of the syringe barrel and function as the interface between the syringe and its mating connection such as the needle. Terminations are offered in a number of different needle and connection configurations to accommodate a broad range of applications.

Below is a listing of the most popular syringe terminations.

N, Cemented Needle
For low volume syringes the needles are cemented into the glass syringe barrel at a point corresponding to the zero graduation mark. With this termination, dead volume is limited to the internal volume of the needle. Not autoclavable. Needle gauge is determined by the syringe volume and are not user-selectable.

SN, Special Cemented Needle
For low volume syringes the special needle terminations are the same as the Cemented Needle terminations except they allow for a variety of user-defined gauges, lengths and point styles to be attached.

LTN, Luer Tip Cemented Needle
For mid volume syringes the needles are cemented into the glass syringe barrel at a point corresponding to the zero graduation mark. With this termination, dead volume is limited to the internal volume of the needle. Not autoclavable. Needle gauge is determined by the syringe volume and are not user-selectable.

LTSN, Luer Tip Special Cemented Needle
For mid volume syringes the special needle terminations are the same as the Luer Tip Cemented Needle terminations except they allow for a variety of user-defined gauges, lengths and point styles to be attached.

LT, Luer Tip
The needles are removable and fit over a ground glass hub which is tapered in the shape of a male luer. The LT termination will accept most hypodermic needles but was designed specifically for use with Hamilton Kel-F needles. This termination increases the dead volume in the syringe, which may not be appropriate for some applications. Autoclavable when disassembled.

RN, Removable Needle
The needles are removable and are a Hamilton-specific design. The design allows the needles to seat precisely at the zero graduation mark of the syringe. Users can select the needle gauge, length and point style to optimize the syringe for custom applications. Additionally, this termination allows for a removable needle without increasing the dead volume of the syringe and is ideal when there is a risk of the needle clogging. Autoclavable when disassembled. Repeated autoclaving will shorten syringe life.

KH, Knurled Hub
The knurled hub is used exclusively on 7000 Series syringes. The hub handles up to 6000 psig maximum injection pressure. The needle is removable but with a limited number of gauges available because the plunger is fitted inside the needle. Autoclavable when disassembled. Repeated autoclaving will shorten syringe life.

FN, Fixed Needle
This termination is found on CTC chromatography syringes. The unique design features a direct attachment of the needle to the barrel and eliminates contact between the sample solvent and the adhesive reducing carry-over.

TLL, PTFE® Luer Lock
This termination has a PTFE, male Luer taper with nickel-plated brass locking hub for use with Kel-F needles, metal hub needles and universal connectors. Also, the TLL is used with Hamilton Diluters/Dispensers, OEM applications and manual operations. Autoclavable when disassembled, except on 25 mL and greater syringes. Repeated autoclaving will shorten syringe life.

BFP, Bubble Free Prime
This syringe is used on syringe pumps like the Microlab 600 Diluters/Dispensers conical plunger tip to flush all liquid from this termination. The resulting syringe is quicker to prime and flush during washes or solvent changes.

SL, SampleLock™
The SampleLock incorporates an On/Off syringe valve with RN needle. This termination is used for headspace, environmental sample collection and storage, pre-pressurization of gaseous samples for GC analysis, and sample spiking. Not autoclavable.

C, ChemSeal
The ChemSeal termination features a ¼-28 UNF male fitting. This syringe is used in low volume applications where system dead volume needs to be minimized. These syringes can be screwed directly into Hamilton HV, HVP, HVX valves.

CA, Carbon Analyzer
This syringe is used for water analysis with total organic carbon (TOC) analyzers. The termination is designed to minimize the chance of organic carbon contamination from the needle connection.

DX, Diluter with Stop
This is a PTFE Luer Lock Male fitting with an M6 female side port. These syringes attach to instrumentation such as the Microlab 500 series diluters and dispensers.

DAD, Diluter AccuDil
These syringes have an axial fine thread M8 x 0.75 and an M6 female side port. These syringes attach to instrumentation such as the Microlab 1000 series diluters and dispensers.

AD, AccuDil
These syringes have an axial fine thread M8 x 0.75. These syringes attach to instrumentation such as the Microlab 1000 series Diluters/Dispensers.

Tracheal
This termination accepts 5/8" I.D. flexible tubing. These syringes are used for air sampling, preparing gas standards, calibrating reservoirs and pneumographs.

Plunger Types

Microliter Plunger

This is the standard, stainless steel plunger found in 2.5 – 500 µL volume Hamilton Microliter syringes. This plunger is hand fitted to the glass barrel to a tolerance of millionths of an inch to create a liquid tight seal ideal for dispensing solvents. When using samples without dissolved particles, the Microliter plunger has an almost unlimited lifespan because there are no wear surfaces. Also, these plungers are not interchangeable between syringes or replaceable due to the custom manufacturing process.

7000 Series Microliter Plunger

This plunger is exclusively for 0.5 – 5 µL volume 7000 series syringes. The end of the plunger has a tungsten wire extension that fits the entire length of the needle all the way to the tip, resulting in zero dead volume. This 7000 series syringe is used for dispensing minute liquid volumes.

Gastight Plungers

Hamilton manufactures a variety of Gastight plungers depending on the intended use of the syringe. All Gastight plungers are made of stainless steel or aluminum and feature a polymer plunger tip, often PTFE. These plungers can be used for manual or automated applications with liquids or gases. The plungers are interchangeable and replaceable for 10 µL – 100 mL volume syringes.

Manual Plungers

Manual plungers come in two designs, one features a standard dome button and the other features a threaded plunger button. Syringes smaller than 1 mL have a stainless steel plunger for syringes, as shown in the picture on the left. Manual plungers 1 mL and larger are coated with PTFE and come with a threaded hole that allows them to be mounted into automated syringe pumps, which is demonstrated by the image on the right.

X-Style Plunger

This plunger is used for syringes 500 µL and smaller intended for use in an automated syringe pump. A special plunger button protects the delicate plunger wire by stopping the pump at the zero line before damaging the tip. The stop also provides a 6-32 threaded hole that is used to mount the plunger into the syringe drive.

XP-Style Plunger

This plunger is used on XP-3000 modular digital syringe pumps and has a stop to limit damage to the plunger tip. This plunger is for half-height syringes with a 30 mm stroke length.

XL-Style Plunger

This plunger is used on the XL-3000 modular digital syringe pumps and has a stop to limit damage to the plunger tip. This plunger is for full-height syringes with a 60 mm stroke length.

XB-Style Plunger

This plunger features a stop and a back bushing. The back bushing increases the plunger tip life, maintains proper plunger alignment and reduces the particles generated by plunger and glass contact.

Bubble Free Prime Plunger (BFP)

This plunger is used exclusively in syringes for Microlab 600 diluters and dispensers. The plunger features a conical plunger tip that extends through the threaded termination of the syringe into the Microlab 600 valve. This design helps remove air from the system and reduces the priming cycles required.

Reinforced Plunger

Small volume syringes require extremely small plunger wires. For uses that are prone to bending and breaking these small plungers, it is recommended using a reinforced plunger. These plungers are available in both Microliter and Gastight versions in the 800 and 1800 syringe series respectively.

Needle Point Style Guide

Several different needle point styles are offered on Hamilton syringe and needle products depending on the application. The correct point style varies depending on the intended application. Below are a few examples of common applications.

Manual GC Injections: Historically a Point Style 2 was required to achieve efficient septum penetration with minimized coring. With Hamilton’s new line of GC septa there is evidence that indicates the Point Style AS will consistently extend septa life by as much as 10-fold.

Manual HPLC Injections: Most manual injection valves are designed to be used with a 22 gauge Point Style 3 needle.

Drawing Sample: Any point style will efficiently draw a sample, but when it is important to get the last drop out of a vial use Point Style 3 or AS to reach all the way to the bottom.

Animal Injections: Animal injections require the sharpest needle possible. The recommended needle point style for this application is a Point Style 4 at a 12° bevel. For applications where injection site depth is critical or the target is small, a more vertical bevel as shown below, such as a 30 or 45°, is suggested.

Bevels for the point style 4 needle may vary from 12-60°.

Note: Syringes and needles manufactured by Hamilton Company are intended for scientific research and laboratory use only and are not intended for human in vivo use.

Point Style 3
Blunt needle point for use with HPLC injection valves and for
sample pipetting. Available gauges: 34-10.

Point Style 3T
Blunt needle point for thin layer chromatography (TLC) applications. Needles are coated with PTFE 3/4" up from the needle tip. Available gauges: 26s, 26, 22s, 22.

Point Style 4
Standard 12° beveled needle point is recommended for life science
applications. Available gauges: 34-10. Special point styles such as
30°, 45°, or any other angle are available upon request.

Point Style 5
Conical needle with side port for penetration of septa, thin-gauged
vinyls and plastics without coring. Available gauges: 26-10.

Point Style AS
Special conical style needle point used on autosampler syringes the
non-coring needle point is recommended for septum penetration.Available gauges: 26-10.

Note: The plunger of a 7000 series syringe uses a tungsten wire that travels through the syringe to the tip of the needle. The seal is created by a PTFE ferrule within the knurled hub termination. The I.D. of the needle is honed to fit the tungsten wire. The displacement calculation is based on the O.D. of the plunger wire which is displayed as Theoretical Bore above. The O.D. is the outside diameter of the glass barrel.

700 Series Syringes

Outer Diameter
Nominal

Inner Diameter
Theoretical Bore

Model

Volume
(µL)

mm

Inches

mm

Inches

75

5

6.6

0.260

0.34

0.014

701

10

6.6

0.260

0.49

0.019

702

25

7.7

0.305

0.73

0.029

705

50

7.7

0.305

1.03

0.041

710

100

7.7

0.305

1.40

0.061

725

250

7.7

0.305

2.30

0.091

750

500

7.7

0.305

3.26

0.135

Volumes are based on 60 mm scale length except models 75 and 701 which are based on 54.1 mm scale length. O.D. dimensions are nominal and typical, I.D. dimensions are theoretical.

1700 Series Syringes

Outer Diameter
Nominal

Inner Diameter
Theoretical Bore

Model

Volume
(µL)

mm

Inches

mm

Inches

1701

10

6.6

0.260

0.46

0.018

1702

25

7.7

0.305

0.73

0.029

1705

50

7.7

0.305

1.03

0.041

1710

100

7.7

0.305

1.40

0.057

1725

250

7.7

0.305

2.30

0.091

1750

500

7.7

0.305

3.26

0.128

1000 Series Syringes

Outer Diameter
Nominal

Inner Diameter
Theoretical Bore

Model

Volume
(mL)

mm

Inches

mm

Inches

1001

1.0

9.0

0.355

4.61

0.181

1001.25

1.25

8.6

0.329

5.15

0.203

1002 (thin wall)

2.5

9.7

0.375

7.29

0.287

1002 (thick wall)

2.5

10.3

0.407

7.29

0.287

1005

5.0

13.5

0.530

10.30

0.406

1010

10.0

17.7

0.695

14.57

0.574

1025

25.0

27.1

1.067

23.03

0.907

1050

50.0

36.9

1.452

32.54

1.282

1100

100.0

36.9

1.452

32.54

1.282

Volumes are based on 60 mm scale length except models 1100 which are based on 120 mm scale length. O.D. dimensions are nominal and typical, I.D. dimensions are theoretical.