Transposable elements mapping for Plan B/C. The final charge will be added when the number of transformant is reported

Service X2

US$100@

Molecular mapping for Plan RH/RI CRISPR HDR transformants. The final charge will be added when the number of CRISPR HDR transformant is reported

Service W1

US$30+10@

Genomic PCR confirmation of insertion on landing sites for Plan H or Plan I. The final charge will be added when the number of transformants is reported

Service W2

US$40+20@

Genomic PCR confirmation of insertion on landing sites for Plan J or PhiC31-RMCE. The final charge will be added when the number of transformants is reported. For Plan J, Service W2 includes free Service W1 (see below detailed Plan J & Service W2 descriptions)

Service W3

US$80+60@

For Plan M only. PCR confirmation of the attP sites for the new MiMIC strain, attB construct and construct/strain background. The $80 charge includes 1 strain and 1 construct, additional construct for the same strain costs $60 per construct

Service W4

US$40+20@

Orientation screening for Plan M lines. Must order "Service W3" if you order "Service W4". The final charge will be added when the number of transformants is reported (see below detailed Plan N & Service W4 descriptions)

Fluorescence

US$50

Screen for fluorescent phenotypes (GFP or RFP/DsRed or YFP or CFP) on the adult eyes. $50 per service quantity. This can be combined with the w+ screening or can be the solo screening of the transformation

vermilion+

US$50

Screen for vermilion phenotype. $50 per service quantity

Loss of XX

US$100

Screen for "Loss of Something" phenotype. $100 per service quantity

New strain

US$100

Handling fee of $100 per new strain. You can send us your own strain for injection. Sending it two months prior to the DNA samples recommended

Shipping

-

You need to provide us your FedEx account. We will use your account for shipping(s) and reshipping(s) - customer is responsible for all shipping costs including Dead on Arrival (DOA). See below

Plan A - P-element Basic Service (US$200): This is an injection-only service
and the cost is based on injecting 200 embryos. We obtain DNA sample of the
P-element in water from you, then further mix it with the helper
plasmid (supplied by you or us) and inject them together into w1118 or yw embryos. The injected larvae will be shipped to you and you are responsible for identifying your own transformants and balancing them. You will pay full amount regardless of the number of transformants found in your lab.

There will be no free second injection in ANY situation including shipping mistake by the couriers and/or by the customs.

Plan B - P-element Standard Service (US$250):This service includes injection, generation and identification of transformants (w+ or y+ included as priced; other markers refer to price table). We will inject your DNA sample into 200 embryos, back-cross the survival G0 adults to w1118 or yw, expand G1 transformants by crossing to w1118 or yw again, and ship only the G2 transformants to you. The G2 transformants
will be mailed to you either as adult flies or as larvae.

All injected G0 adults are individually crossed in single vials! We do not take any short-cut!

When using this service, we guarantee at least 5 independent transformants for a reasonably
sized construct (smaller than 13 kb).

In some special situation, if your injected DNA is lethal, we will inform you
at once and perform the injection again (free of charge). If the 2nd injection is lethal again, the charge for the "confirmed"
lethal injections (200x2 embryo-injections) is US$100.

If your injected DNA has no cloning error, is reasonably sized (smaller than 13 kb) and not lethal
but we get no transformant. We will perform the injection again (free of
charge) and discuss with you the possible reason of this situation and try to
fix the problem. However, if somehow the problem remains, the charge for this situation (200x2 embryo-injections plus
crosses for selecting transformants) is usually half of the Plan B charge (i.e. usually US$125. 200% work but only 50%
service fee). For easier billing purpose we will send you the invoice of full service amount after the 1st injection and will issue the refund, if occured, after the unsuccessful 2nd injection, as store credit for future orders. If you request the 3rd injection, you will have to pay full amount of the service.

If your injected DNA is larger than 13 kb and we get less than 5 transformants, we will not offer free injection. You are suggested to
purchase more service "qty" because usually large constructs are difficult to transform.

Plan C - P-element Premium Service (US$350): This is equivalent to "Plan B plus balancing". This service includes injection, identification of transformants and balancing. We
will inject your DNA, pick transformants and balance them with balancer
chromosomes. We use single balancers. This service takes longer time and you
will be charged the full amount regardless of which chromosome(s) the
P-elements are incorporated in.

Similar to the description of Plan B above, we guarantee at least 4 independent transformants for a reasonable sized construct (smaller than 13 kb).

We will take the same measure and charge in the same way for the special situations (i.e. failure transformation) as described in Plan B.

Plan G - PhiC31 Basic Service (US$200): Similar to the above described Plan A. This is an injection-only service and the cost is based on injecting 200 attP embryos. For P[acman] docking sites injections, some of the G0 embryos will be collected from the crosses between your selected docking-site strain and the PhiC31-source strain. For some P[acman] docking site and all FlyC31 landing site inejctions, the G0 embryos will be collected directly because these strains already contain the PhiC31 source. The injected G0 larvae will be shipped to you and you are responsible for identifying your own transformants and remove the PhiC31 source (by phenotype). You will pay full amount regardless of the number of transformants found in your lab.

There will be no free second injection in ANY situation including shipping mistake by the courier and/or by the customs.

Plan H - PhiC31 Standard Service (US$250): Similar to the above described Plan B and the procedures for Plan G. This service includes injection, generation and identification of transformants (w+ or y+ included as priced; other markers refer to price table). We will inject your attB DNA sample into 200 embryos, back-cross SELECTED survival G0 adults to yw, expand G1 transformants by crossing to yw again, and ship only the G2 transformants to you.

The term of "guarantee" is as of plan B but the number of "5 independent transformants" is changed to "1 independent transformant" due to the fact that in most cases you only need one line to perform your experiment. We will send you maximum 5 lines if there are more found in the screening process.

We will take the same measure and charge in the same way for the special situations (i.e. toxic or failure transformation) as described in Plan B for construct smaller than 13kb. For constructs larger than 13kb, we will charge you full amount regardless of the transformation result.

Plan I - PhiC31 Premium Service (US$280): Similar to the above described Plan C. This service includes injection, generation, identification of transformants and balancing. We will balance the transformants in addition to the Plan H service.

The term of "guarantee" is as of Plan H and we will send you maximum 5 balanced transformants if there are more found in the screening process.

We will take the same measure and charge in the same way for the special situations (i.e. toxic or failure transformation) as described in Plan H.

Plan J - PhiC31 Premium Plus Service (US$320): This service is specific to FlyC31 system (its strains and vectors) and other vectors with the right orientation loxP site. This package includes injection, generation, identification of transformants, balancing and eliminating non-necessary sequences. We will provide the stable lines containing your transgene by Cre-mediated excision leaving ONLY one loxP site and one attL site. We will also send you the corresponding Plan I balanced flies. It's recommended to include the "Service W2 for Plan J" to verify the removal procedures.

Please note we do NOT offer the Plan J service to X-chromosome attP lines (e.g. 24480).

The term of "guarantee" is as of Plan H and we will send you maximum 5 stable lines and their corresponding balanced ones if there are more found in the screening process.

We will take the same measure and charge in the same way for the special situations (i.e. failure transformation) as described in Plan H.

Plan H+I Service (US$305): Plan I plus an early/partial shipment of Plan H. i.e. ship Plan H first (this shipment may not contain all lines), then regular Plan I shipment when finish.

Plan K - PhiC31 non-Standard service - PhiC31 one-shoot-3x-Plan H-Service for large BACs of >30 kb only (US$400): Similar idea of 1.5x price of Plan H and we inject 600+ embryos at once, no free re-injection and no store credits if no transformants. You may choose 1, 2 or 3 strains for the 600+ embryos. Free Service W1 (PCR confirmation for up to 6 lines) is included if there is any transformant. Recommended for testing different landing sites for large BACs. If you wish the resulting lines be balanced, the charge is $10/line (please put this request on the remark); if you wish to receive the regular Plan K shipment plus a later balanced lines shipment, the extra charge is $25 + $10/line.

1. New strain ordering-expanding-preparing ($100 per strain)
This includes the direct BDSC ordering fee (non-refundable if you send us your strain). We order the strain from the BDSC, expand and prepare it for injection. It's recommended to order the Plan N-item#4 (Service W3) below to double check the strain before the injection. We keep the strain for 3 months after the completion of each Plan M.
2. DNA Preparation Service Z1 ($30)
This Plan M package includes the free DNA Preparation Service Z1 (non-refundable if you request to use your DNA directly).
3. Non-standard Plan I - Injection-screening-balancing ($570)

screen for "loss of yellow" or "gain of XX" phenotype. We screen up to 8 individual lines.

balance the G1 founder using proper balancer stock

The term of "guarantee" is as of Plan H but the number of "1 independent transformant" is changed to "2 independent transformants" due to the fact that the MiMIC integration event is orientational. We will send you maximum 8 lines even if there are more found in the screening process. For constructs smaller than 13kb, we may offer a free 2nd injection if we do not get 2 or more lines from the 1st injection; we may offer a 3rd injection or $120 store credit if we do not get any transformants from the first two round of injections. For constructs larger than 13kb, we may offer a free 2nd injection if we do not get 2 or more lines from the 1st injection; we will charge you full amount regardless of the result of the first two round of transformations.
In the case of no injection after we prepare the strain, we only charge $100 if we never perform the injection work.
In the case of one strain multiple constructs injection, we may return $75 per additional constructs for duplicated strain charge. Please contact us in this case.

Plan N - MiMIC Injection Service Package #2 (US$695): This package includes the above Plan M and the below 2 items. The original price was $880, discounted to $695.

4. Service W3 - for Plan M, PCR confirmation of the attP sites for the new MiMIC strain, construct and construct/strain background ($80 includes 1 strain and 1 construct, $60 per addition construct). We design the primers, order the oligos, perform genomic DNA extractions and perform all of the PCR works for you. This is very important to verify the strain and the construct before/for the long Plan M progress.

5. Service W4 - Orientation screening for Plan M lines (US$40 + US$20 per additional lines). Must order "Service W3" if you order "Service W4". By default, we only test 4 lines if we get 4 or more lines. i.e. the minimum charge is $40 if we only get one line. e.g. If we get 8 lines we only test 4 lines, i.e. $40+$20+$20+$20=$100.

Plan RG - Genome Engineering Basic Service (US$225): Similar to the above described Plan A. This is an injection-only service and the cost is based on injecting 200-300 embryos. Due to the nature of the Cas9 lines and/or injection mixes, we may inject 250-300 embryos in most of the cases. Please send us your CRISPR injection mix (DNAs, RNAs, Oligos, etc) or give us a very clear instruction on how to make the injection mix. You will pay full amount regardless of the number of CRISPR transformants by NHEJ or HRD found in your lab.

There will be no free second injection in ANY situation including shipping mistake by the couriers and/or by the customs.

Plan RH - Genome Engineering Standard Service (US$275): Similar to the above described Plan B and the injection procedures for Plan RG. This service includes injection, generation and identification of CRISPR HDR transformants with visible marker (w+ or y+ included as priced; other markers refer to price table). We will inject your CRISPR mix into 200-300 embryos, back-cross SELECTED survival G0 adults to yw (or suitable stock), expand G1 transformants by crossing to yw (or suitable stock) again, and ship only the G2 CRISPR HDR transformants to you. The Plan RH service does NOT include the removal of the Cas9-containing chromosome.

The term of "guarantee" is as of plan B but the number of "5 independent transformants" is changed to "2 independent CRISPR HDR transformants", regardless of the size of the gRNA and donor DNA, due to the high variability of the CRISPR designs. We may re-inject (the second injection) for free if there is 0 or 1 transformant from the first injection round; we will not offer further free injection after the second trial.

We will send you maximum 5 lines if there are more found in the screening process.
If the injection may contain a fluorescence selection marker, please email us to discuss the possible screening options.

We are working very hard to offer mismatch(es) screening services and/or DNA sequencing-based screening services in the near future.

Plan RI - Genome Engineering Premium Service (US$305): Similar to the above described Plan C. This service includes injection, generation, identification of transformants (with visible marker) and balancing. We will balance the CRISPR HDR transformants in addition to the Plan RH service. We may directly setup the G0 crosses using balancers in some situations. The Plan RI service also includes the removal of the Cas9-containing chromosome.

The term of "guarantee" is as of Plan RH and we will send you maximum 5 balanced CRISPR HDR transformants if there are more found in the screening process.

We will take the same measures and charge in the same way for the special situations (i.e. toxic or failure transformation) as described in Plan RH.

Plan D - Other Injections Basic Service (US$200): Similar to the above described Plan A. This is an injection-only service and the cost is based on injecting 200 embryos. We obtain DNA, or RNA or any injectable samples from you, then inject them together into 200 embryos. If you prefer to inject to your own strain, please refer to the below "one-time charge: $100 per new strain". Usually PiggyBac, Minos, or RNA injections were categorized here.

The injected larvae will be shipped to you and you are responsible for identifying your own transformants and balancing them (or you are responsible for all of the following experiments). You will pay full amount regardless of the number of transformants found in your lab (or the result of your follow up experiments). There will be no free second injection in ANY situation including shipping mistake by the courier and/or by the customs.

Plan E - Other Injections Standard Service (US$250): Similar to the above described Plan B and the procedures for Plan D. This service includes injection, generation and identification of transformants (w+ or y+ included as priced; other markers refer to price table).

The term of "guarantee" is as of plan B but the number of "5 independent transformants" is changed to "1 independent transformant". We will send you maximum 10 lines if there are more found in the screening process.
We will take the same measure and charge in the same way for the special situations (i.e. toxic or failure transformation) as described in Plan B for DNA construct smaller than 13kb. For DNA constructs larger than 13kb, we will charge you full amount regardless of the transformation result.

Plan F - Other Injections Premium Service (US$350): Similar to the above described Plan C. This service includes injection, generation, identification of transformants and balancing. We will balance the transformants in addition to the Plan E service.

The term of "guarantee" is as of Plan E and we will send you maximum 10 balanced transformants if there are more found in the screening process.
We will take the same measure and charge in the same way for the special situations (i.e. toxic or failure transformation) as described in Plan B for construct smaller than 13kb. For constructs larger than 13kb, we will charge you full amount regardless of the transformation result.

Plan O Service (US$?): If all of the above standard services may not describe your request. This is the one to let the system assign an order number and the details will be discussed via email. Please ignore the initial money amount showing at the order form.

Regular Midiprep. One prep is good for 3x service quantity. We use the Qiagen Midi column.

Z2

US$50

BAC Midiprep for constructs smaller than 30 kb. For P[acman] constructs requires the EPI300 cells and induction before the DNA prep. We use the Qiagen Midi column.

Z3

US$70

BAC Maxiprep for constructs between 30-80 kb. For P[acman] constructs requires the EPI300 cells and induction before the DNA prep. We use the Qiagen Large Construct Kit.

Z4

US$90

BAC Maxiprep for constructs larger than 80 kb. For P[acman] constructs requires electroporation to the EPI300 cells and induction before the DNA prep. We use the Qiagen Large Construct Kit.

We highly recommend you to include the proper plasmid map along with your sample so that we can do a quality control digest after the DNA prep (free of charge).
A systematic survey of our orders indicated that 62.8% of re-injections using our in-house new DNA prep got significant improvements (i.e. 0 trasnformants to > 5 trasnformants; or 1-2 trasnformants to > 5 trasnformants) v.s. using the original DNA. In the study, we test re-injected our new prep as well as the original DNA side-by-side. The other 37.2% may contain plasmids that may never works (i.e. construct problems, etc.), so the new DNA prep improvement percentage must be even higher. However, we should also take the consideration that the old DNA got frozen at least for one month, i.e. freeze-thaw for at least once.

Service X1 - Transposable elements mapping for Plan B/C transformants (US$100 per line): When you order "Plan B+X1" or "Plan C+X1", we may make genomic DNA extracts of the transposable element transforming flies to be digested with proper restriction enzyme, followed by PCR reactions with primers appropriate for the end of the transposable element.

We will test THREE lines per construct by default (you can request to test more, please let us know). Because we cannot predict how many lines may be transformed, we shall charge your account after the service. The service report, containing the PCR fragment sequences and the cytosites, will be sent to you by email.
When you order Plan E or Plan F and need mapping for the transformants, please email us to discuss the possible services.

Service X2 - Molecular mapping for Plan RH/RI CRISPR HDR transformants (US$100 per line): When you order "Plan RH+X2" or "Plan RI+X2", we may make genomic DNA extracts of CRISPR HDR transforming flies, perform PCR reactions with primers appropriate for both end of the HDR insertion.

We will test TWO lines per CRISPR injection by default (you can request to test more, please let us know). Because we cannot predict how many lines may be transformed, we shall charge your account after the service. The service report, containing the PCR fragment sequences and characterizations, will be sent to you by email.

Service W1 - PCR confirmation for PhiC31 Plan H or Plan I transformants (US$30 + US$10 per additional lines): When you order "Plan H+W1" or "Plan I+W1", we will make genomic DNA extracts of the transformants flies for the PCR tests and send you the electrophoresis results.

We will test TWO lines per construct by default (you may request to test more, please let us know).
Because we cannot predict how many lines may be transformed, we shall charge your account after the service. For example, if you order 1 "Plan H+W1" service for gene-A into the 24483 strain, 1 "Plan I+W1" service for gene-A into the 24487 strain and 1 "Plan I+W1" service for gene-B into the 24488 strain and finally we got 5 w+ flies for each service, we will then only perform PCR for the first 2 w+ lines per service, i.e. 2+2+2=6 y-w+ lines, i.e. $30+$10+$10+$10+$10+$10=$80. The PCR results will be emailed to you after the shipment.

Service W2 - PCR confirmation for Plan J or PhiC31-RMCE transformants (US$40 + US$20 per additional lines): When you order "Plan J+W2", we will make genomic DNA extracts of the transformants flies for the PCR tests and send you the electrophoresis results. For Plan J, we also include the free "Service W1" to test the corresponding Plan I lines (i.e. totally 2 sets of PCR tests).

We will test TWO lines per construct by default (you can request to test more, let us know).

Because we cannot predict how many lines will be transformed, we will charge your account after the service.

Shipping and Handling (US$25 handling): We only charge a US$25 flat rate
handling fee per order, no matter how many services are purchased. For
instance, if you purchased three of the "Plan B - Standard", the total will
only be US$775.00. We will use your FedEx account for sending back flies, so please provide the number when you are registering or ordering. It will be US$50/100 EXTRA shipping charge for domestic/international (including Canada) customers if you do not provide us your FedEx number (UPS or other couriers are fine, please email us for details). In some rare cases, if re-ship is required/requested, we will use your FedEx account; if you did not include any courier account, we will charge you the shipping fee again. The customer is responsible for all shipping costs including Dead on Arrival.

Special handling fee for flies other than w1118, yw or listed landing site lines (US$100): We will charge an extra
handling fee of $100 for the first time you send us the flies. After we finish
the project we may keep two bottles for three months for your future orders. If
you send next order after that period you may have to pay the new stock fee again. Or
you may send us an email to discuss the schedule of the new orders. Special strain does not qualify for the "guarantee" mentioned above for all Plans.

Screening for adult fluorescence phenotypes (US$50 per Service Quantity): For Plan B/C/H/I/J/K/M/N/RH/RI/E/F. Screening for adult fluorescent phenotypes (GFP or RFP/DsRed or YFP or CFP), usually on the adult eyes. This can be combined with the w+ screening or can be the solo screening of the transformation. Please note if the fluorescence screening need to be done at the larvae stage is charge is US$100 per Service Quantity.

Screening extra number of transformants (US$30 per 10 transformants): Screen for more than default number of independent lines (10 for Plan B/C/D/F, 5 for Plan H/I/J/RH/RI), $30 per 10 additional lines. e.g. if one Plan B generated 35 lines you will be charged (35-10)/10x$30=$75 on top of the $250 regular Plan B price. If the extra lines needs to be balanced, the charge will be additional $10 per line.

PCR confirmation for plasmids (US$30 + US$10 per additional reaction): Quality control restriction enzyme digestions (QC RE check) for verification of new DNA prep made at BestGene is free. However if you also wish to check the PCR of the new DNA prep please send us your primers and the charge is US$30 (first reaction) + $10/additional reaction.

Sequencing confirmation for plasmids purified by our in-house DNA preps (US$50 + US$20 per additional reaction): Quality control restriction enzyme digestions (QC RE check) for verification of new DNA prep made at BestGene is free. However if your plasmids has potential unstable sequences and you wish to get it sequenced before the injection, we may send out the in-house prepped plasmid for sequencing using your indicated primer(s). This service is non-refundable since it is done by outside providers (actually shipping is the main cost). The charge is US$50 (first reaction) + $20/additional reaction.

Sequencing for gRNA target site / SNP before Plan RG/RH/RI injection (US$100 + US$25 per additional strain): If you wish to check SNP(s) at gRNA target site of the injection strain before ordering Plan RG/RH/RI, we may make genomic DNA extract of the selected strain, perform PCR reaction with primers appropriate for sequencing the gRNA target site. The raw data ONLY, containing the target site sequencing traces of the PCR fragment, will be sent to you by email.

Stock-keeping fee (US$10 per vial per month): We keep backups for 2-4 weeks after the shipping date. If you request to keep any stock beyond this period the extended stock-keeping fee may apply. The minimum charge unit is 1 vial/month (month = calendar month, e.g. 3/4 - 4/3 = one month, 2/27 - 3/27 = two months).

Returned check charge or re-bill fee (US$30): US$30 will be charged for extra bank/courier administration fee if we got returned check, less wire amount or invalid courier account related to your payment/shipment.

Other services fees: There are no hidden fees! All fees are listed above. However we will inform you in advance if there is any additional works needed to be performed.

Current Promotions:

Pre-pay orders (10% off): For all customers charge their account prior to their ordering. Providing a PO number does not qualify this discount. This discount needs the fund in our account before the actual ordering. There is a minimum of US$2,000.00 per prepay deposit transaction.

Immediate payment orders (2% off): For
all orders (online or offline) submitted together with credit card information. Payment has to be received along with your order form and DNA
sample(s).