The introduction features a history on the Metroid series, the Chozo, the Space Pirates and then goes on to page 6, which has acknowledgements from the authors. It is interesting that the page with the introduction has artwork of the Fusion Suit from Metroid Fusion on it. This may simply refer to the fact that the Fusion Suit is unlockable in the game.

From then on it continues into the walkthrough. Summaries of boss battles are in black sections and the end of the guide features complete maps (the guide has parts of the maps in the walkthrough). Oddly, the guide refers to the Metroid Prime core essence as female.

The end of the guide has supplemental information, this including maps, and various appendices showing information on expansions and Logbook data. Appendix Alpha/Beta shows a chalk-drawing of Samus and carries the expansion information. Appendix Gamma features every Pirate Data entry with a holographic image of Samus. Appendix Delta features a stained-glass window-like design of Samus with each Chozo Lore entry. Finally, Appendix Epsilon has each Research entry. It then goes on into an enemy listing (the cover of which features the Ripper amongst other enemies). The final feature of the guide is a page with information on the secrets ingame, complete with a small, text walkthrough of Metroid, included as a bonus in the game.