Application: The purified BAC DNA contains very low levels of endotoxins and is ready for immediate use in Automated Sequencing (Figure 1), PCR, Restriction Enzyme Digestion (Figure 2), Cloning and other common applications.

Data provided by the Genome Sequencing Center at Washington University, St. Louis

Fig. 1. Click to enlarge.

Purified BAC DNA is suitable for restriction digestionFigure 2. Restriction digestion of BAC DNA isolated with the PhasePrep BAC DNA Kit and two other suppliers (C and Q). BAC DNA samples were purified from overnight cultures of E. coli HB101b transformed with a pbelloBAC11 clone. Approximately 1 µg of DNA from each sample was digested with 20 units of Eco R V at 37°C for 4 hours. One half of each digestion (approximately 0.5 micrograms) was separated by overnight electrophoresis in a 1% agarose gel. Lanes 1 to 8 were BAC DNA purified with Sigma kit, lane 9 was BAC DNA purified with Supplier C's kit, and lane 10 was BAC DNA purified with Supplier Q's kit. The molecular marker used at the first and last lanes was a 1kb DNA Ladder (M) (Product No. D0428).