Antigen
Human Acrp30 (1-244 aa) was expressed in mouse myeloma cells (NSO) and purified (>90%). Recombinant mature protein (19-244 aa, ~25.4 kda) migrates at ~36 kda due to glycosylation. For western blot +ve control (Cat # ACRP302-C), it is supplied in SDS-PAGE sample buffer (reduced). This preparation is not biologically active. It is not suitable for ELISA or other applications where native protein is required. It is supplied in 100 ul/vial. For WB, heat once and load 10 ul/lane and visualize with appropriate antibodies. If the product has been stored for several weeks, then it is recommended to 5 ul of fresh 2x sample buffer per 10 ul of protein control solution prior to heating and loading on gels. This preparation is intended for qualitative purpose and not to serve as standard of known concentration. Store frozen in suitable aliquots. Do not freeze, thaw, or hear repeatedly.