expression problem - expression (Mar/31/2008 )

hi all can any body explain how to purify protien from the inclussion bodies in soluble form (active) i expressed my protien in BL21 (DC3)in pET28a+ vector. but it goes to inclussion bodies and i need it in soluble form

-tkr-

See the QIAexpressionist (attachment). Some suggestions for how to solve this. Especially p. 53.