The OWS is a unique multiphoton system with concurrent, independent multiphoton imaging and experimental laser microbeam capabilities. The primary laser path of the OWS uses a *Spectra-Physics Mai Tai® DeepSee laser tunable between 750-900 nm pumped by a 15W CW 532 nm Millennia laser. A second excitation path contains a Spectra-Physics LSI VSL 337ND nitrogen laser for optical manipulation experiments such as trapping or ablation as described in Review of Scientific Instruments. In addition to the excitation sources, there are two detectors on the OWS. The fluorescence detector is a Hamamatsu H7422 capable of both intensity and lifetime measurements. Additionally, a BioRad MRC-1024 photodiode transmission detector from the BioRad MRC-1024 confocal allows for pseudo brightfield imaging. *Spectra-Physics MaiTai DeepSee manual link is to MaiTai HP, which is same system without pulse compensation.