Article Figures & Data

Figures

Disruption of Swi6 and Dfp1 interaction affects timing of reassociation of centromere to SPBs. We examined CFP-Cnp1 to mark the clustered centromeres, and Sad1-DsRed to mark the SPB, in live cells during mitosis in (A) wt (FY7554) and (B) dfp1-3A (FY7640). Time 0’ corresponds to the timepoint that precedes the first observed separation of the SPB and defines the initiation of mitosis. (C) Quantification of timing when CFP-Cnp1 locates back to the SPBs following mitosis. Significance was calculated with a two-tailed Student’s t-test with * P < 0.05. Bar, 5 µm. CFP, cyan fluorescent protein; SPB, spindle pole bodies; WT, wild-type.

Swi6 and Chp2 are required for the noncentromeric location of Chp1 in interphase. (A) Localization of Chp1-GFP and Swi6-chRFP was observed during mitosis in live WT cells (FY7973). Arrows indicate the position of Swi6 localized close to spindle poles. (B) Live-cell imaging of Swi6-GFP in relation to Sad1-DsRed was captured during mitosis in chp1∆ (FY7978). (C) Localization of Chp1-GFP and Sad1-DsRed in swi6∆ (FY7979). (D) Localization of Chp1-GFP and Sad1-DsRed in chp2∆ (FY8795). (E) Localization of Chp1-GFP and Sad1-DsRed in chp2∆ swi6∆ (FY8726). Time 0’ corresponds to the timepoint that precedes the first observed separation of the SPB and defines the initiation of mitosis. (F) Chp1-GFP signals at centromere and noncentromere regions before mitosis were measured in (A and C–E), and the ratio of Chp1-GFP signals at centromere vs. noncentromere sites is presented. Bar, 5 µm. A two-tailed Student’s t-test was used to determine significance with * P < 0.05, *** P < 0.001. chRFP, mCherry red fluorescent protein; WT, wild-type.

The Genetics Society of America (GSA), founded in 1931, is the professional membership organization for scientific researchers and educators in the field of genetics. Our members work to advance knowledge in the basic mechanisms of inheritance, from the molecular to the population level.