2,3,4,6-Tetra-O-acetyl-β-D-glucopyranosyl thiourea compounds were synthesised from the reaction of aniline and 2,3,4,6-tetra-O-acetylβ-D-glucopyranosyl isothiocyanate in acetonitrile under reflux.The(2,3,4,6-)tetra-O-acetyl-β-D-glucopyranosyl isothiocyanate was synthesised from 2,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl bromine.The structures of the title compounds were confirmed by elemental analyses,IR,MS and()~1H NMR spectra.