mRNA was prepared from root and hypocotyl tissues of the cotton cultivar DeltaEMERALD. cDNA was synthesised from a NotI-oligodT primer/adapter using the manufacturers protocols (Life Technologies) and then ligated to a SalI adapter to facilitate directional cloning. The cDNA was cloned into the SalI and NotI sites of the Lambda ZIPLOX phage vector (Life Technologies). Constructed by Caitriona Dowd and Helen McFadden.