Don't think so... On my 306 I have Filter > Heater > Diffuser, just because I figured the gas would be cooler (being compressed) and may cool water a little before passing over the thermometer in the ETH Heater...

I would think that at the rate which the heater heats the water and the rate at which water moves through the heater (really quite fast), that any effect the heat has on anything is probably pretty negligible. My heater is last because it physically fit easier that way under my tank. I get zero bubbles in my tank with my Cerges reactor.

I have my Co2 reactor first on my outflow pipe then the heater. The reason I did that is so the bubbles from the reactor have the most amount of time in the tubes before hitting the aquarium, I don't know if it helps or not but just seemed to make sense to me that the longer they are in the outflow tube the more delusion i am getting...

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