The simplest method to clear a steroid solution of microorganisms is to heat sterilize it. The typical way to accomplish this is to place the steroid vial in an oven for 30 minutes (bake at 120°C/250°F). You should leave a needle sticking through the rubber top to equalize the pressure inside the vial, or it may break. Note that a higher temperature and duration are typically used for complete dry heat sterilization, but this is not practical for our purposes due to the potential degradation of vial components, and possibly even the drug components. Still, the recommended 30 minutes at 120°C/250°F should kill most bacteria, and can be regarded as a viable harm reduction strategy.

Some people prefer to use the top of the stove for heat sterilization, and submerge their anabolic steroid vials in a pot of boiling water (100°C/212°F). The vial is kept in the boiling water for approximately 20-30 minutes. While this temperature is sufficient for killing many forms of bacteria and other microorganisms, it is generally not considered the best method of sterilization. A higher temperature should be used. It can also be difficult to control the temperature if the vial rests on the bottom of the pot, which is in direct contact with the burner or heat source. Given these problems, it is preferred to attempt heat sterilization in an oven, as discussed above.

Note that trenbolone appears to break down at fairly low heating temperatures.374 Its degradation increases with temperature and duration. Some degradation may be notable even at 100°C/212°F. As such, trenbolone products should be heat sterilized under slightly different conditions than those recommended above. This typically involves baking the vial in an oven for two hours at 80°C/175°F. Again, an unattached needle should be placed in the rubber stopper to equalize pressure.