Center for Applied Genomics (CAG)

The Center for Applied Genomics (CAG) is a genome center in the Research Institute at UCSF Benioff Children's Hospital Oakland. As a pediatric center devoted to research in biology and medicine we are using functional genomic approaches to further the prevention and prediction of the evolution of disease and response to therapy. Our aim is to discover the genetic causes of prevalent childhood diseases and translate findings into medical innovations.

Next Generation Sequencing Services

The Center for Applied Genomics focuses on the genetic and genomic research with an emphasis on next-generation sequencing (NGS). Work at the Center for Applied Genomics ranges from basic research to diagnostics, drug discovery and applications to public health.

Requests

Index of Services

Our facility offers state-of-the-art, Next-Gen services utilizing the 454 GS-FLX+ Titanium™, sequencing platform, which can be used in support of your research projects. We specialize in sequencing genes from pooled amplicons of immunogenetic complexes, including the human leukocyte antigen (HLA), and genes involved in cardiomyopathies. By utilizing amplicon-based Next-Gen technologies, we can provide provide fast and economical genomic research sequence support for immunogenomics, disease association, pharmacogenetics, population genetics and personalized medicine studies.

Our amplicon sequencing is based on ultra deep sequencing of PCR products, and can be used for analyzing genetic variations within a population. The long read lengths and data accuracy of Roche GS FLX+ makes the technology the gold standard for amplicon sequencing. As each molecule within a pool of amplicons can be sequenced individually, the technology has the power to detect rare variants with detection limits of at least 0.5 percent.