Available hosts

Available applications

Background of POLR2J2 antibody

This gene is a member of the R polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, D directed R polymerase II polypeptide J, has been shown to encode a subunit of R polymerase II, the polymerase responsible for synthesizing messenger R in eukaryotes. This locus produces multiple, altertively spliced transcripts that potentially express isoforms with distinct C-termini compared to D directed R polymerase II polypeptide J. Most or all variants are spliced to include additiol non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo. [provided by RefSeq].

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2J2 (RC224755, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2J2.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2J2 (RC224755, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2J2.

HEK293T cells transfected with either RC224755 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-POLR2J2 antibody(TA502454), and then analyzed by flow cytometry.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2J2 (RC224755, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2J2.

HEK293T cells transfected with either RC224755 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-POLR2J2 antibody(TA502455), and then analyzed by flow cytometry.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2J2 (RC224755, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2J2.