Gradual accumulation of somatic mutations in adult stem cells is believed to contribute to cancer development. By combining genome-wide sequencing and stem cell culture technologies, we aim to characterize tissue-specific mutation accumulation in adult stem cells during life and related to disease development. The ultimate goal of these studies is to obtain novel fundamental insight into the processes that shape mutational landscapes in stem cells, a critical step towards understanding cancer aetiology and the development of future therapeutic efforts aimed at prevention.