Abstract

We have used a chromatin immunoprecipitation-microarray (ChIP-array) approach to investigate
the in vivo targets of heat-shock factor (Hsf) in Drosophila embryos. We show that this method identifies Hsf target sites with high fidelity and
resolution. Using cDNA arrays in a genomic search for Hsf targets, we identified 141
genes with highly significant ChIP enrichment. This study firmly establishes the potential
of ChIP-array for whole-genome transcription factor target mapping in vivo using intact whole organisms.