This is a hypotriploid human cell line. Both 61 and 62 chromosome numbers had the highest rate of occurrence in 30 metaphase counts.The rate of higher ploidies was 3%. The t(11q12q), del(11)(q23), 16q+, del(9)(p11), del(1)(p32) and 6 other marker chromosomes were found in most cells. The N13 was usually absent. The Y chromosome is abnormal through translocation to an unidentified chromosomal segment. The X chromosome was present in single copy.

The line is not detectably hormone sensitive, is only weakly positive for acid phosphatase and isolated cells form colonies in soft agar. The cells do not express prostate antigen. Ultrastructural analyses of both the cell line and original tumor revealed microvilli, tonofilaments, desmosomes, any mitochondria, well developed Golgi and heterogenous lysosomes.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

DU 145 was isolated by K.R. Stone et al from a lesion in the brain of a patient with metastatic carcinoma of the prostate and a 3 year history of lymphocytic leukemia.

Permits

These permits may be required for shipping this product:

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.