The Hospital for Sick Children Centre for Applied Genomics

General Information

The Centre for Applied Genomics (TCAG) is dedicated to conducting and promoting research in genomics including service and training support for academic, government, and private sector scientists worldwide.

DNA Sequencing Instruments Used

Applied Biosystems 3730xl

Illumina HiSeq2500

Illumina HiScan-SQ

Ion Torrent Proton

Roche 454 GS FLX Titanium

Life Technologies SOLiD 5500xl

DNA Sequencing Prices (CDN)

$3.50 per successful read

$12.00 / reaction for BAC, LAMBDA, Cosmid and Fosmid

DNA Template Requirements

Plasmids

200-300ng in 7µl

PCR product

> 4kb | 150-200ng in 7µl

2-4 kb | 100-150ng in 7µl

1-2 kb | 50-100ng in 7µl

< 1kb | 50ng in 7µl

< 500bp | 20ng in 7µl

< 200bp | 10ng in 7µl

BAC/Cosmid/Phage DNA

600-800 ng in 12 µl

Primers

5 pmols

Other information

Do not submit Genomic DNA. Failed reads will be charged provided the control from the same sequencing run passes their QC metrics.

Sequencing turn-around time

48 hours

International orders

Yes

GLP DNA sequencing

Not mentioned

Other DNA sequencing related services offered

Next Generation Sequencing

Library Construction

Genotyping

Micro Array Analysis

Oligo Synthesis

Bioinformatics

Contact Information

Jo-Anne Herbrick, Facility Manager

The Centre for Applied Genomics

The Hospital for Sick Children

Peter Gilgan Centre for Research and Learning

686 Bay Street, Room 139800

Toronto, Ontario, M5G 0A4,

Canada

Phone: +1 416-813-8140

Fax: +1 416-813-8319

Email: jherbrick@sickkids.ca

Website: http://www.tcag.ca/index.html

Comments

The centre provides wide range of services with state-of-the-art equipment facility. Typical read length for Sanger sequencing is 700 bases. The center currently averages about 3,500 sequence reads per week.