AIM: The aim of this study was to examine the connection between working in shifts and the sleep quality among medical technicians and nurses, to examine working in shifts with the quality of life and establish a connection between sleep quality, quality of life and working in shifts among medical technicians and nurses in Medical Centre Osijek. PARTICIPANTS AND METHODS: The study was conducted on 188 participants. They were nurses and technicians from Medical Centre Osijek. The following methods were used: Pittsburgh Sleep Quality Index, PSQI, World Health Organization Quality of Life, WHOQOL-BREF and Epworth Sleepiness Scale, ESS. RESULTS: Participants working the first shift experience significantly better and more efficient sleep (P=0,019 P=0,001). Participants, who only work the first shift, wake up considerably earlier (P<0,001). The scale of life quality is slightly higher among the participants who only work the first shift, but there is no statistical importance. Sleep quality is considerably connected to overall quality of life (ρ=-0,488 P<0,001), with all scale dimensions, especially with psychological health (ρ=-0,451 P<0,001). CONCLUSION: Medical technicians and nurses working in shifts have lower sleeping efficiency. Participants working the first shift are more of a morning person and they wake up earlier. Difficulties with staying awake among participants working in shifts affect their mood and optimism. There is a significant connection between working in shifts and sleep and life quality. There are no significant differences regarding working in shifts and quality of life. Medical technicians and nurses working in shifts have somewhat lower values, and elderly participants in both groups have a lower grade of psychological health. Quality of sleep is significantly connected to overall quality of life, mostly with psychological health.