Conditional Knock Out

C57BL/6 Background

The targeting vector has been generated using C57BL/6J DNA and transfected into C57BL/6NTac ES cell line.

Hdac6 exon 2 contains the translation initiation codon.

Exons 11, 12, 13, 14 have been flanked by loxP sites.

The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.

The conditional KO line has been subsequently backcrossed to C57BL/6NTac animals for at least 2 generations and the Flp-transgene was removed by segregation.

The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exons 11, 12, 13, 14 should result in loss of function of the Hdac6 gene by generating a frameshift (premature STOP codon in exon 16).

The remaining recombination sites are located in intron regions of the genome.