Cell Signaling Pathway Profiling Systems

The Pathway Profiling Systems provide a means to assess cell signaling pathway activation in vivo. These systems consist of sets of vectors that each contain a distinct cis-acting enhancer element upstream of a reporter gene. A single transfection experiment provides a profile of the transcription factors that are affected by a given stimulus, drug candidate, or gene product of interest.

Profile Multiple Signal Transduction Pathways

Most of the cis-acting enhancer elements included in our transcription factor systems are regulated by more than one transcription factor, so multiple signal transduction pathways can be monitored. Reporter expression indicates that a transactivator present in the cell has bound the enhancer element in one of the TransFactor Vectors, indicating that a particular signaling pathway or pathways has been activated.

Use the Pathway Profiling SEAP System for broad-spectrum profiling.

Use the Pathway Profiling Luciferase System 4 to study checkpoints in the cell cycle.

Two Reporters Available—Luciferase & SEAP

Luciferase induction can be measured from cell lysates using a standard assay.

Features

Applications

Study activation of cis-acting enhancer elements in vivo

Measure transcription factor activation

Quantify differences in activation

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.