This course gives to the future bioinformaticians the bases necessary to understand the principles of determination of 3D protein structure by X-ray crystallography and nuclear magnetic resonance in order to enable them to make an objective use of the models. It gives them an outline of the capacities and limits of various techniques.

Prerequisite knowledge and skills :

Course of physics of 1st cycle. Basic knowledge in X-ray crystallography. Good knowledge of the basic principles of the structures of proteins. Biophysical course.

Planned learning activities and teaching methods :

Tutorials dedicated to the Pymol software use: obseravtion and analysis of macromolecules structures. Use of PDB database.
Solving of a protein structure in silico on the basis of X-ray diffraction data.

Mode of delivery (face-to-face ; distance-learning) :

To be decided in concertation with the different lecturers.

Recommended or required readings :

The PowerPoint presentations of the theoretical course will be available on CD as well as the notes of practical works.
Books recommended:
- "Crystallography made hook clear: With guide for users for macromolecular models."
Scale Rhodos, Academic Press 2000
- "Introduction to Protein Structure" Carl Branden and John Tooze, Garland Publishing 1999
CD roms:
- PS ² and Kinemage Supplement to "Introduction to Protein Structure" Carl Branden and
John Tooze, Garland Publishing 1999

Assessment methods and criteria :

To be decided in concertation with the different lecturers. Most probably on the basis of a research article.