... In this study, a cDNA of ICE1 (inducer of CBF expression 1) gene, named BcICE1, was isolated from Brassica campestris ‘Longyou 6’. The deduced protein has 499 amino acids containing a typical bHLH domain and is highly identical with AtICE1 (85.9%) from Arabidopsis thaliana. BcICE1 is located in the nucleus. The activities of SOD, CAT, POD, and APX and the transcriptional levels of SOD, CAT, and PO ...

... Asian soybean rust (ASR), caused by the obligate biotrophic fungus Phakopsora pachyrhizi, is responsible for severe yield losses of up to 90% in all soybean producing countries. Till today, eight resistance to Phakopsora pachyrhizi (Rpp) loci have been mapped in soybean. Their resistance mechanism is race specific but largely unknown. The transcriptomes of susceptible BRS184 and Rpp3 with ASR isol ...

... Seedling establishment in an extreme environment requires an integrated genomic and physiological response to survive multiple abiotic stresses. The extremophyte, Haloxylon ammodendron is a pioneer species capable of colonizing temperate desert sand dunes. We investigated the induced and basal transcriptomes in H. ammodendron under water-deficit stress during early seedling establishment. We find ...

... During groundwater recharge with reclaimed water, pentabromodiphenyl ether (BDE-99) might contaminate the underground aquifers. In order to understand the effects of elevated sulfate concentrations on the biodegradation of BDE-99, lab-scale column experiments were performed to study the changes in bacterial and archaeal communities. The results confirmed that the biodegradation rate of BDE-99 impr ...

... Bioreactors have an important role in tissue engineering to improve cell seeding and cell culturing conditions for the production of clinically relevant bone grafts. In our study, we systematically investigated the effects of static and dynamic approaches on cell seeding and in vitro culturing of a scaffold-containing perfusion bioreactor system. For this purpose, MC3T3-E1 pre-osteoblasts were see ...

... The discovery of denitrifying anaerobic methane oxidation (DAMO) has not only improved our understanding of global methane and nitrogen cycles, but also provided new technology options for removal of nitrate from nitrate-contaminated water. Previous studies have demonstrated DAMO organisms could remove nitrate and nitrite from wastewater under strictly anaerobically conditions. In the study, we in ...

... RNA-Seq is becoming the standard technology for large-scale gene expression level measurements, as it offers a number of advantages over microarrays. Standards for RNA-Seq data analysis are, however, in its infancy when compared to those of microarrays. Clustering, which is essential for understanding gene expression data, has been widely investigated w.r.t. microarrays. In what concerns the clust ...

... Biological processes often manifest themselves as coordinated changes across modules, i.e., sets of interacting genes. Commonly, the high dimensionality of genome-scale data prevents the visual identification of such modules, and straightforward computational search through a set of known pathways is a limited approach. Therefore, tools for the data-driven, computational, identification of modules ...