Galactosemia

Overview

Quantase™ Neonatal TGAL Assay

Not subject to the interferences commonly observed with fluorescent and bacterial inhibition methods

Simplified protocols can be used on most plate readers

Rapid results in under 2 hours

Product Highlights

Cost effective

Easily automated

Environmentally friendly, no flammables

Rapid procedure

Flexibility for Your Laboratory Needs

Total Galactose

Punch sample

Add 5 µL elution reagent

Incubate at RT for 30 minutes

Vacuum transfer eluate

Add 100 µL enzyme reagent

Incubate at RT for 30 minutes

Add 100 µL color reagent

Incubate at RT for 2 minutes

Read 570 nm and 690 nm

Clinical Background

Galactosemia is an inherited disorder of galactose metabolism, caused by a deficiency in one of three enzymes, the most important being galactose-1-phosphate uridyl transferase. Galactosemia may also be caused by a lack of galactokinase or UDP-galactose-4-epimerase activity.

Long-term consequences of galactosemia include mental retardation, developmental abnormalities, and even death. The worldwide prevalence of galactosemia is estimated to be 1:60,000–1:80,000. The Quantase Neonatal Total Galactose Screening Assay is an enzymatic colorimetric end-point method for the determination of total D(+) galactose in dried-blood spot specimens.

Bio-Rad's Microplate Neonatal Total Galactose Assay (TGAL) is an enzymatic, colorimetric end-point method for the determination of total D(+)galactose [D(+)galactose + galactose-1-phosphate] in dried blood spot specimens. Recognized as a worldwide market leader in TGAL screening, this assay is easily automated and can be performed with any microplate reader.

What is the education discount policy?

For more than 15 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows high school and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices.

How do I apply for the education discount?

If you are an educator at the high school or college level, visit our Education Discount Policy page to establish an education account number.