Immunoprecipitations of GFP from human cells expressing GFP

Input (I), non-bound (FT) and bound (B) fractions were separated by SDS-PAGE followed by Coomassie staining and Western Blotting. The bound fraction of the conventional GFP antibodies shows in addition to GFP contaminating heavy chain and light chain. The bound fraction of the GFP-Trap® does not contain these contaminating polypeptide chains. Note effective binding of GFP-fusion proteins: No GFP-fusions left in non-bound (FT) lane in Western Blot.