MDRO Susceptibility Testing by Broth Microdilution

The need for clinicians to turn to last-resort antibiotics, such as colistin, to tackle multi-drug resistant organisms (MDRO) is increasing. Accurate antimicrobial susceptibility testing (AST) is essential to support careful antibiotic stewardship to ensure the right antibiotic is prescribed at the right dose for the right duration in order to deliver the best patient outcomes, and to preserve effectiveness.

Having confidence in your AST method and access to test the latest drug combinations is critical but can be a challenge. Obtaining a true MIC by broth microdilution offers a reliable solution.

EUCAST recommends broth microdilution as the only valid method for colistin

Recognising that antimicrobial susceptibility testing of colistin has been ‘fraught with difficulties’ a joint EUCAST and CLSI subcommittee has recommended that broth microdilution (BMD) is so far the only valid method.1EUCAST has also issued warnings regarding some AST methods for piperacillin-tazobactam, antibiotics that are potential alternative treatments for MDROs.

Utilizing the Sensititre system to face the challenge of Gram negative MDRO susceptibility testing

Dr Decousser, from the microbiology laboratory of Henri Mondor Hospital, France, explains how implementing the Thermo Scientific™ Sensititre™ broth microdilution system achieved time savings in reporting results, as well as providing clinicians with accurate and extensive evaluation of minimum inhibitory concentrations (MICs) and alternative therapeutic options.

“We are so pleased with our Gram negative plate that we may now consider a plate for Gram positive organisms," concludes Dr. Decousser.

Thermo Fisher Scientific provided a custom-designed broth microdilution plate with the exact combination and dilution of antibiotics required by the laboratory, in a timely manner.

Through Thermo Fisher Scientific's collaboration with many pharmaceutical companies, the Sensititre system gives you access to the latest antimicrobials to design a Sensititre custom plate tailored to your formulary, dilution ranges and patient population. With the ability to update formats conveniently, you get relevant results that are based on real-time resistance trends and local epidemiology, allowing you to improve clinical success and effectively manage resistance in your institution.

Access the latest antimicrobials, including Ceftazidime/Avibactam and Ceftolozane/Tazobactam, and learn more about our custom-designed Sensititre plates.