Specificity / Sensitivity

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp61 of human CISD1/mitoNEET protein.

Western Blotting

Western blot analysis of extracts from various cell lines using CISD1/mitoNEET (D5M4C) Rabbit mAb.

Background

CDGSH iron-sulfur domain-containing protein 1 (CISD1), also known as mitochondrial outer membrane iron-sulfur (2Fe-2S) protein (mitoNEET) was first identified as the target for the drug pioglitazone, used to treat diabetes (1-3). CISD1/mitoNEET regulates both mitochondrial iron transport into the matrix and mitochondrial respiratory capacity. It has also been shown to affect the dynamics of cellular and whole-body lipid homeostasis (2,4). Furthermore, research studies have shown that CISD1/mitoNEET is overexpressed in human epithelial breast cancer cells and it has been considered a potential chemotherapeutic target (6).