Bottom Line:
Thioalkalivibrio sp.The strain was isolated from a mixture of sediment samples obtained from different soda lakes located in the Kulunda Steppe (Altai, Russia) based on its extreme potassium carbonate tolerance as an enrichment method.The genome was sequenced within the Joint Genome Institute Community Sequencing Program, because of its relevance to the sustainable removal of sulfide from wastewater and gas streams.

ABSTRACTThioalkalivibrio sp. K90mix is an obligately chemolithoautotrophic, natronophilic sulfur-oxidizing bacterium (SOxB) belonging to the family Ectothiorhodospiraceae within the Gammaproteobacteria. The strain was isolated from a mixture of sediment samples obtained from different soda lakes located in the Kulunda Steppe (Altai, Russia) based on its extreme potassium carbonate tolerance as an enrichment method. Here we report the complete genome sequence of strain K90mix and its annotation. The genome was sequenced within the Joint Genome Institute Community Sequencing Program, because of its relevance to the sustainable removal of sulfide from wastewater and gas streams.

f2: Phylogenetic tree based on 16S rRNA sequences showing the phylogenetic position of Thioalkalivibrio sp. K90mix. The sequence was aligned to sequences stored in the SILVA database using the SINA Webaligner [24]. Subsequently, the aligned sequences were imported into ARB [25], and a neighbor joining tree was constructed. Sequences of members from the Alphaproteobacteria were used as outgroup, but were pruned from the tree. The scale bar indicates 1% sequence difference.

Mentions:
Thioalkalivibrio sp. K90mix has rod-shaped cells with a polar flagellum (Figure 1), that elongate at high concentrations of K+ (Figure 1b). The strain is obligately alkaliphilic with a pH optimum of 10 (Table 1). It can tolerate a salinity of 4.0 M total Na+, but has an optimum of 0.3 M, sulfide concentrations up to 1 mM and a temperature up to 40°C. It has a preference for carbonate and sulfate as counter-anions over chloride and, therefore must be called “natronophilic”, instead of “haloalkaliphilic”. It utilizes ammonia, nitrate and nitrite as a nitrogen source. On the basis of 16S rRNA gene sequencing the strain belongs to the genus Thioalkalivibrio within the Gammaproteobacteria with T. thiocyanoxidans and T. nitratis as the closest described species (Figure 2). Most of the yet undescribed Thioalkalivibrio isolates from hypersaline lakes of Siberia and Mongolia also belong to this core genetic cluster of the genus Thioalkalivibrio.

f2: Phylogenetic tree based on 16S rRNA sequences showing the phylogenetic position of Thioalkalivibrio sp. K90mix. The sequence was aligned to sequences stored in the SILVA database using the SINA Webaligner [24]. Subsequently, the aligned sequences were imported into ARB [25], and a neighbor joining tree was constructed. Sequences of members from the Alphaproteobacteria were used as outgroup, but were pruned from the tree. The scale bar indicates 1% sequence difference.

Mentions:
Thioalkalivibrio sp. K90mix has rod-shaped cells with a polar flagellum (Figure 1), that elongate at high concentrations of K+ (Figure 1b). The strain is obligately alkaliphilic with a pH optimum of 10 (Table 1). It can tolerate a salinity of 4.0 M total Na+, but has an optimum of 0.3 M, sulfide concentrations up to 1 mM and a temperature up to 40°C. It has a preference for carbonate and sulfate as counter-anions over chloride and, therefore must be called “natronophilic”, instead of “haloalkaliphilic”. It utilizes ammonia, nitrate and nitrite as a nitrogen source. On the basis of 16S rRNA gene sequencing the strain belongs to the genus Thioalkalivibrio within the Gammaproteobacteria with T. thiocyanoxidans and T. nitratis as the closest described species (Figure 2). Most of the yet undescribed Thioalkalivibrio isolates from hypersaline lakes of Siberia and Mongolia also belong to this core genetic cluster of the genus Thioalkalivibrio.

Bottom Line:
Thioalkalivibrio sp.The strain was isolated from a mixture of sediment samples obtained from different soda lakes located in the Kulunda Steppe (Altai, Russia) based on its extreme potassium carbonate tolerance as an enrichment method.The genome was sequenced within the Joint Genome Institute Community Sequencing Program, because of its relevance to the sustainable removal of sulfide from wastewater and gas streams.

ABSTRACTThioalkalivibrio sp. K90mix is an obligately chemolithoautotrophic, natronophilic sulfur-oxidizing bacterium (SOxB) belonging to the family Ectothiorhodospiraceae within the Gammaproteobacteria. The strain was isolated from a mixture of sediment samples obtained from different soda lakes located in the Kulunda Steppe (Altai, Russia) based on its extreme potassium carbonate tolerance as an enrichment method. Here we report the complete genome sequence of strain K90mix and its annotation. The genome was sequenced within the Joint Genome Institute Community Sequencing Program, because of its relevance to the sustainable removal of sulfide from wastewater and gas streams.