Abcam's Free Fatty Acid Quantification Kit provides a convenient, sensitive enzyme-based method for detecting the long-chain free fatty acids (FA) in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc. In this assay, FA are converted to their CoA derivatives (coenzyme A), which are subsequently oxidized, leading to formation of color/ fluorescence. Fatty acids can then be easily quantified by either colorimetric (spectrophotometry at λ= 570 nm) or fluorometric (at Ex/Em= 535/587 nm)Visit our FAQs page for tips and troubleshooting.

関連性Fatty acids are carboxylic acids with long hydrocarbon chains. The hydrocarbon chain length may vary from 8-30 carbons. Fatty acids play very important roles in normal metabolism and many disease development. They are precursors to a number of bioactive classes of compounds such as prostaglandins, leucotrienes and others, and have been implicated in diverse functions such as autism, immune system and inflammation response.

Plasma from yellow-bellied marmots (Marmota flaviventris) was processed according the protocol. A serial dilution of the plasma (shown) was compared to the standard curved (also shown) to determine the optimal dilution factor for subsequent assays.

No, the kit does not detect triglycerides or other molecules that have a fatty acid components. The triglycerides need an enzyme to convert them to free fatty acids and this is not present in any of the kit components.

You can test for endogoenous Acyl-CoAs in your samples by including sample wells without added ACS Reagent. In these wells, the long-chain free fatty acids will not be converted to coenzyme A, so if there are any interfering compounds including endogen...

Although we would suggest to use fresh tissue preferably, tissue that is snap-frozen in liquid nitrogen can be used too. The tissue can be ground and then lipid extraction can be done with choloroform-triton.

Blood needs to be collected using an anticoagulant such as EDTA, sodium citrate, sodium fluoride, or ammonium oxalate. Heparinized plasma is not the best choice as heparin could interfere with the assay.