QIAsymphony mericon Bacteria Kit

Suitable for bacterial DNA isolation from all relevant food and environmental samples

DNA isolation from Gram-negative or Gram-positive bacteria with one protocol

No inhibitor carryover, so pathogen DNA is ready for use in downstream applications

Increases productivity by minimizing hands-on time

Easy tracking of samples and reagents using bar codes

The QIAsymphony mericon Bacteria Kit is for automated isolation of bacterial DNA from primary enrichment cultures of food and environmental samples as part of a pathogen screening workflow. It is specifically designed for use on the QIAsymphony SP, and optimized for use with the mericon Pathogen Detection real-time PCR assays. It uses a single protocol that is suitable for DNA isolation from Gram-negative or Gram-positive bacteria and features high process safety, including an inhibitor removal step and bar code reading to enable full tracking of reagents and samples. Each kit has reagents and accessories for 360 reactions. Prefilled reagent cartridges and continuous sample loading reduce setup time and minimize manual handling.

QIAsymphony mericon Bacteria Kit are intended for molecular biology applications in food, animal feed, and pharmaceutical product testing. These products are not intended for the diagnosis, prevention, or treatment of a disease.

The QIAsymphony mericon Bacteria Kit is specifically designed for use on the QIAsymphony SP and optimized for use with the mericon Pathogen Detection real-time PCR assays. Up to 96 samples can be loaded onto the QIAsymphony SP at a time. The samples are processed in batches of 24 with a 53-minute processing time per batch.

Each kit contains reagents and accessories for 360 reactions. The reagents are provided in resealable reagent cartridges for maximum flexibility.

Principle

The kit was specifically designed for use on the QIAsymphony SP, which is a sample preparation instrument that is part of the modular QIAsymphony RGQ system. The automated protocol allows the isolation of bacterial DNA from primary enrichment cultures of food, animal feed, and environmental samples. Bacteria concentrated by filtration can also be used as a starting material. The protocol is based on proven magnetic-particle technology that allows the isolation of DNA from both Gram-negative and Gram-positive bacteria.

The DNA extraction technology of the QIAsymphony SP instrument combines the speed and efficiency of silica-based nucleic acid purification with the convenient handling of magnetic particles. The protocol involves a short thermal lysis procedure that includes an inhibitor removal step. This is followed by a highly effective and streamlined process of binding, washing, and elution, using silica-based magnetic particles in combination with optimized buffers. The isolated DNA is ready for use in downstream applications, such as real-time PCR-based pathogen detection.

A complete, automated workflow is possible on the QIAsymphony RGQ, as the eluates containing the purified pathogen DNA can be automatically transferred to the assay setup module (QIAsymphony AS). These assay plates can then be transferred to the Rotor-Gene Q thermal cycler for real-time PCR and data analysis.

Procedure

No pretreatment of the bacterial enrichment culture or filtration-concentrated bacteria is needed. Samples are extracted directly from the culture and loaded onto the QIAsymphony SP along with the reagents and plasticware. The instrument performs all of the steps of the pathogen DNA isolation procedure and can process up to 96 samples in a single run. The protocol comprises 4 steps: lysis, binding, washing, and elution. Worktable setup is rapid and the instrument allows continuous sample loading, which minimizes hands-on time for increased productivity.

Applications

The QIAsymphony mericon Bacteria Kit is for automated pathogen DNA isolation from primary enrichment cultures of food and environmental samples as part of a real-time PCR-based pathogen detection workflow.