*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function

This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Research Areas

Cell Biology, Cell Death, Invasion/Microenvironment

*You can search these to find other products in these research areas.

Background

Annexin V also know as endonnexin II(ENX2),or placental protein 4(PP4). Endonexin II is a member of the family of Ca(2+)-dependent phospholipid binding proteins, known as annexins. It bind to the phospholipids that are preferentially located on the cytosolic face of the plasma membrane. It has a relative molecular weight of about 35,000. The gene lies on mouse chromosome 3 in close linkage with the fibroblast growth factor 2(basic) gene and is syntenic with other genes known to have orthologous counterparts on human chromosome 4q. The PP4 cDNA encoded a protein of 320 amino acid residues. A single mRNA, approximately 1.6 kb long, was found to be expressed in human cell lines and placenta. PP4 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Customer Q&As

A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.

Q: Is your western blot protocol provided from the website applicable for all your antibodies?
Keyword: applications, WB

A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.

Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody?
Keyword: conjugation

A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com

Q: What should I use for negative control?

A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.

Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal

A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.

Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?

A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.

Q: What is the expected band size? Why is it different than the observed band size?

A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,

Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?

A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.

Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?

A: Check our protocols under the tech support tab.

Q: What are some alternative names that could be used to describe this product?

A: Some common names include but are not limited to annexin v antibody, annexin 5 antibody, annexin a5 antibody, annexin-5 antibody