The antigen is the orphan protein, Placenta expressed transcript (Plet)-1. Biochemical data indicated that Plet-1 is a glycosylphosphatidylinositol-anchored glycoprotein with N-linked carbohydrates in addition to other posttranslational modifications. Plet-1 is a a recently identified protein that as of yet has no known function and is not associated with any one family of proteins. Plet-1 is used as a cell surface marker present on stem/progenitor epithelial cells and in a number of tissues including thymus and skin, and on epithelial cell types in mammary gland, prostate, and pancreas.

Catalog #

169180

Applications

Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilutions

Western Blot: 1:100-1:1000

Immunohistochemistry: 1:25-1:200 with ABC as detection reagent

Optimal dilutions to be determined by the researcher.

Hybridoma

Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Clone Type

Monoclonal

Isotype

IgG2b

Clone No

33A10

Host

Rat

Source

Mouse

Concentration

Not Determined

Form

Supplied as a liquid, 0.09% sodium azide

Purity

Supernatant

Immunogen

Mouse mammary tumor cells

Specificity

Recognizes mouse Plet-1.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.