quantitative PCR

Hi!
We do quantitative PCR with an internal standard derived from the
original cDNA where a small fragment is removed by restriction enzyme
digestion. Both can be coamplified in the same reaction mix and compete
for the primers according to their molar ratio. If you take known
amounts of your internal standard, you can determine the amount of
wild type cDNA at the point where both PCR products are formed at equal
amounts. Although be warned that this method is rather labour-intensive.
Volker
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Dr. Volker Blaschke
Depts. of Biochemistry, Dermatology
Georg-August-University, Goettingen, Germany
Email: vblasch at gwdg.de
Tel: xx49 551 39 5959 / 5978