应用

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用

Ab评论

说明

WB

Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).Can be blocked with Human DDAH2 peptide (ab22866). Please note that DDAH2, according to NP_039268, has a consensus glycosylation site. Glycosylation can affect migration of proteins on SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press].

IHC-FoFr

Use at an assay dependent dilution. PubMed: 21408057

ICC/IF

Use at an assay dependent dilution.

靶标

功能

Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.

组织特异性

Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain.

Thank you for your enquiry.
This is a well sold product and has been around for quite a while and we are currently selling the third batch. Interestingly, the very first batch did react with a 40kDa band in lysates of Mouse Heart. This would be cons...