Mentions:
PPrs can be generated efficiently using a previously described embryoid body‐based 3D mouse ESC (mESC) differentiation system 20, 22. In this culture system, continuous neuroepithelia are readily produced within 5 days of differentiation and optic vesicle‐like structures appear around days 7–9 (Fig. 2 shows optic vesicle neuroepithelium at day 12). Retinal cell genesis proceeds in a sequence similar to normal retinal development with all neural retinal cell types being present and correctly organized in layers by day 29 of the differentiation procedure, a stage that has been shown to correlate with P4–P8 during mouse retinal development 22.

Mentions:
PPrs can be generated efficiently using a previously described embryoid body‐based 3D mouse ESC (mESC) differentiation system 20, 22. In this culture system, continuous neuroepithelia are readily produced within 5 days of differentiation and optic vesicle‐like structures appear around days 7–9 (Fig. 2 shows optic vesicle neuroepithelium at day 12). Retinal cell genesis proceeds in a sequence similar to normal retinal development with all neural retinal cell types being present and correctly organized in layers by day 29 of the differentiation procedure, a stage that has been shown to correlate with P4–P8 during mouse retinal development 22.