Abstract

Elucidating marker-trait associations would have fruitful implications in distinctness, uniformity, and stability (DUS) tests of new varieties required for both variety registration and granting plant breeders’ rights. As the number of new varieties with narrow genetic bases increases, the necessity for deployment of molecular markers to complement morphological DUS traits gets particular attention. We used simple sequence repeats (SSRs) and sequence related amplification polymorphisms (SRAPs) markers in association mapping of morphological traits in a collection of 143 barley landraces and advanced breeding lines. This panel represented a diverse and uniform sample in terms of both quantitative and categorical traits whilst it was structurally partitioned by number of ear rows (six- and two-rowed) and seasonal growth habit (winter and spring types) characteristics. SSRs were more powerful compared with SRAPs in separating six- and two-rowed genotypes based on both model-based Bayesian and neighbor joining clustering methods. A number of associated SSR and SRAP markers were found for 15 out of 36 DUS traits after considering Bonferroni correction through linear models (GLM and MLM) and chi-square-based tests (SA and AAT). This is also the first report of association of awn roughness and grain color with molecular markers in barley. Moreover, SSR marker BMAC0113 appeared associated with time of ear emergence (TEE), confirming previous findings. These markers could be beneficial to complement and speed up DUS testing of new varieties, as well as for improving management of barley reference collections.

UPOV, 1994. Guidelines for the conduct of tests for distinctness, uniformity and stability, barley (Hordeum vulgare L. sensu lato). International Union for the Protection of New Varieties of Plants, TG/19/10. http://www.upov.int/edocs/tgdocs/en/tg019.pdf

UPOV, 2013. Guidance on the use of biochemical and molecular markers in the examination of distinctness, uniformity and stability (DUS). International Union for the Protection of New Varieties of Plants, TGP/15. http://www.upov.int/edocs/tgpdocs/en/tgp_15.pdf