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Abstract

:
Two new phenolic compounds, epicaesalpin J and 7,10,11-trihydroxydracaenone, were isolated from the heartwood of Caesalpinia sappan L. Their structures were identified by spectroscopic analysis methods, such as 1D and 2D NMR, along with the high resolution mass spectral data. The NO inhibition activities of two new compounds and six known compounds were tested.

Keywords:

Caesalpinia sappan L.; dracaenone; epicaesalpin J; phenolic compounds

1. Introduction

Caesalpinia sappan L. (Leguminosae) is widely distributed in Thailand, Indonesia, Vietnam, Burma, India and South and Southwest China [1]. The dried heartwood of this plant, Sappan Lignum, is popularly used as a Traditional Chinese Medicine for the treatment of menorrhalgia, cardiovascular and cerebrovascular diseases [2]. Previous investigations revealed that the extract of Sappan Lignum presented diverse and remarkable bioactivities, and therefore could be used as an anti-inflammatory, antimicrobial, antihypertensive and antiatherogenic agent. Prompted by the promising pharmaceutical properties, extensive studies on the phytochemical constituents of Sappan Ligunum have been carried out, which has resulted in the separation of various components including homoisoflavonoids [3,4,5,6,7,8,9], diterpenoids [10], dibenzoxocins [11,12,13,14,15,16,17,18], and a lactone [19]. As a part of our continuing studies on C. sappan [20,21], we report herein the isolation and structural identification of two new compounds 1 and 2 (Figure 1). Moreover, the NO inhibition activities of these new compounds and six known compounds 3–8 (Figure 1) were tested.

Figure 1.
Structures of compounds 1–11.

Figure 1.
Structures of compounds 1–11.

2. Results and Discussion

Compound 1 was obtained as a colorless gum, +317.3 (c 0.30, MeOH), UV λmax (MeOH) nm: 212, 242. Its molecular formula was determined as C17H16O6 by HR-ESI-MS (found 317.1021 [M+H]+, calcd. 317.1025). The IR spectrum of 1 showed α,β-unsaturated ketone (1649 cm−1), aromatic ring (1595 cm−1) and hydroxyl group (3443 cm−1) absorptions. The 1H- and 13C-NMR spectra of 1 displayed the characteristic signals of a methoxyl group (δ 3.62), an oxymethylene (δ 4.18 and 3.79), an oxymethine (δ 3.48), an α,β-unsaturated ketone group, and a tetrasubstituted benzene ring, respectively. All protons and carbons were unambiguously assigned by 1D and 2D NMR experiments, including 1H-1H COSY, HSQC, and HMBC (Table 1, Figure 2). Comparison of the NMR data with those of the known compound, caesalpin J (3) [11,21], revealed that 1 possessed a similar skeleton. The major difference is the upfield shift of H-13 from δ 3.84 in 3 to δ 3.48 in 1, and the same phenomenon was also observed in the compounds haematoxin (10) and epihematoxin (9) [22], which suggested that 1 was a stereoisomer of 3. The absolute configuration of 3 had been established in [12] by an X-ray crystallographic study of its triacetate. According to this paper, the ring B and C in 3 were both in a chair conformation. The optical rotation of 1 (+371.3) was consistent with that of 3 (+445.0), which suggested that 1 had the same configuration. In the NOESY spectrum of 1, the cross peaks between H-13 and H-6β, and OMe-13 and H-8 unambiguously confirmed that 1 was a C-13 epimer of 3, and the methoxyl group at C-13 was α-oriented (Figure 3). Thus, the structure of 1 was fully elucidated, and it was named epicaesalpin J.

Table 1.1H-NMR (500 MHz) and 13C-NMR (125 MHz) data for 1 and 2 (δ in ppm and J in Hz).

Table 1.1H-NMR (500 MHz) and 13C-NMR (125 MHz) data for 1 and 2 (δ in ppm and J in Hz).

No.

1 (in DMSO-d6)

1 (in CD3OD)

2 (in CD3OD)

δC

δH

δC

δH

δC

δH

1

146.3

7.03 d (10.0)

149.0

7.16 d (10.0)

151.5

6.88 d (10.0)

2

129.2

6.46 dd (10.0, 1.5)

130.4

6.51 dd (10.0, 1.5)

128.5

6.45 dd (10.0, 1.5)

3

187.1

190.9

191.4

4

108.5

5.48 d (1.5)

109.8

5.57 d (1.5)

108.1

5.58 d (1.5)

4a

175.0

178.6

179.7

6

77.8

4.12 d (11.0)

79.8

4.18 d (11.0)

81.0

3.88 dd (11.0)

3.72 d (11.0)

3.79 d (11.0)

4.27 dd (11.0)

7

69.4

71.3

66.8

8

37.6

3.16 d (16.0)

39.0

3.30 d (16.0)

43.5

3.16 d (16.0)

2.83 d (16.0)

2.88 d (16.0)

3.10 d (16.0)

8a

122.6

124.2

129.5

9

115.4

6.52 s

116.6

6.59 s

116.9

6.64 s

10

145.0

146.6

146.8

11

143.6

145.2

145.1

12

112.9

6.26 s

114.1

6.35 s

113.4

6.44 s

12a

126.6

128.5

125.5

12b

51.1

53.5

47.8

13

82.7

3.44 s

84.8

3.48 s

41.1

1.99 dd (11.5)

2.34 dd (11.5)

OCH3

61.4

3.53 s

62.6

3.62 s

-

-

Compound 2 was obtained as a colorless gum, −152.6 (c 0.54, MeOH). Its molecular formula was determined as C16H14O5 by HR-ESI-MS (found 285.0765 [M−H]+, calcd. 285.0769). The 1H- and 13C-NMR data of 2 were similar to those of the known compound 7,10-dihydroxy-11-methoxydracaenone (11), except that 2 showed no methoxyl signals. Considering that the molecular weight of 2 was 30 Da less than that of 11, compound 2 was identified as 7,10,11-trihydroxydracaenone. According to [23], the absolute configuration of 11 was different from those of 3 and 1, and 11 had a C ring boat conformation. The optical rotation of 2 was −152.6, which was similar to that of 11 (-465.9). Moreover, in the NOESY spectrum, the cross peaks between H-4 and H-6β, and H-8 and H-6α unambiguously confirmed that 2 had the same configuration as 11, which was opposite of that of 1 and 3 (Figure 3). All protons and carbons were unambiguously assigned by 1D and 2D NMR experiments, including 1H-1H COSY, NOESY, HSQC, and HMBC (Table 1, Figure 2 and Figure 3). Thus, the structure of 2 was confirmed as 7,10,11-trihydroxydracaenone.

Figure 2.
Key HMBC correlations of compound 1 and 2.

Figure 2.
Key HMBC correlations of compound 1 and 2.

Figure 3.
Key NOESY correlations of compounds 1 and 2.

Figure 3.
Key NOESY correlations of compounds 1 and 2.

The dracaenone skeleton is uncommon in natural products, and only six compounds with this type of skeleton have been reported [11,22,23]. They are isolated from Caesalpinia sappan L. [11], Haematoxylon campechianum [22], and Dracaena loureiri Gagnep [23], although it is not unexpected that they exist in different genera, because it is believed that the dracaenone skeleton is the oxidative cyclization product of homoisoflavan in biogenetic pathway [24], so we deduced that 1 and 2 should be the oxidative products of 12 and 13, respectively (Scheme 1).

Two new compounds and six known compounds caesalpin J (3), 1-hydroxy-7-methylxanthone (4), 1,5-dihydroxyxanthone (5), 1,7-dihydroxyxanthone (6), butein (7), and sappanone A (8) were evaluated for their inhibitory activities against nitric oxide production in LPS-activated BV-2 microglia according to a previously described method [25]. Compounds 4, 6, 7, and 8 showed obvious inhibitory activity, with IC50 values that were lower than those of quercetin, the positive control (Table 2).

3.2. Plant Material

Sappan Lignum (the heartwood of Caesalpinia sappan L.) was purchased from the Anguo medicinal materials market, Hebei Province of China, in September 2010. The plant material was authenticated by one of the authors, Prof. Peng-Fei Tu (School of Pharmaceutical Sciences, Peking University) and a voucher specimen (No.M-6-(5)) was deposited at the Herbarium of Peking University Modern Research Center for Traditional Chinese Medicine.

A portion of EA (800 g) was subjected to silica gel column chromatography eluted with a step-wise gradient of CHCl3 and MeOH to obtain fractions 1–12. Fraction 5 was chromatographed on silica gel eluted with petroleum ether/EtOAc (3:1 to 1:1) to give subfractions 5a–f. Fraction 5e was separated by a silica gel column eluted with CHCl3/MeOH (25:1–10:1) to give 5ea–ed. Fraction 5ec was passed through Sephadex LH-20 eluted with CHCl3/MeOH (1:1), and further purified by semi-preparative HPLC (MeOH/H2O 85:15) to yield 1 (12.0 mg), along with 3 (10.0 mg). Fraction 8 was subjected to silica gel column chromatography eluted with CHCl3/Me2CO (10:1–1:1) to give 8a–h. Fraction 8d was subjected to a silica gel column eluted with CHCl3/MeOH (20:1–5:1) to give 8da–df. Fraction 8db was separated by a MCI column eluted with MeOH/H2O (30:70–100:0) to yield 2 (11.0 mg). The isolation of the known compounds had been reported in our previous papers [21,22].

The spectral data of known compounds were reported in our previous literatures [20,21].

3.5. Inhibition of NO Production in LPS-Stimulated BV-2 Microglia

The assay was performed according to a previously described method [25]. Each compound was dissolved in DMSO and further diluted in the medium to produce different concentrations with a maximum concentration of 50 μM. The absorbance was measured at 570 nm with a Multilabel Plate Reader (Sunrise TECAN, Männedorf, Switzerland). Cytotoxicity was determined with the MTT assay. Quercetin (Sigma-Aldrich, Foster City, CA, USA) was used as the positive control.

4. Conclusions

The chemical study of the heartwood of C. sappan resulted in the isolation of two new compounds: epicaesalpin J (1) and 7,10,11-trihydroxydracaenone (2). Compounds 1 and 2 both have the dracaenone skeleton, which is uncommon in natural products. The new compounds, together with six known phenolic compounds, were evaluated for NO production inhibitory activity in LPS-stimulated BV-2 microglia. Compounds 4, 6, 7, and 8 showed obvious inhibitory activity.

Acknowledgments

Financial support from the Natural Science Foundation of China (No. 81303253) is gratefully acknowledged.