Silver Hydrosol
Info

June 2001: Natural-Immunogenics Corp.

Purpose.

The purpose of this study was to compare and contrast the inhibition
of various types of silvers on both normal and antibiotic resistant
strains of Staphylococcus aureus ("Staph"). This was accomplished by
inoculating healthy Staph with silver on standard YT plates, then placing
them in a 37° C incubator overnight.

Materials and Methods.

Each 95mm sterile polystyrene plate was filled with 5mm of YT media
consisting of 0.5% sodium chloride, 0.6% yeast extract, 0.8% tryptone
and 2% agar. A small 3 mm scrape of Staph was re-suspended in sterile
18 MW lab water and diluted. To each 1 mL dilution of staph cells,
100ul of each silver was added and then left to sit for 7 minutes.
After the appointed time, five 10µl spots of each diluted strain
containing silver were arranged on a YT plate.

The YT media was autoclaved and poured into sterile plates and
allowed to dry.

An optical density (O.D590) of 0.135 was achieved by using a 3
mm scrape of both S-1 and S-2, which were re-suspended in 1250µl
of lab pure water. Then a 10:1 dilution series was performed on each
strain.

Silver was added to each dilution of S-1 and S-2; the cultures
were then agitated and allowed to sit until spotted onto the YT plate.
The exposure time of each dilution to the silver was seven minutes.
This protocol was repeated for each of the four different silver
materials.

Results

Qualitative results can easily be seen on each plate. The positive
control for S-1 grew out 4.5 spots, represented by (++++½) and
the positive control for S-2 grew out 4.5 spots when colloidal silver
was not present (++++½). A (-) represents no Staph grew on the
plate. The effectiveness of the silver products for each company can
be compared by the number of spots or +’s seen in the graph.

Inhibition of Staph

S-1

S-2

+ control

++++½

++++½

Sovereign Silver

½

-

XX4A

++½

++½

AgNO3

++++½

++++½

XX7D 10 ppm

++++½

++++½

XX7D 100 ppm

+++

+++

XX7D 200 ppm

++

+

Results of inhibition with various silver products at 7 Minutes

S-1

S-2

Discussion.

In this experiment Sovereign Silver (SS) almost totally eradicated
the bacteria, while XX4A at 30ppm and XX7D at 100 ppm and 200 ppm were
only partially effective. Silver nitrate and XX7D at 10 ppm appear
to be only marginally effective at inhibiting the growth of Staphylococcus
aureus.