The use of stimulants and narcotics in human sports is prevalent since ancient times. These drugs are prohibited for use only Âin competitionÂ in sports by World Anti-Doping Agency (WADA), due to relatively short onset of action and if taken immediately prior to the sport event, will cause a performance enhancing effect. Doping control involves initial screening of suspicious samples for a prohibited drug followed by more specific confirmatory method. The dope testing has to be accomplished in a defined time period while fulfilling relevant technical criteria, therefore a simple, high throughput & open analytical method allowing detection of maximum number of analytes is choice of every anti-doping laboratory. The present work provides a comprehensive, sensitive and selective GC-NPD/MSD method for the detection of 80 stimulants, narcotics & fewother drugs of abuse excreted in free formin human urine. The method utilizes the feasibility of combining both the detectors (MS & NPD) with one GC producing dual data in a single run for fast &more reliable identification. The sample preparation was performed by liquid-liquid extraction of alkalinised urine. The limit of detection (LOD) for all substances was between 25-100 ng/ml. The method has been successfully utilized for the testing of more than nineteen thousand samples since 2009.