UL-840

Nedd8 is covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. Useful for affinity binding of Nedd8 activating E1 enzyme, the Nedd8 carrier enzyme Ubch12, Nedd8 E3 ligases, and other proteins/enzymes that have an affinity for Nedd8. Nedd8 is poly-His6 tagged. The ubiquitin-like protein Nedd8 is conjugated to targets by the Nedd8-specific E1 activating enzyme (AppBp1/Uba3), the Ubch12 E2 enzyme, and the ROC1/Rbx1 RING finger E3 ligase. Nedd8 plays a critical regulatory role in cell proliferation and development, and modifies nearly all members of the cullin family.

Product Information

Stock:

0.5 ml Nedd8-agarose is supplied in a 1 ml total volume of Hepes buffered solution.