Overview

General notesAliquots are at a concentration of 1-2 mg/ml and contain between 100ug and 200ug of lysate, with a minimum of 100ug.
To run on SDS-PAGE under reducing conditions it is necessary for the end user to add reducing agent. Addition of 5% beta-mercaptoethanol is recommended (5ul beta-mercaptoethanol per 100ul lysate).

Background46C is a cell line, constructed in the laboratory of Austin Smith, in which a drug resistance gene is placed under the control of a Sox1 promoter. The cells used in this lysate were Sox1-positive neural progenitor cells selected using puromycin following growth of Mouse Embryonic Stem Cells in N2B27 neural differentiation media. The Sox1 selection technology is patented.

We do not sell cell lines, only lysates. Unfortunately I do not know where you can source 46C cell lines. You may try searching online for stem cell providers or consult the literature for which labs have access to...