​Single molecule microscopy allows nanometer localization of the detected emitters and subtle probing of their spatio temporal nano-environment including in living cells and tissues.We are developing several single-molecule based super-resolution strategies (also called "localization microscopy") to achieve fixed and live cell studies of endogenous proteins at ultra-high resolution.​In particular, we developed uPAINT microscopy, which is the first implementation of PAINT super-resolution microscopy in fixed and live cells including live neurons.In addition, uPAINT allowed studying functional receptors in interaction by combining super-resolution microscopy and single-molecule FRET.​Current projects aim developing such strategies for studies in thick tissues. ​