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Pseudomonas savastanoi pv. savastanoi NCPPB 3335 is a model for the study of the molecular basis of disease production and tumor formation in woody hosts, and its draft genome sequence has been recently obtained. Here we closed the sequence of the plasmid complement of this strain, composed of three circular molecules of 78, 357 nt (pPsv48A), 45, 220 nt (pPsv48B), and 42, 103 nt (pPsv48C), a ...
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Pseudomonas savastanoi pv. savastanoi NCPPB 3335 is a model for the study of the molecular basis of disease production and tumor formation in woody hosts, and its draft genome sequence has been recently obtained. Here we closed the sequence of the plasmid complement of this strain, composed of three circular molecules of 78, 357 nt (pPsv48A), 45, 220 nt (pPsv48B), and 42, 103 nt (pPsv48C), all belonging to the pPT23A-like family of plasmids widely distributed in the P. syringae complex. A total of 152 coding sequences were predicted in the plasmid complement, of which 38 are hypothetical proteins and seven correspond to putative virulence genes. Plasmid pPsv48A contains an incomplete Type IVB secretion system, the type III secretion system (T3SS) effector gene hopAF1, gene ptz, involved in cytokinin biosynthesis, and three copies of a gene highly conserved in plant-associated proteobacteria, which is preceded by a hrp box motif. A complete Type IVA secretion system, a well conserved origin of transfer (oriT), and a homolog of the T3SS effector gene hopAO1 are present in pPsv48B, while pPsv48C contains a gene with significant homology to isopentenyl-diphosphate delta-isomerase, type 1. Several potential mobile elements were found on the three plasmids, including three types of MITE, a derivative of IS801, and a new transposon effector, ISPsy30. Although the replication regions of these three plasmids are phylogenetically closely related, their structure is diverse, suggesting that the plasmid architecture results from an active exchange of sequences. Artificial inoculations of olive plants with mutants cured of plasmids pPsv48A and pPsv48B showed that pPsv48A is necessary for full virulence and for the development of mature xylem vessels within the knots; we were unable to obtain mutants cured of pPsv48C, which contains five putative toxin-antitoxin genes. [--]

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This research has been supported by the Spanish Plan Nacional I+D+I grants AGL2008-55311-CO2-01 and AGL2008-55311-CO2-02 (Ministerio de
Ciencia e Innovación; http://www.micinn.es/), co-financed by FEDER, and by grants ref. 57/2007 from the Departamento de Educación y Cultura, Gobierno de
Navarra, Spain (http://www.educacion.navarra.es/portal/) and ref. P08-CVI-03475 from the Junta de Andalucía, Spain (http://www.juntadeandalucia.es). IP-M was
awarded a Spanish Ministry of Education and Science fellowship (AP2002-3800).