The maximum wavelength of fully oxidized HRP was 402.22nm, and the wavelength corresponding to the fully reduced HRP was 433.74nm. The sample with the addition of 64μL sodium dithionite was the fully oxidized sample, and the sample with the addition of 84uL sodium dithionite was the fully reduced sample. Since the reaction was not done in an entirely closed environment, oxygen from the atmosphere reacted with our sample which explains the large volumes added to our samples.