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Maxwell® 16 Cell LEV Total RNA Purification Kit

The Maxwell® 16 Cell LEV Total RNA Purification Kit is used with the Maxwell® 16 LEV Instrument. This RNA purification procedure provides an easy method for efficient, automated purification of highly concentrated total RNA from cell samples. Total RNA can be isolated from cells grown either in suspension or attached to plates. Cells are collected and processed in up to 400μl volumes. Purified RNA for up to 16 samples is obtained in less than 45 minutes of hands-free instrument operation. No post-purification treatment with nuclease, cleanup or concentration is required to achieve superior performance in downstrea...

The Maxwell® 16 Cell LEV Total RNA Purification Kit is used with the Maxwell® 16 LEV Instrument. This RNA purification procedure provides an easy method for efficient, automated purification of highly concentrated total RNA from cell samples. Total RNA can be isolated from cells grown either in suspension or attached to plates. Cells are collected and processed in up to 400μl volumes. Purified RNA for up to 16 samples is obtained in less than 45 minutes of hands-free instrument operation. No post-purification treatment with nuclease, cleanup or concentration is required to achieve superior performance in downstream applications. The low elution volume (30–100μl) is used to generate purified RNA in a more concentrated format for use in downstream applications such as qRT-PCR, RT-PCR and cDNA synthesis.

Features - Benefits

Obtain Application-Ready RNA: For most sample types, eluted RNA concentration is directly ready for qRT-PCR. No post-purification concentration is required.

Achieve High Yield and High Concentration: High yields and high-concentration total RNA result in better performance in gene expression analysis applications. Protocols for a variety of new samples are continually being developed. Visit: www.promega.com/Maxwell16/ for up-to-date information.

LEV Plungers
Components

This product is available through the Promega Helix onsite stocking program in a room temperature cabinet. The program offers numerous convenient solutions to meet your lab's needs. The Helix Cabinet is available in two sizes: 7.9 cubic ft. or 25 cubic ft.

This product is available through the Promega Helix onsite stocking program in a room temperature cabinet. The program offers numerous convenient solutions to meet your lab's needs. The Helix Cabinet is available in two sizes: 7.9 cubic ft. or 25 cubic ft.

Storage Conditions

Store at 22–25°C.

For product intended use please see Patents & Disclaimers tab.

Figure 1. High concentration and yield using the Maxwell® 16 Cell LEV Total RNA Purification Kit.

Total RNA was purified from 5 × 105, 7.5 × 105 or 1 × 106 HEK 293, NIH/3T3 or Jurkat cells and eluted in 50µl of Nuclease-Free Water. The average yield (µg) is shown as a dotted line, and the average concentration (ng/µl) is shown as a solid line. Maxwell® 16 Cell LEV Total RNA Purification Kit processing capacity depends on cell type, cell physiology and amount of cells used.

Use Restrictions

AS1225 For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.AS6101, AS6201 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

AS1225 U.S. Pat. Nos. 6,027,945, 6,368,800 and 6,673,631 and other patents.

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.