About RNA Clean & Concentrator™-5

RNA Clean & Concentrator™-5 provides a simple and reliable method for the rapid preparation of up to 10 µg of high-quality RT-PCR-ready, DNA-free (R1013, R1014) RNA. This simple procedure is based on the use of a unique single-buffer system and Clean-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt).

The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin™ IC Column in as little as ≥6 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.

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"We used the RNA Clean & Concentrator columns during our high-throughput sequencing library preparation. We used them to remove enzymes and buffers between each enzymatic reaction. We also used the >200nt protocol to remove unligated cloning linkers."