Exhibition

Dynamic Light Scattering

Physicochemical characterization of prepared nanomaterials is an important factor for the analysisof biological activities using radiation scattering techniques [10,14,112]. DLS can probe the sizedistribution of small particles a scale ranging from submicron down to one nanometer in solution orsuspension [10,14,113]. Dynamic light scattering is a method that depends on the interaction of lightInt. J. Mol. Sci. 2016, 17, 1534 6 of 34with particles. This method can be used for the measurement of narrow particle size distributions,especially in the range of 2–500 nm [78]. Among the techniques for the characterization of nanoparticles,the most commonly used is DLS [114–116]. DLS measures the light scattered from a laser that passesthrough a colloid, and mostly relies on Rayleigh scattering from the suspended nanoparticles [117].

Next, the modulation of the scattered light intensity as a function of time is analyzed, and the

hydrodynamic size of particles can be determined [118–120]. To evaluate the toxic potential of anynanomaterial, its characterization in solution is essential [11]. Therefore; DLS is mainly used todetermine particle size and size distributions in aqueous or physiological solutions [12]. The sizeobtained from DLS is usually larger than TEM, which may be due to the influence of Brownian motion.DLS is a nondestructive method used to obtain the average diameter of nanoparticles dispersed inliquids. It has the special advantage of probing a large quantity of particles simultaneously; however,it has a number of sample-specific limitations [101,121].