Description:
The IL-6 receptor system consists of two chains, IL-6R alpha and gp130. The IL-6R alpha is the binding component speci c to the IL-6, but does not transduce a signal alone. On the contrary, gp130 transmits signals not only of IL-6, but also IL-6 related cytokines, i.e. LIF, OSM, CNTF, IL-11 and CT-1. The low af nity binding sites for IL-6, is composed of IL-6R alpha alone. At low concentrations, soluble IL-6R alpha acts as an agonist of IL-6 activity.

Applications:Suitable for use in Flow Cytometry, Immunohistochemistry, ELISA, Immunoprecipitation and Inhibition assays. Other applications not tested.

Recommended Dilutions:Flow Cytometry: 1:50 Use 10ul to label 10e6 cells in 100ul.Immunohistochemistry: FrozenOptimal dilutions to be determined by the researcher.

Storage and Stability::May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type

Isotype

Clone

Grade

Mab

IgG1

3H1836

Highly Purified

Size

Storage

Shipping

Source

Host

100ug

-20°C

Blue Ice

Human

Mouse

Concentration:

~1mg/ml

Immunogen:

CHO cells transfected with cDNA of the IL-6 receptor.

Purity:

Purified by Ion Exchange chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Removal of sodium azide is recommended prior to use in functional assays.

Specificity:

Recognizes soluble and membranous human IL-6R. This antibody has been shown to inhibit IL-6 mediated proliferation of XG-1 cells. It partially blocks binding of IL-6 to its receptor.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.