Performing gel electrophoresis and duplicate immunoblot assays to test new primary antibodies or antibody concentrations is time-consuming and expensive. Restore Western Blot Stripping Buffer eliminates this waste when detecting immunoblots with chemiluminescent Western blotting substrates. The reagent allows clean and efficient removal of primary and secondary antibodies from immunoblots without removing or damaging the immobilized antigen. This allows blots to be stripped and reprobed with confidence.

Highlights:

Saves time – no need to re-run gels and blots

Saves costly sample – re-probe the membrane using the same target sample

Effective – formulation is more efficient at stripping antibodies than homemade buffers

Gentle – does not damage the target antigen during stripping allowing efficient reprobing

Odor-free – no mercaptans means no acrid odors

Economical – less expensive than other commercial stripping buffers

Reuse a nitrocellulose or PVDF blot to detect a different target with a different primary antibody; Reprobe a blot to correct or optimize antibody concentrations that were ineffective the first time.