When incorporated into the protein of interest, epitope tags make protein detection and identification very robust and easy. A large number of different epitope tags are now available for use. BioLegend epitope tag antibodies, peptide tags, and affinity gels are extremely specific, highly characterized and generate clear, quantitative results. These epitope tag reagents are suitable for a wide variety of applications including Western Blot, Immunoprecipitation, Protein Purification, Flow Cytometry, and Immunofluorescence Microscopy.

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Common Epitope Tags

FAQs

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Affinity Gels and Matrices

Epitope tag antibodies can be conjugated to beads (agarose, sepharose, metal, etc.) in order facilitate isolation or immunoprecipitation of tagged proteins. BioLegend offers several affinity gels and matrices, including c-Myc, 6-His, DYKDDDDK, GST, GFP, and HA. These matrices can be re-used for multiple rounds of purification and display excellent specificity at an economical price.

SDS-PAGE gel image of protein purified from the 1st or 10th rounds by either BioLegend anti-DYKDDDDK agarose or a competitor’s equivalent product. Final lane contains ladder.

SDS-PAGE gel image of protein purified from the 1st to 10th rounds by BioLegend anti-c-Myc agarose. First and last lanes contain ladder. There is no loss of binding capacity even after ten rounds of re-use.

Epitope Tag Antibodies

The epitope tag antibodies we provide are extremely specific and provide outstanding performance for protein purification, Western blotting, and immunoprecipitation applications.

BioLegend's Posi-Tag Epitope Tag Protein can be utilized as a positive control in Western blotting experiments where many epitope tags are commonly used. Posi-Tag represents proteins fused in the following order: GST, T7, HSV, c-Myc, VSV, Glu-Glu, V5, E-tag, DYKDDDDK, S-tag, HA, and 6-His, and has an approximate molecular weight of 45 kDa.

We also offer recombinant peptide tags that can be used during competition experiments to ensure specificity of your antibody. Alternatively, these synthetic peptides can be used to help elute the corresponding tagged protein from its affinity matrix.

Western blot showing several BioLegend epitope tag antibodies and their detection of 1 μg of Posi-Tag Epitope Tag Protein. All monoclonal antibodies were used at a 1:1000 dilution. Poly9248 was used at a dilution of 1:50000.