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Multiphoton microscopy combined with fluorescence lifetime imaging microscopy (MPM-FLIM) is a technique that allows for imaging the lifetime of fluorescence created by two-photon excitation. It not only possesses the advantages of MPM such as large imaging depth, but also the strengths of FLIM including the ability to discriminate different fluorophores with similar emission spectra. In FLIM, the frequency-domain (FD) method, which relies on periodic intensity-modulated excitation light and i…

In cancer and all biomedical researches, we always need a microscope with better imaging precision, speed, functionality, and depth, so that the diagnostics and treatments of cancer and other diseases can be accurate, fast, and noninvasive. However, conventional fluorescence microscopes cannot satisfy these needs due to physical limits on resolution, speed, information, and depth. In this work, we aim to overcome these limits using novel super-resolution microscopy and high-speed quantitative…

To fundamentally understand biological and medical phenomena such as aging and diseases, we need the information of the microenvironment surrounding living cells. We propose the multiphoton fluorescence lifetime imaging microscopy (MPM-FLIM) platform with super-sensitivity and super-resolution capabilities to quantitatively obtain this information at the intracellular level. First, we present a simple MPM oxygen imaging probe compatible with aqueous biological media based on water-soluble rut…

Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit, but its universal application is not feasible due to its difficult implementation and high cost. In this research, we propose and demonstrate a new kind of super-resolution fluorescence microscopy that can be easily implemented and requires neither additional hardware nor complex post-processing. The microscopy is based on the principle o…

Super-resolution microscopy is broadening our in-depth understanding of cellular structure. However, super-resolution approaches are limited, for numerous reasons, from utilization in longer-term intravital imaging. We devised a combinatorial imaging technique that combines deconvolution with stepwise optical saturation microscopy (DeSOS) to circumvent this issue and image cells in their native physiological environment. Other than a traditional confocal or two-photon microscope, this approac…

As climate changes, coastal homeowners are potentially crucial actors in reducing the risks to property and human life from rising seas and increased hurricane activity. Absent strict, enforceable regulations mandating retrofitting of existing homes or major changes in homeowner insurance requirements, coastal resilience in a changing climate will largely reflect private, voluntary decisions of millions of individuals. However, research is scarce on the current risk level of U.S. coastal home…

As climate changes, coastal homeowners are potentially crucial actors in reducing the risks to property and human life from rising seas and increased hurricane activity. Absent strict, enforceable regulations mandating retrofitting of existing homes or major changes in homeowner insurance requirements, coastal resilience in a changing climate will largely reflect private, voluntary decisions of millions of individuals. However, research is scarce on the current risk level of U.S. coastal home…

As climate changes, coastal homeowners are potentially crucial actors in reducing the risks to property and human life from rising seas and increased hurricane activity. Absent strict, enforceable regulations mandating retrofitting of existing homes or major changes in homeowner insurance requirements, coastal resilience in a changing climate will largely reflect private, voluntary decisions of millions of individuals. However, research is scarce on the current risk level of U.S. coastal home…