Category: chytrid fungus

Much has happened since my last post from the islands a couple of months ago which accounts for the tardiness of this one. However, Rayna Bell, our Cornell PhD candidate did manage to post two videos via National Geographic while we were on the islands.

I was invited to speak in TEDxSão Tomé, a great honor, and so returned in mid-June. Readers should know that there is but one TAP flight to the islands per week via Lisbon so this is no small undertaking especially for a single lecture. TEDx was a wonderful experience, and I was able to meet with some the brightest young people from the islands and to “spread the biodiversity word” internationally as well.

In the meantime, back at the Academy, we have been assessing the results of our fieldwork on GG VII; below is an image of the 2013 team, along with some of our best local friends.

The tall Sao Tomean in the back row, and the woman on the far right are Quintino Quade and Roberta dos Santos, respectively; the gentleman in the wheelchair is Ned Seligman. All three work for an NGO called STeP UP which has interacted closely with our CAS teams since the very beginning, especially with our biodiversity education efforts.

The on-going project was recently presented and summarized at international meetings in New Orleans by Dr. Tom Daniel, our senior botanist. Courtesy Charlotte Pfeiffer, CAS.

Shortly after returning, I learned from two colleagues here at CAS, entomologists Dr. Paco Hita Garcia and Georg Fischer, that they had described a new ant species from Sao Tome back in 2010, but somehow forgotten to tell me! The members of GG I collected these along the trail from Bom Successo and Lagoa Amelia over 12 years ago!

Tetramorium renae, Photo by CAS Project Lab.

Miko Nadel, our lichenologist, has narrowed the focus of his MA dissertation to the fruticose lichens of the genus Usnea which are found at higher elevations on the islands. He ascended Pico Príncipe, and readers will recall that he was part of the team that ascended Pico do São Tomé during GG VI. He now has over 600 collections upon which to base the first survey of this group in the islands.

Usnea sp. M.Nadel phot. GG VII, Principe Id.

Miko’s major advisor at San Francisco State University is Dr. Dennis Desjardin, a world authority on mushrooms and veteran of GG II and III. The blog of a year ago (April: Mountains that Glow) featured Miko’s discovery of tiny glowing mushrooms on Pico Sao Tome and later, glowing mycelium (threadlike plant body of fungi) covering steep hillsides at around 1100 meters. This year our photographer, Andrew Stanbridge, returned to Macambrara, the second locality, and discovered larger whole mushrooms that glow, not just the vegetative bodies. The two images below are of the same unidentified mushrooms in daylight and at night.

A. Stanbridge phot. GG VII, Macambrara, Sao Tome.

Rayna Bell reports from Cornell: we have very strong evidence now that the two species [São Tomé giant treefrog and Oceanic treefrog] are hybridizing [on São Tomé] (individuals of intermediate size/color and lots of molecular data to back that up), and now the question is whether they have always exchanged migrants and still diverged in body size, coloration, and breeding site (divergence with geneflow) or if they were isolated in the past and have recently come back in to contact (allopatric speciation). Just as exciting is that Rayna has discovered that the Oceanic tree frog, Hyperolius molleri (right below), that has long been thought to occur on both islands, does not; i.e., although very similar to each other morphologically, the two island populations are very different genetically, contradicting earlier molecular work by one of my interns years ago! Rayna and one of her undergraduate students just published their GG VI findings of chytrid fungus on the Sao Tome Cobra bobo; this is only the second published incidence of the fungus on a caecilian species.

Dr. Tamas Szuts, our Hungarian spider expert, was able to make great collections of salticids (jumping spiders) and orb weavers (Araneidae). Tamas was particularly excited about his collections of the genus Pochyta (below), a problematic group within the family.

Pochyta sp. Phot. T. Szuts, GG VII

Many of Tamas’s specimens are still being identified, but he writes: I also made some interesting observations about their [Pochyta] life history: a specimen had camped on a leaf just above a Phallus muchroom which attracted some small flies. [The] specimen was observed to jump several times toward the flying or landing targets, and then climbing back onto the leaf with the aid of its dragline.
Tamas took the remarkable photos below.

T. Szuts photos. GG VII

Finally, since we returned in May I have received several remarkable photographs of leatherback sea turtles, Dermochelys coriacea, one of four species that nest on the beaches of São Tomé and Príncipe. In terms of mass, this is the largest reptile in the world. The largest female on record was 915 kg (just under 1 ton, and close to 3 meters long (9.8 feet)!

PARTNERS:
We are most grateful to Arlindo de Ceita Carvalho, Director General, Victor Bomfim, and Salvador Sousa Pontes of the Ministry of Environment, Republic of São Tomé and Príncipe for their continuing authorization to collect and export specimens for study, and to Ned Seligman, Roberta dos Santos and Quintino Quade of STePUP of Sao Tomehttp://www.stepup.st/, our “home away from home”. We gratefully acknowledge the support of the G. Lindsay Field Research Fund, Hagey Research Venture Fund of the California Academy of Sciences for largely funding our initial two expeditions (GG I, II). The Société de Conservation et Développement (SCD) and Africa’s Eden provided logistics, ground transportation and lodging (GG III-V), and special thanks for the generosity of private individuals who made the GG III-V expeditions possible: George G. Breed, Gerry F. Ohrstrom, Timothy M. Muller, Mrs. W. H. V. Brooke, Mr. and Mrs. Michael Murakami, Hon. Richard C. Livermore, Prof. & Mrs. Evan C. Evans III, Mr. and Mrs. Robert M. Taylor, Velma and Michael Schnoll, and Sheila Farr Nielsen; GG VI supporters include Bom Bom Island and the Omali Lodge for logistics and lodging, The Herbst Foundation, The “Blackhawk Gang,” the Docent Council of the California Academy of Sciences in honor of Kathleen Lilienthal, Bernard S. Schulte, Corinne W. Abel, Prof. & Mrs. Evan C. Evans III, Mr. and Mrs. John Sears, John S. Livermore and Elton Welke. GG VII has been funded by a very generous grant from The William K. Bowes Jr. Foundation, and substantial donations from Mrs. W.H.V.“D.A.” Brooke, Thomas B. Livermore, Rod C. M. Hall, Timothy M. Muller, Prof. and Mrs. Evan C. Evans, Mr. and Mrs. John L. Sullivan Jr., Clarence G. Donahue, Mr. and Mrs. John Sears, and a heartening number of “Coolies”, “Blackhawk Gang” returnees and members of the Academy Docent Council. Once again we are deeply grateful for the continued support of the Omali Lodge (São Tomé) and Bom Bom Island (Príncipe) for both logistics and lodging and especially for sponsoring part our education efforts for GG VII.
Our expeditions can be supported by tax-deductable donations to “California Academy of Sciences Gulf of Guinea Fund”

Summer has been extremely busy. Our irrepressible bryologist, Jim Shevock, comes into my lab almost weekly with new moss discoveries from GG VI. He says a new paper on Fissidens (the largest moss genus on Sâo Tomé and Príncipe) is almost finished and will be submitted for publication as soon as he and his colleagues (from the US, the Netherlands and Lisbon) complete a key to identification of the species. Recall that Jim nearly doubled the number of collections he made during GG IV… He thought he had seen everything! The mesa on Príncipe will be a primary target for our botanists on GG VII, next year (see below).

Phrynobatrachus leveleve – RCD phot, GG VI

A nice surprise from GG VI was that we finally got some nice, un-posed photographs of the Sâo Tomé puddle frog, Phrynobatrachus leveleve. Readers may recall that we described this new endemic species back in 2007 following GG II (leve leve means “take it easy” in the local language). Obviously the way to get good shots of these critters is at night!

Ptychadena newtoni. A. Stanbridge phot – GG VI

Another good find on a different night was Newton’s rocket frog (Ptychadena newtoni) at a new locality, Caxueira.

The creek at Caxueira. A. Stanbridge phot. – GG VI

In earlier days I was concerned that this species, endemic to Sâo Tomé, was on the wane due to human development, but it appears to be more widely distributed than we thought (see also Feb 2011 blog). Caxueira is not far from the city center.

Why do we collect plant and animal specimens? Why do we bring them home euthanized and preserved (or in the case of plants, pressed and dried), and why do we organize and store them for posterity? The easy answer is that we need to find out what they are, to identify them and describe them so we can communicate about them. We certainly cannot conserve or preserve or even talk about species if we do not know they exist. This is particularly important in the tropics where so many different species have evolved, and especially in areas like Sâo Tomé and Príncipe that have never been fully explored by biologists. An added note is that for a biologist to know that a species is new and undescribed, he has to know all the related species that it isn’t and then demonstrate it!

It is a fact that a lot of things in the tropics that look alike are not at all related; conversely, some critters that look radically different are, in fact, just variants of the same species.

The botanists of course confront similar questions. Below are two species of the genus Impatiens.

(l) T. Daniel phot – GG IV; (r) M. Nadel phot – GG VI

Both species are high elevation forms described a long time ago: I. manteroana is thought to be endemic to Príncipe, while I. thomensis is known only from Sâo Tomé. But are they really different species? And if so, are they each other’s closest relatives? We do not yet have material of the former, but this is a question we can answer next year through DNA analysis. The specimen on the right was photographed high on the Príncipe mesa, which is one of the reasons it is a target area for next year.

Below is an island example of two species that look very much alike but are definitely not the same:

D. Lin phots: GG I, GG II]

These are photographs of small leaf-litter skinks of the genus Afroablepharus. The specimen above was collected on Sâo Tomé during GG I and the one below came from Príncipe (GG II). While they look identical, they are actually two different species as shown by colleagues of ours who were working on the molecular level: extracting DNA from small bits of tissue (probably tail tips) the two species were shown to be genetically quite different. The one from Príncipe was described over 160 years ago (A. africanus), while the one above, from Sâo Tomé, remains unnamed. This is most frustrating as even though we know they are separate species, we cannot describe the new species yet because the Sâo Tomé animals from which the DNA was analyzed were not collected. It is a complicated situation that both groups of workers together are trying to resolve at this time.

Another example can be found in the island geckos about which I have written before.

From public presentation by E Miller. CAS Big Kahuna phot (same specimens from above and below).

For over one hundred years, the geckos from both islands that lack thumb nails were considered to be the same species, Hemidactylus greeffi, originally described from Sao Tome. Our same colleagues noted that the two were genetically different but again failed to take whole samples and so could not describe the Príncipe species as new. It was not until we closely examined specimens in our Academy collections from both islands that we found many morphological differences between the two, which strongly supported the genetic evidence of our colleagues. The animal on the right is now known as Hemidactylus principensis, yet another island endemic. As luck would have it, the paper was published while we were on Principe!

The smaller specimen on the right in both views is also what is known as the holotype; i.e., it is the single animal that is described in minute detail that becomes the “name bearer”. All geckos collected from the islands and identified as H. principensis will be based on the description of this particular specimen; holotypes are the most important specimens in any collection.

In our collection, which is probably the fifth largest in the world, all holotypes are housed separately and identified by a blue ribbon.

Another question often asked of museum scientists is “why do you have to collect so many?” The answer is that species vary; no two members of the same vertebrate species are identical. This is why we include additional specimens in a species description. While the holotype or “name bearer” is usually a single animal in a standard description, other members of the same purported species, hopefully from the same place, are also described in some detail in order to account for individual variation. These are usually designated as paratypes; in the Academy collections, they are always designated by red ribbons [above] and are the second-most important.

Yet another frequently asked question is, “do you have to kill the specimens?” The answer lies in the fact that not all characters (similarities and differences) are observable from the outside. With animal groups like frogs, one has to look deeper, and this is impossible with living specimens. Below is a collage of some of the sorts of characters I had to examine in determining the relationships between members of African tree frogs of the family Hyperoliidae— found in Africa, the Seychelles and Madagascar.

All RCD phots.

Notice that the x-ray in the lower right hand corner revealed to us that the two geckos mentioned above not only lack thumb nails, they lack the entire terminal bone of the thumb! (the new species, Hemidactylus principensis, is on the left). So far as we know, they are the only two members of the genus Hemidactylus, (90+ species) that exhibit this characteristic. This might suggest they are each other’s closest relatives, but we are in the process of determining that by further DNA analysis that includes other closely related species.

During GG VI we did another kind of collecting:

Rayna Bell, Cornell University. A. Stanbridge phot – GG VI

Notice that in her left hand, Rayna Bell is holding an adult Sao Tome giant treefrog (Hyperolius thomensis), while in her right she has a cotton swab. She swabbed the skin of each frog she collected a number of times in a number of places in order to detect the presence of chytrid fungus. The swab will also detect the actual infection load if the fungus is present. This is the first attempt at detecting the fungus on the islands of Sâo Tomé and Príncipe, and we do not yet have results. It is certainly present in other areas of Africa. Batrachochytridium dendrobatidis (Bd for short) is a fungus that has been implicated in the mass die-off of populations of frogs in many parts of the world. Frog skin is a living membrane through which gasses and water can freely pass; while the mechanism is not well-known, the fungus seems to totally disrupt these functions causing the demise of the infected frog.

Cross section of Bd infected frog skin. (A) are sporangia with zoospores visible. (B) tube through which zoospores are released to the environment. Phot courtesy of A. Pessier, U. Illinois

Another real value to collections is the fact that past history can be discovered through our specimens. It turns out that Bd can also be detected by swabbing alcohol preserved specimens regardless of age, although the resulting data are not quite so informative as samples from living material. Below is Dr. Dave Blackburn’s “chytrid crew” (mostly undergrad and graduate students) swabbing specimens collected from the Impenetrable Forest of Uganda many years ago. Dave is our new curator in herpetology and a real expert on Bd.

Dave Blackburn’s “chytrid crew”. D. Blackburn phot.

Every trip to these small amazing islands yields new discoveries. We are planning our next expedition for 2013 and excited at the prospect of the new stuff we will find.

Here’s the parting shot.

Autonomy Day in Principe, 2012 A. Stanbridge phot, GG VI

PARTNERS

We gratefully acknowledge the support of the G. Lindsay Field Research Fund, Hagey Research Venture Fund of the California Academy of Sciences, (GG I, II), the Société de Conservation et Développement (SCD) and Africa’s Eden for logistics, ground transportation and lodging (GG III-V), STePUP of Sao Tome http://www.stepup.st/, Arlindo de Ceita Carvalho, Director General, and Victor Bomfim, and Salvador Sousa Pontes of the Ministry of Environment, Republic of São Tomé and Príncipe for permission to collect and export specimens for study. Special thanks for the generosity of private individuals who made the GG III-V expeditions possible: George G. Breed, Gerry F. Ohrstrom, Timothy M. Muller, Mrs. W. H. V. Brooke, Mr. and Mrs. Michael Murakami, Hon. Richard C. Livermore, Prof. & Mrs. Evan C. Evans III, Mr. and Mrs. Robert M. Taylor, Velma and Michael Schnoll, and Sheila Farr Nielsen; GG VI supporters include HBD of Bom Bom and the Omali Lodge for logistics and lodging, The Herbst Foundation, The “Blackhawk Gang,” the Docent Council of the California Academy of Sciences in honor of Kathleen Lilienthal, Bernard S. Schulte, Corinne W. Abell, Prof. & Mrs. Evan C. Evans III, John and Judy Sears, John S. Livermore and Elton Welke.

Our expeditions can be supported by tax-deductable donations to “California Academy of Sciences Gulf of Guinea Fund”.