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Biotechnology Services

Sanger DNA Sequencing

DNA sequence information is of fundamental importance and is required by most research programs at St. Jude. The Hartwell Center offers Sanger DNA Sequencing in support of ~100 research programs and sequences over 200,000 samples a year. We use state of the art technology to provide you with high-throughput and high quality data.

Genotyping Analysis

The Genotyping labaratory provides custom genotyping using the Illumina BeadXpress system which can multi-plex from 48 to 384 SNP assays per sample simultaneously. The laboratory also provides the PowerPlex® 16 multiplex STR assay for DNA typing, human identity testing and tissue culture strain identification. Custom fragment size analysis is also available using the ABI 3730xl genetic analyzers.

Genotyping

The Genotyping labaratory provides custom genotyping using the Illumina BeadXpress system which can multi-plex from 48 to 384 SNP assays per sample simultaneously. The laboratory also provides the PowerPlex® 16 multiplex STR assay for DNA typing, human identity testing and tissue culture strain identification. Custom fragment size analysis is also available using the ABI 3730xl genetic analyzers.

Clinical Applications Core Technology (CACT)

The Clinical Applications Core Technology (CACT) laboratory provides state of the art Affymetrix GeneChip® and Illumina BeadArray technology for analysis of gene expression, gene regulation, and genetic variation (SNP and CNV analysis). The laboratory offers both single sample analysis on cartridges or multi-sample analysis using PEG arrays on the Affymetrix GeneTitan system. Services include experiment consultation, sample processing, data acquisition and analysis.

Molecular Interaction Analysis

The Molecular Interaction Analysis laboratory provides quantitative characterization of proteins and protein interactions on surfaces (Surface Plasmon Resonance) and in solution (Analytical Ultracentrifugation and Multi-angle Light Scattering). These technologies offer an extensive range of analyses including real-time biomolecular interaction analysis, as well as size-and-shape distribution and interactions of protein complexes in solution.