Figure 4.

Fasting modulates the temporal regulation of circadian exons, and their relationship
with transcript-level expression. (a) Schematic depicting the timing of important events during the two days of the fasting
experiment; one day of LD followed by one day of DD. Colored boxes each depict 12
hour periods in the lighting schedule. White, day/lights on; black, night/dim red
light; dark grey, subjective day/dim red light. ZT, Zeitgeber time (the time under
an LD cycle); CT, circadian time (the corresponding time under a DD cycle). (b) Alternative splicing (AS) of four selected circadian exons. (c) Transcript-level expression for the same exons. For each combination of time point
and feeding status, n = 3 and error bars represent standard error of the mean. The symbols above each pair
of bars indicate the results of Bonferroni's post hoc test, comparing the two circadian time points in each feeding condition after a two-way
ANOVA: Ns, not significant; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.