Product Details anti-STAT1 Antibody

This antibody detects STAT1 alpha (C-term) at a 91 kDa band. No reaction to the 84 kDa STAT1a.

Cross-Reactivity (Details)

Species reactivity (tested):Human, mouse, rat

Purification

DEAE purified

Immunogen

STAT1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human STAT1 alpha. Remarks: The sequences differ from the murine corresponding sequences by four amino acids.

Isotype

IgG

Target Details STAT1

STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5alpha, and Stat5beta have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-alpha, IFN-beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). STAT1 has two forms, the 91 kDa STAT1alpha and the 84 kDa STAT1beta which are encoded by the same gene with splicing variant.Synonyms: Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84

Application Details

ELISA. Western blot: 1: 1000 - 1: 2000. Immunoprecipitation: 2 - 4 μg. Immunoflourescence. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Schindler, Fu, Improta, Aebersold, Darnell: "Proteins of transcription factor ISGF-3: one gene encodes the 91-and 84-kDa ISGF-3 proteins that are activated by interferon alpha." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 16, pp. 7836-9, 1992