A San Jose police officer accused of raping a woman while on duty pleaded not guilty Tuesday to two new felony assault charges filed by the Santa Clara County District Attorney’s Office, a prosecutor said.

A San Jose police officer pleaded not guilty Monday in Santa Clara County Superior Court to raping a woman after driving her to a hotel to separate her from her husband in a domestic dispute last September.

A Santa Clara County Superior Court judge on Monday ordered a San Jose police officer charged with forcible rape to turn over his firearms and not come within 300 yards of the alleged victim, a prosecutor said.