I have used triton-X-100 with NP40 or some other non ionic detergent at an
excess of 9:1 and have ben able to remove sds to the level I desire. I usually
use it convert a denaturing IP to non denaturing conditions I guess it depends
on the properties of your protein of interest
MArk Bond wrote:
> Hi,
>> Does anyone know of an easy way to removal SDS from protein samples. I know
> you can get detergent out columns from genotech that remove free sds but how
> can i remove SDS bound to the protein? Can I add triton - x- 100 ? This
> should form micells and pull the SDS from the protein into the hydrophobic
> micells, shouldn't it? Can I do enzyme assays with the triton there?
>> Hope you can help
>> Please reply to my e-mail address = Mark.bond at bris.ac.uk>> Cheers
>> Mark Bond
--
Siddhartha S Mitra
Dept. Of Biological Sciences
Amherst Campus
State University of New York at Buffalo
Buffalo NY 14214
Tel: (lab) 716 645 3323
(home) 716 837 2507
email: ssmitra at acsu.buffalo.edu