This paper investigates the deflection of the steel-concrete composite truss beam (SCCTB) at the serviceability limit state. A precise solution for the distributed uplift force of the SCCTB, considering five different loading types, is first derived based on the differential and equilibrium equations. Furthermore, its approximate solution is proposed for practical applications. Subsequently, the shear slip effect corresponding to the shear stiffness of the stub connectors, uplift effect corresponding to the axial stiffness of the stub connectors and shear effect corresponding to the brace deformation of the steel truss are considered in the derivation of deflection. Formulae for estimating the SCCTB deflection are proposed. Moreover, based on the proposed formulae, a practical design method is developed to provide an effective and convenient tool for designers to estimate the SCCTB deflection. Flexure tests are carried out on three SCCTBs. It is observed that the SCCTB stiffness and ultimate load increase with an increase in the shear interaction factor. Finally, the reliability of the practical design method is accurately verified based on the available experimental results.