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Figure 5

Model for Rad50NBD “activation” to a binding competent state. Left, illustrates the “ground” state Rad50NBD with stable interactions between the hinge, extended signature helix, basic switch, and Q-loop. This state is in equilibrium (green reaction arrow) with an ensemble of “Dimerization Competent States,” middle, in which the stable interactions are starting to break and increased side chain dynamics are present. The effect of V156M, V160M, and R805E is to increase the population of this state, as denoted by the orange, purple, and blue reaction arrows. ATP binds to this state, and dimerization can subsequently occur, right. The process is reset by ATP hydrolysis and dimer dissociation.