The separating and analyzing methods of oxygenates in crude oil are summarized. The advantages and disadvantages of the existing analyzing methods of oxygenates in crude oil are pointed out. It is held that combining infrared spectroscopy, mass spectrometries and other modern physical analyzing methods to identify the oxygenates in crude oil is the future development trend, which provides a theoretical basis for the exploitation of heveay oil resevoirs and the petrochemical processes.