Fucci Cell Cycle Vectors

Clontech’s cell cycle reporter vectors let you monitor cell cycle progression in living cells, in real-time, without fixation. They even let you visualize cell shape.

Fucci Probes

Our cell cycle reporter vectors deliver fluorescent, ubiquitination–based, cell-cycle indicators (Fucci) that allow you to identify cells in various phases of the cell cycle. These Fucci probes contain Cdt1 or Geminin, proteins whose levels fluctuate differentially throughout the cell cycle: Cdt1 levels peak in G1 phase; as cells transition into S phase, Cdt1 levels fall and Geminin levels rise, remaining high until the cells are back in G1. Cells control Cdt1 and Geminin levels post-translationally, using ubiquitination to target the unwanted proteins for proteasomal degradation. To allow Geminin and Cdt1 to be easily visualized, each is expressed with a red or cyan Living Colors fluorescent tag. These probes allow precise, visual evaluation of the cell cycle phase.

Monitor Cell Cycle and Shape in Real-Time

Our Fucci cell cycle reporters let you label just the nucleus, or both the nucleus and cytoplasm, allowing visualization of cell shape. Although most Fucci probes label only cell nuclei, we offer a truncated version of hGeminin that is able to migrate to the cytoplasm between S and M phases, enabling the morphology of the cell to be visualized.

A Variety of Fucci Probes and Delivery Options

We offer retrovirus- and plasmid-based vectors for the delivery of a variety of Fucci probes. Each probe consists of a red or cyan fluorescent tag, and a deletion mutant of either hCdt1 or hGeminin. Our retroviral vectors can either be transfected as plasmids or transduced as retrovirus in a wide variety of mammalian cells. Once integrated into the host genome, these vectors provide stable, heritable expression of the probes.

The plasmid-based vector, pTRE-CellCycle, allows tightly controlled, tetracycline (Tet)-inducible expression of two Fucci probes from a bidirectional promoter that can be induced using any Tet-On or Tet-Off technology. Expression of these probes causes cell nuclei to turn red during G1 phase and cyan during S, G2, and M phases, allowing complete visual tracking of the cell cycle.

Features

See the complete morphology of the cell during simultaneous monitoring of cell cycle phase

Applications

Monitor cell cycle transitions from G1 through G2/S/M phase

Visualize cell morphology during cell cycle transition

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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