Quantitative immunohistochemical assays of the vermal region of the cerebellum and CA1 (show CA1 Proteins)-3 regions of the hippocampus revealed reduced numbers of neurons in general, as well as reduced numbers of Pur-alpha+-immunopositive neurons and dendrites in heterozygous Pur-alpha mice, compared to wild-type littermates

This study underlines the importance of Puralpha in the proliferation of neuronal precursor cells during postnatal brain development and suggest a role for Puralpha in the regulation of the expression and cellular distribution of dendritic and axonal proteins.

Data suggest that Puralpha has a role in nucleotide excision repair and the repair of UVC-induced DNA damage.

Identification of mrna/protein (mRNA) complexes and their association with rough endoplasmic reticulum equipped with a kinesin motor

Pura is essential for postnatal brain development and developmentally coupled cellular proliferation as revealed by genetic inactivation.

we also determined a region in Pur alpha which is required for the association with the purine rich element binding protein by using deletion mutants of Pur alpha

Puralpha has been shown to colocalize with cyclin A (show CCNA2 Proteins)/Cdk2 (show CDK2 Proteins) and to coimmunoprecipitate with cyclin A (show CCNA2 Proteins) during S-phase and we show that this interaction is mediated by a specific affinity of Puralpha for Cdk2 (show CDK2 Proteins).

analysis of of DNA binding and localized strand separation by Pur alpha and comparison with Pur beta (show PURB Proteins)

PURA may be a potential target of miR (show MLXIP Proteins)-144 and observed downregulation of PURA may be caused by increased expression of miR (show MLXIP Proteins)-144. The other predicted target of miR (show MLXIP Proteins)-144 SPRED1 (show SPRED1 Proteins), was found to be downregulated in 69 per cent EC tissues as compared to matched distant non-malignant tissues.

The results of this study emphasizes the importance of stress granules in ALS (show IGFALS Proteins) pathogenesis and identifies Pur-alpha as a novel regulator of cytoplasmic stress granules dynamics.

These findings provide definitive evidence for the role of PURA in causing a variable syndrome of neurodevelopmental delay, learning disability, neonatal hypotonia, feeding difficulties, abnormal movements and epilepsy in humans

PURA Protein Profile

Protein Summary

This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia.