Native Microorganism 3α-Hydroxysteroid Dehydrogenase

Cat No.

DIA-270

Description

In enzymology, a 3alpha-hydroxysteroid dehydrogenase (B-specific) (EC 1.1.1.50) is an enzyme that catalyzes the chemical reaction: androsterone + NAD(P)+ ↔ 5alpha-androstane-3,17-dione + NAD(P)H + H+. The 3 substRates of this enzyme are androsterone, NAD+, and NADP+, whereas its 4 products are 5alpha-androstane-3,17-dione, NADH, NADPH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor, more specifically it is part of the group of hydroxysteroid dehydrogenases.

Abbr

3α-Hydroxysteroid Dehydrogenase (Microorganism)

Source

Microorganism

Appearance

White powder, lyophilized

Enzyme Commission Number

EC 1.1.1.50

Activity

> 50U/mg protein

CAS No.

9028-56-2

Molecular Weight

28 kDa (SDS-PAGE)

Isoelectric point

7.5

pH Stability

4.5~10.5

Michaelis Constant

3.0x10-5 M (androsterone); 6.0x10-6 M (NAD+)

Unit Definition

One unit will oxidate one micromole of androsterone per min at pH 8.9 at 25 centigrade.