Tissue SectioningMake 4-µm sections and place on pre-cleaned and charged microscope slides.Heat in a tissue-drying oven for 45 minutes at 60°CDeparaffinizationWash slides in 3 changes of xylene – 5 minutes each at room temperature.

DehydrateThis method should only be used if the chromogen substrate is alcohol insoluble.Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.Wash slides in 3 changes of xylene – 1 minute each at room temperature.Apply coverslip