The heterozygous mutants have no overt phenotype. The homozygous mutants have an early lethal phenotype. About 60% of the homozygous mutants die around E15, the other live to term but usually die within 24 hours after thay are born.

Mammalian Phenotype Terms:(provided by MGI)Extend all MPTs assigned by genotype

aconitase and NADH-oxidoreductase (with CoQ as a substrate) activities are reduced by 35% and 45%, respectively, in hearts; however, mitochondrial fumerase and cytosolic aconitase and glutamine synthetase activities are not reduced

the speed of induction of permeability transition by calcium is significantly increased in heart mitochondria

Sod2tm1Cje/Sod2tm1Cje

treatment with Manganese 5, 10 ,15, 20-tetrakis (4-benzoic acid) porpryrin (MnTBAP, a superoxide dismutase mimetic that does not cross the blood-brain barrier) increases survival time to a mean life span of 16.4 days compared to 8.3 days without treatment

mice older than 12 days of age display symmetric spongiform changes in large areas of the cerebral cortex, primarily in the mid to lower layers of the grey matter and the immediate subcortical white matter

vacuoles are mostly present in the neuropil and occasionally in the neuronal perikaryon

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email
mmrrc@missouri.edu.

Appearance

Coat color: Black

Other:

Breeding

MMRRC Breeding System: Intra strain mating

Breeding Scheme(s):

Heterozygous female x Wildtype male

Wildtype female x Heterozygous male

Generation: N21+

Overall Breeding Performance: Normal

Reproductive Statistics

Viability and Fertility:

Female

Male

Homozygotes are viable:

Yes

Yes

Homozygotes are fertile:

Yes

Yes

Heterozygotes are fertile:

Yes

Yes

Age Reproductive Decline:

16 weeks

16 weeks

Average litter size: 6 - 8

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU).
A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded
to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain.
(Use the MMRRC Catalog Search to request more than one strain.)

MMRRC Item # - Description

DistributionFee/unit (US $)

Units

Notes

000202-MU-RESUS

Litter recovered from cryo-archive

$2,022.00

Non-Profit

Litter

Recovered litter1; additional fees for any special requests.

000202-MU-SPERM

Cryo-preserved spermatozoa

$437.00

Non-Profit

Aliquot

Approximate quantity.2

1 The distribution fee covers the
expense of resuscitating mice from the cryo-archive; you will
receive the resulting litter. The litter will contain at minimum
one mutant carrier; the actual number of animals and the gender
and genotype ratios will vary. (Typically, multiple breeder pairs
can be established from the recovered litter.) Prior to shipment,
the MMRRC will provide information about the animals recovered. If
you anticipate or find that you need to request specific
genotypes, genders or quantities of mice in excess of what is
likely from a resuscitated litter, you may discuss available
options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with
sufficient sperm to recover at least one litter of mice, as per
provided protocols, when performed at the MMRRC facility. The
MMRRC makes no guarantee concerning the success of these
procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient
number of embryos (in one or more vials and based on the transfer
success rate of the MMRRC facility) to transfer to at least two
recipients. The MMRRC makes no guarantee concerning embryo
transfer success experienced in the recipient investigator's
laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the
Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the
Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

The MMRRC is a collaborative effort, funded by grants from DPCPSI of the
NIH.