Microbiology Researchhttp://www.pagepress.org/journals/index.php/mr
<p><strong>Microbiology Research</strong> is an international, Open Access online-only, peer-reviewed journal publishing research papers in the areas of clinical microbiology, molecular and cellular biology, virology, parasitology, mycology and bacteriology. Microbiology Research publish editorials, original articles, reviews, and brief reports.</p>en-USPAGEPress has chosen to apply the <a href="http://creativecommons.org/licenses/by-nc/3.0/" target="_blank">Creative Commons Attribution NonCommercial 3.0 License</a> (CC BY-NC 3.0) to all manuscripts to be published. <br /><br />An Open Access Publication is one that meets the following two conditions:<br /><br /> 1. The author(s) and copyright holder(s) grant(s) to all users a free, irrevocable, worldwide, perpetual right of access to, and a license to copy, use, distribute, transmit and display the work publicly and to make and distribute derivative works, in any digital medium for any responsible purpose, subject to proper attribution of authorship, as well as the right to make small numbers of printed copies for their personal use.<br /> 2. A complete version of the work and all supplemental materials, including a copy of the permission as stated above, in a suitable standard electronic format is deposited immediately upon initial publication in at least one online repository that is supported by an academic institution, scholarly society, government agency, or other well-established organization that seeks to enable open access, unrestricted distribution, interoperability, and long-term archiving.<br /><br />Authors who publish with this journal agree to the following terms: 1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal. 2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal. 3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.emanuela.fusinato@pagepress.org (Emanuela Fusinato)tiziano.taccini@pagepress.org (Tiziano Taccini)Tue, 02 Sep 2014 16:38:12 +0200OJS 2.4.6.0http://blogs.law.harvard.edu/tech/rss60Standardization of lyophilization medium for Streptococcus thermophilus subjected to viability escalation on freeze dryinghttp://www.pagepress.org/journals/index.php/mr/article/view/5402
The objective of the present study is to develop a lyophilization medium for <em>Streptococcus thermophilus</em> (NCIM 2904) as the industrial exploitation of this bacterium totally depends upon preservation and lyophilization protocols. Protective effect of 18 compounds were observed individually and in combinations with different sugars, sugar alcohols, polymers, protein concentrates and buffers. Among all the protectants tested, ammonium citrate (1% w/w), K2HPO4 (1% w/w) and KH2PO4 (1% w/w) provided lowest protection to these bacterial cells while 10% (w/w) sodium caseinate, whey protein concentrate, sweet whey powder, and skim milk showed significant results in viability escalation. Survival in carbon sources like lactose, sucrose and maltodextrine was also favored maximally. Combination of sodium caseinate 10%, skim milk 5%, sucrose 5%, lactose 5% and mono sodium glutamate 1% in distilled water in ratio of 1:5 with <em>S. thermophilus</em> showed survival percentage of 96%.Rohit Sharma, Bhagwan S. Sanodiya, Gulab S. Thakur, Pallavi Jaiswal, Anjana Sharma, Prakash S. Bisen
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http://www.pagepress.org/journals/index.php/mr/article/view/5402Tue, 02 Sep 2014 16:35:33 +0200Evaluation of polymerase chain reaction assay for pathogenic Leptospira detection in stored urinehttp://www.pagepress.org/journals/index.php/mr/article/view/5427
Pathogenic <em>Leptospira</em> spp. is the etiological agent of leptospirosis, a worldwide zoonotic infectious disease that causes jaundice, hemorrhages, renal failure and abortion in susceptible species. Urine is one of the preferred clinical samples for the detection of the agent. However due to its reliability, detection of leptospires in stored samples is challenged. Here we evaluated the capability of a polymerase chain reaction (PCR) assay for detecting pathogenic leptospira DNA in a non-sterile collected urine, stored at different times and temperatures. Our results indicate that the PCR protocol used detect pathogenic leptospira DNA but not non-pathogenic serovars or other non-leptospiral microorganisms. The sensitivity of the assay was of 100<em> Leptospira interrogans</em> in 10 mL refrigerated neutralized urine within 72 h post collection. This protocol could be of considerable interest for public health workers, field veterinarians and laboratory scientists, in sampling and processing urine for the detection of pathogenic <em>Leptospira</em> spp.Carlos A. Rossetti, Victoria Boggia
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http://www.pagepress.org/journals/index.php/mr/article/view/5427Thu, 18 Dec 2014 17:44:04 +0100Effect of low power microwave radiation on pigment production in bacteriahttp://www.pagepress.org/journals/index.php/mr/article/view/5511
Effect of low power (90 W) microwave (MW) radiation (2450 MHz) on bacterial growth and pigment production was studied in three different bacteria. Microwave exposure of 2-6 min duration was able to alter growth and pigment production (prodigiosin production by <em>Serratia</em> <em>marcescens</em>, violacein production by <em>Chormobacterium</em> <em>violaceum</em>, and staphyloxanthin production by <em>Staphylococcus</em> <em>aureus</em>) in the test organisms significantly. In this study, pigment production was estimated in the cell population originated from microwave treated inoculum, and not directly in the MW treated cells. Thus the alterations in pigment production and/or secretion might have been transferred from the originally MW treated cells to their daughter cells (who did not receive direct MW exposure), indicating the mutagenic influence of microwave radiation. Heavy prodigiosin overproduction observed in one of the test tubes inoculated with microwave treated S. marcescens could not be sustained by daughter populations corresponding to that tube, indicating the reversible nature of microwave induced mutation(s). The microwave effects observed in this study largely seem to be of athermal nature, as the thermal effect was minimized by use of ice during the microwave treatment.Shreya Raval, Vimla Chaudhari, Haren Gosai, Vijay Kothari
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http://www.pagepress.org/journals/index.php/mr/article/view/5511Thu, 18 Dec 2014 18:02:47 +0100