Affymetrix DNA labelling for gene expression arrays

This protocol will allow you to perform the labelling of DNA ready for Affymetrix expression genechip analysis.

Workflow

Materials

BioPrime Random Genomic DNA Labeling kit

Do not store enzymes in a frost-free freezer.

Miscellaneous Reagents

Protocol

BioPrime Random Genomic DNA Labeling Protocol

Extract DNA from a single leaf of each of 4 Columbia and 4 Ler plants using Qiagen DNA easy plant prep kit and re-suspended in 100ul. To extract equal amount of DNA from the 50 – 100 mutant or wild type F2 plants, take 1 leaf (or equal amount of tissue) from each plant and freeze together in liquid nitrogen. Grind tissue together and use ~100mg powder in the Qiagen DNA easy kit. Resuspend DNA in 100ul.