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The effect of wine matrix ingredients and conditions on the headspace sampling of 3-alkyl-2-methoxypyrazines was investigated with solid-phase microextraction (SPME) and capillary gas chromatography, using a nitrogen phosphorus detector. Changes in the recovery of 3-ethyl-, isopropyl-, sec-butyl-, and isobutyl-2-methoxypyrazines from the static headspace of synthetic wine matrices spiked with 5 mug/L of each analyte were investigated and reported as a function of SPME fiber type, extraction time, and temperature. The influence of pH, ethanol, phenolics, and oak was studied. Divinylbenzene/carboxen/polyclimethylsiloxane (PDMS) SPME fibers at an extraction temperature of 50degreesC for 30 minutes with 30% (w/v) added sodium chloride resulted in the highest analyte recoveries. Although PDMS (100 mum) SPME fibers at an extraction temperature of 35degreesC for 30 minutes with 30% (w/v) added sodium chloride resulted in lower analyte recoveries, the fiber remained functional for 50 to 75 analyses after other coatings deteriorated. Changing the sample ethanol concentration from 0 to 20% (v/v) resulted in an exponential decrease in the recovered analytes. Below pH 2, there was extensive loss of the analytes in the headspace. No measurable impact on alkyl-methoxypyrazine headspace concentrations was observed with exposures to selected phenolics and to oak.