Results: 5

Summary of functional avidities for cytotoxicity for HSV-2-specific CD8 clones derived from five subjects. A summary of the pathway to obtaining T-cell clones is indicated at the bottom. Each spot represents an individual T-cell clone.

Representative dose-response curves for indicated two subjects used to establish functional avidities of HSV-2-specific CD8 clones for IFN-γ release. The concentration of peptide used to load APC is plotted versus the concentration of IFN-γ in supernatants at a standard responder to stimulator ratio. Each line is a distinct T-cell clone.

Representative dose-response curves for indicated two subjects used to establish functional avidities of HSV-2-specific CD8 clones for cytotoxicity. The concentration of peptide used to load APC is plotted versus the percent cytotoxicity at a standard effector to target ratio. Each line is a distinct T-cell clone.

Selective expression of TCRBV12-utilizing TCRs by HSV-2-specific CD8 T-cells. PBMC from three indicated subjects were analyzed for binding of anti-CD8 mAb, tetramer B7-RPR, and a mAb specific for TCRBV12. The middle column shows TCRBV12 expression by tetramer (+) CD8 (+) cells and the right column expression by similar, tetramer (-) cells. The proportions of cells in the indicated regions of the histograms are stated.

Selective use of TCRB gene families by HSV-2-specific CD8 T-cells. Portion of ethidium/agarose gel viewed by UV light after PCR reactions with cDNA derived from sorted tetramer B7-RPR cells from PBMC from subject 5491. Bands at the expected molecular weight were only noted for primers specific for TCRBV primers 6 and 8 (lane labels) when combined with a C-region primer.