Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

normal

Age

48 years

Gender

female

Ethnicity

Caucasian

Applications

The two lines provide tumor and normal counterparts for comparative in vitro studies.

Storage Conditions

liquid nitrogen vapor phase

Karyotype

modal number = 46; range = 32 to 46

Derivation

This normal skin fibroblast cell line was isolated from the same patient as Hs 895.T (ATCC CRL-7637), a melanoma.

Comments

Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subcultivation Ratio: 1:2 to 1:4Medium Renewal: Every 2 to three days

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.