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3.
<ul><li>The colouring used for the endospores is the Wirtz-Conklin tecnique </li></ul>Introduction and Majorities

4.
<ul><li>Subtilis </li></ul><ul><li>It’s an ubiquitous bacterium commonly recovered from water, soil, air, and decomposing plant residue </li></ul><ul><li>As a specie of the Bacillus , it can form an endospore providing protection against heat and dessication of the environment. </li></ul><ul><li>It produces a variety of proteases and other enzymes that enable it to degrade a variety of natural substrates. </li></ul>Introduction and Majorities

5.
<ul><li>Amylase </li></ul><ul><li>It´s a proteic hydrolytic enzime that allows the catabolism of the starch and others carbohidrates. </li></ul><ul><li>Catalyzes the rupture of the glycosidics bonds α 1-4 producing dextrines and a mix of maltose, isomaltose and glucose. </li></ul><ul><li>Three different forms: α , β and γ with a similar function </li></ul>Introduction and Majorities

10.
General Objective <ul><li>Isolate the amylase genes from B. subtilis to mutate the DNA sequence with the PCR error prone and determine a more functional and effective enzyme for industrial and economic proposes </li></ul>

27.
Reference Statement Accomplished y/n 11. D. Hanahan The recombinant plasmids were transformed into E. coli XL1Blue and HB101, using the Hanahan protocol Yes 12. K. A. Laderman, K. Asada, T. Uemori et al. The presence of -amylase gene was determined at 92°C using the 12/KI method as described previously Yes 14. M. Simonen and I. Palva At present, about 60% of the commercially available enzymes are produced by Bacillus species, mostly being homologous proteins that are naturally secreted in the growth medium such as alkaline proteases as washing agent or amylases for the starch industry No 15. F. Kunst, N. Ogasawara, I. Moszer et al. The advantage of B. subtilis 168 over other bacterial strains has been increased even more by the availability of the complete sequence of the B. subtilis 168 genome Yes

28.
Final Conclusions <ul><li>We have concluded that different strains of diverse species like B. Subtilis in this case, provides more power and effectiveness in enzymatic process like the catabolism of the starch, according to the amylase. </li></ul><ul><li>Proteing engineering techniques can improve the scientific research manipulating the DNA sequence for a better improvement in the proteic function of an enzyme, looking for different purposes. </li></ul>

29.
<ul><li>The error-prone PCR method could allow a benign mutagenesis of a target protein for clinical uses like medical treatments in diverse afectations, for example enzymatics mutations because of chromosomics defects. </li></ul><ul><li>The correct use of the transformation vector HB101 and the restriction enzymes fulfill the objective of the improvement in an catalytic function to develop new methods and techniques in the scientific research for clinical purposes, leaving behind commercial and economics interestings. </li></ul>Final Conclusions