Summary

Secondary Antibodies

Negative Isotype Controls

Useful Reagents

Positive Controls

Histology Controls

More Images

References

Reviews

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Mouse anti human GIF, clone 139.1A11, recognises gastric intrinsic factor, a glycoprotein produced by parietal cells in the stomach. It plays a role in the absorption of Vitamin B12. Deficiencies in intrinsic factor can cause the autoimmune disease pernicious anemia.

Intended Use

Target Species

Human

Product Form

Purified IgG - liquid

Reconstitution

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preservative Stabilisers

0.09% Sodium Azide (NaN3)

Purity

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Reagents In The Kit

Preparing The Antibody

Test Principle

Buffer Solution

Phosphate buffered saline

Storage

Store at +4oC or at -20oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name

Yes

No

Min Dilution

Max Dilution

ELISA

1/500

1/10000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.