A novel cytokine related to IL2 and IL15 was recently identified and designated IL-21 . The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. Both IL-21R and the gc are necessary for the IL-21 function. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.

Application Details

IL-21 receptor antibody can be used for detection of IL-21 receptor by Western blot at 0.5 to 1 µg/ml. (Optimal dilution should be determined by user.) An approximately 60 kDa band can be detected. Antibody can also be used for ELISA and might be suited for other applications not tested so far.

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.

Ozaki, Kikly, Michalovich, Young, Leonard: "Cloning of a type I cytokine receptor most related to the IL-2 receptor beta chain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 21, pp. 11439-44, 2000