Tools

Welcome to the new TAL Effector Nucleotide Targeter website! In addition to a new look, we have added more options to our tools to allow you to design custom TAL effectors that work with a variety of TAL effector/TAL effector nuclease architectures.

Choose the TAL Effector-Nucleotide Targeter tool you would like to use. For information and tips on using the tools, check out the Help page:

News / Changelog

The system was patched on March 14, 2018. The next patching is scheduled for April 11, 2018. Please note that the system is unavailable for a few hours on scheduled patching days.

02/14/2018

The system was patched and rebooted February 14, 2018. The next patching is scheduled for March 14, 2018. Please note that the system is unavailable for a few hours on scheduled patching days.

01/10/2018

The system was patched and rebooted January 10, 2018. The next patching is scheduled for February 14, 2018. Please note that the system is unavailable for a few hours on scheduled patching days.

10/11/2017

The system was patched and rebooted October 11, 2017. The next patching is scheduled for November 15, 2017. Please note that the system is unavailable for a few hours on scheduled patching days.

9/13/2017

The system was patched and rebooted September 13, 2017. The next patching is scheduled for October 11, 2017. Please note that the system is unavailable for a few hours on scheduled patching days.

7/18/2016

An issue that caused TALEN Targeter jobs with off-target counting to fail has been fixed.

2/27/2014

Notice: Counting off-targets in pre-loaded promoteromes is much slower than in full genomes and will be disabled in a future update. It is typically better to count targets in the full genome and then run Target Finder / Paired Target Finder on the promoterome for TALEs/TALENs with predicted targets.

1/31/2014

There is currently a known issue where off-target counting jobs will occasionally crash just after starting. If your log says your off-target counting job has started, and after a couple minutes does not say it is scanning for binding sites, please try resubmitting your job.

1/7/2014

Paired Target Finder has a new option to limit the search to heterodimer results only or homodimer results only.

Target Finder and Paired Target Finder now run 2x-6x faster.

12/16/2013

All tools accepting NCBI IDs should do a better job of recognizing text identifiers (e.g. GCA_000164945.1).

TAL Effector Targeter now supports off-target counting.

6/18/2013

The Golden Gate TAL assembly form on the Resources page has been updated to correctly list reagents and output correct plasmid DNA sequences when NH is used as an RVD

5/31/2013

The individual sequence size restriction for off-target counting on TALEN Targeter has been bumped from 300MB to 316MB to support the Sus scrofa genome from NCBI. If you encounter another genome that does't work for this reason, please let us know using the contact form.

5/24/2013

A new option is now available on TALEN Targeter, Target Finder, and Paired Target Finder to provide an NCBI assembly or nucleotide sequence ID. When this option is used, the tool will automatically download and use the sequence for the provided ID for off-target counting or target finding. A tutorial on how to find an ID to use is available here.