"Is this the real life? Is this just fantasy? We welcome @QueenWillRock and @adamlambert to this year's Oscars!," the Academy of Motion Picture Arts and Sciences announced on its official Twitter feed.

The band, whose late frontman Freddie Mercury is the subject of Bohemian Rhapsody, is now made up of original members Brian May and Roger Taylor with former American Idol star Adam Lambert taking on vocals.

Bohemian Rhapsody has five Academy Award nominations, including a best actor nod for Rami Malek, who takes on the role of Mercury in the film. Mercury died of AIDS in London in 1991 at the age of 45.

The movie has taken more than $US854 million ($A1.2 billion) at the global box office to become the most successful musical biopic ever.

Bohemian Rhapsody is not nominated for its songs or soundtrack, but other musical performances at the ceremony will include Lady Gaga and actor-director Bradley Cooper singing their Oscar-nominated song Shallow from the movie A Star Is Born,' while Bette Midler will sing best original song contender The Place Where Lost Things Go from Mary Poppins Returns.