Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

chronic lymphocytic leukemia (CLL)

Gender

female

Ethnicity

Black

Storage Conditions

liquid nitrogen vapor phase

Images

Clinical Data

Patient had Kaposi's sarcoma.

female

Black

Comments

Patient had Kaposi's sarcoma.

Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Protocol:Volumes used in this protocol are for 75 sq. cm. flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37.0°C to facilitate dispersal.

Transfer cell suspension to a centrifuge tube and centrifuge at approximately 125 xg for 5 to 10 minutes. Discard supernatant.

Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37.0°C.

Subcultivation ratio: 1:2 to 1:3 every 7 to 10 days

Medium renewal: Every 3 to 4 days

Cryopreservation

Freeze medium: complete growth medium, 95%; DMSO, 5%

liquid nitrogen vapor phase

Culture Conditions

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

STR Profile

Amelogenin: X

CSF1PO: 10,12

D13S317: 11,12

D16S539: 10,13

D5S818: 12

D7S820: 8,10

THO1: 8,9

TPOX: 6,8

vWA: 15

Name of Depositor

Naval Biosciences Laboratory

Permits

These permits may be required for shipping this product:

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.