Principal Investigator

WAKANO Yoich The university of Tokushima School of Dentistry, Professor., 歯学部, 教授 (10028722)

Co-Investigator(Kenkyū-buntansha)

KATAOKA Masatoshi The university of Tokushima School of Dentistry, Research Associate., 歯学部, 助手 (20224438)
NAGATA Toshihiko The university of Tokushima University of Dental Hospital, Assistant Professor., 歯学部附属病院, 講師 (10127847)
ISHIDA Hiroshi The university of Tokushima School of Dentistry, Associate Professor., 歯学部, 助教授 (90127803)

We demonstrated the activity of phospholipase A_2 in human gingival crevicular fluid (GCF) associated with periodontal disease. Based upon the presence or absence of bleeding on probing (BOP), which is a marker for the disease activity, there were higher levels of the enzyme activity in BOP positive, than in negative sites. When the BOP positive sites became negative after periodontal therapy, the levels of enzyme activity decreased dramatically to almost undetectable levels. There were no significant differences between the activity before and after treatment when the BOP positive sites remained unchanged. These results suggest that the activity in GCF reflects periodontal disease conditions and that it can be a marker for disease activity.これらの結果は,GCF中のPLA_2活性測定が歯周病の状態を反映しており,将来disease activityを判定する指標の1つとして有効な方法になりうるものと考えられた。