Serves for phosphoproteomic inference of active phosphokinases. INKA utilizes label-free quantification of phosphopeptides derived from: kinases, kinase activation loops, kinase substrates deduced from prior experimental knowledge, and kinase substrates predicted from sequence motifs. This tool is able to identify active kinases, with potential clinical significance. It combines direct observations on phosphokinases, with observations on phosphoproteins that are known or predicted substrates for the pertinent kinase.