The RAW reporter cell line can be used for screening of TLR agonists or antagonists as well as inhibitory TLR antibody assay. RAW 264.7 cells are known to respond to most Toll-like receptor (TLR) ligands, which trigger the NF-kB induction and lead to inflammatory cytokine production. RT-PCR tests also shows that RAW 264.7 cells produce all of the TLR mRNAs except for TLR5 (Figure 1). Using a 96-well plate format assay, the RAW cell line has been validated by TLR ligand stimulation in which all of the TLR ligands except for TLR5 ligand activated the cell line (Figure 2).

Contents: 3~4 x 10^6 cells
Biosafety Level: 2

Immunogen

The RAW reporter stable cell line is a stably transfected RAW 264.7 cell line which expresses the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element.

Details for NFkB Secreted Alkaline Phosphatase Reporter

Type

Cell

Notes

Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed. - Use pipette aids to prevent ingestion and keep aerosols down to a minimum. - No eating, drinking or smoking while handling the RAW line. - Wash hands after handling the RAW line and before leaving the lab. - Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. - All waste should be considered hazardous. - Dispose of all liquid waste after each experiment and treat with bleach.

Alternate Names for NFkB Secreted Alkaline Phosphatase Reporter

SEAP assay

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.