Purpose::
To analyze the biocolonization properties of different porousmaterials with different compositions of polymeric acrylatesand methacrylates to find an ideal material to be used as thescaffold of a future keratoprosthesis.

Methods::
Adipose-Derived Adult Stem Cells (ADASC) and a cell line ofkeratocytes were cultured on different substrates with differentcontent of hydrophilic/hydrophobic monomers made of acrylatesand methacrylates (pEA and pHEA) at different ratios. Porousmaterials made of 100% pEA were also evaluated. 5.104 cellsper biomaterial in triplicates (1cm diameter and 1mm thick)were grown and their adhesion and proliferation rates analyzedby optical microscopy, SEM and Trypan Blue staining. Cells wereevaluated on days 0, 3, 5 and 7.

Results::
Proliferation of ADASC and keratocytes was observed on materialswith a main component of the hydrophobic poly ethylacrylate(pEA). The expansion and colonization of these cells on 100%pEA porous materials was only observed on the surface. Largecollagen deposits and producer cells covered the surface andno penetration was observed by SEM.

Conclusions::
Hydrophobic 100% pEA showed to be very efficient on adhesionand proliferation of ADASC and keratocytes on bi-dimensionalsubstrates. Porous materials made of 100% of this polymer didnot show colonization on the inside of the channels probablydue to the hydrophobic nature of this material. Future substratewill combine other hydrophilic materials to make the scaffoldsmore biocolonizable.