Alex Corbisiero, Tom Johnson and Freddie Burns were today all ruled out of England’s Six Nations opener against Scotland due to knee injuries and there are serious doubts over the fitness of Manu Tuilagi for the clash.

Fly-half Burns is out for at least two weeks, Johnson’s problem will keep the flanker on the sidelines for three months and Corbisiero has undergone keyhole surgery to remove a floating piece of bone.

The London Irish prop’s recovery means he will miss the Calcutta Cup clash at Twickenham on February 2 and the next game, in Ireland, the following weekend.

Tuilagi is recovering from an ankle injury and England’s medical staff will make a decision early next week.

There is also some concern over Saracens full-back Alex Goode, who must play against Cardiff this weekend to confirm his recovery from a shoulder injury but Tuilagi’s absence would have the biggest impact as it means a new midfield combination is required.

Owen Farrell could be switched to centre or London Irish centre Jonathan Joseph could start. George Ford will remain with the Saxons rather than being called up to replace Burns.

England head coach Stuart Lancaster said: “Freddie had a scan and it’s not as bad as first thought but Tom is going to be out for around three months.

“Alex will miss two games, minimum, but Manu has shown improvement in his ankle and could play against Scotland.”