Figure 4.

Early cytokine production kinetics by PBMC stimulated in vitro with LPS or SAC. Total cellular RNA was extracted from PBMC from 2 donors stimulated for 0.5, 1, 2,
3, 6 or 9 hours with LPS (blue bars) or SAC (red bars). Cytokine transcripts were
quantified using CyProQuant-PCR. The results are shown for one donor and were expressed
in mRNA copy numbers calculated relative to un-stimulated cells, after normalisation
against β2-MG (fold increase).