Disease:

Origin:

Species:

Tissue:

Spleen

Morphology:

Lymphoblast, hybridoma: B lymphocyte

Properties:

Suspension

Strain:

BALB/c (B cell); BALB/c (myeloma)

Medium:

AddexBio-Formulated DMEM (C0003-01) + 10% FBS.

Subculture:

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10^5 viable cells/ml.
Interval: Maintain cell density between 1 X 10^5 and 1 X 10^6 viable cells/ml.
Culture at 5% CO2, 37°C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO

Biosafety Level:

I

Sterility:

Bacteria: Negative

Yeast: Negative

Mycoplasma: Negative

Pathogens:

HIV: Negative

Hepatitis B: Negative

Citation Format:

If use of this culture results in a scientific publication, it should be cited in the publication as: QCRL-3 (AddexBio C0033010)