アプリケーション

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション

Abreviews

特記事項

IHC-P

1/50 - 1/100.

ICC/IF

Use a concentration of 1 - 2 µg/ml.

WB

1/100 - 1/500. Predicted molecular weight: 63 kDa.

ターゲット情報

機能

Protects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitement to dysfunctional mitochondria to initiate their degradation.

組織特異性

Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.

関連疾患

Defects in PINK1 are the cause of Parkinson disease type 6 (PARK6) [MIM:605909]. A neurodegenerative disorder characterized by parkinsonian signs such as rigidity, resting tremor and bradykinesia. A subset of patients manifest additional symptoms including hyperreflexia, autonomic instability, dementia and psychiatric disturbances. Symptoms show diurnal fluctuation and can improve after sleep.

Immunohistochemical analysis of paraffin-embedded Human stomach section, labelling PINK1 with ab75487 (1/25). An undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded Human heart section, labelling PINK1 with ab75487 (1/25). An undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunocytochemistry/Immunofluorescence analysis of PC-12 cells labelling PINK1 (green) with ab75487 at a dilution of 1/25. An Alexa Fluor® 488-conjugated goat anti-mouse IgG was used as the secondary antibody (1/400). Cytoplasmic actin was counterstained with Alexa Fluor® 555-conjugated with Phalloidin (red).

Immunocytochemistry/ Immunofluorescence - Anti-PINK1 antibody [38CT18.7] (ab75487)This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

Immunocytochemistry/Immunofluorescence analysis of PC12 cells labelling PINK1 with ab75487. Cells were fixed with paraformaldehyde, permeabilization with 0.3X Triton X-100 and blocked with 10% serum for 1 hour at 4°C. Cells were incubated with the primary antibody at 2 µg/ml for 24 hours at 4°C. An Alexa Fluor® 488-conjugated anti-mouse (1/1000) was used as the secondary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.