The LS 174T cell line was established from a 58-year-old Caucasian female patient who suffered from colorectal adenocarcinoma (a type of colon cancer). The cells exhibit epithelial morphology and are a variant of the LS 180 cell line. LS 174T is known to produce large amounts of carcinoembryonic antigen (CEA), and possess abundant microvilli and vacuoles. This cell line is positive for the expression of c-myc, N-myc, H-ras, N-ras, Myb, and fos oncogenes. This cell line is useful for the study of medical treatments related to colon cancer.

Figure 2. Protein expression of Cyclophilin B in LS-174T cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into LS-174T cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.