Beta-2-microglobulin (B2M) is found in the serum of normal individuals and in the urine in elevated amounts in patients with Wilson disease, cadmium poisoning, and other conditions leading to renal tubular dysfunction. Like immunoglobulins, prealbumin, and the beta protein found in the amyloid of Alzheimer disease, B2M has a predominantly beta-pleated sheet structure that may adopt the fibrillar configuration of amyloid in certain pathologic states. The protein is a single polypeptide chain of molecular weight 11,600 (human 119aa, chromosome 15). B2M associates with heavy chain of class I MHC antigens on the cell surface. A transient complex of MHC-heavy chain and B2M is known to be assembled into the TAP molecule involving interation with a number of chaperones. Binding of the processed peptide releases the class I-B2M complex to the cell surface. Absence of binding leads to degradation in the proteasome. Progressive hepatic iron overload, indistinguishable from that observed in HFE, was found only in mice homozygous for the mutated B2M gene.

Catalog #

M3890-03A

Applications

Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions

Flow Cytometry: 1ug/ml

Western Blot: 2-4ug/ml

Immunohistochemistry (Paraffin): 10ug/ml

Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Clone Type

Monoclonal

Isotype

IgG2a

Clone No

6D585

Host

Mouse

Source

Human

Concentration

~1mg/ml

Form

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

Purity

Purified by DEAE chromatography. ( 95% by SDS-PAGE)

Immunogen

Purified human b2-microglobulin.

Specificity

Recognizes human b2-microglobulin associated with cell-surface MHC Class I molecules and other membrane antigens as well as soluble human b2-microglobulin. Does not cross-react with mouse, bovine, rabbit, canine or chicken b2-microglobulin.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.