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The Invincibility Leaf Assist Block only appears after Mario loses five lives in a single stage. Once Mario collects the Invincibility Leaf, he transforms into White Tanooki Mario and becomes invincible for the entire stage. However, Luigi cannot obtain one unless Mario skips a normal world level (for example, by Warp Zone), as these blocks don't appear in the Special Worlds, and don't return if the level in question is beaten.

Exclusive to Super Mario 3D Land, the P-Wing Assist Block appears after Mario loses ten lives in a stage. Using the P-Wing takes Mario all the way to the end of the stage, in front of the Goal Pole.

In New Super Mario Bros. 2, only the Invincibility Leaf Assist Block returns. However, unlike in Super Mario 3D Land, the leaf inside turns Mario into White Raccoon Mario (rather than White Tanooki Mario), it will always appear if five lives are lost in a level (regardless of whether or not the stage in question has been beaten), and it will disappear if any stage with one is cleared without using it (restoring the sparkles on the current save file's stars as well).

The Invincibility Leaf Assist Block appears once more in Super Mario 3D World, working as it did in New Super Mario Bros. 2, though the White Tanooki form is retained from Super Mario 3D Land. However, the number of lost lives needed to make it appear is based on the number of players: five for one player, 12 for two, 18 for three, and 24 for four.