In this study, the concentrations of heavy metals Cu, Fe and Se in muscle, liver and gill tissues of S. guttatus were evaluated in Bushehr seaport during autumn 2016. S. guttatus samples were caught at random from Bushehr seaport (n=30). After biometric measurements the samples were taken from each tissues (muscles, liver and gills) and chemical digestion of the samples were carried out based on MOOPAM, then levels of heavy metals in tissues were measured by Graphite furnace atomic absorption spectrophotometer (Perkin Elmer 3030). Based on the results obtained the mean concentrations of heavy metals Cu, Fe and Se in muscles tissues of S. guttatus were 7.21, 3.07 and 0.094, in liver were 8.35, 3.02 and 0.107, and in gill were 9.87, 3.59 and 0.119 mg/kg dry weight respectively. Moreover, this results revealed that the Cu and Se concentration in muscles tissues of S.guttatus in Bushehr seaport were lower than the levels permitted within the standards of World Health Organization (WHO), Food and Agricultural Organization (FAO), National Health and Medical Research Council (Australia) (NHMRC), Ministry of Agriculture, Forestry and Fisheries UK (MAFF), But Fe concentration was higher than the level permitted within the standard of Food and Drug Administration (FDA).