Remove the comb from the cured gel and realign the gel in the chamber.

Adjust the buffer level by adding 1X TBE to the chamber until the buffer just covers the gel. The buffer can be reused a few times.

Pipette the samples into the wells

Apply 150 volts and run for approximately 60 minutes.

Photograph the gel using the UVP transilluminator system.

Notes

Our "1X TBE" is technically 0.5X TBE but for our purposes we call it 1X TBE.

Correspondingly, the 10X TBE is officially 5X.

Using TBE allows us to have such a high (150V) voltage. If you use TAE you need to use a significantly lower voltage and your run time will be longer. Despite that, TAE is advantagous in some cases, but Mike feels that TBE is better suited for his applications. This is discussed in detail in the consensus protocol.

Use the following table to determine the amount of agarose you want to use.