Start up from the OSX disc by holding down the 'C' key on powering on. From the top toolbar, select 'Disc Utility' from the 'Utilities' menu.Select the hard drive in the left hand column ans run 'First Aid'.This will diagnose and repair any problems with the hard drive unless the drive is beyond recovery, in which case you will need to replace the hard drive.

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Have you tried a PRAM start?
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