Target

FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Predicted band size : 92 kDaObserved band size : 92 kDaAdditional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

Immunoprecipitation - Apc4 antibody (ab72149)

Detection of Human Apc4 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72149 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.