Datasheet Extract

Application

Western blotting: Use 1/50 as starting working dilution.Immunoassays.

Background

The peptide antibiotic Polymyxin B (PMB) binds to bacterial endotoxin (lipopolysaccharide, LPS). The interaction of PMB with LPS involves ionic forces between amino groups in PMB and negatively charged phosphate and carboxyl groups in the lipid A-Kdo region. PMB has relevance for endotoxin research in at least two ways: first, PMB reacts with LPS of many species regardless of varied serospecificity, and thus it can be used as a general probe for measuring or detecting LPS or lipid A. Second, binding of PMB to LPS may result in neutralization of the detrimental effects of LPS either in vitro or in vivo.

Store the antibody undiluted at 2-8°C.Shelf life: one year from despatch.

Format

Preservatives:

0.02% Sodium Azide

State:

Culture Medium with a Low EndotoxinLow Endotoxin

Specificity

Specificity:

This Monoclonal antibody clone 45 reacts with Polymyxin B. It binds to free Polymyxin B as well as to Polymyxin B already bound to LPS. This antibody enables the possibilities to study quantitatively the interaction of PMB and LPS.