This is a test of behavioral “despair” or learned helplesssness [1, 2]. Essentially, a rodent is put in a tank of water from which there is no escape. The typical pattern of behavior is that the animals struggle and/or swim for some period of time. Eventually the animals “give up” and float. The latency to floating and the total time of floating are the typical measured parameters. While this may seem, at first glance, anthropomorhism, the test has been extensively validated. Firstly, it is sensitive to and predictive of the success of anti-depressants [3-9]. Secondly, many of the circumstances thought to promote depression in humans (unpredictable chronic stress, maternal and/or social isolation) also induce behavioral “despair” in rodents [5, 10-19]. Lastly, the physiological responses, the brain regions and underlying mechanisms regulating behavior is this test has similarity to those found in humans [2, 5, 10, 15, 20-32] and to other animals assays of depression [5, 30, 33, 34]. .