Product Details anti-IQGAP1 Antibody

Product Details anti-IQGAP1 Antibody

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Immunogen

Human IQGAP1

Clone

24

Isotype

IgG1

Cross-Reactivity

Mouse (Murine), Rat (Rattus), Dog (Canine)

Characteristics

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results. 2. Source of all serum proteins is from USDA inspected abattoirs located in the United States. 3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. 4. Please refer to us for technical protocols.

Purification

Purified from tissue culture supernatant or ascites by affinity chromatography.

Target Details IQGAP1

Target Details IQGAP1

GTPases (Ras, Rho, cdc42Hs, and rac) modulate various cellular functions such as cytoskeletal architecture, growth, motility, and gene expression. The activity of the GTP-binding proteins is regulated by factors that accelerate GTP-binding (GAPs) and proteins that enhance the rate of GTP hydrolysis (GTPases). IQGAP1 is Ras-GAP related protein that contains a WW domain that binds poly-proline regions, a calponin-homology domain (CHD), an IQ domain of unconventional myosins, and a C-terminal GAP-related domain (GRD). IQGAP1 co-immunoprecipitates with cdc42 and calmodulin, and colocalizes with cdc42Hs at the leading edges of the plasma membrane and at the cell-cell junctions. IQGAP1 may interact with both cadherins and catenins leading to dissociation of these proteins from sites of cell adhesion. Thus, IQGAP1 may be a Rac1 and Cdc42 effector that negatively regulates cadherin-mediated cell junctions.