This thesis details my investigation of some pharmaceuticals and natural products on the transport of drugs which are substrates of P-glycoprotein (P-gp), such as cyclosporin A (CSA) and digoxin by using Caco-2 and MDCKII-MDR1 drug transport models. Three objectives were performed to address this goal.... ml TrizolAr reagent (Gibco-BRL, Carlsbad, CA, USA) to the cells and processed
according to the manufacturera#39;s instructions. ... Hercules, CA, USA) using an ABI
Prism 7500 Real Time PCR system (Applied Biosystems, Foster City, CA, USA).