-

Image acquisition

-

Image processing

Processing

Method: single particle (icosahedral) reconstruction / Number of projections: 7085 / Number of class averages: 3Details: The particles were selected interactively at the computer terminal.Applied symmetry: I (icosahedral)

3D reconstruction

Algorithm: model-based common lines / Software: Purdue Suite / CTF correction: CTF correction of each particle. / Resolution: 9 A / Resolution method: FSC 0.5Euler angles: Euler angles (theta, phi, omega) are defined as three successive rotations in a right hand coordinate system. First, the viewer is rotated counterclockwise around the z-axis (angle 'phi') and then rotated counterclockwise around the new y-axis (angle 'theta') and rotate clockwise around the new z-axis (angle 'omega').Details: resolution determined by splitting data set into two groups.