Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution

ELISA: 1:100-200

Western Blot: 1:100-400

Immunocytochemistry: 1:100-500 (formalin fixed cells)

Immunohistochemistry: 1:100-500 (formalin fixed frozen sections)

Immunohistochemistry: 1:50-200 (paraffin sections)

Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Note

Applications are based on unconjugated antibody.

Clone Type

Polyclonal

Isotype

IgG

Host

Rabbit

Source

Human

Concentration

As reported

Form

Supplied as a liquid in PBS, pH 7.2. Conjugated to horseradish peroxidase (HRP). No preservative added.

Purity

Purified by immunoaffinity chromatography.

Immunogen

Recombinant H2AFY (Ile109~Asn369) expressed in E. coli.

Specificity

Recognizes human H2A Histone Family, Member Y (H2AFY).

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.