... get to about 750 nm (note that above 600 nm, you have to shift the lever at the lower left of the machine). 0.1M Phosphate buffer: 174 g K2HP04 (dibasic) brought to 1 liter with distilled H20 and 136 g KH2P04 (monobasic) brought to 1 liter with distilled H20 (monobasic has a lower pH). Mix some ...