2016-12-10T03:06:26ZMajority of TcRβ+ T-lymphocytes located in thymus and midgut of the bony fish, Dicentrarchus labrax (L.)http://hdl.handle.net/2067/1534
Title: Majority of TcRβ+ T-lymphocytes located in thymus and midgut of the bony fish, Dicentrarchus labrax (L.)
Authors: Romano, Nicla; Rossi, Francesca; Abelli, Luigi; Caccia, Elisabetta; Piergentili, Rita; Mastrolia, Lucia; Randelli, Elisa; Buonocore, Francesco
Abstract: Real-time polymerase chain reaction (PCR) and
in situ hybridization analyses were performed to investigate
the occurrence and distribution of T-lymphocytes expressing
TcRβ in intestine and lymphoid tissues of the bony fish,
Dicentrarchus labrax (sea bass). Immunohistochemistry
with the monoclonal antibody DLT15 (pan-T-cell marker)
was carried out to compare the cytology, distribution and
number of T-cells and TcRβ+ cells in the various sampled
lymphoid organs. The highest TcRβ expression was
revealed by real-time PCR in the thymus, with high levels
also being found in the gut. In the thymus, DLT15+ and
TcRβ+ cell populations were concentrated in the cortex and
TcRβ+ cells were notably reactive at the cortical-medullary
border, suggesting a specialized role of this region in
thymocyte selection. The density of DLT15+ T-cells increased
from the anterior to posterior intestine, whereas TcRβ+
lymphocytes were more numerous in the middle intestine
compared with other segments. The existence, in fish
thymus, of a medulla and a cortex comparable with those
of mammals is revealed by this study. The concentration of
TcRβ+ cells in the sea bass midgut also strongly suggests a
special role of this intestinal segment in antigen-specific
cellular immunity. The large population of TcRβ-/DLT15+
T-cells in the posterior gut can probably be ascribed to the
TcRγδ phenotype fraction.
Description: L'articolo è disponibile sul sito dell'editore http://www.springerlink.com2006-12-31T23:00:00Z