Native Thermotoga neopolitana β-Glucosidase

Cat No.

NATE-0771

Description

β-glucosidase is a glucosidase enzyme located in on the brush border of the small intestine that acts upon β1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). It is one of the cellulases, enzymes involved in the decomposition of cellulose and related polysaccharides; more specifically, an exocellulase with specificity for a variety of beta-D-glycoside substRates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of glucose.

Abbr

β-Glucosidase, Native (Thermotoga neopolitana)

Alias

β-glucosidase

Source

Thermotoga neopolitana

Applications

The enzyme may be used for hydrolysis of various glycosides such as polyphenol glycosides including naturally occurring antioxidants such as quercetin-glycosides found in various vegetables. For complete hydrolysis of 1 μmol of quercetin-4-glycoside in 5 minutes at 80°C and pH 5.5, about 28 pmol (~25 μg) enzyme was needed (Turner et al 2006)