As the wires needed to be planted into the ground, they took three seconds to arm, and were best used to eliminate roaming patrols, as well as proving useful for creating traps. For instance, as some Templar Den captains fled when they spotted the AssassinEzio Auditore da Firenze, a lethal trip-wire bomb could be placed on their route back to their hiding place.

In terms of bomb-crafting, trip-wire casings could be used in conjunction with any gunpowder type or ingredient.

Ezio and his allies were the only characters who did not trigger an explosion once they passed over a trip-wire bomb. Presumably, this could be explained with their experience of spotting and setting the bombs.

Due to this, civilians could set off a trip-wire bomb, potentially disrupting Ezio's plans.