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The calcium hardness of a water sample is found by complexation of Ca2+ with excess EDTA and back-titrating the excess with MgCl2.
A known amount of EDTA (0.0100 M, 25.00 mL) is added to a 20 mL aliquot of the water sample. (Y is used to symbolise the EDTA molecule)
Ca2+(aq) + Y4-(aq) <->...