Direct enzyme-linked immunosorbent assay (ELISA)HTML5

Summary

ELISA, Enzyme Linked Immunosorbent Assay, is a is an immuno-enzymatic technique that enables the detection of antigen-antibody reactions thanks to a reaction with colored products. ELISA is a technique currently used in the process of screening for HIV infections.

Learning goals

To understand the principle behind the use of the ELISA technique in screening for AIDS.

To describe the different steps invloved in the ELISA technique.

To point out the differences between direct ELISA and indirect ELISA.

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Antibodies (Ab) are secreted by the plasmacytes after a given antigen (Ag), a virus for example, is introduced into the organism. The presence of an antigen, or of its corresponding antibody, in a…