User:James C. Schwabacher/Notebook/CHEM-571/2013/11/13

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Objective

To run atomic absorption (AA) on our lysozyme and myoglobin and hemoglobin samples previously synthesized.

Procedure

Creating the Gold Stock Solutions

Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.

Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.

Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.

Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.

Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.

Atomic Absorption Samples

Solutions with the following Au:protein ratio were run on the AA:

Gold to Hemoglobin Ratio

30

50

70

90

110

130

150

170

190

210

Gold to Lysozyme Ratio

5

10

15

20

25

30

35

40

45

50

Gold to Lysozyme Ratio

30

50

70

90

110

130

150

170

190

210

Gold to Myoglobin Ratio

10

15

25

30

35

40

45

50

Figures

Notes

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