Flow (Intracellular): TLR4 Antibody (76B357.1) - An intracellular stain was performed on Jurkat cells with TLR4 antibody (76B357.1) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image from the Alexa Fluor 647 version of this antibody.