OriGene Data

Immunogen

A synthetic peptide corresponding to residues following the first double-stranded RNA-binding domain of human PKR was used as immunogen. The antibody does not cross-react with other GCN2 family members.

Clone Name

YE350

Isotype

IgG

Species Reactivity

Human (Does not react with: Mouse, Rat)

Concentration

Lot dependent; please refer to CoA along with shipment

Guaranteed Application *

WB, IHC, IF

Suggested Dilutions

WB: 1: 1,000-5,000; IHC: 1:250-500; ICC: 1:250-500; IP: 1: 100

Predicted MW

62 kDa

Why is the observed WB band size sometimes different from the predicted size? Common reasons might include:

Protein kinase R (PKR) is a serine/threonine kinase that is induced by interferon and activated by double-stranded RNA. Upon activation, PKR is autophosphorylated at multiple sites and phosphorylates the a-subunit of the eukaryotic initiation factor-2 eIF2 (eIF2a), inhibiting protein synthesis (1,2). PKR controls the activation of several transcription factors (1) and mediates the effects of interferon via its capacity to inhibit protein synthesis (2). It has been shown that the autophosphorylation sites Thr-446 and Thr-451 in the activation loop of Human PKR are critical for its kinase activity (3).