Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

IHC-P

Use at an assay dependent concentration.

This antibody is ready to use. For best results use heat mediated antigen retrieval, this can enhance staining if tissues are over-fixed. Do not use enzymatic mediated antigen retrieval. We recommend incubating sections with the antibody for 30-60 minutes at room temperature.

Target

FunctionGFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Tissue specificityExpressed in cells lacking fibronectin.

Involvement in diseaseDefects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.

This product is provided in a pre-diluted, ready-to-use format for immunostaining procedures. There is no need to dilute further this secondary antibody. Please refer to our IHC protocols for more information, and if you have any further questions, ple...