Proteome analysis of the human mitotic spindle.

Taxol-stabilized mitotic spindles were purified from HeLa S3 cells, and spindle protein content was analyzed by separation on 1DE, in-gel digestion, and nano-RP-LC-MS/MS, using parallel preparations in which microtubules were
disrupted by nocodazole as controls. 795 proteins were identified, of which 151 were known spindle components and 154 were previously uncharacterized proteins.

Expression of 17 candidates in the uncharacterized...

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