Tag Archive | CHAPTER 2

15 Chapter 2 – The Preoperative Evaluation James Blaugrund Dario Kunar Preoperative evaluation of surgical patients is, in its broadest sense, an extension of the diagnostic process. The surgeon should strive to determine the extent of disease; prove the necessity of surgery or clearly demonstrate its benefit to the patient; optimize the choice of surgical […]

Retroviral Vectors. The Moloney murine leukemia virus (MoMuLV) was the first virus used in developing a vector system for gene transfer. Parts of the retroviral genome that are involved in the replication of the MoMuLV—gag, pol, and env—are removed and replaced by a gene of interest. What remains of the retrovirus are the long terminal […]

PROTEIN-PROTEIN INTERACTION ASSAYS A number of different assays are used to determine and evaluate protein-protein interactions. Two in vitro assays are coimmunoprecipitation and polyhistidine-tagged glutathione sulfonyl transferase (GST) pull-down. Two in vivo assays are the yeast and mammalian two-site interaction assays. Coimmunoprecipitation. The commonly used coimmunoprecipitation assay makes use of antisera to specific proteins. In […]