Recombination in E. coli

Wolfgang Schechinger wrote:
> Hi all,
>> Just a question: When I transfect E. coli (HB 101) with a
> vector that contains an E. coli gene (cDNA for beta
> glucuronidase), could it be that recombination occurs?
>> I did a maxiprep but couldn't find any visible precipitate in the
> eluate. The bugs were growing in LB-Amp...
>> All input is welcome
>
Maybe too simple, but it happened once in our lab (we got no plasmid from
"supposed to be recombinant" cells): check the antibiotic.
Sergio