Fluorescein isothiocyanate (FITC) is a small uoresent molecule that is commonly conjugated to antibodies for immuno uorescent cell labeling. In conjunction with anti-FITC antibodies, it can also be used as an epitope tag for detection and signal ampli cation.

Catalog #

F5249-01

Applications

Suitable for use in Immunoblot and Enzyme Immunoassays.

Other applications have not been tested.

Recommened Dilution

Western Blot: 1:10-1:100 (Recommended substrate: BCIP).

ELISA: 1:100-1:500 (Recommended substrate: p-NPP ).

Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.