Biochemical testing of the organism will then reveal bileesculin positivity, PYRase negativity and no growth at 45 degree Celcius in NaCl (22,23). (thefreedictionary.com)

In confirmation tests, an aliquot of each isolate was then transferred to: (1) BileEsculin Agar (BEA) and incubated at 35[degrees]C, (2) BHI broth and incubated at 45[degrees]C and (3) BHI broth with 6. (thefreedictionary.com)

With an inoculating wire or loop, touch two or three morphologically similar streptococcal colonies and inoculate the slant of the bile esculin medium with an S-shaped motion, or streak the surface of a bile esculin plate for isolation. (microbeonline.com)