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Figure 3. LVST neurons presynaptic to hindlimb extensor MNs identified by transsynaptic retrograde tracing with RV. The top left shows a schematic overview of the location of the two main vestibulospinal groups, the ipsilateral LVST (iLVST) and contralateral MVST (cMVST) groups (both red), which were retrogradely labeled by RDA/BDA application at C1 on the right side (red arrow). Also shown is the unlabeled LVST group on the opposite side (cLVST, gray domain) and the side (right) on which RV was injected into the TS muscle group of the hindlimb (green arrow). IVth V, Fourth ventricle. A, Fluorescence micrograph of a transverse section through the brainstem 34 h after RV injection, showing cMVST neurons in the left vestibular region labeled with RDA/BDA (red). No RV-labeled neurons were detected in the left vestibular region at 34 h. The inset shows the location of image within the section. B, Fluorescence micrograph of a transverse section showing RDA/BDA-labeled (red) and RV-labeled (green) iLVST neurons in the right vestibular region. The inset shows the location of image within the section. C, Higher-magnification image of the boxed area in B, showing RDA/BDA-labeled LVST neurons (C1, red), RV-labeled LVST neurons (C2, green), and RDA/BDA/RV-double-labeled LVST neurons (C3, yellow arrowheads). p.i., Postinjection. Scale bars: 200 m (A, B), 50 m (C1, C2, C3).