Viral Vector and Transgenic Mouse Core

The overall goal of the VTMC is to provide DRC affiliate investigators with state-of-the-art vectors necessary to overexpress, knockdown, knockout, or alter expression of RNAs and proteins of interest in cultured cells, isolated tissues, and animals.

To accomplish this goal the VTMC provides the following to DRC affiliate investigators:

Production of lentivirus vectors, adeno associated virus (AAV) vectors, retrovirus (MMLV) vectors and adenovirus vectors for use in animals, tissues and cells;

CRISPR/Cas9-directed gene editing;

Production of lentiviral constructs for generation of transgenic and knockdown mice;

Production of non-viral vectors for generation of transgenic, knockout, and knockin mice;