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Scientific data relevant to the use of large number of plant species in folk medicines in the form of various types of products and their consequences on human health has been a major area of interest in the past few years. Ziziphus mauritiana (family: Rhamnaceae) being an important medicinal plant, posses tremendously beneficial properties attributed by the presence of different secondary metabolites or phytoconstituents. The present study was carried out to verify the antimutagenic potential of aqueous-ethanolic seed extract of Ziziphus mauritiana 2MS through Ames Salmonella plate incorporation assay using two strains of Salmonella typhimurium, TA 98 and TA 100. Our results revealed that ZMS has shown its activity against both the strains in a concentration dependent manner. Although, the results were more pronounced with TA 98 as compared to TA 100. At a concentration of 1000µg/0.1ml ZMS has shown 60% inhibition of mutagenesis with TA 100 while the same concentration has shown 66% inhibition of mutagenesis in TA 98. The results of co-incubaion and pre-incubation studes have revealed that with of both the strains extract was more effective in co-incubation study rather than pre-incubation study. Previous studies have shown the presence of terpenoids and other phytoconstituents, which may be responsible for the bioactivity.