Hal A. Lewis

Brookhaven National Laboratory
From: 1/5/99- 6/14/00

Past BNL Research Interests

I am the biology beamline scientist at x-ray beamline X25
at the National Synchrotron Light Source and as such am responsible for
the operation and development of the research facility for macromolecular
crystallography at X-25. This beamline posses a wiggler insertion device
making it the brightest among the biology-devoted NSLS x-ray beamlines
and so of great value to crystallographers with difficult crystallographic
problems.

Also, I'm continuing and expanding a collaboration with Professors R.
Darnell and S. Burley at Rockefeller University concerning the function
and structure of neuronal RNA-binding proteins
bound to their in vivo RNA ligands. This initially will entail solving
the co-structure of the Nova protein (discovered at Rockefeller) with its
RNA ligand then expand into the study of the FMR-1 protein and its RNA
ligand, an important area in the field of hereditary mental retardation.
This research will also contribute to our understanding of the various
ways proteins use to specifically recognize RNA sequences and structure.

Participation in the BNL/Rockefeller/Albert Einstein
Structural Genomics pilot.
The goal of this project is to rapidly determine one member of each family
of protein folds yet undiscovered as well as to develop the methodology
and instrumentation that will speed up the process of x-ray crystallographic
structure determination of proteins through the use of automated synchrotron
data collection.

Structure of a Nova KH domain. Ribbon diagram of Nova KH3 with conserved
aliphatic residues comprising the hydrophobic core illustrated in ball
and stick format. The invariant Gly-X-X-Gly is shown in yellow, and the
invariable loop in red. (a) a view of the beta-sheet face of the
KH domain. (b) A 90 degree rotation from the view in (a).
From H.A. Lewis et al.
Structure Fold Des., 7(2):91-203 (1999).

Last Modified: April 10, 2009Please forward all questions about this site to:
Kathy Folkers

One of ten national laboratories overseen and primarily funded by the Office of Science of the
U.S. Department of Energy (DOE), Brookhaven National Laboratory conducts research in the physical,
biomedical, and environmental sciences, as well as in energy technologies and national security.
Brookhaven Lab also builds and operates major scientific facilities available to university, industry
and government researchers. Brookhaven is operated and managed for DOE's Office of Science by Brookhaven
Science Associates, a limited-liability company founded by the Research Foundation for the State
University of New York on behalf of Stony Brook University, the largest academic user of Laboratory
facilities, and Battelle, a nonprofit, applied science and technology organization.