This antibody gave a positive signal in HepG2 as well as the following Human tissue lysates: Liver; Fetal Liver; Testis.
This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

性能

形式

Liquid

存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

存储溶液

pH: 7.40Preservative: 0.02% Sodium azideConstituent: PBSNote: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.

应用

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用

Ab评论

说明

ICC/IF

Use a concentration of 5 µg/ml.

WB

Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 43 kDa).

靶标

功能

Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.

组织特异性

Isoform 1 and isoform 2 are present in liver and testis.

序列相似性

Contains 2 laminin G-like domains.

细胞定位

Secreted. In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis.

Anti-Sex Hormone Binding Globulin antibody 图像

ab119436 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab119436 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.