[EN] The loss of viability in the storage of seeds has been a disadvantage for the conservation of
biodiversity in germplasm banks, as well as for the storage of seeds for agronomic use. The study of
the processes involved ...[+]

[EN] The loss of viability in the storage of seeds has been a disadvantage for the conservation of
biodiversity in germplasm banks, as well as for the storage of seeds for agronomic use. The study of
the processes involved in seed longevity could provide solutions to this problem. In particular, the
composition of the seed coat is important, since it supposes a natural barrier of protection against
harmful external agents and because it contains antioxidant molecules that repair the oxidative
damage caused during storage.
In this project, the study of the role played by seed coat flavonoids in the viability of the seed has
been initiated. For this, we have worked with a mutant seed collection of Arabidopsis thaliana with
different flavonoid composition. Through accelerated aging treatments, it has been investigated
whether the presence of certain types of flavonoids increases seed longevity. In addition, the
ability of these mutants to germinate in oxidant environments has been evaluated. The oxidative
damage produced after accelerated aging treatments has been approximated in some of these
mutants. Moreover, the permeability of tetrazolium salts intake has been measured and the layer
of suberin has been observed as a greater thickness of this layer implies greater impermeability.
The experiments suggested that flavonoids composition has a role in seed longevity, although they
are not essential and their increase does not mean an increment in seed longevity.[-]