Specific glucocorticoid receptor in human liver cytosol

Abstract: Specific cytoplasmic glucocorticoid receptors were isolated from biopsy material of liver tissue, obtained after operation of the patients with chronic impairment of bili ferous tract or from the patients with ulcerous disease of stomach within the stage of remission. The receptors isolated were saturated with dexamethasone; the dissociation constant value (Kdiss) of the complex was 1.37 10(-8) M and the amount of binding sites (n) was 1.96 10(-14) mol/mg of protein as calculated from a Scatchards' plot. Dexamethasone, corticosterol, hydrocortisone and deoxycorticosterol exhibited the highest competition for the binding sites of the receptor. The receptor of human liver studied was similar in its main properties to the dexamethasone-binding receptor from liver tissue of experimental animals.