The T98G cell line was originally established from the brain of a 61-year-old Caucasian male, who suffered from glioblastoma multiforme (GBM), the most common and most aggressive malignant primary brain tumor found in humans. This cell line exhibits fibroblast morphology and is not tumorigenic, but has a hyperpentaploid chromosome count (polyploidy). This cell line is a good transfection host, and is widely used for biomedical research related to treatments for brain tumors.

Figure 2. Protein expression of Cyclophilin B in T98G cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into T98G cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.