Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

IP

WB

Application notes

ELISA: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 0.1 - 1 µg/ml. Approx 85kDa band seen in Mouse Brain and Human Brain (Cerebellum) lysate [Predicted MW of approx. 80.8kDa according to NP_006449.2].

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

ab4215 immunoprecipitating RNF22 from rat brain tissue whole lysate. The antibody was used at a concentration of 2.5ug/mg of lysate. A Protein G matrix was used. The same antibody was used in the Western blot stage of the experiment (left hand panel).