David Rosen <davidr222 at aol.com> writes:
>>I am interested in assaying for and purifying antimicrobrial activity in
>plants. I would appreciate any information on the following-
>1. Preparing extracts from plants.
>2. Streamlining assaying.
>3. Initial characterization steps when dealing with an activity for
> which the type of molecule responsible is completely unknown.
>4. Useful journal articles (reviews?), books.
>5. Any other useful suggestions.
>>Please respond by e-mail to: davidr222 at aol.com
Just Curious, are you interested in antimicrobials as a natural defense
against plant diseases or for antibiotic use in humans and/or animals?
In any account, you might try starting your search by searching for references
on phytoalexins. These are antimicrobial compounds involved in induced
resistance responses in plants. A general but good discussion of phytoalexins
and some protocols on their extraction, purification, and bioassay can be
found in the chapter "Mechanism of Resistance" in Part III: Biochemistry
and host-parasite relationships in the text: METHODS IN PHYTOBACTERIOLOGY
edited by Z. Klement, K. Rudolph, D. C. Sands. 1990. Akademiai Kiado Pub.
Budapest, Hungary (Text is in English)/
Hope this helps some.
R.D. Gitaitis
Dept. of Plant Pathology
University of Georgia