A fusion protein of the light, oxygen and voltage-sensitive domain of the Avena Sativa phototropin 1 (LOV2) and the LacI domain. This transcriptional regulator represses promoters under control of LacI. It is speculated that in the presence of blue light, LacILOV dissociates, causing it to unbind from DNA and allow transcription. In the absence of light, the protein dimerizes and binds to the promoter of lac operon, inhibiting the transcription of downstream genes.

Gibson assembly of amplified LacILOV part from LacILOV-mCherry construct in pKDL071 and pSB1C3 backbone from LacILOV-mCherrt construct in pSCB1C3