Blockade of SHH pathway on wound healing in normal mice. The solution of SHH (5–20 μg/ml) or selective SHH receptor inhibitor cyclopamine (5 nmol/l) was directly placed on the wound surface for 30 min every other day from day 1 after wounding. The rate of wound closure was monitored every other day by tracing the wound area. Values were presented as means ± SE; n = 5. *P < 0.05 vs. control.

Decrease of endogenous SHH and PTCH 1 protein expressions in STZ-induced diabetic mice. Western blot analysis of cutaneous SHH and PTCH 1 was performed on day 0 and day 4 after wounding. Values were presented as means ± SE; n = 5. *P < 0.05 vs. day 0 (control).

Exogenous SHH on wound healing in STZ-induced diabetic mice. After STZ treatment (10 wk), a full-thickness excisional wound (1.5 × 1.5 cm) was made. The solution of SHH (20 μg/ml) or the selective SHH receptor inhibitor cyclopamine (5 nmol/l) was directly placed on the wound surface for 30 min every other day from day 1 after wounding. The rate of wound closure was monitored every other day by tracing the wound area. Values were presented as means ± SE; n = 5. P < 0.05 vs. control (*) and vs. STZ (#).