Available applications

Background of PEBP1 / RKIP antibody

Raf kise inhibitor protein (RKIP) is a member of the phosphatidylethanolamine-binding protein (PEBP) family that associates with Raf-1 and the MEK and MAP kises. RKIP has been shown to complex with Raf-1, MEK, and ERK. Although MEK and ERK can simultaneously bind RKIP, the association between Raf-1 and RKIP and that of RKIP and MEK are mutually exclusive. Thus, RKIP competitively disrupts the Raf-1-MEK complex and effectively termites sigl transmission from Raf-1 to MAP kises. The inhibitory effect of RKIP on MAP kise sigling is elimited by PKC phosphorylation of RKIP at Ser153. PKC phosphorylation on Ser153 also promotes the association of RKIP with GRK2, which prevents GRK2-dependent interlization of GPCR. RKIP also interacts with modules of the NF-?B pathway, including NF-?B-inducing kise (NIK), TAK1, IKKa and IKKß. These interactions antagonize cytokine-induced activation of the NF-?B pathway. Restoration of RKIP expression is associated with the inhibition of prostate cancer metastasis, implying that RKIP may be a potential clinical target as a suppressor of tumor metastasis through inhibition of vascular invasion.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with PEBP1 polyclonal antibody ( Cat # PAB3307 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with PEBP1 polyclonal antibody ( Cat # PAB3308 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.

(TOP)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (BUTTOM)Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PBP antibody (N-term) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

(LEFT) The anti-PBP Pab is used in Western blot to detect PBP in HeLa cell lysate. (RIGHT) Western blot analysis of anti-PBP Pab in Y79 cell line lysates (35ug/lane). PBP(arrow) was detected using the purified Pab (1:60dilution).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEBP1.

Immunofluorescent analysis of RKIP staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEBP1 (RC206355, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEBP1.