FAQ: Which culture media do you recommend?

We suggest using LB (Luria-Bertani) media for growth of E.coli. Rich media such as 2X YT or Terrific Broth can be used but typically result in significantly higher cell densities at saturation. Thus, it is necessary to scale back the amount of culture processed when using rich media to avoid insufficient alkaline lysis and column overloading due to higher biomass.