Transcription

1 NIH Public Access Author Manuscript Published in final edited form as: Nat Rev Cancer March ; 13(3): BAP1 and Cancer Michele Carbone *, University of Hawaii Cancer Center, 701 Ilalo Street, Honolulu, Hawaii 96813, USA; Department of Pathology at the University of Hawaii JABSOM Medical School, Honolulu, Hawaii 96813, USA Haining Yang, University of Hawaii Cancer Center, 701 Ilalo Street, Honolulu, Hawaii 96813, USA; Department of Pathology at the University of Hawaii JABSOM Medical School, Honolulu, Hawaii 96813, USA Harvey I. Pass, Department of Cardiothoracic Surgery, New York University, New York 10016, USA Thomas Krausz, Department of Pathology, University of Chicago, Illinois 60637, USA Joseph R. Testa, and Cancer Biology Program at the Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA Giovanni Gaudino University of Hawaii Cancer Center, 701 Ilalo Street, Honolulu, Hawaii 96813, USA Preface BAP1 is a deubiquitylase that is found associated with multi-protein complexes that regulate key cellular pathways, including the cell cycle, cellular differentiation, cell death, gluconeogenesis and the DNA damage response (DDR). Recent findings indicate that germline BAP1 mutations cause a novel cancer syndrome, characterized, at least in the affected families studied so far, by the onset at an early age of benign melanocytic skin tumours with mutated BAP1, and later in life by a high incidence of mesothelioma, uveal melanoma, cutaneous melanoma and possibly additional cancers. For the past 15 years we have investigated a mesothelioma epidemic in Cappadocia, Turkey, where we discovered that, in some families, susceptibility to mesothelioma, and possibly to mineral fibre carcinogenesis, was transmitted genetically in an autosomal dominant manner 1-5. These studies led us to search for a putative mesothelioma susceptibility gene or genes. To isolate these putative genes, we worked with two unrelated US families, referred as L and W, for their Louisiana and Wisconsin residency, that experienced a high incidence of mesothelioma and had only minimal potential exposure to asbestos 6. Our first important clinical observation was that two members in one of these families developed uveal melanoma (UVM): one of them died of the disease and the other was treated at an early stage and cured, but subsequently developed mesothelioma 4, 6. There are about 3000 mesotheliomas and a similar or smaller number of UVMs diagnosed annually in the US. The likelihood of both malignancies occurring in more than one individual in the same family was estimated at 36 per trillion 6 ; this linkage suggested a common genetic denominator. Chromosome microarray and genetic linkage analyses on the L and W families implicated * Corresponding Author: Michele Carbone, 701 Ilalo Street, Honolulu, HI 96813, USA. Phone: , Fax: Competing Interests Statement. MC and JRT have a pending patent application on BAP1.

2 Carbone et al. Page 2 chromosome region 3p21 6, a locus frequently altered in both UVM 7 and mesotheliomas 8, 9. Sequencing this region of chromosome 3 led to the identification of BAP1 as the gene mutated and associated with high rates of mesothelioma in the L and W families (experiments are in progress to address whether BAP1 also causes the high incidence of mesothelioma in Cappadocia) 6. BAP1 is a member of the ubiquitin C-terminal hydrolases (UCH) subfamily of deubiquitylating enzymes (DUBs) and was mutated in each family member who had developed mesothelioma, UVM and other cancers. None of these tumour types was detected in family members who did not carry BAP1 mutations 6. Moreover, among several cases of sporadic mesotheliomas, we found that 2/26 patients had germline BAP1 mutations 6 and importantly we found that both of these patients had been previously diagnosed with UVM. None of the 24 patients with sporadic mesothelioma who had wild-type germline BAP1 had been diagnosed with UVM. On the basis of these findings, we have proposed that germline BAP1 mutations cause a new cancer syndrome characterized by mesothelioma and UVM 6. In this Progress article, we discuss this potential inherited cancer syndrome, the possible mechanisms through which mutations in BAP1 might lead to tumour development and the clinical implications raised by detection of germline BAP1 mutations. Benign melanocytic tumours in BAP1 families In parallel with our paper 6, Wiesner et al. 10 reported that germline BAP1 mutations caused benign atypical melanocytic tumours. In a subsequent paper 11, they noted that these melanocytic lesions resembled histologically atypical Spitz tumours (ASTs). ASTs are a heterogeneous group of melanocytic tumours with overlapping histological characteristics between benign Spitz nevi and malignant melanoma and are formed by large melanocytes with mitotic activity not only in the superficial but also in the deep part of the lesion. They studied 32 sporadic ASTs and found that 9 had loss of BAP1 expression and 8 of these had concomitant BRAF mutations. Only 1 of 23 ASTs that expressed BAP1 had a BRAF mutation (P< ) 11. BRAF mutations are common in melanocytic nevi (80%) and in cutaneous melanoma (65%) but are rare in ASTs 12. Njauw et al. 13 noted the same type of skin tumours arising in families with germline BAP1 mutations and referred to them as nevoid melanoma-like melanocytic proliferations (NEMMPs). Thus, pathologists have used different names to identify these pink to tan skin tumors of about cm in diameter, which macroscopically resemble dermal nevi. Together with a team of dermatologists, pathologists and dermatopathologists at 3 different US National Cancer Institute (NCI)- designated Cancer Centers we reviewed the published morphology and histology of all of these melanocytic tumours, including some of the original tissue sections kindly provided by Weisner and colleagues 10. In addition, we examined the skin tumours we detected in the members of the L and W families. We found that all the melanocytic tumors in these BAP1 mutant patients had the same histological and molecular characteristics. In our review of the skin tumours in patients with mutant BAP1 we found that these are nevus-like lesions usually formed by a conventional junctional, compound or dermal nevus composed of small melanocytes expressing BAP1. Next to these conventional nevus cells there is a dermal lesion formed by large epithelioid BAP1 negative and most frequently BRAF mutated melanocytes with virtually no mitotic activity. This intradermal component with these unique features and the nearby conventional nevus-like lesion, to our minds, clearly sets these tumours apart from ASTs and other melanocytic lesions. Acknowledging that these lesions are characteristic of, although possibly not unique to, BAP1 mutation carriers, we proposed naming them melanocytic BAP1-mutated atypical intradermal tumours (MBAITs) 12.

3 Carbone et al. Page 3 Malignant tumours in BAP1 families Wiesner and colleagues noted that in the families that they studied there was a possible increased risk of cutaneous melanoma and UVM 10 and subsequently they confirmed the association with mesothelioma in 2 out of the 3 European families they studied 14. Such cancer clustering has been noted in other studies. A 1972 case report described an individual who developed UVM, mesothelioma and meningioma 15. Harbour et al. 7 reported inactivating mutations of BAP1 in 26 of 31 aggressive (class 2) UVMs, among which a germline mutation was found in 1 of 26 patients. Abdel-Rahman et al. 16 investigated BAP1 status in 53 unrelated patients with UVM who had a high risk of hereditary cancer. One of the 53 patients had a germline truncating BAP1 mutation that segregated in other family members. In this family, there were 7 BAP1 mutation carriers (5 tested and two inferred) and only one was cancer free at age 55; the other six had developed UVM (2 cases), cutaneous melanoma, mesothelioma, meningioma, lung and other types of carcinoma. The two patients treated for UVM subsequently developed a second malignancy 16. Njauw et al. 13 sequenced BAP1 from 50 patients with metastatic UVM and 50 patients with non-metastatic UVM. BAP1 was mutated in the germline of 8% of patients with metastatic UVM: none of the patients with non-metastatic UVM had BAP1 germline mutations. Also, they found that 2 of 7 probands from families with UVM-cutaneous melanoma carried germline BAP1 mutations, compared to 1 of 193 probands from families with a history of cutaneous melanoma and no UVMs (p=0.003). Aoude et al. 17 reported that 2 of 66 (3%) patients with UVM unselected for family history carried germline BAP1 mutations. Additional tumour types, including renal, breast and lung carcinomas were also found among BAP1 mutation carriers 6, 13, 16. Very recently, BAP1 mutations have been reported in sporadic renal carcinoma 18. Moreover, a novel inactivating germline splice mutation was reported in a family with UVM and cutaneous melanoma. Members of this family were also affected by multiple myeloma, paraganglioma and breast cancer 19. Table 1 summarizes the reports on families with BAP1 mutations as of January 17, To date, 53 of 76 BAP1 germline mutation carriers have developed malignancies; 13 of these 53 developed two or more cancers. Table 1 and our meta-analysis 12, indicate that mesothelioma, UVMs and cutaneous melanomas are clearly associated with the putative BAP1 cancer syndrome (Box 1). In addition, 35 BAP1 mutation carriers were investigated for the lesions that we call MBAITs, and 19 of them were found to have one or more (Table 1). These benign skin lesions may occasionally progress to cutaneous melanoma 14. In the BAP1 mutation families, there is an exceptionally high incidence of malignancy overall (69.74% cancer incidence among 76 mutation carriers). Some of these malignancies developed at an earlier age than observed in the general population (Table 1), as expected in familial cancer syndromes. All of the mutation carriers unaffected by cancer are younger than 55 years old and may develop malignancies later in life. However, the relatively high frequency in the general population of the carcinomas that develop in BAP1 mutation carriers does not allow us yet to distinguish with certainty if the association with carcinomas is causal. It should also be noted that population-based studies have shown that mutation carriers in the general population may have a lower risk than suggested by estimates obtained from families with multiple tumours, as for example observed for CDKN2A mutations 20. Therefore, large population based studies will be required to validate the association of germline BAP1 mutations with the exceptionally high incidence of malignancies detected in the BAP1 mutant families.

4 Carbone et al. Page 4 Most autosomal dominant cancer syndromes cause tumours in specific sites and tissues, and do not result in an overall increase in cancer risk. However, the Li-Fraumeni syndrome, caused by inherited mutations of the TP53 tumour suppressor gene, is an autosomal dominant cancer syndrome characteristically associated with the development of multiple tumour types 21. Is the BAP1 cancer syndrome, similarly to the Li-Fraumeni syndrome, associated with an overall general increase of cancer risk? If so, why do mesotheliomas, UVMs, cutaneous melanomas predominate? As we expand our studies to include additional families that have mutant BAP1 genes we will address these questions. In the section below we discuss the putative functions of BAP1 and how they might contribute to cancer. Most data about BAP1 function were published in the past 2 years, following a renewed interest in this gene caused by the discovery of its association with human mesothelioma and melanomas, and possibly with other cancer types. Therefore, some of the most recent studies have still to be independently verified. Overall the data indicate that BAP1 can influence multiple cellular pathways, at times suppressing and at times promoting cell growth, possibly in a cell type and species specific fashion. BAP1 has multifaceted functions Protein ubiquitylation was initially seen as a mechanism to label proteins for degradation; however, this notion has evolved as we have come to understand that ubiquitylation and DUBs regulate various cellular processes, including DNA repair, gene transcription, cell membrane trafficking, cell cycle progression, stress response, cell communication, differentiation and apoptosis, and that they have a role in cancer development 22, 23. The BAP1 gene is located at chromosome region 3p , a genomic region that is deleted in several human malignancies, including approximately 30-60% of mesotheliomas 6, 8, 9, 25 and 85% of metastasizing UVMs 10. BAP1 was discovered in a yeast two-hybrid screen owing to its interaction with the RING finger domain of the tumour suppressor BRCA1. Initial data suggested that BAP1 was a tumour suppressor gene because BAP1 suppressed the growth of MCF7 human breast cancer cells in soft agar 24. However, despite the fact that auto-ubiquitylated BRCA1 is a DUB substrate, subsequent experiments have shown that BAP1 does not influence the deubiquitylation of BRCA1 26. BRCA1 forms a heterodimer through its RING domain with BRCA1 associated RING domain 1 (BARD1), and this BRCA1 BARD1 tumour-suppressor complex has E3 ubiquitin ligase activity that regulates the DNA damage response (DDR) 27. BAP1 binds and deubiquitylates BARD1, thus modulating the E3 ligase activity of BRCA1 BARD1 28. Indeed, inhibition of BAP1 by short hairpin RNAs (shrnas) impaired the DDR and caused HeLa cells to become hypersensitive to ionizing radiation resulting in S-phase retardation, a phenotype similar to BRCA1 deficiency. Therefore, BRCA1-mediated ubiquitylation and BAP1-mediated deubiquitylation may coordinately regulate these cellular processes 28. Ventii et al. reported that in human NCI-H226 non-small cell lung cancer cells that do not express BAP1, its exogenous expression through lentivirus infection accelerated passage through the G1/S checkpoint. This effect might have impaired DNA repair leading to DNA damage and in the induction of cell death both by apoptosis and necrosis 29. Accordingly, NCI-H226 cells expressing exogenous wild-type BAP1 grew poorly in cell culture and when injected into athymic nude mice, they formed tumours that were about fold smaller than those obtained with cells infected with vector alone or expressing BAP1 mutants lacking deubiquitylating activity (C91A substitution) or the second nuclear localization signal, NLS2, that is required for the nuclear compartmentalization of BAP1 29. BAP1- mediated growth suppression in this in vivo model was BRCA1-independent. These data were interpreted as further evidence that BAP1 is a tumour suppressor gene, and that both

5 Carbone et al. Page 5 nuclear localization and deubiquitylating activity are required for BAP1-mediated tumour suppressor activity 29. When in the nucleus, BAP1 interacts with several proteins including host cell factor 1 (HCF1; also known as HCFC1). HCF1 is a cell cycle regulator that contains a Kelch domain that binds to a conserved peptide sequence known as the HCF1 binding motif (HBM) common to several transcription factors. Although HCF1 does not bind DNA directly, transcription factors that contain a HBM domain recruit HCF1 to specific promoters, where it regulates transcription. HCF1 binds BAP1 through a HBM sequence present in the middle portion of BAP1 30, 31. HCF1 is ubiquitylated on lysine residues, preferentially through K48- linked and K63-linked chains. BAP1 hydrolyzes K48-linked chains 30, 31. Overexpression of mutated, catalytically inactive BAP1 30 and BAP1 silencing 31 resulted in a modest accumulation of HCF1, indicating that HCF1 K48 ubiquitylation does not necessarily label HCF1 for degradation. A critical role of HCF1 is to sustain the formation of complexes between chromatin modifying enzymes and transcription factors. For example, by recruiting histone methyltransferases to the E2F1 transcription factor, HCF1 allows the transcription of E2F1 target genes, including genes that control the cell cycle and cellular proliferation 32. It has been proposed that ubiquitylation of HCF1 blocks E2F-responsive promoter activity, and that HCF1 deubiquitylation by BAP1 would remove this inhibition and promote cell proliferation 33. In addition, co-immunoprecipitation experiments have shown that BAP1 directly associates with E2F family members, suggesting a possible additional direct control of these transcriptional factors by BAP1 33. Therefore, the ability of BAP1 to influence progression through the cell cycle may be mediated, at least in part, by E2F family members 33. The Drosophila Polycomb group (PcG) gene calypso is homologous to the human BAP1 gene. PcG proteins are transcriptional repressors that control cellular differentiation and development 34. These proteins form multiprotein complexes that bind to chromatin and together repress gene expression. The two main families of PcG protein complexes are Polycomb repressive complex 1 (PRC1) and PRC2. Compaction of nucleosomes and ubiquitylation of histone 2A (H2A) are two of the mechanisms through which PRC1 silences gene expression 35. Mono-ubiquitylation of H2A is a critical mechanism in the control of transcription initiation and elongation, transcription silencing and DNA repair 36. In Drosophila, Calypso binds additional sex combs (ASX) to form the Polycomb repressive deubiquitinase (PR-DUB) complex that specifically removes mono-ubiquitin from H2A. Similarly, in humans BAP1 binds ASXL1 through its carboxy-terminus and deubiquitylates H2A 34. It appears possible that PR-DUB may help fine-tune gene expression levels by preventing the hyperubiquitylation of H2A by PRC1 and BRCA1. BRCA1 maintains the ubiquitylated status of H2A, a mechanism that is critical for BRCA1 tumour suppressor activity because it antagonizes the transcriptional de-repression of satellite DNA repeats that contribute to breast cancer growth 37. Thus, PcG activity may not simply be ascribed to its gene suppressive functions, but may rather result from the delicate balance between H2A ubiquitylation by PRC1 and BRCA1 (which suppress transcription) and H2A deubiquitylation by PR-DUB (which should reactivate transcription). PRC2 might also be important in tumours that have mutant BAP1. The PRC2 proteins EZH2 and EED are frequently overexpressed in mesothelioma and Kemp et al. proposed inhibiting PRC2 as a possible therapeutic strategy for treating mesothelioma 38.

6 Carbone et al. Page 6 Yu et al. 39 reported that almost all cellular BAP1 is found in high-molecular weight multiprotein complexes, which include HCF1, ASXL1 and ASXL2, O-linked N- acetylglucosamine transferase (OGT) and the forkhead transcription factors FOXK1 and FOXK2, in what they called the BAP1 core complex (Figure 1a). This core complex, in turn, associates with additional regulators and transcription factors to form specific functional complexes that may be cell type specific 39. In addition, recent studies have shown that BAP1, HCF-1 and OGT (which is essential for Polycomb mediate gene repression in Drosophila 40 ) exist as a multiprotein complex that regulates itself and also regulates several targets (Figure 1b). For example, BAP1 deubiquitylates and thus stabilizes OGT, which O- GlcNacylates HCF1 and activates it 41, 42. Activated HCF1 recruits OGT to O-GlcNacylate peroxisome proliferator activator receptor γcoactivator 1α(PGC-1α. This modification enables BAP1 to bind and deubiquitylate PGC-1αwhich is stabilized and can promote gluconeogenesis 42 (Figure 1b). Moreover, chromatin immunoprecipitation (ChIP) assays identified a total of 9128 BAP1 peaks (5926 located near the transcription start sites of 5731 genes); 85% of promoters occupied by BAP1 also contained HCF1, and BAP1, HCF-1 and OGT were recruited as a complex on 1827 different promoters 41. BAP1 has also been shown to form a ternary complex with HCF1 and the transcription factor Yin Yang 1 (YY1) that controls the transcription of genes involved in cell proliferation. Specifically, BAP1 interacts with the zinc fingers of YY1 through its coiledcoil motif and it is recruited together with HCF1 to the promoter of the COX7C gene that encodes a component of the mitochondrial respiratory chain 39. Thus, it can be expected that BAP1 influences a wide array of cellular functions, and consistent with this hypothesis, BAP1 depletion by RNA inhibition induced significant changes in the expression of many genes that control various cellular pathways 39. In summary, despite the findings implicating BAP1 in suppression of proliferation 24, 29, some groups have reported that BAP1 promotes cell proliferation 25, 30, 33. These conflicting results suggest that BAP1 effects may be cell-type specific and/or that they could be influenced by the experimental approach used. BAP1 activity could vary in different cells because the BAP1 core complex 39 is likely to associate with different transcription factors and thus influence different pathways in a cell-type specific manner. Also, some of the proteins that normally bind BAP1 may have different activities in its absence. For example, YY1 acts as a repressor or an activator of COX7C depending on whether it is bound to the BAP1 HCF1 complex 39. All the germline mutations found in the BAP1 cancer syndrome encode BAP1 proteins lacking the nuclear localization sequence and most of them have an intact UCH domain (Figure 2). In the tumours that develop in BAP1 mutant carriers, BAP1 expression is either absent because of loss of heterozygosity (LOH), resulting in biallelic inactivation, or the BAP1 protein is localized in the cytoplasm 6, 16 where it may retain DUB activity, with as yet unknown possible effects on the cellular ubiquitinome. What do BAP1 associated cancers have in common? Because germline BAP1 mutations are associated with mesotheliomas, UVMs and cutaneous melanomas, there should be some common pathways controlled by BAP1 that are of particular importance to the development of these malignancies. The finding that somatic BAP1 mutations are common in these cancers supports this interpretation. Somatic BAP1 mutations were found in 84% of metastasizing UVM biopsies 7 and in 22% 6 and 23% 25 of sporadic US mesothelioma biopsies. Instead, Yoshikawa et al. found somatic inactivating BAP1 mutations in 61% of Japanese mesothelioma biopsies 43. To address this

7 Carbone et al. Page 7 discrepancy, in collaboration with our Japanese colleagues we are currently investigating whether the higher incidence of BAP1 mutations that they detected was caused by ethnic differences or by the different technical approach used, that is multiplex ligation-dependent probe amplification (MLPA) 43 compared with PCR 6. Studies in mice 41 revealed that Bap1 deletion is lethal during embryogenesis. When Bap1 was deleted in adult mice that expressed the tamoxifen-inducible recombinase cre ERT2 ubiquitously and had Bap1 exons 4 and 5 flanked by lox sites, these mice developed splenomegaly caused by extramedullary hematopoiesis, monocytosis, neutrophilia, and progressive anemia 41, findings comparable to the human myelodysplastic syndrome (MDS). The absence of mesothelioma, uveal and cutaneous melanoma in these mice is somewhat puzzling, and may be related to species differences. In addition, at the level of disease causation, the common denominator among mesotheliomas and melanomas is a well-defined role of environmental carcinogens. We are presently testing the hypothesis that germline Bap1 mutations increase the susceptibility to mineral fibre-induced and UV light-induced carcinogenesis; this may explain the absence of mesothelioma and melanoma in mice that were not exposed to these carcinogens. Mineral fibres are well-documented causes of mesothelioma 1-5. Deposition of these fibres in tissues induces a chronic inflammatory reaction that is started by the release of high mobility group box 1 (HMGB1) by mesothelial cells undergoing programmed cell necrosis 2, 44. Extracellular HMGB1 starts the inflammatory process by recruiting macrophages and other immune cells that in turn actively secrete HMGB1 and other cytokines, and release mutagenic oxygen and nitric radicals, promoting carcinogenesis and mesothelioma growth (reviewed in 2 ). In such an environment, an impaired DDR caused by BAP1 deletion, might be expected to favour the accumulation of genetic damage that eventually gives rise to a malignant clone. However, Bott et al. 25 found no consistent differences in RAD51 or BRCA1 complex formation between BAP1 wild-type and BAP1 deficient cell lines exposed to ionizing radiation, suggesting no critical role for BAP1 in the formation of these DNA repair complexes. Moreover, we 6 found very minimal and Wiesner et al 14, found no evidence of exposure to asbestos or erionite among the patients who developed mesothelioma in the families with BAP1 mutations, findings that do not support gene-environment interaction in causing mesothelioma, at least in these families. A separate mechanism, by which HMGB1 might influence mesothelioma especially in cells lacking BAP1, is the capacity of HMGB1 to induce nucleosome assembly 45. High levels of HMGB1 may increase gene repression by PRC1 in a mutated BAP1 background. Ultraviolet (UV) light exposure increases the risk of cutaneous melanoma. The role of UV light exposure in causing UVM is still debated 46 however, it seems possible that BAP1 mutation carriers are at an increased risk because of impaired DDR. Because BAP1 mutation carriers represent only a fraction of patients who develop UVM (3-4%) 13, 17, the overall association of UVM with UV light exposure should be re-evaluated in BAP1 mutations positive versus BAP1 mutation negative patients. Moreover, BAP1 is a UV-inducible substrate of the ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related (ATR) kinases. ATM and ATR regulate the DNA damage checkpoint that inhibits cell cycle progression in response to genomic insults such as UV to allow time to damage repair or, if the damage cannot be repaired, to enter apoptosis 47. We anticipate that as more studies uncover the precise mechanisms by which BAP1 mutations cause mesothelioma and melanomas, specific preventive and therapeutic approaches for these malignancies will be developed (Box 1). It is worth noting that a recent paper showed that histone deacetylase inhibitors (HDACIs) impair the in vitro and in vivo

8 Carbone et al. Page 8 Conclusions Acknowledgments Glossary References growth of UVM metastatic tumours with BAP1 inactivating mutations 48. Specifically, HDAC inhibitors block H2A ubiquitylation through inhibition of PRC1 and so reverse H2A hyperubiquitylation caused by BAP1 loss. HDACIs also induce differentiation of Class II UVM to Class I UVM 48, which is less aggressive. The apparent ability of BAP1 mutations to cause multiple tumour types and the high tumour phenotype penetrance (Table 1) suggests that this gene has a major role in influencing cancer cell growth. The pleiotropic effects of BAP1 can account for this finding. Not only is BAP1 the human homologue of the Drosophila PcG gene calypso, but it also contains a HBM domain, which is absent in Calypso, that allows BAP1 to bind HCF1, a chromatin associated protein that similar to PcG regulates the expression of a plethora of different genes. Thus, by regulating the activities of PcG and HCF1 target genes, BAP1 has a central role in regulating gene expression in mammalian cells. The clinical implications of these recent findings have yet to be fully established 12. The presence of specific skin lesions that we have called MBAITs is an important clinical feature that might help to identify BAP1 mutation carriers. We think that these patients require close monitoring for early detection and curative resection of uveal and cutaneous melanoma, and of mesothelioma and other malignancies. We thank Drs. Francine Baumann, Erin Flores, Shreya Kanodia, Andrea Napolitano, Janet D. Rowley and David C. Ward for critical reading of this manuscript. We thank Dr. T. Wiesner for sharing with us MBAITs tissue specimens for our review. NCI PO1 CA to MC supported this work. Uveal melanoma [UVM] Proband Autosomal dominant Uveal melanomas are the most common primary intraocular malignancy and they account for about 13% of melanoma deaths. Individual that, by seeking medical/scientific attention, allows the detection of a genetic disorder in a family. A genetic disease inherited as a result of having a single copy of the mutated gene, located on one of the 22 non-sex chromosomes. Asbestos This term identifies 6 different fibrous minerals among about 400 present in nature that were used commercially. Exposure to asbestos as well as to other mineral fibres, such as erionite, can cause mesothelioma. 1. Carbone M, et al. A mesothelioma epidemic in Cappadocia: scientific developments and unexpected social outcomes. Nat Rev Cancer. 2007; 7: [PubMed: ] 2. Carbone M, Yang H. Molecular pathways: targeting mechanisms of asbestos and erionite carcinogenesis in mesothelioma. Clin Cancer Res. 2012; 18: [PubMed: ] 3. Carbone M, et al. Erionite exposure in North Dakota and Turkish villages with mesothelioma. Proc Natl Acad Sci U S A. 2011; 108: [PubMed: ]

11 Carbone et al. Page 11 Box 1 Clinical considerations Wiesner et al 10 noted that in BAP1 mutation carriers benign melanocytic tumors develop during the second decade of life and their number increases with age, a finding confirmed in the BAP1 mutant families we are studying 12. The occurrence of cutaneous melanoma in some BAP1 mutation carriers, including one that appeared to originate from these melanoctytic tumors 14, suggests that occasionally these benign tumors may undergo malignant transformation. This finding may account, at least in part, for the overall high risk of cutaneous melanoma detected among BAP1 germline mutation carriers (Table 1). We propose that these melanocytic tumors that for the reasons described in the text and in ref. 12 we term MBAITs, could be viewed as a specific phenotypic marker of BAP1 mutation carriers. Other autosomal dominant cancer syndromes also have phenotypic markers, such as adenomas in familial adenomatous polyposis families and Lisch nodules and café-au-lait spots in families with neurofibromatosis type 1. Suspected MBAITs could be removed and tested to identify patients with BAP1 mutations. If this approach was found to have clinical relevance it could allow for the close monitoring of these individuals and enable early detection of cutaneous melanoma, uveal melanoma (UVM), mesothelioma and possibly other cancers 12. Both cutaneous melanoma and UVM can be cured when detected at an early stage and are fatal when they have metastasized. Early diagnosis also benefits mesothelioma patients because when they are diagnosed and treated at a very early stage, survival for 5 or more years is not uncommon 12, 49. Thus, the detection of families with inherited mutations of BAP1 offers opportunities for early cancer detection and more effective therapeutic approaches.

Contents Introduction: biology and medicine, two separated compartments What we need to know: - boring basics in DNA/RNA structure and overview of particular aspects of molecular biology techniques - How

How many of you have checked out the web site on protein-dna interactions? Example of an approximately 40,000 probe spotted oligo microarray with enlarged inset to show detail. Find and be ready to discuss

Open Access: Full open access to this and thousands of other papers at http://www.la-press.com. Clinical Medicine Insights: Oncology The Importance of Multidisciplinary Approach in Early Detection of BAP1

LESSON 3.5 WORKBOOK How do cancer cells evolve? In this unit we have learned how normal cells can be transformed so that they stop behaving as part of a tissue community and become unresponsive to regulation.

MUTATION, DNA REPAIR AND CANCER 1 Mutation A heritable change in the genetic material Essential to the continuity of life Source of variation for natural selection New mutations are more likely to be harmful

Mesothelioma 1. Introduction 1.1 General Information and Aetiology Mesotheliomas are tumours that arise from the mesothelial cells of the pleura, peritoneum, pericardium or tunica vaginalis [1]. Most are

Histone modifications and ChIP Histone acetylation It has been shown that the acetylation of lysines is highly dynamic and regulated by the opposing action of two families of enzymes, histone acetyltransferases

1. Which of the following is NOT involved in binary fission in prokaryotes? a. Replication of DNA b. Elongation of the cell c. Separation of daughter cells by septum formation d. Assembly of the nuclear

Non Small Cell Lung Cancer: Scientific Discoveries and the Pursuit of Progress Lung Cancer Accounts for 14% of All New Cancer Diagnoses in the United States 1 Lung cancer is the second most common malignancy

Male Female Death rates from lung cancer in USA Smoking represents an interesting combination of an entrenched industry and a clearly drug-induced cancer Tobacco Use in the US, 1900-2000 5000 100 Per Capita

CHAPTER 2: UNDERSTANDING CANCER INTRODUCTION We are witnessing an era of great discovery in the field of cancer research. New insights into the causes and development of cancer are emerging. These discoveries

Hereditary Ovarian cancer: BRCA1 and BRCA2 Karen H. Lu MD September 22, 2013 Outline Hereditary Breast and Ovarian Cancer (HBOC) BRCA1/2 genes How to identify What it means to you What it means to your

Special report Chronic Lymphocytic Leukemia (CLL) Genomic Biology 3020 April 20, 2006 Gene And Protein The gene that causes the mutation is CCND1 and the protein NP_444284 The mutation deals with the cell

Lesson 3 Reading Material: Oncogenes and Tumor Suppressor Genes Becoming a cancer cell isn t easy One of the fundamental molecular characteristics of cancer is that it does not develop all at once, but

The RNA strategy RNA as a tool and target in human disease diagnosis and therapy. The Laboratory of RNA Biology and Biotechnology at the Centre for Integrative Biology (CIBIO) of the University of Trento,

Gene mutation and molecular medicine Chapter 15 Lecture Objectives What Are Mutations? How Are DNA Molecules and Mutations Analyzed? How Do Defective Proteins Lead to Diseases? What DNA Changes Lead to

Mutations Mutations: 2 general ways to alter DNA Change a single DNA base Or entire sections of DNA can move from one place to another What is a mutation? Any change in the nucleotide sequence of DNA Here

The world of non-coding RNA Espen Enerly ncrna in general Different groups Small RNAs Outline mirnas and sirnas Speculations Common for all ncrna Per def.: never translated Not spurious transcripts Always/often

Molecular mechanisms of carcinogenesis Nik Hodges School of Biosciences N.Hodges@bham.ac.uk Aims What is cancer at the cellular level? How do chemical and physical agents cause cancer? How do we test for

DISCUSSION CHAPTER 4 4.1 3T12 and 312 are immortalized cell lines with transforming potential: Transformation of a normal cell with finite number of divisions into a tumorigenic cell of potentially infinite

GENETIC TESTING FOR INHERITED MUTATIONS OR SUSCEPTIBILITY TO CANCER OR OTHER CONDITIONS MED207.110 COVERAGE: Pre- and post-genetic test counseling may be eligible for coverage in addition to the genetic

PROTOCOL OF THE RITA DATA QUALITY STUDY INTRODUCTION The RITA project is aimed at estimating the burden of rare malignant tumours in Italy using the population based cancer registries (CRs) data. One of

Lecture 3: Mutations Recall that the flow of information within a cell involves the transcription of DNA to mrna and the translation of mrna to protein. Recall also, that the flow of information between

Griffith University - Case for Support Mesothelioma Research Program Professor Lyn Griffiths Director, Dean, Research and Director, Genomics Research Centre (GHI) Established in 2007. Integrated health

THE EUKARYOTIC CELL CYCLE AND CANCER: IN DEPTH ABOUT THIS WORKSHEET This worksheet complements the Click and Learn The Eukaryotic Cell Cycle and Cancer and is intended as an in-depth examination of the

Where does Molecular Analysis of FNA Specimens fit into the evaluation of thyroid nodules? The Role of Genetic Testing in the Evaluation of Thyroid Nodules Ultrasound TSH Risk factors Jill E. Langer, MD

AP * BIOLOGY GENE REGULATION Teacher Packet AP* is a trademark of the College Entrance Examination Board. The College Entrance Examination Board was not involved in the production of this material. Pictures

The Genetics of Early- Onset Breast Cancer Cecelia Bellcross, Ph.D., M.S.,C.G.C. Department of Human Genetics Emory University School of Medicine All cancers are genetic BUT Not all cancers are hereditary

# Circuits relevant to cancer, related to known TFs. # Final dataset of circuits where at least one gene has been annotated as relevant to cancer. # Circuits correspond to blocks separated by a symbol.

Major Advances in Cancer Prevention, Diagnosis and Treatment~ Why Mesothelioma Leads the Way H. Richard Alexander, Jr., M.D. Department of Surgery and The Greenebaum Cancer Center University of Maryland

For media outside the US, UK and Canada only P L E U R A L M E S O T H E L I O M A 1. Overview 2. What is pleural mesothelioma? 3. How common is pleural mesothelioma? 4. What are the risk factors for pleural

Update in Hematology Oncology Targeted Therapies Mark Holguin 25 years ago Why I chose oncology People How to help people with possibly the most difficult thing they may have to deal with Science Turning

Fulfilling the Promise Advancing the Fight Against Cancer: America s Medical Schools and Teaching Hospitals For more than a century, the nation s medical schools and teaching hospitals have worked to understand,

Important information for people with advanced non-small cell lung cancer The following information is only meant for people who have been diagnosed with advanced non-small cell lung cancer (NSCLC). NSCLC

Cytology of Pleural Fluid as a Diagnostic Challenge Paavo Pääkkö,, MD, PhD Chief Physician and Head of the Department Department of Pathology, Oulu University Hospital,, Finland Oulu University Hospital

Cancer Cancer is one of the most common diseases in the developed world: 1 in 4 deaths are due to cancer 1 in 17 deaths are due to lung cancer Lung cancer is the most common cancer in men Breast cancer