Services provided by the BioMicro Center are billed monthly to MIT account numbers (7-digit numbers). If you do not have an MIT account number, you will need to provide a PO number for our services. Cost estimates are available by request.

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== [[BioMicroCenter:Sequencing|ILLUMINA SEQUENCING]] ==

== [[BioMicroCenter:Sequencing|ILLUMINA SEQUENCING]] ==

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=== SEQUENCING ===

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the Genome Analyzer.

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the Genome Analyzer.

| colspan="5" align=center |''GAII Services are now completely a la carte to allow us to run partial lanes. No guarantees are made on partial flowcell runs. Price includes data storage for two years but does not include quality control. PhiX lanes must be included in your purchased lanes if you want to use control normalization.''

| Uses 6nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.

+

| Uses 6-8nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.

|-

|-

| Quality Control Only

| Quality Control Only

-

|align="right"| $40

+

|align="right"| $30

-

|align="right"| $60

+

|align="right"| $45

|align="center"| per sample

|align="center"| per sample

-

|

+

| Includes RT-PCR and BioAnalyzer

|}

|}

<BR>

<BR>

<BR>

<BR>

-

Prices effective 7.1.2010 <BR>

+

Prices effective 7.1.2012 <BR><BR>

+

Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the Genome Analyzer. The BioMicro Center reserves the right to reject samples for sample preparations.

* available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.

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-

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-

^ Discount pricing available through the BioMicro Center.

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== [[BioMicroCenter:2100BioAnalyzer|AGILENT BIOANALYZER]] ==

== [[BioMicroCenter:2100BioAnalyzer|AGILENT BIOANALYZER]] ==

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! AGILENT BIOANALYZER

! AGILENT BIOANALYZER

!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]] / MIT

!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]] / MIT

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!width=100| Non-MIT

+

!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]

!width=100| unit

!width=100| unit

! Notes

! Notes

|-

|-

-

| All DNA and RNA samples

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| High Sensitivity DNA or Nano RNA

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|align="right"| $10

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|align="right"| $25

+

|align="center"| per sample

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|

+

|-

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| All other DNA and RNA samples

|align="right"| $15

|align="right"| $15

|align="right"| $25

|align="right"| $25

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|-

|-

|}

|}

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prices effective 7.1.2010

+

prices effective 7.1.2012

<br>.

<br>.

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! OTHER LAB SERVICES

! OTHER LAB SERVICES

!width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]

!width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]

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!width=100| Non-MIT

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!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]

!width=100| unit

!width=100| unit

! SIGN UP

! SIGN UP

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|-

|-

| Fluidigm BioMark

| Fluidigm BioMark

-

|align="right"| $100

|align="right"| $150

|align="right"| $150

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|align="right"| $225

+

|align="center"| per use

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| [[BioMicroCenter:Forms#Calenders|CALENDER]]

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| Contact [[BioMicroCenter:People|Shmulik]]

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|-

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| 48.48 Dynamic Array Chip

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|align="right"| $260

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|align="right"| N/A

+

|align="center"| per cassette

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| Cassettes are available in the BMC.

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| Contact [[BioMicroCenter:People|Shmulik]]

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|-

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| 96.96 Dynamic Array Chip

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|align="right"| $760

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|align="right"| N/A

|align="center"| per cassette

|align="center"| per cassette

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| Cassettes will be available in BMC soon.

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| Cassettes are available in the BMC.

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| Contact [[BioMicroCenter:People|Manlin]]

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| Contact [[BioMicroCenter:People|Shmulik]]

|-

|-

| colspan="6" align="center"| '''RT-PCR'''

| colspan="6" align="center"| '''RT-PCR'''

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|align="center"| per plate

|align="center"| per plate

| [[BioMicroCenter:Forms#Calenders|CALENDER]] *available for CORE only

| [[BioMicroCenter:Forms#Calenders|CALENDER]] *available for CORE only

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| Contact [[BioMicroCenter:People|Allison]] for training

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| Contact [[BioMicroCenter:People|Michael]] for training

|-

|-

| MJ Opticon II

| MJ Opticon II

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| Contact [[BioMicroCenter:People|Manlin]] for training

| Contact [[BioMicroCenter:People|Manlin]] for training

|-

|-

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| SYBR Green (Roche)

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| SYBR Green (KAPA/Roche)

-

|align="right"| $40

+

|align="right"| $34

|align="right"| NA

|align="right"| NA

|align="center"| per ml

|align="center"| per ml

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|-

|-

|}

|}

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prices effective 7.1.2010

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prices effective 7.1.2012

.

.

<br>

<br>

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! OTHER LAB SERVICES

! OTHER LAB SERVICES

!width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]

!width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]

-

!width=100| Non-MIT

+

!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]

!width=100| unit

!width=100| unit

! SIGN UP

! SIGN UP

! Notes

! Notes

|-

|-

-

| colspan="6" align="center"| '''PLATE READER'''

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| colspan="6" align="center"| '''PIPPIN PREP'''

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|-

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|Gel Isolation

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|align="right"| $50

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|align="right"| $75

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|align="center"| per gel (5 lanes)

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| TBA

+

|

+

|-

+

|Training

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|align="right"| $25

+

|align="right"| $50

+

|align="center"| per session

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| TBA

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| Contact [[BioMicroCenter:People|Shmulik]] for training.

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|-

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| colspan="6" align="center"| '''PLATE READER'''

|-

|-

| Varioskan

| Varioskan

|align="right"| $10

|align="right"| $10

|align="right"| $15

|align="right"| $15

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|align="center"| per plate

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|align="center"| per session

| [[BioMicroCenter:Forms#Calenders|CALENDER]]

| [[BioMicroCenter:Forms#Calenders|CALENDER]]

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| Quick scans (those requiring less then 30 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact [[BioMicroCenter:People|Kevin]] for training

+

| Quick scans (those requiring less then 30 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact [[BioMicroCenter:People|Shmulik]] for training

Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 yrs.

Paired End (40+40nt)

$1,850

$2,775

per lane

Add'l 40nt

$370

$555

per lane

MiSeq sequencing (minimum 2m reads/lane. Average ~5m reads/lane)

50nt Run

$1,000

$1,500

per lane

Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 years

300nt Run (PE or SE)

$1,300

$1,950

per lane

Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 years

500nt Run (PE or SE)

$1,450

$2,175

per lane

Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of fastq files for 2 years

GAII / GAIIx sequencing (Average ~25-40m reads/lane)

GAII Services are now completely a la carte to allow us to run partial lanes. No guarantees are made on partial flowcell runs. Price includes data storage for two years but does not include quality control. PhiX lanes must be included in your purchased lanes if you want to use control normalization.

8 Lanes (SE/PE)

$4,835 / $7,135

$7,253 / $10,702

1 day

24nt per day. 42nt per seq kit.

4 Lanes (SE/PE)

$3,465 / $4,700

$5,198 / $7,050

1 day

36nt per day. 72nt per seq kit.

2 Lanes (SE/PE)

$2,780 / $4,015

$4,170 / $6,023

1 day

48nt per day. 120nt per seq kit.

1 Lane (SE/PE)

$2,440 / $3,675

$3,660 / $5,513

1 day

72nt per day. 180nt per seq kit.

Add'l Day

$325

$488

1 day

covers service contract and data storage requirements.

Add'l Sequencing Kit

$1,400

$2,100

1 Kit

Additional Services

These services apply to samples submitted for all platforms.

Sample Multiplexing

$50

$75

per 10 samples

Combined over the whole order. Only applies to samples combined by BMC technicians.

Add'l Repriming

$200

$300

per flow cell

Uses 6-8nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.

Quality Control Only

$30

$45

per sample

Includes RT-PCR and BioAnalyzer

Prices effective 7.1.2012

Please note that samples submitted from CORE LABs will be given priority on the Genome Analyzer. The BioMicro Center reserves the right to reject samples for sample preparations.