Avi-tag BiotinylatedE. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Related Products

Target Data

Function

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.

Results show an association of PD with p53 and active caspase-3 overexpression and beta-adrenergic receptor underexpression in the heart, potentially promoting the cardiac autonomic dysfunction frequently observed in PD. PMID: 30135418

Because DFNA5-induced secondary necrosis and GSDMD-induced pyroptosis are dependent on CASP3 activation, we propose that they are forms of programmed necrosis. PMID: 28045099

Results revealed an interaction between Oocyte-G1 and Caspase-3. Expression levels of Caspase-3 were upregulated in cells overexpressing Oocyte-G1 and downregulated in Oocyte-G1 knockdown cells and suggest that casp3 is activated by oocyte-G1 promoting male germ cell apoptosis. PMID: 29802994

NKG2D is upregulated, and ROS and caspase-3 are inhibited by Strongylocentrotus nudus egg polysaccharide-enhanced antitumor 5-FU in mice PMID: 27385218

findings suggest that caspase-3 activation can trigger necrosis by cleaving GSDME and offer new insights into cancer chemotherapy PMID: 28459430

Caspases and their substrates are key mediators of apoptosis. PMID: 27552481

Induction of apoptosis in epithelial cancer cells was accompanied by a switch from glycolysis to oxidative phosphorylation, cytosol acidification and caspase-3 activation. PMID: 28063999

This provides new insight into the immune phenotype, mechanisms, and signaling pathways that operate in microglial neurotoxic activation in amyotrophic lateral sclerosis. PMID: 28336525

ULK1 is a novel substrate of Caspase-3 and upregulation of ULK1 drives autophagy initiation in leukemia cells. PMID: 28381396

These results indicate that the caspase-3/p120 RasGAP stress-sensing module impacts on carcinogen-induced liver cancer incidence but not sufficiently so as to affect overall survival. PMID: 28150874

report that mutating the caspase-3 consensus site in the EAAT2 sequence with an aspartate to asparagine mutation PMID: 28342750

melatonin could inhibit TLR4 expression in hypoxic microglia followed by suppression of caspase3 activation leading to decrease in production of proinflammatory mediators PMID: 27812200

A significant emergence of GFP-expressing PCs and interneurons in symptomatic, but not non-symptomatic, SCA1 mice 2 weeks after the MSC injection. PMID: 27802273

Conversely, treatment with LY294002 (a selective inhibitor of Akt1) reversed the effects of quercetin. In conclusion, these findings highlight the important role of quercetin in protecting against cognitive deficits and inhibiting neuronal apoptosis via the Akt signaling pathway. We believe that quercetin might prove to be a useful therapeutic component in treating cerebral I/R diseases in the near future. PMID: 27450812

caspase-3 is essential for a subset of social behaviors, but despite similar hyper-locomotion in both sexes, only male Casp3(-/-) mice exhibited social interaction deficits, which is interesting given the male bias of autism PMID: 26783106

Study identified CASP-3 mRNA as a new miRNA-155 target in lipopolysaccharides-activated macrophages suggesting that through destabilization of CASP-3, miR-155 contributes to the protective role to sustain macrophage function in inflammation response. PMID: 26574931

does not significantly contribute to cardiomyocyte death induced by transient coronary occlusion PMID: 26924441

expression level of miR-98, which can regulate Caspase-3, was significantly decreased. Huwe1, the host gene of miR-98, was positively associated with miR-98 expression after Silica NP exposure PMID: 26263183

GSAP cleavage via caspase-3 is regulated and depend upon the availability of 5-Lipoxygenase in Alzheimer's disease. PMID: 26076991

The study demonstrates sublethal caspase-activation has an important role in cardiomyocyte differentiation and may have significant implications for promoting cardiac regeneration after myocardial injury involving exogenous or endogenous cell sources. PMID: 25763592

The hepatic expression level of cleaved caspase-3 protein in the group treated concomitantly with doxorubicin and theanine was significantly lower than that in the doxorubicin treated group. PMID: 25680506

Results show that human or mouse cells exposed to ionizing radiation or other stresses can survive with persistent caspase-3 activation, which in turn promotes genetic instability and oncogenic transformation. PMID: 25866249

Data indicate that procaspase-3 has a non-apoptotic function; it regulates the secretion of fibronectin and influences morphology, adhesion and migration. PMID: 24610949

Among 46 putative substrates identified in MN9D neuronal cells, we further evaluated whether caspase-3-mediated cleavage of anamorsin, a recently recognized cell death-defying factor in hematopoiesis, is a general feature of apoptosis. PMID: 24973211

Effect of cortisol on caspases in the co-cultured C2C12 and 3 T3-L1 cells. PMID: 24752940

these findings support the existence of a microRNA-21-responsive PDCD4/caspase-3 pathway in the pulmonary tissues that when active serves to promote endothelial apoptosis in vitro and pulmonary hypertension in vivo. PMID: 24732886

OCT-P407 induces mRNA expression of SSTR-2 and caspase-3 and decreases that of VEGF in mice. PMID: 24173662

These findings support a prominent role for hepatocyte caspase 3 activation in NASH-related apoptosis, fibrogenesis and fibrosis which in part is mediated via CCR2-dependent infiltration of Ly6c positive monocytes. PMID: 24795036

Kainic acid induced neurodegeneration and aroused several caspase 3-mediated molecular and cellular changes such as dendritic plasticity, neurogenesis, and gliosis. Acute caspase 3 activation occurred in pyramidal neurons as well as in hilar interneurons. PMID: 24313976

Which was significantly attenuated in the presence of the caspase-3 inhibitor. PMID: 24362325

these results support a causative role of VEGF in perinatal cerebral hemorrhage and implicate its downstream proteases as potential therapeutic targets. PMID: 23843451

HuR undergoes cleavage modification by caspase-3 following IR-induced oral mucositis and subsequently promotes the expression of the pro-apoptotic factor BAX (Bcl-2-associated X protein), as well as cell death. PMID: 24362034

Caspase-3 knock-out mice have increased spine density and altered miniature EPSCs, confirming a physiological involvement of caspase-3 in the regulation of spines in vivo. PMID: 24478350

The activity of caspase-3, -8 and -9 was significantly increased in the isoflurane-treated cells compared with the untreated cells. PMID: 23922164

The finding that BMMCs secrete caspase-3 and gzmB after Ag stimulation suggests that both proteases contribute to extracellular MC-mediated proteolytic events. PMID: 24414824

After activation by caspase-9, caspase-3 inhibits ROS production and is required for efficient execution of apoptosis, while effector caspase-7 is required for apoptotic cell detachment. PMID: 23834359

the presence of active caspase-3-like activity in granule-like compartments close to the plasma membrane was demonstrated. Moreover, it was shown that mast cell activation caused release of the caspase-3 to the cell exterior. PMID: 23817418

exposure to the EMF of 1950-MHz TD-SCDMA may not promote the tumor formation, but continuous exposure damaged the mitochondria of astrocytes and induce apoptosis through a caspase-3-dependent pathway with the involvement of bax and bcl-2. PMID: 22870319

TNF-alpha-induced apoptosis and Caspase-3 expression in mouse skin keratinocytes in vivo and in vitro PMID: 22585037

Caspase-3 is a stress intensity sensor that controls cell fate by either initiating a RasGAP cleavage-dependent cell resistance program or a cell suicide response mediated by akt. PMID: 22949508

Levels of caspase-3 activity, differ among alphabeta and gammadelta effector T-cell subsets and the degree of redistribution of active caspase from the cell membrane to the cytoplasm upon TCR restimulation is proportional to the susceptibility to cell death. PMID: 22117649