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Description

Preparation Method

Reagents are tested and chosen for their ability to promote optimum growth of yeast strains when combined in the DOB or DOBA with CSM-Met formula. DOB (Drop Out Base) is 1.7g Yeast Nitrogen Base, 5g Ammonium Sulfate, 20g Dextrose per liter. DOBA is DOB with 17% agar. Chosen reagents are combined in the proper proportions according to the formulation listed in the certificate of analysis and milled to a powder which thoroughly blends the reagents for uniform distribution. A sample of the milled and blended formulation was used to prepare liquid plate medium by adding 43.7g DOBA powder and 0.77g of CSM-Met to a liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve the dextrose and autoclaved at 121 °C for 25 minutes. After cooling to 50 °C plates were poured.

Applications

CSM-MET is a dropout supplement for virtually all strains of S. cerevisiae and allows to select for auxotrophic requirements and transformants. Cells grow vigorously in CSM-MET supplemented medium which is easily made by adding the CSM-MET powder to DOB of DOBA Base Medium and autoclaving.

Product Overview

Complete Supplement Mixture (CSM)-MET is a time-tested dropout supplement for virtually all strains of S. cerevisiae. Cells grow vigorously in CSM supplemented medium which is easily made by adding the CSM powder to DOB of DOBA Base Medium and autoclaving.

Product Description

CSM-MET is a Single dropout formulation of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media.

Recommended Use

Add 0.77g of CSM-Met to each liter of DOB or DOBA and autoclave at 121°C for 25 minutes for complete synthetic defined single drop-out medium for S.cerevisiae.