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Glucose, a monosaccharide, is the most important carbohydrate in biology. It is a source of energy and metabolic intermediate for cell growth. Glucose is one of the main products of photosynthesis and starts cellular respiration in both prokaryotes and eukaryotes. Glucose level is a key diagnostic parameter for many metabolic disorders. This glucose assay kit provides a quick and sensitive method for the measurement of glucose in various biological samples (e.g., serum, plasma, body fluid, food, growth medium, etc.). The kit uses our Amplite™ HRP substrate that making the kit recordable in a dual more, either fluorimetric (Ex/Em = 570nm/590 nm) or colorimetric readout (570 nm). The kit provides all the essential components with an optimized assay protocol. The assay is robust, and can be readily adapted for high-throughput assays in a wide variety of applications that require the measurement of glucose. For example, the assay might be suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes. It might also be used for monitoring glucose transporters.