It is recommended to reconstitute the lyophilized recombinant pituitary human growth hormone 20K in 0.4% NaHCO3 adjusted to pH 10, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized recombinant pituitary human growth hormone
20K although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution and filter sterilization recombinant pituitary human growth hormone 20Kcan be stored at 4C, pH 10 for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Protein quantitation of recombinant pituitary human growth hormone 20Kwas carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.74 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by DNAman computer analysis program of protein sequences.

Usage:

The recombinant pituitary human growth hormone 20 K is offered by PLR for research, laboratory or further manufacturing purposes.