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OK, here's the deal. About a month ago, I made up 22 half-pint jars and PCed them at 15psi for 60 minutes. Two weeks went by, no contams, so I inocced. The jars are 90-95% colonized. However, 15 of them show small amounts of green in the uncolonized parts. That sucks, I sterilized the room and sterilized the needle between jars. My syringes came from a reputable shroomery sponsor. Should I wait to see if the mycelium takes over or just keep the 7 that show no signs of contamination? What could possibly be going wrong? Could waiting too long after PCing to inoculate be my problem? Thanks.

Quote:Vagrant said:OK, here's the deal. About a month ago, I made up 22 half-pint jars and PCed them at 15psi for 60 minutes. Two weeks went by, no contams, so I inocced. The jars are 90-95% colonized. However, 15 of them show small amounts of green in the uncolonized parts. That sucks, I sterilized the room and sterilized the needle between jars. My syringes came from a reputable shroomery sponsor. Should I wait to see if the mycelium takes over or just keep the 7 that show no signs of contamination? What could possibly be going wrong? Could waiting too long after PCing to inoculate be my problem? Thanks.

I read on fanaticus.com that you could test the jars by letting them sit before inoculation to see if you are PCing correctly. I'm not going to do that again. Do yo think that could've been my problem?

I think on my next run, I'll omit the observation stage and inoculate right after cooling. I'm also making a tub in tub incubator that should be ready. Hopefully the myc will outgrow any contams.So tell me, should I throw out the jars that show a small amount of green or see if the myc out competes the contams? They are about 90-95% colonized.