This chapter talks about the mapping data for B. megaterium and compares these data with genetic mapping data compiled for B. amyloliquefaciens, B. licheniformis, B. stearothermophilus, B. subtilis, and B. thuringiensis. Considerable genetic mapping data have now been reported for several other bacilli. These data are presented, some in abbreviated form, in this chapter. In B. megaterium, the leu and ilv genes are inverted in relation to phe, the hemA gene is near metC and not near leu, argO and hem are inverted, and cysB and cysC are in the ribosomal protein region. The chapter contains a table providing details on genetic markers of B. megaterium.

Genetic map of B. megaterium. Shown are the six linkage groups A through F. The map is depicted as circular by analogy with B. subtilis, B. stearothermophilus. and B. cereus. Restriction fragments from NotI (N) or SfiI (S) digestion, with their corresponding size in kilobases, are also shown (37). Figure is from Vary (60).

10.1128/9781555818388/fig32-1_thmb.gif

10.1128/9781555818388/fig32-1.gif

Figure 1

Genetic map of B. megaterium. Shown are the six linkage groups A through F. The map is depicted as circular by analogy with B. subtilis, B. stearothermophilus. and B. cereus. Restriction fragments from NotI (N) or SfiI (S) digestion, with their corresponding size in kilobases, are also shown (37). Figure is from Vary (60).

49.Stevenson, D. M.,, D.Lach,, and P. S.Vary,. A gene required for germination in Bacillus megaterium is plasmid-borne. InE.Balla, and G.Berencie (ed.), Proceedings of the 11th European Meeting on Genetic Transformation. INTERCEPT, in press.

60.Vary, P. S.,1992. Development of genetic engineering in Bacillus megaterium: an example of the versatility and potential of industrially important bacilli, p. 253–311. InR.Doi (ed.). Biology of the Bacilli, Applications in Industry. Butterworth Heinemann, San Diego.