Abstract

An accumulation of major phytoalexin in tomato leaves (Lycopersicum esculeuntum) rishitin were quantified after elicited by abiotic elicitor Silver nitrate (AgNOâ‚ƒ) at different stages of age week three, five, ten, fifteen and twenty using different value of optical density . Rishitin was spotted on Thin Layer Chromatography as a purple fluorescence band at Rfvalue 0.72 under UV light 364nm. No rishitin was detected in control plants at all plant ages. Rishitin were quantified by spectrophotometer at maximum absorbance 500nm and optical density value was taken as a value to compare the presence of rishitin in different ages. The value increased as the plants grew older from three to twenty weeks. The leaves week twenty of tomato showed the highest mean of rishitin value 0.4567 and the lowest was in week three with 0.0067. The data analysis showed that
there was a significant correlation effect between the accumulation of rishitin with plant ages