Description

English: 1= Rab11 and BODIPY-ceramide co-localization. Images of labeled cells (Fig. 4C) were captured in the channels blue, red and green by Z-sectioning in order to obtain 25 optical sectioned images with 0.2 µm of optical section space between each one, covering 5 µm of sample thickness. Images were then deconvolved using the softWorx toolbar. Deconvolved sections were grouped to give a volume perspective of the labeling using the volume viewer tool (softWorx tool bar) and finally saved as a movie showing a rotation of 180 degrees around the Y axis. Imunofluorescence techniques are described in material & methods section. Scale bar = 10 µm.