hygromycin 6ml of 50mg/ml stock per 1000ml of media (6μL of stock per ml of media)

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

*Megan N McClean: Every time you do this protocol you need to check the drug stock concentrations. For example, the last time the G418 stock was made up the activity of the drug wasn't taken into account, which is why it is a 142mg/ml stock instead of a 200mg/ml stock. Double-check the stock solutions and your final desired concentration before making up the media.

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