Background H Pylori infection could be regarded as the commonest infection worldwide, it had been suggested that H pylori infection is more frequent among diabetics. Objective This study had been designed to define any significant association between diabetes and H pylori infection, to evaluate different demographic features of patients with diabetes mellitus who have H pylori infection, assess any relation between H pylori infection and the metabolic control of diabetes mellitus and to estimate the frequency of different oesophagogastroduodenoscopy findings among diabetic population with H pylori infection. Methods This study enrolled 50 patients with diabetes mellitus and another 50 non-diabetic patients (as a control group). The patients attended Gastrointestinal unit at Al-Yarmouk Teaching Hospital Baghdad/Iraq during the period between the 1st of March2004, to the 31st of November 2004. Full history was taken and clinical examination, investigation to assess the glycemic control and esophagogastroduodenoscopy (OGD)had been done to all of them. H pylori status had been detected by positive Rapid Urease test and histopathology and/or ELIZA test for anti-H pylori IgG. Results This study revealed that 26diabetic patients (52%) were H pylori positive, while 14 non-diabetic patients (28%) were H pylori positive which is statistically significant (X2=6.0,P=0.01). patients aging 60 year old or more (24 patients, 48% of the sample) 18 of them had positive H pylori status, 20 patients out of the 26patients with positive H pylori status (76.9%) had poor glycemic control, 84.6% of those with positive H pylori status (22 out of 26 patients) had glycated hemoglobin level of 8% and greater, 61.8% of those with positive H pylori status (16 patients) were diabetic for more than 10 years. 61.5% of those with positive H pylori status had one or more of the chronic diabetic complications, 12 patients had OGD finding consistent with duodenitis, all of them were H pylori positive, OGD examination revealed 20 cases without active disease, 18 of them had negative H pylori status. Conclusions This study indicates that Helicobacter pylori infection is more common in diabetic patients. Frequency of H pylori infection is higher among elderly diabetics, those with long standing, poorly controlled and with diabetic complications.

ABSTRACT:BACKGROUND:Helicobacter pylori has been detected in many populations and associated with inflammation of gastro duodenal mucosa. Colonization of the stomach by Helicobacter pylori occurs in more than half of human population worldwide. It is the principle cause of chronic active gastritis, peptic ulcer and gastric cancer.OBJCTIVE:To detect neutrophiles in homogenates biopsied gastric mucosa semiquantitatively using rapid leukocyte strip test (leukostix).METHODS:A total of 115 patients (74 males, 41 females) referred to The Gastrointestinal Tract Center and Gastroscopy Department of Baghdad Medical City and subjected to gastroscopy were included in this study during the period from November 2004 to May 2005.RESULTS:The sensitivity and specificity of leukostix at the initial examination were 95.8%, 88.23% respectively.CONCLUSION:The leukostix test, using biopsied samples of gastric mucosa was excellent for quantitative determination of neutrophils in patients infected with H pylori

ABSTRACT:BACKGROUND:Helicobacter pylori (HP) cause a continuous gastric inflammation in virtually all infected persons. It induces a vigorous systemic and mucosal humoral and cellular immune response. In spite of these responses, the vast majority of infected hosts are unable to clear the infection and it persists for decades.OBJECTIVE:Determination the humoral immune response to HP infection in dyspeptic patients.PATIENTS AND METHODS:The immunological serological tests were evaluated in 80 dyspeptic patients divided into two groups: (HP +) and (HP-). Levels of specific HP IgG antibodies were determined through a specific immunological non invasive Enzyme Linked Immuno Sorbent Assay (ELISA) test from Biohit PIC, Helsinki, Finland. Immunoglobulin levels and complement were done (IgG, IgA, IgM, C3 and C4) using single radial immune diffusion (BioMaghreb-Tunis).RESULT:About 62.5 % of dyspeptic patients had HP + infection. There was no significant differences between two groups in the levels of (IgG, IgA, IgM, C3 and C4) and most of them were within normal values.CONCLUSION:Humoral immune response had an important role in the control and limits the inflammation of gastric mucosa.

ABSTRACT:BACKGROUND:Helicobacter pylori has been detected in many populations and associated with inflammation ofgastro duodenal mucosa. Colonization of the stomach by Helicobacter pylori occurs in more thanhalf of human population worldwide. It is the principle cause of chronic active gastritis, peptic ulcerand gastric cancer.OBJCTIVE:To detect neutrophiles in homogenates biopsied gastric mucosa semiquantitatively using rapidleukocyte strip test (leukostix).METHODS:A total of 115 patients (74 males, 41 females) referred to The Gastrointestinal Tract Center andGastroscopy Department of Baghdad Medical City and subjected to gastroscopy were included in thisstudy during the period from November 2004 to May 2005.RESULTS:The sensitivity and specificity of leukostix at the initial examination were 95.8%, 88.23%respectively.CONCLUSION:The leukostix test, using biopsied samples of gastric mucosa was excellent for quantitativedetermination of neutrophils in patients infected with H pylori.

This study aimed to assess the accuracy of histopathology, brush cytology, andurease test in the diagnosis of Helicobacter pylori and to evaluate the effect of testduration on the sensitivity and specificity of positive urease test for the detection of H.pylori.Fifty patients [25 patients with gastritis group A and 25 patients with duodenal ulcergroup B] selected from those attending endoscopy unit for dyspeptic symptoms, wereenrolled in the study. Four endoscopic biopsies were taken from each patient. Onebiopsy from each of antrum and body were obtained for urease test (Urease test wasread at 30 min, 1, 4 and 24 hour after biopsy insertion into the reagent), and onebiopsy from each of antrum and body were used for histopathological examination.Antral brush cytology was taken also from each patient. The patients were consideredH. pylori positive when minimum concordances of 2 out of 3 tests (Histopathology,brush cytology, and urease test) were positive.Fourteen patients were positive for H. pylori in group A, in comparison to seventeenpatients in group B. The sensitivities of the histopathological examination, brushcytology, and urease test at 24 hours in group (A) were 58%, 79%, and 93%respectively. Corresponding figures for the specificity were 100%, 91%, and 46%respectively. While in group (B) the sensitivities were 82%, 82%, and 100% and thespecificities were 100%, 100%, and 88% respectively.It is concluded that among the invasive methods, the association of the urease testwith brush cytology constituted the best choice for confirming the diagnosis of H.pylori, due to the high sensitivity of the urease test and high specificity of brushcytology.

Thirty clinical isolates of Helicobacter pylori bacteria obtained from patients attending endoscopy unit of Ibn-Sena teaching hospital in Mosul . These patients were complaining from epigastric pain , dyspepsia , acidity , vomiting , abdominal pain , flatulance , heart burn and melena . The H. pylori isolates were used for Haemagglutination assay (HA) , which involves the recognition of various glycoconjugates on the surface of red blood cells . In this study sheep red blood cells has been used in (HA) assay because the sheep erythrocytes surface resemble that of human epithelial cells .The results proved by (HA) assay, the ability of H. pylori to adherence to specific receptors on the surface of Human Epithelial Cell , which is the first step in the pathogenic process .

Helicobacter pylori (H. pylori) is a gram-negative bacterium that specifically colonizes the gastric epithelium causing many complications. The link between H. pylori infection and diabetes mellitus (DM) remains controversial. This study was conducted to determine the frequency of H. pylori infection among a group of subjects with DM and to compare it with that of an age and sex-matched group of non-diabetic subjects. This case control study was conducted in Al-Sadr Teaching Hospital, Basrah, Southern Iraq from January 2013 to September 2014 on 200 subjects aged ≥ 40 years, 100 diabetic and 100 non-diabetic subjects. All subjects were required to submit fresh stool samples which were tested for evidence of H.pylori infection by stool antigen positivity. Helicobacter pylori infection was detected in 43% of diabetic group and 25% of controls, which was found to be statistically significant ( p value=0.007). In conclusion, the present study suggests that diabetic subjects are at more risk for H. pylori infection in comparison to non - diabetic subjects. This association is found to be higher in those with long duration of DM and those with poor glycemic control.

Helicobacter pylori (H. pylori) is the most important etiologic factor for gastric cancer. It is one of the most common human pathogens, which colonizes in the mucus layer of the gastric epithelium in more than 50% of the population. The study include 78 samples of gastric cancer in addition to 42 blood samples. The results from 78 gastric cancer samples showed that gastric cancer can occur red at any age, but it increases in older ages and the incidence of H. pylori infected positive gastric cancer (HIP-GC) is higher than of H. pylori infected negative gastric cancer (HIN-GC). Male is the predominant in both groups and female affected by H. pylori more than male and the intestinal type was the predominant type.

Helicobacter pylori is a bacterial infection of the stomach, which plays a major role in abdominal symptoms and gastroduodenal pathology. The pregnant women had a significantly higher relative risk of acquiring H.pylori infection during pregnancy as a result of physiological alterations. To investigate the relationship of H.pylori with dyspeptic symptoms in early and late pregnancy, thirty sera samples were obtained from pregnant women and thirty sera samples were obtained from apparently healthy women as control. All studied groups were evaluated anti-Helicobacter pylori IgG antibody by ELISA. Ten of pregnant women samples (33.3%) were seropositive of anti-H. pylori IgG antibody in pregnant women compared with control. This lead to suggest that H. pylori positive may be related to nausea and vomiting in pregnant women.

.ABSTRACTIn Silico Study, was conducted to search and predict essential biotargets whichmight be as drug targets in Helicobacter pylori. Gene sequences (Protein sequences)of the bacterium and its host (Homo sapiens) were retrieved from the Database forEssential Genes (DEG) (http://tubic.tju.edu.cn/deg/).The retrieved sequences were subjected to alignment process using BLASTP2.2.23+(http://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastSearch&PROG_DEF=blastn&BLAST_PROG_DEF=megaBlast&BLAST_SPEC=blast2seq ) withdifferent bioinformatic parameters and different matrices such asPAM70,BLOSUM62 at different levels of E-values "0.001, 0.01, 0.05" to estimatethe essential bacterial genes that non-homologous with the human. Results showedthat 55 genes out of 323 essential bacterial genes have no homology with humanessential genes (118). The products of these genes (55 genes) were subjected toprograms to estimate the cellular location such as PSORTb v.3.0(http://www.psort.org/psortb/index.html) and TMHMM(http://www.cbs.dtu.dk/services/TMHMM/). The two programs showed that 22%of non-homologous genes (12 protein) were associated with cell membrane, 24%(13 protein) had unknown location, and the rest 54% (30 protein) were found inthe cytoplasm. It could be predicted that the membrane proteins might be goodtargets for vaccine development and as well for antibiotics affected the cellmembrane.