Three plots were ® produced from each assay, one representing the Michaelis-Menton equation and the other two representing linearized forms of that equation, specifically a double-reciprocal plot called a Lineweaver-Burk plot and an m max Eadie-Hofstee plot.

• Given a Lineweaver-Burk plot (1/V o vs. 1/[S]) , find/calculate V max and K m from the graph. Learning Objectives, continued •Explain competitive, uncompetitive, and pure noncompetitive inhibition in terms of a diagram of linked reaction equilibria for formation of ES, EI, and (if it can form) EIS.

How close were your estimates to the values from the Lineweaver-Burk plot? 2. How does the concentration of an inhibitor, (I), for competitive inhibition influence: 1) the v o as a function of (S) ; and 2) the Lineweaver-Burk plot?