Viral nucleic acids purified using the QIAamp DSP Virus Kit are ready to use in sensitive downstream applications, such as those based on enzymatic amplification or other modification, including PCR and RT-PCR.

Principle

The QIAamp DSP Virus Kit uses well-established and convenient QIAamp technology for simultaneous purification of viral DNA and RNA. The QIAamp silica-based membrane binds nucleic acids in the lysed sample, while the rest of the lysate is rapidly removed by vacuum pressure. The bound nucleic acids are efficiently washed to remove contaminants and then eluted in a volume of 20 µl or 60 µl.

Procedure

The QIAamp DSP Virus Kit Procedure includes the following steps: lyse, bind, wash (3x), dry spin, and elute. Viral nucleic acids are purified from plasma or serum using a vacuum manifold, such as the QIAvac 24 Plus (see flowchart, "Procedure").

Applications

Viral nucleic acids can be purified from plasma or serum samples. Samples can contain the anticoagulants citrate or EDTA, and can be either fresh, lyophilized, or frozen (provided they were not thawed and refrozen).

The QIAamp DSP Virus Kit provides purification of nucleic acids from a wide range of viruses. The kit is compatible with a wide range of upstream sample collection systems and downstream applications, and therefore can be easily integrated into diagnostic workflows.