Sterol quantification was performed using tetracosane (Sigma Aldrich) as the internal injection standard.

Only three long chain alkanes were detected (tetracontane, tetracosane, heptacosane) which were found in some marine and river sampling stations without revealing a spatial pattern, but showing some consistency with the presence of other terrestrial biomarkers (data not shown).

A mixture of saturated alkanes, such as tricosane, tetracosane and pentacosane, among others, together with rotenone and naphthoquinones 2-acetyl-4H,9H -naphtho[2,3-b]furan-4,9-dione (10) and 2-(1-hydroxyethyl)-4H,9H-naphtho[2,3-b] furano-4,9-dione (11) (Figure 3), were isolated and identified from the hydro-alcoholic extract.

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