The genetic characteristics of white-clawed crayfish populations in Marches Region
(Central Italy) was investigated to plan conservation strategies. In order to clarify their
taxonomic status, 50 specimens from ten populations were collected in six different
hydrographic drainages of the Umbro-Marchigiano Appennine. The genetic relationships
of the Austropotamobius italicus specimens were assessed using DNA sequences of
the mitochondrial DNA 16S rRNA gene.The 3 distinct haplotypes M1, M2, M3 detected
have been compared to those available in GenBank corresponding to four subspecies
described for A. italicus. Our results confirmed the presence of both subspecies A. i.
carsicus (M1 & M2) and A. i. meridionalis (M3) in Marches region. Two mixed populations
with both subspecies have been characterized. These new data of the genetic structure
of population will be used to plan management and recovery programs.

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