PCR of all are part of your sequence should be more than sensitive
to detect your sequence.
Eric
// \\ // \\ // \\ \\ // \\// \\
Eric R. Hugo, Ph.D.// |:,\\': | \\ // :,\\': | \\
You can change your pants, but I // \\ | | \\
can change your genes. \\ | :,\\': |// \\ :,\\
http://w3.one.net/~ehugoSeti at home stats: 1811wu/5.667yrs
budinger at uni-duesseldorf.de (Volker Budinger) wrote in
news:3D5402DA.D4B3A625 at uni-duesseldorf.de:
> Hi,
>> I am characterising some cell lines which were transfected with a
> linearised plasmid. Now i'm searching for a way to find out, how often
> this single sequence was inserted into the genome of those HeLa's.
>> Probably this could be done by southern or FISH but I'am searching for
> other alternative methods instead of these. (Cause I've got problems
> with the sensitivity of the southern assay and fish could not be done in
> our laboratory.)
>> Does anyone know alternative methods?
>> Thanks
> Volker Budinger
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