Eleven is a Python library for performing multi-gene RT-qPCR gene expression normalization. It is a free, open-source implementation of the GeNorm algorithm described by Vandesompele et al. in 2002.

Eleven requires Python 2.7. Earlier versions will not be supported. Python 3.x support is on the roadmap. You will need a Scientific Python stack, including pandas and scipy. If you don’t have these, you can install the free version of the Anaconda environment, which has everything you need.