TY - THES
T1 - Comparative study of using different yeast genera as vehicles for protein delivery to antigen-presenting cells
A1 - Boschi Bazan,Silvia
Y1 - 2011/12/02
N2 - The use of yeasts as vehicle for protein antigens has been demonstrated to be a highly effective vaccination approach. In part, this can be attributed to the intrinsic adjuvant properties of yeast cell wall components. Moreover, the correct processing of recombinantly expressed proteins and the safety status of many yeast genera has encouraged the onset of preclinical and clinical trials using yeast vectors. However, the vast majority of such studies focused the attention on yeasts of the genus Saccharomyces as candidate T cell vaccine. In this work, different yeast genera were evaluated as potential antigen carrier in view of the development of novel yeast-based vaccines. For this purpose, yeasts were initially assessed for their ability to induce maturation and activation of human dendritic cells. Next, the internalization profile of selected yeasts by mammalian phagocytes was analyzed, as well as the involvement of pattern recognition receptors in the uptake process. Subsequently, yeasts engineered to express foreign proteins were assessed for their antigen delivery capacity. In vitro antigen presentation and ex vivo whole blood assays showed that recombinant yeast genera differently activate antigen-specific T cells. Furthermore, antigen localization played a decisive role in T cell activation. The data presented here strongly support the potential of recombinant yeast in the development of novel vaccine strategies in order to induce antigen-specific T cell responses.
KW - Hefeartige Pilze
KW - Immunreaktion
KW - T-Lymphozyt
KW - Antigenpräsentation
CY - Saarbrücken
PB - Saarländische Universitäts- und Landesbibliothek
AD - Postfach 151141, 66041 Saarbrücken
UR - http://scidok.sulb.uni-saarland.de/volltexte/2011/4514
ER -