A research use only product, the ZDC Multiplex RT-PCR Assay is a one-step reverse-transcription, real-time PCR assay for the simultaneous detection and quantification of ZIKV, DENV and CHIKV RNAs from a large range of specimen types. By taking advantage of a multiplex setup that includes the capability to detect all the three viral targets and up to two different controls in a single tube, the assay is a sensitive tool that maintains linearity and efficiency, while also providing a streamlined workflow.