Can I target introns, UTRs, promoters, or enhancers?

The current version of the CRISPR Design Tool identifies guide RNAs that can knockout a gene by targeting the protein coding sequence. If you do not see recommended guide RNAs in introns, UTRs, promoters, or enhancers, it is because these regions have not been shown to be optimal for generating a gene knockout.

We are still investigating UTRs, area-based targeting and other scoring methods to improve the quality of the recommendations to create a gene knockout.