Hello again, again...
I think I figured out why I can't cut the inverted repeats in my plasmid
with restriction endonucleases: The reference is D.M. Lilley and L.R.
Hallam, J Biomol Struct Dyn 1: 169-82 (1983). The abstract says that some
inverted repeats in negatively supercoiled plasmids may form a cruciform
structure that is resistant to cleavage by restriction enzymes, but is
hypersensitive to single-strand-specific nucleases like S1.
Well, ya learn something new every day. Anyway, I'll dig around a bit
before I beg for help next time. Or not...
Ciao,
Ed Beaty
Ed_Beaty at qms1.life.uiuc.edu
==========
"We will find you acting on your best behaviour,
Turn your back on Mother Nature
Everybody wants to rule the world..."
--Tears for Fears