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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is
committed to providing our customers around the world with the highest quality products.
In addition to direct selling in more than 20 countries in North America, Europe and
Asia/Pacific, Miltenyi Biotec also provides support for our customers through an
extensive distributor network covering dozens of additional countries.

As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is
committed to providing our customers around the world with the highest quality products.
In addition to direct selling in more than 20 countries in North America, Europe and
Asia/Pacific, Miltenyi Biotec also provides support for our customers through an
extensive distributor network covering dozens of additional countries.

Background information

Human as well as rhesus monkey dendritic cells represent a heterogenous population composed of several subsets. Myeloid and plasmacytoid DCs, differentiated by their expression of CD11c and CD123, respectively, were further characterized by means of new markers against human blood dendritic cell antigens (BDCA). Two human myeloid dendritic cell populations have been described: CD11c

high

CD123

low

CD1c (BDCA1)

+

DCs and CD11c

dim

CD123

–

CD141 (BDCA-3)

+

DCs. Plasmacytoid CD11c

–

CD123

+

dendritic cells are characterized by the expression of CD303 (BDCA-2) or CD304 (BDCA-4).

1

In rhesus monkey, CD1c is expressed on myeloid CD11c

+

CD123

–

dendritic cells

2

as well as on a subset of B cells. The CD1c and CD20 antibodies have been tested to cross-react with antigens of the cynomolgus monkey (

Macaca fascicularis

).

Applications

The CD1c (BDCA-1)

+

Dendritic Cell Isolation Kit can be used for the separation of CD1c

+

dendritic cells from rhesus monkey (

Macaca mulatta

) PBMCs.

Columns

For the first magnetic separation (depletion): LD or autoMACS

®

Columns. For the second magnetic separation (positive selection): MS, LS, or autoMACS Columns.

Figure 1

Isolation of CD1c (BDCA-1

)+

dendritic cells from rhesus monkey PBMCs using the CD1c (BDCA-1)

+

Dendritic Cell Isolation Kit. Cells were incubated with CD1c (BDCA-1)-PE and subsequently magnetically labeled with CD20 MicroBeads. B cells were depleted using an LD Column. The B cell depleted fraction was magnetically labeled with Anti-PE MicroBeads and separated over an MS Column. Cell samples were stained with CD20-FITC and CD14-APC. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.