Abstract

Dendritic cells (DCs) are potent antigen‐presenting cells (APC) that are important in the initiation and control of cellular
immune responses. Commonly used in T cell–stimulation experiments, DCs are typically “matured” in vitro with microbial products
or proinflammatory cytokines, and then loaded with antigens from any number of sources, including peptides, whole proteins,
cell lysates, RNA, microbes, or killed tumor cells. This unit presents a simple and commonly used method for the generation
of mature human dendritic cells—differentiating them from peripheral blood monocytes.

NOTE: All solutions and equipment coming into contact with live cells must be sterile, and aseptic technique should be used accordingly.NOTE: All culture incubations should be performed in a humidified 37°C, 5% CO2 incubator unless otherwise specified.