The organization has called for the New York City Conflicts of Interest Board to look into the issue, saying the nature and timing of the contribution is troubling.

StudentsFirstNY posted a letter on its website Wednesday that Jenny Sedlis, the organization’s executive director sent to the Conflicts of Interest Board.

“I urge the board to immediately look into these questions,” Sedlis said in the letter. “New Yorkers deserve to know whether it is a conflict of interest for Mayor de Blasio to take large Super PAC contributions from a union with whom he is negotiating a new contract.”

Sedlis also criticized the educational value of de Blasio’s agreement with UFT, questioning why a pay increase came with a two-and-a-half hour decrease in weekly instructional hours.

“The city entered the talks with a strong bargaining position yet it wound up paying significantly more while giving teachers the right to work less,” Sedlis wrote.

De Blasio’s office had not returned requests for comment on the issue. However, a spokesman toldCrain’s the donation had “zero” impact on the mayor’s agreement with UFT.

Whether the board would investigate following Sedlis’ letter was not immediately clear.

If it does, the conflict would likely be investigated as a violation of gift rules, which exist to prevent public figures from using their position for personal gain, offering preferential treatment, or losing their independence in making official decisions.

Even appearing to have broken any such rules can be subject to an investigation.

Wayne Hawley, deputy executive director of general counsel at the board told the WashingtonFree Beacon the board was not permitted to disclose information about investigations, nor could it confirm whether it had received Sedlis’ letter.

However, findings of violation are made available to the public when they occur, Hawley said.

CONY was founded as UPKNYC in November after de Blasio took office and has since raised $1.8 million in contributions.

A reporter at a press conference on Thursday asked de Blasio about the nonprofit’s donors.

“I don’t think they expect anything and I would never feel comfortable if they did,” de Blasio said about donors who might expect something in return for contributions.