Figure 2: mRNA expression of the hepatic genes that are involved in fatty acid oxidation or adipogenesis. The left panel shows an RT-PCR analysis of (a) A1R, A2AR, A2BR, and A3R (b) PPARα, CPT-1, MTP, LCAD, and MCAD and (c) PPARγ, RXR, C/EBPα, LPL, aP2, and FAS expression. The images show the representative agarose gel electrophoresis of the PCR products. The right panel shows the relative expressions of (a) A1R, A2AR, A2BR, and A3R (b) PPARα, CPT-1, MTP, LCAD, and MCAD and (c) PPARγ, RXR, C/EBPα, LPL, aP2, and FAS levels. The data were normalized to the GAPDH levels, and all the expression levels that are displayed are relative to the ND. The results that are shown represent the means ± SEM of the three independent experiments in which RNA samples from eight mice were used. a,b,cMeans not sharing a common letter are significantly different (𝑃<0.05).