Cosmid isolation

I have used the standard alkaline lysis protocol to isolated cosmids
with no problems. The only change I make is to first resuspend the
bacteria in 0.3 M sucrose/25 mM EDTA/25 mM Tris-HCl pH 8.0 + 2 mg/ml
lysozyme and incubate for 5-10 min before lysis. Also, don't vortex the
tubes, just shake back and forth to mix.
Steve