应用

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用

Ab评论

说明

Flow Cyt

Use at an assay dependent concentration. ab37373-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF

Use a concentration of 1 - 5 µg/ml.

WB

Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).

靶标

功能

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

组织特异性

Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.

ab27985 staining Oct4 in human embryonic carcinoma cells by Flow Cytometery. Cells were fixed in paraformaldehyde and permeabilized in saponin buffer. The primary antibody was diluted 1:500 (PBS + 10% BSA) and incubated with sample for 1 hour at 4°C. A FITC conjugated Donkey polyclonal to goat IgG, diluted 1:200 was used as secondary. The red histogram is secondary only control whilst green and blue repersents Oct4 expression in untreated and partially differentiated with 10nM Trans-Retinoic acid for 14 days.

Based on the information we have for this antibody, it may be that it is not suitable for IHC-Fr on sections. However based on the information you have provided, you could try to increase the primary antibody c...

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The closest match we have to ab28445 is ab96930. This is an anti-goat IgG, raised in rabbit, conjugated to DyLight® 650, which has an excitation maximum at 654nm and an emission maximum at 673nm. The Alexa Fluor 680 fluo...