IRE1 alpha Antibody (PA5-20191)

Performance guaranteed

Purchase with confidence, knowing that we stand behind
the performance of our antibodies with the InvitrogenTM
antibody performance guarantee. If an InvitrogenTM antibody
doesn't perform in your experiment as described on our website
or datasheet, we'll replace the product at no cost to you, or
if you prefer, we'll provide you with a credit for a future
purchase.*
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Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Tested Applications

Dilution *

Western Blot (WB)

1-2 ug/ml

* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the
product for use in their own experiment using appropriate negative and positive controls.

Product Specific Information

A suggested positive control is Raji cell lysate.

PA5-20191 can be used with blocking peptide PEP-0321.

Background/Target Information

Accumulation of malfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) and the upregulation of the ER molecular chaperones GRP78 and GRP 94. These proteins are normally bound to ER transmembrane proteins such as IRE1p and ATF6 but ER stress causes their dissociation. This allows IRE1p, a serine-threonine protein kinase to transduce the unfolded protein signal from the ER to the nucleus. IRE1p also has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA converting it to a potent UPR transcriptional activation. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.