Summary

Secondary Antibodies

Negative Isotype Controls

Useful Reagents

Positive Controls

Histology Controls

References

Reviews

-

Mouse anti Human TRF1 antibody recognizes human telomeric repeat-binding factor 1, also known as TTAGGG repeat binding factor 1 (TRF1) or NIMA-interacting protein 2. TRF1 is a ubiquitously expressed 439 amino acid DNA binding protein and negative regulator of telomere length containing a single Myb domain. Along with tankyrase-1, TIN2, and PINX1, TRF1 binds the double-stranded TTAGGG repeat of telomeres and regulates telomere length. Protection of telomeres-1 (POT1) is a single-stranded telomeric DNA-binding protein which controls the elongation of telomeres by telomerase. TRF1 mediates the loading of POT1 onto single stranded DNA, conveying information about telomere length to the telomere terminus, where telomerase is regulated. If the telomere is long enough, telomerase is inhibited.

Additionally, in conjunction with POT1 and TTAGGG repeat binding factor 2 (TRF2), TRF1 recruits factors to the telomere, inducing the formation of the protective t-loop. TRF1 along with TRF2, POT1, TIN2, TPP1 and Rap1 form the shelterin complex which distinguishes telomeres from sites of DNA damage (Wellinger 2009). TRF1 is thought to play a role in leukaemia (Shi et al. 2006).

Mouse anti Human TRF1 antibody recognizes human TRF1 as a single band of ~60 kDa in multiple human cell line lysates by western blotting under reducing conditions.

Target Species

Human

Western Blotting

Anti TRF1 antibody detects a band of approximately 60 kDa in K562 cell lysates.

Anti TRF1 antibody detects a band of approximately 60 kDa in K562 cell lysates.

Anti TRF1 antibody detects a band of approximately 60 kDa in K562 cell lysates.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name

Yes

No

Min Dilution

Max Dilution

Western Blotting

1/1000

Application Name

Yes

No

Min Dilution

Max Dilution

Western Blotting

1/1000

Application Name

Yes

No

Min Dilution

Max Dilution

Western Blotting

1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.