Selection and optimization of new regulatory elements for the construction...

Selection and optimization of new regulatory elements for the construction of cell-based biosensors for detection of antimicrobial agents

Period

01 / 2009 - 12 / 2013

Status

Current

Research number

OND1337226

Abstract

Description:Cell-based biosensors provide a fast and efficient strategy for detection of contaminants in food and other matrices. Development of these sensors is limited by the availability of regulatory elements that respond to the presence of a specific agent. This project involves the development of an approach for the selection and identification of new regulatory elements with a suitable specificity for application in cell-based biosensors.

Research objectives:Selection of new elements will be achieved using phage-display. As a first proof of principle, a well-characterized regulator will be cloned in the phage-display system. A binding assay with the associated target molecule will show the feasibility of the strategy. Subsequently, a (bacterial) genomic phage-library will be constructed, which will be subjected to affinity selection (panning) with an appropriate target.

Results and products:The project will deliver a method for the selection of new regulatory elements for application in cel-biosensors.