If you are referring to a liquid spawn media, using a spore or tissue
source, Paul Stamet's "Growing Gourmet and Medicinal Mushrooms" has an
excellent chapter on it, along with the receipe and recommended agitation
methods.
If you don't have the book available, e-mail me and I can give you a
synopsis of the procedure. But the text is really indespensible.
Joe
On 19 Sep 1995, C.C.Chen wrote:
> Date: 19 SEP 1995 06:48:03 -0700
> From: C.C.Chen <r3603207 at CC.NTU.EDU.TW>
> Newgroups: bionet.mycology
> Subject: culture condition in fermemtation process
>> How design a method to optimize the cultural conditions ?
> for example C source , N source , trace element , pH ,Temp , agitation
> ...............
>>>