Dear Hubert,
Yeast genomic clones and expression plasmids are available
commercially, but I'm not aware of any source for plasmids that could
be used to make knockout strains. Your best bet would probably be to
look at the literature for GPD1 and GPD2, which is easily done by
clicking the 'Literature Guide' link on the SGD locus pages for these
genes (http://db.yeastgenome.org/cgi-bin/SGD/locus.pl?locus=gpd1 and
http://db.yeastgenome.org/cgi-bin/SGD/locus.pl?locus=gpd2), to find the
names of researchers who have constructed deletions. They might be
able to provide you with the necessary clones or reagents.
If the newsgroup emails are considered spam by your server, that's
something you'll have to take up with your network administrator or
ISP. I receive the newsgroup posts myself as email, coming through a
commercial ISP, so I know the system works. Maybe you could ask your
ISP to allow incoming mail from the yeast group address.
Good luck, and let me know if you have further questions.
Best regards,
Maria
Maria C. Costanzo, Ph.D.
Senior Scientific Curator, Saccharomyces Genome Database
Department of Genetics
Stanford University School of Medicine
Stanford, CA 94305-5120
Phone: 650-725-8956
Fax: 650-723-7016
http://www.yeastgenome.org/maria at genome.stanford.edu
On Tuesday, June 17, 2003, at 11:41 PM, Hubert Piatkowski wrote:
> Hi folks..
>> I'm very new to this as I have not done any mol bio in 8 yrs or so
> (back
> in school).
>> I'm interested at doing some gene deletions with my strain of yeast
> that I
> use for fermenting corn to alcohol. Since I'm not interested in high
> glycerol content I need to play around with GPD2 and GPD1 genes. How
> would I
> go about removing these. I could perform a gene knockout but I would
> have to
> make a plasmid first to replace these genes, are these available
> commercially
> or do I have to manufacture one??
>> Also, how do I get on the mailing list?? I tried subscribing, and the
> server bounces my mail as SPAM...
>> Any help would be greatly appreciated..
>> Thanks
>> Hubert Piatkowski
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