Answers

We supply customers throughout North America. At this time, we do not supply fresh human hepatocytes to researchers in countries outside these regions.

2. Where do you get your liver tissue?

Life Technologies sources liver tissue from a network of hospitals that perform surgical resections on living donors, as well as from deceased donors whose organs were rejected for transplant. Sourcing for North American customers occurs in North America.

3. What are the quality specifications on your fresh human hepatocytes?

Typically, we have access to one or more human liver tissues per week, ensuring a consistent supply.

5. Are there guidelines for the procurement of human liver tissue?

Yes, we comply with country-specific legal and ethical standards for procurement of human liver tissue, including the global ICH Guidelines, and the US’s HIPAA, Uniform Anatomical Gift Act, National Organ Transplant Act, and hospital internal review board (IRB) approval processes. 6. What is a service level agreement (SLA) and do I need one?

If you are working in a laboratory in the European Union ONLY, you will need to have a service level agreement (SLA) on file with us. An SLA is designed to comply with the UK’s Human Tissue Authority (HTA) regulations and outlines the scope of use for human biologicals. As an end-user, you must acknowledge that the use of our product has had ethical review and approval. 7. What is a Rapid Alert?Rapid Alerts notify you of the impending availability of fresh primary human hepatocytes. Upon receiving a Rapid Alert email, you decide if you need hepatocytes for an upcoming study and can contact us immediately to place an order. Each Rapid Alert contains:

a summary of the donor’s demographics, such as gender, age, race, BMI, and history of alcohol, drugs, or medications

the plate and suspension formats available for order

the date when the hepatocytes will be shipped to you

a phone number and email to place your order or request additional information

8. Can I place an order for fresh human hepatocytes if I am not registered for Rapid Alerts?

No, we must be aware of your interest in freshly isolated human hepatocytes ahead of time. We take orders based on customer response to our Rapid Alert.

Once you receive a Rapid Alert and determine you want to place an order, reply to the message or call the number listed. Orders are placed prior to the isolation. Prior to ordering, you must have:

an approved account with Life Technologies

a valid purchase order

In North America, purchase orders are made out to Life Technologies. Credit cards are accepted for North American customers. 11. Can I call Life Technologies Customer Services for fresh hepatocytes?

No, the customer service team that helps you order other Life Technologies products does not take orders for fresh hepatocytes. You must respond to the email or phone number that is listed on the Rapid Alert.

12. If I respond to a Rapid Alert, does that guarantee I will be able to receive fresh human hepatocytes?

No, there is no guarantee you will receive hepatocytes. You will not receive fresh human hepatocytes if:

isolation results do not meet our release criteria

customer demand exceeds supply

13. How quickly do I need to respond to a Rapid Alert?

Almost all of our orders are taken within two hours of a Rapid Alert. To facilitate your order, we recommend a pre-approved blanket purchase order.

14. Can you notify me of fresh hepatocyte availability through my Twitter account?

We are not sending broad-based Twitter alerts at this time. Please discuss any special notification requirements with one of our Hepatic Product Specialists.

15. How will the fresh hepatocytes be shipped to me?

Our hepatocytes are shipped FOB Origin from Durham, NC (North America orders). Fresh suspension cells are sent in cold preservation solution via courier/commercial shippers and delivered as soon as possible. Plated hepatocytes are shipped via courier/commercial shippers one to two days post-plating. This is to ensure good cell health and stabilized CYP450 levels.Courier shipping costs are $300 and commercial shipping costs range between $35 and $100, depending on your region. A standard $75 handling fee is added to each order.

16. How should I handle the fresh hepatocytes once I receive them?

Upon receipt, fresh hepatocytes in suspension should be centrifuged at 4ºC immediately, following our instructions. Plated hepatocytes should be quickly removed from the box and the shipping medium replaced with fresh culture medium. The preservation medium used during shipment is meant to be kept cold; if the cells warm past 8ºC, the shipping medium begins to have a toxic effect on the hepatocytes. We recommend that you let your plated cells acclimate overnight before beginning your experiment.

17. Do you sell medium to use with my fresh hepatocytes?

Yes, you can order Williams Medium E and supplements for plating and maintaining primary hepatocytes in short-term culture from Life Technologies. Medium can also be ordered and shipped along with your hepatocytes.

18. Why purchase my fresh hepatocytes with overlay?

A more three-dimensional, liver-like framework can be modeled in vitro using a Geltrex overlay, which can extend the life of a healthy hepatocyte culture for more chronic applications such as induction or hepatobiliary transport. A Geltrex overlay is typically recommended for time-points more than 48 hours after isolation. A Geltrex overlay would not be recommended for use before transfecting hepatocytes, or for use in protein secretion studies.

19. Who can I call if I have technical questions?

Invitrogen has a dedicated team to answer questions regarding hepatocyte cell culture. You can send a question by email or contact us by phone: US toll-free: +1 866 952 3559 US toll: +1 919 237 4679

Thermo Fisher Scientific

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