Dear fellows,
at first I want to thank all those who made comments and suggestions to
my inquiry. Many people are in a similar situation with rubisco. Here is
a short summary of the suggestions I received, maby one or the other
will be helpful:
removal of rubisco by:
1) a PEI-agarose column
2) a short heat treatment of the extract (60 degrees for 2 minutes,
Ravanel et al., 1995
Arch Biochem Biophys 316:572)
3) 50% more electrophoresis time than needed to run the BPB to the
bottom of the gel
4) extract from dark grown seedlings. Alternatively, place light grown
plants into the dark for a day or
two.
5) native-page gel
Thanks again and good luck
Ralf
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Ralf R. Weigel
Albrecht von Haller Institute of Plant Sciences
General and Developmental Plant Physiology
Untere Karspuele 2
37073 Goettingen
Germany
Tel.: 0551/3919664
Fax: 0551/397820
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