In addition, Bernier et al, in collaboration with our laboratory, has demonstrated that overexpression of apoE in adipocytes reduces differentiation as well as the accumulation of cholesterol and triglycerides in these cells [7].

Next, oligonucleotides containing the sequence for the ApoE ligand (LRKLRKRLLRDWLKAFYDKVAEKLKEAF) (synthesized by Integrated DNA Technologies; Coralville, Iowa, USA), flanked by AscI and PacI sites, were annealed, cut with AscI and PacI, and inserted into the corresponding sites of pAAVRCKI to make pAAV2apoE.

LexA-NRC (NRC containing AD1 and AD2 activation domains) when expressed in yeast was not found to be constitutively active, however, 100 fold activation was detected when RXR without any heterologous activation domain was co-expressed in the presence of its ligand.

In animal models and in humans it has been observed that diets rich in these substances or containing a supplement of them (omega-3 fatty acids, docosahexaenoic acid) reduce AD risk, especially in individuals who do notcarry the Apo E ?

There are virtually no loci, with the possibleexception of APOE (Burwick et al., 2006), where published studies have been adequately powered to confidently exclude the possibility of a meaningful effect.

Recent data suggest a genetic predisposition for cognitive decline after cardiac surgery in patients possessing the apolipoprotein E epsilon-4 allele, known to be associated with late-onset and sporadic forms of Alzheimer's disease.