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i have nine pf jars inoculated with about 8 cc's apiece of liquid myc, they have been sitting in a TiT for 2 days now at around 82-84F and there are no signs of any growth . is this normal or should something have shown up already?

8 cc's a piece? If you used that much spore solution in each jar, you surely threw off the water content of the jars, if they are standard 1/2 pint jars. You might have to toss them and start over. 1cc per jar is more than enough. Still, 2 days, even with myc culture, is a little early to be worrying. Depending on your parameters, it might take a few more days to see growth.

--------------------Everything that we see is a shadow cast by that which we do not see.

wait a couple more days. i have waited as long as 5 days before i have seen any growth. 8cc's a jar is way to much. lifenergy is right about the water content. also it is a waste. if you used 8cc's a jar on nine jars that is 72cc's that you used. with that much you could of had 48 jars on the low end and 72 on the high end. oh well next time you know, good luck.

if you want to do big injections , you need to reduce the water content when making your jars. This loses its effectiveness past 5cc. So like If you normally make your jars with 60cc of water, make it with 56cc, once its pc'ed and ready to go inject divide 5cc into the four holes(5cc total). Make sure you flip your jars every coupld days to help distribute the liquid. more myc, more growth sooner, but past 5cc , not much point.

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