The game is extremely simple. The player is offered a pair of X-Ray glasses by a Gypsy Man. If the player refuses, they are killed. If they accept, they get the chance to explore their surrounding environment. There are four scenes to look at, each with multiple people. By hovering the mouse over different people, their bodies beneath their clothing are revealed, including genitalia. However, if the player looks at the Gypsy man's penis with the glasses, he will kill the player in retaliation, however if the player holds the mouse button while hovering on the gypsy, his genitalia can be revealed as well.

Considering how the game is a decade old, Cooties Bar X-Ray Glasses has an impressive presentation. Humans are portrayed with realistic figures and proportions, and a fair amount of action occurs in the background. The game features a music loop, sound effects, and even genuine voice acting.

Despite the game's simplicity, it is one of the most viewed submissions in Newgrounds history. As of January 2012, it has over 29.2 million views, making it the fifth most viewed submission on Newgrounds. The game also won a Daily Feature, and has a respectable review average of 8.1/10 despite its age. The game is also featured in the Flash Portal History, Adult Games, and Gadgets Collections.

Play it hereWARNING: This link contains material which is inappropriate for viewers under the age of 18. If you are under 18 and/or are offended by adult content, do not follow this link.