Biophysicshttp://www.scientistsolutions.com/c308-biophysics.html
Interdisciplinary field applying techniques from the physical sciences to understanding biological structure and function at the molecular level.
Life Science Discussionen-ussci7feed@sci7.nojunkorherepleasespam.comhourly12005-05-05T12:00+00:00Postdoctoral Fellow in Biomedical Physicshttp://www.scientistsolutions.com/t35641-postdoctoral+fellow+in+biomedical+physics.html
The Biomedical Physics research group (http://medicalphysics.ok.ubc.ca/jirasek.html and http://web.uvic.ca/~agbrolo/) invites applications for a Postdoctoral Fellow with expertise in the areas of biomedical Raman spectroscopy. The preferred candidate will have experience in the following areas:

Basic Function: The succ...]]>Tue, 22 Jul 2014 12:51:29 GMTPhD student in Biophysics and Nanobiotechnology: Linnaeus Universityhttp://www.scientistsolutions.com/t29980-phd+student+in+biophysics+and+nanobiotechnology_+linnaeus+university.html
Formal research area: Biomedical SciencesResearch in biomedical sciences and biotechnology at Linnaeus University is performed within the Faculty of Health and Life Sciences where strong research areas include molecular biophysics and biotechnology, the focal points of the current project.Applicants should have a Master deg...]]>Fri, 09 May 2014 10:09:04 GMTTwo Electrode Oocyte Voltage Clamphttp://www.scientistsolutions.com/t29905-two+electrode+oocyte+voltage+clamp.html
now, and I have not been able to get expression of our protein (M2 channel). What would be the problems that inhibitexpression of our protein in oocytes? Our protein has been purified, shows up very brightly on a gel, it's been sequenced,etc...the mRNA is just fine, but I inject at least 25 oocytes every week with the wild type mRNA and I never get any expre...]]>Fri, 02 May 2014 17:22:10 GMTProblem with a Dagan PC-One patch-clamp amplifierhttp://www.scientistsolutions.com/t29414-problem+with+a+dagan+pc_one+patch_clamp+amplifier.html
The first one is not obeying the command of the junction, then there's no way to make the eletrodes compensation, I think it's a problem with the headstage. I don't know, what can I do?

In the second one, the valve of voltage is unregulated, the display demonstrates a value and the value of the voltage applied is another. And in some moments, the voltage clamp doesn't work greatly, it is no clamping.&n...]]>Fri, 07 Mar 2014 13:25:20 GMTHum Bug on patch clamp righttp://www.scientistsolutions.com/t29113-hum+bug+on+patch+clamp+rig.html
I would like to set up a Hum Bug noise canceler on my patch clamp rig. I am not clear from the instructions how to do this. Do I plug it into my Digidata? Both the input and output?Any help would be appreciated.Jenna.]]>Wed, 05 Feb 2014 15:36:13 GMTNo current in HEK cellshttp://www.scientistsolutions.com/t29083-no+current+in+hek+cells.html
I use 10 mM Ba2+ and it used to work perfect before. PAssage nr is 30. Any idea? Can it be a hardware problem?CheersPeter]]>Tue, 28 Jan 2014 05:08:59 GMTMacro Particle acceleratorhttp://www.scientistsolutions.com/t27734-macro+particle+accelerator.html
Fri, 02 Aug 2013 20:34:13 GMTWhat will be the right courses for me in my collegehttp://www.scientistsolutions.com/t27477-what+will+be+the+right+courses+for+me+in+my+college.html
Mon, 08 Jul 2013 04:02:27 GMTWhole Cells in Patch Clamphttp://www.scientistsolutions.com/t26998-whole+cells+in+patch+clamp.html
We use HEK cells transiently transfected with a K+ channel. It seems that when cells are &ldquo;driving&rdquo; a current the seal is always broken before to achieve good a whole-cell. But it works well with HEK cells non-transfected, HEK cells transient...]]>Tue, 28 May 2013 23:48:33 GMTMyotonic dystrophy: misreg'd splicing & gating of Ca(V)1.1 Ca++ channelhttp://www.scientistsolutions.com/t26278-myotonic+dystrophy_+misreg_d+splicing+_amp%3b+gating+of+cav1_1+ca%2b%2b+channel.html
]]>Mon, 18 Mar 2013 09:42:47 GMTUnable to output file from fluorescence spectrometer (.td)http://www.scientistsolutions.com/t26172-unable+to+output+file+from+fluorescence+spectrometer+_td.html
I've done a experiment on a fluorescence spectrometer (not the one I usally use) , and I recieved the output in a .td formate. I asked in the lab and a post doc told me the he used spekwin32, to read the data. When I came home I downloaded this program but I'm unable to open the files of interest. Tried every possible way to open them.I would really love to get down on the data already to night, so if anyone got any alternative programs or any hints on how to use spekwin32...]]>Thu, 07 Mar 2013 09:18:48 GMTCardiomyocyte PatchClamphttp://www.scientistsolutions.com/t25488-cardiomyocyte+patchclamp.html
Is anyone here doing patch clamp on cardiomyocytes?I have a problem isolating viable cells. My cells are too fragmented and I barely get single cells.I use the enzymes collagenase and Protease and my buffer has MgCl2, KCl, NaCl, HEPES, glucose, CaCl2Please let me which protocols you useThank you]]>Mon, 14 Jan 2013 19:15:55 GMTCurrent, voltage and bridge balance problemshttp://www.scientistsolutions.com/t24565-current%2c+voltage+and+bridge+balance+problems.html
So I'm setting up my first rig for intracellular electrophysiology.I'm using an Axoclamp 2B amplifier and pClamp 9 software, and I have calibrated the software to the Axoclamp. I haven't tried to impale anything yet. This problem starts when I simply place my electrode into the extracellular solution.My problem is when I inject current down my electrode, the change in voltage occurs in the wrong direction. For example, if I inject a hyperpolarising step to remove series ...]]>Wed, 24 Oct 2012 18:00:49 GMTNext Generatiion science developedhttp://www.scientistsolutions.com/t23734-next+generatiion+science+developed.html
FIT or Certainty tool technology developed which is next Generation science.World scientist are welcome to see and test FIT technology.vk sonakia Unified Field ScientistIndiavksonakia@yahoo.co.in]]>Sun, 05 Aug 2012 00:26:50 GMTCharacterization of Zebrafish Retinal Connexin Hemichannelshttp://www.scientistsolutions.com/t23166-characterization+of+zebrafish+retinal+connexin+hemichannels.html
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Sat, 28 Apr 2012 02:42:40 GMTSetting up an electrophysiology righttp://www.scientistsolutions.com/t22742-setting+up+an+electrophysiology+rig.html
I am in the process of setting up an electrophysiology rig for the first time. Any advice on the best way to go about it. I have all the components, microscope, manipulator airtable etc.Cheers,Kwai]]>Mon, 09 Apr 2012 11:11:32 GMT