Nite Owl 5N connects the city areas of Marina Centre, City Hall, River Valley and Orchard with Farrer and the estates of Clementi, Jurong East and Jurong West. This service only operates on Fridays, Saturdays, eves of Public Holidays, with four trips on regular operating days.

The route has an express sector along Bukit Timah Rd (after Little India MRT) to Farrer Road,. It also does not call at selected bus stops along Holland Rd, Ulu Pandan Rd, Commonwealth Ave West and Boon lay Way.

During festive periods such as Christmas Eve or New Years’ Eve, operating hours are extended to 4am to cater for late-night dwellers.