Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla

抄録

Prokaryotic diversity in Aran-Bidgol salt lake, a thalasohaline lake in Iran, was studied by fluorescence in situ hybridization (FISH), cultivation techniques, denaturing gradient gel electrophoresis (DGGE) of PCR-amplified fragments of 16S rRNA genes and 16S rRNA gene clone library analysis. Viable counts obtained (2.5—4 × 10<sup>6</sup> cells mL<sup>-1</sup>) were similar to total cell abundance in the lake determined by DAPI direct count (3—4×10<sup>7</sup> cells mL<sup>-1</sup>). The proportion of <i>Bacteria</i> to <i>Archaea</i> in the community detectable by FISH was unexpectedly high and ranged between 1:3 and 1:2. We analyzed 101 archaeal isolates and found that most belonged to the genera <i>Halorubrum</i> (55%) and <i>Haloarcula</i> (18%). Eleven bacterial isolates obtained in pure culture were affiliated with the genera <i>Salinibacter</i> (18.7%), <i>Salicola</i> (18.7%) and <i>Rhodovibrio</i> (35.3%). Analysis of inserts of 100 clones from the eight 16S rRNA clone libraries constructed revealed 37 OTUs. The majority (63%) of these sequences were not related to any previously identified taxa. Within this sampling effort we most frequently retrieved phylotypes related to <i>Halorhabdus</i> (16% of archaeal sequences obtained) and <i>Salinibacter</i> (36% of bacterial sequences obtained). Other prokaryotic groups that were abundant included representatives of <i>Haloquadratum</i>, the anaerobic genera <i>Halanaerobium</i> and <i>Halocella</i>, purple sulfur bacteria of the genus <i>Halorhodospira</i> and <i>Cyanobacteria</i>.<br>

収録刊行物

Microbes and environments

Microbes and environments 27(1), 87-93, 2012-03-01

Japanese Society of Microbial Ecology · The Japanese Society of Soil Microbiology