Universal Mycoplasma Detection Kit (ATCC® 30-1012K™)

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The Universal Mycoplasma Detection Kit offers a quick and sensitive PCR-based test to detect mycoplasma contaminants in cell culture. All components required for the PCR reaction are provided and have been optimized for amplification. High specificity is obtained through the utilization of a proprietary mix of buffers, dNTPs and thermostable polymerase, combined with universal primers that are specific to the 16S rRNA coding region in the mycoplasma genome. DNA originating from other sources, such as tissue samples or other bacteria, are not amplified. A touchdown PCR regimen increases sensitivity of the assay, along with enhancing specificity.

The kit detects over 60 species of Mycoplasma, Acholeplasma, Spiroplasma and Ureaplasma including the eight species most likely to afflict cell cultures: M. arginini, M. fermentans, M. hominis, M.hyorhinis, M. orale, M. pirum, M. salivarium, and A. laidlawii. Samples that are positive for mycoplasma are easily recognized by a distinct PCR product ranging in size from 434 to 468 bp on an agarose gel.

Component

Volume

Composition

Storage

Lysis Buffer

2 mL

Lytic agent + digestive enzymes

-20°C

Universal PCM Mix

0.8 mL

Proprietary mix of buffers, dNTPs, thermostable polymerase

-20°C

Universal PCR Primers

0.1 mL

Proprietary mix of universal forward and reverse primers

-20°C

Sample Lysis Tubes

40 each

2 mL snap cap tubes, tight seal

-20°C

Positive Control

50 µL

1 pg/µL pUC19::M. arginini target in TE

-20°C

Type Strain

no

Biosafety Level

1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.