Title : THE GLOMERULAR FILTRATION OF HEMOGLOBIN: A PROPOSED MECHANISM.

Descriptive Note : Final rept.,

Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s) : Bunn,Howard F. ; Bull,Robert W. ; Jandl,James H.

Report Date : 18 AUG 1967

Pagination or Media Count : 12

Abstract : The relative ease with which hemoglobin is filtered by the kidney suggests that the molecule may enter the glomerulus primarily as a half molecule. Glomerular filtration of hemoglobin was assayed by the accumulation of kidney radioactivity following the injection of iron-59 labeled hemoglobin to haptoglobin depleted animals. In rats the renal uptake of BME hemoglobin (a form shown to have a reduced degree of symmetrical dissociation into dimers) was only one-fourth that of labeled oxyhemoglobin. Shortly after the injection of deoxyhemoglobin into the renal arteries of rabbits, the kidney activity was about two-thirds that of animals receiving oxyhemoglobin. Renal uptake was enchanced by progressive dilution of the injected oxyhemoglobin. These results support the hypothesis that hemoglobin is filtered through the kidney primarily as an alpha-beta dimer. (Author)