Based on data from several studies, JCVs were
calculated for for Marseillevirus, Lausannevirus, Tunis
virus, Cannes 8 virus,
20 and Insectomime virus.
12 The JCVs
can be seen in table 3. Based on this, the revised average
JCV for the Marseilleviridae is 0.55, indicating they make
up a single baramin.

Wiseana iridescent virus and Invertebrate iridescent virus 3

Group 8 is made up of two species, which belong to
the family of Iridoviridae, which is a genetically diverse
group of viruses. Not much is known about the relationship
between these two viruses, but their JCV is significant
enough to group them together.

Ascovirus

Group 9 is made up of three Ascovirus species:
Trichoplusia ni ascovirus 2c (TnAV), Heliothis virescens
ascovirus 3e (HvAV), and Spodoptera frugiperda ascovirus
1a (SfAV). As of date only five Ascovirus species in the
genus Ascovirus have been recognized by the International
Committee on Taxonomy of Viruses,
21 so not much is
known about them. They mainly infect lepidopteran insects,
and induce reorganization of the host cell nucleus, resulting
in lysis.

This group has the lowest average JCV value for all
species pairs, 0.19, therefore these species could probably
form an apobaramin. There were 119 protein sequences
available for a fourth Ascovirus species, Diadromus
pulchellus ascovirus 6a (DpAV), so JCV values were
calculated with the other three species, but were much lower
than 0.19 (0.021, 0.01, and 0.022). This supports the idea
that D. pulchellus may be a member of another Ascovirus
baramin. In fact, Stasiak et al.
22 analyzed the PolIII-PolI
regions of the δ DNA polymerase gene between these four
species, and found that they were very divergent (with a
similarity of less than 50%), thereby separating these four
species into two groups, with TnAV, HvAV, and SfAV in
one group, and DpAV in the other. Interestingly, these
two groups are divided based on how their host organisms
(wasps) infect their prey (caterpillars).

Pandoraviridae

The small group Pandoraviridae is made up of two
species, Pandoravirus salinus, discovered in marine
water near Chile, and Pandoravirus dulcis, discovered
in fresh water near Melbourne. A third species has also
been discovered, P. inopinatum, with a genome size of
2. 24 Mbp. The ORFan content for P. salinus is 84% (its
most abundant virion protein is an ORF), yet only three
species have been discovered, so this intrabaraminic ORFan
gene content may decrease with more newly discovered
Pandoravirus species. However, one characteristic which
separates these species from other NCLDVs is that it lacks
a major capsid protein which is present in all other groups.
23
JCVs were calculated between P. inopinatum and the other
two Pandoravirus species, resulting in a revised average
JCV of 0.32 ± 0.02. It was also found that the average
JCV between Pandoravirus and Pithovirus sibericum is
only 0.008, therefore P. sibericum should be separated into
another baramin. Similarly, an average JCV of 0.04 ± 0.01
was calculated between the three Pandoravirus species
and Mollivirus sibericum, another possible NCLDV species
which is also held to be similar to Pandoraviridae. This low-protein content is similar to results described in Abergel et
al.
23 (89% of 18% of its genes similar to Pandoraviridae).
Due to the low JCVs it is also suggested that this species be
separated from Pandoraviridae.

Other species pairs

Besides the aforementioned 10 virus clusters there
are three pairs of viruses who show a faint similarity
with each other. Paramecium bursaria Chlorella virus
NYs1 and Acanthocystis turfacea Chlorella virus 1 (both
Phycodnaviruses) have a JCV of 0.19, similar to the three
Ascovirus species of group 9. Feldmannia virus and
Ectocarpus siliculosus virus 1 (Phycodnaviruses) have a
JCV of 0.098. According to Park et al.
24, their DNA adenine
methyltransferase proteins have an identity of 36%.

Ranaviruses

A third pair of species, Singapore grouper iridovirus
(SGIV), and Frog virus 3 (FV3) come from the genus

Ranavirus, within the family
Iridoviridae. Their JCV is 0.22. These
viruses infect mainly amphibians
and/or fish.
25 The proteomes for three
other Ranaviruses were downloaded
(Epizootic hematopoietic necrosis virus
(EHNV), Tiger frog virus (TFV) and
Ambystoma tigrinum virus (ATV)),
and compared to the other two virus
proteomes. It was found that with these