We are the in the process of publishing a paper in the ''Journal of Visualized Experiments'' ([http://www.jove.com JoVE]) that describes this protocol in much more detail. The JoVE paper will be an open access paper. If you would like a preprint, contact [[Biomolecular Breadboards:More Info|Richard Murray]].

=== Cost estimate ===

=== Cost estimate ===

Line 36:

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=== Design Cycle Time Analysis ===

=== Design Cycle Time Analysis ===

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For a more detail description, please see [[Biomolecular Breadboards:Protocols:Cycle Time Measurements|Breadboard Cycle Time Measurements]].

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{| border = "1" cellpadding="5" style="width: 800px"

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|-

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! rowspan=2 width=250px | Circuit

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! colspan=4 | Assembly time

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|-

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! ''In vitro'' testing <br>(linear DNA)

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! ''In vitro'' testing <br>(multiple plasmids)

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! ''In vitro'' testing <br>(single plasmid)

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! ''In vivo'' testing <br>(single plasmid)

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|-

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| Negatively autoregulated gene

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| style="text-align:center" | 6.5 hours

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| style="text-align:center" | N/A

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| style="text-align:center" | 3 days

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| style="text-align:center" | 3 days*

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|-

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| Bistable switch

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| style="width: 200px; text-align:center" | 6.5 hours*

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| style="width: 200px; text-align:center" | 3 days

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| style="width: 200px; text-align:center" | 5 days*

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| style="width: 200px; text-align:center" | 5+ days*

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|}

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<nowiki>* Estimated time based on similar constructions. All other times are measured.</nowiki>

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For a more detailed description, please see [[Biomolecular Breadboards:Protocols:Cycle Time Measurements|Breadboard Cycle Time Measurements]].

We are the in the process of publishing a paper in the Journal of Visualized Experiments (JoVE) that describes this protocol in much more detail. The JoVE paper will be an open access paper. If you would like a preprint, contact Richard Murray.

Cost estimate

The cell-free circuit breadboard costs approximately 2.64 cents per uL, or 31.7 cents per 12 uL reaction. The cost distribution is shown in the table below.