SensitivityThe minimum detectable dose of human HS3ST2 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m

Product Description specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HS3ST2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HS3ST2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HS3ST2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HS3ST2 bound in the initial step. The color development is stopped and the intensity of the color is measured.