PMA-induced NETosis of human neutrophils. 2-Photon imaging of NETosis events induced via PMA-stimulation. The extracellular traps are stained with Sytox-Orange, while the activation of neutrophils is indicated via the expression of CD66b on the cell surface. (By Anika Klingberg and Manuel Stecher)

Microglia in the brain of a CX3CR1-GFP mouse. In-situ 2-Photon imaging of a CX3CR1-GFP mouse brain shows GFP+ microglia in the tissue below the meninges (SHG) and the brain vasculature (CD31). (By Anika Klingberg, Publication)

Visualisation of NETs by bicoloured dSTORM. Human Neutrophils were stimulated with PMA for 3 hours at 37°C. Afterwards, the cells were fixed with 4% PFA. Histones H1 and Neutrophil Elastase were stained with antibodies. Consequently the MEA-buffer was added, which brought the fluochromes in a blinking state. (By Alexandra Brenzel and Jacqueline Heinen-Weiler)

In vitro hyphal cell wall staining of the fungus Aspergillus fumigatus.Aspergillus fumigatus tdTomato is stained with the fluorophore coupled antibody JF5 Dylight650 and the nucleus with DAPI. The fungus was grown for 16h after conidia seeding. (By Djamschid Solouk)

Visualisation of NETs via SEM (= Scanning Electron Microscopy). Human Neutrophils were stimulated with PMA and E.coli added. They were incubated for 3 hours at 37°C to induce NETs, which caught the E.coli. Afterwards the image was colored using Photoshop (Adobe). (By Jacqueline Heinen-Weiler)

Visuals » Movies

The surface and the complex vascular organization (CD31) of an entire kidney is shown via optical clipping into the kidney volume. Additionally, both the Bowman's capsule (SHG) and the surrounding kidney tubules (autofluorescence) are shown. Glomerular capillaries of untreated mice are normally shaped, while NTN treated animals exhibit vessel damage.

Autofluorescence of the heart muscle tissue offers detailed structural information about the external and internal organ structure. The vasculature of the heart was specifically stained via CD31. Clipping of the 3D model allows a view inside the right ventricle showing its intracardial papillary muscles and chordae tendinae as well as the ventricular septum.

A few RAW macrophages can be seen here carrying Candida albicans yeasts. A large yeast is taken up by a cell at 30 min and is pulled up towards the cell. The observation was made over a period of 2 h with one frame every 30 s.