Cell Size Estimation

Size Calibration Standards include five highly uniform unlabeled microbead populations of different diameters (~4-11µm), which can be used to construct calibration plots for electronic volume and light scatter instrumentation.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Our flow cytometry customers demand precisely manufactured microparticles as calibration tools. Our unique synthesis methods routinely result in particles with fluorescence CV’s less than 5% and often as low as 1%. This kit guarantees that our discriminating customer will have the finest particles with the lowest fluorescence CV currently available from our stock.

Current applications in flow cytometry extend far beyond traditional lymphocyte immunophenotyping, with some involving the analysis of very small particles such as platelet- and endothelial-derived microparticles or microbial species. Our Small Bead Calibration Kits allow operators to verify the resolution capabilities of the flow cytometer, and to establish appropriate instrument settings for the analyses.

Current applications in flow cytometry extend far beyond traditional lymphocyte immunophenotyping, with some involving the analysis of very small particles such as platelet- and endothelial-derived microparticles or microbial species. Our Small Bead Calibration Kits allow operators to verify the resolution capabilities of the flow cytometer, and to establish appropriate instrument settings for the analyses.

The Nanobead Calibration Kit comprises highly defined microspheres with an internalized fluorescent dye to allow users to determine the capabilities of their cytometer and appropriate instruments settings, as size references for small particle characterization, and assessing small particle handling processes.