Glutathione Peroxidase 1 (GPX1), also known as Cellular Glutathione Peroxidase, is a 201 amino acid , 23 kD member of the glutathione peroxidase antioxidant enzyme family. The Glutathione Peroxidase family protects cell surfaces, extracellular fluid components, and enzymes from oxidative stress by catalysing the reduction of hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX1 is a 92 kD homotetramer consisting of four identical 23 kD subunits, each containing a selenocysteine residue at the active site. GPX1 is an important antioxidant enzyme and protects red blood cells and other cells from oxidative damage. Deficiencies in red cell GPX1 have been associated with hemolytic anemia. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Applications:

Western Blot: An antibody concentration of 1.0ug/ml is recommended.

Catalog #

G8127-11

Storage and Stability

Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Clone Type

Polyclonal

Isotype

IgG

Host

Goat

Source

Human

Concentration

As reported

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.1ml is used, the concentration will be 1mg/ml.

Purity

Purified by immunoaffinity chromatography.

Immunogen

E. coli-derived recombinant human GPX1 (rhGPX1, aa48-201)

Specificity

Detects endogenous human, mouse, and rat GPX1 in Western Blots. Does not crossreact with recombinant human GPX2 or GPX3 in Western Blots.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.