Impact/Purpose:

Description:

The effect of low doses of free chlorine on the detection by qPCR of Helicobacter pylori (H. pylori) cells by qPCR in tap water was monitored. H. pylori target sequences (within suspended, intact cells at densities of 102 to 103 cells /ml) were rendered undetectable by qPCR analysis after 17 hours of continuous exposure to low chlorine levels common to treated drinking water distribution systems. This study suggests that degradation of H. pylori DNA target sequences by chlorine levels commonly found within treated water distribution systems occurs within the average water retention times (2-3 days) commonly found in these systems.

Record Details:

Record Type: DOCUMENT (JOURNAL/PEER REVIEWED JOURNAL)

Product Published Date: 03/01/2011

Record Last Revised: 05/24/2012

OMB Category: Other

Record ID: 239026

Organization:

U.S. ENVIRONMENTAL PROTECTION AGENCY
OFFICE OF RESEARCH AND DEVELOPMENT
NATIONAL EXPOSURE RESEARCH LABORATORY
MICROBIOLOGICAL AND CHEMICAL EXPOSURE ASSESSMENT DIVISION
MICROBIAL EXPOSURE RESEARCH BRANCH