Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

IHC-P

Use at an assay dependent concentration.

WB

Use a concentration of 0.5 µg/ml. Predicted molecular weight: 22 kDa.

IHC-Fr

Use at an assay dependent concentration.

Target

FunctionAccessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Anti-NDUFB8 antibody [20E9DH10C12] images

Skeletal muscle immunohistochemistry using ab110242 on frozen tissue sections from a patient with a single large deletion of the mtDNA show a mosaic of complex I positive and complex I negative fibers.

Predicted band size : 22 kDaExtra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.

I now had a look at the homology between the immunogens of the antibodies included in the MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail (ab110413) and the respective proteins in Drosophila. Read More

Thank you for this additional information.
I can confirm that ab110413 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail) contains 5 antibodies.
The datasheets and more information about these antibodies can be found when following...