It is a lot of centrifugation steps which probably accounts for the fact that I have very few cells during FACS analysis, has anyone any other protocol that could enable recovering more cells ? (I'd like to add that I carefully remove the sup after each centrifugation step)

Many thanks

-julnobody-

Are there cells smeared against the wall of your reaction tube after centrifugation ?

-Tabaluga-

Not that I can see but my pellet is usually hardly visible

-julnobody-

Is the pellet hardly visible from the start ? Because 80 000 cells is already a low number to start with, in my opinion.
Your protocol looks OK to me.