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Hi there, I have to do a cell wall extraction on bacteria soon and my protein of interest is cell wall anchored. My supervisor told me to do TCA precipitation (haven't done before) but I assume it's too isolate the proteins within the cell wall?? He told me I would have three components by the ...

Aha ok. Thank you very much, I just received a mail from my teacher and he actually suggested I do the precipitation, just as you did. From one thing to another though. What is the purpose of a bacteriophage promoter being ligated in to a vector along with your gene? Is it ...

... buffer (lyses buffer), a very viscous, mucus like substance results. If I carry on the protocol as recomended by Quiagen and add the AP2 buffer (precipitation buffer), centrifuge and after which I atempt to recover the supernatant, in several cases there is no supernatant to recovery. Just a ...

... movement of resources within tropical forest ecosystem. The ecologist is monitoring the processes of evaporation, condensation, transpiration, and precipitation over a 180 day period. Which of the following cycles is the ecologist studying? water cycle. Correct. I hope this helps you at least a ...

... movement of resources within tropical forest ecosystem. The ecologist is monitoring the processes of evaporation, condensation, transpiration, and precipitation over a 180 day period. Which of the following cycles is the ecologist studying? water cycle.