NeXtal Evolution µplates are used for automated or manual setup of protein crystallization trials. Their excellent optical properties, UV transparency, and low birefringence make them suitable for automatic visualization systems. They are supplied as 96-well microplates, where each position comprises a reservoir well, with 60–100 µl screening solution, and a protein well, which can hold 0.1–2 µl protein solution plus 0.1–2 µl reservoir solution.

NeXtal Evolution µplates offer a platform for high-throughput screening of crystallization conditions. Direct solution transfer can be made from NeXtal DWBlocks, which share the same format. Equal volumes of protein and crystallization solution are mixed in the protein wells and the plate is sealed with a tape sheet. Their innovative design ensures improved drop centering and easy crystal fishing (see figure "Important technical features").The wide rims minimize evaporation during crystallization experiments (see figure "Comparison of evaporation"). In addition, the rims feature a cutter path, which allows opening of a well without disturbing the neighboring experiments.

Features

Well shape

Semielliptical

Number of wells per reservoir

1

Crystallization well volume

0.1–4 µl

Reservoir volume

60–100 µl

Applications

The NeXtal Evolution µplate is highly suited for automated or manual setup of protein crystallization trials and is compatible with the PX Scanner.