... specimens from 16 enterovirus-infected newborn infants, and it remained more sensitive throughout their illnesses, with a combined sensitivity of serum and urine PCR of 88% and a specificity of 100% =-=(6)-=-. (iii) Impact on hospitalization and patient management. Enterovirus PCR has had a profound impact on the management of patients with aseptic meningitis (217). In a large study in 1998, a retrospecti...

...3, 16, 20, 23, 30, 32, 39, 42). It has also been successfully used to detect enteroviruses in serum and throat swabs and, with somewhat reduced sensitivity compared to that of virus culture, in urine =-=(2, 3, 28, 34)-=-. By evaluating the Enterovirus Amplicor test alongside in-house PCR methods in a multicenter study, we now provide further evidence of the utility of this assay. The major problem identified in this ...

... emerging serotypes; it should also be noted that building a complete panel of monoclonal antibodies against specific viral strains is not easy and can be expensive. More recent, molecular approaches =-=(1, 5, 9, 10, 11, 21, 23, 24)-=- have been developed and successfully used to detect most of the EVs, including nontypeable isolates and EVs which cannot readily be propagated in cell culture. In this study we compare immunological ...