Microscale Thermophoresis

Monolith NT.115 / NT.LabelFree (NanoTemper Technologies)

Location:

Research team of Dr. Michael Mareš

Responsible person:

Jaroslav Srp (phone 220 183 470)

The microscale thermophoresis (NanoTemper Technologies, Münich, Germany) is a highly sensitive technique based on the Soret effect that enables the quantitative analysis of
molecular interactions in solution at the microliter scale. Binding constants can be determined e.g. for protein-protein, protein-DNA and protein-small molecule interactions.
Two MST instruments are available: Monolith NT.115 requires a fluorescently labeled molecule in the analyzed system, while Monolith NT.LabelFree utilizes the intrinsic tryptophan
fluorescence of native proteins.