KHUANG at CHEMVX.TAMU.EDU wrote:
>> Hi, Netters,
> I am trying to do some site-directed mutagenesis by PCR, <snip>
> Does anybody have used "QuickChange Kit(stratagene)" to mutagenese more than 8kbplasmids?
> Any other suggestion?
>> Thanks!!
>> Huang
Hi,
I haven't used the Quikchange kit on a plasmid greater that 8kb but I have
used it on one of about 8kb with no problems at all. As long as your
extention time is increased and you allow enough dNTP's, I don't see why
they'd be a problem.
Good luck,
Lynne
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