* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications. ** IMAC and GST cartridges are designed for use with the Profinia Protein Purification System. *** Column volume.

Bio-Scale Mini Affi-Gel Blue cartridges are 5 ml Bio-Scale Mini cartridges filled with 100–200 mesh Affi-Gel Blue support for use in affinity chromatography. This form of Affi-Gel Blue gel is a crosslinked agarose bead with covalently attached cibacron blue F3GA dye that is ideally suited for albumin removal. Proteins and peptides are bound and released with a high degree of specificity by manipulating the composition of eluent buffers.

Bio-Scale Mini cartridges have a patented* double-wall cartridge design that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts.

Bio-Scale Mini cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system; simple luer fittings make connections easy. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems. Bio-Scale Mini affinity cartridges can also be run on the Profinia protein purification system.

* U.S. patent 7,208,087.

Size

5 ml bed volume

Dimensions (L x ID)*

40 x 12.6 mm

Maximum pressure tolerance

10 psi (680 mbar, 0.26mPa)**

Operational flow rates

0.5–2.0 ml/min (25–100 cm/hr)

Fittings

Female luer fitting inlet; male luer fitting outlet

Column material

Polypropylene

Frit material

Polyethylene (HDPE)

Shipping conditional

0.02 M sodium phosphate, pH 7.4, 0.05% NaN3

Storage recommendations

0.02 M sodium phosphate, pH 7.4, 0.05% NaN3

Autoclavability

Not autoclavable

* L, length; ID, inner diameter.

** Although the pressure limitation of the cartridge is 45 psi, the agarose gel tends to compress above 10 psi (generally 3.0 ml/min).

What is the education discount policy?

For more than 15 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows high school and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices.

How do I apply for the education discount?

If you are an educator at the high school or college level, visit our Education Discount Policy page to establish an education account number.

If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the list price. If the problem persists, please call customer service at
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