One of my recombinant His-tagged protozoen protein (45kDa), expressed in
E. coli, does not work in western transfers, perhaps because it has an
isoelectric point of 9,6. I do semi dry transfers and tried several
transfer buffers, but the results were disappointing. The protein was
transfered less than 50%. Does anybody have an idea, how to blot a basic
protein like this?
Thanks a lot
Andrea
Andrea Irion, Swiss Tropical Institute, Socinstr. 57, CH-4002 Basel
IRION at ubaclu.unibas.ch