What is the Xfect Protein Transfection Reagent and how does it work?

Xfect Protein Transfection Reagent is a modified peptide with cell-penetrating activity whose amino acid composition enables it to interact with a protein cargo and transport this protein across a cell membrane barrier. Just incubate your protein of interest with Xfect in the supplied buffer for 30 min, then apply the mixture to your cells—and 2 hr later, you are ready to assay for protein activity.

What are the advantages of Xfect Transfection Reagent compared to other protein delivery technologies?

The two technologies most frequently used for protein delivery are based on lipids and cell-penetrating peptides. Lipid-based reagents tend to demonstrate high cytotoxicity, while peptide-based reagents transfect with low efficiency. Xfect Protein Transfection Reagent offers the best advantages of both, i.e., it retains low cytotoxicity and delivers more protein to a higher percentage of target cells (see Images & Data tab). Moreover, Xfect Protein can transfect cells that are growing at a higher density than competing products, which is important because assays are often performed within a few hours post-transfection and the higher cell densities ensure sufficient material for downstream analysis.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.