Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

large cell lymphoma; diffuse histiocytic lymphoma

Age

10 years

Gender

Male

Ethnicity

Caucasian

Applications

The availability of a permanently established human neoplastic cell line as the source of this type C RNA virus should greatly facilitate studies of its biological significance. Evidence has recently been obtained that the virus can induce proliferation of normal human monocytes and histiocytes in vitro.

The cells are surface Ig negative (sIG-).
The cells are non-specific esterase, acid phosphatase and oil red O positive.
The cells are periodic acid Schiff negative.
These cells phagocytose Candida albicans and latex particles.
The cells are reported to be very weakly E - rosette positive.
ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Cultures can be maintained by the addition of fresh medium. An inoculum of 8.0 x 104 to 2.0 x 105cells/mL is recommended. Subculture when the cell concentration is between 1.0 x 106 and 1.5 x 106 cells/mL. Subcultivation ratio: A subcultivation ratio of 1:4 to 1:12 is recommended.Interval: As needed.Medium renewal: every 2 to 4 days

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.