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Toxic nests are placed as a calldown ability from Abathur's top menu. Abathur can store up to 5 of them at once, creating a new one every 20 seconds if there are slots available. They can be placed anywhere the player has vision to.

They explode (hotkey X) to deal 125 damage to enemies when they pass. The explosion can be set to autocast, or manually triggered. Toxic nests also provide creep.