Colony Forming Cell (CFC) Assay

In vitro colony-forming cell (CFC) assay is often utilized in the evaluation and quantification of multipotential and lineage-restricted myeloid, erythroid, megakaryocytic progenitors, as well as pre-B-lymphoid cells in animal models, which require sterile preparation of cells to prevent unwanted contamination of these long-term cultures. Since 2010, Creative Animodel has performed this assay for world-wide pharmaceutical companies, government and research institutes to study human hematopoiesis. We can also provide customized services for individualized studies in preclinical drug development.

Methodology of CFC assay：
● Cells are plated in semi-solid medium
● Progenitor cells proliferate and differentiate during the culture period (up to two weeks)
● Distinct colonies grow from individual progenitor cells in the semi-solid medium
● Colonies are classified and counted to determine total number of differentiated colonies of each cell lineage

Why choose Creative Animodel?
● Reliable quality: We have built a reputation by providing high-quality results to meet every specific requirement of clients
● Efficient communication: We place a priority on good communication and prompt discussion with clients throughout our studies.
● Extensive expertise in stem cell biology: We possess a professional scientific team owning extensive knowledge in stem cell biology and the hematopoietic CFC assay, that guarantee high-standard products for clients.

To learn a more in-depth knowledge on CFC assay, please feel free to contact us.