The conditional KO allele has been generated after Flp-mediated recombination by crossing chimeras to a Flp-Deleter on a C57BL/6 background.

The conditional KO line has been derived using C57BL/6NTac animals and the Flp-transgene was removed by segregation.

The constitutive KO allele can be generated by Cre-mediated recombination. Deletion of exon 7 should result in loss of function of the Stk33 gene by deleting part of the catalytic domain and by generating a frameshift from exon 6 to exon 8 (premature Stop codon in exon 8).

The remaining recombination sites are located in non-conserved regions of the genome.