Datasheet Extract

L-Lactic Dehydrogenase, LDH-1(H4)/LDH-2(H3M) isoenzyme, is isolated and purified from Human placenta.Freund’s complete adjuvant is used in the first step of the immunization

Application

This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice.Working Dilutions:Non-precipitating antibody-binding techniques: Up to 1/2,000.

Concentration

10.0 mg/ml

Storage

Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.If a slight precipitation occurs upon storage, this should be removed by centrifugation.Shelf life: one year from despatch.

Format

Label:

Biotin

Molar radio:

Biotin/IgG~ 6.9

Purification:

Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Buffer System:

PBS, pH 7.2 without preservatives and foreign proteins

State:

Lyophilized hyperimmune IgG fraction

Reconstitution:

Restore by adding 1.0 ml of sterile distilled water

Specificity

Specificity:

The antibody recognizes LDH-1(H4)/LDH-2(H3M) isoenzyme, is isolated and purified from Human placenta.The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition.Cross-reactivities against enzymes of other sources may occur but have not been determined.