RNA Master Mix Sets

Each set contains enzymes and buffers that are ideally suited for library preparation for next generation sequencing, and for preparation of expression libraries. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.

Sets are also available for the preparation of small RNA for the following platforms:

Each NEBNext® Small RNA Library Prep Set contains adaptors, primers, enzymes and buffers that are ideally suited to convert small RNA transcripts into cDNA libraries (barcoded libraries for multiplex Illumina) for next generation sequencing on the Illumina platform . Each of these components must pass rigorous quality control standards and is lot controlled, both individually and as a set of reagents.

Illumina® is a registered trademark of Illumina, Inc.
454™ is a trademark of Roche.
SOLiD™ is a trademark owned by Life Technologies, Inc.

Videos

The common problem of adaptor dimer formation during small RNA library construction can be avoided by using NEBNext® protocols. Learn about this technique, and how it improves both performance and sensitivity in library construction.

Advantages of NEBNext Reagents for RNA Library Preparation

Convenient Formats – Enzymes, buffers and nucleotides are included, at appropriate concentrations and in appropriate volumes, and are available in set or master mix format. Large and small sizes are available.

Functional Validation – Each set is validated together by preparation of a library from an accepted RNA reference sample, followed by sequencing on the appropriate sequencing platform (Illumina, 454 or SOLiD).