The present invention relates to the use of inhibitors or blockers of Ih (hyperpolarization-activated cationic current) channels in the treatment of cognitive disorders. In preferred aspects of the present invention, an effective amount of a compound is administered to a patient in need, wherein the compound has the chemical structure: Where R1 is H, or an optionally substituted C1-C3 alkyl, preferably a C2 alkyl (ethyl) group; R2 is an optionally substituted C1-C3 alkyl group, preferably a methyl group; R3 is H, an optionally substituted C1-C3 alkyl (preferably methyl), a halogen or 0(Ci-Ca) alkyl; R4 is an optionally substituted C1-C6 alkyl, C(O)—(C1-C5)alkyl, C(O)-aryl, C(O)O—(C1-C4)alkyl, C(O)O-aryl, or an optionally substituted heterocyclic, aryl or heteroaryl group; R4 is H or an optionally substituted C1-C6 (preferably a C1-C3) alkyl; R5, R6 and R7 are each independently H, halogen, an optionally substituted C1-C6 alkyl (preferably, an optionally substituted C1-C3 alkyl), 0-(C1-C3) alkyl, or an optionally substituted heterocyclic, aryl or heteroaryl group; Y− is an anion of a pharmaceutically acceptable salt (a physiologically acceptable anion, preferably a Cl−, Br−, I−, OAc−); or a solvate or polymorph thereof, optionally, in combination with guanfacine and/or chelerythrine, and a pharmaceutically acceptable carrier, additive or excipient to a patient in need of therapy.

1. A method of treating a cognitive or mood disorder in a patient comprising administering to said patient an effective amount of a compound which is a blocker or inhibitor of Ih channels in said patient and optionally, an effective amount of guanfacine or its pharmaceutically acceptable salt form and/or chelerythrine in its neutral or salt form.

2. The method according to claim 1 wherein said Ih channels are found in the prefrontal cortex of said patient and comprise a HCN1/HCN2 heteromer.

3. The method according to claim 1 wherein said cognitive disorder is a memory or learning disorder.

35. A pharmaceutical composition comprising an effective amount of a compound according to claim 33 in combination with a pharmaceutically acceptable carrier, additive or excipient.

36. A pharmaceutical composition comprising an effective amount of a compound according to claim 34 in combination with a pharmaceutically acceptable carrier, additive or excipient.

37. A pharmaceutical composition comprising an effective amount of a compound according to claim 33 in combination with an effective amount of guanfacine or its pharmaceutically acceptable salt and a pharmaceutically acceptable carrier, additive or excipient.

38. A pharmaceutical composition comprising an effective amount of a compound according to claim 33 in combination with an effective amount of chelerythrine in its neutral or salt form and a pharmaceutically acceptable carrier, additive or excipient.

39. A pharmaceutical composition comprising an effective amount of a compound according to claim 33 in combination with an effective amount of guanfacine or its pharmaceutically acceptable salt form, chelerythrine in its neutral or salt form and a pharmaceutically acceptable carrier, additive or excipient.

40. A method of treating the loss of function in patients from cAMP opening of Ih (HCN) channels and prefrontal cortical impairment (PFC) comprising administering to said patient an effective amount of an Ih channel blocker or inhibitor.

41. The method according to claim 40 wherein said loss results in a mood disorder or schizophrenia.

Neuropsychological studies first identified marked impairments in ADHD patients performing tasks requiring PFC function, and imaging studies confirmed both structural and functional insufficiencies in PFC circuits. Attention-deficit/hyperactivity disorder patients also show evidence of genetic alterations, including genes related to catecholamine neurotransmission. See, Arnsten and Li, Biol. Psychiatry, 57, 1377-1384 (2005). PFC deficits have also been observed in a number of other neuropsychiatric disorders (e.g. schizophrenia, bipolar disorder, Posttraumatic Stress Disorder, Anxiety disorders, Tourettes Syndrome), in normal aging, in neurodegenerative disorders such as Alzheimer's and Parkinson's Disease, and following traumatic brain injury to the PFC.

The PFC subserves working memory. Working memory is the ability to bring to mind an event from long term storage, or keep in mind an event that has just occurred, and retain this information in a temporary buffer in order to guide behavior and thought. Given the short term nature of working memory, it cannot involve architectural changes such as structural changes in synapses, as is thought to occur with long term memory consolidation. Rather, working memory is thought to arise from a network of PFC neurons with shared properties, engaged in recurrent excitation. The spatial working memory characteristics of neurons in the primate PFC (area 46) have been well characterized in monkeys performing spatial working memory tasks that require the animal to remember a visually cued spatial location over a brief delay period. The cued position continuously changes, requiring constant updating of spatial working memory. Goldman-Rakic, Neuron 14, 477-485 (1995) identified PFC microcircuits with spatially tuned mnemonic activity during the delay period: pyramidal neurons with similar spatial tuning properties mutually exciting each other, and those with dissimilar spatial properties inhibiting each other via GABAergic interneurons. The horizontal connectivity of layer III pyramidal cells is thought to provide the anatomical basis for PFC microcircuits (Kritzer, J Comp Neurol., 359:131-143, 1995 and Goldman-Rakic, Neuron 14, 477-485 (1995)). These connections allow PFC neurons to continue firing during the delay period when no stimulus is available in the environment, maintaining representations over time even in the presence of distracting stimuli (Miller, et al., J. Neurosci. 16:5154-5167, 1996). This is a fragile process that is highly dependent on the correct neurochemical environment.

FIG. 2 shows the effects of prefrontal infusions of the α2A agonist guanfacine (GFC; 0.0001 μg) on spatial working memory performance in aged rats (n=9). Results represent mean+/−SEM percent correct on the delayed alternation task Co-infusion of 0.21 mmol Sp-cAMPS (Sp) reversed the effects of guanfacine. ** significantly different than saline (SAL); † significantly different than guanfacine alone. Aged animals are particularly sensitive to the enhancing effects of guanfacine and thus were the focus of this study.

FIG. 3 shows the paradigm used for electrophysiological recordings in monkeys performing a WM task (A) ODR task: Trials began when the monkey fixated on a central point for 0.5 sec. A cue was present in 1 of 8 possible locations for 0.5 sec and was followed by a delay period of 2.5 sec. When the fixation point was extinguished, the monkey made a saccade to the location of the remembered cue. (B) Position of the cylinder (big circle) and the region of electrophysiological recording in dorsolateral PFC (red area). PS: principal sulcus; AS: arcuate sulcus. (C) Neuronal activity of a PFC cell on the ODR task. Rasters and histograms are arranged to indicate the location of the corresponding cue. This cell exhibited significant delay-related activity for the 180° location (preferred direction) but not for other directions (e.g. 0°, nonpreferred direction).

FIG. 4 shows the effects of β2A-AR stimulation and blockade on spatially tuned delay-related firing of PFC neurons in monkeys performing a spatial WM task. (A). Iontophoretic application of the β2A-AR agonist, guanfacine, enhanced spatially tuned, delay-related firing for a neuron with weak tuning under control conditions. Rasters and average histograms of neuron H636 during the control condition vs. guanfacine iontophoresis for preferred and nonpreferred directions are shown. (B) Iontophoresis of a low dose of guanfacine increased delay-related firing in a well-tuned neuron as well, while a high dose was without effect. (C) Iontophoresis of the β2-AR antagonist, yohimbine, decreased delay-related firing and eroded spatial tuning of a PFC neuron. (D) Co-iontophoresis of yohimbine with guanfacine reversed the enhancing effects of guanfacine on delay-related firing. Average histograms of neuron H653 during the control condition, guanfacine iontophoresis, and guanfacine/yohimbine co-iontophoresis for preferred and nonpreferred directions are shown. (E) Iontophoretic application of guanfacine (red) significantly enhanced spatially tuned delay related activity at the population level (35 neurons). (F) Suppressive effect of yohimbine (red) on spatial delay-related activity at the population level (15 neurons).

FIG. 6 shows the effects of the HCN channel blocker ZD7288 on the spatial WM-related firing and network connectivity of PFC neurons. (A) Iontophoresis of a low dose of ZD7288 increased delay-related firing in a well-tuned neuron. (B) Iontophoretic application of ZD7288 caused a dose-dependent effect on spatially tuned delay-related firing in a neuron with weak tuning under control conditions. ZD7288 at 5 nA and 10 nA enhanced spatially tuned delay-related activity, while a high dose (40 nA) eroded the spatial tuning. (C)Co-iontophoresis of ZD7288 with yohimbine reversed the suppressive effects of yohimbine on delay-related firing. (D) Co-iontophoresis of ZD7288 with etazolate reversed the suppressive effects of etazolate on delay-related firing. (E) Iontophoretic application of ZD7288 (red) significantly enhanced spatially tuned delay related activity at the population level (27 neurons). (F) Co-iontophoresis of ZD7288 with etazolate (green) reversed the suppressing effects of etazolate (red) on delay-related activity at the population level (8 neurons). (G) Bath application of ZD-7288 resulted in a prolongation of the persistent activity of the Up state in ferret PFC cortical slice in vitro as recorded simultaneously with extracellular multiple unit (MU; upper traces) and intracellular recording from layer 5 neurons (lower traces). This effect of ZD7288 was associated with a hyperpolarization of pyramidal neurons and a strengthening of the synaptic barrages mediating the recurrent network activity.

FIG. 8 shows the expression and co-expression patterns of HCN channels (double arrowheads) and α2A-ARs (arrowheads) in dendritic spines of primate PFC. HCN (A, B) and α2A-AR (C) immunoparticles mark extrasynaptic membranes in the spine head and neck region (compare B to C); curved arrows point to emerging spines. In dual labeling, α2A-AR was visualizd with immunoperoxidase and HCN1 with silver-enhanced nanogold (C) or with reversal of the immunocytochemical sequence (E). Sequential gold-enhancement of nanogold is shown in (F); (G) depicts the reverse procedure as in (E).

BRIEF DESCRIPTION OF THE INVENTION

The present invention relates to a number of compounds which may be used to modulate Ih channels, preferably including blocking or inhibiting Ih channels to strengthen the connectivity of PFC microcircuits or neurons (prefrontal cortical function) to treat impairment of cognitive function in a patient. Applicants have discovered in animals studies that a compound which functions as an Ih channel blocker may be used to enhance prefrontal cortical function and to be useful to treat cognitive deficiencies, the impairment of cognitive function or to enhance cognitive function in a human, including memory disorders/deficiencies and learning disorders/deficiencies, which may result from aging, trauma, stroke, neurotoxic agents, neurodegenerative disorders or anxiety disorders, including those which are associated with drug-induced states, neurotoxic agents, Alzheimer's disease, and aging. These conditions are readily recognized and diagnosed by those of ordinary skill in the art and treated by administering to the patient an effective amount of one or more compounds according to the present invention. The present invention is also useful for the treatment of memory disorders/deficiencies and/or learning disorders/deficiencies which may be associated with such conditions as attention deficit disorder (ADD), attention deficit disorder with hyperactivity (ADD-HD), autism, and pervasive development disorder (PDD, including PDD-NOS).

In addition, Ih inhibitors or blockers according to the present invention may be used to treat the loss of function in patients from cAMP opening of Ih (HCN) channels and prefrontal cortical impairment (PFC), which are implicated in schizophrenia or certain mood disorders such as depressive disorders. These inhibitors or blockers therefore may be used to treat schizophrenia, and mood disorders, including bipolar disorder, unipolar disorder, dysthymic disorder, post-partum depression, seasonal affective disorder and depression (major depression or major depressive disorder).

Thus, in the present invention, a method of treating cognitive disorders in a patient in thereof comprises administering an effective amount of an inhibitor or blocker of an 1 h channel in said patient. In preferred aspects of the present invention, the Ih channel is HCN1, HCN2 or a heteromer of HCN1 or HCN2. A particularly preferred target of the blockers or inhibitors of the present invention is the Ih channel comprising a heteromer of HCN1 and HCN2.

In certain preferred aspects, the present invention relates to a compound or method using said compound comprising administering an effective amount of a compound according to the structure:

In certain preferred aspects of the present invention, the compound to be used in the present methods is ZD7288, represented by the formula presented hereinbelow.

Additional aspects of the present invention relate to the administration of an Ih channel blocker according to the present invention in combination therapy, for example, with an effective amount of another agent, such as an agent which inhibits cAMP such as guanfacine (N-(diaminomethylidene)-2-(2,6-dichlorophenyl)acetamide) or its pharmaceutically acceptable salt and/or chelerythrine, in its neutral or salt form (which includes all pharmaceutically acceptable salt forms, including the naturally occurring chloride salt form), preferably chelerythrine chloride, in order to potentiate the activity of ZD7288 or one of its analogs or derivatives, thereof.

DETAILED DESCRIPTION OF THE INVENTION

As used herein, the following terms have the following respective meanings. Other terms that are used to describe the present invention have the same definitions as those generally used by those skilled in the art. Specific examples recited in any definition are not intended to be limiting in any way.

The term “patient” or “subject” refers to a mammal, preferably a human to which one or more of the present methods is applied.

The term “effective” is used in context, to describe an amount of a compound or compound, a component or components or a substance or substances which produce a result intended from the use of that compound(s), component(s) or substance(s).

The term “compound”, as used herein, unless otherwise indicated, refers to any specific chemical compound disclosed herein. The compounds of the present invention include all stereoisomers (i.e, cis and trans isomers), tautomers, and all optical isomers of the present compound and related analogs in context (eg., R and S enantiomers), as well as racemic, diastereomeric and other mixtures of such isomers, as well as all solvates and polymorphs of the compounds.

In aspects of the present invention, compounds according to the present invention block or inhibit L channels, including any one or more of the HCN subunits 1-4, preferably HCN 1 and/or 2 and in particular, the HCN 1 & 2 heteromer (HCN1/HCN2 heteromer Ih channel) as described by Chen, et al., Gem Physiol., 117, 491-503 (May, 2001). HCN 1 and 2 are found in the prefrontal cortex (PFC) and the hippocampus and form a heteromer there. The HCN1/HCN2 heteromer Ih channel is the preferred target for blockers to effect treatment of cognitive disorders according to the present invention.

The term “Ih channel blocker” “Ih channel inhibitor”, “blocker of Ih channels” or “inhibitor of Ih channels” are used interchangeably throughout the present application to describe compounds which directly inhibit or block at least a significant part of the function of Ih channels to generate hyperpolarization-activated cation currents. The term “direct” means that the action of the blocker or inhibitor according to the present invention is directly on the Ih channel, rather than inhibition at another site, which indirectly may cause the release of one or more substances which cause inhibition. By reducing or preventing these cation currents from developing, it is shown that Ih channel blockers may be used to treat cognitive disorders by enhancing cognitive functions such as memory or learning, among others.

“Alkyl” refers to a monovalent hydrocarbon radical, preferably a folly saturated hydrocarbon group, containing carbon and hydrogen which may be a straight chain, branched, or cyclic. Examples of alkyl groups are methyl, ethyl, n-butyl, n-heptyl, isopropyl, 2-methylpropyl, cyclopropyl, cyclopropylmethyl, cyclobutyl, cyclopentyl, cyclopentylethyl and cyclohexyl. “Cycloalkyl” groups refer to cyclic alkyl groups such as cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. C1-C6 alkyl groups are preferably used in the present invention. The term “alkyl” also refers to unsaturated alkyls, containing one or more unsaturated groups, within context, and includes alkenyl and alkynyl groups as described hereinbelow, distinguished from aromatic groups, which are fully unsaturated.

The term “substituted” shall mean, within context, a group (substitution group) which is added to a moiety within a compound, e.g. “an optionally substituted alkyl group”. Pursuant to the present invention, within context, the substitution group can be alkyl or alkylene groups containing from 1 to 6 carbon atoms, preferably a lower alkyl containing 1-3 carbon atoms, aryl, substituted aryl, acyl, halogen (i.e., alkyl halos, e.g., CF3), hydroxy, alkoxy, alkoxyalkyl, amino, alkyl and dialkyl amino, acylamino, acyloxy, aryloxy, aryloxyalkyl, carboxyakyl, carboxamido, thio, thioethers, both saturated and unsaturated cyclic hydrocarbons, heterocycles and the like

“Substituted alkyl” refers to alkyls (including unsaturated alkyls) as above-described which include one or more substituted groups such an alkyl or alkylene groups containing from 1 to 6 carbon atoms, preferably a lower alkyl containing 1-3 carbon atoms, aryl, substituted aryl, acyl, halogen (i.e., alkyl halos, e.g., CF3), hydroxy, alkoxy, alkoxyalkyl, amino, alkyl and dialkyl amino, acylamino, acyloxy, aryloxy, aryloxyalkyl, carboxyalkyl, carboxamido, thio, thioethers, both saturated and unsaturated cyclic hydrocarbons, heterocycles and the like. The term “substituted cycloalkyl” has essentially the same definition as and is subsumed under the term “substituted alkyl” for purposes of describing the present invention.

“Aryl” refers to a substituted or unsubstituted monovalent aromatic radical having a single ring (e.g., phenyl) or multiple condensed rings (e.g., naphthyl). Other examples include heterocyclic aromatic ring groups having one or more nitrogen, oxygen, or sulfur atoms in the ring, such as imidazolyl, furyl, pyrrolyl, pyridyl, thienyl and indolyl, among others. Therefore, “aryl” as used herein includes “heteroaryls” having a mono- or polycyclic ring system which contains 1 to 15 carbon atoms and 1 to 4 heteroatoms, and in which at least one ring of the ring system is aromatic. Heteroatoms are sulfur, nitrogen or oxygen.

“Substituted aryl” refers to an aryl as just described that contains one or more functional groups such as lower alkyl acyl, aryl, halogen, alkylhalos (e.g., CF3), hydroxy, alkoxy, alkoxyalkyl, amino, alkyl and dialkyl amino, acylamino, acyloxy, aryloxy, aryloxyalkyl, carboxyalkyl, carboxamido, thio, thioethers, both saturated and unsaturated cyclic hydrocarbons, heterocycles and the like.

“Halo” and “halogen” are used in the conventional sense to refer to a chloro, bromo, fluoro or iodo substituent. The terms “haloalkyl,” “haloalkenyl” or “haloalkynyl” (or “halogenated alkyl”) refers to an alkyl, alkenyl or alkynyl group, respectively, in which at least one of the hydrogen atoms in the group has been replaced with a halogen atom.

“Heterocycle” or “heterocyclic” refers to a carbocylic ring wherein one or more carbon atoms have been replaced with one or more heteroatoms such as nitrogen, oxygen or sulfur. A substitutable nitrogen on an aromatic or non-aromatic heterocyclic ring may be optionally substituted. The heteroatoms N or S may also exist in oxidized form such as NO, SO and SO2. Examples of heterocycles include, but are not limited to, piperidine, pyrrolidine, morpholine, thiomorpholine, piperazine, tetrahydrofuran, tetrahydropyran, 2-pyrrolidinone, δ-velerolactam, δ-velerolactone and 2-ketopiperazine, among numerous others.

“Heteroatom-containing” refers to a molecule or molecular fragment in which one or more carbon atoms is replaced with an atom other carbon, e.g., nitrogen, oxygen, sulfur, phosphorus or silicon. “Substituted heterocycle” refers to a heterocycle as just described that contains one or more functional groups such as lower alkyl, acyl, aryl, cyano, halogen, hydroxy, alkoxy, alkoxyalkyl, amino, alkyl and dialkyl amino, acylamino, acyloxy, aryloxy, aryloxyalkyl, carboxyalkyl, carboxamido, thio, thioethers, both saturated and unsaturated cyclic hydrocarbons, heterocycles and the like. In other instances where the term “substituted” is used, the substituents which fall under this definition may be readily gleaned from the other definitions of substituents which are presented in the specification as well the circumstances under which such substituents occur in a given chemical compound. One having ordinary skill in the art will recognize that the maximum number of heteroatoms in a stable, chemically feasible heterocyclic ring, whether it is aromatic or non-aromatic, is determined by the size of the ring, degree of unsaturation, and valence of the heteroatoms. In general, a heterocyclic ring may have one to four heteroatoms so long as the heterocyclic ring is chemically feasible and stable.

“Isostere” refers to compounds that have substantially similar physical properties as a result of having substantially similar electron arrangements.

“Amine” refers to aliphatic amines, aromatic amines (e.g., aniline), saturated heterocyclic amines (e.g., piperidine), and substituted derivatives such as an ably morpoline. “Amine” as used herein includes nitrogen-containing aromatic heterocyclic compounds such as pyridine or purine.

“Aralkyl” refers to an alkyl group with an aryl substituent, and the term “aralkylene” refers to an alkenyl group with an aryl substituent. The term “alkaryl” refers to an aryl group that has an alkyl substituent, and the term “alkarylene” refers to an arylene group with an alkyl substituent. The term “arylene” refers to the diradical derived from aryl (including substituted aryl) as exemplified by 1,2-phenylene, 1,3-phenylene, 1,4-phenylene, 1,2-naphthylene and the like.

“Alkenyl” refers to a branched or unbranched hydrocarbon group typically although not necessarily containing from 2 to about 12 carbon atoms and at least one double bond, such as ethenyl, n-propenyl, isopropenyl, n-butenyl, isobutenyl, octenyl, decenyl, and the like. Generally, although again not necessarily, alkenyl groups herein contain 2 to about 12 carbon atoms. The term “lower alkenyl” intends an alkenyl group of two to six carbon atoms, preferably two to four carbon atoms.

“Substituted alkenyl” refers to alkenyl substituted with one or more substituent groups, and the terms “heteroatom-containing alkenyl” and “heteroalkenyl” refer to alkenyl in which at least one carbon atom is replaced with a heteroatom.

“Alkynyl” as used herein is subsumed under the term alkyl and refers to a branched or unbranched hydrocarbon group typically although not necessarily containing 2 to about 12 carbon atoms and at least one triple bond, such as ethynyl, n-propynyl, isopropynyl, n-butynyl, isobutynyl, octynyl, decynyl, and the like. Generally, although again not necessarily, alkynyl groups herein contain 2 to about 12 carbon atoms. The term “lower alkynyl” intends an alkynyl group of two to six carbon atoms, preferably three or four carbon atoms. “Substituted alkynyl” refers to alkynyl substituted with one or more substituent groups, and the terms “heteroatom-containing alkynyl” and “heteroalkynyl” refer to alkynyl in which at least one carbon atom is replaced with a heteroatom.

“Alkoxy” as used herein refers to an alkyl group bound through an ether linkage; that is, an “alkoxy” group may be represented as —O-alkyl where alkyl is as defined above. A “lower alkoxy” group intends an alkoxy group containing one to six, more preferably one to four, carbon atoms.

The term “coadministration”, “coadministered” or “combination therapy” is used to describe a therapy in which at least two active compounds in effective amounts are used to enhance cognitive function or to treat a cognitive deficit using an Ih blocker or inhibitor in combination with guanfacine or its pharmaceutically acceptable salt or chelerythrine (as its neutral or salt form) as otherwise described herein at the same time. Although the term coadministration preferably includes the administration of two active compounds to the patient at the same tire, it is not necessary that the compounds be administered to the patient at the same time, although effective amounts of the individual compounds will be present in the patient at the same time.

The term “cognitive function” is used to describe an endeavor or process by a patient or subject that involves thought or knowing. The diverse functions of the association cortices of the parietal, temporal and frontal lobes, which account for approximately 75% of all human brain tissue, are responsible for much of the information processing that goes on between sensory input and motor output. The diverse functions of the association cortices are often referred to as cognition, which literally means the process by which we come to know the world. Selectively attending to a particular stimulus, recognizing and identifying these relevant stimulus features and planning and experiencing the response are some of the processes or abilities mediated by the human brain which are related to cognition. Compounds and compositions of the present invention may be used to enhance cognition or reduce impairment of cognitive function.

Impairment of cognitive function (“cognitive disorder”) includes memory disorders and learning disorders, which are treatable according to the present, including those disorders that result from aging, trauma, stroke, neurodegenerative disorders or anxiety disorders. Examples of neurodegenerative disorders include, but are not limited to, those associated with drug-induced states, neurotoxic agents, Alzheimer's disease, and aging. These conditions are readily recognized and diagnosed by those of ordinary skill in the art and treated by administering to the patient an effective amount of one or more compounds according to the present invention. The present invention is also useful for the treatment of attention deficit disorder (ADD), attention deficit disorder with hyperactivity (ADD-HD), autism, pervasive development disorder (PDD, including PDD-NOS), learning disabilities and disorders associated with PFC dysfunction such as and schizophrenia and bipolar disorder. etc.

The compositions of the present invention may be administered orally, parenterally, by inhalation spray, topically, rectally, nasally, buccally, vaginally or via an implanted reservoir. The term “parenteral” as used herein includes subcutaneous, intravenous, intramuscular, intra-articular, intra-synovial, intrasternal, intrathecal, intrahepatic, intralesional and intracranial injection or infusion techniques. Preferably, the compositions are administered orally, intraperitoneally, or intravenously.

Sterile injectable forms of the compositions of this invention may be aqueous or oleaginous suspension. These suspensions may be formulated according to techniques known in the art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation may also be a sterile injectable solution or suspension in a non-toxic parenterally-acceptable diluent or solvent, for example as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose, any bland fixed oil may be employed including synthetic mono- or di-glycerides. Fatty acids, such as oleic acid and its glyceride derivatives are useful in the preparation of injectables, as are natural pharmaceutically acceptable oils, such as olive oil or castor oil, especially in their polyoxyethylated versions. These oil solutions or suspensions may also contain a long-chain alcohol diluent or dispersant, such as Ph. Helv or similar alcohol.

The pharmaceutical compositions of this invention may be orally administered in any orally acceptable dosage form including, but not limited to, capsules, tablets, aqueous suspensions or solutions. In the case of tablets for oral use, carriers which are commonly used include lactose and corn starch. Lubricating agents, such as magnesium stearate, are also typically added. For oral administration in a capsule form, useful diluents include lactose and dried corn starch. When aqueous suspensions are required for oral use, the active ingredient is combined with emulsifying and suspending agents. If desired, certain sweetening, flavoring or coloring agents may also be added.

Alternatively, the pharmaceutical compositions of this invention may be administered in the form of suppositories for rectal administration. These can be prepared by mixing the agent with a suitable non-irritating excipient which is solid at room temperature but liquid at rectal temperature and therefore will melt in the rectum to release the drug. Such materials include cocoa butter, beeswax and polyethylene glycols.

The pharmaceutical compositions of this invention may also be administered topically. Suitable topical formulations are readily prepared for each of these areas or organs. Topical application for the lower intestinal tract can be effected in a rectal suppository formulation (see above) or in a suitable enema formulation. Topically-acceptable transdermal patches may also be used.

For topical applications, the pharmaceutical compositions may be formulated in a suitable ointment containing the active component suspended or dissolved in one or more carriers. Carriers for topical administration of the compounds of this invention include, but are not limited to, mineral oil, liquid petrolatum, white petrolatum, propylene glycol, polyoxyethylene, polyoxypropylene compound, emulsifying wax and water. Alternatively, the pharmaceutical compositions can be formulated in a suitable lotion or cream containing the active components suspended or dissolved in one or more pharmaceutically acceptable carriers. Suitable carriers include, but are not limited to, mineral oil, sorbitan monostearate, polysorbate 60, cetyl esters wax, cetearyl alcohol, 2-octyldodecanol, benzyl alcohol and water.

The pharmaceutical compositions of this invention may also be administered by nasal aerosol or inhalation. Such compositions are prepared according to techniques well-known in the art of pharmaceutical formulation and may be prepared as solutions in saline, employing benzyl alcohol or other suitable preservatives, absorption promoters to enhance bioavailability, fluorocarbons, and/or other conventional solubilizing or dispersing agents.

The amount of a compound used in a pharmaceutical composition of the instant invention that may be combined with the carrier materials to produce a single dosage form will vary depending upon the host treated, the particular mode of administration and may vary widely. Preferably, the compositions should be formulated to contain between about 10 milligrams to about 500 milligrams of active ingredient

It should also be understood that a specific dosage and treatment regimen for any particular patient will depend upon a variety of factors, including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, rate of excretion, drug combination, and the judgment of the treating physician and the severity of the particular disease or condition being treated.

Synthesis of Compounds According to the President Invention

The compounds according to the present invention may be synthesized by synthetic methods which are well-known in the art. The synthesis of compounds according to the present invention may proceed by following the general procedure for such compounds as presented in U.S. Pat. No. 5,223,505, relevant portions of which are incorporated by reference herein, PCT application WO 90/12790, a counterpart application to U.S. Pat. No. 5,223,505 and published Japanese patent application 2004-59465. All of the methods for synthesizing compounds according to the present invention are well-known in the art or are adapted from well-known methods in the art without undue experimentation.

In addition to the patent publication references cited above, compounds according to the present invention may be synthesized by condensing a substituted aniline compound onto a pyrimidine intermediate containing a leaving group (halogen such as Br or I) at the 6 position of a substituted 4-aminopyrimidine ring or related pyrimidine analog. The starting materials for such a synthesis may be purchased commercially or produced using methods well known in the art. Alkylation of the appropriate amino group of the resulting intermediate is effected using one or more standard alkylating agents. The resulting compound may be used directly or the counterion may be changed (ion-exchange) to reflect desired solubility/administration parameters.

The invention is described further in the following examples, which are illustrative and in way limiting.

The present invention illustrates the effects of Ih channel blockade in prefrontal cortex on 1) cognitive performance in rats engaged in a spatial working memory task, and 2) firing patterns of prefrontal cortical neurons in monkeys performing a spatial working memory task.

Example

Spatial Working Memory Performance in Rats

Rats (n=5) were tested on the spatial delayed alternation task in a T maze, a classical test of prefrontal cortical function in rodents (Larsen and Divac, Physiolog Psychol, 6:15-17, 1978). Rats were trained on the task, and then implanted with cannula aimed at the prelimbic prefrontal cortex as described in (Ramos, et al., Neuron, 40:835-845, 2003). Following recovery from surgery, rats were infused with saline (0.5 μl), the Ih channel blocker ZD7288 (0.0001 μg/0.5 μl), the cAMP analog Sp-cAMPS (0.21 nmol/0.5 μl), or a combination of ZD7288 (0.0001 μg)+Sp-cAMPS (0.21 nmol). There was at least one week washout between drug treatments, and a within subjects comparison was performed (2 way analysis of variance with repeated measures with user defined contrasts). As can be seen in FIG. 1, ZD7288 infusions into rat prefrontal cortex significantly improved working memory performance compared to saline control (F(1,4)=42.7, p=0.003). This improvement was significantly reduced by co-administration of the cAMP analog, Sp-cAMPS (F(1,4)=18.5, p=0.013), using a dose of Sp-cAMPS that has no effect on its own (p>0.3).

Similar enhancing effects have been observed previously with infusions of the α2A agonist guanfacine into prefrontal cortex of monkeys (Mao et al, Biol Psychiatry 46:1259-1265, 1999) and rats (e.g. FIG. 2). The enhancing effects of guanfacine, like those of ZD72388, can be reversed by co-infusion of Sp-cAMPS (FIG. 2). These results are consistent with the hypothesis that α2A agonists such as guanfacine strengthen prefrontal cortical cognitive function by inhibiting the production of cAMP, which in turn closes Ih channels.

Further Examples

Spatial working memory is maintained by spatially tuned, recurrent excitation within networks of prefrontal cortical (PFC) neurons, evident during delay periods in working memory tasks. Stimulation of post-synaptic α2A adrenoceptors (α2A-ARs) is critical for working memory. The following examples show that α2A-AR stimulation strengthens working memory through inhibition of cAMP, closing Hyperpolarization-activated Cyclic Nucleotide-gated (HCN) channels and strengthening the functional connectivity of PFC networks. Ultrastructurally, HCN channels and α2A-ARs were colocalized in dendritic spines in monkey PFC. In electrophysiological studies, either α2A-AR stimulation, cAMP inhibition or HCN channel blockade enhanced spatially tuned delay-related firing of PFC neurons in monkeys performing a working memory task. Conversely, delay-related network firing collapsed under conditions of excessive cAMP. In behavioral studies, HCN channel blockade or knockdown of HCN1 channels in rat PFC improved working memory performance. These data reveal a powerful mechanism for rapidly altering the strength of working memory networks in PFC.

Single Neuron Recording and Iontophoresis in Monkeys Performing a Spatial WM task

Studies were performed on 4 adult male rhesus monkeys trained on the spatial ODR task (FIG. 3). Iontophoretic electrodes, neuronal recording and drug delivery were as described in (Wang et al., 2004) and also are provided in the Supplemental Material. Guanfacine, yohimbine, ZD7288(Tocris, Ellisville, Mo.) and etazolate (Sigma, St. Louis, Mo.) were dissolved at 0.01M in triple-distilled water (adjusted with HCl to pH 3.5-4.0). Sp-cAMP and Rp-cAMP (Sigma) were dissolved at 0.01M in triple-distilled water (adjusted with NaOH to pH 9). Two-way ANOVA was used to examine the spatial tuned task-related activity with regard to: (1) different periods of the task (cue, delay, response vs. fixation) and (2) different cue locations. One-way ANOVAs were employed to assess the effect of the drug application on cells displaying delay-related activity.

Male Sprague Dawley rats (240-260 g; Taconic, Germantown, N.Y.) were trained on the delayed alternation task as described in Ramos et al., 2003. Guide cannula were implanted dorsal in prelimbic PFC (AP: +3.2 mm; ML: ±0.75 mm; DV: −4.2 mm). For infusions, needles reached to 4.5 mm DV; infused at 0.25 μL/min for 2 min. Drug or vehicle was administered in a counterbalanced order with at least 1 week between infusions. ZD7288 was dissolved in saline to a dose of 0.0001 μg/0.5 μL. Sp-cAMPS was dissolved in sterile phosphate-buffered saline at 0.21 nmol, a dose with no effect on its own, but sufficient to reverse the effects of guanfacine (Ramos et al., 2006). The experimenter was blind to treatment.

Viral Knockdown of HCN1

The coding region of HCN1 was amplified from rat brain cDNA, cloned into pSTBlue-1 (Novagen, San Diego Calif.), sequenced, and subcloned into pAAV-MCS (Stratagene, La Jolla, Calif.) to create pAAV-HCN1. A vector containing an H1 promoter for shRNA expression and a CMV promoter for lacZ expression flanked by AAV ITR sequences (pAAV-IacZ-shRNA) was created to allow viral expression of shRNAs. Two sets of shRNAs constructs (shRNA-HCN1.1 and shRNA-HCN1.2) directed against HCN1 were generated and tested in HEK293 cells by co-transfection with pAAV-HCN1. A control shRNA viral construct containing a scrambled sequence (shRNA-scrHCN1) was created with an identical nucleotide composition as the shRNA-HCN1.1 target sequence with no homology to any mammalian gene in the Genbank database. AAV2 virus was produced by transfection of HEK293 cells with pAAV-HCN1 or one of the pAAV-IacZshRNA constructs, and pDG essentially as described by (Auricchio et al., 2001). Viral titers were determined by determining viral genome copy number and by infectious titer assays in HEK293 cells.

Rats were implanted with cannula, and trained until achieving baseline performance of ˜70% correct for at least 5 consecutive days. The active or scrambled virus (5 μL) was infused into the prelimbic PFC at a rate of 0.25 μL/min WM was assessed the following day and for the subsequent 3 weeks. Rats were perfused and viral transfection was confirmed in PFC with a monoclonal antibody against B-galactosidase (Mouse anti B-gal 1:400, overnight, RT; Promega Corp., Madison, Wis.) followed by a secondary antibody (Goat anti mouse conjugated to Alexa Flour® 488, 1:400, 4 h, RT; Invitrogen/Molecular Probes, Carlsbad, Calif.). Antibodies were prepared in 0.1M PB containing 0.1% Triton X and 2% normal goat serum. All behavioral studies were performed blind to treatment.

Monkey PFC tissue (n=3) was prepared as described in the Supplemental Material. For single HCN1, HCN-pore or α2A-AR immunocytochemistry, primary antibodies were complexed with nanogold conjugates, either directly or using biotinylated bridging antibodies. F dual immunolabeling, we used combinations of enzymatic and/or gold-based immunotechniques and reversal of the immunocytochemical sequence (FIG. 8D-G). See Paspalas and Goldman-Raki (2004). Sections were processed for electron microscopy and layers I-III of area 46 were sampled for analysis under a JEM 1010 (Jeol, Tokyo, Japan) transmission electron microscope at 80 KV. Immunoreactive structures were digitally captured with a BioScan 792 (Gatan, Pleasanton, Calif.).

Results

Monkeys performed an oculomotor spatial delayed response (ODR) task, illustrated in FIG. 3A. The ODR task requires the monkey to make a memory-guided saccade to a visuo-spatial target. Stimulus position was randomized over trials to ensure use of WM. Neurons were recorded from area 46 of the dorsolateral PFC (FIG. 3B) as the monkey performed the task. FIG. 3C shows the activity of a PFC neuron with task-related firing. This neuron shows delay-related firing for the 180° location (preferred direction) but not for other directions (e.g. 0°, nonpreferred direction). In the present study, 128 neurons with delay-related mnemonic activity were isolated and subjected to iontophoretic application of pharmacological agents See Table 1, below summarizing the effects of drugs (Table 1) and their reversal (Table 2) on PFC neuronal response.

TABLE 1

Effects of manipulating α2A-AR stimulation,

cAMP signaling, or HCN channel blockade on spatial

delay-related firing of PFC neurons.

Drug Effect

Increased

Decreased

Un-changed

Guanfacine (5-15 nA)

28

(80%)

7 (20%)

Guanfacine (20-50 nA)

9

(100%)

Yohimbine

15

(100%)

Rp-cAMP

8

(67%)

4 (33%)

Sp-cAMP

9

(33%)

13

(48%)

5 (19%)

Etazolate

10

(83%)

2 (17%)

ZD7288 (5-15 nA)

19

(70%)

8 (30%)

ZD7288 (20-50 nA)

10

(100%)

TABLE 2

Summary for reversal experiments

Reversal

No Reversal

Yohimbine reversal of guanfacine

6

(100%)

Sp-cAMP reversal of guanfacine

6

(67%)

3 (33%)

Sp-cAMP reversal of Rp-cAMP

6

(100%)

ZD 7288 reversal of yohimbine

5

(71%)

2 (31%)

ZD 7288 reversal of etazolate

6

(75%)

2 (25%)

Effects of α2A-AR Stimulation on PFC Neurons with WM Activity

Intra-PFC administration of the α2A-AR agonist, guanfacine, improves WM performance (Mao et al., 1999). However, guanfacine has never been examined for its effects on PFC neuronal firing. In the present study, guanfacine was applied iontophoretically to 35 neurons with spatial delay-related activity. Low doses of guanfacine (5-15 nA) significantly enhanced delay-related activity for the preferred direction in 28 out of 35 cases, while having no effect on neuronal activity of the remaining 7 neurons. In contrast, high doses of guanfacine (20-50 nA) suppressed delay-related activity in 9 out of 9 neurons, perhaps due to stimulation of pre-synaptic α2A-ARs, reducing endogenous NE release.

FIG. 4A shows a PFC neuron with relatively weak spatial mnemonic tuning in the control condition. Following guanfacine application (10 nA), delay-related firing was substantially increased for the preferred (one-way ANOVA, P<0.0001) but not for the nonpreferred direction (P>0.05), thus enhancing spatial mnemonic tuning. In neurons with strong spatial mnemonic tuning, the effects of guanfacine (5 nA) were similar but smaller in magnitude, as shown in FIG. 4B (P<0.001). At higher doses, guanfacine (50 nA) did not have enhancing effects (FIG. 4B).

Previous studies demonstrated that iontophoretic application of yohimbine, an α2-AR antagonist, suppressed delay-related firing in PFC neurons (Li et al., 1999). Replicating these previous reports, iontophoresis of yohimbine (15 nA) suppressed delay-related activity for the preferred direction, thus eroding spatial mnemonic tuning (P<0.0001, FIG. 4C). The suppressive effects of yohimbine on delay activity occurred in 15 out of 15 cases (P<0.01 for each cell). Furthermore, co-iontophoresis of yohimbine (15 nA) reversed the enhancing effects of guanfacine on delay-related activity (P<0.01, FIG. 4D), consistent with actions at α2-ARs (population responses for guanfacine and yohimbine alone can be seen in FIGS. 4E and 4F, respectively). These results indicate that endogenous NE stimulation of α2-ARs plays an important role in strengthening delay-related firing.

Influence of cAMP on PFC Neurons Engaged in a WM Task

Behavioral studies in rats have shown that amplification of cAMP actions with Sp-cAMPS, a cAMP analog, impaired spatial WM, while inhibition of cAMP actions with Rp-cAMPS ameliorated WM deficits. Consistent with these behavioral results, we found that iontophoresis of Sp-cAMPS (10 nA) significantly decreased delay-related firing for the preferred direction (P<001, FIG. 5A), thus eroding spatial mnemonic tuning. Although the suppressive effects of Sp-cAMPS were replicated in 13 out of 27 neurons, Sp-cAMPS was found to non-specifically increase the neuronal firing in 9 of 27 neurons, and had no effect in 5 neurons. These effects may result from Sp-cAMPS blocking adenosine receptors, inducing a nonspecific increase in firing rate. Thus, we examined an alternative method for increasing cAMP signaling.

Etazolate is a PDE4 inhibitor that increases cAMP levels by inhibiting the breakdown of endogenously produced cAMP. Iontophoretic application of etazolate (25 nA) had highly consistent suppressing effects on spatial mnemonic activity in 10 of 12 PFC neurons. One example is shown in FIG. 5B, in which etazolate dramatically inhibited spatial delay-related firing (P<0.0001). The population response is shown in FIG. 5F. These effects were very rapid, occurring within minutes of application, indicating that endogenous cAMP has powerfuil suppressive effects on PFC neuronal firing.

In contrast to Sp-AMPS and etazolate, iontophoresis of the cAMP inhibitor, Rp-cAMPS (40-50 nA) specifically increased delay-related firing for the preferred direction in 8 of 12 neurons (P<0-001, FIG. 5C, population response in FIG. 5E). This enhancing effect was reversed by subsequent co-iontophoresis of Sp-cAMP (P<0.001), consistent with actions via cAMP signaling. Thus, inhibition of cAMP actions had an effect similar to that of iontophoresis of guanfacine.

Guanfacine Acts Via Inhibition of cAMP

The enhancing effects of guanfacine on WM in rats has been reversed by co-infusion of Sp-cAMPS (Ramos et al., 2006). We examined whether the enhancing effects of guanfacine on PFC neuronal firing are similarly reversed by Sp-cAMPS.

Role of HCN Channels in WM Activity

A putative downstream target of cAMP is the HCN channel. cAMP opens HCN channels, inducing an Ih current which in turn reduces membrane resistance. We examined the role of Ih in WM by iontophoresing the HCN channel blocker, ZD7288.

Low doses of ZD7288 (10 nA), like guanfacine or Rp-cAMPS, significantly increased delay-related firing for the preferred direction in 19 of 27 neurons. As with guanfacine, ZD7288 effects were most obvious in neurons with weak spatial tuning, however, small but consistent effects were also observed in well-tuned neurons with robust firing for the preferred direction under control conditions. For example, in FIG. 6A, iontophoretic application of ZD7288 (5 nA) significantly increased delay-related firing for the preferred direction (P<0.001), without altering baseline firing rates (P>0.05), or firing for the nonpreferred direction (P>0.05). ZD7288 had very robust enhancing effects on cells with weak mnemonic firing under control conditions. One example is shown in FIG. 6B, in which application of ZD7288 induced dose-dependent effects, with a low dose (5 nA) significantly increasing delay-related firing for the preferred direction (P<0.001). Subsequent application of ZD7288 at 10 nA further enhanced the delay-related activity for the preferred direction (P<0.0001). This higher dose of ZD 7288 slightly increased the background firing rate, but produced greater delay-related firing for the preferred than the nonpreferred direction (ANOVA on percentage increase, P<0.01). At the highest concentration (40 nA), ZD7288 decreased rather than increased firing (FIG. 6B). These inhibitory effects likely arose from nonspecific blockade of glutamate receptors at high doses (Chen, 2004). The population response for low dose ZD7288 application in 27 neurons is shown in FIG. 6E.

A functional link between HCN channels and α2-ARs was examined by observing whether ZD7288 could reverse the effects of α2-AR blockade (FIG. 6C). As observed above, iontophoretic application of yohimbine (15 nA) dramatically suppressed delay-related activity for the preferred direction (P<0.0001). If this collapse in firing resulted from increased cAMP production and increased numbers of open HCN channels, the yohimbine response should be reversed by co-iontophoresis of ZD7288. Indeed, subsequent co-iontophoresis of ZD7288 significantly reversed the yohimbine response, restoring spatial mnemonic activity (P<0.001). ZD7288 was able to reverse the suppressive effects of yohimbine in 7 of 9 cases (P<0.05 for each case). These data support a functional interaction between α2-ARs and HCN channels at the physiological level.

We also examined the functional interactions between HCN channels and cAMP. As shown in FIG. 6D, the reduction in delay-related firing induced by the PDE4 inhibitor, etazolate (P<0.0001) was fully reversed by co-iontophoresis of ZD7288 (P<0.001). ZD7288 reversed the suppressive effects of etazolate in 6 of 8 cases (P<0.05 for each case; FIG. 4F). Thus, blockade of HCN channels restored delay-related firing under conditions of high endogenous cAMP levels induced by either PDE4 inhibition or α2-AR blockade. These electrophysiological results demonstrate the key role of α2A/cAMP/HCN signaling in permitting spatial mnemonic Sing in PFC networks.

The cellular and network consequences of modulation of HCN channels was examined with intracellular and whole cell recordings from layer 5 pyramidal cells simultaneously with extracellular multiple unit recordings in the ferret PFC in vitro (n=55, see FIG. 6G). Under normal conditions, these slices generate Up and Down states, consisting of repeating periods of recurrent network activity (Shu et al., 2003). Bath application of ZD7288 (20-50 micromolar; n=10) resulted in a nearly 500% increase in duration of the recurrent network activity of the Up state from an average of 0.84 (±0.25) to 4.1 (±1.3) sec and an increase in Up-state action potential discharge from 1.8±1.7 Hz to 9.2±4.9 Hz (n=9). This ZD7288-induced enhancement of recurrent network activity occurred in conjunction with a progressive hyperpolarization of layer 5 pyramidal neurons (n=12), an increase in apparent input resistance (from 18.6±7.5 to 36±10.8 Mohms, n=10), an increase in the number of action potentials generated by a depolarizing current pulse (not shown), and a marked enhancement of the amplitude of synaptic barrages of the Up state from an average peak amplitude of 7.6±2.7 mV to 20.7±7-2 mV (n=21 FIG. 6G, lowest panel). These results reveal that reduction of the h-current results in significant enhancement of local recurrent network activity in cortical networks, presumably through enhanced effectiveness of dendritic synaptic potentials to initiation action potential activity (Fan et al., 2005; Magee, 1999; Nolan et al., 2004)

The next study tested the hypothesis that reducing Ih in rat prelimbic PFC (FIG. 7A) would improve performance of a spatial delayed alternation task sensitive to PFC lesions (Larsen and Divac, 1978). The prelimbic PFC displayed strong HCN1 immunoreactivity, with robust labeling of the apical dendrites in the superficial layers (FIGS. 7B, C). Ih was then reduced through either 1) blockade of HCN channels with intra-PFC ZD7288 infusions or 2) knockdown of HCN1 channel expression in PFC.

The first experiment tested whether infusion of ZD7288 into prelimbic PFC would improve performance as previously seen with guanfacine (Ramos et al., 2006) and Rp-cAMPS (Ramos et al., 2003). Pilot experiments explored a wide range of ZD7288 doses (0.00001-0.1 μg/0.5 μl). Improvement was only observed in the low dose range; thus, the 0.00011 g dose became the focus of the present study. ZD7288 was challenged with a low dose of the cAMP analog, Sp-cAMPS (0.21 nmol//0.5 μL) chosen to have no effect on its own. Results are shown in FIG. 7D. Intra-PFC infusion of ZD7288 significantly improved performance, and this effect was blocked by co-infusion of Sp-cAMPS. 2-ANOVA-R analysis showed a significant main effect of ZD7288 infusion, (F(1,4)=23.64, p=0.008); a significant main effect of Sp-cAMPS, (F(1,4)=7.37, p=0.05); and a significant interaction between ZD7288 and Sp-cAMPS (F(1,4)=30.12, p=0.005). Planned comparisons showed that infusion of ZD7288 alone significantly improved accuracy compared to saline (F(1,4)=42.67, p=0.003). Sp-cAMPS infusion had no effect on its own (F(1,4)=1.24, p=0.33), but significantly reversed the enhancing effects of ZD7288 (F(1,4)=18.46, p=0.01; ZD7288+Sp-cAMPS not different than saline: F(1,4)=0.02, p=0.88). Thus, HCN channel blockade enhanced WM at the behavioral and single-cell levels.

In the second experiment, HCN1 expression was knocked down by RNA interference in prelimbic PFC through infusion of one of two short hairpin expressing viral constructs, shRNA-HCN1.1 or shRNA-HCN1 0.2 (FIG. 7G; both were effective and so behavioral results were combined). Performance was compared to rats infused with a scrambled, inactive viral construct (shRNA-scrHCN1). Rats infused with the active constructs showed no change for the first week following infusion (mean % correct±SEM for scrambled: 72.3.4±3.6; for HCN1: 70.2±5.5%; p>0.7), but then showed significant improvements in WM performance 11-19 days after viral infusion. A 2-ANOVA-R analysis showed a significant between subjects effect of viral construct (active vs. scrambled: F(1,6)=9.57, p=0.02), a significant within subjects effect of time (pre- vs. post-infusion performance: F(1,6)=24.61, p=0.003), and a significant interaction between viral construct and time after infusion (F(1,6)=23.5, p=0.003). As shown in FIG. 5E, there was no difference between viral groups at baseline (F(1,6)=1.03, p=0.35), but a significant improvement in the active HCN1 viral group 11-19 days post-infusion (F(1,6)=34.38, p=0.0011). This is consistent with the time course of gene expression from AAV2 that we and others have observed, e.g. (Hommel et al., 2003). Post-mortem analyses confirmed viral transduction of the prelimbic PFC (FIG. 7F) and lack of expression of HCN1 in the rats receiving active construct.

Ultrastructurally, HCN1 and the pore region of HCN (corresponding to HCN1 and/or HCN2 subunits in the cortex; (Notomi and Shigemoto, 2004) were predominantly detected in dendritic spines and the shafts of pyramidal dendrites (FIGS. 8A, B). Immunoparticles in dendrites were clearly extrasynaptic (i.e. not localized at the active zone or within a 50 nm perisynaptic annulus). In favorable section planes, as in FIG. 8B, HCN channels were observed at the base of emerging spines or, typically, the neck portion. This rather unusual localization could be followed in serial sections and is unlikely to occur as a diffusion artifact, as spine heads in continuity with HCN-immunoreactive necks were often themselves immunonegative. Note that in spine heads, HCN channels appeared both at extrasynaptic membranes and perisynaptically at the edge of asymmetric, presumably excitatory synapses.

α2A-AR-immunoreactive profiles in the neuropil included the spine-laden pyramidal dendrites, besides axonal and glial localization. Distal dendrites showed plasmalemmal but also cytoplasmic labeling, indicative of a rapid turnover from the plasma membrane. Similar to HCN, α2A-AR-immunoparticles in spines marked extrasynaptic and perisynaptic membranes flanking asymmetric synapses. In addition, we observed α2A-AR labeling intracellularly and rarely within the postsynaptic specialization per se. It is worth noting that both the head and the neck portion of spines were immunoreactive for α2A-ARs, as with HCN channels (FIG. 8, compare C to B).

Dual immunolabeling confirmed the co-expression of HCN1 channels and α2A-ARs in spines and dendritic shafts at sites where emerging spines would come into focus. HCN1/α2A-AR labeling involved both the spine head and the neck portion. This pattern was reproduced with reversal of the immunocytochemical sequence, and with both peroxidase/gold (FIGS. 8D, E) and dual gold (FIGS. 8F, G) immunotechniques. Thus, in superficial layers of monkey PFC, HCN1 channels and α2A-ARs are spatially co-expressed on spine membranes.

Discussion

The current data provide the very first evidence that HCN channels have powerful effects on PFC network firing properties and cognitive performance in animals performing WM tasks. Blockade of HCN channels with ZD7288 promoted persistent network activity and enhanced the spatial mnemonic firing of PFC neurons. Similar enhancing effects were observed with stimulation of α2A-ARs or inhibition of cAMP. Conversely, increasing cAMP signaling—either directly with Sp-cAMPS, or indirectly via blockade of α2-ARs or PDE4 inhibition—dramatically suppressed delay-related firing. Blockade of HCN channels restored mnemonic activity in cells with excessive cAMP signaling induced by either blockade of α2-ARs or PDE4 inhibition, thus demonstrating a functional interaction between α2-ARs, endogenous cAMP, and HCN modulation of PFC neuronal firing. Similar effects were observed at the behavioral level, where HCN channel blockade or HCN1 channel knockdown in PFC improved spatial WM performance. Ultrastructural localization of HCN channels and α2A-ARs indicates that they are ideally situated to modulate synaptic inputs onto PFC pyramidal neurons.

Imunoelectron microscopy revealed HCN channel expression in spines of pyramidal dendrites in layers I-m of the primate PPC. Gold particles marked both extrasynaptic and perisynaptic membranes flanking asymmetric, presumed excitatory synapses. More interestingly, the spine neck was often HCN-immunoreactive. These data indicate that HCN channels are ideally suited for gating glutamatergic transmission mediated by axospinous synapses, including cortico-cortical inputs of the superficial PFC. When opened in the presence of cAMP, HCN channels would lower membrane resistance and shunt inputs to the spine. Due to the small cytosolic volume, HCN channels in the spine head—and especially those in the spine neck—are capable of “sensing” minute neurochemical changes in the milieu caused by the downstream effects of “co-localized” neurotransmitter receptors. Our ultrastructural data suggest that one such candidate receptor in PFC is the α2A-AR. It is noteworthy that the latter was found not only in the head but also in the neck of spines on both perisynaptic and extrasynaptic membranes, and dual labeling confirmed the spatial co-expression of HCN1 and α2A-ARs. α2A-ARs inhibit cAMP production via Gi signaling and thus could have a powerfuil influence on the open-state of nearby HCN channels. Inadequate stimulation of α2A-ARs would result in elevated cytosolic cAMP levels, opening Ih channels, and selectively disconnecting axospinous inputs to cortical pyramids. Therefore, cytosol compartmentalization in the spine and the co-expression of HCN channels with α2A-ARs on spine membranes may provide a cellular basis for altering the strength of excitatory transmission at individual axospinous synapses, thus modulating circuit connectivity in PFC.

The functional implications suggested by immunoelectron microscopy were supported by our physiological data. Delay-related firing arises from reverberating, excitatory circuits in PFC, and depends on the functional connectivity of neurons with shared spatial tuning. The current study found that agents, which 1) stimulate α2A-ARs, 2) inhibit cAMP signaling or 3) block HCN channels, all increase delay-related firing for the preferred direction, consistent with increased functional connectivity under conditions where HCN channels are closed. Conversely, delay-related firing collapsed in the presence of agents that blocked α2A-ARs or increased cAMP signaling, consistent with reduced functional connectivity when cAMP opens HCN channels. These modulatory effects were very powerful: extremely low doses selectively altered delay-related firing for the preferred direction, while slightly higher doses had more generalized effects on firing, consistent with perturbations in network firing. Parallel results were observed at the behavioral level, where PFC infusions of guanfacine (Ramos et al., 2006) or ZD7288, or knockdown of HCN1 expression in PFC, all improved WM performance, while agents that blocked α2A-ARs (Li and Mei, 1994) or accentuated cAMP signaling (Taylor et al., 1999) impaired WM. It is remarkable to have such confluence between behavioral and electrophysiological findings.

ZD7288 is currently the most selective HCN antagonist available, and as such, has become the standard pharmacological method for assessing Ih mechanisms, e.g. (Fan et al., 2005). However, it has recently been noted that at higher doses ZD7288 becomes nonselective, producing ALA and NMDA glutamate receptor blockade (Chen, 2004). The inhibition of cell activity at higher ZD7288 concentrations (25-40 nA) in the current study could be consistent with reduced glutamate receptor excitation. A similar profile was observed in our behavioral data, where pilot studies showed that higher doses of ZD7288 were ineffective, whereas a very low dose consistently improved WM performance.

Physiological interactions between α2A-ARs and HCN channels were observed, consistent with their co-expression in spines: The collapse in memory fields induced by the α2A-AR antagonist, yohimbine, was reversed by the HCN channel blocker, ZD7288. HCN channel blockade similarly reversed the suppressive effects of the PDE4 inhibitor, etazolate. Thus, iontophoresis of yohimbine, Sp-cAMPS or etazolate, all induced an immediate collapse in memory fields, likely due to a reduction in recurrent excitatory drive in the PFC network. The blockade of HCN channels with ZD7288 restored a normal firing pattern. It should be noted that these agents are not likely competing at the same individual channel; rather, it is likely that ZD7288 and Sp-cAMPS (or cAMP per se) each alter the open state of a subset of HCN channels, and we record the integration of this population response. It is striking that very low doses can have such rapid and robust effects on cell firing, revealing a powerful mechanism for the dynamic regulation of PFC microcircuits.

Comparison to HCN Actions on Long-Term Memory Functions of the Hippocampus and Cerebellum

Recent studies of HCN1 knockout mice have revealed distinct roles of Ih in cerebellum and hippocampus, and it is instructive to compare the current results with these important findings. In cerebellar Purkinje cells, HCN1 channels mediate an inward current that stabilizes the integrative properties of the cell and ensures that their input-output function is independent of previous activity (Nolan et al., 2003). HCN1 currents allow Purkinje cells to integrate information very quickly, consistent with the rapid time scale of cerebellar mechanisms (ibid). However, the influence of HCN channels in cerebellum occurs only when Purkinje cells are hyperpolarized. In contrast, pyramidal cells in hippocampus and PFC have a lower resting membrane potential, and thus HCN channels may play a role under resting conditions in these cells (Nolan et al., 2004). Pyramidal cells in PFC and hippocampus share other properties: HCN1 channels are localized on their distal dendrites, and, when opened, appear to shunt synaptic inputs onto those distal locations (Magee, 1999; Nolan et al., 2004). However, there are important differences between CA1 and PFC neurons as well. In hippocampus, high levels of cAMP are essential for long-term potentiation and memory consolidation, leading to long-lasting changes in synaptic architecture (cAMP also plays a beneficial role when hippocampus interacts with PFC under conditions when delay lengths are very long, see (Runyan and Dash, 2005). In contrast, the WM operations of the PFC depend on the transient activation of microcircuits that are disrupted by high levels of cAMP. Thus, cAMP/HCN signaling may play an especially important role in PFC.

Model of α2A-cAMP HCN Modulation of PFC Networks

FIG. 9 illustrates a model whereby HCN channels on spines of PFC distal pyramidal dendrites modulate the efficacy of excitatory inputs. Pyramidal cells form reverberating circuits through mutual, axospinous excitatory connections. HCN channels are localized on the spine neck or near excitatory synapses on spine head membranes. When HCN channels are open in the presence of cAMP, they pass Ih which lowers membrane resistance and effectively shunts synaptic input, reducing the functional connectivity of the network (FIG. 9A). NE regulates this process. We have shown α2A-ARs and HCN1 channels on the same spine membranes. Stimulation of α2A-ARs, e.g. with guanfacine, inhibits the production of cAMP, closing HCN channels and increasing the efficacy of synaptic inputs, thus strengthening the functional connectivity of PFC microcircuits (FIG. 9B). Thus, α2A/cAMP/HCN signaling provides a mechanism for dynamically regulating the strength of PFC networks.

The examples further indicate that Gs-coupled receptors may temporarily suppress neurotransmission by activating cAMP production, opening HCN channels and shunting synaptic inputs. For example, dopamine D1 receptors (D1Rs) are coupled to Gs and are also concentrated on spines in superficial PFC (Smiley et al., 1994). We have recently observed that moderate levels of D1R stimulation suppress firing for nonpreferred spatial directions via a cAMP-mediated mechanism (Vijayraghavan et al., 2007). It is not known if this suppression involves opening of HCN channels. If so, it is possible that α2A-ARs amplify inputs for preferred directions by selectively closing HCN channels on spines receiving inputs from neurons with shared spatial preferences, while D1Rs may shunt inputs from neurons tuned to nonpreferred directions. Thus, the open state of HCN channels may determine which pattern of microcircuits are functionally connected at any one time to appropriately regulate behavior and thought based on immediate cognitive demands.

Relevance of α2A-AR-cAMP-HCN Signaling to Arousal State and Stress

αa2A/cAMP/HCN signaling in superficial cortical layers likely regulates the strength of PFC function based on the animal's state of arousal. Low levels of NE cell firing during drowsy conditions (Aston-Jones et al., 1999) may lead to insufficient NE stimulation of α2A-ARs, inadequate inhibition of cAMP, and impaired WM. Conversely, exposure to uncontrollable stress impairs WM via excessive catecholamine release (Arnsten, 2000). Similar effects are observed at the cellular level, where memory fields collapse under neurochemical conditions induced by stress: e.g. etazolate, Sp-cAMPS, (FIG. 3) or high levels of D1R stimulation activating cAMP (Vijayraghavan et al., 2007). Thus, under conditions of uncontrollable stress, cortico-cortical connections of superficial PFC would be functionally disconnected, rendering the PFC “de-corticate”. This process may be exacerbated in patients with alterations in genes that regulate cAMP signaling, e.g. COMT (Egan et al., 2001) or DISC1 (Millar et al., 2005), increasing vulnerability to PFC dysfunction in illnesses such as schizophrenia that are worsened by stress exposure. Intriguingly, DISC1 protein has been localized to dendritic spines in human PFC, (Kirkpatrick et al., 2006), suggesting that it may normally regulate Ih, but may inadequately suppress cAMP levels in spines of patients with schizophrenia Guanfacine has recently been shown to strengthen PFC cognitive function in patients with schizotypal disorder (McClure et al., 2006), as well as those with ADHD (Scahil et al., 2001; Taylor and Russo, 2001). The present data suggest that some of these enhancing effects may result from reduced cAMP production and the closure of HCN channels. These results represent the first time that we have understood the mechanism of action of a psychotropic medication at the level of an ion channel, and illustrate the powerful influence of arousal pathways on cortical microcircuitry.

The previously described examples demonstrate that HCN1 or HCN1/HCN2 heteromers in spines of pyramidal dendrites in the superficial layers of primate PFC are spatially co-expressed with the α2A-AR, thus providing a potent substratum for functional interaction. Electrophysiological and cognitive experiments support a model where α2A-AR agonists such as guanfacine improve PFC cognitive function by inhibiting the production of cAMP, closing HCN channels, and strengthening the PFC networks that underlie delay-related cell firing in monkeys performing a spatial WM task.

It is to be understood by those skilled in the art that the foregoing description and examples are illustrative of practicing the present invention, but are in no way limiting. Variations of the detail presented herein may be made without departing from the spirit and scope of the present invention as defined by the following claims.