The artifacts belonging to the Mannaean culture that are preserved in major museums worldwide display symbolic images indicating hidden values and meanings derived from their common beliefs and traditions. An analytical study of the reminiscence of the symbols used in these objects reveal the influence of religion and ritual traditions in Mannaean societies and their deep relations with the Neo-Assyrian culture. In this research, by examining and analyzing the most prominent symbolic images such as the sacred tree, winged gods, mythological creatures, Ishtar goddess on golden plaque, earthenware, ivory plaques and other objects found in Ziwiye, Hasanlu and Qalaichitepes as well as ivory objects with pictures of battle scenes, chariot riding and formal ceremonies found at Mannaean sites, we try to take a closer look at the impact of the Neo- Assyrian art on the Mannaean art and culture.