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アプリケーション

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション

Abreviews

特記事項

IHC-Fr

Use at an assay dependent concentration.

ターゲット情報

機能

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.

配列類似性

Belongs to the bZIP family. Fos subfamily.Contains 1 bZIP domain.

翻訳後修飾

Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation.Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.

We do not carry any anti-c-Fos antibodies that are known to work in rabbit. However, we have a few polyclonal antibodies that aren't raised in rabbit which may work due to high sequence homology with rabbit. Read More

DISCOUNT CODE: XXXXXXX
Expiration date: [15/03/2012]
I am very pleased to hear you would like to accept our offer and test ab6167 in FrFl. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please s...

Thank you for your interest.
I can certainly offer a discount code for testing ab6167 for FrFl, this antibody has only been tested in IHC-Fr (mouse and human). Could you please pass the customer's name, e-mail address and institute?
I look forw...

Thank you for your enquiry. All the information we have available concerning this antibody is that listed on our datasheet. We are not aware of there being publications featuring this particular antibody. All we have are general background references. ...

Thank you for your enquiry. Although the caps are different, the antibody is from the same batch of animal. We are not sure why there is difference to the second vial.
We will send you a replacement vial in the next order.

There is nothing special about this antibody regarding its use in
immunohistochemistry. The antibody can be used with a routine 4%
formaldehyde fixation (we usually add 15% saturated picric acid) on
cryostat sections. Thus, use a standard IHC pro...

We have only recently
released this antibody into the market, and so have not yet had any feedback from customers. However, this antibody has been used extensively in research (the source laboratory is not in
the US), often in double label experiment...