Retroviral Vectors for RNAi—shRNA Gene Knockdown Using Retrovirus

Retroviral vectors that express shRNA for gene knockdown are available with a constitutive U6 promoter with or without a fluorescent protein reporter. For inducible expression of shRNA see our tetracycline inducible shRNA product page.

RNAi-Ready pSIREN-RetroQ Vectors

RNAi-Ready pSIREN-RetroQ Vectors are retroviral expression vectors based on our Q-Series of retroviral vectors, which are designed to constitutively express an shRNA from the human U6 promoter. These systems are ideal for shRNA delivery into hard-to-transfect cells. Package your RetroQ vector using the Retro-X Universal Packaging System to generate the optimum retrovirus for your cell type.

These vectors are available with Living Colors Fluorescent Protein reporters (Cat. Nos. 632455 & 632487).

Efficient Delivery of shRNA Using Retroviral Transduction

Retroviral transduction enables you to deliver shRNA into 100% of of your target cells, which means that you can achieve knockdown of gene expression in the entire cell population rather than a just portion of it. Even the most efficient plasmid transfections leave a percentage of cells untransfected and thus with no gene knockdown. shRNA delivered using virus therefore increases the dynamic range of knockdown experiments.

Applications

Gene knockdown in hard-to-transfect cells

Delivery to 100% of your target cells

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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