Cancer researchers dream of the day they can force tumor cells to morph back to the normal cells they once were. Now, researchers on Mayo Clinic’s Florida campus have discovered a way to potentially reprogram cancer cells back to normalcy.
Panos Anastasiadis, Ph.D., chair of the Department of Cancer Biology on Mayo Clinic’s Florida campus comments on the findings which are published in Nature Cell Biology.
Learn more on this here: http://goo.gl/pvV5xa

E.S.P. (Extra Sexual Persuasion)

E.S.P. (Extra Sexual Persuasion) is a Millie Jackson album released in 1983. In addition to her signature soul music songs, it also includes somewhat more Hi-NRG and Funk dance song production popular at the time such as "This Girl Could Be Dangerous", "Sexercise" and the title track.

Critical reception

In a contemporary review for The Village Voice, music critic Robert Christgau gave the album a "B-" and wrote that, despite her mannerisms and persuasive parodies of sexercise, Jackson lacks the redeeming slow songs of her past work, and both "Slow Tongue" and the title track sound contrived.

E.S.P. (Miles Davis album)

Recorded in January 1965, E.S.P. is the first album by what is often referred to as Miles Davis's second great quintet. The quintet comprising Davis, Wayne Shorter, Herbie Hancock, Ron Carter and Tony Williams would be the most long-lived of all Davis's groups, and this was their first studio recording together.

Composition

Unlike the majority of previous Davis albums, E.S.P. consisted entirely of new compositions written by members of the group. Despite the profusion of new material, only two of the tunes, "Agitation" and "R.J." are known to have appeared in the group's live performances, the latter only appearing in one extant recording. "Agitation", by contrast, was still being performed as late as the fall of 1969.

"Little One" might be best known for being revisited on Hancock's landmark album, Maiden Voyage, recorded a few weeks later. This version is somewhat more embryonic; Carter's bass is halting, and Davis and Shorter state the theme with winding, interlocking contrapuntal lines that evoke Davis and Coltrane's version of "Round Midnight". Hancock's solo on Carter's composition, "Eighty-One", also presages his work on that LP - particularly its title track. This is reflected in the liner notes of the 1999 reissue.

Mayo Clinic

Mayo Clinic is a nonprofit medical practice and medical research group based in Rochester, Minnesota. It is the first and largest integrated nonprofit medical group practice in the world, employing more than 3,800 physicians and scientists and 50,900 allied health staff. The practice specializes in treating difficult cases through tertiary care. It spends over $500 million a year on research.

Dr. William Worrall Mayo settled his family in Rochester in 1864 and opened a medical practice that evolved under his sons into Mayo Clinic. Mayo Clinic is widely regarded as one of the world's greatest hospitals and ranked No. 1 on the 2014–2015 U.S. News & World Report List of "Best Hospitals", maintaining a position near the top for more than 20 years. It has been on the list of America's "100 Best Companies to Work For" published by Fortune magazine for eight consecutive years. It continued to achieve this ranking through 2015.

In addition to their flagship hospital in Rochester, the Mayo Clinic has major campuses in Arizona and Florida. The Mayo Clinic Health System also operates affiliated facilities throughout Minnesota, Wisconsin, and Iowa.

The Rain

The Rain were the Manchester band that eventually evolved into Oasis. The band formed in Manchester, England in 1991, taking their name from The Beatles' B-side, "Rain". Founding members were Paul "Bonehead" Arthurs (guitar), Paul "Guigsy" McGuigan (bass), Chris Hutton (vocals). Drummer Tony McCarroll joined shortly after the band formed to replace their drum machine and Hutton was replaced as vocalist by Liam Gallagher, who became the band's songwriter, in partnership with Arthurs. In this partnership, they wrote several songs including "Take Me" and "Life in Vain". The band rehearsed only one day a week and did not perform at shows often.

Shortly after Liam joined, the band was renamed Oasis, at his suggestion. Various explanations of the origins of the name have been offered, however, it came about when Liam’s older brother Noel roadied for the Inspiral Carpets at a venue in Swindon called the Oasis Leisure Centre. Liam reportedly liked the name's "resonance of imagery."

One day in August 1991, Noel, having recently returned from the Inspiral Carpets' tour of the U.S., went to watch his brother's band perform at the Manchester Boardwalk, supporting a band called Sweet Jesus. Noel offered to join, reportedly on condition that he would be the lead guitarist and they would perform only his songs. Noel made an instant impact on the band, he began to introduce new sounds and ways of creating music. After a few small warm up gigs in towns such as Middlesbrough, the band decided to produce Definitely Maybe, under the name of Oasis.

Cancer researchers dream of the day they can force tumor cells to morph back to the normal cells they once were. Now, researchers on Mayo Clinic’s Florida campus have discovered a way to potentially reprogram cancer cells back to normalcy.
Panos Anastasiadis, Ph.D., chair of the Department of Cancer Biology on Mayo Clinic’s Florida campus comments on the findings which are published in Nature Cell Biology.
Learn more on this here: http://goo.gl/pvV5xa

Learn more at http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Stem-Cell-Research/Induced-Pluripotent-Stem-Cells/Sendai-Virus-Reprogramming.html
Dr. Uma Lakshmipathy presents work on the creation of integration-free induced pluripotent stem cells at high efficiency with Sendai Virus using the CytoTune™ -iPS Sendai Reprogramming Kit.
[audio transcript] So to start with the most common method for generating iPSC is transduction of the four factors and shown here is the Yamanaka factors and after a black box in which takes anywhere between three to four weeks you end up with iPSC colonies. So the biggest bottleneck right now; one is the efficiency of iPSC formation depending on what kinds of cells you start with, the efficiency is really low.
And the second thing, the second bottleneck is how do you detect these emerging iPSC colonies depending on the expertise of the users and if you look for pluripotent stem cells, people can either pick it really easily or there is always like an issue on what clones you place your bet on.
So when it comes to efficient methods, there are several methods starting from viral non-integrating and more small molecule methods such as mRNA, microRNA and small molecules. But one of the methods we really worked on is a non-intergrating viral method based on Sendai virus. The reason why this method is superior to the other current methods is its efficiency; it's based on Sendai virus which is a RNA virus.
So again coming back to the different methods that are out there, if you were to... this is a graph I took from a published paper... if you look at the efficiency versus the safety obviously methods suggests small molecule: microRNA, RNA and protein they don't leave a footprint, they are extremely safe to use in a clinical setting; however the efficiency of generating iPSC right now is pretty low at this point of time.
The highest efficiency so far has been obtained with viral methods such as lenti and retro; more recently the CytoTune which I will show you some data. Actually excels the efficiency that you can actually get with this traditional viral system and at the same time it's relatively much safer because it's a RNA virus and it's non-integrating. Therefore it will not leave a footprint in the gene of the cells or iPSCs that are created.
So this is again a brief introduction, this was a system that was developed by a company in Japan called DNAVEC. The original paper was published; there have been several papers since then starting from generation of iPSCs not only with fiberblast but also blood cells.
So I won't go into more details there, but what I would like to show you is that using the CytoTune System that we actually sell as a product, the process of generating iPSC is extremely streamline. The four factors come in four tubes, which can me transduced overnight onto maps. Most of our protocols right now are for fiberblast, but we are developing other cell types, mainly blood lineages. After, it's a one-time contact; you don't have to do repeated transductions so in that way it is really workflow friendly.
After transduction you have to give it around three or four weeks. At the beginning of three weeks, is when you start colony formation. At the end of four weeks you have sufficient colonies to pick up and choose. There actually more colonies than you really want be cause there are way to many colonies there.
So what we've done; here this method actually shows you that its integration free. Using PCR, we were able to show that there was no viral genome left in the clones that were established. This is 10 independent clones that were generated. You can also use an antibody, although I would say the antibodies not really that great because you can see the haze in the negative cell type, and you actually can tell when it's negative only when you have a true positive control because the positive staining is so much more robust. These cells are clearly important both in their marker expression, and differentiate into different lineages, when randomly differentiated by embryonic body formation.
These clones were all generated on feeder dependent systems [...], but since then we have also been able to generate iPSC clones both under feeder free conditions using StemPro SFM media as well as Xeno-free conditions, which is basically KRS-XF media in the presence of growth factor cocktails on human feeders.
Learn more at http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Stem-Cell-Research/Induced-Pluripotent-Stem-Cells/Sendai-Virus-Reprogramming.html

After delivering 10 tons of emergency supplies to aid families impacted by Japan's devastating quake and tsunami, Operation Blessing is now helping survivors see clearly through the distribution of over 1,000 pairs of eyeglasses.
-~-~~-~~~-~~-~-
Please watch: "Health Workers in Haiti: A Day in the Life"
https://www.youtube.com/watch?v=ZR2l338Kd7o
-~-~~-~~~-~~-~-

SENDAI :: EZRA BROWN hosted by Barry Likumahuwa

Gospel, blues, jazz, r&b, soul, hip-hop—language of the human experience, EzraBrown’s music speaks this language. His horn serves as his translator, becoming a voice that speaks in tongues transcending any language barrier. Ezra’s sound — a culmination of southern comfort and urban grit, gives birth to a style that is new, yet familiar. This sound speaks from the soul. A sound Ezra calls Soul Muzik. When he picks up his horn, sound is transformed into a language that enters the soul producing an experience that is pure music. An experience that goes beyond sound becoming something that is felt. Music that translates the human experience for all to understand.
Ezra Brown uses his musical style to transform a mere story to a lyrical moment. This style makes him a much sought after force am...

Cancer researchers dream of the day they can force tumor cells to morph back to the normal cells they once were. Now, researchers on Mayo Clinic’s Florida campus have discovered a way to potentially reprogram cancer cells back to normalcy.
Panos Anastasiadis, Ph.D., chair of the Department of Cancer Biology on Mayo Clinic’s Florida campus comments on the findings which are published in Nature Cell Biology.
Learn more on this here: http://goo.gl/pvV5xa

Learn more at http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Stem-Cell-Research/Induced-Pluripotent-Stem-Cells/Sendai-Virus-Reprogramming.html
Dr. Uma Lakshmipathy presents work on the creation of integration-free induced pluripotent stem cells at high efficiency with Sendai Virus using the CytoTune™ -iPS Sendai Reprogramming Kit.
[audio transcript] So to start with the most common method for generating iPSC is transduction of the four factors and shown here is the Yamanaka factors and after a black box in which takes anywhere between three to four weeks you end up with iPSC colonies. So the biggest bottleneck right now; one is the efficiency of iPSC formation depending on what kinds of cells you start with, the efficiency is really low.
And the second th...

After delivering 10 tons of emergency supplies to aid families impacted by Japan's devastating quake and tsunami, Operation Blessing is now helping survivors see clearly through the distribution of over 1,000 pairs of eyeglasses.
-~-~~-~~~-~~-~-
Please watch: "Health Workers in Haiti: A Day in the Life"
https://www.youtube.com/watch?v=ZR2l338Kd7o
-~-~~-~~~-~~-~-

Cancer researchers dream of the day they can force tumor cells to morph back to the normal cells they once were. Now, researchers on Mayo Clinic’s Florida campu...

Cancer researchers dream of the day they can force tumor cells to morph back to the normal cells they once were. Now, researchers on Mayo Clinic’s Florida campus have discovered a way to potentially reprogram cancer cells back to normalcy.
Panos Anastasiadis, Ph.D., chair of the Department of Cancer Biology on Mayo Clinic’s Florida campus comments on the findings which are published in Nature Cell Biology.
Learn more on this here: http://goo.gl/pvV5xa

Cancer researchers dream of the day they can force tumor cells to morph back to the normal cells they once were. Now, researchers on Mayo Clinic’s Florida campus have discovered a way to potentially reprogram cancer cells back to normalcy.
Panos Anastasiadis, Ph.D., chair of the Department of Cancer Biology on Mayo Clinic’s Florida campus comments on the findings which are published in Nature Cell Biology.
Learn more on this here: http://goo.gl/pvV5xa

Learn more at http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Stem-Cell-Research/Induced-Pluripotent-Stem-Cells/Sendai-Virus-Reprog...

Learn more at http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Stem-Cell-Research/Induced-Pluripotent-Stem-Cells/Sendai-Virus-Reprogramming.html
Dr. Uma Lakshmipathy presents work on the creation of integration-free induced pluripotent stem cells at high efficiency with Sendai Virus using the CytoTune™ -iPS Sendai Reprogramming Kit.
[audio transcript] So to start with the most common method for generating iPSC is transduction of the four factors and shown here is the Yamanaka factors and after a black box in which takes anywhere between three to four weeks you end up with iPSC colonies. So the biggest bottleneck right now; one is the efficiency of iPSC formation depending on what kinds of cells you start with, the efficiency is really low.
And the second thing, the second bottleneck is how do you detect these emerging iPSC colonies depending on the expertise of the users and if you look for pluripotent stem cells, people can either pick it really easily or there is always like an issue on what clones you place your bet on.
So when it comes to efficient methods, there are several methods starting from viral non-integrating and more small molecule methods such as mRNA, microRNA and small molecules. But one of the methods we really worked on is a non-intergrating viral method based on Sendai virus. The reason why this method is superior to the other current methods is its efficiency; it's based on Sendai virus which is a RNA virus.
So again coming back to the different methods that are out there, if you were to... this is a graph I took from a published paper... if you look at the efficiency versus the safety obviously methods suggests small molecule: microRNA, RNA and protein they don't leave a footprint, they are extremely safe to use in a clinical setting; however the efficiency of generating iPSC right now is pretty low at this point of time.
The highest efficiency so far has been obtained with viral methods such as lenti and retro; more recently the CytoTune which I will show you some data. Actually excels the efficiency that you can actually get with this traditional viral system and at the same time it's relatively much safer because it's a RNA virus and it's non-integrating. Therefore it will not leave a footprint in the gene of the cells or iPSCs that are created.
So this is again a brief introduction, this was a system that was developed by a company in Japan called DNAVEC. The original paper was published; there have been several papers since then starting from generation of iPSCs not only with fiberblast but also blood cells.
So I won't go into more details there, but what I would like to show you is that using the CytoTune System that we actually sell as a product, the process of generating iPSC is extremely streamline. The four factors come in four tubes, which can me transduced overnight onto maps. Most of our protocols right now are for fiberblast, but we are developing other cell types, mainly blood lineages. After, it's a one-time contact; you don't have to do repeated transductions so in that way it is really workflow friendly.
After transduction you have to give it around three or four weeks. At the beginning of three weeks, is when you start colony formation. At the end of four weeks you have sufficient colonies to pick up and choose. There actually more colonies than you really want be cause there are way to many colonies there.
So what we've done; here this method actually shows you that its integration free. Using PCR, we were able to show that there was no viral genome left in the clones that were established. This is 10 independent clones that were generated. You can also use an antibody, although I would say the antibodies not really that great because you can see the haze in the negative cell type, and you actually can tell when it's negative only when you have a true positive control because the positive staining is so much more robust. These cells are clearly important both in their marker expression, and differentiate into different lineages, when randomly differentiated by embryonic body formation.
These clones were all generated on feeder dependent systems [...], but since then we have also been able to generate iPSC clones both under feeder free conditions using StemPro SFM media as well as Xeno-free conditions, which is basically KRS-XF media in the presence of growth factor cocktails on human feeders.
Learn more at http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Stem-Cell-Research/Induced-Pluripotent-Stem-Cells/Sendai-Virus-Reprogramming.html

Learn more at http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Stem-Cell-Research/Induced-Pluripotent-Stem-Cells/Sendai-Virus-Reprogramming.html
Dr. Uma Lakshmipathy presents work on the creation of integration-free induced pluripotent stem cells at high efficiency with Sendai Virus using the CytoTune™ -iPS Sendai Reprogramming Kit.
[audio transcript] So to start with the most common method for generating iPSC is transduction of the four factors and shown here is the Yamanaka factors and after a black box in which takes anywhere between three to four weeks you end up with iPSC colonies. So the biggest bottleneck right now; one is the efficiency of iPSC formation depending on what kinds of cells you start with, the efficiency is really low.
And the second thing, the second bottleneck is how do you detect these emerging iPSC colonies depending on the expertise of the users and if you look for pluripotent stem cells, people can either pick it really easily or there is always like an issue on what clones you place your bet on.
So when it comes to efficient methods, there are several methods starting from viral non-integrating and more small molecule methods such as mRNA, microRNA and small molecules. But one of the methods we really worked on is a non-intergrating viral method based on Sendai virus. The reason why this method is superior to the other current methods is its efficiency; it's based on Sendai virus which is a RNA virus.
So again coming back to the different methods that are out there, if you were to... this is a graph I took from a published paper... if you look at the efficiency versus the safety obviously methods suggests small molecule: microRNA, RNA and protein they don't leave a footprint, they are extremely safe to use in a clinical setting; however the efficiency of generating iPSC right now is pretty low at this point of time.
The highest efficiency so far has been obtained with viral methods such as lenti and retro; more recently the CytoTune which I will show you some data. Actually excels the efficiency that you can actually get with this traditional viral system and at the same time it's relatively much safer because it's a RNA virus and it's non-integrating. Therefore it will not leave a footprint in the gene of the cells or iPSCs that are created.
So this is again a brief introduction, this was a system that was developed by a company in Japan called DNAVEC. The original paper was published; there have been several papers since then starting from generation of iPSCs not only with fiberblast but also blood cells.
So I won't go into more details there, but what I would like to show you is that using the CytoTune System that we actually sell as a product, the process of generating iPSC is extremely streamline. The four factors come in four tubes, which can me transduced overnight onto maps. Most of our protocols right now are for fiberblast, but we are developing other cell types, mainly blood lineages. After, it's a one-time contact; you don't have to do repeated transductions so in that way it is really workflow friendly.
After transduction you have to give it around three or four weeks. At the beginning of three weeks, is when you start colony formation. At the end of four weeks you have sufficient colonies to pick up and choose. There actually more colonies than you really want be cause there are way to many colonies there.
So what we've done; here this method actually shows you that its integration free. Using PCR, we were able to show that there was no viral genome left in the clones that were established. This is 10 independent clones that were generated. You can also use an antibody, although I would say the antibodies not really that great because you can see the haze in the negative cell type, and you actually can tell when it's negative only when you have a true positive control because the positive staining is so much more robust. These cells are clearly important both in their marker expression, and differentiate into different lineages, when randomly differentiated by embryonic body formation.
These clones were all generated on feeder dependent systems [...], but since then we have also been able to generate iPSC clones both under feeder free conditions using StemPro SFM media as well as Xeno-free conditions, which is basically KRS-XF media in the presence of growth factor cocktails on human feeders.
Learn more at http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Stem-Cell-Research/Induced-Pluripotent-Stem-Cells/Sendai-Virus-Reprogramming.html

After delivering 10 tons of emergency supplies to aid families impacted by Japan's devastating quake and tsunami, Operation Blessing is now helping survivors see clearly through the distribution of over 1,000 pairs of eyeglasses.
-~-~~-~~~-~~-~-
Please watch: "Health Workers in Haiti: A Day in the Life"
https://www.youtube.com/watch?v=ZR2l338Kd7o
-~-~~-~~~-~~-~-

After delivering 10 tons of emergency supplies to aid families impacted by Japan's devastating quake and tsunami, Operation Blessing is now helping survivors see clearly through the distribution of over 1,000 pairs of eyeglasses.
-~-~~-~~~-~~-~-
Please watch: "Health Workers in Haiti: A Day in the Life"
https://www.youtube.com/watch?v=ZR2l338Kd7o
-~-~~-~~~-~~-~-

Cancer researchers dream of the day they can force tumor cells to morph back to the normal cells they once were. Now, researchers on Mayo Clinic’s Florida campus have discovered a way to potentially reprogram cancer cells back to normalcy.
Panos Anastasiadis, Ph.D., chair of the Department of Cancer Biology on Mayo Clinic’s Florida campus comments on the findings which are published in Nature Cell Biology.
Learn more on this here: http://goo.gl/pvV5xa

Learn more at http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Stem-Cell-Research/Induced-Pluripotent-Stem-Cells/Sendai-Virus-Reprogramming.html
Dr. Uma Lakshmipathy presents work on the creation of integration-free induced pluripotent stem cells at high efficiency with Sendai Virus using the CytoTune™ -iPS Sendai Reprogramming Kit.
[audio transcript] So to start with the most common method for generating iPSC is transduction of the four factors and shown here is the Yamanaka factors and after a black box in which takes anywhere between three to four weeks you end up with iPSC colonies. So the biggest bottleneck right now; one is the efficiency of iPSC formation depending on what kinds of cells you start with, the efficiency is really low.
And the second thing, the second bottleneck is how do you detect these emerging iPSC colonies depending on the expertise of the users and if you look for pluripotent stem cells, people can either pick it really easily or there is always like an issue on what clones you place your bet on.
So when it comes to efficient methods, there are several methods starting from viral non-integrating and more small molecule methods such as mRNA, microRNA and small molecules. But one of the methods we really worked on is a non-intergrating viral method based on Sendai virus. The reason why this method is superior to the other current methods is its efficiency; it's based on Sendai virus which is a RNA virus.
So again coming back to the different methods that are out there, if you were to... this is a graph I took from a published paper... if you look at the efficiency versus the safety obviously methods suggests small molecule: microRNA, RNA and protein they don't leave a footprint, they are extremely safe to use in a clinical setting; however the efficiency of generating iPSC right now is pretty low at this point of time.
The highest efficiency so far has been obtained with viral methods such as lenti and retro; more recently the CytoTune which I will show you some data. Actually excels the efficiency that you can actually get with this traditional viral system and at the same time it's relatively much safer because it's a RNA virus and it's non-integrating. Therefore it will not leave a footprint in the gene of the cells or iPSCs that are created.
So this is again a brief introduction, this was a system that was developed by a company in Japan called DNAVEC. The original paper was published; there have been several papers since then starting from generation of iPSCs not only with fiberblast but also blood cells.
So I won't go into more details there, but what I would like to show you is that using the CytoTune System that we actually sell as a product, the process of generating iPSC is extremely streamline. The four factors come in four tubes, which can me transduced overnight onto maps. Most of our protocols right now are for fiberblast, but we are developing other cell types, mainly blood lineages. After, it's a one-time contact; you don't have to do repeated transductions so in that way it is really workflow friendly.
After transduction you have to give it around three or four weeks. At the beginning of three weeks, is when you start colony formation. At the end of four weeks you have sufficient colonies to pick up and choose. There actually more colonies than you really want be cause there are way to many colonies there.
So what we've done; here this method actually shows you that its integration free. Using PCR, we were able to show that there was no viral genome left in the clones that were established. This is 10 independent clones that were generated. You can also use an antibody, although I would say the antibodies not really that great because you can see the haze in the negative cell type, and you actually can tell when it's negative only when you have a true positive control because the positive staining is so much more robust. These cells are clearly important both in their marker expression, and differentiate into different lineages, when randomly differentiated by embryonic body formation.
These clones were all generated on feeder dependent systems [...], but since then we have also been able to generate iPSC clones both under feeder free conditions using StemPro SFM media as well as Xeno-free conditions, which is basically KRS-XF media in the presence of growth factor cocktails on human feeders.
Learn more at http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Stem-Cell-Research/Induced-Pluripotent-Stem-Cells/Sendai-Virus-Reprogramming.html

After delivering 10 tons of emergency supplies to aid families impacted by Japan's devastating quake and tsunami, Operation Blessing is now helping survivors see clearly through the distribution of over 1,000 pairs of eyeglasses.
-~-~~-~~~-~~-~-
Please watch: "Health Workers in Haiti: A Day in the Life"
https://www.youtube.com/watch?v=ZR2l338Kd7o
-~-~~-~~~-~~-~-