The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

Catalog #

168928

Applications

Suitable for use in Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution

Optimal dilutions to be determined by the researcher.

Hybridoma

NS1/AG4.1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Clone Type

Monoclonal

Isotype

IgG1

Clone No

IIB5

Host

Mouse

Concentration

Not determined

Form

Supplied as a liquid in, 0.09% sodium azide.

Purity

Supernatant

Immunogen

BrdU conjugated to bovine serum albumin.

Specificity

Recognizes Bromodeoxyuridine.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.