Statut commercial

Laboratoire

Description

OVERVIEW

The study of molecular interactions dynamic is a key point towards the understanding of regulation mechanisms of the living, as well as their deregulation occurring during neurodegenerative, metabolic or auto-immune diseases. For this, the visualization and measurements of inter-proteins interactions are indispensable in the cellular level.
Recently, fluorescence lifetime measurements coupled with the use of GFP variants (2008 Nobel Prize in Chemistry) are a new major way of investigation based on the mechanisms of energy transfer between fluorescent labels (FRET).
The BCF research group at the IRI has a unique expertise in this field in France. It has elaborated a system enabling to couple the measurements of lifetime and fluorescence spectrum (SLiM). In order to simplify the analysis of this kind of multidimensional data, we have also developed an innovative software based on the polar representation method.

DESCRIPTION

MAPI v1.0 is an image analysis software for your FLIM and SLiM acquisitions. It is adapted to different file formats: .acs (text images), .sdt (Becker & Hickl) and .fli (Lambert Instruments). It calculates and displays automatically a polar representation of your lifetime images. For SLiM images, it enables also to display a 3D polar representation. Finally, it gives you access to different unknown parameters which are the lifetimes and contributions of each fluorescent species.