ELISA: This antibody can be used as capturer in Sandwich ELISA for quantitative determination of HCG in combination with HRP conjugated anti-HCG β subunit antibody (Cat No: ARG10180).

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form

Liquid

Purification

Protein G affinity purified

Buffer

0.01M PBS (pH 7.2)

Storage instruction

For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by placenta after conception. hCG is comprised of a α subunit with 92 amino acids and a β subunit with 245 amino acids. The α subunit is identical to that of LH (Luteinizing hormone), FSH (Follicle-stimulating hormone) and TSH (Thyroid-stimulating hormone). hCG sustains corpus luteum, stimulats progesterone secretion, hence is critical to early foetus development. It has also been reported that hCG plays a role in the development of foetus’s tolerance to maternal immunity. hCG is the most sensitive biomarker for pregnancy.

Function

Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. [UniProt]