Cre recombinase expression can result in phenotypic aberrations in plants

Cre recombinase expression can result in phenotypic aberrations in plants
Coppoolse, Eric; de Vroomen, Marianne; Roelofs, Dick; Smit, Jaap; van Gennip, Femke; Hersmus, Bart; Nijkamp, H.; van Haaren, Mark
2004-10-17 00:00:00
The cre recombinase gene was stably introduced and expressed in tomato, petunia and Nicotiana tabacum. Some plants expressing the cre gene driven by a CaMV 35S promoter displayed growth retardation and a distinct pattern of chlorosis in their leaves. Although no direct relation can be proven between the phenotype and cre expression, aberrant phenotypes always co-segregate with the transgene, which strongly suggests a correlation. The severity of the phenotype does not correlate with the level of steady-state mRNA in mature leaves, but with the timing of cre expression during organogenesis. The early onset of cre expression in tomato is correlated with a more severe phenotype and with higher germinal transmission frequencies of site-specific deletions. No aberrant phenotype was observed when a tissue-specific phaseolin promoter was used to drive the cre gene. The data suggest that for the application of recombinases in plants, expression is best limited to specific tissues and a short time frame.[12pt]
http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.pngPlant Molecular BiologySpringer Journalshttp://www.deepdyve.com/lp/springer-journals/cre-recombinase-expression-can-result-in-phenotypic-aberrations-in-2TrPva3vzA

Cre recombinase expression can result in phenotypic aberrations in plants