Preparation of RNA samples for NGS Described here is a library preparation service only. Once the library is prepared, you will also need to request (and pay for) the sequencing of that library. Total RNA is input into the workflow where mRNA is isolated using oligo d(T), cDNA is made with reverse transcriptase. The cDNA is end-repaired A tailed and Illumina adapters are ligated. The resultant library is then PCR amplified to have enough material for the sequencer. *Illumina has discontinued this kit option*