Dear Andre,
> I am looking for a stain specifically for Mast cells. We have been
> doing the Bismarck Brown for some time, but unfortunately no
> literature available. Surely somebody might just be able to help.
> Thanks a million in advance, I am looking forward to hear from all of
> you.
>
Most Mast cells stain metachromatically, so a toluidine blue (0.1%
aqueous) will do the trick. Allow stained sections to air dry prior
to coverllipping (ie no alcohol). Churukian has a marvelous technique
that was published some time ago in J Histotechnology.
Aqueous alcian blue (1% in 0.5% HCl) (pH 1) will also stain them.
This is mainly due to their being rich in heparin sulphate. Try one
of the Sigma dyes - they tend to dissolve better at this pH.
My histonet summaries contain a page on Mast cell controls which may
be of use.
Regards, Tony
.
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital
Sydney, AUSTRALIA
http://www2.one.net.au/~henwood
http://www.pathsearch.com/homepages/TonyHenwood/default.html