Female Hormone Panel: Patient Overview

The Cyclical Pacing of the Ovaries

The human ovary releases its hormones in a cyclical manner, which is referred to as the menstrual cycle. The average
cycle is 26-30 days. The timing and pacing of hormone release is governed by GnRH, FSH and LH, hormones from the
hypothalamus and pituitary glands in the brain. The two major classes of ovarian hormones released during the menstrual
cycle are estrogens and progestogen, also known as the female steroid sex hormones. The major and most active estrogen
released is estradiol, while the major progestogen is progesterone.

The Female Cycle has Three Physiologic Phases:

1. The follicular phase starts with the onset of menstrual blood flow and is of variable length. This phase is
normally characterized by both low estrogen and progesterone output.
2. The ovulatory phase is 1 to 3 days long, and the human ovum (egg) is released in this phase. This phase is preceded
by high estrogen and LH levels.
3. The luteal phase is rather constant in length, 12-14 days, and ends with menses. In contrast to the follicular phase,
the luteal phase is characterized by high progesterone concentrations and a moderate increase in estrogens.

Functions of Female Sex Hormones

Estradiol and Progesterone affect several target organs involved in successful conception and pregnancy. Additionally,
these hormones maintain a number of secondary sexual characteristics, such as reduced body hair, soft skin texture, a
higher voice pitch and possible release of certain pheromones.

Target Organs of Estrogen and Progesterone

The Uterus - Estradiol prepares the uterus for conception, produces a threefold to fivefold increase
in the thickness of its inner lining and also promotes uterine gland development and mucus secretion. Progesterone causes
a swelling of the uterine lining, an increase in glycogen (a complex sugar) content and an increase in the mucus secreted
by the uterus. If conception does not occur, the uterine lining is shed, resulting in the menstrual flow.

Fallopian tubes - Sex hormones stimulate the Fallopian tubes to move the egg toward the uterus.
The Fallopian tubes also secrete fluids that nourish the egg, the sperm and ultimately the embryo when fertilization occurs.

Vagina - Estrogens promote the thickening of the vaginal lining and increase its secretions, which makes
the lining more resistant to infections.

Breasts - Before puberty, the breasts grow only in proportion to the rest of the body, but under the
influence of estradiol and progesterone during puberty, the breasts develop to maturity. During the menstrual cycle, excess
estradiol causes breast swelling and tenderness.

Bones - In the adult female, Estradiol and Progesterone play an important role in the inhibition of
osteoporosis and improve the incorporation of calcium and magnesium into bone. This is often why doctors prescribe Estrogen
for menopausal women.

Kidney - Estrogens cause the body to retain sodium, which results in fluid buildup. Conversely,
progesterone causes a loss of excess sodium and retained fluid. Before the period begins, there may be a relative excess
of estrogen over progesterone, which commonly leads to several of the PMS-associated complaints.

Liver - Estradiol has stimulatory effects on liver proteins which may reduce thyroid hormone availability
and increases the risk for cardiovascular disease. Estradiol also slows the process of liver detoxification of various
harmful substances.

Miscellaneous - Estradiol can elevate blood sugar in certain susceptible individuals. Progesterone can
increase the appetite and has a general calming effect on the nervous system, especially at night.

What We Test

The Female Hormone Panel™ is a simple, non-invasive test. Eleven saliva samples are collected during speciﬁed time
periods throughout the menstrual cycle.

Using this panel the ﬁrst time in routine medical practice, both patients and doctors can view the proﬁles of estrogen
and progesterone simultaneously. Diagnostic guesswork is minimized, and hormonal balance evaluation and customized
treatment becomes a reality. In addition, we report the cycle average for testosterone and DHEA. The expanded panel
also includes five FSH and LH measurements to see if brain control and stimulation of the ovaries are optimal.

Applications of the Female Hormone Panel™

Detection of Luteal Phase Deficit There are at least three luteal phase-deficit patterns which are
characterized by a progesterone/estrogen imbalance. This imbalance is usually associated with PMS, infertility, ﬁbroids
and other female hormone-related problems and can be readily detected by our panels.

Hormonal Imbalance and PMS Many women suffer from hormonal imbalance in the estrogen to progesterone
ratio. Our panels can define the hormonal state with exquisite accuracy which, in turn, provides speciﬁc insights for
appropriate intervention to relieve hormonal imbalance and PMS-related symptoms.

Customized Hormone Therapy Presently, female hormone therapy is not individualized to the needs of
each woman, because current diagnostic tests do not provide sufﬁcient data. Consequently, most women are empirically
treated without regard to their individualized physiology and speciﬁc needs. The FHP™, for the ﬁrst time, will allow
a clinician to customize therapy to each female patient.

Other Applications

The FHP™ can also be used to detect and monitor the following:

Functional infertility

Influence of diet, exercise and other lifestyle factors on the cycle

Menstrual problems originating in the brain

Early pregnancy problems, such as spontaneous miscarriage

Cycle irregularities, following the use of birth control pills

Dysmenorrhea, that is painful and heavy periods

Migraine headaches

Cystic ovarian disease

Early osteoporosis

Advantages of the FHP™

Convenience: Requires no blood draws, therefore no repeat clinic visits, and avoids the inconvenience
of the 24-hour urine collection.

Economy: The fee for the 11-sample test is less than that of two blood determinations or a urine
analysis for estrogen and progesterone.

Physiological accuracy: Research has demonstrated that the free hormone fraction predominates in
saliva. Hormones can be found free or bound to protein. The free hormone fraction is very important, because it is
the bioactive fraction that most significantly influences living cells. The salivary female hormone levels correlate
at 93% with the free hormones in the tissues.

One or two blood determinations or a 24-hour analysis of urine for these hormones gives only an idea of the ovaries’
hormonal productivity. The FHP™ provides a view of the ovarian capacity over a whole menstrual cycle.

Do you need the FHP™?

To determine if the FHP™ is the appropriate test for your hormone-related problems, consult with your physician or
healthcare provider.

If either infertility or PMS are of concern to you, ask your doctor about the Expanded Female Hormone Panel™.

Our expanded panel not only reveals any hormonal imbalances, but also accurately indicates the basis of the problem.