HyStem-C®, Hydrogel Kit

Price(USD):$316.94

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BMGS313-1EA

HyStem-C®, Hydrogel Kit

EA

1 Kit

$316.94

Introduction:

HyStem-C™ is recommended for stem cell researchers as a starting point for optimization of a cell’s microenvironment since it contains Gelin-S. Gelin-S, or thiolated gelatin (denatured collagen), allows attachment of a wide variety of cell types and takes the guesswork out of the appropriate attachment factors to use.

HyStem-C™ provide an excellent starting point for optimizing the matrix for stem cell culture.since its composition can be easily tailored to find suitable matrix compositions. Unlike an animal-derived extracellular matrix (ECM), HyStem-C™ is fully chemically defined. The hydrogels are based on three biocompatible components: thiol-modified hyaluronan (a major constituent of native ECM), thiol-modified gelatin (denatured collagen), and a thiol-reactive crosslinker, polyethylene glycol diacrylate (PEGDA). HyStem-C™ hydrogels can be customized by adding ECM proteins and by varying the hydrogel compliance to match the stiffness of native tissues.

Gelation

Reconstituted HyStem-C™ components remain liquid at 15 to 37°C. The hydrogel is formed when the crosslinking agent, Extralink™ (PEGDA) is added to a mixture of Glycosil™ (thiol-modified hyaluronan) and Gelin-S™ (thiol-modified gelatin). Gelation occurs in about twenty minutes after all three components are mixed. No steps depend on low temperatures or low pH. Diluting the components with phosphate-buffered saline (PBS) or cell-culture medium can increase the gelation time.

Volume and Composition

The HyStem-C™ Hydrogel Kits comes in one size:

7.5 ml total hydrogel with three sets of vials that make 2.5 ml each (for small-volume applications)