Cell number counting in preimplantation embryos
(P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)A simple procedure for determining cell number in preimplantation embryos. While the procedure is written for fixed embryos, fixation is optional since the Hoechst 33342 and 4',6-diamidino-2-phenylindole, dihydrochloride (DAPI) penetrate living cells. The fixation step is useful when embryos will be stored for several weeks before determination of cell numberhttp://www.animal.ufl.edu/hansen/protocols/embryoc...Added: Tue May 14 2002, Hits: 1453, Reviews: 0Write reviewCached

Immunolocalization of CDX2 in Bovine Embryos – A Method for Differential Counting of Trophectoderm a
(PJ Hansen Laboratory, University of Florida)For the current procedure, we take advantage of the fact that the transcription factor CDX2 is localized exclusively to trophectoderm (TE) to perform differential cell counting. Cells that are labeled with antiCDX2 and a nuclear dye (here DAPI but others can be used also) are considered TE whereas labeled nuclei that are negative for CDX2 are considered inner cell mass. The immunodetction method can be used for other antigens also. http://www.animal.ufl.edu/hansen/Protocols/Immunod...Added: Wed Jun 15 2011, Hits: 447, Reviews: 0Write review

Mini-Chamber for regulating gaseous environment during culture
(P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)A simple and inexpensive chamber for regulating gaseous environment of small culture plates, such as those used for culture of preimplantation embryos, can be constructed using disposable media-filtration devises such as Corning’s 115-ml system. The following is a description of how to make such a devicehttp://www.animal.ufl.edu/hansen/protocols/minigas...Added: Tue May 14 2002, Hits: 934, Reviews: 0Write reviewCached