制备和贮存

This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

别名

Alteplase

DKFZp686I03148

Plasminogen activator tissue

Plasminogen activator tissue type

PLAT

Reteplase

t PA

T Plasminogen Activator

t-PA

T-plasminogen activator

Tissue plasminogen activator (t PA)

Tissue type plasminogen activator

Tissue-type plasminogen activator chain B

tPA

TPA_HUMAN

TPA1

see all

功能Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.

疾病相关Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.

结构域Both FN1 and one of the kringle domains are required for binding to fibrin.Both FN1 and EGF-like domains are important for binding to LRP1.The FN1 domain mediates binding to annexin A2.The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.

翻译后修饰The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.Characterization of O-linked glycan was studied in Bowes melanoma cell line.