This study examines the structure of the active center of bacterial luciferase by exploiting the existence of two well-documented structural features of the (alpha) subunit of the enzyme, the essential cysteine and the ...

Bacterial luciferase catalyzes the mixed-function oxidation of a long-chain saturated aldehyde and FMNH(,2) to yield the carboxylic acid, FMN and blue-green light. The enzyme is inactivated by 2,4-dinitrofluorobenzene ...

Cytochrome P-450$\sb{\rm cam}$, from Pseudomonas putida, catalyzes the regio- and stereospecific hydroxylation of the monoterpene d-camphor to afford 5-exo-hydroxycamphor as the sole product. The roles of specific active ...

Peptides comprising 80% of the amino acid sequence of the (beta) subunit of bacterial luciferase from Vibrio harveyi were isolated and their amino acid sequences were determined by Edman degradation. The protein was ...

I have investigated signal recognition particle (SRP)-mediated protein targeting using a combination of genetic, biochemical, and molecular sequence analysis techniques. First, I consider the SRP cycle from the perspective ...

Structural and functional properties of wild type and mutant forms of cytochrome $bo\sb3$ quinol oxidase from E.coli were examined. Structural properties of subunit II were addressed with the restoration of the putative ...

Chondrocyte cell cultures originating from the histologically distinct zones of the tibiotarsus synthesize chondroitin sulfate and type II collagen in primary and secondary cultures. The collagen is secreted into two ...

In the first part of this work we provide evidence indicating that under standard cell culture conditions unliganded estrogen receptor (ER) is unable to activate transcription. Studies of Chinese Hamster Ovary cells which ...

The structural units of heparin produced by nitrous acid deamination were stoichiometrically reduced with NaB('3)H(,4) and analyzed by high performance ion exchange chromatography to obtain resolution of the four primary ...

The cytochrome d terminal oxidase is one of two terminal oxidases in the aerobic respiratory chain of E. coli. The topology of the two subunits of the complex were examined by the use of alkaline phosphatase gene fusions, ...

The presence of antifreeze glycopeptides (AFGPs) in the blood of polar fishes allows fish to live in ice-laden seawater at a temperature below what colligative properties would dicate to be the freezing point of their ...

The genes encoding the enzymes for de novo pyrimidine biosynthesis in the gram-positive microorganism Bacillus subtilis were previously shown to be clustered at 139 min on its chromosome, transcribed from a single promoter ...

The induction of the Xenopus estrogen receptor mRNA by its ligand, estradiol-17$\beta$ (E$\sb2$), results in the establishment of a permanent autoregulatory loop. In order to identify the element(s) responsible for the ...