Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

Flow Cyt

Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP

Use at an assay dependent concentration.

RIA

Use at an assay dependent concentration.

IHC-Fr

Use at an assay dependent concentration.

Application notes

Is unsuitable for IHC-P.

Target

Function

The CD3 complex mediates signal transduction.

Involvement in disease

Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.

Thank you for your enquiry regarding ab22.
To our knowledge this antibody has only been tested in immunoprecipitation, radioimmunoassay, immunohistochemistry (Frozen sections)and FACS, we have no record of its use in cell proliferation assays.
If you do come across a reference to this antibody in the literature we would be grateful to receive this information so we can update our datasheet to share this with future users.
Please do not hesitate to contact us again if you need further information,