Fellow netters,
is there any vector available for expression in mammalian cells, that has a
unique NcoI site, that may be used for cloning. Alternatively any vector will
do it, that has no NcoI at all. All vectors I have found so far contain 2-3
NcoI sites somewhere in their sequence, it should not be a problem to insert a
suitable site behind the promoter. I am just afraid of removing all the extra
sites from one of the available vectors.
Thanx
Michael
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* * ****** ******** *********************************************
** ** *** ** ******** * Michael Szardenings *
*** *** ***** *** * Biomedical Center, Pharm. Pharm. *
**** **** ***** *** * Box 591 Tel.:int46-18-17422*
** *** ** ** *** ******** * S-751 24 Uppsala FAX :int46-18-55971*
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INTERNET : MSZ at bmc.uu.se
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