Under authority of the Richard B. Russell National School Lunch Act, the Agricultural Marketing Service (AMS) works with its sister Agency, the Food and Nutrition Service (FNS), to purchase and distribute foods through various Federal nutrition assistance programs. These programs include, but are not limited to, Commodity Supplemental Food Program, Food Distribution Program on Indian Reservations, Temporary Emergency Food Assistance Program and the National School Lunch Program (NSLP), which provides nutritious meals to over 31 million school children in over 100,000 participating public and private nonprofit schools and institutions each year.

The data generated under these testing programs are used to determine the eligibility of vendors to supply food to Federal nutrition assistance programs, and to continually refine and strengthen the microbiological testing requirements for AMS purchase programs. Moreover, summaries of this data provide an overall picture of vendor performance.

Sample Collection and Processing

AMS sampling procedures and frequencies are described in the specification documents of each commodity (Boneless Beef, Ground Beef, Cooked Diced Chicken, and Egg Products). Samples are collected and processed by establishment personnel and tested using laboratory methods specified by AOAC International, the Food Safety and Inspection Service (FSIS) Microbiological Laboratory Guidebook, and/or the Food and Drug Administration (FDA) Bacteriological Analytical Manual. Samples are collected in areas free from air currents on work surfaces that have been cleaned and sanitized. Samples are collected in an aseptic manner assuring that no foreign sources of microbial contamination are introduced. Tools used for sampling are sterilized before use and protected from outside contamination during use. Samples are then placed into sterilized containers and placed in refrigerators or freezers. Refrigerated samples are shipped to laboratories on ice in insulated containers, while frozen samples are shipped packed in dry ice in insulated containers using overnight courier service. Samples are identified by FSIS establishment number, production/sample date, and lot/sublot number(s).

Laboratory Analysis

AMS contracts with commercial laboratories to perform microbiological testing of its commodities. As part of the contractual process, AMS does in-depth audits of the laboratories to ensure they are capable and equipped to conduct the necessary analyses. Each laboratory is audited annually by representatives of the AMS Science and Technology program. Audit specifications include accreditation to International Standards Organization (ISO) 17025 and participation in the American Proficiency Institutes or similar sample proficiency program. Corrective actions are required to remedy any deficiencies identified during audits and subsequent confirmation of the implementation of corrective actions is necessary to gain final audit approval.

Microbiological Testing Results

Boneless Beef

Boneless beef produced by AMS vendors is sampled and tested for the following indicator microorganisms: standard plate count (mesophilic aerobic plate counts), total coliforms, and generic E. coli, with critical limits of 100,000 colony forming units (cfu)/gram (g), 1,000 cfu/g, and 500 cfu/g, respectively. Any lot of product found to exceed any of these control limits is rejected for purchase by AMS.

Ground beef produced by AMS vendors is sampled and tested for the following indicator microorganisms: standard plate count (mesophilic aerobic plate counts), total coliforms, and generic E. coli, with critical limits of less than 100,000 cfu/g, 1,000 cfu/g, and 500 cfu/g, respectively. Any lot/sub-lot of product found to exceed any of these control limits is rejected for purchase by AMS.

Cooked diced chicken produced by AMS vendors is sampled and tested for the following indicator microorganisms: standard plate count (mesophilic aerobic plate counts), total coliforms, generic E. coli, and Staphylococcus (S.) aureus, with critical limits of 1,000 cfu/g, 50 cfu/g, 10 cfu/g, and 10 cfu/g, respectively. Any lot of product found to exceed any of these control limits is rejected for purchase by AMS.

All purpose egg mix and frozen pasteurized whole eggs produced by AMS vendors are sampled and tested for the following indicator organisms: standard plate counts (mesophilic aerobic plate counts) and total coliforms, with critical limits of 1,000 cfu/g and 10 cfu/g respectively; and for Salmonella, with zero tolerance.