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1A, C). In contrast, sea stars injected with 6 g l−1 Oxgall ( Fig. 1B) only experienced 80% mortality (FET1, N=40; p = 0.053). While there was a significant difference between Bile Salts No. 3 and Oxgall in the overall proportion of sea stars BMS-354825 that died within 2–3 days (FET1, N=50; p = 0.011) the concentration of these chemicals had no apparent effect

on the proportional mortality of injected sea stars ( Fig. 1C, D; FET1, N=10; p = 0.053). Six out of 25 Sea stars that were injected with oxgall initially exhibited signs of the effects of bile injections (i.e. loss of turgor and localized lesions at the site of injection) within the first 24 h, but ultimately recovered after 7 days of observation. Even when Oxgall concentrations

were doubled, mortality rates were at 60% (3 out of 5). The time until death was significantly affected by both the substance used and the dose (F1,32 = 4.335, p = 0.045; Table 3); COTS injected with oxbile N3 died in 28.95 h ± 4.08SE compared to 57.98 h ± 12.95SE for those injected with Oxgall. Time selleck chemicals until death was also substantially reduced by doubling the dose of each of the bile derivatives: At 8 g l−1 of oxbile N3, all A. planci died within 24 h ( Fig. 1C), whereas at 4 g l−1, some individuals persisted for over 48 h ( Fig. 1A). The differences observed between oxgall and oxbile N3 could be related with the fact that oxbile N3 is composed of sodium cholate and sodium deoxycholate that are two well known detergents that lyse NADPH-cytochrome-c2 reductase cell membranes after contact. After 8 days of exposure to dead A. planci injected with the higher concentration of Bile Salts No. 3 (8 g l−1), and despite complete consumption of sea stars remains, none of the fishes, or corals exhibited any signs of ill-health. The remains of the sea stars were consumed mainly by the pufferfishes (Arothron spp.), but also triggerfishes (Balistoides viridescens), butterflyfish (Chaetodon auriga) and damselfishes (Pomacentrus moluccensis). Most notably, each individual pufferfish (Arothron spp.) consumed up to 0.9 A. planci during

the course of this experiment. Oxbile contained within the tissues of dead and dying A. planci is likely to be readily decomposed by free-living marine bacteria ( Maneerat et al., 2005), thereby reducing the amount of bile ingested by fishes, especially when feeding on the remains of sea stars that have been dead for hours to days. There were no adverse effects on behavior or health following substantial ingestion of A. planci killed using Bile Salts No. 3 at 8 g l−1. These findings support observations made during similar trials conducted in the Philippines ( Rivera-Posada et al., 2013). However, the fishes used in the current experiment (especially, pufferfishes and triggerfishes) were generally smaller than those caught in the Philippines (using fish cages), such that any toxic effect from ingestion of oxbile would be expected to have been even more apparent.

05% Tween-20, PBST). After blocking with blocker A from MSD for 1 h, the plates were probed with Bortezomib purchase 50 μL of samples that were diluted 1/50 in sample diluents supplemented with 10% fetal

calf serum (FCS) and incubated for 90 min. SULFO-TAG conjugated secondary antibody against human immunoglobulin G (IgG, MSD, Gaithersburg, MD) was diluted 1/5000 and used to quantitatively measure the presence of each autoantibody. Electrochemiluminescence signal was quantified on the SECTOR Imager 6000 reader immediately after 150 μL of MSD Read Buffer T (containing surfactants and tripropylamine as a coreactant for light generation) was loaded in each well. Samples collected under different conditions were run in duplicate on one plate and raw signals Veliparib were used for data analysis. The 12 protein biomarkers that constitute the MBDA test were measured using analyte-specific capture and detection antibodies. Briefly, multi-spot 96-well plates were coated with analyte-specific capture antibodies

also known as YKL-40). Dilutions for panels A, B and C were 1/2, 1/1000 and 1/20, respectively. Fifty microliters (for panels A and C) and 25 μL (for panel B) of standard, blank, control, or sample were added to the appropriate well in the 96-well plate. The plates were incubated for 120 min with continuous shaking at 750 rpm and then washed 3 times in PBST wash buffer. Twenty-five microliters of prediluted blends of SULFO-TAG conjugated Ergoloid detection antibodies was added to each well. Following incubation with the detection antibody blend for 60 min, plates were washed again, and upon adding 150 μL of read buffer, the electrochemiluminescence signal was quantified as in Section 2.2.3. MSD Discovery Workbench calculates the four-parameter logistic regression curve fits (Findlay and Dillard, 2007) for each standard curve and determines concentrations for all samples. The concentration of the samples was used for further comparison of results obtained with different sample collecting/handling processes. The MBDA algorithm was developed in a separate series of studies and clinically validated in an independent cohort (Curtis et al., 2010) using the DAS28-CRP as a gold standard (Prevoo et al., 1995 and Inoue et al., 2007). Derivation and clinical validation of this algorithm are reported elsewhere (Curtis et al., 2010).

0, 100 μM), hydrogen peroxide (3 mM) and NADH (50 μM) in 10 mM phosphate buffer (pH 7.4) were incubated in the presence or absence of Cu(II) sulphate or Cu(II)–imine complexes (50 μM) in order to assay the generation of oxygen-derived radicals with the capacity to bring about the one-electron oxidation of NADH generating

complexed with imines or Gly-derived ligands (50 μM) in 50 mM phosphate buffer (pH 7.4) were incubated at 37 °C for 1 h. A 500 μL aliquot of 1% (w/v) 2-thiobarbituric acid was then added, the solution was heated to 100 °C for 15 min, and allowed to cool, and the absorbance was read at 532 nm (ε = 1.36 × 105 M− 1 cm−1). Human neuroblastoma cells SH-SY5Y were purchased from the American Type Cell Culture (ATCC) and incubated in Dulbecco’s MEM-F12 medium supplemented with 10% foetal calf serum (FCS) at Neratinib datasheet 37 °C in an atmosphere of 5% CO2 in air. In order to treat cells with Cu(II) complexes with Gly-derived ligands, fresh solutions containing 12 mM Cu(GlyGlyGly), Cu(GlyGlyGlyGly) or Cu(GlyGlyHis) were used to prepare MEM-F12/FCS medium supplemented with 50 μM of complex. This concentration was chosen for all experiments since it allowed reasonable Isotretinoin cell growth at all incubation times investigated. Experimental

cells were plated at a density of 4 × 104/cm2 and incubated at 37 °C in an atmosphere of 5% CO2 in air. Following incubation, cells were trypsinised and adherent cells combined, washed with phosphate buffered saline [PBS; containing potassium chloride (2.7 mM) and sodium chloride (137 mM) in 10 mM phosphate buffer (pH 7.4)], stained with Trypan blue and counted under the optical microscope using a Newbauer’s chamber. Human neuroblastoma cells SH-SY5Y was incubated in Dulbecco’s MEM-F12 medium supplemented with 10% foetal calf serum (FCS) at 37 °C in an atmosphere of 5% CO2 in air. In order to treat cells with Cu(II) complexes with Gly-derived and imine-derivative ligands, fresh solutions containing 12 mM Cu(II) complexed with imines or Gly-derived ligands were used to prepare DMEM-F12/FCS medium supplemented with 50 μM of complex. Experimental cells were plated at a density of 4 × 104/cm2 and incubated at 37 °C in an atmosphere of 5% CO2 in air, in distinct triplicate experiments. Following incubation, cells were trypsinised and adherent cells combined, and washed 5 times with phosphate buffered saline [PBS; containing potassium chloride (2.7 mM) and sodium chloride (137 mM) in 10 mM phosphate buffer (pH 7.4)] containing EDTA 1.

In some shallow areas, the bloom was hard to recognize due to shallow bottom or/and the presence of suspended sediments, as revealed by the bright feature in the ERGB images. Since late February 2009, the bloom patch began to move toward the Strait of Hormuz and out into the Gulf of Oman.

The satellite image collected on February 27 2009 showed that the bloom patch extended from the Strait of Hormuz to almost over the entire Gulf of Oman. This may be caused by the convergence of two bloom patches, one flowing out of the Strait of Hormuz from the Arabian Gulf and the other flowing northward from the Arabian Sea. This spatial distribution pattern remained till early April 2009. Since late April 2009, the bloom patch moved back into selleck the Arabian Gulf again. From May to late June 2009, the bloom patch was mainly found along the western coast of UAE to the Strait of Hormuz, and in the eastern Gulf of Oman. From late July 2009 on, the bloom patch shrank gradually. In late August 2009, the bloom patch was gone. Although

areas where the bloom patches were found in previous images had no valid satellite-derived chlorophyll-a data on August 30 2009, examination of all images one month after August 30 2009 indicated no suspicious features. Fig. 4 shows the surface current vectors for dates corresponding to one day before those presented in Fig. 2 and Fig. 3. The movement patterns of bloom patches agreed well with numerical model results. These observations are in good agreement with previous similar studies where satellite observations ifoxetine3-MA order were found to be a

valuable source of information to track the dynamic of red tide blooms over large areas (Hu et al., 2011 and Zhao et al., 2013). Being aware of the initiation process and spatial dynamic of red tide blooms can be profitable for biogeochemical forecasting models and provide evidence and operational guidelines for future decision-making mechanisms and emergency response actions. However, identifying the sources of nutrient supply to support and maintain blooms is not straightforward and has always posed a challenge to researchers. Since the outbreak of the 2008 bloom did not coincide with any record of large river discharges (Nezlin et al., 2010) and the freshwater inputs are low in the studied region, the bloom must have been initiated by other non-fluvial sources. Richlen et al. (2010) suggested that the bloom may be related to physical forcing in the Arabian Sea, such as convective mixing. To investigate the potential role of physical forcing in triggering the 2008 bloom, surface ocean circulations from a HYCOM model were examined for the period preceding the first detected bloom patch observed on August 26 2008 (Fig. 2). The ocean circulation results indicated that the flow fields were upwelling favorable from August 7 onward. One example is shown in Fig. 5a.

The solution field is then evaluated at the vertex in the post-adapt mesh using the finite-element basis functions of the containing element in the pre-adapt mesh. Consistent interpolation is bounded (for linear basis functions) but is non-conservative and is only well-defined for continuous function spaces. The second method uses the

intersection of the pre- and post-adapt meshes to form a supermesh. The fields are then interpolated via the supermesh using Galerkin projection ( Farrell et al., 2009 and Farrell and Maddison, 2011). By construction, it is conservative, but is not necessarily bounded. Alisertib datasheet Any overshoots or undershoots in the solution field that occur are corrected, essentially by diffusing the deviation from boundedness. The diffusion introduced in this approach is minimal when compared

with consistent interpolation ( Farrell et al., 2009). This bounded, minimally Venetoclax in vivo diffusive, conservative method will be referred to as bounded Galerkin projection. Different methods for interpolation from the pre- to post-adapt mesh have a less significant impact on the adaptive mesh simulations than that of the metric (Hiester et al., 2011 and Hiester, 2011). The majority of simulations presented here use consistent interpolation for both the velocity and temperature fields as, for this numerical configuration, it provides a faster method than bounded Galerkin projection (Hiester et al., 2011). The final adaptive mesh simulations considered for the comparison with Özgökmen et al. (2007), Section 5.5, use consistent interpolation for the velocity field and bounded Galerkin projection for the temperature

field as improved results for the initial set-up have been obtained with this combination (with a reduction in the mixing of approximately 7% at later times, Hiester, 2011). The meshes are adapted every ten time steps. This choice of adapt frequency provides a balance between being sufficiently frequent so as to prevent features propagating out of the regions of higher mesh resolution and hence deteriorating the solution but not so frequent as to notably increase the computational overhead (cf. Hiester et al., 2011, and Section 3.4). The minimum and maximum edge lengths are set to 0.0001 m and 0.5 m, respectively Methisazone and the maximum number of vertices is set to 2×1052×105, which is comparable to the medium resolution fixed mesh, Table 2. The meshes are adapted to the horizontal velocity field, vertical velocity field and the temperature field with solution field weights denoted ∊u∊u, ∊v∊v and ∊T∊T, respectively. For M∞M∞ two sets of solution field weights are considered, Table 3, following the values of Hiester et al. (2011). The first set are spatially constant. The second set has spatially constant values of ∊v∊v and ∊T∊T and a value of ∊u∊u that varies exponentially in the vertical such that the value at the top and bottom boundaries is two orders of magnitude smaller than that at the centre of the domain, Table 3.

oryzae from a 2012–2013 Arkansas collection, a fast and simple procedure was developed to prepare DNA for PCR amplification. The procedure included two steps: (1) M. oryzae-inoculated filter paper pieces were stored for a minimum of 5 months at –20 °C and transferred to 100 μL of TE (10 ×, pH 7.5, Tris and EDTA) in a 0.5-mL Eppendorf tube using a sterile loop ( Fig. 1). The tube was then incubated in a thermocycler at 95 °C for 10 min, and (2) after SB203580 incubation, the tube was spun for 1 min at 3000 r min− 1 to prepare the DNA for PCR. The PCR reaction was modified as follows. Instead of 1 μmol L− 1 of primer in the final PCR reaction, 2.5 μmol L− 1 of primer was used to increase reproducibility

and the success rate of PCR amplification. To evaluate the quality and stability of the extracted DNA, 1 μL was repeatedly used throughout the PCR tests on the extraction day and on days 4, 8, 10, and 18 of refrigerated storage (Fig. 2). Predicted PCR products were amplified

from fungal structures maintained on filter paper, and from DNA prepared by a conventional procedure as a control (Fig. 2). Isolates that did not yield predicted PCR products were confirmed by PCR amplification using another primer, AVR9-YJ that is specific to the www.selleckchem.com/products/SB-431542.html coding region of the same gene (Fig. 2-D). However, the presence of AVR-Pi9 in isolates 12, 13, 14, and 28 was undetermined ( Fig. 2-D). The same set of DNA was also tested using primers YL149/YL169, confirming the presence of AVR-Pita1 in 15 isolates. Again the four isolates in which AVR-Pi9 was not amplified showed no amplification of AVR-Pita1, suggesting problems with the fungal structures or their DNA quality for PCR ( Fig. 2-E). Gene detection using PCR is a common method of microbial identification and diagnosis. Although PCR amplification can be directly performed using various microbial cultures, prior isolation of DNA is often Dapagliflozin preferred. The DNA extraction process eliminates unknown interfering substances and appears largely to ensure consistent

test results. Toward this end, considerable efforts have been made to improve DNA preparation from fungi [6], [7], [8], [13] and [14]. Many of these methods rely on using a grinder (with or without liquid nitrogen) to break up the mycelia. However, this is a time-consuming task when large number of samples are to be processed. In the present study, the whole procedure can be completed within 11 min at the cost only of TE buffer for sample preparation. It works by disrupting the cell wall and releasing DNA using a high temperature, 95 °C, into a highly concentrated TE solution for 10 min. It is important to note that some samples failed to yield PCR products when only 1 μmol L− 1 of each primer was used (data not shown). However, 2.5 μmol L− 1 of primer was able to ensure successful PCR amplification for most of the samples tested.

Then, 25 μL of the sample or control was added to each well, and the plate incubated at room temperature for 1 h with gentle mixing, to allow the immobilized velaglucerase alfa to capture any antibodies present. Each well was washed three times with 300 μL wash buffer to remove unbound proteins. Next, 25 μL per well (1 μg/mL) anti-human secondary antibodies against the human IgA, IgM, or IgE domain labeled with ruthenium complex were added, and incubated at room temperature for 1 h with gentle mixing, resulting in the formation of an Ig class-specific

complex with any bound anti-velaglucerase alfa antibodies. Each well was washed three times with 300 μL of wash buffer to remove unbound labeled secondary antibody. Then, 150 μL of diluted read buffer “T” was added to each well, Selleckchem CH5424802LDK378 price and the plate was read immediately with the Sector™ MSD 2400 instrument, as described for the screening assay. Samples were prepared as a 1/20 dilution using 2% Blocker B, 1.5 M NaCl, and 0.05% Tween 20 in PBS. Normal human serum was used as a negative control, prepared as a 1/20 dilution in dilution buffer. Three levels of artificial antibody-positive controls spanning the dynamic ranges of these assays were prepared since

anti-velaglucerase alfa or anti-imiglucerase IgA, IgM, or IgE antibodies were not available. Neutralizing antibodies (NAb) interfere with the biological activity of the enzyme they bind. This assay was designed to detect the presence in human serum of NAb that interfere with velaglucerase alfa or

imiglucerase activity in vitro, using an assay to both detect and quantify antibodies that inhibit enzyme activity. Exoribonuclease The method is based on a colorimetric activity assay that measures the ability of velaglucerase alfa and imiglucerase to hydrolyze the synthetic substrate 4-nitrophenyl-β-d-glucopyranoside to p-nitrophenol and d-glucopyranoside. The method described was identical for imiglucerase antibodies, substituting imiglucerase for velaglucerase alfa wherever written. Firstly, diluted serum samples or assay controls were mixed with an equal volume of 500 ng/mL of velaglucerase alfa (final concentration 250 ng/mL) in a microdilution tube. The mixtures were then incubated at 37 °C for 30 min with constant shaking. After incubation of serum/enzyme mixtures, 20 μL of each sample or assay control was added to the bottom of the wells in 96-well Maxisorp® microtiter plates. 80 μL of 10 mM substrate solution (10 mM substrate [4-nitrophenyl-β-d-glucopyranoside] solution in 20 mM citric acid/40 mM sodium phosphate/0.1% Triton X-100/2.3 mM taurocholic acid, pH 5.5) was quickly added to each well. For the enzymatic reaction to occur, the plate was incubated at 37 °C with gentle shaking for 1 h. After incubation with substrate, 150 μL of stop solution (0.3 M glycine/0.2 M sodium carbonate, pH 10.7) was quickly added to all wells, beginning with the wells for the p-nitrophenol calibration curve.

Finally, 16 educational sessions were held to inform all MICU nurses regarding sedation-related issues within the QI project. Third, http://www.selleckchem.com/products/ldk378.html execution of the project during the 4-month QI period involved the following steps: 1 Modifying the standardized MICU admission orders to change the default activity level from “bed rest” to “as tolerated. Fourth, evaluation of the project occurred on an ongoing basis during the QI period via weekly meetings of the multidisciplinary QI project team to discuss progress, barriers, and solutions. For all patients included during

the 3-month pre-QI period18 and the 4-month QI period, data from paper and electronic medical records were abstracted, and PR-171 order relevant evaluations were completed as described in the following paragraphs. Patient baseline data including demographics, comorbidities (including the Charlson Index24), and severity of illness at ICU admission were obtained from the medical record. For included patients, the following data were collected on a daily basis while in the MICU: (1) benzodiazepine and narcotic drug doses received (converted to midazolam- and morphine-equivalent doses, respectively, using standard conversion factors25 and 26), (2) sedation

and delirium status (evaluated using the validated Richmond Agitation-Sedation Scale23 and Confusion Assessment Method for the ICU27 instruments, respectively), and (3) patient pain status (based on MICU nurses’ routine clinical assessments using a standard 0–10 scale, with a CYTH4 higher number representing greater pain). The number of PM&R-related consultations and treatments occurring while each patient was in the MICU was collected. In addition, daily functional mobility activities conducted by PT and OT were recorded by the therapist using standard categories from prior related research.12 “Unexpected events” occurring during PT and OT (defined as cardiopulmonary arrest, loss

of consciousness, fall, removal of any medical device, or oxygen desaturation <85% for >3 minutes) were prospectively evaluated with each treatment. In order to evaluate any overall impact of the QI project across all MICU patients, hospital administrative data were evaluated. Specifically, the number of PT and OT consultations and treatments and the number of admissions and LOS for all patients receiving care in the MICU during the 4-month QI period and the same period in the prior year were obtained from by the Departments of PM&R and Medicine, respectively. Descriptive statistics including proportions (for binary and categorical data) and medians with interquartile range (for continuous data) were used to summarize individual patient-level data and the data collected on a daily basis during patients’ MICU stay.

Well-designed (perhaps cluster-randomized) controlled trials are needed to test the generalizability of these results and to build evidence for best practice in this area. Effective, simple, nonpharmacological interventions have the potential to improve the residential care environment at little cost, while reducing negative dementia-related behaviors and improving selleck chemicals the mealtime environment. We thank Dr Ukoumunne for clarification on the statistics involved with certain included studies. ““In 1982 I was invited to spend a month in Tsingtao (now Qingdao), Shantung Province, northeast China. My host was the First Institute of Oceanology – China’s

premier marine http://www.selleckchem.com/products/BEZ235.html institute – and I was going to investigate, illustrate and write up the marine life of the rocky shores around the institution and, in the process, teach local students something of shore ecology. I also wanted to compare that temperate/boreal ecology with that of Hong Kong’s subtropical. There are many stories I could relate about the month’s sojourn in Tsingtao, but one stands out. Working one day on the rocky shore right in front of the institute, I was joined by a grizzled

granddad with his granddaughter – the latter about four I would guess although maybe she was older. And I could tell that they were both obviously under-nourished, the little girl with spindly

legs and a potbelly. With my (very) few words of Putunghua we communicated and, afterwards, I watched as they gleaned their way along the shore picking up the smallest of crabs (Gaetice, Hemigrapsus), mussels (Xenostrobus), gastropods (Thais), even Ligia, which the little girl was 3-oxoacyl-(acyl-carrier-protein) reductase especially adept at catching. They were going to make a soup with what they had found. This, remember, was just post-Red Guard Cultural Revolution and the country was on its knees. One thing struck me though: what I was seeing on these shores and would eventually describe ( Morton, 1990) was not natural. It was as impacted ecologically as any polluted beach in the modern world. This was the first time I understood not just the meaning of poverty but also the impact of ordinary people on marine life and on our interpretation of ecology. I returned, chastened, to Hong Kong where such intertidal gleaning was, generally, no more and, certainly not a necessity. Here, the problem was simply one of pollution although I had mentally re-defined and broadened the meaning of that word. I now jump forward nearly ten years. In 1989, the Swire Marine Laboratory (now the Swire Institute of Marine Science) of the University of Hong Kong was founded and, deliberately, situated on the remotest peninsula, Cape d’Aguilar, on Hong Kong Island.

For closure using 1BA-MCTS, a single-sided balloon (20-mm expanded diameter) expanding only in the opposite direction of endoscopic view was attached to the contralateral side of DBSS. The balloon was inflated near the perforation site to expand the collapsed gastric wall; however, the limited bidirectional expansion together with DBSS shaft could not obtain a sufficient

operative field (Figure 2B). For closure using the 2BA-MCTS, DBSS and the 2 balloon arms were attached at the apices Oligomycin A mw of an equilateral triangle, which enabled the expansion of the operation field at 3 points, allowing clear view of perforation site. Even in the collapsed stomach, expanding the operative field at 3 points allowed en face visualization of the perforation site without insufflation, and the appropriate expansion strength enabled accurate suturing bite and pitch (Figure 2C). After 6 stitches were taken, the first arm was inserted into the remaining 6-mm perforation site and suturing continued; however, retraction of the first arm back into the stomach was very difficult. Therefore, further suturing was performed using the mini-DBSS. The mini-DBSS has a small arm on 1 end, and the back-and-forth movement of the second arm allows full-thickness suturing of narrow perforation of the gastric wall. It has the same basic structure as the DBSS. The 30-mm perforation was

sutured using 7 stitches with 4-mm pitch and bite, performed using DBSS and mini-DBSS. In addition, to strengthen the closure, 2 mucous membrane purse-string sutures were performed using the mini-DBSS. Finally, we PF-02341066 manufacturer conducted an air leak test. In Video Clip 2, we performed in vivo EFTR experiments on female Beagle dogs of 30-mm diameter hypothetical lesions in the lesser curvature of the lower body (Figure 2D), C-X-C chemokine receptor type 7 (CXCR-7) the anterior wall of the middle body ( Figure 2E), and the posterior wall of the middle body of the stomach. In addition, the DBSS was used for full-thickness, simple, interrupted suturing with a 4-mm bite and a 4-mm pitch. Subsequently, 2 of the dogs were humanely killed and a pressure resistance

capacity of 1900 Pa(G) was confirmed by leak test ( Figure 2F). EFTR performed using only flexible endoscopy requires appropriate devices for obtaining the operative field and complete full-thickness suturing. In this study, we used animal models to show that EFTR can be performed safely in multiple locations within the stomach, and we believe that this technique can be applied clinically. ““Event Date and Venue Details from 2011 6th INTERNATIONAL SYMPOSIUM ON MOLECULAR INSECT SCIENCE 02–05 October Amsterdam, THE NETHERLANDS Info: www.molecularinsectscience.com 3rd INTERNATIONAL SYMPOSIUM ON ENVIRON-MENTAL WEEDS & INVASIVE PLANTS (Intractable Weeds and PlantInvaders) 02–07 October Ascona, SWITZERLAND C. Bohren ACW Changins, PO Box 1012, CH-1260 Nyon, SWITZERLAND Voice: 41-79-659-4704 E-mail: Christian.