I tried to get the spots better on the neons but I am not seeing them now under direct flashlight. Still worried about the spots though. These were bright white spots as if I took a pin with white paint on it and put it there.

If there are ich strands that are more heat resistant, I'd rather use medication than taking the risk of just using heat to cure ich. I lost 90% of my stock treating ich with just heat! It didn't get knocked out until I started medicating with Kordon Rid-Ich+

You have 4 fish in a 2.5 gallon? That's overstocked. I suggest only stocking 1 fish in the 2.5 gallon.

Sounds like there was something else wrong with them. You're experience is by far the exception.

Anyone who wants to know more about treating ich with heat can do a simple google search, and read the countless threads on dozens of forums on the matter. There are more successes than one can count.

Jim, if it looks like the fish has grains of salt on it, then it is ich. Anything else, is not ich, and raising the temp can be disastrous. If you can get a clearer picture, it would help immensely. In the first picture, I am pretty certain that there is no ich. It's clear enough to tell.

You have 4 fish in a 2.5 gallon? That's overstocked. I suggest only stocking 1 fish in the 2.5 gallon.

The 4 fish are the sick ones. I have a 50 gallon and a 2.5 gallon. The 4 in the 2.5 are the ones I worried were sick. I left the healthy ones in the 50 gallon.

They do not have anything that looks like ich type salt strands. I am not seeing the white spots at this point so I may put them back in the main tank. I tried shining a ultraviolet flashlight on them to see if it had the same properties as the actinic and did not see anything. Although the glo fish looked quite interesting!

Any thoughts on the discoloration on the danios? My reading on neon tetra disease showed that discoloration of the abdomen. Below is from fishchannel.com

At very low levels of infection there may be no symptoms visible at all, and it is usually the case that the more obvious symptoms of neon tetra disease are only apparent on heavily infected aquarium fish a few days away from death. Infected aquarium fish will often spend less time with their schoolmates than normal, typically hiding away under aquarium plants and showing no interest in fish food. The aquarium fish’s colors fade, and sometimes gray or white patches on the flanks become apparent. In advanced cases the aquarium fish may have trouble swimming, and the fish may develop odd swellings or contortions indicative of damage to the musculature. Usually the aquarium fish dies within two or three days of the first symptoms of neon tetra disease becoming apparent.

I kinda want to leave them in isolation for a week to see what happens.

In my experience, neon tetra disease kills pretty quickly once symptoms are exhibited. It's a terrible thing to have to deal with.

Just about all fish are susceptible to it - it is spread by consumption, whether a fish is eating an infected dead fish, or nipping an infected live fish. If you think it might be NTD, then separating the fish is the right thing to do.

Here are two videos. The first one is the ones I put into a quarantine. The second one is a neon in the main tank that is having that same color fading on his left flank as the danios. Turn your volume down, my kid wanted more cherios and he got his point across! At 13 seconds you can see the spot on the neon at its clearest. (video1)