Dear Netters,
I am in search of a monoclonal antibody that binds to the CH1 domain of
murine IgG1. I have searched Linscott's in vain. Although there are
many Mabs listed that bind IgG1, none have their binding sites mapped to
a particular domain. It is okay if there is cross reactivity to other
IgG isotypes. The important thing is that it binds to Fab fragments of
antibodies from A/J mice. (I don't know if there are any strain
specific allotypes in CH1.)
I have considered trying polyclonal reagents, such as those in isotyping
kits, but every polyclonal I have seen is from rabbit, goat, or sheep,
all of which result in cross-reactivity with other reagents I am using.
In particular, I'm having a devil of a time with sheep anti-M13, as it
seems to bind other bacterial antigens as well as M13 (it's
polyclonal).
I have had the best luck using mouse and rat monoclonals for my ELISAs
and would prefer to stay with them for now, as I haven't had any success
producing a monoclonal antibody against M13.
Thanks for any info you can send my way.
-Larry
lpcasson at phoenix.princeton.edu