Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LS-F11583 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat GSK3A / GSK3 Alpha in samples of Cell Culture Supernatants, Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of GSK3A / GSK3 Alpha as low as 0.312 nanograms per millilter.

Sample Type:

Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates

Format:

96-Well Strip Plate

Detection:

Colorimetric - 450nm (TMB)

Measurement:

Quantitative

Detection Range:

0.312 - 20 ng/ml

Sensitivity:

0.312

Precision:

Intra-Assay: CV<10% / Inter-Assay: CV<12%

Quality Assurance:

Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Kit Components:

Coated 96-well Strip Plate

Standard (Lyophilized)

Sample Diluent

Assay Diluent A

Assay Diluent B

Detection Reagent A

Detection Reagent B

Wash Buffer (25x concentrate)

TMB Substrate

Stop Solution

Adhesive Plate Sealers

Storage:

Short term: -20°C; Long term: -20°C.

Restrictions:

For research use only.

About GSK3A / GSK3 Alpha

Description: Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Requires primed phosphorylation of the majority of its substrates. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis.