Electro Cell Fusion Advantages v PEG

Features

Increased Reproducibility

To ensure accurate and reproducible cell fusion it is essential to align the target cells prior to the fusion event. If the ion concentration of the bufferis too high, ions will cause a water stream which will disturb the cell alignment (pearl chain). Uniquely, the LF101 enables the researcher tomeasure the resistance of the buffer prior to cell fusion and ensure the correct ion concentration for exact cell alignment (in all experiments). This greatly enhances reproducibility.

Large Choice of Electrode Configuration

A large selection of possible electrode configurations available. The electrodes have been specifically designed for the LF101 device.

Pulse Characteristics

Both automatic and manual pulse delivery (number of pulses 1-99) modes avaailable. Pulse interval can be set in increments from 0.1 - 9.9 seconds.

Voltage Settings

Voltage can be set in 1V resolutions. This is especially suitable for mammalian cloning. The device outputs a precise square waves which enhances cell fusion probability.

Ease of Operation

For ease of operation, each cell fusion parameter is displayed on its own individual LED.

DC Pulse Output

The LF101 enables the researcher to output the DC pulse either automatically or manually. The automatic output mode facilitates routinework. The manual output mode enables the researcher to apply a DCpulse while using a microscope - this is particularly useful when working with unfamiliar cells and during nuclear transplantation.

• Voltage
setting with 1V resolution is possible in the specialized setting for
mammalian cloning. It is suitable to output precisely the square pulse wave
shape and pulse range as preset and accordingly brings about a higher rate of
cell fusion probability.

• Specialized setting for plant
protoplast and mammalian cell fusion can provide a desirable condition for
the cell fusion, which is done with DC pulse (100V, Max.) of the precisely
controlled voltage and pulse range after producing the pearl chains with DC.
It can elevate the rate of hybridoma production (Cell fusion) probability.

• Post fusion function for damage recovery of the cell can
be operated with On/Off switching.

• Operation of the device is very easy because both of
automatic and manual (Number of pulses: 1-99) transmitting of the pulse are
possible. And the pulse interval can be selected to set between 0.1-9.9
seconds. The device can keep a constant operating condition anytime.

• A comprehensive selection electrodes
designed specifically for the device is available. Using the specifically designed electrodes
with the device facilitates very efficient gene transfection and cell fusion.