Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.

You can use these in your assignments if you so choose.

T/R lab

Red

Orange

Yellow

Green

Blue

Purple

Pink

And here's the gel with your recovered fragments/backbones, ready for ligation.

Row 1 legend:

Red backbone

Red fragment

1 kb ladder

Orange backbone

Orange fragment

Yellow backbone

Yellow fragment

1 kb ladder

Green backbone

Green fragment

Row 2 legend:

Blue backbone

Blue fragment

1 kb ladder

Pink backbone

Pink fragment

1 kb ladder

Purple backbone

W/F Lab

Red

Orange

Yellow

Green

Blue

Purple

Pink

And here's the gel with your recovered fragments/backbones, ready for ligation.

WF Legend:

Green backbone

Green fragment

1 kb ladder

Blue backbone

Blue fragment

Pink backbone

Pink fragment

1 kb ladder

Purple backbone

Purple fragment

You can upload the pre-purification gels for reference here

Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page!