From: Yoshiki Kawabe
Dear Bionetters!
I am about to start prepare nuclear extract from cultured cells.
And I'd like to check the ratio of broken cells by the staining of nucleus.
I have a paper in which auhtors stated the usage of Azure C for
the checking. However they didn't wrote in detail.
Is there anyone out there who has experience with these dye?
Please tell me how to use this dye (for example: concentration of
the stock and it's solvent).
I would really appreciate hear from you!
Sincerely yours,
Yoshiki Kawabe
(ykawabe at ddbj.nig.ac.jp)
University of Tokyo
JAPAN