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Fluorescence Attachment for Spectrophotometers

Publishing Venue

IBM

Related People

Hardy, WA: AUTHOR

Abstract

A commercially available double beam spectrometer is modified to increase its application to fluorescent analysis. Most double beam spectrophotometers that are primarily intended for absorption analysis employ a source of light, visible or infrared. The latter is divided into two beams by mirrors M(1) and M(2), each beam being chopped or modulated by rotating plates C, ultimately passing by means of a series of mirrors and lenses as two beams through a compartment D. Compartment D contains a standard absorbing medium and a sample holder for supporting a sample absorbing medium under tests. The two beams exit from the sample holder through openings O and O', such beams going to suitable analyzing equipment.

Country

United States

Language

English (United States)

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Fluorescence Attachment for Spectrophotometers

A commercially available double beam spectrometer is modified to increase
its application to fluorescent analysis. Most double beam spectrophotometers
that are primarily intended for absorption analysis employ a source of light,
visible or infrared. The latter is divided into two beams by mirrors M(1) and M(2),
each beam being chopped or modulated by rotating plates C, ultimately passing
by means of a series of mirrors and lenses as two beams through a compartment D. Compartment D contains a standard absorbing medium and a sample holder
for supporting a sample absorbing medium under tests. The two beams exit from
the sample holder through openings O and O', such beams going to suitable
analyzing equipment.

It is convenient to use this type of instrument as a monochromator, using the
electronics, light gathering systems, mirrors, etc., of the conventional double
beam spectrophotometer. The use of the device as a monochromator for
fluorescent analysis without the addition of auxiliary equipment is accomplished
in the following manner. The source is replaced by mirror M and the actual
source of light to be used for fluorescent analysis, which is a source of ultraviolet
rays, is placed within the sample compartment at location B. The sample being
analyzed is placed within the sample compartment D somewhere within the path
of light L. Mirror M is placed in the path of the ultraviolet light source B so that a
chopped beam...