Detecting sugars

I would like to look at various sugar levels e.g. G-1-P, R-1-P etc in
bacteria grown under a variety of conditions. Can anyone please
point me in the direction of the best way to isolate the sugar pool
and then to quantitate and identify, maybe mass spec but does someone
know a better way, maybe HPLC based?
Thanks in advance
Martin Cann
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Department of Biological Sciences
Durham University
South Road
Durham DH1 3LE
UNITED KINGDOM
Phone: (+44) 191 374 3353
Fax: (+44) 191 374 2417
e-mail: m.j.cann at durham.ac.uk
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