Non-Esterified Fatty Acid (NEFA)

HR Series NEFA-HR(2)

Intended Use

The Wako HR series NEFA-HR(2) is an in vitro enzymatic colorimetric method assay for the quantitative determination of non-esterified fatty acids (NEFA) in serum.

Method

Enzymatic

Special Features

Detects a variety of free fatty acids

Broad Linear Range (0.01- 4.00 mEq/L)

No extraction step required

Designed for automated analyzers

Measurements of fatty acids are used in the diagnosis and treatment
of various disorders of lipid metabolism. Labor intensive extraction/
titration methods have been commonly applied to the measurement
of free fatty acids in serum. These approaches are time consuming,
hazardous and not easily automated.
The Wako NEFA assay is an original test developed as an enzymatic
method which is available in a series of individual reagents. The need
for an extraction step has been eliminated. This enzymatic method
is accurate, precise, simple and fast. The Wako method relies upon
the acylation of coenzyme A (CoA) and ultimately results in a purple
colored product which can be measured colorimetrically at 550 nm.

Applications

Automated Analyzer Applications

To request an automated analyzer application, please email us at: Customer Service. Please include the names of the product and the analyzer/instrument.