ScaI

DNA restriction enzymes from Takara Bio are unsurpassed in quality and purity. We’ve been producing them for over 30 years and were the first manufacturers to offer commercially available restriction enzymes in Japan. Each lot of every DNA restriction enzyme undergoes stringent quality control tests. Takara Bio DNA restriction enzymes are supplied with optimized buffers that provide maximum activity during restriction enzyme digestion. Visit our Restriction Enzyme Applications pages for information on how to digest DNA, relative activity in each Universal Buffer, star activity, buffers for double digestion, effects of DNA methylation, how to inactivate enzymes, and more.

Features

DNA Restriction Enzyme: ScaI

Star activity: Unrelated sites may often be cut in the presence of Mn2+, and at alkaline pH or low ionic strength.

Notice: Do not dilute. This enzyme is unstable at low concentrations. Addition of BSA to a final concentration of 0.01% in reaction mixture increases the relative activity up to 120% at 30°C and up to 240% at 25°C, but does not increase at 37°C.