Cross-linking of CD300a on NK results in a down-regulation of their cytolytic activity, whilst cross-linking of CD300a on mast cells, inhibits IgE-induced degranulation and SCF-mediated survival. Furthermore, cross-linking of CD300a on the surface of eosinophils has been shown to significantly inhibit their survival, chemotaxis and activation in response to certain inflammatory cytokines, whilst eosinophil-derived MBP (major basic protein) and EDN (neurotoxin), can down-regulate CD300a expression on mast cells in vitro (http://www.ncbi.nlm.nih.gov/pubmed/16339535).

Intended Use

Target Species

Human

Product Form

Purified IgG - liquid

Reconstitution

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.

Preservative Stabilisers

0.09%

Sodium Azide (NaN3)

Immunogen

Human HPB acute lymphoid leukaemia cell line.

Purity

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Reagents In The Kit

Preparing The Antibody

Test Principle

Buffer Solution

Phosphate buffered saline

Storage

Store at +4oC or at -20oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name

Yes

No

Min Dilution

Max Dilution

Flow Cytometry

Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice

Recommended Protocol

Flow Cytometry

Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

ELISA

Immunohistology

Histology Positive Control Tissue

Immunofluorescence

Western Blotting

MCA2704GA detects a band of approximately 60kDa in HPB cell lysates under non-reducing conditions.