1a.Objectives (from AD-416)
The objective of this cooperative project is:.1)to determine the differences in the expression of genes and proteins in plants grown in a greenhouse environment (no UV-B) compared to those in 'field conditions' (normal ambient UV-B concentrations), and.2)to identify the protein and genetic changes during hidden hunger, and using this information, deveolop a technique that growers can use to test their plants for specific and/or general nutrient deficiencies.

1b.Approach (from AD-416)
Microarray techniques will be used to rapidly screen for global differences in gene expression between the two different UV-B environments and nutrient (i.e., N,P,K,Fe,B, and Mg) stress. The initial use of an existing model plant system (i.e., Arabidopsis thaliana) for this work will facilitate identification of specific genes that are responsive to the absence of UV-B during growth. After identifying such UV-B and nutrient stress responsive genes in Arabidopsis, we can then identify related genes in bedding plant species (i.e., impatiens, petunia, begonia, geranium, marigold, pansy, chrysanthemum, and New Guinea impatiens). Simultaneously, proteomics techniques (i.e., identification of proteins of interest by 2-D gel analysis, followed by protein sequencing) will be used to screen for global differences in protein expression between the two different UV-B environments.

3.Progress Report

Antibodies were generated to Boron transport protein, BOR 1, active transport membrane protein, from the previous specific cooperative agreement, has been characterized and used successfully in enzyme-linked immunosorbent assay (ELISA). A second antibody, Boron Nip5 is a facilitated-diffusion membrane protein. Both Boron antibodies will be necessary to differentiate among Boron deficiency, sufficiency, and toxicity. Similar work has progressed for iron metabolism and phosphorus transport protein. Testing of these inventions will begin at crucial intellectual property office at University of Toledo.
This research relates to ARS parent project Sub-objective 2a: evaluate the use of non-destructive sensor technology to measure and predict the impact of biotic and abiotic stresses in ornamental crops.
The project has been monitored by written reports, meetings and phone calls.