Summary

Secondary Antibodies

Negative Isotype Controls

Useful Reagents

Positive Controls

Histology Controls

More Images

References

Reviews

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Rabbit anti Human CD82 antibody recognises the carboxy-terminal region of human CD82, also known as KAI1, SAR2 and TSPAN27. CD82 is a ~30 kDa multi-pass transmembrane glycoprotein belonging to the tetraspanin (TM4SF) family.

CD82 acts as a cancer metastasis suppressor protein (Feng et al.2015), while ubiquitously expressed at moderate to high levels in many normal tissues, its expression is down regulated during tumour progression. Loss of CD82 and p53 is correlated with poor prognosis for patients with prostate and other forms of cancer (Liu et al. 2006).

Two isoforms of CD82 may be generated by alternative splicing. The c-terminal region is not affected and thus Rabbit anti Human CD82 antibody is expected to recognize both isoforms of human CD82 (UniProt: P27701).

Intended Use

Target Species

Human

Species Cross-Reactivity

Target Species

Cross Reactivity

Mouse

Rat

N.B. Antibody reactivity and working conditions may vary between species.

A peptide corresponding to a 15 amino acid sequence close to the carboxy terminus of Human CD82.

Purity

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Reagents In The Kit

Preparing The Antibody

Test Principle

Buffer Solution

Phosphate buffered saline

Storage

Store at +4oC or at -20oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name

Yes

No

Min Dilution

Max Dilution

Western Blotting

0.5

1.0ug/ml

Immunohistology - Paraffin(1)

2.5ug/ml

(1)

This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.