UNOsphere S cation exchange media are designed to meet the increasing needs of the biopharmaceutical industry for high-productivity chromatographic media. UNOsphere, a hydrophilic polymeric support based on a single-step polymerization process, delivers high productivity through high binding capacity and low backpressures. The single-step polymerization process produces a support unsurpassed in quality and batch-to-batch reproducibility.

UNOsphere S support is a strong cation exchanger.

Features include:

Optimized to operate under 2 bar backpressure at linear velocities of 1,200 cm/hr

Stable to 1 M NaOH exposure up to 10,000 hours

Efficient capture of biomolecules from crude feedstreams

Large pores that result in ultrahigh binding capacities at fast linear velocities

Hydrophilic polymeric beads

Robust polymer designed to withstand repeated clean-in-place cycles

Biopharmaceutical manufacturing quantities available

Fully supported for regulatory submission

Most production chromatography systems have maximum pressure limits of 3 bar. The median particle size of UNOsphere S media is 80 µm, which generates a backpressure of less than 2 bar at 1,200 cm/hr. The highly macroporous nature of UNOsphere S media provides high binding capacities of 60 mg human IgG per ml of media at 150 ml/min. Harsh clean-in-place and sanitization cycles will not affect the long-term stability of UNOsphere media.

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO 9001-registered corporation.

50–1,200 cm/hrUNOsphere S support packed into a 20 cm bed height and run at 1,200 cm/hr generates less than 2 bar backpressure.

Chemical stability

1.0 M NaOH (20°C)

Up to 2,000 hr

1.0 M HCl (20°C)

Up to 200 hr

pH stability range

pH 1–14

Regeneration conditions

1–2 M NaCl

Storage conditions

20% Ethanol 0.1 N NaOH for 30 days

What is the education discount policy?

For more than 15 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows high school and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices.

How do I apply for the education discount?

If you are an educator at the high school or college level, visit our Education Discount Policy page to establish an education account number.

If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the list price. If the problem persists, please call customer service at
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