EtBr ussage

I have used 1 ul of 10 ug/ul EtBr in 30 ml agarose for visualization
and it works great. In fact, I find that sometimes it works better. No,
you don't get more background because the EtBr runs backwards off the gel
and only the intercolated EtBr stays for visualization. We keep our gels
in a disposable plastic container and dry at low temp in an oven. They
then go through our hazardous waste system. I assume they are incinerated.
The running buffer could be treated of you are especially paranoid, but
the concentration of EtBr would be very low (max. 10ug/1.5L).
Sherry
SHERRY OLMSTEAD Title: Lab Technician
Forestry Canada Phone: (604) 363-0600
Victoria, B.C. Internet: SOLMSTEAD at A1.PFC.Forestry.CA