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High Throughput Gene Expression/Biomarker Core Laboratory

The High Throughput Gene Expression/Biomarker Core Laboratory run by Drs. Tanriverdi and Freedman provides High Throughput/Rapid Gene Expression and Complete miRNA profiling analysis by using gold standard qRT-PCR with the combination of custom Integrated Fluidic Circuit (IFC) technology from Fluidigm (South San Francisco, CA). Also, high-throughput SNP and multi-plate immunoassay services are available. The core lab is located in a modular class 10,000 clean room.

Technology

The qRT-PCR analyses are performed on IFC chips (Dynamic Arrays 1) using a Liquid Handling Robot (Janus Automated Workstation2). Dynamic Arrays are able to conduct 9,216 Real-Time PCR reactions extremely efficiently and rapidly (within 3 hours). The primary advantage of this platform and technology is that it can complete thousands of reactions with minimal pipetting steps and much smaller volumes of reagents. This significantly saves time and experimental costs.

References/Links

Director's Message

The High Throughput Gene Expression/Biomarker Core Laboratory provides rapid, cost-effective gene expression, miRNA and biomarker analyses on the UMMS campus. Our new facility provides a wide range of experimental capabilities through fee-for-service, educational and collaborative relationships in the areas of gene expression, miRNA and clinical biomarker research. We provide a flexible platform with rapid service and the highest quality control to meet the needs of basic scientists or large clinical studies. We work internal investigators, external academic researchers and industrial partners.