Executive Summary: LESA and LESAplus liquid chromatography provide novel surface analysis tools for the spatial resolution of analytes from a wide variety of surfaces. Whole body or organ sections in particular can be probed for small molecules, lipids and proteins with a resolution of 400 – 1000μm. Commercial solution allows for automated processing of samples (Advion TriVersa NanoMate®…

The TriVersa NanoMate® LESA® is the latest in chip-based electrospray ionization technology from Advion. It combines the benefits of liquid chromatography, mass spectrometry, chip-based infusion, fraction collection and direct surface analysis into one integrated ion source platform. It allows scientists to obtain more information from complex samples than LC/MS alone.

Infusion based lipidomics analysis (shotgun Lipidomics) is an analysis strategy that involves the liquid extraction of lipids from samples such as blood, tissue homogenates or dried blood spots. This approach is suitable for a wide range of biomedical questions of lipid differentiation and lipid flux in the field of metabolic disease, neurological disorders, cancers or eye…

Liquid Extraction Surface Analysis Mass Spectrometry (LESA-MS) has been utilized for determination of small molecules, lipids and proteins from a variety of surfaces such as tissue sections, plant material, medical devices, TLC plates or DBS cards. It allows direct surface analysis without sample preparation from spot sizes of 1 mm diameter by aspiration of extraction…

The new Liquid Extraction Surface Analysis (LESA) mode of the TriVersa NanoMate can now be combined with nano liquid chromatography and a smaller extraction drop size of 400 µm to result in a powerful novel surface analysis mode. Resulting in the detection of small molecule drugs from tissue sections, residue on plant material or lipid…

Residues and contaminants in the food chain are an increasing problem due to high production volumes, large area distribution and import/export of food items across the world. The novel LESAPLUS surface analysis approach combines the standard liquid extraction surface analysis with an additional step of a nano liquid chromatography separation. This combination is ideally suited…

Spatial lipid composition, distribution and regulation are very important factors for mediating lipid functionality and, when disrupted, can cause pathophysiological processes leading to cancer, obesity, atherosclerosis, and neurodegeneration. The novel LESAPLUS surface analysis approach combines the standard liquid extraction surface analysis with an additional step of a nano liquid chromatography separation. This combination is ideally…

One of the benefits of nanoelecrtospray ionization (nanoESI) is the reported optimal ionization efficiency (equalized response) of chemically different analyses despite the presence of matrix components. However, outside of a very low flow rate of ca. 1-20 nL/min, the ionization efficiency drops exponentially and coupling higher flow rates from a typical liquid chromatography (LC) system…

ChipSoftX is an entirely new operating software for the TriVersa NanoMate automated nanoelectrospray source. Besides improvement in program compatibility with Windows and integration of existing software features, it also provides access to the new Developers Kit – a platform for customized method development with direct access to robot controls allowing entirely novel analysis workflows such…

Surface analysis techniques have gained significant attention over the last couple of years with LESA being an interesting alternative to techniques such as SIMS or MALDI imaging. This note demonstrates practical applications for LESA in the material sciences.

More analytes and higher sensitivity Developed in collaboration with Oak Ridge National Laboratory*, the liquid extraction surface analysis (LESA) capability of the TriVersa Nanomate® enables simple, direct ESI mass spectrometric analysis from a variety of surfaces.

Automated static nano-ESI/MS/MS (Infusion MS/MS) is an easy-to-use and high-throughput approach to analyte identification. It is particularly useful in the field of shotgun lipidomics, which itself has great potential for biomarker discovery and gained some significant attention in the last couple of years (Schuhmann et al. 2012; Jung et al. 2011; Han et al. 2011).…

In many cases of routine protein identification, processing times become the major bottleneck at the core facility. Although a nano-LC/MS/MS approach is recognized as a general strategy for protein identification, the typical 30-120 min run-time prohibits fast turnaround times. The TriVersa NanoMate offers a more rapid approach for the infusion of the protein digest, with…

Presented by: Dr. Tom Covey In this web presentation Dr. Tom Covey describes how researchers can incorporate the fast, simple & direct method of LESA Clarity for AB SCIEX Mass Spec Systems into their labs. He shows data that illustrates how you can see more analytes with better sensitivity.

Presented by: John P. Shockcor, Director of Life Sciences Business Development, Waters Corp., Visiting Fellow, Dept. of Biochemistry, University of Cambridge, UK Description: Profiling low level components in a complex mixture of small molecules can be a challenging task. Although it may be possible to detect many low level components in a complex mixture, characterization…

Presented by: Thomas Covey, Principal Research Scientist at AB SCIEX Thomas Covey describes how various organizations are incorporating the fast, simple, and direct method of LESA – Liquid Extraction Surface Analysis – into their labs. He shows data that illustrates how you can see more analytes with better sensitivity by combining LESA with mass spectrometry…

In this webinar, Dr. Han describes how he uses the TriVersa NanoMate’s chip-based nanoelectrospray ionization capabilities in infusion mode to obtain more information from complex samples and faster lipids analysis with no sample-to-sample carryover. The interests of Dr. Han’s laboratory have been focused on the altered lipid metabolism, trafficking, and homeostasis under patho(physio)logical conditions. Currently,…

Presented by: Gavin Reid, Associate Professor at Michigan State University A large number of studies have demonstrated that disruption of lipid metabolism or signaling pathways can play a key role in the onset and progression of human disease, including cancer and diabetes. Thus, a comparative analysis of changes in individual lipids or lipid profiles (i.e.,…

Negative ion nanoelectrospray ionization mass spectrometry (nESI-MS) is notoriously challenging, even more so under the high aqueous conditions required for protein-ligand interactions at physiological conditions. However, we present a successful application of negative ion nESI-MS for the investigation of noncovalent interactions between Ψ-RNA and small molecule inhibitors.