Thank you very much for your interest in ab2318 Anti-Prostaglandin E2 antibody.

To our knowledge, ab2318 has still not been tested in a cell culture assay due to the sodium azide. As my collegue suggest it is possible to extract the chemical via a dialysis step to purify the antibody or to use our purification kit, whichwouldpurify this whole serum antibody on top of this. The purification kit can be found here:

https://www.abcam.com/index.html?datasheet=109208 (or use the following: https://www.abcam.com/index.html?datasheet=109208).

As the application hasn't been tested, I can offer a discount off a future purchase if you buy ab2318 now, test it in cell culture assay and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab2318 in cell culture assay. I will then send a discount code. This code must be issued before purchasing ab2318 so please wait for my reply before ordering.

2. Purchase ab2318 either by phone, fax, or online (www.abcam.com).

3. Test it in cell culture assay.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab2318 turns out to be unsuitable for cell culture assay, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

I am very pleased to hear you would like to accept our offer and test ab2318 in IP. This code will give you 100% off your next order before the expiration date. To redeem this offer, please submit an Abreview for IP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

I have to confirm thatab2318 is not eligible for the testing programmewith different species.

The target Prostaglandin E2 is not a protein but a lipid. This chemical will be the same independently of the species.

For more information on the structure please follow this link:

http://en.wikipedia.org/wiki/Prostaglandin_E2

Your customer should not have any problems detecting Prostaglandin E2 in rabbit samples.

Please keep in mind that since PGE2 is a lipid, extraction buffers containing detergents (for preparing tissue samples) are not recommended. Mechanical disruption in a buffer such as PBS is recommended instead. For serum and plasma, simple dilution should be sufficient.

I hope this information is helpful and wish your customers good luck for their experiments.

I performed a protein alignment with the target immunogens and the rabbit protein sequences for the antibodies. We recommend that alignment should be over 85% to predict that an antibody will detect in a different species.

Here are the results, similarities are in %:

ab47027 Anti-CGRP antibody: 85%

ab14184 Anti-Substance P antibody [SP-DE4-21]: 100%

ab106291 Anti-Substance P antibody: 100%

ab2318: As mentioned in the Scientific Support section, PGE2 is a lipid rather than a protein and is therefore not species specific. There should not be any particular problems using this assay to detect PGE2 from rabbit. Optimization for different sample types will have to be done by the end user. Please keep in mind that since PGE2 is a lipid, extraction buffers containing detergents (for preparing tissue samples) are not recommended. Mechanical disruption in a buffer such as PBS is recommended instead. For serum and plasma, simple dilution should be sufficient.

I can offer a discount off a future purchase if your customer buys one or more of the antibodiesnow, test it in rabbit and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback.