Amino Linker Phosphoramidites

Amino linker phosphoramidites for the functionalization of the 5'-end during oligo synthesis Features • Amino linker products are coupled with standard synthesis protocols, identical to the coupling of DNA monomer phosphoramidites. • 5'-Amino phosphoramidite is used to add a functional amine group at the 5'- or 3'- end of the oligonucleotides. The primary amino group can be used for subsequen...read more

Description

Amino linker phosphoramidites for the functionalization of the 5'-end during oligo synthesisFeatures

• Amino linker products are coupled with standard synthesis protocols, identical to the coupling of DNA monomer phosphoramidites.• 5'-Amino phosphoramidite is used to add a functional amine group at the 5'- or 3'- end of the oligonucleotides. The primary amino group can be used for subsequent conjugation of a variety of reporter dyes or labels.• The base-labile TFA-group is easily cleaved with concentrated ammonia during the cleavage and deprotection steps. Additional deprotection steps are not necessary.• The DMT-group can be cleaved more conveniently than the MMT-group on the synthesis instrument with acidic deblocking solution to enable on-support labeling protocols.• 5'-DMT Amino linker can be treated by normal deprotection and cleavage.