Electrophoretic study on the water soluble proteins of dog salmon eggs

Yamamoto, T. S., 1976: Electrophoretic study on the water soluble proteins of dog salmon eggs. Journal of the Faculty of Science Hokkaido University Series VI Zoology 20(3): 245-252

The water soluble proteins of the eggs of the dog salmon, Oncorhynchus keta, were separated by electrophoresis on cellulose acetate strips and 7 bands were detected. Up to 12 h after fertilization or parthenogenetic activation of the eggs, no change in the electrophoretic pattern was detected. The slow-moving band consisted of carbohydrate-protein complexes, the mobility of which did not alter after treatment of the eggs with isotonic CaCl2 solution. This band may represent the contents of cortical alveoli. The fastest-moving band was also composed of carbohydrate-protein complexes. Its mobility considerably decreased after treatment of the eggs with CaCl2 solution. This band may represent yolk protein. The mechanism underlying the inhibition of the breakdown of cortical alveoli in the eggs immersed in isotonic CaCl2 solution is discussed in relation to the electrophoretic property of the alveolar contents.