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疾病相关A chromosomal aberration involving TGFB2 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with HDAC9.Loeys-Dietz syndrome 4Defects in TGFB2 may be a cause of non-syndromic aortic disease (NSAD). NSAD is a frequently asymptomatic but potentially lethal disease characterized by thoracic aortic aneurysms and dissections without additional syndromic features.

序列相似性Belongs to the TGF-beta family.

翻译后修饰The precursor is cleaved into mature TGF-beta-2 and LAP, which remains non-covalently linked to mature TGF-beta-2 rendering it inactive.

Anti-TGF beta 2 antibody 图像

ab36495 at 1/1000 staining mouse kidney tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step was perfomred in citrate buffer. The tissue was then blocked and incubated with the antibody for 1 hour. A biotinylated rabbit anti-mouse antibody was used as the secondary.

Cells were fixed with formaldehyde, permeabilized with 0.1% Tween/TBS and blocked with 10% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/50 in 10% BSA/TBST) for 1 hour. An AlexaFluor®488-conjugated goat anti-mouse monoclonal IgG (1/250) was used as the secondary antibody.

TGF beta 1 is often observed at about 12 kDa on blots, since the 48 kDa precursor is processed to a 12 kDa. I suspect that the band you observe between the two lower molecular weight markers is the 12 kDa subunit...

This antibody was tested in WB with recombinant TGFb2 which therefore can be used as positive control. We also have a positive Abreview from a customer which used mouse kidney and had veryclear positive staining. Read More

ab9758 - the major band at44.3 kDa corresponding to the unprocessed (pre-pro-TGF beta 1) form, the mature processed TGF beta 1 is a 25 kD homodimer composed of two 12.5 kDa subunits joined by disulfide bonds. As th...

We usually present the MSDS documents to the right hand side of the datasheet of products. The product you are interested in (ab36495) does not have an MSDS presented as it does not contain any materials whi...

Thank you for your enquiry. Yes, you are correct that ab36495, although it is a mouse antibody, can recognize mouse TGF beta-2. Here is a review from a customer who tested ab36495 for reactivity to mouse tissue: http://www.abcam.com/index.html?dat...

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