Product Category

Biosafety Level

Dissociation Reagents

Storage:
When stored at 2°C to 8°C, the product is stable until the expiration date on the label.

Applications:

Trypsin is the most commonly used enzyme for harvesting cells in culture. A non-enzymatic approach is needed when non-protein and animal-component free materials are required. ATCC Non-Enzymatic Cell Dissociation Solution is a sterile, phenol-red free solution composed of a proprietary mixture of chelators representing an optimized alternative to protein-digesting enzymes.

0.25% Trypsin/0.53 mM EDTA in Hanks Balanced Salt Solution without calcium or magnesium. For dissociation of cell monolayers. Trypsin-EDTA solution is suitable for most but not for all adherent cell lines. For cell line-specific information, please go to the appropriate product page on the web, refer to the product sheet supplied with the cell line, or contact ATCC Technical Service.

NOTE: Each cell line responds to ATCC® Trypsin-EDTA solution in a unique manner. For optimum results, observe the cells during the dissociation process to avoid over-trypsinization. For more information, please refer to the General Protocol for Using ATCC® Trypsin-EDTA Solution. For cell line-specific information, please refer to the product sheet supplied with the cell line, or contact ATCC® Technical Service. ATCC® Trypsin-EDTA solution is suitable for most but not for all adherent cell lines. This product contains phenol red.