[Histonet] FISH fading

From:

"Bonnie Whitaker"

Hi Histonet!
A colleague called to vent some frustrations about FISH today. This person
is performing FISH, checking the slides, and thinking the results are good.
A few days later when the PI checks the slides, the slides have faded. Is
there a specific rule of thumb for how much exposure to light (sunlight and
artificial) and how much time can pass without damaging the signal? Are
anti-fading agents different? Should he try something other than what the
manufacturer includes in the kit that they are using?
Bonnie Whitaker
Lab Manager
Brown & Associates Medical Laboratories
8076 El Rio
Houston, Texas 77054
713-741-6677
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