Dear Netters,
I am looking for a HIS4 plasmid to use as a prototrophic marker and for
gene disruption in our his4-580 strains. I believe the standard vector is
pPB54 containing a 1.8 kb SacI-BstEII restriction fragment, but any
vector with the marker would be appreciated.
If anyone can supply such a vector, please let me know.
Thanks.
Pat Burke <burkep at spot. Colorado. EDU>
MCD Biology
University of Colorado
Boulder, CO 80309