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Event description

Description

For Lonza Employees Only

Acquity UPLC H-Class Bio for lab practitioners

Instruction for the ACQUITY UPLC H-Class Bio is a two day course designed to offer a solid understanding of ACQUITY UPLC H-Class Chemistry, Instrumentation and Software. Instrument overview, chromatographic concepts as well as Method Transfer and Development will be taught.

Topics Covered

UPLC theory & chemistry

UPLC principle & theory

Resolution & peak capacity

UPLC column chemistries

Column design

System bandspreading

ACQUITY hardware

Solvent manager

Degasser

Sample manager

Column manager

eCord technology

Detectors

ACQUITY console software

Overview of console software

Module functions and diagnostics

Interactive displays

Plots and logs

Preparing for analysis

Characterizing needle and loop volume

Instrument method parameters

Method transfer, HPLC to UPLC

Method transfer considerations

Transfer of isocratic methods

Transfer of gradient methods

System delay volume

Using the ACQUITY column calculator

Troubleshooting tips and tricks

Mobile phase preparation

Controlling bacterial growth

Column maintenance

Tips and tricks for the solvent and sample manager

Optimal detector settings

Troubleshooting peak shape problems and carry over

Hands-on exercises

Who Should Attend

The course is designed for users who currently use the ACQUITY UPLC H-Class Bio System. For the user who is involved in Instrument Operation and for those whose responsibilities include Method Transfer and/or Method Development