Tuesday, December 8, 2009

New Human Natural Killer Cell purification kit

We have developed two new kits for the isolation of untouched Natural Killer cells from both mouse and human preparations that achieves levels of purity as high as 95%. Undesired populations are negatively depleted using a cocktail of monoclonal antibodies that specifically react with non-NK cell populations. These cell types are then tagged with magnetic beads and separated from the desired NK cells by magnetic force. A typical isolation is achieved in 45 minutes.

Two examples of enrichment of NK cells from human PBMC using MagCellect NKcell enrichment kit (R&D Systems MAGH109). Cells before and after enrichmentwere double-stained with APC-conjugated anti human CD3 (R&D Systems FAB100A) and PE-conjugated anti human CD56 (R&D Systems FAB2408) to specifically label the NK cell populations. Panel A, Isolation of NK cells from a PBMC prep consisting of mostly CD56dim cells. Panel B, enrichment of both CD56dim and CD56bright from a different PBMC preparation; CD56dim NK cells are shown in green and CD56bright NK cells are shown in blue.

Phenotypic characterization of MagCellect-isolated NK cells

We have used the most recent and established markers and techniques to extensively characterize the isolated NK cells. We show here that the highly pure NK cell populations (both mouse and human) express NK cell-specific markers (i.e., NKp46, Nkp80, NKp30, CD56, NKG2D, KIR3DL1 and NTB-A)

List of R&D Systems APC- or PE-conjugated antibodies used in the phenotypic characterization of isolated NK cells:

We tested the efficacy of our new MagCellect NK cell kits by comparison to other systems in the market, with typically better or similar results. But unlike other commercially available kits that require a costly set of magnets and supplies (expensive magnets and columns), our kits were developed to work with different kind of commercially available magnets and systems, providing more flexibility, simplicity and cost-efficacy.We realize that some people already invested large amounts of money in costly magnets and supplies from our competitors, so we developed our MagCellect kits to be able to work with optimal performance using different type of magnets and supplies.

To see how our kits compare to other commercially available isolations systems, and how you can effectively use MagCellect NK Isolation kits with other suppliers magnets and/or column, check out the data presented here.