Cell Proliferation Assay Is Performed by Creative Proteomics

January 3013:572018

As an experimental technique, cell proliferation detection is not only very important in the study of the basic biological characteristics of cells, but also a basic method for analyzing cell state, studying genetic traits and assessing stress responses. Creative Proteomics’ cell proliferation assays provides a highly sensitive and economical detection service. The main methods of cell proliferation detection include MTS, MTT, BrdU, DNA Synthesis, etc.

EdU is a thymidine analogue that penetrates into the replicating DNA molecule at the time of cell proliferation instead of thymidine, to detect the cell DNA replication activity, through the specific reaction of EdU and Apollo® fluorescent dyes. It is suitable for cell proliferation, cell differentiation, DNA repair, virus replication, cell marker tracer and so on, especially for screening of siRNA, miRNA, small molecular compounds and other drugs.

MTT is also known as MTT assay which is a method for detecting cell survival and growth. The mechanism is that succinate dehydrogenase in mitochondria of living cells can return exogenous MTT to water-insoluble blue-purple crystal Formazan and make it deposit in cells, but dead cells have no such function. Dimethyl Sulfoxide (DMSO) can dissolve the metasan in the cells. The light absorption value of DMSO can be measured at wavelength 490 nm by enzyme linked immunosorbent assay (Elisa), which can indirectly reflect the number of living cells. The amount of MTT crystal formation was proportional to the number of cells in a certain range of cells. This method has been widely used in the detection of bioactive factors, large-scale screening of anti-tumor drugs, cytotoxicity test and radiosensitivity test of tumor.

MTS is biologically returned by cells to a shabby compound that is soluble in tissue culture. Dehydrogenase classes in living cells, which are metabolically active, convert MTS into a liquid soluble methamphetamine compound. The absorption value of the compound at 490 nm can be measured directly on the 96 hole plate without additional treatment. MTS assay is more sensitive than other methods such as MTT, XTT and WST-1 in determining the number of living cells in cell proliferation or toxicity test.

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