Reviews

-

Multiple isoforms of coronin-1c may be generated by alternative splicing. All described isoforms contain the immunogen sequence used to generate Mouse anti Human CORO1C antibody, clone 1F7 which is expected to recognize all described isoforms of human CORO1C.

Intended Use

Target Species

Human

Product Form

Purified IgG - liquid

Reconstitution

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Preservative Stabilisers

None present

Immunogen

Recombinant protein corresponding to aa 375-474 of human CORO1C

Purity

Approx. Protein Concentrations

Ig concentration 0.5 mg/ml

Reagents In The Kit

Preparing The Antibody

Test Principle

Buffer Solution

Phosphate buffered saline

Fusion Partners

Spleen cells from immunized Balb/c mice were fused with cells from the Sp2/0 myeloma cell line.

Storage

Store at -20oC only.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name

Yes

No

Min Dilution

Max Dilution

Western Blotting

0.1

10 ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.