Product Description: Isolation of high-quality plasmid DNA from the gemisch of the E. coli cell lysate paves the way for many laboratory as well as clinical genetic applications, among which are clone screening, DNA sequencing, DNA transfection, probe preparation, PCR analysis, and gene therapy, to name but a few. Although small-scale plasmid purification (mini-prep) is often sufficient for DNA cloning and sequencing, large-scale (maxi-prep) plasmid purification is required for gene transfer and gene therapy studies. Our Maxi-Plasmid Isolation Kit is similar to the Mini-Plasmid Isolation Kit with additional filtration and precipitation steps. Plasmid DNA can be easily and rapidly purified without the use of phenol. Each Maxi-prep DNA is purified from 100 - 200 ml of overnight E. coli culture in 90 min with a typical yield of 0.5 – 3 ug DNA (as determined by plasmid replication origin) per ml of culture. Purified plasmid DNA, typically with an A260/A280 ratio of ~2, is suitable for restriction digestion, probe preparation, DNA sequencing, PCR analysis, and transfection. The kit (sufficient for 10 maxi-preps) is stable for at least one year if handled and stored properly.