The secreted alkaline phosphatase (SEAP) is widely used as reporter gene to analyze the activity of promoters and transcriptional factors and to tract gene expression in cell culture or animals. SEAP can be effectively secreted into culture medium and serum. This unique characteristic of SEAP makes it convenient to kinetically analyze gene expression over a period of time using a single culture or animal. SensoLyte® FDP secreted alkaline phosphatase reporter gene assay kit provides a sensitive fluorimetric assay of placental alkaline phosphatase for both secreted and membrane-bound form of placental alkaline phosphatases. The kit contains: FDP fluorogenic substrate (Ex/Em=485±20/528±20 nm upon dephosphorylation), Assay buffer, Stop solution, A “mix and read” assay protocol that is compatible with high throughput screening liquid handling instruments