Note

DNA vectors used in this cell line modification service will NOT be transferred to clients under this arrangement.

Note

Price/availability/specifications subject to change without notice.
Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.

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Knock-out and knock-in cell lines are generated by experienced scientists working on lentivirus, adeno-associated virus (AAV), RNAi, and cell line development services for decades. In combination with the powerful CRISPR/Cas9 tool, our proprietary viral system is highly efficient and precise in knock-out and knock-in cell line development with any gene and any host cell, including mammalian primary cell, without introducing foreign DNA.

CRISPR/Cas9 DNA constructs are introduced into host cells by viral transduction or non-viral transfection. Knock-out and knock-in cell lines are screened for permanent and precise modification of cellular genome. Our fast and predictive screening techniques may significantly increase speed of knock-out and knock-in cell line development while improving quality and uniformity. The process includes:

- Molecular construction of gRNA, Cas9, and donor vectors based on the target gene/site/locus and cell line specified by clients;
- Virus packaging and production;
- Viral transduction or non-viral transfection of DNA constructs into host cells;
- Selection of cell pool or screening for stable single clones;
- Validation of cell pool or single clones by sequencing and PCR or specific methods such as FACS; (Note: The standard method for validation is sequencing and PCR. Additional fee will be charged for Western blot or cell-based assay of your interest.)
- Batch culture and cell banking.

Service of knock-out cell line generation using CRISPR genome editing includes: