2020-06-07T08:49:59Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/495762018-08-07T07:36:07Zcom_10261_44755com_10261_1col_10261_44757Different steps of sexual development are differentially regulated by the Sec8p and Exo70p exocyst subunitsSharifmoghadam, M. R.León, Nagore deHoya, MartaCurto, María ÁngelesValdivieso, María HenarYeastSexual developmentMatingSporulationExocystProtein trafficking10 páginas, 5 figuras.-- El pdf del artículo es la versión pre-print.In this paper we show that in Schizosaccharomyces pombe, mating-specific cell adhesion is dependent on the exocyst subunit Sec8p, but independent of the exocyst subunit Exo70p. In the absence of Exo70p, the forespore membrane does not develop properly and the leading edge protein Meu14p is abnormally distributed. Additionally, the spindle pole body is aberrant in a significant number of exo70Δ asci. In both the sec8-1 and the exo70Δ mutants, the development of the spore cell wall is impaired. These results show that different steps of sexual development are differentially regulated by the exocyst and suggest the existence of exocyst subcomplexes with distinct roles in mating.This work has been supported by grants
BFU2007-61866 from the CICYTand GR231 from the Junta
de Castilla y León, Spain. M.R.S. and N.d.L. were supported
by fellowships from the Iranian and Spanish Ministry of
Science, respectively.Peer reviewed2012-05-11T13:13:50Z2012-05-11T13:13:50Z2010-04artículoFEMS Microbiology Letters 305(1): 71-80 (2010)0378-1097http://hdl.handle.net/10261/4957610.1111/j.1574-6968.2010.01915.x1574-696820180855enghttp://dx.doi.org/10.1111/j.1574-6968.2010.01915.xopenAccessBlackwell Publishing