Contributions

An autonomous control framework for cell out-of-plane reorientation has been proposed by utilizing a robot-tweezers cell manipulation system. A serious of experiments that include cell in-plane, out-of-plane rotational control and cellular orientation-based enucleation manipulation have been performed, demonstrating the necessity and significance of cell orientation control involved in many cell surgery studies. The outcome of this study represents an advancement in single cell manipulation, and contributes to many cellular orientation-based cell surgery applications, such as cell nucleus biopsy and transplantation, cloning technology, and PGD.