{"files"=>["https://ndownloader.figshare.com/files/816068"], "description"=>"<p>Before each test, the piston spirometer was purged and partially filled with 5 liters of clean dry air. When the patient coughed into the system mouthpiece, the cough flowed through an ultrasonic spirometer which measured the cough volume and flow rate. The cough then flowed through a valve and into the piston spirometer, displacing the piston to the right. When the subject finished coughing, the valve was closed and the aerosol sampler was turned on. The cough aerosol was pulled out of the spirometer and collected by the aerosol sampler. As the aerosol sampler drew air, the piston moved to the left until no air remained in the spirometer.</p>", "links"=>[], "tags"=>["aerosol"], "article_id"=>486431, "categories"=>["Biotechnology", "Medicine", "Infectious Diseases"], "users"=>["William G. Lindsley", "Francoise M. Blachere", "Robert E. Thewlis", "Abhishek Vishnu", "Kristina A. Davis", "Gang Cao", "Jan E. Palmer", "Karen E. Clark", "Melanie A. Fisher", "Rashida Khakoo", "Donald H. Beezhold"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0015100.g003", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cough_aerosol_particle_collection_system_/486431", "title"=>"Cough aerosol particle collection system.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2010-11-30 01:47:11"}

{"files"=>["https://ndownloader.figshare.com/files/407057"], "description"=>"<div><p>Influenza is thought to be communicated from person to person by multiple pathways. However, the relative importance of different routes of influenza transmission is unclear. To better understand the potential for the airborne spread of influenza, we measured the amount and size of aerosol particles containing influenza virus that were produced by coughing. Subjects were recruited from patients presenting at a student health clinic with influenza-like symptoms. Nasopharyngeal swabs were collected from the volunteers and they were asked to cough three times into a spirometer. After each cough, the cough-generated aerosol was collected using a NIOSH two-stage bioaerosol cyclone sampler or an SKC BioSampler. The amount of influenza viral RNA contained in the samplers was analyzed using quantitative real-time reverse-transcription PCR (qPCR) targeting the matrix gene M1. For half of the subjects, viral plaque assays were performed on the nasopharyngeal swabs and cough aerosol samples to determine if viable virus was present. Fifty-eight subjects were tested, of whom 47 were positive for influenza virus by qPCR. Influenza viral RNA was detected in coughs from 38 of these subjects (81%). Thirty-five percent of the influenza RNA was contained in particles >4 µm in aerodynamic diameter, while 23% was in particles 1 to 4 µm and 42% in particles <1 µm. Viable influenza virus was detected in the cough aerosols from 2 of 21 subjects with influenza. These results show that coughing by influenza patients emits aerosol particles containing influenza virus and that much of the viral RNA is contained within particles in the respirable size range. The results support the idea that the airborne route may be a pathway for influenza transmission, especially in the immediate vicinity of an influenza patient. Further research is needed on the viability of airborne influenza viruses and the risk of transmission.</p> </div>", "links"=>[], "tags"=>["measurements", "airborne", "influenza", "aerosol", "particles", "coughs"], "article_id"=>140413, "categories"=>["Medicine", "Cancer", "Biotechnology"], "users"=>["William G. Lindsley", "Francoise M. Blachere", "Robert E. Thewlis", "Abhishek Vishnu", "Kristina A. Davis", "Gang Cao", "Jan E. Palmer", "Karen E. Clark", "Melanie A. Fisher", "Rashida Khakoo", "Donald H. Beezhold"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0015100", "stats"=>{"downloads"=>10, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Measurements_of_Airborne_Influenza_Virus_in_Aerosol_Particles_from_Human_Coughs/140413", "title"=>"Measurements of Airborne Influenza Virus in Aerosol Particles from Human Coughs", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2010-11-30 00:06:53"}