RNA was extracted from bud using a using EasySpin RNA Reagent. The RNA concentration and integrity were assessed using Nanodrop and Agilent 2100. The library was generated using the NEBNext® UltraTM RNA Library Prep Kit for Illumina®. The amplified fragments were sequenced using the Illumina HiSeq X-10 platform and 150 bp paired-end reads were obtained by Biomarker Technologies Co?LTD