Interacts with 23S, aiding in the binding of the two ribosomal
subunits (50S+30S).

Stabilizes correct codon-anticodon pairing in the A site, via a
hydrogen bond formation between the N1 atom of Adenine (see image of Purine chemical
structure) residues 1492 and 1943 and the 2'OH group of
the mRNA backbone.

Universal
Primers

The 16srRNA gene is used for phylogenetic studies[3] as it
is highly conserved between different species of bacteria and
archaea.[4]
In addition to these, mitochondrial and chloroplastic rRNA are also
amplified.

Universal (or quasi-universal as it does not pick up some
recently discovered hydrothermalarchaea species belonging to the phylum Nanoarchaeota[5])PCRprimers are used to amplify
the 16srRNA gene to provide phylogenetic information.

Comparative analysis of the 16S rRNA sequences is done with the
help of primers, called "universal primers" which have the
sequences: