Effect of some nutrients on the growth of 3 yeast strains in the pulp mill waste liquor was determined during an attempt to lower the BOD content of the waste liquor and to produce the fodder yeast. The strains applied were Debaryomyces castelli Capriotti, D.phoffi Capriotti, and Cryptococcus luteolus (Saito)Skinner. The necessity of the addition of 0.2% 0.5% yeast extract, 0.2% , and 0.1% .O for the best growth of all three strains in the waste liquor was ascertained as a result. After 3-day treatment of the yeast cells on the waste liquor, the BOD content was lowered by about 60-70%. Harvested yeast cells contained ca. 75% water with 1.5-3% lipid, 40-46% protein, 50% carbohydrate and 3-5% ash on the dry weight basis, indicating the possibility of being utilized as the fodder yeast.

About 600 strains of bacteria which can utilize methanol as the sole source of carbon were isolated from natrual sources, and their ability to produce amino acids on the medium containing methanol as the sole source of carbon was tested by paper chromatography, by which 3 strains were screened by virtue of their relatively shperior amino acid production. These were tentatively identified as follows ; one strain belonged to the genus methylomonas, while the remaining two were members of the genus Hyphomicrobium. Further studies on their growth characteristics were carried out with relation to amino acid production. According to these experiments, the following results were obtained. 1) No vitamin added was effective for the growth enhancement. 2) Optimum initial concnetration of methanol was proved to be one per cent (v/v), and no conspicuous effect of feeding methanol on the growth of these bacteria ould be observed, provide that the initial methanol concentration was controlled to 1 per cent. 3) Among the nitrogen sources tested, 0.1 per cent of enabled the best growth. 4) Addition of 0.001 per cent largely increased the growth of all three strains. Other metal ions tested were either not effective or strongly inhibitory for the growth. 5) Addition of 0.1 per cent each of yeast extract, corn steep liquor, beef extract, and peptone also increased the growth markedly. 6) About g/ml of alanine could be obtained finally after 64 hr culture of Hypm. A on the medium lcontaining 0.1 per cent yeast extract and 1 percent methanol. Approximately g/ml of glutamic acid by Hypm. GA and g/ml of alanine by Mtlm. A were produced at the same culture conditions.

Celluloytic microorgnasims were isolated form the various sources and four of them were identified as Trichoderma roningi, Aspergillus niger, Penicillium chrysogenum, and Streptomyces sp. The induction of extracellular5 cellulase of these species in the liquid culture media containing carboxymethylcellulose (CMC) or Avicel as inducer showed that CMC was a better effective inducer for the production of CMCase(Cx cellulase component) as well as Avicelase(C cellulase component) than Avicel. It is believed that certain hydrolysis products of cellulose(CMC) could serve as an inducer for an enzyme synthesis. In T. roningi, Asp. niger, and Strptomyces sp., the optimum temperature of CNCase on CMC-culture medium was 50.deg. but temperature around 40.deg.C was found to be optimum for the activities of CMCase prepared from P.ehrysogenum. The optimum temperature for Avicelase activitiles on Avicel-culture media of T. roningi and P. chrysogenum was whereas temperature was found to be optimum for Avicelase from A.niger and Streptomyces sp. The optimal activities of these CNCase and Avicelase prepared from. T. ronigi, Pen.chrysogenum and Streptomyces sp. were found similarly to be at pH's around 5.4 and 6.0 while pH 4.8 was optimum for the activities of CMCase and Avicelase from A.niger, indicating that A.niger in acidic media would yield an enzyme of high activity.

A taxonomical sutdy was made on the Streptomyces species isolated from soils in this country, most of which were collected during the period from April, 1974 to July 1975. J.S.P. Methods (1966), I.S.P. Descriptions (1968-1972) and Bergy's Manual of Determinative Bacteriology (1974) were used for most of the experimental methods and identifications. As a result, 24 species were identified as follows ; S. albolongus, S galticri.S. Nashvillensis. S. showdoensis. S.norbonensis. S. flacocirens. S. resistomycificus. S> reshiriensis. S. chromofuscus. S. parvullus. S, chibaensis. S.canus. S. albulus. S. amlachiticus. S.griseoflavus. S. griscoincarnotus. .S. rubiginosus. S. bacillaris. S. setonill. S.intermedius. S. griseinus. S.subrutilus. S.reseosporus.

The insecticides, Dithane-M45 and copper sulfate, were introduced in this experiments to elucidate their effects on the nodulation of soybean plant(Glycine max Meer) by Rhizobium japonicum. The nodulation activity was inhibited in accordance with increase of their concentration.

This experiment was designed to study the effects of temperature on the peroxidase isoenzymes of a mesophilic microorganism, Escherichia coli (grown within biokinetic zone). Optimum temperature for the growth of E. coli was Three different temperatures, 20, 30 and were selected. And the isoenzyme patterns of peroxidase of E. coli, growth respectively at each temperature, were analysed by disc electrophoresis. The sample of 20.deg.C showed 4 bands, that of 30.deg.C, 5 bands and that of 40.deg.C, 6 bands. Two dark bands (higher molecular weight, 56,000 and 54,000) and two light bands (lower molecular weight, 11,500 and 10,000) were constant at all samples. But two intermediate bands (M.W.44,000 and 34,000) were variable ; at no banding pattern, one band 9M.W. 34,000) only at and at two bands were appeared. And the shifts of growth temperatures between 30.deg.C and 40.deg.C showed the alteration of the isoenzyme patterns ; the isoenzyme patterns of the smaple of tmeperature shift from to were same as that of 40.deg.C and vice versa.