More Details about

Target Name

Target Species

Application Notes

It is recommended to reconstitute the lyophilized Exendin Recombinant in sterile 18M?-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Specific Activity

The biological activity is determind by using the Van Hell Bioasssay described in the British Pharmacopeia.

Recommended Applications

Exenatide.

Reconstitution

P26349

Storage/Stability

Lyophilized Exendin Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Exendin-4 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Format

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The Exendin-4 peptide was lyophilized after extensive dialyses against 20mM PBS and contains 4% mannitol.

Preparation

Exendin-4 Recombinant is a novel 39-amino acids peptide having a molecular mass of 4186.7 Dalton, which shares 53%, sequence homology with GLP-17-36 amide and interacts with the same membrane receptor.
The Exendin-4 is purified by proprietary chromatographic techniques.

Expression System

Escherichia Coli.

Amino Acid Sequence

The sequence determined and was found to be His-Gly-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Leu-Ser-Lys-Gln-Met-Glu-Glu-Glu-Ala-Val-Arg-Leu-Phe-Ile-Glu-Trp-Leu-Lys-Asn-Gly-Gly-Pro-Ser-Ser-Gly-Ala-Pro-Pro-Pro-Ser.

Purification

Purity:

Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

More Information

Target Synonyms

Exenatide.

Uniprot Accession Number

P26349

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.