The 5X whole-genome shotgun sequences of strain FB1 were generated at Exelixis and assembled at the Center for Genome Research.

Two of the contigs in the previous release (contigs 1.246 & 1.251) have been identified as mitochondrial contigs. The contig names were changed as follows:

New name

Old name

Ustilago maydis mitochondria contig 1.1

Ustilago maydis contig 1.246

Ustilago maydis mitochondria contig 1.2

Ustilago maydis contig 1.251

June 20, 2003

The Ustilago maydis genome project is part of the Fungal Genome Initiative and represents a collaboration between Bayer CropScience, Exelixis Inc. and the Broad Institute of MIT and Harvard. Broad generated a 10X assembly using whole-genome shotgun sequences. Bayer CropScience contributed their genome sequence of U. maydis strain 521 generated from a minimal tiling path of overlapping BAC clones to be used as physical map to validate the 10X assembly generated at Broad and to anchor the scaffolds to the chromosomes. Exelixis provided their 5X whole-genome shotgun sequences of U. maydis strain FB1 to be aligned against our assembly to identify polymorphism between these two strains.

The initial release of our 10X sequence assembly of the Ustilago maydis assembly. The sequence traces from the Broad Institute sequencing can be downloaded from the NCBI trace repository. This Whole Genome Shotgun project has been deposited at DDBJ/EMBL/GenBank under the project accession AACP00000000. The version described in this release is the first version, AACP01000000.