pol beta and BRCA1 appeared to have a functional interaction, observed in both DT40 and human cell lines, in protecting cells against alkylating MMS-induced cytotoxicity; but pol beta had no protective effect in the absence of BRCA1. In vivo and in vitro assays for a base excision repair role of BRCA1 were negative.

pol beta contains a specific NLS (显示 ALDH1A2 抗体) sequence in the N-terminal lyase domain that promotes transport of the protein independent of its interaction partners. Active nuclear uptake allows development of a nuclear/cytosolic concentration gradient against a background of passive diffusion.

Data suggest that POLB activity is not essential for HIV-1 integration into human macrophages and other cells; limited cellular dNTP pools, but not POLB expression, are primary factor for HIV-1 DNA gap repair, particularly in non-dividing cells.

Polbeta was shown to inhibit the 3'->5' exonuclease (显示 EXOSC10 抗体) activity of APE1 (显示 APEX1 抗体) when both enzymes were added simultaneously and to insert the correct nucleotide into the gap arising after hydrolysis of AP site clustered with the benzo[a]pyrene adducts.

pol beta contains a specific NLS (显示 ALDH1A2 抗体) sequence in the N-terminal lyase domain that promotes transport of the protein independent of its interaction partners. Active nuclear uptake allows development of a nuclear/cytosolic concentration gradient against a background of passive diffusion.

pol beta plays an important role in bypassing a 5',8-cyclo-dA during DNA replication and repair.

we show here that heterozygosity for a Y265C mutation in Polbeta, a key polymerase in the BER pathway, is enough to significantly reduce both the number of expansions seen in paternal gametes and the extent of somatic expansion in some tissues

Results show that deficiency of either DNA polymerases beta or lambda or both results in a modest but significant decrease in V region somatic hypermutation (SHM (显示 CNTNAP1 抗体)) with no effect on mutation specificity, suggesting no direct role in SHM (显示 CNTNAP1 抗体).

Data indicate an association of ovarian stimulation with a downregulation of mRNAs encoding the base excision repair proteins APEX1 (显示 APEX1 抗体) and POLB as well as the 5-methyl-CpG-binding domain protein MBD3 (显示 MBD3 抗体) in individual morula embryos.

POLB 抗原简介

Antigen Summary

The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date.