Figure 4: Immunostaining of hEnSCs differential markers. Expression of neuron markers including Nestin, MAP-2 and β-tubulin was analyzed in FLX induced differentiation after 10 d. The expression of CD11b as a glial marker was not obvious. The size bar is 10 μm (a). Immunostaining of LY294002 treated hEnSCs after culture in FLX supplemented medium. There is not an expression of neuron markers in FLX induced differentiation after 10 d (b).