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Characterization and purification of alkaline phosphatase from Elephas trogontherii (Steppe elephant) bonehttp://nopr.niscair.res.in/handle/123456789/3522
Title: Characterization and purification of alkaline phosphatase from Elephas trogontherii (Steppe elephant) bone
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<br/>Authors: Demir, Yaşar; Yildirim, Safinur; Demir, Nazan
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<br/>Abstract: Four isozymes of alkaline phosphatase (AP) were purified from Elephas trogontherii (Steppe elephant) from different locations in the bone (outer and inner peripheral, cytosolic, and integral) using Sephadex G-200 gel filtration and TEAE-cellulose anion-exchange chromatography. The specimen was obtained from Erzurum Museum and its age was approx. 0.3-0.5 million years old. No fungi or bacteria were present in the bone sample. The enzyme activity was determined by using p-nitrophenylphosphate as a substrate. SDS-PAGE of all the isozymes gave a single band at the same location. The molecular mass of the four isozymes as determined by using gel filtration was about 60 kDa. Optimum pHs for the four isozymes were between 8-8.5. The optimum temperatures of the isozymes were: outer peripheral, 37.5ºC, cytosolic, 37.5ºC, inner peripheral, 35ºC and integral, 40ºC. The values of Vmax and Km, as well different optimum temperatures indicated that isozymes were structurally different.
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<br/>Page(s): 182-185Phytomitogen induced changes in levels of inositol phosphates in the bovine lymphocyteshttp://nopr.niscair.res.in/handle/123456789/3521
Title: Phytomitogen induced changes in levels of inositol phosphates in the bovine lymphocytes
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<br/>Authors: Dhingra, Jyotsna Behl nee; Verma, N K; Behl, Rahul; Prasad, S K; Ahlawat, S P S
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<br/>Abstract: The changes in levels of inositol phosphates and phosphoinositides were studied in the bovine lymphocytes, in response to phytomitogens (lectins)-concanavalinA (conA) and phytohaemagglutinin (PHA). Addition of conA and PHA resulted in a rapid increase in the cpm of total inositol phosphates (from 8599 ± 100 cpm/2  10⁶ cells to 11228 ± 126 cpm/2 10⁶ and 9758 ± 100 cpm/2 10⁶ cells, respectively) at 1 min after mitogen stimulation. There was a concomitant decrease in the phosphatidylinositol levels at 1 min, which continued up to 5 min. At 1 min of stimulation, inositol diphosphate fraction exhibited maximum increase, as compared to inositol mono- and triphosphates, suggesting that it contributed the most towards the overall increase in the total inositol phosphates levels. Results suggest that bovine lymphocytes respond to phytomitogens with a rapid turnover of phosphoinositides.
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<br/>Page(s): 173-177Determination of serum lactate with alkylamine glass bound lactate oxidasehttp://nopr.niscair.res.in/handle/123456789/3520
Title: Determination of serum lactate with alkylamine glass bound lactate oxidase
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<br/>Authors: Suman; Pundir, C S
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<br/>Abstract: Commercial lactate oxidase (Lactate:O₂; oxidoreductase EC 1.1.3.2) from Pediococcus species was immobilized on to al-kylamine glass beads (pore diameter 55 nm) through glutaralde-hyde coupling with a conjugation yield of 3.2 mg/g support and 105% retention of initial activity. Immobilized enzyme showed maximum activity at pH 6.5, when incubated at 40ºC for 12 min and was used for determination of lactic acid in serum. The H₂O₂ generated from serum lactate by immobilized enzyme was meas-ured colorimetrically at 565 nm by its oxidative coupling with
4-aminoantipyrine and N,N'-dimethyaniline catalyzed by horse-radish peroxidase. A linear relationship was observed between A₅₆₅ and lactic acid concentration ranging from 0.075 mM to
10 mM. The minimum detection limit of the method was
0.075 mM, which was better than that of enzymic colorimetric method employing free enzyme (0.2 mM). Within day and
between day coefficient of variations were <8.0% and <19%,
respec-tively. Serum lactic acid values determined by the present method were in good correlation (r = 0.99) with the currently used enzymic colorimetric method. The cost of lactate determination for 100 serum samples was less, as compared with Sigma kit method.
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<br/>Page(s): 186-189Oxidative stress in relation to lipid profiles in different stages of breast cancerhttp://nopr.niscair.res.in/handle/123456789/3519
Title: Oxidative stress in relation to lipid profiles in different stages of breast cancer
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<br/>Authors: Gönenç, Aymelek; Tokgöz, Devrim; Aslan, Sabahattin; Torun, Meral
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<br/>Abstract: The changes in the levels of MDA, nitrite, vit. E, lipids (total cholesterol and triglycerides) and lipoproteins (HDL and LDL cholesterol) were estimated among breast cancer patients, in relation to different clinical stages (stage I to IV). MDA and nitrite levels were increased in breast cancer patients, irrespective of clinical stage, as compared to controls (p<0.01). Their levels were also significantly elevated from stage III to stage IV (p<0.05). In contrast, vit. E levels were decreased in all stages, as compared to control group (p<0.05), the decrease was more pronounced in stage II and IV. Compared to controls, serum triglycerides were elevated in all patient groups (p<0.05); the maximum increase was in stage IV. HDL-cholesterol decreased in all stages, when compared with control group (p<0.05). These findings support the hypothesis that reactive oxygen and nitrogen species are increased in breast cancer, especially metastases and may cause consumption of vit. E.
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<br/>Page(s): 190-194