RNA Sequencing Service

RNA Sequencing Service

RNA-Seq for mRNA at the sequencing depths detects and quantifies both known and novel transcripts, isoforms, variants and other features without the limitation of established transcriptome for the species. Using Illumina HiSeq platform, Arraystar offers integrated RNA Sequencing service of mRNAs, from sequencing library preparation to comprehensive data analysis.

Description

Benefits

Data Analysis

Publications

Service Name

Data Qty

Read Length

Seq Depth

Application

Price

RNA Sequencing Service

6G

150bp Paired -end

40M (20M fragments)

Excellent coverage for protein coding mRNAs

RNA Sequencing Service

12G

150bp Paired -end

80M (40M fragments)

Best detection of RNA types or transcript isoforms

Arraystar RNA Sequencing profiles mRNAs with excellent coverage. The sequencing library is constructed using dUTP strategy for strand-specific sequencing and unbiased even read distribution along the transcript from 5’ to 3’ end. The paired-end chemistry and long read length allow effective analysis of alternatively spliced transcript isoforms. A comprehensive and easy-to-read mRNA-Seq report is provided along with the complete raw data files.

Degraded RNA sequencing by alternative procedure

mRNA-Seq normally uses oligo(dT) for mRNA enrichment. However, RNAs in many precious sample sources such as FFPE clinical samples may have been partially degraded. The fragmented mRNAs will be lost during standard oligo(dT) enrichment We provide alternative rRNA depletion procedure to remove abundant rRNAs while preserve the mRNA fragments, producing good results even for degraded samples.

The alternative rRNA deletion sample prep should also be used if ncRNA analysis is part of your research interest, as ncRNAs often lack a poly(A) tail.

Please inform us at the initial project consultation if your RNA samples may be partially degraded or ncRNA is to be included in the analysis.

Arraystar has perfected the RNA-Seq technologies, from sample prep, library construction, sequencing and data analysis to obtain upmost sensitivity and accuracy for precise, reliable quantification of differential expression as well as detection of novel transcripts and isoforms.

• Strand-specific and even read distribution
dUTP incorporation in sequencing library construction unambiguously determines sense or antisense RNA transcripts in strand-specific sequencing. The sequencing reads are uniformly and evenly distributed along the entire lengths of the transcripts (Fig. 1)