A flight number, when combined with the name of the airline and the date, identifies a particular flight. This callsign should not be confused with the tail number of the aircraft, although both can be used as a call-sign as used in general aviation. A particular aircraft may fly several different flights in one day, and different aircraft may be used for the same flight number on successive days.

A number of conventions have been developed for defining flight numbers, although these vary widely from airline to airline. Eastbound and northbound flights are traditionally assigned even numbers, while westbound and southbound flights have odd numbers. Other airlines will use an odd number for an outbound flight and use the next even number for the reverse inbound flight. For destinations served by multiple flights per day, numbers tend to increase during the day. Hence, a flight from point A to point B might be flight 101 and the return flight from B to A would be 102, while the next pair of flights on the same route would usually be assigned codes 103 and 104.

Likewise, flight numbers larger than 9000 are usually referred to as ferry flights, that carry no passengers and are only to move an aircraft from point A to point B, where it is supposed to start a new commercial flight. Flight numbers starting with 8 are often used for charter flights, but it always depends on the commercial carrier's choice.

Note that, although 'flight number' is the term used colloquially, the official term as defined in the Standard Schedules Information Manual (SSIM) published annually by the International Air Transport Association (IATA) Schedules Information Standards Committee (SISC), is flight code. Officially the term 'flight number' refers to the numeric part (up to four digits) of a flight code. For example, in the flight codes BA2490 and BA2491A, "2490" and "2491" are flight numbers. Even within the airline and airport industry, it is common to use the colloquial term rather than the official term.

Flight numbers are also sometimes used for spacecraft, though a flight number for an expendable rocket (say, Ariane 5 Flight 501) might more reasonably be called the serial number of the vehicle used, since an expendable rocket can only be launched once. Space Shuttle missions used numbers with the STS prefix, for example, STS-93.