GPRPQGATVSLSETVQKWREYRHQCQRFLTEAPLLATGLFCNRTFDDYACWPDGPPGSFVNVSCPWYLPWASSVLQGHVYRFCTAEGIWLHKDNSSLPWRDLSECEESKQGERNNote: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

17.1kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Study found GLP-1/GLP-1R signaling in the brainstem to be required for the central mediation of cancer anorexia-cachexia syndrome in hepatoma tumor-bearing rats. A blockade of brainstem GLP-1 not only attenuated anorexia, but also body weight loss and muscle wasting. PMID: 29247677

These findings indicate that sitagliptin treatment regulates GLP-1R and CB-1R gene expressions, which are associated with appetite regulation in diabetic rat, and may decrease oxidative stress and liver tissue damage. PMID: 28599244

Nucleus tractus solitarius GLP-1R signaling is required for the control of food intake and motivation to feed. PMID: 27782127

The findings of this study indicated that increased activation of NTS GLP-1-expressing neurons by corticosterone may represent a homeostatic response to cocaine taking, thereby reducing the reinforcing efficacy of cocaine. PMID: 26675243

Results strongly implicate the GLP-1R in mediating the unconditioned suppression of dopamine signaling by the emetic agent LiCl and support the GLP-1R as a target in the treatment of maladaptive aversive associations PMID: 26211731

hindbrain GLP1 neurons in rats are equipped to store glutamate in synaptic vesicles, and likely co-release both glutamate and GLP1 from axon varicosities and terminals in the hypothalamus and other brain regions PMID: 25012114

Glucagon-like peptide-1 receptor (GLP-1R) agonists reduce food intake and are approved by the Food and Drug Administration for the treatment of obesity, but the cellular mechanisms underlying the anorectic effects of GLP-1 require further investigation. PMID: 27013681

GLP-1 receptors are located in the renal vasculature, including afferent arterioles. Activation of these receptors reduces the autoregulatory response of afferent arterioles to acute pressure increases and increases RBF in normotensive rats. PMID: 25656368

Blockade of endogenous GLP-1R signaling in the VTA and NAc core resulted in a significant increase in food intake, establishing a physiological relevance for GLP-1 signaling in the mesolimbic reward system. PMID: 22128031

Endogenous GLP-1 receptor signaling is necessary for the reduction in food intake produced by jejunal linoleic acid infusions. PMID: 21917638

these findings indicated that glucose fluctuation reduces GLP-1R expression through ER stress more profoundly than sustained hyperglycemia, which may contribute to the diminished response of GLP-1. PMID: 21945929

GLP-1r are present on nerve terminals in hepatic portal bed, and GLP-1 antagonism localized to this region impairs glucose tolerance. These data are consistent with an important component of neural mediation of GLP-1 action. (GLP-1) PMID: 17584962

The major determinant of exendin-4/glucagon-like peptide 1 differential affinity at the rat glucagon-like peptide 1 receptor N-terminal domain is a hydrogen bond from SER-32 of exendin-4. PMID: 20649595

Over-expression of beta-cell Glp1r increases the potency and efficacy of D-glucose as a stimulator of RIP 1-Luc. It may be a possible strategy by which beta-cell lines may be engineered for efficient glucose-dependent insulin biosynthesis and secretion. PMID: 11845326

rats overexpressing GLP-1R in the hippocampus show improved learning and memory PMID: 12925848

Gene expression of the GLP-1 receptor was clearly detected by both RT-PCR and Northern blot analysis. In situ hybridization study confirmed that the expression occurs in neuronal cells of the nodose ganglion. PMID: 14766323

structure-activity of binding with agonists GLP-1 and exendin-4 PMID: 14965273

GLP-1r are present on nerve terminals in hepatic portal bed, and GLP-1 antagonism localized to this region impairs glucose tolerance. These data are consistent with an important component of neural mediation of GLP-1 action. PMID: 17584962

Geniposide, an agonist for GLP-1 receptor, regulates expression of anti-oxidative proteins including HO-1 and Bcl-2 by activating the transcriptor of p90RSK via MAPK signaling pathway in PC12 cells. PMID: 17629357

Cholangiocytes are susceptible to the activation of GLP-1R and respond with increased proliferation and functional activity. PMID: 17631146

The remarkable levels of GLP-1 in intestinal lymph demonstrate the potential for lymphatic sampling as a more sensitive means of studying the secretory physiology of this hormone in vivo. PMID: 17898126

Stimulating effect of leptin on GLP-1R expression, with no changes in neuropeptide y-induced activity, could enhance the anorexic actions generated through this receptor. PMID: 18078857

caudal brainstem processing is sufficient for mediating the suppression of intake, core temperature, and gastric emptying rates as well as tachycardia triggered by peripheral GLP-1R activation. PMID: 18420740

Our data suggest that the GLP-1 receptor is restricted to the pancreatic beta cells. PMID: 18541709