Restriction of PCR product

If you had a bright DNA band after PCR, use 20μL of the product. If you had a faint band, lyophilize the 50μL from the previous step and add 20μL water. The restriction enzymes that I need for pQE-80 are: BamHI and HindIII

Start protocol first thing in the morning

Do the restriction in a total 50μL mix

normally choose the buffer that is good for both enzymes, but NEB says to do them sequentially