With the advent of functional genomics, a major chal-lenge is the analysis and interpretation of the ever-increasing se-quencing data. deFUME is an easy-to-use web-based interface for processing and annotation of functional metagenomics sequencing data, tailored to meet the requirements of non-bioinformaticians. The web-server integrates multiple analysis steps into one single work-flow: read assembly, open reading frame prediction and finally anno-tation with BLAST, InterPro and GO classifiers. This provides a fast track from raw sequence to a comprehensive visual data overview that facilitates effortless inspection of gene function, clustering and distribution.