Real Experiments • 3D Astigmatism

3D astigmatic image of microtubules imaged using dSTORM with a cylindrical lens (112'683 frames).
Microtubules in U-2 OS cells, labeled with anti-alpha tubulin primary and Alexa Fluor 647-coupled secondary antibodies.
Calibration data for the tubulin dataset: fluorescent beads adsorbed to a glass coverslide in water are stepped in Z at high SNR.

3D astigmatic dSTORM image of nuclear pore complex.
Nup107-SNAP-BG-AF647 in U-2 OS cells, imaged using dSTORM with a cylindrical lens (150'000 frames).
Calibration data for the dataset: fluorescent beads adsorbed to a glass coverslide in water are stepped in Z at high SNR.

Realistic Simulations • 3D • Experimental PSF

The experimental datasets consist of sequences of frames from real stained biologicial samples.
A Z-stack of beads is provided for the axial calibration. The same 3D simulated structure is imaged in different experimental conditions
and for different modality: 2D, 3D astigmatism (AS), 3D biplane (BP), and 3D double-helix (DH).
As a testing phase, we have also the quad-plane (QP) datasets for high-density conditions. The training datasets are provided with ground-truth localizations.

Microtubules • Contest datasets

Published June 7, 2016 as training dataset for the SMLM challenge 2016