Available images

A cell cytospin of human lung cancer cell line A549 was fixed with cold acetone for 90 seconds and air-dried. Cells were incubated with the blocking buffer (PBS containing 5% FCS) for 30 minutes at room temperature. Cells were then washed once in PBS and incubated with primary antibody, diluted 1:100 in the blocking buffer, for 30 minutes. Slides were washed 3X in PBS and incubated with Goat anti-sheep conjugated to Alexa-488, diluted 1:200 in blocking buffer, for 30 minutes at room temperature in the dark. Slides were washed as above and mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 µg/ml was used for nuclear counterstaining. Fluorescent cell staining were analysed using a Olympus microscope and the analysis LS Research software.

A cell cytospin of human lung cancer cell line A549 was fixed with cold acetone for 90 seconds and air-dried. Cells were incubated with the blocking buffer (PBS containing 5% FCS) for 30 minutes at room temperature. Cells were then washed once in PBS and incubated with primary antibody, diluted 1:100 in the blocking buffer, for 30 minutes. Slides were washed 3X in PBS and incubated with Goat anti-sheep conjugated to Alexa-488, diluted 1:200 in blocking buffer, for 30 minutes at room temperature in the dark. Slides were washed as above and mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 µg/ml was used for nuclear counterstaining. Fluorescent cell staining were analysed using a Olympus microscope and the analysis LS Research software.

Handling

Handling

Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice

Avoid freeze and thaw cycles.

Storage

4 °C/-20 °C

Storage Comment

Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date

12 months

Images

Images

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A cell cytospin of human lung cancer cell line A549 was fixed with cold acetone for 9...

A cell cytospin of human lung cancer cell line A549 was fixed with cold acetone for 90 seconds and air-dried. Cells were incubated with the blocking buffer (PBS containing 5% FCS) for 30 minutes at room temperature. Cells were then washed once in PBS and incubated with primary antibody, diluted 1:100 in the blocking buffer, for 30 minutes. Slides were washed 3X in PBS and incubated with Goat anti-sheep conjugated to Alexa-488, diluted 1:200 in blocking buffer, for 30 minutes at room temperature in the dark. Slides were washed as above and mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 µg/ml was used for nuclear counterstaining. Fluorescent cell staining were analysed using a Olympus microscope and the analysis LS Research software.

A cell cytospin of human lung cancer cell line A549 was fixed with cold acetone for 90 seconds and air-dried. Cells were incubated with the blocking buffer (PBS containing 5% FCS) for 30 minutes at room temperature. Cells were then washed once in PBS and incubated with primary antibody, diluted 1:100 in the blocking buffer, for 30 minutes. Slides were washed 3X in PBS and incubated with Goat anti-sheep conjugated to Alexa-488, diluted 1:200 in blocking buffer, for 30 minutes at room temperature in the dark. Slides were washed as above and mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 µg/ml was used for nuclear counterstaining. Fluorescent cell staining were analysed using a Olympus microscope and the analysis LS Research software.

Xu, Moebius, Gill, Montell: "Regulation of melastatin, a TRP-related protein, through interaction with a cytoplasmic isoform." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 19, pp. 10692-7, 2001 (PubMed).