Application Information: NALP1 antibody

Immunoprecipitation: use at a concentration of 3.0 - 5.0 μg / extract from 10^7 cells. Western Blotting: use at a concentration of 0.5 - 2.0 μg/ml. ELISA: use at a concentration of 0.1 - 1.0 μg/ml. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of approximately 165 kDa in rat brain tissue lysate, which corresponds to the predicted molecular weight of DEFCAP. Sample wasboiled prior to subjecting on 7.5% SDS-PAGE. An ~80 kDa band was also observed in this blot.

Form Supplied

Liquid

Concentration

2
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Immunogen affinity purified

Specifications: NALP1 antibody

Full Name

NLR family, pyrin domain containing 1

Product Description

Rabbit polyclonal to NALP1

Background

Death Effector Filament-forming Ced 4 like Apoptosis Protein (DEFCAP) is a novel apoptotic protein of the mammalian Ced 4 family. It has two isoforms: DEFCAP-Land DEFCAP-S. The deduced amino acid sequences of these two alternately spliced isoforms only differ by 44amino acids at the C-terminus. DEFCAP contains a caspase recruitment domain (CARD), a domain that with the proper stimulation can recruit caspases to form cytoplasmic structures called death effector filaments, which in turn initiate the apoptotic signaling pathway.