Materials

Basic Protocol 1: Large‐Scale Generation of ND VLPs

Tissue culture cells (ELL‐0 are preferable but COS7, CHO, or 293T cell lines are acceptable; all are available at http://www.atcc.org)

Cell culture medium such as DMEM with appropriate supplementation (see recipe) for the cells to be used (see instructions from ATCC)

Plasmids containing cDNAs encoding NDV NP, M, F, and HN proteins (prepared using Qiagen Endo‐free kit or the equivalent; use
of the pCAGGS vector as the expression vector is highly recommended—this plasmid may be obtained from the Belgian Coordinated
Collection of Microorganisms, BCCM/LMBP; bccm.lmbp@dmbr.UGent.be)