You have truncated protein domain that won't express? - If you wanted to express a protein but failed because domain not intac (May/01/2011 )

I am looking for proteins that you wanted to express but it failed because the protein contained a (globular) domain and the protein aggregated. Usually such proteins/experiments won't be published by their very nature. However, we are collecting such proteins/domains to test our server that would predict exactly such events. Could you please, send me your misbehaving protein or if you know about such a failed experiment, that would be great, too. Please, let me know! Here is our paper about the background: http://www.ncbi.nlm.nih.gov/pubmed/20972208 We are developing now a server and if you could send a valid example (either positive or negative), we'd be happy to acknowledge it in our upcoming paper about the server. Thanks, H.Hegyi

-drckitty-

In case I was ambiguous in my previous post: we are looking for proteins with TRUNCATED globular domain(s) where the protein aggregation was probably caused by the domain truncation.

Your failed protein expression would be greatly appreciated (and probably I am the only one who'd be interested in such a failed experiment).

Thanks,
H.Hegyi

drckitty on Sun May 1 21:49:35 2011 said:

I am looking for proteins that you wanted to express but it failed because the protein contained a (globular) domain and the protein aggregated. Usually such proteins/experiments won't be published by their very nature. However, we are collecting such proteins/domains to test our server that would predict exactly such events. Could you please, send me your misbehaving protein or if you know about such a failed experiment, that would be great, too. Please, let me know! Here is our paper about the background: http://www.ncbi.nlm.nih.gov/pubmed/20972208 We are developing now a server and if you could send a valid example (either positive or negative), we'd be happy to acknowledge it in our upcoming paper about the server. Thanks, H.Hegyi