Efficient. Flexible. Transformative.

The 2018 Attune NxT Flow Cytometer system makes multiparametric flow cytometry available to both new and experienced researchers. Experience the difference acoustic assisted hydrodynamic focusing technology brings to your workflow. Designed for faster experimental run times, this compact flow cytometer offers proven, rapid and accurate analysis for a broad range of sample types.

The 40-page Flow Cytometer Evaluation Guide will enable a better understanding of the components and capabilities of various flow cytometers for objective comparison of instruments from several manufacturers.

Automation for any lab

Improve your workflow for fully automated sample acquisition from 96-well and 384-well microtiter plates. There are no physical changes to the system needed in order to run plate based experiments—everything is controlled in the software.

2018 Attune NxT instrument options

This grow-with-you system can be customized with up to 4 lasers and 14 colors and now up to 6 channels off of the violet laser. The optical system was designed to provide optimal signal and has the range to detect very bright as well as dim signals. The Attune NxT Flow Cytometer allows researchers the freedom to build multicolor panels, accommodating common fluorophores and dyes. Build your multicolor panel for the Attune NxT flow cytometer with our superior reagents and antibodies.

* Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel. † Attune NxT Flow Cytometer owners wishing to purchase a laser upgrade should request a quote here: Yellow Laser Upgrade, Violet Laser Upgrade, Red Laser Upgrade, or Green Laser Upgrade. After we receive your quote request, a technical sales specialist will contact you about upgrade options.

Are you comparing the specifications of more than one flow cytometer?

A specification is derived from a specific test or calculation, but these tests aren’t standardized across instrument developers and may be misleading in a side-by-side comparison because their values may have been calculated in different ways, despite sharing the same terminology. This 8-page Comparing Technical Specification Documents resource offers helpful tips on deciphering variations.

The Invitrogen Attune NxT Flow Cytometer was developed with acoustic-assisted hydrodynamic focusing technology, and advanced fluidics designed to minimize clogging and effectively handle a broad range of cell types. This allows for a higher degree of data, detail, and throughput that enables processing of a large range of sample types, including large clumpy cells, samples with a low concentration of cells, and precious samples, more quickly and accurately than ever before with no loss in data quality.

The following applications were performed using the Attune NxT Flow Cytometer.

Updates relating to all service bulletins relevant to instrument configuration

Checks completed during planned maintenance visit:

System maintenance

visually inspect all cables

clean the interior and the exterior of unit

clean the cove tray and visor

clean the tube lifter

check the tube lifter for proper operation

Computer maintenance

run the disk cleanup

defragment the disk, only if the operating system indicates that it is recommended

Fluidics system maintenance

replace the SIP tube

inspect the instrument internally for any traces of salt residue

check for internal leaks and damage at all fittings, valves, pumps and syringe pump

replace the 1 mL syringe

run the system decontamination script, if not previously done by the customer

replace both 0.2 micron filters, if not previously done by the customer

Optics maintenance

inspect all optical filters. Clean only if necessary

verify the laser alignment, including the pinholes and blocker bar

System diagnostics

run the System Test and ensure that everything passes

ensure that the latest Attune NxT Software version is installed

check leak detector functionality

record laser hours

Attune NxT Autosampler maintenance (if applicable to unit)

run the Attune NxT Autosampler calibration script

replace syringe

Instrument performance

ensure that the baseline and performance tests pass the specifications

Compliance services

Get timely, cost-effective, and audit-ready documentation managed by a compliance specialist to help ensure your Attune NxT Flow Cytometer system is installed, operating, and performing to the manufacturer’s specifications.

Education services

Application and instrument training programs are available at our training centers located throughout the world, within your lab, or through web-based instruction. We offer a wide range of courses to build the skills of cellular analysis professionals and users of the Attune NxT Flow Cytometer:

Training Center Courses—get away from the day-to-day responsibilities of the lab and dedicate time to focus on your education needs. Courses are hosted at Thermo Fisher Scientific Training Center sites to provide professional, interactive education that includes classroom instruction and hands-on training in the laboratory.

Customer Site/content—courses are offered at your location by a Field Application Scientist (FAS) to provide professional, interactive education that includes hands-on instruction in your laboratory.

LabCoat Live—learn a specific application in as little as 1 week. Courses provide interactive education via online lectures and a self-paced hands-on experiment using provided protocols and reagents that we ship to your lab.

eLearning—discover new areas of interest and learn about life science topics at your own pace; courses provide education via on-demand, pre-recorded webinars.

Instrument management

Manage your instrument use and care online with Instrument Management. Whether you are responsible for one instrument or hundreds of instruments, Instrument Management enables you to easily manage your instrument use and care online.

24/7 access to complete instrument service history and field service reports

The 40-page Flow Cytometer Evaluation Guide will enable a better understanding of the components and capabilities of various flow cytometers for objective comparison of instruments from several manufacturers.

Automation for any lab

Improve your workflow for fully automated sample acquisition from 96-well and 384-well microtiter plates. There are no physical changes to the system needed in order to run plate based experiments—everything is controlled in the software.

2018 Attune NxT instrument options

This grow-with-you system can be customized with up to 4 lasers and 14 colors and now up to 6 channels off of the violet laser. The optical system was designed to provide optimal signal and has the range to detect very bright as well as dim signals. The Attune NxT Flow Cytometer allows researchers the freedom to build multicolor panels, accommodating common fluorophores and dyes. Build your multicolor panel for the Attune NxT flow cytometer with our superior reagents and antibodies.

* Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel. † Attune NxT Flow Cytometer owners wishing to purchase a laser upgrade should request a quote here: Yellow Laser Upgrade, Violet Laser Upgrade, Red Laser Upgrade, or Green Laser Upgrade. After we receive your quote request, a technical sales specialist will contact you about upgrade options.

Are you comparing the specifications of more than one flow cytometer?

A specification is derived from a specific test or calculation, but these tests aren’t standardized across instrument developers and may be misleading in a side-by-side comparison because their values may have been calculated in different ways, despite sharing the same terminology. This 8-page Comparing Technical Specification Documents resource offers helpful tips on deciphering variations.

The Invitrogen Attune NxT Flow Cytometer was developed with acoustic-assisted hydrodynamic focusing technology, and advanced fluidics designed to minimize clogging and effectively handle a broad range of cell types. This allows for a higher degree of data, detail, and throughput that enables processing of a large range of sample types, including large clumpy cells, samples with a low concentration of cells, and precious samples, more quickly and accurately than ever before with no loss in data quality.

The following applications were performed using the Attune NxT Flow Cytometer.

Updates relating to all service bulletins relevant to instrument configuration

Checks completed during planned maintenance visit:

System maintenance

visually inspect all cables

clean the interior and the exterior of unit

clean the cove tray and visor

clean the tube lifter

check the tube lifter for proper operation

Computer maintenance

run the disk cleanup

defragment the disk, only if the operating system indicates that it is recommended

Fluidics system maintenance

replace the SIP tube

inspect the instrument internally for any traces of salt residue

check for internal leaks and damage at all fittings, valves, pumps and syringe pump

replace the 1 mL syringe

run the system decontamination script, if not previously done by the customer

replace both 0.2 micron filters, if not previously done by the customer

Optics maintenance

inspect all optical filters. Clean only if necessary

verify the laser alignment, including the pinholes and blocker bar

System diagnostics

run the System Test and ensure that everything passes

ensure that the latest Attune NxT Software version is installed

check leak detector functionality

record laser hours

Attune NxT Autosampler maintenance (if applicable to unit)

run the Attune NxT Autosampler calibration script

replace syringe

Instrument performance

ensure that the baseline and performance tests pass the specifications

Compliance services

Get timely, cost-effective, and audit-ready documentation managed by a compliance specialist to help ensure your Attune NxT Flow Cytometer system is installed, operating, and performing to the manufacturer’s specifications.

Education services

Application and instrument training programs are available at our training centers located throughout the world, within your lab, or through web-based instruction. We offer a wide range of courses to build the skills of cellular analysis professionals and users of the Attune NxT Flow Cytometer:

Training Center Courses—get away from the day-to-day responsibilities of the lab and dedicate time to focus on your education needs. Courses are hosted at Thermo Fisher Scientific Training Center sites to provide professional, interactive education that includes classroom instruction and hands-on training in the laboratory.

Customer Site/content—courses are offered at your location by a Field Application Scientist (FAS) to provide professional, interactive education that includes hands-on instruction in your laboratory.

LabCoat Live—learn a specific application in as little as 1 week. Courses provide interactive education via online lectures and a self-paced hands-on experiment using provided protocols and reagents that we ship to your lab.

eLearning—discover new areas of interest and learn about life science topics at your own pace; courses provide education via on-demand, pre-recorded webinars.

Instrument management

Manage your instrument use and care online with Instrument Management. Whether you are responsible for one instrument or hundreds of instruments, Instrument Management enables you to easily manage your instrument use and care online.

24/7 access to complete instrument service history and field service reports