Application Information: NCF4 antibody, Internal

Approx. 38kDa band observed in lysates of cell line Daudi (calculated MW of 39.0kDa according to NP_000622.2 and 38.8kDa according to NP_038202.1). In transfected HEK293 transiently expressing P40PHOX a band of approx. 42kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1µg/ml. In paraffin embedded Human Tonsil shows staining of the cytoplasm in some cells. Recommended concentration, 2-4µg/ml. This antibody is expected to recognise both reported isoforms (NP_000622.2 and NP_038202.1).

Form Supplied

Liquid

Concentration

0.5
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Specifications: NCF4 antibody, Internal

Full Name

neutrophil cytosolic factor 4, 40kDa

Product Description

Goat polyclonal antibody to NCF4

Background

Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.