To estimate the number of carriers of the c. The assumed moments to the most recent ancestral gene copy had been drawn from the distribution acquired above, and simulations were performed with the condition that the present era includes at least four carriers. Microtomographic pictures of the skull of the index individual were obtained at the Natural History Museum in London with the use of the HMX ST 225 cone-beam system and were virtually dissected by using VGStudio MAX 2.0 . Results Two teeth were removed from the skull of the index individual. Following the extraction of DNA, polymerase-chain-reaction amplification and sequencing had been performed on both tooth samples for the spot flanking the R304 site in four different reactions.