Fig. 1

Distribution of polySia in HUVEC. a PolySia was visualized in unpermeabilized HUVEC using mAb 735 in combination with an Alexa Fluor labeled secondary antibody. b In addition, polySia staining was performed after fixation and permeabilization using ice cold ethanol. c To prove the specificity of the antibody recognition, in a parallel set HUVEC were treated with endoN to digest polySia chains. Scale bar 100 µm