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Miltenyi Biotec distribution:

As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is
committed to providing our customers around the world with the highest quality products.
In addition to direct selling in more than 20 countries in North America, Europe and
Asia/Pacific, Miltenyi Biotec also provides support for our customers through an
extensive distributor network covering dozens of additional countries.

As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is
committed to providing our customers around the world with the highest quality products.
In addition to direct selling in more than 20 countries in North America, Europe and
Asia/Pacific, Miltenyi Biotec also provides support for our customers through an
extensive distributor network covering dozens of additional countries.

Anti-IgD MicroBeads, human

Applications

Enrichment or depletion of naïve B cells that express surface IgD can be achieved by sequential cell sorting using Anti-IgD MicroBeads. After the enrichment of B cells with the B Cell Isolation Kit II, IgD-expressing naive B cells are specifically labeled and isolated with Anti-IgD MicroBeads.

Columns

Figure 1

IgD

+

cells were isolated from human PBMCs using Anti-IgD MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD23-PE (# 130-094-505) as well as with Anti-IgD-APC (# 130-094-553) and analyzed by flow cytometry using the MACSQuant

®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.