This course describes the Nextera Flex for Enrichment technology, identifies the items needed, defines the various steps in the library preparation and enrichment steps, and provides an overview for sequencing and analysis.

After completing this course, you will be able to create an Illumina Custom Enrichment panel in DesignStudio. This course provides an introduction to DesignStudio and Illumina Custom Enrichment panels, guides you through the steps of creating and ordering a custom enrichment panel, and provides additional resources to get you started.

By the end of this course, you will be able to: prepare your lab for the arrival and installation of the iSeq 100 system, unpack and install the instrument, identify the test components required to perform first time setup, and perform first time setup of your instrument.

This course describes the AmpliSeq for Illumina assay technology, lists the three types of panels, introduces the steps in the amplicon sequencing workflow, and lists the analysis options for amplicon sequencing data.

This course identifies the items needed for the AmpliSeq for Illumina library prep protocol, introduces pool planning resources for a multiplexed amplicon run, demonstrates the steps in the library prep protocol, and lists best practices for the protocol.

After completing this course, you will be able to create an AmpliSeq for Illumina custom DNA panel in DesignStudio. This course provides an introduction to DesignStudio, guides you through the steps of creating a custom DNA panel, and provides tips and additional resources to get you started.

By the end of this course, you will be able to identify the Nextera DNA Flex kit, describe how the protocol works, identify what is needed to complete the protocol, and define the various steps in the library preparation workflow.

By the end of this course, you wiill be able to: identify the items needed for the protocol, describe the steps in the library prep protocol, describe the steps in the enrichment protocol, and list best practices for performing the protocol.

This course introduces lab requirements, computing requirements, and delivery considerations for the NovaSeq 6000 System. It also discusses the consumables required for running and maintaining the instrument.

This course describes the Rapid Run Mode available for HiSeq 2500 or HiSeq 1500. It identifies what is needed to perform a Rapid Run, describes the options for clustering the 2-lane flow cell, and lists ways to reduce data storage requirements for your HiSeq runs.

This course introduces four options for monitoring your NextSeq run. It also describes key metrics in NextSeq Control Software (NCS), Local Run Manager, Sequencing Analysis Viewer (SAV), and BaseSpace Sequence Hub.

After completing this course you will be able to describe the lab environment required to use MiSeq, list key components of MiSeq, identify the network considerations for MiSeq, and list the user-supplied consumables required for running and maintaining MiSeq.

After completing this course you will be able to identify the MiSeq run setup options, describe how to prepare the MiSeq reagent cartridge and load libraries, and list the steps to start a sequencing run with MiSeq Control Software (MCS).

After completing this course you will have an understanding of the demultiplexing and alignment processes and program interfaces that are common to all workflows in MiSeq Reporter. You will also be able to briefly describe the utility of the supported MiSeq Reporter workflows.

After completing this course you will be able to list the MiSeq data analysis tools, briefly describe the function of each tool, and list some considerations when choosing your MiSeq data analysis tool.

After completing this course you will be able to list two options for monitoring your MiSeq run, describe key metrics in MiSeq Control Software (MCS), and describe key metrics in Sequencing Analysis Viewer (SAV).

After completing this course you will have an understanding of how the MiSeq processes images to extract intensities and processes intensities to extract base calls. These processes are completed automatically by the MiSeq.

By the end of this course, you will be able to list the key components of the MiniSeq System, describe the lab environment required to install and use the MiniSeq System, and identify the user-supplied consumables required for running and maintaining the instrument.

This course provides an introduction to the Local Run Manager for MiniSeq. Topics include: its purpose, how to access the software, and the analysis workflows. It also shows the steps to set up a sequencing run and view analysis results.

This course provides the basics on how to get started using Sequencing Analysis Viewer (SAV). By the end of this course, you will be able to: identify the purpose of SAV, list the steps to load data into SAV, and describe the tabs in SAV.

After completing this course you will be able to describe the items necessary to run Nextera DNA sample preparation assay to generate MiSeq sequence-ready libraries, describe the steps in Nextera DNA sample preparation workflow, and identify some of the assay's most important considerations.

After completing this course, you will be able to identify the Nextera Rapid Capture Enrichment kits, choose your Nextera Rapid Capture Enrichment Product, identify how the protocol works, describe what is needed to successfully complete the protocol, define the various steps in the Sample Preparation workflow, and analyze Nextera Capture Enrichment data.

After completing this course you will be able to determine appropriate starting material, perform critical techniques used in the sample prep process, and accurately validate the final libraries. Note that the best practices presented in this course do not apply to the Nextera™ DNA Flex Library Prep Kit.

By the end of this training, you will be able to describe the items necessary to run the TruSeq Amplicon Cancer Panel assay, describe the steps in the sequencing workflow, identify some of the best practices, and set up a sample sheet for sequencing.

After completing this course you will be able to describe the TruSeq Targeted RNA Expression Kit, the steps to order a custom oligo pool, the steps in the TruSeq Targeted RNA Expression protocol, best practices, and how to analyze and normalize data.

After completing this course you will understand best practices for all TruSeq Sample Preparation Kits including liquid handling, AMPure XP handling, avoiding cross-contamination, and temperature considerations. You will also learn about specific information about DNA and RNA best practices.

After completing this course you will know the similarities and differences between currently available TruSeq DNA Sample Prep Kits, the starting input amounts and final library characteristics for the various kits, how the various protocols differ, and the ordering information for the various TruSeq DNA kits.

Topics included in this course include the TruSeq DNA PCR-Free library prep protocol, what the protocol does, how the protocol works, what is needed to complete the protocol successfully, and the various steps in the workflow.

After completing this course, you will know: what the TruSeq Nano DNA Sample Prep Protocol is, what the protocol does, how the protocol works, what is needed to successfully complete the protocol, and the various steps in the sample prep workflow.

By the end of this course you will learn what the TruSeq RNA Access Library Prep protocol is, how the protocol works, what is needed to successfully complete the protocol, the various steps in the sample preparation workflow, and how to analyze TruSeq RNA Access Library Prep data.

After completing this course you will understand what sample purification beads are, how the beads work, when the beads are used, what the size selection workflow is, and be able to list some best practices.

By the end of this training, you will be able to define the TruSight One Sequencing Panel, understand how the protocol works, identify what is needed to successfully complete the protocol, describe the various steps in the TruSight One workflow, and identify the tools used for analysis in TruSight One data.

By the end of this course, you will be able to identify the TruSeq Exome kits, choose your TruSeq Exome product, identify how the protocol works, describe what is needed to complete the protocol, define the various steps in the library preparation and enrichment workflow, and analyze TruSeq Exome data.

This course provides an overview of the TruSeq Rapid Exome library prep kit, including, how to choose a TruSeq Rapid Exome product, how the protocol works, what is needed to successfully complete the protocol, what are the various steps in the library preparation workflow and how to analyze TruSeq Rapid Exome data.

By the end of this course, you will be able to identify the Nextera XT DNA kits, identify how the protocol works, describe what is needed to complete the protocol, and define the various steps in the library preparation workflow.