应用

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用

Ab评论

说明

IHC-P

Use a concentration of 4 - 8 µg/ml.

WB

Use a concentration of 1 µg/ml. Predicted molecular weight: 78 kDa.Can be blocked with NRG3 peptide (ab185897). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

功能

Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes.

组织特异性

Highly expressed in most regions of the brain with the exception of corpus callosum. Expressed at lower level in testis. Not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes.

序列相似性

Belongs to the neuregulin family.Contains 1 EGF-like domain.

发展阶段

Isoform 3 is expressed in fetal brain but not in other fetal tissues.

结构域

The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization.ERBB receptor binding is elicited entirely by the EGF-like domain.

翻译后修饰

Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.Extensive glycosylation precedes the proteolytic cleavage (By similarity). Isoform 3 is glycosylated.

细胞定位

Secreted; Cell membrane. Does not seem to be active and Cell membrane. Isoform 3 is also proteolytically released as a soluble form.