Escherichia coli is usually a benign commensal of the gut microflora. However, when E. coli acquires virulence genes it can multiply rapidly and cause disease through colonisation of the intestinal mucosa. Escherichia coli can become a significant pathogen in young pigs. We report an investigation of fatal colisepticaemia in weanling pigs from emerging farms where piglets and weaners were diarrhoeic and the mortality rate ranged between 15% and 70% in each litter. Faecal and tissue samples were processed for histopathology, bacteriology and molecular biology (multiplex and monoplex polymerase chain reaction) and we recovered enteroaggregative multidrug-resistant E. coli producing EAST-1 enterotoxin. An association between poor housing conditions and the observed cases was established and future management programmes were recommended to reduce the impact of such pathogens. Enteroaggregative E. coli is becoming a major problem in the pig industry. It therefore becomes necessary to establish the full impact of E. coli on the South African pig industry and to determine the geographic extent of the problem.

Recent reports have indicated that prevalence and isolation of antimicrobial-resistant E. coli are on the increase (Enne et al. 2008; Luppi et al. 2015; Toledo et al. 2012). Post-weaning diarrhoea (PWD) is a recurrent problem in weaned pigs (3-4 weeks of age) and previous studies have associated this condition with the F4 (mainly K88ac) and F18 fimbriae virulence factors (Nagy & Fekete 1999; Vu-Khac et al. 2006). In South Africa specifically, Henton and Engelbrecht (1997) have serotyped 674 isolates from pigs and found that types O149, O141, O9, O20, O8 and a few others are prevalent in South Africa (arranged here in descending order of prevalence). F4 was associated with 46.9% of the isolates. Similar reports have been obtained from other smallholder farms and emerging pig farms despite slight variations in the pattern of presentation.

In December 2013, carcasses of two five-week-old Large White-Landrace cross weanlings were presented to the Pathology Laboratory of the Faculty of Veterinary Science, University of Pretoria (accession number S04444-13). Earlier, a report based on routine visits of the Porcine Herd Health team to the farm of origin (a start-up farm with 60 sow units) indicated that certain design and management errors had predisposed piglets in the farrowing unit to wet floors, cold draughts and hypothermia and the associated consequences. Suggestions for correction were offered. By the time the carcasses were presented for post-mortem examination, 14 sows had produced an average of 12 piglets per litter, of which 11 per litter were weaned at 4 weeks (weaning weight a 6.7 kg; n = 151). PWD and mortality started 3-7 days post weaning and continued for the next 2 weeks, ultimately becoming self-limiting. Approximately 80% of the piglets experienced PWD to some extent. The mortality rate varied from 15% to 70% in affected litters.

Multifocal villus crypts filled with a monopopulation of small bacterial rods histologically typical of E. coli were observed in tissue samples collected for histopathology (Figure 1a-c). The rod-like organisms adhered to enterocyte brush borders and were scattered within the lamina propria, extending to the lamina muscularis of the small intestine. The intestinal mucosa was not well differentiated and goblet cells were sparse, with a high mitotic rate (Figure 1d), indicative of regeneration and repair.

Multifocal atelectasis was observed in the lungs, interspersed by areas of marked interstitial pneumonia. Large numbers of mononuclear cells, mainly macrophages and lymphocytes, had infiltrated and distended the alveolar walls (Figure 1e-h). Multifocal areas of protein-rich alveolar oedema were observed. The lymph nodes showed numerous lymphoblasts within follicles, with prominent medullary congestion.

Based on the presumptive aetiological diagnosis, diarrhoeic faeces were collected from sick piglets during a follow-up visit to the farm. Culture on blood agar revealed both rough and smooth non-haemolytic E. coli organisms (pathology laboratory accession number B04025-14). During an in-vitro antimicrobial sensitivity evaluation, these organisms were found to be resistant to ampicillin, kanamycin, trimethoprim-sulphadimidine, oxytetracycline and tylosin, but susceptible to cetriofur, enrofloxacin and florfenicol. Subsequent faecal samples collected between January 2014 and June 2015 from this farm and others with matching production statuses revealed similar patterns of clinical signs, pathology and bacterial cultures. However, variable patterns of antibiotic resistance were observed, based primarily on the predominant antibiotics used on the farms.

All isolates were subcultured on blood agar and molecular characterisation was conducted. For this purpose, we conducted an investigation into the virulence factors associated with PWD using standardised protocols for polymerase chain reaction (PCR) and previously determined primers (Table 1).

Escherichia coli DNA was extracted using cell lysis. Bacterial cells were boiled at 99 °C for 15 min, followed by centrifugation. The supernatant containing crude DNA extracts was used in multiplex and monoplex PCR reactions targeting the following virulence factors: heat-labile toxin (LT), heat-stable toxin A (STa), heat-stable toxin B (STb), shiga toxins Stxl, Stx2 and Stx2e, enteroaggregative heat-stable enterotoxin (EAST-1), adhesin involved in diffuse adherence 1 (AIDA-1), porcine attaching- and effacing-associated factor (PAA) and fimbriae F4, F5, F6, F41 and F18. Primers used in the multiplex and monoplex PCR reactions were combined as described by Mohlatlole et al. (2013). The primers that were used for DNA amplification in the PCR reactions are listed in Table 1. The composition of the 25-μL PCR reactions was as follows: 2.5 μM of each primers, 5 μL crude DNA extract, 12.5 μL PCR Master Mix (Fermentas) and sterile DNAse-free water (Fermentas). The conditions for amplification were as follows: 10 min initial denaturation at 94 °C, followed by 30 cycles of denaturation at 94 °C for 30 s, annealing at 56 °C for 30 s and extension at 72 °C for 1 min. To ensure complete amplification of the PCR products, the thermocycling conditions included 7 min of extension at 72 °C.

Monoplex PCR reactions were set up to confirm EAST toxins in the samples. Similar PCR amplification conditions were used, except for the volume of water being adjusted to a final volume of 25 μL using sterile DNAse-free water.

Reference and control samples were included for all PCR reactions. For this purpose, reference samples that are known to be positive for enterotoxins and fimbriae were obtained from the Culture Collection of the Bacteriology Section of the Agricultural Research Council - Onderstepoort Veterinary Institute (ARC-OVI). The strains included B41 (F5:F41:STa), 1883-1 (F18:STa), 1883-2 (F41:F5:STa), 1883-3 (F41:F5:F6:STa), 1883-4 (F4:F5:LT:STb), 1474 K12-K99 Pienk (F5:STa), 1474 K12-K99 Geel (F5:STa) and K99 (F5:STa). In addition, isolates that were known to be positive for EAST-1, PAA, AIDA-1 and E. coli attaching and effacing (EAE) factor reference samples were included. E. coli ATCC 25922 was used as the negative control for virulence factors.

The PCR results indicated that of the 15 virulence factors tested, only EAST-1 yielded a positive band. Although most outbreaks of E. coli-derived diarrhoea in animals have been associated with strains with more than one virulence factor, in this case only EAST-1 toxin was observed (both E. coli isolates were positive for the 125-base-pair toxin; see Figure 2). EAST toxins, produced by enteroaggregative E. coli, are antigenically related to the heat-stable toxins produced by enterotoxigenic E. coli and are known to induce fluid secretion (Veilleux & Dubreuil 2006). Nevertheless, the products encoded by EAST have also been found to be both diarrhoeagenic and non-pathogenic (Ruan et al. 2012). Although our results confirmed that E. coli was involved in causing disease and the associated mortalities in this case, involvement of other organisms was not explored. The EAST-1 toxins have been found in South African pigs in a previous study (Mohlatlole et al. 2013), which reported that 22.5% of the E. coli isolates carried the EAST-1 gene. Similar results have been obtained in studies from Korea and the Czech Republic, although higher prevalences were obtained in sucklers compared with weaner pigs (Lee et al. 2008; Zajacova, Konstantinova & Alexa 2012).

As the EAST-1 toxin could exacerbate the pathogenesis of E. coli diarrhoea in weaned pigs (Choi et al. 2001), it will be necessary to determine the spatio-temporal extent of these organisms in the South African pig population to understand the epidemiology of the infection in the country. Furthermore, no E. coli vaccine available in South Africa confers protection against the EAST-1 toxin, which may partially explain the high mortality observed in some of the cases.

Based on the outcome of the pathology and PCR results, a conclusive diagnosis of colisepticaemia was made. At weaning, piglets are subjected to a variety of stress factors, including the withdrawal of the dam and sow's milk, a dip in the concentration of CD4+ and CD8+ cells, neutrophils and lymphocytes, and a post-weaning syndrome that consists of PWD, oedema disease and endotoxin shock (Almond & Kirk 2010; Van Beers-Schreurs et al. 1992). The change in gut microflora associated with the changing diet around the time of weaning may lead to E. coli overgrowth and subsequent enteritis and endotoxaemia. On this farm, several cases of diarrhoea were observed in the farrowing and weaner pens and respiratory distress was observed in young weaners. Although the E. coli-associated enteritis could have compromised the integrity of the intestinal villi and reduced the efficiency of feed utilisation, the cause of death in these weaner pigs was E. coli-associated endotoxaemia. No cases of piglet anaemia were observed in the herd and the oxygen-carrying capacity of haemoglobin did not appear compromised. The consolidation and interstitial alveolar wall thickening observed in the lungs notably reduced the capacity of the lungs in complying with their primary role of oxygen-carbon dioxide exchange at the alveolar level. Fast-growing weaner pigs need an increasing level of such gaseous exchange to meet their physiologic needs at this stage. It becomes vitally important to reduce other associated complications (e.g. digestive or respiratory) arising from E. coli infection at the time of weaning.

We recommended changes in management protocol at this farm to ensure dry floors and hygiene in the farrowing and weaner units. Establishment of a vaccination protocol that includes routine E. coli vaccination of sows 2 weeks before parturition is also recommended to boost colostral immunity and decrease the magnitude of antibiotic treatment in subsequent outbreaks.

Acknowledgements

We thank the staff of the Pathology Section of the Department of Paraclinical Sciences, the Microbiology Laboratory of the Department of Veterinary Tropical Diseases and the Porcine Herd Health students (PHP 650) of the Faculty of Veterinary Science, University of Pretoria, who all participated in the investigation. We also appreciate the cooperation of the farmer. The molecular biology work was carried out at the ARC-OVI, Onderstepoort, South Africa.

Competing interests

The authors declare that they have no financial or personal relationships that may have inappropriately influenced them in writing this article.

Authors' contributions

F.O.F. (University of Pretoria) and D.G.B. (University of Pretoria) drafted and revised the manuscript. F.O.F. attended to the case and performed clinical sampling and follow-up. E.M. (ARC-OVI) was responsible for bacteriology work. All authors contributed to the writing of the manuscript and approved it for submission.