* The nanoparticles produced from this reaction will be used in the silica coating procedure in the future. We are interested in finding a procedure for synthesizing AuNP that has a low diameter variation. The current procedure has a wide range (5-40nm) that greatly affects the amount of PVP/TES that need to be added for the silica coating procedure. Minimizing changes in variables will help simplify the procedure.

Current revision

Preparation of DNA/MB Samples for FCS

Prepare the following samples:

1) 50nM MB + 40nM DNA

2) 10nM MB + 8nM DNA

3) 5nM MB + 4nM DNA

4) 500pM MB + 400pM DNA

5) 100pM MB + 80pM DNA

6) 50pM MB + 40pM DNA

7) 20pM MB + 16pM DNA

13.7nm AuNP synthesis

The nanoparticles produced from this reaction will be used in the silica coating procedure in the future. We are interested in finding a procedure for synthesizing AuNP that has a low diameter variation. The current procedure has a wide range (5-40nm) that greatly affects the amount of PVP/TES that need to be added for the silica coating procedure. Minimizing changes in variables will help simplify the procedure.