IB research on the efficiency of oocyte vitrification: open system V closed system.

12-05-2016

Vitrification almost entirely eliminates the most significant issue in freezing which is the formation of ice crystals. Carrying it out implies high concentrations of cryoprotectants and extremely fast cooling. One of the means of achieving such elevated cooling rates consists of immersing the sample directly in liquid nitrogen using open systems.

However, there is a fear of the potential risk of sample contamination through the particles in the nitrogen when open systems of this kind are used. The immediate answer lies in the use of closed systems that isolate the sample and avoid direct contact with the nitrogen, taking into account that this can have a negative impact on optimum cooling rates. This is particularly relevant in oocytes since, because of their characteristics, they do not respond well to cryogenic temperatures. Recent work has shown that warming speeds when defrosting are, in fact, more important than cooling rates.

In line with our data, the use of the closed system does not affect oocyte survival and results are similar to those obtained with open systems. Additionally, the risk of contamination is eliminated. Whilst there have been no confirmed cases of contamination on a global scale in the field of reproductive medicine, it is expected that future European regulations will call for the use of closed systems that guarantee asepsis.

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