DNA migration problem - Genomic DNA (Aug/07/2008 )

dear all, Last day after I prepared genomic dna , I set up pcr with previously used primerz and I found there was no amplification but there was proper band of g-DNA just below the wells ...do you think excess DNA will inhibit PCR? will remaining phenol in G-DNA prep inhibit PCR?does remaining phenol in G-DNA prep after PCI also inhibit DNA solubility even after EtOh step?(I found the DNA to be a bit insoluble after drying)can G-DNA (excess) trap PCR products and inhibit migration in Gel?please respond.Regards..Srijit

-srijitkhan-

what was the quantity of the reagents and concentration, Tm of the primers, the program of thermocycler and if possible attach a photo of the gel.