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Qiagen produces an excellent range of products for the isolation and purification of nucleic acids in various contexts. Recently, they have added to this line-up with the release of the HiSpeed ... read more

There are a number of different products available to perform transfections, yet the efficiency of molecule delivery into the cell varies with each transfection protocol and is often cell-line ... read more

The QIAexpress System contains the vectors, bacterial strains, affinity matrix and antibodies required for the purification of 6x-his tagged proteins. The QIAexpress System is made up of four ... read more

The QIAquick Gel Extraction Kit can be used after many manipulations (restriction digests, PCR, phosphatasing) to rapidly 'clean-up' DNA for downstream applications (ligations, in vitro transcription,... read more

Some time ago I came across Qiagen’s OmniScript Reverse Transcriptase system to prepare CyDye labeled cDNA from total RNA (or mRNA) for microarray hybridization. Since then, I have used this ... read more

The RNeasy kit can purify total RNA from animal cells or tissues, bacteria and yeast and another similar kit is available for the extraction of total RNA from plants and filamentous fungi, the RNeasy ... read more

The TransMessenger Transfection Reagent from Qiagen is designed for the efficient transfection of eukaryotic cells with RNA (including siRNA). The reagent works using principles similar to the ... read more

Today, it seems like every company on the market sells a plasmid miniprep kit, thus, researchers have several to choose from. Our lab is currently using Qiagen’s QIAprep miniprep kit as our miniprep ... read more

Every molecular biologist is concerned about the purity of their DNA samples. Whether performing RT-PCR, nested PCR, sequencing or fragment analysis, a critical step for a successful outcome is the ... read more

This kit from QIAGEN allows you to go from bacterial culture to reasonably pure plasmid in about 20 minutes using a simple lysis, followed by elution from a supplied bind-and-release spin column. read more

Submitted by:Boyd ScottSenior Research Scientist/Vitae PharmaceuticalsQiagen’s QIAprep Miniprep Kit is based on alkaline lysis of bacteria followed by adsorption onto a silica-gel-membrane in the ... read more

Pure plasmid DNA is a critical component to success in any molecular biology lab as the majority of follow-up applications, from sequencing to transfection, relies on uncontaminated DNA. The Qiagen ... read more

All of Qiagen’s plasmid purification kits are based on a modified alkaline lysis protocol, followed by the binding of DNA to an anion-exchange resin at low salt and pH conditions. Impurities are ... read more