Bioactives, Inc.

Delivery Systems

Aquosol, water-soluble resveratrol

The solubility of the powdered trans-resveratrol in pH 7.4 Hank's Balanced Salt Solution (10 mM HEPES and 15 mM glucose) was compared to the water-soluble trans- resveratrol formulation (Aquosol™). At least 1 mg of powdered, synthetic trans-resveratrol extract or 100 mg of water-soluble resveratrol formulation, made with the same resveratrol, was combined with 1 ml of buffer to make a ³1 mg/ml powdered resveratrol extract mixture and a ³1 mg/ml resveratrol water-soluble formulation mixture, respectively. The mixtures were shaken for 2 hours using a bench top vortexer and left to stand overnight at room temperature. After vortexing and standing overnight, the powdered resveratrol extract mixture was then filtered through a 0.45-mm nylon syringe filter (Whatman, Cat# 6789-0404) that was first saturated with the sample.

After vortexing and standing overnight, the water soluble resveratrol formulation mixture was centrifuged at 14,000 rpm for 10 minutes. The filtrate or supernatant was sampled twice, consecutively, and diluted 10, 100, and 10,000-fold in a mixture of 50:50 assay buffer:acetonitrile prior to analysis.

Both mixtures were assayed by LC/MS/MS using electrospray ionization against the standards prepared in a mixture of 50:50 assay buffer:acetonitrile. Standard concentrations ranged from 1.0 mM down to 3.0 nM. Results are presented in Table 1 below.