Overview

SpecificityThis antibody, also known as the estrogen regulated 24 kD protein and HSP 28, is one of the several heat shock proteins (HSP) produced by all organisms studied. The antigen is induced by elevated temperature as well as estrogen in hormone response. This protein has been studied in human estrogen response tissues such as cervix, endometrium and breast tissue. It may be useful in classification of various hormone sensitive tumors.

Involvement in diseaseDefects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.

Sequence similaritiesBelongs to the small heat shock protein (HSP20) family.

Thank you for your enquiry.
Hsp27 is cytoplasmic in interphase cells, colocalizes with mitotic spindles in mitotic cells and translocates to the nucleus during heat shock. I would recommend that your customer uses a RIPA buffer extraction.
I ho...

Thank you for your enquiry.
I am sorry to hear that your customer has been having difficulties with this antibody.
I have read your customer's questionaire and they are following a protocol that I would largely recommend. However, I would appre...

We do not know for sure whether this anitbody recognises phosphorylated and non-phosphorylated forms of HSP27. It might be possible to find the answer in published work involving this antibody - there are five listed reference on the datasheet. We do ...