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2015 JALA & JBS Art of Science Contest Winners

Grand Prize Winner

Gary James Sarkis, PhD, of GE Healthcare, Piscataway, NJ (USA)

The Bee's Knees: Hidden detail and beauty, in his daughter's beginners microscopy set, is seen when a bee's leg is imaged in green, yellow and orange fluorescent light at 4X magnification on the Cytell Cell Imaging System

“Electric skies” in an image of transgenic mouse primary cortical neurons stained for markers Tau (red-orange), Neun (yellow) and nuclei (blue). The images was taken on an older Opera system, 40x water objective and later processed for image analysis. The overall effect in the image is kinetic and electrical. Several colleagues said the image reminded them of Van der Graaf generators; one said it reminded her of the Sistine Chapel, where the two fingers of human Earth and Heavenly God try to touch.

Hong Chen (USA)

It shows eight fluid diodes, made on layered paper, are grouped to four fluid circuits. Each circuit consists of two oppositely configured diodes, a shared fluid inlet, and two outlets. Image are taken after four drops of dyed fluids are added to each inlet of the circuits. The wetting patterns shows the fluid diode only allows fluid flow in one direction in porous materials (like paper).

John Holleran (Australia)

Glamorous Gametocytes: This image shows the transmission forms of malaria parasites called gametocytes (green) inside human red blood cells (red). Gametocytes develop within red blood cells over a 2 week period and are sequestered in the bone marrow. At the end of development, gametocytes adopt this crescent shape in order to avoid clearance by the spleen and are released into the blood stream. A mosquito feeding on an infected host will pick up the gametocytes and continue the transmission cycle. This image was obtained using a confocal fluorescence microscope (Perkin Elmer Opera) using 40X magnification. Gametocytes were detected by GFP fluorescence and red blood cells were stained using a lipophillic membrane dye (Cell Mask PM orange). This image represents the output for a high-content image-based screening approach we are using to identify new molecules which can change the deformability of gametocytes. Compounds which increase the retention of gametocytes in the spleen will remove them from the blood circulation and eliminate them from the transmission cycle.

Peng Li (China)

Live MCF-7 cell, 60umx60um, SICM, standing approach mode.

Gary Sarkis 2 (USA)

A cross section of H&E stained Lingual papillae filiformes was imaged at 4X magnification in Green, Red, and Blue fluorescent light on the Cytell Cell Imaging System. The images were fused to create the tasteful image.

Phospholipid Bilayer Liposome Model: Visualization of a cellular membrane vesicle, or liposome, used in a model to study nanoparticle toxicity. Included in the model are phospholipids, cholesterol, and membrane proteins. Image rendered using Autodesk 3D Studio Max.

Yu Shrike Zhang (USA)

The Stunning Uniformity: Three-dimensional porous scaffolds play a critical role in engineering functional tissues that can potentially replace those damaged by diseases or injuries. As a new class of scaffolds, the "inverse opal scaffolds" are uniquely featured by their extraordinarily uniform pores and well-ordered "honeycomb" arrangement, which can greatly facilitate the creation of functional tissue substitutes by allowing efficient movement and growth of cells inside the scaffolds. This microscopic image shows an inverse opal scaffold made from a biodegradable polymer, with a uniform pore size of 200 micrometers (about the size of two human hairs). The image is coded in different colors by depth, with blue showing the surface and red the bottom.

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