This is used for lysing yeast at pH6.8. For pH8 lyses, use the variation of SUMEB with Tris buffer known as SUTEB.
Also, bromophenol blue can be left out if dye is not desired, such as using the lysate for immunopreicipations.

50X STOCK Protease Inhibitors (store at -20oC)

PMSF (87 mg/ml)
([500mM] phenylmethylsulfonyl fluoride)

TO MAKE 30 ml: Dissolve 2.61g PMSF in DMSO to equal a
final volume of 30 ml.

LEUPEPTIN AND PEPSTATIN (5 mg/ml)

TO MAKE 10 ml: Dissolve 50mg LEUPEPTIN or PEPSTATIN in DMSO to equal a
final volume of 10 ml.

TPCK (5 mg/ml)(tosylphenylalanine chloromethyl ketone)

TO MAKE 10 ml: Dissolve 50mg TPCK in DMSO to equal a
final volume of 10 ml.