Introducing Sierra SPR-24 Pro - High Performance, High Throughput SPR

From screening and kinetics, to epitope binning and thermodynamics, Sierra SPR-24 Pro provides industry leading SPR performance and analysis throughput, across the widest range of applications.

24 Sensor SPR Detection

The Sierra SPR-24 Pro system employs a 24 sensor detection array laid out in 8 flow cells, each containing 3 sensors. Up to 8 samples can be analyzed simultaneously, generating up to 24 binding measurements per analysis cycle. All 24 sensors can also be addressed fully independent of the others, providing maximum assay design flexibility.

Throughput with Performance

Capable of testing one sample at a time, the Sierra SPR-24 Pro system really shines in high throughput applications where it can process 8 samples simultaneously over 24 sensors. Utilizing Bruker’s Hydrodynamic Isolation™ (HI) continuous flow microfluidic technology to address samples as flowing streams onto the 24 spot SPR+ sensor array. When integrated with an optional plate handling robot, the Sierra SPR-24 Pro instrument can assay over 4400+ samples per day, generating over 8800+ control subtracted binding responses.

Throughput for all Applications

From screening assays with a different target on each sensor, to the detailed kinetic analysis employing multiple in-line controls, throughput improvements can be realized for all SPR applications.

Better Data Faster

This three-sensor per channel design provides maximum control analysis flexibility, enabling high throughput for even the most complex analyses. Samples can be simultaneously assayed on two controls as well as the active sensor.

In many SPR applications the early analysis of non-specific binding to matrix proteins such as HSA or BSA can be critical. The additional in-line control not only halves the number of assay cycles required vs. two sensor flow cells. It can also improve data quality by eliminating potential variations between multiple sample preparations.

Technical Details

Analytical Biosensor for the Real-Time, Label-Free Characterization of Molecular Interactions

Continuous Flow SPR Analysis

The SPR-24 Pro system is a continuous flow biosensor, meaning sample and reagents are delivered to the detection sensors as continuously flowing streams. When sample or reagent is not being injected “continuous flow buffer” is passed over the sensors.

The formulation of the continuous flow buffer in SPR assays can have significant impact on the molecular interactions being measured. In many applications measuring target interactions in a variety of buffer environments are required.

Frame Inject

The Frame Inject feature in the Sierra SPR-24 Pro system allows the investigation of condition dependent binding / mode of action studies. The feature requires just a fraction of each buffer condition, that replaces the system buffer while usage. The pre-association, as well as the dissociation occurs under the conditions, that should be investigated. After dissociation end, frame solution buffer is replaced and system buffer is flowingthrough the microfluidic system again. Thus experimental time and reagent costs are kept low. The same buffer can be directed to all flow cells, or up to eight different buffers can be assayed simultaneously.

Frame Inject allows the investigation of up to eight buffer conditions in parallel.

Individual Needle Control (INC)

The Individual Needle Control (INC) feature in the Sierra SPR-24 Pro system allows the eight sample pickup needles to be operated independently as well as in unison. Researchers can choose any combination from 1 – 8 needles to design their optimal assay.

Maximizing Performance with INC

Individual Needle Control maximizes the performance, flexibility, and scope of applications possible with the Sierra SPR-24 Pro instrument, while also saving time, materials, and resources when throughput is not the main requirement.

INC Analysis

The eight-needle probe head can simultaneously process 8 sample or reagent solutions. Using the INC feature, users can choose to use any number and/or combination of needles at any time during analysis.