Vitalii Pervushyn was born in 1974 in Nemirov of the Vinnitsa region of Ukraine in a family of workers. After finishing first in average school, in 1989 he entered the Vinnitsa Musical College named of Leontovich in the violin class of Professor Kalinovsky. Three years later he entered the Kyiv National Conservatory named Tchaikovsky in the violin class, continuing with postgraduate study after 1997.

From 1995 to 2000 Vitalii played in the National Philharmonic Orchestra of Ukraine as well as the Chamber Orchestra Kyiv Soloists headed by Professor Kotorovich. In 2000 he won the Davidoff Grant and became subprincipal in the Philharmonia of the Nations, headed by Justus Franz. In 2008 Pervushyn was appointed to the Qatar Philharmonic Orchestra as sub-principal violin.