DESKGEN Activate Library

Activate gene expression with CRISPRa.

Monitor the role of unknown DNA elements

DESKGEN Activate Libraries use CRISPRa to enable multiplex activation of genes as well as non-coding genomic elements.

They are ideally suited for understanding the function of noncoding RNAs, identifying drug resistance pathways, fluorescently imaging proteins and more.

Designed for maximum impact by DESKGEN AI

Activate Libraries are designed to target in optimal proximity to transcription start site (TSS), selected using our proprietary omics pipelines. This process provides the maximum activation effect for clean and robust data.

Customize your Activate Library

Parameter

Customizable?

Example

A Case Study

How researchers used our CRISPRa libraries to activate gene transcription

Understanding our client

A researcher from NYU School of Medicine was interested in executing a CRISPRa screen to interrogate the function of multiple genomic elements. To accomplish this goal, the researcher needed to target specific genomic loci unavailable in traditional gene panels offered by other library providers.

Made-to-order library specifications

Our client wanted to target noncoding DNA. Therefore, the guide RNA library needed to target specific uncommon coordinates in the genome. Our bioinformaticians took this information and used DESKGEN AI to design a robust guide list for the client, and these designs were then synthesized as oligos.

DESKGEN Activate Library

Parameter

Approach

Library type

Pooled

Nuclease

dCas9

Reference genome

Human (GRCh38)

Activator construct

VP64

Vector system

Two-vector

Selection marker

GFP

Target frame

-1 to -200 bp from TSS

Strand constraints

Exclude BsmBI site

Full documentation

To support further investigation, we provided our client with a final DESKGEN Library Manifest which detailed the designs of all guides and their specifications, including thresholds for activity, specificity and other parameters.