Cystatins are a superfamily of cysteine proteinase inhibitors found in both plants and animals. They comprise a group of proteinase inhibitors, widely distributed in tissues and body fluids, and form tight complexes with cysteine proteases such as cathepsin B, H, L and S. Cystatin C, a secreted molecule of this family, is of interest from biochemical, medical and evolutionary points of view. Cystatin C, with molecular weight of 13,260D, is composed of 146 amino acids, lacks carbohydrate and has two disulfide bridges located near the carboxyl terminus. Cystatin C is increased in patients with malignant diseases and is related to the insufficiency of renal function and appears to be a better marker than creatinine. On the other hand, low levels of cystatin C involve cause the breakdown of the elastic laminae and, subsequently, the atherosclerosis and abdominal aortic aneurysm.

Catalog #

C8950-04

Applications

Suitable for use in ELISA. Other applications have not been tested.

Recommended Dilutions:

Optimal dilutions to be determined by the researcher.

Matched Pairs

Capture: C8950-04

Detection: C8950-09, C8950-06, C8950-10B

Hybridoma

Sp2/0 myeloma I cells with spleen cells from Balb/c mice

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Clone Type

Monoclonal

Isotype

IgG1

Clone No

8J181

Host

Mouse

Source

Human

Concentration

As Reported

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide

Purity

Purified by Protein A afffinity chromatography. Purity is tested by electrophoresis(.>90% pure.)

Immunogen

Cystatin C purified from human urine

Specificity

Recognizes human Cystatin C.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.