Akway (Drimys piperita Hook.f.) is a medical plant used by Sougb Tribe, Arfak Mountains Regency, West Papua Province to heal malaria. Previous researches indicated that medical plants had strong antibacterial properties. The objectives of the research were to evaluate in vitro antibacterial potency of ethylacetate extracts of akway barks and its applications as preservative agent on meatballs during room temperature and cold storage. In vitro antibacterial assays were done on concentration of 0-25% (w/v) using agar well diffusion method while antibacterial assays on meatballs on concentration of 0-0.75% (w/v) were performed using total plate counts methods. Results showed that ethylacetate extracts inhibited in vitro growth of Escherichia coli ATCC25922, Bacillus cereus ATCC10876, Pseudomonas aeruginosa ATCC27853 and Staphylococcus aureus ATCC25923 with MIC of 0.27-0.77% (w/v). Concentration of extracts significantly affected the growth of bacteria. The increasing of extract concentrations result in increasing diameters of growth inhibition zones. Meatballs soaked in extract solutions with concentrations of 0.25-0.75% (w/v) inhibited growth of bacteria during 3-8 days in room temperature storage and 15-33 days during cold storage in refrigerator. As conclusion, ethylacetate extracts of akway barks had high potent use as preservative agent in food model of meatballs.