Subject: Watching in the inner life of a cell: beyond super-resolution microscopy

Cellular processes are orchestrated by a large number of biomolecules in a spatially and temporally coordinated manner within a tiny volume. To uncover the underlying organizational principles and their functional relevance, we take microscopy visualization as the primary approach to map the spatial localization, temporal dynamics and activity profiles of proteins and nucleic acids. We have used super-resolution microcopy to dissed the molecular organization of the cilium transition zone, revealing its function as a gate of ciliary trafficking. We have also been developing techniques for the mapping of the physical organization of the genome in the nucleus.