Background The physiology of water polo has been difficult to study due to the characteristics of the game in the field of play. Little is known about cardiac electrophysiology in these athletes, particularly in women. The electrocardiographic variables in elite water polo athletes have been rarely studied. Objectives To analyze ventricular repolarization in female water polo players from the National Pre-selection Team of Cuba Methods A cross-sectional study was performed in two groups of women, one with 20 athletes and another with 20 healthy nonathletes. The electrocardiographic parameters of ventricular repolarization were compared between both groups. Results Mean age was 22.45±5.30 years vs. 22.45±5.30 years; p >0.05. Weight was greater in athletes compared to nonathletes (69.63±7.73 kg vs. 51.20±6.63 kg; p <0.001). Mean duration of sporting practice was 13.5 years. There were no significant differences in QTc and JTc intervals in lead DII between both groups, yet the intervals measured in lead V5 were longer in athletes (QTc: 405 ms vs. 424 ms; p <0.05 - JTc: 307 ms vs. 329 ms; p <0.05). QT interval dispersion was greater in athletes compared to non-athletes (74 ms vs. 43 ms; p <0.001). Tpeak-Tend interval was greater in athletes in leads V1 (64 ms vs. 81 ms; p <0.05), V3 (78 ms vs. 92 ms; p< 0.05), V4 (72 ms vs. 83 ms; p <0.05) and V5 (63 ms vs. 80 ms; p <0.05). Conclusions QTc and JTc intervals and QT interval dispersion were significantly greater in water polo athletes. Tpeak-Tend interval was significantly longer in leads V1, V3, V4 and V5 in the athlete group. Finally, although this group showed greater Tpeak- Tend interval dispersion, it was not statistically significant.