I have tried to express some proteins in pGex2T in Ecoli. When I
expressed them in small prep (50 mL), there was some breakdown but not
significant. However, when I expressed them in a larger volume
(300mL), there was a lot of breakdown. I expressed them at 37
degree for only an hour after growing them to higher density. Is
there any chang that I can do to reduce breakdown of my protein? It
is really frustrating.
Thanks