Forum for Science, Industry and Business

Cell on a Chip Reveals Protein Behavior

19.03.2013

In the future, artificial cells may produce complex protein structures on demand

For years, scientists around the world have dreamed of building a complete, functional, artificial cell. Though this vision is still a distant blur on the horizon, many are making progress on various fronts. Prof. Roy Bar-Ziv and his research team in the Weizmann Institute’s Materials and Interfaces Department recently took a significant step in this direction when they created a two-dimensional, cell-like system on a glass chip.

Protein interaction on a chip: Red proteins concentrated more on the right, farther from the chip-bound genes, while green proteins are more highly concentrated on the left, closer to the genes that encode them

Illustrated biological chip: Genes (gray) are attached to the chip, along with antibodies (blue). The gene encoding the second protein is purple. RNA strands (red) are copied, and, depending on the information they encode, the proteins produced in the ribosomes (yellow) glow either green or red (green and purple cylinders)

This system, composed of some of the basic biological molecules found in cells – DNA, RNA, proteins – carried out one of the central functions of a living cell: gene expression, the process by which the information stored in the genes is translated into proteins. More than that, it enabled the scientists, led by research student Yael Heprotein yman, to obtain “snapshots” of this process in nanoscale resolution.

The system, consisting of glass chips that are only 8 nanometers thick, is based on an earlier one designed in Bar-Ziv’s lab by Dr. Shirley Daube and former student Dr. Amnon Buxboim. After being coated in a light-sensitive substance, the chips are irradiated with focused beams of ultraviolet light, which enables the biological molecules to bind to the substance in the irradiated areas.

In this way, the scientists could precisely place DNA molecules encoding a protein marked with a green fluorescent marker in one area of the chip and antibodies that “trap” the colored proteins in an abutting area. When they observed the chips under a fluorescence microscope, the area in which they had placed the antibodies turned a glowing bright green. This meant that the DNA instructions had been copied into RNA molecules, which were in turn translated into fluorescent green proteins. The green proteins were then ensnared by the antibodies.

Next, the scientists asked whether their cell-like system could reproduce complex structural assemblies of naturally-occurring proteins. This time, they attached a viral gene to the chips’ surface encoding a protein that can self-assemble into a nanotube. With the help of Dr. Sharon Wolf of the Electron Microscopy Unit, they observed a forest of minuscule tubes sprouting from the antibody area under an electron microscope.

The researchers then sought a way to produce and trap multiple proteins simultaneously by confining each protein in the area of its gene on the chip. On top of the chip to which the DNA encoding green proteins was bound, the scientists added a solution with a second gene encoding a red protein. The resulting red and green proteins competed for binding on the antibody traps, yielding a graded spatial separation in which the antibodies closest to the green genes had the highest concentration of green protein, with red concentrations rising farther afield. The results of this research recently appeared in Nature Nanotechnology.

Bar-Ziv: “We have shown that it is possible to build a protein 'production line' outside of the cell and use it to observe a spectrum of protein activities.” In the future, such a system may move from enabling the observation of proteins to providing the basis for techniques to create complex, active protein structures on demand.

Prof. Roy Bar Ziv’s research is supported by the Yeda-Sela Center for Basic Research; and the Carolito Stiftung.

Die letzten 5 Focus-News des innovations-reports im Überblick:

Physicists of the University of Würzburg have made an astonishing discovery in a specific type of topological insulators. The effect is due to the structure of the materials used. The researchers have now published their work in the journal Science.

Topological insulators are currently the hot topic in physics according to the newspaper Neue Zürcher Zeitung. Only a few weeks ago, their importance was...

In recent years, lasers with ultrashort pulses (USP) down to the femtosecond range have become established on an industrial scale. They could advance some applications with the much-lauded “cold ablation” – if that meant they would then achieve more throughput. A new generation of process engineering that will address this issue in particular will be discussed at the “4th UKP Workshop – Ultrafast Laser Technology” in April 2017.

Even back in the 1990s, scientists were comparing materials processing with nanosecond, picosecond and femtosesecond pulses. The result was surprising:...

A multi-institutional research collaboration has created a novel approach for fabricating three-dimensional micro-optics through the shape-defined formation of porous silicon (PSi), with broad impacts in integrated optoelectronics, imaging, and photovoltaics.

Working with colleagues at Stanford and The Dow Chemical Company, researchers at the University of Illinois at Urbana-Champaign fabricated 3-D birefringent...

In experiments with magnetic atoms conducted at extremely low temperatures, scientists have demonstrated a unique phase of matter: The atoms form a new type of quantum liquid or quantum droplet state. These so called quantum droplets may preserve their form in absence of external confinement because of quantum effects. The joint team of experimental physicists from Innsbruck and theoretical physicists from Hannover report on their findings in the journal Physical Review X.

“Our Quantum droplets are in the gas phase but they still drop like a rock,” explains experimental physicist Francesca Ferlaino when talking about the...