Novice or expert—learn the basics of fragment analysis

Fragment analysis applications are those in which fluorescent fragments of DNA (produced by PCR using primers designed for a specific interrogation task) are separated using capillary electrophoresis and sized by comparison to a size standard.

Most fragment analysis applications have simplified workflows—straightforward sample preparation (without the purification required for sequencing samples), ability to multiplex, and applications can all be separated using the same capillary electrophoresis (CE) polymer.

The video gives an overview of the fragment analysis workflow and introduces you to some of the applications you can develop on our CE instruments.

Step-by-Step Guide to General Fragment Analysis

DNA extraction is a critical first step in the experimental workflow of Fragment analysis. The overall quality of the fragment analyzed can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction.

During capillary electrophoresis, the products of the PCR are injected electrokinetically into capillaries filled with polymer. High voltage is applied so that the fluorescent DNA fragments are separated by size and are detected by a laser/camera system.

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DNA extraction is a critical first step in the experimental workflow of Fragment analysis. The overall quality of the fragment analyzed can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction.

During capillary electrophoresis, the products of the PCR are injected electrokinetically into capillaries filled with polymer. High voltage is applied so that the fluorescent DNA fragments are separated by size and are detected by a laser/camera system.

Step-by-Step Guide to General Fragment Analysis

DNA extraction is a critical first step in the experimental workflow of Fragment analysis. The overall quality of the fragment analyzed can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction.

During capillary electrophoresis, the products of the PCR are injected electrokinetically into capillaries filled with polymer. High voltage is applied so that the fluorescent DNA fragments are separated by size and are detected by a laser/camera system.

Recommended Products:

DNA extraction is a critical first step in the experimental workflow of Fragment analysis. The overall quality of the fragment analyzed can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction.

During capillary electrophoresis, the products of the PCR are injected electrokinetically into capillaries filled with polymer. High voltage is applied so that the fluorescent DNA fragments are separated by size and are detected by a laser/camera system.