The other genes were not amplified. There are many reasons for why LTP antisense and Bet v1 did not amplify, so we tried to eliminate some potential problems. After checking the primers against the arabadopsis genome, the primers seem correct. Annealing temperatures also fall within the appropriate range.

Other possible problems could be contamination, improper pH, insufficient DNA, primer design beyond sequencing (unlikely), or incorrect primers in a reaction (unlikely). We will obtain more arabadopsis genomic DNA and try PCR again.