This Niemann-Pick C1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections,Western blot. In Western blot the antibody detects heterogeneously glycosylated NPC1 protein with prominent bands at 170 and 220 kDa. Results with mouse in Western blot have been mixed. It has also been tested for immuno-EM (on human protein only). Use in Electron Microscopy reported in scientific literature (PMID: 21051527). Use in Immunocytochemistry/immunofluorescence and western blot reported in scientific literature (PMID:24209575).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Buffer

PBS

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Purity

Immunogen affinity purified

Alternate Names for Niemann-Pick C1 Antibody

FLJ98532

Niemann-Pick C1 protein

Niemann-Pick disease, type C1

NPC

NPC1

Background

Niemann-Pick type C1 (NPC1) is a member of a family of genes encoding membrane-bound proteins containing putative sterol sensing domains. NPC1 protein regulates cholesterol transport from late endosomes-lysosomes to other intracellular compartments. NPC1 overexpression increases the rate of trafficking of low density lipoprotein cholesterol to the endoplasmic reticulum and the rate of delivery of endosomal cholesterol to the plasma membrane. NPC disease is an inherited neurovisceral lipid storage disorder of unesterified cholesterol accumulation in lysosomes. It is characterized by progressive neural and liver degeneration, resulting from inactivating mutations in NPC1, in most cases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Video Protocols

I'm looking for a Niemann-Pick type1 or 2 antibody to stain mouse heart section embedded in paraffin. I would like to label these sections with another primary antibody to show colocalisation of my protein of interest. I would like to know if you have a rabbit anti-Niemann-Pick antibody that works well for fluorescence immunohistochemistry?

Niemann-Pick C1 Antibody (<a href="http://www.novusbio.com/NB400-148" target="_self">NB400-148</a>) (0.1 ml) is one of our best sellers and this antibody has been cited in at least 17 peer reviewed research publications in journals of high repute. We have only one Niemann-Pick type C2 antibody with catalog # <a href="http://www.novusbio.com/H00010577-D01P" target="_self">H00010577-D01P</a>, but this antibody is yet to be established for IHC application. However, if you would like to try this antibody for IHC-P application you would qualify for our Innovators Reward program. Through this program if you complete an online review with image, detailing your positive or negative results we will send you a discount voucher for 100% of the purchase price of the reviewed product. Read more about <a href="http://www.novusbio.com/support/innovators-reward.html" target="_self">Innovators Reward Program</a>.

We have an inquiry of your product Cat. ID # NB400-148. Our customer would like to know the immunogen sequence of this product. If you cannot provide the detail, would you please check if this antibody covers amino acids 600-800?

This antibody was made to a sequence found within amino acids 1230-1278.

Have you received any complaints on these lots F1 and G1 of NB400-148?

I have looked through our complaints and spoke to our lab manager, and we have not had any recent reported problems with these lots. These lots do happen to be approximately two years old, so it may be that they have lost reactivity over this time. Our antibody products are guaranteed for 6 months from date of receipt.

I want to purchase NPC1 antibody from your company and the catalog is NB400-148. Could you please tell me in which precise position the antibody binds of the NPC1 protein?

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