Cutting fixed brains at 20 microns

From:

Nancy Maronto <nmaronto@hotmail.com>

We are asked to cut 20 micron sections of rat brains perfused in a
paraformaldehyde fixative. Can you experts out there suggest ways of doing
this. I know of adding sucrose to tissue and doing frozen sections. Which
may be very difficult. The equipment we have is a tissue tech cryostat and
rotary microtomes. Thanks in advance for your help.
Nancy Maronto
MPI Research
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