3D Localization and Tracking Using the SPINDLE™ with Variable-Angle Illumination Epifluorescence Microscopy

Using the SPINDLE™, Wang et al showed that combining the Double Helix-PSF with variable-angle illumination epifluorescence microscopy (aka pseudo-TIRF), the signal to noise ratio, and hence, the localization precision of point emitters can be improved up to five-fold. This study will allow users of the SPINDLE™ to improve the accuracy of their image and track reconstructions with their current microscope set-up.

APPLIED PHYSICS LETTERS 2017

3D Super-Resolution Uncovers RNA Stress Granule Cores

Jain and Wheeler et al. utilize the SPINDLE™ to visualize and characterize previously unseen stress granule cores. Stress granules are sub-cellular aggregates associated with ALS and Dementia. This ground-breaking study proposes a new model for the formation and dynamics of stress granules based on proteomic studies, in vitro isolation, and in vivo 3D Double Helix super-resolution imaging.

CELL JANUARY 2015

APPLICATION NOTE

Multi-Color 3D Super-Resolution of Live Bacteria

Gahlmann et al. utilize Double Helix with super-resolution to reconstruct three dimensional, three color super-resolution images of live bacteria using fluorescent proteins and a lipophilic dye. This innovative study quantitatively describes the sub-cellular structure of Caulobacter crescentus bacteria.