This assay employs an antibody specific for Human IGF1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGF1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

機能The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.

This kit is designed to measure free IGF1. IGF-1 is usually bound to IGFBPs in the serum/plasma, so if you would like to measure total IGF1, it is advisable to release the analytes by performing an extraction procedure such as acid-ethanol treatment (P...

Unfortunately the immunogen sequence for the antibodies used in the kit ab100545 is not available. Nor do we have cross reactivity data for kangaroo species. If it helps, the capture antibody used is monoclonal and the detection antibody is polyclonal....

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I have been in contact with the lab regarding your inquiry.They are not familiar with the Immulite 1000. However, as long as the system can perform the protocol as written, we do not see why it would not work. As this sy...

I am not too familiar with SST tubes, but we do not recommend collecting the blood into tubes with additives. Please ensure the customer pays close attention to the sample pretreatment steps in the manual and also follows the recommended serum dilution...