The Universal Plant ChIP-seq kit offers the convenience of extracting plant chromatin from a wide variety of plants including Arabidopsis, maize, rice, tomato and poplar. This complete kit has been specifically optimized for plant chromatin extraction and includes reagents for chromatin preparation, immunoprecipitation, plant-specific control primer pairs, control antibody, and DNA purification.

Successful ChIP-seq experiments for a variety of plants

Arabidopsis

Figure 1. ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with our MicroPlex Library Preparation™ kit from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.

Poplar

Figure 3. ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with the MicroPlex Library Preparation™ kit from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.

Tomato

Figure 2. ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with our MicroPlex Library Preparation™ kit from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.

Maize

Figure 4. ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Libraries were prepared with our MicroPlex Library Preparation™ kit from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.

ChIP-seq
Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomics researchers, namely to investigate protein-DNA interaction on ... Read more

ChIP-qPCR
Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory region... Read more

How to properly cite this product in your work

Characterization of the Polycomb-Group Mark H3K27me3 in Unicellular AlgaeMikulski P. et al.Polycomb Group (PcG) proteins mediate chromatin repression in plants and animals by catalyzing H3K27 methylation and H2AK118/119 mono-ubiquitination through the activity of the Polycomb repressive complex 2 (PRC2) and PRC1, respectively. PcG proteins were extensively studied in higher plants, but their function and ...

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