High-performance liquid chromatography (HPLC) is today the
leading technique for chemical analysis and related applications,
with an ability to separate, analyze, and/or purify virtually any
sample. Snyder and Kirkland's Introduction to Modern Liquid
Chromatography has long represented the premier reference to
HPLC. This Third Edition, with John Dolan as added coauthor,
addresses important improvements in columns and equipment, as well
as major advances in our understanding of HPLC separation, our
ability to solve problems that were troublesome in the past, and
the application of HPLC for new kinds of samples.

This carefully considered Third Edition maintains the
strengths of the previous edition while significantly modifying its
organization in light of recent research and experience. The text
begins by introducing the reader to HPLC, its use in relation to
other modern separation techniques, and its history, then leads
into such specific topics as:

The basis of HPLC separation and the general effects of
different experimental conditions

Computer simulation, qualitative and quantitative analysis, and
method validation and quality control

The separation of large molecules, including both biological
and synthetic polymers

Chiral separations, preparative separations, and sample
preparation

Systematic development of HPLC separations—new to this
edition

Troubleshooting tricks, techniques, and case studies for both
equipment and chromatograms

Designed to fulfill the needs of the full range of HPLC users, from
novices to experts, Introduction to Modern Liquid
Chromatography, Third Edition offers the most up-to-date,
comprehensive, and accessible survey of HPLC methods and
applications available.

Lloyd R. Snyder, PhD, is a Principal at LC Resources in
Walnut Creek, California. He is the author or coauthor of several
books including An Introduction to Separation Science, Introduction
to Modern Liquid Chromatography, Second Edition, the bestselling
Practical HPLC Method Development, Second Edition, and the
comprehensive High-Performance Gradient Elution, all published by
Wiley.

Joseph J. Kirkland, PhD, is Vice President of Research
and Development for Advanced Materials Technology, Inc., and
coauthor of Introduction to Modern Liquid Chromatography, Second
Edition, Practical HPLC Method Development, Second Edition, and
Modern Size-Exclusion Liquid Chromatography, Second Edition, all
published by Wiley.

John W. Dolan, PhD, is a Principal at LC Resources. He is
author of the popular "LC Troubleshooting" column in LCGC magazine
and coauthor with Lloyd Snyder of Troubleshooting LC Systems and
High-Performance Gradient Elution.

"In summary, I would strongly recommend this book for anyone
working with LC and LC/MS. It is a finely crafted introduction that
adequately covers nearly every aspect of the science. The emphasis
on basic principles and practical aspects ensures that it will be a
useful reference for many years to come." (J Am Soc Mass Spectrom,
2011)

"This third edition is highly cross-referenced, so as to allow
the reader to follow up on topics of special interest, or to
clarify questions that may arise during reading. The third edition
of Introduction to Modern Liquid Chromatography will continue to be
the HPLC reference book for thousands of readers, either
experienced workers who may wish to explore topics of his/her
choice, or find an answer to specific problems, or beginners who
would like to understand and know the possibilities offered by
the
technique." (Chemistry Journals, 11 April 2011)

"This classic text on liquid chromatography has been thoroughly
updated through the addition of information on modern
instrumentation, columns, and troubleshooting. It is a valuable
resource for practicing chromatographers at all levels.." (Anal
Bioanal Chem, 2011)

"In summary, I would strongly recommend this book for anyone
working with LC and LC/MS. It is a finely crafted introduction that
adequately covers nearly every aspect of the science. The emphasis
on basic principles and practical aspects ensures that it will be a
useful reference for any years to come." (American Society for Mass
Spectrometry, 21 Januay 2011)

"The text is illustrated with many figures and tables
originating from authors' own work or taken from the literature . .
. both groups of readers will find in this book plenty of
information and inspiration." (Journal of Separation Science, 1
June 2010)

"Following their highly successful second edition (1979), which
appeared more than 30 years ago, Snyder (LC Resources) and Kirkland
(Advanced Materials Technology) have teamed with Dolan (LC
Resources) and additional collaborators to provide an excellent
update of their earlier work." (CHOICE, July 2010)

"It is difficult in these times to ever call a scientific book a
"bargain", but this truly is. The wealth of information contained
in these almost 1000 pages is invaluable. The book is
comprehensive, eminently readable and approachable, and highly
useful for both the skilled chromatographer and those new to the
technique. Everyone using a liquid chromatographic instrument would
benefit from owning a copy." (JACS, 2010)

"It's current, clearly-indexed, well-referenced, and
comprehensive. The inclusion of John Dolan as author has
significantly expanded the sections on troubleshooting, and
additional expert contributors have enhanced the coverage of the
specialist chapters." - Bruce Hamilton posted on the Chromatography
Forum

Connect with Wiley Publicity

Hoboken, NJ —November 20, 2009—Wiley-Blackwell, the scientific, technical, medical and scholarly publishing business of John Wiley & Sons, Inc. (NYSE: JWa), (NYSE: JWb), have announced the publication of Introduction to Modern Liquid Chromatography, 3rd Edition, by Lloyd R. Snyder, Joseph J. Kirkland, and John W. Dolan.

The bookprovides an in-depth explanation of how high-performance liquid chromatography (HPLC), the most commonly used analytical technique in biochemistry, is performed. The text covers the necessary materials involved in the process, as well as its possible applications. The book’s easy-to-read format is designed to be accessible to all HPLC users, from novices to experts.

The popularity of HPLC stems from its ability to separate a large variety of analytes, including organics, inorganics, and biomaterials, with low to high molecular weights and with different degrees of polarity, hydrophobicity, acidity and ionization. The development of highly sophisticated HPLC procedures has provided a sensitivity that allows investigators to accomplish important research in a wide variety of areas. The vast majority of biomedical and environmental analyses are currently performed by HPLC and it is the premier analytical technique in the chemistry field.

Widely referred to as "the Bible of HPLC", the previous edition has become a ubiquitous reference guide. The new edition strives to be more accessible to students, making it not only a reference volume, but also a learning tool. The practical HPLC knowledge included is intended to integrate questions of “what to do” and “why” into a single book, so as to satisfy readers at multiple levels from beginner to expert.

The new edition features the latest HPLC applications and technologies, including chiral compound separations and bioseparations, as well as an update of HPLC computer software and technology. This text presents a comprehensive survey of HPLC methods and applications written by practicing scientists for researchers and students of analytical chemistry and separation science and professionals at all degree levels using liquid chromatography.

Digital version available through Wiley Online Library

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