Materials

Protocol

Place 1 ml of aqueous sample in a 8-ml reaction vial. Add 2.5 ml of methanol. Add 1.25 ml of CHCl3. Vortex. Add 1.25 ml of CHCl3. (note: CHCl3 must be added in two steps)Add 1.25 ml of water. Vortex. The bottom organic phase contains the lipids. Transfer this phase to another tube. Evaporate under N2.