Julian Dunkerton, the co-founder of Superdry, said that he was making the donation to the “People’s Vote” campaign because he saw a “genuine chance to turn this around”.

However Downing Street highlighted a poll in The Sun on Sunday which found that 15 per cent of Remainers have changed their minds, compared to 11 per cent on leavers.

Robbie Gibb, the Prime Minister’s Director of Communications, said: “1.9million Leave voters say they would now vote to Remain. But 2.4million Remain voters would now vote to Leave. The country hasn’t changed its mind.”

Theresa May has repeatedly ruled out a second referendum on Brexit, but there is growing support among Remain backing Tory and Labour MPs. Mr Dunkerton, who left Superdry earlier this year, told the Observer: "If Brexit had happened 20 years earlier, Superdry would never have become the global success that it did. We would have struggled to cope with negotiating customs and tariffs. Perhaps even more importantly, Europe was our staging post because inside the single market we had no fear of opening a store in France, Germany, Belgium or anywhere else."

Andrew Bridgen, a eurosceptic Tory MP, said: "People's Vote talk about democracy. What their declared intent is is to overturn the democratic decision of the 2016 referendum to leave the European Union," Mr Bridgen told BBC News.

"The way the EU have dealt with the UK Government in these negotiations, a large number of former Remain voters have come to the conclusion that we couldn't possibly stay in this relationship any longer.

"It would be rather like returning to an abusive relationship if we stayed in. We would be an absolute doormat."

It comes as the Government is facing a backlash from Eurosceptic Tory MPs over Mrs May's plans for Brexit.

Mr Gibb added: " Nobody else in British politics has a detailed plan for our future relationship with the EU that delivers on the instruction of the people, commands sufficient support in Parliament and is negotiable with the EU.