Hemangioma-derived mesenchymal stem cells (Hem-MSCs) expressed PPAR-Î³, the key transcription factor in adipogenesis. We supposed that rosiglitazone, the agonist of PPAR-Î³, may promote the adipogenesis of Hem- MSCs. In this study, MSCs were isolated from proliferating hemangioma. Four groups were set up, which were Group A (DMEM-LG/10% FBS), Group B (1 Î¼M rosiglitazone + DMEM-LG/10% FBS), Group C (adipogenic media), and Group D (1 Î¼M rosiglitazone + adipogenic media). Cells were cultured in the medium above. On the day 7 and 14, Oil Red "O" staining and Western blot were performed to detect the cytoplasmic lipid and perilipin A in the cells. The results showed that cytoplasmic lipid appeared in Group C and D, and no cytoplasmic lipid in Group A and B on the day 7 and 14. Analysis of Oil Red "O" staining showed the area of staining in Group D was significantly larger than that in Group C. Analysis of western blot showed no expression of perilipin A in Group A and B, and upregulated expression in Groups C and D, with the greater upregulation in Group D. In conclusion, our study demonstrated that rosiglitazone promoted the adipogenesis of Hem-MSCs initiated by adipogenic media via the activation of PPAR-Î³ pathway. The results may put forward the possibility of treating hemangioma via PPAR-Î³ pathway.

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The aim of this study was to isolate human mesenchymal stem cells (MSCs) from the gingiva (GMSCs) and confirm their multiple differentiation potentials, including the odontogenic lineage. GMSCs, periodontal ligament stem cells (PDLSCs) and dermal stem cells (DSCs) cultures were analyzed for cell...

The past decade has witnessed a sudden surge in the obesity prevalence worldwide. Obesity has been linked to several chronic metabolic disorders including diabetes, hyperlipidemia and atherosclerosis. Due to this there is an immense interest in understanding the intricate aspects of...

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