Azithromycin impurity J is primarily used as a reference standard. Reference standards are well characterized compounds that can be used to help identify and/or quantify impurities in pharmaceutical compounds and antimicrobials.

Mechanism of Action

Azithromycin inhibits bacterial growth by binding to the 70S ribosome (specifically the 50S subunit) preventing peptide bond formation and translocation during protein synthesis. Resistance is attributed to mutations in 50S rRNA preventing binding of Azithromycin and allowing the cell to synthesize error-free proteins.