N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Reconstitution

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.

Preservative Stabilisers

0.05%

Sodium Azide

Purity

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Reagents In The Kit

Preparing The Antibody

Test Principle

Buffer Solution

Phosphate buffered saline

Storage

Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

18 months from date of despatch.

Acknowledgements

Regulatory

For research purposes only

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name

Yes

No

Min Dilution

Max Dilution

ELISA

Immunohistology - Paraffin

Western Blotting

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in his/her own system using appropriate negative/positive controls.

Technical Advice

Recommended Protocol

ELISA

Immunohistology

This product does not require protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase prior to staining. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.