Thisstable mutant of rabbit SERPINE1 contains a singleconservative mutation (Isoleucine 91 to Leucine) that yields PAI-1 with anenhanced half-life as compared to the wild type.

Description:

SERPINE1 is aprotein that in humans is encoded by the SERPINE1 gene. SERPINE1 is a serineprotease inhibitor (serpin) thatfunctions as the principal inhibitor of tissue plasminogen activator (tPA)and urokinase (uPA), the activators of plasminogen and hence fibrinolysis(the physiological breakdown of blood clots). It is a serine proteaseinhibitor (serpin) protein (SERPINE1).

Source:

E. coli

Species:

Rabbit

Concentration:

2.5mg/ml

Form:

Liquidin 0.05 M Sodium Acetate + 0.1M NaCl + 1mM EDTA, pH 5.0.

Purity:

>95% by SDS-PAGE.

Molecular Weight:

43 kDa

Endotoxin Level:

<0.1 ng/μg of protein.

Specific Activity:

80%active by titration with tPA.

Storage:

Storeat -80°C. Stable for 3years from delivery. For long term use, divide into working aliquots andfreeze at -80°C. Avoid repeatedfreeze-thaw cycles.