Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

92 kDa (note)

Positive Controls

K562 , THP-1

Form Supplied

Liquid

Concentration

1
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Purified by antigen-affinity chromatography.

Specifications: RBM5 antibody

Full Name

RNA binding motif protein 5

Product Description

Rabbit Polyclonal antibody to RBM5 (RNA binding motif protein 5)

Background

Component of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis.

Immunoprecipitation of RBM5 protein from 293T whole cell extracts using 5 μg of RBM5 antibody (GTX129783).Western blot analysis was performed using RBM5 antibody (GTX129783).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunoprecipitation of RBM5 protein from 293T whole cell extracts using 5 μg of RBM5 antibody (GTX129783).Western blot analysis was performed using RBM5 antibody (GTX129783).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.