Peak F (fractions 46-50) contained at least 2 components in which the later eluting portion (fraction 49) reacted with all 3 BNP antisera, indicating it is a BNP form with intact C- and N-terminal ends.

Microscope slides with fixed sections were treated for 2 hours at room temperature with a non immune serum (goat; Sigma), diluted 1:100 in Phosphate Buffered Saline (PBS), and then incubated overnight at 4[degrees]C with four of two primary region-specific antisera.

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