Minimum of six orders of magnitude. Detection of a synthetic template standard curve from 20 to 20 million copies.

R2

>0.99

These DNA primer pairs were designed by prioritizing the gene regions most commonly found in transcript variants. Strict design criteria were used to ensure optimal real-time PCR results for each target:

Target regions without SNPs

PCR primer pairs annealing across intron/exon junctions when possible

No secondary structure in primer annealing sites

Maximum number of transcript isoforms detected

PCR primers compatible with standard assay conditions

Every PCR primer pair was experimentally validated using Bio-Rad’s iScript™ advanced cDNA synthesis kit and SsoAdvanced™ SYBR® Green supermix. PrimePCR assay design and validation are fully described in the following publication.

Thomson Reuters provided interactive pathway maps for 260 canonical pathways. Each pathway belongs to one or more general biological categories such as cancer. The pathway maps illustrate protein interactions and regulation to provide a comprehensive picture of signaling and disease processes. The selected pathways were used to design panels of real-time PCR primers tailored for the top-ranked genes for differential gene expression analysis. Each gene target within a pathway was assigned a score based on the frequency of differential expression and its research significance. The resulting scores were used to select the assays included in the corresponding real-time PCR pathway panel.

Control assays and synthetic DNA templates were designed to facilitate the assessment of the key experimental factors impacting your real-time PCR results.

DNA Contamination Control AssayUse the PrimePCR DNA contamination control assay to determine if genomic DNA (gDNA) is present in a sample at a level that may affect PCR results. This assay may also be used to compare relative levels of gDNA contamination present in different samples to determine if PCR results may be affected.

Positive PCR Control AssayUse the PrimePCR positive control assay to qualitatively assess the performance of a PCR reaction associated with a single sample. This assay may also be used to compare the relative performance of PCR reactions associated with different samples.

RNA Quality AssayUse the PrimePCR RNA quality assay to determine if RNA integrity may adversely affect PCR results for a single sample. This assay may also be used to compare relative RNA integrity among different samples to determine how PCR results might be affected.

Reverse Transcription Control AssayUse the PrimePCR reverse transcription control assay to qualitatively assess the performance of the reverse transcription reaction associated with a single sample. This assay may also be used to compare the relative performance of the reverse transcription reactions associated with different samples.

Reference Gene AssaysReference genes are used in relative gene expression analysis to normalize for variation in the amount of input messenger RNA (mRNA) among samples. To ensure accurate quantitation, it is important to include one or more reference genes exhibiting constant expression levels under the experimental conditions. To streamline reference gene selection, we offer PCR primers for a set of commonly used reference genes that can be used individually, easily screened using our preplated 96-well and 384-well reference panels or added to custom-designed plates.

Real-time PCR primer assays consist of unlabeled PCR primer pairs for use with dye-based chemistry such as SYBR® Green or EvaGreen®. Probe assays for real-time PCR and Droplet Digital™ PCR include PCR primers and a dual-labeled fluorescent probe with your choice of fluorophore. You can also design custom PCR primer or probe assays.

Find an Assay or Template

PCR Technology (select one)

Detection Chemistry (select one or more)

Application (select one)

Organism (select one or more)

Find an Assay or Template

Select an individual PCR primer or probe assay for PreAmp, qPCR, or ddPCR.

Find a Pathway Plate

Find a predesigned PCR plate for your biological pathway or disease of interest.

PCR Primer and Probe Assays for Real-Time PCR

Our real-time PCR primers were designed in collaboration with leading experts in real-time PCR research. Every PCR primer pair has been experimentally validated to ensure optimal assay performance. For more information on the validation of the DNA primer pairs, see Bulletin 6262, PrimePCR Assay Validation.

Our fluorescently labeled oligonucleotide probes were designed to enable multiplexing. These 5' nuclease probes were designed for maximum specificity and transcript coverage. qPCR probe assays are available with a FAM, HEX, TEX615, Cy5, or Cy5.5 fluorophore.

Probe Assays for Droplet Digital PCR

Droplet Digital PCR (ddPCR™) technology provides an absolute measure of target DNA molecules with unrivaled accuracy, precision, and sensitivity, accelerating discovery and enabling new research strategies for inherited disorders, cancer, and infectious disease.Probe assays for the QX200™ Droplet Digital PCR system enable the fine quantitative discrimination required to resolve small fold changes without a standard curve. ddPCR probe assays are available with a FAM or HEX fluorophore.

For more than 15 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows high school and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices.

How do I apply for the education discount?

If you are an educator at the high school or college level, visit our Education Discount Policy page to establish an education account number.