Manufactured the way you want

Each SNP-In library is built according to your requested parameters, including customizable homology arm lengths and silent mutations to prevent retargeting. Our team can build your library using stock or custom backbones for unique library synthesis.

Parameter

Customizable?

Example

A Case Study

How pharmaceutical researchers used a SNP-In library for target ID

Understanding our client

An industry drug discovery team planned to introduce clinically-relevant variants into tumor suppressor genes. They were looking for a library service that could design both their guide RNAs and corresponding donors.

Made-to-order library specifications

The research team provided Desktop Genetics staff with a comprehensive list of variants and unique parameters for guide and donor design. Our client also requested specific homology arm lengths and locations of silent mutations to prevent retargeting. We then directed DESKGEN AI to design guide RNAs and donor template DNA according their request.

DESKGEN SNP-In Library

Parameter

Approach

Screen type

Arrayed

Reference genome

Human (GRCh38)

Components

sgRNA & ssODN

Homology arm length

40 bp (each)

Silent mutations

PAM & Seed

Vector system

Two-vector

Full documentation

To support further investigation, we also provided our client with a final design report and manifest of all guides and their specifications, including predicted off-target sites and mismatch data.