Buffer storage question - (Oct/08/2008 )

Hi,

I am hoping to try out a new protocol for western blots and for one of the steps, the protocol says to dilute my protein samples in a buffer before loading onto polyacrylimide gel. The buffer in question contains -125mM Tris, pH6.8-2% SDS-10% Glycerol-0.2% Bromophenol Blue-5mM DTT

I am wondering if anyone knows if it would be possible to make up a stock solution of this buffer and freeze/refrigerate aliquots? Or would I have to make the buffer up fresh each time I need the buffer?

Thanks

-clarem-

QUOTE (clarem @ Oct 8 2008, 02:46 AM)

Hi,

I am hoping to try out a new protocol for western blots and for one of the steps, the protocol says to dilute my protein samples in a buffer before loading onto polyacrylimide gel. The buffer in question contains -125mM Tris, pH6.8-2% SDS-10% Glycerol-0.2% Bromophenol Blue-5mM DTT

I am wondering if anyone knows if it would be possible to make up a stock solution of this buffer and freeze/refrigerate aliquots? Or would I have to make the buffer up fresh each time I need the buffer?

Thanks

you should freeze as DTT hydrolyzes within hours; all other ingredients are stable w/o freezing

-The Bearer-

QUOTE (The Bearer @ Oct 8 2008, 11:42 AM)

QUOTE (clarem @ Oct 8 2008, 02:46 AM)

Hi,

I am hoping to try out a new protocol for western blots and for one of the steps, the protocol says to dilute my protein samples in a buffer before loading onto polyacrylimide gel. The buffer in question contains -125mM Tris, pH6.8-2% SDS-10% Glycerol-0.2% Bromophenol Blue-5mM DTT

I am wondering if anyone knows if it would be possible to make up a stock solution of this buffer and freeze/refrigerate aliquots? Or would I have to make the buffer up fresh each time I need the buffer?

Thanks

you should freeze as DTT hydrolyzes within hours; all other ingredients are stable w/o freezing

Great, thanks for your help:) Do you think storage at -20degrees would be sufficient or should I store at -80?