Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

IHC-P

ELISA

WB

Application notes

Peptide ELISA: Antibody detection limit dilution 1:16,000.
IHC-P: Use at a concentration of 1 - 2 µg/ml. In paraffin embedded tonsil, stains scattered cells in the interfollicular area compatible with mast cells.
WB: Use at a concentration of 1-3 µg/ml. Detects a band of approximately 26-28 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK5 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of RET in neuronal differentiation.