Canadian Journal of Pure & Applied Sciences

An International Journal

THE HISTOLOGY OF EPIPHYSEAL PLATE OF DEVELOPING THIGH BONE OF FAYOUMI UNDER THE INDUCED EFFECT OF NICOTINE AND GREEN TEA (CAMELLIA SINENSISPage No: 4549-4552

By: Maryam Shan, Shazia Imran and Hina Shan

Keywords: Incubation, femur, nicotine, hypertrophy zone.

Abstract: Cigarette smoking has become one of the greatest developing health hazard in young people and World Health
Organization showed figures regarding million tobacco-related deaths every year. The present study was conducted
during the period of November 2011 - November 2012 to assessing the induced adverse effects of nicotine. Simple
Random Sampling technique was used. Freshly laid fertilized eggs of Fayoumi species were selected at zero hour of
incubation. Hatchery was used for placing eggs at 37.50C. The proper ventilation and relative humidity of 75% was
maintained. Four groups were made each group was comprising of ten numbers of eggs. Control group G1 was given
normal saline, experimental group G2 was given Camellia sinensis extract, group G3 was given 0.0001% nicotine
solution, group G4 was given both 0.0001% nicotine solution and green tea extract. Double exposure of doses was given.
First exposure was at 48 hours of incubation and second at 48 hours of hatching (post natal dose). At the age of one
month chicks were sacrificed, thigh bones were collected dissected and duly labeled tissue cassettes and slides were
made to observe the height of hypertrophy zone. Experimental groups when compared with each other such as, G2 in
comparison with G3 and G4 showed significant results with p values (0.004) and (0.000), respectively. Comparison of
G3 and G4 with each other showed statistically significant result with p value (0.000). Based present study, we conclude
that nicotine, one of the constituent of cigarette smoke causes toxic effects on the developing thigh bone of chick.

The journal complies the terms and conditions stated in the Creative Common Attribution-Non Commercial Public License 4.0. Authors of the articles are
granted the privileges stated in the public license and the terms and conditions can be accessed via Creative Common License Link.