Click-IT® Edu Now Available in Larger Size

Click-iT® EdU offers a simplified method for detecting nascent DNA synthesis and is now available in a 5-gram size. EdU is an alkyne-containing nucleoside analog of thymidine whose detection is based on a click reaction, a copper (I)–catalyzed reaction between an azide and an alkyne. The small size of the detection azide allows the use of mild conditions to access EdU incorporated into DNA. The Click-iT® EdU assay can be completed in less than 90 minutes, without the need for immunoreagents or signal amplification.

New Mitochondrial Antibodies

Invitrogen™ mitochondrial antibodies can be used to study the role of mitochondria in various cellular pathways and diseases. New antibodies are available for the specific detection of mitochondrial proteins including GAPDH, HSDL2, DCXR, and SOD2. These antibodies are validated for multiple applications including western blots, immunocytochemistry, immunohistochemistry, immunoprecipitation, and ELISA.

CellLight® Lysosomes-GFP

CellLight® reagents are fluorescent protein–signal peptide fusions that permit specific labeling of cellular structures, including lysosomes, for live-cell imaging or for analyses following formaldehyde-based fixation. CellLight® reagents are powered by BacMam technology, which uses a modified insect cell baculovirus coupled with a mammalian promoter to efficiently deliver and express genes in mammalian cells.

PrestoBlue™ Cell Viability Reagent

The PrestoBlue™ Cell Viability Reagent is a simple add-and-read cell viability assay. Once a plate containing cells and compounds is prepared, the PrestoBlue™ reagent is added and results can be read in as little as 10 minutes. The PrestoBlue™ reagent allows for reliable, high-quality cell viability data and continuous live-cell monitoring and assessment after measuring viability.

Qubit® 2.0 Fluorometer: The Next Generation of Nucleic Acid and Protein Quantitation

Designed in response to customer feedback, the new Qubit® 2.0 Fluorometer features an improved user interface that makes the instrument more intuitive and easier to use, while providing the same high accuracy and sensitivity as the original Qubit® Fluorometer. The new features include a larger LCD touch screen, step-by-step workflow navigation, and a USB port and automated data logging for efficient data management. See The Qubit® 2.0 Fluorometer article, in this issue.

New Antibodies for Hyperosmotic Stress Research

FAK phosphorylation at tyrosine 861 has been indicated as a requirement for mammalian cells to survive under hyperosmotic stress. EGFR is also activated in downstream hyperosmotic stress signaling cascades. Invitrogen now offers two new ABfinity™ recombinant rabbit monoclonal antibodies to help elucidate FAK [pY861] and EGFR signaling. These antibodies are validated for western blotting and immunofluorescence applications.