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Thursday, February 21, 2013

CytoTell™ Green

Flow cytometry combined with fluorescence staining is a powerful tool
to analyze heterogeneous cell populations. Among all the existing
fluorescent dyes CFSE is the preferred cell proliferation indicator that
is widely used for live cell analysis. However, there are a few severe
problems associated with the use of CFSE for monitoring cell
proliferation. 1).

CFSE is highly toxic to cells. CFSE indiscriminately reacts with all
amino groups, thus changes many critical intracellular protein functions
(such as cell membrane GPCRs); 2). CFSE has slow response and is
inconvenient to use. The CFSE fluorescence intensity of the 2nd
generation cells is decreased more than 10 fold from the 1st generation.
You would have to wait for another generation to start the cell
proliferation analysis. 3). Medium removal is required. You would have
to remove medium for cell analysis with a flow cytometer since CFSE
reacts with medium components. CytoTell™ Green is developed to eliminate
these limitations. It has distinct advantages over CFSE. 1). CytoTell™
Green has very minimal cytotoxicity since it does not react with
proteins. It is well retained in cells since the probe is designed to
minimize the MDR interaction that generally pumps many cell stains out
cells; 2). CytoTell™ Green exhibits much faster response and is more
convenient to use than CFSE. There is no fluorescence intensity gap
between 1st and 2nd generation of cells. As cells divide, CytoTell™
Green is distributed equally between daughter cells that can be measured
as successive halving of the fluorescence intensity of the dye; 3).
There is no need to remove medium since CytoTell™ Green does not react
with medium components; 4). CytoTell™ Green is more sensitive than CFSE.
Up to 9 generations may be visualized; 5). CytoTell™ Green is much more
stable than CFSE. CytoTell™ Green stock solution can be stored at room
temperature for a few days. CytoTell™ Green can also be used for long
term tracking of labeled cells. Analysis using two-parameter plots may
provide better resolution of each generation, especially between
undivided cells and the first generation. Cells labeled with Cell
CytoTell™ Green may be fixed and permeabilized for analysis of
intracellular targets using standard formaldehyde-containing fixatives
and saponin-based permeabilization buffers. CytoTell™ Green has a peak
excitation of 520 nm and can be excited by the blue (488 nm) laser line,
making it compatible with FITC filter set.

Stratech Scientific is a distributor of high quality, competitively
priced, reliable products for research laboratories throughout the UK
and Europe. Please contact us to find out which ranges we can supply in
your country.