Antibiotic Selection Markers & Plasmids

Linear Selection Markers are ideal for cotransfection with any expression vector where stable integration and expression are required. We offer Linear Selection Markers for either hygromycin resistance (Linear Hygromycin Marker) or puromycin resistance (Linear Puromycin Marker). These markers are short, purified linear DNA fragments comprised of the marker gene, an SV40 promoter, and the SV40 polyadenylation signal.

Cotransfection of a Linear Selection Marker achieves a higher number of positive clones than using either a single response vector containing a selection marker, or cotransfecting with a circular selection marker (1). Linear selection markers are cotransfected at a decreased ratio relative to the expression vector (i.e., 20-fold less of the linear marker).

The pTK-Hyg Selection Vector and the pPur Vector may be used to select for stably transformed mammalian cells using hygromycin and puromycin, respectively. When cotransfected with pTRE2 or a pBI vector, pTK-Hyg is especially useful for selecting double-stable Tet-On or Tet-Off Cell Lines. When integrated into the host cell’s genome, pTK-Hyg does not cause unwanted activation of pTRE- and pBI-derived plasmids because the HSV TK promoter lacks an enhancer element.

Features

Both the Linear Selection Markers and the pTK-Hyg Selection Vector and pPur Vectors may be used in cotransfections to establish stable cell lines

Linear Selection Markers require you to screen fewer clones, resulting in more positive clones than when circular plasmid markers are used

Linear Selection Markers are ideal for use with all Clontech's Tet-On and Tet Off Inducible Expression Systems—screen less and find more highly inducible clones

Applications

Use the Linear Hygromycin Marker or the pTK-Hyg Selection Vector in cotransfections to establish stable cell lines using hygromycin

Use the Linear Puromycin Marker or the pPur Selection Vector in cotransfections to establish stable cell lines using puromycin

References

See "Linear Selection Markers" in "Application Notes" under the "Resources" tab.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.