[pSer473/474]Akt1/2 ELISA kit

Fast - fully quantitative results from up to 41 samples in duplicate in just 3 hours

Reliable - validated with up/down regulation experiments and compared to Western blot

The [pSer473/474]Akt1/2 EIA kit is a colorimetric immunometric enzyme immunoassay kit. Absorbance is read at 450 nm. This is an ultra-sensitive assay that lets you measure as little as 5.5 pg/mL Akt1 or Akt2 phosphorylated on Ser473 or Ser474. Obtain fully quantitative results from up to 41 samples in duplicate in just 3 hours. The assay has been validated with up and down regulation experiments and compared to Western blot.

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Product Specification

Alternative Name:

PKB, Protein kinase B

Sensitivity:

5.5 pg/ml (range 17.5 - 560 pg/ml)

Assay Time:

3 hours

Applications:

ELISA, Colorimetric detection

Application Notes:

For the quantitative determination of [pSer473/474]Akt1/2 in cell lysates of human, mouse, and rat origin.

The Akt (PKB) family of protein kinases are serine/threonine kinases, with three mammalian family members identified to date (Akt1, Akt2, Akt3). Akt is a well-characterized member of PI3 kinase-mediated signaling pathways, regulating cell growth, apoptosis, glycogen synthesis, and other cellular responses through its phosphorylation of downstream substrates. Akt activation is triggered by binding of phospholipid and phosphorylation at two key residues: Thr308 by PDK1, and Ser473/474 by PDK2, now identified as mTOR. Deregulation of Akt signaling has been associated with cancer, diabetes, and schizophrenia. Akt1 is the cellular homologue of the murine thymoma retroviral oncogene v-akt, and its role in anti-apoptotic and pro-mitotic pathways have made Akt a molecular target for anti-cancer therapeutic intervention. Akt activation inhibits apoptosis by phosphorylating the Bcl-2 related protein Bad, and increases p53 degradation by phosphorylating mdm2. Mitotic substrates of Akt include GSK-3β, p21CIP1, and p27KIP1, cell cycle inhibitors negatively regulated by Akt phosphorylation. Akt has been shown to mediate angiogenesis through regulation of thrombospondins, which may cooperate with pro-mitotic and anti-apoptotic functions of Akt to promote tumorigenesis.