I'm trying to run my first SDS-PAGE gel but can't seem to get the gel
solution correct. From the Molecular Cloning lab manual, I've been
trying to make 5 ml of a 10% gel but it's not polymerizing completely.
Although addition of either more ammonium persulfate or TEMED seems to
make it gel properly, why isn't the amounts given in the manual correct?
Are there other factors I need to take into consideration? Any help
appreciated. Thanks.
-Ben
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