Flow cytometry/FACS : Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.

Form Supplied

Liquid

Concentration

1.0
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Protein A purified

Purification Note

From tissue culture supernatant

Specifications: CD163 antibody [EDHu-1]

Full Name

CD163 molecule

Background

The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

Synonyms

MM130 Antibody , M130 Antibody , CD163 molecule Antibody

Specificity

The Binding of EDHu-1 occurs via the N-terminal region of CD163 containing the first three scavenger receptor, Cysteine-rich, SRCR
domains the third domain being critical as, cleavage of this domain at the major cleavage site
ASP-265 abrogates binding to the N-terminal fragment.

Host

Mouse

Clonality

Monoclonal

Clone Name

EDHu-1

Isotype

IgG1

Immunogen

Leucocytes harvested from the pleural cavity of patients with idiopathic spontaneous pneumothorax