"Makes high resolution melt optimization of amplicons very easy. All fragments amplified first time."

Review date: 12 Jul 2011 | MeltDoctor™ HRM Master Mix

High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences.

The MeltDoctor™ HRM Master Mix contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets. Unlike some competitive mixes, the MeltDoctor™ HRM Master Mix does not require additional mixing prior to use, and was developed and optimized solely for HRM applications.

Perform Mutation Scanning HRM analysis can be used to scan for mutations in target genes for the identification of variant samples prior to sequencing analysis (Figure 1). As a mutation scanning technique, HRM offers significant advantages over conventional methods such as Denaturing High- Performance Liquid Chromatography (DHPLC) and Denaturing Gradient Gel Electrophoresis (DGGE). Specifically, the advantages of HRM for mutation scanning include:

- Low reagent consumption, with little waste: HRM requires only a 20 µL PCR reaction for analysis of each sample, eliminating the need for HPLC solvents or DGGE gels - Simple, fast workflow: no additional instrumentation is required after PCR amplification. A high-resolution melt step can be simply added to the end of the PCR profile for immediate analysis - Fast optimization: unlike DHPLC, thermal optimization is not required - Low sample consumption: following HRM analysis, the PCR product can be used directly in a Sanger sequencing reaction