Chemrefer

Norgen’s waterborne parasite detection program “rats”

Water is the source of life. Everyone uses water every day. Water plays an important role in every aspect of our lives. When water is contaminated with parasites, it can cause a variety of diseases. Water pollution is a serious problem on a global scale and can cause serious illness, disability and even death. Common diseases caused by water-borne parasites worldwide include Guinea worm, schistosomiasis, amoebiasis, cryptosporidiosis, and Giardiasis. People get infected with these diseases by drinking or touching water contaminated with certain parasites. The most common are diarrhea caused by parasites, such as Cryptosporidium and Giardia.

Release time: 2020-01-27

Calcium Fluorescent Probes Topic 2-Ratio Type Fluorescent Probes

Earlier we introduced that the fluorescent indicator method can be used to combine the experiment of Ca2 + detection with other technologies, such as combined detection with a flow cytometer, a fluorescence spectrophotometer, or a fluorescence microscope. Ultraviolet light types mainly include Quin-2, Indo-1, Fura-2, etc., the number is small, and visible light types are large, including Fluo-3, calcein, Rhod-2, etc.

Release time: 2020-01-19

AmyJet recommends IP-specific secondary antibodies

Immunoprecipitation (IP) is a method to purify and enrich the protein of interest by using antigen-antibody specific reaction. After the antibody binds to the corresponding protein in the cell lysate or expression supernatant, it is incubated with proteinA / G-coupled agarose or Sepharose beads. The beads-protein A / G-antibody-target protein complex is obtained by centrifugation, and the precipitate is washed. After that, resuspend in the electrophoresis loading buffer, boil for 5-10min, dissociate the antigen from the antibody under the action of high temperature and reducing agent, collect the supernatant by centrifugation, and the supernatant includes the antibody, the protein of interest and a small amount of hybrid protein.

Release time: 2020-01-15

Norgen Food and Milk Pathogen Detection Kit to help you “test”

Campylobacter jejuni is a curved, rod-shaped, and microaerobic Gram-negative bacterium. It is one of the most common causes of gastroenteritis, with diarrhea as the main symptom. Campylobacter jejuni infections are usually caused by inadequately prepared foods, including undercooked meat (especially poultry), untreated water, or raw milk that has not been pasteurized. Traditional identification of Campylobacter jejuni involves culture, but the microaerobic properties of the bacteria make the enrichment process laborious and expensive.

Release time: 2020-01-17

Inventory of Calcium Fluorescent Probes—Help You Take a Good Calcium Film

Conventional fluorescent probes such as Fluo-4 are highly polar acidic compounds and cannot enter cells. At this time, if you want to do intracellular staining, you need to use patch micro-electrode (patch pipette), microinjection (microinjection), cell-introducing loading reagents and other means to assist in achieving drug loading, which is very complicated. For this reason, by combining acetoxymethyl (AM) with the negative group of Fluo-4, this single change both increases the ester solubility and eliminates the negative charge, which greatly improves the cell permeability. It only needs to incubate the cells in the drug-containing culture medium to achieve loading, which is very easy. Once Fluo-4 AM enters the cell, the esterified calcium ion fluorescent probe is hydrolyzed by esterase to restore Fluo-4.

Release time: 2020-01-09

How much do you know about DNA damage detection technology?

There are many methods for detecting DNA damage. According to their principles, they can be roughly divided into three categories: detection of DNA damage based on changes in the physical and chemical properties of the damaged DNA, detection of DNA damage based on molecular hybridization, and detection of DNA damage based on products formed after DNA damage. The first two methods are based on direct observation of DNA damage based on fluorescent labeling, and the detection of DNA damage based on products formed after DNA damage is an indirect reflection of the degree of DNA damage through the detection of markers.

Plant pathogens include fungi, bacteria, nematodes and viruses, all organisms that can cause disease symptoms and significantly reduce productivity, quality and even cause plant death. Pathogens can be introduced and transmitted to host plants in a variety of ways. Bacteria and fungal spores can be transmitted by wind, rain, or they can be transferred from soil to plant tissues by rain. When insects feed on infected host plants and then feed on and eat uninfected plants, they can be used as a vector for pathogens to infect plants. Pathogens can also be transmitted through infected seeds, transplanted or contaminated equipment, irrigation water and humans.

Release time: 2020-01-03

trFluor streptavidin-a fluorescent dye for TR-FRET

Time-resolved fluorescence (TRF) takes advantage of the unique properties of lanthanides in rare earth elements. The lanthanides commonly used in TRF are thorium (Sm), thorium (Eu), thallium (Tb), and thallium (Dy). Compared to traditional fluorophores, they have large Stoke shifts and very long emission half-lives (from subtle to milliseconds), which makes them increasingly important in the field of biological fluorescence applications.

Release time: 2020-01-03

My child has a bad cough, and mycoplasma is the cause!

Mycoplasma, also known as mycoplasma, is 0.1-0.3 μm in diameter. It is the smallest and simplest prokaryote found so far. It can easily pass through the filter membrane and be mixed into the culture system. It is highly polymorphic, with spherical, rod-shaped, silk Shape, branch shape, etc. Usually attached to the surface of the cell membrane. Mycoplasma does not have a rigid cell wall, so ordinary antibiotics do not work at all. Common types in the laboratory are: oral mycoplasma, arginine, mycoplasma hyorhinis, mycoplasma fermentative, mycoplasma human, mycoplasma saliva , Mycoplasma pneumoniae and Mycoplasma piriformis.

Saliva is a mixed liquid composed of salivary gland secretion, gingival crevicular fluid and mucosal exudate, which stores a large amount of DNA, RNA and protein diversity of human oral microorganisms and oral tissues and other parts of the body infected with microorganisms and viruses. Complex biological information. Saliva collection is non-invasive, highly reproducible, safe and cheap, so salivaomics has gradually attracted people’s attention.

Release time: 2019-12-27

You should know this about circulating tumor cells!

Circulating Tumor Cell (CTC) is a collective name for various types of tumor cells present in peripheral blood.It is detached from solid tumor lesions (primary metastases) due to spontaneous or diagnostic operations.Most CTCs enter the peripheral blood Occurrence of apoptosis or being swallowed, a few can escape and anchor into metastases, increasing the risk of death in patients with malignant tumors.

Release time: 2019-12-26

New qPCR detection kit

African swine fever virus (ASFV) is a large double-stranded DNA virus. It is relatively stable in the environment and can remain active in the air for several days. It can survive for a long time in blood, feces and tissues. The cormorant can survive for a long time, and can survive in frozen meat for years to decades. The virus is sensitive to sodium dodecyl sulfonate and high temperature, and can be inactivated at a temperature of 60 degrees for 20 minutes.

Release time: 2019-12-25

Lentiviral Packaging Protocol Runs

Lentivirus vector is a gene therapy vector developed based on HIV-1 (Human Immunodeficiency Virus Type I virus). Different from ordinary retroviral vectors, it has the ability to infect both dividing and non-dividing cells. Therefore, in the study of in vitro experiments and in vivo experiments, lentivirus has become one of the commonly used vector forms for expressing foreign genes or foreign shRNAs, and it is getting more and more widely used.

Saliva is produced by the glands of the oral cavity, and has many functions such as cleaning and protecting the oral cavity, antibacterial, and digestion. With the development of salivaomics, scientists have discovered that saliva is a potentially huge repository of biomarkers. Saliva samples are easy to collect, non-invasive, and can avoid virus transmission. It is expected to become a blood substitute in epidemiological studies, and has high research value.

Release time: 2019-12-20

ATPase / GTPase activity detection tools are here

ATPase, also known as adenosine triphosphate, is a type of enzyme that can catalyze the hydrolysis of adenosine triphosphate (ATP) to adenosine diphosphate (ADP) and phosphate ions. In most cases, energy can be used to drive another chemical reaction that requires energy. This process is widely used by all known life forms.

Release time: 2019-12-19

Miraculous secreted luciferase (2), do you know

In recent years, a Gaussia secreted luciferase derived from the marine copepod Gaussia princeps has been found to be the smallest naturally secreted luciferase in the body. Because of its secretion, easy monitoring, excessive sensitivity, short half-life, and real-time monitoring of living cells or organisms, it is studying protein-protein interactions, protein localization, small interfering RNA (siRNA) silencing technology, and tumor model drug therapy. It is widely used in evaluation, monitoring of promoter activity, real-time monitoring of tumorigenesis and development, and high-throughput drug screening.

Congratulations, before you started your research, you saw this most reliable explosion evaluation!

Do you often encounter the following problems when conducting scientific research: 1. My RNA sample size is very small. I only want cytoplasmic RNA or nuclear RNA, which is total RNA. 2. I want to measure the methylation level of m6A on RNA. I don’t want to use complex liquid chromatography mass spectrometry (LC-MS) method. 3. I want to study degenerative neurological diseases. I don’t want to use the traditional silver plating method.

Release time: 2019-12-10

Magical luciferase (a), know it or not

At present, the most representative are firefly luciferase isolated from beetles (Firefly luciferase) and Renilla luciferase isolated from Renilla …

Release time: 2019-12-10

Agrisera Plant Membrane Transport System Antibodies, Helping You Unveil the Veil from Outside to Inside

Membrane transport refers to the collection of mechanisms that regulate the passage of solutes, such as ions and small molecules, through biofilms. Biofilms are lipid bilayers in which proteins are embedded. Regulation across the membrane is due to the permeability of selective membranes, a feature of biological membranes that enables them to separate substances with different chemical properties …

Release time: 2019-12-06

Ultra-sensitive fluorescent probe, active oxygen has nowhere to look!

Reactive oxygen (ROS) refers to a general term for substances that are composed of oxygen in the body or in the natural environment and contain oxygen and are active in nature.

Release time: 2019-12-04

In one step or from scratch, GeneCopoeia clones meet all your imagination

GeneCopoeia offers 140,000 ready-to-use Nextday-ORF ™ clones. No need to wait a few days / weeks for immediate gene or protein expression experiments. Each Nextday-ORF ™ clone is sequence verified and delivered the next day, with multiple viral and non-viral vectors available.

Plant developmental biology is the study of individual plant development and its regulatory mechanism. Higher plant individual development begins with fertilization, through cell enlargement, division, differentiation, and apoptosis; through embryogenesis, organ formation, flowering, and pollination and fertilization, a very complex and orderly process is formed.

Cell transfection refers to a technique for introducing foreign genes such as DNA and RNA into cells. According to the mammalian cell protein expression process, cell transfection is required after cell culture is completed.