1b.Approach (from AD-416)
The 'HoneySweet' plum genome will be sequenced. Assembly will be performed using the finished peach genome as a template. Transgene insertion events will be identified via BLAST. Existing Prunus reference markers will be tested on 'HoneySweet' to refine the assembled physical map and determine the chromosome locations of transgene insertion events. Fruit compositional studies will be conducted and compared with control non-genetically engineered plums.

3.Progress Report

Fruit collection plans have been developed to provide material for fruit quality analyses of both 'HoneySweet' and non-transgenic plums grown in Europe.