Dear all,
I wanna isolate receptor protein from culture cell. The theoretical
pI/Mw of that protein is 8.53/78KDa. I've tried several methods but I
couldn't get any postive signals from my western blotting. I think it
maybe the result of low abundance of that protein on these cell. Can
anybody give me some suggestion on the receipe of buffer and better
methods for me to isolate these protein?
Daniel
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