inducation with lamda CE6 - (Jun/28/2006 )

Its necessary that when transforamtion in non-DE3 competent cell you have to induce the cell with lamda CE6 cell. I want to ask for inducation with lamda CE6 you have to grow the culture in NZY broth or you canjust grow in simple LB media.sugestions are appreciated.regardssamad

-samita-

Lambda infects cells which are expressing the maltose binding protein, and has Mg++ as a cofactor. You should culture cells to be infected in a magnesium containing medium with maltose as the carbon source.