Results :
High fat diet led to a significant increase of body weight and hyperlipidemia in mice. Oil Red O staining showed accumulation of lipid in meibomian gland acinus in HFD mice. HE staining showed an increase in periglandular cell infiltrates in HFD mice. Increased infiltrations of CD45+ and CD11b+ cells were observed in meibomian gland in HFD mice than those observed in SD mice. The Raman test results showed lipid composition of tear in HFD mice differs from that of SD mice. Meibomian glands of HFD mice exhibited decreased PPAR-γ expression, increased production of inflammatory cytokines and significant increase in phosphorylation of MAPKs and NF-κB p65 compared with SD groups. 4 weeks of HFD mice shift to SD for 4 weeks showed reversed phenotype, decreased the production of inflammatory cytokines, suppressed the activation of MAPK and NF-κB p65 pathway, with increased expression of PPAR-γ in meibomian gland compared with the HFD mice. In HFD-rosi mice, rosiglitazone treatment stimulated the expression of PPAR-γ,decreased the expression of inflammatory cytokines and inhibited the phosphorylation of MAPKs and NF-κB p65 in meibomian gland.