Characteristics of Relations Between Probiotic and Pathogenic Strains Cause Subclinical Mastitis in Cows

*Corresponding Author :Korablyeva Tatyana RafailovnaDoctor of Veterinary Sciences, Head of the department of microbiology, epidemiology and veterinary-sanitary examination of Academy of Life and Environmental SciencesCrimean Federation University by V.I. Vernadskiy, Russian Federation E-mail:[email protected]

Abstract

The aim of our study was the investigation of biological properties of pathogens which cause various forms of mastitis and the establishment of antibiotic resistance and antagonistic activity of B. subtilis against allocated strains of patogenic microorganisms, and develop a treatment regimen subclinical mastitis in cow’s probiotic Vetom-3. Monocultures were isolated from 11 samples of udder secretion. Studies have shown that the subclinical form of mastitis caused by Staphylococcus spp. Staphylococcus aureus were isolated in 16.7%, Staphylococcus saprophyticus – 8.6 %). Cultures of microorganisms isolated from 22 samples of mammary ecretion were represented by the following associations: Staphylococcus aureus + E. coli - 11.5%, Staphylococcus aureus + Enterococcus faecalis - 3.4%, Staphylococcus aureus + E. coli + Enterococcus faecalis - 2.1%, Staphylococcus aureus + E. coli + Enterococcus faecalis + Staphylococcus saproph. - 5.4%, E. coli + Enterococcus faecalis - 10.2%. It was found that the isolated microflora was highly sensitive to cefotaxime, neomycin, enrofloxacin; to a lesser extent - gentamicin, tetracycline, erythromycin, rifampicin, streptomycin, penicillin, ampicillin; isolates were resistant to ?olymyxin. The B. subtilis strain has a broad spectrum of antagonistic activity to pathogenic bacteria causing mastitis in cows. In the group of animals with a subclinical mastitis, which were treated by the probiotic preparation Vetom-3, the recovery mean time composed 4 days and a positive therapeutic effect was obtained in 85.0% of the cows. Bacteriological examination of udder secretion found out that patogenic microorganisms were isolated from 25% of the samples only.