RNase and RNase A? - (Jan/24/2006 )

What is the difference between RNase and RNase A? Except that they are prepared differently?? I am doing this chromosomal DNA preparation and the Promega protocol says I should use RNase solution provided but then at the end of the protocol they talk about preparing RNase A? I am confused

-smoochiepie79-

There are different types of RNase.

The Promega kit probably just didn't bother to label the RNase as RNase A.

Is this a gen DNA prep?

-Matt

-MisticMatt-

QUOTE (MisticMatt @ Jan 25 2006, 03:02 AM)

There are different types of RNase.

The Promega kit probably just didn't bother to label the RNase as RNase A.

Is this a gen DNA prep?

-Matt

Yes, it is a gen DNA prep. It's the worst kit ever, it doesn't even give you the composition of the buffers, we are never going to buy it again! It worked good so far since I got the thread-like DNA but today we are going to check it on an agarose gel, hope it works. My mentor was also surprised that they suggest we keep this RNase Solution they call it on room temperature. Strange. I was just worried if I should'e used that RNase A instead of this solution, but I have nothing in the kit with RNase except that RNase Solution and the protocol states I should add RNase Solution as it is provided. But it's a very, very confusing kit and I cannot understand that they can sell things like this! I was looking at the bottles of QIAminiprep for the palsmid prep and they say even what are the buffers for each every step actually doing in the kit and how you should add ethanol and RNase. It's just weird and frustrating!

-smoochiepie79-

RNAse A is digesting all RNA and used in general to get rid of RNA while RNAse H cuts specifically RNA-DNA-Hybrids and is used in reverse transcription reactions to prevent primer-mimicking.

-wincel-

Hi, are you talking about Wizard Genomic DNA Purification Kit from Promega? We are using this kit in our lab, and it has always worked fine for us. As far as RNase goes, they provide RNase with their kit, that is stored at RT, and it works fine too. I believe that in this case they use RNase and RNase A interchangeably. When we run out of RNase that they provide with the kit, we also use 5ul of our stock of RNase A (10mg/ml, store at -20) per prep. We isolate bacterial genomic DNA, and this kit works great for it. I am not sure what kind of DNA you are isolating? Hope that this helps.