Bottom Line:
In conclusion, lineages A of HPV16/18 were predominant in Spain.Lineage D of HPV16 was associated with increased risk for CIN3+, glandular high-grade lesions, and invasive lesions compared with lineage A.Lineage B of HPV18 may be associated with increased risk for CIN3+ compared with lineage A, but the association was not significant.

pone-0104678-g005: Phylogenetic tree of the HPV18 isolates based on LCR/E6 sequences.Phylogenetic analysis confirmed the presence of two lineages [4]: A and B. A maximum likelihood (ML) tree was inferred from an alignment of nine reference sequences and seven study sequences of HPV18 LCR-E6 using RAxML HPC v8 [22]. Highly related sequences (<0.4 differences) from the study were not included in this figure. Reference sequences were denominated as lineage/strain.

Mentions:
Two lineages previously described of HPV18 were found in the studied population (Fig. 4 and 5). HPV18 clustered in lineage A (32/36, 88.9%) and lineage B (4/36, 11.1%) (table 3). Lineage B and lineage A strains were respectively detected in 1/23 control patients and 2/5 CIN3+ cases (OR = 9.2, 95%CI: 0.4–565.4, p = 0.12, control group as reference) (table 3).

pone-0104678-g005: Phylogenetic tree of the HPV18 isolates based on LCR/E6 sequences.Phylogenetic analysis confirmed the presence of two lineages [4]: A and B. A maximum likelihood (ML) tree was inferred from an alignment of nine reference sequences and seven study sequences of HPV18 LCR-E6 using RAxML HPC v8 [22]. Highly related sequences (<0.4 differences) from the study were not included in this figure. Reference sequences were denominated as lineage/strain.

Mentions:
Two lineages previously described of HPV18 were found in the studied population (Fig. 4 and 5). HPV18 clustered in lineage A (32/36, 88.9%) and lineage B (4/36, 11.1%) (table 3). Lineage B and lineage A strains were respectively detected in 1/23 control patients and 2/5 CIN3+ cases (OR = 9.2, 95%CI: 0.4–565.4, p = 0.12, control group as reference) (table 3).

Bottom Line:
In conclusion, lineages A of HPV16/18 were predominant in Spain.Lineage D of HPV16 was associated with increased risk for CIN3+, glandular high-grade lesions, and invasive lesions compared with lineage A.Lineage B of HPV18 may be associated with increased risk for CIN3+ compared with lineage A, but the association was not significant.