About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Alternative to gene target
epidermal growth factor receptor, ERBB and ERBB1 and HER1 and mENA and PIG61, EGFR and IDBG-16933 and ENSG00000146648 and 1956, transferase activity, nuclei, Egfr and IDBG-151194 and ENSMUSG00000020122 and 13649, EGFR and IDBG-644035 and ENSBTAG00000011628 and 407217

Antibody's recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.

Tested applications
To be tested

Antibody's datasheet
Contact our support service to receive datasheet or other technical documentation.

Total weight (kg)
1.4

Stock availabilit
In Stock

Scientific context
EGFR is a receptor-tyrosine kinase that is activated upon binding epidermal growth factor (EGF) and transforming growth factor-alpha (TGFalpha). Binding of EGF ellicits a pro-growth and anti-apoptotic response through the Ras pathway. Missense mutations and deletions are common in a variety of cancers.

Antibody's target
Human EGFR (pTyr1172)

Certificate of analysis
A 1:250 dilution of SPC-960 was sufficient for detection of EGFR (pTyr1172) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Tissue specificity
See included datasheet or contact our support service.