Target

FunctionPlays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.

Sequence similaritiesBelongs to the DNA polymerase type-B family.

Post-translationalmodificationsA 165 kDa form is probably produced by proteolytic cleavage at Lys-124.

Thank you for your patience. For ab57296, the sequence homology of the immunogen with mouse is 83% - which is good.The lab let me know that for ab65009, the amino acid range of the immunogen is within residues 100-300 of human DNA Polymerase ...

I am checking with the lab about the homology of the immunogen sequence with mouse. The overall homology between human and mouse is 88%, but some regions align better than others.I will let you know when I hear back.What applications are...