Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

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Mass (in vial)

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Desired Reconstitution Concentration

Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Background: TMED1

TMED1 (Transmembrane Emp24
domain-containing protein 1) is a member of the TMED family of proteins (gene
name TMED1). The TMED family of proteins are localized to membranes of the
early secretory pathway, including the endoplasmic reticulum and Golgi, and function
in vesicular protein trafficking (1, 2). TMED1 is a 59 kDa monomer and has been
reported to exist as homodimer (3). TMED1 is composed of a 23 amino acid (aa) signal
sequence, a 171 aa extra cellular domain, a 21 aa transmembrane domain, and a
12 aa cytoplasmic domain. The extracellular domain contains an 83 aa GOLD
(Golgi Dynamics) domain, and COPI and COPII binding motifs are found in the
cytoplasmic domain (1-3, 5). Human TMED1 shares 97% sequence identity with
mouse, bovine, and rat homologs within the 171 aa extracellular domain. The
beta -strand-rich GOLD domain has been specifically identified to be involved in
intracellular protein trafficking (1, 4, 5). TMED1 is important in regulating
innate immune signaling through its interaction with ST2L. Specifically, the
GOLD domain in TMED1 interacts with the TIR domain of ST2L, a receptor for
IL‑33 (1). This interaction promotes ST2L association with IL-33, allowing
downstream signaling cascade activating MAP kinases, p38, and JNK (1, 6). Studies
have shown knockdown of TMED-1 in HUVECs impairs the IL-33 induced response
resulting in reduction of IL-6 and IL-8 productions (1).