Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant protein produced in yeast, corresponding to the 69 amino acids of the mature swine CCL4/MIP1 protein.

Specificity

This antibody is specific for swine CCL4 protein. A BLAST analysis was used to suggest cross-reactivity with CCL4 from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against horse and panda CCL4 based on 98% homology, with bovine, dog, and rabbit based on 97% homology, macaque and feline based on 95%, with human and chimpanzee based on 94%, and with rat and opossum CCL4 based on 91% homology. Cross-reactivity with CCL4 from other sources has not been determined. The swine CCL4 sequence is also 86% homologous to swine CCL3L1.

Usage

This protein-A purified antibody is suitable for ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 7.8 kD in size corresponding to swine CCL4 protein by western blotting in the appropriate cell lysate or extract.

Presentation

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitution

deionized water

Storage

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.