Mouse IL-28B produced in E. coli is a single, non-glycosylated polypeptide chain containing 174 amino acids and having a molecular mass of 19.6kDa. The IL28B is purified by using an optimized multi-step FPLC method for maximum separation from contaminants.

Mouse IL 28B Bioactive Protein Product Attributes

Protein Construction: A cDNA sequence encoding the sequence of IL 28B was constructred and used to recombinantly synthesize the protein.

Recommended Applications: Bioactive

Application Notes

Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized IL-28B in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Bioactivity Data: The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis is less than 30 ng/ml, corresponding to a specific activity of > 3.3 Ã 104 IU/mg.

Storage Conditions: Lyophilized IL-28B although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL28B should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Limitations and Performance Guarantee

enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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