hello thank you for your response Does gene targeting has been successful in human being in much accuracy?can you please explain in short about cis and trans.Actually i am trying to know more about gene targeting so if you dont mind can you explain.Can retro virus can also be used as vector?

My scientific studies have afforded me great gratification; and I am convinced that it will not be long before the whole world acknowledges the results of my work.-Gregor Mendel

It might be possible (in theory, though it would not be allowed) to remove a gene from a gamete or a single-celled zygote. However, in an adult human you would have to remove the gene from every cell, and there is no technology available to alter the DNA in so many cells at once.

oh ok sir thank you actually.If you dont mind can you say the ways gene targeting is done.This may be silly question but i am just doing my 12th grade so please tolerate with me sir.thank you for your time.

My scientific studies have afforded me great gratification; and I am convinced that it will not be long before the whole world acknowledges the results of my work.-Gregor Mendel