Breaking signals of secondary structure put strong limitations on the tertiary structures of proteins. In addition to proline and glycine clusters, which are well-known secondary structure breakers, clusters of amphiphilic residues were found to be a novel type of secondary structure breaker. These secondary structure breakers were found to depend on specific environmental factors. Such conditions included the average hydrophobicity, the helical periodicity, the density of serine and threonine residues, and the presence of tryptophan and tyrosine clusters. Principal component analysis of environmental factors was conducted in order to identify candidate breakers in the secondary structure breaking regions. Predicted breakers were located in breaking regions with an accuracy of 72%. Taking the loop core into consideration, almost 90% of the predicted breakers were located in the loop segments. When the migration effect of the breaking point was taken into account, the loop segments with the predicted breakers covered two thirds of all loop segments. Herein, the possibility of secondary structure prediction based on secondary structure breakers is discussed. The system of the present method is available at the URL: http://bp.nuap.nagoya-u.ac.jp/sosui/sosuibreaker/sosuibreaker_submit.html.

Mentions:
Three regions around the termini of secondary structures were defined for statistical analysis of the main features in the first step; the breaking region, the secondary structure core, and the loop core (Fig. 1). The breaking region is defined in this work by six residues containing the three terminal residues of the secondary structure or the three terminal residues of a loop. Branching of the same potential breakers to different local structures is thought to be due to the different environmental conditions present in the amino acid sequence. Therefore, all potential breakers were classified into the three regions and the difference in the environments of the potential breakers was investigated.

Mentions:
Three regions around the termini of secondary structures were defined for statistical analysis of the main features in the first step; the breaking region, the secondary structure core, and the loop core (Fig. 1). The breaking region is defined in this work by six residues containing the three terminal residues of the secondary structure or the three terminal residues of a loop. Branching of the same potential breakers to different local structures is thought to be due to the different environmental conditions present in the amino acid sequence. Therefore, all potential breakers were classified into the three regions and the difference in the environments of the potential breakers was investigated.

Breaking signals of secondary structure put strong limitations on the tertiary structures of proteins. In addition to proline and glycine clusters, which are well-known secondary structure breakers, clusters of amphiphilic residues were found to be a novel type of secondary structure breaker. These secondary structure breakers were found to depend on specific environmental factors. Such conditions included the average hydrophobicity, the helical periodicity, the density of serine and threonine residues, and the presence of tryptophan and tyrosine clusters. Principal component analysis of environmental factors was conducted in order to identify candidate breakers in the secondary structure breaking regions. Predicted breakers were located in breaking regions with an accuracy of 72%. Taking the loop core into consideration, almost 90% of the predicted breakers were located in the loop segments. When the migration effect of the breaking point was taken into account, the loop segments with the predicted breakers covered two thirds of all loop segments. Herein, the possibility of secondary structure prediction based on secondary structure breakers is discussed. The system of the present method is available at the URL: http://bp.nuap.nagoya-u.ac.jp/sosui/sosuibreaker/sosuibreaker_submit.html.