I transformed pBEST-PA-UTR1-deGFP-T500 ligation product into JM109. Plates showed many colonies. -linker negative control showed fewer colonies, as expected. Six colonies were picked from the plate for miniculture.

I transformed pBEST-PA-UTRA-deGFP-T500 ligation product into JM109. Plates showed many colonies. -linker negative control showed fewer colonies, as expected. Six colonies were picked from the plate for miniculture.

Hypothesis 2: Gene L is necessary for phage propagation.

Whole plasmid PCR works with the control plasmid and primers included in QuickchangeII kit.