I was considering removing the 59001m emission filter to get a better output range for autofl but is neatly glued in place in the same frame as the bs mirror. The 59001x (excitation filter) can be quite easily removed and then I could add a mercury light for some UV excitation.

Last edited by Pitufo on Fri Feb 09, 2018 11:33 am; edited 4 times in total

Very nice!
Actually I can't see any stereo effect although the single image is pretty 3D itself
Congrats, these multiband filters are usually very expensive

Quote:

These are the specs of the cube. If anyone has suggestions of what else to look at I would be interested.

Not sure about what are you asking
- about the excitation light, this will work very well for UV and V excitation producing both blue and green fluorescence. UV LEDs are now enough powered to avoid the need of mercury lamps, Nichia, LG and LedEngin have adequate models in the 380 - 400nm range.
Long blue (not "royal blue") and many white LEDs would also be good excitation sources for your filter set.

- about subjects, with UV you will find lots of autofluorescence in plants, arthropods and fungi. Pollen of some species, molds and fern sporangia will produce nice emission with blue excitation

If the filter cube is from Chroma it can be dismounted, although it is so well matched that I wouldn't do it.
Time ago I bought a set of Chroma filter cubes for Nikon at bargain price but the filters were glued and I needed to dismount them to adapt the filters to my Zeiss cubes. I asked Chroma -very kind people- and they explained me how to dismount them, just with careful pressure as the glue is like a kind of very soft rubber. I was reluctant to do it -the filters are very delicate- but it worked well, I could forward you the instructions if you want._________________Pau

I realised I hadn't been very clear with my question and was in the process of editing it to include the world "subject".

I have a mercury lamp available so will try that for UV excitation in the short term. UV LED's sound like a much better idea though.

I did look at some plant cells today with my halogen with no success. I have just had a look at the halogen output spectrum and it must be too long wavelength to stimulate autofl in chlorophyll (I couldn't find the exact nm range during a quick search).

Last edited by Pitufo on Fri Feb 09, 2018 1:22 pm; edited 1 time in total

At first look it seems very good and it is in 410nm cut but not so much at the long blue peak were you get up to 10% of the excitation light passing. Because fluorescence emission is much weaker than excitation this likely will produce a blue illuminated background obscuring fluorescence.
If dichroic beamsplitters where perfect emission filters will not be needed (and with lasers sometimes they are not used) but i fear this is not your case.
Substituting it with a longpass 500 or 510nm filter you will have good chlorophyll emission.