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Miltenyi Biotec distribution:

As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is
committed to providing our customers around the world with the highest quality products.
In addition to direct selling in more than 20 countries in North America, Europe and
Asia/Pacific, Miltenyi Biotec also provides support for our customers through an
extensive distributor network covering dozens of additional countries.

As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is
committed to providing our customers around the world with the highest quality products.
In addition to direct selling in more than 20 countries in North America, Europe and
Asia/Pacific, Miltenyi Biotec also provides support for our customers through an
extensive distributor network covering dozens of additional countries.

Application protocol

Viral transduction of human hematopoietic stem cells (HSC)

In this application protocol, we describe the viral transduction of human HSCs isolated from various sources, their concomitant expansion in defined cell culture conditions, and analysis of their function using flow cytometry or colony-forming assays.

MACS Columns and MACS Separators: CD34+ cells can be enriched by using MS, LS, or XS Columns (positive selection). Cells that strongly express the CD34 antigen can also be depleted using MS, LS, or XS Columns. ▲ Note: Positive selection or depletion can also be automated using the autoMACS Pro or the autoMACS Separator.

High purity CD34+ cells isolated from a PBMC sample using the CD34 MicroBead Kit UltraPure. CD34+ cells were isolated from a debris-rich PBMC sample using the CD34 MicroBead Kit UltraPure, two MS Columns, and a MiniMACS™ Separator. Cells were stained with CD34-PE, CD45-FITC and propidium iodide. Cells were analyzed after gating on propidium iodide–, lymphoid cells.

High purity CD34+ cells isolated from a PBMC sample using the CD34 MicroBead Kit UltraPure

CD34+ cells were isolated from a debris-rich PBMC sample using the CD34 MicroBead Kit UltraPure, two MS Columns, an MiniMACS Separator. Cells were stained with CD34-PE, CD45-FITC and propidium iodide solution. Cells were analyzed after gating on propidium iodide–, lymphoid cells.

Reduced debris carry-over during CD34+ cell isolation. A comparison of the ungated forward scatter versus side scatter profile of the same sample after separation with the CD34 MicroBead Kit or the CD34 MicroBead Kit UltraPure demonstrates reduced debris carry-over with the CD34 MicroBead Kit UltraPure.

Reduced debris carry-over during CD34+ cell isolation

A comparison of the ungated forward scatter versus side scatter profile of the same sample after separation with the CD34 MicroBead Kit or the CD34 MicroBead Kit UltraPure demonstrates reduced debris carry-over with the CD34 MicroBead Kit UltraPure.