HIV POC testing by ssDNA coupled with NALF

A isothermal amplification termed Reverse Transcription Loop Dependant Amplification (RT-LDA) was developed with an affordable nucleic acid lateral flow detection (NALF) system, as one component of a potential POC HIV-1 RNA assay for subtypeC. RT-LDA makes use of a primer design that efficiently converts viral RNA into ssDNA amplicons, in 1 hour at 53ºC. Due to the single stranded nature of the product, the amplicon could be detected using NALF.

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The immune system is a striking example of an integrated information system, engaged in coordinated host-protective activities. Organs-on-chip approach (OOC) models allow the direct simultaneous observation of hundreds of different cells, moving, interacting and responding to signals coming from the microenvironment nearby, that give access to a number of parameters describing the system that must be properly measured and elaborated.