About this Product :

MyTaq One_Step RT_PCR Kit PCR kit use certain PCR kits can be used for diagnostics. We supply CE approved PCR kits or research PCR kits. You can request more information about the use of MyTaq One_Step RT_PCR Kit to info@gentaur.com http://mybiofast.com/ver.php?search=pcr&submit=Search

More Details about

"When we compared the performance of our routine supplier’s RTase against Bioline’s MyTaq One-Step RT-PCR, the other supplier’s RTase gave two false negatives in five different grapevine samples tested for Grapevine rupestris stem-pitting-associated Foveavirus. We were convinced to immediately switch."

- The University of Adelaide, Australia

Features

Extremely sensitive blend of RT and novel hot-start MyTaq

Highly optimized for detection of low-copy genes

Overcomes secondary structure in difficult and GC-rich targets

High-quality, full-length cDNA from as little as 3pg of total RNA

Simple to use all-in-one mix

Applications

Gene-expression analysis

Transcription analysis

Gene cloning

Multiplex RT-PCR

Description

MyTaq™ One-Step RT-PCR Kit has been designed for extremely sensitive and highly reproducible first-strand cDNA synthesis and subsequent PCR in a single tube (Fig. 1), from either total RNA or mRNA using gene-specific primers. The kit contains the latest advances in buffer chemistry, including Bioline´s ultra-pure dNTPs, together with reverse transcriptase (RT) and our new generation of very high performance, antibody-mediated hot-start DNA polymerase (MyTaq HS). This ensures that MyTaq One-Step RT-PCR Kit produces fast, highly-specific and ultrasensitive products for downstream applications.

MyTaq One-Step Kit consists of reverse transcriptase, 2x MyTaq HS Mix and a potent RNase Inhibitor, RiboSafe, that are added together to create a simple to use all-in-one mix.

The kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative, semi-quantitative or quantitative analysis of RNA transcription levels, and the one-step format is also perfect for the synthesis of double-stranded cDNA products for subsequent gene-expression analysis.

The cDNA can be synthesised with starting amounts of RNA template from 3pg to 1µg, over a broad temperature range (up to 50°C (Fig. 2) to overcome secondary structure and GC-rich sequences), prior to heating to 95°C to inactivate reverse transcriptase and simultaneously to activate the MyTaq™ HS.