i had ran a PI chip yesterday.And today i found that there was something wrong in the report.Just as shown in the pdf file,a short reads-about 30 bps was mixed into the final library.could anybody give me some suggestion about the origin of the short reads.Thanks!

Thanks for your help!I had send the bam files to the technical support.His anwser was that the short reads were primer dimers.He suggested us to optimize the experiment protocal.So we will improve the experiment pipeline and run one sample on the PGM to test the propose!