Premade Phage Display Peptide Libraries

Besides using the publically available peptide libraries, Creative Biolabs also provide various peptide libraries that were constructed in house:

We carry two good linear peptide libraries: one is 16-mer and the other is 20-mer. They are based on the same true phage system as NEB Ph.D. peptide libraries. In theory, there are about 3-5 copies of peptide on each phage virion.

TriCo-16™ Phage Display Peptide Library

Type: pIII-fusion, True Phage Display

Format: 16-mer peptide library

Capacity: 2.60×1010

Trimer codons technology

Avoid stop and non-sense codon

Trimer codon for cysteine was excluded

TriCo-20™ Phage Display Peptide Library

Type: pIII-fusion, True Phage Display

Format: 20-mer peptide library

Capacity: 1.70×109

Trimer codons technology

Avoid stop and non-sense codon

Trimer codon for cysteine was excluded

As validated by quality control DNA sequencing, more than 97% of randomly picked clones from these two libraries contained dsDNA inserts, and only an insignificant percentage of them harbored longer or shorter diversified peptides. None stop codons were founded as expected. Also, there was no single nucleotide deletion or insertion detected; importantly, non-sense frame-shift mutation was completely absent, demonstrating the beauty of trimer-codon strategy. Moreover, no Cys encoding codon was identified in the peptide library as designed. These Random Peptide Libraries are the best random 16-mer and 20-mer peptide libraries in terms of their quality and diversity.

Creative Biolabs also provide the following specific peptide libraries: