Enhancement of High-Performance Graphene Biosensors for

Backgrounds Goals Elevated alpha-fetoprotein (AFP) levels are tied to issues with the Develop graphene biosensor capable of detecting low levels of alpha- liver cancer and ovarian cancer1. Enhanced detection of AFP would fetoprotein. We will use graphene because it is a 2D material, and allow for quicker medical responses. therefore hyper-sensitive to its environment Current tests such as ELISA are complicated, slow, and require fine Detect AFP concentrations in a matter of minutes tuning for antigen being detected Lower 1/f noise levels via graphene-gold interface redesign FabricationProcess

Graphen Polyelectrolytes PDDA e 5 Tunable graphene thickness cycles Tunable optical, mechanical, and electrical properties Graphene self assembly is possible on various substrates Graph 1 As pH is increased the resistance Large surface area graphene increases. Sensitivity in the reverse in poor Mass production is realistic unlike with traditional methods because of H+ contamination on surface.

AFPDetectionTest Functionalization Conclusions

1. Coat Graphene with Poly-L-lysine AFP Sensing: Our data showed Graph 2: AFP concentrations Helps Anti-AFP bind to Graphene the sensors that were fabricated 1 pg/ml were slowly increased by one 5200 order of magnitude at a time. 2. Functionalize surface with Anti-AFP had at least the same sensitivity as 10 pg/ml

Large spikes in graph are due 3. Fill gaps with Bovine Albumin Serum 100 pg/ml 5000 to pressure changes when Keeps unbound AFP and Ions from lodging between Anti-AFP ELISA under ideal conditions. OurResistance (Ohms)