The ESTs were generated from substractive library with the Suppression Subtractive Hybridization (SSH) between infected and uninfected roots total RNA ( root total RNA in different intervals 1, 3, 7, 12, 24, 48, 72 hours post-inoculation were put into together as the same ratio) of G.babardense variety 7124. The infected pathogens was a kind of highly aggressive defoliating V. dahliae fungus VJ37 from Hebei province in China.