Pseudomonas contamination

Kit (IO20630 at MAINE.MAINE.EDU) wrote:
: Hi! Can anyone give me a suggestion on how to avoid the
: contamination of my YT plates (50ug/ml Ampicillin added)
: after I plated the transformants onto them. I did not have
: this problem before but it occurs quite often recently even
: though I steriled everything. I wonder if there is a vector
: which carrys an antibiotic gene that can kill or suppress the
: growth of Pseudomonas ( I suspect this is the contaminant ).
: Thank you.
: Kit Tam
: UMaine
Find out where the contaminant is coming from. Pseudomonas is not
resistant to sterilization. Different Pseudomonads have different
resistances to different antibiotics and hence you are going to waste
some time finding the right stuff. But if you insist, try Tetracycline at
20 ug/ml
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