Bio- and Chemiluminescence Substrates

...for the most sensitive detection methods

Luminol

Luminol-based Western blot assays use specific antibodies coupled to horseradish peroxidase (HRP) for immunodetection methods. HRP can catalyze the chemiluminescent oxidation of luminol in the presence of hydrogen peroxide. This transformation is used in ultra-sensitive ELISA assays or ECL detection.

Biosynth’s manufacturing process of Luminol (Biosynth Cat.No. L-8600) ensures that highest purity and lot-to-lot consistency is achieved. Therefore it is the product of choice for manufacturers in and high-end immunological assays.

Luciferin

is a substrate for firefly luciferase used for imaging the expression of the luc marker gene and luciferase-fusion conjugates in living cells, tissues and organisms. Luciferin is extensively utilized in reporter gene assays, immunoassays and hygiene monitoring.D-Luciferin Firefly L-8200

AMPPD

AMPPD (or 3-(2'-Spiroadamantane)-4-methoxy-4-(3''-phosphoryloxy)phenyl-1,2-dioxetane) S-7600 is a luminogenic substrate of the dioxetane group for the alkaline phosphatase enzyme. AMPPD is a component of many chemiluminescent immunoassays to detect nucleic acids and proteins. The system is routinely applied in clinical diagnostics.

Structure of 3-(2'-Spiroadamantane)-4-methoxy-4-(3''-phosphoryloxy)phenyl-1,2-dioxetane

Please click here to see all Biosynth luminogenic substates including different Coelenterazines and Lucigenin.

Chemiluminescence and bioluminescence in nature

Chemiluminescence and bioluminescence (the analogous phenomenon in organisms) is observed when a molecule in a chemical reaction changes to an exited state and than emits the excitation energy as a photon. The Chemiluminescence is not an exotic phenomenon, actually a large number of organic materials emit small quantities of photons even at room temperature as a result of oxidation processes. Typically the wavelengths are of emitted light are between 400 - 700 nm, but can extend to the infrared region and UV. The majority of chemiluminescence processes in nature result from redox reactions. An excitation energy of 160 - 320 kJ/mol is required to emit visible light by chemiluminescence. This energy needs to be available in a single reaction step, within a short time and within a relatively small reaction volume, so it is quenched by secondary reactions. (In reaction media fluorophores may be present that are capable to act as energy acceptors. Those molecules may absorb the excitation energy and show fluorescence light as a result).

Biosynth Web Shop

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Please visit our web shop to see the full list of our chemiluminescence products: