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Pro 5-alpha, JM109 and HB101 Competent Cells

Pro 5-alpha can be used for the efficient transformation of unmethylated DNA derived from PCR, cDNA and many other sources (hsdR). The elimination of nonspecific endonuclease I (endA1) enables the highest quality plasmid preparations. The strain is resistant to phage T1 (fhuA2) and suitable for blue/white screening by α-complementation of the β-galactosidase gene. Single-use Pro 5-alpha Competent Cells are available in high efficiency at greater than 109cfu/µg.

JM109 and HB101 Competent Cells are prepared according to a modified procedure of Hanahan.

Pro 5-alpha can be used for the efficient transformation of unmethylated DNA derived from PCR, cDNA and many other sources (hsdR). The elimination of nonspecific endonuclease I (endA1) enables the highest quality plasmid preparations. The strain is resistant to phage T1 (fhuA2) and suitable for blue/white screening by α-complementation of the β-galactosidase gene. Single-use Pro 5-alpha Competent Cells are available in high efficiency at greater than 109cfu/µg.

JM109 and HB101 Competent Cells are prepared according to a modified procedure of Hanahan.

JM109 is a K strain that is recA– and endA– to minimize recombination and improve the quality of plasmid DNA. In addition, the cells carry the F´ episome, which allows blue/white screening. JM109 Competent Cells are available for convenient transformation in two efficiencies: High Efficiency at greater than 108cfu/μg and Subcloning Efficiency at greater than 107cfu/μg.

HB101 cells are recA–, which minimizes recombination events, and contain a restriction-minus background (hsdS20) that allows better representation when cloning methylated DNA. HB101 Competent Cells are suitable for vectors that do not require α-complementation for blue/white screening.

For researchers doing more than one transformation, competent cells are available in standard format (200µl aliquots). For added convenience, single-use competent cells (50µl aliquots) also are offered.

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