Anti-Caspase-3抗体(ab4051)

概述

Caspase-3 is a member of the interleukin-1 ß-converting enzyme family. Caspase-3 is thought to be associated with induction of apoptosis. Caspase-3 is synthesized as inactive 32 kDa proenzyme and is processed during apoptosis generating t-o subunits of 17 kDa and 12 kDa. Caspase-3 stains the epithelial cells of skin, renal proximal tubules and collecting ducts.

Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

IHC-Fr

Use at an assay dependent concentration. PubMed: 24465746

靶标

功能

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp--Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.

组织特异性

Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

序列相似性

Belongs to the peptidase C14A family.

翻译后修饰

Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.

ab4051 staining Caspase 3 in Pig liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in milk) for 20 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

Thank you for contacting us.
I have been in contact with teh lab regarding concnetrations of the antibodies which we spoke of earlier today.
AB4051, rabbit polyclonal to Caspase 3, lot xxx has a concentration of xxxxmg/mL
AB32152, rabbit monoc...

Thank you for contacting Abcam regarding ab833.
I have inquired into the origin of this product. This product is not MIB-1. It is a polyclonal which should react against a number of epitope sites on the target protein.
This product is covered by ...