Search:

MHPH (Maleimide HyNic) Crosslinker

Cat.No.:

S-1009-010

Size:

10 mg

Price:

$290.00

MHPH (Maleimide HyNic) is a maleimide that converts thiols on biomolecules and surfaces to HyNic linker molecules. The S-HyNic (succinimidyl-6-hydrazino-nicotinamide) heterobifunctional crosslinker is fundamental to Solulink's proprietary linker technology.MHPH converts cysteine or thiol groups to HyNic linkers in a single step.The S-HyNic analog reacts with primary thiols on proteins (cysteine residues) introducing a HyNic (6-hydrazinonicotinamide) linker that forms stable covalent conjugates with biomolecules possessing 4FB (4-formylbenzamide) incorporated linkers. The advantages of the MHPH-4FB linker system include reaction specificity, UV-traceability, and the unique control it brings to the entire conjugation process.

S-HyNic crosslinker or its analog is conjugated to biomolecule 1 through primary amines on proteins, oligos, peptides, or surfaces.

S-4FB (succinimidyl-4-formylbenzamide) linker or its analog is conjugated to biomolecule 2 through primary amines on proteins, oligos, peptides, carbohydrates, or surfaces.

HyNic-modified biomolecule 1 is incubated with 4FB-modified biomolecule 2to form a conjugate.

The result is two biomolecules conjugated through a UV-traceable and stable bond (bis-arylhydrazone) with measurable absorbance at 354 nm. Thus any two proteins, regardless of molecular weight, can be efficiently conjugated.

Products and materials supplied by Solulink are for the purchaser's internal research use only. Internal research use only for scientific investigation performed at the purchaser's organization. Solulink material may not be used for any diagnostic, therapeutic or commercial use without a license from Solulink. Commercial purpose means any use for which you are compensated including any use in the manufacture of a product, information or data sale or any resale, repackaging or other transfer for consideration. Commercial licenses are available, please inquire.