BioPlex® 2200 System

Overview

Patented Multiplex Detection Magnetic Bead Technology

BioPlex™ 2200 immunoassays use heterogeneous sets of magnetic 8 µm beads. The beads are infused with varying ratios of fluorescent dyes, creating unique bead sets. Beads within each set are coated with a ligand (i.e., antigen, antibody, analyte, etc.) specific to a particular assay. Bead sets are then mixed together in a single reagent pack, allowing simultaneous detection of multiple analytes from a single sample.

Each microsphere is dyed with a unique combination of 2 colors.

Up to 25 color-coded microspheres (bead regions); can be bound with a unique antigen

Multiplex Chemistry Detection Method

The dual-laser, multiplex flow detection method processes a minimum of 150 individual bead results for each analyte,representing more than 150 assay readings. This enhances the overall quality of each result compared to standardimmunoassays which, rely on a single reading.

Detection 1. A sample of the final reaction mixture is aspirated to the detector.

Detection 2. Bead mixture passes through the detector, where one laser identifies the bead analyte and another measures the analyte concentration.

Detection 3. The system detector performs a minimum of 150 measurements per analyte at the rate of 100,000 measurements per minute.

The BioPlex® 2200 System is the industry’s first fully automated, random access multiplex testing platform. It combines innovative, reliable automation with the diagnostic power of proprietary multiplex chemistry and state-of-the-art eFlex™ software to bring a new level of flexibility and operational benefits to the laboratory.

AutomationThe BioPlex® 2200 improves workflow with random access sampling and priority processing. Performing multiplex analysis on the BioPlex® 2200 is easy: simply load a tray of primary tubes and walk away. The system can automatically process up to 100 samples per hour — for a maximum of 2200 reportable results — with eight hours of walk-away capability. First results are available in approximately 20–45 minutes (assay dependent), with subsequent patient samples completed approximately every 30 seconds.

Patented Multiplex ChemistryBioPlex® 2200 immunoassays use magnetic 8 µm beads, infused with varying ratios of fluorescent dyes to create unique bead sets. Beads within each set are coated with a ligand (i.e., antigen, antibody, analyte, etc.) specific to a particular assay. Bead sets are then mixed in a single reagent pack, allowing for simultaneous detection of multiple analytes from a single sample. The dual-laser, multiplex flow detection method processes a minimum of 150 individual bead results for each assay.

For more than 15 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows high school and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices.

How do I apply for the education discount?

If you are an educator at the high school or college level, visit our Education Discount Policy page to establish an education account number.