"I have been tutoring and mentoring ever since I was in Middle School. Throughout Middle School and High School, I..."

Pedir is used in Spanish when you want to ask for something. For example when you are in a restaurant and you want to ask for a glass of water. Preguntar is used when you are asking a question about something. For example if you wanted to ask the waiter whether or not the water comes with ice or lemon.

Pedir - to ask for an objectPreguntar - to ask a question, to ask for information

"I used to tutor my friends in high school in all kinds of subjects to help them get through the rough parts of their high..."

Pedir means "to ask for something or to order something". I personally would use this in a restaurant setting. Preguntar means "to ask a question". It's easy to differentiate because the word for question in Spanish is "pregunta".

"I have been tutoring for the past 3 years in various levels of Spanish. I have tutored both beginner and intermediate levels..."

Pedir is to ask for something (examples: I am asking for help. Pido ayuda. Ask him for the check. Pídele la cuenta.)Preguntar is to question something (examples: I wonder why this is here? ¿Me pregunto por que ésto está aquí? Ask her if we can take the dog on the train. Preguntala a ella a ver si podemos llevar el perro en el trén.)

Creciente 606S Apartment is located in Fort Myers Beach. Guests benefit from a balcony and a barbecue.

This apartment comes with a kitchen, a seating area, a dining area and a satellite TV.

Guests can make use of the fitness centre. If you would like to discover the area, golfing is possible in the surroundings.

Fort Myers is 16.2 miles from the apartment, while Naples is 19.9 miles from the property. The nearest airport is Southwest Florida International Airport, 12.4 miles from the property.

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To generate a more complete MS/MS network for
B. subtilis
, we combined data sets collected by using a variety of solvents. Specifically, we acquired nanoDESI data by using two solvents: (
i
) a mixture of acetonitrile and 0.05% formic acid in water (ratio of 65:35) for extraction of polar compounds and (
ii
) a methanol:acetonitrile:toluene (50:35:15) mixture for extraction of nonpolar compounds. These experiments enabled the detection of a wide range of molecules within a single mass spectral MS/MS data set including lipids, peptides, small metabolites, and polysaccharides (
SI Appendix
, Fig. S3
). Molecular networking of these data revealed metabolic exchange factors such as the lipopeptides surfactin and plipastatin, the ribosomally encoded peptides SKF and subtilosin, fatty acids and lipids such as diglycosyl glycerides and lysyl-phosphatidylglycerols, and various polysaccharides from the agar medium (
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). For each identified compound, the fragmentation pattern is consistent with the MS/MS reported in the literature (when available), with additional confidence through detection of multiple variations for almost all compounds including H
+
, Na
+
, and K
+
adducts as well as comparison with purified standards (
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) (
20
,
35
⇓
–
37
). By using molecular networking, another unique surfactin analogue was found: two nodes with masses of
m/z
685 (because of variances in spectral quality) were tightly clustered with known surfactins (
m/z
990–1,100). Further inspection of the spectrum revealed that the mass at
m/z
685 is a linear analogue of surfactin with the sequence (I/L)(I/L)VD(I/L)(I/L) (
Fig. 3
A
and
SI Appendix
, Fig. S5
). As the molecular weight of this compound is significantly lower than for any reported surfactin, it would have been much more difficult to recognize the relationship between the MS/MS spectrum of this compound and other surfactins without molecular network visualization.

To highlight the broad applicability of nanoDESI-based molecular networking in microbiology, molecular networks were also constructed for the soil bacteria
Streptomyces coelicolor
,
S. marcescens
ES129 (a common soil organism as well as an opportunistic pathogen that is also associated with eczema),
M. smegmatis
MC2 (a model organism for
Mycobacterium tuberculosis
), and
P. aeruginosa
PAO1 (an opportunistic pathogen of the lung that is also associated with other infections;
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). A variety of structurally and functionally diverse molecules were identified by using these MS/MS networks, which included quorum-sensing molecules like quinolones and peptides as well as glycolipids, antibiotics, pigments, and oligosaccharides (
SI Appendix
, Fig. S4
). Furthermore, it is intriguing to note that a large number of tightly clustered nodes were not annotated, thus highlighting the capability of molecular networks to reveal molecular diversity and potentially novel unidentified compounds.