Figure 3.

PAI-1 expression levels in response to TNFα, TGFβ, or insulin in WRN knocked down fibroblasts. Five synchronized heterozygous fibroblasts cultures (1367CR/1074LF) were transfected
with a molecule of iRNA against WRN messenger (B), or with a scrambled RNA as control (A). PAI-1 inducers (TNFα, TGFβ,
or insulin) were added 24 hours post-transfection and PAI-1 and WRN expression was evaluated by semiquantitative RT-PCR 24 hours after agonist challenge.
Normalized values of mRNA were grouped as mean ± standard error of arbitrary units.
ANOVA was used to test differences across treatments (control, scrambled and iRNA).
* Significant differences compared with scrambled by Tukey test; P < 0.05 and ** P
< 0.01.