AlphaLISA SureFire Ultra Phospho-AMPKα 1/2 (Thr172) Lysate

Extra control lysate for the AlphaLISA SureFire Ultra phospho-AMPKα 1/2 (Thr172) assay. This control is already provided in the AlphaLISA SureFire Ultra phospho-AMPKα assay kit, but can also be ordered separately.

This control lysate is intended for use with the AlphaLISA SureFire Ultra phospho-AMPKα 1/2 (Thr172) assay kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, T175 Flasks of confluent HEK293 cells were stimulated with 11 mM H2O2 for 10 minutes and lysed with 5 mL of 1X Lysis buffer.