In Germany: Registered in accordance with §17c of the German Law on Animal Diseases. Registration No.: BGVV B-332

In Switzerland: Approved by the Federal Veterinary Office (BVET)

Performance

flocktype Salmonella Ab is specific for the detection of antibodies to Salmonella enteritidis and Salmonella typhimurium. Tests were run using the flocktype Salmonella Ab Kit in the Deventer International Proficiency Testing Scheme (PTS) ring trial. Sample sets were from either vaccinated chickens (samples 3, 4, and 5) or from infected chickens (samples 2, 6, 7, and 8). Five positive serum samples were correctly scored as being positive for Salmonella antibodies (samples 3, 4, 5, 6, and 8). Due to the early stage of infection, no specific antibodies could be detected in samples 2 and 7. Sample 1 was correctly scored as negative (see the table Deventer ring trial Salmonella 2011 results).

The kit correctly scored 6 of the 8 samples. Due to the early stage of infection, no specific antibodies could be detected in samples 2 and 7.

Principle

flocktype Salmonella Ab is an ELISA (enzyme-linked immunosorbent assay) with ready-to-use colored reagents. The test plate is coated with a Salmonella-LPS antigen mix. During the sample incubation, Salmonella-specific antibodies bind to the immobilized antigen. The anti-IgY-HRP conjugate detects antibodies bound to the antigen. The color reaction is started by adding the substrate solution and stopped after 10 minutes. The optical density (OD) is measured in a spectrophotometer. The OD values correlate with the concentration of anti-Salmonella antibodies in the sample.

Procedure

Serum and plasma samples can be tested after dilution.

Samples are incubated for 30 minutes with the test plate and if antibodies to Salmonella are present, they will bind to the antigen on the plates. Unbound material is removed by rinsing.