Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

transitional cell carcinoma

Age

80 years

Gender

female

Ethnicity

Caucasian

Applications

The SW 780 line was established in 1974 by A. Leibovitz from a grade I transitional cell carcinoma.

Storage Conditions

liquid nitrogen vapor phase

Derivation

The SW 780 line was established in 1974 by A. Leibovitz from a grade I transitional cell carcinoma.

Clinical Data

80 years

Caucasian

female

Tumorigenic

Yes

Effects

Yes, forms tumors in nude mice

Comments

The patient had preoperative chemotherapy (Thiotepa).

The cells have a reported plating efficiency of 41%.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)

Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

These cells are distributed for research purposes only. The Scott and White Clinic releases the line subject to the following: 1) The cells or products derived from them must not be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, cells or their products. Commercial interests are the exclusive property of the Scott and White Clinic. 2) Any proposed commercial use of these cells or products produced by them must first be negotiated with the Scott and White Clinic, 2401 S. 31 Street, Temple, Texas 76508. Telephone (817) 774-2432