The relationship between physical exercise, either acute or chronic, and metabolic changes resulting from hyperthyroidism has been little studied in the literature. The aim of this study was to analyze the effects of four weeks of aerobic training on the lipid profile of rats with experimental hyperthyroidism. 45 Wistar rats were randomly divided in four groups: Sedentary Control (SC) -administered saline solution during the experimental period and did not exercise (n = 12); Trained Control (TC) - administered saline solution and underwent physical training (n = 11); Sedentary Hyperthyroidism (SH) - induced hyperthyroidism and did not exercise (n=12) and Trained Hyperthyroidism (TH) - induced hyperthyroidism and underwent physical training (n = 10). The aerobic training lasted one hour per day, five times a week, during four weeks. After the training period, the rats were anesthetized in CO2 chamber until their sedation. The blood was collected for total cholesterol, triglycerides, HDL-cholesterol and LDL-cholesterol and serum T3 dosage. Additionally, heart, liver, gastrocnemius muscle and adipose tissue of the mesenteric, retroperitoneal and subcutaneous regions were collected for weighing and triglycerides dosage. Two-way ANOVA, followed by Fisher LSD Post-Hoc was applied for statistical analysis. Lower AGL values were observed in the SH group when compared with SC. The TH group presented lower weight of adipose tissue in the retroperitoneal compared with the SC group. The triglycerides concentrations in the mesenteric, gastrocnemius and retroperotoneal regions were higher in SH group compared with the SC and TC groups. Therefore, it can be concluded that the hyperthyroidism rats presented lipid profile different from the control rats and that aerobic training in rats may have altered the lipid profile of animals with experimental hyperthyroidism compared with the sedentary and control groups.