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CD19 Polyclonal Antibody

CD19 Antibody (PA5-32310)

Immunohistochemical analysis of CD19 using anti-CD19 Polyclonal Antibody (PA5-32310) in Tonsil Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:200.

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* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the
product for use in their own experiment using appropriate negative and positive controls.

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.

Background/Target Information

CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lamda light chains. It is expressed on approximately 10% of normal human peripheral blood cells and approximately 60% of splenic lymphocytes. It is not expressed on granulocytes, monocytes or T cells as defined by CD3 expression. CD19 defines a pan B antigen which is expressed from the earliest stages of B progenitor development, but is lost on terminal differentiation to plasma cells. It may also be present on some early myeloid progenitors, particularly those of the monoblastic type. The CD19 antigen is expressed on approximately 12% of peripheral blood lymphocytes. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.