Protein sharpens salmonella needle for attack

May 15, 2014

This image depicts a section of a Salmonella infected spleen in yellow, red blood cells in red and neutrophils in blue. Credit: University of Basel, Biozentrum

A tiny nanoscale syringe is Salmonella's weapon. Using this, the pathogen injects its molecular agents into the host cells and manipulates them to its own advantage. A team of scientists at the Biozentrum of the University of Basel demonstrate in their current publication in Cell Reports that a much investigated protein, which plays a role in Salmonella metabolism, is required to activate these needles and makes the replication and spread of Salmonella throughout the whole body possible.

The summer months are the prime time for Salmonella infections. Such an infection is caused by the ingestion of contaminated food, for instance ice cream or raw eggs, and can cause severe diarrhea. Salmonella can even cause life-threatening illnesses such as typhoid fever.

For several years, Prof. Dirk Bumann, from the Biozentrum of the University of Basel, has been studying the infection mechanisms of Salmonella. Together with his team, he has discovered that the bacterial protein EIIAGlc is not only responsible for the uptake of nutrients, which was previously known, but also plays a central role in Salmonella colonizing the host organism.

New function discovered for well known protein

Salmonella possesses a sophisticated injection apparatus, the type III secretion system. With this molecular syringe, it injects toxins directly into the host cells. These toxins manipulate host cell processes to create optimal growth conditions for the bacteria in hiding. Unforeseen, Bumann and his team uncovered an important teammate in the infection process, the protein EIIAGlc. The protein was already known for its many functions in bacterial metabolism, such as in the uptake of sugars molecules.

The researchers' attention was attracted by the fact that when EIIAGlc is defective Salmonella completely loses its capacity for intracellular replication and to spread throughout the organism. Further investigations finally brought the scientists from Basel onto the right track. The protein EIIAGlc docks onto the injection apparatus in the bacterium, stabilizes it and finally activates the release of the toxins. "We can clearly demonstrate that the activation of the secretion system is the main function of the protein EIIAGlc, while the many other described metabolic functions play a minor role in the occurrence of illness", says Bumann bringing his findings to the point.

Target molecule for antibiotic treatment

It is estimated that each year about 16 million people worldwide contract a life-threatening Salmonella infection that affects the whole organism. The spread of the bacteria in the host is highly dependent on the functional capacity of the injection system. "In EIIAGlc, we have found a new potential therapeutic target", says Bumann. By inhibiting the protein, one could strategically put the infection apparatus out of action. As this injection needle is primarily found in pathogens, infections could be effectively and specifically fought without harming the natural intestinal microflora.

A research team at the Biozentrum of the University of Basel has discovered an protein family that plays a central role in the fight against the bacterial pathogen Salmonella within the cells. The so called interferon-induced ...

Certain strains of Salmonella bacteria such as Salmonella Typhimurium (S. Typhimurium) are among of the most common causes of food-borne gastroenteritis. Other strains of Salmonella such as S. Typhi are responsible for typhoid ...

Recommended for you

Professor Hyun-Gyu Park of the Department of Chemical and Biomolecular Engineering at Korea Advanced Institute of Science and Technology (KAIST) has developed a technique to analyze various target DNAs using an aptamer, a ...

As an National Science Foundation (NSF)-funded entomologist, Virginia Tech's Paul Marek has to spend much of his time in the field, hunting for rare and scientifically significant species. He's provided NSF with an inside ...

Researchers at the University of Illinois at Chicago and Northwestern University have engineered a tethered ribosome that works nearly as well as the authentic cellular component, or organelle, that produces all the proteins ...

0 comments

Please sign in to add a comment.
Registration is free, and takes less than a minute.
Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.