Autoimmunity Profiling

National facility

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The Autoimmunity profiling facility provides infrastructure and technologies for analysis of the autoantibody repertoire in body fluids and antibody validation using custom designed protein and peptide arrays.

Supported by the resource of more than 42 000 unique human protein fragments generated within the Human Protein Atlas, representing more than 18 000 human protein coding genes, we offer proteome wide screening for autoantibody reactivity on large microarrays in individual or pooled samples, as well as downstream solutions for investigation of autoantibody repertoires in hundreds of patient samples in parallel. Instrumentation and know-how is available for generating customized spotted protein microarrays as well as measurement and analysis of commercially available protein microarrays.

NEWS

A new microarrayer, Arrayjet Super Marathon with JetMosphere Max™, was installed in July 2019 to allow more efficient and accurate printing under highly controlled environmental conditions.

The procurement process for the purchase of a new microarray scanner is also being initiated during the autumn of 2019.

SERVICES

Autoantibody profiling

Epitope mapping

Antibody validation

Infrastructure for commercial protein arrays

Proposed projects are assessed and prioritized according to the following model.

1) Initial assessment by facility:

Technical feasibility and suitability

Required capacity and resources for the project

2) Project prioritization by committee:

Scientific potential

Supporting preliminary data

Significance of facility specific technique for project

Supports facility development (competence and techniques)

APPLICATIONS

Profiling the autoantibody repertoire in plasma, serum or CSF by screening on microarrays of randomly selected antigens. Usually done in the range of the lower hundreds of samples and lower thousands of antigens.

Verification of autoantibody profiles on suspension bead arrays with selected antigens. Usually in the range of lower hundreds of antigens and higher hundreds of samples.

Epitope mapping and off-target analysis of affinity reagents as well as identified autoantibodies on antigen or peptide microarrays.

PERSONNEL

Ronald Sjöberg, Head of Facility

Peter Nilsson, Facility Director

Eni Andersson, Research engineer

Cecilia Hellström, Research engineer

Anna Månberg, Researcher

Elisa Pin, Researcher

Philippa Reuterswärd, Postdoc

David Just, Postdoc

Julia Remnestål, PhD Student

Sofia Bergström, PhD Student

Jennie Olofsson, Research engineer

August Jernbom Falk, Research engineer

SciLifeLab has been created by the coordinated effort of four universities in Stockholm and Uppsala: Stockholm University, Karolinska Institutet, KTH Royal Institute of Technology and Uppsala University.