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TFS Lab User information and training Training you are required to receive includes information on: the physical and health hazards of the chemicals that are stored and used in your work area Your supervisor should provide information on lab-specific procedures and safety (Lab Standard Operating Procedures) the TFS Chemical Hygiene Plan detecting the presence or release of hazardous chemicals protecting yourself management and disposal of hazardous wastes and materials

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General lab rules For the chemicals you are working with, you should be familiar with: the standard operating procedure for using that chemical in your lab (a.k.a. the protocol) the hazards associated with that chemical the PPE required for using that chemical storage requirements waste disposal procedures the procedures to be followed in the event of an emergency

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General lab rules (cont.) Avoid working alone in the lab If you must work after hours or on weekends: make arrangements with others in camp to check in with you periodically. Let someone know you are working alone, and make arrangements to call and check in periodically. avoid conducting hazardous experiments during this time. Do the most hazardous aspects of your work during regular work hours when there are others present.

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Personal hygiene Wash well before leaving the lab, even if it’s only for a short break. Use soap and water, not solvents (which may enhance absorption of the chemical by the skin). Wash immediately whenever any chemical comes in contact with your skin. Flush for at least 15 minutes. Avoid inhalation of chemicals. Do not sniff a chemical in order to identify it.

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Personal hygiene (cont.) No food or drink is allowed in the lab or in chemical storage areas. This includes gum and candy. Application of cosmetic products is not permitted. Use of tobacco products is not permitted in the lab. Smoking is prohibited in TFS facilities. Never pipet any chemical by mouth.

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Housekeeping Keep access to emergency shower and eye wash clear at all times. Keep work areas free of clutter. Keep all aisles, stairs, corridors, and stairwells free of equipment, boxes, chemicals, and debris. Store food and drink in a refrigerator that is designated for that purpose, not in one where chemicals are stored.

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Housekeeping (cont.) Chemical storage areas should be frequently monitored. Inspect for broken, deteriorating, or leaking containers. Ensure that all containers are clearly labeled with the name and hazard of the chemical (e.g. “Hydrochloric acid, corrosive” or “Ethanol, flammable”). Chemicals must be put back into their proper storage location at the end of the day. At the end of a work day, any chemical in an unlabeled container should be considered a waste and disposed of appropriately.

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Housekeeping (cont.) Broken glassware, microscope slides, Pasteur pipets, etc. must be collected in a puncture resistant container and labeled with the words “Broken glass disposal”. Waste collection containers Must be clearly labeled with the name(s) of the chemical(s) and the hazard(s) they present Must be compatible with the chemical Must be intact, with a lid or cap that can be sealed

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Personal protective equipment General clothing requirements Long pants and long-sleeved shirts should be worn while working with chemicals. Avoid wearing excessively loose or baggy clothing. Lab coats and aprons Lab coats should be worn while working with chemicals. Chemical-resistant (Tychem®, Saranex, or similar fabric) coats are recommended for particularly hazardous chemicals. Contact UAF EHS&RM for more information on specific chemicals ( ). Full-length rubber, neoprene, or plastic aprons are recommended when there is a risk of a splash or spill.

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PPE (cont.) Gloves Use gloves that are appropriate for the material you are working with Heat-resistant for handling hot items, cryoprotective for handling liquid nitrogen Chemical resistant—material depends on type of chemical being used  Consult your UAF EHS&RM for information  provides excellent information Understand the limitations of gloves Chemicals do break through the material over time—do not reuse disposable gloves Change gloves often

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PPE (cont.) Eye protection Use safety glasses when there is a chance of a small splash to the eyes, such as when opening a bottle or tube. Use safety goggles when using a highly caustic chemical, or when using large volumes (1 L or more) of a chemical. Use full face shields when working with very large amounts of hazardous chemicals, or when you need to protect your entire face (removing items from liquid nitrogen, using a transilluminator)

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PPE (cont.) Eye protection (cont.) Regular prescription eyeglasses are not considered effective eye protection. Wear goggles over the glasses. Care must be taken when wearing contact lenses in the lab. Goggles or safety glasses must be used.

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Chemical hazards Chemical hazards fall into 4 main categories Flammables Corrosives Reactive chemicals Health hazards Some chemicals are not generally regarded as hazardous under normal conditions of use These include chemicals like sodium chloride, potassium phosphate, sucrose, Tris buffer, glycerol, etc. These chemicals are color-coded green, orange, or gray. These chemicals have a hazard class rating of 2 or less in any category (flammability, corrosivity, health, reactivity).

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Chemical hazards: flammables Storage and labeling Flammables are color-coded red DOT hazard class 3 Must be stored in a flammable cabinet, away from sources of heat and/or ignition (sparks) Handling Never use an open flame to heat flammable materials. Use hot water bath, oil bath, heating mantle, etc. Transfer from 5 gal. containers to smaller containers in a fume hood or approved flammable liquid storage room. Handle only in areas free of ignition sources. Do not transfer Class I flammable liquids in an exit way. Flammable

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Chemical hazards: corrosives Definition: Cause rapid erosion and destruction of building materials or metals Burn, irritate, or cause destruction to organic tissues such as skin, eyes, lungs, and stomach DOT hazard class 8 Know the location and proper use of spill kits in your lab. Never use combustible organic materials (such as paper, sawdust, or rags) to clean up spills.

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Bases Are color-coded Solids may be stored on shelf in lab, away from other chemicals and below eye level Liquids may be stored in a cabinet, away from acids  Use secondary containment for liquids Chemical hazards: corrosives Classification, labeling, and storage: Acids Are color-coded Store liquids and solids in corrosive cabinet, separate from bases Keep below eye level Corrosive

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Chemical hazards: corrosives Handling Never pour water into acid. Always add the acid to the water. Add acid slowly, with stirring. Open bottles of acid slowly and carefully. Wear PPE (gloves, goggles/shield, apron) to protect against splashes. Conduct all work with concentrated acids and bases in a chemical fume hood. Check location of eyewash and shower prior to beginning work. Some acids require special handling: Example: hydrofluoric acid, picric acid, perchloric acid Contact UAF EHS&RM for assistance ( ).

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Chemical hazards: corrosives Wastes Never mix acid wastes with solvents or metal- containing solutions. Never dispose of acids or bases down the drain. Empty bottles should be triple-rinsed with water (collect rinse solutions in waste acid container) before disposal or reuse of the bottle.

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Chemical hazards: reactives and oxidizers Definition: Chemicals which will “vigorously polymerize, decompose, condense, or become self-reactive under conditions of shock, pressure or temperature” (29 CFR (b)) Chemicals which react violently when exposed to air or water Oxidizers cause other substances to burn more easily DOT hazard class 5 Explosive Oxidizer

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Physical hazards: Compressed gases Compressed gas cylinders must be restrained in an upright position in the lab. Caps must be in place when cylinder is not in use. Make sure regulator and supply lines are in good condition. Never use rigid plastic tubing, which can shatter if the pressure limits are exceeded. When turning on the gas Ensure that the flow valve is open (so there will be no pressure in the supply line). Adjust flow valve only after you have opened the regulator. Turn your head away from the tank. Compressed gas safety training is available. Call UAF EHS&RM at

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Physical hazards: Electrical equipment Always inspect electrical cords prior to use. Do not use if they are cracked or have exposed wiring. Never use electrical appliances near water. Make sure hands are dry when unplugging a cord. Do not overload outlets. Avoid excessive use of extension cords. Never override the safety features on electrical equipment.

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Physical hazards: Lasers If you work with lasers in your lab, your supervisor should provide you with specific operating procedures and safety information. Laser Safety training is available through UAF EHS&RM ( ).

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Physical hazards: Thermal hazards Thermal hazards include both hot and cold objects. Hot items: Use heat-resistant gloves when handling hot items. Use caution when heating liquids on hot plates. Use a stir bar or Boil-Eezers to ensure even heating of the liquids (to prevent superheating and boil-overs) Never leave hot plates unattended. Bunsen burners Inspect tubing prior to using the burner. It should not have cracks, and should fit tightly to the burner and to the gas spigot.  Be alert to gas leaks along the tubing—these can ignite. Stand back from the burner when lighting the gas.

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Physical hazards: Thermal hazards Cold Liquid Nitrogen (LN2) Wear insulated or cryoprotective gloves when accessing LN2.  Note: cotton mittens are not sufficient. Splashes of LN2 can easily penetrate the mittens, causing frostbite and serious injury. Wear face shield or splash goggles to protect face and/or eyes from splashes Use caution when adding items to LN2. Rapid addition of items can result in splashes to the face and hands. Tubes that have been stored in LN2 should be thawed behind a shield.  Sometimes LN2 leaks into the tube during storage. The nitrogen will rapidly expand upon warming, causing the tube to shatter. Liquid Nitrogen training is available ( ).

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Ventilation Chemical fume hood Keep sash at or below “maximum sash height” posted on fume hood. Fume hoods are tested annually at TFS. If your fume hood does not seem to be working properly, contact TFS safety/hazmat staff. Conduct all work at least 6” inside fume hood. This reduces the chance that vapors will backwash out of the hood due to air movement. Do NOT store chemicals in a fume hood. Do NOT block the vents at the back of the fume hood. If a large object must be placed inside the hood (e.g. a water or acid bath, muffle furnace), elevate it slightly so that air can flow under the object.

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Emergency Response Fire If your clothing catches on fire, drop and roll to put out the flames. Immediately notify your supervisor of the incident. Chemical spill on clothing Get to the safety shower immediately and remain there for at least 15 minutes. Remove contaminated clothing while in the shower. Notify your supervisor of the incident.

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Emergency Response Chemical splashes to the eye Immediately go to the eye wash station and flush eyes with water for at least 15 minutes. Hold lids open to allow water to reach all surfaces of the eye and eyelids. Seek medical attention and notify your supervisor of the incident immediately. Burns Cool the burn using cold running water. Seek medical attention and notify your supervisor of the incident immediately.

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TFS Lab Safety Quiz For quiz –copy and paste URL into web browser: powerpoin/tfs-lab-safety-quiz/ If you are unable to copy and paste the URL, please write the URL address on a piece of paper, open new web browser, and type in the URL. Complete quiz, click the “send quiz to TFS operations” button. Once the quiz has been received and graded you will receive an confirmation. You must score an 85% or higher (13 correct out of 15) on this quiz in order to attain a passing grade.