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FOX LAKE, Ill. -- The head of the investigation says he remains optimistic, even though this investigation is proving to be complicated.

For now, it’s two steps forward, one step back.

The update came less than 24 hours after the Fox Lake Police Lieutenant Charles Joseph Gliniewicz was laid to rest.

As of today, there have been 50 DNA samples taken from people who had contact with the Fox Lake officer and investigators are following up on more than 185 leads in the case.

A week ago today, Lt. Gliniewicz called in for backup as he chased, he said, three men in a heavily wooded area in Lake County.

When officers arrived, the lieutenant was shot and killed. A massive manhunt turned up nothing. Video from the area pinpointed three people, who police found, questioned and determined they had no link to the case. Now it’s DNA transfer evidence, found at the crime scene, that could be the next big break.

There’s also been a major shakeup in leadership within the Fox Lake police department since last week. Reporters were told today that the death of Lt. Gliniewicz has nothing to do with that change. Investigators say they are still awaiting a final autopsy report on the lieutenant as well as more information on that transfer DNA found at the scene.