Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Claudin 2 (CLDN2)
No significant cross-reactivity or interference between Claudin 2 (CLDN2) and analogs was observed.

Description:

LS-F6856 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Mouse CLDN2 / Claudin 2 in samples of Cell Lysates and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CLDN2 / Claudin 2 as low as 0.055 nanograms per millilter.

Custom Kits:

All LSBio Custom kits have been predesigned and all necessary components, including antibodies and standards have been identified. Upon receiving a custom kit order, the kit will be assembled and quality control tested before being shipped out. Kit assembly and testing typically takes 4 to 6 weeks. In most cases the final kit is based on the Sandwich assay principle, with a few being Competitive EIA based. Specifications such as Range, Sensitivity, and Precision are defined upon completion. In the event that the custom kit cannot be successfully developed with 6 weeks of the order date, the customer will be notified and the order canceled at no cost.

Sample Type:

Cell Lysates, Tissue Homogenates

Format:

96-Well Strip Plate

Detection:

Colorimetric - 450nm

Detection Range:

0.156 - 10 ng/ml

Sensitivity:

0.055 ng/ml

Assay Time:

4.5 h

Precision:

Intra-Assay: CV<10% Inter-Assay: CV<12%

Quality Assurance:

Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping. ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Kit Components:

Coated 96-well Strip Plate

Adhesive Plate Sealers

Standard (Lyophilized)

Sample Diluent

Detection Reagent A (Green)

Detection Reagent B (Red)

Assay Diluent A

Assay Diluent B

TMB Substrate

Stop Solution

Wash Buffer (30x concentrate)

Restrictions:

For research use only.

About CLDN2 / Claudin 2

Description: CLDN2 / Claudin 2 belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.