Ray,
I think the same as you do and would like to know what other people
in addition to Gayle are thinking. I also thought that when the cells were
fixed they cytokines should remain in there respective areas and should be
detectable without permeablization. I suppose that if you were to cut a
section and some of the cells were not sectioned through that
permeabilization would enhance staining. One of the ideas that seems to
drive my work is that if it isn't broken son't fix it. So if a stain is
developed for cell suspensions PI's want the EXACT same protocol used on
sections.
Cynthia Favara
NIAID/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
PH: 406-363-9317
FAX: 406-363-9286
e-mail: CF98d@nih.gov <mailto:CF98d@nih.gov>