Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age

2.5 years

Gender

female

Ethnicity

Caucasian

Storage Conditions

liquid nitrogen vapor phase

Karyotype

number = 48; trisomic for X; trisomic for a group G chromosome; Down syndrome

Clinical Data

female

Caucasian

2.5 years

Virus Susceptibility

Human poliovirus 1

Comments

The cells have a finite life expectancy of 30 serial passages from the tissue of origin.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37.0°C

STR Profile

Amelogenin: X

CSF1PO: 11,13

D13S317: 11,13

D16S539: 13,14

D5S818: 12,13

D7S820: 10

THO1: 8,9

TPOX: 8,11

vWA: 18,19

Isoenzymes

G6PD, B

Name of Depositor

CS Stulberg

Deposited As

Homo sapiens

Passage History

The cells have a finite life expectancy of 30 serial passages from the tissue of origin.

Year of Origin

October, 1963

Permits

These permits may be required for shipping this product:

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.