The decision adds to the stack of legal troubles facing zoo owners Gus and Betty White following the most recent Mississippi Department of Wildlife, Fisheries and Parks raid of the U.S. 49 facility in Collins in January 2012.

During the raid, state wildlife officials sedated and seized three tigers, two leopards, three cougars, two wolf hybrids and a macaque monkey. At the time, a Humane Society representative said the zoo didn't have the proper state permits to house the animals, which she said were living in poor conditions.

Betty White told The Hattiesburg American that she plans to appeal the decision made earlier this month within her 30-day timeframe.

USDA moved for license revocation after a large number of animals died at the facility, including at least one tiger, a lion, a leopard, a cougar, a wolf and a dingo in a two-year period from 2010-2012.