Results: The proposed method shows a good linearity in the concentration range of 60–900 μg/ml for NTP and 0.1–1.5 μg/ml for PLS under optimized conditions. All the precision and recovery results are in between 98 and 102%. In the entire robustness conditions, percentage of relative standard deviation is <2.0%. Degradation has minimum effect in stress condition and solutions are stable up to 24 h. This method is validated different parameters such as precision, linearity, accuracy, limit of detection, limit of quantification, ruggedness, robustness, and forced degradation study were determined according to the International Conference of Harmonization (ICH) Q2B guidelines.

Conclusion: All the parameters of validation were found to be within the acceptance range of ICH guidelines. Since there is no HPLC method reported in the literature for the estimation of NTP and PLS in pharmaceutical dosage forms, there is a need to develop quantitative methods under different conditions to achieve improvement in sensitivity, selectivity, etc. Hence, the author has attempted to develop a validation and forced degradation for simultaneous quantification of NTP and PLS.

The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.

Online ISSN: 2455-3891Print ISSN: 0974-2441

Journal Metrics

Source Normalized Impact per Paper (SNIP): 0.492

Source Normalized Impact per Paper (SNIP):2017: 0.492SNIP measures contextual citation impact by weighting citations based on the total number of citations in a subject field.

Impact per Publication (IPP): 0.588

Impact per Publication (IPP):2016: 0.588The Impact per Publication measures the average number of citations received in a particular year by papers published in the journal during the three preceding years.

SCImago Journal Rank (SJR): 0.22

SCImago Journal Rank (SJR):2017: 0.22SJR is a prestige metric based on the idea that not all citations are the same. SJR uses a similar algorithm as the Google page rank; it provides a quantitative and a qualitative measure of the journal’s impact.

Cite Score: 0.49

Cite Score:2017: 0.49CiteScore metrics are a new standard to measure serial citation impact.