DESIGN:

Male C57BL/6J mice were fed a semipurified diet containing DHA or the control lipid. Serum, skeletal muscle, epididymal fat pads and liver were collected after 62 and 118 days of feeding. Metabolites, genes and gene products associated with the ECS, glucose uptake and metabolism and inflammatory status were measured.

CONCLUSIONS:

This study provides evidence that DHA feeding altered ECS gene expression to reduce CB1 activation and reduce fat accretion. Furthermore, the DHA diet led to higher expression of genes associated with glucose use by muscle in mice, and reduced those associated with systemic inflammatory status.

CB1, CB2, GLUT4 and INSULIN-R expression in mouse quadriceps and epididymal fat pad after 62 and 118 days of feeding AIN 93M semipurified diets: western blot comparison between the treatment (DHA) and control groups. Values are mean±s.d. for n=9 measurements. Differences between diet groups and time were compared by t-test. * represents differences between diet groups; † indicates significant difference over time within the same diet group.

C57BL/6J mice fed a semipurified diet containing DHA showed higher levels of DHA and eicosapentaenoic acid (EPA) but lower AA in muscle and adipose tissues compared with those fed the control diet. Furthermore, the mice given DHA showed higher blood levels of DHEA and lower levels of AEA, 1-AG and 2-AG. The DHA diet also resulted in higher CB1 and CB2 protein levels and gene expression for these receptors in muscle compared with those given the control diet. Moreover, these changes resulting from DHA feeding also improved gene expression favorable for glucose uptake in muscle and lower glucose uptake by adipose associated with lower CB1 in mice.