Abstract:Background: Tuberculosis is a global infectious health problem. The association between tuberculosis (TB) and hypocalcaemia is well recognized. The reported incidence of hypocalcaemia in TB varies widely between countries, probably because of differences in the vitamin D and calcium intake, the amount of sun exposure, the extent of disease and the criteria for hypercalcaemia.Objective: The aim is study is To compare serum calcium, phosphorus, vitamin-D and alkaline phosphatase, level in pulmonary tuberculosis patients with normal healthy controlMethodology: A Hospital based Case control study conducted at Department of Tuberculosis and Respiratory medicine, Institute of respiratory diseases SMS Medical College, Jaipur, Rajasthan during the year 2014 – 2015.The present study includes total 164 participants among them 82 adults newly diagnosed pulmonary tuberculosis patients of both genders of age group 18-80 yr (Group A) and 82 age and sex matched healthy control. (Group B). Blood sample was collected from all participants to measure complete blood count (CBC), ESR, S.Calcium, S.Phosphorus, S.alkaline phosphatase and Vitamin D. sputum sample was also collected for AFB detection. Obtained results were analysed statistically to determine the difference of significance by calculating p value. Results: The Mean level of S.Calcium(mg/dl),S.Phosphorus(mg/dl),S.alkaline phosphatase(U/L) and Vitamin D(ng/ml) in case group is group is 7.74+ 2.77, 3.52+ 1.07, 118.95+18.95, 19.33+ 5.88 while in control group it is 9.27+2.11, 3.92+1.14, 63.16+19.40, 46.23+12.63 Respectively. Conclusion: In conclusion, serum calcium and phosphorus significantly decreased in pulmonary tuberculosis. While the cationic levels decreased to hypocalcemic range, the decrease in phosphorus was within normophosphatemic range. High prevalence of vitamin D deficiency was found among newly diagnosed TB patients.