Stable Transfections - do you NEED to linearise the plasmid? (Mar/31/2005 )

hi all

this may be a silly question, but i was wondering whether you need to linearise your plasmids before transfecting them into eukaryotic cells for stable transfection. i've heard arguements for and against. what do you guys do?

thanks

-ros-

hithere was a discussion here about linearize plasmid before transfection. I think this is not a necessity due to the fact you further select cells that are ok. Moreover, linearize a plasmid do not prevents from an other cut in the DNA in the cell before integration...