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Description

Applications

β-Glucuronidases are routinely used for the enzymatic hydrolysis of glucuronides from Serum plasma and other fluids prior to analysis by enzyme immunoassay, mass spectrometry, gas chromatography, high performance liquid chromatography, or other means.β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

Product Description

Beta-glucuronidases are members of the glycosidase family of enzymes that catalyze breakdown of complex carbohydrates. Human β-glucuronidase is a type of glucuronidase that catalyzes hydrolysis of β-D-glucuronic acid residues from the non-reducing end of mucopolysaccharides such as heparan sulfate. In human gut β-glucuronidase converts conjugated bilirubin to the unconjugated form for reabsorption.

Biochem/physiol Actions

β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.β-Glucuronidase from Helix pomatia is a mixture of enzymes. Inhibitors: D-glucuronic acid, D-galacturonic acid, D-glucaro-1, 4-lactone

β-glucuronidase also can be used as reporter gene to monitor gene expression in mammalian and plant cells. Monitoring β-glucuronidase activity through the use of a GUS assayallows determination of the spatial and temporal expression of the gene in question. Its Inhibitors are D-glucuronic acid, D-galacturonic acid and D-glucaro-1,4-lactone. Following are the Substrates: 5-Bromo-6-chloro-3-indolyl b-D-glucuronide 6-Bromo-2-naphthyl b-D-glucuronide 5-Bromo-4-chloro-3-indolyl b-D-glucuronide sodium salt tablet 8-Hydroxyquinoline glucuronide 4-Methylumbelliferyl b-D-glucuronide 4-Nitrophenyl b-D-glucuronide.