In this study, the most effective incubation methods for biocellulose production were examined in various mode of bioreactor, and then on the basis of the results, the bioreactor with rotating-disk has been designed. This is composed of horizontal pyrexcylinder of 20cm long and 15cm diameter, and stainless-made side plates equiped with one side of motor-drive, openings for culture medium and aeration, and with another side of pH and oxygen electrodes, two valves. Warmed water is passed through a thermojacked under the cylinder maintained at 28(]SY+-[)1ﾟC.pH in culture medium was automatically controlled by 1% NaOH aqueous solution. Aeration was done only into gas-phase by the filtered-air. Initial volume of the medium is 1800ml, that is half volume of the cylinder. Stainless mesh-disk from 6 to 16mesh of 14.4cm diameter were used as a supporter for bacterial cells. 60ml of preculture was inoculated into the reactor horizontally rotating 2-5rpm at 28ﾟC,and then incubated for 6days with aeration of 2000ml/min. The incubation was repeated 9times. Then total yealds of biocellulose has been examined for rotating speed and number of the disk, or pH control and aeration conditions. The most effective incubation conditions for the reactor of biocellulose production were as follows. 14mesh stainless disk was the most effective supporter for bacterial cells. Although the bioreactor produced about the same amount of biocellulose with respect to rotating speed of the disk, 30 and 50rpm, in the case of 50rpm incubation, there was some scattering of biocellulose too fast to retain on the disk. So the optimum rotating speed is 30rpm. Aeration into gas-phase would contribute to produce more biocellulose and to enhance physical properties of the biocellulose, especially, to increase in Young's modulus of the sheets.