Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 450

Can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and PI, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized and stained for intracellular antigens without loss of staining intensity.

Description

Fixable Viability Dyes may be used to label cells from all species

Fixable Viability Dye eFluor™ 450 can be excited by the violet (405nm) laser line and has a peak emission of 450nm that can be detected using a 450/50 band pass filter (equivalent to eFluor™ 450 or Pacific Blue™)

For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye

If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute. After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye.

Fixable Viability Dye eFluor™ 450 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO. It should be protected from light and moisture

Store at −70°C with dessicant

It may be freeze-thawed up to 20 times

Allow vial to equilibrate to room temperature before opening

Staining may be done before or after surface staining

Cells may be cryopreserved after staining with no adverse effect on staining intensity of dead cells after thawing