Our project aims to construct a chain-reactive molecule-releasing system in a spontaneous manner. Delivering drugs at appropriate places and controlling the quantity of released drug is very important to develop effective DDS. Our system makes these possible.<br>

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The system consists of two sub-systems: “egg-type initiator” and “chain-reactive burst”. <br>

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The “egg-type initiator” releases trigger DNAs under a certain condition like warming up to body temperature. The trigger DNAs hybridize to aptamer DNAs on liposomes, and the hybridization causes liposomes collapse. The collapsed liposomes release drugs and new trigger stored inside. These processes are the “chain-reactive burst”, and achieve sequential and exponential release of drugs. <br>

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To realize the egg-type initiator, trigger DNAs and chelate compounds are encapsulated in temperature-sensitive liposomes, and the liposomes are encapsulated in alginate hydrogel. Increasing temperature disrupts the liposomes, and then chelate compounds inside the liposomes melt the gels. Collapse of liposomes is realized by rapid deformation of liposomes mediated by hybridization between trigger and aptamer DNAs. <br>

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Our system will propose a new place and quantity controlling system of drug release by liposome-based drug delivery systems.<br>

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We are aiming at constructing a chain-reactive molecule-releasing system in this project. <br>

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In living organisms, signal transduction is widely used in many situations. <br>

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Here we construct a signal transduction-like system through an approach of DNA nano-engineering. <br>

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In our project, trigger DNAs and payload molecules are encapsulated in a liposome. The trigger DNA is specially designed to collapse the liposome when some initiation stimulus is applied. Once one of the liposomes collapsed, the trigger DNAs are released from the liposome and that break other liposomes in chain-reactive fashion. This mechanism of “chain-reactive burst” enables us to release a large amount of payload molecules all at once, driven by a faint trigger signal. <br>

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We believe this system offers a fundamental technique of signal transduction/amplification of various kinds of chemical or physical signals, applicable to Drug Delivery System, molecular computers and so on.<br>

Revision as of 22:06, 26 October 2013

Abstract

We are aiming at constructing a chain-reactive molecule-releasing system in this project.
In living organisms, signal transduction is widely used in many situations.
Here we construct a signal transduction-like system through an approach of DNA nano-engineering.
In our project, trigger DNAs and payload molecules are encapsulated in a liposome. The trigger DNA is specially designed to collapse the liposome when some initiation stimulus is applied. Once one of the liposomes collapsed, the trigger DNAs are released from the liposome and that break other liposomes in chain-reactive fashion. This mechanism of “chain-reactive burst” enables us to release a large amount of payload molecules all at once, driven by a faint trigger signal.
We believe this system offers a fundamental technique of signal transduction/amplification of various kinds of chemical or physical signals, applicable to Drug Delivery System, molecular computers and so on.