Product details

Product details

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Immunogen

Extract of pigmented melanoma metastases from lymph nodes.

Clone

HMB45

Isotype

IgG1

Fragment

Light chain,Chain kappa

Specificity

This antibody reacts with a neuraminidase sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. It reacts with junctional and blue nevus cells. Non-melanocytic cells are negative.

Target details

Target details

Application Details

Application Details

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Application Notes

IHC: This antibody may be diluted to a titer of 1:10-1:50 in an ABC method. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Enzymatic antigen retrieval may increase staining intensity when using formalin fixed paraffin embedded tissue.