"I used Applied Biosystems and Qiagen One-Step PCR Kits the first time I ran an experiment to quantify mRNA from primary rat splenocytes. I preferred the Applied Biosystems Kit because the directions were clear, there were fewer reagents that needed to be incorporated into the master mix and the results were more consistent between replicates."

The TaqMan® One-Step RT-PCR Master Mix Reagents Kit, which combines the reverse transcription and amplification steps, permits excellent results even when quantifying minute amounts of RNA. Set-up is quick, easy, and minimizes risk of sample crossover and contamination, making this kit well-suited for all high-throughput applications.

• Optimized for high sensitivity and yield • Combined RT (reverse transcription) and amplification for convenience and ease-of-use • High-quality manufacturing for robustness and reproducibility