In article <pxpst2-3011981308270001 at pelli.pathology.pitt.edu>,
pxpst2 at unixs.cis.pitt.edu (Peter) wrote:
> In article <73ui0k$eer$1 at nnrp1.dejanews.com>,
>theodorn at medlib.georgetown.edu wrote:
>> > It's funny, but when I used to do a lot of metabolic labelling experiments, I
> > used good old PPO/DMSO, which seems to be out of fashion these days, but I
> > never had a gel break on the dryer (even 15% gels). They seem pretty tough
> > after the PPO is precipitated into the gel; you can pretty much throw them
> > around.
>> But there is a good reason why this techinique has lost favor. The
> chemicals are rather nasty.
>> Peter Pediaditakis
>> --
> Peter
>> " Don't you eat that yellow snow
> Watch out where the huskies go"
> FZ
>
Well, I hate to start another thread like the one we had on the toxicity of
acrylamide, but... is it really true? I have often heard that PPO is supposed
to be nasty, but without any supportive evidence. OTOH, consider this MSDS
data..
(from http://www.packardinst.com/msds/msds139.htm)
OMNIFLUOR®
1. Identification of the Substance/Preparation and of the
Company
Company Name:
Packard BioScience B.V.
Chemical Operations
P.O. Box 9403
9703 LP Groningen
The Netherlands
Tel: (31) 50-5413360
Fax: (31) 50-5422292
Emergency Telephone: (31) 50-5413360
Chemical Family: Mixture of aryloxazole and aromatic
hydrocarbon
2. Composition/Information on Ingredients
Material CAS Number Concentration
2,5-Diphenyloxazole (PPO) 92-71-7 97-99%
p-Bis-(o-methylstyryl)-benzene (bis-MSB) 13280-61-0 1-3%
3. Hazards Identification
Symbol -
Special Risk for People and the Environment
None
^^^^
HMIS Hazard Code Ratings
Health 1
Flammability 0
Reactivity 1
Personal Protection B
<snip>
11. Toxicological Information
2,5- Diphenyloxazole
Eye: Irritating
Skin: Non-irritating
Toxicokinetics: Non-accumulative
Mutagenicity: Non-mutagenic
^^^^^^^^^^^^^
Carcinogenicity: Non-carcinogenic
^^^^^^^^^^^^^^^^
Teratogenicity: Non-teratogenic
^^^^^^^^^^^^^^^
(emphasis is mine)
-----------------------------------------------------------------------
Nick Theodorakis
-----------== Posted via Deja News, The Discussion Network ==----------
http://www.dejanews.com/ Search, Read, Discuss, or Start Your Own