Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

cutaneous T cell lymphoma

Age

61 years adult

Gender

male

Ethnicity

Caucasian

Storage Conditions

liquid nitrogen vapor phase

Derivation

The line was isolated from a patient who was negative for anti human T lymphotropic virus type I (HTLV-I) antibody, and no HTLV-I sequences were detected in the cultured cells by Southern analysis.

Clinical Data

61 years adult

The line was isolated from a patient who was negative for anti human T lymphotropic virus type I (HTLV-I) antibody, and no HTLV-I sequences were detected in the cultured cells by Southern analysis.

Caucasian

male

Antigen Expression

CD2; Homo sapiens, expressed

CD3; Homo sapiens, expressed

CD4; Homo sapiens, expressed

CD8; Homo sapiens; negative

CD25; Homo sapiens; negative

CD30; Homo sapiens, expressed

HLA-DR; Homo sapiens, expressed

Receptor Expression

interleukin 2 (IL-2) beta chain, expressed

HeLa Markers

N

Comments

Although HH cells lack the alpha chain of the IL-2 receptor (IL-2R alpha), they do possess a functional p70 beta chain (IL-2R beta) of the IL-2 receptor complex.A culture submitted to the ATCC in June of 1992 was found to be contaminated with mycoplasma, and was cured by treatment with Ciprofloxacin.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.

HH is a mature T cell line derived in 1986 from peripheral blood of a patient with aggressive cutaneous T cell leukemia/lymphoma. The line was established from blood taken after extensive chemotherapy.

Permits

These permits may be required for shipping this product:

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

HH is a mature T cell line derived in 1986 from peripheral blood of a patient with aggressive cutaneous T cell leukemia/lymphoma. The line was established from blood taken after extensive chemotherapy.