Immersion Oils

Cargille Immersion Oils

Cargille Immersion Oils are in full accord with the FDA requirements.
To date, no other immersion oils meet the FDA criterias.
Cargille Immersion Oils are PCB (polychlorinated biphenyls) free.

Immersion Oil Selection Guide

Standard Light Microscopy
Type A & B, with viscosities of 150 and 1250 cSt (centistokes),
respectively, meet these requirements. The greater the gap between
cover glass and objective, or condenser and slide, the more desirable
high viscosity becomes. Type A, with 150 cSt, reduces any tendency to trap air bubbles, thus avoiding image degradation.
Type B, with 1250 cSt, is thick enough for viewing multiple slides with one application, thus saving time during batch
processing. Cargille Immersion Oils A, B, NVH, OVH and 300 can be blended for intermediate viscosities.
Cargille Immersion Oil Specifications

Inverted or Inclined Microscopy and Projection Instruments
Use immersion oils type NVH or OVH. The greater the gap between the objective and the cover glass or between the
microscope slide and the objective, the more necessary high viscosity becomes. The very high viscosities of type
NVH at 21,000 cSt and Type OVH at 46,000 cSt give excellent results for these applications.
Cargille Immersion Oil Specifications

Elevated temperatures (>23° to 37°C)
Use type 37. Elevated temperatures can be caused by sub-stage illuminators, environment or other causes. Developed
for working at human body temperatures there is no image degradation above the standard calibration temperature of
23C. Can be blended with type B to place the temperature of calibration proportionally between 23° and 37°C. Type B
and type 37 both have a viscosity of 1250 cSt at their respective calibration temperatures.
Cargille Immersion Oil Specifications

Why do Cargille Immersion Oils Meet the DIN 58 884?
(Deutsches Institut Für Normung e.V.)
Refractive index, dispersion and temperature standardizations
are the key specifications for optical clarity and high resolution.
(Viscosity and other physical properties are directed toward
specific applications and convenience of the user.)
Immersion oil specifications must meet the design requirements
of old instruments and become the design criteria for new instruments.
Without these specifications, it would be much harder for the
microscopist to find the proper oil for his or her particular
instruments' requirements and optical imagery would suffer.
The specifications adopted by DIN resulted from their study of
instrument requirements of many manufacturers. The proposed specifications
were submitted for review, with Cargille Laboratories serving
as liaison for the American manufacturers.
The present trend toward tighter specification for optical properties
is under a program of the International Standards Organization
(ISO).

Unique Applications - Special Properties
Because of widespread availability, consistent quality, and close
specifications, Cargille Oils are used as:

A temporary mounting media

Fluid mounts (permitting specimen rotation)

Calibration liquids

Optical coupling fluids

Combination protective coating and examination fluid

Transparency medium for translucent materials

Filter clearing agent (as recommended by Millipore Corp.)

Important Comments
Cargille Immersion Oils are supplied as a microscopy accessory
by American Optical and Bausch & Lomb, and accepted for use
by all foreign microscope manufacturers. Cargille Immersion Oils
are synthetic hydrocarbons, natural petroleum derivatives that
contain no solvents and no PCB's. These oils are stable, non-drying,
odorless, safe for lens and handling, and easily removable. MSDS
sheets are available.

Citifluor AF87

Citifluor AF87 is a medium viscosity immersion oil with a refractive index of ~1.52 at room temperature (20°C).
This oil is ideal for confocal laser scanning microscopy and FISH label with DAPI and Cy dyes. Citifluour AF87 should be stored at room temperature. Low temperature may cause sediment to develop and reduce performance.
SDS (47KB PDF)

Prod #

Description

Unit

Price

Order / Quote

19476-A

Citifluor Non-Flourescent Immersion Oil, AF87, 10 ml

each

$72.00

Qty:

Box and Booklet shown

PELCO® Optical Lens Tissue

Fully comparable to Ross optical lens tissue

The PELCO® Optical Lens Tissue is a high quality
optical lens tissue for lenses, optical instruments, electron microscopy and similar
applications. PELCO® Optical Lens Tissue is fully
lab tested and is comparable to the Ross lens tissue paper in regards to structure, softness,
properties and cleaning capabilities. It is a direct replacement for Ross lens tissue and
fully compatible for all applications. The tissue is made of 100% new stock, free of
minerals and vegetable filler. This lens tissue does not lint or scratch and will not
disintegrate under vigorous manipulation and is acid-free with excellent wicking properties.