MEB width length diameter measurement

MEB width length diameter measurement

Hi all,

I am very new to imageJ for the moment I'm doing a counting of fiber
diameter manually and I would like to automate it.

The image represents fibers on a filter, I need to measure their
diameter which is actually the apparent width. Also if possible I would
like to know their length but the fiber is not straight, and many fibers
can be crossing.

Re: MEB width length diameter measurement

Hi,

I did pretty much exactly what you are asking recently. I used the
NeuronJ plugin to measure the total length of the fibres and then
generated a binary image to calculate the total area of the image the
fibres occupy. Dividing the area by the length then gave me the mean
width of the fibres in the image.

Dr Colin Rickman
Centre for Integrative Physiology
School of Biomedical Sciences
University of Edinburgh
Hugh Robson Building
George Square
Edinburgh
EH8 9XD

Tel: +44 (0)131 6511512
Fax: +44 (0)131 6503128

Bouvard, Olivia wrote:

> Hi all,
>
>
>
> I am very new to imageJ for the moment I'm doing a counting of fiber
> diameter manually and I would like to automate it.
>
> The image represents fibers on a filter, I need to measure their
> diameter which is actually the apparent width. Also if possible I would
> like to know their length but the fiber is not straight, and many fibers
> can be crossing.
>
> Do you think this is possible using imageJ ? and if yes, how ?
>
>
>
> Regards,
>
>
>
> Olivia
>

--
The University of Edinburgh is a charitable body, registered in
Scotland, with registration number SC005336.

Re: MEB width length diameter measurement

Why not the Distance Map ? Binarize your image, then run the distance
map that will give you the distance to the edges that in your case
correspond to the radius of your fiber. do not forget to adjust contrast
after distance map ;-) .

Thomas

Bouvard, Olivia a écrit :

> Hi all,
>
>
>
> I am very new to imageJ for the moment I'm doing a counting of fiber
> diameter manually and I would like to automate it.
>
> The image represents fibers on a filter, I need to measure their
> diameter which is actually the apparent width. Also if possible I would
> like to know their length but the fiber is not straight, and many fibers
> can be crossing.
>
> Do you think this is possible using imageJ ? and if yes, how ?
>
>
>
> Regards,
>
>
>
> Olivia
>
>

Re: MEB width length diameter measurement

Please see Raub et. al., Biophys. J., (2008), Vol. 94, p. 2361. Two dimensional image autocorrelation will give you a good estimate of the fiber diameters. The length is a much more difficult proposition. In order to autocorrelate in ImageJ, use Process-FFT-FDmath and choose correlate from the middle menu.

I am very new to imageJ for the moment I'm doing a counting of fiber diameter manually and I would like to automate it.

The image represents fibers on a filter, I need to measure their diameter which is actually the apparent width. Also if possible I would like to know their length but the fiber is not straight, and many fibers can be crossing.