Cayman Chemical, in collaboration with BMG
LABTECH GmbH, has developed a novel assay kit to measure mitochondrial function under a dynamic range of oxygen concentrations. The Mito Stress Test (ACU
format) is designed to measure oxygen consumption rate and extracellular acidification simultaneously and in real time under tightly controlled atmospheric
conditions using open-flow respirometry. The assay was designed to work exclusively with microplate readers equipped with an atmospheric control unit and was
optimized using BMG LABTECH’s CLARIOstar microplate reader. By combining these advanced instrumentation and assay technologies, this kit eliminates the
often confounding hyperoxic conditions under which most commercially available, cell-based in-vitro assays are conducted.