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proteins. Page last changed on 19 Dec 2016.

LacZ beta-D-galactosidase

lacZ, beta-Galactosidase

Repressed during biofilm formation. [More information is available at EcoGene: EG10527]. galacotsidase is a metalloenzyme exhibiting broad substrate specificity. [More information is available at EcoCyc: EG10527]. (from
NCBI)

Here we generated fibrin-based mini EHT from a transgenic mouse line (Cdh5-CreERT2 x Rosa26-LacZ) in which EC were specifically and inducible labelled by applying tamoxifen (EC&lt;sup&gt;iLacZ&lt;/sup&gt;). EHT were generated from an unpurified cell mix of new-born mouse hearts and were cultured under standard, serum-containing conditions.

In addition, there are reasons to believe that the biomarkers of aging, proposed by these studies (and in particular, the most popular of them--the activity of senescence-associated beta-galactosidase), are related, as a rule, to the proliferative status of the cells, which in the whole body is generally determined by proper implementing the program of development and differentiation, leading to the emergence of tissues and organs composed of postmitotic or very slowly proliferating cells.

Substrate affinity to N460D-beta-galactosidase was enhanced through greater binding enthalpy (stronger H-bonds through the bridging water) while better affinity to N460T-beta-galactosidase occurred because of greater binding entropy.

study demonstrate an enhancement of beta-galactosidase activity when the enzyme is produced in an oxidizing environment and, consequently, the formation of a disulfide bond in E. coli beta-galactosidase under these conditions

Results on lar, a lac promoter variant indicate the increase as well as the decrease in RNA production with increasing Mg2+ concentration; these changes are, at least partially, caused by changes in the dynamics of formation of the open complex, during transciption initiation.

the hydrophobic and H-bonding properties of Ser-796 result in interactions strong enough to stabilize the open or closed conformations of the loop but weak enough to allow loop movement during the reaction

We demonstrate a variety of applications of stimulated emission microscopy, such as visualizing chromoproteins, non-fluorescent variants of the green fluorescent protein, monitoring lacZ gene expression with a chromogenic reporter, mapping transdermal drug distributions without histological sectioning, and label-free microvascular imaging based on endogenous contrast of haemoglobin.

We found that excessive calorie intake led to the accumulation of oxidative stress in the adipose tissue of mice with type 2 diabetes-like disease and promoted senescence-like changes, such as increased activity of senescence-associated beta-galactosidase, increased expression of p53 and increased production of proinflammatory cytokines.