Dear Ferenc,
I have received several responses concerning the chloroform colony
lysis procedure. Some included a step in which the filter was treated
with lysozyme, DNase and RNase. Have you ever heard of such a thing?
One simple procedure suggested that I lay pipets in a container and pour
Chloroform on the bottom. The filter was then laid on top of the pipets
so that it did not contact the solution. Following treatment with
chloroform, what follows?
Peter