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Specialty Cell Culture Media

ATCC® Number:SCRR-2011™Quantity:
500 mL
Storage:
Store medium at 2°C to 8°C in the dark when not in use.
Applications:
General culture conditions to maintain undifferentiated,
pluripotent mouse ES cells are now well established, and
incremental improvements, such as the development of
Mouse ES Cell Basal Medium, are key to the ongoing
success of stem cell research.

The medium contains a stable glutamine dipeptide, providing
a long shelf-life when handled and stored properly medium may be supplemented by addition of ES-Cell
Qualified Fetal Bovine Serum (FBS) or a serum replacement
designed for mouse ES cells.
This medium can support the growth of mouse ES cells on
either a mitotically inactivated layer of mouse embryonic
fibroblast (MEF) feeder cells, or in a feeder-free environment using gelatin-coated plates.

Using Dermal Cell Basal Medium supplemented with the appropriate growth kit, the growth of keratinocytes and melanocytes are supported without the use of feeder layers, extracellular matrix proteins or other substrates.

Each lot of CellMatrix Gel is shown to promote the attachment of hESCs/hiPSCs and to maintain hESCs/hiPSCs in a pluripotent state as determined by the expression of Oct-4 and Nanog pluripotent markers.

The Adipocyte Differentiation Toolkit for Adipose-derived MSCs and Preadipocytes contains medium and reagents designed to induce adipogenesis in actively proliferating Adipose-Derived Mesenchymal Stem Cells and Preadipocytes with high efficiency, and to support maturation of derived adipocytes during lipid accumulation. The Toolkit is composed of the following components:

One vial containing 1 mL of AD Supplement for use in initiation of adipocyte differentiation

One bottle containing 5 mL of ADM Supplement for use in maintaining adipocyte differentiation

One bottle containing 100 mL of Adipocyte Basal Medium for use in both initiation and maintenance of adipocyte differentiation

*The Adipocyte Differentiation Toolkit for Adipose-derived MSCs and Preadipocytes provides enough medium and reagents for differentiation of ~6.8 x 105 cells when plated at a recommended density of 18,000 viable cells/cm2 in 4 wells of a 6 well tissue culture format.