The present study was to document ethnobotanical information on traditional medicinal plants for the treatment of different ailments by the folk healers of Srivaikuntam Taluk, Tuticorin District, Tamil Nadu. Ethnomedicinal information was gathered from local peoples/vaidyas/housewives/folk healers during the period of November 2017 to March 2018. Forty five medicinal plants belonging to 29 families with their medicinal values were recorded from eight female and three male folk healers. Plant parts used as a medicine includes 50.9% of leaves (28 species), 22.81% of whole plant (12 species), 9.09% of stem bark (5 species) 3.63% of flower, fruit and root (2 species) and 1.81% represented by single species of bulb, seed, stem and rhizome each. Pergularia daemia (Forrsk.) Chiov (Asclepiadaceae), Sida acuta Burm. F. (Malvaceae), Leucas aspera (Willd.) Link (Lamiaceae), Curcuma longa L. (Zingiberaceae), Enicostemma axillare (Lam.) Raynal (Gentianaceae), Phyllanthus amarus Schum. & Thonn. (Euphorbiaceae), Solanum xanthocarpum Schrad. & H. Wendl. (Solanaceae) etc., have been reported to cure cold, dysentery, jaundice, infertility, headache, kidney stone problem, skin diseases, and poisonous bite. The results show that, many of the people from the study area are depend on medicinal plants for primary healthcare.