CRISPR LifePipe®

The first CRISPR application to design both gRNA and donor template sequences required for genome
editing.
CRISPR LifePipe® is a complete and fully customizable solution to facilitate and optimize
your CRISPR experiments.

The introduction of targeted genomic sequence changes by CRISPR technology into living cells has
become a powerful tool for gene therapy or disease modeling. Since CRISPR only requires a nuclease
and customized nucleic sequences, this system can be easily used.

Preliminary bioinformatics analysis for both gRNA and donor templates design can greatly improve
the success of the experiment. This is where CRISPR LifePipe® will make sequence design for genome
editing as simple as using a text editor.

CRISPR: Gene editing is just the beginning. The real power of the biological tool lies in exploring how
genomes work -
Nature, 2016

The guide RNA (gRNA) is a short RNA sequence which guides the Cas9 endonuclease to the targeted region
to cut the genome. gRNA is crucial for CRISPR gene editing because it provides targeting efficiency
on the genomic region of interest while limiting the number of off-targets.

Our gRNA design tool targets different genomic regions: The tool is also configurable with advanced
design options like the GC percent of the sequence or sequence pattern, and off-targets identification
options like maximal number of off-targets per gRNA and mismatches to best meet the user’s needs.

Our tool selects gRNA sequences with the lowest number of off-targets to avoid side effects within
the cell. It also predicts the gRNA efficiency with several annotations like the estimation of
secondary structures, detection of frequent SNPs or computing efficiency prediction scores described
in scientific literature. Finally, a ranking is also provided within the results interface to
help the user choose the best gRNA candidate.

The donor template is a DNA sequence inserted into the cell along with the gRNA and the endonuclease.
It acts by homologous recombination during DNA repair (HDR) to replace the host DNA with the
donor template sequence.

Our tool can design plasmid donor sequences with variable length homology arms as well as ssDNA
oligos (ssODN). Different modes are available to insert modifications into the host:
• Insertion of a mutation into a gene
• Gene tagging in 5’ or 3’

The tool computes the best location on the host genome to insert an exogenous DNA fragment like
a selection cassette to avoid disruption of transcription or splicing of targeted gene.
A donor template quality control is systematically performed to inactivate the gRNA sequence
on the donor template which prevents the endonuclease cutting.

Why choose CRISPR LifePipe®?

Convenient

Our application combines different tools required to perform CRISPR genome editing, from gRNA and
donor template design to the identification of off-targets after NGS

Flexible

All our CRISPR design tools are entirely customizable to consider your specific and evolving needs:
design mode, organism and PAM… A batch mode is also available to help you to parallelize several
designs

Supported

Our application has been developed to include the new findings and ideas published in scientific
literature. CRISPR LifePipe® is supported and regularly updated by our team

Easy to use

Intuitive web form is fully customizable by the user for his specific design needs. An interface
is also available with a
genome browser
to help the user to visualize and download the designs’ results

We have validated CRISPR LifePipe® gRNA design tool by performing a meta-analysis of 10 studies in
human primary cells or cell lines, published between 2016 and 2018.

27 experimentally validated single or paired gRNAs for Cas9sp wt and nickase mutant were extracted
from these publications and studied. All corresponding targets were submitted to CRISPR LifePipe®
gRNA design tool, and the ranking of gRNAs was analyzed.

Firstly, we found that all published gRNAs were found by our application, indicating its high
level of accuracy. Moreover, we observed that most of them ranked in the 3 best gRNAs returned
by our application. All of these results prove the quality of the CRISPR LifePipe® gRNA design
tool and its ability to help scientists to choose the best gRNAs for their experiments.

SFG Congress, 2017

16November , 2017

Life and Soft is a partner of the
2017 congress of the French Society of Genetics, this year focusing on CRISPR. Come and
meet us at the Faculty of Sciences of Montpellier, from November 16th to 18th, to get a glimpse
of the latest advances for our CRISPR design tool: CRISPR LifePipe.

JOBIM Congress, 2017

6July , 2017

Life and Soft was present at the Biology, Bioinformatics and Mathematics Days in Lille. We were
glad to introduce CRISPR LifePipe® in a
poster presentation.

CRISPR LifePipe® Online!

15June , 2017

CRISPR LifePipe, our CRISPR design solution, is now available online. This new version includes
the gRNA and Donor template design functions as well as a new genome browser for result visualization.
Go to
crispr.lifeandsoft.com to get a free trial license.