| '''Homework''': Familiarize yourself with the wiki and particularly the information in the [[BISC110:Resources]] section of the Lab wiki;<br>Lab 1 Practice Problems downloadable here [[Media:Lab_1_Practice_Problems.doc]];<br>

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| '''Homework''': Familiarize yourself with the wiki and particularly the information in the [[BISC110:Resources]] section of the Lab wiki;<br>Lab 1 Practice Problems downloadable here [[Media:Lab_1_Practice_ProblemsS10.doc]];<br>

Read about the process of endocytosis in your textbook and familiarize yourself with phagocytosis in ''Tetrahymena'' from the following reference articles: Gronlien HK, Berg T, Lovlie AM. In the polymorphic ciliate ''Tetrahymena vorax'', the non-selective phagocytosis seen in microstomes changes to a highly selective process in macrostomes. (2002), Journal of Experimental Biology 205, 2089-2097; <BR> McLaughlin NB and Buhse HE, Jr., Localization by indirect immunofluorescence of tetrin, actin, and centrin to the oral apparatus and buccal cavity of the macrostomal form of ''Tetrahymena vorax''., (2004), J Eukaryot. Microbiol., 51(2), 253-257.

Read about the process of endocytosis in your textbook and familiarize yourself with phagocytosis in ''Tetrahymena'' from the following reference articles: Gronlien HK, Berg T, Lovlie AM. In the polymorphic ciliate ''Tetrahymena vorax'', the non-selective phagocytosis seen in microstomes changes to a highly selective process in macrostomes. (2002), Journal of Experimental Biology 205, 2089-2097; <BR> McLaughlin NB and Buhse HE, Jr., Localization by indirect immunofluorescence of tetrin, actin, and centrin to the oral apparatus and buccal cavity of the macrostomal form of ''Tetrahymena vorax''., (2004), J Eukaryot. Microbiol., 51(2), 253-257.

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| Making Dilutions; Determination of toxicity of Heavy Metals on ''Tetrahymena''; Design your own experiment exploring inhibitors and ''Tetrahymena'' phagocytosis

| Making Dilutions; Determination of toxicity of Heavy Metals on ''Tetrahymena''; Design your own experiment exploring inhibitors and ''Tetrahymena'' phagocytosis

| '''Homework''': There are animated tutorials on dilution making available at the following link: [http://www.wellesley.edu/Biology/Concepts/Html/dilutions.html Dilution Help Tutorials]; <br> Finalize your experimental design for Lab 4 and have it approved by your lab instructor;<br> Write a results section of a scientific report on your findings in the ''Tetrahymena'' toxicity experiment including a narrative portion and integrated figures with legends;<br>

| '''Homework''': There are animated tutorials on dilution making available at the following link: [http://www.wellesley.edu/Biology/Concepts/Html/dilutions.html Dilution Help Tutorials]; <br> Finalize your experimental design for Lab 4 and have it approved by your lab instructor;<br> Write a results section of a scientific report on your findings in the ''Tetrahymena'' toxicity experiment including a narrative portion and integrated figures with legends;<br>

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Work the dilution problems found at Media: Lab_3_Dilution_Problems.doc and hand them in in Lab 4.

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Work the dilution problems found at [[Media: Lab_3_S10_Dilution_Problems.doc]] and hand them in in Lab 4.

Draw the expected migration locations of the Fnu4H1 digested PCR product obtained from a homozygous taster (left lane), a homozygous nontaster (center lane) and a heterozygote (right lane) on a diagram of an agarose gel;

Draw the expected migration locations of the Fnu4H1 digested PCR product obtained from a homozygous taster (left lane), a homozygous nontaster (center lane) and a heterozygote (right lane) on a diagram of an agarose gel;

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<br>Answer the questions found at the following downloadable link:[[Media:Taster_DTC_questions.doc‎]] to use as a template for your answers.'''<br><br>

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<br>Answer the questions found at the following downloadable link:[[Media:Taster_DTC_questionsS10.doc‎]] to use as a template for your answers.'''<br><br>

| '''Homework''':Write a scientific paper (all sections except M&M) on your Taster/SNP study in the form of a scientific research report. For help, see the [[BISC110/F09:Resources | Resources]] section of this wiki.

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| '''Homework''':Write a scientific paper (all sections except M&M) on your Taster/SNP study in the form of a scientific research report. '''Due for all students Friday, April 2.'''For help, see the [[BISC110/S10:Resources | Resources]] section of this wiki.

| '''Homework''': Plot your A580 data (y-axis) versus time in seconds (x-axis) for the different treatments with all or some of the treatments on the same graph.([[Appendix E]]). Determine regression equations for the linear part of each data plots to get slope (m) values (slopes represent the rates of electron transport). Calculate the rates (change over time of the linear portion of your graphs) and plot those values against your variable (x-axis);<br>

| '''Homework''': Plot your A580 data (y-axis) versus time in seconds (x-axis) for the different treatments with all or some of the treatments on the same graph.([[Appendix E]]). Determine regression equations for the linear part of each data plots to get slope (m) values (slopes represent the rates of electron transport). Calculate the rates (change over time of the linear portion of your graphs) and plot those values against your variable (x-axis);<br>

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A full paper on your experiment today in the form of a scientific research report is due at beginning of Lab 11. Discuss your results in terms of how the factor that you chose to investigate affects the electron transport rate in photosynthesis. Compare your results to other investigations published in the primary literature. Refer to the "[[BISC110:Guidelines for writing a lab report in the form of a scientific paper | '''Guidelines for science writing''']]" information found in the [[BISC110/F09:Resources | Resources]] section of the wiki.

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A full paper on your experiment today in the form of a scientific research report is due at beginning of Lab 11. Discuss your results in terms of how the factor that you chose to investigate affects the electron transport rate in photosynthesis. Compare your results to other investigations published in the primary literature. Refer to the "[[BISC110:Guidelines for writing a lab report in the form of a scientific paper | '''Guidelines for science writing''']]" information found in the [[BISC110/S10:Resources | Resources]] section of the wiki.

Read about the process of endocytosis in your textbook and familiarize yourself with phagocytosis in Tetrahymena from the following reference articles: Gronlien HK, Berg T, Lovlie AM. In the polymorphic ciliate Tetrahymena vorax, the non-selective phagocytosis seen in microstomes changes to a highly selective process in macrostomes. (2002), Journal of Experimental Biology 205, 2089-2097; McLaughlin NB and Buhse HE, Jr., Localization by indirect immunofluorescence of tetrin, actin, and centrin to the oral apparatus and buccal cavity of the macrostomal form of Tetrahymena vorax., (2004), J Eukaryot. Microbiol., 51(2), 253-257.

Homework: Study dilution making for Lab 3; Generate two figures with legends from your Tetrahymena experiment: one or more microphotographs of Tetrahymena and a graph or table of data.

1

3

Tues. Feb. 9- Tues. Feb. 16

Making Dilutions; Determination of toxicity of Heavy Metals on Tetrahymena; Design your own experiment exploring inhibitors and Tetrahymena phagocytosis

Homework: There are animated tutorials on dilution making available at the following link: Dilution Help Tutorials; Finalize your experimental design for Lab 4 and have it approved by your lab instructor; Write a results section of a scientific report on your findings in the Tetrahymena toxicity experiment including a narrative portion and integrated figures with legends;

Homework:Write a scientific paper (all sections except M&M) on your Taster/SNP study in the form of a scientific research report. Due for all students Friday, April 2.For help, see the Resources section of this wiki.

3

8

Mon. Mar. 29- Fri. Apr. 2

Photosynthesis 1: Photosynthetic pigments

Homework: Prepare a full results section for the photosynthetic pigment identification experiment;

Homework: Finalize your self-designed experiment testing a variable affecting the Hill Reaction; Write a Materials and Methods style version of the Hill Reaction experiment that you did this week in Lab 9

Homework: Plot your A580 data (y-axis) versus time in seconds (x-axis) for the different treatments with all or some of the treatments on the same graph.(Appendix E). Determine regression equations for the linear part of each data plots to get slope (m) values (slopes represent the rates of electron transport). Calculate the rates (change over time of the linear portion of your graphs) and plot those values against your variable (x-axis);

A full paper on your experiment today in the form of a scientific research report is due at beginning of Lab 11. Discuss your results in terms of how the factor that you chose to investigate affects the electron transport rate in photosynthesis. Compare your results to other investigations published in the primary literature. Refer to the "Guidelines for science writing" information found in the Resources section of the wiki.

4

11

Tues. Apr. 20- Thurs. Apr. 29

Enzymology

Homework: Prepare for a Lab Practical Skills Assessment in lab 12.

Answer the following questions (5 points) and hand them in to your instructor at the beginning of Lab 12. Download a Word document form of these questions here:Media: Lab_11_Questions_110.doc Make sure Lab Notebook is ready to turn in for grading