A practical procedure for determining estrogens in biological fluids has been studied using liquid chromatographyâ€“electron capture atmospheric pressure chemical ionizationâ€“mass spectrometry combined with derivatization. Among the commercially available reagents (4-nitrobenzoyl chloride, 2,4-dinitrofluorobenzene, 4-nitrobenzenesulfonyl chloride and 4-nitrobenzyl bromide), 4-nitrobenzenesulfonyl chloride was of the most practical use; it rapidly and quantitatively reacted with estrogens and increased the detection responses by 8â€“23 times. The derivatization method allowed the reproducible and accurate quantification of serum and urine estrone and estradiol of a pregnant woman, which is useful for diagnosis of the fetoplacental function, with small amounts (10 Î¼l) of sample and a simple pretreatment procedure. [Figure not available: see fulltext.]

ACCESSION #

22458637

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