Intracellular Ca2+ signaling imagine

The dynamic modulation of Ca2+ concentration in ReproNeuro™ differentiated neuronal cells can be observed with the application of a fluorescent calcium binding dye (Fluo-8). Glutamine derived induction of calcium flux was shown in nearly all of the applied neurons.

Before induction

After 100 μM Glutamine induction

Courtesy of Prof. Oka and Mr. Enya, Keio University

Dopamine release by dopaminergic neurons derived from ReproNeuro™

ReproNeuro™ cells derived from human iPS cells were first treated with high concentration Ca2+ (56 μM) to polarize the cells. After polarization, increased dopamine concentration in the culture medium was detected, indicating the functional capability of dopamine release in ReproNeuro™ cells.

Transgenic Alzheimer's disease model cells

Custom disease model cells on your request

We provide services making custom disease model cells on customer's request. This service includes acquisition of a patient sample, establishment of human iPS cells from the patient sample by RNA based reprogramming, and differentiation into functional cells (Cardiomyocytes, Hepatocytes and Neurons). Please contact us for details.

Data Sheet

Safety Data Sheets

References

Publications

Odawara, Aoi, Masao Gotoh, and Ikuro Suzuki. “A three-dimensional neuronal culture technique that controls the direction of neurite elongation and the position of soma to mimic the layered structure of the brain.” RSC Adv. (2013).