Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10166-LR

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Desired Reconstitution Concentration

Background: LAIR2

LAIR2
(leukocyte-associated Ig-like receptor-2; CD306) is a secreted, 131 amino acid
(aa) protein that contains one Ig-like C2 type domain, making it a member of
the Ig superfamily. When compared to LAIR1, its transmembrane counterpart,
LAIR2 shares 83% aa identity across the signal sequence and extracellular
domains (1-3). Although one is secreted and the other is membrane-bound, the
two LAIR proteins are thought to have arisen from a common gene ancestor and
appear to share similar adhesion profiles. This suggests that LAIR2 may
compete with LAIR1 for ligand binding (3, 4). A 114 aa alternate
splice form of LAIR-2 is truncated at the C-terminus, but retains the entire Ig
domain (1-3). The expression profile of these splice forms, and the presence of
orthologs in other species, have not been reported. LAIR2 is a soluble
collagen-receptor, and it can be detected in the synovial fluid of rheumatoid
arthritis patients, urine of pregnant women, and as well as primary cells (5, 6).
In vitro studies have demonstrated LAIR2
can compete with LAIR1 for the same collagen binding site and suggesting
LAIR2 may play an important role in immune cell activation (5, 6). LAIR2 can
interact with complement component 1q (C1q) and mannose-binding lectin (MBL) and
act as a complement inhibitor for the treatment and prevention of
antibody-mediated allograft rejection and antibody-mediated clinical conditions
(7).