nuc-seq/SNES

A modified MDA protocol, nuc-seq takes advantage of the fact that a single cell in the G2_M stage of the cell cycle has 4 copies of the genome. This property allows the cells to be isolated with a cell sorter, and it also significantly increases the genome coverage of single cells (Wang et al., 2014). SNES is an additional variation that includes targeted selection and sequencing of the exomes (Leung et al., 2015). Div-Seq is a variation that combines nuc-seq with pulse labeling of proliferating cells by 5-ethynyl-2_-deoxyuridine (EdU) Habib et al., 2016)

Advantages:

nuc-seq: Improves physical coverage performance to more than 90% for single-cell sequencing

SNES: 95.94% exome coverage in single cells

SNES: Detection efficiencies of 92.37% for SNVs in an isogenic population

Edit: nuc-seq/SNES

History: nuc-seq/SNES

A modified MDA protocol, nuc-seq takes advantage of the fact that a single cell in the G2_M stage of the cell cycle has 4 copies of the genome. This property allows the cells to be isolated with a cell sorter, and it also significantly increases the genome coverage of single cells (Wang et al., 2014). SNES is an additional variation that includes targeted selection and sequencing of the exomes (Leung et al., 2015). Div-Seq is a variation that combines nuc-seq with pulse labeling of proliferating cells by 5-ethynyl-2_-deoxyuridine (EdU) Habib et al., 2016)

Advantages:

nuc-seq: Improves physical coverage performance to more than 90% for single-cell sequencing

SNES: 95.94% exome coverage in single cells

SNES: Detection efficiencies of 92.37% for SNVs in an isogenic population

A modified MDA protocol, nuc-seq takes advantage of the fact that a single cell in the G2_M stage of the cell cycle has 4 copies of the genome. This property allows the cells to be isolated with a cell sorter, and it also significantly increases the genome coverage of single cells (Wang et al., 2014). SNES is an additional variation that includes targeted selection and sequencing of the exomes (Leung et al., 2015). Div-Seq is a variation that combines nuc-seq with pulse labeling of proliferating cells by 5-ethynyl-2_-deoxyuridine (EdU) Habib et al., 2016)

Advantages:

nuc-seq: Improves physical coverage performance to more than 90% for single-cell sequencing

SNES: 95.94% exome coverage in single cells

SNES: Detection efficiencies of 92.37% for SNVs in an isogenic population

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