Current revision

Objective

Take out the hydrogels to thaw, crosslink the quaternary ammonium salt films made monday (0.01%), make 1% QAS film, filter the 10% QAS, and look up concentrations for the next hydrogel reaction.

Description

The hydrogels were removed from the freezer at 10 am and were put back in at 2 pm.

The quaternary ammonium salt films made on monday could not be crosslinked because they had not set.

The 10% QAS film was filtered using a vacuum pump and a buchner funnel.

Quaternary Ammonium Salt Film Preparation

Measure out two 1 g samples of the 1% QAS

Place in beaker with 30 mL water. Dissolve on heat.

Once dissolved, add 1 mL glutaraldehyde. Allow to stir for 3 minutes.

Pour into plastic dish and allow to evaporate until Friday.

Next Hydrogel Reaction (Click Chemistry)

PNAS article A chemical method for fast and sensitive detection of DNA synthesis in vivo by Adrian Salic and Timothy J. Mitchison was used to determine the ranges of concentrations for the reactants for the click reaction.