Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

normal

Age

100 days

Gender

male

Strain

C3H/An

Storage Conditions

liquid nitrogen vapor phase

Karyotype

modal number = 52; range = 46 to 54; This is a hyperdiploid cell line with 24 to 26 marker chromosomes common to all cells., Nine markers (M1 - M9) are common to L-M(TK-) (see ATCC CCL-1.3) and 16 to 17 are found only in A9. The latter markers include Rb(3.5), Rb(4.15), Rb(5.5), T(8;19), Rb(9.17) and 11 to 12 others., Structurally normal chromosomes 4, 15, 16, 17, 18 and X were absent. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.

Derivation

The A9 cell line was isolated by J.W. Littlefield from wild-type Strain L cells.

Clinical Data

male

Antigen Expression

H-2k

Tumorigenic

Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10exp7 cells.

Effects

Yes, in nude mice

Comments

The cells are sensitive to HAT selection medium and deficient in adenosine phosphoribosyl transferase (APRT) and hypoxanthine phosphoribosyl transferase (HPRT).

The cells are resistant to 8-azaguanine (0.003 mg/mL) and 6-thioguanine (0.1 mM).

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.