[ssml] error from BLASTCLUST

Hello,
Thanks for the reply to my BLAST query.
This time I have another query about BLASTCLUST which I am trying to use to
clsuter my dataset. In the documentation it says by default it uses BLOSUM62
with gap penalities etc.
Now I want to use default options so I just simply give my dataset as input
file & give output file names.
Problem is it throws warning & error saying
"[NULL_Caption] WARNING: SetUpBlastSearch failed.
[NULL_Caption] ERROR: BLASTSetUpSearch: Unable to calculate Karlin-Altschul para
ms, check query sequence"
it means it didn't find lamda & K values in precomputed tables so giving
warning & errors. normally it should be there anyway I can provide that values,
the question is HOW? in BLASTCLUST there is no option for providing these
values.
Could you help me out, what to do in this case. & why it is giving error?
regards,
Manoj
Quoting Kevin Karplus <karplus at soe.ucsc.edu>:
> The matrix is not the whole set of parameterization for BLAST.
> There are also the gap costs and the lambda and K values used for
> computing E-values.
>> Changing the matrix without correcting the other parameters leads to
> uninterpretable results.
>> Kevin Karplus karplus at soe.ucsc.eduhttp://www.soe.ucsc.edu/~karplus> Senior member, IEEE Board of Directors, ISCB (starting Jan 2005)
> Professor of Biomolecular Engineering, University of California, Santa Cruz
> Undergraduate and Graduate Director, Bioinformatics
> Affiliations for identification only.
>
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Manoj TYAGI
Laboratoire de Biochimie et Génétique Moléculaire
Université de La Réunion
BP 7151, 15 avenue René Cassin
97715 Saint Denis Messag Cedex 09
La Réunion
FRANCE
Tel : +262 262 938641
Fax : +262 262 938237
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