It was assessed in this study, the effects of glyphosate on the growth of two coffee species distinct growth. We used a factorial (2 x 5) in a randomized block design with four replications, with treatments consisting of two coffee species (Coffea arabica L.): Catucaí Yellow (2 SL) with short internodes and Acaiá (IAC 474/19) with long internodes, and five doses of glyphosate (0, 57.6, 115.2, 230.4 and 460.8 g ha-1). The herbicide was applied when the coffee plants had 44 and 52 cm high, and referring to Catucaí Acaiá, respectively. On the application of glyphosate and also at 45 and 120 days after treatment, evaluated the increases in height, leaf area, number of primary branches and leaves and stem diameter. In the variables assigned the value of 100% plants that received no treatment with glyphosate. From this value we calculated the dose effects on plants, percentages above or below 100% (reference value) with respect to growth of between 45 and 120 days after herbicide application. We conclude that the Acaiá is less tolerant to glyphosate when compared to Catucaí, therefore, this cultivar showed lower growth than Catucaí when treated with herbicide.