In order to verify the role of fiber bragg grating strain gauge in strain measurement of concrete bridge, and the influence of concrete and steel strain properties by fatigue loading, the relevant experimental researches were carried out. The results indicated that the Fiber Bragg grating strain gauge could reflect the strain regularity of concrete and steel well; for the fiber grating strain rosettes inside the concrete, the pasting equipments of fiber gratings had a certain impact of the measurement effect. The fatigue loading had a certain impact of the properties of concrete and steel, in conditions of the same number of fatigue, the greater the stress amplitude the more obvious the effects. The results analysis shown that, fiber grating strain measurement innovated the means and methods of strain measurement in detailed structure of the concrete bridge, for the strain measurements of concrete bridge under fatigue loading, the FBG could still play very good effect.