A spider inventory from forest floor and understory in 2 sites of the cloud montane forest of the Biosphere Reserve Volcán Tacaná, Chiapas was undertaken. Soil spiders were collected using pitfall traps and processing litter in Berlese funnels; understory spiders were captured by hand collecting, sweeping, beating, and with shelter traps. Collected specimens correspond to 32 families, 99 genera and 151 species, which represent 32.5% of the spiders recorded for the state of Chiapas. There are 7 new records of genera and 12 of species for Mexico and 6 genera and 9 species for Chiapas. The families with most species richness were Theridiidae, Linyphiidae, Anyphaenidae, Araneidae and Salticidae. In the forest floor were recorded 75 species, 57 genera and 26 families and in the understory 112 species, 74 genera and 22 families. Both strata shared 36 species, and 39 and 76 species were exclusive to the forest floor and to the understory respectively. Complementarity between the 2 strata was 76.2%.