BlastR Rapid Lysate Prep Kit

More Views

The BlastR™ rapid lysate prep kit allows for fast, reliable, and quantitative preparation of genomic DNA (gDNA)-free cell lysate for western blot or immunoprecipitation. The benefit of this prep kit is a combination of the unique denaturing BlastR™ buffer combined with the proprietary BlastR™ filters. The denaturing BlastR™ buffer allows for complete protein isolation from all cellular compartments, but its composition allows for easy protein quantiation with conventional protein assays unlike most denaturing buffers such as Laemmli. Additionally, the BlastR™ filter effectively removes gDNA contamination which is a significant problem with denaturing buffers that can affect downstream applications like immunoprecipitation and migration in SDS-PAGE. Simply put, the BlastR™ rapid lysate prep kit provides all the superior benefits of using a denaturing buffer system with the ease of performing a standard non-denaturing lysis buffer preparation. The BlastR™ rapid lysate prep kit is an integral component of our Signal-Seeker™ products and is efficient for studying protein modifications for any target protein.

(B) Sample passed through Filter system where gDNA is captured. >90% recovery of protein in cell lysate.

Comparison of BlastR™ versus commonly used non-denaturing and denaturing lysis buffers. Isolation of proteins from the membrane, cytoplasm, mitochondria, and nucleus.

A431 cells were lysed with BlastR™, RIPA, mPER, IP lysis, Denaturing (1% SDS), and Laemmli lysis buffers. Isolation of proteins from the membrane, cytoplasmic, mitochondrial, and nuclear markers were determined using the respective compartment marker proteins. The western blot figure highlights the ability of BlastR™ buffer to isolate proteins from all cellular compartments similarly to other denaturing buffers. Unlike ofther denaturing buffers, the BlastR™ buffer is compatible with standard protein assays, which is critical when equivalent protein loading is essential. BlastR™ buffer is also an excellent IP buffer when used with the BlastR™ dilution buffer as outlined in the datasheet. These features are outlined in the table below for easy comparison with available lysis buffers.