Amnis® ImageStream®XMk II

High resolution microscopy and flow cytometry to advance your discovery

Amnis® ImageStream®X Mk II Imaging Flow Cytometer

The revolutionary ImageStream®X Mk II Imaging Flow Cytometer combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. This unique combination enables a broad range of applications that would be impossible using either technique alone.

The ImageStreamX Mk II System is a benchtop, multispectral, imaging flow cytometer designed for the acquisition of up to 12 channels of cellular imagery. By collecting large numbers of digital images per sample and providing a numerical representation of image-based features, the ImageStreamX Mk II combines the per cell information content provided by standard microscopy with the statistical significance afforded by large sample sizes common to standard flow cytometry. With the ImageStreamX Mk II System, fluorescence intensity measurements are acquired as with a conventional flow cytometer; however, the best applications for the ImageStreamX Mk II take advantage of the system’s imaging abilities to locate and quantitate the distribution of signals on in or between cells.

Featured ImageStreamXMk II Video

Watch how imaging in flow has changed research at Roswell Park Cancer Institute (RPCI).

At the RPCI Flow and Image Cytometry facility, Drs. Paul Wallace, Hans Minderman, and staff provide users with the best tools available for increasing statistical power and throughput for applications like measuring NFkB translocation that were formerly performed by laborious microscopy. Watch to learn how Amnis protocols have helped RPCI researchers detect rare events like circulating tumor cells.

Features

Fast: Analyzes Thousands of Cells Per Second

Easy-to-Use: Simple User interface

The intuitive user interface provides real-time plotting and graphical gating, as well as images of every cell. The easy to use compensation wizard quickly guides you through setup of multi-color compensation matrices.

Flexible: Fits Your Sample, Your Targets, Your Fluorophores

The ImageStreamX II Imaging Flow Cytometer accepts up to 7 lasers and works with sample volumes of 20-200 μL for added experimental flexibility — perfect for multi-user laboratories.

Efficient: Less Sample Volume, Less Sample Waste

Fluidics that provide up to 95% sample utilization when every cell is required. Recover unused sample for further analysis when orthogonal methods are required.

Adaptable: Any Upgrade, Any Time

The modular design of the ImageStreamX Mk II Imaging Flow Cytometer allows the system to grow with your research needs and budget through a broad range of field-installable options. Most options, including additional lasers, can be installed at the factory or upgraded in the field by Luminex-certified field service personnel.

Imaging Flow Cytometers – Research Areas

The powerful combination of quantitative image analysis and flow cytometry in a single platform creates exceptional new experimental capabilities. The following are examples of areas where the ImageStreamX Mk II and FlowSight Imaging Flow Cytometers can bring more power and insights into your research.

Immunology

Oncology

Biochemistry

Drug Discovery

Stem Cell Biology

Hematology

Microbiology

Virology

Nanotechnology

Toxicology

Oceanography

Parasitology

Amnis ImageStreamX Mk II Imaging Flow Cytometer Options

Laser Options

An Expandable Fluorescent Palette

The standard 488 nm laser of the ImageStreamX Mk II Imaging Flow Cytometer may be augmented with up to six additional lasers, including 375, 405, 561, 592, and 642 nm. A high-power 488 nm laser upgrade is also available for enhanced detection of fluorescent dyes excited at that wavelength.

Lasers and Example Dyes

AutoSampler

AutoSampler for Multiwell Plates

The AutoSampler option for the ImageStreamX Mk II Imaging Flow Cytometer enhances productivity with unattended sample loading from multiwell plates. The fully integrated AutoSampler option allows you to easily perform dose response and time course studies even in primary samples, due to the system’s capacity for imaging large numbers of cells from each sample.

Features

Ideal for high throughput analysis of suspended cells

Easy to use acquisition templates allow for multiple experiments per plate

12 Image Channels

Up to 12 Images of Every Cell

The 12 Image Channel option significantly expands the experimental flexibility and analytical potential of the ImageStreamX Mk II Imaging Flow Cytometer by doubling the number of images per cell from six to twelve. This option is comprised of a second image detection system, including a filter wheel, inline filters, spectral decomposition assembly, and a proprietary charge-coupled device (CCD) camera.

Improve throughput via multiplexing of experiments in a single tube or sample well

Field installable by certified technicians

Application: Nuclear Translocation of NFκB

The analysis of NFκB nuclear translocation shown below used three lasers and eight of 12 available detection channels. Cells were phenotyped using fluorescently-labeled antibodies against CD14, CD45, CD16, and CD3, while two additional image channels were used to quantitatively assess nuclear localization of NFκB by cross-correlation of the nuclear (DAPI) and NFκB (FITC) images. Brightfield and darkfield (SSC) images of each cell are included to provide morphological and structural detail.

MultiMag

60X/40X/20X Magnification

The MultiMag option provides 20X and 60X objective lenses, in addition to the standard 40X objective, for greater experimental flexibility and improved resolution. The 60X objective lens increases magnification for small objects, such as yeast and bacteria, and offers greater detail with mammalian and plant cells. The 20X objective lens provides an increased field of view for the imaging of large cells, such as epithelial cells.

The Right Magnification for Your Work

60X magnification for higher resolution

20X magnification for large cells

Extended Depth of Field

A unique optical system upgrade for the ImageStreamX Mk II Cytometer keeps the depth of cell in focus without loss of fluorescence sensitivity. Now you can image FISH spots or other sub-cellular features, no matter where they lie in the cell.

Image more of the cell in focus

The extended depth of field (EDF™) option for the ImageStreamX Mk II Cytometer incorporates Wavefront Coding™ technology from CDM Optics, which is a combination of specialized optics and unique image processing algorithms, to project more structures within the cell into one crisp plane of focus.

EDF technology dramatically extends the depth of field, enabling new applications and improving the performance of existing applications – especially spot counting applications such as FISH, autophagy, and nuclear translocation.