Bovine Tensin-4 (TNS4) ELISA Kit

Product Information

Product name

Bovine Tensin-4 (TNS4) ELISA Kit

Reactivity

Bovine

Applications

ELISA

Application notes

This Bovine Tensin-4 (TNS4) ELISA Kit employs a two-site sandwich ELISA to quantitate TNS4 in samples. An antibody specific for TNS4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNS4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNS4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNS4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection method

Colorimetric

Sample type

Cell culture supernatants, Other biological fluids, Plasma, Serum

Assay type

Sandwich ELISA (quantitative)

Assay duration

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Fig.1. Bovine Tensin-4 (TNS4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Bovine Tensin-4 (TNS4) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine TNS4. No significant cross-reactivity or interference between Bovine TNS4 and analogues was observed.

Calibration range

56.25-3000 pg/mL

Limit of detection

60.2 pg/mL

Usage notes

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage instructions

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping

Gel pack with blue ice.

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background

Tensin-4 (TNS4) may be involved in cell migration, cartilage development and in linking signal transduction pathways to the cytoskeleton and may promote apoptosis, via its cleavage by caspase-3.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.