Application Information: AXIN1 antibody, C-term

Preliminary experiments gave bands at approx 150kDa and 22-24kDa in Human Brain (Hippocampus) lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 95.6kDa according to NP_003493.1 and 91.7kDa according to NP_851393.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? In paraffin embedded Human Adrenal Gland shows membranous and fibrous staniing in and around secretory cells of the zona fasciculata in the cortex. Recommended concentration, 3-5µg/ml. This antibody is expected to recognise both reported isoforms.

Positive Controls

The peptide used to product this antibody is available for purchase (Catalog Number:GTX89836-PEP).

Form Supplied

Liquid

Concentration

0.5
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Specifications: AXIN1 antibody, C-term

Full Name

axin 1

Background

Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development.