- best match to Acidobacteria bacterium Ellin345 (35% identity) - matches in Bacteria, not A and not E - also CO0318 IQ lipid transport and metabolism - no matches on other clones - length good start GTG

- envirnoment conserved in Ellin345 and Ellin6706 and cloneAD370_D1_152? - and in addition ribosomal protein L32 - DUF177, Rib L12, fatty acid synthase in syntheny in Acidobacteria and also in other Bacteria: Geobacter, Pelobacter....

LUAcid_AD370_D1_07

13703

13882

ribosomal protein L32

1

D

ADDED

- best match Acid345_4144 (60% id) - very conserved in B - Pfam1783

PROTEIN

-

B

82.227.62.180

14/09/07 17:57:22

- envirnoment conserved in Ellin345 and Ellin6706 and cloneAD370_D1_152? - and in addition ribosomal protein L32 - DUF177, Rib L12, fatty acid synthase in syntheny in Acidobacteria and also in other Bacteria: Geobacter, Pelobacter....

- environment conserved in Ellin345 and Ellin6706 and cloneAD370_D1_152? - and in addition ribosomal protein L32 - DUF177, Rib L12, fatty acid synthase in syntheny in Acidobacteria and also in other Bacteria: Geobacter, Pelobacter....

- best match to Planctomyces maris DSM 8797 (52% id) - very high number of paralogs in each genome with orthologs (Planctomycetes 64 hits) - only present in a small number of Bacteria...Poribacteria...

PROTEIN

Good

B

82.227.62.180

14/09/07 22:20:17

- strong similarities to KM3_18_F8_163 and KM3_18_F8_128 - several paralogs (10+) in Solibacter, not in Elllin345 - often in tandem with DUF1549 protein as on clone KM3_18_F8 ORF128 and 116 - here not in tandem - ortholog on Poribacteria fragment

LUAcid_AD370_D1_27

32796

34130

glutamate-1-semialdehyde-2,1-aminomutase

1

D

ADDED

5.4.3.8

- best match to Lentisphaera araneosa (47%) - very conserved protein in bacteria and archaea - also COG4992 and COG0160

PROTEIN

COG0001 Glutamate-1-semialdehyde aminotransferase

H Coenzyme transport and metabolism

Good

AB

82.227.62.180

14/09/07 22:29:17

- good match on the two genomes - ortholog to KM3_18_F8_249

LUAcid_AD370_D1_28

34222

35229

N-acetyl-gamma-glutamyl-phosphate reductase

1

D

ADDED

1.2.1.38

- best match to Solibacter usitatus (62% id) - very conserved in bacteria but also in Archaea

PROTEIN

COG0002 Acetylglutamate semialdehyde dehydrogenase

E Amino acid transport and metabolism

Good

AB

82.227.62.180

14/09/07 22:36:41

- one ortholog on the two genomes - not on clones

LUAcid_AD370_D1_29

35263

36039

acetylglutamate kinase

1

D

ADDED

2.7.2.8

- best match to Solibacter usitatus (51% id) - often in tandem with glutamate-1-semialdehyde-2,1-aminomutase as here with 346

PROTEIN

COG0548 Acetylglutamate kinase

E Amino acid transport and metabolism

Good

AB

82.227.62.180

14/09/07 22:45:23

- one match on the two genomes

LUAcid_AD370_D1_30

36292

36603

putative regulatory protein, FmdB family

1

D

ADDED

- best match to Solibacter usitatus (55% id)

PROTEIN

COG2331 Uncharacterized protein conserved in bacteria

S Function unknown

Good

B

82.227.62.180

14/09/07 22:54:56

- one good match in the two genomes - lower match in KM3_205_D9_425

LUAcid_AD370_D1_31

37575

38174

threonyl-tRNA synthetase (truncated c-terminal)

1

D

ADDED

- best match to Acidobacteria bacterium (54% id)

PROTEIN

COG0441 Threonyl-tRNA synthetase

J Translation, ribosomal structure and biogenesis

Good

82.227.62.180

14/09/07 22:58:57

- one match in the two genomes

LUACID_AD370_D1_01

1595

372

ABC efflux pump, inner membrane subunit, DUF214

1

R

ADDED

- best match to Acidobacteria bacterium Ellin345 (35% identity) - matches in Bacteria, not A and not E - also CO0318 IQ lipid transport and metabolism - no matches on other clones - length good start GTG