One of the greatest difficulties for investigators working with various primary lung and heart cell populations is obtaining consistently viable, highly purified, cell sub-populations for ex vivo analyses from cardiopulmonary sources in an efficient and reproducible manner. And while each investigator in this COBRE has a varying degree of expertise in such cell isolations, a centralized resource for the isolation, propagation, and characterization of cells from the heart and lung are fundamentally important services for all Investigators, and foundational to the success of the CPVB COBRE.