Type

Applications

Sample Requirements

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

31.3–2000 pg/ml

Limit of Detection

15.5 pg/ml

Intra-assay (Within-Run)

CV = 9.5%

Inter-assay (Run-to-Run)

CV = 5.8%

Summary

Research topic

Cytokines and chemokines and related molecules

Summary

Interleukins 17F (IL-17F) and 17A (IL-17A) are closely related members of the IL-17 cytokine family, sharing about 50% amino acid identity. Studies in the mouse have identified Th17 cells as a distinct CD4+ T cell lineage that is defined by the production of IL-17F and IL-17A. IL-6 and transforming growth factor-β are required for the differentiation of naïve CD4+ T cells to Th17 cells, which are maintained in the presence of IL-23 and IL-1β. Conversely, IL-4 and interferon-γ can inhibit the development of Th17 cells. Th17 cells have been implicated
in the pathology of mouse autoimmune disease models. Expression of IL-17F and IL-17A has been detected in activated Human peripheral blood lymphocytes. It has been shown that the cytokines are expressed in activated Human CD4+ T cells. Expression of IL-17F and IL-17A has also been observed in tissue samples from various
autoimmune diseases such as rheumatoid arthritis, multiple sclerosis, psoriasis, inflammatory
bowel disease, and asthma.
The crystal structure of IL-17F has been solved and shows that the protein forms a disulfidelinked dimeric glycoprotein. IL-17A is also a disulfide-linked homodimeric glycoprotein. The IL- 17F homodimer includes a classical cysteine knot motif, which is found in the TGF-β, bone morphogenetic protein, and nerve growth factor superfamilies. One difference in the cysteine knot motif of IL-17F compared with the other known cysteine knot protein families is that it only utilizes four cysteines instead of the classical six cysteines to form the knot. IL-17F and IL-17A have been shown to form biologically active IL-17F/IL-17A heterodimers, in addition to the IL- 17F and IL-17A homodimers.