Development of an integrated chip for automatic tracking and positioning manipulation for single cell lysis.

Young CW, Hsieh JL, Ay C - Sensors (Basel) (2012)

Bottom Line:
The average speed of cell driving was 17.74 μm/s.This technique will be developed for DNA extraction in biomolecular detection.It can simplify pre-treatment procedures for biotechnological analysis of samples.

ABSTRACTThis study adopted a microelectromechanical fabrication process to design a chip integrated with electroosmotic flow and dielectrophoresis force for single cell lysis. Human histiocytic lymphoma U937 cells were driven rapidly by electroosmotic flow and precisely moved to a specific area for cell lysis. By varying the frequency of AC power, 15 V AC at 1 MHz of frequency configuration achieved 100% cell lysing at the specific area. The integrated chip could successfully manipulate single cells to a specific position and lysis. The overall successful rate of cell tracking, positioning, and cell lysis is 80%. The average speed of cell driving was 17.74 μm/s. This technique will be developed for DNA extraction in biomolecular detection. It can simplify pre-treatment procedures for biotechnological analysis of samples.

Mentions:
After cell lysis, the fluorescent dye YO-PRO-1 was added to the lysis absorbing cell solution, which was then observed under a microscope. Through a 100× immersion lens and B-cell nucleus radiation, the DNA image of the post-lysis U937 cell could be observed in Figure 11. This proves that the trans-membrane formed by the combination of the tip microelectrode and AC in this system architecture can succeed in cell lysis for intracellular DNA extraction.

Mentions:
After cell lysis, the fluorescent dye YO-PRO-1 was added to the lysis absorbing cell solution, which was then observed under a microscope. Through a 100× immersion lens and B-cell nucleus radiation, the DNA image of the post-lysis U937 cell could be observed in Figure 11. This proves that the trans-membrane formed by the combination of the tip microelectrode and AC in this system architecture can succeed in cell lysis for intracellular DNA extraction.

Bottom Line:
The average speed of cell driving was 17.74 μm/s.This technique will be developed for DNA extraction in biomolecular detection.It can simplify pre-treatment procedures for biotechnological analysis of samples.

ABSTRACTThis study adopted a microelectromechanical fabrication process to design a chip integrated with electroosmotic flow and dielectrophoresis force for single cell lysis. Human histiocytic lymphoma U937 cells were driven rapidly by electroosmotic flow and precisely moved to a specific area for cell lysis. By varying the frequency of AC power, 15 V AC at 1 MHz of frequency configuration achieved 100% cell lysing at the specific area. The integrated chip could successfully manipulate single cells to a specific position and lysis. The overall successful rate of cell tracking, positioning, and cell lysis is 80%. The average speed of cell driving was 17.74 μm/s. This technique will be developed for DNA extraction in biomolecular detection. It can simplify pre-treatment procedures for biotechnological analysis of samples.