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Tri Your Best

Yin-Yang breaking the first science rule.

It was discovered by Yin-Yang, after their repetitive presses on the "Water" and "Dr. Fizz" buttons in the Soda Machine, located on the ground level. After pressing them interleaved for 10 times, a tube appeared outside of the Soda Machine, sucking them in.

Hmmm...

There, it was revealed to be Test Tube's secret laboratory, the place where Test Tube create her inventions and experiments, including the Bow-in-nator, and Experiment 626, a liquid that helps separate Yin-Yang from each other. After the separation, Yang jumped out of the lab, leaving a hole in the lab's roof

The lab appears once again in this 1 million views anniversary video. In the lab, there are now new devices, the View Counter. It is used to counts the views on all episodes, if not just Tri Your Best and Breaking The Ice. Apparently, if one counter reaches 1 million, its capacity will be closer to the maximum count and will starts to malfunction. Viewing the distressing scene was everyone that was competing in Season 2, other than Tissues, Box, and Yin-Yang[1].

The view counter reaches a million.

When the first counter, for the episode Tri Your Best reaches a million, everyone in the lab started to panic. While Fan and Paintbrush was discussing for an idea to stop the viewers from watching their videos before another counter reaches a million. Shortly after, the second counter, for Breaking The Ice, reaches that amount of views. The scene suddenly changes to the Soda Machine on the ground level, with a muffled explosion sound. It is unknown if the lab actually exploded, or the video and the scene itself was non-canon.

During a discussion with Taco, Microphone spontaneously comes across Test Tube's lab, which results in her crossing out the "secret" in the sign, so the lab is no longer a secret. There is also a new unknown gadget in the lab that allows time travel and dimension hopping. Due to its resemblance to a calculator, Lightbulb attempts to determine how much money she owes Test Tube, and accidentally sends the two to the very first episode of Inanimate Insanity.

Trivia

It is unknown how everyone got into the laboratory in the Inanimate Insanity II T-Shirt Anniversary video, however, it is suspected that Test Tube let them in to view the scene of the View Counter reaching a million.