TNFRII is a member of the TNFR family of transmembrane proteins, and is expressed in immune cells and certain endothelial cells. It is a high affinity receptor for TNF-α but manifests a lower affinity to TNF-β. Signaling through this receptor regulates various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. Soluble TNFRII is capable of inhibiting TNF-α induced activities by acting as a decoy receptor.

Source:

E.coli.

Purity:

> 98% by SDS-PAGE and HPLC analyses.

Endotoxin Level:

< 0.1 ng per ug of sTNF-receptor.

Stabilizer:

None.

Formulation:

Lyophilized.

Biological Activity:

Determined by its inhibitory effect of the TNF-α mediated cytotoxicity in murine L-929 cells. The ED50for this effect in the presence of 0.25 ng/ml of recombinant human TNF-α, is 0.125 μg/ml.

Reconstitution:

We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20℃ for future use.

Stability:

The lyophilized protein is stable for at least 2 years from date of receipt at -20℃. Reconstituted sTNFRII is stable for at least 3 months when stored in working aliquots with a carrier protein at -20℃. Avoid repeated freeze-thaw cycles.