Vit C

In article <4eii8q$9fg at decaxp.harvard.edu>, robison at lipid.harvard.edu
(Keith Robison) wrote:
>IAN DWORKIN (idworkin at ivory.trentu.ca) wrote:
>: In article <31004A7C.6EDC1F90 at mackiller.llnl.gov>, Chris Barry
<chbarry at mackiller.llnl.gov> writes:
>: >I read on one of the newsgroups recently that man is one of the few
>: >mamals that don't produce vitamine C. There were a couple of follow up
>: >articles to the thread that seemed to confirm the statement. The
>: >statement has been bothering me since. I seem to recall that some people
>: >can produce vitamine C. Am I wrong? Are you aware of an article that can
>: >confirm/refute their statement? Thanks in advance.
>: >
>>: >Chris
>>: As far as I know, no human is capable of producing ascorbic acid, the enzyme
>: which we are missing for the completion of the biosynthetic [pathway is
present
>: in the human genome as a pseudogene. Since it intiailly lost its function, it
>: has become a neutral marker, and thus has been subject to alot of nucleotide
>: substitutions. If i remember correctly there are a few exons which are
>: reasonably intact, but I would have to look it up. If you are very
interested
>Could you post the name of the enzyme? This sort of question
>comes up periodically.
>>Keith
I'm not an expert but here is some the header for the GenBank entry that I
dug up from a Medline search:
LOCUS HUMGULOB 3364 bp DNA PRI 15-SEP-1995
DEFINITION Human GULO gene for L-gulono-gamma-lactone oxidase, exon 9,10 and
12.
ACCESSION D17461
KEYWORDS L-gulono-gamma-lactone oxidase; biosynthesis of L-ascorbic acid;
flavin enzyme.
SOURCE Homo sapiens peripheral blood (library: lambda phage, EMBL4) DNA,
clone lambda HGO8.
ORGANISM Homo sapiens
Eukaryotae; mitochondrial eukaryotes; Metazoa; Chordata;
Vertebrata; Sarcopterygii; Mammalia; Eutheria; Primates;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3364)
AUTHORS Nishikimi,M., Fukuyama,R., Minoshima,S., Shimizu,N. and Yagi,K.
TITLE Cloning and chromosomal mapping of the human nonfunctional gene for
L-gulono-gamma-lactone oxidase, the enzyme for L-ascorbic acid
biosynthesis missing in man
JOURNAL J. Biol. Chem. 269 (18), 13685-13688 (1994)
MEDLINE 94230484
_____
Jerry Learn
Research Associate
Dept. of Microbiology | Learn at u.washington.edu
University of Washington | Phone: (206) 616-4286
Box 357740 | FAX: (206) 616-1575
Seattle, WA 98195-7740 USA | or (206) 543-8297