origami bacteria transformation

Hello. I am trying to transform Origami B (DE3) host cells but I find lots of problems to grow and transform the cells. I used cacl2 to make them competent.The cells didn't grow on the selection plate after transformation. (Using the same protocol, BL21 cells have been transformed efficiently).

Thanks for your answers. I used standard CaCl2/heat shock method for preparation and transformation of competent cells. After transformation, approximately 100 ul of transformed competent cells were transferred onto agar lB medium and incubated at 37°C.No colony was appeared after 24 hours.(origami cells grow slowly)

I'd suggest that rather than guessing, you should measure the competence of your cells. Likely it is very poor, with this protocol. I'd suggest doing it with BL21 (DE3) cells in parallel, so that you can compare with cells you know work. You measure competence by transforming with serial dilutions of a known working plasmid, finally determining colony forming units per microgram of plasmid. For BL21 strains, something in the 10^5 CFU/ug is a reasonable number. If necessary, you can try a higher efficiency competent cell protocol.

Are you paying attention to the antibiotic resistances of these strains? Some of the unusual BL21 derivatives have plasmids already with chloramphenicol resistance (Rosetta) or chrlomosomal integrations carrying resistances kanamycin and tetracycline (Origami 2 (DE3). So, if you were trying to transform a plasmid with Kan resistance, all the cells would grow, and you perhaps would see the lawn and think that no colonies formed. Always check the genotype. This is easy to spot -- streak a plate rather than spread it.