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HMW : The IRDye 680LT dye bears an NHS ester reactive group that will couple to free amino groups and form a stable conjugate with high molecular weight proteins (45 - 200 kDa).

LMW : The IRDye 680LT dye bears an NHS ester reactive group that will couple to free amino groups and form a stable conjugate with low molecular weight proteins (15 - 45 kDa).

Microscale : The IRDye 680LT dye bears an NHS ester reactive group that will couple to free amino groups and form a stable conjugate with 100 µg of protein with molecular weight 14 - 200 kDa. Fluorescent conjugates labeled with IRDye 680LT display an absorption maximum of 680 nm and an emission maximum of 694 nm in 1X PBS.