This is the first study to document that the viral E1 and E2 proteins were necessary and sufficient to support replication of a papillomavirus origin in a transient transfection assay.

Ustav et al.,
1991. See above.

This study correctly identified and mapped the bovine papillomavirus origin of replication. This was the first authentic definition of a functional papillomavirus origin, and documented that a DpnI assay could be used to successfully detect transient replication of papillomavirus origin–containing DNA.