Villains from any type of comedy can be put here, even if the villain themselves is not especially funny; even pure evils and game changers can be put here. Examples include Dag from Barnyard,Katz from Courage the Cowardly Dog, and Thrax from Osmosis Jones who are serious and evil characters, but come from comedy settings.

A comic relief usually can never be in this category unless there is a game changer or a pure evil in that story, considering the comic reliefs are supposed to lighten up a very serious story, and comedies are rarely serious stories.