tbzmedTabriz University of Medical SciencesPHARMResearch ArticleDetermination
of Some Cephalosporins in Pharmaceutical Formulations by a Simple and Sensitive
Spectrofluorimetric MethodSpectrofluorimetric Determination of Some CephalosporinsAbdollahiAli , Bavili-TabriziAhaduniversity of medical sciences, Medicinal Chemistry32016303201622112122015Tabriz University of Medical Sciences2015Determination
of Some Cephalosporins in Pharmaceutical Formulations by a Simple and Sensitive
Spectrofluorimetric Method

Background: Cephalosporins are among the safest and the most
effective broad-spectrum bactericidal antimicrobial agents which have been
prescribed by the clinician as antibiotics. Thus, the developing of simple, sensitive and rapid
analytical methods for their determination can be attractive and desirable.
Methods: A
simple, rapid and sensitive spectrofluorimetric method was developed for the
determination of cefixime, cefalexin and ceftriaxone in pharmaceutical
formulations. Proposed method is based on the oxidation of these cephalosporins
with cerium (IV) to produce cerium (III), and its fluorescence was monitored at
356 &plusmn; 3 nm after excitation at 254 &plusmn; 3 nm.
Results: The
variables effecting oxidation of each cephalosporin with cerum (IV) were
studied and optimized. Under the experimental conditions used, the calibration
graphs were linear over the range 0.1-4 &micro;g/mL. The limit of detection and limit
of quantification were in the range 0.031-0.054 and 0.102-0.172 &micro;g/mL,
respectively. Intra- and inter-day assay precisions, expressed as the relative
standard deviation (RSD), were lower than 5.6 and 6.8%, respectively.
Conclusion: The
proposed method was applied to the determination of studied cephalosporins in
pharmaceutical formulations by good recoveries in the range 91-110%.