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7 A SUMMARY OF THE THOUGHT PROCESS It is our hope that this chapter has given the reader some guidelines for interpreting how a particular mutation might affect the structure and function of a protein. These include upregulation at the RNA level of vascular endothelial growth factor, in particular isoform 189 (45,46), which is highly relevant in terms of neovascularization.

Pipet100μLofcontentsofeachethanolstandardintoaquartzcuvetteandread absorbance at 340 nm (see Note 10). The αi and αo types of subunit are myristoylated, which explains their ability to remain associated with the membrane after release from the β γ dimer.

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