I have just recently started using a guanidine thiocyanate extraction method
for extracting viral RNA. I want to know how long the extraction solution
can be stored for and under what conditions (i.e. frozen, at 4 C, etc.).
Any information from other people using this method would be greatly
appreciated. The extraction solution contains 8M guanidine isothiocyanate,
50mM Na citrate, 100 mM 2-ME, 1% sarkosyl, 1 ug/ml yeast tRNA.
Ta.
Loryn Sellner
Department of Microbiology