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Special blocking procedure/product for using mouse antibody on mouse tissue

Special blocking procedure/product for using mouse antibody on mouse tissue - Western Blot Forum

Special blocking procedure/product for using mouse antibody on mouse tissue - Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

Re: Special blocking procedure/product for using mouse antibody on mouse tissue

Hi there,

You have to use anti - mouse secondary antibody if there is mouse - primary antibody. Are you talking about your secondary antibody? I think you need an anti-mouse antibody, it's not possible to have mouse anti-mouse antibody so it would be an anti-mouse antibody raised in a different species, e.g. goat anti-mouse, rabbit anti-mouse, ect.

Re: Special blocking procedure/product for using mouse antibody on mouse tissue

I have a mouse anti-human antibody against a gene which is conserved between the two species. I am attempting to use the antibody against the mouse gene however I am also getting considerable background in my westerns. I've tried blocking using an unconjugated Fab fragment for mouse IgG however it doesn't seem to have eliminated all the background.