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The Ghostbusters discovered the presence of Necrotic Slime after blasting two Zombies. Realizing it turned things into Zombies, the team strips off their slime drenched uniforms as a precaution. Egon Spengler rigged a Zombie radar to detect the entities based on some Necrotic Slime he saved. The slime could be neutralized when a zombie is possessed by a new type of poltergeist. The true purpose was to use the Zombies' Necrotic Slime to infect Gozer's essence in a bid to steal a fraction of it. [4] Britt decided to leave some Necrotic Slime in Newark, New Jersey.

The slime turns living organisms it touches into zombies through a process known as "hastened ectoplasmic migration from a corporeal form." That is, the soul leaves the body. For an adult male, it took about two days for this process. For a stray cat, it was nearly instantaneous. Egon is able to readjust the P.K.E. Meter to detect the slime.