The female hamster has two clearly seen openings, the vagina and the anus. The vagina is just above the anus, and their is very little space between them. In adult animals, this area is surrounded by pigment. These two openings are positioned very close together.

The male hamster also has two openings, the penis and the anus. By the time the hamsters are weaned, the distance between these two openings should be clearly seen in male hamsters. Also as it comes closer to the time of weaning, the male hamster's testicles will begin to drop, giving the rear end a rounded appearance compared to that of the female.