The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27kD monomer consisting of 238aa and emits green light (emission maximum at 509nm) when excited with blue or UV light.

Catalog #

G8965-07A

Applications

Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Dot Blot. Other applications not tested.

Recommended Dilution

Western Blot: 1:1,000

Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Clone Type

Monoclonal

Isotype

IgG1

Clone No

10K226

Host

Mouse

Concentration

~1mg/ml

Form

Supplied as a liquid in 10mM PBS, PH 7.2, 0.1% sodium azide.

Purity

Purified.

Immunogen

GFP from the jellyfish Aequorea Victoria N-terminal peptide (KLH).

Specificity

Recognizes native and denatured forms of GFP and its variants EGFP, YFP, EYFP, and CFP.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.