Application Information: Carbonic Anhydrase IX antibody

IHC-P: Use at a dilution of 1/1000. Immunocytochemistry/Immunofluorescence 1/1000. IHC-Fr 1/1000. WB 1:2000. Optimal dilutions/concentrations should be determined by the end user.

Positive Controls

Renal carcinoma

Form Supplied

Liquid

Concentration

1.1
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Immunogen affinity purified

Specifications: Carbonic Anhydrase IX antibody

Full Name

carbonic anhydrase IX

Product Description

Rabbit polyclonal to Carbonic Anhydrase IX

Background

Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. Because of its functionality, it has become an important diagnostic marker for various cancers, most notably renal cell carcinoma (RCC). There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and location within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.