[Histonet] Transfecting GFP gene into cells in suspension

From:

"hamdi abuali"

Hello All
Currently I am trying to transfect umbilical cord cells with GFP gene , the cells are not cultures, they are in suspension , and I do not want to culture them becuase I am studying their maturity level and I dont want any factors to promote differentiation .
I would like to know if anyone has a protocol in that regard .
Thank you
Hamdi Abu-Ali, MD.
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Hamdi Abu-Ali , MD.
University of South Florida
Cardiology - Tampa, Florida
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