Custom antibody development

We offer world-leading antibody development platforms based on a proprietary RabMAb® rabbit monoclonal platform, a phage display platform (AxioMx), and a next-generation sequencing platform (NGS-RabMAb®)

Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

ELISA

Application notes

ELISA: Use at an assay dependent dilution.
Immunoblotting: Use at an assay dependent dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Low-density lipoprotein (LDL) is the carrier protein for cholesterol in the blood. LDL binds to its receptor on the capillary walls and thereby mediates the uptake and clearence of cholesterol from the circulation. In atherosclerotic lesions oxidatively modified LDL is found and oxidized LDL is specifically recognized and ingested by macrophages via scavenger receptor A and CD36. Oxidized LDL may be a marker of atherosclerosis but the precise changes in oxidized LDL are not well described. MDA-oxidized LDL appear to be different from LDL oxidized by other means.

Cellular localization

Secreted

Alternative names

Low density lipoprotein antibody

MDA oxidized LDL antibody

MDA oxidized low density lipoprotein antibody

References

ab17354
has not yet been referenced specifically in any publications.

Publishing research using ab17354? Please let us know so that we can cite the reference in this datasheet.

Thank you for getting back to me and explaining these issues to me.
I am convinced that there is a problem with this antibody in the system that you are using. I would like to offer you a credit note provided the antibody was purchased within the pa...

Thank you for your enquiry.
I have been in touch with the source of this antibody and they have provided me with the following information.
"I am afraid that only MDA (malon di aldehyde) oxidized LDL will work, as mentioned in the product speci...

Thank you for your enquiry.
Yes, this antibody reacts with oxidized LDL but not with native LDL.
I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Abcam Scientific Support

Answered on Jan 19 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"