Bottom Line:
However, little is known regarding the effect of microRNA (miRNA) on GLA's anti-metastatic activity.Here, we found that GLA attenuated the migratory and invasive capacity of breast cancer cells by activating miR-200c.Overexpression of miR-200c enhanced the expression of E-cadherin and decreased the expression of vimentin.

Mentions:
Glabridin (GLA) did not appreciably affect the vitality of MDA-MB-231 or BT-549 breast cancer cells at concentrations of 0.0, 5.0, 10.0 or 20.0 μM (Fig. 1a). To determine the effects of GLA on the motility of breast cancer cells, wound healing assays were performed. MDA-MB-231 and BT-549 cells displayed high motility, but GLA reduced such capability in a dose-dependent manner (Fig. 1b). Because 10.0 μM GLA effectively decreased the motility of these cells, this concentration was selected for further investigations. As determined with transwell assays, GLA decreased both the migratory and invasive capacities of MDA-MB-231 and BT-549 cells (Fig. 2a–c).

Mentions:
Glabridin (GLA) did not appreciably affect the vitality of MDA-MB-231 or BT-549 breast cancer cells at concentrations of 0.0, 5.0, 10.0 or 20.0 μM (Fig. 1a). To determine the effects of GLA on the motility of breast cancer cells, wound healing assays were performed. MDA-MB-231 and BT-549 cells displayed high motility, but GLA reduced such capability in a dose-dependent manner (Fig. 1b). Because 10.0 μM GLA effectively decreased the motility of these cells, this concentration was selected for further investigations. As determined with transwell assays, GLA decreased both the migratory and invasive capacities of MDA-MB-231 and BT-549 cells (Fig. 2a–c).

Bottom Line:
However, little is known regarding the effect of microRNA (miRNA) on GLA's anti-metastatic activity.Here, we found that GLA attenuated the migratory and invasive capacity of breast cancer cells by activating miR-200c.Overexpression of miR-200c enhanced the expression of E-cadherin and decreased the expression of vimentin.