After
incorporation of uracil into the DNA strand, UDG catalyses the
hydrolysis of the n-glycosidic bond between uracil and deoxyribose.
Before starting the PCR an incubation at 37 °C for 10 minutes degrades
uracil containing DNA fragments from previous experiments. The enzyme
is deactivated by heat shock at 95 °C for 10 minutes.