Abstract: Molecularly imprinted polymeric membranes with the tripeptide residue H-Glu(OBzl)-Phe-Phe-CH2- (EFF) were prepared during the membrane preparation (casting) process in the presence of a print molecule. The Ac-L-Trp imprinted polymeric membranes thus obtained showed adsorption selectivity toward Ac-L-Trp. From adsorption isotherms of Ac-Trp isomers it is seen that the chiral recognition site formed in the presence of the print molecule in the membrane preparation process exclusively recognizes the isomer Ac-L-Trp which was the print molecule, while the opposite isomer, Ac-D-Trp, is rigorously rejected. The affinity constant between Ac-L-Trp and the chiral recognition site was determined to be 9.6 x 10(3) mol(-1) dm(3). Enantioselective permeation can be attained by applying electrodialysis as a driving force. An optimum separation factor toward the L-isomer of 6.8, which corresponds to the adsorption selectivity, can be reached