Membrane chromatography (MC) has been increasingly used these last years and has become a well-established technique of preparative chromatography. The aim of this state of the art review is to present some of the recent developments of this purification technique. First, the main stationary
phases used for preparative chromatography are recalled, including resin beds, membranes and monoliths. The second part of the review is devoted to the interaction modes which can be used in MC, such as ion exchange, affinity and hydrophobic interactions. In the next section, advances made
on device geometries are summarized including flat sheets, hollow fibers, spiral and crossflow devices. The main advances in MC modelling are then presented with models including dispersive effects in the experimental set-ups and recent computational fluid dynamics (CFD) models. In the last
section, several applications of MC are summarized: proteins, monoclonal antibodies and virus purification, as well as other emerging applications like lactoferrin isolation from whey.