SpecificityThis assay has high sensitivity and excellent specificity for detection of AKAP4. No significant cross-reactivity or interference between AKAP4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AKAP4 and all the analogues, therefore, cross reaction may still exist in some cases.

Product Description specificalIntended Uses: This AKAP4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative `determination of Human AKAP4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: AKAP4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AKAP4 antibody and an AKAP4-HRP conjugate. The assay sample and buffer are incubated together with AKAP4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AKAP4 concentration since AKAP4 from samples and AKAP4-HRP conjugate compete for the anti-AKAP4 antibody binding site. Since the number of sites is limited, as more sites are occupied by AKAP4 from the sample, fewer sites are left to bind AKAP4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AKAP4 concentration in each sample is interpolated from this standard curve.