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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is
committed to providing our customers around the world with the highest quality products.
In addition to direct selling in more than 20 countries in North America, Europe and
Asia/Pacific, Miltenyi Biotec also provides support for our customers through an
extensive distributor network covering dozens of additional countries.

As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is
committed to providing our customers around the world with the highest quality products.
In addition to direct selling in more than 20 countries in North America, Europe and
Asia/Pacific, Miltenyi Biotec also provides support for our customers through an
extensive distributor network covering dozens of additional countries.

How can I isolate human platelets by depletion of unwanted cells?

Below you find a customer protocol for the isolation of platelets by depletion of unwanted cells:

Start with 9.0 mL of citrate-stabilized blood.

Centrifuge for 10 min at 200xg at room temperature. This yields a thrombocyte-rich supernatant with 3x107 erythrocytes, 1.4x106 lymphocytes, and 5x108 platelets.

Wash three times with 500 µL PBS containing 2 mM citrate and 0.5% BSA. The flow-through will contain the untouched platelets. If a higher concentration of platelets is required, use only one washing step.