The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternate splicing of this gene results in many isoforms that localize to the centrosome and the Golgi apparatus, and interact with numerous signaling proteins from multiple signal transduction pathways. These signaling proteins include type II protein kinase A, serine/threonine kinase protein kinase N, protein phosphatase 1, protein phosphatase 2a, protein kinase C-epsilon and phosphodiesterase 4D3.

Catalog #

A1102-83A

Applications

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution

Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Clone Type

Polyclonal

Isotype

IgG

Host

Goat

Source

Human

Concentration

~0.5mg/ml

Form

Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.

Purity

Purified by immunoaffinity chromatography.

Immunogen

Peptide with sequence CSGSTTQFHAGMR, from the C Terminus of the protein sequence according to NP_005742; NP_671695; NP_671700; NP_671714.

Specificity

Important Comments: This antibody is expected to recognize isoforms 1, 2 and 3 (NP_671700.1, NP_005742.4, NP_671714.1, respectively). The isoforms Yotiao, AKAP350B and AKAP350C are not recognized.

Species Crossreactivity

Expected from sequence similarity: Human, Mouse

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.