Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Applications

Approved:

Flow Cytometry

Specificity and Use

Immunogen:

FcERI / Fc Epsilon RI antibody was raised against human FCER1A

Usage:

The AER-37 (CRA1) antibody has been pre-titrated and tested by flow cytometric analysis of peripheral blood leukocytes. This can be used at 20 ul (0.125 ug)/per test. A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

Presentation:

Aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary

Storage:

Store at +4°C. Do not freeze. Product is photosensitive and should be protected from light.

Description: Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.