Facility Personnel

Roman Krawetz, PhD

Facility Director

Dr. Roman Krawetz is an assistant professor in the Departments of Surgery/Cell Biology & Anatomy whose research is focused on understanding the mechanisms regulating the interaction between inflammation and stem cell mediated cartilage repair and develop novel diagnostic & prognostic assessments of OA.

Laurie Kennedy

Facility Manager

Laurie’s research background is in B cell development. She was the principle operator/manager of the first BD FACSCan purchased by the Immunology Research Group and have been providing training for post graduate students since 1994. She joined the Core Facility in 2005 and is the principal instructor for the USER Facility

Yiping Liu

Facility Staff

Yiping obtained her MD in China in 1988, and PhD in Cell Biology in 1997. She gained extensive Flow Cytometry experience as lab manager of the BD ARIA in Jan Storek’s lab. She joined the Core Facility in 2010. Yiping has extensive experience with flow cytometry multicolor design and trouble shooting.

INSTRUMENTATION

ABI Attune

The Attune Acoustic Focusing Flow Cytometer is the first cytometer that uses ultrasonic waves, rather than hydrodynamic forces to position cells into a single, focused line along the central axis fof the capillary.

Features and Specification Highlights

24/7

trained users can access the facility 24/7

2

two lasers; blue/red or blue/violet

6

up to 6 colours for multiplexed analysis

Software

free ABI software for offline analysis

BD LSRII

BD LSRII is the first and only air-cooled, four-laser bench top flow cytometer with the ability to acquire up to 18 colours. At the heart of the BD LSRII is the new digital acquisition, allowing for additional parameters to be detected and yielding more information per cell.

LSR II Features and Specification Highlights

4

equipped with 4 lasers on both LSRII

16

Up to 16 colours for multiplexed analysis

full

full service acquisition and analysis

2

2 LSRIIs for quick turnaround

Cell Sorters

BD AriaIII

The fluidics system in the BD FACSAria™ III cell sorter is pressure driven. Positive air pressure forces sample cells through an optically gel-coupled cuvette flow cell. Hydrodynamic focusing guides particles in a single-file stream through the cuvette, where laser light intercepts the stream at the sample interrogation point.

Features and Specification Highlights

4

equipped with 4 lasers on both Aria’s

flexible

sample loading from plate or tube format

16

up to 16 colours for multiplexed sorting

2/4

2 or 4 way sorting into tubes/plates

Sony SH800 Z

The benchtop SH800S cell sorter permits sorting of a wide range of cell sizes and applications using the 70 μm, 100 μm, and 130 μm microfluidics sorting chips. This novel, chip-based design is fully integrated with comprehensive fluidics controls and advanced automation for set-up, acquisition, sort and analysis to make sorting less subjective, more precise and easier to use.

System software is intuitive and supports sorting into tubes and 96- and 384-well plates. The software generates FCS 3.0 and FCS 3.1 files that also can be exported to third party analysis tools.