Classifications

C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans

C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates

C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals

C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used

C07K14/4722—G-proteins

C—CHEMISTRY; METALLURGY

C07—ORGANIC CHEMISTRY

C07K—PEPTIDES

C07K2319/00—Fusion polypeptide

Abstract

The invention provides a series of Gαq rotein variants that functionally couple to sensory cell receptors such as taste GPCRs(Trs) and olfactory GPCRs(ORs) in an overly promiscuous manner. According to the invention, the functional coupling can be determined, for example, by measuring changes in inracellular IP3, or calcium. In a particular embodiment, the Gαq protein variants can be expressed in mammalian cell lines or Xenopus oocytes, and then evaluated using calcium fluorescence imaging and electriphysiological recording.

PCT/US2001/0326192000-10-302001-10-24GαqPROETIN VARIANTS AND THEIR USE IN THE ANALYSIS AND DISCOVERY OF AGONISTS AND ANTAGONISTS OF CHEMOSENSORY RECEPTORS
WO2002036622A3
(en)