Re: Antigen Retrieval

From:

Tom Wells <tomers@home.com> (by way of Histonet)

Tammaryn,
We have been using a bench top autoclave for our antigen retrieval for
several years. At first we only used it for select antigens now we use it
for everything that requires antigen retrieval. We set the timer for 25
minutes and the temperature for 240. In addition we use Dako's target
retrieval solution. The only problem that we have had is that if you bring
the autoclave down to room temperature too quickly the lids may pop off the
coplin jars. We eliminated that problem by using different containers which
don't have the screw top lid. I hope this helps. Tom
Tom Wells, BSc, ART
Supervisor, Immunohistochemistry Laboratory
Lions Gate Hospital
North Vancouver, BC
Canada
> > Date: 8 Dec 2000 11:16:56 -0600
> > From: Tammaryn Lashley <t.lashley@ion.ucl.ac.uk>
> > Subject: Antigen retreival
> >
> > Dear All,
> >
> > Does anybody out there have any experience with using an autoclave as a
> > means of antigen retrieval in fixed tissue?
> >
> > Tammaryn
> > Dep. of Neuropathology
> > Institute of Neurology
> > Queens Square House
> > Queens Square
> > London WC1N 3BG
> >
> > Tel: 0171 837 3611 ext 4247
> >
> >
> >