*Using TBE allows us to have such a high (150V) voltage. If you use TAE you need to use a significantly lower voltage and your run time will be longer. Despite that, TAE is advantagous in some cases, but [[User:Michael_A. Speer | Mike]] feels that TBE is better suited for his applications. This is discussed in detail in the [[agarose gel electrophoresis | consensus protocol]].

*Using TBE allows us to have such a high (150V) voltage. If you use TAE you need to use a significantly lower voltage and your run time will be longer. Despite that, TAE is advantagous in some cases, but [[User:Michael_A. Speer | Mike]] feels that TBE is better suited for his applications. This is discussed in detail in the [[agarose gel electrophoresis | consensus protocol]].

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* Use the following table to determine the amount of agarose you want to use.

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* Use the following table to determine the amount of agarose you want to use.<br><br>

Notes

Using TBE allows us to have such a high (150V) voltage. If you use TAE you need to use a significantly lower voltage and your run time will be longer. Despite that, TAE is advantagous in some cases, but Mike feels that TBE is better suited for his applications. This is discussed in detail in the consensus protocol.

Use the following table to determine the amount of agarose you want to use.