2.
[2] found high proportions engaging in HIV risk behaviors.
The National HIV/AIDS Strategy for the United States [3]
identiﬁes injecting drug users (IDU) as a priority popula-
tion for HIV prevention efforts. However, the illicit, stig-
matized nature of injection drug use makes surveillance
and sampling of IDU for research difﬁcult. IDU are a
hidden population which cannot be accessed using standard
sampling methodologies.
Respondent-driven sampling (RDS) is a peer-referral
sampling and analysis method that provides a way to
account for several sources of bias and calculate population
estimates [4–6] now widely used to reach hidden popula-
tions including many at high risk for HIV [7]. IDU are an
especially well-suited population for RDS methodology
because they rely on social networks for much of their
livelihood, including access to drugs, income, and safety.
This reliance requires them to form cohesive social
exchange networks, which are ideal for RDS. Furthermore,
IDU are difﬁcult for researchers to study due to the stig-
matized, illegal nature of their activities and the danger
posed to ﬁeld researchers working in their communities [8].
RDS’ peer-to-peer recruitment of respondents fosters trust
and promotes participation while also reducing ﬁeld staff’s
exposure to dangerous environments while conducting
research [9].
RDS has become increasingly popular in studies of IDU
in the U.S. [10, 11] and abroad [7]. While studies collecting
RDS data are numerous, many RDS analytical techniques
are still under development due to the complex nature of
RDS data and relatively short time since its debut in 1997
[12]. One currently under-developed technique is a priori
sample size calculation.
Calculating sample size requirements is a necessary
preliminary step to proposing, planning, and implementing
a successful study. However, in RDS, this calculation is
complicated by the peer-driven nature of RDS data and
their analysis [13]. RDS analysis is similar to analysis of
stratiﬁed samples where sampling weights are applied
during analysis to adjust for non-uniform sampling prob-
ability. However, while such samples are usually stratiﬁed
by variables of interest, such as race or income, with preset
selection probabilities determined by the researcher, RDS
samples are stratiﬁed by the target population’s underlying
network structure which is unknown to researchers. For
this reason, data on network structure are collected during
sampling and used to calculate sampling weights post hoc
[4]. Thus, sample size estimation, which requires knowing
selection probabilities, cannot currently be directly calcu-
lated a priori.
Cornﬁeld [14] proposed that sample size for complex
surveys can be calculated by ﬁrst calculating the sample
size required for a simple random sample (SRS) and then
adjusted by a measure of the complex sampling method’s
efﬁciency compared to SRS, termed the design effect (DE)
[15]:
n ¼ DE Á
Pa 1 À Pað Þ
SE Pað Þð Þ2
ð1Þ
where n is the sample size; Pa 1ÀPað Þ
SE Pað Þð Þ
2 is the common for-
mula for calculating required SRS sample size for some
proportion Pa; SE is the standard error; and DE is the
design effect of the actual sampling method used.
Following Kish [15] we deﬁne DE as the ratio of the
variance of the estimate observed with RDS, VarRDS
ðPaÞ,
to the expected variance of the estimate had the sample
been collected using simple random sampling (SRS),
VarSRS
ðPaÞ as follows:
DE ¼
VarRDS
ðPaÞ
VarSRS
ðPaÞ
ð2Þ
where VarSRS
ðPaÞ ¼ Pað1ÀPaÞ
n : Consequently, DE compares
the observed variance under a complex sampling method—
in this case RDS—to that expected for the same estimate
under an SRS of similar size [15]. Consequently, DE
measures the increase in sample size required to achieve the
same power as that of an SRS. For example, a sampling
design with DE = 3 requires a sample three times as large
as an SRS to achieve the same power [16]. Comparing
sampling methods to SRS is mathematically convenient
here because calculating power, and consequently estimat-
ing sample size, is straightforward for SRS. Consequently,
knowing DE for a given sampling method provides a means
for estimating sample size [14]. However, SRS and RDS are
not directly comparable in an empirical setting. RDS was
developed to reach hidden-populations which, by deﬁnition,
cannot be sampled using SRS methods. A comparison of
RDS to other hidden-population methodologies, such as
time-location sampling, is beyond the scope of this paper;
however we expect all such methods to have similar limi-
tations in comparison to SRS [17].
The deﬁnition of DE assumes knowledge of variance
associated with a given estimate. However, the only way to
truly measure the variance would be to take repeated
samples from the same population simultaneously. Given
limited resources, researchers are left with two options for
estimating the underlying variance: (1) estimate the vari-
ance based on a single sample of real world data or (2)
create an estimate of the population and simulate repeated
samples from that estimate. In both approaches, the
researcher is forced to make mathematical assumptions
regarding the behavior of participants and the probability
of selection. For established sampling methods, such as
SRS, this is not problematic because the probabilities of
selection are well understood and a single, agreed upon
variance calculation exists for real (not simulated) data. For
798 AIDS Behav (2012) 16:797–806
123

3.
less established methods, such as RDS, the sampling
probabilities are not yet well understood and multiple
methods of calculating variance may exist with no agreed
upon best approach. In such cases, the calculated DE rep-
resents an estimated design effect, denoted cDE throughout
this paper.
To date, few studies of RDS cDEs have been conducted.
One often-cited publication suggests RDS samples have a
cDE of at least two and recommends samples sizes should be
at least double that required for a comparable SRS design
[18]. However, An RDS study of sex workers in Brazil
reported cDE = 2.63 [19]. Another RDS study of under-
graduate students found an average cDE of 3.14 [13]. Fur-
thermore, using simulated data, Goel and Salganik [20] ﬁnd
RDS cDEs may reach above 20, suggestingRDS analysis may
produce highly unstable samples. While empirical evidence
suggests RDS estimates are too accurate to support such
large cDEs [21], further research is needed to determine if a
generalized cDE can be applied to RDS studies of certain
populations or if the commonly applied recommendation of
cDE = 2 should be adjusted. If, for example, a review of
many RDS studies found relatively consistent cDEs across
variables and samples, then this cDE could be applied to
calculate sample size in future RDS studies.
Methods and Data
Methods for NHBS among IDU (NHBS-IDU) are described
in detail elsewhere [10] and brieﬂy reported here. NHBS is a
community-based survey that conducts interviews and HIV
testing among 3 high risk populations: IDU, men who have
sex with men, and heterosexuals at increased risk for HIV
infection [22]. Data used in this paper were collected during
the ﬁrst two cycles of NHBS-IDU (NHBS-IDU1 from 2005
to 2006 and NHBS-IDU2 in 2009). NHBS-IDU is con-
ducted by the Centers for Disease Control and Prevention
(CDC) in collaboration with state and local health depart-
ments in over 20 of 96 large metropolitan statistical areas
(those with population greater than 500,000) within the
United States (termed ‘‘cities’’ throughout), where approx-
imately 60% of the nation’s AIDS cases had been reported
[23]. Health departments in each city received local IRB
approval for study activities. CDC reviewed the protocol
and determined CDC staff was not-engaged; therefore CDC
IRB approval was not required.
Each city operated at least one interview ﬁeld site that
was chosen to be accessible to drug-use networks identiﬁed
during formative research. Following standard RDS pro-
cedures [12, 24], each city began RDS with a limited
number of diverse initial recruits, or ‘‘seeds’’ (n = 3–35).
Respondents were provided number-coded coupons with
which to recruit other IDU they knew personally. The
number of coupons given to each respondent varied by city
and ranged from three to six. Within some cities, the
number of coupons given varied throughout sampling to
regulate the ﬂow of individuals seeking interviews and to
reduce the number of coupons in the community as the
sample size was approached. Respondents were compen-
sated both for their participation and for each eligible
recruit who completed the survey.
NHBS-IDU procedures included eligibility screening,
informed consent from participants, and an interviewer-
administered survey. Eligibility for NHBS-IDU included
being age 18 or older, a resident of the city, not having
previously participated in the current NHBS data collection
cycle, being able to complete the survey in English or
Spanish, and having injected drugs within 12 months pre-
ceding the interview date as measured by self-report and
either evidence of recent injection or adequate description
of injection practices [10]. The survey measured charac-
teristics of participants’ IDU networks, demographics, drug
use and injection practices, sexual behaviors, HIV testing
history, and use of HIV prevention services. Participants in
NHBS-IDU2 were also offered an HIV test in conjunction
with the survey.
NHBS-IDU1 was conducted from May 2005 through
February 2006 in 23 cities. NHBS-IDU2 was conducted
from June 2009 through December 2009 in 20 cities, 18 of
which were included in NHBS-IDU1. Our results are based
on two cycles of data collection from 18 cities and 1 cycle
of data collection from seven cities, ﬁve from NHBS-IDU1
and two from NHBS-IDU2 (Fig. 1). For this analysis, RDS
data from each city are treated as independent samples.
Data from each city were analyzed separately. Results from
city level analysis are pooled and presented here by cycle.
In total 43 samples (23 samples from NHBS-IDU1 and 20
from NHS-IDU2) were included. Data collection time
varied across cities, due to differences in timing for human
subjects approvals, logistics, and speed of sampling.
All cities implemented a single protocol during each
cycle. Field operations across all cities were standardized
and followed common RDS procedures [24], however,
individual cities were provided ﬂexibility, such as deter-
mining the number of coupons given or interview locations
used, to meet local challenges. While not its primary pur-
pose, the presence of multiple simultaneous samples in the
NHBS-IDU research design provides a means for evalu-
ating RDS methodology when used to study populations at
increased risk for HIV.
To meet public health goals, NHBS focused on those
cites with the largest burden of AIDS disease based on
most recent available data at the time cites were being
AIDS Behav (2012) 16:797–806 799
123

4.
chosen: NHBS-IDU1 cities were chosen based on AIDS
data available from 2000; NHBS-IDU2 cities were chosen
based on AIDS data available from 2004 [23]. As such, the
cities are not necessarily representative of all U.S. cities or
IDU populations. However, NHBS cities are chosen to
ensure coverage of diverse geographic areas in the United
States and likely represent typical U.S. cities in which RDS
studies of IDU or other hard-to-reach populations would be
conducted.
Measures
Efﬁciency of network-based samples, such as RDS, is
correlated with homophily in the social network [4].
Homophily is the network principal that similar individuals
are more likely to form social connections than dissimilar
ones.
In networks where members are deﬁned by a speciﬁc
stigmatized activity—such as injection drug use—the
highest homophily variables tend to be basic demographic
characteristics [25]. Based on formative research, we
identiﬁed three key NHBS-IDU variables likely to have
high homophily and, consequently, the largest cDEs: race/
ethnicity, gender, and age. Race and Hispanic ethnicity
were asked separately, then coded into one variable with
mutually exclusive categories: white, black, Hispanic
(regardless of race), and other (including American Indian
or Alaska Natives, Asian, Native Hawaiian and Paciﬁc
Islander, and multiracial). Gender was coded as male or
female. Age was grouped into ﬁve categories: 18–24,
25–29, 30–39, 40–49, 50 years and over. In addition, we
analyze cDE for two variables related to HIV risk: sharing
syringes and self-reported HIV status. Sharing syringes was
deﬁned as having shared any syringes or needles in the past
12 months. Self-reported HIV status was coded as HIV-
positive or not (HIV-negative, indeterminate results or
status, never received the result, never tested).
Data
As shown in Fig. 2, during the NHBS-IDU1 and NHBS-
IDU2 a total of 26,705 persons were recruited to participate
(13,519 in NHBS-IDU1; 13,186 in NHBS-IDU2), 524 of
whom were seeds (384 in NHBS-IDU1; 140 in NHBS-
IDU2). The target sample size for each city in each cycle
was 500 IDU (range: 186–631 IDU).
In NHBS-IDU1, a total of 1,563 (12%) persons did not
meet NHBS-IDU eligibility criteria and were excluded
from analysis. An additional 46 persons had no recruitment
information and their records were also excluded. Among
the 11,910 eligible persons, we retained only recruitment
data for 439 (3.2%) persons whose survey data were either
lost during data transfer (334), who were not identiﬁed as
male or female (67) or whose responses to survey questions
were invalid (38). The purpose of this analysis procedure is
Fig. 1 Map of NHBS-IDU1 and NHBS-IDU2 sampling sites by participating cycle. Cycle for which data are available is shown in parenthesis
next to city names. If no cycle is shown, data are available for both NHBS-IDU1 and NHBS-IDU2
800 AIDS Behav (2012) 16:797–806
123

5.
to maintain recruitment chains. Their survey data were
coded as missing thus excluded from analysis.
In NHBS-IDU2 a total of 2,692 (20.4%) persons were
screened ineligible. These included 2,687 persons who did not
meet NHBS-IDU eligibility criteria and 5 persons without
recruitment information. Among the 10,494 persons included
in the analysis, 279 persons (2.7%) were included with only
recruitmentdatainordertomaintainrecruitmentchains.These
include 142 lost records, 55 persons who were not identiﬁed as
male or female, 64 persons with incomplete survey, and 18
persons forother reasons(repeated participants, invalid survey
response or invalid participation coupons).
The ﬁnal analysis sample included 21,686 persons
(11,471 for NHBS-IDU1 and 10,215 for NHBS-IDU2),
including seeds. As this analysis does not present test
results, participants with missing or indeterminate HIV test
results were not excluded from the analysis. Raw sample
proportions (unweighted), aggregated national estimates
(weighted), and median homophily for all ﬁve analysis
variables are presented in Table 1.
Analysis Techniques
As discussed above, measurement of cDE requires a means of
calculating variance. Several methods of estimating RDS
variance have been presented [5, 18, 19, 26]. A detailed dis-
cussion of these estimates is beyond the scope of this paper.
For this analysis, Salganik’s [18] bootstrap variance estimate
procedure was used for two reasons. First, this paper revisits
Salganik’s [18] recommendation that cDE = 2 should be used
in calculation of RDS sample size. Our use of the same vari-
ance estimation provides a consistent comparison. Second,
this is the variance estimate employed by RDS Analysis Tool
(RDSAT). To date, RDSAT is the only RDS analysis software
publically available. While multiple RDS variance estimators
have been proposed, RDSAT remains the primary RDS
analysis option for most researchers not involved in the
development of new estimators.
RDS analysis was conducted using RDSAT 8.0.8 with
a = 0.025 and 10,000 resamples for bootstrapping to cal-
culate estimates and estimate standard errors [27]. cDEs
were calculated as the ratio of RDS variance to variance
expected under SRS, as deﬁned above. cDEs were calculated
independently for each variable within each city. Observed
cDEs for each variable across all cities within a given cycle
are presented in each box plot. A tall box plot represents
large variation in cDE across cities. Homophily was calcu-
lated in RDSAT using Heckathorn’s formula [4, 24]:
Ha ¼
Saa À cPa
1 À cPa
if Saa ! cPa
Ha ¼
Saa À cPa
cPa
if SaacPa
ð3Þ
where Ha is homophily of subgroup a, Saa is the proportion
of in-group recruitments of individuals in subgroup a, and
cPa is the estimated proportions of a individuals in the
population. As deﬁned, homophily ranges from -1 to 1
Fig. 2 NHBS-IDU1 and NHBS-IDU2 analysis data
AIDS Behav (2012) 16:797–806 801
123

7.
50% of the variation in cDE attributable to differences in
homophily. The intercept in both models, 2.55 in IDU1 and
2.73 in IDU2, is above 2. Thus, even when there is no
homophily, the expected cDE is greater than the current
recommendation. A linear ﬁt was tested but ruled out when
the residuals plots showed non-random clear patterns
suggesting a non-linear ﬁt. A non-linear association is
consistent with Heckathorn [4] who hypothesizes a non-
linear association between homophily and standard error in
RDS studies.
Discussion
Our results show that while RDS cDEs tend to vary by city
and analysis variable, the majority of cDEs fall between
cDE = 2 and cDE = 4 with the exception of several race
categories. As mentioned above, the NHBS populations
Fig. 3 cDE by gender, self-reported HIV status, and syringe sharing
behavior for two cycles of NHBS-IDU. cDEs of dichotomous variables
are equivalent across category (i.e. cDE of males = cDE of females)
Fig. 4 cDE of estimates for age of IDU in NHBS-IDU1 and NHBS-
IDU2
Fig. 5 cDE of estimates of race of IDU in NHBS-IDU1 and NHBS-
IDU2
Fig. 6 Association between cDE and homophily by gender, race, age,
HIV positive status and sharing syringes among IDU in 43 RDS
samples in NHBS-IDU1 and NHBS-IDU2. Poly (IDU1) and Poly
(IDU2) are the non-linear best ﬁt lines for NHBS-IDU1 and NHBS-
IDU2, respectively. Linear ﬁt lines were tested and ruled out when
residual plots showed clear nonrandom patterns
AIDS Behav (2012) 16:797–806 803
123

8.
tended toward insularity by race. Consistent with other
work [4], we found high cDEs were associated with high
homophily. High cDEs for blacks, Hispanics, and whites are
likely due to the high homophily we observed for these
groups.
Our results support two conclusions. First, the original
cDE recommended by Salganik [18] for use in sample size
calculations of RDS studies ( cDE = 2), is unlikely to pro-
vide adequate statistical power in RDS studies of IDU in the
U.S. While some cDEs at or below two were observed, the
vast majority fell above cDE = 2. Second, with the excep-
tion of estimates of race, cDEs tended to fall below cDE = 4.
Coupled with our previous assessment that these data can be
viewed as representative of typical RDS studies of IDU in
the U.S., the results suggest that cDEs for successful RDS
studies focusing on this population will generally fall in the
range of two to four. Consequently, we recommend
cDE = 4 as a more appropriate, realistic estimate of cDE to
use when calculating sample size requirements for RDS
studies of IDU in the U.S. In multi-racial studies, formative
research should be conducted to determine the level of
racial homophily within the population. If race homophily
is too high, separate studies maybe necessary.
Calculating Sample Size
Based on our ﬁnding that cDE = 4 is a more realistic
estimate for the cDE of RDS studies of U.S. IDU popula-
tions, we can now calculate sample size estimates for
future research using Eq. 1 For example, if based on pre-
existing knowledge we suspect approximately 30% of IDU
engage in a high-risk behavior and we want to estimate this
prevalence with a standard error no greater than 0.03, the
required sample size is calculated as follows:
n ¼ 4 Á
ð0:3Þ 1 À 0:3ð Þ
0:03ð Þ2
¼ 933 ð4Þ
Thus, we would need a sample of 933 IDU in our study.
Note that while the relationship between sample size and
DE is linear, the relationship between sample size and
standard error is exponential. Therefore, while achieving
the same statistical power requires a sample size four times
larger than SRS, an RDS study with sample size similar to
SRS will reduce statistical power by less than four times.
For example, if we make the above estimate with a desired
maximum standard error of 0.04 instead of 0.03 the new
sample size requirement is:
n ¼ 4 Á
ð0:3Þ 1 À 0:3ð Þ
0:04ð Þ2
¼ 525 ð5Þ
By slightly reducing statistical power (i.e., increasing
maximum standard error), we reduce the required sample
size by about 50% to 525 IDU. Figure 7 shows the
relationship between sample size and standard error of
estimates for RDS studies with cDE = 4 for Pa = 0.3 and
Pa = 0.5. The relationship is exponential, so a reduction in
standard error from 0.03 to 0.04 provides a greater
reduction in absolute sample size than reducing standard
error from 0.04 to 0.05. A population proportion estimate
of 0.5 (Pa = 0.5) provides the most conservative estimates
and should be used in the absence of outside information.
Researchers faced with limited resources may be willing to
accept higher standard errors to keep sample size
requirements low. Additionally, because cDE = 4 is a
conservative estimate, studies planning for higher standard
errors may ﬁnd observed standard errors are lower than
initially expected for many variables.
Conclusion
Our analyses suggest that a cDE of four ( cDE = 4) is pre-
ferred for applying to calculations of sample size for future
RDS studies of IDU in the U.S. This cDE is higher than
Salganik’s [18] estimate, but lower than some recent the-
oretical estimates suggested by Goel and Salganik [20].
The advantage of our recommendation is its empirical basis
and practical emphasis.
Our results are likely generalizable to RDS studies of
IDU populations in the U.S. Studies of non-IDU or popu-
lations outside the U.S. should apply our results with
caution. Second, our data originate from larger urban
populations. Studies of rural IDU may ﬁnd different cDE
outcomes. Third, while the large number of cities included
in NHBS covers a wide range of IDU populations, city
selection favored those cities with the highest overall HIV
100
300
500
700
900
1100
1300
0.03 0.035 0.04 0.045 0.05 0.055 0.06
RequiredSampleSize
Maximum Standard Error
Pa=.5, DE=4
Pa=.3, DE=4
Fig. 7 Required sample size decreases sharply as the maximum
allowable standard error of estimates is relaxed for samples with cDE
of four based on Eq. 1 Pa is the population proportion of individuals
with characteristic ‘a’
804 AIDS Behav (2012) 16:797–806
123

9.
burden. Thus, results from studies conducted in cities with
lower HIV burdens may differ from our own.
Beyond generalizability, our results have several limi-
tations. First, our recommendation that cDE = 4 should be
used in estimating sample size requirements may underes-
timate cDE with respect to race. The large cDEs we observed
are likely due to higher homophily by race than other
variables, a common ﬁnding in U.S. populations. Fortu-
nately, racial homophily is relatively easy to monitor during
sampling and to address in formative research. If racial
homophily is too high, stratiﬁed results can be reported by
race. If racial homophily is excessive, such that almost no
cross-race recruitment is observed, samples can be sepa-
rated by race and analyzed separately. If external informa-
tion on the relative size of the different racial groups is
available, the results can be aggregated. Second, our anal-
ysis relies on conﬁdence interval bounds generated using
the RDSAT bootstrapping algorithm [18, 27]. The algo-
rithm is not the only method of calculating RDS variances
[5, 19, 26] and has been shown to underestimate variance
under certain conditions [20, 21]. If this variance estimation
procedure is not correct the true DE could be higher, pos-
sibly much higher, than cDE. Similarly, as new, more efﬁ-
cient estimation procedures are developed a lower cDE may
become more appropriate for estimating sample size.
Unfortunately, there is often a signiﬁcant delay between
when new methods are developed and when they are
accessible for use by the scientiﬁc community. For practi-
cality, we chose a variance estimate that is most accessible
to researchers utilizing RDS today. Third, differences in
implementation within city across time, such as the number
of coupons given to each respondent, negate our ability to
explore the effect of some implementation differences on
cDE. Fourth, our analysis focused on the relationship
between cDE and homophily, a trait level measure of clus-
tering. Recent work suggests bottlenecks may be a more
appropriate level of analysis than homophily [20]. Bottle-
necks are a function of the entire network structure and how
traits are distributed across that network structure. Unfor-
tunately, our RDS data do not provide sufﬁcient information
to analyze the global network structure. Based on the lit-
erature, we expect the association between cDE and bottle-
necks to be stronger than the association between
homophily and cDE. Finally, this analysis analyzed data
from 43 RDS samples implementing a standard NHBS
protocol. While the NHBS protocol follows standard RDS
procedures and allowed ﬂexibility meet the unique condi-
tions of each city, it is possible that different studies could
yield different results. This is especially true of studies that
use modiﬁed RDS procedures. Further research is needed to
explore the effect of differences in implementation on cDE.
Despite these limitations, we argue that these results
provide an alternative to earlier recommendations for cal-
culating RDS sample size in studies of U.S. IDU and serve
as a guide to researchers planning future RDS studies.
Previous research presenting weighted RDS estimates and
conﬁdence intervals is not impacted by our results, as
conﬁdence intervals from RDS analysis account for DE.
However, our results further highlight the need for con-
ducting RDS analysis on RDS data. Unweighted analyses
of RDS data, which treat the sample as an SRS, not only
risk presenting biased estimates, but also risk underesti-
mating the variance of those estimates by as much a factor
of four.
Public health researchers working with RDS data will
beneﬁt from our results by ensuring they have adequate
power for identifying health outcomes such as HIV prev-
alence and/or risk behaviors. Data collections, including
NHBS-IDU, must balance the need for precise estimates
with the need to limit burden on the public and to ensure
the best use of limited resources. The current target sample
size for NHBS-IDU is 500 per city and 10,000 nationally.
This sample size is adequate for national estimates, but
may have limited power locally for some variables of
interest. Given the exponential relationship between stan-
dard error and sample size, researchers may be willing to
accept and plan for higher standard errors to keep sample
size requirements low.
Acknowledgments This paper is based, in part, on contributions by
National HIV Behavioral Surveillance System staff members,
including J. Taussig, R. Gern, T. Hoyte, L. Salazar, B. Hadsock,
Atlanta, Georgia; C. Flynn, F. Sifakis, Baltimore, Maryland; D.
Isenberg, M. Driscoll, E. Hurwitz, Boston, Massachusetts; N. Prac-
hand, N. Benbow, Chicago, Illinois; S. Melville, R. Yeager, A.
Sayegh, J. Dyer, A. Novoa, Dallas, Texas; M. Thrun, A. Al-Tayyib,
R. Wilmoth, Denver, Colorado; E. Higgins, V. Grifﬁn, E. Mokotoff,
Detroit, Michigan; M. Wolverton, J. Risser, H. Rehman, Houston,
Texas; T. Bingham, E. Sey, Los Angeles, California; M. LaLota, L.
Metsch, D. Beck, D. Forrest, G. Cardenas, Miami, Florida; C. Ne-
meth, C.-A. Watson, Nassau-Suffolk, New York; W. T. Robinson, D.
Gruber, New Orleans, Louisiana; C. Murrill, A. Neaigus, S. Jenness,
H. Hagan, T. Wendel, New York, New York; H. Cross, B. Bolden, S.
D’Errico, Newark, New Jersey; K. Brady, A. Kirkland, Philadelphia,
Pennsylvania; V. Miguelino, A. Velasco, San Diego, California; H.
Raymond, W. McFarland, San Francisco, California; S. M. De Leo´n,
Y. Rolo´n-Colo´n, San Juan, Puerto Rico; M. Courogen, H. Thiede, N.
Snyder, R. Burt, Seattle, Washington; M. Herbert, Y. Friedberg, D.
Wrigley, J. Fisher, St. Louis, Missouri; and P. Cunningham, M.
Sansone, T. West-Ojo, M. Magnus, I. Kuo, District of Columbia.
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