Type 1 diabetes is an autoimmune disease resulting from the selective destruction of insulin-producing beta-cells. Beta cell culture for 6-9 days in the presence of IL-1 beta and interferon (INF) gamma leads to apoptosis. Primary rat beta-cells were FACS purified and exposed for 6 or 24 h to control condition, IL-1 beta + INF gamma, or IL-1 beta alone (24 h only). The gene expression profile was analyzed by hybridization in duplicate to the Affymetrix RG U34A microarray.