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Hematology Cellcounting - Basics PowerPoint PPT Presentation

Hematology Cellcounting - Basics. Hematology, is the part of internal medicine , that is concerned with the study of blood , the blood-forming organs, and blood diseases .

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Hematology Cellcounting - Basics

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Hematology, is the part of internal medicine,that is concerned with the study of blood, the blood-forming organs, and blood diseases.

The bunch of methods for counting, measuring and evaluating blood cells suspended in fluid is known as Hematology Cellcounting.

A blood cell analyzer, is used to perform one of medicine's most often-requested and informative diagnostic tests, the complete blood count (CBC).

Blood

Blood is a specialized fluid, „liquid tissue”, that circulatesaround the body in blood vessels.

It’s main functions:

- Transport through the body of

oxygen and carbon dioxide

nutrient molecules, ions, hormones

„wastes”

Heat

- Defense of the body against infections, injuries and other foreign materials.

Composition of blood

LiquidPlasma: over 90% of plasma is water, it also contains proteins, salts, metabolits etc. After centrifuging it is called serum.

Cells:Erythrocytes(Red Blood Cells, RBC)

Leukocytes(White Blood Cells, WBC)

Thrombocytes(Platelets, PLT)

Erythrocytes The most numerous and common type of blood cells. Non nucleated in mammals, with biconcaveshape, erythrocytes are responsible for the transport of oxygen. Contain hemoglobin(HGB), iron, and structures on the cell membranedetermine blood group systems.

Erythrocytes – cont’d

Reticulocytes: immature blood cell forms, contain remnants of the nucleus(ribosomal RNA). Present normally in a very few% of the total RBC number.

Thrombocytes and other parameters

Thrombocytes: thrombocytes or platelets play an important role in the cellular partof the hemostazis(coagulation) trying to prevent excessive blood loss. Non nucleated cells in mammals and the smallest ones inthe same time. Containspecificgranules.

Hematocrit – HCT or Packed Cell Volume – PCV:Shows the volume of blood in % occupied by cells.It can be measured after separation in a centrifuge, or calculated after cell counting. Shows the degree of anemia.