1 1C genome size and n chromosome number for bryophytes.2 Position refers to that of most chromosomes in the species and can be either interstitial (i), (peri-)centromeric (c) and (sub-)terminal or satellite (t).3 35S=45S=18S-5.8S-26S rDNA

Protocols, reagents and checklists for rDNA fluorescent in situ hybridisation in plants

Here you will find:

A simplified method for fluorescent in situ hybridisation on plant protoplasts based on the one proposed by Heslop-Harrison et al. (1991) with minor modifications by Dr. J. Siroky (as explained in Garcia et al., 2010) and Siljak-Yakovlev et al. (in press) and some adaptations of our own. PDF 1

A checklist of the basic laboratory equipment, lab tools, consumables and reagents for fluorescent in situ hybridisation in plant protoplasts. PDF 2

A method for obtaining plant protoplasts, based on Geber and Schweizer (1988) with minor modifications from Siljak-Yakovlev et al. (in press) and some adaptations of our own. PDF 3

A complete description of the necessary reagents both for in situ and for obtaining plant protoplasts. PDF 5

A couple of protocols for probe labelling by nick translation, following the manufacturer’s instructions. These include a calculator on the necessary amount of DNA probe for each labelling reaction. XLSX 1

We wish to thank Dr. Sonja-Siljak Yakovlev (Université Paris Sud – CNRS) and Dr. Jiri Siroky for sharing their knowledge, experience and advice on these techniques, as well as on the assessment and the preparation of these documents.