A study of protein synthesis in damaged mitochondria

Summary

Experiments were performed with mitochondrias lyzed in distilled water. It was demonstrated that mitochondrias have to be intact for formation of the cofactor required for the protein biosynthesis in vitro. Isolated intact mitochondrias are able to synthesize protein without any addition of a previously obtained cofactor.

Destructed mitochondrias as are able to synthesize protein only with the addition of a cofactor obtained from intact mitochondrias. Treatment with crystalline ribonuclease results in the inhibition of the protein synthesis both by intact and destructed mitochondrias.