Application Information: OTUB2 antibody [2B2]

WB 1:2000 Flow Cytometry 1:100Optimal working dilutions should be determined experimentally by the end user.

Form Supplied

Liquid

Purification

Protein A/G purified

Specifications: OTUB2 antibody [2B2]

Full Name

OTU domain, ubiquitin aldehyde binding 2

Background

Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.

Synonyms

MGC3102, C14orf137, OTB2, OTUB2, FLJ21916, OTU2

Specificity

GTX83956 is specific for human OTUB2 (NP_075601).

Host

Mouse

Clonality

Monoclonal

Clone Name

2B2

Isotype

IgG1

Immunogen

Full length human recombinant protein of human OTUB2 (NP_075601) produced in HEK293T cell.

Antigen Species

Human

Species Reactivity

Human

Conjugation

Unconjugated

Storage Conditions: OTUB2 antibody [2B2]

Storage Buffer

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage Instruction

For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles.

Notes

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OTUB2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OTUB2.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OTUB2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OTUB2.