Cris
We have always been successful cloning PCR fragments into a vector
described by Holton and Graham. Holton, T.A. and Graham, M.W. (1991) A
simple and efficient method for direct cloning of PCR products using
ddt-tailed vectors. Nucleic Acids Research, 19:1156.
Jerry Forrest
Department of Biology
Beckman Research Institute/City of Hope
Duarte, CA