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The spruce is the one of the refractory wood species. In this study, the spruce sapwood samples were treated with enzymes to improve its permeability. The pit membranes play an important role for water transporting between the adjacent cells. The spruce wood pit membranes are prone to close under the fiber saturated point. As a result of the liquid transportation is blocked, the impregnation process is getting difficult. The wood pits compose of cellulose, hemicellulose, lignin, pectin, and phenolic components. The torus surface is covered with pectin. Bioprep 3000 L, Viscozyme L, Texazym BFE, Texazym DLG are commonly used in the textile industry to remove lignocellulosic materials. It was aimed to destroy the closed pits via enzymatic treatment. At the end of the study, the retention and penetration increments were obtained after the enzymatic treatment. The wood samples treated with enzymes which are composed of enzymes mixtures gave more penetration and retention values.