アプリケーション

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション

Abreviews

特記事項

ChIP

Use at an assay dependent dilution. PubMed: 19074263

WB

Use at an assay dependent dilution. Preliminary experiments gave an approx 52kDa band in K562 cell lysate after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of 38kDa according to NP_006554. The 52kDa band was successfully blocked by incubation with the immunizing peptide

ターゲット情報

機能

Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.

Defects in KLF1 are the cause of congenital dyserythropoietic anemia type 4 (CDA4) [MIM:613673]. It is a blood disorder characterized by ineffective erythropoiesis and hemolysis resulting in anemia. Circulating erythroblasts and erythroblasts in the bone marrow show various morphologic abnormalities. Affected individuals with CDA4 also have increased levels of fetal hemoglobin.

Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity.Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex.Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity.

画像

Detection of EKLF / KLF1 binding at the region upstream of the transcription start site of the HBE1 gene by ChIP with ab2483. Chromatin from CD43+ cells was cross-linked prior to ChIP using ab2483 at a concentration of 0.2 µg/µg chromatin. DNA was quantified using RT-PCR. Please see abreview by Genpathway Inc for additional details.

I have looked in this and it appears that the wrong image was placed on the Webpage for ab2483, however we do guarantee the antibody to work in ChIP, as the correct image can be found at the link below: Read More