Marvin the Martian appears in two Drawn Together episodes:Charlotte's Web of Lies (where he is seen in Ling-Ling's Anger Management Group with The Hulk, Skeletor, and Yosemite Sam) and Toot Goes Bollywood.

Speedy appeared in an episode trying to be a member of the housemates.

In The Drawn Together Movie: The Movie!, The Road Runner gets run down and dies. After The Road Runner's death, Wile E. Coyote (voiced by Jess Harnell) says that his life has no meaning without The Road Runner and then commits suicide by shooting himself in the head with a prop gun.

In the 7th JellyTelly DVD of Whats In the Bible? when Buck Denver says he was feeling peppy, Phil Vischer calls him "Peppy Lew Pew". Also during the outtakes when Phil made a mistake he studders and says "That's all folks".

He is mentioned in the Animaniacs segment Spellbound, in a spell Brain used throughout the episode.

In the Pinky & The Brain episode, Calvin Brain, he is mentioned by Pinky, when Brain says that they are going to a place where females are paid to change constantly. Pinky responds by asking whether this place is Charlie Sheen's House.

During the scene where The Riddler (Jim Carrey) demonstrates to Two-Face (Tommy Lee Jones) what his device is capable of on 2 of his henchwomen, the cartoons that pop up in front of their eyes are The Stupid Cupid and Satan's Waitin'.

Looney Tunes - Sniffles - the character was heavily based on Disney's Oscar-winning animated short, The Country Mouse

Animaniacs - Cutie & The Beast - an obvious parody of Beauty & The Beast, a then-recent Disney film based on the fairy tale of the same name

Tiny Toon Adventures - The show has parodied many Disney films and cartoons including Who Framed Roger Rabbit?, The Little Mermaid and Mickey Mouse Club. Roger Rabbit even made two cameo appearnces in the show.

In the episode "Die Sweet Roadrunner Die", Wile E. Coyote kills and eats The Road-Runner but realizes that he does not know what else to do in his life now. He tries working as a waiter but gets fired after having a mental breakdown. He is about to kill himself but then he becomes a Christian.