Jan Aelen (janal at nmr.kun.nl) wrote:
: Dear Netters,
: I want to express my protein in LB dissolved in deuterium-oxide instead
: of normal water.
: The host strain is BL21/DE3.
: Has anyone experience about the growth of the cells in this medium?
No. But why do you want to do this?
If you are thinking of labelling the protein with deuterium, LB would have
all the amino acids which would be preferentially incorporated
into your protein and you would therefore have a low level of
incorporation. Are you are thinking of labelling specific atoms or
residues?