NMYC gene amplification occurs in neuroblastoma. Less frequently amplifications are found in retinoblastoma, small cell lung cancer, astrocytoma and other tumors derived from the neuroectoderm. The NMYC (v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived) gene is located in the chromosomal region 2p24.3 and encodes a 62-64 kDa transcription factor mainly expressed in the developing nervous system. The BioGenex eFISH NMYC/2q11 Dual Color Probe is a mixture of a green fluorochrome direct labeled SPEC NMYC probe hybridizing to the human NMYC gene in the chromosomal region 2p24.3 and an orange fluorochrome direct labeled SPEC 2q11 probe specific for the AFF3 (AF4/FMR2 family, member 3) gene region in 2q11.2. Due to cross-hybridizations of chromosome 2 alpha satellites to other centromeric regions, probes specific for 2q11 are frequently used for chromosome 2 copy number detection.