Associated products

Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

ELISA

Use at an assay dependent dilution.

WB

Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 20 kDa).

IHC-P

1/100. (see Abreview)

Target

FunctionComponent of intercellular desmosome junctions. Plays a role in stratified epithelial integrity and cell-cell adhesion by promoting desmosome assembly. Plays a role as an effector in the TP53-dependent apoptotic pathway.

ab48032 staining PERP in human small intestine tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. An HRP-conjugated rabbit anti-goat polyclonal was used as the secondary antibody, diluted 1/200.

Thank you for your email and for keeping me updated about the situation. I'm sorry to hear that ab5986 did not work well with the mouse samples, and it is possible that this antibody just doesn't work with mouse. We have unfortunately removed ab39...

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