Prepare your students for the real world of scientific research. Engage them with the opportunity to perform novel, relevant research that can actually contribute to scientific knowledge. In this unique modular lab series, students are guided through an innovative research workflow identical to those performed in genomics labs worldwide. Over a multiple-week lab course, students will combine traditional and cutting edge molecular biology techniques and bioinformatics to clone, sequence, and analyze a housekeeping gene from a plant of your choice ensuring each class produces unique and novel data. The real thing. As a research and diagnostics manufacturing company, Bio-Rad brings unique strengths to the education market, including credibility, high quality products (kits and equipment that work), novel inquiry-based curricula, cutting edge kit applications, competitive pricing, superior teacher professional development programs, long-term strategic partners and allies, and strong personal endorsements from customers across all levels of education.

To clone a gene, genomic DNA (gDNA) must first be extracted from the organism. The nucleic acid extraction module allows students to extract all nucleic acids from plant cells using tissue grinding and lysis, followed by purification using column chromatography. The final sample, which contains gDNA and RNA, can be used for PCR. RNA can then be removed from the sample by treatment with RNase I as an optional activity.

The protocol provided with this module has been optimized for genomic DNA extraction from most plant species. Different plants yield different quantities of genomic DNA. Each extraction yields sufficient DNA for 16 PCR amplifications.

If you want students to use the nucleic acid extraction module to extract RNA, genomic DNA can be removed from the sample with DNase I treatment. For further RNA work, elution using DEPC-treated water is recommended.

Kit contains sufficient materials for 25 genomic DNA extractions

Lysis buffer

20 ml

Dithiothreitol (DTT)

0.3 g

Wash buffer

20 ml

Sterile water

2.5 ml

Micropestles

25

Spin columns

25

Microcentrifuge tubes

2.0 ml, 25

Microcentrifuge tubes

1.5 ml, 30

Colored microcentrifuge tubes

2.0 ml, 60

Instruction manual

1

What is the education discount policy?

For more than 15 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows high school and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices.

How do I apply for the education discount?

If you are an educator at the high school or college level, visit our Education Discount Policy page to establish an education account number.