Abstract

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF ONDONSETRAN USING REVERSE PHASE HPLC METHOD AND ITS APPLICATION TO DIFFERENT PHARMACEUTICAL DOSAGE FORMS

Authors

R. Vani, B.Vijaya Kumar, G. Krishna Mohan

Description

A new simple, accurate, precise and reproducible RP-HPLC method has been developed for the estimation of Ondonsetran in its bulk and different pharmaceutical dosage forms using inertsil ODS C18 column ( 250 x 4.6 mm, 5 µm) in isocratic mode. The mobile phase consists of 0.1MKH2PO4:ACN: MeOH (30:40:30)v/v pH6.0 . The detection was carried out at 216 nm for Ondonsetran. The method was linear over the concentration range 48-112 µg/ml. The validation of method was carried out utilizing ICH-guidelines.