Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

WB

ICC/IF

IHC-P

Application notesWB: Use at a concentration of 1-5 µg/ml.

Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Target

FunctionInduces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.

Tissue specificityExpressed in the kidney and bladder. Lower levels seen in the brain.

Sequence similaritiesBelongs to the TGF-beta family.

Developmental stageExpressed in the developing eye, brain and ear during embryogenesis.

Post-translationalmodificationsSeveral N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms (PubMed:17977014).

Anti-BMP7 antibody images

IHC image of BMP7 staining in Human Kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab54904, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence - Anti-BMP7 antibody (ab54904)This image was taken from an abreview submitted by Ruma Raha-Chowdhury.

ICC/IF image of ab54904 staining of BMP7 in rat primary hippocampal neuron culture. The sections were incubated in 0.3% triton X to permeabilise the cells and in 10% serum to block non-specific protein-protein interactions. The sections were incubated with ab54904 (1:1000) overnight at +4°C, followed by a second incubation with Alexa 488 conjugated secondary antibody. BMP7 (green staining) is observed in the pyramidal cells of the hippocampus.