transcription factors, splicing factors, or RNA-decay machinery ( 17 , 18 ). In this study, we aimed to identify lncRNA–mRNA pairs that play a functional role in GD.
We found that patients with newly diagnosed GD had significantly higher numbers of B cells

expression of VDR mRNA in malignant thyroid tissues is higher than that in normal thyroid ( 2 ). They also reported correlations between VDR and other genes such as ECM1 (extracellular matrix protein-1) and TMPRSS4 (type II transmembrane serine

D A Dart, K Ashelford and W G Jiang

to the AR mRNA transcript and may be in part responsible for increased AR mRNA and protein expression in response to AR-antagonist-mediated hormone starvation.
Materials and methods
Cell culture
LNCaP cells were maintained at 37°C, 5% CO

. Unlike most cells whose mRNA transcripts lasts for only a few hours ( 50 ), an oocyte transcribes mRNAs to last several days ( 50 ) from fertilization to blastulation and implantation ( 50 ). The longevity of these mRNA transcripts in this unique

the PCR products were verified by agarose gel electrophoresis (data not shown). mRNA abundance was constructed using a standard curve of the calibrator sample (a term placental sample collected at elective Caesarean section), and abundance was