Application Information: CD15 antibody [28]

FACS: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. Note: samples should be acetone fixed. IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

Concentration

1
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

IgG fraction

Specifications: CD15 antibody [28]

The CD15 antigen is expressed on approximately 90% human circulating granulocytes (membranes and granules), 30 to 60% of circulating monocytes and is absent from normal lymphocytes. CD15 antigen is also expressed on Reed Sternberg cells of Hodgkin's disease, on T cell lymphomas including mycosis fungoides and on some leukemias. Outside the hematopoietic system, CD15 expression is described in certain normal and neoplastic epithelial cells and in astrocytes. CD15 antibodies recognize the tri saccharide 3 fucosyllactosamine (3Fl) which is present in lacto N fucopentaose III and in the blood group antigen X hapten. At least 5 major CD15 antigens (105, 135, 165, 185, 220 kDa) are present on the surface membranes of polymorphonuclear cells. The hapten occurs also in glycolipids. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis.