应用

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用

Ab评论

说明

Flow Cyt

Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr

Use at an assay dependent concentration. Used at a dilution of 1/200 for 1 hr on mouse aortic root (see Abreview for further details).

IHC-P

1/400.

WB

1/200. Predicted molecular weight: 95 kDa.

The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 kDa to 160 kDa on SDS-PAGE.

ICC

1/100.

靶标

功能

Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.

疾病相关

Defects in LDLR are the cause of familial hypercholesterolemia (FH) [MIM:143890]; a common autosomal semi-dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis).

ab30532 staining LDL receptor in human lung parenchyma.Left panel: with primary antibody at 1/400. Right panel: isotype control.Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

ICC/IF image of ab30532 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30532, 1/400) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

As we discussed, I will send you a positive control free of charge: ab7187 (RAW 264.7 whole cell lysate). This cell lysate gave a dark lower molecular weight band, the identity of which is unknown, along with bands at 110, 130, and 160 kDa corresp...

Thanks for your email. I believe this replacement order has been shipped and delivered on Tuesday July 18. It was on Abcam order reference number 1128916 which contained a vial of ab61392. I hope this is helpful. Please let me know if you hav...

I have attached a western blot image showing that depending on the sample type that you are probing, you will get a number of different bands. Therefore, when running your western blot, you should expect to see ...

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab61392 and one vial of ab52818.

Thank you for sending along the additional PO information. I have located that order for ab61392 on our system. I am happy to send out the replacement antibodies. Since a couple options have been discussed for these replacement antibodies, I just ...

Thanks for your reply. Again I apologize for the trouble finding antibodies to 3-nitrotyrosine & the human LDL receptor. The antibodies you have ordered so far have very few complaints and are ordered relatively frequently so it is certainly an un...