Overview

Macrophage colony-stimulating factor (M-CSF) is a homodimeric glycoprotein growth factor that regulates proliferation and differentiation of myeloid hematopoietic progenitors to mononuclear phagocytic cell lineages, including monocytes, macrophages, and osteoclasts. M-CSF is a crucial factor for the development of tissue-resident macrophages in most tissues (Ginhoux & Jung). It is required for the maturation and activation of monocytes and macrophages, and regulates inflammatory responses in conjunction with other stimuli such as IFN-γ, LPS, and IL-4 (Murray et al.). M-CSF is also required for bone resorption by osteoclasts, and is involved in the development and regulation of placenta, mammary gland, and brain. M-CSF is produced by monocytes, fibroblasts, osteoclasts, stromal cells, endothelial cells, and tumor cells (Chockalingam & Ghosh).

Product Applications

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Data and Publications

Data

(A) The biological activity of Human Recombinant M-CSF, ACF was tested by its ability to promote the proliferation of NFS-60 cells. Cell
proliferation was measured after 62 hours of culture using a fluorometric assay method. The EC50 is defined as the effective
concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 3 ng/mL.
(B) 1 μg of Human Recombinant M-CSF, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and
visualized by Coomassie Blue staining.

Quality Statement:

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