The glycine-tyrosine-phenylalanine (GYF) domain is an around 60-amino acid
domain which contains a conserved GP[YF]xxxx[MV]xxWxxx[GN]YF motif. It was
identified in the human intracellular protein termed CD2 binding protein 2
(CD2BP2), which binds to a site containing two tandem PPPGHR segments within
the cytoplasmic region of CD2. Binding experiments and mutational analyses
have demonstrated the critical importance of the GYF tripeptide in ligand
binding. A GYF domain is also found in several other eukaryotic proteins of
unknown function [9843987]. It has been proposed that the GYF domain found in these
proteins could also be involved in proline-rich sequence recognition [10404223].
Resolution of the structure of the CD2BP2 GYF domain by NMR spectroscopy
revealed a compact domain with a beta-beta-alpha-beta-beta topology, where the
single alpha-helix is tilted away from the twisted, anti-parallel beta-sheet.
The conserved residues of the GYF domain create a contiguous
patch of predominantly hydrophobic nature which forms an integral part of the
ligand-binding site [10404223]. There is limited homology within the C-terminal 20-30
amino acids of various GYF domains, supporting the idea that this part of the
domain is structurally but not functionally important [12426371].

Browse genome assignments for this superfamily. The SUPERFAMILY hidden Markov model library has been used to carry
out SCOP domain assignments to all genomes at the superfamily level.

Alignments of sequences to 2 models
in this superfamily are available by clicking on the 'Alignments' icon above. PDB sequences less than 40% identical
are shown by default, but any other sequence(s) may be aligned. Select PDB sequences, genome sequences, or paste in or upload your own sequences.

Browse and view proteins in genomes which have
different domain combinations including a GYF domain domain.

Examine the distribution of domain superfamilies, or families, across the major taxonomic kingdoms or genomes within a kingdom. This gives an immediate impression of how superfamilies, or families, are restricted to certain kingdoms of life.

There are 2 hidden Markov models representing the GYF domain superfamily. Information on how the models are built, and plots showing hydrophobicity, match emmission probabilities and insertion/deletion probabilities can be inspected.