Аннотация: TH112 Phospholipid fatty acid profiles as indicators of microbial
community composition variations in soil by mixed heavy metal
pollution M. Pukalchik, Lomonosov Moscow State University /
Faculty of Soil Science; V. Terekhova, Institute of Ecology and
Evolution RAS / Lab Ecologicall Soil Functions; E.V. Fedoseeva,
Pirogov Russian National Research Medical University /
Medicobiological faculty; K. Kydralieva; O. Yakimenko, Moscow State
University / Soil Science Faculty; N. Verkhovtseva, Lomonosov
Moscow State University (MSU) / Faculty of Soil Science. Primary
information on heavily contaminated urban areas comes from many
cities, however, chemical pollution assays are unable to evaluate the
integrated biological effect, which shows a ‘cocktail’ of environmental
factors acting simultaneously on biological systems. This study focuses
at assessing of the microbial community structure of urban soils by
phospholipid fatty acid analysis (PLFA). The soil samples(topsoil layer
0-20 cm depth) were taken at six sites located in the rural part of the
cities of Kirov (Russia), differently polluted with heavy metals.The
samples were measured for pH and the contents of organic C, total N,
total P and total Pb, Ni, Cr and Cd. The structure of soil microbial
communities was assessed using PLFA analysis (high-performance
liquid chromatography–mass spectrometry method).The total PLFA
content in control sample were found to be significantly higher than the
polluted soil. The greatest indication value was given to an
Actinobacteria phylum, their concentration decrease remarkably in the
polluted samples, and anaerobes Butyrivibrio sp. and Bifidobacterium
sp.were found as indicator of soils under relatively low soil pollution
status.The microbial profileswere also indicated a selective enrichment
of competent species (Desulfovibrio sp., Bacteroides fragilis, Chlamydia
sp., C. trachomatis) in soil with high heavy metals contamination.
Nonmetric multidimensional scaling plots of soil communities, showing
the relative differences in control and pollution soils.Overall, our
studycould promote to better understand how microbial community
structure might adapt to heavy-metals stress. This study is supported by
RSF (14-50-00029).