AAV Control Products

Which control AAV should I use?

There are three primary factors to take into consideration when selecting the control AAV: the AAV serotype, the promoter, and the reporter. Ideally, you would use the control with the same serotype and the same promoter as the AAV expressing your GOI/shRNA/sgRNA. For example, if you use AAV5-CAG-hSNCA for stereotactic injection, you would need a control AAV with serotype 5 and the CAG promoter to drive the fluorescence reporter (eGFP, mCherry, etc). In this case, most labs would select AAV5-CAG-eGFP.

For shRNA or miRNA, a control AAV expressing a scrambled shRNA/miRNA which targets no known mammalian genes is also needed.