Properties for Influenza B NCP Antigen Capture ELISA - Photometric

Datasheet Extract

Background

Influenza viruses can be divided into three classes, A, B, and C, largely based upon conserved antigenic differences in the internal nucleoprotein. Influenza A virus, typically encountered more frequently than types B and C, and associated with the majority of serious epidemics, can be further subdivided into strains or subtypes based on antigenic differences in the external hemagglutinin proteins (H1-H16) and neuraminidase proteins (N1-N9).

Influenza B virus is predominantly a human pathogen, although it has been found to infect seals. The limited host range of influenza B and a slower rate of mutation than influenza A appears to preclude development of influenza B pandemics, but influenza B is a significant human pathogen and on an individual basis, infection may result in death.

While Influenza A subtype identification is extremely important (vaccine production, epidemiology), the rapid and accurate differentiation of influenza B from influenza A and C and other respiratory agents in humans and animals is also important (treatment and biosecurity). Virusys has developed a highly sensitive and specific enzyme immunoassay for the detection of Influenza B nucleoprotein antigen in complex sample matrices. The assay can be completed in less than 1.5 hr. and contains only one wash step.

The Virusys Influenza B Nucleoprotein Antigen Detection ELISA also incorporates proprietary diluents that are designed to prevent the development of nonspecific signal derived from complex sample matrix effects or the nonspecific adsorption of reactive test components resulting in both high sensitivity and specificity. The test kit is available in a standard photometric format that utilizes TMB (3,3',5,5'-tetramethylbenzidine), a highly sensitive, photometric horseradish peroxidase (HRP) substrate.

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