I guess I'm always puzzled by using different types of FBS (that is, regular vs dialyzed vs heat-inactivated FBS) in different cell line types. Could someone help to clarify this concept for me as when should be particular using the dialyzed and heat-inactivated sera? Additionally, with the GS (glutamine synthetase) system media (http://www.jrhbio.com/Document.aspx?ID=225), why is it using the dialyzed FBS in its recipe? Many thanks in advance!!

-bhchen66-

QUOTE (bhchen66 @ Nov 22 2005, 12:49 AM)

Dear all:

I guess I'm always puzzled by using different types of FBS (that is, regular vs dialyzed vs heat-inactivated FBS) in different cell line types. Could someone help to clarify this concept for me as when should be particular using the dialyzed and heat-inactivated sera? Additionally, with the GS (glutamine synthetase) system media (http://www.jrhbio.com/Document.aspx?ID=225), why is it using the dialyzed FBS in its recipe? Many thanks in advance!!

Hi

Did you get any answers regarding this Q? Just interested. If yes please share.

Thanks,

P

-padma_dp-

QUOTE (padma_dp @ Nov 23 2005, 06:17 AM)

QUOTE (bhchen66 @ Nov 22 2005, 12:49 AM)

Dear all:

I guess I'm always puzzled by using different types of FBS (that is, regular vs dialyzed vs heat-inactivated FBS) in different cell line types. Could someone help to clarify this concept for me as when should be particular using the dialyzed and heat-inactivated sera? Additionally, with the GS (glutamine synthetase) system media (http://www.jrhbio.com/Document.aspx?ID=225), why is it using the dialyzed FBS in its recipe? Many thanks in advance!!

Hi

Did you get any answers regarding this Q? Just interested. If yes please share.

Thanks,

P

Not yet. But if I do, I'll certainly post it back here. :-)

-bhchen66-

Here's what I found out about dialyzed sera. In particular if you're doing a media selection for the GS system, you would always want to eliminate the trace amounts of glutamine present in the sera. So, dialyzing FBS will help get rid of that in this case.