Monocytes of human and different animals as well as soluble human CD14.

Host:

Mouse

Reactivity:

Human

Specificity:

biG 4 represents an excellent marker for CD14. CD14 has been shown to be involved in the LPS-induced cell activation. This antigen is present as a membrane bound form and as soluble antigen, both involved in cell stimulation by LPS. The plasma protein LBP plays an important role in this LPS-CD14 mediated cell activation. Monocytes and PMN are activated by the LBP-LPS complex via membrane CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin.

Form:

Lyophilized

Isotype:

IgG1

Recommend Usage:

The optimal working dilution should be determined by the end user.

Storage Buffer:

Lyophilized from PBS

Storage Instruction:

Store at -20°C on dry atmosphere.After reconstitution with deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.

CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq