Roadblock

The Big Guy: Shared with Snake Eyes and Heavy Duty from the first movie.

Composite Character: His background has several elements from Stalker in the comics, such as being involved with a gang when growing up in the city, being best buddies with Snake Eyes, and having kids.

Dressing as the Enemy: Disguised herself as a Cobra red ninja to get close to the Cobra Commander, Firefly and Zartan along with Storm Shadow, who was going after Zartan because he murdered the Hard Master.

The Quiet One: Though she has a few lines, unlike Snake Eyes, she's relatively taciturn.

Grunt

Clutch

Stoop

MARS/Cobra

The Doctor/ Cobra Commander

One half of the Big Bad Duumvirate of the first film, which makes sense because he's the Big Bad of the franchise. He assists McCullen in his evil plans, but secretly has plans of his own. Real name: Rex Lewis

Non-Action Big Bad: Downplayed. While he does leaves the dirty work to his Vipers and McCullen, he does use his tools in wanting to torture Duke. Which makes sense since Rex blames Duke for his apparent death.

Destro

The other half of the Big Bad Duumvirate of the first film, though more of The Heavy. A weapons designer and founder of the Military Armament Research Syndicate (MARS). His terrorist agenda sets up the movie's plot. Real name: James McCullen XXIV. Destro's company manufactures 70% of the world's armaments.

The Baroness

Cobra Intelligence Officer who married a wealthy European aristocrat before she has Storm Shadow kill him/ Real name: Ana Lewis aka Anastascia DeCobray.

Played by: Sienna Miller

Storm Shadow

Member of the ninja clan Arashikage, trained under his uncle, the Hard Master. He is the rival of Snake Eyes. Real name: Thomas Arashikage.

Played by: Byung-hun Lee

Back from the Dead: He returns in the sequel with no explanation how he survived after falling to his apparent death, though was treated at a hidden mountain fortress to be treated for severe burns in the sequel.

Which kind of makes sense because in Japan white is the color of death.

Interchangeable Asian Cultures: Sort of. His last name is Japanese, but his actor's ethnicity (as well as the fact that he spoke Korean as a kid) implies he's actually an adopted Korean.

I Surrender, Suckers: He willingly gets himself caught as Snake Eyes, and even after being revealed to be Storm Shadow, he pretends his heart rate has gone down so that he can get out of the capsule and free Cobra Commander.

Played by: Arnold Vosloo, Jonathan Pryce (when he's pretending to be the President of the United States)

The Bad Guy Wins: By the end of the first movie, he has taken over the presidency of the United States of the America by impersonating the President (and keeping the real President detained). In the second movie, he annihilates most of the G.I. Joes.

Dragon-in-Chief: In Retaliation, Zartan is the President of the United States and puts most of the plot in motion, but he still answers to Cobra Commander.

The Heavy: In Retaliation, while Cobra Commander is the Big Bad, Zartan is the most prominent antagonist in the film and he does more to set Cobra's evil plan in motion.

Karmic Death: He murdered a female GI Joe member in front of Storm Shadow, a man who's disgusted with killing women, and one movie later he gets killed by the same man who he killed the woman in front of because he murdered his master.

Walking Spoiler: It is hard to describe Zartan and how vital he plays to Cobra's plans.

Doctor Mindbender

A scientist in McCullen's employ who developed the nanomite technology and trained "The Doctor" in the field. His current status is unknown. Real name: Unknown.

Played by: Kevin J. O'Connor

Decoy Antagonist: A lot of fans familiar with the mythology expected the Doctor to actually be the Rise of Cobra version of Doctor Mindbender. Only at the end, was it revealed that the Doctor is actually Cobra Commander.

Demoted to Extra: More of an inversion. The character was never intended to have a name, and was only there as a plot device for The Doctor's path to darkness. After the fact, they realized he could easily be Mindbender and threw his name in for the fans.

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