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Determination of copper complexation in seawater using flow injection analysis with chemiluminescence detection,

Description

Flow injection analysis with chemiluminescence detection (FIA-CL) via the reaction of copper with 1,10-phenanthroline was used for the determination of copper complexation in seawater. A detection limit of 0.1nM Cu in undiluted seawater without sample preconcentration was obtained. Copper speciation was assessed by titrating natural seawater ligands with added copper. This method was used to measure copper complexation throughout the water column in Monterey Bay, California. Two ligand classes were identified: a strong ligand (L1), ranging in concentration from 1.3 to 3.1nM, and a weaker ligand (L2), ranging from 9.4 to 25.6nM. The average conditional stability constant for the strong ligand was logK1' 13.1±0.4 and for the weaker ligand logK2' 9.4±0.6. These measurements are in good agreement with complexation studies previously performed using established methods. This technique may eventually be adapted for in situ complexation measurements that can be used to monitor natural spatial and temporal variations in copper complexation, as well as coastal ecosystem responses to urban and industrial change., Cited By (since 1996):33, CODEN: ACACA, ,

Determination of copper in sea water using a flow-injection method with chemiluminescence detection,

Description

Dissolved copper can be rapidly determined in sea water by using a modification of a chemiluminescence method. The determination is based on the formation of a complex between copper and 1,10-phenanthroline and the subsequent emission of light during the oxidation of the complex by hydrogen peroxide. A flow-injection manifold was constructed which provides for the separation of copper from the sea water matrix with a column of immobilized 8-hydroxyquinoline. The column eluant was injected into a carrier stream, mixed with the reagents and delivered to a flow cell where emitted light was detected and used to quantify the amount of copper in the sample. The technique is noteworthy for its low detection limit (0.4 nM), the rapidity of sample processing (approximately 8 min sample -1), the small sample volumes required (approximately 4 ml), and the minimal precautions necessary for the prevention of sample contamination during shipboard processing., Cited By (since 1996):36, CODEN: ACACA, ,