应用

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用

Ab评论

说明

ELISA

1/1000 - 1/5000.

WB

1/1000 - 1/5000. Detects a band of approximately 16 kDa.

靶标

功能May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.

疾病相关Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.

I am sorry ab28955 has not been working for you in western blot. I am sending you a positive control leptin protein free of charge. ab73532 has a 2-3 week wait time, so I sent you ab646 (http://www.abcam.com/lept...

I appreciate your sharing of the protocol with me. The only reason I think it may be worth trying the 20% gel is that your lower percentage gels may cause the protein to run off your gel since the 16 kDa portion of yo...

I am sorry to hear that you have been experiencing issues when using this antibody. This product is covered by our Abpromise guarantee. According to Abpromise guarantee, if this product has been purchased within...