To analyze fluorescence and execute pressure test, hydrogel samples had to be cut into small pieces and smashed up against the glass in order to emulate sheer pressure tests

To simulate sheer pressure testing on the hydrogels, Pasteur pipettes were bent at different angles to determine which formation would provide the most sheer pressure and largest fluorescence signal. (Pipette pictures soon to be uploaded)

The same sample, 90:10 PVOH 146K 110% Laponite w/ DMHXLBR, was used to determine which Pasteur pipette modification proved useful and most result worthy when providing a fluorescence signal.

It appears as if using an unmodified pipette or a pipette with a bend at the top of the pipette so the sides are almost touching produced the maximum amount of sheer pressure.