Received the World Food Day award (FAO/Egyptian Ministry of Agriculture and Land Reclamation), under title “Social Protection and Agriculture: Breaking the Cycle of Rural Poverty”, for my efforts in campaign raise avian influenza awareness in Egypt. December 2015

Molecular diagnosis and characterization of of avian influenza, Friedrich Loeffler Institute, O.I.E and national laboratory for Avian Influenza, Südufer , Germany. In the frame of an OIE twinning project. 2009.

Study the genetic, antigenic and evolution of newly emerged IBDV in Egypt under supervision of Prof. Nicolas Eterradossi the head of OIE reference laboratory of IBDV. Techniques used (real time and conventional PCR – Primer designs – antigen capture ELISA – full genome sequencing of IBDV – in vivo study of the virus pathogenicty - cloning of both virus segments on expression plasmid – virus isolation in tissue culture and in spf egg).

Biotechnology Unit, Reference Laboratory for veterinary quality control of poultry production (RLQP), Animal Health Research Institute:

1- Share as authorized member of Biotechnology unit to follow up the technical and managemental requirements of ISO17025 in biotechnology unit.2- Share in internal audite for other units (2008-2015).3- Since 2009 i share in a team responsible for establishment of 6 satellite-accredited (ISO 17025) laboratories distributed all over Egypt (Luxor, El Fayoum, Sharkia, Ismalia, Gamasa and Alexandria) to test avian viral and bacterial diseases.4- Deputy Head of the research group responsible for identify and characterize of newly emerged avian diseases with special reference to respiratory complex diseases (AIV-H5N1 and H9N2, NDV genotype VII , IB, IBDV and mycoplasmosis) using molecular techniques (real time and conventional PCR).5- Evaluation of vaccines via quantification of pathogens shedding after vaccination and challenge and evaluation of avian immune response via quantification of avian cytokines.Microbiology and immunology department, Misr university (2013-2015):Teaching undergraduates courses of general microbiology and immunology in Misr University. Further supervise postgraduate practical parts of master and PhD thesis in the laboratory.

Influenza and Prion Disease Research Center, National Institute of Animal Health (NIAH), National Agriculture and Food Research Organization (NARO),Tusukuba, Japan (2012-2013):

Doctoral Thesis research conducted under supervision of Prof. Dr. Takehiko Saito, under title “Molecular detection of avian cytokines in response to infection and immunization with Egyptian highly pathogenic avian influenza (H5N1) classical and variant strain” Technique used: (Quantification of Cytokines gene expression using Taq Real-Time PCR and ELISA, gene sequencing , viral isolation and experimental animal infection in Biosafety level 3 laboratory). Training on good laboratory practice (GLP) to be authorized for working in biosafety level 2 as well as biosafety level 3 laboratories.

Biotechnology Unit, RLQP, AHRI, Egypt and Center for Tropical Research Institute of the Environment and Sustainability, University of California, Los Angeles (2009-2012):

I shared as the Egyptian contact person to organize the cooperation with Prof. Thomas B Smith and Dr. Kevin Y Najbo. to investigate the Dynamics of cross-species influenza transmission in 5 governorates in Egypt. Technique used: (sample collection from wild birds, Real Time PCR, viral isolation and gene sequencing).

Using Primary and cell line culture techniques for isolation and characterization of avian viral diseases.

Avian virus isolation in specific pathogen free eggs.

ELISA ,AGPT, HI and HA test.

Using of gene bank tools to characterize the newly emerged pathogens.

Familiar with using of different programs for sequence alignment, curation and then phylogenetic tree analysis as (MEGA6 – Bioedite, MAFFT, DNA star, IQ-TREE) in addition to evolution analysis as (BEAST analysis and Data Monkey)

Using protein structure homology-modelling program as SWISS-MODEL program.

Gene cloning and protein expression.

SDS and immuneblotting techniques.

Follow the recommendations of ISO 17025.

Internal auditor of ISO 17025 for laboratory practicing.

Sharing in the proficiency tests and inter-lab comparison tests come to the lab.

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