DIAKEY NSE ELISA

Intended Use

Enzyme Immunoassay for quantitative determination of NSE Antigen in human serum or plasma

Principle of the Assay

The present method is based upon two anti-NSE monoclonal antibodies recognizing two different epitopes of the molecule. One antibody is adsorbed to the solid phase (coated tube), the other - labeled with Enzyme is used as tracer. The sample to be tested is first incubated in the coated tube. Following this incubation and after an aspirating / washing cycle, the labeled antibody is added to each coated tube, where it is bound to the solid phase by means of the antigen in the standards and (if present) in samples. The amount of binding will be directly proportional to the antigen concentration. After tracer incubation, another washing / aspirating cycle will remove any liquid reagent residue.

Handling Precaution

Do not use mixed reagents from different lots.

Do not use reagents beyond the expiration date.

Use distilled water stored in clean container.

Use an individual disposable tip for each sample and reagent, to prevent the possible cross-contamination among the samples.