Recent reports suggest that sex icon and Playboy editor-in-chief Hugh Hefner and Holly Madison, one of his girlfriends on the reality series The Girls Next Door, have sought fertility treatment in an effort to get pregnant. On the other hand, the couple hasn't given up on having a child naturally.

Madison, who is also considered as Hefner's number one girlfriend, has never made it a secret that she would like to get married to the Playboy founder and eventually have a kid of her own. However, she has never really confirmed their baby plans until now.

While a wedding may not yet be in the cards for Madison and Hefner, Madison reveals that they have been working hard for a tot.

“There has been lots of trying…lots of trying,” Madison told Us Magazine at Playboy's Ninth Annual Super Saturday Night bash in Arizona.

Hefner, on the other hand, has been tight-lipped about his plans to tie the knot for the third time. The 81-year-old sex icon has been married to Mildred Williams from 1949 to 1959, prior to his 10-year marriage to Kimberley Conrad, but considers his relationship with Madison as something that will last for forever.

"I love Holly the most. Five or six years ago, she was simply one of seven girlfriends, but... I fell deeply in love with her. She's the relationship that will last the rest of my life," Hefner said.

“There will certainly be a lot of romantic years ahead, but a wedding we will see,” Hefner told Us Magazine.

Hugh Hefner is down a girlfriend. Hefner and Holly Madison, one of E!'s "The Girls Next Door," are no longer dating. Hefner said he's been "down in the dumps" about the split.

The 28-year-old model-actress stars in the reality series with Kendra Wilkinson and Bridget Marquardt as one of the 82-year-old publisher's girlfriends who live with Hefner in the Playboy Mansion.

"If Holly says it's over, I guess it's over," Hefner said in a telephone interview Wednesday. "She's still here in the house. Until a few days ago, we were still sharing the same bed."

Hefner was surprised to hear Madison discussing their breakup, but acknowledged he knew a split was imminent after he told Madison that they would never wed or have children.

In a video posted on TMZ.com Tuesday, Madison said she's no longer with Hefner. She also said she is "still filming stuff together" with Wilkinson and Marquardt.

Hefner said Madison learned the pair would never have children or get married six months ago, adding: "The fact that she was depressed after that, I didn't know at all. That was a revelation in the last days and weeks. Quite frankly, we thought when the time came, we would make a combined statement and we expected that combined statement would be somewhere in the weeks and months ahead."

"The Girls Next Door" premiered on E! in 2001 and is in its fifth season. Hefner said he and the three women are committed to a sixth season, but he plans to seek out new live-in lovers. Hefner said 19-year-old Playmate twins Karissa and Kristina Shannon are living in the mansion, but they're aren't his girlfriends — yet.

"It's now apparent there will be some new faces in my personal life and on the show," he said. "There's been moments that I've been down in the dumps about all this, and (personal assistant) Mary (O'Connor) told me to cheer up and pointed out that there are girls lined up outside the front gate. At my age, that's hard to believe, but it seems to be true."

Hefner's relationship with his remaining two girlfriends — Marquardt, 35, and Wilkinson, 23 — may also be in flux. Hefner said Marquardt is in Europe filming the new Travel Channel series "Bridget's Beaches," and Wilkinson may soon move out of the mansion and get her own apartment. Hefner said E! is interested in spin-offs with all three women.

The Playboy mogul expects to maintain a business relationship and friendship with Madison, who — along with Marquardt — originally was one of seven girlfriends living with Hefner in 2001 after his separation from Kimberley Conrad. Wilkinson was later asked to move into the Playboy Mansion in 2004. Hefner said he may again seek out seven — or more — girlfriends.

"It's a big house," he said. "And I'm not going to live alone. I'm definitely not going to live alone."

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