BFAR AntibodyCATALOG NUMBER: 5591

Western blot analysis of BFAR in human kidney tissue lysate with BFAR antibody at (A) 1 and (B) 2 μg/mL.

Immunofluorescence of BFAR in mouse kidney tissue with BFAR antibody at 20 μg/mL.

Immunohistochemistry of BFAR in mouse kidney tissue with BFAR antibody at 5 μg/mL.

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0.02 mg - $79.00

0.1 mg - $335.00

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Specifications

Properties

Additional Info

Background

Specifications

SPECIES REACTIVITY:

Human, Mouse, Rat

TESTED APPLICATIONS:

ELISA, IF, IHC-P, WB

APPLICATIONS:

BFAR antibody can be used for detection of BFAR by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

USER NOTE:

Optimal dilutions for each application to be determined by the researcher.

POSITIVE CONTROL:

1) Cat. No. 1305 - Human Kidney Tissue Lysate

IMMUNOGEN:

BFAR antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human BFAR.

The immunogen is located within amino acids 300 - 350 of BFAR.

HOST SPECIES:

Rabbit

Properties

PURIFICATION:

BFAR Antibody is affinity chromatography purified via peptide column.

PHYSICAL STATE:

Liquid

BUFFER:

BFAR Antibody is supplied in PBS containing 0.02% sodium azide.

CONCENTRATION:

1 mg/mL

STORAGE CONDITIONS:

BFAR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

Background

BACKGROUND:

BFAR Antibody: The bifunctional apoptosis inhibitor (BFAR) is scaffold protein that integrates signaling components of the cells apoptosis-regulatory machinery. BFAR is a multidomain protein capable of inhibiting apoptosis induced by TNF-family death receptors ('extrinsic pathway') as well as mitochondria-dependent apoptosis ('intrinsic pathway'). Interaction of BFAR with Bcl-2 or Bcl-XL via a SAM domain may contribute to the anti-apoptotic properties of BFAR. In addition, BFAR contains a DED-like domain that is capable of suppressing apoptosis mediated at the receptor level. BFAR is also thought to be involved in the regulation of neuronal survival.