NTR 366L Spring 2011 Name, eid: Thu Hoang, tah 798 Date: Unique No. Homework 8.1 1. (2 pts) What do the abbreviations cDNA and RT-PCR stand for? (Be sure to spell them out completely and with correct spelling!) cDNA : complementary deoxyribonucleic acid RT-PCR: reverse transcriptase polymerase chain reactionn 2. (4 pts) Very briefly, what is the function of reverse transcriptase in the life cycle of retroviruses like HIV-1? The function is to transcribe the RNA genome of the virus to DNA. Reverse transcriptase

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Unformatted text preview: synthesizes a double stranded DNA copy of the genomic DNA template. 3. (2 pts) In addition to reverse transcriptase and RNA template, what other components must be present for synthesis of cDNA? You need different primers 4. (2 pts) The reaction mixture contains an RNase inhibitor. List at least 2 things that you should do to protect the RNA in the sample from degradation by RNases. Two different ways to protect the RNA in the sample are wearing new gloves and using sterile tubes and pipette tips....
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