'''Figure 3:''' The image above shows the circuit for Module 3 (Degradation). The Constitutive Promoter (BBa_J23119) drives constant expression of the Laccase BioBrick (BBa_K729002). The Laccase gene we intend to use originates from a particular bacterial strain, and is capable of Polyethylene Degradation.

'''Figure 3:''' The image above shows the circuit for Module 3 (Degradation). The Constitutive Promoter (BBa_J23119) drives constant expression of the Laccase BioBrick (BBa_K729002). The Laccase gene we intend to use originates from a particular bacterial strain, and is capable of Polyethylene Degradation.

'''Figure 4:''' The image above depicts the circuit for Module 4 (Buoyancy). The Starvation Promoter (BBa_K118011) drives expression of the Gas Vesicle Cluster (BBa_I750016) when the environmental glucose concentration is low. The Gas Vesicle Cluster selected originates from ''Bacillus Megaterium'', and induces buoyancy in the cell.

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[[File:BOUY.png|center|900px]]

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'''Figure 4:''' The image above depicts the circuit for Module 4 (Buoyancy). The Starvation Promoter (BBa_K118011) drives expression of theT7 RNA polymerase when the environmental glucose concentration is low, then T7 RNA polymerase produced binds the T7 promoter resulting in the expression of GFP. As a future vision we will include the Gas Vesicle Cluster instead of the reporter gene. The Gas Vesicle Cluster selected originates from ''Bacillus Megaterium'', and induces buoyancy in the cell.

For more information on how this circuit functions, please visit our [[Team:University_College_London/Module_4|Buoyancy Description Subpage]]

For more information on how this circuit functions, please visit our [[Team:University_College_London/Module_4|Buoyancy Description Subpage]]

Module 2: Aggregation

Figure 2: The Figure above illustrates our circuit for Module 2 (Aggregation). The pSal promoter BBa_K228004 (modified - see below) which triggers the Aggregation circuit is turned on when bound by activated NahR protein from Module 1. Once the pSal promoter is activated, it drives expression of the curli cluster of genes (BBa_K729003). The individuals genes of the cluster are shown radiating from BBa_K540000.

Modified BBa_K228004: The BBa_K228004 BioBrick must be modified as it combines both the NahR protein and the pSal promoter. We wish to seperate these.

BBa_K729003: This BioBrick is modified from BBa_K540000, which has a cobalt promoter.

Module 3: Plastic Degradation

Figure 3: The image above shows the circuit for Module 3 (Degradation). The Constitutive Promoter (BBa_J23119) drives constant expression of the Laccase BioBrick (BBa_K729002). The Laccase gene we intend to use originates from a particular bacterial strain, and is capable of Polyethylene Degradation.

Module 4: Buoyancy

Figure 4: The image above depicts the circuit for Module 4 (Buoyancy). The Starvation Promoter (BBa_K118011) drives expression of theT7 RNA polymerase when the environmental glucose concentration is low, then T7 RNA polymerase produced binds the T7 promoter resulting in the expression of GFP. As a future vision we will include the Gas Vesicle Cluster instead of the reporter gene. The Gas Vesicle Cluster selected originates from Bacillus Megaterium, and induces buoyancy in the cell.