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Visualization of sequence features with Dasty and STRAP

Christoph Gille (Humboldt University, Berlin, Germany)

Protein sequence features such as post-translational modifications of amino acids, cleavage sites, catalytic site residues and ligand binding sites, are recorded by data curators from all over the world and stored in public databases. The collections of sequence features are accessible via DAS servers. Here we present two sequence feature viewers - Dasty and STRAP. They are complementary in several aspects, therefore, they can be used in concert. Both have in common that they load sequence features from any registered DAS server and display the up-to-data information along the amino acid sequence. Dasty gives a clear and compact overview of sequence features of one single amino acid sequence. An outstanding feature is the ability to re-arrange the information using the mouse. To compare sequence features of two homologous proteins or to display sequence features of one protein in the context of a sequence alignment or 3D-structure the user can activate the "STRAP"-button in the Dasty view. In the multiple sequence alignment panel of STRAP, the residues with sequence features are underlined. Textural information is not directly shown due to the limited space, but pops up as a balloo