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explaining, "I read somewhere that the human brain is controlled by electrical impulses and how thoughts are stored electronically.I started toying with the story potential of a process that would allow the recording of brain patterns and transferring them to another brain.Sam Loover, a family friend and an agent of World Intelligence Network (WIN), persuades Mac to dedicate the services of Joe and the BIG RAT to the organisation: Joe will become a WIN operative with a difference, the unlimited possibilities offered by the BIG RAT serving as an invaluable tool for completing missions.After requisite knowledge and experience has been transferred, and provided that Joe is wearing customised glasses containing hidden electrodes (a portable storage device for brain impulses), he is able to carry out missions requiring proficiency in – among other disciplines – flying fighter aircraft, Reporting to Shane Weston, the commander-in-chief of WIN's London Headquarters, Joe is also equipped with a special briefcase, which externally appears to be nothing more than a school case but which secretly contains an adapted handgun and transceiver.Created by Gerry and Sylvia Anderson and filmed by Century 21 Productions, the 30-episode series followed Thunderbirds and Captain Scarlet and the Mysterons.First broadcast in the UK between September 1968 on the ATV network, Joe 90 was the sixth and final of the Andersons' productions to be made exclusively using the form of marionette puppetry termed "Supermarionation".The Secret Service, the Andersons' next production after Joe 90, developed the hybridity further with the incorporation of extensive footage of live actors in long shot, intercut with scale puppet sequences.

The script for the pilot (titled only in production documentation as "The Most Special Agent") was written by Anderson and his wife, Sylvia, as was the custom for every new puppet series that the couple developed in the 1960s.

Since he was occupied by post-production on the second Thunderbirds feature film, Thunderbird 6, and the development of his live-action film, Doppelgänger, Gerry Anderson was unable to fulfil the producer role as he had done for Captain Scarlet, and instead passed the responsibility to Reg Hill and David Lane.

Other directors for Joe 90 included Leo Eaton, Alan Perry and Ken Turner, all of whom had contributed to Captain Scarlet, and Peter Anderson, who was promoted from his earlier position as assistant director to replace the outgoing Brian Burgess and Robert Lynn.

An example of the advanced technology demonstrated is Professor Mc Claine's "Jet Air Car": a multiple-configuration land-, sea- and air-based vehicle built prior to the events of the series.

The in-joke of "WIN", the abbreviated form of "World Intelligence Network", is similar to that of "WASP", the acronym for the World Aquanaut Security Patrol that appears in Stingray.