"The constriction of the entering tachyzoite can be localized by immunostaining the surface protein P30, which under nonpermeabilizing conditions sharply demarcates the extra- and intracellular portions of the zoite (Figures 2A–2D, S1A, and S1B), as well as RON4 (Figures 2E, 2F, and S1C), a tachyzoite-secreted protein that redistributes to the MJ ([Lebrun et al., 2005] and [Alexander et al., 2005])."

"Immunofluorescence assay revealed a membrane-associated labeling of this protein (Fig. 6) which was punctuated and not continuously distributed along the plasma membrane compared to uniform labeling with anti-SAG1 (Fig. 6C and D)."

"The extracellular portion of the tachyzoites was labelled with anti-SAG1 (green), and, then, after permeabilization of the cells with saponin, the ring of the MJ was revealed by addition of the conjugate (red)."

"The ALP1 protein was expressed in both the tachyzoite and bradyzoite stages of Toxoplasma. Intracellular tachyzoites were stained for ALP1 using rabbit anti-ALP1 followed by secondary antibodies conjugated to Alexa 488 (green) and the stage-specific surface marker SAG1, detected using mouse mAb DG52, and secondary antibody conjugated to Alexa 594 (red)."

"To determine the percentage of transfected parasites that were adherent, the proportion of c-Myc-positive parasites in the starting inoculum (c-Myc positive) was compared with the proportion of parasites that were attached to host cells following a brief invasion pulse and staining for the surface protein SAG1 (c-Myc positive and SAG positive)."

"The major surface antigen (SAG1) is detected prior to permeabilization to label the extracellular portion of the penetrating parasites preferentially, while TgMLC1 was visualized after permeabilization"

"SAG1TM-CDMIC6 accumulates precisely in the micronemes as demonstrated by immunoelectron microscopy using anti-SAG1 antibodies. SAG1 is detected by gold particles and, when GPI anchored, was found in its normal location at the parasite surface (arrowheads)."

parasite plasma membrane during after tachyzoite invasion and extracellular tachyzoite, vesicle budding off zoite posterior during after tachyzoite invasion

"immunoperoxidase detection of the major surface proteins SAG1 and SAG2 revealed that they were distributed uniformly on the surface of extracellular tachyzoites. During invasion of the host cell the majority of the surface label failed to pass throught the tight junction. The label appeared to remain associated with membrane accumulating at the posterior of the zoite, where it was shed in the form of vesicles. This pattern of redistribution was observed with both surface molecules (Figs. 1, 4). However, in some in- stances, shedding of the surface coat was not complete and varying amounts of label were internalised and re- mained on membrane associated with or adjacent to the parasite surface "

Microscopy type: EM

Microscopy method: Monoclonal antibody directly to protein

Strain: RH

Gene model mapping comments: inferred from another publication

Localisation record: surface during extracellular tachyzoite, some parasite plasma membrane during after tachyzoite invasion, vesicles budding off zoite posterior during after tachyzoite invasion