Re: [Histonet] IHC screening on TMA slides

From:

"Jackie M O'Connor"

I don't understand why you would have a TMA with 100 cores of the same
sample.
Ana MERINO-TRIGO
Sent by: histonet-bounces@lists.utsouthwestern.edu
03/02/2007 11:57 AM
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[Histonet] IHC screening on TMA slides
Hi Histonet,
I was wondering if anyone could give me any ideas or suggestions in order
to perform IHC screening over a TMA slide containing about 100 spots of
0.6 or 1.0 mm of the same tissue. The idea it will be to select hybridomes
for further characterization based on IHC reactivity on a given positive
control tissue. Around 100 hybridomas to test on a TMA slide containing
spots from the same sample. I cannot see the way to do the immuno without
spread the antibody in between the spots. I heard that exits multispot
type of lids that you could put over the slide to perform the immno but I
don't really know where to look for these lids or if they are good enough
to be sure that antibody doesn't spread in between spots.
Any information it will be really appreciate it,
All the best,
Ana
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