Bovine Genotyping—Thermo Scientific

We offer three reagent kits for cattle parentage testing and identification using microsatellite (STR) loci. The Thermo Scientific Bovine Genotyping kits allow co-amplification of the microsatellites in a single multiplex PCR reaction. The reagents and protocols of the kits have been optimized to deliver similar peak sizes (amplification yields) for each loci. The kits utilize Phusion Hot Start DNA Polymerase.

Allele callings obtained with the kits represent the true alleles of an individual, instead of 'plus-A peaks' or 'split peaks' typically interpreted when using e.g. a conventional Taq DNA polymerase. This is due to the proofreading activity of the Phusion DNA Polymerase.

Thermo Scientific Bovine Genotypes Panel 1.2 encompasses all the 12 STR loci recommended by the International Society for Animal Genetics (ISAG) for routine use in bovine parentage testing and identification, including:

Thermo Scientific Bovine Genotypes Panel 2.2 encompasses the following 6 loci recommended by the International Society for Animal Genetics (ISAG) and the Food and Agriculture Organization of the United Nations (FAO) for genetic studies of domestic animals, including:

Thermo Scientific Bovine Genotypes Panel 3.1 encompasses all the 12 STR loci from panel 1.2 recommended by the International Society for Animal Genetics (ISAG) for routine use in parentage testing and identification and the 6 STR loci from panel 2.2 recommended by the Food and Agriculture Organization of the United Nations (FAO) for genetic studies of domestic animals.

Thermo Scientific Bovine Genotypes Panel 3.1 is a combination of Panels 1.2 and 2.2, thus enabling the analysis of both panels in a single reaction.