Abstract: The response of growth hormone to acute exercise has been shown to be altered by training. The present study examined the time course of GH adaptation to 3-weeks of sprint exercise training on a cycle ergometer. Eight, recreationally active, male subjects (age = 26.8 ± 4.3 yrs; height = 1.77 ± 0.05 m; weight = 77.79 ± 7.35 kg; lean body mass = 62.0 ± 7.1 kg) completed a 2-hour resting profile and a 2-hr acute sprint profile, followed by three weeks of training with additional acute sprint tests at the end of each week. Blood samples were taken every 15 minutes [Q15] during rest and more frequently [Q1-Q10] during exercise. Body composition was assessed by DXA [Prodigy Advanced, GE Lunar]. Training consisted of 4-6 repetitions of 30-second maximal sprints relative to body mass, three times per week. Integrated 2 hr serum GH area under the curve (AUC) was calculated using trapezoidal integration. Peak power (PP) increased 7.5% while time to peak power (TTP) decreased 58.0 % (P < 0.05) over the 3-week training period. The mean of 2 hr GH AUC per unit lean body mass (LBM), did not significantly decrease (P = 0.82) during the 3-week sprint training protocol, although a trend was observed for GH AUC to decrease from the first acute sprint test to the second acute sprint test (8.45 ± 3.0 ng/ml vs. 3.59 ± 1.21 ng/ml, respectively; P = 0.90). While this trend was similar to previously reported findings, we conclude that 3-weeks of sprint training does not significantly alter the GH AUC response to acute sprint exercise despite significant increases in power output during this same time frame.