Chemical structure

Related Biological Data

RPE cells were treated with TSA at various concentrations (0.2, 0.4, 0.8 and 1.0 μM). The protein expression levels of cyclinD1, CDK4 and CDK6, p-Rb, P21 and P27 were detected by western blot.

Related Biological Data

TSA replaces FGF-2 in the reprogramming process

Related Biological Data

Biological Activity

Targets

HDAC

IC50

~1.8 nM

Protocol

Cell experiment[1]:

Cell lines

Human breast cancer cell line

Preparation method

The solubility of this compound in DMSO is <10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while.Stock solution can be stored below -20°C for several months.

Background

Trichostatin A (TSA) is a potent inhibitor of histone deacetylase (HDAC) as well as an antifungal antibiotic with cytostatic and differentiating properties that noncompetivively and reversibly inhibits HDAC, at low nanomolar concentrations, in both cultured mammalian cells and fractionated cell nuclear extracts. It is capable of arresting cells in G1 and G2 phases of the cell cycle, inducing differentiation and reverting the transformed morphology of cells in culture. According to a study investigating the effect of TSA in human breast cancer cell lines, TSA inhibited proliferation of breast carcinoma cell lines (IC50 124.4 ± 120.4 nM), comparing to all cell lines (IC50 2.4 ± 0.5 nM), and resulted in pronounced histone H4 hyperacetylation.