Abstract: A high performance liquid chromatography with evaporative light-scatter detector （HPLC-ELSD）quantitativemethod for monoglyceryl ester was established. The monoglyceryl ester was directly dissolved by isopropyl alcohol，andfiltered by 0.22μm microfiltration membrane. Using C18 reverse phase chromatography column，acetonitrile and isopropylalcohol were used as mobile phase，to gradient elution，with a flow rate of 0.5mL/min. The drift tube temperature wascontrolled at 45℃ and atomizer temperature at 36℃. The pressure of carrier gas was controlled at 25psi. Experimental resultsshowed that the components can be separated completely among monoglyceride palmitate，monoglyceric stearate，1，3-Dipalmitin，1，2-Dipalmitin，1，2-Distearin，1，3-Distearin. The common logarithm for peak area and concentrationshowed good linear relationship（R2 >0.996）. The limit of detection（S/N=3）of the method was 0.01～0.02μg and the limitof quantitation（S/N=10）was 0.03～0.04μg. The average standardized recycling ranged from 94.62% to 103.70%and therelative standard deviation （RSD）was less than 5%. The method is simple and rapid for quantifying monoester anddiglyceride contents in monoglyceryl ester without derivatization.