QIAamp One-For-All Nucleic Acid Kit

For automated purification of viral DNA and RNA or genomic DNA from a variety of sample types

Rapid isolation of high-quality, viral DNA and RNA or genomic DNA

No organic extraction or alcohol precipitation

Consistent, high yields

Complete removal of contaminants and inhibitors

Flexibility with multiple sample types in the same run

The QIAamp One-For-All Nucleic Acid Kit provides automated purification of viral nucleic acids or genomic DNA on the BioRobot MDx or the BioRobot Universal System using proven QIAamp technology. The fully automated procedure requires less than 2.5 hours, including bar code reading and complete process documentation, with no hands-on time during the run. The QIAamp One-For-All Nucleic Acid Kit enables processing of different samples (e.g., viral RNA and DNA from serum, genomic DNA from blood) in the same run.

Viral DNA was purified from serial dilutions (2000-50 copies/ml) of CMV-positive sample QCMD 05-07 using the QIAamp One-For-All Nucleic Acid Kit on the BioRobot MDx. [A] Viral DNA was analyzed by quantitative real-time PCR. The limit of detection was lower than the lowest dilution (<50 copies/ml). [B]Internal control signals for all samples demonstrate efficient DNA recovery for all samples.

Consistent, high yields

The kit provides consistent high yields of viral nucleic acids over a wide range of viral loads (see figure "Reliable purification of viral DNA"). Automation ensures a high degree of standardization for consistent, repeatable results (see figure "Repeatable purification of viral nucleic acids"). DNA and RNA purified using the QIAamp One-For-All Nucleic Acid Kit can be used in a range of downstream applications, including:

PCR and quantitative real-time PCR and RT-PCR

Infectious disease research

Principle

The QIAamp One-For-All Nucleic Acid Kit simplifies isolation of viral RNA and DNA from a wide range of sample types with a fast vacuum procedure. No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in three efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit.

QIAamp sample preparation technology is fully licensed.

Procedure

Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective nucleic acid adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp 96-well plate. Wash buffers are used to remove impurities. Viral nucleic acids, genomic DNA, and cellular RNA are eluted, and ready for use in amplification reactions or storage at –20ºC. Purified nucleic acids are free of proteins, nucleases, and other impurities.

Applications

The QIAamp One-For-All Nucleic Acid Kit is designed to allow automated processing of multiple sample types in the same run. Sample types include: