Protein Summary

Pfam note: this protein is now in family PF07366. 5 SnoaL in CL0051, with other structures.

Gene BTH_II0226 from Burkholderia thailandensis encodes the YP_438428.1 protein that belongs to the SnoaL-like polyketide cyclase family (PF07366). The BLAST search results indicate that this protein is a member of the NTF2-like superfamily.

Pre-SCOP classifies 3h3h in the alpha+beta class, NTF2-like superfamily, ketosteroid isomerase-like (KIS) family. DALI, FFAS and SSM suggest that 3h3h is a structurally similar to ketosteroid isomerases and steroid delta-isomerases like

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(Z=16). However, the active site is very different to these structures. The ketosteroid isomerases have an active site formed by residues Tyr16, Tyr32, Asp40, Tyr57, and Asp103 (numbering from wild type KIS PDB entry

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) which corresponds to residues Trp16, Tyr32, Ser40, Trp64, and Glu102. In fact, residues Trp16, Trp20, Tyr32, Met42, Trp64, Tyr92, Glu102, Tyr104 and Tyr119 sketch a very hydrophobic active site cavity in each subunit with unassigned electron density. One unknown ligand is modeled in each putative active site. The interface interaction indicates that the biomolecule of protein YP_438428.1 could be a dimer.