Recombinant Mouse Interleukin-7 produced inE.Coliis a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 14897 Dalton. The IL-7 is purified by proprietary chromatographic techniques.

Description:

Interleukin-7 is a potent lymphoid cell growth factor produced primarily by stromal cells. IL-7 has been shown to support the proliferation and differentiation of pre B- and early T cells as well as displaying a biological effect on cells of NK and myeloid lineages.

Source:

Escherichia Coli.

Amino Acid Sequence:

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Cys-His-Ile.

Lyophilized from a concentrated (1 mg/ml) solution in water containing no additives.

Solubility:

It is recommended to reconstitute the lyophilized Interleukin 7 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Specific Activity:

The ED50as determined by the dose-dependant stimulation of murine 2E8 cells is < 0.2 ng/ml, corresponding to a Specific Activity of 2 x 106 IU/mg.

Protein Content:

Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.48 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-7 as a Reference Standard.

Stability:

Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL7 should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.