-ranging from age 17 to 60-must be at least 45kg

-be in good general health with no history of hepatitis or HIV/AIDS-at least 6 hours of sleep on the day before-free from chronic, infectious diseases

-previous blood donation must be more than 3 months ago-have not gone through major operation for the past 1 year-have not gotten malaria for the past 3 years-have not practiced unsafe sex-have not shared the same needles with others

-have not consumed alcohol a day before-have never tested positive for HIV-have not had sex with the partners of the same sex even once.-do not have hemophilia or another Blood clotting disorder and received clottingfactor concentrate.-have not been held in a correctional facility (including jails, prisons and/or detention centers) for more than 72 hours in the last 12 months.