is it possible to do a WB from cells that were fixed before (Cytoperm/Cytofix, contains PFA)? I am currently looking at literature and searching the internet on this topic, but I still wanted to ask you bioforumers for your opinion/advice/experience !

Thanks for the reply, doxorubicin !
Hm, with immunostaining it's not really quantitative, I suppose... and RNAScope is rather for tissue sections I think ? Had been considering ELISA as an alternative though.