[1]陈雪丽,李伟群,王英,等.黑土区连作大豆根际土壤氨氧化古菌群落结构特征及其驱动因子分析[J].大豆科学,2016,35(04):620-625.[doi:10.11861/j.issn.1000-9841.2016.04.0620] CHEN Xue-li,LI Wei-qun,WANG Ying,et al.Characterization of Ammonia Oxidizing Archaea Community and Its DrivingFactors in the Ｒhizosphere of Continuous Cropping Soybean in Black Soil[J].Soybean Science,2016,35(04):620-625.[doi:10.11861/j.issn.1000-9841.2016.04.0620]

Continuous cropping resulted in the changing of metabolism process in the rhizosphere of soybean，while the communitycomposition of nitrogen related functional genes were always used to evaluate the response of the nitrogen conversion capabilityof soil to continuous cropping system. Ammonia oxidizing was the first step in the course of nitrogen transformation andalso the limited speed step，so that ammonia oxidizing bacteria( AOB) and ammonia oxidizing archaea were always consideredthe driver of the ni trogen transformation process. In this study，the methods of qPCＲ and Illumina Miseq sequencing were usedto analyze the ammonia oxidizing archaea( AOA) community composition and quantities in rhizosphere of soybean based on thesoybean continuous cropping located observation in black soil. The results showed that the abundance of Archaea amoA gene inSC2 and SC17 were lower than that in other treatments. AOA in the rhizosphere of soybean belongs to two phylums，Crenarchaeotaand Thaumarchaeota，and still have unclassified AOA accounted for 42%. The correlated analysis between AOA compositionand environment factors indicated that the AOA compositions of MS ( maize-soybean rotation) and SC2 were positiverelated to the contents of total nitrogen and total carbon，then that of treatments with more than continuous cropping years of 4showed positive related to pH and C/N ratio.