Phospho-VEGF Receptor 2 (Tyr951) Antibody (PA5-36700)

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Product Details

Bioinformatics

Documents

Synthetic phosphopeptide derived from human VEGFR2 around the phosphorylation site of Tyrosine 951

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/ml

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.05% sodium azide

Storage Conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Tested Applications

Dilution *

Immunofluorescence (IF)

1:50-1:200

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Western Blot (WB)

1:500-1:1000

* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the
product for use in their own experiment using appropriate negative and positive controls.

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 150 and 210 kDa.

Purity is >95% by SDS-PAGE.

Background/Target Information

Three cell membrane receptor tyrosine kinases, Flt (also designated VEGF-R1), Flk-1 (also designated VEGF-R2) and Flt-4, putatively involved in the growth of endothelial cells, are characterized by the presence of seven immunoglobulinlike sequences in their extracellular domain. These receptors exhibit high degrees of sequence relatedness to each other as well as lesser degrees of relatedness to the class III receptors including CSF-1/Fms, PDGR, SLFR/Kit and Flt-3/Flk-2. Two members of this receptor class, Flt-1 and Flk-1, have been shown to represent high affinity receptors for vascular endothelial growth factors (VEGFs). On the basis of structural similarity to Flt and Flk-1, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.