Services

With our expertise, ZellNet strives to make your Elispot experience successful, outstanding, and enjoyable. Our unsurpassed customer service is ready to assist you.

We offer help

Starting with the setup of experiments to the final evaluation of plates and statistical analysis of results, from experimental design of research projects to establishing qualification and validation plans for Elispot – ZellNet provides help when and where you need it.

On-site service

We are able to come to your lab to conduct a wet workshop following your experimental requirements and environment to get you started like a Pro. Looking to outsource your assay, maybe also sample handling, preparation and storage? We can help you in your task.

Plate reading service

Don’t want to buy an expensive Elispot reader system? Would you prefer to have your samples evaluated in a blinded fashion, removing all the hassles of potential bias as well as of establishing a qualified plate reading SOP in your lab? No time for efficient training, updates and upgrades? ZellNet specializes in superior plate reading services for any Elispot and Fluorospot assay and plate format since 1998.

Elispot Plate Reading Service including dual color, fluorescent and 384 well plates. Plates will be evaluated in high resolution using automated Elispot reader systems from Zeiss (Elispot) and AID (Fluorospot). The following information will be provided:

number of spots per well

size of each spot in each well

intensity of each spot in each well

total area per well covered by spots

plate overview image

binary spot picture of all wells

single well images

reader settings

Well images can be used for publication, demonstration and presentation purposes. Per request, we can also provide high resolution well images for re-evaluation purposes.

<20 plates: $ 20.00/month

21 to <50 plates: $50.00/month

>50 plates: $100.00/month

Membrane Removal Service

For highest accuracy, we recommend to remove the membranes from old format Elispot plates (e.g., MAIP or MAHA plates) and transfer them onto sealing tape. This assures that membranes are completely flat and therefore perfectly focused during evaluation (The older plates give membranes room to expand. This leads to concave membranes, with their center being elevated in comparison to the well periphery.).

We analyze your Elispot data for positivity. If more than one antigen are used, the analysis is done with a permutation-based method that takes into account that normal distribution of Elispot data cannot be assumed under commonly used setup conditions of the assay . The method has been shown to control the false positive rate while having superb sensitivity. For positivity testing of results from assays testing just one antigen, the appropriate tests are applied.

Operator-dependent variability of Elispot reading results is a known phenomenon, and has been described.

ZellNet offers a service to certify proficiency in Elispot plate evaluation. Such certification should be periodically pursued for each scientist in a laboratory running its own plate evaluation.

This service is independent of the Elispot reader used in-house. Simply send your plate(s) in for re-evaluation. Specify the request for certification. Also, provide your reading results and, if feasible, setup information of your plate(s), if you wish to obtain statistical analysis of the comparison of both reading results.

We will perform an extensive evaluation of the plate including auditing of all wells, spot appearance, analysis of artifacts and any problems observed (if necessary) and provide a detailed report.