Objective To study the molecular network regulation mechanism of Zuojin Pills against Helicobacter pylori (Hp) infection based on network pharmacology and bioinformatics. Methods By searching all the components of Zuojin Pills in TCMSP, the possible active components and targets were screened and predicted . The targets related to Hp infection were selected in 5 major disease databases, and then the interaction network between component targets and disease targets was constructed to obtain the characteristic gene of Zuojin Pills against Hp infection . GO functional enrichment and KEGG pathway enrichment analysis were performed on the above characteristic genes through DAVID 6.8 database . Cytohubba was used to screen the key anti-Hp targets of Zuojin Pills. Results Totally 32 active components and 197 action targets of Zuojin Pills were screened and predicted by TCMSP. A total of 199 enrichment results were obtained by GO analysis, including 152 biological process of inflammatory response, RNA signal transcription and signal transduction, 19 cellular components of nucleus, extracellular matrix and protein complexes, and 28 molecular function of cytokine activity, DNA binding and ATP binding. KEGG analysis showed that 65 signaling pathways, such as Jak-STAT signaling pathway, T cell receptor signaling pathway, cell cycle signaling pathway and Wnt signaling pathway, were closely related to anti-Hp infection of Zuojin Pills. 10 key genes such as CXCL8, IL10, IL4, VEGF A and MMP9 were screened by Cytohubba. Conclusion Zuojin Pills have the characteristics of multi-components, multi-targets, and integral regulation for the treatment of Hp infection, which can provide basis for the study of active components and efficacy and mechanism of anti-Hp infection.

参考文献/References:

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