Step 7: Verify the clone

SeqBuilder Pro 15.1 and later offers the ability to verify whether or not a clone produced in the laboratory matches the version designed in SeqBuilder Pro. To learn how, watch this brief video or follow the written instructions below:

To verify a clone:

Use SeqBuilder Pro to design a virtual clone. After completing Step 5: Run the cloning simulation, you will be asked if you wish to verify the clone. If you have already created and sequenced the clone in the laboratory, skip to step 6. Most users, however, will continue to step 2.

Save the cloning project.

Create the clone “in real life” in the laboratory.

Sequence the lab clone via Sanger sequencing and put the resulting .abi files on the same computer used to design the virtual clone.

Launch SeqBuilder Pro and use File > Open to open the original cloning project.