Suitable for use in Immunofluorescence microscopy, Immunohistochemistry, Immunoprecipitation, Neutralization Assay, Affinity chromatography and ELISA. Not suitable for use in Immunoblot. Other applications not tested.

Recommended Dilution

Immunofluorescence: 1:20

Immunoprecipitation: 1:5

Optimal dilutions to be determined by the researcher.

Storage and Stability

Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recognizes AAV-2 found in human and monkey in ELISA. Also applicable to AAV-3. Specifically reacts wtih intact AAV particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids not present in denatured capsid proteins and native but unassembled capsid proteins. Mjor reaction attributed to aa369-378 of AAV-2 capsids.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.