4) Add 100mL Mineral Oil (CVS, 99.9%) to a homogenizer and subsequently add the PVOH mixture. Homogenize for ~5minutes to form a w/o emulsion

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5) Pour final w/o emulsion into a beaker and place in a freezer at -20°C for 20 hours. Remove and allow thawing for 4 hours. Repeat this cycle for a total of three cycles.

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==Calculations==

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'''Preparation of 20mM phosphate buffer, pH = 7.4'''

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[Base] = monosodium phosphate, 119.98 g/mol

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[Acid] = disodium phosphate, 141.96 g/mol

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pH = pKa + log ([Base]/[Acid])

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7.4 = 7.3 + log ([Base]/[Acid])

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([Base]/[Acid]) = 1.2589

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[Acid] + [Base] = 20mM

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[Base] = 20mM - [Acid]

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(20mM - [Acid])/[Acid] = 1.2589

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[disodium phosphate] = 8.85mM

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[monosodium phosphate] = 11.14mM

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8.85mmol x 141.96g/mol x 1mol/1000mmol = 1.256g disodium phosphate

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11.14mmol x 119.98g/mol x 1mol/1000mmol = 1.337g monosodium phosphate

==References==

==References==

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This area is for any observations or conclusions that you would like to note.

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http://www.sciencedirect.com/science/article/pii/S0168365998000893

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Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.