Goal

The idea of the "BactoSketch" (new names are welcome) is that you can wave a "pen" over a lawn or film of bacteria, and those cells that have been stimulated would respond by producing a detectable signal (e.g. luminescence or fluorescence). The cells would remember their state until they receive a second signal that erases the pad.

Extras could include multiple output colors or gradation of expression.

LacIts

Collins CI-LacIts Switch

What we got from Collins
Collins has FedEx'ed us on 14 June 2005 the 6 plasmids that we requested (on 13 June 2005). The plasmids were pCIRa, pCIRb, pCIE, pTSMa, pTSMb1, and pTSMb2 from Kobayashi et al., 2004 (PNAS 2004). They arrived on 15 June 2005, but we had to order antibiotics which arrived on 16 June 2005.

Collins also emailed us with the sequences for pTSMb1 and pCIE as well as an MS Excel file with construct descriptions: Collins Constructs.