I am interested in being able to extract DNA from dried blood spots
(Guthrie cards) for PCR and allele specific oligonucleotide analysis
using the Amersham ECL kit. We have found that using simple
extraction methods and amplification we get nonspecific binding of the
labelled probe, which we suspect is due to something else coeluting
from the Guthrie card.
Has anyone used the Amersham kit with amplified DNA from blood
spots?? Cheers.
John Christodoulou
Dept of Paediatrics & Child Health
University of Sydney
Sydney