Although the investigation seems focused on the idea that the Senate powder
could have been homemade, some experts say thats improbable

Anthrax Powder: State of the Art?

GARY MATSUMOTO

Gary Matsumoto, an investigative journalist in New York City, is writing
a book on biodefense.

When the anthrax mailers penned the message, YOU CAN NOT STOP US. WE
HAVE THIS ANTHRAX, the threat included a chilling nuance that remains
largely unrecognized. ARE YOU AFRAID? asked the attackers.
Yes, should have been the answer, according to some biodefense
experts, who think that the anthrax spores mailed to Senators Thomas Daschle
(DSD) and Patrick Leahy (DVT) in the fall of 2001 represented
the state of the art in bioweapons refinement, revealing telltale clues about
the source. This view is controversial, however, because others dispute the
sophistication of the Senate powder, and a schism now exists among scientists
who analyzed it for the FBI.

One group, comprised mostly of microbiologists and molecular biologists,
argues that this material could have been a do-ityourself job, made by someone
knowledgeable but with run-of-the-mill lab equipment on a modest budget.
This contingent includes one well-known bioweaponeer, Ken Alibek, who defected
from Russia to the United States in 1992.

The other faction thinks that the powder mailed to the Senate (widely reported
to be more refined than the one mailed to the TV networks in New York) was
a diabolical advance in biological weapons technology. This diverse group
includes scientists who specialize in biodefense for the Pentagon and other
federal agencies, private-sector scientists who make small particles for
use in pharmaceutical powders, and an electronics researcher, chemist Stuart
Jacobsen of Texas.

Early in the investigation, the FBI appeared to endorse the latter view:
that only a sophisticated lab could have produced the material used in the
Senate attack. This was the consensus among biodefense specialists working
for the government and the military. In May 2002, 16 of these scientists
and physicians published a paper in the Journal of the American Medical
Association, describing the Senate anthrax powder as
weapons-grade and exceptional: high spore concentration,
uniform particle size, low electrostatic charge, treated to reduce
clumping (JAMA, 1 May 2002, p. 2237). Donald A. Henderson, former
assistant secretary for the Office of Public Health Preparedness at the
Department of Health and Human Services, expressed an almost grudging respect:
It just didnt have to be that good to be lethal, he told
Science.

As the investigation dragged on, however, its focus shifted. In a key disclosure,
U.S. Attorney General John Ashcroft revealed in August 2002 that Justice
Department officials had fixed on one of 30 so-called persons of
interest: Steven J. Hatfill, a doctor and virologist who in 1997 conducted
research with the Ebola virus at the U.S. Army Medical Research Institute
of Infectious Diseases in Fort Detrick, Maryland. (Hatfill has denied any
involvement in the anthrax mailing.) Although the FBI did not spell out its
theory, this announcement and leaks to the media from federal investigators
indicated that the inquiry had embraced the idea that a lone operator or
small group with limited resources could have produced the Senate anthrax
powder.

This premise now appears to have run its course. In September 2003, the
FBIs Michael Mason admitted that the bureau failed to reverse engineer
a world-class anthrax powder like the Senate material and expressed regret
that Hatf ill had been called a person of interest. One of the
costliest manhunts ever conducted by federal investigators appears to be
stymied. The FBI cannot or will not say whether the anthrax powder was foreign
or domestic, expensively made or cheaply done, a professional job or the
handiwork of an amateur.

But the scientific data amassed so far should provide a wealth of information
on the weapons possible origins, say scientists in the group with expertise
in such powders. They argue that the most striking qualities of the Senate
powder do not concern the anthrax spores but the way they were
processedspecifically, how they were given an electrostatic charge
and unusual surface properties.

If the Senate anthrax powder did in fact have these refinements, its manufacture
required a unique combination of factors: a strain that originated in the
United States, arcane knowledge, and specialized facilities for production
and containment. And this raises the discomforting possibility that the powder
was made in America, perhaps with the resources of the U.S. government.

Charged questions

There is no debating that the Senate powder was exceptionally pure and highly
concentrated. Nor is there any doubt that it contained the Ames strain, one
of the most virulent strains discovered. But what made it truly remarkable,
according to biodefense specialists, was its conversion into a cutting-edge
aerosol.

That transformation had as much to do with chemistry and physics as with
microbiology. Anthrax spores cling to one another if they get too close;
sticky chains of proteins and sugar molecules on their surfaces latch onto
each other, drawn by van der Waals forces that operate at a distance of a
few tens of angstroms. Untreated spores clump into larger particles that
are too heavy to stay airborne or reach the narrowest passages in the lung.

To thwart this clumping, an earlier generation of biological weapons
makersoperating out of Fort Detrick when it still made
weaponsexperimented with ways to prevent the surfaces of germs from
getting too close. For example, William C. Patrick III, former chief of Fort
Detricks Product Development Division, pioneered the use of a dusty
silica powder with nanometer-sized particles added to nonlethal incapacitating
agents such as Francisella tularensis, the cause of tularemia (but
not Bacillus anthracis, the cause of anthrax). Otherwise,
says Patrick, the powder was very hard to disseminate.

In a separate research arena, pharmaceutical scientists in the 1990s began
experimenting with adding electrostatic charges to small particles in medicinal
powders designed for inhalation. Adding a like charge of sufficient strength
creates an electrostatic field of up to a few centimeters, which makes particles
repel one another, creating an energetic or self-dispersing powder.

Biodefense scientists say they became aware that such an innovation could
be applied to germ-warfare powders with deadly effect, especially deadly
because charged particles are more prone to lodge in the lung. Once in the
lung, immune cells transport the spores to lymph nodes, where the spores
germinate and cause infection. The Senate anthrax spores carried like electrical
charges, and some experts believe that they were added deliberately to aid
dispersal.

Was it a coincidence that this lethal innovation appeared in the anthrax
spores sent to the Senate? Alibek thinks it is possible. The Senate anthrax
could have acquired a charge from friction as the envelopes passed through
mail-sorting machines. (Alibek also has speculated that the powders mailed
to the Senate were more refined than those sent to the New York media and
may have come from a different production run.) But his theory raises a question:
Why would only the Senate powder acquire a charge from the sorting machines?

Jacobsen, a research chemist who coated sub5-micrometer particles with
silica while working on a program for the Defense Advanced Research Projects
Agency (DARPA), is skeptical of this idea. Jacobsen says that friction would
add static electricity only to surfaces: If anything, the sorting
machines pinch rollers and the envelopes should get charged,
he says, not the spores inside.

Glassy finish

More revealing than the electrostatic charge, some experts say, was a technique
used to anchor silica nanoparticles to the surface of spores. About a year
and a half ago, a laboratory analyzing the Senate anthrax spores for the
FBI reported the discovery of what appeared to be a chemical additive that
improved the bond between the silica and the spores. U.S. intelligence officers
informed foreign biodefense off icials that this additive was polymerized
glass. The officials who received this briefingbiowarfare specialists
who work for the governments of two NATO countriessaid they had never
heard of polymerized glass before. This was not surprising. Coupling
agents such as polymerized glass are not part of the usual tool kit
of scientists and engineers making powders designed for human inhalation.
Also known as sol gel or spin-on-glass, polymerized
glass is a silane or siloxane compound thats been dissolved in
an alcohol- based solvent like ethanol, says Jacobsen. It leaves a
thin glassy coating that helps bind the silica to particle surfaces.

Illustration by C. Cain, adapted from S. Jacobsen

Silica has been a staple in professionally engineered germ warfare powders
for decades. (The Soviet Union added to its powders resin and a silica dust
called Aerosil a formulation requiring high heat to create nanoparticles,
says Alibek. U.S. labs have tested an Aerosil variant called Cab-O-Sil, and
declassified U.S. intelligence reports state that Iraqs chemical and
biological warfare labs imported tons of both Cab-O-Sil and Aerosil, also
known as solid smoke, in the 1980s). If theres
polymerized glass [in the Senate samples], it really narrows the field [of
possible suspects], says Jacobsen, who has been following the anthrax
investigations keenly. Polymerized glasses are exotic materials, and
nanotechnology is something you just dont do in your basement.

By March 2002, federal investigators had lab results indicating that the
Senate anthrax spores were treated with polymerized glass, and stories began
to appear in the media. CNN reported an unusual coating on the
spores, and Newsweek referred to a chemical compound that was
unknown to experts who have worked in the field for years. When
Science asked the FBI about the presence of polymerized glass in the
Senate powder, an FBI spokesperson said the bureau could not comment
on an ongoing investigation.

About-face

By the fall of 2002, the awe-inspiring anthrax of the previous spring had
morphed into something decidedly less fearsome. According to sources on Capitol
Hill, FBI scientists now reported that there was no additive
in the Senate anthrax at all. Alibek said he examined electron micrographs
of the anthrax spores sent to Senator Daschle and saw no silica. But
I couldnt be absolutely sure, Alibek says, because I only
saw three to five of these electron micrographs. Even the astonishingly
uniform particle size of 1.5 to 3 micrometers, mentioned in 2001 by Senator
Bill Frist (RTN), now included whopping 100-micrometer agglomerates,
according to the new FBI description recounted by Capitol Hill aides. The
reversal was so extreme that the former chief biological weapons inspector
for the United Nations Special Commission, Richard Spertzel, found it hard
to accept. No silica, big particles, manual milling, he says:
Thats what theyre saying now, and that radically contradicts
everything we were told during the first year of this investigation.

Military scientists did not back off their findings. The August/October 2002
newsletter from the Armed Forces Institute of Pathology (AFIP) reported that
a mass spectrometry analysis found silica in the powder sent to Senator Daschle
(The AFIP Letter, August/October 2002, p. 6). This was a key
component, said the institutes deputy director, Florabel Mullick,
in the AFIP newsletter. Silica prevents the anthrax from aggregating,
making it easier to aerosolize, she added. Frank Johnson, chief of
AFIPs Chemical Pathology Division, corroborated this in an interview.
There was silica there, said Johnson, there was no mistaking
it. Maj. Gen. John S. Parker, commander of the U.S. Army Medical Research
and Materiel Command at the time of the attacks, says he saw AFIPs
lab reports. There was a huge silicon spike consistent with the
presence of silica, he says. It peaked near the top of the screen.

Other agencies support this view today. For example, John Cicmanec, a scientist
with the U.S. Environmental Protection Agency, says the Department of Homeland
Security confirmed to EPA that the perpetrators did, in fact, use silica
to weaponize the Senate anthrax spores. According to an abstract that Cicmanec
will present at the annual meeting of the Society for Risk Analysis next
month, this weaponized form of anthrax is more than 500 times more lethal
than untreated spores.

The contradictory military data compelled the FBI to do some explaining.
Sources on Capitol Hill say that in an FBI background briefing given in late
2002, Dwight Adams, one of the FBIs topranking scientists, suggested
that the silica discovered in the Senate anthrax was, in fact, silicon that
occurred naturally in the organisms subsurface spore coats. To support
his thesis, Adams cited a 1980 paper published by the Journal of
Bacteriologya paper that Matthew Meselson, a molecular biologist
at Harvard University, says he sent to the FBI. The authors reported that
they found silicon, the element, in the spore coats of a bacterium called
B. cereus, a close cousin of anthrax.

In the 23 years since the Journal of Bacteriology published these
data, however, no other laboratory has published a report on significant
amounts of silicon in the B. cereus spore coat, and many bacteriologists
familiar with these data consider them an anomaly. Even the authors suggested
the finding might have been due to contamination.

In December 2002, the FBI decided to test whether a high-grade anthrax powder
resembling the one mailed to the Senate could be made on a small budget,
and without silica. To do this job, the bureau called upon Army scientists
at Dugway Proving Ground, a desolate Army test range in southwestern Utah.
By February 2003, the scientists at Dugway had finished their work. According
to military sources with firsthand knowledge of this effort, the resulting
powder flew like penguins. The experiment had failed. (Penguins
cant fly.)

Military sources say that Dugway washed and centrifuged the material four
times to create a pure spore preparation, then dried it by solvent extraction
and azeotropic distillation a process developed by the U.S. Chemical
Corps at Fort Detrick in the late 1950s. It is not a simple method, but someone
familiar with it might be able to jury-rig a lab to get the job done. As
recently as 1996, Bill Patrick says he taught scientists at Dugway how to
do this.

The FBI-Dugway effort produced a coarse powder. The sporessome dried
under an infrared lamp and the others airdried stuck together in little
cakes, according to military sources, and then were sieved through a
fine steel mesh. The resulting powder was placed into test tubes. When
FBI officials arrived at Dugway to examine the results, a Dugway scientist
shook one of the tubes. Unlike the electrostatically charged Senate anthrax
spores that floated freely, the Dugway spores fell to the bottom of the test
tube and stayed there. That tells you the particles were too big,
says Spertzel. It confirms what Ive been saying all along: To
make a good powder, you need an additive.

Close to home

One doesnt have to look very far to find a powder that more closely
resembles the Senate anthrax. The U.S. Armys newest batch of anthrax
simulant is a closer match, made with B. globigii (BG) spores, which
are similar to anthrax but nonlethal. According to military sources, the
Danish company Chris-Hansen spray-dried the spores (along with an unidentified
additive) in Valby, a suburb of Copenhagen. Although the spore
count varied somewhat from batch to batch, Chris-Hansen says that the average
concentration was 500 billion spores per gram, about 100 times more concentrated
than the Armys old BG powder. Chris-Hansen shipped the bulk material
from Denmark to its New Berlin, Wisconsin, facility in 1996, where, according
to Army instructions, it mixed silica into the powdera product sold
commercially under the name Sipernat D 13. Sipernat D 13 is made by
Germanys Degussa AG, the same company that makes Aerosil.

The initial Chris-Hansen production run wasnt exactly what the Army
wanted, military sources say, so this batch of anthrax simulant was further
enhanced at Dugway Proving Ground. An official at Chris-Hansen, speaking
on condition of anonymity, says he doesnt know if the Army added an
electrostatic charge or a coupling agent to the powder, and the Army wont
discuss it. But unlike the powder that Dugway reverse engineered earlier
this year, the most recent batch of simulantaccording to military
sourceshas great hang time.

A government scientist who had a sample of the Armys anthrax simulant
described it for Science: When he shook a test tube filled with it, a dense
fog of particles swirled to the top in roiling eddies. After 10 minutes,
the powder still hadnt settled. This scientist observed two other marked
similarities with the Senate material: There appears to be a lot of
static charge, he said. When he suspended the preparation in water,
he saw mostly single spores. When Canadian military scientists
used this silicalaced simulant in 2001 to assess the risk from anthrax spores
delivered by letter, the aerosol behaved like the one that would later
contaminate Senator Daschles office with real anthrax spores; the
weaponized BG particles spread across a 50-cubic-meter room in less than
2 minutes.

This new batch of energetic simulant was light-years beyond the
old U.S. weapon in its refinement, experts say. Divulging the specifications
of the weapon, the last foreman in charge of drying and milling anthrax spores
at Fort Detrick, Donald Schattenberg, told Science that the old U.S. anthrax
powder contained no additives. We didnt use silica or
bentonite (a clay that contains a high percentage of fine-particulate
silica), says Schattenberg. We made little freeze-dried pellets of
anthrax, he says, then we ground them down with a high-speed
colloid mill. The resulting powder contained growth media residue (called
menstruum) and vegetative cells, making it less concentrated,
according to William P. Walter, who says he worked on every batch of anthrax
spores ever produced at Fort Detrick. This extraneous material accounted
for a significant amount of the powders volume and mass.

Orley Bourland, who once managed the entire operation, says the old weapon
had no electrostatic charge and contained only 20 billion to 30 billion spores
per gram. These facts were corroborated by more than half a dozen veterans
of the former U.S. weapons program, including Edgar Bud Larson,
who scoffs at the suggestion that the Senate powder was the product of a
secret one-man operation. I think thats very unlikely,
Larson said. I dont think anyone could make this product
covertly.

So far, only Dugway Proving Ground has acknowledged making aerosols with
Ames strain spores. According to a memorandum from U.S. Army Test and Evaluation
Command dated 19 July 1995, Dugway began experiments with a liquid preparation
of the Ames strain starting in February 1994. This was part of what the Army
called bioprofiling: an effort to establish a
library of information, said the memo, to help defend against
biological attack. In December 2001, The Baltimore Sun broke the story that
Dugway had been making dried anthrax using live spores, and The Washington
Post reported that Dugway used the Ames strain in its anthrax powders. Dugway
released a statement acknowledging that its scientists have been doing this
work to develop an effective bioaerosol collection but insisted
that All anthrax used at Dugway has been accounted for.

The Battelle Memorial Institute, a nonprofit organization based in Columbus,
Ohio, is possibly the only corporation in the world known to possess both
the Ames strain as well as a national security division offering
the services of a team of engineers, chemists, microbiologists, and
aerosol scientists supported by state-of-theart laboratories to conduct research
in the fields of bioaerosol science and technology. On its Web site,
Battelle calls this research group one-of-a-kind.

As subcontractors, Battelle scientists have made anthrax powders for use
by the Army and U.S. intelligence agencies, but rarely by Fort Detrick, which
specializes in vaccine development. Charles Dasey, spokesperson for the parent
agency, the U.S. Army Medical Research and Materiel Command, says that as
far as he is aware, the only dried anthrax spores made at Fort Detrick since
it stopped making weapons were made by Battelle scientists working there
for DARPA. This material, made in a biosafety level 3 suite in the Diagnostic
Systems Division, contained killed Ames strain at a concentration of 326
million spores per gramseveral orders of magnitude less concentrated
than the Senate powder and crude by current standards.

Battelle is capable of more sophisticated work, as it also makes one of the
worlds most advanced medicinal powders. Battelles pharmaceutical
division, BattellePharma, also in Columbus, is one of the few companies anywhere
developing electrostatically charged aerosols for inhalation.
BattellePharmas Web site boasts that the companys new
electrohydrodynamic aerosol reliably delivers more than
80% of the drug to the lungs in a soft (isokinetic) cloud of uniformly sized
particles. Other powders, boasts the Web site, only achieve 20% or
less.

None of this argues that Battelle or any of its employees made the Senate
anthrax powder. But it is evidence that Battelle was a logical place to start
looking for clues. Officials from Battelle and the Army declined to comment
on any aspect of anthrax powder manufacture.

The FBI says it has interviewed and polygraphed scientists working at both
Dugway and Battelle. No person of interest at either facility
has been named, and no evidence has been made public indicating either as
a point of origin.

A dose of reality

Today, there is no firm evidence to link Iraqor any other
governmentto the anthrax attacks. But some weapons experts such as
Spertzel are still inclined to look for a sponsor with deep pockets, and
they say Husseins regime cannot be ruled out. Spertzels main
point, however, is that only a state-run facility or a corporation has the
resources to make an anthrax powder as good as the one mailed to the Senate.

The amateur anthrax scenario appears to have lost some credibility with the
failure of the FBIs attempt to reverse engineer a highquality powder
using basic equipment. If the Army couldnt do it in a top-notch laboratory
staffed by scientists trained to make anthrax powders, skeptics ask, who
could do it in a garage or basement?

The silica dust might still provide a trail to the killers, say chemists
who specialize in silica. According to military sources, since the abandonment
of the offensive biological warfare program, the U.S. Army has continued
to experiment with various brands of silica nanoparticles added to germ-warfare
powders produced in small quantities. These include WR-50 and WR-51 (manufactured
by Philadelphia Quartz Co.), Cab-O-Sil (Cabot Corp.), and Sipernat D 13 (Degussa
AG). Each brand is made differently, so each has a unique chemical signature,
says Jonathan L. Bass, a Pennsylvania-based analytical chemist who used to
do research with silica at PQ Corp. (formerly Philadelphia Quartz).
Itd be a laborious process, and some of the differences would
be hard to detect, says Bass, but if a known brand of silica
was used by the killers, I think I could trace it back to a specific
company. A coupling agent should also provide a unique chemical signature
that could narrow the field.

Two years on from the attacks, public discussion of the silica additive has
all but ceased; the discussion about polymerized glass has yet to occur.
Instead, the FBI has devoted much of its effort to the idea that a low-budget
amateur operation could have produced a weaponized form of anthrax
powder without a sophisticated additive.

ARE YOU AFRAID? asked our unknown assailants 2 years ago.
Yes, is still the answer, but of whom?