Abstract

The rice cultivar ‘Chumroo’ is commonly cultivated in the mid- and high-altitude areas of Bhutan. This cultivar has shown durable blast resistance in that area, without evidence of breakdown, for over 20 years. Chumroo was inoculated with 22 blast isolates selected from the race differential standard set of Japan. The cultivar showed resistance to all the isolates. To identify the resistance gene(s), Chumroo was crossed with a susceptible rice cultivar, Koshihikari. The F1 plants of the cross showed resistance. Segregation analyses of 300 F3 family lines fitted the segregation ratio of 1:2:1 and indicated that a single dominant gene controls the resistance to a blast isolate Ao 92-06-2 (race 337.1). The Chumroo resistance locus (termed Pi46(t)) was mapped between two SSR markers, RM6748 and RM5473, on the terminal region of the long arm of chromosome 4, using linkage analysis with SSR markers. The nearest marker, RM5473, was linked to the putative resistance locus at a map distance of 3.2 cM. At the chromosomal region, no true resistance genes were identified, whereas two field resistance genes were present. Therefore, we designated Pi46(t) as a novel blast resistance locus.

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Acknowledgments

We thank Dr. T. Nagamine and Dr. T. Ishii, National Agricultural Research Center for Western Region (WeNARC), Dr. M. Kawase, National Institute of Agrobiological Sciences, and Dr. K. Matsuya, Japan International Cooperation Agency for their technical advice. We also thank Dr. E. Araki, WeNARC, for providing the SSR markers. We also thank the field technicians of the Technical Support Section, WeNARC, who provided the plant materials. We also thank Ms. N. Kobayashi, Ms. I. Ikeda, Ms. K. Hanada, Ms. Y. Tashima, and Ms. M. Oda for their technical assistance.

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