The UNE-EN 14675 test uses bovine Enterovirus type 1 as an obligate organism. Additional optional viruses proposed by the client may be used, as in this case, virucidal assay with the Infectious Salmon Anemia Virus (ISA), a disease caused by a virus of the family Orthomyxoviridae, genus Isavirus. The clinical disease affects fish grown in sea water, Salmo salar species (Atlantic Salmon).

The test must be carried out at a mandatory temperature of 10ºC and for a mandatory time of 30 minutes. Optional additional temperatures may be chosen as 4°C, 20°C or 40°C, and as optional additional times 1, 5 or 60 minutes may be used. The test can be performed under simulated conditions of low dirtiness or in simulated conditions of high dirtiness. The product should be tested at least three concentrations including one in the active range and one in the non-active range (these last two concentrations are chosen by the laboratory that performs the test since they correspond to the positive and negative controls, respectively). To consider the virucidal product a logarithmic reduction of 4 in infectivity must be demonstrated.

Note 1: For those optional virus not mandatory by the standard guideline, to be under accreditation, it is required that the test be performed at the same time with any of the mandatory viruses.