Custom antibody development

We offer world-leading antibody development platforms based on a proprietary RabMAb® rabbit monoclonal platform, a phase display platform (AxioMx), and a next-generation sequencing platform (NGS-RabMAb®)

Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

ICC/IF

1/250.

ICC

1/1000.

WB

1/500. Predicted molecular weight: 46.5 kDa.

Target

Function

Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.

Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.Ubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation.

Thank you for your reply. A549 cells were recommended because they showed weak to no staining using a similar antibody. Plus these are the results found in the Human Protein Atlas: http://www.proteinatlas.org/ENSG00000169252/cell/A-549 I'm not sure if...

Thank you for your reply and for providing the requested information.
After seeing the data and your protocol, I would like to make some suggestions that may improve your results. One of my colleagues who has worked with chicken antibodies in the p...