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Notes: pCAT promoter constructs were used to evaluate the effects of IL-10 and IGF-I on MMP-2 expression.The CAT assays were performed using the CAT Enzyme Assay System with Reporter Lysis Buffer. (2637)

Notes: The authors used the pCAT® Basic and pGL3 Basic Vectors to assay for various slow and fast twitch type troponin I transcriptional regulatory elements. They co-transfected pGL3 Basic-derived Vectors with pRL-SV40 Vector at a ratio of 25:1 using calcium phosphate method. Assayed luciferase activities using Dual-Luciferase® Reporter Assay System in a Berthold luminometer. (1383)

Notes: Reporter studies were performed in rat vascular smooth muscle cells. Experimental constructs were prepare in the pCAT®-3 Basic Vector. Controls included co-transfection with a beta-galactosidase expression vector. Results are reported in relation to the activity shown by the pCAT®-3 Control Vector. (0404)

Notes: Reporter studies were performed in rat hepatoma cell line, MH1C1. Experimental constructs were prepared in the pCAT®-3 Basic Vector. Transfections were control by a cotransfection of the pSV-β-Galactosidase Control Vector. (1218)

Notes: The authors used the pCAT® Basic Vector in their promoter studies and RQ1 RNase-free DNase and rhSP1 in their footprinting studies with Drosophila SL2 cells. Promega's pCAT Vectors have now been replaced by the next generation pCAT® 3 Reporter Vectors. (0407)

Notes: The authors used the Primer Extension System to map the transcriptional start site of human CCR5 gene. They cloned the putative CCR5 promoter, and regions thereof were cloned into the CAT in pCAT®-Basic Vector. Transient transfections were controlled for using a β-galactosidase expression plasmid, and β-galactosidase levels assayed using the β-Galactosidase Assay System. The pCAT® Vectors have been replaced by the next generation pCAT®3 Vectors. (1085)

Notes: CAT activity was detected in the MPT mouse kidney cell line and constructs were prepared in the pCAT® Promoter Vector. To demonstrate that the promoter of interest only functioned in kidney cells, a transgenic line was established with beta-galactosidase under the control of the promoter. RT-PCR could only detect expression in the kidney of beta-galactosidase but detected G3PDH in all tissues examined. The promoter of interest could gel shift with rhAP-2, and rhAP-2 could protect a 45bp region of the promoter in a footprinting experiment. (0423)

Notes: Promoter studies were performed in cultured rat aortic smooth muscle cells, and constructs were made in the pCAT®-Basic Vector. The Altered Sites® II in vitro Mutagenesis System was used to mutagenize the TGFβ control element. The recombinant human SP1 was used for footprinting. The pCAT® Vectors have been replaced by the next-generation pCAT®3 Vectors. (1069)

J. Biol. Chem.272, 21045-21051.
A type I interferon signaling factor, ISF21, encoded on chromosome 21 is distinct from receptor components and their down-regulation and is necessary for transcriptional activation of interferon-regulated genes.1997

Notes: Luciferase and CAT assays were conducted in CHO-K1 cells using constructs prepared in the pGL3-Basic and pCAT®-Basic Vectors, and the activity was normalized to β-galactosidase activity supplied by the pSV-β-Galactosidase Control Vector. The pCAT® Vectors have been replaced by the next-generation pCAT®3 Vectors. (1055)

J. Biol. Chem.272, 3674-3682..
Expression of the 90K immunostimulator gene is controlled by a promoter with unique features.1997

Brakebusch, C., Jallal, B., Fusco, O., Iacobelli, S., Ullrich, A.

Notes: CAT reporter studies were performed in NIH3T3 cells using the pCAT® Basic Vector, pCAT® Promoter Vector and the CAT Enzyme Assay System. (Promega's pCAT Vectors have now been replaced with the improved pCAT®-3 Vector series). (1399)

Notes: Reporter studies were performed in rat aortic smooth muscle cells and rat L6 skeletal myoblasts and myotubes. Reporter constructs were prepared in the pCAT®-Basic Vector and the pCAT®-Control Vector was employed as well. (Please note: the pCAT® Vectors have been discontinued and replaced with the next generation CAT vectors, the pCAT®3 Vectors.) (0722)

Notes: Reporter studies were performed in the neural cell lines, CG-4 and PC-12, and the non-neural cell lines, HeLa and NIH3T3, using constructs prepared in the pCAT®-Basic Vector. The pCAT® Vectors have been replaced with the next generation pCAT®3 Vectors. (1027)

J. Biol. Chem.272, 3109-3116.
Induction of the intronic enhancer of the human ciliary neurotrophic factor receptor (CNTFRalpha) gene by the TR4 orphan receptor: A member of steroid receptor superfamily.1997

Young, W.J., Smith, S.M. and Chang, C.

Notes: The TNT® Coupled Rabbit Reticulocyte Lysate System was used for invitro translation of protein from a cDNA clone. This in vitro translated protein was used for gel shift assays and Scatchard analysis of the DNA-TR4 protein interaction. The pCAT® Promoter Vector was used to assay the enhancer activities of the intronic region. (1537)

Notes: Studies were performed in a Burkitt lymphoma cell line designated Daudi using the pCAT®-Basic and -Control Vectors. pCAT® Vectors have been replaced by the next generation pCAT®3 Vectors. (0963)

J. Clin. Invest.99, 1361-1366.
Plasma lipoprotein(a) levels and expression of the apolipoprotein(a) gene are dependent on the nucleotide polymorphisms in its 5'-flanking region.1997

Suzuki, K. , Kuriyama, M. , Saito, T. , Ichinose, A.

Notes: The authors used the pCAT® Basic and Enhancer Vectors as well as the pSV β-Galactosidase Control Vector in the study. Promega's pCAT® Vectors have now been replaced by the improved pCAT® -3 Vector series. (0281)

Notes: CAT reporter studies were performed in FRTL-5 rat thyroid cells and the constructs were prepared in the pCAT® Basic and pCAT® Promoter Vectors. Promega's pCAT Vectors have now been replaced by the next generation pCAT® 3 Reporter Vectors. (0471)

J. Biol. Chem.272, 5899-5908.
Regulation of murine cytochrome oxidase Vb gene expression in different tissues and during myogenesis. Role of a YY-1 factor-binding negative enhancer.1997

Basu, A., Lenka, N., Mullick, J. and Avadhani, N.G.

Notes: Reporter studies were performed in 3T3, COS, Hep3B and C2C12 cells using the pCAT® Basic Vector. The Erase-A-Base® System was used to make deletion mutants of the promoter being studied. (An improved version of the pCAT® Basic Vector, the pCAT® 3 Basic Vector is now available) (1466)

Notes: Promega's pCAT® Basic and pSV Beta-Galactosidase Vectors were used in this study. Beta-galactosidase activity was assayed with the Beta-Galactosidase Enzyme Assay System with Reporter Lysis Buffer. Promega's pCAT Vectors have now been replaced by the next generation pCAT® 3 Reporter Vectors. (0434)

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