The Fish DNA Barcoding Kit is an advanced PCR kit that allows students to determine the species of a fish sample based on its DNA sequence of the cytochrome c oxidase I gene.

Key Features and Benefits

With this kit, students are able to:

Perform DNA extraction, PCR amplification, and gel electrophoresis

Use sequencing and bioinformatics to determine fish species

Contribute to the global initiatives to barcode life

Background Information

Species are traditionally identified by taxonomists who need to have in-depth knowledge of certain species characteristics and environments in order to categorize them. The power of DNA research now allows us to add a level of identification based on the sequencing of a conserved gene. The subtle changes in sequences result in species-specific identification or genetic barcodes. This information is critical in helping to assess biodiversity and conservation efforts, as well as commercially, where food marketplaces can be regulated.

More Information

This kit provides sufficient materials for processing up to 16 fish and control samples (8 student work stations, 2 samples per workstation, 2–4 students per workstation).

The DNA Barcoding Sequencing Module allows you to order sequencing services now and use them when you’re ready. Sample shipping to a validated sequencing facility is included with this module! PCR cleanup and sequencing are included for up to nine samples per sequencing module.

This kit provides sufficient materials for processing up to 16 fish samples (8 student work stations, 2 samples per workstation, 2–4 students per workstation).

Cell resuspension solution, 5 ml

1

Cell lysis solution, 5 ml

1

Neutralization solution, 5 ml

1

Wash buffer, 10 ml

1

Matrix, 5 ml

1

Spin filters

20

PCR tubes, 0.2 ml

50

Microcentrifuge tubes, 2 ml

250

PCR master mix (Taq DNA polymerase, dNTPs, buffer), 1.2 ml

1

Fish primer mix (40x), 50 µl

1

pCOI control, 25 µl

1

UView™ 6x loading dye, 200 µl

1

PCR molecular weight ruler, 200 µl

1

Sterile water, 2.5 ml

1

Curriculum

1 (includes teacher guide, student manual, and graphic quick guide)

DNA Barcoding Sequencing Module

PCR cleanup and sequencing for up to 9 samples

1 set for up to 9 samples

What is the education discount policy?

For more than 15 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows high school and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices.

How do I apply for the education discount?

If you are an educator at the high school or college level, visit our Education Discount Policy page to establish an education account number.

166-5100EDU
Advanced PCR and bioinformatics kit, includes reagents for DNA extraction, PCR amplification, plastics, curriculum, for processing up to 16 fish and control samples (8 student work stations, 2 samples per workstation, 2–4 students per workstation); education use only; sequencing module sold separately

166-5115EDU
Sequencing module, PCR cleanup and DNA sequencing for up to 9 fish samples; prepaid service that can be claimed with an authorized sequencing vendor, sample shipping included; 2 modules required to serve 1 full Fish DNA Barcoding Kit (#166-5100EDU); education use only