Principle of the measurement

Cell permeable HYDROP™ hydrolyzed by intracellular esterases to yield a material that is impermeable to the cell membrane and thus stays within the cell. The hydrolysis also increases the reactivity with hydrogen peroxide. HYDROP and its hydrolyzed product are almost non-fluorescent in neutral solutions. After the reaction with hydrogen peroxide, it generates a highly fluorescent product (excitation max: 492 nm, emission max: 518 nm).

ROS Selectivity

Figure 1. Reaction of HYDROP™ to various ROS

Fluorescence increase of hydrolyzed HYDROP™ is observed by the addition of hydrogen peroxide at physiological pH of around 7.4 or higher.