To ensure highest sensitivity, artus Parvo B19 PCR Kits have been optimized to detect low numbers of parvovirus B19 DNA. The analytical sensitivity of the artus Parvo B19 RG PCR Kit is 0.2 IU/µl in the PCR on the Rotor-Gene 3000.

Performance characteristics of artus Parvo B19 RG PCR Kit

Kit

artus Parvo B19 RG PCR Kit

Validated sample types

Serum or plasma

Analytical sensitivity

0.2 IU/µl in the PCR

Principle

artus Parvo B19 PCR Kits are based on the amplification and simultaneous detection of a specific region of the parvovirus B19 genome using real-time PCR. The kits provide high levels of specificity, sensitivity, and reproducibility.

Each artus Parvo B19 PCR Kit provides 5 quantitation standards (see figure "Reliable quantitation of parvovirus B19 load"). Use of the standards enables accurate quantitation of viral load. In addition, the kits contain a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical parvovirus B19 PCR is not reduced.

For DNA purification, the QIAamp DNA Mini Kit or QIAamp UltraSens Virus Kit is to be used.

Applications

artus Parvo B19 PCR Kits enable the rapid and sensitive detection of parvovirus B19 DNA from serum and plasma with accurate quantification. The kits are available for use on the Rotor-Gene 3000, the LightCycler 1.1/1.2/1.5 Instruments, or the ABI PRISM 7000, 7700, 7900HT SDS.