<Dear colleagues,
we use DMSO (10% final conc) as cryoprotector to keep E. coli
cultures ( our gene library) at -80 degree Celcius.
Please, tell me if you had that experience maintaining the
libraries- E. coli cultures - how many times I can freeze and defrost
the cultures and they to be still viable?
Maybe the freezing-defrosting procedure is also of importance -
slow or fast ?
Thank you,
Ani