Bottom Line:
It exhibited increase in maximum root length without any significant changes in its root weight, root volume and total root number on crown when compared with the WT under stress in PVC tube experiment.Root anatomy and stomatal microscopic studies revealed changes in the number of xylem and phloem cells, size of central meta-xylem and number of closed stomata in ewst1.The possible involvement of a candidate gene with respect to the observed morpho-physiological and transcriptional changes and its role in stress tolerance are discussed.

Mentions:
To identify mutant and stress-specific DEGs from the entire gene expression profile generated, a six-way Venn diagram was prepared (Fig. 6A). Out of 57 381 array probes, 16 939 probes (29.5 %) were significantly hybridized and 7534 probes were differentially expressed at ≥2-fold change (P < 0.05) in any one of the six possible combinations (MS vs MC, MC vs NC, NS vs MC, MS vs NC, MS vs NS and NS vs NC). The numbers of up- and down-regulated DEGs are presented in Fig. 6B and the detailed gene list is given in Supporting Information —Table S2. The Venn analysis identified a total of 873 genes forming 12 clusters of similar expression pattern (up- and down-regulated DEGs in six groups). The number of up- and down-regulated DEGs (Fig. 6C) and the detailed gene list have been given in the Supporting Information—Table S3. We have termed these genes as URDEGs. The URDEGs were again subcategorized into up-regulated and down-regulated classes based on their expression in the mutant under control or stress conditions when compared with the WT. The URDEGs, which were up-regulated in the WT were considered as repressed genes in the mutant, while the down-regulated URDEGs in WT were considered as activated genes in the mutant. The heatmaps of URDEGs of these clusters represented the same expression pattern as analysed by our method (Fig. 7). A total of 348 genes showed differential expression specifically under control conditions, while 443 genes did so specifically under stress. However, only 85 genes were found to show differential expression in the mutant under both stress and control conditions when compared with the WT.Figure 6.

Mentions:
To identify mutant and stress-specific DEGs from the entire gene expression profile generated, a six-way Venn diagram was prepared (Fig. 6A). Out of 57 381 array probes, 16 939 probes (29.5 %) were significantly hybridized and 7534 probes were differentially expressed at ≥2-fold change (P < 0.05) in any one of the six possible combinations (MS vs MC, MC vs NC, NS vs MC, MS vs NC, MS vs NS and NS vs NC). The numbers of up- and down-regulated DEGs are presented in Fig. 6B and the detailed gene list is given in Supporting Information —Table S2. The Venn analysis identified a total of 873 genes forming 12 clusters of similar expression pattern (up- and down-regulated DEGs in six groups). The number of up- and down-regulated DEGs (Fig. 6C) and the detailed gene list have been given in the Supporting Information—Table S3. We have termed these genes as URDEGs. The URDEGs were again subcategorized into up-regulated and down-regulated classes based on their expression in the mutant under control or stress conditions when compared with the WT. The URDEGs, which were up-regulated in the WT were considered as repressed genes in the mutant, while the down-regulated URDEGs in WT were considered as activated genes in the mutant. The heatmaps of URDEGs of these clusters represented the same expression pattern as analysed by our method (Fig. 7). A total of 348 genes showed differential expression specifically under control conditions, while 443 genes did so specifically under stress. However, only 85 genes were found to show differential expression in the mutant under both stress and control conditions when compared with the WT.Figure 6.

Bottom Line:
It exhibited increase in maximum root length without any significant changes in its root weight, root volume and total root number on crown when compared with the WT under stress in PVC tube experiment.Root anatomy and stomatal microscopic studies revealed changes in the number of xylem and phloem cells, size of central meta-xylem and number of closed stomata in ewst1.The possible involvement of a candidate gene with respect to the observed morpho-physiological and transcriptional changes and its role in stress tolerance are discussed.