Monoclonal antibodies were produced from a
hybridoma resulting from the fusion of a mouse myeloma with B cells
obtained from a mouse immunized with purified, E. coli-derived,
recombinant human beta-NGF (human beta-Nerve Growth Factor).

Purification:

This IgG2b/Kappa antibody was purified by Protein
G affinity chromatography.

The lyophilized antibody is stable at room temperature for at least
one month and for greater than a year when
kept at -800C.

Reconstitution:

When reconstituted in sterile water to a concentration of
1 mg/ml the antibody is stable for at least six weeks
at 2-40C.

Applications

ELISA:

In a sandwich ELISA (assuming 100μl/well),
a concentration of 2 μg/ml of this antibody will detect at least
0.1 ng/well of recombinant human beta-NGF when used with conjunction
with PeproTechs biotinylated antigen-affinity purified anti-human
ß-NGF (500-P85Bt) as the detection antibody at a concentration
of at least 0.5 µg/ml.