Porcine Health Management

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DEEP-SEQUENCING CHARACTERIZATION OF TWO ROTAVIRUS A OUTBREAKS IN SUCKLING PIGLETS IN CATALONIA REVEALS A HIGH FREQUENCY OF RECOMBINATION EVENTS

Group A rotaviruses are one of the main causes of diarrhoea in pigs worldwide. In the present study, two outbreaks of diarrhoea in suckling pigs were investigated. Four rotavirus A isolates were deep sequenced and analysed to characterize the genetic diversity and identify recombination events.

Porcine circovirus 2 (PCV2) is a virus characterized by high evolutionary rate, which promotes the potential emergence of different genotypes and strains. Despite the likely relevance in the emergence of new PCV2 variants, the subtle evolutionary patterns of PCV2 at individual-host level or over short transmission chains are still largely unknown.

DIVERSITY OF PRRS STRAINS CIRCULATING IN CANADA

PRRS sequencing is widely used and considered essential for the control of PRRS in Canada. Through several signed agreements, a structure was put into place and has allowed achievement of near real-time transfer of all sequences to our research team from 3 different laboratories in Quebec.

PRRS VIRUS SURVEILLANCE; ROLE OF VIRUS SEQUENCING AND VIRUS DETECTION BY PCR

Porcine reproductive and respiratory syndrome (PRRS) is a highly infectious disease, endemic in pigs throughout the world. PRRS is caused by a single stranded positive-sense RNA enveloped virus with a high mutation rate leading to greater heterogeneity of the nucleotide sequence between individual strains.

THE USE OF ROUTINE SEQUENCING ACTIVITY TO MONITOR THE EPIDEMIOLOGICAL TRAJECTORIES OF PRRSV IN ITALY

Porcine reproductive and respiratory syndrome virus (PRRSV) is a major threat to European swine production and Italian pig industry. Viral evolution analysis in terms of genetic variability over time and space is one of the challenging issue in the PRRSV control mostly in swine production area characterized by high pig density, fragmentation of the production chain and lack of high biosecurity levels.

FULL GENOME CHARACTERIZATION AND PREVALENCE OF PORCINE CIRCOVIRUS TYPE 3 ISOLATES FROM GERMAN FATTENING FARMS

Recently a novel porcine circovirus called porcine circovirus type 3 (PCV3) was reported from the Americas, Asia and Europe. PCV3 was detected with high prevalence in Polish farms, but to date no genome sequences were available from European PCV3 strains. The present study was performed to estimate the PCV3 prevalence in German fattening farms and to further characterize available PCV3 isolates.

DEVELOPMENT OF A REAL-TIME RT-PCR FOR DIFFERENTIAL DETECTION OF REASSORTANT H1N2 (H1N2R) SWINE INFLUENZA VIRUS

Endemic swine influenza virus (swIAV) strains including H1avN1, H1huN2 have been circulating in Great Britain (GB) for decades. In 2009, H1N1pdm09 became the dominant strain detected followed by H1N2 and the rapid decline of H1avN1 detections. An H1N2-H1N1pdm09 virus reassortant was detected in 2010; comprising H1 haemagglutinin (HA) and N2 neuraminidase (NA) surface glycoproteins with the H1N1pdm09 internal cassette.

CHANGES OF T AND B LYMPHOCYTES IN TISSUES AND BLOOD OF PIGS EXPERIMENTALLY INFECTED WITH AN ITALIAN HIGHLY PATHOGENIC PRRSV-1.1 ISOLATE

Highly pathogenic (HP) PRRSV recently emerged in Europe. The disease is characterized by high fever and respiratory distress in young pigs and high mortality rates and reproductive failure in sows. HP-PRRSV isolates can alter the pathological outcome differently from “conventional” PRRSV, in terms of more severe clinical signs and more intense dysregulation of cellular immunity.

PCV2 can be associated with reproductive failure and cause infertility and increased rates of mummified, macerated, stillborn and weak-born piglets. The aim of this study was to determine the impact of PCV2 sow vaccination on several reproductive parameters in a Spanish sow herd.

PCV2 can be associated with reproductive failure and cause infertility and increased rates of mummified, macerated, stillborn and weak-born piglets. The aim of this study was to determine the impact of PCV2 sow vaccination on several reproductive parameters in a Spanish sow herd.

IMPROVEMENT OF REPRODUCTIVE AND PRODUCTIVE PERFORMANCE AFTER PRRS CONTROL PROGRAM IMPLEMENTATION IN SPAIN

Controlling this disease in large production systems is challenging. This is a summary of an 18 months field trial designed to evaluate the impact of the 5 step process approach using Reprocyc PRRS EU® and PRRSFlex EU® (Boehringer Ingelheim Vetmedica GmbH), a modified-live type I PRRS virus vaccine, on control of heterologous PRRSV in a commercial herd, assessed by live animal performance.

Porcine reproductive and respiratory syndrome (PRRSV) is a viral disease with negative impact on sow reproduction. An alternative to control this virus is to select animals more resilient to the infection. A key issue to deal with disease resistance is to set up a cost-efficient phenotyping strategy. The aim was to develop a phenotyping criterion to discriminate susceptible from resilient sows in endemic infected farms.

Since the identification of porcine circovirus 2, the Circovirus genus has become of major relevance, especially for its impact in the swine industry. Recently, a new species (Porcine circovirus 3, PCV-3) has been detected in healthy and diseased pigs. Consequently, there is an urgent need for reliable and widely accessible diagnostic tools for both routine diagnosis and research purposes.

THE HIGH WITHIN-EUROPE PORCINE CIRCOVIRUS 3 GENETIC HETEROGENEITY: FULL GENOME SEQUENCING OF FIELDS STRAINS FROM DENMARK, ITALY AND SPAIN

Porcine circovirus 3 (PCV3) is a new species of the Circovirus genus, which has recently been detected in healthy and diseased pigs. Its presence has been reported in different countries of North and South America, Asia and recently also Europe (Poland and Italy). However, little is known about PCV3 molecular epidemiology and transmission patterns in Europe.

ResPig®program includes biannual serological and saliva-PCR PRRS tests of all production groups. PRRSv is further characterized upon request. PRRSv prevalence and dynamics is summarized in the database.

European Association of Porcine Health Management

The European Association for Porcine Health Management (EAPHM) is a community that actively exchanges experiences and ideas around Porcine Health Management. Our members are pig veterinarians in Europe. We provide European practitioners with a platform for sharing information and developing and improving their skills and competences.