Enz-Flow: Continuous Bioprocessing for Greener API Synthesis

The union of two enabling and increasingly environmentally-friendly technologies, continuous processing and continuous bioprocessing, is here applied (“Enz-Flow”) towards novel abbreviated and more sustainable syntheses of three active pharmaceutical ingredients (APIs). Chirally-pure formulations of all three of these APIs have been demonstrated to possess higher activity than the racemic mixtures of these compounds. Continuous processing facilitates abbreviated and telescoped syntheses of these molecules, and continuous bioprocessing affords highly selective stereochemical control. The work presented here will thus demonstrate the utility of applying continuous processing and continuous bioprocessing strategies for shortening and improving sustainability of API synthesis.