Mashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-387420220170201AdipoRon may be benefit for atherosclerosis prevention107109ENMaryamEsfahaniDepartment of Biochemistry and Nutrition, Hamadan University of Medical Sciences, Hamadān, Iranesfahanimr21@gmail.comNooshinShababResearch Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadān, Irannnshabab2@gmail.comMassoudSaidijamDepartment of Molecular Medicine and Genetics, Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadān, Iransjam110@yahoo.com10.22038/ijbms.2017.8228Atherosclerosis has serious role in coronary arteries disease,so it is important to establish effective strategies for prevention or even treatment of atherosclerosis. Adiponectin, as one of the most abundant adipokines, has insulin sensitivity, anti-inflammatory and anti-atherogenic properties. Disturbed adiponectin actions through its receptor, (AdipoR1 and AdipoR2) may be involved in atherosclerosis development. Some adiponectin effects are mediated by AMPK and PPAR-α signaling. AdipoRon is an orally active synthetic molecule which can bind to AdipoR1, Adipo R2 and activate them. AdipoRon can activate AdipoR1-AMPK- PGC-1α pathway and AdipoR2-PPAR-α pathway. Some studies indicated insulin sensitivity, anti-apoptotic and anti-oxidative effect of AdipoRon. We hypothesize that AdipoRon has anti atherosclerotic effect and may suppress atherosclerosis processes. With confirmation the benefit role of AdipoRon on atherosclerosis, it may be used in patients at risk of atherosclerotic development.Adiponectin,Adiponectin receptors,AdipoRon,Atherosclerosis,Cardiovascular Diseasehttp://ijbms.mums.ac.ir/article_8228.htmlhttp://ijbms.mums.ac.ir/article_8228_5728c7eebc0d29a0e2851bf8f3f33fa5.pdfMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-387420220170201Toxicology effects of saffron and its constituents: a review110121ENHasanBadie BostanDepartment of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iranbadieh931@mums.ac.irSoghraMehriDepartment of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran|Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran|Neurocognitive Research Center, Mashhad University of Medical Sciences, Mashhad, Iranmmehri86@yahoo.comHosseinHosseinzadehDepartment of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran|Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iranhoseinzadehh@mums.ac.ir10.22038/ijbms.2017.8230Saffron (Crocus sativus L.) has been considered as a medicinal plant since ancient times and also widely used as food additive for its color, taste and odor. The pharmacological properties of saffron and its main constituents, crocin and safranal have been evaluated using different in vivo and in vitro models. Additionally, other lines of studies have found toxicological effects of saffron. However, a comprehensive review that covers all aspects of its toxicity has not been published yet. The current study provides classified information about the toxic effects of saffron and its constituents in various exposure conditions including acute, sub-acute, sub-chronic and chronic studies. Therapeutic doses of saffron exhibits no significant toxicity in both clinical and experimental investigations.Crocetin,Crocin,Saffron,Safranal,toxicityhttp://ijbms.mums.ac.ir/article_8230.htmlhttp://ijbms.mums.ac.ir/article_8230_5bf447fc2fe20c473e8024c504bee251.pdfMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-387420220170201CFP10: mFcγ2 as a novel tuberculosis vaccine candidate increases immune response in mouse122130ENAli AsgharBaghaniAntimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iransoleimanpour.saman@yahoo.comSamanSoleimanpourAntimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iransoleimanpours@mums.ac.irHadiFarsianiAntimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iranfarsianih@mums.ac.irArmanMosavatHIV/AIDS, HTLV and Viral Hepatitis Research Center, Iranian Academic Center for Education, Culture and Research (ACECR), Mashhad, Iranmosavata881@mums.ac.irMasoudYousefiAntimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranZahraMeshkatAntimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranSeyed AbdolrahimRezaeeImmunology Research Center, Inflammation and Inflammatory Diseases Division, Medical school, Mashhad University of Medical Sciences, Mashhad, IranSaeidAmel JamehdarAntimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iranameljs@muma.ac.irMohammad RezaAkbari EydgahiBiotechnology Research Center, Semnan University of Medical Sciences, Semnan, Iranmrakbari201177@yahoo.comHamidSadeghianOrganic Chemistry, Department of Laboratory Sciences, Mashhad University of Medical Sciences, Mashhad, IranKiarashGhazviniAntimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iranghazvinik@mums.ac.ir10.22038/ijbms.2017.8231Objective(s): Despite treatment with antibiotics and vaccination with BCG, tuberculosis (TB) is still considered as one of the most important public health problems in the world. Therefore, designing and producing a more effective vaccine against TB seems urgently. In this study, immunogenicity of a fusion protein which consisting or comprising CFP-10 from Mycobacterium tuberculosis and the Fc-domain of mouse IgG2a was evaluated as a novel subunit vaccine candidate against TB. Materials and Methods: The genetic constructs were cloned in pPICZαA expression vector and recombinant vectors (pPICZαA-CFP-10: Fcγ2a and pPICZαA-CFP-10:His) were transformed into Pichia pastoris. To evaluate the expression of recombinant proteins, SDS-PAGE and immunoblotting were used. The immunogenicity of recombinant proteins, with and without BCG were assessed in BALB/c mice and specific cytokines against recombinant proteins (IFN-γ, IL-12, IL-4, IL-17 and TGF-β) were evaluated. Results: The levels of IFN-γ and IL-12 in mice that received recombinant proteins was higher than the control groups (BCG and PBS). Thus, both recombinant proteins (CFP-10:Fcγ2a and CFP-10:His) could excite good response in Th1-cells. The Fc-tagged protein had a stronger Th1 response with low levels of IL-4, as compared to CFP-10:His. However, the highest level of Th1 response was observed in groups that were vaccinated with BCG (prime) and then received recombinant protein CFP-10: Fcγ2a (booster). Conclusion: The results demonstrated that binding mice Fc-domain to CFP-10 protein can increase the immunogenicity of the subunit vaccine. Further studies, might be able to design and produce a new generation of subunit vaccines based on the Fc-fused immunogen.CFP-10,Fcγ2a,Immunogenicity,Mycobacterium tuberculosis,Subunit vaccinehttp://ijbms.mums.ac.ir/article_8231.htmlhttp://ijbms.mums.ac.ir/article_8231_564b4fba2fca6fd9f17d6ec263b8b059.pdfMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-387420220170201Protective role of licochalcone B against ethanol-induced hepatotoxicity through regulation of Erk signaling131137ENXiao-pengGaoDepartment of General Surgery, Xi’an Central Hospital, The Affiliated Xi'an Central Hospital of Xi'an Jiaotong University College of Medicine, Xi'an 710003,P.R.Chinagaoxiaopengmed@163.comDong-weiQianDepartment of Operation Room, Xi’an Central Hospital, The affiliated Xi'an central hospital of Xi'an Jiaotong university College of Medicine, Xi'an 710003,P.R.Chinaqiandongweimed@sina.comZhenXieDepartment Two of Neurology, Shaanxi Provincial People’s Hospital, Xi’an, ChinaHaoHuiDepartment Two of Neurology, Shaanxi Provincial People’s Hospital, Xi’an, China10.22038/ijbms.2017.8235Objective(s): Oxidative stress has been established as a key cause of alcohol-induced hepatotoxicity. Licochalcone B, an extract of licorice root, has shown antioxidative properties. This study was to investigate the effects and mechanisms of licochalcone B in ethanol-induced hepatic injury in an in vitro study. Materials and Methods: An in vitro model of Ethanol-induced cytotoxicity in BRL cells was used in this study. Cell injury was assessed using WST-1 assay and lactate dehydrogenase, alanine transaminase, and aspartate aminotransferase release assay. Cell apoptosis were quantified by flow cytometric analysis. The intracellular oxidative level was evaluated by reactive oxidative species, malondialdehyde and glutathione detection. Furthermore, the expression level of Erk, p-Erk, Nrf-2 were assessed using Western blot. Results: Treatment with ethanol induced marked cell injury and cell apoptosis in BRL cells. Licochalcone B significantly attenuated ethanol-induced cell injury, and inhibited cell apoptosis. Furthermore, licochalcone B significantly inhibited ethanol-induced intracellular oxidative level, upregulated the expression of p-Erk, and promoted nuclear localization of Nrf2. Additionally, this hepatoprotective role was significantly abolished by inhibition of Erk signaling. However, no apparent effects of Erk inhibition were observed on ethanol-induced hepatotoxicity. Conclusion: This study demonstrates that licochalcone B protects hepatocyte from alcohol-induced cell injury, and this hepatoprotective role might be attributable to apoptosis reduction, inhibition of oxidative stress, and upregulation of Erk–Nrf2. Therefore, licochalcone B might possess potential as a novel therapeutic drug candidate for alcohol-related liver disorders.Alcohol,BRL cells,Erk,Hepatotoxicity,Nrf,Oxidative Stresshttp://ijbms.mums.ac.ir/article_8235.htmlhttp://ijbms.mums.ac.ir/article_8235_cd02bcab4f7a091695514e03be1d07f2.pdfMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-387420220170201Exploring the effect of intravenous lipid emulsion in acute methamphetamine toxicity138144ENAmenehGhadiriDepartment of Toxicology, Faculty of Pharmacy, Islamic Azad University, Shahreza Branch, Shahreza, Iranghadiria64@gmail.comLeilaEtemadPharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iranetemadl@mums.ac.irMohammadMoshiriLegal Medicine Research Center, Legal Medicine Organization, Tehran, Iranmoshiri.mo@gmail.comSeyed AdelMoallemDepartment of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iransamoallem@yahoo.comAmir HosseinJafarianDepartment of Pathology, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iranjafarian.a@chmail.irFarzinHadizadehBiotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iranfhadizadeh@yahoo.comMahmoudSeifiBiotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iranseifim@mums.ac.ir10.22038/ijbms.2017.8236Objective(s): The increasing use of methamphetamine (METH) in the last decades has made it the second most abused drug. Advancs in the area of intravenous lipid emulsion (ILE) have led to its potential application in the treatment of poisoning. The present study aims to investigate the potential role of ILE as an antidote for acute METH poisoning. Materials and Methods: Two groups of six male rats were treated by METH (45 mg/kg), intraperitoneally. Five to seven min later, they received an infusion of 18.6 ml/kg ILE 20% through the tail vein or normal saline (NS). Locomotor and behavioral activity was assessed at different time after METH administration. Body temperature and survival rates were also evaluated. Brain and internal organs were then removed for histological examination and TUNEL assay. Results: ILE therapy for METH poisoning in rats could prevent rats mortalities and returned the METH-induced hyperthermia to normal rates (Pacute toxicity,antidote,Intravenous lipid emulsion Methamphetaminehttp://ijbms.mums.ac.ir/article_8236.htmlhttp://ijbms.mums.ac.ir/article_8236_a245be4d86263ec798a011f36e9b1d93.pdfMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-387420220170201Effect of sodium hydrosulfide on mRNA expression of prostaglandin E2 receptors in response to mucosal acidification and distention-induced gastric acid secretion in rats145149ENSeyyed AliMardPhysiology Research Center and Research Center for Infectious Diseases of Digestive System, Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iranalimard77@gmail.com;mard-sa@ajums.ac.irSiminMahiniPhysiology Research Center and Research Center for Infectious Diseases of Digestive System, Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranMahinDianatPhysiology Research Center and Department. of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranYaghoobFarboodPhysiology Research Center and Department. of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iranfarbood-y@ajums.ac.ir10.22038/ijbms.2017.8237Objective(s): Prostaglandins have been shown to mediate the gastro-protective effect of sodium hydrosulfide (NaHS) but effect of NaHS on mRNA expression of prostaglandin E2 receptors (EP1, 3-4; EPs) has not been investigated. Therefore, this study designed to evaluate the effect of NaHS on mRNA expression of EPs receptors in response to mucosal acidification and distention-induced gastric acid secretion in rats. Materials and Methods: Fasted rats were randomly assigned into 4 groups (n=6/group). They were control, and NaHS-treated groups. To evaluate the effect of NaHS on mucosal mRNA expression of EPs receptors, the gastric mucosa exposed to stimulated gastric acid output and mucosal acidification. The pylorus sphincter catheterized for instillation of isotonic neutral saline or acidic solution. Ninety min after beginning the experiments, animals sacrificed and the gastric mucosa collected to determine the pH, mucus secretion and to quantify the mRNA expression of EPs receptors by quantitative real-time PCR. Results: present results showed that a) NaHS increased the mucus secretion, mRNA expression of EP3 and EP4 receptors in response to distention-induced expression; b) The mRNA expression of EP1 receptors increased while EP4 mRNA receptors decreased in response to mucosal acidification in NaHS-pretreated rats; and c) NaHS increased pH of gastric contents both in response to distention-induced gastric acid secretion and mucosal acidification. Conclusion: NaHS behaves in a different manner. It effectively only increased the pH of gastric contents to reinforce the gastric mucosa against a highly acidic solution but modulated both acid and mucus secretion when the rate of acid increase in the stomach was slower.Mucosal acidification,Prostaglandin E2 receptors,Rat,Sodium hydrosulfidehttp://ijbms.mums.ac.ir/article_8237.htmlhttp://ijbms.mums.ac.ir/article_8237_fb96cd37d12a6aefe98b357532adde52.pdfMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-387420220170201Gestational diabetes leads to down-regulation of CDK4-pRB-E2F1 pathway genes in pancreatic islets of rat offspring150154ENZahraNazariDepartment of Animal Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iranzahra_nazari@ajums.ac.irMohammadNabiuniDepartment of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Irannabiuni@tmu.ac.irMohsenSaeidiStem Cell Research Center, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iransaeedi.m٥٠@gmail.comMohammad JafarGolalipourGorgan Congenital Malformations Research Center, Depatment of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iranmjgolalipour@yahoo.com10.22038/ijbms.2017.8240Objective(s): The link between a hyperglycemic intrauterine environment and the development of diabetes later in life has been observed in offspring exposed to gestational diabetes mellitus (GDM), but the underlying mechanisms for this phenomenon are still not clear. Reduced β-cells mass is a determinant in the development of diabetes (type 1 and type 2 diabetes). Some recent studies have provided evidence that the CDK4-pRB-E2F1 regulatory pathway is involved in β-cells proliferation. Therefore, we postulated that GDM exposure impacts the offspring’s β-cells by disruption in the CDK4-pRB-E2F1 pathway. Materials and Methods: Adult Wistar rats were randomly allocated in control and diabetic group. The experimental group received 40 mg/kg/body weight of streptozotocin (STZ) on day zero of gestation. After delivery, diabetic offspring of GDM mothers and control dams at the age of 15 week were randomly scarified and pancreases were harvested. Langerhans islets of diabetic and control groups were digested by collagenase digestion technique. After RNA extraction, we investigated the expressions of the kir 6.2 and CDK4-pRB-E2F1 pathway genes by quantitative real-time PCR. Results: GDM reduced the expression of CDK4-pRB-E2F1 pathway genes in Langerhans islets cells of offspring. CDK4, pRB and E2F1 pathway genes were downregulated in diabetic islets by 51%, 35% and 84%, respectively. Also, the expression of Kir 6.2 was significantly decreased in diabetic islets by 88%. Conclusion: We suggest that the effect of gestational diabetes on offspring’s β-cells may be primarily caused by the suppression of CDK4-pRB-E2F1 pathway.Gene expression,Gestational diabetes mellitus,Langerhans islets,Offspringhttp://ijbms.mums.ac.ir/article_8240.htmlhttp://ijbms.mums.ac.ir/article_8240_645605c2fe3d88504726c0f3f1e04631.pdfMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-387420220170201The effect of hydro-ethanolic extract of Curcuma longa rhizome and curcumin on total and differential WBC and serum oxidant, antioxidant biomarkers in rat model of asthma155165ENFarzanehShakeriNeurogeneeic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iranshakerif911@mums.ac.irMohammadSoukhtanlooDepartment of Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iransoukhtanloo.m@mums.ac.irMohammad HosseinBoskabadyNeurogeneeic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iranboskabadymh@mums.ac.ir10.22038/ijbms.2017.8241Objective(s): The effects of Curcuma longa (C. longa) and curcumin on total and differential WBC count and oxidant, antioxidant biomarkers, in rat model of asthma were evaluated. Materials and Methods: Total and differential WBC count in the blood, NO2, NO3, MDA, SOD, CAT and thiol levels in serum were examined in control, asthma, Asthmatic rats treated with C. longa (0.75, 1.50, and 3.00 mg/ml), curcumin (0.15, 0.30, and 0.60 mg/ml), and dexamethasone (1.25 μg/ml) rats. Results: Total and most differential WBC count, NO2, NO3 and MDA were increased but lymphocytes, SOD, CAT and thiol were decreased in asthmatic animals compared to controls (PCurcuma longa,Curcumin,Inflammation,Oxidative Stress,Rat model of asthma,WBChttp://ijbms.mums.ac.ir/article_8241.htmlhttp://ijbms.mums.ac.ir/article_8241_11bd1c7611720d7389bfa457776e5aac.pdfMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-387420220170201Cytotoxic and apoptotic effects of different extracts of Artemisia biennis Willd. on K562 and HL-60 cell lines166171ENZahraTayarani-NajaranDepartment of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Irantayraninz@mums.ac.irFarideh-SadatMakkiDepartment of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran|Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranNafiseh-SadatAlamolhodaeiDepartment of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran|Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranMahdiMojarrabPharmaceutical Sciences Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iranmmojarrab@kums.ac.irSeyed AhmadEmamiBiotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iransaemami@mums.ac.ir10.22038/ijbms.2017.8242Objective(s): Artemisia is a genus of herbs and small shrubs forms an important part of natural vegetation in Iran. It has been reported that several Artemisia species possess anti-proliferative effects. Considering the value of this genus in anti-cancer researches we have chosen Artemisia biennis for cytotoxic and mechanistic studies. Materials and Methods:In this study we have investigated the cytotoxic and apoptotic effects of petroleum ether, dichloromethane, ethyl acetate, ethanol, and ethanol:water (1:1 v/v) extracts of A. biennis Willd. on two cancer human cell lines (K562 and HL-60) and J774 as normal cells. Results: CH2Cl2 extract was found to have the highest anti-proliferative effect on cancer cells. IC50 values obtained in AlamarBlue® assay for CH2Cl2 extract were 64.86 and 54.31 µg/ml on K562 and HL-60 cells respectively. In flow cytometry histogram of the cells treated with CH2Cl2 extract, sub-G1 peak was induced. DNA fragmentation, increased in the level of Bax and cleavage of PARP protein all showed the induction of apoptosis with CH2Cl2 extract after 48 hr contact with cells. Conclusion: The results can corroborate the cytotoxic and apoptotic effects of the CH2Cl2 extract of A. biennis on the K562 and HL-60 cancer cell lines.Artemisia biennis,Asteraceae,Apoptosis,Leukemic cell lineshttp://ijbms.mums.ac.ir/article_8242.htmlhttp://ijbms.mums.ac.ir/article_8242_6fc3454348e037bd1fc4d1942eaa0161.pdfMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-387420220170201Evaluation of the expression of VIII factor and VEGF in the regeneration of non-vital teeth in dogs using propolis172177ENMinaZareiDental Research Center, Department of Endodontics, School of Dentistry, Mashhad University of Medical Science, Mashhad, Iranzareim@mums.ac.irAmir HosseinJafarianPathology research Center, Mashhad University of Medical Science, Mashhad, Iranjafarian.a@chmail.irAzadehHarandiDepartment of Endodontics, School of Dentistry, Mazandaran University, Babol, Iranharandia@mums.irMaryamJavidiDental Research Center, Department of Endodontics, School of Dentistry, Mashhad University of Medical Science, Mashhad, Iranjavidim@mums.ac.irMaryamGharechahiDental Materials Research Center, Department of Endodontics, Mashhad Dental School, Mashhad University of Medical Science, Mashhad, Irangharechahim@mums.ac.ir10.22038/ijbms.2017.8243Objective(s): The purpose of the present study was the immunohistochemical evaluation of VEGF and VII factors in dog’s teeth pulp revascularized with MTA and propolis. Materials and Methods: 144 mature and immature two rooted dog’s premolar canals were selected. Pulp necrosis and infection were established after 2 weeks and the disinfection of the canals was done with copious NaOCl irrigation and triantibiotic mixture (ciprofloxacin, metronidazole, and minocycline) for 3 weeks. Subsequently, the blood clot was evoked in the canal by periapical tissue irritation with a k-file. The samples were randomly divided into 6 experimental groups: propolis (groups 1, 2), MTA (groups 3, 4), and parafilm (groups 5, 6) in immature and mature teeth. The animals were sacrificed and samples were prepared for immunohistochemical evaluation of VEGF and the VIII factor. Results: Tissue regeneration was seen in 64.5% of MTA, 38% of propolis, and 0% of parafilm group samples. Expression of VEGF and VIII factor in the propolis group was more than the MTA group and it showed a reduction after 3 months in comparison to 1 month. VEGF and VIII factor were seen in stromal cells in addition to endothelial vessel cells. Overall, expression of angiogenic factors was more in the open apex teeth compared to close apex ones. Conclusion: According to the results of this study, propolis can induce the expression of VEGF and VIII factor in infected mature and immature dog’s teeth and is a suitable biomaterial for the revascularization technique.Regeneration,Propolis,VIII Factor,VEGFhttp://ijbms.mums.ac.ir/article_8243.htmlhttp://ijbms.mums.ac.ir/article_8243_5940d9e6fa0ba922e8de498a646e3932.pdfMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-387420220170201Expression pattern of neurotrophins and their receptors during neuronal differentiation of adipose-derived stem cells in simulated microgravity condition178186ENVajihehZarrinpourDepartment of Biology, Fars Science and Research Branch, Islamic Azad University, Marvdasht, Iran|Department of Biology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iranzarinpour33@yahoo.comZahraHajebrahimiAerospace Research Institute, Ministry of Science Research and Technology, Tehran, Iranhajebrahimi@ari.ac.irMojtabaJafariniaDepartment of Biology, Fars Science and Research Branch, Islamic Azad University, Marvdasht, Iran|Department of Biology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iranjaafarinia@yahoo.com10.22038/ijbms.2017.8244Objective(s): Studies have confirmed that microgravity, as a mechanical factor, influences both differentiation and function of mesenchymal stem cells. Here we investigated the effects of simulated microgravity on neural differentiation of human adipose-derived stem cells (ADSCs).
Materials and Methods:We have used a fast rotating clinostat (clinorotation) to simulate microgravity condition. Real-time PCR and flow cytometry analysis were used to evaluate the regulation of neurotrophins, their receptors, and neural markers by simulated microgravity and their impact on neural differentiation of cells.
Results: Our data revealed that simulation microgravity up-regulated the expression of MAP-2, BDNF, TrkB, NT-3, and TrkC both before and after neural differentiation. Also, the neural cells derived from ADSCs in microgravity condition expressed more MAP-2, GFAP, and synaptophysin protein in comparison to the 1G control.
Conclusion: We showed that simulated microgravity can enhance the differentiation of mesenchymal stem cells into neurons. Our findings provide a new strategy for differentiation of ADSCs to neural-like cells and probably other cell lineages. Meanwhile, microgravity simulation had no adverse effects on the viability of the cells and could be used as a new environment to successfully manipulate cells.ADSCs,Microgravity,Neural differentiation Neurotrophinhttp://ijbms.mums.ac.ir/article_8244.htmlhttp://ijbms.mums.ac.ir/article_8244_927397fdb33e1bb1f08fe43e81828f9b.pdfMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-387420220170201The miR-383-LDHA axis regulates cell proliferation, invasion and glycolysis in hepatocellular cancer187192ENZhixiongFangDepartment of Infectious Disease, XiangTan City Central Hospital, XiangTan, People's Republic of Chinafzx8214907@sina.comLangqiuHeDepartment of Infectious Disease, XiangTan City Central Hospital, XiangTan, People's Republic of ChinaHuiJiaDepartment of Infectious Disease, XiangTan City Central Hospital, XiangTan, People's Republic of China501892258@qq.comQiushengHuangDepartment of Infectious Disease, XiangTan City Central Hospital, XiangTan, People's Republic of Chinahzwwhs@126.comDanChenEdong Healthcare City Hospital of Traditional Chinese Medicine, Inefections Disease Hospital, Huangshi, People's Republic of Chinachen9980@163.comZhiweiZhangCancer Research Institute, University of South China, Key Laboratory of Cancer Cellular and Molecular Pathology of Hunan Provincial University, Hengyang, People's Republic of Chinanhdxzzw@qq.com10.22038/ijbms.2017.8246Objective(s): To explore the correlation between expression patterns and functions of miR-383 and LDHA in hepatocellular cancer (HCC). Materials and Methods: We detected the expression of miR-383 and LDHA in 30 HCC tissues and their matched adjacent normal tissues using qRT-PCR. Then we performed MTT assay, foci formation assay, transwell migration assay, glucose uptake assay and lactate production assay to explore the function of miR-383 in cell proliferation, invasion and glycolysis in HCC cell lines. Luciferase reporter assay was used to explore whether LDHA was a target gene of miR-383. Western blot and qRT-PCR were used to further confirm LDHA was targeted by miR-383. Then the above functional experiments were repeated to see whether the function of LDHA could be inhibited by miR-383. Results: The results of qRT-PCR showed that miR-383 was down-regulated in HCC tissues compared with their matched adjacent normal tissues. Functional experiments showed that overexpression of miR-383 significantly suppressed cell proliferation, invasion and glycolysis. Luciferase reporter assay showed LDHA was a target gene of miR-383 and expression of LDHA was inversely correlated with that of miR-383 in HCC. Besides, increased cell proliferation, invasion and glycolysis triggered by LDHA could be inhibited by overexpression of miR-383 in HCC cell lines. Conclusion: Our study proved that miR-383 is down-regulated in HCC and acts as a tumor suppressor through targeting LDHA. Targeting the miR-383-LDHA axis might be a promising strategy in HCC treatment.Hepatocellular cancer,LDHA,MiR-383http://ijbms.mums.ac.ir/article_8246.htmlhttp://ijbms.mums.ac.ir/article_8246_f9d7ede7fec701e73da750ce888c7cdd.pdfMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-387420220170201In vitro lymphoproliferative response and cytokine production in mice with experimental disseminated candidiasis193198ENAli RezaKhosraviMycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Irankhosravi@ut.ac.irHojjatollahShokriDepartment of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iranhshokri@umz.ac.irShahinEshghiDepartment of Food Sciences, Qazvin, Iranian Veterinary Organizationeshghis@gmail.com10.22038/ijbms.2017.8248Objective(s): Systemic candidiasis is an infection of Candida albicans (C. albicans) causing disseminated disease and sepsis, invariably when host defenses are compromised. We investigated the histopathological changes as well as the lymphoproliferative responses and cytokine production of splenic cells after stimulation with Concanavalin A (Con A) and Pokeweed mitogen (PWM) in mice with disseminated candidiasis. Materials and Methods:Lymphoproliferative responses were stimulated in vitro with Con A (1 µg/ml) and PWM (1 µg/ml) mitogens in Roswell Park Memorial Institute (RPMI) 1640 media, and the production of interferon (IFN)-γ and interleukin-4 (IL-4) in the supernatants was measured by enzyme-linked immunosorbent assay (ELISA). Results: The results revealed that C. albicans organisms multiplied to a greater extent in the kidneys than in the liver and spleen of infected mice. The most predominant forms of C. albicans in different parts of the kidneys were yeast mixed with hyphal forms. Infected mice had a significantly increased proliferative response when splenocytes were stimulated with PWM (2.0±0.16) and Con A (1.9±0.19) (PCandida albicans,Cytokine production,Lymphocyte proliferation,Mitogens,Mice,Systemic candidiasishttp://ijbms.mums.ac.ir/article_8248.htmlhttp://ijbms.mums.ac.ir/article_8248_bc3978618ad156c11d070f423090e845.pdfMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-387420220170201Nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression199208ENMaryamMosadeghDepartment of Comparative Histology & Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iranmosadegmaryam@gmail.comShapourHasanzadehDepartment of Comparative Histology & Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, Irans.hasanzadeh@mail.urmia.ac.irMazdakRaziDepartment of Comparative Histology & Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iranmazdak.razi@gmail.com10.22038/ijbms.2017.8249Objective(s): Present study was performed in order to uncover new aspects for nicotine-induced damages on spermatogenesis cell lineage. Materials and Methods: For this purpose, 36 mature male Wistar rats were divided into three groups as; control-sham (0.2 ml, saline normal, IP), low dose (0.2 mg/kg BW-1, IP) nicotine-received and high dose (0.4 mg/kg BW-1, IP) nicotine-received groups. Following 7 weeks, the expression of bcl-2, p53 and caspase-3 at mRNA and protein levels were investigated by using reverse-transcriptase PCR (RT-PCR) and immunohistochemical (IHC) analyses, respectively. Moreover, the serum level of FSH, LH and testosterone were evaluated. Finally, the mRNA damage was analyzed by using special fluorescent staining. Results: Nicotine, at both dose levels, decreased tubular differentiation, spermiogenesis and repopulation indices and enhanced cellular depletion. Animals in nicotine-received groups exhibited a significant (PApoptosis,Bcl-2,Caspase-3,Nicotine,P53,Testis,Testosteronehttp://ijbms.mums.ac.ir/article_8249.htmlhttp://ijbms.mums.ac.ir/article_8249_5350c5d915b8ac76c832531572cb9331.pdfMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-387420220170201Up-regulation of TLR2 and TLR4 in high mobility group Box1-stimulated macrophages in pulpitis patients209215ENJavadMahmoudiNeurosciences Research Center, Tabriz University of Medical Science, Tabriz, Iranjavad_mahmodi@yahoo.comBabakSabermaroufNeurosciences Research Center, Tabriz University of Medical Science, Tabriz, Iranbabak_s@gmail.comBehzadBaradaranImmunology Research Center, Tabriz University of Medical Science, Tabriz, Iranbehzad_m@yahoo.comLeilaSadat-HatamnezhadImmunology Research Center, Tabriz University of Medical Science, Tabriz, IranSiamakSandoghchian ShotorbaniDepartment of Immunology, Tabriz Branch, Islamic Azad University, Tabriz, Irandr.sandoghchian@iaut.ac.ir10.22038/ijbms.2017.8250Objective(s): High Mobility Group Box1 (HMGB1) is a nonhistone, DNA-binding protein that serves a crucial role in regulating gene transcription and is involved in a variety of proinflammatory, extracellular activities. The aim of this study was to explore whether HMGB1 stimulation can up-regulate the expression of Toll-like Receptor 2 (TLR2) and Toll-like Receptor 4 (TLR4) on macrophages from pulpitis and to clarify the subsequent events involving Th17 cells and Th17 cell-associated cytokine changes. Materials and Methods: Having prepared dental pulp tissues of pulpitis and healthy controls, macrophage were isolated and cultured. Macrophages were thereafter stimulated by HMGB1 time course. RT-QPCR, flowcytometer, immunofluorescence, Western blotting, and ELISA techniques were used in the present research. Results: Our results showed that the expression of TLR2 and TLR4 on macrophages stimulated with HMGB1 increased in pulpitis compared with controls (macrophages without HMGB1 stimulation) with a statistical signiﬁcance (PHMGB1,Pulpitis,TLR2,TLR4http://ijbms.mums.ac.ir/article_8250.htmlhttp://ijbms.mums.ac.ir/article_8250_3b98ce9154b9aecf3ce2d86fb6b01e14.pdfMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-387420220170201Gestational diabetes influences retinal Muller cells in rat's offspring216221ENAkramsadatTabasiDepartment of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, IranSorayaGhafariGorgan Congenital Malformations Research Center, Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, IranMehdiMehdizadehDepartment of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iranmaranaoo2004@yahoo.comMajidAsadi ShekariNeuroscience Research Center, Neuropharmacology Institute. Kerman University of Medical Sciences, Kerman, Iranmajidasa@gmail.comMohammad JafarGolalipourGorgan Congenital Malformations Research Center, Department of Anatomical Sciences, Golestan University of Medical Sciences, Gorgan, Iranmjgolalipour@yahoo.com10.22038/ijbms.2017.8251Objective(s): The Muller cell is the principal glial cell of the vertebrate retina. The expression of Glial fibrillary acidic protein (GFAP) in the Muller cells was used as a cellular marker for retinal damage. This study was done to evaluate the effect of gestational diabetes on retinal Muller cells in rat's offspring. Materials and Methods: In this experimental study, 12 Wistar rat dams were randomly allocated in control and diabetic groups. Gestational diabetes was induced by 40 mg/kg/body weight of streptozotocin at the first day of gestation, intraperitoneally. Dams in control group received an equivalent volume normal saline. Eye of six offspring of each group were removed at postnatal day 28 (P28). The histopathological changes in retina were examined through H&E staining and ultrastructure transmission electron microscopy (TEM). The expression of GFAP was examined using Immunohisto-chemical staining of GFAP in Muller cells. Photographs of retina were taken using Olympus BX51 microscope and a digital camera DP12 and EM LEO906; Zeiss, Germany. Results: In the control rat's offspring, GFAP expression was not significant in Muller cells. According to the optical microscope images, GFAP expression was observed in the processes of the Muller cell in the inner plexiform layer of retina in offspring of diabetic mothers. In TEM technique, nuclear fragmentation and apoptotic bodies were observed in Muller cell of diabetic offspring. Conclusion: This study showed that the uncontrolled gestational diabetes can increase GFAP expression in Muller cells and retinal thickness of retinal layer in rat offspring's, therefore uncontrolled gestational can damage the Muller cells.Gestational diabetes,GFAP,Mellitus,Muller cell,Retinahttp://ijbms.mums.ac.ir/article_8251.htmlhttp://ijbms.mums.ac.ir/article_8251_fbe5c09673ea26e4c2ca17814d1e4c85.pdf