During assaying an enzyme at high temperature, the substrate (Pectin) is degraded by the high temperature rather than by enzyme, so, how can I minimize degradation of the substrate by the temperature? ...

Does the membrane potential usually quoted for gram negative bacteria (e.g. E. coli) refer to the potential across both membranes? - If yes, then does the potential fall more over the inner or outer ...

I am told that beta branching interferes with alpha helix formation.
Problem is that I don't see how beta branching has anything to do with alpha-helix formation. Beta-branches are on the outside of ...

I recall reading that much of the energy a cat produces from its food comes from proteins which I assume would produce energy via being catabolised into amino acids which in turn, if glucogenic, would ...

I would like to know if there is an assay which could allow us to analyse a protein before it has assumed its 3D functional form.
While studying structural biology, I only came to know the forces that ...

I understand that the human body when performing Fatty Acid Synthesis can synthesize only until C16 (palmitate). However the ER has desaturases and elongases. I know that desaturases are used to add ...

What happens when someone takes an anti-venom for an animal they weren't bit by, either for incorrect identification of the animal they were bit by or if they weren't bit at all?
I am looking for the ...

What tests could be run to test the purity and type of HCG?
We are looking to purchase HCG from China but the purity and quality varies between labs, we are able to receive samples of the raw peptide ...

I have heard that omega-3 fatty acids found in fish are a good way to prevent/reduce cholesterol problems.
My question is how do omega-3 fatty acids do that: what are they doing on a chemical level ...

Does anyone know where I can find a reliable list of virucides (not anti-virus) that can be used by humans? This virucide must be able to affect viruses that are already inside the cells. St John's ...

I quantified my protein using the standard Bradford method and the A280 methods and obtained values that were far off from the theoretical value of the protein of interest, and therefore was wondering ...

I'm a freshman med student and I've noticed that on all the feet (and hands) of deceased people that I've worked with, the bottom of the feet always turns red and also the inside of the palm? Why is ...

Someone claimed that the dinosaurs could only live because back then athmospheric density was around 650 kg/m³, providing some buyoncy, this claim is checked on Skeptics SE. The claim itself is beside ...

I have run across an interesting case that is similar to only two others I've encountered. What makes it interesting is the combination of undetectable (under normal testing conditions, can elaborate ...

I have been thinking about some alternative CO₂ fixation pathways. As almost all of them include ferredoxin reduction I started to wonder where does the ferredoxin comes from. I could not find any of ...

At least one of the journal articles that reported on such clinical trials allured to the nicotinamide-tryptophan pathway that I assume must be related to the metabolism of tryptophan into serotonin ...

Let's say a Lysosome that bonds to a hazardous material to neutralize it has all bonding sites used up. would it be more safe / efficient to excrete the entire Lysosome / hazardous material molecule ...

In the body we almost always assume that glutamate exists as glutamate rather than glutamic acid. It is so commonly glutamate yet glutamic acid and glutamate share the abbreviations of Glu and E. From ...