Equivalent Testing Methodology for Agricultural Water

FDA has determined that the following quantification methods are scientifically valid and at least equivalent to the method of analysis in § 112.151(a), “Method 1603: Escherichia coli (E. coli) in Water by Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar (Modified mTEC)” (December 2009), in accuracy, precision, and sensitivity in quantifying generic Escherichia coli in agricultural water when used in connection with the criteria described in § 112.44(a) or § 112.44(b).

IDEXX Colilert Test Kit, but only if using IDEXX Quanti-Tray/2000 for quantification.

IDEXX Colilert-18 Test Kit, but only if using IDEXX Quanti-Tray/2000 for quantification.

With regard to criteria described only in § 112.44(a), FDA has determined that the following presence/absence methods are scientifically valid and at least equivalent to the method of analysis in § 112.151(a), “Method 1603: Escherichia coli (E. coli) in Water by Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar (Modified mTEC)” (December 2009), in accuracy, precision, and sensitivity in detecting generic Escherichia coli in agricultural water.

TECTATM EC/TC medium and the TECTATM Instrument: A Presence/Absence Method for the Simultaneous Detection of Total Coliforms and Escherichia coli (E. coli) in Drinking Water. (2014).

Modified ColitagTM Test Method for the Simultaneous Detection of E. coli and other Total Coliforms in Water. ATP D05-0035. (2009).