BIONIX Low Oxygen Culture Kit

No need to use gas cylinder of carbon dioxide, nitrogen gas and mixed gas.

Enable to control O2 concentration between 0.1-15% for cultivation

Cancer cell cultivation requires a low oxygen environment. Previously a special CO2 incubator and gas cylinder of carbon dioxide, nitrogen gas and mixed gas were necessary to create this environment. Despite initial costs and running cost being high for creating this environment with them, it only reached a 0.5% low oxygen environment, most of the time it reached about 2% low oxygen environment that it was unable to maintain.

Procedure

Place 《O2 meter》 deep inside of the《Gas barrier pouch bag》and place the petri dish filled with culture solution right next to it.

Open the aluminum bag and take out《Gas concentration regulator》. Place it close to Line A. *Please refer to the picture below.

Close Line B with 《Clip》. O2 inside of the pouch will be absorbed in about 10 minutes and provides a low oxygen environment.

Keep eyes on O2 meter. When it reaches its desired O2 concentration, exchange the air inside of the petri dish with the air inside of the pouch. Wait until O2 concentration lowered to a little less than the desired concentration, and clip on Line A and stop the O2 absorption.

If the O2 concentration is much higher or lower than the desired concentration, it requires some adjustment process. Please inquire.

BIONIX uses a gas concentration regulator called Anaeropack-kenki. Many articles of the cell ischemic experiments are published using Anaeropack-kenki 5%. Please search for these articles using the keywords Bionix and hypoxia.