A simple and fast electrophoretic method for elution of nucleic acids from gels.

MedLine Citation:

PMID:
530273
Owner:
NLM
Status:
MEDLINE

Abstract/OtherAbstract:

A very convenient electrophoretic procedure for DNA or RNA elution from agarose or polyacrylamide gels is described. The gel piece with nucleic acid to be eluted is contained in a dialysis bag filled with buffer and elution is carried out in a horizontal electrophoresis apparatus. The nucleic acid is recovered with a high yield and can be used, without prior treatment, in further enzymatic or chemical reactions. Results obtained with DNA are presented here.