Bottom Line:
Human bocavirus (HBoV) and HBoV2, two human bocavirus species, were found in 18 and 10 of 235 nasopharyngeal aspirates, respectively, from children hospitalized with acute respiratory tract infection.Our results suggest that, like HBoV, HBoV2 is distributed worldwide and may be associated with respiratory and enteric diseases.

ABSTRACTHuman bocavirus (HBoV) and HBoV2, two human bocavirus species, were found in 18 and 10 of 235 nasopharyngeal aspirates, respectively, from children hospitalized with acute respiratory tract infection. Our results suggest that, like HBoV, HBoV2 is distributed worldwide and may be associated with respiratory and enteric diseases.

Mentions:
In total, 260 viruses were identified in 196 (83.4%) of the 235 children. Using nested PCR, we found 21 positive specimens; further nucleotide sequence analysis showed that 10 (4.3%) were HBoV2 and 11 were HBoV (Figure, panel A). All 11 HBoV strains detected by using HBoV2 nested-PCR were included in the 18 HBoV-positive patients as determined by PCR using primers 188F and 542R. Of the 10 HBoV2-positive patients, 7 (70%) were co-infected with other respiratory viruses, including 4 patients with RSV. Of the 18 HBoV-positive patients, 12 (66.7%) displayed co-infections. There were no statistically significant differences in the HBoV2 and HBoV detection (p = 0.119 by χ2 test) and co-infection (p = 1.000 by Fisher exact test) rates.

Mentions:
In total, 260 viruses were identified in 196 (83.4%) of the 235 children. Using nested PCR, we found 21 positive specimens; further nucleotide sequence analysis showed that 10 (4.3%) were HBoV2 and 11 were HBoV (Figure, panel A). All 11 HBoV strains detected by using HBoV2 nested-PCR were included in the 18 HBoV-positive patients as determined by PCR using primers 188F and 542R. Of the 10 HBoV2-positive patients, 7 (70%) were co-infected with other respiratory viruses, including 4 patients with RSV. Of the 18 HBoV-positive patients, 12 (66.7%) displayed co-infections. There were no statistically significant differences in the HBoV2 and HBoV detection (p = 0.119 by χ2 test) and co-infection (p = 1.000 by Fisher exact test) rates.

Bottom Line:
Human bocavirus (HBoV) and HBoV2, two human bocavirus species, were found in 18 and 10 of 235 nasopharyngeal aspirates, respectively, from children hospitalized with acute respiratory tract infection.Our results suggest that, like HBoV, HBoV2 is distributed worldwide and may be associated with respiratory and enteric diseases.

ABSTRACTHuman bocavirus (HBoV) and HBoV2, two human bocavirus species, were found in 18 and 10 of 235 nasopharyngeal aspirates, respectively, from children hospitalized with acute respiratory tract infection. Our results suggest that, like HBoV, HBoV2 is distributed worldwide and may be associated with respiratory and enteric diseases.