GL-Tip SCX and GL-Tip SDB-SCX

Spin tips for peptide fractionation

GL-Tip SCX is packed with strong cation polymer (SCX) and GL-Tip SDB-SCX are packed with styrene divinylbenzene polymer (SDB) and strong cation polymer (SCX). GL-Tip SDB-SCX is packed in a two layer format consisting an SDB and SCX media. Undesalted peptide samples can be used in GL-Tip SDB-SCX as the first SDB layer can desalt the sample.

Sample loading capacity

Number of Quantified Peptides using GL-Tip SCX

Compared results between commercially available brand G’s tip column. GL-Tip SCX recovered more peptides. The usage of the newly developed TFA gradient elution method provide less chance of identifying the same peptide from one fractionated sample peptide to another resulting in higher efficiency.