Molecular studies of the role of the negative transcription elongation factor Nelf-E in Drosophila development using RNA interference

dc.date.accessioned

2013-03-12T08:39:23Z

dc.date.available

2013-03-12T08:39:23Z

dc.date.issued

2006

en_US

dc.date.submitted

2006-11-14

en_US

dc.identifier.citation

Athanasiv, Lavinia. Molecular studies of the role of the negative transcription elongation factor Nelf-E in Drosophila development using RNA interference. Masteroppgave, University of Oslo, 2006

en_US

dc.identifier.uri

http://hdl.handle.net/10852/11509

dc.description.abstract

The elongation step of transcription is now recognized as a critical target for transcription regulation. An increasing number of elongation factors have been identified, and the regulatory mechanism of elongation seems to be as complex as that of transcription initiation. A multitude of factors interact and regulate each other to mediate the exquisite regulation of transcription in response to biological processes. Promoter proximal pausing of the RNA polymerase II was first discovered on the hsp70 gene, but has also been documented on estrogen stimulated genes. It is suggested that NELF functions as a control point for proper mRNA capping.
Here we describe the characterization of Drosophila Nelf-E, one of the subunits of the Negative transcription elongation factor complex. Functional analyses were performed to assess the role of Nelf-E during Drosophila development. RT-PCR on Nelf-E knock-down flies showed an up-regulation of integrin and integrin-associated proteins.
Further analyses are needed to investigate the functional implications of the NELF complex, and to authenticate the target gene of this transcription elongation repressor.

nor

dc.language.iso

eng

en_US

dc.subject

livssyklus Drosophila bananflue vinge utvikling insekter integriner

en_US

dc.title

Molecular studies of the role of the negative transcription elongation factor Nelf-E in Drosophila development using RNA interference

en_US

dc.type

Master thesis

en_US

dc.date.updated

2006-12-15

en_US

dc.creator.author

Athanasiv, Lavinia

en_US

dc.subject.nsi

VDP::473

en_US

dc.identifier.bibliographiccitation

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