Challenges of Quantitative High Throughput Confocal Microscopy of 3D Spheroids

Multicellular 3D spheroids are providing far more biological complexity than standard 2D monolayer cell culture. There is growing excitement for the use of spheroids in phenotypic drug discovery and the development of more predictive models of toxicity. However, unlike thin 2D monolayers that are easily imaged, there are significant challenges to quantitative confocal imaging of spheroids. This talk will address these challenges as they pertain to quantitative high throughput confocal microscopy.

Jeffrey Morgan

Professor of Medical Science and EngineeringBrown University

Jeff Morgan is Professor of Medical Science and Engineering at Brown University. A graduate of Syracuse (BS) and Harvard (PhD) with postdoctoral training at MIT and the Whitehead Institute, he was on the faculty of Harvard Medical School prior to Brown. Dr. Morgan has received numerous awards and patents for his research in gene therapy and tissue engineering and he has co-founded three companies including his latest, Microtissues, Inc (www.microtissues.com). Among his inventions are the 3D Petri Dish®, a technology for growing cells in three dimensions with applications in toxicity testing, drug discovery and tissue engineering. Dr. Morgan has published over 130 peer-reviewed papers, reviews and chapters and has edited two books and is an inventor of 12 U.S. and international patents. He is a Fellow of the National Academy of Inventors as well as a Fellow of the American Institute of Medical and Biological Engineering.