Hi,
I want to visualise sea pansy (renilla) luciferase enzyme/protein produced from the in-vitro coupled wheat germ system from Promega. Here are my options:
Option 1. AUTORADIOGRAPHY of radiolabelled luciferase [s35 Met] - lab cannot work with radioactive material, so this is ruled out.
Option 2. LUMINOMETER to detect luciferase luminescence - luminometer is not functional at the moment, so this is ruled out.
Option 3. WESTERN BLOT using antibody against luciferase - this is a viable option.
Option 4. Visualisation of luciferase luminescence by eye/microscope in a dark room - this is a viable option and quickest. Not sure how much protein (pg,ng,ug,mg??) you need before you can see some light glowing in the dark??
Cheers,
ST