Shared Facilities

Transgenic & Knock-Out Mouse

Policies & Procedures

Transgenic & Knock-Out Mouse Core service charges are as follows. See below for transgenic and knock-out mouse production procedures.

Transgenic & Knock-Out Mouse Core Service Charges

(Effective July 2014)

Service

Pricing

Pronuclear injection

$3,562

ES transfection

$6,861

Blast injection

$8,090

Single cell line binj

$3,549

Assisted reproduction

Sperm freeze

$628

IVF

$1,310

Embryo freeze

$764

Sperm cryo and verifi

$1,638

Thaw and transfer

$655

Cryo storage

$55/line/yr.

Core Policy Changes

The transfection charge is per transfection. A second transfection is a second charge.

Alternative strains can be used for pronuclear or blast injections, but the user must pay all additional mouse costs, including per diems while waiting for results.

If no founders are found from the first round of pronuclear injections, the user checks the DNA prep, then reinjection is done at no charge. If no founders are found from second round of injections, the user is charged for any additional injections.

Procedures

For transgenic mouse production, investigators provide the DNA construct to the core. The core then:

Harvests single cell embryos

Microinjects the embryos with the DNA construct

Transplants the microinjected embryos into pseudo-pregnant females

Performs Cesarean sections as required

Provides the resulting pups at weaning age to the investigator for analyses

For knock-out mouse production, the core provides three types of services:

For ES cell transfection, the investigator provides the core with a targeting vector. The core then:

transfects ES cells with the targeting vector

selects the resulting transfected clones

generates frozen stocks of the clones

provides the investigator with DNA samples from each of the clones for molecular analyses