I have a problem in assay calculation. where I analyse hard gelatin capsule of Amoxicillin. the capsule contains 266 mg of Amoxicillin Trihydrate equivlaent to 250mg of amoxicillin. I use the following equation. mg/cap = A t x W s( mg) x Dfu x P x Av. Fill Wt. of cap. A s x Dfs x W t (mg) x 100 where A t : the response of test solution.A s : the response of standard solution.Dfu : the dilution factor of test solution.Dfs : the dilution factor of standard solution.W t & W s : the weigh of Test and standard respectively.P : is the purity of amoxicillin standard.( here is the problem)where I have two assay of Amoxicillin working standard one is AS SUCH ( 85.9%) and ON DRY BASIS 98.8%) my question here when I use ON DRY BASIS potency should I have the result of mg/cap as amoxicillin base or WHAT???Provided that the USP does not recommend drying the standard of Amoxicillin trihydrate).Please can you help

As we have to find the content of amoxicillin trihydrate equivalent to Amoxicillin in mg per capsule of av.net.weight we have to use as such ie 85.9% for standard purity in calculation because rest of the percentage ,ie 14% may be of water molecule(as the R.S amoxicillin in its trihydrate form)

Potency is always As such unless and otherwise specified. Potency on dried basis should not be considered. since You are getting the area of amoxicillin but not for LOD/WATER by HPLC. you have the absorbance for 85.9% potency.

If you are going to report your potency report on basis of Iu/mg you take 85.9% and if you want to report in IU/cap you take according to that be cause youar measuring replative potency which is coimng according to your standrd which you take for the estimation.

I HAVE A PROBLEM IN POTENCY CALCULATION, difference between assay on dried basis, assay on as is basis and assay on anhydrous basis?
when we can calculate ssay on dried basis, assay on as is basis and assay on anhydrous basis?

I HAVE A PROBLEM IN POTENCY CALCULATION, difference between assay on dried basis, assay on as is basis and assay on anhydrous basis?
when we can calculate ssay on dried basis, assay on as is basis and assay on anhydrous basis?

pls help me to have calculation method and formula and sampl of calculateion

thanks alot

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