This assay has high sensitivity and excellent specificity for detection of Human OFQ/N. No significant cross-reactivity or interference between Human OFQ/N and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human OFQ/N and all the analogues, therefore, cross reaction may still exist.

Samples

Serum, Plasma, Biological Fluids

Assay Type

Sandwich

Detection Range

4.688--300pg/ml

Sensitivity

2.813pg/ml

Preparation and Storage

Store at 4 degree C.

Product Note

Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Other Notes

Small volumes of OFQ/N elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OFQ/N purchase

MBS2513211 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the OFQ/N, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 4.688--300pg/ml in biological research samples containing OFQ/N, with an estimated sensitivity of 2.813pg/ml. The ELISA analytical biochemical technique of the MBS2513211 kit is based on OFQ/N antibody-OFQ/N antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OFQ/N antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OFQ/N. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of OFQ/N in human serum, plasma and other biological fluidsPrinciple of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OFQ/N. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human OFQ/N and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OFQ/N, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human OFQ/N. You can calculate the concentration of Human OFQ/N in the samples by comparing the OD of the samples to the standard curve.

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Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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