U with macron is used in the alphabet of the Tajik language, where it represents the close-mid central rounded vowel, /ɵ/. Accordingly, although the letter shape is a modification of ⟨У⟩, the only aspect of the sound shared with /u/ is that both are relatively close, rounded vowels.

U with macron is used to represent long /u/ in Kildin Sami and Mansi, two Uralic languages spoken on the Kola peninsula (Kildin) and Western Siberia (Mansi). In these languages, length is distinctive, and macron is used to mark the long version of all the vowels.