Abstract

Background

The mimotopes of viruses are considered as the good targets for vaccine design. We
prepared mimotopes against multiple subtypes of influenza A and evaluate their immune
responses in flu virus challenged Balb/c mice.

Methods

The mimotopes of influenza A including pandemic H1N1, H3N2, H2N2 and H1N1 swine-origin
influenza virus were screened by peptide phage display libraries, respectively. These
mimotopes were engineered in one protein as multi- epitopes in Escherichia coli (E.
coli) and purified. Balb/c mice were immunized using the multi-mimotopes protein and
specific antibody responses were analyzed using hemagglutination inhibition (HI) assay
and enzyme-linked immunosorbent assay (ELISA). The lung inflammation level was evaluated
by hematoxylin and eosin (HE).