[Histonet] help with tumor ID

From:

"Malcolm McCallum"

My email was down for the four days after I sent this, so I figured I'ld send again! I had some students doing skeletochronology on frog legs. I have a strange section that appears to be possible hyperplasia of the periosteum. It apears to be a proliferation of poorly-staining connective tissue (H-E stain). Not sure though. Anyone out there willing to take a look at a jpg and tell me what they think?
VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org
A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation
and the World Congress of Herpetology.
Malcolm L. McCallum
Assistant Professor
Department of Biological Sciences
Texas A&M University Texarkana
2600 Robison Rd.
Texarkana, TX 75501
O: 1-903-223-3134
H: 1-903-791-3843
Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html
Spring Teaching Schedule & Office Hours:
Monday:
Genetics 1-2:40 pm
Office Hours 4-6 pm
Landscape Ecology 6-9:40 pm
Tuesday
Ichthyology 10-11:40 pm
Office Hours/Student Research 1-2:30 pm
Seminar 2:30-3:30 pm
Wednesday
Genetics 1-2:40 pm
Office Hours/Student Research 3-5 pm
Thursday
Ichthyology 10-11:40 pm
Office Hours/Student Research 1-4:30 pm
________________________________
From: histonet-bounces@lists.utsouthwestern.edu on behalf of Malcolm McCallum
Sent: Wed 2/21/2007 7:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] help with tumor ID
Hi,
I had some students doing skeletochronology on frog legs. I have a strange section that appears to be possible hyperplasia of the periosteum. It apears to be a proliferation of poorly-staining connective tissue (H-E stain). Not sure though. Anyone out there willing to take a look at a jpg and tell me what they think?
VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org
A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation
and the World Congress of Herpetology.
Malcolm L. McCallum
Assistant Professor
Department of Biological Sciences
Texas A&M University Texarkana
2600 Robison Rd.
Texarkana, TX 75501
O: 1-903-223-3134
H: 1-903-791-3843
Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html
Spring Teaching Schedule & Office Hours:
Monday:
Genetics 1-2:40 pm
Office Hours 4-6 pm
Landscape Ecology 6-9:40 pm
Tuesday
Ichthyology 10-11:40 pm
Office Hours/Student Research 1-2:30 pm
Seminar 2:30-3:30 pm
Wednesday
Genetics 1-2:40 pm
Office Hours/Student Research 3-5 pm
Thursday
Ichthyology 10-11:40 pm
Office Hours/Student Research 1-4:30 pm
________________________________
From: histonet-bounces@lists.utsouthwestern.edu on behalf of Smith, Allen
Sent: Wed 2/21/2007 7:18 PM
To: MVaughan4@ucok.edu
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Peripheral nerve stain
Kiernan's "physical developer method" for axons (J.A. Kiernan HISTOLOGICAL
AND HISTOCHEMICAL METHODS, 3rd ed., 1999, pp. 371-373)is the most sensitive
stain that I know of. It will beautifully demonstrate tiny nerve endings
that the Holmes method misses and the Winkelmann method just barely stains.
Expect it to take several tries until you get the timing of it just right
for your tissue.
Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida 33161
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
MVaughan4@ucok.edu
Sent: Wednesday, February 21, 2007 6:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Peripheral nerve stain
Histonetters,
I would like to stain a skin section to view nerve fibers, endings or
corpuscles.
Are there any specific stains that will pick up these structures in
paraffin embedded, formalin-fixed tissues?
One stain I have seen listed is erythrosin B and methylene blue, but I
haven't seen a protocol for this stain.
Any others? Thanks.
Mel
Melville B. Vaughan, Ph. D.
Assistant Professor
Department of Biology
University of Central Oklahoma
100 N. University Drive
Edmond, OK 73034
http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm
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