(Anglais) Conjugated polyelectrolytes combine the optoelectronic properties of
organic semiconductors and the physicochemical properties of
polyelectrolytes, such as water solubility. They have proven effective for
various potential applications, such as optoelectronic devices and the
detection of biomolecules[1, 2]. In continuation of our previous studies [3,
4], we here report a study on self-assemblies formed by DNA and a
conjugated polyelectrolyte, cationic polythiophene (CPT, see below), in
aqueous solution. A combined approach involving (chir)optical spectroscopy
and molecular modeling was used to get a better insight into the
supramolecular organization of DNA/CPT assemblies. We show that their
optical properties are dependent on the DNA sequence. Furthermore, we
show that DNA/CPT assemblies can be used for DNA hybridization sensors,
by detecting variations at the level of a single nucleotide.
References
1. Liu, B. and G.C. Bazan, Conjugated Polyelectrolytes: Fundamentals and
Applications. 2013: Wiley.
2. Jiang, H., et al., Angewandte Chemie International Edition, 2009. 48(24):
p. 4300-4316.
3. Rubio-Magnieto, J., et al., Chemical Communications, 2013. 49(48): p.
5483-5485.
4. Rubio-Magnieto, J., et al., Soft Matter, 2015. 11(32): p. 6460-6471.