Presenter Information

Location

Start Date

End Date

Student's Major

Student's College

Science, Engineering and Technology

Mentor's Name

Danae Quirk Dorr

Mentor's Department

Chemistry and Geology

Mentor's College

Science, Engineering and Technology

Description

Chemical reactions from biological and synthetic factors often form adducts with DNA. According to K.A. Naidu, decanal and trans-2-decenal have been touted to potentially possess anti-cancer properties. The purpose of this research was to explore whether either of these aldehydes lead to the formation of adducts with DNA. These aldehydes were dissolved in deuterated dimethyl sulfoxide (d6-DMSO) with and without the presence of arginine and treated with the nucleotide bases 2’- deoxyadenosine and 2-deoxyguanosine. After heating, precipitate was removed by filtration and reaction mixtures were analyzed using 1HNMR and HPLC. Decanal and trans-2-decenal were also allowed to treat calf thymus DNA. This reaction was heated at 50°C. Following acid hydrolysis, the reaction mixture was also analyzed by HPLC. Identifying and further characterizing adducts that form will ultimately allow further research into the chemo preventive or carcinogenic properties of these aldehydes.

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Apr 16th, 10:00 AMApr 16th, 12:00 PM

Examining the Interactions of Decanal and Trans-2-Decenal with Various Nucleotide Bases

CSU Ballroom

Chemical reactions from biological and synthetic factors often form adducts with DNA. According to K.A. Naidu, decanal and trans-2-decenal have been touted to potentially possess anti-cancer properties. The purpose of this research was to explore whether either of these aldehydes lead to the formation of adducts with DNA. These aldehydes were dissolved in deuterated dimethyl sulfoxide (d6-DMSO) with and without the presence of arginine and treated with the nucleotide bases 2’- deoxyadenosine and 2-deoxyguanosine. After heating, precipitate was removed by filtration and reaction mixtures were analyzed using 1HNMR and HPLC. Decanal and trans-2-decenal were also allowed to treat calf thymus DNA. This reaction was heated at 50°C. Following acid hydrolysis, the reaction mixture was also analyzed by HPLC. Identifying and further characterizing adducts that form will ultimately allow further research into the chemo preventive or carcinogenic properties of these aldehydes.

Recommended Citation

Ranals, Neil. "Examining the Interactions of Decanal and Trans-2-Decenal with Various Nucleotide Bases." Undergraduate Research Symposium, Mankato, MN, April 16, 2013. https://cornerstone.lib.mnsu.edu/urs/2013/poster-session-A/24