Introduction Due to the changing nature of dengue epidemiology and control, this study was conducted to determine and describe evidence of transovarial transmission of the dengue virus.

Methods This was a quantitative experimental study on the transovarial transmission of the dengue virus from field-collected Aedes aegypti in an animal model. Viremia was detected by reverse transcriptase-polymerase chain reaction. Mosquito homogenate was used for intracranial inoculation of the virus into suckling mice. The brains of the suckling mice were extracted and inoculated intraperitoneally into 3 to 4 week old mice for recording of disease manifestation.

Conclusion These observations indicate that transovarial transmission of dengue virus can occur in a highly urbanized locale like Quezon City where dengue cases are high, and virulence may translate into dengue disease when inoculated in an animal model.