serum deprivation of cell lines - (Nov/21/2011 )

I am stimulating RAW 264.7 macrophages with LPS and then assaying for TNF-a and IL-6. I am new to these types of experiments, so is use of serum free media necessary for this experiment or before the experiment?

-~guru-

Serum might have some immune factors which could interfere with your assays. Probably do a negative control if you have to include serum, i.e. cells without treatment.