Cardiolipin Biosynthesis CL(10:0/12:0/12:0/24:0)

Saccharomyces cerevisiae

Metabolic Pathway

The biosynthesis of cardiolipin begins in the endoplasmic reticulum. Glycerone phosphate interacts with a NADPH resulting in the release of NADP and glycerol 3-phosphate. Glycerol 3-phoshate reacts with Glycerol-3-phosphate O-acyltransferase resulting in the release of 1-palmitoylglycerol 3-phosphate. The resulting compound reacts with an acyl-CoA through a oleoyl-CoA: lysophosphatidate acyltransferase resulting in the release of a Phosphatidic Acid. Phosphatidic Acid is transported to the mitochondrial outer membrane. Once in, it gets transported into the mitochondrial inner membrane. The phosphatidic acid reacts with cytidine triphosphate through a phosphatidate cytidyltransferase resulting in the release of a CDP-DG. The resulting compound reacts with a glycerol 3-phosphate through a CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase resulting in the release of cytidine monophosphate and PGP. PGP reacts with Phosphatidylglycerophosphatase GEP4, mitochondrial resulting in the release of PG. PG reacts with a CDP-DG through a Cardiolipin synthase (CMP-forming) resulting in the release of CL and cytidine monophosphate. CL reacts with lipase cldp1 resulting in the release of 1-MLCL. This in gets transformed into the isomer 2-MLCL. This in turn can either react with an transacylase TAZ1P resulting in the release of CL or it can be transported to the mitochondrial outer membrane where it reacts with a transacylase TAZ1P resulting in the release of a cardiolipin.

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