Land application of poultry wastes in Kentucky will increase as the broiler industry grows. If poultry manure stimulates N2O loss from soil it will reduce the fertilizer N value of this waste. In contrast, stimulated N2O loss in grass filter strips receiving the runoff from manured fields could help reduce contamination of surface water by NO−3. Our objectives were to determine (i) if poultry manure stimulated N2O loss in soil after rainfall and (ii) if there was an edge-of-field effect on N2O loss in grass filters intercepting runoff from amended soil. Soil covers were used to measure N2O loss from a well-drained, poultry manure-amended, silt loam soil immediately after simulated rainfall and were also used to measure N2O loss from grass filters intercepting their surface runoff. Nitrous oxide loss from manure-amended soil was greater than from unamended controls and ranged from 5 to 13 mg N2O-N m−2 h−1. The maximum N2O loss was equivalent to 3.2 kg N2O-N ha−1d−1. Nitrous oxide loss from grass filters intercepting runoff ranged from 0.1 to 1.4 mg N2O-N m−2 h−1 and was significantly greater than portions of the grass filters that did not intercept runoff. Nitrous oxide loss from poultry manure-amended soils was greater than N2O loss typically measured from waste-amended agricultural soils. However, it only represented up to 0.7% of the total N in the applied manure.