7. Plate various amounts of cells on YPDS plates containing zeocin. There is not much point in plating very large volumes of cells (i.e. don’t plate more than 200 μl). They will make a lawn and there will be few discrete colonies.

8. Incubate plates at 30C for 2-6 days. Put a beaker of water in the incubator to keep plates from drying out.