Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age

adult

Gender

Male

Strain

C3H/HeJ

Storage Conditions

liquid nitrogen vapor phase

Derivation

The 2H-11 cell line was derived from a solid tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161).

The line was cloned in 1992 by limiting dilution.

Clinical Data

male

Antigen Expression

H-2 K; VCAM

Tumorigenic

Yes

Effects

Yes, the cells induce spindle tumors with some of the histopathologic characteristics of human Kaposi Sarcoma after a latency period of approximately 14 weeks

Comments

The cells are sensitive to lysis by activated macrophages as measured in the chromium release assay.

This clone retains the ability to differentiate on a synthetic basement-like membrane.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.