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The significance of erythrocyte antigen site density: II. Hemolysis

Abstract

The importance of antigen site density has been studied by means of a model passive hemolysis system using red cells coupled with sulfanilic acid groups. Relative site numbers were estimated from the covalent linkage of sulfanilic acid-35S to red cell membrane protein, and the effective antigen site number was determined with 125I-labeled rabbit IgG anti-sulfanilic acid (anti-S).