What's wrong with my Taylor?

Hey, so I have a Taylor guitar, and I'm having some trouble with it. I never used to have issues, but all of the sudden I took it out of its case (that does have a humidifier btw) and the 12 and 1st fret are in tune, but the 3 to the 5th-ish... are noticibly not. The neck looks straight. I have a truss rod wrench, should I tried tweaking it a little bit and seeing if that helps? I hopefully don't have to pay $60 for someone to set it up for me.... Thanks!

I would suspect the strings. Nothing should change the intonation of the guitar itself.... That's strictly a function of string length and fret placement.... Which should be pretty much spot on with a Taylor.

So something else is likely going on. Replace the strings. Then, using an electronic tuner, make sure your intonation is correct by playing the fretted note at the 12th fret and the harmonic at the 12th fret. They should be the same.If not, I'd check with Taylor for your local authorized repair center.