I have included below a summary of responses to my request
for information about automated sequencing costs. Many
thanks to all who responded!
--Jim
--
Jim Lissemore
Assistant Professor
Biology Dept.
John Carroll University
20700 N. Park Blvd.
University Heights, OH 44118
EMAIL: jlissemore at jcu.edu
PHONE: 216-397-4196
FAX: 216-397-4482
________________________________________________________________________________
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From:
Ahna Renee Skop <arskop at students.wisc.edu>
UW-Madison.
At the UW Biotech Facility it cost about $6 a run,
$2 for a column,
and $3.50 for seq rxn.
If you run the rxn yourself it is $6 a run...Look on the web
page below
Get rid of unincorporated fluorescent nts using a G50
Autoseq (Pharmacia)
Precip the DNA, you get a pellet and you hand them the
pellet and they post
the sequence on the web.
the web site is http://www.biotech.wisc.edu
You should email them about outside accounts. I think that
they do them
charles_nicolet at gene.biotech.wisc.edu
This guy heads up the sequencing stuff....
From:
Jenny Watts <jwatts at mail.wsu.edu>
We pay $12 per reaction at our facility at Washington State
Univ.
I called our sequencing guy. He says he will sequence for
people outside
the university, however he gives priority to people from
here, so it might
take a little longer.
From:
Anne Hart <hart at helix.mgh.harvard.edu>
Jim- At MGH we pay 20$ per reaction at the MGH core
sequencing facility for
400-600nt (supply your own primers.) The price may be
artificially low due
to funding from grants supporting core facilties as
researchers outside MGH
have to pay 35$ per reaction.
From:
Barth Grant <grant at cuccfa.ccc.columbia.edu>
Columbia University College of Physicians and Surgeons
Columbia charges $20 per run for DNA sequencing.
From:
"David H. A. Fitch" <df3 at acf2.nyu.edu>
I run our departmental core facility, and I'd say $30 is way
overpriced.
We prefer it if people do their own sequencing reactions and
we just load
the samples on our ABI 377--that's saves labor and time, and
core
facilities should allow this option. However, we do run a
full service
option, and the price can still be as low as $16 per
template. We only
sequence for our department, though, not for outsiders. NYU
medical school
has a more commercial facility, but they charge $25 per
template. I think
the price schedule mainly depends on what the personnel
cost.
In case you're curious, we just put up a site for our
facility:
http://www.nyu.edu/projects/fitch/wwwboard/
From:
Susan Mango <susan_mango at qmserver.hci.utah.edu>
$10 a run (i.e. pcr reaction and gel).
From:
patterson at mbcl.rutgers.edu (Garth Patterson)
At the facility here at Rutgers/UMDNJ, we pay $20 per
sequencing run. As
a nice bonus, we pay nothing if the sequencing run fails for
any reason.
From:
Leon Avery <leon at eatworms.swmed.edu>
We pay $25 / run, and routinely get about 700 readable
bases/run.
From:
Michael Herman <mherman at ksu.edu> Kansas State
University
We have been using the Ohio State Biopolymer Facility for
our DNA
sequencing. They charge $18.75 per reaction and we
generally get at
least 500 bp of good sequence. The email the results along
with a PDF
file of the automated sequencer output showing the peaks
which can be
quite useful. In general we have been happy with the manner
in which
they deal with reactions that do not produce data etc. You
can find
more info at:
http://ccic.mps.ohio-state.edu/biopolymer/molecular_biology.htm
From:
"R. John Lye" <rjl6n at server1.mail.virginia.edu>
Our core facility here charges $24 per sequencing run (they
supply common primers in that price).
From:
dna at unsw.EDU.AU (Alan Wilton)
At current exchange rates you could get it done in Australia
for $US10 if
you have a large number to do.
Do a web search for AGRF at Queensland University in
Brisbane.
From:
David Greenstein
<David.Greenstein at mcmail.vanderbilt.edu>
I saw your question about sequencing fees. We pay $10 per
template for "gel
run
only" service at our core (Vanderbilt University Cancer
Center). We do the
reactions ourselves using the Perkin-Elmer Big-Dye kit. Our
cost is
between
$2.50-$7.50 for doing the reactions depending on how much
sequencing
reagent is
used or whether commercially available spin columns are used
to remove un-
incorporated dye. This estimate does not include
oligonucleotide primer
synthesis. Hope this helps!
From:
Glenis Wiebe <gwiebe at ucalgary.ca>
Our company offers DNA sequencing for $35 (Canadian) per
reaction.
Samples are run on an ABI 377XL (48cm read), from which we
typically get
600-800 bases of good sequence (depending on the quality of
the
template). Results are posted to an FTP site, usually
within 2-3 days
from the time the sample arrives. For more information on
our services,
please see our web-site at
http://www.ucalgary.ca/~dnalab