* Understand the mathematics underlying the conversion from 16-bit to 8-bit.

### Activity: 16-bit to float conversion

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@@ -191,12 +190,12 @@ What are good reasons to change the pixel data type of an image?

### Formative Assessment

True or false?

True or false (discuss with your neighbor)?

1. Changing pixel data type never changes pixel values.

2. Converting from 16-bit unsigned integer to float never changes the pixel values.

3. Changing from float to 16-bit unsigned integer never changes the pixel values.

4. There is only one correct way to convert from 16-bit to 8-bit.

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@@ -204,7 +203,6 @@ True or false?

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## Thresholding

In order to find objects in a image, the first step often is to determine whether a pixel is part of an object (foreground) or of the image background. In fluorescence microscopy this often can be achieved by thresholding.