Production and Quality Assurance

After expression, the N-terminal GST- and His6-tagged proteins are purified and printed on glass slides in duplicate, along with control proteins (GST, BSA, Histones, IgG, etc.). Slides are barcoded for tracking/archiving. Each microarray batch is routinely evaluated by GST staining to ensure printing quality and content. Flexibility is built-in, meaning the HuProt™ human proteome microarray is available on two types of glass surfaces: PATH™ and SuperEpoxy 2™.

Focused arrays can be designed to feature biomarkers identified within the HuProt™ discovery phase. Efficient (up to 2x7) slide formats can be configured for a cost-effective and rapid validation phase.

Arrayjet's high throughput bio-printing platform, Ultra Marathon II, is used by CDI Labs to manufacture HuProt™ with significant performance advantages over contact print methods such as: