Application Information: ADAM10 antibody

Western blotting (1-4 µg/ml) and immunofluorescence (2-10 µg/ml). An 85 kDa band corresponding to the precursor form of ADAM10 can be detected. Jurkat and K562 cell lysates can be used as positive controls. However, the optimal conditions should be determined individually.

Form Supplied

Liquid

Concentration

0.5
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Affinity purified

Specifications: ADAM10 antibody

Product Description

Rabbit polyclonal antibody to ADAM10

Background

Proinflammatory cytokine tumor necrosis factoralpha (TNF-alpha ) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-alpha , notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-alpha , notch and its ligand delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM10, kuzbanian (KUZ), and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues.

Synonyms

CDw156, CD156c, EC3.4.24.81, HsT18717, kuz, MADM, EC 3.4.24.81

Specificity

This antibody is specific for ADAM10.

Host

Rabbit

Clonality

Polyclonal

Target

ADAM10

Isotype

IgG

Species Reactivity

Human

Storage Conditions: ADAM10 antibody

Storage Buffer

PBS (pH 7.2) containing 30% glycerol, 1% BSA, 0.02% thimerosal

Storage Instruction

The antibody solution should be gently mixed before use. Stored at -20 °C for future use.

Notes

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.