Application Details

Application Details

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Application Notes

ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 48 kDa band observed in lysates of Human Brain, Kidney and Testis (calculated MW of 42.8 kDa according to NP_006087.2). Recommended concentration: 0.1-0.3 µg/mL. A minor band of unknown identity was also consistently observed at 20 kDa. This band was successfully blocked by incubation with the immunising peptide. IHC: In paraffin embedded Human hepatocelluar carcinoma shows strong labeling of membranes and weaker staining of cyrtoplasm. These observations are consistent with those obtained by different antibodies. Recommended concentration: 0.5-2 µg/mL.