My confusion is 'should all the sequence (till the very start) before the 1st ATG in the 1st exon be considered 5' UTR or just this part:
CCCAGATAAAAGGTAGGGGAGGAGGAGAGAGAGAGAAGGAAGAGTCTAGGCTGAGCAAC after which there is an inframe ATG.

thanks

zhongmindai on Oct 17 2009, 11:14 PM said:

kedar on Oct 17 2009, 09:58 AM said:

shit, already 14 views and no answer!!

I dislike this kind of question.
Its your homework, not ours.
The url you give us is not the gene's mRNA. 5'UTR (5' untranslated region) and 3'UTR is relative to mRNA, not genomic DNA sequences.

-kedar-

kedar on Oct 18 2009, 03:11 AM said:

I dislike this kind of answer from a rude chinese. If somebody doesn't wanna help, they don't need to reply. Here we help each other, ok.

My confusion is 'should all the sequence (till the very start) before the 1st ATG in the 1st exon be considered 5' UTR or just this part:
CCCAGATAAAAGGTAGGGGAGGAGGAGAGAGAGAGAAGGAAGAGTCTAGGCTGAGCAAC after which there is an inframe ATG.

thanks

zhongmindai on Oct 17 2009, 11:14 PM said:

kedar on Oct 17 2009, 09:58 AM said:

shit, already 14 views and no answer!!

I dislike this kind of question.
Its your homework, not ours.
The url you give us is not the gene's mRNA. 5'UTR (5' untranslated region) and 3'UTR is relative to mRNA, not genomic DNA sequences.

"shit","rude", who used these words?
An mRNA contains 5'UTR, ORF and 3'UTR.CCCAGATAAAAGGTAGGGGAGGAGGAGAGAGAGAGAAGGAAGAGTCTAGGCTGAGCAACATGAAGGGGCCCCCAACCTTCTGCAGCCTCCTGCTGCTGTCATTGCTCCTGAGCCCAGACCCTACAGCAGCATTCCTACT
GCCACCCAGCACTGCCTGCTGTACTCAGCTCTACCGAAAGCCACTCTCAGACAAGCTACTGAGGAAGGTCATCCAGGTGGA
ACTGCAGGAGGCTGACGGGGACTGTCACCTCCAGGCTTTCGTGCTTCACCTGGCTCAACGCAGCATCTGCATCCACCCCCA
GAACCCCAGCCTGTCACAGTGGTTTGAGCACCAAGAGAGAAAGCTCCATGGGACTCTGCCCAAGCTGAATTTTGGGATGCT
AAGGAAAATGGGCTGAAGCCCCCAATAGCCAAATAATAAAGCAGCATTGGATAATAATTTCTGAAAAAAAAAAAAAAAAAAAAAAAA

-zhongmindai-

thanks , that's the spirit. I take back the rude word.

My answers are the same but once somebody else tells something else, it becomes a mess, you know. So, i needed to confirm.

thanks

-kedar-

next question!

if you are asked to count the number of nt in 3' UTR, will you count AGCCCCCAATAGCCAAATAATAAAGCAGCATTGGATAATAATTTCTGA? =48 nucleotides
or AGCCCCCAATAGCCAAATAATA
AAGCAGCATTGGATAATAATTTCTGAAAAAAAAAAAAAAAAAAAAAAAA? because the aaaa... i guess is poly a tail. and i guess you can add more AAAs...