Using acetylated and methylated peptides synthesised at Cambridge Research Biochemicals it has been demonstrated that there is an interplay between NF-κB subunit Re1A acetylation and methylation.

Post-translational modifications of the Re1A subunit of NF-κB play a key role in controlling its nuclear activity. These modifications include acetylation and methylation. In this work chemically synthesised peptides with and without acetylation and methylation modifications have been used to establish that acetylation of lysine 310 of Re1A impairs the Set9-mediated methylation of lysines 314 and 315, which is important for the ubiquitination and degradation of chromatin–associated Re1A. NF-κB plays an important role in regulating inflammatory responses, apoptosis, cell proliferation and differentiation and tumorigenesis.

Standard peptides are made up of naturally occurring L-amino acids (of which there are twenty) and have a length of 6-25 residues. We offer two purity standards: >80% and >95% by HPLC and can synthesise laboratory-scale amounts from 1mg – gram scale quantities. Turnaround is 2-3 weeks.

Peptide Applications

Within the field of biology chemically synthesised peptides are a very powerful research tool. Peptides can be used in wide-ranging applications both in-vitro and in-vivo to investigate biological pathways and hence are an integral part of the drug discovery process. Some typical applications our peptides are used for are:

Production of anti-protein antibodies where a peptide is used as a surrogate for the protein

Enzyme activity studies in which a peptide substrate is used to investigate actions of enzymes such as kinases which are critically important in cancer research

Binding studies in which a fluorescently labelled or radiolabelled peptide is used to study binding of the peptide ligand to proteins

Study of post-translational modifications such as phosphorylation and methylation where these modifications can be chemically included within a peptide

Quality

Purity: >95% by HPLC

Peptide identity confirmed by mass spectrometry (ESI or MALDI-TOF)

In-process control of the synthesis of complex peptides by mass spectrometry and HPLC monitoring

Customer Support

At CRB we pride ourselves on the quality of our peptides and our customer focussed service:

Once issued with a password you can then log on to our quotation system using your customer account log-in and a quotation will be issued within 24 hours.

At Cambridge Research Biochemicals we have a fast-track quotation system that tailors your requirements, gives you all the information you need and a specific price within 24 hours. You are also given a personal contact so that if you have any further questions you can directly contact one of our approachable representatives who has the technical knowledge to assist you.

If you would like any support, help or technical advice we would like to hear from you.