my questions are as following.
1. is the [H2O2] too high? Should I use a lower concnetration
2. is the phen red medium good for DCF-DA
3. What about HBSS or PBS for 24 h treatment?
4. Would you use DCFDA before the H202 or would you stress the cells before with H2O2 and then reading the fluorescence after 30 minutes of DCFDA?