Encoded by the PES1 gene, pescadillo is a nuclear protein that contains a breast cancer associated gene 1 (BRCA1) C-terminal interaction domain. The encoded protein interacts with BOP1 and WDR12 to form the PeBoW complex, which plays a critical role in cell proliferation via pre-rRNA processing and 60S ribosomal subunit maturation. Expression of PES1 may play an important role in breast cancer proliferation and tumorigenicity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for PES1. Pseudogenes of PES1 are located on the long arm of chromosome 4 and the short arm of chromosome 9 (provided by RefSeq, Aug 2011).

Goat anti Human pescadillo antibody detects a band of 68 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti pescadillo detects a band of approximately 68 kDa in MCF7 cell lysates.

Species Cross-Reactivity

Target Species

Cross Reactivity

Mouse

Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Goat polyclonal antibody purified by affinity chromatography.

Preparation

20μl Goat polyclonal antibody purified by affinity chromatography

Buffer Solution

TRIS buffered saline

Buffer Solution

TRIS buffered saline

Preservative Stabilisers

0.02% Sodium Azide (NaN3) 0.5 % BSA

Preservative Stabilisers

0.02% Sodium Azide (NaN3) 0.5 % BSA

Immunogen

Peptide with the sequence GGLEKKKYERGSA-C, from the N Terminus of the protein sequence.

Applications of Pescadillo antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name

Verified

Min Dilution

Max Dilution

Western Blotting

1/1000

Western Blotting

1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.