Seattle WA (SPX) Jun 20, 2005Researchers report today that regions of the human genome have been hotspots for acquiring duplicated DNA sequences ¿ but only at specific time-points during evolution.

It appears that long periods of genomic stasis, at least with regard to the accretion of duplicated DNA fragments, are "punctuated" by relatively brief episodes of duplicative activity.

This is the first time that such temporal bias has been documented for DNA duplications, and it challenges the evolutionary paradigm that continuous alterations occur during the course of genome evolution.

The scientists, who are affiliated with the University of Washington (Seattle, WA), Case Western Reserve University (Cleveland, OH), the University of Bari (Bari, Italy), Washington University (St. Louis, MO), Washington State University (Pullman, WA), and Duke University (Durham, NC), report their findings online today in the journal Genome Research.

Dr. Evan E. Eichler, Associate Professor of Genome Sciences at the University of Washington, heads the team. "Primate genomic sequence comparisons are becoming useful for elucidating the evolutionary history and organization of our own genome," he explains.

Pericentromeric regions are sequences of DNA that lie in close proximity to the centromere, which plays a critical role in chromosomal separation during cell division.

Pericentromeric regions contain an abundance of segmental duplications, which are large DNA sequences that exhibit strong similarity to the euchromatic ancestral loci from which they were copied.

According to Eichler, the limited number of comparisons of pericentromeric regions among closely related primates suggests extraordinary dynamism, where duplication, deletion, and rearrangement of large segments of DNA occur at an unprecedented scale.

Eichler's group performed a comprehensive structural and evolutionary analysis of a 700-kilobase (Kb) pericentromeric region on the short arm of human chromosome 2. This chromosome has intrigued evolutionary and primate biologists for years because it appears to have formed from the fusion of two mid-sized ape chromosomes, and it is the primary cytogenetic distinction separating humans and their evolutionary progenitors.

Within this 700 Kb region of human chromosome 2, the researchers identified segments of DNA that originated from 14 ancestral loci. These DNA segments, or "duplicons," ranged from 4-77 Kb in length and exhibited 94-99% sequence identity to their euchromatic predecessors.

The scientists then performed a comparative analysis of these duplicons in other primate species, including chimpanzee, gorilla, orangutan, baboon, and macaque.

This analysis revealed that the duplicative transposition events leading to the establishment of these duplicons within the pericentromeric region occurred during a relatively narrow window of evolutionary time between 10-20 million years ago.

This corresponds to the time period following the divergence of humans and Old World monkeys, but before the divergence of humans and great apes. For the past 10 million years, however, no such "duplicative seeding" events appear to have occurred in this region of the genome.

"It is unclear why pericentromeric seeding events have occurred so frequently during this period of human/great-ape evolutionary history," says Eichler. "It is also unclear as to why they suddenly cease, at least in the case of this pericentromeric region of chromosome 2."

Clearly, factors other than DNA sequence are necessary for such "punctuated" duplicative transposition events to occur during genome evolution. During the divergence of the human/great-ape lineage from the Old World monkey lineage, the genome may have been particularly permissive to segmental duplication events.

The scientists speculate that the molecular driving forces behind this "punctuated" duplicative activity may have been changes in transcriptional status or chromatin conformation.

"Other regions may show different temporal biases," explains Eichler.

"The important implication here is that episodic bursts of activity challenge the concept of gradual clock-like changes during the course of genome evolution. Since duplications are important in the birth of new genes and large-scale chromosomal rearrangements, it may follow that these processes may have gone through similar episodes of activity followed by quiescence."

More Than One Million Americans Infected With AIDSWashington (AFP) Jun 14, 2005More than a million Americans were infected with AIDS at the end of 2003, with black, homosexual and bi-sexual men making up the largest group among them, according to government statistics made public Monday.

The content herein, unless otherwise known to be public domain, are Copyright 1995-2006 - SpaceDaily.AFP and UPI Wire Stories are copyright Agence France-Presse and United Press International. ESA PortalReports are copyright European Space Agency. All NASA sourced material is public domain. Additionalcopyrights may apply in whole or part to other bona fide parties. Advertising does not imply endorsement,agreement or approval of any opinions, statements or information provided by SpaceDaily on any Web page published or hosted by SpaceDaily. Privacy Statement