Bioinformatics day-by-day

Menu

MinION fast5 to fastq

Finally, I got my first data from MinION. The first obstacle I found is the native MinION format, Fast5. Most programs require FastQ. Luckily there are poretools, which makes conversion Fast5 –> FastQ very easy.

Post navigation

4 thoughts on “MinION fast5 to fastq”

We recently finished a MinION sequencing experiment and got our first fast5 files. We are having difficulty converting them to fastq files. Would you be able to send some pointers on how we can do the conversion? I would greatly appreciate your help.