SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.

E14 mouse brain sample was stained with primary antibody (ab66155). The secondary antibody used was ab96919 (1:200 dilution, incubate for 1 hour at room temperature). The blue background was from DAPI.

I think this information would need to be obtained from the manufacturer of the microscope and filters that the customer is planning touse. They would be able to exactly tell the customer which wavelengths can be...

DISCOUNT CODE: 42087 Expiration date: 28/05/2012 I am very pleased to hear you would like to accept our offer and test ab96919 in IHC-Fr. This code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date. ...

Thank you for your enquiry and your interest in our products. I can certainly offer a discount code for testing this secondary antibody for IHC on frozen sections. Could you please pass your customer's contact details for our internal record ...

Thank you for providing that extra information. Out of the secondaries used, the DyLight488 (ab96919) definitely seems to be producing more diffuse, non-nuclear staining compared to the other three. It may be that this could be reduced by optimising th...

Thank you for taking time to complete our questionnaire. I am sorry to hear that the Donkey anti-Rabbit IgG H&L (DyLight® 488) secondary antibody (ab96919) is not providing satisfactory results. The details provided will enable us to investigate this c...