UV Spectrophotometric method for the estimation of nebivolol HCL in bulk and pharmaceutical formulations

Abstract

In this study, a simple, sensitive and highly accurate ultraviolet pectrophotometric
method has been developed and validated for determination of nebivolol
hydrochloride in bulk and pharmaceutical formulations. The method is based on
the measurement of the absorbance of nebivolol hydrochloride solution in
methanol: phosphate buffer pH 6.8 (10:90) at 282 nm in the wavelength range of
200 - 400 nm. Beer’s law was obeyed in the concentration range of 10-50 μg/mL.
The slope, intercept and correlation coefficient were also calculated. Results of
percentage recovery shows that the method was not affected by the presence of
common excipients in tablets. The developed method was validated in terms of
accuracy, precision, linearity, limit of detection, limit of quantification which
proves suitability of proposed method for routine estimation of nebivolol HCL in
bulk and pharmaceutical formulations.