The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.Tags: Manuals & User Guides

Q&A

PAH Extraction

09 March 2016

Question:

What concentration of PAH's do I get after strain by means of ISOLUTE 1,5 g PAH filter if i have passed through it 500 mL sample?

Answer:

This is an open ended question as it depends on the initial concentration of the PAH compounds in the water sample and the final volume of your finished extract both of which are unknown. Let me outline with a couple of examples.

Water sample contains various PAHs at 5 ng/L per component

We extract 500 mL through the ISOLUTE PAH column

We elute the analytes from the column using a suitable solvent and then concentrate the resulting eluent

If we concentrated down the eluent to 1 mL final volume we have achieved a x 500 concentration factor and analytes are now present in the final solution at 2.5 ng/mL.

Alternatively

If we concentrated down the eluent to 0.1 mL final volume we have achieved a x 5000 concentration factor an analytes are now present in the final solution at 25 ng/mL.

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Biotage is a global Life Science company that develops innovative and effective solutions for separation within organic and analytical chemistry, as well as for industrial applications. Biotage is listed on NASDAQ Stockholm.