The five typical aspens are all native to cold regions with cool summers, in the far north of the Northern Hemisphere, extending south only at high altitudes in mountains. The White Poplar by contrast is native to much warmer regions, with hot, dry summers. They are all medium-sized deciduoustrees reaching 15-25 m tall, exceptionally to 30 m.

Aspens (apart from the aberrant White Poplar) are distinguished by their nearly round leaves on mature trees, 9-16 cm diameter with irregular rounded teeth. They are carried on strongly flattened leaf stems, which enable the leaves to twist and flutter in the slightest of breezes. The juvenile leaves on young seedlings and root sprouts differ markedly from the adult leaves, nearly triangular, showing here the typical leaf shape of most other poplars; they are also often much larger, 13-26 cm long. The five typical aspens are distinguished from each other by leaf size and the size and spacing of the teeth on the adult leaves. White Poplar leaves differ in being deeply five-lobed, covered in thick white down, and having only a slightly flattened leaf stem.

All the aspens (including White Poplar) typically grow in large colonies derived from a single seedling, and spreading by means of root suckers; new stems in the colony may appear at up to 30-40 m from the parent tree. Each tree only lives for 40-150 years above ground, but the root system of the colony is long-lived, in some cases for many thousands of years, sending up new trunks as the older trunks die off above ground. For this reason it is considered to be an indicator of ancient woodlands. One such colony in Utah, given the nickname of "Pando", is claimed to be 80,000 years old, making it possibly the oldest living colony. Some aspen colonies become very large with time, spreading about a metre per year, eventually covering many hectares. They are able to survive intense forest fires as the roots are below the heat of the fire, with new sprouts growing after the fire is out. However, aspens do not thrive very well in the shade, and it is difficult for aspen seedlings to grow in an already mature aspen stand. Fire indirectly benefits aspen trees, as it allows the saplings to flourish in open sunlight on account of the burned landscape. Lately aspen has increased its popularity in forestry, mostly because of its fast growth rate and ability to regenerate from sprouts, which makes the regeneration of the forest after harvesting much cheaper, as no planting or sowing is required.

The unusual ability of the leaves of Populus to twist and bend due to the flattened petioles may not be fully understood. It is thought to help protect the trees from severe winds, perhaps by helping dissipate energy more uniformly throughout the canopy. It is also thought to improve the rate of photosynthesis throughout the tree by reducing the exposure of the outer leaves to extreme sunlight (thus reducing photoinhibition) by presenting the leaves at an oblique angle to the sun throughout the day, while at the same time allowing more light through to the lower leaves which are generally overshaded. This would enable leaves throughout the tree to photosynthesize more efficiently.

The aspen tree's quivering leaves are, in Christian lore, said to be the result of arrogance at the Crucifixion because the aspen did not tremble like other trees. A German version claims that the aspen was the only tree to refuse to acknowledge the divinity of Jesus. The cross that Christ was crucified on is sometimes said to have been aspen wood. As aspens do not occur in Palestine, this legend is improbable. Another old saying was that aspen leaves are made from female tongues, and their quivering is due to women's inability to stop talking.

The wood is white, and soft, but fairly strong, and with very low flammability. It has a number of uses, notably for making matches, where its low flammability makes it safer to use (easy to blow out) than most other woods. Shredded aspen wood is also a popular animal bedding, as it lacks the phenols associated with pine and juniper, which are thought to cause respiratory ailments in some animals. Heat treated aspen is a popular material for the interiors of a sauna.