1All the authors are with the University of Texas Medical Branch, Galveston, Texas 77555-0456. R. Kuranov, V. Sapozhnikova, I. Cicenaite, and R. Esenaliev
(riesenal@utmb.edu) are with the Center for Biomedical Engineering, Laboratory for Optical Sensing and Monitoring. D. Prough, as well as R. Esenaliev, is with the Department of Anesthesiology. R. Esenaliev is also with the Department of Neuroscience and Cell Biology.

Abstract

Noninvasive imaging techniques such as optical coherence tomography (OCT) are being widely used for early diagnostics of a variety of pathologies. Traditional tissue preparation for histological evaluation alters the dimensions of histological images such that differences between the dimensions of histologic and OCT images can be as great as 2.5-fold. This discrepancy complicates quantitative interpretation of OCT images. Here we used a steel ring sewn to the pigskin to avoid tissue deformation due to excision and fixation and used a surgical suture as a fixed tissue marker. With these techniques, we achieved good correlation between OCT and histological images with differences of less than 10%. The resulting method significantly enhances the diagnostic capabilities of the OCT technique.

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