Voltage gated potassium channels play crucial roles in excitable cells. KCNQ channels belong to the potassium channels and are expressed in the central nervous systems. However, little is known about the physiological roles. Electrophysiological recordings were done from cerebellar acute brain slice preparations. Application of a KCNQ channel blocker or opener reveals that KCNQ channels play roles in maintaining the membrane potential and neuronal excitabilities of cerebellar neurons, suggesting KCNQ channels contribute to the function of the motor coordination conveyed by the cerebellum.