The objective of this research was to determine the effectiveness of the multiplication of micropropagated strawberry stock plants as a method for producing runner tips to be used in the production of plug transplants. Micropropagated stock plants of cultivars INIA-Arazá, INIA-Guenoa and INIA-Yvapitá were planted at October 1st, 2008. Runner tips emitted by the micropropagated stock plants were harvested and planted as multiplied stock plants at 37; 40; 46; 51; 58; 64; 67; 75 and 87 days after planting the micropropagated stock ones. Runner tips emitted by all plants between October, 2008 and April, 2009, were harvested and counted, the number of root nodules counted and the crown diameter measured. The number of runner tips decreased linearly on all stock plants by delaying planting time, while crown diameter and number of root nodules were not affected. Higher number of runner tips was produced by micropropagated stock plants. The cultivar INIA-Arazá was the most prolific. Multiplying stock plants may be a new propagation method than can be used for reducing the number of micropropagated stock plants required for the production of strawberry commercial plug transplants.