Treq-Blue and purity

Treq-Blue is the purest of all dyes tested!

Treq-Blue is produced from highly purified TryphanBlue with a concentration of
0,06% (0,6 mg/ml). Vitreq has developed a new, unsurpassed purification process to effectively remove the colored and uncolored impurities in an elaborate two-step purification process. Ultra-purified dye increases the safety and staining effect.

Test specifications
Purity of the dyes was monitored by HPLC chromatography, carried out at Department of Life Sciences and Chemistry, Jacobs University Bremen, Bremen, Germany.

A C18 Gravity column from Macherey-Nagel was used. The eluent was a linear gradient from 0.02 M phosphate buffer pH = 6.0 to 100% acetonitril. Flow rate was 1.0 mL/min, and detection at 530 nm (absorption for pink dye impurities). Chromatography traces were integrated with the Clarity Lite software, using identical thresholds and a valley-to-valley baseline. The areas of the integrated peaks were summed up, and the sums of the percentages of the minor peaks are listed in the above table.