Kinetics

Goals

Procedure

We ran multiple UV-Vis spectras of the HRP with the gold nanoparticles to examine the impact that the NPs had on the kinetics of the reaction.

Data

We ran 5 main tests and several of the tests multiple times to confirm the data. The multiple tests showed that the experiment was not really reproducible because we seemed to be getting different spectras every time.

Trial run A

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Cuvet

hydrogen peroxide concentration (mM)

Iodophenol in DMSO concentration (μM)

AAP Concentration (μM)

HRP concentration (μM)

μL of gold np added

μL of water added

1( standard)

1.7

18

156.25

2.3

0

630

1

1.7

18

156.25

2.3

20

610

2

1.7

18

156.25

2.3

40

590

3

1.7

18

156.25

2.3

60

570

4

1.7

18

156.25

2.3

80

550

5

1.7

18

156.25

2.3

100

530

Total volume added

750 μL

10 μL

35 μL

22 μL

The amount of HRP was changed from 22μL to 33μL because the 22μL in the cuvet did not give a linear spectra, just a horizontal line. More was needed in order to really push the reaction.