The diode array detector signal was monitored at 240 and 280 nm, with a reference wavelength of 360 nm. The amount of norfloxacin in cultures was assessed by comparing the integrated area of the norfloxacin peak at 280 nm with that for the sterile controls. The amounts of metabolites were estimated by comparing the integrated area of each metabolite peak in the hplc chromatogram at 240 or 280 nm to the integrated area of the norfloxacin peak for the sterile controls at the same wavelength. All amounts are expressed as percentages of the peak source area of norfloxacin for the sterile controls. Effects of reducing agents and free radical scavengers. Norfloxacin (30 mg liter 1 ) was added to cultures in om medium at time zero. Gsh, dtt, and nadh (1.0 mm) were added after 7 days. In another experiment, ascorbic acid (0.05, 0.2, 0.5, or 1.0 mm) was added after 6 days. After incubation for 14 and 21 days, cultures and controls were analyzed by hplc. The free radical scavengers mannitol, sodium formate, and thiourea (24.
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