ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

Function

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

Tissue specificity

Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.

A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.Phosphorylated.

Cellular localization

Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.

Images

Flow Cytometry - Anti-CD163 antibody [GHI/61] (ab111250)

Staining of normal Human peripheral blood cells with 0.25 ug of Purified Mouse IgG1, K isotype control (open histogram) or 0.25 ug of ab111250 (colored histogram) followed by FITC Anti-Mouse IgG. Cells in the monocyte gate were used for analysis.

IF by definition refers to any antibody application that utilizes fluorescence as the detection method. Thus, technically ICC and IF are one in the same, and you could argue that IHC is also a form of IF.

For the purposes of searching for products on our website, we distinguish those antibodies that have been tested in IHC with IHC followed by a letter designation (P-paraffin embedded sections, Fr-frozen sections, FoFr-formaldehyde fixed frozen sections). If an antibody has ICC or ICC/IF listed under tested applications they are the same thing and guaranteed to work in immunocytochemistry.

It sounds like you are performing IHC-P, immunohistochemistry on paraffin embedded sections; thus, all three antibodies that you identified, ab53003, ab8216, and ab111250 are tested and guaranteed to detect their respective targets via IHC-P in human samples. As stated in our AbPromise guarantee, we are happy to provide scientific support, replacement or refund should these products if they do not perform as indicated on the datasheet. More information on our Abpromise may be found at the following link: