I am making constructs with pBI121 to transform Arabidopsis. I have the
whole sequence of the T-DNA region (-5.6Kb) of this plasmid. However, I
also need the restriction enzyme-cut map of the rest. Does anyone have
the sequence of the other part or any information on enzyme digestion of
this plasmid? Especially on what enzymes do not cut this plasmid? Please
send me a message by e-mail. Your help is earnestly needed and greatly
appreciated.
Hui-Rong Qian
Iowa State University
e-mail: qianhr at iastate.edu