The Stimulatory Effect of the Antibiotic Cefotaxime on Plant Regeneration in Maize Tissue Culture

The Stimulatory Effect of the Antibiotic Cefotaxime on Plant Regeneration in Maize Tissue Culture
Danilova, S.; Dolgikh, Yu.
2004-09-26 00:00:00
In order to elucidate the effects of the antibiotic cefotaxime on callus growth and morphogenesis, we incubated embryogenic maize calli (Zea mays L.) of A188 and R91 lines and of their F1 hybrid with 50–500 mg/l cefotaxime throughout several subcultures. Cefotaxime did not affect the induction frequency and growth of the embryogenic callus but enhanced its morphogenesis. In both tested lines and a hybrid, the highest increase in the number of regenerated plants was observed at the antibiotic concentration of 150 mg/l. The degree of morphogenesis stimulation and the range of cefotaxime concentrations effective in stimulation of plant regeneration depended on the properties of calli obtained from tested genotypes.
http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.pngRussian Journal of Plant PhysiologySpringer Journalshttp://www.deepdyve.com/lp/springer-journals/the-stimulatory-effect-of-the-antibiotic-cefotaxime-on-plant-10WKOEPflf

The Stimulatory Effect of the Antibiotic Cefotaxime on Plant Regeneration in Maize Tissue Culture

Abstract

In order to elucidate the effects of the antibiotic cefotaxime on callus growth and morphogenesis, we incubated embryogenic maize calli (Zea mays L.) of A188 and R91 lines and of their F1 hybrid with 50–500 mg/l cefotaxime throughout several subcultures. Cefotaxime did not affect the induction frequency and growth of the embryogenic callus but enhanced its morphogenesis. In both tested lines and a hybrid, the highest increase in the number of regenerated plants was observed at the antibiotic concentration of 150 mg/l. The degree of morphogenesis stimulation and the range of cefotaxime concentrations effective in stimulation of plant regeneration depended on the properties of calli obtained from tested genotypes.

Journal

Russian Journal of Plant Physiology
– Springer Journals

Published: Sep 26, 2004

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References

The Production of Callus Capable of Plant Regeneration from Immature Embryos of Numerous Zea maysGenotypes

Duncan, D.R.; Williams, M.T.; Zehr, B.T.; Widholm, J.M.

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