In a direct ELISA against purified Goat IgG 10 ug/ml as sample, conjugate diluted 1/25,000 produced an O.D.=1.0 in a 15 minute reaction with TMB as substrate. Optimal conjugate working dilutions should be determined for your particular assay conditions. Rabbits were immunized with purified Goat IgG in Freund's adjuvant. Anti IgG was immunoaffinity purified and conjugated with horseradish peroxidase (HRP).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Target

Goat IgG (H+L)

Immunogen

Purified Goat IgG

Antigen Species

Goat

Species Reactivity

Goat

Applications

ELISA, IHC, WB

Application Note

Optimal conjugate working dilutions should be determined for your particular assay conditions.

Conjugation

HRP

Form Supplied

Liquid

Concentration

1
mg/ml
(Please refer to the vial label for the specific concentration)

Storage Buffer

PBS, pH 7.2 with 0.2% BSA, 0.1% sodium azide

Storage Instruction

Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.

Notes

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.