Robust – reliable amplification in the presence of inhibitors and with even the most challenging DNA targets

Flexible – ideal for amplifying any target up to 5 kb, including DNA extracted from human, animal and plant samples

Fast – developed to give sensitive, reproducible and robust amplification of a broader range of targets under fast thermal cycling conditions

Convenient – includes all the components necessary for high performance PCR amplification

Product Description

MyTaq™ DNA Polymerase is recommended for all standard PCR applications. The MyTaq DNA Polymerase and MyTaq Reaction Buffer in this product, are a unique combination of next-generation polymerase and novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq has an increased affinity for DNA, enabling reliable amplification from very low amounts of template. MyTaq DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases allowing it to perform well with challenging templates in the presence of PCR inhibitors.

The composition of the buffer system is critical for efficient PCR. MyTaq reaction buffer contains dNTPs, MgCl2 and enhancers at optimal concentrations, which helps eliminate the need for optimization, saving time, effort and the cost of performing unnecessary assay repeats.

The combination of MyTaq and optimized buffer system allow for faster PCR reactions compared with other polymerases, therefore reducing overall run time from approximately 1 hour to under 30 minutes. This is achieved without compromising specificity or yield, reducing the reaction time allows for increased throughput and faster time to results.

A 450 bp fragment of the human myc gene (61% GC rich) was amplified under fast conditions using MyTaq with a DNA polymerase from supplier S. The PCR was performed using both enzymes and a three-fold decreasing amount of human genomic DNA as template (200 ng - 30 pg; lanes 1-8 respectively). Marker is HyperLadder 1kb (M). MyTaq amplifies the target more efficiently under fast conditions, resulting in higher yield, without the need for further optimization.

Highly efficient and robust amplification under a broad range of PCR conditions.

We use MyTaq DNA Polymerase for our mouse colony PCR screening. My established PCR protocols almost always work, and the run is shorter. Even new PCR protocols are much easier to establish since this polymerase is more robust than many others. We found that in several instances, if nothing works, this polymerase does.

Monica Kiela, University of Arizona, Tucson, US

Product Selection

Please refer to the PCR Selection Chart to confirm the recommended product for your PCR application.

FAQs

Yes, MyTaq DNA Polymerase does not possess 3' to 5' exonuclease activity, and therefore leaves an A-overhang at the 3' end, thus making the PCR product suitable for integration into TA cloning vectors.

The MyTaq HS DNA polymerase works very well with difficult samples like GC rich or bisulfite converted DNA. Especially the hot-start is important, as it decreases primer dimer and unspecific amplification. We would suggest to start with the recommended protocol. If optimization is needed, we would suggest to optimize the annealing temperature. Due to the degraded DNA after bisulfite conversion it may be useful to increase the amount of template and polymerase.

If possible, we would recommend an additional cleanup of affected samples, for instance, customers had very good experiences with the clean-up of samples containing humic acids with SureClean Plus. Alternatively decreasing the template concentration will help to minimize the amount of inhibitors, the amount of polymerase and primer can be increased, but do not exceed the suggested limits.

PCR can be a challenging technique, with various parameters to optimize to achieve the best results. If you are having problems, these could be easily resolved by addressing a few issues. Please see our PCR troubleshooting guide for suggestions and help with your specific problems.

These terms refer to parameters to be considered when performing PCR and are important features in selecting the correct enzyme for your needs. Understanding what they mean is therefore crucial:
Yield: The amount of DNA produced in a PCR reaction.
Fidelity: The accuracy of the enzyme at incorporating the correct dNTP to the elongating DNA strand.
Processivity: The length of time a polymerase is associated with the template and therefore the size of fragment which can be amplified.
Specificity: A measure of the unwanted by-products generated in a reaction.

During PCR setup at room temperature, standard polymerases will have some activity. When using a hot-start polymerase, this enzyme will have no activity at room temperature, thus reducing the risk of unspecific products in the reaction due to these mis-primed oligonucleotides. This feature makes this type of polymerase ideally suited to high-throughput applications, where the reactions may be left sitting at room temperature for prolonged periods of time.

All Bioline polymerases are available in the convenient format of a 2x master mix, containing all the reagents and additives necessary to perform successful PCR, with the exception of template, primers and water.
This formulation not only provides a convenient and hassle-free PCR setup, but also significantly reduces the chances of human error, inaccuracies and contamination by reducing the pipetting steps required.

The MyTaq HS DNA polymerase works very well with difficult samples like GC rich or bisulfite converted DNA. Especially the hot-start is important, as it decreases primer dimer and unspecific amplification. We would suggest to start with the recommended protocol. If optimization is needed, we would suggest to optimize the annealing temperature. Due to the degraded DNA after bisulfite conversion it may be useful to increase the amount of template and polymerase.

""With MyTaq polymerase we were able to clone and verify sequence to a ... "

"Studying pollen-mediated gene flow means I have very limited sample volumes, MyTaq is the only polymerase I have found that can cope with this efficiently."

Stirling University, Scotland, UK

"With MyTaq polymerase we were able to clone and verify sequence to a herpes virus that we otherwise would have had to give up on using any other polymerase."

San Diego Zoo, USA

"MyTaq proved to have better sensitivity and reproducibility: reliable and easy to use."

Cornell University, USA

"There is a little less smearing with MyTaq and there is a higher yield."

Brigham Young University, USA

"MyTaq DNA Polymerase “We like it.""

Uni Lübeck, Germany

"MyTaq produced a brighter band than the iTaq. Specificity appeared the same."

American Type Culture Collection (ATCC), USA

"Much better amplification was observed with MyTaq (than GoTaq) when all other conditions were equivalent."

Arbovax, Inc., USA

"MyTaq performed better than our current Taq polymerase on various mammalian nuclear and mitochondrial DNA targets, non-specific priming was reduced while sensitivity and yield were improved."

Robert Gordon University, Aberdeen, Scotland, UK

We use MyTaq DNA Polymerase for our mouse colony PCR screening. My established PCR protocols almost always work, and the run is shorter. Even new PCR protocols are much easier to establish since this polymerase is more "robust" then many others. We found that in several instances, if nothing works, this polymerase does.