To support cross-cultural attitudinal comparisons, one essential issue is to examine whether an instrument “works” in different contexts, since students from different backgrounds may interpret items differently. A short version of the Attitude toward the Subject of Chemistry Inventory (ASCIv2) was administered as a paper-and-pencil survey to college chemistry students in the U.S., Australia and Saudi Arabia. ASCIv2 captures responses on seven-point semantic differential scale to eight items regarding intellectual accessibility and emotional satisfaction, reflecting the cognitive and affective components of a tripartite attitude framework. Psychometric evidence, including reliability and validity based on internal structure, indicates that, while the instrument performs similarly in Australia and the U.S., problems with one item emerged in Saudi Arabia. Subscale results for the three countries will be compared and effect sizes will be interpreted. This study will be of interest to those who want to collaborate to build knowledge internationally about tertiary STEM education.