This list of characters from the Assassin's Creed franchise contains only characters that are considered part of Assassin's Creed canon. Some of them are completely fictional and some are partially based on real-world historical figures.

Desmond Miles (March 13, 1987[1]–December 21, 2012),[2] a member of the Assassin Order in the 21st century and a descendant of numerous familial lines that had sworn an allegiance to the Assassins; including individuals such as Aquilus, Altaïr Ibn-La'Ahad, Ezio Auditore de Firenze, Edward Kenway and Connor/Ratonhnhaké:ton. He ended his own life for the sake of the planet in 2012. He is voiced by actor Nolan North.[3] He chose death to protect humankind from a solar flare or billions would die. He released Juno in the process (Assassin's Creed III).

Altaïr Ibn-La'Ahad (1165–1257) was a Syrian-born member of the Levantine Brotherhood of Assassins who served as their Mentor from 1191 until his death in 1257. During his tenure as Mentor and its brief interregnum, Altaïr made several discoveries and inventions that greatly helped the Order's progression. Under his leadership the Levantine Assassins' spread their influence across the Old World, setting up many Guilds in cities like Constantinople. With the Apple in hand, Altaïr changed the way members of his Order lived their lives, writing the details in his fabled Codex for later generations of the Order to read. Throughout his travels, Altaïr strengthened his Order, stopping various Templar plots over the years as well as halting the inexorable march of Genghis Khan. Altaïr is an ancestor to Desmond Miles, through the maternal line. He is voiced by Philip Shahbaz in Assassin's Creed[3] and by Cas Anvar in Assassin's Creed Revelations.

Dr. Warren Vidic (voiced by Philip Proctor)[3] (unknown–2012) was the head of the Abstergo's Animus project and a member of the Templar Order. As the head of research for Abstergo, Vidic was put in charge of the genetic memory research and the Animus project. As the one responsible for finding new subjects for the Animus, Warren would have them explore their genetic memories, which he would then analyse to gain information on both the Assassins and the Pieces of Eden. As a high-ranking employee of Abstergo Industries, Warren was one of the few members of the Inner Sanctum, a group of Templars with full awareness of the Orders' plans for their New World. He played an important role in those plans, being tasked with finding a Piece of Eden to power Eye-Abstergo, a satellite which uses the Piece of Eden's power to control humanity. He was killed by Desmond Miles in Assassin's Creed III. In Assassin's Creed IV: Black Flag, an e-mail sent by Abstergo Entertainment COO Olivier Garneau reveals that Vidic has volunteered as Subject 2 for his Animus Project, where he relived the life of an ancestor in 18th century Hungary and of one of Joan of Arc's executioners in 1431.

Lucy Stillman (voiced by and modelled on Kristen Bell)[3] (1988–2012) was a member of the Assassin Order and a genetic memory researcher for Abstergo Industries' Animus Project. During her infiltration of Abstergo, she reported information on the company's recent activities to the Assassins. However, Lucy's lengthy separation from the Assassin Order and her distrust for William Miles led to her secret defection to the Templar cause prior to 2011. When Desmond Miles was brought into Abstergo for testing, Warren Vidic instructed Lucy to earn Desmond's trust, so that the Templars could ultimately find the Piece of Eden located within his memories, and use it for their Eye-Abstergo satellite. As Desmond's condition began to worsen however, Warren and Lucy turned to more drastic measures, and formulated Project Siren. Lucy was ordered to take Desmond somewhere he would feel safe, and the two Templars orchestrated Desmond's and her escape from Abstergo. Lucy carried out her orders, and relocated Desmond to a hideout to join his fellow Assassins Shaun Hastings and Rebecca Crane. After a month, and another relocation to the ruined Villa Auditore in Monteriggioni, the Assassin team discovered the location of an Apple of Eden, which was inside the Colosseum Vault. When Desmond's hand made contact with the Apple, his body was possessed by Juno, a member of the First Civilization. With her knowledge of Lucy's true allegiance, Juno forced Desmond to stab and kill Lucy with his Hidden Blade.

Abbas Sofian (voiced by Nolan North in Assassin's Creed and by Yerman Gur in Assassin's Creed: Revelations) (1166–1247) was a Syrian Assassin during the Middle Ages, and the Mentor of the Levantine Assassins from 1227 until his death. His leadership over the Levantine Brotherhood showed a period of corruption and disregard for the Order's Creed, resulting in the decline of their castle in Masyaf and its villagers fearing and secretly loathing the Assassins. Raised as an Assassin from birth alongside Altaïr Ibn-La'Ahad, the two became best friends during their childhood. However, after Altaïr revealed to Abbas that his father, Ahmad Sofian, had killed himself instead of leaving the Order as Abbas had believed, he labelled Altaïr a liar, and developed an intense hatred for him that would span a lifetime. After Altaïr killed their former Mentor, Rashid ad-Din Sinan (Al Mualim), Abbas stood against Altaïr, who desired to become the Order's new leader. However, he eventually repented, and kept a low profile during Altaïr's time as Mentor. When Altaïr and his family left for Mongolia to deal with the threat led by Genghis Khan, Abbas staged a secret coup d'état against the Order, killing Altaïr's younger son, Sef Ibn-La'Ahad, and framing Altaïr's best friend and right-hand man, Malik Al-Sayf, who was thrown into prison. With Sef and Malik out of the way, Abbas held the most power over the Order and so he implemented a council into the Brotherhood, with himself as its head. Eventually, he also disbanded the council, usurping the title of Mentor and becoming the sole leader of the Levantine Assassins. Under Abbas' leadership, the Order declined and became corrupted. Additionally, Abbas spent the majority of his time hiding in Masyaf's fortress, fearing for his life. Eventually, after Altaïr returned from his exile, the Assassins joined him once again, and Abbas was killed by one of Altaïr's newest weapons

Abu'l Nuqoud (voiced by Fred Tatasciore) Damascus' Saracen merchant king. Invited many citizens living in Damascus to a grand party, where attendants received goblets they used to get wine from a wine fountain he set up in the partying area; poisoned wine that is.

Malik Al-Sayf (voiced by Haaz Sleiman)[3] (1165–1228) is a character in Assassin's Creed. Malik Al-Sayf was a member of the Assassin Order in Syria during the High Middle Ages. Raised to be an Assassin, Malik learned the fighting arts that struck fear into the hearts of their enemies. He was an excellent swordsman and a devoted acolyte to the Creed, as well as a caring adult figure for his brother, Kadar. He was once a rival of Altaïr, whom he often berated for disobeying the tenets of the Assassin's Creed. His jealousy turned to hatred when Altaïr ignored the tenets of the Creed, endangered his companions' lives during the events of Solomon's Temple, and subsequently botched a mission, resulting in the death of Malik's brother (Kadar A'Sayf) as well as the injury and subsequent amputation of Malik's arm. Malik retrieved the Templar treasure that Altaïr had failed to find, and delivered it to Al Mualim. No longer able to operate as an Assassin, Malik was made the bureau leader of the Jerusalem Assassins. At first he was bitter towards Altaïr, but over time he comes to forgive him and acknowledges his own fault in his brother's death. When Altaïr returns to confront Al Mualim, Malik supports him, distracting the indoctrinated Assassins while Altaïr faces Al Mualim. After Al Mualim's death, Malik rushes to Altaïr's side as the piece of Eden becomes activated. Altaïr made Malik his close second-in-command after becoming the Mentor. As such, Malik was eventually appointed as a temporary leader in Altaïr's absence. Sometime after this, a hated rival of Altaïr's, Abbas Sofian, imprisoned Malik in Masyaf's dungeons for close to two years with false charges of murder, and had him beheaded when Altaïr returned from his quest across the Middle East. Malik's death occurred during 1228. He is not seen again for the rest of the series, but he is written off in Assassin's Creed II. Altaïr makes mention of his death at the hands of Abbas in Assassin's Creed: Revelations.

Maria Thorpe (voiced by Eleanor Noble) (1160–1227) was an English noblewoman who became a Templar steward, and later the wife of Altaïr. As a child, Maria rejected the gender norms of her time, ultimately being disowned by her parents after annulling her arranged marriage. Seeking to fight in the Third Crusade, she disguised herself as a man and attempted to join the Templar order. The Templar master, Robert De Sable, quickly saw through her ruse, but was impressed by her deterimination and hired her as his steward. Eventually, Robert deduced that Altaïr would be coming for him, so he had Maria serve as a decoy to buy him time. Though she expected Altaïr to kill her, he instead spared her, but warned her not to follow him. After Robert was assassinated, Maria becomes obsessed with revenge. She tracked Altaïr to Acre, but he bested her again, and this time took her prisoner. She escapes in an attempt to join the Templar's new leader, Armand Bouchart, but because of her failure to kill Altaïr, and her "miraculous" escape from him, Bouchart perceives her to be either incompetent, or worse, a traitor to the Order. He places a bounty on the heads of both her and Altaïr, forcing them to work together. They fall in love after Bouchart's defeat. Maria renounces the Templars and becomes Altaïr's wife, bearing them two sons, Darim and Sef. Maria joins Altaïr and Darim to Mongolia in a bid to halt the advance of Genghis Khan. While they are away, Abbas murders Sef. Maria and Altaïr confront Abbas, and Maria is killed in the ensuing fight. Her last words to her husband were "Strength, Altaïr..."

Garnier de Nablus (voiced by Hubert Fielden) is a French noblemen, tenth Grand Master of the Knights Hospitalier in Acre. He experimented and tortured innocent people and lunatics he picked up off the streets of Acre, claiming they were his children and that he was helping them. He transformed Acre Fortress in an hospital and he was killed by Altair inside it.

Ibn Jubayr (voiced by Fred Tatasciore) is a Damascus' Saracen chief scholar and leader of "The Illuminated." He intended to have all texts in Damascus burned, claiming that these texts led people astray. He was killed by Altair during one of his public purge.

Rashid ad-Din Sinan, commonly known as Al Mualim (Arabic: "The Teacher") (voiced by Peter Renaday)[3] or The Old Man of the Mountain, is based on the real-life Rashid ad-Din Sinan, the Iraqi-born Hashshashin leader in the Syrian town of Masyaf. He appears in Assassin's Creed and Assassin's Creed: The Secret Crusade. He was the Assassin's Mentor who secretly led a double life as a Templar; he was also seen as a wizened old man whom Altaïr saw as a father figure. After Altaïr broke all three tenets of the Assassin's Creed in one mission, Al Mualim reduced Altaïr to the rank of an initiate, and gave him a list of nine men to kill in order for Altaïr to redeem himself and bring an end to the Crusade. In fact, Altaïr was killing "rival" members of the Templar Order so that Al Mualim could keep the Piece of Eden for himself. Altaïr eventually assassinates the ninth target, Robert de Sablé, but with his dying breath, de Sablé tells Altaïr of Al Mualim's true plans. Altaïr returns to Masyaf to find that the citizens are being controlled by the Apple, wielded by Al Mualim. Al Mualim himself broke all three tenets of the Creed, leading to a fight between himself and Altaïr, and after a lengthy battle Al Mualim is killed by his student and Altaïr takes possession of The Apple. Al Mualim is back in Assassin's Creed: Revelations in one of the memories of Altaïr. In the memory, Altair burns Al Mualim's body on a pyre, ensuring that he can never return. This is a move that unnerves many of the other Assassins, but eventually Altaïr is forgiven and accepted as the new master.

Richard I of England (voiced by Marcel Jeannin) (1157–1199), commonly known as Richard the Lionheart, was the King of England from 1189 until his death in 1199. He was the second monarch of the House of Plantagenet, being also the commander of the Crusader army during the Third Crusade, and was considered a great military leader and warrior. Upon his death, he was succeeded as King of England by his younger brother, John. Under Richard, the Crusaders eventually reached Arsuf, where they engaged Saladin's army. Here they were approached by Altaïr. Altaïr then claimed that Robert de Sablé, Grand Master of the Knights Templar and one of the generals working alongside Richard, intended to betray the king. However, Robert insisted that Altaïr's story was merely a ruse to keep Richard from interfering in the Assassin's mission. Unsure on who to believe, Richard left the decision in the hands of God, declaring that Robert and his Templars were to fight Altaïr in a trial by combat. Altaïr proved the victor, and so Richard accepted the Assassin's version of events.

Robert de Sablé (voiced by Jean-Philippe Dandenaud) appears in Assassin's Creed and Assassin's Creed: The Secret Crusade. De Sablé was a French lieutenant under Richard the Lionheart, and Grand Masters of the Knights Templar during the Third Crusade. Born into a powerful family, Robert de Sablé was immersed from birth into nobility, though events during de Sablé's life are difficult to pin down; at some point he became lord and ruler of Briollay, France. Subsequently, de Sablé entered into the order of Knights Templar and reigned as their "Grand Master" from 1191 to 1193. During the Third Crusade, de Sablé and the Knights Templar laid siege to the city of Acre, which soon fell; throughout August 1191, they also recaptured many fortresses and cities along the Palestinian coast, which had been lost previously. Grand Master of the Knights Templar, de Sablé follows a goal similar to the Assassins (in that he is attempting to end the war in the Holy Land). Al Mualim has no objections against him ending the Crusade, but is more against the way de Sablé attempts to do so—the Assassins would have people find peace themselves, but the Templar would force their "peace" onto others and attempt to control them.

Sibrand (voiced by Arthur Holden) is a German Crusader and the first Grand Master of the Teutonic Order in Acre. He intended to have all ships submitted to him for him to use in a blockade against King Richard, but he was killed on his personal ship by Altair.

William V, Marquess of Montferrat (voiced by Harry Standjofski), also known as Guglielmo V del Monferrato in Italy, was an Italian Crusader and Richard the Lionheart's regent in Acre. He planned to kill Richard and give Acre to his son, making his plans inside Acre's Citadel.

Shaun Hastings (voiced by and modelled on Danny Wallace)[6] (b. 1985) is a cynical, condescending and pessimistic member of the Assassin's team, specializing in research and information handling. Through conversations in Assassin's Creed II, Desmond learns that Shaun was interested in conspiracies from an early age, and developed a love of history. Though he was unaware of the existence of modern-day Templars and Assassins, he realized that there was some power-struggle going on. Shaun attempted to tell others about his findings, not realizing that the Templars would try to silence him. Fortunately, Rebecca Crane apparently "rescued" Shaun and recruited him into the Order. This is mentioned as being unconventional, as most Assassins were usually born into the Order, not recruited. In 2013, Shaun infiltrated Abstergo by working as a coffee vendor at their Entertainment subsidiary in Montreal, in order to find out what happened to Desmond's body. A year later, under the alias "Deacon", Shaun and Bishop enlisted the help of a Helix player in order to locate the remains of a Sage during the French Revolution.

Rebecca Crane (voiced by and modelled on Eliza Schneider)[7] (b. 1984) is a member of the Assassin Order and the creator of the Animus 2.0, which she affectionately referred to as "Baby". She was a member of Lucy Stillman's team, which concentrated on acquiring an Apple of Eden before Abstergo Industries, as well as training Desmond Miles through the use of the Bleeding Effect. She provided technical support for the other members of the Assassin Order alongside her fellow Assassin, Shaun Hastings. After Desmond's death, Rebecca worked with Shaun to infiltrate Abstergo Entertainment as a courier. In 2015, Rebecca and Shaun headed to London with fellow Assassin Galina Voronina in order to locate and extract the Shroud of Eden. Despite this, their efforts fail, and Rebecca is wounded when she takes a shot meant for Shaun. Nevertheless, the trio manage to escape after Galina dispatches most of the Abstergo agents surrounding them.

Claudia Auditore (voiced by Angela Galuppo)[8] (1461-unknown) was a Florentine noblewoman during the Renaissance, and a member of the Assassin Order. She was the sister of Ezio Auditore, who became the Mentor of the Italian Assassins. In 1476, Claudia became the financial accountant of the town of Monteriggioni, which was under the leadership of her uncle Mario Auditore. The town flourished with Claudia's organization and the money made from Monteriggioni's shops and organizations, which had been opened once more. In January 1500, Monteriggioni was besieged and largely destroyed by the Borgia army, under command of Cesare Borgia, leaving the Auditore homeless. Against her brother's desire for Claudia and her mother Maria to go to their hometown of Florence, Claudia and Maria followed Ezio to Rome, where Claudia became the Madame of the Rosa in Fiore, the city's most popular brothel. Eventually, after proving herself to be a worthy fighter to her brother, Ezio inducted Claudia into the Order of Assassins. The following year, Claudia was captured by Borgia die-hards, and subsequently saved by Ezio and Niccolò Machiavelli. During this time, she stepped down as the Madame of the Rosa in Fiore, and stayed in Florence with her friend Paola, in order to recover until 1507. Claudia was given temporary control of the Italian Assassins in 1510, when her brother left on a journey to the Middle East to find the library of the legendary Altaïr Ibn-La'Ahad, which was said to be in Masyaf. She held this position until her brother returned in late 1512, after which Ezio resigned from the Order and assigned a successor.

Cristina Vespucci (voiced by Amber Goldfarb)[9] (1459–1498) was a well-known Florentine beauty, and a favorite of painters; most notably Sandro Botticelli, who used her as a model for several of his paintings. She was introduced in Assassin's Creed II, though most of her story is told during the "Repressed Memory Sequences" from Brotherhood. She was cousin of the Italian explorer, financier, navigator and cartographer Amerigo Vespucci. She was a first love interest of Ezio Auditore da Firenze. When Ezio had to leave for Monteriggioni, he asked Cristina to come with him. She refused though, as she didn't want to leave her family behind. Accepting her decision, Ezio gave her a pendant to remember him. In 1478, Cristina married Manfredo Soderini, but more because of her father's will than her own. Just one day before their wedding, Ezio returned to Florence and visited Cristina, unaware of the direction her life had taken. Cristina told Ezio she didn't expect him to return and reveals her engagement. Even though he is heartbroken, it is Ezio who appeals to Manfredo's conscience, telling him to stop gambling and start being a good husband. After Lorenzo de' Medici's death in 1494, Girolamo Savonarola takes control of Florence and starts the Falò delle vanità (Bonfire of the Vanities). Because of their rich fineries, the home of Cristina and Manfredo was attacked by the fanatics following Savonarola. Ezio came to her aid, but she was already mortally wounded. Cristina showed him the pendant he had given her twenty-two years ago, and said she wished for another chance with him. She died in his arms.

Caterina Sforza (1463 – 1509) was the Countess of Forlì and Imola, and the daughter of Galeazzo Maria Sforza, the Duke of Milan. She was engaged to Pope Sixtus IV's nephew, Girolamo Riario, at only 10 years of age, and consummated the marriage at 14. As countess of Forlì, she became a strong ally of the Assassin Order, meeting the Assassin Ezio Auditore da Firenze after her husband Girolamo had her trapped on a small island in Romagna. Eight years later, Caterina had her husband killed by the Orsi brothers after finding out that he was working for the Templars, along with being a poor husband. Offering to keep the ancient artifact, the "Apple of Eden", safe in Forlì, Caterina and the Assassins Ezio and Niccolò Machiavelli found the city taken by the Orsi brothers, who were hired by the Templars. Retaking the city, Ezio saved Caterina's children from the Orsi and killed the two brothers, though the Apple was eventually taken from him by a mysterious monk. Caterina later traveled to the Italian Assassins' headquarters in Monteriggioni, requesting for aid against Cesare Borgia's army. Unexpectedly, Monteriggioni was besieged by Cesare the next day, causing the town to be destroyed and Caterina to be captured. The next year, Caterina was transported to the Castel Sant'Angelo in Rome to be jailed. However, her imprisonment lasted not even a day, as Ezio Auditore infiltrated the Castel, with the primary aim of killing Cesare and Rodrigo Borgia. With the two away from the Castel, however, Ezio decided to free Caterina with a key taken from Lucrezia Borgia, and a few weeks later, Caterina returned to Florence to await the restoration of her lands.

Cesare Borgia (voiced by Andreas Apergis) appears in Assassin's Creed: Brotherhood. Cesare Borgia (c. 1475–1507) was the son of Rodrigo Borgia. He was naturally ambitious and aggressive; at age 18, when he sought his brother Juan Borgia the Younger's position as Captain General of the Papal Army (Cesare was a Cardinal at the time), he had his brother assassinated. After leading the army to many victories throughout Italy, Cesare developed extreme sadism and a lust for conquest, having the primary goal to unify all of Italy and conquer all of Spain. In effect, he and his father were continually at odds. Near the end of his life he was imprisoned before escaping and raising an army for a last stand before being thrown off a wall by Ezio, ending his life.

Girolamo Savonarola (1452 – 1498) was a Dominican friar and the leader of Florence from 1494 to 1498. Despite having great ambitions, most men found him lacking the ability to be a great leader. In 1488, Savonarola managed to obtain an Apple of Eden. Recognizing its powers, he used it to instigate the Bonfire of the Vanities in Florence; hoping to cleanse the city of everything he regarded as evil, such as art and wealth.

Leonardo di ser Piero da Vinci (voiced by Carlos Ferro) (1452–1519), better known as Leonardo da Vinci or simply Leonardo, was an Italian Renaissancepolymath. His genius, perhaps more than that of any other figure, epitomized the Renaissance humanist ideal. His lifelong friendship with Ezio Auditore begins when Ezio's mother, Maria, has her son help Leonardo move some paintings from his new workshop. Leonardo comments that his current work lacks purpose, but Maria is confident that he will go on to do great things. After Ezio's father and brothers are murdered by Rodrigo Borgia's men, Ezio brings to Leonardo two objects from his father's study: a broken hidden blade, and a page from the mysterious Codex written by Altaïr. Leonardo repairs the blade and deciphers the codex, fascinated by the technological secrets it alludes to. As Ezio continues his Assassin training, Leonardo makes several upgrades to Ezio's weapons and armor, including a poison blade, a gun, and a poison dart launcher. Some time after Ezio foils the Pazzi conspiracy, Leonardo receives a commission from a Venetian noble, prompting him to set up a new workshop in Venice. As Ezio had his own business in the city, he accompanied him. He developed an interest in flight, and invented an experimental flying machine, which Ezio used on one of his missions. After seeing the Apple of Eden, Leonardo marveled at the futuristic visions it showed him. He then developed an interest in the findings of Pythagoras, and was eventually able to create a map leading to a temple built by his followers. Eventually, Leonardo was blackmailed by Cesare Borgia into designing Templar war machines. He continued to support Ezio, in secret, until he was kidnapped by a third party, the Cult of Hermes, who wanted him to lead them to the Temple of Pythagoras. He is eventually rescued by Ezio, and despite his injuries, explores the Temple, fascinated by the numeric codes found within. Although Leonardo does not appear in Assassin's Creed: Revelations, the novelization describes him dying peacefully at his home in Amboise, with his friends (including Ezio, who is now retired from the Assassins) at his side. In Assassin's Creed III, the Darvenport Homestead's residential carpenter obtained some of Leonardo's blueprints and, at Connor's request, build a prototype replica of the original flying machine.

Lorenzo de' Medici (1449 – 1492) was an Italian statesman and de facto ruler of the Florentine Republic during the Italian Renaissance. Known as il Magnifico (the Magnificent) by contemporary Florentines, he was a diplomat, politician and patron of scholars, artists, and poets. His life coincided with the high point of the early Italian Renaissance; his death marked the end of the Golden Age of Florence. The fragile peace he helped maintain between the various Italian states collapsed with his death. Lorenzo de' Medici is buried next to his brother Giuliano in the Medici Chapel in Florence. Secretly, Lorenzo de' Medici was an ally of the Assassins, particularly the House of Auditore. Lorenzo worked closely with Giovanni Auditore da Firenze, a close friend and ally, against the corrupted Borgia. After Giovanni's death, Lorenzo was saved by his son Ezio during the Pazzi conspiracy. This caused a long-term relationship between them, with Ezio carrying out several assassination contracts in cities such as Forlì and Venice for Lorenzo.

Lucrezia Borgia (voiced by Liane Balaban) (April 18, 1480 – June 24, 1519) appears in Assassin's Creed: Brotherhood. She is the daughter of Rodrigo Borgia and the sister of Cesare Borgia. She is first seen in a portrait in Assassin's Creed II as a little girl with brown hair. She appears in Assassin's Creed: Brotherhood as a woman, her hair now blonde. She is shown to have incestuous affairs with both her father and her brother, the latter of whom tells her that she will be his queen. She has secured a political advantage for her family by marrying many powerful nobles and then having them disgraced or killed soon thereafter. She grows tired of Cesare's womanizing ways, however, and plans to leave the Vatican. Despite this, she still warns her brother of the poisoned apple he received from Rodrigo. In turn, Cesare beat her until she revealed the Piece of Eden's location. Disillusioned by his actions, she helped Ezio find said Piece of Eden and left the Vatican behind. Her in-game portrait reveals that she and her husband fled Rome and relocated to Ferrara where she encountered Ezio again in 1506 while he was looking for the first da Vinci painting, Annunciation.

Pope Alexander VI (voiced by Manuel Tadros) appears in Assassin's Creed: Lineage, Assassin's Creed II, and Assassin's Creed: Renaissance. Cardinal Rodrigo Borgia (Italianized name) (January 1, 1431 – August 18, 1503), born Roderic Llançol, later Roderic de Borja i Borja, was the leader of the Knights Templar during the Renaissance in Italy. He was elected Pope from 1492 to 1503 as Alexander VI. His enemies called him "The Spaniard" (due to his Spanish origins) while his Templar followers called him "Maestro" ("Master" in Italian and "teacher" in Spanish). He was one of the most controversial of the Renaissance popes, and his surname (Italianized as Borgia) became a byword for the debased standards of the papacy of that era. Borgia was elected Pope in 1492 and established his power in Rome; his true intent, however, was to gain access to the vault that lies under the Vatican, where, according to Borgia, God Himself rested. Borgia appeared as a minor character in Assassin's Creed: Brotherhood, which takes place in 1503, the last year of his papacy. He is killed after he is murdered by Casare, who is poisoned by Rodrigo only seconds before.

Niccolò di Bernardo dei Machiavelli (voiced by Shawn Baichoo) (1469–1527) or simply Niccolò Machiavelli, is a supporting character of Assassin's Creed II and Assassin's Creed: Brotherhood. He was an Italian philosopher and writer, and a member of the Assassin Order. With the Assassin Order, Niccolò primarily worked with his ally Ezio Auditore da Firenze, and helped him with driving the Orsi brothers from Forlì and with removing the monk Girolamo Savonarola from power in Florence to obtain the "Apple of Eden" from him. Two years later, Machiavelli took up the position of leader of the Italian Assassins. Once again joined by Ezio, they fought against the corrupted Borgia family, which ruled over Rome.

Clay Kaczmarek (voiced by Cam Clarke in Assassin's Creed II and Assassin's Creed: Brotherhood and by Graham Cuthbertson in Assassin's Creed: Revelations) (1982-2012) also known as Subject 16 of the Animus Project, was a member of the Assassin Order during the early 21st century. Born into a family of engineers, Clay faced psychological problems due to developmental disorders and his father's neglectful attitude. Because of this, Clay ran into the Assassins while looking for acceptance. As a member of the Assassin Brotherhood, Clay's most important mission was to infiltrate Abstergo Industries as a subject of the Animus Project, in order to obtain more information on the Animus. Labeled Subject 16, Clay was forced to relive the memories of his ancestors via his genetic memory. When Clay found out that his teammate, Lucy Stillman – who infiltrated Abstergo years before and was tasked with getting Clay out of Abstergo safely – had abandoned their cause to join their enemies, the Templars, Clay had to spend days and hours of sessions inside the Animus. Since it left his mind incapable of separating his own personality from those of his ancestors', Clay became mentally unstable to the point that he ultimately committed suicide. After his alleged suicide, Clay continued to exist as an AI recreation of his personality within the Animus. As such, he was able to manipulate much of the Animus' programming from within to help his successor in the Animus Project, Desmond Miles.

Sofia Sartor (voiced by Anna Tuveri) (1476-unknown) as a Venetian-Ottoman bookshop owner, and a literary enthusiast. Though born in the city of Constantinople, Sofia was forced to leave for Venice in 1499, when the Ottoman-Venetian war began. Sofia eventually returned to her birthplace in 1507, in order to run a bookstore at the old Polo trading post. In 1511, she met the Assassin Ezio Auditore da Firenze, the man who would eventually become her husband, and the father of their two children. In this regard, she is an ancestor to Desmond Miles through the paternal line. A polylingual, multicultural, and intelligent woman mature beyond her years, Sofia had a passion for literature, which fueled her decision to run her bookstore, in the hopes of sharing the democratizing effect she felt in books with others.

Tarik Barleti (voiced by JB Blanc) (1470-1511) was a character in Assassin's Creed: Revelations. Tarik Barleti is the captain of the Janissaries, the personal guard of the Ottoman Sultan. Tarik was born to a Christian Albanian family, and he was taken at a young age to be trained to fight. He is a close friend of Prince Selim I. Ezio thought that Tarik to be a Templar because of his behavior, and he was selling weapons to Manuel Palaiologos, a known Templar. Ezio decided to act on his theory, and he struck Tarik down. In his final moments, Tarik revealed that he planned to attack Manuel's Byzantine Templars in Cappadocia. He made a final request to Ezio to stop the Templars. Ezio honored it. Tarik is one of the few assassination targets that is unaffiliated with the Templars or Assassins.

Vali cel Tradat (unknown-1511) was a Wallachian noble, former Assassin and member of the Templar Order. Having served the Assassin Order for around a decade, Vali left the Assassins after they made a truce with the Ottomans; the same people who had humiliated his people, conquered Wallachia, and murdered his secret idol Vlad Tepes. Feeling betrayed, he joined the Templars, striving for vengeance against the Assassins and Ottomans.

Yusuf Tazim (voiced by Chris Parson) is a character in Assassin's Creed: Revelations. He is an easygoing Master Assassin, and leader of the Ottoman Assassin Brotherhood, rising to the post sometime before 1511 AD. That year, he met Ezio Auditore, and he introduced Ezio to Constantinople and the Ottoman hookblade. Ezio describes Yusuf as "affable". Yusuf often talks with humor, poking fun at Ezio or at Italians in general. Furthermore, he is an expert at crafting bombs and at combat. When Ezio returns from Cappadocia, he finds Sofia Sartor's shop in ruins and Yusuf murdered at the hands of Ahmet, a note knifed to his back. The message on the note is never revealed.

Manuel Palaiologos (voiced by Vlasta Vrána) (1454-1512) is the heir to the lost Byzantine Empire throne. Manuel is a major Templar Leader and appears in Assassin's Creed: Revelations. He is conspiring in taking back Constantinople with the help of Ahmet, heir to Sultan Bayezid II of the Ottoman Empire. He is assassinated by Ezio in the Byzantine-controlled city of Cappadocia.

Ahmed Muhiddin Piri (c. 1467-c. 1554), better known as Piri Reis, was a famed admiral and cartographer. He was also a member of the Assassin Order and served the Assassins as a technician and intelligence gatherer, primarily providing them with materials and methods for crafting bombs. He was a trusted friend of the Turkish Assassin leader Yusuf Tazim, and also later befriended Ezio Auditore.

Şehzade Ahmet (voiced by Tamer Hassan) appears in Assassin's Creed: Revelations. Ahmet (1465-1512) was the eldest living son of Bayezid II and brother to Selim I. Sharing many characteristics with his father, he was believed to be the perfect candidate for the sultan's heir, even more so when he served as governor of Amasya. However, many of the Ottoman Empire (The Janissaries in particular) deem him to be an unfit ruler in times of war and times of peace. Furthermore, they do not see him as a traditional Sultan and thus do not support him. He sets up the unsuccessful ambush and capture of Prince Suleiman by Byzantine Templars, intending to have him rescued, subsequently making Ahmet appear to be a brave hero. His plans fail, but while Ezio is away in Cappadocia, Ahmet takes Sofia Sartor hostage and demands Ezio exchanges the Masyaf key for her. After the exchange Ahmet flees the city and Ezio follows him with Sofia. After Ezio catches up with him, Ahmet's brother Selim arrives. Selim proclaims his authority as the new Sultan and kills his own brother by strangling him and then tossing him off of a cliff. (Sehzade is the Turkish form of the title "Prince" and refers to a son or male line descendant of an Ottoman Sultan).

Suleiman I (1494 – 1566), was the tenth Sultan of the Ottoman Empire from 1520 until his death. He was the son of Selim I. Suleiman I was considered one of the Empire's greatest rulers, and was known in the West as Suleiman the Magnificent, as his reign engineered the Golden Age of Constantinople. He was also known by his fellow Turks as Kanuni or the Law Giver. Suleiman became embroiled in the struggle between the Templars and Assassins during 1511. After a failed kidnap attempt by Byzantine Templars working for Manuel Palaiologos, Suleiman befriended the Italian Assassin and Mentor, Ezio Auditore, who encouraged Suleiman's progressive attitude for peace in the Ottoman Empire.

Flavia Auditore (voiced by Angela Galuppo) is a character in the short-film Assassin's Creed: Embers. She was the daughter of Ezio Auditore da Firenze, and the next ancestor to Desmond Miles through that bloodline. She was present with her mother in Florence, when her father died of a heart attack.

Ratonhnhaké:ton (alias: Connor; 1756 – unknown) was an Anglo-Kanien'kehá:ka Assassin who struggled to protect the Haudenosaunee as he fought for the Patriots during the American Revolution War. Trained by Achilles in the mid eighteenth century. He mastered the "Tomahawk", a short axe, and rope darts. Known for assassinating William Johnson, Benjamin Church, John Pitcairn, Thomas Hickey, Charles Lee, Kanen'tó:kon and Haytham Kenway (His own father). He developed and maintained a strong working relationship with Commander Washington, even after learning Washington's responsibility for his mother's death. He is an ancestor of Desmond Miles through the paternal line. Connor is also the son of Haytham Kenway and the grandson of Edward Kenway. He appears in Assassin's Creed III and Assassin's Creed III: Liberation. He is voiced by Noah Watts.

Haytham E. Kenway (1725 – 1781) was a British Templar and eventual first Grand Master of the Templar Order's Colonial Rite, reigning from 1754 until his death. He is the son of Edward Kenway and the father of Ratonhnhaké:ton, and an ancestor to Desmond Miles, all of whom were members of the Assassin Order. He is the playable character in the first three sequences of Assassin's Creed III and an antagonist of the second half of the story. He also is a key character in Assassin's Creed Rogue. He was assassinated in 1781, trying to save his Templar friend Charles Lee by Connor, his own son. He is voiced by actor Adrian Hough.

Io:nhiòte was the daughter of the Assassin Ratonhnhaké:ton, granddaughter of the Templar Haytham Kenway, and great-granddaughter of the pirate Assassin Edward Kenway. The youngest of Connor's three children, she possessed the rare ability of Eagle Vision.

Daniel Cross (1974–2012) was a member of the Templar Order and a sleeper agent tasked with the infiltration of the Assassin Brotherhood, in order to assassinate its Mentor. Daniel was the grandson of Innokenti Orelov and the great-grandson of Nikolai Orelov, a prominent member of the Russian Assassin Brotherhood. Daniel was killed by Desmond Miles in Assassin's Creed III. He is voiced by Danny Blanco-Hall.

William "Bill" Miles (voiced by and modelled on John de Lancie) (b. 1948) is the father of Desmond Miles and de facto leader of the entire Assassin Order during the events of the games, having risen to the position following the death of the Mentor in 2000. He raised Desmond in the ways of the Assassins, teaching him about the order and what they fought for, his training often scared Desmond which ultimately led him to run away from his parents. William's first appearance is in 'Revelations' where he talks with Rebecca and Shaun when Desmond is in the Animus Black room explaining Desmond's importance to the Assassin's on his ability to wield the Apple properly. He questions Rebecca and Shaun if Desmond and Lucy were close and he felt sad about Lucy's death claiming that she was 'a remarkable woman'. He is the first person to greet Desmond after recovering from his coma. In Assassins Creed III, William and his team arrive at the Grand Temple. William is captured by Abstergo agents in Egypt when attempting to retrieve a power cell needed to activate the Vault within the Grand Temple and is taken to their facility in Italy. Desmond eventually infiltrates the facility, rescues William and retrieves the power cell.

Achilles Davenport: (voiced by Roger Aaron Brown) (1710-1781) was a Master Assassin and a mentor for a span of time during the French and Indian War and the American Revolutionary War. He appears in Assassin's Creed III and Assassin's Creed: Rogue. Achilles, born in the Caribbean, was the last recruit in the mid-1730s of Mayan Grand Master Ah Tabai. He was the Mentor of the Colonial Brotherhood of Assassins from 1746 until 1763. A man of Caribbean and British descent, Achilles' tenure as leader of the Colonial Assassins saw the Brotherhood strengthen greatly until 1763 when the Brotherhood collapsed and ultimately disbanded as a result of his mismanagement that very nearly destroyed the world which Haytham Kenway shot Achilles in the leg as punishment for endangering innocents after his former student Shay Cormac convinced the latter to spare his life. Living in exile in his manor, Achilles abandoned the Assassin ideology until his meeting with the young Ratonhnhaké:ton, Haytham's son, whom he started to train on the boy's behest. Achilles spent his final years guiding Ratonhnhaké:ton, and this tutelage led to his apprentice's important role in the American Revolution and the extermination of the colonial Templars. In 1781 Achilles died quietly in his sleep. When Connor discovered his body, he also uncovered a note addressed to him in Achilles' handwriting. Written within, Achilles apologized for never being able to say goodbye to Connor properly, and also thanked him, expressing pride and gratitude in Connor for the community he had raised at the Homestead. A funeral service was held on the hill, behind his manor overlooking the sea, next to the gravestones of his wife and son; this event was attended by all of the inhabitants of the Homestead that Connor had brought together. While others laid roses on his coffin, wrapped in the Davenport Homestead flag, Connor placed a feather on the casket, symbolizing the bond they shared as Assassins.

Kanen'tó:kon (voiced by Akwiratékha Martin) (1756-1778) was a member of the Kanien'kehá:ka nation of the Mohawk Valley (where the Kanien'kehá: ka were also members of the Iroquois Confederacy) and a close childhood friend of Ratonhnhaké:ton. Though he initially advocated for the Kanien'kehá:ka to remain neutral in the American Revolutionary War, he and several other of his village's warriors later began to believe that they could protect their people by siding with the British. In this regard, he was manipulated by Charles Lee into believing that Ratonhnhaké:ton had joined George Washington in a campaign against the Mohawk people, prompting him to attack his former friend when he tried to intervene in a Mohawk ambush on colonial soldiers. Pinned to the ground and vulnerable to a lethal blow, Ratonhnhake:ton was forced to stab Kanen'tó:kon in the neck with his Hidden Blade, killing him.

Kaniehtí:io (/ɡæjɛtˈziːoʊ/; "Gah-yet-ZEE-oh", shortened to Ziio/"Zee-oh", voiced by Kaniehtiio Horn) (1731-1760), or Ziio (informally), was a Kanien'keha:ka (Mohawk) clan woman and the mother of Ratonhnhaké:ton, and an ancestor to Desmond Miles. Kaniehtí:io (1731 – 1760), colloquially known as Ziio, was a Kanien'kehá:ka warrior from the village of Kanatahséton. Unlike her fellow villagers, who preferred to stay neutral to protect their sacred sanctuary, she sought to fight the British encroaching on her people's lands. She gained an ally in the Templar Haytham Kenway, who sought access to the sanctuary. The two shared a brief relationship, which resulted in the conception and birth of a son whom she named Ratonhnhaké:ton. When her son was four, she died during an attack on the village ordered by George Washington; Ratonhnhaké:ton grew up believing Haytham's subordinate Charles Lee was responsible, setting him on the path to join the Assassins.

Oiá:ner was the Clan Mother of the Kanien'kehá:ka village of Kanatahséton. She and the other Kanien'kehá:ka in the village served as protectors of the sacred ground on which both their village and the Grand Temple stood. Oiá:ner was also the keeper of the tribe's "Crystal Ball" (Piece of Eden). In 1769, Oiá:ner showed their Crystal Ball to Ratonhnhaké:ton upon his return from a hunt and allowed him to interact with it. The Crystal Ball forced him into the Nexus, where he communicated with Juno and learned of his destiny with the Assassins. Many years later, the Clan Mother met with an adult Ratonhnhaké:ton and told him of the whereabouts of Kanen'tó:kon and other Kanien'kehá:ka men, who were attempting to attack the Continental soldiers. Some time following Kanen'tó:kon's death, Oiá:ner spoke with Ratonhnaké:ton once more, where she revealed to him that she planned to move their people to the west to avoid the American Revolutionary War, like many of the other nations had been doing. After his unpleasant reaction, she told him that she would do what was best for her people, to which Ratonhnaké:ton left the village. On his return some years later, he found the village deserted save for an old vagabond, who told him that his people had already gone.

Reginald Birch (voiced by Gideon Emery) (1705–1757) was the Grand Master of the British Rite of the Templar Order, and a successful English businessman. He began his dealings at a young age, and by the time he had reached his mid-20s, Birch owned several merchant ships; mainly involved with the dealing of tea to the British American colonies. Later on in his life, Birch would progress to own several businesses around London. Birch met Haytham Kenway, the son of Birch's employer - the Assassin Edward Kenway - at a young age. He recruited Haytham into the Templars after the death of Edward Kenway, personally involving himself with the boy's training. In 1754, after Haytham had retrieved the key to the undisclosed Grand Temple, Birch secured passage to Boston for Haytham to find the First Civilization storehouse and establish a permanent Templar presence in British America. Once Haytham had discovered the truth of Birch's involvement in the kidnapping of his half-sister, Jennifer Scott, and the murder of his father, the Templar stormed Birch's chateau with Jim Logan and Jennifer. Following this, Birch was killed by Jennifer, via a sword that Haytham had embedded into Birch's bedroom door (killing the guard on the other side) prior to a struggle between Reginald and the woman.

Robert Faulkner (voiced by Kevin McNally) (1715-unknown) was an elderly sailor, and Ratonhnhaké:ton's first mate aboard the Aquila. Although he was the fourth generation in his family to become a sailor, he was first to be recruited into the Assassin Order.

Born in 1715, Robert Faulkner is probably the most storied members of the Assassin's navy - though he was never formally named to a position above First Mate.[10]

Robert Faulkner was the fourth generation of his family to be a seaman - but the first in his family to be recruited to work for the Assassins.[10] He started off in the Royal Navy, but finding his career stalled (partly due to his inability to purchase commissions), he left to work for the United Company of Merchants.[10]

In 1753, Faulkner disappears from the historical record, presumed dead - but that's because he joined the Assassins, as first mate of the flagship, the Aquila.[10]

Faulkner was much sought after as a mariner - recruiting him was quite a coup for the Brotherhood.[10] He had a reputation for having a disciplined crew and an almost uncanny knack for avoiding trouble - not to mention predicting the weather.[10] Whether through years of experience, or some other more mysterious ability Faulkner would know when a storm patch of sea was coming in, and could use it to his advantage.[10] Imagine that! A natural weatherman who could accurately predict the weather! The world would never see his like again.[10]

Faulkner served on board the Aquila until it was nearly destroyed in 1768, always deferring when asked to become the captain - he never gave his reasons why.[10] However, his love for the ship was quite touching - he had it towed to its location near Boston, despite doubts that it would ever be seaworthy again.[10] Faulkner could have gone back to the sea aboard another ship, but didn't - he stayed close to the ever-deteriorating Aquila and took to drinking, which is where Connor found him.[10]

Silas Thatcher (1720–1754) was a high-ranking officer of the British Army, and was a prolific slave trader. Silas was responsible for the ransacking of Benjamin Church's house, and his subsequent interrogation, alongside a man called "Cutter". However, shortly after he left, Haytham Kenway and Charles Lee freed their associate, through infiltrating the location Church was kept – after they determined his whereabouts through eavesdropping – and the silent assassination of Cutter and two guards. Later, while posted at Southgate Fort in Boston, he would receive deliveries of prisoners. In 1754, one such convoy, full of Mohawk tribespeople, was commandeered by Haytham and his men as a means to enter the fortification. Shortly after, on discovering the ruse, Silas ordered his men to fire on the Templars, but he was cornered by Haytham and executed by Church, as a means of payback for the latter's previous maltreatment.

Kahionhaténion was a Kanien'kehá:ka, born and raised in the village of Kanatahséton, as a warrior and a hunter during the American Revolutionary War. He was also the brother of Teiowí:sonte. Kahionhaténion helped to defend Kanatahséton from an attack by Washington's troops. After the attack, Kahionhaténion and his brother, Teiowí:sonte, recovered Ratonhnhaké:ton from the village, using the powers granted them by the Red Willow. When Teiowí:sonte was captured by the Bluecoats while trying to free enslaved people from the Frontier, Kahionhaténion asked Ratonhnhaké:ton to help him find and save his brother. Despite this, Kahionhaténion and his brother were killed in battle when Bluecoats followed Ratonhnhaké:ton's tracks back to the cave that the clan were hiding in.

Teiowí:sonte (voiced by Andreas Apergis) was a Kanien'kehá:ka warrior during the American Revolutionary War. He was also the brother of Kahionhaténion. Teiowí:sonte was born and raised in the village of Kanatahséton as a warrior and a hunter.

Benjamin Franklin (voiced by Jim Ward) (17 January 1706 – 17 April 1790) was a Freemason, a noted polymath, and one of the Founding Fathers of the United States. Benjamin wandered around Boston near his store, and was surprised to find Haytham Kenway offer a lending hand, as those living in Boston usually refused to be of any help, and correctly assumed that Kenway was new to Boston. Franklin then charged Kenway with the task of finding stolen pages of his Almanac. Haytham interacted with Franklin, where he proposed his idea of having an older woman as a lover would benefit lives, much to Haytham's amusement. In The Tyranny of King Washington, after gaining control over the area, King Washington put Franklin in charge of running Boston. While under Washington's control, Franklin was still hesitant of the King's decisions, claiming one such order to behead random civilians to be "rather harsh." After being freed from Washington's control by Ratonhnhaké:ton, Franklin sought to help the rebellion take down King Washington.

Charles Lee (voiced by Neil Napier) (1732 – 1782) appears in Assassin's Creed III. Charles Lee was a British soldier, and later, a General of the Continental Army during the American Revolutionary War who allied himself with the Templar Order and its Grand Master, Haytham Kenway. It is implied that Lee sets fire to Ratonhnhaké:ton's village, killing his mother in the process where the then young Ratonhnhaké:ton' vowed to kill him. Many years later, during a meeting with George Washington, they meet again but failed to recognize him until after he gets falsely arrested for counterfeiting along with a Templar called Thomas Hickey. Sarcastically congratulating him for tracking him down, he frames him for a Templar plot to kill George Washington, but the plan is foiled when Washington is saved by Ratonhnhaké:ton'. Years later, residing in Fort George for his "own protection", he is ordered to leave for Boston by Haytham, shortly before the latter's death. Succeeding him as the next Grand Master, he vows to make his tormentor suffer, only to end up being chased by him into a half constructed ferry that's set alight, being saved at the last minute when part of the ship collapses on the Assassin, wounding him in the hip. Using the opportunity to ask him why he continues to, "fight after all his allies have abandoned him", he is told that, "because no one else will", before being shot in the side by him. Fleeing, he's finally tracked down by his pursuer to a tavern in Monmouth County, where they both share a bottle of whiskey, before being fatally stabbed in the chest by the vengeful Assassin. The medallion (the key meant for Desmond) is taken from his body and, shortly afterwards, is buried in the grave of Connor Davenport, Achilles' son and the namesake of Ratonhnhaké:ton.

George Washington (voiced by Robin Atkin Downes in Assassin's Creed III and by Tod Fennell in Assassin's Creed Rogue) (1732-1799) was the Commander-in-Chief of the Continental Army in the American Revolutionary War from 1775 to 1783, and served as the first President of the United States from 30 April 1789 to 4 March 1797. Born in the colony of Virginia in the British colonies of America, Washington joined the Virginian militia during his youth and participated in the French and Indian War with the British Army, fighting against the French and their indigenous allies. During the war, he ordered the burning of the neutral village of Kanatahséton. Due to the lack of progress of his military career in the British Army, Washington instead turned to politics. When the American Revolutionary War broke out, Washington supported the Patriots. Following the creation of the Continental Army, the Continental Congress of 1775 appointed Washington as its Commander-in-Chief. The Assassin Ratonhnhaké:ton, better known as Connor, subsequently protected Washington from the Templar Charles Lee, who was angered that he had been passed over for the position. Though the Continental Army suffered many losses under Washington's command, the aid of Connor and his apprentices managed to gain the army small victories. However, Washington fell out with Connor when the Assassin discovered he was responsible for the attack on his village, Kanatahséton, and signed for a second burning after Lee manipulated Connor's people into siding with the Loyalists. However, the Assassins continued to aid Washington when necessary, such as the investigation of the conspiracy at West Point. After the Continental Army emerged victorious against the British Army in 1781, Washington obtained an Apple of Eden. It gave him a nightmarish vision of what would happen if he were crowned king of the United States with its power in his possession, and so he passed the Apple to Connor for it to be disposed of. This experience strengthened his belief in the Republican Constitution of the United States.

John Pitcairn (voiced by Robert Lawrenson) (1722-1775) was a Scottish born member of the Royal Marine Corps and the Templar Order. He was rescued by Haytham Kenway and Charles Lee while he was being harassed by General Braddock also known as "The Bulldog" who was later killed by Haytham Kenway during a horse chase. Assassinated by Connor during a battle between the Patriots and "Redcoats".

Portrait of Gilbert Motier, Marquis de La Fayette, as a Lieutenant General, in 1791 by Joseph-Désiré Court.

Marie-Joseph Paul Yves Roch Gilbert du Motier, Marquis de La Fayette (voiced by Vince Corazza) (1757 – 1834) often known as simply Lafayette, was a French aristocrat and military officer born in south central France. During the American Revolutionary War, Lafayette served as a Major-General in the Continental Army under George Washington. Blocked by British spies who learned of his support for the Patriots, Lafayette disguised himself as a woman to board a ship to America in 1777. He later recounted this to the Assassin Ratonhnhaké:ton' while camping in Valley Forge that winter. On June 28, 1778, Lafayette led a battalion during the battle of Monmouth, when he was given strange orders by Charles Lee to advance into enemy lines without sufficient numbers. Ratonhnhaké:ton' deduced Lee was trying to make Washington appear incompetent. In 1781, Lafayette visited the Davenport Homestead to help Ratonhnhaké:ton' gather a fleet to breach Fort George in New York. After Ratonhnhaké:ton' gained the French Navy's support, Lafayette met with him and Stephane Chapheau underground and showed them a tunnel into the military district, so Ratonhnhaké:ton' could assassinate Lee in the midst of the bombardment. After the war, Lafayette returned to France and took part in the French Revolution, bringing democracy and radical national reform to France. However, he went into exile because he advocated a constitutional monarchy, instead of the empire that had emerged from the chaos in France.

Samuel Adams (voiced by Mark Lindsay Chapman) (1722–1803) was an American statesman, political philosopher, a Sons of Liberty Patriot and one of the Founding Fathers of the United States. As a politician in colonial Massachusetts, Samuel was a leader of the movement that became the American Revolution. He was also one of the architects of the principles of American republicanism, which shaped the political culture of the United States. During the American Revolutionary War, Samuel met and became a trusted associate of the Assassin Ratonhnhaké:ton', after Achilles Davenport told Ratonhnhaké:ton' to find him on his first trip to Boston. Amongst other things, Samuel taught Ratonhnhaké:ton' how to reduce his notoriety after the Boston Massacre, introduced him to the Mason tunnels underneath Boston, as well as Commander-in-Chief George Washington.

Official Presidential portrait of Thomas Jefferson by Rembrandt Peale, 1800.

Thomas Jefferson (13 April 1743 – 4 July 1826) was one of the American Founding Fathers of the United States, the principal author of the Declaration of Independence and the third President of the United States. During the fight against King Washington's tyranny, Thomas Jefferson was the leader of the New York rebellion. When Ratonhnhaké:ton crashed the Aquila into another ship off the shore of New York City, Washington traveled to the docks to investigate the disturbance. Jefferson seized this opportunity and infiltrated Washington's fortress. He was later quickly overwhelmed and required Ratonhnhaké:ton's assistance in order to retreat. Shaun Hasting claims Jefferson advocated the castration of homosexuals; Jefferson was actually liberalizing the law in Virginia, which previously punished anyone convicted of sodomy with death.

Edward Braddock (1695–1755), nicknamed the Bulldog by his soldiers, was a member of the Templar Order and a general in the British Army. He appears in Haytham's story in Assassin's Creed III, having participated in the plot to kill his father Edward and then acted as a friend and a mentor in the Dutch Campaign. During the French and Indian War, Braddock was the commander-in-chief for the thirteen colonies of British America. He had several soldiers under his command, including John Pitcairn and Charles Lee, whom he rarely saw due to their allegiance to the Templar cause. Over the course of the war, Braddock turned away from the Templar ideals and impeded the Order's goals in the colonies, much to the chagrin of the other members. This disobedience came to a head when Braddock was confronted by Haytham Kenway, Braddock's former friend and fellow Templar, when Haytham attempted to recruit John Pitcairn for the expedition to find a First Civilization storehouse. Braddock was eventually killed by Haytham, in order to gain the trust of Kaniehtí:io, a Kanien'keha:ka woman who promised to show Haytham the location of the storehouse, in exchange for eliminating Braddock. Haytham also took the Templar ring from Braddock's finger, which was later given to Charles Lee upon the latter's initiation into the Templar Order.

Sir William Johnson, 1st Baronet (voiced by Julian Casey) (1715-1774) was an Anglo-Irish official of the British Empire and a member of the Templar Order. During the Seven Years' War, Johnson commanded Iroquois and colonial militia forces. Johnson also assisted in keeping Native Americans committed to the interests of the British. As a member of the Templars, Johnson was in charge of managing the land and property acquired by the Order's Colonial Rite. During a meeting with several clan leaders, Johnson was assassinated by the Assassin Ratonhnhaké:ton.

Antonio de Ulloa (1716–1795) was a Spanish general, explorer, author, astronomer, colonial administrator and the first Spanish governor of Louisiana. A Templar, he was pursued by the Assassin Aveline de Grandpré during the Louisiana Rebellion, but was spared by Grandpré under the promise to go into exile.

Agaté (unknown-1777) was a former slave who, following his escape from jail, became Mentor of the New France Assassin Order in the colonial French Louisiana. He appears in Assassin's Creed III: Liberation. He was the Mentor of the Assassin Brotherhood in Louisiana, operating from his hideout in the Louisiana Bayou. Enslaved from a young age, Agaté came into contact with revolutionary disruptor François Mackandal, who taught him the ways of the Assassins. Following the death of his Mentor in 1758, Agaté traveled to Louisiana, a marked man, and hid in the bayou. Agaté subsequently established the local Assassin guild, recruiting Aveline de Grandpré and Gérald Blanc, whom he trained to be his agents in New Orleans. After the Templars' presence in the bayou notably increased in 1766, Agaté went further into hiding, constructing a hidout deep within the swamp. Although Agaté cared for his students, he was quite secretive in his dealings with them. Due to Aveline's natural impulsiveness and disinclination to follow orders, Agaté clashed with her frequently and eventually began to doubt her loyalty to the cause. Over the years, their mutual distrust of each another caused the two to grow apart. When Aveline returned to Agaté in 1777 to tell him that Louisiana's head Templar had been her own stepmother all along, Agaté attacked Aveline, believing her to have betrayed the Assassins. His student managed to prove victorious, however, causing Agaté, who was overwhelmed with humiliation, to leap to his death from the top of his treehouse.

Baptiste was a former Assassin and voodoo leader, who posed as the deceased François Mackandal and operated in the Bayou surrounding New Orleans. Secretly working for the "Company Man" with Rafael Joaquín de Ferrer, Baptiste aspired to become a formal member of the Templar Order. He was also a personal enemy of the Mentor of the Louisianan Assassins, Agaté. Sometime in 1765, "Mackandal" had amassed a small following, though he sent a letter to one of his acolytes stating that he was "in want of nothing but more men to join his ranks" of his cult for a "sacred ceremony" on the Eve of Saint John. However, this letter was intercepted by the Assassin Aveline de Grandpré, shortly after she had assassinated the troops at one of his bases, a wrecked ship in the marshlands, with the smuggler Élise Lafleur providing her with the information and transport to get there. Aveline and Élise then located Baptiste after she had cleared the man's second base, and spied on his conversation with de Ferrer. There, she learned of his aspirations to poison the nobles of New Orleans, before Aveline faced off against Baptiste in a showdown, proving victorious despite having been afflicted by a hallucinogenic drug by the voodoo leader; she had ingested an antidote beforehand in preparation to face him. He is portrayed by Michael K. Williams in the Assassin's Creed film.[15]

Gérald Blanc was an accountant living in New Orleans during the end of the French and Indian War. At some point in his life, he became an associate of the Assassin Aveline de Grandpré. Later, he came to consider himself as an Assassin even if he lacked the physical training, referring to the Brotherhood as "our Order" when talking with Aveline.

George Davidson (1752–1777) was a slave, whom the Assassin Aveline de Grandpré helped to escape from New Orleans and confined into the care of the American patriots as asked by her step-mother. Later, when Aveline was sent to New York to meet Connor in order to take down the British Officer of the loyalist Ethiopian regiment of Lord North, who had information on the identity of the "Company Man", Aveline shockingly discovered George's allegiance to the Templar Order. George was killed by Aveline, and in his dying breath, he told her that "the answer has been in her own backyard all along", prompting the Assassin to understand that the Company Man was her own step-mother, Madeleine de L'Isle.

Madeleine de L'Isle (1732-1777) was the wife of Philippe Olivier de Grandpré, the stepmother of Aveline de Grandpré. She appears in Assassin's Creed: Liberation. Under the alias of the "Company Man", she was also secretly the supervisor of the Templar activities in the French Louisiana territories.

Rafael Joaquín de Ferrer (1730s-1769) was a Spanish Templar working for the "Company Man", who ran a camp of seemingly escaped slaves in Chichen Itza, where he was conducting archaeological research in order to find an artifact of the First Civilization, the Prophecy Disk. In 1769, de Ferrer was killed by the Assassin Aveline de Grandpré who had infiltrated his labor camp and found the Piece of Eden he was looking for, which he attempted to take from her.

Diego Vásquez was the name of a Templar who was assumed to be the Order's "Company Man" in the French colonies of North America. He was later killed at a soirée by the Assassin Aveline de Grandpré, after having been charmed by her in her Lady attire, as part of her search to uncover the true identity of the Company Man. On his death, however, Vásquez revealed to Aveline that she had been misguided, and disclosed that the Company Man which the Assassin sought was a female in reality. This mystery woman was eventually discovered to be Madeleine de L'Isle, though Vásquez perished before he could give out her name.

Edward James Kenway (1693–1735) was a Welsh-born British privateer-turned-pirate, and a Master Assassin. He is the father of Haytham Kenway, the grandfather of Ratonhnhaké:ton, and an ancestor of Desmond Miles. Edward Kenway appears in Assassin's Creed IV: Black Flag. He is voiced by actor Matt Ryan.

Duncan Walpole (1679–1715) was a Master Assassin of the British Brotherhood of Assassins during the early 18th century. Skilled but arrogant, Duncan later chose to betray his Order and join the Templars of the West Indies. He is killed by Edward Kenway before he could join the order.

Jing Lang (1686–1717) was a Chinese Templar, pirate, and target of Edward Kenway in the early 18th century. The daughter of a rebel, Jing Lang grew up fighting. As a young woman, she developed a talent for negotiation and a knack for languages, as well as a taste for discovery. She broke ties with her family to marry the son of a Qing dynasty general her father opposed, and served as their military adviser. Ultimately for performing so well, she was granted a diplomatic post. Over the next decade she traveled the world, developing a reputation for brutality that preceded her in most conflicts. Using piracy as a cover for her political activities, she banded with any who might serve her ultimate desire for negotiated order. In the West Indies of 1717, she fell under the interest of a rumored cache of treasure, and feigned a romantic relationship with one-time Assassin, Vance Travers, to gain access to it. Under the suspicion of Jing being a Templar, Vance's brother, Upton, ordered Edward Kenway to have them both killed. Edward shortly assassinated her to collect a Templar key.

Josiah Burgess (1689–1719) was an English pirate based in Nassau. Like John Cockram, he had a close relationship with Benjamin Hornigold, one of the leaders of the small pirate republic. After accepting the King's pardon, he was recruited into the Templar Order as a pirate hunter, tracking down and eliminating his former allies. He is killed by Edward Kenway.

Julien du Casse (1682–1715) was a French arms dealer and a member of the Templar Order in the West Indies. He possessed a discreet hideout on Great Inagua to help him carry out his trade and Templar affairs as well being the nephew of the real-life French Privateer Jean-Baptiste Du Casse. An experienced privateer and mercenary, du Casse was introduced to the Order by Laureano de Torres y Ayala. Quickly proving himself to be an asset, du Casse functioned as Torres' hitman, as well as the Caribbean Templars' main supplier of weaponry and ammunition.

William Kidd (c. 1645–23 May 1701) was a Scottish sailor who was tried and executed for piracy after returning from a voyage to the Indian Ocean. Modern historians deem his piratical reputation unjust, as there is evidence that Kidd acted only as a privateer. Kidd's fame springs largely from the sensational circumstances of his questioning before the English Parliament and the ensuing trial. His actual depredations on the high seas, whether piratical or not, were both less destructive and less lucrative than those of many other contemporary pirates and privateers. In Assassin's Creed III, his treasure maps are a part of side missions: Connor finds a map locating his treasure inside Fort Wolcott.

Charles Vane was a British Pirate who was Captain sailing with Anne Bonny and Jack Rackham. In game Charles Vane never agreed with Benjamin Hornigolds decisions to get a pardon and sailed with Jack Rackham and Edward Kenway. Charles Vane ends up in prison and is never seen again.

Anne Bonny (née Cormac; 1702–1743) was an Irish pirate who operated in the West Indies. During the early 18th century, she became an associate of Edward Kenway, and later assisted him closely. Initially a young barmaid at the Old Avery tavern in Nassau, Anne quickly became acquainted with individuals such as "Calico" Jack Rackham and Mary Read, the latter initially posing as James Kidd. Launching her own career as a pirate, she, along with Mary and Rackham, raised a small crew and escaped the island in August 1720, intending to execute a series of swift robberies before retiring with enough gold to live off. However, the entire crew was arrested by the British authorities a few months later; Anne and Mary resisted fiercely, but were unable to beat back the guards by themselves. Taken to Kingston for trial, the two women were able to stay their execution by revealing they were pregnant. Anne would eventually escape prison with the help of Edward and Ah Tabai, but Mary perished, her health having deteriorated after giving birth. Following a depression brought on by the death of her own child, Anne joined Edward's crew as his quartermaster aboard the Jackdaw. When he left for England in October 1722, Anne opted to stay in the West Indies.

Bartholomew Roberts at Ouidah with his ship and captured merchantmen in the background.

Bartholomew Roberts (1682–1722), born John Roberts, was a Welsh pirate and Sage, who raided ships in the Caribbean and on the West African coast between 1719 and 1722. He is regarded by many historians to be the most successful pirate of all time, judging by the number of ships taken and the amount of goods stolen. For his actions, he earned the moniker Black Bart, although the name was never used in his lifetime. His death is often considered to be when the Golden Age of Piracy truly ended.

Benjamin Hornigold (1680s –1719) was an English pirate from 1715 to 1718, and a member of the Templar Order. He was most famous for mentoring Edward Thatch and for being one of the founders of the Pirate Republic at Nassau.

Mary Read (c. 1685–1721) was an English pirate and member of the Assassin Order, trained by the Mentor of the Caribbean Assassins, Ah Tabai. She was also one of the founders of the Pirate Republic of Nassau. In order to facilitate her career as a pirate, she posed as James Kidd, an illegitimate son of the late William Kidd.

John "Jack" Rackham (1682–1720), more commonly known as Calico Jack, was a Jamaican-born British pirate who operated in the West Indies during the early 18th century. He was the captain of a schooner called the Royal Phoenix, prior to her destruction in 1718. At some point, Rackham began serving as quartermaster under Charles Vane, though he grew to loathe his fellow pirate's hot temper and violent nature over the years. Supported by his crewmates, Rackham seized command in November 1718, deposing Vane as captain and setting him, along with Edward Kenway, adrift in a damaged sloop. To the frustration of his crew, Rackham possessed limited aspirations as a pirate captain, and soon returned to Nassau to accept the King's pardon. There, he entered into a relationship with barmaid Anne Bonny, who convinced him to return to his previous life in August 1720. Together with Mary Read, the duo escaped Nassau and plundered and pillaged for a few months, before being arrested by the British authorities. Tried and convicted of piracy, Rackham was executed in Port Royal on 18 November 1720.

Stede Bonnet (c. 1688–1718) was an early 18th century Barbadian pirate of English descent who sailed the Eastern Seaboard of the Thirteen Colonies with his crew. Despite his infamy, he was often nicknamed "The Gentleman Pirate", due in part to the fact that he was a moderately wealthy landowner prior to turning to piracy, owning a profitable sugar plantation in Barbados. Despite his contrasting upbringing, Stede became a likeable associate of several well-known pirates based in the Caribbean, including Edward Kenway, Edward Thatch, Benjamin Hornigold and Mary Read.

Shay Patrick Cormac (1731–Unknown) was an Irish-American Assassin-turned-Templar during the Seven Years' War. He turned into a Templar with the desire of wanting justice from the Assassins for the deaths of thousands of innocents in Lisbon, Portugal and is responsible for exterminating the Colonial Brotherhood during the Seven Years' War and the murder of Arno Dorian's father, which would later be one of the causes of making him an Assassin. He appears as the protagonist of Assassin's Creed Rogue. Shay's grandson, "Cudgel" Cormac appeared as a professional Assassin hunter who continued his grandfather's legacy in protecting the Templar cause in the novel Last Descendants. He is voiced by actor Steven Piovesan.

Juhani Otso Berg is a member of the Inner Sanctum of the Templar Order. He first appeared as a faceless playable character in Assassin's Creed: Brotherhood's and Assassin's Creed: Revelations' multiplayer modes before being fully realized and seen in Assassin's Creed Rogue. A former member of the Finnish Special Forces-turned-mercenary, Berg was approached by Warren Vidic in 2010 to join Abstergo, who in return will provide cystic fibrosis treatment for his daughter. By 2012, Berg had risen to the rank of Master Templar, and became the leader of Abstergo's elite commando unit, 'Sigma Team'. Berg captured William Miles in Cairo during the events of Assassin's Creed III. He also oversaw the genetic research of Shay Cormac's memories during Assassin's Creed Rogue alongside Templar colleagues Violet de Costa and Melanie Lemay before aiding the Abstergo search for the Shroud of Eden in 2015 during Assassin's Creed Syndicate. During his work for Abstergo he crossed paths with and fought with Shaun Hastings and Rebecca Crane, as well as Russian Master Assassin Galina Voronia.

Violet de Costa is a member of the Inner Sanctum of the Templar Order and Sigma Team's historical research specialist. She oversees the genetic research of Shay Cormac's memories during Assassin's Creed Rogue, guiding the researcher and inviting them to join the Templar Order at the games conclusion. She later participated in the search for the Shroud of Eden in 2015, accompanying Otso Berg and Isabelle Ardant and fighting off Shaun Hastings, Rebecca Crane and Galina Voronia who were also chasing the shroud. Distracted by Otso and the rest of Sigma Team, they were unable to stop Violet escaping with the Shroud. Violet is later revealed to be a member of the Instruments of the First Will, a human cult devoted to Juno in the 21st century.

James Cook (1728–1779), was a Captain of the Royal Navy and master of the HMS Pembroke. During the Seven Years' War, he assisted the Templars and the British during their campaigns against the French and the Assassins, despite not knowing the true existence of the two orders due to his strict honesty which the Templars decided not to induct him or even tell him about their existence. He later assisted Shay Cormac and Haytham Kenway in locating the Precursor Temple in an effort to stop the Assassins. After Shay killed de la Verèndrye, the former gave the latter's maps in different locations which would eventually led Cook in discovering new lands for the British Empire, most notably Australia.

Lawrence Washington. Painted by Gustavus Hesselius, an itinerant painter working out of Annapolis, Maryland.

Lawrence Washington (1718-1752) was a sailor of the Royal Navy and high-ranking member of the Templar Order as well as being the older brother of George Washington. After serving the Royal Navy during the Battle of Cartagena de Indias, he was ordered along with George Monro by British Grand Master Reginald Birch in establishing Templar presence at the British Colonies and was promoted to Master Templar for his actions. When his brother, George, contracted smallpox, he sailed with him to Barbados in finding a cure as well as finding a First Civilization Temple where an artifact may be found. At the same time, the Assassin Vendredi disturbed the artifact, setting off the 1751 Port-au-Prince earthquake and Washington killed him while trying to escape. While returning to the colonies, he built Mount Vernon in memory of his officer, Edward Vernon, and contracted tuberculosis. Just before his death during a garden party, he told his Templar associates Samuel Smith, James Wardrop and Jack Weeks, to keep George out of the Templar cause as he had nothing to do with the order. Just as he was talking to his guests, he was killed by the Assassin, Shay Cormac, who was ashamed of murdering him since he was already dying which would later be one of the causes of his eventual defection to the Templars and the destruction of the Colonial Assassins.

Louis-Joseph Gaultier de La Vérendrye (1717–1761), or simply Chevalier de la Vérendrye, was a French-Canadian explorer and trader, and a member of the Assassin Brotherhood. Before the Seven Years' War, he could be considered as Shay Cormac's rival and the two had an instant dislike to each other when Cormac was introduced into the Assassins and was the best sailor of the Colonial Assassins. He assisted Cormac and his friend, Liam O'Brien in freeing smugglers who were assets of the Brotherhood, though he and Cormac engaged in a fistfight for being late. When Shay stole the Manuscript and was about to kill himself for the disaster at Lisbon, de La Vérendrye shot him at the back, presuming that he killed him, only to be rescued by the Templars. Later, he chose to become a decoy in order to prevent Shay and the Templars from stopping Achilles and Liam from reaching the Arctic Temple. Though he and his fleet ambushed the Templars in a snowstorm, he met his death at the hands of the now-Templar Shay by throwing him under the bottom of the Atlantic Ocean.

George Monro (1700–1757), was a Lieutenant-Colonel of the British Army and a high-ranking member of the Templar Order. He rescued Shay Cormac after he betrayed the Assassins and asked for his help in purging Assassin-allied gangs who terrorized New York. He assisted Cormac in various activities until the latter rescued him and his troops from an ambush by French-allied Indians led by the Assassin Kessegowaase, one of Shay's former tutors, at the aftermath of the Siege of Fort William Henry. Though Shay killed Kessegowaase at Albany, Monro himself was killed by Shay's former friend, Liam, which Monro gave his Templar ring to Shay, motivating him to become a Templar.

Arno Victor Dorian (1768– unknown) was a French-Austrian Assassin during the French Revolution, his mother Maria left him and his father when he was a young child then a few years later his father was killed by Shay Patrick Cormac in Assassin's Creed Rogue. After his biological father's death, he was adopted by François de la Serre, Grand-Master of the Templar Order, and falls in love with de la Serre's daughter, Élise. Arno appears in Assassin's Creed Unity and makes a cameo appearance in 2016's Assassin's Creed movie, where it is discovered that he is an ancestor of Callum Lynch. He is played by Dan Jeannotte.

Elisé de La Serre (1768–1794) was a Templar during the French Revolution and companion and lover of Arno Dorian. Elisé met Arno the day Arno's father was killed. Her father, Grand Master de La Serre, took Arno in and raised him as his ward, allowing Arno and Elisé to grow up together. After her father's death she partially blames Arno as he had not delivered the anonymous warning of the assassination, parting ways on bitter terms. After Arno became an Assassin he found out that the Templar who ordered Elisé's father's murder wanted her dead too. From that point on Arno tried protecting Elisé the moment they met again. He later continued helping her find Germain, the Templar Sage who wanted to rebuild the Templar Order. During the final confrontation between Arno, Elisé and Germain, Elisé was killed, devastating Arno and leaving a big impact on him for the rest of his life.

François de la Serre (1733–1789) was the Grand Master of the Parisian Rite of the Templar Order during the late 18th century. He was the father of Élise de la Serre and adoptive father of Arno Dorian. A supporter of the absolutist monarchy of the Ancien Régime, he expelled his lieutenant, François-Thomas Germain, from the Order for his radical ideas of unseating the aristocracy from power and instead giving it to the rising middle class. Germain came to see de la Serre as a complacent Grand Master who had forgotten the Templars' true goals, and had him killed in 1789 as part of a coup within the Order.

Pierre Bellec (Voiced by Anthony Lemke) (c. 1740–1791) was a French-Canadian Master Assassin, and a former colonial soldier, veteran of the Seven Years' War, who was active during the French Revolution. At some point before the French Revolution, Bellec took a seat on the Assassin Council of the French Brotherhood. Arno Victor Dorian was his student.

Victor and his brother Hugo were two blacksmiths living in Versailles during the late 18th century. The names of Victor and Hugo are a reference to the French poet and novelist Victor Hugo. Hugo mentioned to Arno the reason for his and Victor's imprisonment was stealing a loaf of bread. This is a reference to the main character of Victor Hugo's novel Les Misérables, Jean Valjean, who was imprisoned for 19 years for stealing a loaf of bread

Le Roi des Thunes or The King of Beggars was the self-claimed "king" of the Cour des Miracles district. He forced the beggars of the district to pay him tributes, and to do so they had to pretend to be sick so the rich people would pity them. He tried to join the templars under the command of Francois de la Serre, but De la Serre rejected him and le Roi vowed to kill him. He was approached by Charles Gabriel Sivert, and joined the radical faction of the Templar order led by François Germain. He was the one to murder De la Serre during the 1789 coup, and was assassinated by Arno Dorian two years later.

Aloys la Touche was the right-hand man and enforcer of Le Roi des Thunes, tasked with collecting the tributes from the beggars and punishing the ones who couldn't pay. He was also a templar under the command of François-Thomas Germain, and after le Roi des Thunes was assassinated by Arno Dorian, la Touche was tasked by the templar Maximilien de Robespierre with leading his Reign of Terror in Versailles. He was assassinated by Arno during an execution mass in 1793.

Marie Lévesque was a wealthy noble and one of Paris' main grain merchants. She was a templar and a supporter of Germain, aiding him in the coup against De la Serre. After Germain took over, she was tasked by him to make the people starve, to make the revolution more violent and brutal. Her plans were discovered by Arno Dorian, who assassinated her during a lavish party in 1792.

Frédéric Rouille was a captain of the Paris military and a templar supporter of Germain. He was a leading figure in the Tenth of August and in the September Massacres, where he was assassinate by Arno Dorian.

Jean Lessard (?–1794) was a Sans-culottes leader in Paris who was rejected by Marie Tussaud, and held disgust for her after this. During the revolution, Lessard was bloodthirsty and hungry for revenge against Tussaud, and sent his men to slay her. Tussaud escaped however, and sent Arno Dorian to eliminate Lessard, and the Assassin successfully carried out the task.

Denis Molinier was a French alchemist and member of the French Templars, tasked with finding Nicolas Flamel's laboratory. Arno Dorian collaborated to find the lab before Molinier, and stole one of the mechanisms which opened Flamel's lab from Molinier. It is unknown what happened to him afterwards, either slain in the Revolution, killed by his superiors for his failure or survived safely. He is based on the real life Denis Molinier, who lived from 1711–1777.

Donatien Alphonse François, Marquis de Sade (Voice by Alex Ivanovici) (2 June 1740–2 December 1814), commonly known as the Marquis de Sade, was a French aristocrat, revolutionary politician, philosopher and author famous for his libertine sexuality. He also became the new Roi des Thunes, after the former bearer of the title was assassinated by Arno Dorian in 1791. He had been held prisoner in the Bastille for several years, but was transferred to another prison shortly before angry Parisians stormed the stronghold. Three years later, as Arno and Élise de la Serre hunted Louis-Michel le Peletier, the pair enlisted de Sade's knowledge of the Parisian politics to track le Peletier's whereabouts.

Francois-Thomas Germain (voiced by Julian Casey) (1726–1794) was a French silversmith, Sage and Grand Master of the Parisian Rite of the Templar Order during the French Revolution. He appears in Assassin's Creed Unity. After experiencing visions of the First Civilization and reading the Codex Pater Intellectus, he took it upon himself to reform the Order, which he believed had grown corrupt after aligning itself with the aristocracy for centuries. Inspired by Grand Master Jacques de Molay, Germain sought to carry out the "Great Work" and create a capitalist society in which the Templars could more easily control the populace. Grand Master François de la Serre regarded his ideas as being too radical, and exiled him from the Order. Germain eventually began turning de la Serre's advisors to his cause, forming a radical faction within the Templar Order. In 1789, he had de la Serre assassinated in the Palace of Versailles, leaving most of the Order under his control. As the French Revolution broke out, Germain and his followers exploited discontent with the monarchy and sought to create as much chaos as possible. In this way, the aristocracy and clergy would be crushed and the populace would fear the idea of rising against the establishment again. The Templars hoarded food and framed the royal family, eventually leading to the execution of King Louis XVI in 1793. Under the Templar Maximilien de Robespierre, the Reign of Terror began, bringing the revolution to a violent and chaotic climax. Along the way, Élise de la Serre, the daughter of the deceased Grand Master, opposed Germain. She and her lover, the Assassin Arno Dorian, eventually eliminated Germain's followers and tracked him down to the Temple in 1794. After the Grand Master killed Élise with a Sword of Eden, he himself was killed by Arno. In his final moments, Germain triggered a vision, telling the Assassin that his goals of reforming society and the Templar Order had already succeeded, even if he would not live to see the changes

Honore Gabriel Riqueti, comte de Mirabeau (voiced by Harry Standjofski) (1749–1791) better known as simply Mirabeau, was a French statesman, author, the Mentor of the Assassin Council of the French Brotherhood and a leader of the French Revolution during its early stages. A gifted orator, he criticized France's arbitrary justice system and favored a constitutional monarchy built on the model of Great Britain, being a voice of moderation in the National Constituent Assembly. As Mentor of the Council and Brotherhood, Mirabeau sought to establish peace with the Parisian Rite of the Templar Order and its Grand Master, François de la Serre and inducted Arno Dorian into the Brotherhood. After a coup within the Templar Order, he was forced to give up on the truce, until the Grand Master's daughter, Élise de la Serre, offered to work with the Assassins in 1791. Unlike most of the Council, he was eager to accept this offer. Regarding Mirabeau as a traitor to the Brotherhood, the Assassin Pierre Bellec poisoned him. After his death, it was discovered that Mirabeau had served King Louis XVI as an advisor in exchange for having his debts paid off. Public opinion turned against him, leading to his remains being moved away from the Panthéon. History views him as a complex man who is not easily understood. Historians disagree on whether he was a great leader who might have prevented the Reign of Terror had he lived longer, a demagogue lacking values or a traitor serving enemies of the revolution. Bellec's decision to murder Mirabeau has also led subsequent Assassins to debate whether or not his attempts to reconcile with the Templars conflicted with the ideals of the Brotherhood.

Louis XVI of France (born Louis Auguste de France, also known as Louis Capet) (1754–1793) was King of France and Navarre from 1774 until 1791, after which he was subsequently King of the French from 1791 to 1792, before his deposition and execution during the French Revolution.

Maximilien François Marie Isidore de Robespierre (voiced by Bruce Dunmore) (1758–1794), often known as simply Maximilien Robespierre or Robespierre, was a French lawyer, politician and member of the Templar Order. He was notable for starting the Reign of Terror during the French Revolution, and abolishing slavery in France.

Napoléon Bonaparte (voiced by Brent Skagford) (1769–1821) was a French military and political leader who ruled first as the First Consul of France from 1799 to 1804, then as Emperor from 1804 to 1815. He rose to power amidst the chaos and political turmoil of the French Revolution. He was also an acquaintance to the Assassin Arno Dorian.

Arbaaz Mir (early 1800s –unknown) was a member of the Indian Brotherhood of Assassins during the 19th century, active during the time of the war between the Sikh Empire and the East India Company. Arbaaz is father to Jayadeep Mir (Aka: Henry Green). Arbaaz Mir appears in Assassin's Creed Chronicles: India. He is voiced by Johnny Neal.

Nikolai Andreievich Orelov (late 1800s–1928) was a member of the Russian Brotherhood of Assassins during the late 19th and early 20th century. Nikolai was notably involved in events such as the Borki train disaster and the Tunguska explosion, and spearheaded the hunt for the Imperial Sceptre of the Russian royal family with aid from Nikola Tesla. He later left the Brotherhood out of fear for his and his family's safety and was hunted down by Assassins working for the FBI after he killed one of his fellow Assassins (and a longtime friend) in a fit of paranoid madness after they attempted to convince him to return to the Brotherhood with his son, Innokenti. Orelov appears in Assassin's Creed Chronicles: Russia.

Jacob Frye (1847–unknown) was a British Assassin active in London during the Victorian Era, and the younger twin brother of Evie Frye. Born in a time when the Assassin Order in London was at its weakest in over one hundred years, alongside his sister, he appears in Assassin's Creed Syndicate. He is voiced by Paul Amos.

Evie Frye (1847–unknown) was a British Assassin active in London during the Victorian Era, and the older twin sister to Jacob Frye. Born in a time when the Assassin Order in London was at its weakest in over one hundred years, alongside her brother, she appears in Assassin's Creed Syndicate. She is voiced by Victoria Atkin.

Galina Voronina is a Russian Master Assassin. Like Desmond, she acquired her abilities by reliving her ancestors' memories in the Animus and taking advantage of the Bleeding effect. Forcibly entered into the Animus by her mother, she eventually contacted the Assassin Gavin Banks for help. She was able to escape, but not before the death of her sister and her mother's suicide. Following her prolonged exposure to the Animus, her mental health is in question; Rebecca having admitted to seeing Galina believing she was conversing with her dead sister. A talented Assassin, she takes on Sigma Team almost single-handedly in during Assassin's Creed Syndicate. About to kill Otso Berg, she is forced to cover Shaun and Rebecca's escape, after the latter is shot.

Lydia Frye is the grand-daughter of Jacob Frye, and a British Assassin operating in London during World War I. She crossed paths with Winston Churchill and assassinated Templar agents attempting to prolong the war, one of which was a Sage. She appears within the Helix missions in Assassin's Creed: Syndicate.

Henry Green was the India-born leader of the London Assassins during the late 1860s. Born Jayadeep Mir, the son of Arbaaz Mir, he crossed paths with the Frye Twins, Evie and Jacob during their liberation of London in 1868, aiding them in their final fight against Crawford Starrick and eventually marrying Evie. As a child in India, he was trained by the twins' father, Ethan Frye.

Crawford Starrick (1827–1868) was the owner of Starrick Industries, and the Grand Master of the British Rite of the Templar Order by 1868. He appears in Assassin's Creed Syndicate. As an arrogant individual, he showed great disdain to those who tormented him and eventually saw himself as a ruler among servants. Using his charm, he managed to bend people to his will. After all the members of his inner circle were killed by the Frye twins, Starrick went himself to the location of the Shroud of Eden, where he was killed by the twins.

Lucy Thorne (1837–1868) was a Templar and Grand Master Starrick's second-in-command. Lucy was also the Order's expert on Pieces of Eden, and was tasked with finding the legendary Shroud of Eden. After fellow templar Pearl Attaway was killed by Jacob, Lucy relentlessly chased the twins in search of revenge, only to be assassinated by Evie.

Maxwell Roth (18??–1868) was the mastermind behind London's criminal underworld and one of Starrick's lieutenants. Although he wasn't a Templar himself, he trained several of Starrick's small-time gang leaders in return for a massive amount of money. After some of Starrick's lieutenants were killed, Roth proposed a partnership with Jacob's gang, betraying Starrick and helping Jacob's gangsters in freeing the city. The partnership ended shortly after due to Roth's psychopathic behavior, and after he intentionally burned the Alhambra Music Hall during his final act, Jacob stabbed him with his blade. In his final moments, Roth told Jacob that he had carried out the atrocities simply because he wanted and chose to do so.

Pearl Attaway was the owner of Attaway transport and, like her cousin, Grand Master Crawford Starrick, a member of the British Templars. Attaway was business rivals with Malcolm Millner, another Templar. Starrick initially choose Millner as the man controlling the bus transport, due to tensions with Attaway, but after Attaway collaborated with Jacob Frye, who didn't know that she was a Templar, Starrick gave the reins to Attaway and they both orchestrated Millner's elimination. Jacob subsequently found out Attaway's true colors, and killed her in her train station.

Rupert Ferris was the owner of Ferris Ironworks, a large steel-mill in the city of Croydon near London, effectively controlling the small city with his wealth. He was also one of Starrick's lieutenants. Ferris was killed by Jacob Frye in his factory.

Philip Twopenny was the governor of the Bank of England and a secret member of the Templar Order. He orchestrated various robberies within the bank to fund the Templars, and during one of these raids, Twopenny was assassinated by Jacob Frye with the help of inspector Abberline.

Darwin's detailed journal of his voyage aboard HMS Beagle made him famous in scientific circles.[16] Upon his return, his father helped Darwin invest so that he could function as a self-funded gentleman scientist.[16] I'd love to have that on by business cards.[16] Darwin's many successes came at a cost, though, as the man was plagued by various illnesses caused by overwork.[16]

Darwin's On the Origin of Species was published in 1859.[16] By the 1870s, the scientific community and much of the general public had accepted evolution as a fact.[16] (By the 2000s, in - ahem - certain countries I absolutely refuse to name, much of the general public appears to have taken a big step backwards! I'm talking about America).[16]

Darwin was in international celebrity, who is remembered for providing a unified theory of the life sciences that explains the wide diversity of life on this planet.[16] He was honourwed with a burial in Westminster Abbey.[16]

Charles Dickens (1812–1870) was an English writer and social critic. He created some of the world's best-known fictional characters and is regarded as the greatest novelist of the Victorian era. As Darwin, he also met Jacob and Evie Frye, and asked their help in Assassin's Creed Syndicate.

Karl Marx (1818–1883) was a German philosopher, economist, communist, sociologist, journalist and revolutionary socialist. He asked Jacob and Evie Frye's help to expand his unionistic ideas to the people of London.

Sir Arthur Conan Doyle (22 May 1859–7 July 1930), known as Artie in his younger years, was a Scottish writer and physician, famous for his fictional stories about the detective Sherlock Holmes. In 1868, while still a young boy, his love for detective stories led him to team up with the penny dreadful writer Henry Raymond and the Assassins Jacob and Evie Frye to solve murders.

Alexander Graham Bell (1847–1922) was a Scottish-born scientist, inventor, engineer and innovator who was credited with inventing the first practical telephone. He was on friendly terms with Henry Green, who nicknamed him, "Aleck".

Queen Victoria (1819–1901), born Alexandrina Victoria of Kent, was the Queen of the United Kingdom of Great Britain and Ireland from 1837, and Empress of India from 1876, until her death. Her reign of sixty-three years became known as the Victorian era; marked by the Industrial Revolution and Pax Britannica, it was a period of great expansion for the British Empire, and was consequently a period of significant cultural, political, scientific, and military change across the globe.

Duleep Singh (1838–1893), also known as Dalip Singh or the Black Prince of Perthshire, was the last Maharaja of the Sikh Empire and the youngest son of Ranjit Singh, ruling from 1843 to 1846. He was also an associate of, and great-uncle to, the Assassin Henry Green. Exiled to Britain when he was fifteen, Duleep befriended Queen Victoria, who would go on to become the godmother to several of his children. After his mother died in 1863, he began to lobby for India's independence from the British Empire.

Jack (unknown–1888), formerly known as Jack the Lad during his youth and widely feared as Jack the Ripper, was a member of the British Brotherhood of Assassins, active in the Whitechapel district of London up until 1888. Jack became infamous in 1888 following a series of gruesome murders of young women, which were fellow Assassins trying to stop his madness; these murders, in conjunction with Jack's control over London's criminal enterprise, threatened the very existence of the Assassins in London. He is killed by Evie Frye.

David Brewster, a scientist working on a Piece of Eden at the behest of the Templar Order. He is assassinated by Evie Frye and his experiments on the Piece of Eden grow unstable, resulting in an explosion that buries his underground laboratory.

John Elliotson, a physician working in Lambeth Asylum and an associate of Crawford Starrick. Elliotson created "Starrick's Soothing Syrup", a highly-addictive tonic made from opium and hallucinogens used to keep the working classes under control. He is assassinated by Jacob Frye, but his death comes with consequences; the borough of Lambeth is flooded with counterfeit medicinal and sub-standard medical care.

James Brudenell, 7th Earl of Cardigan, a member of the House of Lords who opposes Benjamin Disraeli and the Corrupt Practices Act as he tries to keep political power in the hands of the nobility. Although he is a part of Starrick's conspiracy to control London, it is implied that he is an unwitting puppet, easily manipulated but otherwise incompetent because of his role in the Siege of Balaclava and the Crimean War.

Callum Lynch (portrayed by Michael Fassbender)[17][18][19] (b. 1979) is a descendant of Aguilar de Nerha, a Spanish Assassin. After being rescued from his own execution by Abstergo, he is placed in the Animus to relive the memories of his ancestor Aguilar de Nerha and find the Apple of Eden. After reconciling with his father, who murdered his mother to keep her out of Templar hands, he joins the Assassin Order, retrieves the Apple, and kills Alan Rikkin.

Aguilar de Nerha (portrayed by Michael Fassbender) was a Spanish Assassin and ancestor to Callum Lynch. During the Spanish Inquisition, he attempted to protect the son of Sultan Muhammad XII of Granada, an ally of the Assassins who possessed an Apple of Eden. He eventually managed to escape with the Apple and passed it on to Christopher Columbus for protection.

Alan Rikkin (portrayed by Jeremy Irons) (unknown–2016) was the CEO of Abstergo. He had sent several e-mails to Warren Vidic, one of which that details several Pieces of Eden that they believe to be nothing more than mythical tales, including the Holy Grail. Rikkin is possibly one of the three men standing in the conference room right after Desmond Miles finished the Altaïr project. He appears in the Assassin's Creed film, where he seeks the Apple of Eden through the memories of Aguilar de Nerha. He eventually discovers the Apple in the tomb of Christopher Columbus and attempts to perform a ritual to remove human free will, but he is assassinated by de Nerha's descendant Callum Lynch, who recovers the Apple.

Sofia Rikkin (portrayed by Marion Cotillard)[20] (1980–unknown) is a scientist and the head of the Abstergo Foundation Rehabilitation Center in Madrid. She is also the daughter of Alan Rikkin, the former CEO of Abstergo Industries and part of the Templar Order's Inner Sanctum, with whom she had a difficult relationship. In October 2016, Sofia came into contact with Callum Lynch, a criminal whose Assassin ancestry was of interest to the company. During their time together the pair formed a connection but when Lynch's escape from the Foundation led to the death of Alan Rikkin at his hand, Sofia dedicated herself to chasing down her father's killer.

Maria (portrayed by Ariane Labed) (unknown–1492) was a member of the Spanish Brotherhood of Assassins during the 15th century, as well as a close ally of Master Assassin Aguilar de Nerha. In 1492, she and her fellow Assassins sought to prevent Prince Ahmed of Granada from being captured by the Templars, fearing that his father, Sultan Muhammad XII of Granada, would relinquish the Apple of Eden in his possession in exchange for his son's safety.

Alicia Vikander was originally considered for the role of Maria, but scheduling conflicts with Jason Bourne eventually caused the actress to drop out, with Ariane Labed being cast instead.[21]

Tomás de Torquemada (portrayed by Javier Gutiérrez) (1420–1498) was a Spanish Dominican friar, the first Inquisitor General of Spain, confessor to Isabella I of Castile, and a Master Templar of the Spanish Rite of the Templar Order. Under influence of Rodrigo Borgia, Grand Master of the Italian Templars and Papal candidate, Torquemada opposed the Spanish Assassin Brotherhood, persecuting them as part of the Spanish Inquisition.

Bayek of Siwa (portrayed by Abubakar Salim) was Medjay and Assassin operating in the Ptolemaic Kingdom of ancient Egypt, around 49 BCE, who appears in Assassin's Creed Origins. Bayek possessed a symbiotic relationship with the eagle, Senu, which allowed him to see the world through Senu's eyes - a more literal predecessor to the Eagle Vision of later Assassins. He is also regarded as the founder of the organised Assassin Order.

Cleopatra VII Thea Philopator (69 BC–12 August 30 BC), commonly known as Cleopatra was the last Egyptianpharaoh and a member of the Ptolemaic dynasty. She initially shared power with her father, and later, her brothers, whom she married. With the help of the Templars, she became the sole ruler of Egypt. This also helped her become an ally of Julius Caesar. After Caesar's death, she allied herself with Mark Antony, whom she had a relationship with. In 30 BC, Antony committed suicide at the Battle of Actium. Soon afterwards, Cleopatra was poisoned by the Assassin Amunet with an asp.

Amunet, better known as Aya of Alexandria, wife of Hidden Ones founder Bayek of Siwa, was a female member of the Egyptian Order of Assassins during the Hellenistic period of the EgyptianPtolemaic Dynasty. On 12 August 30 BCE, Amunet infiltrated Cleopatra VII's palace. There, she killed the last pharaoh of Ancient Egypt using a venomous asp. By the Renaissance, Amunet was respected as a great Assassin, and a statue of her was placed in the Assassin Sanctuary of Monteriggioni. Around this time, her sarcophagus was moved to the Basilica di San Marco in Venice, Italy.

Gaius Julius Caesar (13 July 100 BC - 15 March 44 BC), commonly known as Julius Caesar was a prominent general in the Roman army and a notable politician. He played a key role in transforming the flagging Roman Republic to the seemingly unstoppable Roman Empire. He was killed due to his Templar affiliations. Caesar gained his fame on military campaigns in Gaul, where he conquered what is present-day France, Germany, and Belgium. In 50 BC, Caesar was called back to Rome, and he feared he might be prosecuted for insubordination and treason. Caesar brought one of his legions with him and started a civil war. During this conflict, Caesar was made Dictator of Rome. Roman Senators feared Caesar's in increasing military power, and also hated him due to the fact he was supported by the Templars. On March 15, 44 BC, also known as the Ides of March, the Senators planned their strike. A group of Roman Senators, who were also Assassins, led by Brutus and Cassius, stabbed Caesar twenty-three times in the Roman Senate. The Senators thought that by ending Caesar's life, they had helped save Rome, but they actually put Rome in chaos. For the next thirteen years, civil war raged through the Roman Republic. The outcome was the birth of the Roman Empire, and the fall of the Egyptian Pharaohs.

Marcus Junius Brutus the Younger (early BC June 85 – late BC October 42), more commonly known simply as Brutus, was a politician of the late Roman Republic, a member of the Roman Republic and the Order of Assassins. Most notably, Brutus held a leading role in the assassination conspiracy against the consul Gaius Julius Caesar in 44 BCE. He was also the first known human to discover the Colosseum Vault.

Qulan Gal was a member of the Mongolian Assassin Brotherhood, operating mainly in Mongolia. He was a renowned bowman, a skill which he was later hailed for by the Order. During the early 13th century, the conqueror Genghis Khan caused turmoil in Mongolia, quickly overtaking land, suspected with the usage of a Piece of Eden. As a result of Genghis Khan's tyranny, Qulan Gal swore to stand against him. Qulan Gal was one of the Order's most renowned Assassins due to his vital role in the assassination of Genghis Khan alongside Darim Ibn-La'Ahad, the eldest son of Altaïr Ibn-La'Ahad. At some point during 1217 and 1227, Qulan Gal came into contact with the Mentor of the Levantine Assassins, Altaïr Ibn-La'Ahad, his wife Maria Thorpe and his son Darim Ibn-La'Ahad. In 1227, the four of them devised a plan to assassinate Genghis Khan in his camp near Xing-ging in Xu Province. While Qulan Gal and Altaïr went into the camp together, Darim covered them from a distance with his bow. However, Altaïr allowed himself to be seen and was attacked by a Mongol soldier, though Qulan Gal killed the Mongol before he could raise the alarm. Qulan Gal safely escorted Altaïr out of the camp and proceeded to make another plan with Darim. Qulan Gal and Darim then devised a plan to flush Khan out of his tent, and the latter did so after realizing the danger. Qulan Gal acted quickly and shot Khan's horse, causing him to fall off, killing him on impact. Darim shot the conqueror with his crossbow for assurance. Qulan Gal's role in the assassination of Genghis Khan, allowing the Assassin Order to spread its influence, earned Qulan Gal great praise, and a statue of Qulan Gal was built in the sanctuary underneath the Villa Auditore in Monteriggioni, Italy. By the time of the Renaissance, Qulan Gal's sarcophagus was moved to the Rocca di Ravaldino in Forlì, Italy.

Darius was a male member of the Persian Order of Assassins during the Achaemenid Persian Dynasty. In the 5th century BCE, the Templars supported the reigns and conquests of the Achaemenid kings Darius I and his son Xerxes I. Using a newly created weapon named the hidden blade, which would later become the Assassins' iconic signature weapon, Darius personally assassinated King Xerxes I. By the Renaissance, Darius' remains were transferred to the Santa Maria Novella in Florence, Italy, where his body lay with an Assassin Seal needed to get access to the armor of Altaïr Ibn-La'Ahad hidden inside the sanctuary in Monteriggioni. In 1478, his tomb was explored and sarcophagus was opened by Ezio Auditore da Firenze, who took the Seal and later obtained the armor of Altaïr Ibn-La'Ahad.

Iltani was a female member of the Babylonian Order of Assassins during the Argead dynasty. During her lifetime, Alexander III of Macedon conquered most of South-West Asia by using a Piece of Eden. Some time prior to 10 or 11 June 323 BC, Iltani had poisoned Alexander the Great, who died as a result on 10 or 11 June 323 BC. By the time of the Renaissance, Iltani's sarcophagus was moved to Santa Maria del Fiore in Florence. Fellow Assassin Ezio Auditore da Firenze explored her tomb and took the seal from her sarcophagus, which helped him to acquire the armor of Altaïr Ibn-La'Ahad. In the year 2000, a statue of Iltani stood in the office of the Assassin Order's Mentor in Dubai.

Wei Yu was a member of the Chinese Order of Assassins during the imperial Qín Dynasty of China, which was ruled by Emperor Qin Shi Huang, a Templar-backed tyrant who oppressed the people of China. In order to protect the people, the Assassins resolved that the land had to be freed from the Emperor's oppressive control. On September 10, 210 BC, during one of the Emperor's Eastern China tours, Wei Yu struck the Emperor with his spear, killing him. By the time of the Renaissance, Wéi Yù's sarcophagus was moved to San Gimignano, Italy, on the top floor of the Torre Grossa. Fellow Assassin Ezio Auditore da Firenze would later explore his tomb and take the hidden seal from inside the sarcophagus, which would help him to obtain Altaïr Ibn-La'Ahad's armor.

Leonius was a male member of the Roman Empire Order of Assassins during Italy's Julio-Claudian dynasty. The Assassins considered the Emperor, Gaius Julius Caesar Augustus Germanicus's, commonly referred to as Caligula, a tyrant and the land had to be freed of him. On 24 January, 41 A.D. Leonius stabbed Caligula, killing him. Between the time of his death and the Renaissance, Leonius' sarcophagus was moved to Venice, Italy, beneath Santa Maria della Visitazione, Venice.

Aquilus (unknown-259) was a Gallo-Roman member of the Assassin Brotherhood in Lugdunum, France, and a distant ancestor to Desmond Miles. He appears in the French comic, Assassin's Creed. In 259, Aquilus was tasked with the assassination of several high-ranked individuals within the Roman Empire. Having failed to assassinate his third target, General Gracchus, Aquilus met up with his cousin Accipiter – an Aleman and a fellow Assassin – from whom he obtained an ancient artifact known as the "Ankh", formerly held by the Egyptian goddess Isis. After his father was murdered and the Ankh was stolen by the Roman Senator Caïus Fulvus Vultur, Aquilus traveled from Lugdunum to the Empire's capital of Rome, seeking vengeance. Successful in his objective, he returned home with the artifact, discovering more of its abilities. Eventually, Aquilus was executed by Roman soldiers.

Minerva (voiced by Margaret Easley), also known as both Merva and Mera, is a member of the First Civilization introduced in Assassin's Creed II. She is a member of the First Civilization's ruling body, the Capitoline Triad, along with Tinia and Juno. Minerva appears to Ezio, and by extension, Desmond, in the Vatican Vault. Knowing that Desmond will eventually relive Ezio's memories, Minerva essentially uses him as a conduit. She explains that her kind are not gods, but a more advanced civilization whose technology was mistaken for magic. They engineered humans as slaves, but they rebelled. Minerva describes how war with humanity, combined with a devastating cataclysmic event, led to the downfall of their civilization and the extinction of their race. Correctly predicting that a second cataclysmic event would eventually occur, the Triad hid away the tools humanity would need to survive in several vaults throughout the world, and devised a system by which they could communicate across time before they eventually died. She instructs Desmond to find the vaults, then disappears, leaving a confused Ezio in her wake. In Assassin's Creed III, she projects herself through the past one last time and finds Desmond in the Vault. Once there, she warns him about Juno's power-hungry ambitions, hoping that he will decide not to save the world and keep Juno imprisoned. Juno turns the tables and forces Minerva to reveal Desmond's destiny and how his legacy will be manipulated if he allows the cataclysm to happen. During the conversation, Minerva displays a respect of human free will, not unlike the Assassins themselves.

Juno (voiced by Nadia Verrucci), alternately known as Uni, is a member of the Capitoline Triad. She is introduced in Assassin's Creed: Brotherhood, reiterating Minerva's warning to Desmond, but is more hostile and contemptuous of humanity (displaying a mindset similar to that of the Templars) than the other two members of the Triad. After Desmond recovers an Apple of Eden hidden by Ezio in the Roman Colosseum, Juno takes control of Desmond, forcing him to kill Lucy, and instructs him to find "the one who would accompany you through the gate." In Assassins Creed III, after the player kills Warren Vidic and Daniel Cross, she takes the place of the modern-day main antagonist. It is revealed at the end of Assassin's Creed III that Juno sought to conquer the rejuvenating world. When she tried to use Minerva's technology for her own ends, she was found and imprisoned in the Vault, with the other two members of the Triad hoping that she would fade away in time for the devices there to be activated safely. But she endured and confronted Desmond along with Minerva when the time came to use the device. Juno spurs Minerva into showing the inevitable future if Desmond does not activate the device: humanity will start out well, but will simply sink back into their old ways and repeat the cycle all over again. Desmond decides to use the device, preventing the apocalypse, even though this would release Juno and cost him his life. Juno is released, and thanks Desmond's body before leaving her prison. She reappears later in Assassin's Creed Syndicate, where she has created a rogue faction of Templars who are loyal to her and are trying to find a way to restore her physical form, even if it means directly undermining their own organization.

Jupiter (voiced by Peter Renaday), also known as Tinia, was a member of the Capitoline Triad. He appears to Desmond in Assassin's Creed: Revelations, during the Nexus of Time. He elaborates more on the creation of the vaults, and shows the location of the central vault that contains all their accumulated knowledge. Although the humans tend to use his Roman name, Minerva consistently refers to him by his Etruscan name, Tinia.

Aita was Juno's husband, who volunteered to be a test subject for one of the Capitoline Triad's attempts to preserve the First Civilization. The experiment that he took part in is left vague, but Juno suggests that it preserved his body at the cost of his mind. Juno euthanized him when his mind "became brittle", and it may be this event that caused her to turn on Minerva and Jupiter. Via his reincarnations, Bartholomew Roberts and John, he appears in Black Flag. Roberts is killed by Edward while John commits suicide by forcing Abstergo security to shoot him, his body is then taken by Abstergo to use in the mysterious Phoenix Project. Aita is subsequently reborn as the Sage in the form of Jacques de Molay and François-Thomas Germain in Assassin's Creed Unity and an unnamed German spymaster working in London during the events of Assassin's Creed Syndicate. As Germain, he attempts to purge the Templar Order of complacency and restore its original purpose; as the German spymaster, his objectives are unknown, but as he is assassinated by Lydia Frye relatively quickly, it is noted that he was a particularly ineffective Sage. In the role of the Sage, Aita is closely tied to the Instruments of the First Will, a cult worshipping Juno that believes humanity should be subservient to the Precursor race. Aita is the Etruscan name for Hades and Pluto.

^Elizabeth Rawson, "Caesar: Civil War and Dictatorship," in The Cambridge Ancient History: The Last Age of the Roman Republic 146–43 BC (Cambridge University Press, 1994), vol. 9, p. 465.

^Plutarch. "Life of Caesar". University of Chicago. p. 595. ...at this juncture Decimus Brutus, surnamed Albinus, who was so trusted by Caesar that he was entered in his will as his second heir, but was partner in the conspiracy of the other Brutus and Cassius, fearing that if Caesar should elude that day, their undertaking would become known, ridiculed the seers and chided Caesar for laying himself open to malicious charges on the part of the senators...

^Suetonius (121). "De Vita Caesarum" [The Twelve Casesars]. University of Chicago. p. 107. More than sixty joined the conspiracy against [Caesar], led by Gaius Cassius and Marcus and Decimus Brutus.