Qproteome Mitochondria Isolation Kit

The Qproteome Mitochondria Isolation Kit provides fully active mitochondrial preparations of very high purity in a fast, reproducible, and standardized procedure that uses a standard bench-top centrifuge.

The Qproteome Mitochondria Isolation Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

More intact mitochondria per milligram protein.

Mitochondria were isolated using the Qproteome Mitochondria Isolation Kit or another commercially available kit (Supplier S) according to manufacturer’s instructions. Mitochondrial membrane integrity is indicated by formation of fluorescent aggregates following uptake of a carbocyanine dye.

High purity mitochondria with intact mitochondrial membranes.

[A] Mitochondria were prepared from 60 mg of liver tissue using the Qproteome Mitochondria Isolation Kit and subjected to 2D-PAGE. Out of 384 spots picked, 259 proteins were identified by MALDI-MS, 219 (85%) of which were predicted to have a mitochondrial localization. Green circles indicate mitochondrial proteins, red circles indicate impurities, the black circle indicates a protein which is probably located in mitochondria and ER. [B] Electron micrographs of mitochondria isolated using the Qproteome Mitochondria Isolation Kit showing intact inner and outer membranes.

One of the major problems facing researchers working with mitochondria is efficiently separating mitochondrial and cytosolic fractions. Traditional preparation methods are tedious, time-consuming, and require access to an ultracentrifuge. The Qproteome Mitochondria Isolation Kit provides fully active mitochondrial preparations of very high purity in a fast, reproducible, and standardized procedure that uses a standard bench-top centrifuge.

Applications

Mitochondria are the subject of intense study in a number of fields, including apoptosis, proteomics, biomedical research, and drug discovery. The Qproteome Mitochondria Isolation Kit delivers high-purity mitochondria whose proteins retain their native conformation and full biological activity — making them highly suited for apoptosis studies, 2D-PAGE analysis, and other biochemical assays.