For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Shipping Condition

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Preparing Stock Solutions of [Pyr1]-Apelin-13

1 mg

5 mg

10 mg

20 mg

25 mg

1 mM

0.652 mL

3.2599 mL

6.5197 mL

13.0394 mL

16.2993 mL

5 mM

0.1304 mL

0.652 mL

1.3039 mL

2.6079 mL

3.2599 mL

10 mM

0.0652 mL

0.326 mL

0.652 mL

1.3039 mL

1.6299 mL

50 mM

0.013 mL

0.0652 mL

0.1304 mL

0.2608 mL

0.326 mL

100 mM

0.0065 mL

0.0326 mL

0.0652 mL

0.1304 mL

0.163 mL

* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

Ischemic heart disease (IHD) is the main cause of mortality in the world. After myocardial infarction (MI) cardiomyocytes apoptosis and ventricular remodeling have been occurred. Apelin is a peptide that has been shown to exert cardioprotective effects.

[Effects of (Pyr1)apelin-13 on the proliferation and activation of Jarkat T lymphocyte].[Pubmed: 27916097]

Objective To investigate the effect of (Pyr1)apelin-13 on the proliferation and activation of Jurkat T lymphocytes. Methods Jurkat T cells were treated by 0, 10, 50 nmol/L (Pyr1)apelin-13 for 24 hours and cell proliferation ability was detected by CCK-8 assay. The effect of (Pyr1)apelin-13 on the activation of Jurkat T lymphocytes with anti-CD3 and anti-CD28 antibodies was observed and the molecular mechanism was investigated. Quantitative real-time PCR was applied to detect the expressions of CD69, CD25, cytotoxic T lymphocyte antigen-4 (CTLA-4) and programmed death-1 (PD-1) mRNAs. Results The proliferation of Jurkat T cells was not affected by (Pyr1) apelin-13. However, (Pyr1)apelin-13 increased the expressions of CD69 and CD25 and then activated Jurkat T lymphocytes. Furthermore, the expressions of CTLA-4 and PD-1 had no difference between activation group and (Pyr1)apelin-13 group. Conclusion (Pyr1)apelin-13 promotes Jurkat T lymphocyte activation by increasing the expressions of CD69 and CD25.