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Proprietary Technologies Available for Licensing

293-Free™ Transfection Reagent

The 293-Free™ Transfection Reagent features a proprietary polycationic liposomal formulation and is designed expressly for transfection of HEK293 cells grown in suspension culture. It is derived from non-animal sources and is ideal for mammalian protein production. This reagent is sold for research use and requires a license from Merck for any commercial use.

AccepTor™ Vectors

The AccepTor™ Vector Cloning Kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (i.e. KOD XL polymerase and native and recombinant Taq polymerases), which leave single 3'-dA overhangs on the reaction products. The AccepTor Vectors enable direct ligation by virtue of single 3′-dU overhangs that anneal efficiently with 3′-dA extensions on PCR products. The dU residues are converted to dT residues in vivo following transformation. Protected under US Patent 5,856,144.

Beclin Autophagy-Inducing Peptide

Beclin-1 is the mammalian orthologue of yeast Atg6 and plays a central role in autophagy, a process of programmed cell survival, which is increased during periods of cell stress and extinguished during the cell cycle. The Beclin Autophagy-Inducing Peptide is comprised of Beclin-1 residues 269-283 and a heterologous moiety that promotes therapeutic stability or delivery of the compound. The compound may be used to induce autophagy and in assays with Beclin-1 binding partners. Protected under US Patent 8,722,628 and related foreign equivalents.

Histone H3.3 Mutant Antibodies

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone variant H3.3 is typically enriched in active chromatin. Certain non-conservative variants of H3.3 are expressed in cells and tissues afflicted by a number of proliferative disorders. These non-conservative variants are distinct in at least one of two positions when compared to wild-type H3.3 protein. Antibodies to H3.3 variants are protected under US Patent 9,494,591 and related foreign equivalents.

ILK Proteins

Integrin-linked kinase (ILK) is a serine-threonine kinase that is implicated in cell-to-extracellular matrix interaction, growth factor signaling, cell survival, cell migration, invasion, anchorage-independent growth, and angiogenesis. Increased expression of ILK has been associated with the progression of several tumor types. ILK polypeptides are protected under US Patent 7,189,802 and related foreign equivalents.

ITGA 10 Proteins and Antibodies

Integrins are transmembrane proteins involved in cell-to-cell interactions and cell-to-extracellular matrix adhesion. They play important roles in cell migration, development, wound healing, differentiation, and apoptosis. Integrin alpha 10 subunit (ITGA 10), along with integrin beta 1, are thought to be an essential part of a collagen type II receptor expressed mainly in chondrocytes and cartilage tissue. ITGA 10 polypeptides, antibodies, and detection methods are protected under US Patents 7,029,858, 8,048,991, 8,895,253 and related foreign equivalents.

Laminin-2 Proteins

Laminins are a family of large trimeric basement membrane glycoproteins composed of three non-identical chains (alpha, beta, and gamma). Laminin-2 (also known as merosin) consists of alpha-2, beta-1, and gamma-1 chains and is specifically enriched in the basement membranes surrounding skeletal muscle fibers and peripheral nerves. Laminin-2 polypeptides are protected under US Patent 6,632,790.

MAZ51 Compound

MAZ51 is a cell-permeable 3-substituted indolin-2-one compound that acts as a reversible and ATP-competitive VEGF receptor (VEGFR) tyrosine kinase inhibitor. At low concentration, it specifically blocks VEGFR-3 phosphorylation in PAE cells and only partially blocks VEGFR-2 phosphorylation at higher concentrations. This differential blocking effect may be useful for inhibiting certain pathological processes such as tumor metastasis. MAZ51 and methods of using this compound to inhibit VEGFR-3 activity are protected under US Patent 8,324,267 and related foreign equivalents.

Methylarginine Antibodies

Protein methylation is a post-translational modification that plays an important role in several cellular mechanisms, including transcriptional regulation, signal transduction, RNA metabolism, and DNA repair. N-methylation is performed by the PRMT (protein arginine N-methyltransferase) family of enzymes, which introduce methyl groups on arginine residues in proteins, thereby modulating their function. Methylarginine-specific antibodies are protected under US Patent 6,699,673.

MSK1/MSK2 Proteins

Mitogen-and-Stress-Activated Protein Kinase 1 and 2 (MSK1, MSK2) are unique nuclear serine/threonine protein kinases containing two kinase domains within a single polypeptide. These kinases are believed to mediate signal transduction in the inflammatory response and regulate cellular proliferation and differentiation. MSK1 and MSK2 are activated by the MAPK/ERK pathway (mitogen/growth factor signaling) or SAPK2/p38 pathway (stress/oxidation signaling) and activate transcription factors ATF-1 CREB. Protected under US Patent 7,122,360 and related foreign equivalents.

Nus-Tag® Fusion System

The NusA protein is an E. coli protein that was identified by solubility modeling as having a very high probability of being soluble when expressed in E. coli. When fused to heterologous proteins, NusA has been shown to increase the solubility of target proteins in E. coli, thus circumventing the formation of inclusion bodies common to heterologous protein expression in E. coli. Nus-Tag® Vectors have been developed by Merck to allow cloning and high-level expression of the NusA protein fused to a protein of interest. Protected under US Patents 5,989,868 and 6,207,420.

pET Expression System

The pET Expression System allows for target gene cloning and recombinant protein expression in E coli. Target genes are cloned in pET vectors under control of the T7 promoter and gene expression is induced by providing a source of T7 RNA Polymerase in the host cell. Commercial use of the pET System requires a license from Merck.

PopCulture® Reagent

PopCulture Reagent is a buffered mixture of concentrated detergents formulated to extract proteins from E. coli cells directly in their culture medium. Using this method, cell culture, protein extraction and purification can be performed in the original culture tube or multiwell plate. PopCulture perforates the E. coli cell wall without denaturing soluble protein and protects protein from the pH extremes produced in high density culture media. Recombinant proteins can be assayed directly or purified by adding an affinity matrix, washing the matrix-target protein complex to remove culture medium and cellular contaminants and eluting the purified protein from the matrix. Protected under EU Patent 1,432,822.

PS48 Compound

PS48 is an allosteric small molecule that activates PDK1 (3′-phosphoinositide-dependent kinase-1) and is believed to metabolically facilitate a conversion from mitochondrial oxidation to glycolysis during the reprogramming process. PS48 has been shown to enhance reprogramming efficiency in induced pluripotent stem cells (iPS) by about 15-fold. PS48 is protected under US Patent 8,912,186 and related foreign equivalents.

pTriEx™ Expression Vectors

The pTriEx™ Expression Vectors are triple host vectors capable of expressing an inserted protein coding DNA sequence in any of three distinct host systems. The vectors include three promoters, β-actin or CMV (vertebrate), T7lac (bacterial), and p10 (insect), such that the protein coding sequence can be cloned into the vector once and then allow for protein expression in all three types of host cells. The arrangement and selection of the promoters contribute to expression characteristics in all three host systems that are comparable to single host expression vectors. Protected under US Patent 6,589,783.

RS-1 (RAD51-Stimulatory Compound)

RS-1 (RAD51-stimulatory compound 1) is a cell-permeable sulfonamido-benzamide-based allosteric regulator that stimulates the human homologous recombination protein RAD51. RS-1 stimulates binding of hRAD51 to single stranded DNA and enhances recombinogenic activity by stabilizing the active form of hRAD51 filaments without inhibiting hRAD51 ATPase activity. RS-1 has been shown to enhance CRISPR-Cas9 knock-in efficiency in HEK293A cells and to enhance both TALEN and Cas9-mediated knock-in efficiency in rabbits. Methods of using RS-1 to stimulate RAD51 activity are protected under US Patent 8,597,949.

Sulfotyrosine Antibodies

Sulfotyrosine is a common post-translational modification of secretory, plasma membrane, and lysosomal proteins. Sulfotyrosine mediates many receptor-ligand binding interactions, including P-selectin glycoprotein ligand-1 with P-selectin, factor VIII binding to von Willebrand factor, and interactions between thrombin and hirudin. Sulfotyrosine is also involved in the entry of several parasites and viruses. Antibodies to Sulfotyrosine are protected under US Patents 7,589,182 and 7,868,152.

Wnt Inhibitors

The Wnt signaling pathways are a group of signal transduction pathways involved in embryonic development, tissue regeneration, and carcinogenesis. Inhibitors of the Wnt signaling pathway include several IWP (Inhibitor of Wnt Production) and IWR (Inhibitor of Wnt Response) compounds. Wnt inhibitors and methods for their use are protected under US Patents 8,445,491 and 9,045,416 and related foreign equivalents.