Florida Central Railroad Orlando (interchange
with CSX) via Toronto (branches to Winter Garden and Forest City)
and Tavares (branch to Sorrento) to Umatilla; total length of line
about 68 miles (109 km)

Florida East Coast
Railway Jacksonville (interchange with CSX, Frontier Rail and
Norfolk Southern) via Cape Canaveral Junction (branch to Kennedy
Space Center and the Cape Canaveral Air Force Base), Fort Pierce
(interchange with United States Sugar) and West Palm Beach (interchange
with CSX) to Port of Miami; total length of line about 396 miles
(637 km)

Kennedy Space Center Railroad
Cape Canaveral Junction (interchange with Florida East Coast) to
the Kennedy Space Center and the Cape Canaveral Air Force Base.
Total length of all tracks about 45 miles (72 km). Currently out of use
but proposed to form part of Port Canaveral Rail Extension, see below
(No website located at present, but see historical article)

Mosaic
Company owns and operates the line from an interchange with CSX near
Streamsong Resort to Four Corners Mine (Website contains no
information relating to railway)

Orlando Utilities Commission performs some switching at
its Curtis H Stanton Energy Center, interchanging with CSX;
however, most of the rail traffic arriving at the plant is formed of
block trains which do not require switching (Website contains no
information relating to railway)

Port
Canaveral Rail Extension proposed railway serving Port Canaveral.
From Cape Canaveral Junction on the Florida East Coast Railway it would
utilize about 17 miles (27 km) of the former NASA Railroad in the
Kennedy Space Center to connect with a new 11 mile (18 km) railway
serving the port.

Oneco (interchange with CSX) via Sarasota (branches to Matoka
and Fruitville) to Venice

Arcadia (interchange with CSX) via Fort Myers to Vanderbilt
Beach

Total length of line over 100 miles (160 km)

Talleyrand Terminal Railroad switching railroad in
Talleyrand Docks, Jacksonvile. Interchanges with CSX and Norfolk Southern.
Total length of line about 2 miles (5 km)

United States Sugar Corporation separately operated but
connected networks:

South Central Florida Express Sebring (interchange
with CSX) via Clewiston (interchange with private network) and Keela
(branch to Okeelanta), Bryant (interchange with private network) and
Marcy (interchange with CSX) to Fort Pierce (interchange with Florida
East Coast); total length of track about 162 miles (260 km)

Private network extensive network of branches
connecting sugar plantations to refineries and interchange with
South Central Florida Express, arranged in two groups: about 38 miles
(61 km) around Clewiston, and about 36 miles (58 km) around Bryant