Interpretive Summary: An upland cotton germplasm, NM24016 derived from an interspecific cross between Gossypium hirsutum and G. barbadense was used to cross with Pima 3-79 to develop a recombinant inbred line (RIL) population. A total of 166 markers, consisting of SSRs, RAPDs, STSs, and SRAPs were developed for the mapping population. Twenty-eight linkage groups were constructed from 142 markers. Comparative marker mapping between F2 and RIL populations indicated a linkage map expansion in the RIL population, which strongly supports the strategy to develop the RIL population for more accurate genetic mapping. Other 24 markers that are polymorphic between the two parents did not segregate in the RIL population, indicating selective elimination on the related chromosomal regions.

Technical Abstract:
An upland cotton germplasm, NM24016 derived from an interspecific cross between Gossypium hirsutum and G. barbadense was used to cross with Pima 3-79 to develop a recombinant inbred line (RIL) population. A total of 166 markers, consisting of SSRs, RAPDs, STSs, and SRAPs were developed for the mapping population. Twenty-eight linkage groups were constructed from 142 markers. Comparative marker mapping between F2 and RIL populations indicated a linkage map expansion in the RIL population, which strongly supports the strategy to develop the RIL population for more accurate genetic mapping. Other 24 markers that are polymorphic between the two parents did not segregate in the RIL population, indicating selective elimination on the related chromosomal regions.