This high performance widefield microscope system can perform complex and rapid
acquisition tasks such as time lapse, fura-2 and other ratio imaging, rapid
filter changes, red light DIC with fluorescence (dual mode) and color capture of transmitted light samples. Optical modes of operations
are transmitted light, DIC (Nomarski), Phase contrast & Fluorescence.
A high
sensitivity CCD camera as well as an intensified CCD camera are available. See
facility personnel about these functions before using the microscope.

Book
this scope:

If you not been trained or received an orientation on this scope please
contact facility personnel.

Do not look down the eye pieces when changing dichroics on the
microscope carousel. Some filter sets do not have a barrier filter
(emission)
installed and the intense light can be harmful to vision.

Extreme care must be taken not to damage the intensified camera with bright
light. This camera is usually not attached to the system (power is
disconnected) in order to
protect it from damage.

Please ensure that the dual head module is set to the "1" position
before starting to use this system, and when you finish up.

Notes:

Running the System: This is a complex setup with several different
options which require changing parts. Please contact
facility personnel about
these functions before using the microscope or if you have problems or
questions.

Press Focus button, which starts rapid acquisition of an image to
the display window (03)

Increase or decrease exposure time in order to get an image (03)

for fluorescence it may be desirable to increase camera gain to
maximum (04) and decrease exposure time in order to make focusing easier.
Note that image may be noisy. Black level should not be changed.

Focus while viewing image on monitor

For multi-color imaging: Click on the "Red", "Green" or "Blue" tabs to change which channel is
displayed in the Focus window
NOTE: The Focus image is always black and white

[Insert Screen Image with
tabs highlighted here]

If desired, return camera gain to 41 (04) and increase exposure time (03) to
reduce noise

* These filters are not kept on the system. Please contact a
facility director if you require these filters.** Filters not installed in the DG-4 are found in the plastic drawer labeled
"DG-4 Filters" to the left of the microscope nook. Please
keep filter sets together (ie, CFP+YFP, FURA-2, etc)