Application Information: Adiponectin antibody

WB: Use at a dilution of 1/5000 - 1/10,000 using ECL. Reacts with the 30 kDa adiponectin protein. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at room temperature. ELISA, direct and indirect: 1:5,000-1:10,000. Optimal dilutions/concentrations should be determined by the end user.

Form Supplied

Liquid

Concentration

1
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Purified immunoglobulin

Purification Note

From polyclonal serum

Specifications: Adiponectin antibody

Full Name

adiponectin, C1Q and collagen domain containing

Background

Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase, leptin, and adipocyte complement related protein of 30 kDa (Acrp30). Acrp30 is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, mRNA levels of Acrp30 have been shown to be induced over 100-fold. Studies indicate that Acrp30 enhances the ability of sub-physiological levels of insulin to suppress glucose production, thus linking adipose tissue to whole body glucose regulation. Acrp30 function appears to be regulated by phosphatidylinositol 3 kinase (PI3K) since Acrp30 secretion is blocked by pharmacologic inhibitors of this kinase. mRNA levels of Acrp30 have been shown to be significantly reduced in adipose tissue of obese patients with Type 2 diabetes relative to control subjects. Structural similarities to TNF alpha suggests that Acrp30 may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Because Acrp30 is down-regulated in various forms of obesity and its structural similarity to TNF alpha, it is currently being investigated as an important regulator of whole body energy homeostasis.