1Department of Medicine, Kangbuk Samsung hospital, Sungkyunkwan University School of Medicine

2Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea, Republic Of

Abstract

Background Metabolomics is the study of unique chemical imprints that represent specific cellular processes in a cell, tissue, organ or organism. Synovial fluid in pathologic conditions reflects the diseased process and its distinctive metabolite profiles could facilitate the diagnostic ability and the understanding of disease state.

Objectives The aim is to investigate the metabolites of synovial fluid in patients with RA and OA and to identify characteristic biomarkers to differentiate two diseases.

Methods Synovial fluid samples were obtained from patients with RA (n = 18, 17 females, mean age 50.3 ± 13.9 yr, disease duration 7.9 ± 6.8 yr) and OA (n = 11, 10 females, mean age 60.9 ± 8.4 yr, disease duration 2.8 ± 4.7 yr). The extracted metabolites from synovial fluid were analyzed by gas chromatography/time-of-flight mass spectrometry (GC/TOF MS). The identified metabolites from synovial fluid extracts of RA and OA were then subjected to multivariate statistical analysis by orthogonal partial least squares discriminant analysis (OPLS-DA): R2 indicates the fitting ability of total variation and Q2designates the validity of discrimination. Both have range from 0 to 1, where the higher R2 or Q2connotes a model with the higher predictive and discriminative value. Values of variable importance for projection (VIP) greater than 1 from OPLS-DA were used to identify potential biomarkers and the significance was defined by Welch’s t-test with level of p < 0.01.

Statistics from Altmetric.com

No Altmetric data available for this article.

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.