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DNA Replication

Replication Facts:

2 Replication Facts Remember, your body cells can divide by mitosis so your body can grow and repair wounds. Remember, parents must create sex cells by meiosis to pass their DNA (half of it!) to their children. DNA must be copied before mitosis and before the first division of meiosis.

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DNA Replication Step 3 : DNA nucleotides in the cell match up with each side of the “unzipped” DNA. Each “unzipped” old strand forms a template (a model) for a new strand.

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Step 4 : Each “old’ strand forms a template for a “new” strand. Two identical DNA molecules form, one strand is “new”, one strand is “old”. This is referred to as semi-conservative replication . “old” (original) strand “new” strand , identical sequence to the original DNA Replication

DNA Helicase:

DNA Helicase DNA helicase – is a special protein called an enzyme which unwinds or “unzips” the DNA by breaking the hydrogen bonds between the bases. Unzipping the DNA forms a region called a replication fork. Replication Fork : “Y-shaped region” in DNA molecule where helicase separates the two strands. Replication Fork DNA Molecule

DNA Polymerase:

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DNA Replication http://www.youtube.com/watch?v=zdDkiRw1PdU

DNA Polymerase:

13 DNA Polymerase DNA polymerase initially makes about 1 in 10,000 base pairing errors . DNA polymerase proofreads and corrects many of these mistakes. For example, if an adenine pairs with a cytosine instead of a thymine, DNA polymerase can repair the error by removing the mispaired cytosine and replacing it with a thymine. The new error rate for DNA that has been proofread is 1 in 1 billion base pairing errors.

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17 Some errors escape repair. When mistakes in DNA replication do occur, the base sequence of the newly formed DNA differs from the base sequence of the original DNA. A change in the nucleotide sequence of a DNA molecule is called a mutation . Mutations can be harmful , helpful , or neutral . You will learn more about the effect mutations can have on an organism later in the chapter. Mutations