Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

This kit is for the detection of Rat Collagen IV. No significant cross-reactivity or interference between Collagen IV and analogs was observed. This claim is limited by existing techniques therefore cross-reactivity may exist with untested analogs.

Description:

LS-F27369 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat Collagen IV in samples of Cell Culture Supernatants, Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of Collagen IV as low as 0.1 nanograms per millilter.

Custom Kits:

All LSBio Custom kits have been predesigned and all necessary components, including antibodies and standards have been identified. Upon receiving a custom kit order, the kit will be assembled and quality control tested before being shipped out. Kit assembly and testing typically takes 4 to 6 weeks. In most cases the final kit is based on the Sandwich assay principle, with a few being Competitive EIA based. Specifications such as Range, Sensitivity, and Precision are defined upon completion. In the event that the custom kit cannot be successfully developed with 6 weeks of the order date, the customer will be notified and the order canceled at no cost.

Sample Type:

Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates

Format:

96-Well Strip Plate

Detection:

Colorimetric - 450nm

Measurement:

Quantitative

Detection Range:

1 - 25 ng/ml

Sensitivity:

0.1 ng/ml

Assay Time:

1.5 h

Precision:

Intra-Assay: CV%<9% Inter-Assay: CV%<10%

Quality Assurance:

Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping. ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Kit Components:

Adhesive Plate Sealers

Coated 96-well Strip Plate

Standard A

Standard B

Standard C

Standard D

Standard E

Standard F

Balance Solution

HRP-Conjugate

Wash Buffer (100x)

Substrate A

Substrate B

Stop Solution

Storage:

Store at 2-8°C.

Restrictions:

For research use only.

About Collagen IV

Description: Type IV Collagen (ColIV or Col4) is a type of collagen found primarily in the basal lamina. The collagen IV C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause the collagen to form in a sheet, the form of the basal lamina.