Littleton Museum offers visitors a unique link between the past and present. Located on 40 acres adjacent to Ketring Lake, the museum encompasses three exhibition galleries, a children's interactive gallery, research center, and two 19th-century living history farm sites.

The Permanent Gallery exhibits a comprehensive historical look at Littleton from pre-history to the present through graphics, photography, and artifacts from the museum's collections.

The Fine Arts Gallery hosts original art and photography exhibits sponsored by the Littleton Fine Arts Board. The Changing Gallery displays temporary and traveling exhibits from the Smithsonian Institution, other museums, and the Littleton Museum Collections.

The 1860s homestead farm and the 1890s urban farm represent how people lived and worked during the early years in Littleton and the South Platte Valley. Museum staff and volunteers, dressed in period-appropriate costumes, work on the farm sites, run the blacksmith shop, and teach in Littleton's first one-room school, offering a "living history" perspective to the museum visitor. The animals (oxen, mules, chickens, turkeys, sheep, etc.) located on the farm sites are considered livestock and are not suitable for petting or feeding by visitors.

The Research Center containing the museum's non-circulating collection of books, manuscripts, maps, and photographs focused on Littleton and the surrounding area is open to the public by appointment only. Staff and volunteers are available to assist with research.

In keeping with the museum's mission, the museum gift shop sells educational toys, books for all ages, and special gifts for friends and family.

The Littleton Museum is accredited by the American Association of Museums and is the first museum in Colorado to be recognized as an Affiliate of the Smithsonian Institution.