Summary:
CYP83A1 metabolizes aldoximes derived from chain-elongated methionine. The Km for these aliphatic aldoximes is in the range of 20 to 150 uM [Naur03].

Both CYP83A1 and CYP83B1 metabolize phenylacetaldoxime. However phenylacetaldoxime is a better substrate for CYP83B1. The Km of CYP83A1 for phenylacetaldoxime is 3-fold higher than that of CYP83B1. The catalytic efficiency (Kcat/Km) of CYP83B1 is 6 times higher than that of CYP83A1 [Naur03].

The reaction is thought to involve a carbon-sulfur lyase to form the thiohydroximate from the intermediate alkylthiohydroximate [Wittstock02].