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What CPC can bring to your natural compounds purification?

2.
GILSON BY THE NUMBERS
Founded by Dr. Warren Gilson in 1957
# OF EMPLOYEES # OF
ORGANIZATIONS
# OF PATENTS
GRANTED
IN THE
GLOBAL TOP
We’re globally accessible to our
customers through a complete
network of experts.
From product to customer-facing
organizations, we’re investing in
the life science industry.
Since 1957, we’ve been making
lab life easier for researchers by
pioneering key advances to our
lab devices.
Our customers have made
us a top reference company in the
analytical & life science industries.
550+ 14 750+ 100

5.
CPC DEFINITION
Centrifugal Partition Chromatography also names Counter Current Chromatography
Preparative, pilot, and industrial scale liquid/liquid partition chromatography for purification of all kinds of molecules in
solutions with the specificity to do not use solid support as silica
Silica free preparative chromatography

8.
CPC SETUP
A CPC system is a preparative LC column analogous to a prep HPLC column or flash cartridges.
A preparative-scale pump and an injector are required, and an optional detector and collector can be added.
CPC Setup

12.
CPC GENERAL PRINCIPLE
• Liquid-liquid partition chromatography
o two immiscible liquid phases
o Done by mixing two or more solvents
• One phase is the stationary phase: maintained by
centrifugal force
• The other phase is the mobile phase: pumped
through the liquid stationary phase
• Compounds separated according to their partition
coefficient KD between the phases
Same principle as prep HPLC but no solid support
Silica
in
HPLC
Biphasic
System in
CPC

13.
CPC GENERAL PRINCIPLE
KD > 1
KD = 1
KD < 1 lower
upper
D
A
AK
][
][=
For a substance dissolved in a biphasic system, at a fixed temperature, the
partition coefficient can be defined as follows:
Concentration of A in
upper phase
Concentration of A in
lower phase
Separatory
funnel
Lighter upper
phase
Heavier lower
phase

14.
CPC GENERAL PRINCIPLE
1. Separatory funnels in series
2. Loading of stationary lightest phase in all separatory funnels
3. Injection of sample (mixture of three molecules)
4. Loading of mobile phase in the first funnel, shaking, separation
5. Transfer of lower mobile phase from the first to the second funnel, loading of fresh mobile phase in the first
funnel
6. Step 5 repeat until the last funnel
Heaviest phase: mobile
phase
Time
KD > 1
KD = 1
KD < 1

15.
CPC GENERAL PRINCIPLE
1. Separatory funnels in series
2. Loading of stationary lightest phase in all separatory funnel
3. Injection of sample (mixture of three molecules)
4. Loading of mobile phase in the first funnel, shaking, separation
5. Transfer of lower mobile phase from the first to the second funnel, loading of fresh mobile phase in the first
funnel
6. Step 5 repeat until the last funnel
Heaviest phase: mobile
phase
KD > 1
KD = 1
KD < 1
Time

40.
GILSON PURIFICATION : CONTRACT SERVICES
Proposed our know-how and expertise in preparative and industrial chromatography to purify and / or
enriched molecules of interests from complex mixtures as biological matrices, natural extract, synthetic
mixture or fermentations booth
Proposal:
 Overall supply from feasibility to production through industrial intensification of the process
 Use of innovative techniques as Centrifugal Partition Chromatography to reduce costs and solve
problems related to the use of traditional techniques
 Full project support to provide turnkey solutions instrument / method.
 Unlock problems related to the use of traditional techniques