The ovary is trilocular with axile placenta, and the ovules are bitegmic, tenuinucellate, and anatropous.

A gradual increase in the content of the active and storage forms of the hormones from the ovary to the ovule was revealed.

Effect of Estradiol Dipropionate on Protein Synthesis in Oocytes and Ovary of the Sea Urchin Strongylocentrotus intermedius at D

We studied protein synthesis in the oocytes and ovary of the sea urchin Strongylocentrotus intermedius at different stages of the reproductive cycle after treatment with estradiol dipropionate.

After estradiol dipropionate treatment, the inhibitors of protein synthesis, puromycin and actinomycin D, decreased the intensity of 3H-leucine incorporation in the oocytes and protein synthesis in the ovary.

The ovary transplant experiments indicate that CHICO in the ovary is necessary for vitellogenic maturation.

The hormonal control of the hypothalamo-pituitary-ovary(ocular graft)axis was investigated in the rabbits.Autotransplantation of the ovary to theanterior chamber of the eye was performed immediately after bilateral ovariec-tomy.The FSH,LH,cAMP and Progesterone content in the blood of the controlrabbits and the rabbits with ocular ovarian grafts were determined by RIA andcompetitive protein-binding assay method.The experiments disclosed thatafter exogenous intravenous administration of 5 μg/kg of GnRH,there was...

The hormonal control of the hypothalamo-pituitary-ovary(ocular graft)axis was investigated in the rabbits.Autotransplantation of the ovary to theanterior chamber of the eye was performed immediately after bilateral ovariec-tomy.The FSH,LH,cAMP and Progesterone content in the blood of the controlrabbits and the rabbits with ocular ovarian grafts were determined by RIA andcompetitive protein-binding assay method.The experiments disclosed thatafter exogenous intravenous administration of 5 μg/kg of GnRH,there was a gra-dual increment in both FSH and LH content at 30,60 and 240 mins intervals,with basically similar pattern.When the graft-bearing animals were mated withmales,the FSH,LH and cAMP content in the blood at 30,60,120 and 240 minsintervals also showed conspicuous increase of similar magnitude in both the con-trol and the experimental,groups of animals.The characteristic hemorrhagic spots on the surface of the graft,designatedas the ovulatory response,might be initiated by mating,copper acetateHCG orGnRH.The blood Progesterone content measured on the second,fourth andsixth day after the ovulatory response increased 5-6 times above normal.Further-more,the ovulatory response could be suppressed by the administration of theantifertility solution no.1 or 18-megestrol.Taken all these experimental datatogether,it is believed that the integrity of the hypothalamo-pituitary-ovary(ocular)axis is essentially normal as compared with that of the normal.The transparent cornea(‘window’)furnishes a very convenient means of carry-ing out direct and long-term observations,particularly useful for screening an-tifertility drugs and in the delineation of the relations of the different componentsof the axis.

The effect of tyrosine on the stero(?)dogenesis and cAMP accumulation ot the granulosacells and theca tissues in the rat was studied by the incubation method.To obtain thegranulosa cell suspensions and theca tissues,the preovulatory follicles of rats were manuallyisolated 42 hours after a single injection(SC)of HCG.After isolation,granulosa cellsuspensions were made and co-cultured with the theca tissues for 4 hours in KRBGAL buf-fer.Then,the progesterone and estrogen contents in the incubates were ascertained...

The effect of tyrosine on the stero(?)dogenesis and cAMP accumulation ot the granulosacells and theca tissues in the rat was studied by the incubation method.To obtain thegranulosa cell suspensions and theca tissues,the preovulatory follicles of rats were manuallyisolated 42 hours after a single injection(SC)of HCG.After isolation,granulosa cellsuspensions were made and co-cultured with the theca tissues for 4 hours in KRBGAL buf-fer.Then,the progesterone and estrogen contents in the incubates were ascertained byRIA,while the cAMP accumulation in them was determined by the competitive proteinbinding assay.These preliminary experiments indicated that tyrosine,when added tothe incubation media containing HCG,conspicuously antagonized the effect of HCG on pro-gesterone,estrogens and cAMP production,with the progesterone and cAMP contents inthe incubates decreased from 115.4 ± 14.5 ng/ml to 56.6 ± 4.6 ng/ml and from 24.8± 1.4 pmol/ml to 13.8 ± 0.8 pmol/ml,respectively,while that of the estrogens increasedfrom 3.29 ± 0.40 ng/ml to 20.47 ± 2.10 ng/ml.It may therefore be postulated that tyrosine may block the action of HCG at the ovarianlevel.The mechanism of the antagonistic effect of the tyrosine molecule remains to beclarified.

A 1 cm segment of oviductal isthmus was taken,1 to 4 cm from the junctura,from ananesthetized rabbit.The sample was soaked in a 38℃ double glass chamber bath,whichcontained Krebs' solution bubbled with a mixture of 9.5% O_2 and 5% CO_2.The lower andof the tissue was attached to a fixed hook at the base of the bath and the top end was con-nected to a strain gauge.The muscular contraction of oviductal isthmuses from five groupsof animals in different hormonal states(estrous rabbit,ovulated rabbit,ovariectomized...

A 1 cm segment of oviductal isthmus was taken,1 to 4 cm from the junctura,from ananesthetized rabbit.The sample was soaked in a 38℃ double glass chamber bath,whichcontained Krebs' solution bubbled with a mixture of 9.5% O_2 and 5% CO_2.The lower andof the tissue was attached to a fixed hook at the base of the bath and the top end was con-nected to a strain gauge.The muscular contraction of oviductal isthmuses from five groupsof animals in different hormonal states(estrous rabbit,ovulated rabbit,ovariectomized rab-bit,immature rabbit and immature rabbit with EB〔Estradiol Benzoate〕was recorded con-tinuously for 1-2 hours,using the polygraph.The optimal condition of the dose-response relationship was studied.The isthmic moti-lity in all five groups showed a common tendency:In the first 30-minute period,there occurredan “adaptive phase”in which the tension of the smooth muscle increased progressively;inthe following 30-50 minute peroid there was a“steady phase”,during which the tension,frequency and amplitude of the spontaneous single contractions became relatively steady.Beyond this period,the isthmic contractility varied significantly according to the differenthormonal states of the rabbits.Firstly,the contractility pattern of the estrous and ovulatedrabbits displayed a series of burst-like contractions,but no such phenomena could be observedin the immature or ovariectomized rabbits.Secondly,the order of the contraction tensionin the five groups was:ovulated rabbit group>estrous rabbit group>immature rabbitwith EBgroup>ovariectomized rabbit group>immature rabbit group.Thirdly,the rate oftension increase(RTI)revealed that the ovulated rabbit group showed a rapid change inRTI,sustained for about 30 minutes.However,the other four groups had a dramatic increascin RTI in the first 10 minutes,but afterwards the change became less rapid.It is suggested that the variation in the criteria mentioned above is correlated to the ova-rian hormonal level,which might influence the protein synthesis in the smooth muscle,thesize of the fibers,the glycogen content and the sensitivity of the muscle NE(Norepinephrine)receptor.In addition,a repeatable-dose response result could be obtained by this in vitro methodduring the“steady phase”in the determination of the sensitivity response of the oviduc-tal isthmus to NE.