PALM and STORM : unlocking live-cell super-resolution

Abstract:

Live-cell fluorescence light microscopy has emerged as an important tool in the study of cellular biology. The development of
fluorescent markers in parallel with super-resolution imaging systems has pushed light microscopy into the realm of molecular
visualization at the nanometer scale. Resolutions previously only attained with electron microscopes are now within the grasp of
light microscopes. However, until recently, live-cell imaging approaches have eluded super-resolution microscopy, hampering it
from reaching its full potential for revealing the dynamic interactions in biology occurring at the single molecule level. Here we
examine recent advances in the super-resolution imaging of living cells by reviewing recent breakthroughs in single molecule
localization microscopy methods such as PALM and STORM to achieve this important goal.