Jump-In™ Cell Engineering Platform

Whether you are an experienced cell engineer, or need engineered cells for your projects, the Jump-In™ cell engineering platform can accelerate your projects by enabling you to generate engineered cell lines in much less time than traditional methods. The Jump-In™ technology helps accelerate stable cell line development due to the targeted integration of genetic material into specific integration sites using PhiC31 integrase.

Unlike the better-known recombinases such as Cre and Flp, PhiC31 integrase catalyzes recombination between two nonidentical sites and lacking a corresponding excision enzyme makes the integration unidirectional and virtually irreversible. In addition, combining PhiC31 integrase with R4 integrase allows for isogenic expression from a defined genomic locus, providing the ideal solution for comparative analysis of gene families, isoforms or orthologs. Depending on your needs and experience, we offer multiple options for accessing Jump-In™ technology, allowing you to choose the method that best utilizes your available resources and fits your desired timelines.

Jump-In™ Fast Gateway® System

Jump-In™ Fast Gateway® System allows you to generate stable clones in just two weeks by introducing your genetic material into your cell background using PhiC31 integrase to produce a highly positive pool of retargeted cells. Screen only 10 clones to find one with your ideal expression level.

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Jump-In™ TI™ Gateway® System

Jump-In™ TI™ Gateway® System enables the creation of isogenic cell lines that express your gene of interest by having you initially generate a retargetable platform cell line containing the R4 attP sites in your preferred cell background.

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Jump-In™ Parental Cell Line Kits

Jump-InTM parental cell line kits allow you to generate isogenic cell lines by providing a parental (platform) cell line with a single R4 attP site ready to be retargeted with your genetic material.

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Jump-In™ Custom Services

Reduce assay development and cell engineering project timelines by outsourcing your cell line development to our Custom Services team.

Let us craft a Jump-InTM parental cell line or the final engineered cell line meeting your defined specifications; while a dedicated project manager provides frequent updates on project milestones.