Obtain High Yields of Transfection-Grade Plasmid DNA—Fast!

Ensure Higher Transfection Efficiency

Transfection efficiency depends on many factors, including DNA quality and purity. Anion-exchange columns (such as NucleoBond Xtra) provide the purest DNA and the best transfection results. However, DNA purified with other brands of anion-exchange DNA purification columns cannot match the purity and transfection efficiency of DNA purified with NucleoBond Xtra DNA purification columns.

Figure 1. Plasmid DNA for a gephyrin-GFP fusion was purified with NucleoBond Xtra and with a plasmid DNA purification kit from Competitor Q. HEK 293 cells were transfected with plasmid DNA and incubated for 48 hr before visualization by fluorescent microscopy. NucleoBond Xtra gave higher transfection efficiency.

Purify More DNA in Less Time

NucleoBond Xtra columns purify up to 2–5 times more DNA than other columns, in up to 60% less time. Plasmid DNA was isolated following each manufacturer's protocol, using the maximum culture volume and high plasmid content. Plasmid DNA yield was determined after DNA precipitation

NucleoBond Xtra
Beats other Anion-Exchange Purification Columns

Midiprep Kit*

Yield (µg)

Time (min)

NucleoBond Xtra Plus*

520

28

Competitor Q HiSpeed*

335

47

NucleoBond Xtra

580

68

Competitor Q

260

185

Competitor I

380

131

NucleoBond Xtra
Beats Silica Membrane Purification, Too

Midiprep Kit

Yield (µg)

Time (min)

NucleoBond Xtra Plus*

520

28

Competitor S

300

37

Competitor P

90

41

* These kits use a desalting tool for DNA precipitation

Purify Endotoxin-Free DNA for Sensitive Cells

Endotoxins are components of the outer membrane of Gram-negative bacteria. They are toxic to cells grown in culture, and unfortunately they can be found in any non-sterile environment. NucleoBond Xtra EF kits use a patented procedure to reduce endotoxins to a very low level (0.05 EU/µg) that is suitable for the transfection of highly sensitive cells such as primary neurons and for lentivirus production.