Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

pluripotent teratocarcinoma

Gender

male

Strain

129/Sv

Applications

The cells can be maintained in an undifferentiated state by frequent subculture and the use of a fibroblast feeder cell layer (see ATCC CRL-1503).

In the absence of a feeder layer, the cells are encouraged to form aggregates which differentiate forming outer endodermal layers which encapsulate the aggregates.

Aggregates (embryoid bodies) can be encouraged to differentiate further by keeping them in suspension for 5 or more days.

Tested and found negative for ectromelia virus (mousepox).

Derivation

The SCC-PSA1 line was isolated from secondary cultures of the OT/5568 transplantable tumor.

Clinical Data

male

Comments

NOTE - NO LIVE CULTURES CAN BE SENT.

The cells can be maintained in an undifferentiated state by frequent subculture and the use of a fibroblast feeder cell layer (see ATCC CRL-1503).

In the absence of a feeder layer, the cells are encouraged to form aggregates which differentiate forming outer endodermal layers which encapsulate the aggregates.

Aggregates (embryoid bodies) can be encouraged to differentiate further by keeping them in suspension for 5 or more days.

Collect the bodies from a bacteriological dish and plate them in fresh medium in a tissue culture dish without feeder layer or gelatin.

A somewhat variable percentage will attach.

Change the medium every 2 to 3 days.

Migration of the endodermal layer should begin within 24 to 36 hours after plating.

Do not dislodge cells when changing the medium.

The SCC-PSA1 line was isolated from secondary cultures of the OT/5568 transplantable tumor.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculture every 3 days to maintain in an undifferentiated proliferative state.

Culture Conditions

Temperature: 37.0°C

Permits

These permits may be required for shipping this product:

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.