The Alpha SureFire®Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of phosphoproteins from cells, combined with the measurement of another protein, phospho or total. This Alpha Multiplex measurement is carried out in the same assay plate well from a single sample of cell lysate, and is achieved by the use of two types of Alpha Acceptor beads that emit at distinct wavelengths (545nm and 615nm).

Overview

The Alpha SureFire Ultra Multiplex p-Akt 1/2/3 (Ser473) + p-ERK assay kit is used to measure both the phosphorylation of both Akt and ERK in cellular lysates. The kit measures phosphorylation of Akt 1/2/3 on Ser473, and phosphorylation of ERK 1/2 on Thr202/Tyr204. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Akt (Ser473) and ERK activation by either recombinant or endogenous receptors, and can be applied to primary cells.