INDOLE NITRATE MEDIUM

Technical Data #2086a / 2013.06.23

Indole Nitrate Medium is a semi-liquid medium used to determine indole production and nitrate reduction by a wide variety of organisms. Even this medium is capable of supporting growth of aerobes and anaerobes organisms, is not recommended for the Enterobacteriaceae indole production due to the reduction of nitrate to nitrite which prevents detection of indole.

FORMULAin grams per liter purified filtered water

Casein Peptone

20,0

Sodium Phosphate, dibasic

2,0

Dextrose

1,0

Potassium Nitrate

1,0

Agar

1,0

pH 7,2 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONSThis medium is for IN VITRO diagnostic use only.

STORAGEStore prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.

SIGN OF DETERIORATIONMedia should not be used if the expiry date has passed. prepared media should not be used if there are signs of contamination (evaporation or discoloration). Do not use dehydrated media if it is caked.

DIRECTIONSSuspend 25 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Mix thouroughly and dispense into culture tubes. Sterilize at 121º C for 15 minutes.