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Description

For the first time, you can obtain the benefits of Ambion®Silencer® Select siRNAs in a convenient pre-plated, library format. These siRNA collections enable medium and high throughput analysis of phenotypes resulting from inhibition of large sets of targets from specific functional classes.

• Ready-to-ship collections of Ambion® Silencer® Select siRNAs to popular human gene classes• Three individual siRNAs per target provided in a convenient 96-well format• Designed with an all new algorithm that yields better, more consistent knockdown• Novel chemical modifications reduce off-target effects by up to 90%• Up to 100 fold more potent than currently available siRNAs• Demonstrated improvements in consistency and reliability of phenotypic results

What are Silencer® Select siRNAs?Silencer® Select siRNAs are the next generation Ambion® siRNAs. These siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNAs with unrivalled efficacy, potency, and specificity. In siRNA screening applications, the result is fewer false negatives due to poor silencing and fewer false positives due to off target effects. In other words, with Silencer® Select siRNAs you get cleaner, more consistent data from your primary screen, so you expend less time and resources to find true, validated targets. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.

Human Genome siRNA Library(Comprised of Part Numbers: 4397922 + 4397924 + 4397924)Increasingly, researchers are performing genome scale surveys of gene function. The Silencer® Select Human Genome siRNA Library V4 is ideal for this purpose. Three unique, non-overlapping siRNAs are provided for each of 21,584 human targets. The siRNAs targeting the “druggable" portion of this library are arranged by gene functional class to enable easy screening of important gene subsets such as kinase, phosphatase, GPCR, etc. This siRNA library is supplied in 384-well plates.

Human Druggable Genome and Human Extended Druggable Genome siRNA LibrariesWith siRNAs targeting the most therapeutically relevant human genes and conveniently grouped by target gene class, the Silencer® Select Human Druggable Genome siRNA Library V4 and Silencer® Select Human Extended Druggable Genome siRNA Library V4 are popular choices for many researchers. The druggable genome set targets 9031 genes, whereas the extended druggable genome collection targets those genes plus the "Extension Set" for a total of 10414 genes, including transcription factors. Please contact us for the complete list of targets. These libraries are available in both 96 and 384 well plate formats.

Individual siRNAs for Enhanced Data ReliabilitySilencer® Select siRNA Libraries feature three individual siRNAs for each target. Screening with three siRNAs per gene significantly decreases both false positive and false negative rates, resulting in enhanced confidence in RNAi screening data, reduced chance of missing important genes, and less time spent following up on false positive hits from the screen.

These libraries contain 0.25 nmol of each siRNA, sufficient for 500 transfections (at 5 nM siRNA, 100 µl transfection volume). The siRNAs are supplied dried into the wells of 96 well plates. One column of each 96 well plate is left empty to allow for the addition of controls at the time of use. The libraries are delivered with full siRNA sequence information, as well as the exon targeted and each targeted gene's symbol, full name, aliases, RefSeq number, and Entrez Gene ID, plus results from any validation experiments done using the included siRNAs.

Silencer® Select siRNA Libraries to these or any other list of human genes can also be provided with 1, 2, or 5 nmol of each siRNA. Custom formatting is available with these larger sizes. If you would like a gene list or other information, or would like to discuss your research with one of our technical experts, contact your local Sales Representative or e-mail us at libraries@ambion.com.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Druggable Target:

Ion Channels

Format:

96-well plate

Product Size:

1 set

Species:

Human

Shipping Condition:

Room Temperature

Contents & storage

Contains a total of 1014 unique siRNAs (0.25 nmol) targeting each of 338 human ion channel genes. Total of (12) 96-well plates: (9) plates with 88 siRNAs each. (3) plates with 74 siRNAs each. See the accompanying CD for annotation information and siRNA details. Store at or below –20 ° in a non-frost-free freezer.