Carotenoids are very abundant in nature and their consumption has numerous positive health outcomes. There are two known enzymes that are responsible for cleaving carotenoids in to their metabolites. β-carotene is cleaved by carotenoid-15,15'-oxygenase (CMO-I), to eventually form biologically-active retinoids. Lycopene is a non-provitamin A carotenoid and the most abundant carotenoid in tomatoes. Lycopene is a poor substrate for CMO-I, but our lab along with other has proposed that the enzyme carotenoid 9'10'-monoxygenase (CMO-II) can oxidatively cleave lycopene. In order to assess the impact of dietary carotenoids and their metabolites on lipid metabolism in female mice, CMO-I KO, CMO-II KO or wild type (WT) mice, 29-31 weeks old, were fed the following AIN-93G based diets for either 4 or 30 days: lycopene beadlet, 10% tomato powder, and their respective controls. We hypothesized that mice lacking either carotenoid cleavage enzyme would have altered serum and hepatic lipids compared to WT mice while lycopene or tomato powder might modulate these effects. Our data demonstrate that a lack of CMO-I or CMO-II altered reproductive organ weights and lipid status, and feeding carotenoid-containing diets had modest impact on lipid metabolism in these mice.