Sole

Hello everyone, I want to know if you have performed a 2D gel and transfered the proteins in the gel to a PVDF membrane to incubate then with anti-Phosphoprotein ab.I need to do something like this with cerebellum tissue and I am looking for a protocolThank you very much!

Hello,You can just run a standard 2D gel as described here in the forum. After running the gel, you can continue with any standard blotting procedure.In our hands a wet transfer gives best results. Which format are you planning to run?