Anwendungsinformationen

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hFlt3-ligand by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hFlt3-ligand. To detect hFlt3-ligand by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human Flt3-ligand (LS-C104826) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hFlt3-ligand. Western Blot: To detect hFlt3-ligand by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFlt3-ligand is 1.5-3 ng/lane, under either reducing or non-reducing conditions.