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Thursday, February 21, 2013

Cell Explorer™ Live Cell Labeling Kit (Green Fluorescence)

AAT Bioquest Cell
Explorer™ fluorescence imaging kits are a set of tools for labeling
cells for fluorescence microscopic investigations of cellular functions.
The effective labeling of cells provides a powerful method for studying
cellular events in a spatial and temporal context. This particular kit
is designed to uniformly label live cells in green fluorescence. The kit
uses non-fluorescent Calcein Green™ that becomes strongly fluorescent
upon entering into live cells. Calcein Green™ is a hydrophobic compound
that easily permeates intact live cells. The hydrolysis of the
non-fluorescent Calcein Green™ by intracellular esterases generates the
strongly fluorescent hydrophilic Calcein Green™ fluorophore that is
well-retained in the cell cytoplasm. Cells grown in black-walled plates
can be stained and quantified in less than two hours. It can be readily
adapted for high-throughput assays in a wide variety of fluorescence
platforms such as microplate assays, immunocytochemistry and flow
cytometry. It is useful for a variety of studies, including cell
adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis
and cytotoxicity. The kit provides all the essential components with an
optimized cell-labeling protocol. It is suitable for proliferating and
non-proliferating cells, and can be used for both suspension and
adherent cells.

Stratech Scientific is a distributor of high quality, competitively
priced, reliable products for research laboratories throughout the UK
and Europe. Please contact us to find out which ranges we can supply in
your country.