Human α2-Smooth muscle actin (α2-SMA) ELISA Kit

Product Information

Product name

Human α2-Smooth muscle actin (α2-SMA) ELISA Kit

Reactivity

Human

Applications

ELISA

Application notes

Human α2-Smooth muscle actin (α2-SMA) ELISA Kit employs a two-site sandwich ELISA to quantitate α2-SMA in samples. An antibody specific for α2-SMA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any α2-SMA present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human α2-SMA detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of α2-SMA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection method

Colorimetric

Sample type

Cell culture supernatants, Other biological fluids, Plasma, Serum

Assay type

Sandwich ELISA

Fig. Abbkine ELISA kit is series of ELISA to quantitate specific protein in samples.

Product Properties

Calibration range

5 ng/mL-80 ng/mL

Limit of detection

0.5 ng/mL

Usage notes

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage instructions

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping

Gel pack with blue ice.

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background

Actin alpha 2, the human aortic smooth muscle actin gene, is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, Moyamoya disease, and multisystemic smooth muscle dysfunction syndrome.

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact service@abbkine.com for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.