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Recombinant Human CCL22

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Product

Source

Size

Cat. No.

Price

Recombinant Human CCL22

E. coli

20 µg

Z101015

$70.00

100 µg

Z101017

$245.00

1.0 mg

Z101019

$1,350.00

Cat. No.

Product Name

Qty.

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CCL22 (monocyte-derived chemokine) was described initially as a constitutively produced, thymus-specific chemokine, implicated in the recruitment of T cells . One of the functions of CCL22 originally described was induction of migration of CCR4+ Th2 cells, but it has additionally been shown to regulate migration of Tregs. CCL22 has been implicated in a number of diseases, including allergen-induced lung inflammation, atopic dermatitis, and lymphoma. Similarly, a presumed role for CCR4+ cells has been suggested for endotoxic shock, rheumatoid arthritis, T cell lymphoma, and autoimmune diabetes

Gene Symbol

CCL22

Gene ID

6367

Accession No.

O00626

Source

E. coli

Appearance

Lyophilized Powder

Molecular Weight

8.0 kDa

Endotoxin Level

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Purity

>95% as determined by SDS-PAGE

Gel Image

Bioactivity

The ED(50) was determined by the dose-dependent proliferation of MGC823 cells and was found to be <5ng/mL.

Bioactivity Data

Formulation

Lyophilized from 0.2 μm filtered solution in Tris and NaCl, pH 7.0.

Reconstitution

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Storage

Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months.
Avoid repeated freeze/thaw cycles.

1. For estimating the endotoxin levels; we use the LAL (Limulus Amebocyte Lysate) method: The lysate from horseshoe crab amebocytes clots in the presence of very low endotoxin. This reaction is the basis of the Limulus amebocyte lysate (LAL) assay which was approved by the FDA in 1970.

· At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.

With regard to the BSA levels in some Growth Factors and Cytokines, can you please provide an explanation as to why they are so high?

The amount of BSA, as part of the formulation of a protein, can vary considerably depending on how much BSA was deemed optimum/necessary for protein stability in combination with /in-lieu of - other possible additives. The aforementioned formulations are somewhat analogous to the “carrier” versions of many formulations from “R and D systems” that have as high as 50 µg of BSA per µg of the recombinant protein product. If, needed or desired, abm scientists can substitute BSA for other stabilizing additives for most formulations.

Yes, all of abm's growth factors made in Escherichia coli using recombinant technology contain no human derived-products or other recombinant proteins. In the rare cases of BSA presence, this will be mentioned in the product's formulation.