Hi,
I am looking for protocols to stain larval imaginal discs
expressing nuclear Beta-Galactosidase (lacZ) reporter, either by
antibody staining or by activity staining.
I have done this in the past using reporters which were
strong expressors, however I have some transgenes that drive weak
expression, and I have not been able to visualize them succesfully.
If you have any protocol for this, I would really appreciate
if you share it with me, specially if you have any suggestions about
particular reagents, fixatives or buffers that seem to be critical in
your hands.
I appreciate your help.
Best,
--
Jesus Torres Vazquez
jtorresv at uci.edu
University of California, Irvine
4150 Bio. Sci. II
Irvine, CA 92697-2275
Dr. Kavita Arora's Lab
(949) 824-1223
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