Lentiviral Packaging Systems

NOTE: The Lenti-X HTX Packaging System (Cat. # 631247 and 631249) are being phased out. However, you can continue to purchase the same technology and obtain the same high titer results with Lenti-X Packaging Single Shots (VSV-g).

Lenti-X high-titer lentiviral packaging systems generate lentiviral titers that are superior to most other commercially available lentiviral packaging systems. At titers of 107-108 infectious units (IFU) per ml, you can often transduce an entire dish of target cells using only 10–100 µl of unconcentrated lentiviral packaging cell supernatant.

No need to concentrate virus

No need to transfect multiple plates

No need to infect your target cells multiple times

Easily control the expression level of your gene of interest by manipulating the multiplicity of infection (MOI)

Tetracycline transactivation—Very strong expression of several key lentiviral packaging components is produced by Tet-Off transactivation of tetracycline-responsive promoter elements (TREs). In many cell lines, including HEK 293 cells, the tetracycline transactivator generates absolute expression levels much higher than those produced by the CMV promoter.

Optimized transfections—Our exceptional, nanoparticle-based transfection reagent, Xfect, is included with every lentiviral packaging system. This reagent consistently transfects Lenti-X 293T cells with very high efficiencies (> 95%) and allows high expression of viral components.

The concerted effects of these advanced components allow Lenti-X 293T Cells (sold separately) to produce the highest amounts of safe, replication-incompetent lentivirus from any of our lentiviral vectors, or any other lentiviral vector.

The viruses are perfect for transducing delicate, hard-to-transfect cell types, such as primary neuroprogenitor cells. The standard Lenti-X HTX Packaging System is supplied with many of our lentiviral expression systems. Visit our lentiviral vectors page to see the variety of vectors and systems available.

Features

NOTE: The Lenti-X HTX Packaging System (Cat. # 631247 and 631249) are being phased out. However, you can continue to purchase the same technology and obtain the same high titer results with Lenti-X Packaging Single Shots (VSV-g).

Applications

Lentiviral transduction of a broad range of hard-to-transfect cell types

Easily create stable cell lines

Create ecoptropic lentivirus

Create VSV-G pseudotyped lentivirus

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.