[Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office (2007). The entire text is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm]

Age

newborn

Gender

Male

Applications

Irradiated cells for use as feeder layer. These cells are provided to be used as feeder cells to support the growth of stem cells in the undifferentiated state.

Storage Conditions

liquid nitrogen vapor phase

Clinical Data

Male

Comments

Once the feeder cells have attached, the culture medium can be changed to accommodate the cells to be supported. It is recommended that the feeder cells be plated 24 hours before use at 5 to 6 X 106/T75 in order to obtain a 100% confluent monolayer for stem cells growth.

These cells have been growth-arrested by irradiation with 6,000 rads. The cells will begin to deteriorate 2 weeks after plating and may no longer support the growth of other cells. We recommend that you do not keep the cells in culture for longer than 2 weeks.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 15%

This medium is formulated for use with a 5% CO2 in air atmosphere. (Standard DMEM formulations contain 3.7 g/L sodium bicarbonate and a 10% CO2 in air atmosphere is then recommended).

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.