Habitat: Lysterfield Park consists of approximately one third remnant vegetation and two thirds plantation. Within these areas about 300 plant species have been recorded. Some of the overstorey species native to the area include Yellow Box, Narrow-leaved Peppermint, Stringybark, Manna Gum, River Red Gum, Swamp Gum and Long-leaved Box.

In 1997 these Churchill National Park and Lysterfield Park were joined by the acquisition of more land and are now treated as a single park by Parks Victoria.

Lysterfield Park provides a diverse range of habitats and as such is a valuable wildlife refuge. The list of bird species recorded is about 140. Of these, 37 are waterbirds. Amongst the fauna, Eastern Grey Kangaroos and Black Wallabies are readily seen whilst Koalas, Antechinus and Echidnas are much harder to find. At night the park comes alive with Brushtail and Ringtail Possums, Sugar Gliders and various species of bats and owls.