Results: Figure 1 (A and B) shows representative 500₋MHz spectra from hippocampal regions of animals in binge₋ethanol and control groups. Figure 2 shows total creatine ratio levels that were quantified from 20 hippocampal tissue samples. Glutamate/tCr (**: p=0.007) and Glx/tCr (Glx: glutamine and glutamate complex [glutamate+glutamine]) (**: p=0.006) ratios were significantly higher in binge₋ethanol group than in control group.

Conclusion: Our findings suggest that glutamate signals and glutamate₋glutamine cycle in hippocampal region are particularly sensitive to acute₋binge ethanol consumption. Future studies using a combination of human patients and in vivo animal MRS investigations, as well as other neuroimaging approaches, are required to strengthen our findings and to validate translational component in acute₋binge alcohol intoxicated condition.