In addition to MALDI, several new ionization techniques, including desorption electrospray ionization (72), direct analysis in real time (73), and ESI-assisted laser desorption ionization (74), hold great potential for use in the real-time direct MS-based analyses of pathogens.

Chromatography and MS detection were performed using an Agilent 1100 solvent delivery system and a Waters Quattro Micro tandem mass spectrometer equipped with a Z-spray ion source operated in the positive electrospray ionization (ESI) mode.

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