Human Saa2 Serum Amyloid A2 Elisa Kit - China

This kit recognizes natural and recombinant Human SAA2. No significant cross- reactivity or interference between Human SAA2 and analogues was observed. Note： Limited by existing techniques, cross- reaction may still exist, as it is impossible for us to complete the cross- reactivity detection between Human SAA2 and all the analogues.

Repeatability

Coefficient of variation were<10% .

Test principle

This ELISA kit uses Sandwich- ELISA as the method. The micro ELISA plate provided in this kit has been pre- coated with an antibody specific to Human SAA2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human SAA2 and Avidin- Horseradish Peroxidase ( HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SAA2, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme- substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density ( OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SAA2. You can calculate the concentration of Human SAA2 in the samples by comparing the OD of the samples to the standard curve.