DNA Amplicon Sequencing

Targeted DNA sequencing with enrichment panels is a powerful approach to detect low-frequency variants. The main challenge with many of today’s
targeted DNA sequencing approaches is the generation of errors during
amplification steps. Because all DNA fragments look exactly the same, it
is impossible to distinguish a unique DNA variant from a library
construction error. Errors associated with amplification and sequencing
limit the ability of a researcher to confidently call low-frequency DNA
variants. ...

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Targeted DNA sequencing with enrichment panels is a powerful approach to detect low-frequency variants. The main challenge with many of today’s
targeted DNA sequencing approaches is the generation of errors during
amplification steps. Because all DNA fragments look exactly the same, it
is impossible to distinguish a unique DNA variant from a library
construction error. Errors associated with amplification and sequencing
limit the ability of a researcher to confidently call low-frequency DNA
variants.

The QIAseq Targeted DNA Panels have been developed to detect low-frequency variants with high confidence by overcoming the issues of PCR and sequencing error. This solution is based on the use of unique molecular indices (UMIs) to deliver a digital sequencing approach. Since each unique DNA molecule is barcoded before any amplification takes place, unique DNA molecules can be distinguished from PCR duplicates or errors − opening the door for you to confidently detect low-frequency DNA variants with new levels of statistical accuracy.