MLL1 Complex Chemiluminescent Assay Kit

Catalog #: 53008

Size: 96 reactions

$715

Description:

The MLL1 Complex Direct Activity Chemiluminescent Assay kit is designed to measure
MLL1 activity for screening and profiling applications, using purified MLL1 and its
complex components: WDR5, RbBP5,Ash2, and DPY30. The key to the MLL1
Complex Chemiluminescent Assay Kit is a highly specific antibody that recognizes
methylated K4 residue of Histone H3. With this kit, only three simple steps on a
microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine
is incubated with a sample containing assay buffer and methyltransferase enzyme for one
hour. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled
secondary antibody followed by the addition of the HRP substrate to produce
chemiluminescence that can be measured using a chemiluminescence reader.