Methanol Fixation

We usually use a warming plate (actually a slide dryer) that is set at about
65 C. However for our positive blood culture smears we use a 1 minute
methanol fix before GS.
A couple of weeks ago we had a case of Haemophilus in a blood culture and it
was almost impossible to find any organisms in the smear - with or without
the methanol fix.
--
John Gentile Rhode Island Apple Group
yjgent at home.com President
"I never make mistakes, I only have unexpected learning opportunities"
> From: "Joyce & Tim" <glenvale at home.com>
> Organization: Excite at Home - The Leader in Broadband http://home.com/faster> Newsgroups: bionet.microbiology
> Date: Thu, 19 Jul 2001 05:36:51 GMT
> Subject: Methanol Fixation
>> Does anyone have any information or a procedure for methanol fixation in
> bacterial smear preparation. I understand this technique produces less
> damage during prep since heating with a burner or incinerator is not
> required.
>> T. Burger
> Dept. of Biological Sciences
> Scott Community College
> 500 Belmont Road
> Bettendorf, IA 52722
>>tburger at eiccd.cc.ia.us>>