RE: [Histonet] negative immuno histochemistry control

From:

"Edwards, R.E."

For polyclonal antibodies use species specific(rabbit,goatetcetc) normal serum, or pre-immune if available: for mouse monoclonals use the specific isotypic e.g. IgG2a; simply omitting the primary antibody or replacing it with diluent alone is no control.
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of cynthia
haynes
Sent: 08 March 2006 12:50
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] negative immunohistochemistry control
Good Morning everyone, I have a question about immuno
staining. I've been away from this type of staining
for a while and now I am doing them again on a regular
basis. Why do you run a negative control with each
run? Are you only suppose to put the normal serum on
negative control only? I've forgotten; would someone
please answer these questions for me. Thanks in
advance.
Cynthia Haynes H.T.
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