Mucin isolated from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification) Human.

General notes

This antibody reacts with peptide core of gastric mucin restricted to the surface gastric epithelium. It characterised the M1-b epitope, completely destroyed by b-mercapto-ethano. The M1-b epitope is found located on a 58 kD and 40 kD peptide after trypsin treatment of native M1 mucin. This M1-b epitope coprecipites with the M1-f epitope encoded by Mucin 5AC gene. It is probably associated with the gastric mucin encoded by Mucin 5AC gene or with a gastric mucin encoded by another gastric MUC gene which polymerized with the M1/Mucin 5AC gastric mucin.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

Storage buffer

Preservative: 0.05% Sodium azideConstituent: PBS

Concentration information loading...

Purity

Protein G purified

Primary antibody notes

This antibody reacts with peptide core of gastric mucin restricted to the surface gastric epithelium. It characterised the M1-b epitope, completely destroyed by b-mercapto-ethano. The M1-b epitope is found located on a 58 kD and 40 kD peptide after trypsin treatment of native M1 mucin. This M1-b epitope coprecipites with the M1-f epitope encoded by Mucin 5AC gene. It is probably associated with the gastric mucin encoded by Mucin 5AC gene or with a gastric mucin encoded by another gastric MUC gene which polymerized with the M1/Mucin 5AC gastric mucin.

Target

Relevance

Mucins are high molecular weight glycoproteins with 80% carbohydrates and 20% core protein. Gastric Mucin 5AC antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden Mucin 5AC antigen.

Images

ab24071 at 1/1000 dilution staining MUcin 5Ac in Human bronchial epithelia by Immunocytochemistry. Well-differentiated HBE were exposed for 24 hr to mucosal PBS or mucosal SMM (Supernatant of mucopurulent material) before experiment. An immunoperoxidase detection system was used for staining.

Flow Cytometry - Anti-Mucin 5AC antibody [2-11M1] (ab24071)

Overlay histogram showing A549 cells stained with ab24071 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24071, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

While we are unaware of any references showing this, both ab24071 and ab24072 will be guaranteed to react with cow Mucin 5AC in IHC-Fr. These antibodies are very similar and should give similar results, the only d...

Thanks for your reply.
Are these are human tracheal epithelial cells? In addition to the 1:100 antibody dilution, you should also stain your membrane with Ponceau dye to make sure that the protein transferred successfully.
I look forward ...

My colleague Heather is out of the office but I will be happy to help you further with this.
I have a couple of additional questions as well. What kind of cells are you studying? Is the supernatant processed before being loaded into the gel?
...

I am very sorry to hear the pretreatment did not resolve the problem. I understand your position and I regret not to be able to give you more specific information, I have arranged a refund on your order.
Best of luck with your experiments. I hope t...

I was informed today that pretreatment with periodate solution (0.1 - 0.05M) is recommended for 10-20 minutes when detectin mucin in IHC.
There are 2 publications which describe the periodate method:
Int.J. Cancer 47; 304-310 (1991)
J.Immunol.M...