Abstract: Satureja abyssinica (Benth.) Briq. is an endangered medical and aromatic herb that belongs to Lamiaceae (Labiateae) family and well known for its antimicrobial and antifungal properties. This species is highly threatened due to continuously collecting from the wild and absence of germplasm conservation techniques. In this study, direct organogenesis protocol has been developed for this species through nodal segment explants. MS medium containing different concentrations of 6-benzyl aminopurine (BAP), Kinetin (KIN), α-Naphthalene acetic acid (NAA), Indole-3- butyric acid (IBA) and indole acetic acid (IAA) were utilized in this experiment. Best culture establishment (90%), maximum average of shoot (3.30±0.47) and shoot length (2.35±0.28 cm) were observed on initiation medium containing 1.0 mg/l BAP. Multiple shoots with highest (3.90±0.60) of shoots per nodal segment and shoot length (1.10±0.16 cm) were obtained at 1.0 mg/l BAP. Maximum root number (8.00±1.12) and 90% rooting frequency were achieved on half-strength MS medium supplemented with 0.5 mg/l IBA. Mean of longest root (1.07±0.09 cm) was produced in the presence of 1.4 mg/l IBA. After acclimatization, 88% plant survived. This protocol can be used for rapid propagation and consequent improvement and conservation of S. abyssinica.