Egg parasitoids of the genus Trichogramma (Hymenoptera: Trichogrammatidae) are important natural enemies of many lepidopterous
pests in agricultural and forest areas. Because the use of indigenous Trichogramma populations/species can significantly increase
the biological control efficiency, the characterization of endemic populations and precise species identification is important.
In this study, Trichogramma wasps were collected from parasitized eggs of several lepidopteran pests in Northern and Central
Iran. Seven Trichogramma species were identified based on the morphology and the nucleotide sequence, size and restriction
profile (PCR-RFLP) of the internal transcribed spacer 2 (ITS2) region of the rDNA of T. brassicae Bezdenko, T. cacoeciae Marchal,
T. embryophagum Hartig, T. evanescens Westwood, T. euproctidis Girault, T. pintoi Voegelé and T. tshumakovae Sorokina. This
is the first report of T. euproctidis in Iran. Additionally, natural populations were surveyed for the prevalence of Wolbachia.
Two populations of T. brassicae were found to be infected by a single Wolbachia strain which was identified by using a multi-locus
sequencing approach.

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