As the leading
manufacturer and supplier of poly lysine coated German coverslips,
neuVitro has developed a 5-day procedure with our state of the art
facility to generate the best professional coating on German coverslips
for challenged cell cultures. All coated coverslips are sterilized by
radiation and ready to use immediately.

Questions
and Answers:

How much poly
lysine needed to coat my coverslips?

The required
amount of poly lysine is 7.5ug/cm² surface area of your coverslip.
For example, to coat 100 pieces of 18mm diameter coverslips, the total
surface area of 100 pieces of coverslips is 0.9x0.9x3.1416 x2x100 =
510cm². The required poly lysine is 510x7.5ug = 3825ug = 3.825mg.

Should I coat
coverslips in dd-water or buffer?

Poly lysine
coating in dd-water is simple. But the pH of dd-water is acidic,
usually lower than pH6, which is not optimized for poly lysine
coating. Higher pH value, such as pH 8.5 in borate buffer, enhances
poly lysine coating. But the buffer residue must be removed thoroughly
from coverslips before cell culture.