[1]佘晨兴,王静,苏玉萍,等.三十六脚湖水库沉积物聚磷菌多样性及群落组成[J].应用与环境生物学报,2018,24(06):1254-1262.[doi:10.19675/j.cnki.1006-687x.2018.03001] SHE Chenxing,et al..Diversity and community composition of phosphorus accumulating organisms (PAOs) in the sediments of the Sanshiliujiao Lake reservoir[J].Chinese Journal of Applied & Environmental Biology,2018,24(06):1254-1262.[doi:10.19675/j.cnki.1006-687x.2018.03001]

The diversity and community composition of phosphorus accumulating organisms (PAOs) in the sediments of the Sanshiliujiao Lake reservoir in Pingtan island, Fujian Province, were investigated by terminal restriction fragment length polymorphism (T-RFLP) methods combined with the construction of clone libraries. The diversity of PAOs in summer samples was significantly higher than that in winter. The dominant terminal restriction fragments (T-RFs) of PAOs were in the range of 200 to 300 bp in summer, while they were in the range of 150 to 200 bp in winter. Cloning sequencing and phylogenetic analysis showed that the main phyla of PAOs in the sediment of the Sanshiliujiao Lake reservoir were Proteobacteria, Actinobacteria, and Acidobacteria. Anaeromyxobacter, Azospirillum, Microlunatus phosphovorus, and Burkholderia were the dominant genera in the summer samples. Anaeromyxobacter, Methylobacterium, Solibacter, Azospirillum, and Microlunatus phosphovorus were the dominant genera in sediment samples in winter. The results of this study demonstrated that the diversity and community composition of PAOs in the sediments of the Sanshiliujiao Lake reservoir had specific seasonal characteristics. Various forms of phosphorus also showed their influence on the diversity of PAOs, especially Fe/Al-P. Our findings lays a scientific foundation for the future disclosure of microbial mechanisms involved in the phosphorus metabolic cycle in the sediments of reservoirs.