We previously proposed the existence of DNA to DNA transcription ineukaryotic cells, but the mechanism by which single-stranded DNA ssDNAtranscript is produced and released from the genome remains unknown. We oncespeculated that the mechanism of DNA to DNA transcription might be similar tothat of DNA to RNA transcription, but now we propose that endonuclease dependenttranscript cutout may be a possible mechanism of DNA to DNA transcription, inwhich a copy of ssDNA fragment transcript between two nicks produced by nickingendonuclease is released from double-stranded DNA dsDNA region in the genomeby an unknown ssDNA fragment releasing enzyme. The gap in the dsDNA will befilled through DNA repair mechanism. Occasionally, multiple copies of ssDNAtranscripts could be produced through multiple rounds of cutout-repair-cutoutcycle.