Fig. 4

Influence of r-tPA on oxidative burst. Oxidative burst analysis was performed using blood from healthy donors for granulocytes (a) and monocytes (b). Blood samples of healthy donors were incubated with 0.5 or 1 μg/ml r-tPA for 4 h at 37 °C in 5% CO2. The oxidative burst was then induced using fMLP or PMA, and the efficacy (defined as the mean fluorescence intensity, MFI) of ROS was measured by flow cytometry (n = 10). *p < 0.05; **p < 0.01; ***p < 0.005. The mean and SD ranges are given. Repeated measures ANOVA and Bonferroni comparisons as post hoc tests were used within each of the four stimulation conditions