Restriction Digestion of Plasmid, Cosmid, and Phage DNAs
(Donis Keller Lab)This is a generalized example of a restriction digest. Estimate the amount of DNA needed in your digest and scale up accordingly. To visualize a digest on an ethidium bromide-stained agarose gel, you will need to take the size of the fragments and the total size of the clone DNA into account (e. g. 10-50 ng of intact lambda-sized genomes (~50 kb) are easily seen on gels but if cut into small (~1kb fragments), the relative proportion of the clone DNA in each fragment is ~ 1/50 and more DNA (500-1000 ng) should be loaded in order to see them).http://hg.wustl.edu/hdk_lab_manual/rdm/rdm3.htmlAdded: Tue May 14 2002, Hits: 407, Reviews: 0Write reviewCached