MGI assigns GO annotation by either manual curation of published experimental literature or automated electronic-based methods. The annotated records include the supporting references. MGI collaborates with several groups outside of MGI in collecting GO annotation for mouse genes. See GO Curators for Mouse for the list of groups that provide annotations for mouse genes.

Electronic-based annotation strategies include:

mapping the Enzyme Commission (EC) numbers associated with a gene to the EC number attached to GO terms (referenced with J:72245)