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When asking make sure to include the operating system and the revision of Pandoras Boa you are using. If your question is related to hardware, please state if you are using coolux hardware (which one) or if you built your own.

I'm working with the last PB v6 software, edu.I would like to make a mapping on a video tile 16 X 320 pixels:My both screens (outputs) are 1920 X 1080 pixels, so this is my GUI en the signal for the LED processor.

When I make the map in the matrix patcher I select a patchsize of 1920 X 1080, and then i place the screen on the correct position. Initialise map, and scale to the source content I would like to make visable.I export the Map to a layer with remap FX on it. When I playback a clip with the resolution of 1920 x 1080 everything works perfect, so far so good.

But, when I put a clip with a different resolution to this layer, the pixel map changes!The project I am doing needs a lot of different content sizes on the spot.Is there a way this mapping doesn't change when I change the content size?

No I didn't becouse this would not work for me.I have 5 screens with several configurations (single screen on single layer and single layer for all screen toghether etc.), so I use more then one matrix patcher map........(each layer a different map)

there is no button or checkbox to change that.The Matrix patcher always works with the content resolution.

There are some "cheats" to get a different resolution.You can make a warp file to go to the 1920x1080 and scale the content down with the UV Resize to the normal one.Or make different remaps.Or make warp files instead of remaps.

The Remaps only work on a per-pixel base if both the media and the outgoing pixel sizes are the same as the one that you specified in the patch. Everything else would not be pixel-perfect. Since the remap will only remaps single pixels and not rectangles it is only useful to remap same resolutions. There are some tricks you can do to map different resolutions that mostly involve the warper and the Layer Inspector.

You have to provide more information on resolutions and what you want to map.

As Benni said, you can also use the warper or V6 warping to remap stuff.