Supplementary Material

Gating strategy for Treg. Flow cytometry dot plots and pseudodot plot are shown. Cells gated on CD4+ lymphocytes are then gated on CD25+ and FoxP3+ to quantify Treg. CD45RA+ and CD45RA- populations were quantified among Treg. Treg expressing the highest levels of CD25 include the CD45RA- cells while CD45RA+ Treg express intermediate levels of CD25.