This study was performed to evaluate the effect of Myrtle essential oil (MEO) on intestinal morphology, antibody titres and blood parameters of broiler chicks. A total of 180 Ross 308 broiler chickens were allocated to 5 dietary treatments with 3 replicates of 12 birds each. The experimental diets consisted of diet free of antibiotics (control diet), supplemented diets with MEO at levels of 100, 300 and 500 mg/kg, as well as flavomycin antibiotic at 450 mg / kg. The results showed that supplementation of MEO and antibiotic compared to the control group significantly prolonged the height of the villus height and lower the epithelial thickness of the small intestine at the age of 42 days (p<0.05). There was no significant difference between different treatments in relation to the width of the villus and the villus height to crypt depth ratio. The highest antibody titer against influenza and Newcastle disease was observed in chicks fed 500 mg/kg MEO (p<0.05). The effect of different levels of essential oil on hepatic enzymes including AST, ALT and ALP was not significant (p> 0.05). Overall, the results show that the supplementation of MEO diets in improved intestinal morphology and increased antibody titers, especially at a level of 500 mg / kg, which could be considered as an antibiotic alternative.