1.E.20.1.4Holin of 115 aas and 3 TMSs, pyocin R2 holin. Functions in DNA release within biofilms (Wang et al. 2016). A gene cluster encoding a bacteriophage-derived pyocin and its lysis
cassette was upregulated in phenozine producing strains. A holin encoded in this gene cluster was found to contribute to the release of
eDNA in biofilm matrices, demonstrating that the influence of
the phenozine, 2-OH-PCA, on eDNA production is due in part to cell autolysis as a result
of pyocin production and release (Wang et al. 2016).