Welcome to the Winobloggers webpage! Students and professors from different universities meet to discuss their findings on Winogradsky columns they constructed!
We learn how to formulate hypotheses, observe, comment, ask and reply to questions related to our scientific experiments.

Winogradksy columns

'In the field of observation, chance only favors the prepared mind' Pasteur 1854

Blog posts

Friday, 4 November 2016

Team:
4

Tampakidou Maria-Ioanna, 1479

Mpelonias Fivos, 1178

Introduction

The experiment was constructed at 21/10/2016 in the
lake Vrellis, which is a small, seasonal, shallow lake 12 km outside of
ioannina city. The goal of this experiment is the construction of a winograsky
column. Actually two winograsky columns were constructed in order to involve two different factors
whom any discrapancies could be observed.

Materials-Method

·2 plastic bottles (1,5 L)

·Mud sediment from the lake

·Lake water

·2 eggs

·Piece of newspaper

·Plaster

·Plastic membrane

These columns contains muddy sediment
whish was taken from the lake and was mixed with nutricius materials that are
full of carbon, soulphate and protein sources. In the first case, mud has been
added and mixed with two eggs (protein and soulphate source - buffer factor)
and pieces of newspaper (carbon source) in a plastic bottle. In the second
case, instead of eggs, has been added plaster (full of sulfur). After the bottled
have been half contained with the
nutricious mud, extra mud without nutrients was added so the 2/3 of the bottles
were full. Aferwards, water from the
lake was added up immediately. Moreover,
it should be indicated that the second plastic bottle has been cut and coating
with plastic membrane

Muds sediment reposit.

Hypothesis

At first, the basic hypothesis is the growth of
several types of microorganisms such as different kinds of bacteria and
phytoplangton . We consider as a contributory factor of the microbes
increasement, the fact that the lake
ranging from mesotrophic to eutrophic.
The diversity of microorganisms will be depended on the gradation of the
oxygen across the bottle and the presence of soulphates. Therefore, in the top
of it will be grown aerobic microorganism such us photosynthetic cyanobacteria
and algae, in the middle of the bottle will be grown aerotolerant microbes that they do not use sulful (there
is not any of it in this level) such us non-sulfur photosynthetic bacteria
(anaerobic zone: less anaerobic to more anaerobic). At the bottom of the
bottle, the presence of totally anaerobic photoautotrophic microbes propably
will be observed. These microbes use sulfur ions to produce energy. Microorganisms
like the phylum clorobi convert sulphates ions into hydrogen sulfide which
afterwards could be used by other anoxic tolerant organisms.

There is one more conjecture in this experiment which
is relevant with the difference between the nutrient conditions in the two
bottles. More specific, in the first bottle the increase of various
microorganisms will be bigger than the second because eggs contains a variety
of nutrients like proteins, fatty acids,
minerals.