Mount Sinai Scientists Take Novel Approach to Restore the Microbiome of C-section Newborns in an Effort to Foster Improved Health

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Scientists from the Icahn Institute for Genomics and Multiscale Biology at the Icahn School of Medicine at Mount Sinai, collaborating with NYU Langone Medical Center and a multi-center team of researchers, demonstrated for the first time that the microbiome of newborn babies delivered via cesarean section (C-section) can be partially restored to resemble that of vaginally delivered infants.

New York, NY (PRWEB)February 01, 2016

Scientists from the Icahn Institute for Genomics and Multiscale Biology at the Icahn School of Medicine at Mount Sinai, collaborating with NYU Langone Medical Center and a multi-center team of researchers, demonstrated for the first time that the microbiome of newborn babies delivered via cesarean section (C-section) can be partially restored to resemble that of vaginally delivered infants. By swabbing newborns with a gauze containing vaginal fluids from their mothers, and later testing samples collected from these newborns and their mothers using state-of-the-art genomic technology and computational methods, the research team showed that C-section infants became enriched in vaginal bacteria such as Lactobacillus, which were nearly absent in untreated infants. The team published its findings today in the journal Nature Medicine.

C-section rates have increased significantly worldwide over the last few decades. More than a third of all births in the U.S. are now by C-section, well above the 10 to 15% recommended by the World Health Organization. Although often a life-saving procedure for mothers and infants, this procedure comes with undesirable side effects for the newborn, including an increased risk for immune and metabolic conditions. This risk has been attributed to the aberrant microbiome found in newborns delivered via C-section, resulting from their lack of exposure to maternal vaginal microbes. The human microbiome encompasses the trillions of bacteria harbored in the body that are essential to our well-being.

“There is a clear association between C-section and increased risk for several diseases,” said senior author of the study Jose C. Clemente, PhD, Assistant Professor of Genetics and Genomics and a member of the Icahn Institute at the Icahn School of Medicine at Mount Sinai, “but to date, only a few research studies in mice have demonstrated that this is caused by difference in the microbiome early in life. Our work is the first to demonstrate in humans that we can modify the abnormal bacterial communities found in C-section babies.”

The pilot study recruited seven mothers expected to deliver vaginally and 11 by scheduled C-section, out of which four agreed to have their babies swabbed upon birth. A sterile gauze was incubated in the maternal vagina during the hour previous to the C-section, and the babies were swabbed on their mouth, face, and body immediately after delivery.

Over 1,500 samples were collected at multiple time points from several body sites of the infants and their mothers during the first month of life. The research team harnessed recent advances in DNA sequencing and big data analytics to characterize, for the first time, the microbial communities found in the infants delivered vaginally and by C-section. Analysis of these results determined that C-section infants exposed to their maternal vaginal fluids had a microbiome enriched in bacteria commonly found in vaginally delivered babies, such as Lactobacillus and Bacteroides. These bacteria, nearly absent in C-section children that were not swabbed, have been linked to a proper development of the immune system, and could provide an explanation for the lowered disease risks associated with vaginal delivery.

“This study has allowed us to demonstrate the feasibility of bacterial restoration in a small cohort, but we do not know yet whether this procedure alone is sufficient to restore the health benefits associated with vaginal delivery”, said Dr. Clemente. “Studies that target children at risk for immune disorders, such as allergies, will be required to determine how microbial restoration affects health outcomes”.

This work was partially supported by a grant from the Crohn’s and Colitis Foundation of America and the SUCCESS grant to Dr. Clemente. Support was also provided by the C&D Research Fund, and National Institutes of Health grant R01 DK090989. Computing was supported by the Department of Scientific Computing at the Icahn School of Medicine. Computational analytics was supported by the Icahn Institute for Genomics and Multiscale Biology at the Icahn School of Medicine.

About the Mount Sinai Health System
The Mount Sinai Health System is an integrated health system committed to providing distinguished care, conducting transformative research, and advancing biomedical education. Structured around seven hospital campuses and a single medical school, the Health System has an extensive ambulatory network and a range of inpatient and outpatient services — from community-based facilities to tertiary and quaternary care.

The System includes approximately 6,100 primary and specialty care physicians; 12 minority-owned free-standing ambulatory surgery centers; more than 140 ambulatory practices throughout the five boroughs of New York City, Westchester, Long Island, and Florida; and 31 affiliated community health centers. Physicians are affiliated with the renowned Icahn School of Medicine at Mount Sinai, which is ranked among the highest in the nation in National Institutes of Health funding per investigator. The Mount Sinai Hospital is ranked as one of the nation’s top 10 hospitals in Geriatrics, Cardiology/Heart Surgery, and Gastroenterology, and is in the top 25 in five other specialties in the 2015-2016 “Best Hospitals” issue of U.S. News & World Report. Mount Sinai’s Kravis Children’s Hospital also is ranked in seven out of ten pediatric specialties by U.S. News & World Report. The New York Eye and Ear Infirmary of Mount Sinai is ranked 11th nationally for Ophthalmology, while Mount Sinai Beth Israel is ranked regionally.