GeneRead DNAseq Mix-n-Match Panels V2

For targeted enrichment of a customizable gene panel using any of 570 laboratory-verified primer sets prior to NGS

Outstanding sequencing performance

As little as 40 ng DNA needed

Compatible with many samples types including FFPE samples

Can be used on any sequencing platform

Customizable using any of 570 pre-verified gene primer sets

GeneRead DNAseq Mix-n-Match Panels V2 are the simplest tools for analyzing the genetic variants of a customized panel of genes via next-generation sequencing. Each panel consists of wet-bench verified multiplex PCR primer sets, sufficient for 96 samples, to amplify exonic regions of a thoroughly researched panel of biologically and clinically relevant and disease-focused genes. These panels are chosen by you to include the genes most relevant to your specific research interests. Our high-quality primer design and target enrichment chemistry provide high design coverage, specificity, and uniformity, which are essential for detecting low-frequency variants in your precious samples.

The principle of the GeneRead DNAseq V2 System is to employ overlapping primer sets across the exonic portions of a gene or genes to maximize target coverage and minimize nonspecific amplification.

Performance

The performance of GeneRead DNAseq Mix-n-Match Panels is assessed by three criteria: design coverage, specificity, and coverage uniformity. Greater than 90% of exonic regions are covered by the panel primer design, enhancing potential variant discovery. The high specificity of the system maximizes efficient use of sequencing capacity, as more than 90% of sequencing reads align to target regions. Finally, the high coverage uniformity of the system, with more than 90% of targeted bases covered at >20% median sequencing depth, ensures high-quality variant calls.

Principle

The GeneRead DNAseq Panels V2 System employs overlapping primer sets across the exonic portions of a gene or group of genes to maximize target coverage (see Multiplex PCR-enabled target enrichment of genomic regions of interest). Overlapping primer sets are divided into 4 pools, thereby increasing the specificity of coverage without primer dimers and nonspecific amplifications. Following amplification and purification, enriched regions from each sample are pooled together, yielding one library preparation for each sample.

Procedure

GeneRead DNAseq Mix-n-Match Panels are part of a total workflow for targeted next-generation sequencing (see figure, GeneRead DNAseq Panel V2 System workflow). Simply extract DNA from your samples (the QIAamp DNA Mini Kit, the QIAamp DNA FFPE Tissue Kit, or the GeneRead DNA FFPE Kit are are recommended), quantify and qualify your DNA sample with the GeneRead DNA QuantiMIZE System, and then use the GeneRead DNAseq Mix-n-Match Panels V2 in combination with the GeneRead DNAseq Panel PCR Kit to perform targeted enrichment using multiplex PCR. Once targets have been enriched, construct the NGS library and use the GeneRead DNAseq Library Quant Array to quantify and perform quality control. Then perform NGS and analyze your data using the QIAGEN NGS Data Analysis Web Portal.

The GeneRead DNAseq Custom Panel Builder is now available online at GeneGlobe. To build your custom panel, go to GeneRead DNAseq Custom Builder under Design tools in GeneGlobe.

Applications

GeneRead DNAseq Mix-n-Match Panels V2 are highly suited for enriching target genes specific for various cancers, inheritance diseases, neurological diseases, cardiomyopathy, development disorders, metabolic disorders, and many other areas of biomedical research to determine genetic variants through NGS.