FCS Measurements

FCS Data

The above image show an average correlation curve of 10,000x diluted green fluorescent beads

Calibration Curves

The above image is a calibration curve of 1/N vs. concentration for the Oligo-D samples. We observed a very linear relationship between these samples, with an R2 value of 0.99.

The above image is a calibration curve of 1/N vs. concentration for the DNA/MB samples. The 100pM and 75pM samples seem to follow the linear trend in relation to one another, however, the 50pM sample is a clear outlier. Because we did not see the pattern we are expecting for all three samples, we will prepare fresh solution tomorrow and run them again to see if there was some kind of error in sample preparation.