Pisani joined his father Niccolò during the third war with Genoa (1350–55) and later distinguished himself in a war against Hungary. Named captain and senator, he led a squadron against the Aragonese fleet and the pirates in the waters near Sicily.

In 1378 he was given command of the Venetian fleet, which he directed to victory over the Genoan fleet at the Capo d’Anzio (May 1378). He later held the Genoan fleet on the Adriatic in check, after sacking Cattaro (modern Kotor, Montenegro, Yugos.) and destroying the port of Sebenico (modern Šibenik, Croatia). Eventually, however, he and his fleet were captured by the enemy at Pola (modern Pula, Croatia; May 1379). On his return to Venice, he was imprisoned, but popular protest secured his release. Given command once again of the Venetian fleet, he blockaded the port of Chioggia, occupied by the Genoans, who were finally compelled to surrender, turning over to Pisani some 19 galleys and more than 4,000 prisoners. Dispatched to confront another Genoan squadron in the Adriatic, Pisani fell ill and died.