Pure & Clean Isolation Samples

Isolating exosomes with size exclusion chromatography (SEC)

Size Exclusion Chromatography (SEC) is the most effective method for isolating exosomes from cell culture supernatants and complex biological fluids. Since the separation is based on size, vesicles flow through the column un-retained and elute in the void volume. Proteins and other contaminants that are smaller than the pores of the stationary phase are retained by the column and elute later. Other methods that are non-specific in nature require overnight incubation of vesicles with precipitation buffer. As a result, vesicular and non-vesicular particles are isolated together so additional steps are needed to separate EVs from contaminating particles. In contrast, isolations using qEVsingle SEC columns take 15 minutes and remove > 99% of contaminating background proteins from your samples in a single isolation. SEC columns, manufactured to quality standards, provide the only separation method that can be standardized sufficiently for medical trials and subsequent clinical use.

Isolating exosomes with size exclusion chromatography (SEC)

Size Exclusion Chromatography (SEC) is the most effective method for isolating exosomes from cell culture supernatants and complex biological fluids. Since the separation is based on size, vesicles flow through the column un-retained and elute in the void volume. Proteins and other contaminants that are smaller than the pores of the stationary phase are retained by the column and elute later. Other methods that are non-specific in nature require overnight incubation of vesicles with precipitation buffer. As a result, vesicular and non-vesicular particles are isolated together so additional steps are needed to separate EVs from contaminating particles. In contrast, isolations using qEVsingle SEC columns take 15 minutes and remove > 99% of contaminating background proteins from your samples in a single isolation. SEC columns, manufactured to quality standards, provide the only separation method that can be standardized sufficiently for medical trials and subsequent clinical use.

Reproducible, Efficient, Fast.

qEVsingle columns are designed for single-use, removing the need for a regeneration step and ensuring the highest purity and consistency. They are gentle on vesicles. As opposed to ultracentrifugation and precipitation, SEC purified EVs retain their biological function and structure. SEC purified exosomes are free of contaminating proteins. Vesicle recovery from column-to- column is very reproducible. Since samples are isolated without extreme centrifugal force, there are no protein aggregates or vesicle aggregation. The small size and price makes these columns ideal for single use applications where sample cross contamination needs to be avoided, e.g. DNA, RNA or patient sample analysis. For repetitive, limited volume applications these smaller columns provide the ideal solution, resulting in reduced sample dilution for small samples during separation, compared with the larger standard qEV columns.