Diarrheagenic Escherichia coli (DEC) are often disseminated through the fecal matter of livestock and waste products including slurry and manure. The study aimed to characterize archived DEC recovered from cattle fecal matter, manure and slurry for quinolone resistance and extended spectrum beta-lactamases (ESBLs) with focus on trends in antimicrobial susceptibility patterns. The susceptibility of the bacteria was tested using standard laboratory procedures. Polymerase chain reaction (PCR) was carried out to detect the presence of qnrA, qnrB, qnrS genes and β-lactamase producing genes (blaESBL) such us blaTEM and blaSHV. About 91% of DEC strains were multidrug resistant (MDR) with non-susceptibility to ≥1 agent in ≥3 antimicrobial classes. The most common resistance was to amoxicillin-clavulanic acid (96.36%), followed by tretacycline (89.09%), ceftazidime (76.36%), and cefotaxime (70.780%). qnrS (18.2%) was the most prevalent quinolone resistant genes, followed by qnrB (7.2%) and qnrA (2%). blaTEM (5.45%) was most prevalent than blaSHV genes (3.6%). blaTEM and blaSHV genes were identified in double or multiple-carrying with qnrS and qnrB, no Beta-lactamase (ESBLs) producing strains were observed. This result highlights the importance of livestock fecal matter, manure, and slurries as a significant public health concern and a repository of antibiotic resistant gene.