Be
sure to check the full excitation and emission spectra of your fluorophores
- particularly with multiple labels. Many spectra are available on Molecular
Probes' web site at http://www.probes.com

The
457 line excites CFP.
The 488 line excites GFP, Alexa 488, Cy2, fluorescein,
and Propidium Iodide.
The 543 line excites the RFPs, Alexa
568 and 555, Cy3, Rhodamine, and Propidium
Iodide.
The 633 line excites Alexa 633 and 647, Cy5 and ToPro3