JavaScript is disabled for your browser. Some features of this site may not work without it.

Data from: Surviving in mountain climate refugia: new insights from the genetic diversity and structure of the relict shrub Myrtus nivellei (Myrtaceae) in the Sahara desert

Migliore J, Baumel A, Juin M, Fady B, Roig A, Duong N, Médail F

Date Published: September 27, 2013

DOI: http://dx.doi.org/10.5061/dryad.1vt02

Files in this package

Content in the Dryad
Digital Repository is offered "as is." By downloading files, you agree
to the Dryad Terms of Service.
To the extent possible under law, the authors have waived all copyright
and related or neighboring rights to this data.

Title

Migliore et al - AFLP Myrtus nivellei

Downloaded

36 times

Description

AFLP (Amplified fragment Length Polymorphism) dataset examined for 215 individuals of Myrtus nivellei (Myrtaceae) sampled within 23 wadis in the central Saharan mountains, and listed per row. Each individual is denoted by a four-letter code: one letter for the country (A for Algeria), two letters for the mountain range (TA for Tassili n’Ajjer, HO for Hoggar and IM for Immidir), and one letter for the wadi sampled, with a supplementary letter for individuals sampled within the Tarit wadi (AIMT) more intensively analyzed.Three combinations of primers have been selected: EcoRI-AAGG with MseI-CCAG, EcoRI-AAC with MseI-CAC, EcoRI-AAC with MseI-CAA. AFLP multilocus profiles were transcribed in a matrix summarizing the presence (1) or the absence (0) of AFLP markers (123 markers retained and presented in column).

SSR (simple sequence repeats) dataset examined for 215 individuals of Myrtus nivellei (Myrtaceae) sampled within 23 wadis in the central Saharan mountains, and listed per row. Each individual is denoted by a four-letter code: one letter for the country (A for Algeria), two letters for the mountain range (TA for Tassili n’Ajjer, HO for Hoggar and IM for Immidir), and one letter for the wadi sampled, with a supplementary letter for individuals sampled within the Tarit wadi (AIMT) more intensively analyzed. Eleven nuclear microsatellite markers (Myrcom1 to Myrcom11) developed for Myrtus communis by Albaladejo et al. 2010 (see reference [48] in the article) were used in this study; only the seven polymorphic loci were presented here.

AbstractThe identification of past glacial refugia has become a key topic for conservation under environmental change, since they contribute importantly to shaping current patterns of biodiversity. However, little attention has been paid so far to interglacial refugia despite their key role for the survival of relict species currently occurring in climate refugia. Here, we focus on the genetic consequences of range contraction on the relict populations of the evergreen shrub Myrtus nivellei, endemic in the Saharan mountains since at least the end of the last Green Sahara period, around 5.5 ka B.P. Multilocus genotypes (nuclear microsatellites and AFLP) were obtained from 215 individuals collected from 23 wadis (temporary rivers) in the three main mountain ranges in southern Algeria (the Hoggar, Tassili n’Ajjer and Tassili n’Immidir ranges). Identical genotypes were found in several plants growing far apart within the same wadis, a pattern taken as evidence of clonality. Multivariate analyses and Bayesian clustering revealed that genetic diversity was mainly structured among the mountain ranges, while low isolation by distance was observed within each mountain range. The range contraction induced by the last episode of aridification has likely increased the genetic isolation of the populations of M. nivellei, without greatly affecting the genetic diversity of the species as a whole. The pattern of genetic diversity observed here suggests that high connectivity may have prevailed during humid periods, which is consistent with recent paleoenvironmental reconstructions.