Corning® FluoroBlok™ Cell Culture Inserts

Corning® FluoroBlok™ Cell Culture Inserts

Patented Light-tight PET Membrane

FluoroBlok cell culture inserts are designed with a patented light-tight polyethylene terephthalate (PET) membrane that efficiently blocks the transmission of light between 400 and 700 nm, allowing fluorescence detection in a simplified and non-destructive manner. Fluorescently labeled cells present in the top chamber of the insert are shielded from bottom-reading fluorescence plate readers and microscopes by the FluoroBlok membrane.

After labeled cells migrate through the membrane, they are easily detected by a bottom-reading fluorescence plate reader or microscope, thereby eliminating manual cell counting and additional processing steps. This non-destructive detection method enables both kinetic and endpoint migration and invasion assays.

Key Benefits:

FluoroBlok permeable supports provide these key benefits:

Saves time by eliminating swabbing and manual cell counting for cell migration and invasion assays

Available in a wide array of formats and configurations from individual inserts to 96-well plates

Suitable for both pre- and post-fluorescently labeled cells

Enhances data content with the ability to take multiple readings from the same well during kinetic assays

*Values are reported as nominal and may vary due to inherent variability of our manufacturing process. To ensure success, we recommend that researchers validate their methods independent from our reported values.

*Values are reported as nominal and may vary due to inherent variability of our manufacturing process. To ensure success, we recommend that researchers validate their methods independent from our reported values.

FluoroBlok HTS and BioCoat™ Systems

FluoroBlok HTS and BioCoat™ Systems

Improve assay reproducibility with Corning Matrigel® matrix pre-coated FluoroBlok systems. These are available as the Corning BioCoat 24- and the 96-HTS multiwell insert systems. The systems also offer an automation-friendly format compatible with most robotics and fluid handlers, and they allow users to analyze 24- or 96-multiwell inserts simultaneously, because all insert wells are part of a sin­gle plate. In addition, Corning FluoroBlok multiwell insert systems provide reliable, lot-to-lot consistent protein coatings for tumor cell inva­sion and endothelial cell migration and invasion assays.

Optimized assay sys­tems are also available for in vitro angiogenesis and tumor cell inva­sion assays:

Corning BioCoat angiogenesis systems are composed of 3.0 μm inserts with biological coatings optimized for use in endothelial cell migration and invasion assays.