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Wednesday, October 22, 2014

Alexa Fluor® 680 is a far-red-emitting dye with peak excitation at 684 nm and peak emission at 702 nm. Alexa Fluor® 790
is an infrared-emitting dye with peak excitation at 783 nm and peak
emission at 803 nm (Figure 1). Antibodies conjugated with far-red- and
Infrared-emitting dyes are more sensitive than those with dyes emitting
visible light due to low fluorescence quenching of the conjugates, high
extinction coefficients of the dyes, and low background
autofluorescence. The increased brightness allows for a wider range of
immunofluorescence detection and imaging modalities. Far-red and
Infrared dye conjugates can be used for higher sensitivity Western
blots, quantitative Western blots, in-gel Western blots, microWestern
arrays, in-cell Western arrays, on-cell Western arrays, tissue section
imaging, small animal whole body imaging, and other techniques that
require the brightest dyes.

Jackson ImmunoResearch now offers the largest selection of Alexa
Fluor® 680 and Alexa Fluor® 790 dyes conjugated with signal-enhancing
primary antibodies, affinity-purified secondary antibodies,
streptavidin, and purified IgG controls for single and double-labelling
Western blots (Figure 2) and other tecniques requiring high
sensitivity. The secondary antibodies are adsorbed to eliminate
cross-reactions with others species and with other immunoglobulin
classes for double labelling.

Both dyes can be used with LiCor Odyssey imaging systems and all the
high-sensitivity techniques listed above. We recommend that the
antibodies be diluted at least 1:50,000 to 1:200,000 due to the high
sensitivity of the conjugates.

Stratech Scientific is
a distributor of high quality, competitively priced, reliable products
for research laboratories throughout the UK and Europe. Please contact
us to find out which ranges we can supply in your country.