Phospho-screen plus

Overview

This service enables a global view of protein and site specific phosphorylation events using a workflow optimized for a 24hr (8 x 3hr) LC-MS/MS analysis. This workflow can be used to study phosphorylated species from complex samples such as cell lysates, tissues and biofluids. Samples are processed by trypsin digestion, peptides are fractionated using a basic reverse phase strategy. Phosphorylated peptides are enriched using titanium dioxide microparticles. This service uses 24hours of instrument time versus the 2hrs used for Phospho-screen. The increased instrument time is reflected in the number of phospho protein/peptide identifications.