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Notes: A reporter construct was assembled in the pGL3 Control Vector downstream of the Xba I consisting of the 3'UTR of the COX-2 gene. The construct was designed to see if the UTR had an affect on mRNA stability in the presence of IL-1beta. The construct as well as the pSV-Beta Galactosidase Control Vector were transfected into A549 cells using the Tfx™-50 Reagent. A lot of detail is provided for the transfection. The reporter activities were monitored with the Luciferase Assay System and the Beta-Galactosidase Enzyme Assay System. (0606)

Notes: Luciferase (prepared in pGL2-Basic Vector) and β-galactosidase (pSV-β-Galactosidase Control Vector) reporters were studied in SL2 Drosophila cells, HeLa cells and primary rat cortical neurons. Transfections into the SL2 and HeLa cells were accomplished with standard calcium phosphate methods. Transfection of primary rat cortical neurons was accomplished with the Tfx™-50 Reagent as described in Boeckman, F.A. et al. (1996) Neural Notes II(1), 13-15. Reporter assays were performed with the Luciferase Assay System and the β-Galactosidase Assay System. (0890)

Notes: DNA-liposomes were injected intraveneously with a RSV-luciferase expression vector. Forty-eight hours post injection, the animal was sacrificed and the heart, lung and ~300mg of liver was homogenized in Promega's Luciferase Cell Culture Lysis Reagent with the aid of zirconium beads. The debris was centrifuged away and the supernatant analyzed for luciferase activity with the Luciferase Assay System. (0199)

Notes: The 293 cell line was cotransfected with a luciferase reporter containing the IL-2 promoter, an expression vector for either EGR-1, NFATc or both and finally the pRL-TK Vector. Reporter to control ratio was 4:1 and the luciferase activity was determined with the Dual-Luciferase® Reporter Assay System. (1265)

Notes: The RNasin Ribonuclease Inhibitor was used to protect RNA transcripts during primer extension analysis and in vitro transcription of RNA probes. The resultant RNA probes were used for RNase protection assays. (1639)

Notes: The Serine/Threonine Phosphatase Assay System was used to assess protein phosphatase 2A, 2B and 2C activity in nuclear extracts of Human umbilical vein cells (HUVEC) with or without lysophosphatidylcholine treatment. Gel shifts were also performed with nuclear extracts prepared from the HUVEC cells using the SP1 Consensus Oligonucleotide. Luciferase reporter studies were also performed in HUVEC cells using pGL3 Basic-derived vectors and the Luciferase Assay System. (1305)

Notes: A NF-kappaB luciferase reporter vector was constructed with the TATA box and transcription start site of the rabbit beta-globin promoter and three tandem repeats of the NF-kappaB consensus site in the pGL3 Basic Vector. Another vector was constructed with mutated NF-kappaB sites. The reporter vector was transfected into primary human umbilical vein endothelial cells with the Tfx™-50 Reagent. No details of the transfection conditions were provided. Luciferase activities were monitored with the Luciferase Assay System. (0418)

EMBO J.16, 659-671.
A small heat shock protein stably binds heat-denatured model substrates and can maintain a substrate in a folding-competent state.1997

Lee, G. J. , Roseman, A. M. , Saibil, H. R. , Vierling, E.

Notes: The Quantilum™ Recombinant Luciferase was heat denatured in the presence of heat shock protein hsp 18.1 and the renaturation of the protein was assayed in with the Luciferase Assay System. The denaturation was performed in either Rabbit Reticulocyte Lysate or Wheat Germ Extract in the presence or absence of added ATP. (0809)

J. Biol. Chem.272, 21045-21051.
A type I interferon signaling factor, ISF21, encoded on chromosome 21 is distinct from receptor components and their down-regulation and is necessary for transcriptional activation of interferon-regulated genes.1997

Notes: Luciferase and CAT assays were conducted in CHO-K1 cells using constructs prepared in the pGL3-Basic and pCAT®-Basic Vectors, and the activity was normalized to β-galactosidase activity supplied by the pSV-β-Galactosidase Control Vector. The pCAT® Vectors have been replaced by the next-generation pCAT®3 Vectors. (1055)

Notes: Luciferase reporter constructs containing either the luc or luc+ gene (from pSP-luc+NF Fusion Vector) were used in in vitro transcription to produce capped mRNA. Luciferase was produced by in vitro translation using either neurospora extract or Promega's Rabbit Reticulocyte Lysate, Nuclease Treated and detected using the Luciferase Assay Reagent. (0208)

J. Biol. Chem.272, 12692-12698..
Cloning and characterization of the promoter region of a gene encoding a 67-kDa glycoprotein.1997

Chatterjee, N. , Zou, C. , Osterman, J. C. , Gupta, N. K.

Notes: Luciferase studies were performed in KRC-7 rat hepatoma cells using constructs prepared in the pGL3 Basic Vector. The luciferase constructs were cotransfected with the pSV-beta-galactosidase Vector. Luciferase activities determined with the Luciferase Assay System were normalized to readings from the Beta-Galactosidase Enzyme Assay System. (1364)

Notes: Reporter studies were performed in human embryonic kidney 293 cells using constructs prepared in the pGL2-Basic Vector. The Erase-A-Base® System was used to create unidirectional deletion mutants of promoter elements. (0236)

Notes: Reporter studies were performed in HepG2 cells. The experimental constructs were made using the the pGL3 Basic Vector and luciferase activities were monitored with the Luciferase Assay System. (0669)

Notes: Luciferase studies were performed in mouse embryo fibroblasts. Experimental constructs were prepared in the pGL2-Basic Vector and luciferase activities determined with the Luciferase Assay System. (0861)

Notes: Total RNA was isolated from adrenomedullary tissue dissected from areas near to or far from the adrenal cortex. The isolated RNA was used for RT-PCR. Reporter studies were performed in Neuro2a murine neuroblastomas, SH-SY5Y human neuroblastomas and bovine chromaffin cells. All luciferase activities were normalized to control beta-Galactosidase activity. The consensus oligonucleotides were used in gel shift assays of bovine chromaffin cell nuclear extracts. The oligonucleotides were used to show specificity for Egr-1. (1614)

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