As Nick Fury and Tony Stark analyze Mysterio's equipment, Fury notes that he is allowing this Peter so much leeway as a way to make up for his failure to protect the Peter he knew. Peter attempts to explain himself to Gwen and May, but it takes Miles arriving to back up his story and convincing them that he is not an impostor or out to play a sick joke on them. Talking with Gwen and Miles in his attic, Peter confirms that Gwen exists in his world without mentioning that her counterpart is dead and also mentions his relationship with Mary Jane, while he in turn is shocked to hear that his counterpart dated Kitty Pryde, although Miles is left curious whether he has a counterpart in Peter's world. As Fury arrives to pick Peter up, Peter sees Mary Jane watching him in the street, but she runs away before he can talk to her. Returning to the Helicarrier, Peter learns that Stark is having trouble determining how he can identify Peter's world of origin amid the multitude of alternate universes in existence. With Miles' help, the Ultimates determine the location of Mysterio's headquarters based on the energy generated by his equipment, prompting the heroes led by Peter to suit up and head for the base.[6]

Peter is shown to be learning the 'Way of the Spider', from Shang-Chi, and also seen neutralizing a couple of robberies. Jackal is revealed to be working on a scheme, recruiting several spider-powered criminals for some kind of project. Later, Peter and Carlie Cooper are talking when Carlie reveals she has spider-powers. When news of several hundreds of New Yorkers having manifested spider-powers reaches them, Carlie web swings away to help. Peter then pretends to be another random spider-powered New Yorker and defends New York against the spider-powered villains, along with the Avengers. Anti-Venom is seen curing people of their spider-powers, as he showed the ability to do so in New Ways to Die. Jackal is seen working on the Spider-King, filled with tiny embryos. Horizon Labs and Mr Fantastic work on finding a cure while the Avengers keep Manhattan quarantined to stop the virus from spreading. While at an lab, Peter and Carlie are attacked by Chance, Scorcher, and White Rabbit, who also have spider-powers. Peter manages to defeat them using his training from Shang-Chi. Jackal has the battle taped and gives it to Tarantula to study, as he will soon be 'tested'. Reed Richards finally develops a vaccine that stops people from gaining spider-powers, but cannot cure those already infected. J. Jonah Jameson discovers that he, too, has gained spider powers. Anti-Venom continues curing people, however Madame Web faints from the massive excess of spiders accessing the 'web of life'. Carlie meets up with Spider-Man, when they attempt to take down Shocker, who has grown 6 extra arms. After taking off his mask, they find that his face is slowly mutating into a spider's face, and his body is doing the same. Carlie starts to mutate as well.The Spider-Queen tells Jackal that the people of New York will soon become spiders, and she will own New York.

The practice may have originated in a Celtic festival, held on 31 October–1 November, to mark the beginning of winter. It was called Samhain in Ireland, Scotland and the Isle of Man, and Calan Gaeaf in Wales, Cornwall and Brittany. The festival is believed to have pre-Christian roots. After the Christianization of Ireland in the 5th century, some of these customs may have been retained in the Christian observance of All Hallows' Eve in that region—which continued to be called Samhain/Calan Gaeaf—blending the traditions of their ancestors with Christian ones.[2][3] It was seen as a liminal time, when the spirits or fairies (the Aos Sí), and the souls of the dead, could more easily come into our world.[4] It was believed that the Aos Sí needed to be propitiated to ensure that the people and their livestock survived the winter.