Eukitt mounting medium

From:

Cathy Mayton

I would like to give my 2 cents about Eukitt mounting medium.

I have used Eukitt mounting medium exclusively over the past 4.5 years in
my private contract lab and even longer when I was employed in the Bone
& Joint research lab in Salt Lake City Utah. Over the past 4.5
years I have mounted sections embedded in paraffin as well as glycol
methacrylate (GMA) with no problems. I have not received any calls
from my clients complaining that they could no longer read the slides.

Sections mounted with Eukitt must be placed in xylene prior to
mounting and Eukitt must be diluted with xylene as well. You
did not state if a xylene substitute was being used. It is
difficult to trouble-shoot where the problem is because the staining
protocol or how the sections were handled prior to mounting with Eukitt
was not mentioned.

I do not have the luxury of going back to old slides because they are
routinely sent to the client once the study is completed. However,
this weekend I was able to pull some slides that were archived for one of
my clients. The slides were paraffin embedded sections stained with
H&E and mounted with Eukitt that were completed in January of this
year. The slides are still just as crisp as the day they were
stained and mounted.

I purchase my Eukitt direct from Calibrated Instruments. I think it
is important that if we are having a problem with a product than we
should call the manufacturer of the product. This gives them a
chance to address a problem that may be a manufacturing problem or they
can help trouble-shoot a problem associated with the labs
protocol.

I am confident that if you called Mr. Bruce Shroyer at Calibrated
Instruments he would be more than happy to discuss the problem associated
with your slides. Mr. Shroyer can be reached at 914-741-5700 and is
located in Hawthorne, New York.

Last year and into the early part of this year, I started having a
problem with a product. The first thing I did was trouble-shoot my
protocols to determine if there was something I was doing to cause this
problem. After thoroughly trouble-shooting every angle of the
protocol I then called the manufacturer of the product. After
awhile it was determined that yes, it was a manufacturing problem.
The manufacturer took care of me by replacing the product and I was a
happy camper.

Many vendors monitor Histonet and go out of their way to help us in our
day to day processing problems. So the next time a problem
arises, please be considerate to our vendors and give them a chance to
address any problems you may have that could be associated with their
products.