Background and aim: The purpose of this study is to compare the effect of Ledermix® paste and green tea extract on the concentration of inflammatory mediators.

Materials and methods: In this in-vitro experimental study, first, the noncytotoxic concentrations of Ledermix® medicament and green tea extract were determined by MTT assay. Afterwards, the concentrations of IL-1β, IL-6, and TNF-α inflammatory mediators were assessed by ELISA test in the four experimental groups and also in the control group at different time intervals of 2, 24, and 48 hours and after 7 days. One-way analysis of variance (ANOVA) was used for statistical analysis of the obtained raw data followed by Tukey's post-hoc test for pair comparisons.

Results: the noncytotoxic concentration of green tea extract and Ledermix® paste equaled 12 µg/ml and 0.25 µg/ml, respectively. Time interval had no significant effect on the concentration of the mediators. Generally, both substances significantly decreased the concentration of IL-1β and IL-6 inflammatory mediators in comparison with the control group.

Conclusions: Green tea extract is less cytotoxic and more biocompatible than Ledermix® paste. Both Ledermix® paste and green tea extract expressed anti-inflammatory effects.