The authors describe how qPCR reactions to examine a variety of housekeeping genes from cDNAs derived from different human tissues were set up on the Bravo Automated Liquid Handling Platform and by hand.

Data is included demonstrating how dilutions performed by each method showed similar slopes and good linearity, and targets amplified by either method also gave similar Ct values.

When examining cDNAs from fetal and adult lung, the authors observed differential expression in the B2M, whereas GUSB and TBP remained similar.

The application data demonstrates that the Bravo Automated Liquid Handling Platform should be useful to scientists interested in speeding up setup while maintaining reliability when performing qPCR studies.