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12.04.10

Coagulase-negative staphylococci isolated from bovine extramammary sites and intramammary infections in a single dairy herd

Coagulase negative staphylococci (CNS) isolates were obtained from extramammary swab samples on 66 dairy cows (perineum, udder skin, teat apices and streak canal) and compared with bovine mastitis isolates. Species identification was performed by phenotyping (API) and ribotyping. Staph succinus and Staph xylosus were the most common species isolated from extramammary sites, while Staph chromogenes and Staph simulans were the most common species obtained from cases of bovine mastitis. The predominant CNS species from extramammary sites were seldom isolated from mastitis samples while S. simulans was shown to be a specific udder pathogen, in contrast to other CNS species.