[Objectives] In order to isolate lignocellulosic bacteria and find important enzyme and pathway of lignocellulose degradation from its genome,the present study was designed to isolate high-performance lignocellulosic bacteria from the soil.[Methods] Cellulose was used as the sole carbon source to isolate strains with high cellulose enzyme activities from rotten leaves. The cellulose-degrading activities were measured,and the complete genome were sequenced on the MiSeq platform. Taxonomy of strain J1 was investigated through the comparisons of its genomic sequencing with five available genomes from other bacteria. Whole genome of strain J1 was annotated by blasting on COG databases,CAZy databases and so on.[Results] Over 27 cellulose-degrading bacteria were isolated and strain J1 was finally selected. The peak value of endocellullase,filter paper enzyme activity and β-glucosidase were 0.39,0.18 and 0.11 IU·mL-1,respectively. Strain J1 was primarily identified as Pseudoxanthomonas sp. The predicted lignocellulose-degrading genes on the genome of strain J1 include β-glucosidase gene and endo-β-1,4-glucanase gene and so on. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed strain J1 could convert cellulose to ethanol.[Conclusions] Strain J1 has high lignocellulose-degrading activity and may utilize cellulose to produce ethanol. Strain J1 is a candidate for ethanol fermentative production and its genomic information provided insights into lignocellulose-degrading pathways in the environment.