Test organisms

Study design

Test conditions

Test temperature:

20 ± 2°C

pH (if soil or dung study):

Soil pH ranged from 6.19 to 7.64 for the beginning of all tests and from 3.92 to 7.40 for the end of all tests.

Humidity:

Soil moisture content of all treatments in all tests was independent of DEG concentrations and ranged from 59 to116% of the soil water holding capacity (WHC) for the beginning of all tests and from 44 to 91% of the soil WHC for the end of the invertebrate tests.

0, 200, 400, 600, 800, 1600, 3200, 6400, 12800, 25600, 51200 mg/kgThe recovery of DEG from the beginning of the range-finding tests was good and ranged from 75 to 90%. DEG recovery on Day 0 of the northernwheatgrass test was similar among all but the lowest (200 mg/kg) treatments and ranged from 85 to 102%. The recovery in the lowest treatment was below the detection limit. There was evidence of loss of DEG from soil over time; measurable concentrations of DEG were only detected in the highest concentration (25600 mg/kg) at Days 7 and 21 of the test. In addition, the % DEG recovery in these concentrations also decreased over time with 102, 74 and 63% recovery at Days 0, 7 and 21, respectively.

Adult Folsomia candida survival was affected adversely following exposure to DEG in AS with a chronic LC50 of 15,689 mg/kg. Chronic reproduction appeared to follow a concentrationdependent pattern and the IC50 for number of progeny produced was 7508 mg/kg.Adult survival, no. progeny produced

Applicant's summary and conclusion

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