Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications

The antibody reacts with the Fab portion of human IgM.

Derivation

Animals were immunized with purified human IgM.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against human IgM

Cellular Products

immunoglobulin; monoclonal antibody; against human IgM

Comments

The antibody reacts with the Fab portion of human IgM.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.