The research described in this thesis aims to develop new strategies for the synthesis of fine chemicals by replacing conventional
methods by nature-inspired technologies. The integration of multiple enzymatic steps into one-pot cascade processes is the
ultimate goal to convert cheap renewable sources into valuable compounds and limit the production of waste.Hereby multi-enzymatic
cascade reactions are developed for the synthesis of natural and unnatural carbohydrates. The highly valuable dihydroxyacetone
phosphate is the key intermediate in these cascades. The thermodynamic control is based on the formation and hydrolysis of
phosphorylated intermediates by acid phosphatase and the synthetic key step is the C-C bond formation by DHAP-dependent aldolases.
Possibilities of and limitations on the use of alkaline phosphatase and transketolase are also investigated.Another important
part of this research is the implementation of such already established cascades with a more efficient process. This is based
on the immobilization of the enzymes and their use in different types of packed-bed reactors. This allows to switch from one-pot
batch methods to the labour-free technology of continuous flow systems for the synthesis of highly valuable compounds.

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