Lab prep, media, and supplies

Introduction

This information is primarily for the course TAs and media
prep person, but might be of some interest to the students. All
amounts listed below are based on a class of 40 students in 2 lab sections.

Media Schedule

TENTATIVE - Check weekly against supplies.

Deadline

Media

Type

Number

Make

Jan 18

PYD

plates

50

1.5 liters

BIM

tubes

50

1.5 liters

E

tubes

50

1 liter

YPD-Amp

tubes

50

0.5 liter

NA

plates

100

3 liters

NAM

tubes

50

0.5 liter

Jan 25

PYD

plates

50

1.5 liters

BIM

tubes

50

1.5 liters

YPD-Amp

tubes

50

0.5 liter

NA

plates

100

3 liters

NAM

tubes

50

0.5 liter

Feb 01

PYD

plates

50

1.5 liters

BIM

tubes

50

1.5 liters

BIM

plates

50

1.5 liters

E

tubes

50

0.5 liters

YPD-Amp

plates

50

1.5 liters

YPD-Amp

tubes

50

0.5 liter

NA

plates

100

3 liters

NAM

plates

50

1.5 liters

NAM

tubes

50

0.5 liter

Feb 08

PYD

plates

100

3 liters

BIM

plates

50

1.5 liters

E

tubes

50

0.5 liter

YPD-Amp

plates

50

1.5 liters

NA

plates

100

3 liters

NAM

plates

50

1.5 liter

Feb 15

BIM

plates

50

1.5 liters

E-agar

plates

50

1.5 liters

PYD

plates

50

1.5 liter

YPD-Amp

plates

50

1.5 liters

NAM

plates

50

1.5 liters

Feb 20

BIM

plates

50

1.5 liters

YPD-Amp

plates

50

1.5 liters

E-agar

plates

50

1.5 liters

BIM

plates

50

1.5 liters

NAM

plates

50

1.5 liter

Mar 01

BIM

plates

50

1.5 liters

E-agar

plates

50

1.5 liters

Mar 08

NA

plates

150

4 liters

Media preparation

PYD plates

Add to 1 liter of distilled water:

2g peptone

2g yeast extract

5g dextrose

15g agar

Mix & autoclave. Cool to about 50C, mix, & pour into plates

YPD-Amp

Add to 1 liter of distilled water:

20 grams dextrose

10 grams peptone

5 grams yeast extract

Adjust to pH 5.0 using dilute HCl

For tubes dispense 10ml into each of about 100 16x150mm tubes, cap (USE YELLOW CAPS) & autoclave. Allow to cool, and add 40ul of 25mg/ml ampicillin to each tube. This ampicillin stock can be made by mixing 0.25g ampicillin-HCl to 10ml distilled H2O, dissolve, and filter sterilize (store frozen).

Note that PYD (above), TSA (trypticase soy agar), YT, LB, or any other basic rich agar can be substituted for NA for the purposes of this class.

NAM media

Nutrient agar for preparing this media (Nutrient Agar/mineral water, or NAM) is supplied in pre-mixed form - prepare as directed. The only difference is that instead of distilled water, use Pelligrino mineral water.

Note that PYD, TSA (trypticase soy agar), YT, LB, or any other basic rich agar can be substituted for NA for the purposes of this class. Likewise, and high mineral content mineral water can also be substituted.