As a part of investigation to use the Anatrctic krill, Euphausia superba, more effectively as a food source, processing conditions, utilizations and storage stability of krill paste (intermediate product of krill) were examined and also chemical compositions of krill paste were analyzed. Frozen raw krill was chopped, agitated with of water to the minced krill and then centrifuged to separate the liquid fraction from the residue. This liquid fraction was heated at for 20 min. to coagulate the proteins of krill, and it was filtered to separate the protein fraction. Krill paste was prepared with grinding the protein fraction, adding of polyphosphate and of sodium erythorbate to the krill paste for enhancing of functional properties and quality stability. The krill paste was packed in a carton box, and then stored at . Chemical compositions of krill paste were as follows : moisture , crude protein , crude lipid , and the contents of hazardous elements of krill paste as Hg 0.001 ppm, Cd 1.15 ppm, Zn 9.1 ppm, Pb 0.63 ppm and Cu 11.38ppm were safe for food. The amino acid compositions of krill paste showed relatively high amount of taurine, glutamic acid, aspartic acid, leucine, lysine and arginine, which occupied of total amino acid and also taurine, lysine, glycine, arginine and proline were occupied of total free amino acid. Fatty acid compositions of krill paste consist of of saturated fatty acid, of monoenoic acid and of polyenoic acid, and major fatty acids of product were eicosapentaenoic acid (), oleic acid (), palmitic acid (), myristic acid () and docosahexaenoic acid (). In case of procssing of fish sausage as one of experiment for krill paste use, Alaska pollack fish meat paste could be substituted with the krill paste up to without any significant defect in taste and texture of fish sausage, and the color of fish sausage could be maintained by the color of krill paste. Judging from the results of chemical and microbial experiments during frozen storage, the quality of krill paste could be preserved in good condition for 100 days at .

Since a long time ago, more than thirty kinds of fermented fish product have traditionally been favored and consumed in Korea. In general, they fermented with of sodium chloride. However, it has been currently known that sodium chloride is one of causative ingredient for adult diseases. For that reason, reduced sodium salt diet is recently recommended in developed countries. This study was attempted to process low sodium salt fermented fish using anchovy, Engraulis japonica, and yellow corvenia, Psedosciaena manchurica, as raw materials with partially replacing the sodium salt with potassium chloride. The most favorable taste for fermented anchovy and yellow corvenia were revealed at 60 and 90 days fermentation, respectively. Judging from sensory evaluation with variance of analysis and orthogonal contrast method, little difference of taste were found when sodium salt was replaced with KCl even by as compared with conventional fermented fish. Taste for low salt fermented anchovy and yellow corvenia were the most favorable when they were prepared with salt, KCl, sorbitol, lactic acid and alcohol extract of red pepper as preservatives and flavor enhancers.

A survey was made to determine seasonal variation of histamine, total volatile base and trimethylamine levels in a number of fresh fish and canned fish products as available through present distribution systems in Seoul area in order to assess potential danger of histamine poisoning related problems. Data obtained indicated that those products as available in present distribution systems should present no potential danger of histamine poisoning related problems.

To investigate potential application of radurization technique as an effective tool for controlling histamine accumulation, histamine contents were determined in fresh Korean mackerel meat samples irradiated at six dose levels of gamma radiation up to 1 Mrad during 35 day-storage at . Total bacterial counts, total volatile bases and trimethylamine contents were also estimated. The minimum and maximum doses for Korean mackerel were determined to be 0.2 and 0.5 Mrad, respectively. Irradiation induced redening of fish muscle tissue and redening appeared to be dose dependent. At low doses, this redening contributed positively to the overall sensory qualities. At 0.5 and 1.0 Mrad, on the other hand, meat samples were excessively redened to the extent that red and white muscle layers were not readily distinguishable. No appreciable histamine accumulation occured during entire 35 day-storage in fresh mackerel meats, whether irradiated or not, when they were kept at ice temperature. At , histamine started to accumulate slightly during late storage in the samples irradiated at low doses. Even in the unirradiated samples, the histamine accumulation did not exceed the level of 10.0 mg per 100g meat until the 28th day at . At , however, histamine accumulation in the unirradiated was phenomenal from very early storage and the values reached 827.5 mg per 100g meat by the 35th day, while histamine accumulation in the irradiated was severely suppressed. Irradiation invariably brought about a significant reduction of initial microflora, disrupting normal spoilage pattern. This was reflected in the suppression of not only histamine but also total volatile bases and trimethylamine accumulation during post-irradiation storage at refrigerated temperatures.

The present study was carried out for making clear the organic acids composition of some shellfishes and their boiled-dried products. Three species of shellfish such as top shell, hard clam and abalone were chosen as the experimental samples and analysed for their nonvolatile organic acids composition by gas liquid chromatography (GLC). Eight kinds of nonvolatile organic acid were identified in raw and boiled-dried top shell, major ones were succinic, malic and pyroglutamic acid. Out of nine kinds of nonvolatile acid was determined in raw and boiled-dried of hard clam, abundant ones were succinic, lactic and malic acid, And seven kinds of nonvolatile organic acid were detected in boiled-dried abalone while eight kinds in raw sample, abundant ones were succinic, lactic and pyroglutamic acid, respectively. In all samples, the total amount of nonvolatile organic acid was the most abundant in hard clam while the least in abalone, and the content of succinic acid was appeared as the most abundant one. In dried samples, the total amount of the nonvolatile organic acid was determined to be about half-decrease after the boiled-dried treatment of the raw samples, of which abalone showed the highest decreasing을 rate whereas hard clam was the lowest. In general, the decreasing rate of each acid was the higher in fumaric, malic and citric acid than in maleic, oxalic and succinic acid.

The mechanics of the walking shrimps is useful to clarify the fishing mechanisms in relation to the fishing gears. The gaiting behaviour concerning step positions and step timings on the flat board and the nettings, 16, 23 and 37mm in mesh size were experimented in the aquarium using video camera from June to October, 1984. It was found that the irregular movements of walking legs in step positions and step patterns were appeared on the nettings more than the flat surface due to the absence of mechanical contact with the substrates. The mean stride length and coefficient of variation of the periods in the walking shrimps on the flat board were significantly different from those values on the nettings, However, the velocity, the period and the ratio of forestroke to backstroke were unsteadily changed with the carapace length, and showed little difference under the four conditions. The mean phase difference on the flat board was greater than those values on the nettings which were decreased, while standard deviation on the flat board was smaller than those values on the nettings which were increased with increasing in mesh size.

The mechanism of reproductive cycle of longchin goby Chasmichthys dolichognathus was investigated for the annual variations of gonadosomatic index(GSI), histological changes of gonad, reproductive cycle, and initiating and terminating factors of the breeding season. The experimental fish were captured in the tide pool of Tongbaeksom, Pusan, Korea from February 1983 to September 1984. Experimental fish for the study of initiating and terminating factors of the breeding season were maintained in filtered recirculating aquariums and exposed to various temperature and photoperiod regimes. The ovary is a pair of sac-shaped organ, and the testicular structure is tubular and it is connected to the seminal vesicle which is located at the posterior end of the testis. GSI began to increase from February when the water temperature began to increase and reached peak in April. It began to decrease from August, the top water temperature season, and maintained relatively low values until January. The annual reproductive cycle includes successive six developmental stages : early growing from December to February, late growing from January to March, early mature from February to May, late mature and ripe from March to August, spawning from April to July, degeneration and resting stage from May to December. An experimental study based on the reproductive cycle of this species indicated that rising temperature under long photoperiod during spring stimulated gonadal maturation, whereas terminating factor of the breeding season with recrudescence of the gonads was demonstrated as high water temperature during summer regardless of photoperiod.

The reproduction of marbled sole Limanda yokohamae, caught near around the southeastern coast of Korea, from December 1983 to November 1984, was investigated based on such annual variations as gonadosomatic index(GSL), gametogenesis, reproductive cycle, spawning number, hepatosomatic index (HSI), and fatness. GSI began to increase in the autumn season with the onset of shorter day length and colder water temperature, and reached the maximum value in December with the shortest day length and the lowest temperature over the year. The gonad activated the proliferation of oogonia and spermatogonia in June, reached the mature stage in October, ripe in December, and spawning from the end of December to January. After spawning, it showed the resting stage which gonad remained regressive and suppressive from February to May. In addition, the adult individuals observed discharged eggs only once during their spawning period. At yolk globular stage, the substance of vitellogenin synthesized from the liver was considered to participate in the active yolk accumulation of oocytes. Marbled sole was concluded to be a typical winter spawning species in that such environmental factors as short day length and low water temperature were closely related with the gametogenesis, the stimulation of oocyte maturation, and were also affecting the ovulation.

Limanda yokohamae was studied on its growth, age at maturity, fecundity and survivorship, based on the specimens off the southeastern coast of Korea from December 1983 to November 1984. The total lengths at the formation of annulus on the otolith were back-calculated. The age at the first reproduction is 2yrs in both sexes. Fecundity was estimated to be . Annual survival rate is 0.435 in female and 0.335 in male.

In order to obtain the fishing possibility by acoustic, the two fishes, Lateolabrax japonicus, Mugil cephalus, were bred in a water tank. The feeding sounds from the fishes and the artificial sounds were recorded by a hydrophone and then the frequency and the sound pressure level of the sounds recorded were analyzed by the digital frequency analyzer. These sounds were edited in two manners of which one is emitted for 10 seconds and paused for 10 seconds continuously and the other is emitted for 20 seconds and paused for 20 seconds also. These edited sounds were emitted again into the tank and the respose of fisher were observed. Lateolebrax japonicus showed a positive response and Mugil cephalus responsed a little positively to the emitted feeding sound, The fishes seemed to show a positive response only in emitting a moderate pressure level of feeding sound. Lateolabrax japonicus and Mugil cephalus showed negative response to the emitted artificial sound. It was most effective to increase the sound pressure level that the fishes went away from the sound source to the emitted artificial sound.

As the fundamental studies for the population of Myagropsis myagroides, the effect of plant hormones on the regeneration for the primary, secondary lateral and receptacle segments were investigated. At high concentration() of plant hormones inhibits from regenerating and lower concentrations advance the regeneration. The most effective concentrations of hormones on the primary lateral segments are of kinetine, IAA, NAA and of 2, 4-D in order, meanwhile in the secondary lateral segment of NAA, of kinetine, of IAA, and of 2, 4-D in order. The net regeneration rates of primary and secondary lateral segments which are measures by the wet weight are , respectively. The influence of apical dominanace on the primary lateral segments decreases, on the other hand, increases in the secondary lateral segments because of the distance from the apex, Especially, the secondary lateral segments treated with below plant hormone are matures with receptacle of small branching by the regenerated branching.