In the presented study, low temperature combustion was established with a direct injection of diesel fuel being a representative of high reactivity fuels and tire pyrolysis oil being a representative of low reactivity fuels. Tire pyrolysis oil was tested as a potential waste derived fuel for low temperature combustion, as it features diesel-like physical properties and lower cetane number compared to diesel fuel. The goal of this study was determination of suitable injection strategies and exhaust gas re-circulation rates to explore potentials of both fuels in reducing emissions in low temperature combustion modes. It was demonstrated that relatively small changes in the engine control strategy possess the potential to significantly improve NOx/particulate matter trade-off with minor effect on engine efficiency. In addition, low temperature combustion was for the first time successfully demonstrated with tire pyrolysis oil fuel, however, it was shown that lower re-activity of the fuel is by itself not sufficient to improve NOx /soot trade-off compared to the diesel fuel as entire spectra of fuel properties play an important role in improving NOx /soot trade-off. This study thus establishes relations between different engine control strategies, intake manifold pressure and exhaust gas recirculation rate on engine thermodynamic parameters and engine-out emissions while utilizing innovative waste derived fuel that have not yet been analysed in similar combustion concepts.