To develop a new utilization for Japanese cedar (Sugi : Cryptomeria japonica D.Don), we inverstigated the bioactivities of sugi extractives, superoxide dismutase (SOD) mimic and angiotensin I-converting enzyme (ACE) inhibitory activities. The ethanol extract from heartwood and outer bark showed the highest SOD mimic sctivity and ACE inhibitory activity, respectively, among 24 extracts prepared from roots, leaves, heartwood, sapwood, inner bark, and outer bark by successive extraction with n-hexane, chloroform, ethanol, and hot water. Isolation of active compounds from each extract was attempted by HPLC equipped with a reversed phase column, and identification of the bioactive compound was achieved by GC-MS and ^1H-NMR.As the results, we isolated and identified sequrin-C from the heartwood as a SOD mimic active compound, and catechin and procyanidin B3 as ACE inhibitors from the outer bark.本学上阿多古演習林に生育するスギ(樹齢15年、樹高13m)を外皮、内皮、心材、辺材、葉、根に分別し、それぞれをヘキサン、クロロホルム、エタノール、熱水で逐次抽出した24画分について各種生理活性を検索した結果、SOD様活性については心材エタノール抽出画分が、ACE阻害活性については外皮エタノール抽出画分が最も高い活性を示した。そこで、それぞれの画分に含有される活性物質を逆相HPLCを用いて単離・精製し、^1H-NMR及びGC-MS分析に供して活性物質の同定を行った。その結果、スギ抽出物のSOD様活性物質はセキリン-Cであり、ACE阻害活性物質はカテキンとプロシンアニジンB3であることを明らかにした。