[Histonet] Eosin in processor,GMS m-wave, onion

From:

JColCLEFA@aol.com

We've used eosin in the processor, but I don't know if that will affect Q FE
or not
When I do Microwave GMS I nuke the slides in the silver solution to give the
tissue the benefit of the M-wave treatment, otherwise you're just heating
the solutions. I don't nuke the slides in the Chromic though as this seems to
blast the tissue off the slides. Also I nuke for 15 seconds, remove the
continer,- agitate the solution gently with a plastic pipette, and nuke again for 10
sec. and agitate again. this assures the solution is a constant temp throughout
and not hot on the top/cool on the bottom. Also are your solutions
consistently at the same temp each time you begin the stain? I have different times
listed depending on whether the chems are cold from refrigeration or have been
standing @ RT.
in addition to the root tips, the onion has a great tough membrane covering
the flat parts inside (the leaves of the bulb?) that under the scope shows the
cells mighty nicely.
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