The effect of platelet-rich plasma formulations and blood products on human synoviocytes: implications for intra-articular injury and therapy

Hillary J Braun, Hyeon Joo Kim, Constance R Chu, Jason L Dragoo

American Journal of Sports Medicine 2014, 42 (5): 1204-10

24634448

BACKGROUND: The effect of platelet-rich plasma (PRP) on chondrocytes has been studied in cell and tissue culture, but considerably less attention has been given to the effect of PRP on synoviocytes. Fibroblast-like synoviocytes (FLS) compose 80% of the normal human synovium and produce cytokines and matrix metalloproteinases that can mediate cartilage catabolism.

METHODS: Peripheral blood was obtained from 4 donors and processed to create LR-PRP, LP-PRP, RBCs, and PPP. Human synoviocytes were cultured for 96 hours with the respective experimental conditions using standard laboratory conditions. Cell viability and inflammatory mediator production were then evaluated.