Sample_hyb_protocol: Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip YeastS98 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.

Sample_scan_protocol: GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.

Sample_description: We have examined the transcriptional response of S. cerevisiae to exposure to pyocyanin at 25 and 250 ?g/ml concentrations. Our analysis indicates that the changes are consistent with an oxidative stress response, as observed in other studies of the mode of action of pyocyanin toxicity.

Sample_data_processing: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.