25-OH Vitamin D3/D2 by HPLC (Reversed Phase)

The Bio-Rad 25-OH Vitamin D3/D2 by HPLC assay provides precise results for circulating 25-OH Vitamin D3 and 25-OH Vitamin D2 levels. This improved application can be used for simultaneous measurement of the discrete values of 25-OH Vitamin D3 and 25-OH Vitamin D2. The HPLC method allows for precise monitoring of response, useful for daily routine analysis to therapy in patients.

Bio-Rad provides customers with a human serum based calibrator, containing defined Vitamin D3 and D2 values traceable to the Vitamin D Standard Reference Material® 972 from NIST (The National Institute of Standards and Technologies, an agency of the U.S. Department of Commerce).

Vitamin D is an essential bio-regulator of calcium metabolism and thus significant for bone mineralization. Vitamin D deficiency is a recognized cause of secondary hyperparathyroidism, increased bone turnover and bone loss (ostepenia) in eldery people. Therefore, Vitamin D testing is essential in the management of patients with various disorders of calcium metabolism.

Recent studies have suggested that Vitamin D may play a role in protecting against cancer and in regulating normal immune function. Deficiency has been linked with autoimmune conditions such as type 1 diabetes, multiple sclerosis and rheumatoid arthritis. Vitamin D supplementation to prevent and treat deficiency can be provided in the form of 25-OH Vitamin D3 or 25-OH Vitamin D2. Serum concentration of 25-OH Vitamin D has been accepted as the clinical indicator of a patient's Vitamin D status. In order to identify and correct the deficiency of these 25-OH metabolites, an accurate measurement is essential.

Test characteristics:

Principle: Isocratic HPLC method (Reversed Phase)

Flow rate: 1.1 mL/min

Duration of chromatography: 12 minutes

UV Detection: 265 nm

Sample volume: 500 µL serum or plasma

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195-6527
Serum calibrator set containing 25-OH vitamins D3 and D2, plus vitamin A, for HPLC assay (3 x 3 mL); traceable to the Vitamin D Standard Reference Material® 972 from NIST (The National Institute of Standards and Technologies, an agency of the U.S. Department of Commerce).