Wheater’s Functional Histology 5th

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4.4 Scanning electron microscopy Flat-mounted retinas, retinal cross-sections preparations, and isolated retinal cells were used for scanning electron microscopy (SEM). In the flat retina, the inner limiting membrane (ILM), constituted by the feet of the Müller cells [11] need to be picked off in order to clearly observe the RGCs and their membrane surface. To remove the ILM, we used a technique described in detail previously [12]. Briefly, a 2.5% poly-L-Lysine Hydrobromide solution was applied in a Petri .