Complete inhibition of anisomycin and UV radiation but not cytokine induced JNK and p38 activation by an aryl-substituted dihydropyrrolopyrazole quinoline and mixed lineage kinase 7 small interfering RNA

..Our findings support the hypothesis that WRN could be involved in the correct resolution of recombinational intermediates that arise from replication arrest due to either DNA damage or replication fork collapse...

Complete inhibition of anisomycin and UV radiation but not cytokine induced JNK and p38 activation by an aryl-substituted dihydropyrrolopyrazole quinoline and mixed lineage kinase 7 small interfering RNA

..The combination of biochemical studies on DSIF and genetic analysis of Spt4 and Spt5 in yeast, also in this issue, indicates that DSIF associates with RNA Pol II and regulates its processivity in vitro and in vivo...

..Taken together, these data indicate that the catalytic activity of SHP-2 is required to regulate the PI3K/Akt pathway and thus likely participates in anti-apoptotic signaling by suppressing caspase 3-mediated apoptosis...

Global profiling of DNA replication timing and efficiency reveals that efficient replication/firing occurs late during S-phase in S. pombe

..Current studies of genome-wide DNA replication profiles have focused on the DNA replication timing and origin location. However, the replication and/or firing efficiency of the individual origins on the genomic scale remain unclear...

..Together, these results suggest a relationship between BLM, ATR, and the RMN complex in the response to replication arrest, proposing a role for BLM protein and RMN complex in the resolution of stalled replication forks...

Dynamic changes in subnuclear NP95 location during the cell cycle and its spatial relationship with DNA replication foci

..The purpose of this study is to report complications related to mitomycin C use in the treatment of glottic and subglottic stenosis. Risk factors associated with these complications are identified...

Nicotine is a developmental neurotoxicant and neuroprotectant: stage-selective inhibition of DNA synthesis coincident with shielding from effects of chlorpyrifos

..This study gives promising findings on the efficacy and safety of mitomycin-C as an adjuvant treatment in the management of selected cases of laryngeal and tracheal stenosis...

Can mitomycin C really prevent airway stenosis?

Jong Lyel RohDepartment of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of KoreaLaryngoscope 116:440-5. 2006

..However, the efficacy of MMC is still debatable, and the potential complications of MMC use have been scarcely reported. We evaluated the efficacy and early complications of MMC in a wound model of the subglottis...

Mediator, not holoenzyme, is directly recruited to the heat shock promoter by HSF upon heat shock

..We sought to identify risk factors for and review our experience in the management of adult acquired laryngotracheal stenosis (LTS) at an academic urban medical center...

The role of mitomycin C in preventing synechia and stenosis after endoscopic sinus surgery

Vijay K AnandDepartment of Otorhinolaryngology Head and Neck Surgery, Weill Cornell Campus of New York Presbyterian Hospital, Weill Medical College of Cornell University, New York, New York, USAAm J Rhinol 18:311-4. 2004

..Mitomycin C (MMC) has been used successfully in other fields to decrease postoperative scar formation. We hypothesize that the topical application of MMC reduces the incidence of stenosis and synechia formation after ESS...

Cyclin E is stabilized in response to replication fork barriers leading to prolonged S phase arrest

..Repair at the G(1) stage is not affected in CdLS cells. Our studies indicate that CdLS cells have a reduced capacity to tolerate DNA damage, presumably as a result of reduced DNA repair through homologous recombination...

O HjertnerInstitute of Cancer Research and Molecular Biology, and the Section of Hematology, Institute of Environmental Medicine, Norwegian University of Science and Technology, Trondheim, NorwayBlood 97:516-22. 2001

..BMP-4 or analogues may be attractive therapeutic agents in MM because of possible beneficial effects on both tumor burden and bone disease...

..CONCLUSIONS: A wound healing model based on tracheal revascularization, isolation, and reimplantation was developed in rabbits. This model allowed us to study the healing of full-thickness mucosal defects inside the airway...

Mitomycin-C in the treatment of tracheal cicatrix after tracheal reconstruction

..The results indicate that the cell shape change caused by staurosporine correlates with the accumulation of p27kip1 and that staurosporine interferes with the p27kip1-specific proteolysis activity...

Ezrin and moesin co-localise with ICAM-1 in brain endothelial cells but are not directly associated

..This finding supports the idea that cellular responses to growth factors may be driven by cell-matrix interactions and stresses the role of neuroendocrine factors in prostate carcinoma progression...

IL-15 up-regulates iNOS expression and NO production by gingival epithelial cells

..The enhanced NO production by IL-15 was inhibited by AMT, an iNOS-specific inhibitor. These results suggest that IL-15 is a potent regulator of iNOS expression by HGEC and involved in innate immunity in the mucosal epithelium...

Activation of the mitochondrial caspase cascade in the absence of protein synthesis does not require c-Jun N-terminal kinase

..While the agonists evoke a store-mediated Ca2+ entry that is dependent on the actin cytoskeleton, thapsigargin apparently activates an additional mechanism, which is independent of the disruption of the cytoskeleton...

..The contributions of spreading, migration, and mitosis were investigated both numerically and experimentally by using cytochalasin D to inhibit cell motility and mitomycin C to inhibit proliferation...

..These data suggest that in endothelial cells NCX and SERCA function in series to remove about half of the free Ca(2+) from the cytosol, while PMCA contributes to the other half of the Ca(2+) removal process...

Distinct pathways in the over-expression of matrix metalloproteinases in human fibroblasts by relaxation of mechanical tension

..These results point to the potent regulation operated by the status of the cytoskeleton on the cell phenotype, and to distinct regulatory pathways involved in the control of different MMPs expression...

..These findings further demonstrate the importance of interplay between cytoskeleton and substrate interactions as a key modulator of morphological and proliferative cellular response in hESCs on nanotopography...

Acute modulation of the extent of apoB mRNA editing and the relative rates of syntheses of apoB48 and apoB100 in cultured rat hepatocytes by osmotic and other stress stimuli

A McCahillInstitute of Biomedical and Life Sciences, University of Glasgow, ScotlandMol Cell Biochem 208:77-87. 2000

....

Coordinated regulation of procollagens I and III and their post-translational enzymes by dissipation of mechanical tension in human dermal fibroblasts

..Due to the partial loss of its CARD, the p84 multimer was devoid of caspase-9 or other caspase activity. Thus, our data indicate that Apaf-1 cleavage causes the release of caspases from the apoptosome in the course of apoptosis...

..The results identify components of the cascade by which Ca(2+) waves act to regulate neurite extension...

A novel model system for characterization of phagosomal maturation, acidification, and intracellular collagen degradation in fibroblasts

P D AroraMedical Research Council Group in Periodontal Physiology, The Faculty of Dentistry, and the Faculty of Medicine, Division of Respirology, University of Toronto, Toronto M5S 3E8, Ontario, CanadaJ Biol Chem 275:35432-41. 2000

..These studies demonstrate that the cellular machinery required for intracellular collagen degradation in fibroblasts closely resembles the vacuolar system in macrophages...

Shared and unique roles of CAP23 and GAP43 in actin regulation, neurite outgrowth, and anatomical plasticity

..It is concluded that inflammatory insults specifically induce translocation of Fas to the beta-cell surface and that interference with cell surface Fas expression is a new strategy to improve beta-cell survival in inflamed islets...

Synergistic enhancement of apoptosis by DNA- and cytoskeleton-damaging agents: a basis for combination chemotherapy of cancer

..tamarugo prior to wounding, and the activity of acidic cell-wall peroxidases increased after wounding in P. tamarugo but not in P. chilensis, we conclude that P. tamarugo is more tolerant to wound stress than P. chilensis...

..Disruption of the collagen framework is suspected to contribute to their development. Involvement of altered DNA metabolism may play a role in the chondrocyte necrosis that occurs early in the specific sites...

..3.) Evaluate the success of the training program through both process and outcomes evaluation. (4.) Implement continuous quality improvement procedures that will enhance the PPOR components, curriculum, and overall program. ..

..3) To determine the relationship between the mechanical activity of myo1c and membrane dynamics. We will express myo1c mutants that have altered motor properties, and we will examine the effect of these mutants on membrane dynamics. ..

..The proposed studies represent a logical extension of our previous work and will advance the field by defining the molecular organization of the BMC during normal and abnormal fiber end migration. ..

..Finally a set of rules for design of more potent rifamycin-like antibiotics will be formulated to achieve the long-term goal of knowledge-based creation of the new generation of antibacterial drugs. ..

..Identification of a potential mechanism for glucocorticoid action in the treatment of NS would permit the development of more highly targeted and less toxic therapies for this very common form of kidney disease. ..