Plot of normalized calcein intensity versus time for single AT-1 cells at temperatures of 37 (control), 40, 45, 50, 55, 60, and 70°C. The time in minutes is plotted on the x axis and the normalized intensity is plotted on the y axis in logarithm scale. The normalized intensity at any given time is indicated by a filled circle (•). Isotherms are joined by solid lines (——). Error bars represent the standard error. Also shown by dashed line (– – –) is the normalized intensity, which corresponds to 70 percent of the original intensity.

Plot of membrane injury to cells measured by propidium iodide exclusion versus time for single cells at temperatures of 40, 45, 50, 55, and 60°C. The time in minutes is plotted on the x axis and the survival fraction is plotted on the y axis in logarithm scale. The survival fraction at any given time after one hour of incubation after injury is indicated by a filled circle (•), and the corresponding isotherms are joined by solid lines (——). The survival fraction at any given time after three hours of incubation after injury is indicated by an open circle (○), and the corresponding isotherms are joined by dotted lines ([[dotted_line]]). Error bars represent the standard error. Also shown by dashed line (– – –) is the survival fraction, which corresponds to 0.7.

Plot of survival fraction of cells measured by clonogenic assay versus time for single cells at temperatures of 40, 43, 45, 46, 47, 48, and 50°C. The time in minutes is plotted on the x axis and the survival fraction is plotted on the y axis. The normalized survival fraction at any given time is indicated by a filled circle (•). Isotherms are joined by solid lines (–). Error bars represent the standard error. Also shown by dashed line ([[dashed_line]]) is the survival fraction, which corresponds to 0.7.

Plot of the logarithm of time (seconds) versus 1000 times reciprocal of temperature (in degree Kelvin) to reach a specific injury of 30 percent for different assays. Filled circles (•) represent calcein data, open circles (○) represent the data for PI (incubation time of 3 hours), and open triangles (▵) represent the data points for clonogenic assay corresponding to the time taken for the specific parameter to drop to 30 percent of its initial value for various temperatures. The slope of the linear fit to these data points was used to compute the activation energies.

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