Inducible Single Promoter Expression Vector for shRNA

The inducible PiggyBac vector features the ultra-tight cumate switch combined with the EF1-CymR repressor-T2A-Puro cassette to establish stable cell lines. The downstream H1 promoter will robustly produce shRNAs cloned into the MCS. The expression can be switched on simply by adding cumate to the cells. The all-in-one single vector format offers superior control of induction with no background leakiness.