A method of investigating the formation of antibodies by isolated cells

Abstract

Fig. 1. Arrangement for manufacture of microins t ruments. From left to right: LATR-1 autotransformer, M B S 1 microscope, "vise" , "furnace". To study antibody formation k has been proposed to investigate the immunolog ica l reactions of isolated solitary cells [1, 4]. The principle is that soli tary cel ls of the lymphat ic system of the immunized an ima l are placed in a minute drop of nutri t ive fluid, and a study made of the rate of synthesis of antibodies by them. The chief advantages of the method are that the class of ce l l to which the isolated cei ls belong may be determined and the rate of formation of antibodies by a single ce l l may be measured.