Baculovirus Expression System

Higher Eukaryotic System

The baculovirus-insect cells system is widely used to produce recombinant proteins from a wide range of organisms. As opposed to E. coli expression system, it makes use of a higher eukaryotic cellular machinery and usually yields overexpressed proteins with proper folding and disulfide bonding. Cellular localization is usually identical to that of native protein and most post-translational modifications found in higher organisms have been shown to occur usually at the same locations. The latter cellular processings include signal peptide cleavage, N- and O-glycosilation, phosphorylation, acetylation, amidation, etc. The baculovirus system has proven to be one of the most successful aproach to produce milligram amounts of recombinant structurally- identical to the native counterpart and most often biologically active proteins.

Standard Service Description

All services include multiple steps from gene to purified protein as described in the table below.