Join Life Technologies at the American Association of Clinical Chemistry Annual Meeting July 24–28 in Atlanta, GA

Visit us at booth#919 to see the latest molecular solutions from the Life Technologies portfolio of products, including innovative tools from Applied Biosystems®, Invitrogen®, Molecular Probes®, AcroMetrix®, and Ion Torrent™.

Stop by booth #919 for additional information on all of these products, plus a free gift (while supplies last) and the opportunity to win a Apple iPad 2.*

The technologies represented by these products include:

Nucleic acid purification

Sample preparation

Sequencing

Real-time PCR/qPCR

Controls and validation

Additional product brands presented at the Life Technologies booth include:

TaqMan® reagents

Applied Biosystems® 3500 Genetic Analyzer

ViiA™ 7 Real-Time PCR System

Veriti® Thermal Cycler

Applied Biosystems® 7500 Genetic Analyzer

iPrep™ Purification Instrument

Featured Workshop

Next-Generation Molecular Techniques

Attend our workshop and get information on new molecular techniques and platforms. These molecular methods are providing deeper insights in the areas of oncology, pharmacogenomics, infectious disease, and pathogen detection research. Applications and examples of these methods will be presented, demonstrating how these techniques have the potential to provide improved insight and utility.

Outbreaks of food-borne illness from pathogens such as E. coli, Listeria, and salmonella can represent significant public-health threats, with thousands sickened and a sizeable risk of patient death. more

Rapid and complete characterization of the microorganisms and strain involved, along with the development of tools to quickly screen suspect samples, are critical components of the public-health response. Ion Torrent has developed a novel, semiconductor-based, massively parallel (a.k.a. next-generation) sequencing system that is able to sequence whole microbial genomes in around 2 hours. This unprecedented speed, along with the system’s low cost, ease of use in comparison to other next-generation sequencing technologies, and scalability make it ideal for rapid sequencing of such microorganisms. An overview of the technology and workflow of semiconductor sequencing will be given, along with a real-world case study of how Life Technologies tools, including the Personal Genome Machine™ (PGM™) sequencer, capillary electrophoresis for multi-locus sequence typing, real-time quantitative PCR, and TaqMan® assays, were used to identify, characterize, and develop research screening assays in just one week for the novel E. coli 0104:h5 strain involved the German outbreak of May/June 2011.

Since the mapping of the human genome, the sequencing community continues to make the association between genes and disease, while at the same time realizing the disease research applicability of capillary electrophoresis (CE) for genotyping and sequencing. more

This applicability has been enhanced with the release of the BigDye® Direct Cycle Sequencing Kit, which represents a significant improvement to the current cycle sequencing workflow offering fewer steps, less handling time, and less hands-on time. This talk will focus on a specific example of how sequencing is being used to explore BRAF and KIT mutations in melanoma research samples.

Power of castPCR™ TaqMan® Mutation Detection Assays and Their Applications in Cancer Research Yun Bao, PhD, Staff Scientist, Molecular Biology, Life Technologies

The discovery of pivotal genetic alterations and the understanding of their potential roles in cancer are leading to remarkable successes in cancer research. more

Molecular methods such as DNA sequencing and conventional genotyping of tumor biopsies have advanced research in this field, but are limited in sensitivity due to stromal contamination and genetic heterogeneity in cancer. We have recently developed highly sensitive TaqMan® Mutation Detection Assays using our competitive, allele-specific TaqMan® PCR (castPCR™) technology for detecting and quantifying cancer-associated sequence variations in research samples. castPCR™ technology not only maintains the wide dynamic range, high sensitivity, and reproducibility of TaqMan® assays, but also greatly improves their specificity. This new technology enables detection of as little as 1 copy of mutant allele in 10 million copies of wild-type allele background.

Featured Products

New technologies presented at the Life Technologies booth include:

Ion Torrent Personal Genome Machine™ (PGM™) sequencer — perform a sequencing run in approximately two hours with this revolutionary, semiconductor-based benchtop instrument

*No purchase necessary to enter or win. Participation in this promotion is open only to registered attendees (not vendors) of AACC 2011 21 years and older. One entry per participant. Other eligibility restrictions apply. Odds of winning depend on the number of entries received. Your entry must be received by Life Technologies no later than 2 p.m. EST on July 28, 2011. One 16GB Apple iPad with Wi-Fi only (retail value $499) will be awarded. Winners must certify that they are not prohibited by employment, contract, or law from entering or accepting a prize from Life Technologies. Void where prohibited, licensed or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. A complete set of rules is available at Booth #919 .

Join Life Technologies at the American Association of Clinical Chemistry Annual Meeting July 24–28 in Atlanta, GA

Visit us at booth#919 to see the latest molecular solutions from the Life Technologies portfolio of products, including innovative tools from Applied Biosystems®, Invitrogen®, Molecular Probes®, AcroMetrix®, and Ion Torrent™.