18s rRNA Sequencing

Currently, 18s rRNA Sequencing study is getting more popular to define Fungi and Algae species by Using Sequence information. We Yaazh Xenomics, usually sequences the 18s rRNA ITS region to identify the fungi and algae. We usually use the ITS1/ITS4 primer for amplifying and sequencing of ITS region. If not amplifying in the above said primers we use ITS2, ITS3 and ITS5 primer combinations. With this ITS sequence data we can find out the similarity of given microorganism with various gene bank NCBI, EMBL, DDBJ, PDB sequence database and calculate the statistical similarity of matches between rRNA databases and target fungus or algae.

We do 18s rRNA sequencing for Fungi or Yeast or Algal identification; selective 18S rRNA gene could be sequenced using the common ITS universal primers. For ~650 bps sequences proper combined contigs would be provided.