Induction of calmodulin kinase IV by the thyroid hormone during the development of rat brain.

Details

Induction of calmodulin kinase IV by the thyroid hormone during the development of rat brain.

Journal

Journal of Biological Chemistry

Author(s)

KrebsJ., MeansR.L., HoneggerP.

ISSN

0021-9258 (Print)

ISSN-L

0021-9258

Publication state

Published

Issued date

1996

Volume

271

Number

19

Pages

11055-11058

Language

english

Abstract

This communication reports the specific induction of calmodulin kinase IV by the thyroid hormone 3,3',5-triiodo-L-thyronine (T3) in a time- and concentration-dependent manner at a very early stage of brain differentiation using a fetal rat telencephalon primary cell culture system, which can grow and differentiate under chemically defined conditions. The induction of the enzyme that can be observed both on the mRNA and on the protein level is T3-specific, i.e. it cannot be induced by retinoic acid or reverse T3, and can be inhibited on both the transcriptional and the translational level by adding to the culture medium actinomycin D or cycloheximide, respectively. The earliest detection of calmodulin kinase IV in the fetal brain tissue of the rat is at days E16/E17, both on the mRNA as well as on the protein level. This is the first report in which a second messenger-dependent kinase involved in the control of cell regulatory processes is itself controlled by a primary messenger, the thyroid hormone.