FAQ

We recommend amplification from the original starting material. If a large amount of amplified DNA will be needed, we recommend to increase the REPLI-g Kit reaction volume accordingly. Since the yield is determined by the reaction components, the final concentration of amplified DNA will be the same. Therefore, increasing the volume will increase the yield. However, if the original starting material is unavailable, the REPLI-g amplified DNA can be re-amplified at least once. We have not validated more than one round of re-amplification.