electroporator

Hello Dima et al.,
I've just purchased a BTX ECM 630. I haven't even unpacked
it yet. And I'm not yet familiar with the details of electroporation.
Do you know how that model compares to either the
Eppi or the BioRad? I know that they also make a more
full featured model (830 I think) that they claim can be used
even in 'in vitro' fertilization e-poration. And they have pretty
extensive/detailed protocols available online
http://www.btxonline.com/btx/index.htmlhttp://www.gentronics.com
Thank you,
Brad Turner [no affiliation, just HOPE to be a satisfied customer]
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Bradley Turner
Beth Israel Deaconess
Medical Center
Harvard Medical School 617-667-1215 phone
Division of Gastroenterology 617-667-2767 fax
Room Dana 605 bsturner at biosun.harvard.edu
330 Brookline Avenue bturner at caregroup.harvard.edu
Boston, MA 02215 bsturner at mbcrr.harvard.edu
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On Wed, 29 Nov 2000, Dima Klenchin wrote:
> elisa may <elisa.may at uni-konstanz.de> wrote:
> :Hi all,
> :has someone experience with the new electroporator from Eppendorf
> :(Multiporator)? We have no experience with electroporation yet and I´m
> :trying to figure out whether to buy Biorad or Eppendorf. I would like to
> :use it for bacteria and mammalian cells.
>> I looked up specs for Eppy machine and it appears to be a joke as
> far as mammalian cells transfection goes:
>> Voltage range Time constant
> Eukaryotic module
> 20-1,200 V
> 15-500 µs
> Bacteria module
> 200-2,500 V
> 5 ms
> Fusion module
> 5-300 V
> 5-500 µs
>> They really need to put bigger capacitors in there to
> allow for low voltage long duration pulses with tau in the
> range of 10-50 ms.
>> Also, it appears that with high voltage applications (bacteria,
> yeasts) you are stuck with only one shunt resistance,
> which is really inflexible and cheap. IMHO, Bio-Rad is
> unquestionably better.
>> - Dima
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