Dear Netters!
I'm trying to check some yeast transformants by transforming E. coli with
their DNA. The trouble is that I don't get any transformant of E. coli.
How can I prepare the yeast DNA to get some transformants?
It's important to quantify the DNA? If so, which is the optimal
concentration?
Is there any trick to improve the transformation?
If someone can help me, please send the answer to my personal account. I
will post a summary of the answers I receive.
Thanks a lot,
Esther Guarinos
Centro de Biologia Molecular "Severo Ochoa"
Universidad Autonoma de Madrid-CSIC
Cantoblanco. 28049 Madrid
SPAIN
Phone: 34-1-3975077
Fax : 34-1-3974799
e-mail: eguarinos at mvax.cbm.uam.es