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CellTiter-Glo® 2.0 Measures ATP, a Key Indicator of Cell Health

CellTiter-Glo® 2.0, based on the original CellTiter-Glo® Assay chemistry, is a single-reagent format with improved stability for convenient storage and simpler setup. Whether you perform assays in a single plate or process hundreds of plates at a time, the ready-to-use reagent is designed for fast and easy everyday use. A highly sensitive assay with broad linearity, it is amenable to high throughput screening and multiplex applications.

The CellTiter-Glo® 2.0 Assay determines the number of viable cells in culture by quantifying ATP, which indicates the presence of metabolically active cells. Luminesence readout is directly proportional to the number of viable cells in culture.

Simple Assay Setup and Enhanced Stability

The CellTiter-Glo® 2.0 reagent is stable at room temperature and at 4°C, allowing fast and easy assay implementation, and eliminating the thawing and mixing requiring with other ATP-based assays. Enhanced stability also allows convenient storage.

Percent Activity Remaining

CellTiter-Glo® 2.0 Reagent Stability

CellTiter-Glo® "Original" Reagent Stability

>85% Activity at 22°C

7 days

12 hours

>85% Activity at 4°C

2 months

3.5 days

Assay Performance

The CellTiter-Glo® 2.0 Viability Assay exhibits the same high performance as the classic CellTiter-Glo® Assay, making it suitable for use from benchtop scale to high throughput screening.

High sensitivity and broad linearity. Luminescence measured with the CellTiter-Glo® 2.0 Assay is proportional to the number of viable Jurkat cells in culture over three orders of magnitude in 96-well, 384-well and 1536-well plates.

Multiplex Capability

CellTiter-Glo® 2.0 Assay can be multiplexed with other assays, reducing cell culture costs and labor. Multiplexing assays in the same sample well allows complementary measures of cell health to correct for errors in culturing, plating or assay chemistry interferences.