Figure 4: Light scattering by intracellular gas vesicles. Vacuolated cells were centrifuged at 5000 xg for 5 mins to deflate gas vesicles. OD600 and CFU counts were taken before and after centrifugation. No decrease in CFU or change in morphology were observed before and after centrifugation meaning that difference in OD600 can be directly attributed to gas vesicle loss. Error bars represent +/− one standard deviation of the quadruplicate measurements. OD600 values are included below each column as reference.