Overview

PSD95 has a predicted band size of 95kDa, however in western blot gels it tends to run at a larger band size (~105kDa), this is thought to be due to post-translational modification (possibly palmitoylation) of PSD95 proteins.

Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

IHC-FoFr

1/300.

IP

Use at an assay dependent concentration.

WB

Use a concentration of 0.3 - 3 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 95 kDa).

Target

Function

Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.