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In vitro Fertilization

In vitro fertilization is the most commonly used technique in order to recover a mouse line from frozen sperm.

It can also be performed with fresh sperm in order to generate a high number of 2-cell embryos which can be either cryo-preserved or transferred to a recipient mother. It can help to speed up the re-derivation process of a mouse line or to quickly expand a mouse colony, generating a big number of pups born at the same time. In these cases, frozen sperm can be used, but also a sample of fresh sperm can be taken from the male keeping him alive.

You would provide us with a male that has been previously proved to be fertile. By default, we would fertilize C57Bl/6N oocytes, but other genetic backgrounds can be considered upon request.