Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

ICC/IF

Immunomicroscopy

Flow Cyt

ELISA

IHC-P

IHC-Fr

Application notesSuitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency
For FACS analysis, it is useful to do titration for this secondary antibody: 1:1000, 1:2500 and 1:5000 and the blocking buffer should be 3% BSA.

References for Rabbit Anti-Goat IgG H&L (FITC) preadsorbed (ab7128)

ab7128
has not yet been referenced specifically in any publications.

Publishing research using ab7128? Please let us know so that we can cite the reference in this datasheet.