应用

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用

Ab评论

说明

Flow Cyt

Use a concentration of 2 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF

1/1000.

靶标

功能Receptor for interleukin-2.

疾病相关Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.

Anti-IL2 Receptor alpha antibody [MEM-181] 图像

Flow Cytometry - Anti-IL2 Receptor alpha antibody [MEM-181] (ab8235)

Overlay histogram showing Jurkat cells stained with ab8235 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8235, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed Jurkat cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

The antibody ab8232 is unconjugated and so you would need to use a secondary antibody for detection. If you tell me what techniques you are using I would be happy to make some suggestions about suitable secondary antibodies.
Please let me know if ther...

I can confirm that this product does not required pemeabilisation when it was tested in FACS as the recognized epitope is extracellular.
Please do not hesitate to contact us if you require further information.

Thank you for your enquiry. I ran a search for a neutralising antibody directed against the IL2 Receptor alpha and unfortunately could find any results. This does not mean that the ab8235 will not work in blocking IL2, it just means that we have not te...

Thank you for your message.
We are very sorry but there was a discrepancy on our datasheet. We would like to confirm that this antibody has not been tested and characterised for Western blot application.
Thank you for your feedback; it is very ...

Our datasheet currently states that the antibody has not yet been tested in WB and I apologise if it previously did; I have checked with our source and they confirmed they have not tested it yet.
Should you wish a refund can you please give me your...