Quantitative Determination of Peptides by Sulfhydryl (-SH) Groups(Contributed by David Van Horn, Dept. of Chemistry, UC Berkeley Greg Bulaj, Dept. of Biology, University of Utah)This is the standard 揈llman抯 Test?for the determination of free thiols. It works well for small peptides and proteins synthesized using standard solid phase synthetic methods. Added: Tue May 14 2002, Reviews: 0Write review

Semi-Quantitative Measurement of Proteins by Dot Blotting
(Springer Lab, Harvard University)Concentration of proteins in a crude preparations (such as culture sup) can be estimated semi-quantitatively by using "Dot Blot" method if you have both purified protein and specific antibody against it.http://labs.idi.harvard.edu/springer/protocols/jun...Added: Sun Oct 25 2009, Hits: 334, Reviews: 0Write reviewCached

UV Absorbance Protein Determination
(Dr. Mario Lebendiker, The Hebrew University of Jerusalem)The advantages of this method are that it is simple, and the sample is recoverable. The method has some disadvantages, including interference from other chromophores, and the specific absorption value for a given protein must be determined. http://wolfson.huji.ac.il/purification/Protocols/O...Added: Tue Feb 17 2009, Hits: 1524, Reviews: 0Write reviewCached