The Qproteome Mammalian Protein Prep Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Efficient extraction of proteins from cultured cells.

Duplicate samples (each 5 x 106 cells) from the indicated cell lines were processed using the Qproteome Mammalian Protein Prep Kit. Proteins in the supernatants (S) and pellets (dissolved in 4% SDS, P) were separated by SDS-PAGE. The presence and location of the indicated proteins was analyzed by probing western blots with the relevant protein-specific antibody and chemiluminescent detection. M: markers.

The Qproteome Mammalian Protein Prep Kit provides gentle but efficient detergent-based lysis of mammalian cells to deliver a soluble protein fraction suitable for any downstream application. Cell pellets are incubated with Mammalian Cell Lysis Buffer supplemented with Benzonase — an enzyme that digests nucleic acids — and a protease inhibitor, which protects the released proteins from enzymatic degradation. No specialized equipment is required.

Procedure

Cells are simply incubated with lysis buffer and centrifuged to separate cell debris. The supernatant contains the soluble protein fraction, which can be further purified or directly analyzed, for example by SDS-PAGE.

Applications

The Qproteome Mammalian Protein Prep Kit is used for total protein preparations from mammalian cells for proteomics procedures (e.g., 2D-PAGE, mass spectrometry).