ELISA based Qualitative immuno blot test *Auto immune antibodies to the following nuclear antigens can be detected under this test.

NRNP/Smith

Jo-1

Smith

Centromeres

SS-A(Ro)

dsDNA

Ribosomal P – Protein

Nucleosomes

SS-B(La)

Histones

Scleroderma-70

AMA-M2

PM-Scl

***

Clinical Significance

Anti UI-nRNP High titres present in 95%-100% of patients with mixed connective tissue disease, Sharp syndrome.The titre correlates with the clinical activity of the disease.Also found in 30-40% of patients with disseminated lupus in combination with anti smith.

Anti-Smith Highly specific and present in 20%-40% of patients with disseminated lupus .

Anti SS-A Present in patients with Sjogren’s Syndrome (40%-80%), disseminated lupus(30-40%), primary billary cirrosis (20%), neonatal lupus (100%), occationally in chronic active hepatitis. New born babies some times may get congenital heart block because of anti SS-A when they are transmitted to the foetus from mother.

Anti SS-B Found in Sjogren’s syndrome(40-80%)mostly in women, disseminated lupus(10-20%).

Anti Scl-70 Found in diffuse progressive systemic sclerosis(25%-75%).

Anti PM-Scl Found in 50-70% of patien’s with overlap syndrome.

Anti Jo-1 Found in polymyositis(25%-35%),mostly associated with a concurrent interstitial fibrosis of the lung.