Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

ELISA

Use at an assay dependent concentration.

IHC-Fr

Use at an assay dependent concentration.

ICC/IF

1/150.

IHC-P

Use at an assay dependent concentration.

WB

1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 26.6 kDa).

IP

Use a concentration of 10 µg/ml.

Target

FunctionDestroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

Involvement in diseaseGenetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.

ab13533 staining Superoxide Dismutase 2 in Human normal fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% BSA for 1 hour at 37°C. Samples were incubated with primary antibody (1/150 in 1% BSA) for 2 hours at 37°C. An undiluted Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody. Nuclei were counterstained with DAPI.

ab13533 staining Superoxide Dismutase 2 in human Hella cells by ICC/IF. Cells were acetone fixed and blocked in 5% serum for 1 hour at 24°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 24°C. The secondary antibody used was Alexa Fluor®594 donkey anti-rabbit diluted at 1/100.

ab13533 staining NGF-differentiated PC12 cells (rat) by ICC/IF. Cells were PFA fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 10% serum for 1 hour at 25°C. The primary antibody was diluted to 2µg/ml in PBS and incubated with the sample for 1 hour at 25°C. An Alexa Fluor® 488 conjugated goat anti-rabbit antibody was used as the secondary.