(Optional) To assure that the original signal is removed, wash membrane twice for 5 minutes each with 10 ml of TBS/T. Incubate membrane with LumiGLO® with gentle agitation for 1 minute at room temperature. Drain membrane of excess developing solution. Do not let dry. Wrap in plastic wrap and expose to x-ray film.

Wash membrane again four times for 5 minutes each in TBS/T.

The membrane is now ready to reuse. Start detection at the “Membrane Blocking and Antibody Incubations” step in the Western Immunoblotting Protocol.