ØAfter completion of the analysis, follow the reverse procedure to convert to reverse phase.ØDo not store system in solvents.Ø For purging of system start with the lower flow and then stabilize system at the required condition.ØAfter analysis take backup of the data in the relevant location.

ØEnsure that the system is calibrated before use.

ØBefore the start, ensure that respective solvents & inlets inserted are as per the requirements.

ØAfter analysis, do not leave the system idle in harsh solvents like ACN, store the system in ACN: Water mixture (20:80).