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Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Likely glioblastoma

Age

unknown

Gender

male

Ethnicity

unknown

Applications

This cell line is a suitable transfection host.

Storage Conditions

liquid nitrogen vapor phase

Karyotype

This is a hypodiploid human cell line with the modal chromosome number of 44 occurring in 48% of cells. The rate of higher ploidy was 5.9%., Twelve markers were common to all cells, including der(1)t(1;3) (p22;q21), der(16)t(1;16) (p22;p12), del(9) (p13) and nine others. The marker der(1) had two copies in most cells. There was only one copy of normal X. N1, N6 and N9 were not found.

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Derivation

This is one of a number of cell lines derived from malignant gliomas (see also ATCC®HTB-15™ and HTB-16™) by J. Ponten and associates from 1966 to 1969 (see also Allen, 2016).

Clinical Data

male

Tumorigenic

Yes

Effects

Yes, in nude mice inoculated subcutaneously with 10(7) cells

Comments

Mycoplasma contamination was eliminated in September 1975.

The ATCC® HTB-14™ cell line was deposited at ATCC in 1982.

STR profiling, Y-chromosome paint, and Q-band assay confirmed that the cell line is male in origin. Based on current literature, the cell line is likely a glioblastoma of CNS origin (Allen, 2016).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.