* The optimum coating concentration depends on cell lines from 0.1 to 1.5ug/cm².

** For example, when you use 6-well plate (9.6 cm²/well), add 9.6ul iMatrix-511 (4.8ug) in 1.99ml of PBS(-). Add 2mL of diluted iMatrix-511 solution to the well.

2. Incubate for 1 hour at 37C, 3 hours at Room temperature, or over night at 4C.

3. Remove excess fluid from the coated surface. No rinse is needed.

4. Immediately plate the cells at desired density.

*** Don't allow the plate to dry.

**** Briefly spin down all liquid in the tube before use.

***** Avoid repeated freeze-thaw cycles.

------------------------------------------------------------------------------------------------****When culturing ES/iPS cell, Feeder Free and Single cell subculture can be done using iMatrix-511.*****

*License to manufacture and sell this product has been obtained from Osaka University & Kyoto University.*