The Effect of Temperature On the Transmission of Tobacco Rattle Virus in Tulips By Trichodorus, Using the "Bait-Leaf" Method

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Certain cultivars of tulip are susceptible to tobacco rattle virus infection. The rate of infection is high when planted early, but decreases rapidly to zero with planting later in the season. Trichodorus infects mainly the roots with virus although infection of the sprouts occurs sporadically. The bait plant method is unsuitable for measuring the activity of thhe nematodes at different temperatures because certain bait plants such as White Burley tobacco do not grow at all at low temperatures and others grow at such different rates at different temperatures that they cannot be compared. The "bait-leaf" method proved superior in assessing the activity of the nematodes with respect to virus transmission. Virus transmission at 4°C was extremely low, a maximum was reached at 15° and a decrease was noticed at 20°.