Immunogenomics

The adaptive immune system employs an immensely diverse repertoire of lymphocyte receptors to recognize and neutralize foreign agents. Analyzing the composition of B- and T-cell receptors in vivo can provide insight into diseases and aid the development of biologics and vaccines. Surface display technology can also be used as a complementary or alternative approach to discover antibodies, including both antigen-binding (Fab) and single-chain variable fragments (scFv), targeted against an antigen.

Service Details and Workflows

Immuno-Profiling

Perform bulk analysis of V(D)J sequences at the RNA level.

Short-Read Amplicon Sequencing

Assay the diversity of clones after bio-panning in surface display experiments. Heavy and light chains, amplified separately, are fully sequenced on the Illumina MiSeq platform. We offer the following NGS services for this approach:

Amplicon-EZ

A cost-effective solution that provides 50,000 reads per sample with a fast turnaround time.

TargetGxOne™

Single-Cell Immuno-Profiling

Long-Read Amplicon Sequencing

Analyze clonal diversity after bio-panning via long-read sequencing technology. Heavy and light chains amplified together in a single PCR product are contiguously sequenced using the PacBio Sequel® platform. This approach physically pairs the sequences of the two chains.