Protocols

Specificity / Sensitivity

PDLIM2 Antibody recognizes endogenous levels of total PDLIM2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly255 of human PDLIM2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from various cell lines and mouse lung using PDLIM2 Antibody.

Western Blotting

Western blot anlysis of extracts from 293T cells, mock transfected (-) or transfected with a human PDLIM2 construct (hPDLIM2, +), using PDLIM2 Antibody.

Background

PDLIM2, also known as Mystique, contains an amino-terminal PDZ domain and a carboxy-terminal LIM domain. PDLIM2 was orginally found to be associated with cytoskeletal proteins in epithelial cells to promote cell attachment and migration (1,2). Subsequent studies have shown that PDLIM2 can also inhibit NF-κB activity by acting as a nuclear ubiquitin E3 ligase for p65 (3). PDLIM2 is suppressed in cancer cell lines by DNA methylation (4,5). Expression of PDLIM2 can inhibit anchorage-independent growth and tumor formation.