Determination of Telomeric Protein Interaction Binding Sites Between Amino Acids 58-268 of TRF1 and 224-276 of TIN2 and Between Amino Acids 42-245 of TRF2 and 203-361 of TIN2

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Abstract

Telomerase:
• A ribonucleoprotein complex, with RNA and protein components, in all eukaryotes
• Required for lengthening the ends of telomeric DNA by reverse transcriptase
Without Telomerase:
• Unable to lengthen chromosomes, DNA shortens with each replication
• Results in the loss of genetic information and greater susceptibility to mutation
Unknown:
• The exact amino acid interactions of the telomeric proteins
• The nature of the interaction between certain telomeric proteins
The Study will:
• Examine the relations of three telomeric proteins, TRF1, TRF2, and TIN2
• Attempt to form complexes with protein fragments of decreasing size (Figure 1)
• Examine the interaction between TRF1 and TRF2
• Lead to greater understanding concerning telomeric protein binding

Interactions of telomeric protein-protein complexes have important implications in the
development of diseases such as cancer. Approximately 90% of all cancer cases are associated
with the reactivation of telomerase ...

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