Figure 2

Circumvention of doxorubicin-retention by cyclosporin-A (Cs-A). INER-51 cells growth as monolayers (filled circles) or multicellular spheroids (open circles) were treated with increase concentrations of Cs-A for 1 hour previous to being loaded with 30 μM of DXR for 30 min in presence of reversal agent. Doxorubicin retained was determinate by spectrofluormetry. Each point represents the mean of at least 3 experiments and the error bars are the standard error of the mean.