Cytofluorometry, Flow

Cytometries, Flow

Cytometry, Flow

Flow Cytofluorometries

Flow Cytofluorometry

Flow Cytometries

Flow Microfluorimetry

Flow Microfluorometries

Flow Microfluorometry

Fluorescence Activated Cell Sorting

Fluorescence-Activated Cell Sorting

Fluorescence-Activated Cell Sortings

Microfluorimetry, Flow

Microfluorometries, Flow

Microfluorometry, Flow

Sorting, Fluorescence-Activated Cell

Sortings, Fluorescence-Activated Cell

Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercuryarc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include lightabsorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.