Mutation details: Missense mutations were introduced at codons 125 and 126 (exon 3), resulting in Glu to Gly and Gly to Ser substitutions. The substitutions were within the P-box of the first zinc finger and were shown, in vitro, to abolish DNA-binding while retaining the ability to interact with T3 and cofactors. The mutations were verified by sequencing RT-PCR products. The self-excision of a neo cassette from intron 3 was confirmed, leaving a single loxP site. Western blot analysis showed endogenous levels of protein in homozygous mutant mice.
(J:85158)