The pRNAi vector system is designed to facilitate the cloning of the double-strand DNA oligonucleotide encoding a stem-loop sequence, which is transcribed into shRNA by RNA polymerase III promoter (e.g. H1, human and mouse U6 promoters) once expressed in cells. Our single oligonucleotide RNAi technology (SORT) for gene silence delivers a unique feature in the construction of shRNA expression vectors. Distinct from any other shRNA expression vectors currently available, only one DNA oligonucleotide is required for cloning a shRNA sequence into the pRNAi vector. The novelty of our pRNAi vector system benefits users with lower cost, higher efficiency, and reduced complexity.

FavorPrep Plasmid DNA Extraction Mini Kit (100)

Predesigned shRNA Vector Kits w/ Optional Lentivirus

Biosettia designs and clones four gene-specific shRNA into any one of our vectors, perfect for specific, long-lasting gene silencing. At least one shRNA will knock down its target by at least 70% at the RNA level as measured by real time qRT-PCR. Each kit also includes two negative controls.

Cat#: SORT-B35

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