The spec file is the description of what you want PyInstaller to do
with your program. pyi-makespec is a simple wizard to create spec
files that cover basic usages:

py-Makespec [--onefile] yourprogram.py

By default, pyi-makespec generates a spec file that tells
PyInstaller to create a distribution directory contains the main
executable and the dynamic libraries. The option --onefile
specifies that you want PyInstaller to build a single file with
everything inside.

In most cases the specfile generated by pyi-makespec is all you
need. If not, see When things go wrong in the manual and be sure to
read the introduction to Spec Files.

A path to search for imports (like using PYTHONPATH).
Multiple paths are allowed, separated by ‘:’, or use
this option multiple times

--hidden-import MODULENAME, --hiddenimport MODULENAME

Name an import not visible in the code of the
script(s). This option can be used multiple times.

--additional-hooks-dir HOOKSPATH

An additional path to search for hooks. This option
can be used multiple times.

--runtime-hook RUNTIME_HOOKS

Path to a custom runtime hook file. A runtime hook is
code that is bundled with the executable and is
executed before any other code or module to set up
special features of the runtime environment. This
option can be used multiple times.

--exclude-module EXCLUDES

Optional module or package (his Python names, not path
names) that will be ignored (as though it was not
found). This option can be used multiple times.

Add or update a resource to a Windows executable. The
RESOURCE is one to four items,
FILE[,TYPE[,NAME[,LANGUAGE]]]. FILE can be a data file
or an exe/dll. For data files, at least TYPE and NAME
must be specified. LANGUAGE defaults to 0 or may be
specified as wildcard * to update all resources of the
given TYPE and NAME. For exe/dll files, all resources
from FILE will be added/updated to the final
executable if TYPE, NAME and LANGUAGE are omitted or
specified as wildcard *.This option can be used
multiple times.

--uac-admin

Using this option creates a Manifest which will
request elevation upon application restart.

--uac-uiaccess

Using this option allows an elevated application to
work with Remote Desktop.

Any Shared Assemblies bundled into the application
will be changed into Private Assemblies. This means
the exact versions of these assemblies will always be
used, and any newer versions installed on user
machines at the system level will be ignored.

--win-no-prefer-redirects

While searching for Shared or Private Assemblies to
bundle into the application, PyInstaller will prefer
not to follow policies that redirect to newer
versions, and will try to bundle the exact versions of
the assembly.

Mac OS X .app bundle identifier is used as the default
unique program name for code signing purposes. The
usual form is a hierarchical name in reverse DNS
notation. For example:
com.mycompany.department.appname (default: first
script’s basename)