... In this study, the antimicrobial resistance profiles of L. monocytogenes isolated from chicken meat in Fukuoka in 2017 were compared with the isolates of 2012. A total of 85 and 50 chicken meat samples, including different body parts, were collected from different supermarkets in Fukuoka in 2012 and 2017, respectively. Detection, isolation, identification, and characterization of L. monocytogenes ...

... The aim of the study was to determine antimicrobial resistance and genotypic characteristics of L. monocytogenes isolated from food of animal origin from different parts of Poland during years 2013–2016. A total of 146 isolates were tested using a microbroth dilution method, whereas virulence genes and molecular serogroups were identified by PCR. Pulsed-field gel electrophoresis (PFGE) and multilo ...

... Listeria monocytogenes serotype 1/2a is considered as a foodborne pathogen, resulting in high morbidity. Detection of Listeria monocytogenes serotype 1/2a is rather difficult and time-consuming thus, the need for better detection methods is encouraged. In order to rapidly detect and identify L. monocytogenes serotype 1/2a, specific target genes were mined by the comparative genomics approach. Thre ...

... Listeria monocytogenes is a relevant pathogen usually associated with meat and ready-to-eat products. This study aimed to assess the distribution, adhesion, virulence and antibiotic resistance of L. monocytogenes in a pork production chain. Environment, carcass and food samples (n = 894) were obtained from different steps of a pork production chain over a 6-month period (10 samplings), including f ...

... A trial was conducted to determine the genotypic and phenotypic profiles of Listeria monocytogenes (LM) isolated from chicken meat and chicken-meat processing environment in an export-authorized industrial facility in Brazil. A total of 37 LM isolates were separated according to their genetic similarity by pulsed-field gel electrophoresis (PFGE), classified by serotype and lineage using multiplex ...

... In this study, of 124 samples of ground beef and chicken meat, 101 (81.5%) were positive for Listeria spp. Listeria innocua (35.5%) and Listeria monocytogenes (26.6%) were the most frequently isolated species. All 33 (100%) L. monocytogenes isolates were positive for the virulence genes hlyA, actA, inlA, inlB, inlC, inlJ, prfA, plcA, and iap. Serotype 1/2a, which is one of the listeriosis‐associat ...

... The aim of this research was to investigate the occurrence of Listeria monocytogenes in fish and fish processing plant and to determine their transmission, virulence and antibiotic resistance.L. monocytogenes was isolated according to the ISO 11290–1. The identification of L. monocytogenes was confirmed by multiplex PCR method. Genetic similarity of L. monocytogenes strains was determined with the ...

... AIMS: The aims of this study were to characterize the genetic diversity of Listeria monocytogenes isolates obtained from commercial mushroom production, to establish the persistence, recontamination and the risk of cross‐contamination from the working environment to the final products, creating awareness about the presence of L. monocytogenes thus helping to prevent the possibility of cross‐contam ...

... A total of 1096 food samples were purchased in Tetouan, North-Western of Morocco, to examine the presence of Listeria spp. Eighty (7.3%) of the tested samples were found positive for the presence of Listeria spp., while L. monocytogenes was detected in 16 (1.5%) samples. L. monocytogenes isolates belonged to clonal complexes CC2 (PCR serogroup IVb) and CC199 (PCR serogroup IIa) and to 8 different ...

... Hunting is currently a very popular activity, and interest in game meat is increasing. However, only limited research is available on the bacterial quality and safety of moose (Alces alces) and white-tailed deer (Odocoileus virginianus) harvested by hunters. Poor hunting hygiene can spread bacteria onto the carcasses, and inadequate chilling of the carcasses may increase the bacterial load on the ...

... The present study aimed to determine the prevalence of Listeria spp. in stray dogs in the Kayseri province of Turkey. In addition, serotyping, genotyping and virulence gene analysis of the isolated Listeria spp. were performed and their pathogenicity and antibacterial susceptibility were investigated.The study included 80 rectal swaps taken from 80 stray dogs of different ages and gender that were ...

... Listeria monocytogenes is a foodborne pathogen worldwide. Multilocus variable-number tandem repeat analysis (MLVA) has been used for listeriosis surveillance and outbreak investigations. MLVA typing schemes have been proposed, but their usefulness for typing isolates from the People's Republic of China has not been assessed. To this aim, all L. monocytogenes strains (79) isolated from 1,445 raw me ...

... Listeria monocytogenes is a serious foodborne pathogen capable of persisting in food processing environments. Tolerance to disinfectants used in industrial settings constitutes an important factor of Listeria survival. In the present study, the mechanism of tolerance to benzalkonium chloride (BAC) was investigated in 77 L. monocytogenes isolates from a meat facility. By PCR approach, the mdrL and ...

... A total of 47 Listeria monocytogenes isolates were separated and identified from 153 retailed raw meat samples in Shanghai area with the highest contamination rate in pork meat (20.34%). Using multiplex PCR, these isolates were divided into 3 serogroups: 1/2a‐3a, 1/2b‐3b‐7, and 1/2c‐3c, and 1/2a‐3a was predominant in these isolates. Calgary device was introduced to cultivate biofilm, and crystal v ...

... Listeria monocytogenes is a foodborne pathogen causing listeriosis, which potentially affects all individuals, especially pregnant women and immunocompromised persons. The present study investigated the prevalence, antimicrobial susceptibility and serotypes distribution of the isolated L. monocytogenes from Iran. Twenty two (4.97%) of 442 human, food and livestock samples were found to be positive ...

... AIMS: Using molecular subtyping techniques, Listeria monocytogenes is divided into three major phylogenetic lineages, and a multiplex PCR method can differentiate five L. monocytogenes subgroups: 1/2a‐3a, 1/2c‐3c, 1/2b‐3b‐7, 4b‐4d‐4e and 4a‐4c. In this study, we conducted genome comparisons and evaluated serotype‐associated genes for their utility as a multiplex PCR‐based method for distinguishing ...