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10,000 I.U./mL Penicillin, 10,000 (μg/mL) Streptomycin For reducing the chances of microbial contamination in cell culture. Between 0.5 and 1 mL of Penicillin-Streptomycin solution are added to 100 mL of cell culture media for a final concentration of 50 to 100 I.U./mL penicillin and 50 to 100 (μg/mL) streptomycin.

Comments

NOTE: Most cell culture technologists avoid using antibiotics and antimycotics for routine culture work. Antibiotics and antimycotics may mask contamination by susceptible bacteria and fungi while permitting mycoplasma to flourish unnoticed. Antibiotics and antimycotics may interfere with the metabolism of sensitive cells in culture. However, one may elect to introduce antibiotics and antimycotics for short periods to primary cultures or as a safeguard while propagating specific valuable stocks.

Biosafety Level

1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Quality Control Specifications

Lot-specific test results are listed in the Certificate of Analysis, which is available at www.atcc.org or by contacting ATCC Technical Service.

SPECIFICATION CERTIFICATE

Test

Specification

pH1

Report results

Osmolality2

Report results

Endotoxin3

≤ 0.25 EU/mL @ 1:100

Antimicrobial Activity4 (1:100)

Inhibition of S. aureus & E. coli

Sterility5

Pass

Measured with a standard pH meter. Meters are calibrated daily using standards traceable to the National Institute of Standards Technology using guidelines outlined in the current edition of USP.

Measured with a standardized osmometer, i.e. freezing point depression. Meters are calibrated daily using standards traceable to the National Institute of Standards Technology using guidelines outlined in the current edition of USP.