3f)onmatrixengagementcorrespondingwith signalling within the endosomes or the potential of integrins to set offdecreased integrin endocytosis observed in Rab21-depleted cells9.signalling from endomembranes. To review this more we isolatedOn top of that,adhesion-inducedsignalling(pFAKlevels)wassignif- Why HMG-CoA Reductase inhibitorGreatly improved Our Life Last Year endosomes from Fak+/+ and Fak?/? MEFs. ��1-integrin localizedicantlyimpairedincellsexpressinganendocytosis-defectivecollagen- to endosomal and plasma membrane fractions in the two cell stylesbinding integrin (��2-subunit KR1160/61AA mutation as compared indicatingthatintegrinendosomallocalizationisnotFAKdependentwithwild-type��2-integrinreceptor-expressingcellsadheringtocolla- (Fig.4a).Aswefoundthatendosomescontainasubsetofintegrin-28gen (Fig.

3g).Takentogether,thesedatademonstratethatimpaired signalling components (Fig. 2b) we up coming addressed the probabilityintegrinendocytosisattenuatesadhesion-inducedFAKactivation.that these parts could assemble directly around the endosome.NATURECELLBIOLOGY ADVANCEONLINEPUBLICATION5? 2015 Macmillan Publishers Limited. All rights reservedIrynaShnitsar1,MikhailBashkurov1,GlennR.Masson2,AbiodunA.Ogunjimi1,SherlyMosessian3,EduardoAguiarCabeza1,CalleyL.Hirsch6,DanielTrcka1,GeraldGish6,JingJiao3,HongWu3,RudolfWinklbauer4,RogerL.Williams2,LaurencePelletier1,5,JeffreyL.Wrana1,5&MiriamBarrios-Rodiles1Cilia are hair-like cellular protrusions important in many aspects of eukaryotic biology.