Archive for the ‘Sarcoidosis’ Category

Venet et al reported that in sarcoidosis, antigen-presenting capacity by AMs to lung T-lymphocytes was not significantly different from that to blood T-lymphocytes. In the present study, similar results were obtained. We not only confirmed their findings but also demonstrated that antigen-presenting capacity by monocytes to lung T-lymphocytes was not significantly different from that to […]

Both IL-1 and IFN-7, which have been reported to be increased in sarcoidosis, may have enhanced the antigen-presenting capacity in sarcoidosis, but both IL-1 and IFN-7 failed to induce antigen-presenting capacity in control subjects in the present study. Other possibilities for enhancing antigen-presenting capacity include a difference in the density of lymphocyte function associated antigens […]

Many cells other than T-lymphocytes are known to be able to function as APCs in humans, including Langerhans’ cells, astrocytes, and monocytes.’ We have demonstrated in the present study that monocytes in normal control subjects can function as APCs and their antigen-presenting capacity is not significantly different from patients with sarcoidosis. On the other hand, […]

HLArDR Antigens on the Surface of AMs and Monocytes It has been shown that an optimal response of sensitized T-lymphocytes to antigens requires the combined recognition of the nominal antigen plus HLA-DR antigens on the surface of APCs.* Thus the enhanced antigen-presenting capacity by AMs in patients with sarcoidosis may have been caused by the […]

Next, we examined the antigen-presenting capacity by AMs. As reported previously, AMs from control subjects demonstrated minimal ability to function as APCs. On the other hand, AMs from patients with sarcoidosis showed an antigen-presenting capacity to autologous T-lymphocytes which was optimal when the ratio of AMs to T-lymphocytes was 1:5 (data not shown). The antigen-presenting […]

Antigen-Presenting Capacity The antigen-presenting capacity by further purified AMs and monocytes with rosette formation was consistent with that by AMs and monocytes without further purification. Therefore, the following results were obtained by using AMs and monocytes without further purification.

The cultures were incubated for six days at 37°C in a humidified atmosphere of 5 percent carbon dioxide in air. To quantify T-lymphocyte proliferation, at 16 to 18 hours prior to harvesting, 0.2jtCi of tritiated thymidine was added to each well. Triplicate samples were harvested using a multiple sample harvester and counted in a liquid […]

T-lymphocytes were purified from BAL fluid and blood during the AM and monocyte purification procedure. Nonadherent cells from plastic culture dishes were resuspended in RPMI 1640 confining 10 percent FCS and incubated in nylon-wool columns (Feitwall Laboratories) for 45 minutes at 37°C and then underwent elution at 2 ml/min using warm medium. The final cell […]