Adventitious buds induction

By optimizing the culture conditions,the rate of adventitious bud induction,elongation and rooting reached to 100%,83%,and 100%,respectively.采用优化的培养基培养

Darkness or low intensity light at the beginning of adventitious bud induction was necessary with no difference for the incubating duration from 2 to 4 weeks.葡萄不定芽再生须经黑暗培养或弱光培养，暗培养时间在2周与4周之间再生效果差异不显著。

Callus and adventitious bud induction :The leave were cultured under the sunlight by using the improved liquid medium , MS+BA1+2, 4-D0.5. After two weeks, the bud differentiation rate was more than 1300%.诱导外植体的生长与分化：利用改进的液体培养基MS+BA1+2，4-D0.