Von Frey filaments can be used to determine tactile sensitivity using application of ascending force intensities. Any glabrous area can be probed, but most commontly the plantar surface of the hind paw is used. Each filament is designed to produce a given force (in g) precisely upon buckling. Thus, the minimum force to evoke a withdrawal response and the tactile threshold can be determined. Each filament is applied 10 times with 5-30 s random intervals. A reaction to a given stimulus was defined as rapid withdrawal of the hind paw when stimuli are applied and the number of responses to each stimulus intensity is determined. Tactile threshold is generally defined as a withdrawal response in 8 of the 10 trials to a given stimulus intensity. A higher number responses across stimulus intensities results in a lower tactile threshold and is interpreted and increased tactile sensitivity.