Dan Arlow

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Current Research

Nearly all efforts to engineer biology, including biomanufacturing of chemicals using fermentation, production of new vaccines or biological therapeutics, gene editing/gene therapy, and most biological experiments in general require the construction of 1-20 kilobase DNA molecules with a desired sequence. Today, most DNA constructs are created by amplifying, modifying, and combining existing template sequences using “molecular cloning” techniques that are time intensive and often fail for poorly understood reasons, resulting in lost time and missed research opportunities. We are developing new tools for rapid synthesis of multi-kilobase DNA that we hope will dramatically accelerate the “design-build-test-learn” cycle in bioengineering and ultimately bring about a new era of genome-scale synthetic biology.