NEBNext® Sample Preparation for Next Generation Sequencing

Preparation of high quality libraries at high yield is a critical first step in next generation sequencing workflows. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA and RNA and target enrichment of DNA for next generation sequencing. NEBNext kits are carefully designed to improve yields and library diversity using a broad range of input amounts from a wide variety of sample types. This has been most recently expanded with the addition of a novel enzyme-based DNA fragmentation system in the NEBNext Ultra II FS DNA Library Prep Kit (NEB #E7805), which enables reliable high-yield high-quality library construction with as little as 100 pg of input DNA.

The determination of the number of sequencing-ready molecules present after library preparation is an important step in the next generation sequencing (NGS)
workflow and has a strong influence on the success of both a sequencing run and a sequencing-based experiment.

NEB researchers published a paper in Science highlighting DNA damage as a prevalent source of errors in public cancer databases. Learn about how addressing this damage could improve the detection of low-frequency disease variants.

Target enrichment offers several advantages over more comprehensive genomic profiling for a variety of scientific and applications. During this webinar, we explore the advantages and limitations of target enrichment, and discuss how NEBNext Direct®, a novel solution for selective enrichment of genomic targets, addresses these challenges.