Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA), the principal inhibitory neurotransmitter in the brain. There are two isoforms of GAD, GAD65 (GAD2) and GAD67 (GAD1) (65 and 67 kDa, respectively) encoded by two different genes. The two isoforms have distinct intraneuronal distribution in the brain. There is 64% amino acid identity between the two isoforms. The highest degree of diversity appears to be located at the N-terminus, which in GAD65 is required for targeting the enzyme to GABA-containing secretory vesicles. GAD65 is primarily found in nerve terminals. GAD67 has been thought to be primarily located in the nerve cell body and has been identified as a major autoantigen in type 1 diabetes.

Reconstitute the lyophilized powder with deionized water (or equivalent) to antibody concentration of 0.5 mg/ml.

Specificity

GenScript Rabbit Anti-GAD65+GAD67 Polyclonal Antibody detects endogenous levels of mouse and rat GAD65 and GAD67 protein. This antibody reacts specifically with GAD65 and GAD67 isoforms. It is predicted to react with human GAD65 and GAD67 according to sequence homology.Positive Control: Mouse brain and rat brain

Species Reactivity

Mouse and rat. This product has not been tested for other species yet.

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.Western blot: 0.5-1 µg/mlImmunohistochemistry: 5-10 µg/mlOther Applications: user-optimized