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3/17 Dividend Street, Mansfield, 4122, Queensland, Australia phone: +61 7 3103 8560 email: info@kyratec.com web: www.kyratec.com Access all systems functions from here by pressing the icons with the stylus provided. Desktop 'Home' 1 Configuration takes the user to the configuration page allowing of machine options. PCR Run is used to load or create an new PCR thermal profile and initiate its execution. Manual Control allows immediate manual setting of block temperature. Information displays a general information screen such as serial number, software and hardware version numbers. An on-screen user manual is accessible from under this page menu

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3/17 Dividend Street, Mansfield, 4122, Queensland, Australia phone: +61 7 3103 8560 email: info@kyratec.com web: www.kyratec.com PCR Run Load an existing experiment from either the internal drive or an external USB drive, or create a new thermal profile using either the Wizard or the Manual Profile Builder. 2

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3/17 Dividend Street, Mansfield, 4122, Queensland, Australia phone: +61 7 3103 8560 email: info@kyratec.com web: www.kyratec.com Wizard Mode The Wizard utility enables the user to configure easy to moderate complex profiles in just moments. To adjust any of the parameters click on the corresponding time or temperature boxes. The Wizard screen displays each of the steps which occur in a typical range of profiles. At a minimum there are 3 steps, Preheat and then Denaturation and Anneal which are cycled. 3

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3/17 Dividend Street, Mansfield, 4122, Queensland, Australia phone: +61 7 3103 8560 email: info@kyratec.com web: www.kyratec.com Run Confirmation Displays the profile sequence in text form for user to review. Shows detail of what occurs on run completion. Select OK to proceed or Cancel further edit profile. System also allows profile to be run in virtual mode. 4

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3/17 Dividend Street, Mansfield, 4122, Queensland, Australia phone: +61 7 3103 8560 email: info@kyratec.com web: www.kyratec.com Creating New Events Hold – allows the operator to set the hold time and temperature. Ramp – allows the block temperature to be ramped either up or down between two set temperatures over a preset length of time. Pause – will hold the temperature at the last set point and pause the run with an audible warning. Cycling x Step – will proceed to generate a multi step cycling event. 6

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3/17 Dividend Street, Mansfield, 4122, Queensland, Australia phone: +61 7 3103 8560 email: info@kyratec.com web: www.kyratec.com When step parameters are entered the user is prompted for number of cycles if it is a cycling step. Numeric Input Touch Screen After this display returns to the profile screen with the new event displayed. 9

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3/17 Dividend Street, Mansfield, 4122, Queensland, Australia phone: +61 7 3103 8560 email: info@kyratec.com web: www.kyratec.com Run Screen Once the run commences the software will automatically proceed to the Run Screen. The thermal profile may not be edited once the run has commenced, however Pause, Start and Stop functions may be used. The Run Screen also displays status, elapsed and estimated run time, current user logged in and their profile picture. 10

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3/17 Dividend Street, Mansfield, 4122, Queensland, Australia phone: +61 7 3103 8560 email: info@kyratec.com web: www.kyratec.com Post Run Report On run completion the post run report screen will show and if enabled an audible alarm will sound. The alarm may be muted by pressing the mute alarm button. The post run report can be saved as a text file to a USB memory stick 11

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3/17 Dividend Street, Mansfield, 4122, Queensland, Australia phone: +61 7 3103 8560 email: info@kyratec.com web: www.kyratec.com File Manager: manages PCR run files and copying of file from the device to USB memory stick. Machine Settings: allows changes to be made to machine settings. Time & Date: used to set the Time for the instruments internal clock. Configuration The configuration page provides access to the following features. Thermal Calibration feature is used to adjust the machine calibration. User Accounts: for user account control. Software Updates: used to update SuperCycler operating Software. Touch Panel Calibration: allows calibration of the touch-screen interface using the calibration wizard. 12

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3/17 Dividend Street, Mansfield, 4122, Queensland, Australia phone: +61 7 3103 8560 email: info@kyratec.com web: www.kyratec.com The Oligo Calculator is useful for calculating the theoretical melt temperature and other information pertaining to the oligo design. Simply enter the sequence and the calculator will do the rest. Extras Several popular Games are also provided for your enjoyment! 16

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3/17 Dividend Street, Mansfield, 4122, Queensland, Australia phone: +61 7 3103 8560 email: info@kyratec.com web: www.kyratec.com Free software upgrades for the life time of the product are available for all customers via our website. Upgrade Software The upgrade process is very straight forward and takes only a few minutes. 17