RNAiMonitor—RNA Interference Assay

This RNA intereference assay is designed to monitor knockdown of RNAi target sequences efficiently and easily, using a no-cell-lysis protocol. It consists of the pRNAiMonitor-MetLuc2 Vector and the Ready-To-Glow Secreted Luciferase Reporter Assay. The pRNAiMonitor-MetLuc2 vector expresses MetLuc, a secreted luciferase reporter that can easily be detected in the medium surrounding the cells. When a target sequence (encoding the target of your shRNA or siRNA) is cloned downstream of the MetLuc gene, a bicistronic mRNA transcript encoding both the MetLuc and the target sequence is produced. Target gene knockdown results in the degradation (or translational inhibition) of the mRNA transcript, which causes a proportional decrease in the expression of the reporter protein. The reporter molecule is easily detected with the Ready-To-Glow Secreted Luciferase Reporter Assay kits.

Features

Applications

RNA interference assay, measures shRNA or siRNA knockdown

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.