Mentions:
The uptake of n-GO-PEG-MUC1 in MUC1+ cells was also assessedbyflow cytometry and the extent of uptake was expressed as mean fluorescenceintensity (MFI) (Figure 3). Cells were treated for 1, 3, and 24 h with anti-MUC1 IgG or n-GO-PEG-MUC1at concentrations equivalent to 2.5 μg/mL anti-MUC1 IgG. n-GO-PEGwas used as a control. At 1 h, comparable results were obtained forboth anti-MUC1 IgG and n-GO-PEG-MUC1 with uptake only being observedin MUC1+ cells. At 3 h, an increase in MFI was found in MUC1–cells treated with n-GO-PEG-MUC1. This increase in MFI, however, wassignificantly lower than that observed in MUC1+ cells. At 24 h, MUC1+cells treated with n-GO-PEG-MUC1 showed significantly higher MFI valuescompared to all treatment groups (Figure 3A), agreeing with CLSM and MP results.

Mentions:
The uptake of n-GO-PEG-MUC1 in MUC1+ cells was also assessedbyflow cytometry and the extent of uptake was expressed as mean fluorescenceintensity (MFI) (Figure 3). Cells were treated for 1, 3, and 24 h with anti-MUC1 IgG or n-GO-PEG-MUC1at concentrations equivalent to 2.5 μg/mL anti-MUC1 IgG. n-GO-PEGwas used as a control. At 1 h, comparable results were obtained forboth anti-MUC1 IgG and n-GO-PEG-MUC1 with uptake only being observedin MUC1+ cells. At 3 h, an increase in MFI was found in MUC1–cells treated with n-GO-PEG-MUC1. This increase in MFI, however, wassignificantly lower than that observed in MUC1+ cells. At 24 h, MUC1+cells treated with n-GO-PEG-MUC1 showed significantly higher MFI valuescompared to all treatment groups (Figure 3A), agreeing with CLSM and MP results.