How Does a p24 ELISA Work?

The virus in your supernatant is first lysed in the supplied buffer system to release p24. The sample is then applied to 96-well microtitration plate (made up of 12 separable 8-well strips), in which the wells are pre-coated with murine anti-p24 capture antibody. After washing to remove unbound lysate, the amount of specifically bound p24 is then detected using a biotinylated anti-p24 secondary antibody, streptavidin-HRP (horseradish peroxidase), and a color development reagent. The kit is supplied with a p24 control which is used to generate a standard curve and calibrate the p24 equivalent of your supernatant, and its titer.

Why You Need To Know Your Titer

Confirm the success of your packaging reaction to avoid wasting time with your experiments

Perform consistent experiments

Ensure sufficient MOI so that 100% of your cells are transduced

Control copy number and hence expression levels

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.