Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Applications

Specificity and Use

This synthetic peptide corresponded to a region near the C-terminus of this gene product, and was 100% conserved between the human (Q99259, NCBI), mouse (P48318, NCBI) and rat (NP_058703, NCBI) gene products.

Usage:

This GAD67 antibody recognizes only a subpopulation of GABA-ergic neurons in the cerebellar cortex, and the basis of this subpopulation is unknown. In stain cerebellar cortex, only a subpopulation of Purkinje and basket cells stain as is true for other inhibitory populations. 2% paraformaldehyde-fixed tissues recommended for immunohistochemistry and immunocytochemistry.

About GAD1 / GAD67

Description: GAD1 / GAD67 is one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome.