The aim of this research is to investigate any effects of mobile phone usage on human brainwaves using electroencephalograph (EEG) particularly on alpha wave. EEG signals were recorded from thirty samples that make calls from a mobile phone to another party without conversation. The mobile phone is strapped to the right ear. The EEG recording took place before, during and after the mobile phone calls. In addition, samples will be interviewed with questions related to the usage of hand phones prior to EEG recording. The brainwave signals were analyzed using statistical analysis. The EEG result shows that the alpha level of the right side decreases significantly during the calls and further decreases within the period of five minutes after the calls were ended. However, the alpha level of the left side remains consistent throughout the experiment. It follows that the correlation between the left and the right brainwaves signal decreases significantly during the calls and further decreases within the period of five minutes after calls. There is evidence that the usage of mobile phones affect the alpha brainwaves.