Target Details IL-13

IL-13, also known as NC30, is a 12.5 kDa protein secreted by activated T helper cells, CD8+ T cells, and NK cells. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression and suppression of inflammatory cytokine expression.

Application Details

Applications reported: PVM13-1 has been reported for use in intracellular flow cytometric analysis, cytokine neutralization, and ELISA. (Fluorochrome conjugated PVM13-1 is recommended for use in intracellular flow cytometry.) (Please use Functional Grade purified PVM13-1, cat. 16-7139, in functional assays.)

Applications tested: The PVM13-1 antibody has been tested as the capture antibody in a sandwich ELISA and ELISPOT assay for analysis of human interleukin-13 (IL-13) in combination with the biotinylated anti-human IL-13 polyclonal antibody (13-7138) antibody for detection and recombinant human IL-13 (14-8139) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.