Custom antibody development

We offer world-leading antibody development platforms based on a proprietary RabMAb® rabbit monoclonal platform, a phase display platform (AxioMx), and a next-generation sequencing platform (NGS-RabMAb®)

Asparaginase (EC 3.5.1.1) is a homotetramer that catalyzes the hydrolysis of asparagine to aspartic acid and ammonia and exhibits about a 2-4% activity on glutamine and 5% on D-asparagine. Asparaginase does not occur naturally in humans but is found in bacteria, plants and many animals (e.g. guinea pigs).Asparaginases can be used for different industrial and pharmaceutical purposes. The most common use is in food manufacturing as food processing aid to reduce acrylamide, a suspected carcinogen, produced in fried starchy food products. Metabolization of asparaginase prevents acrylamide formation in fried foods (Maillard reaction).Other asparaginases are used to treat acute lymphoblastic leukemia (ALL) and some other hematopoietic neoplasms (e.g. multiple myeloma). The enzyme’s antineoplastic effects are based on the inability of cancer cells (unlike healthy cells) to synthesize asparagine. However, the enzyme is not without some antigenicity and toxicity so it is very important to measure its activity in biological samples or monitor its activity during therapy.

It is ideal to collect serum from fresh blood samples, aliquot them and store at -80 degrees C for short periods of time (a couple of months) for this assay. EDTA is usually avoided in enzymatic assays because of its chelating properties. I a...

This assay works on the principle of the Asparaginase present in your samples to hydrolyze asparagine to produce aspartic acid. This aspartic acid has to be further converted to another intermediate which finally reacts with the probe on order to gener...

Thank you for your enquiry and interest in our products. This assay kit (ab107922) works on the principle of the Asparaginase present in your samples to hydrolyze Asparagine to produce Aspartic acid. This Aspartic acid has to be further converted ...

we are not so certain that whole blood can be used as a sample type with this kit.The red colour of the blood will interfere significantly with the final reading. We would recommend using plasma/serum samples for this purpose. Plasma/serum ca...