Gallery: Biotinylated Protein Ladder Detection Pack #7727

Product Description

Biotinylated protein ladder detection packs are designed to detect the molecular weight ladders on Western blots when using the horseradish peroxidase (HRP) based Western detection system. The pack contains biotinylated protein ladders and HRP-linked, anti-biotin antibody. The molecular weight ladders are a mixture of purified proteins covalently coupled to biotin that resolve to 10 bands that have a size range of 9-200 kDa. The anti-biotin antibody is used to detect biotinylated protein ladders on Western blots. The packs have been optimized for chemiluminescent Western detection procedures.

Detection of Protein Ladders

Perform chemiluminescent detection according to the manufacturer’s instructions except, at the stage of secondary antibody incubation with the blot, concurrently add the anti-biotin antibody (provided) at a dilution of 1:1000. Incubate for one hour at room temperature with gentle agitation.