Congratulations for your results . This forum is indeed very helpful. Whatever questions I have in my mind I just come here and ask....the good thing is we get quick reply if we ask the questions in a right way (everybody should be able to understand what we want to ask). Thanks to all members and of course to Bioforum to make this platform for us

The final concentration of the reagents:
0,2mM of each dNTP
0,5uM of each primer (I'm still trying with less primer)
1U/20uL of PfuTurbo Cx HotStart DNA Polymerase
60ng of DNA (quantified in Nanodrop - using factor '33' for ssDNA)

That is nice. thanks for sharing. Phage434 explained why lowering extension temperature is important in another post as shown below.

The EXTENSION temperature should be lower as well. Many high AT regions will not extend at 72. Try extending (for longer times (double)) at 63 or 65. You are using a Taq polymerase, correct? High fidelity polymerases will not read the uracils in bisulfite modified DNA. How are your primers designed? Are you certain they are correct (it is very easy to get confused!)