Isolation of Genomic DNA from Whole Blood—NucleoMag Blood

NucleoMag Blood 200 µl is designed for the rapid parallel purification of genomic DNA from whole blood in a 96-well format. After lysis of blood cells, genomic DNA is selectively bound to the NucleoMag B-Beads. Contaminants are washed away and the DNA can be eluted from the beads under low-salt conditions. Since the magnetic bead technology provides a closed system (no transfer of sample from one well to another is necessary during the preparation), the risk of cross-contamination is reduced. NucleoMag technology can be easily adapted to common liquid handling instruments: It can be used either with static-pin separators (e.g., NucleoMag SEP) or magnetic separators integrated into robotic workstations.

Typically, 96 samples can be purified in 45 minutes on common automation platforms. The kit provides reagents for the purification of 2–8 µg genomic DNA from 200 µl samples of whole blood. The purified DNA is ready to use for PCR, Southern blotting, and any enzymatic reactions.