Z-focal series of HeLa cell at metaphase stained for DNA (blue), phospho-Ser92-MAK (mitotic centromere-associated kinesin) (red), and Aurora B (green). Phosphorylation of MCAK is reduced in Bod1siRNA cells. Cells were transfected with control (this image) or Bod1 (CIL: 13378) siRNA. After 72 h, cells were treated with monastrol for 3 h and released into media containing MG132 for 1 h before fixing. Cells were stained for total mitotic centromere-associated kinesin (MCAK) population. MCAK localizes to the inner centromere in its phosphorylated form but concentrates at kinetochores in its dephosphorylated state.