Associated products

Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

ICC/IF

1/100 - 1/400.

WB

1/1000 - 1/8000. Predicted molecular weight: 192 kDa.

IHC-Fr

1/1000 - 1/4000.

IHC-P

Use at an assay dependent concentration.

IP

Use at an assay dependent concentration.

Target

RelevanceMembrane vesicle formation is a process required for the endocytosis and biosynthesis of various secreted and membrane bound proteins. Clathrin is a protein which assembles into a polyhedral network on the cell membrane as the membrane invaginates, forming a coated pit which is essential to endocytosis. Clathrin is composed of three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chains which combine to create a distinct three-legged triskelion. It is this morphology which allows Clathrin to form its unique polyhedral network.

Thank you for your call today and for letting us know about the trouble with this antibody. I sincerely apologize that this problem was not initially resolved, and if there is anything else that we can do for you please let me know.