In the episode {Trials and Tribble-ations} the names of the Federation temporal investigators "Dulmer" and "Lucsly" are anagrams of "Mulder" and "Scully" from The X Files.

In one episode, while Kira is still pregnant the O'Briens' baby, she screams at Dr. Bashir (Alexander Siddig) "This is all your fault!" This is an in-joke referring to the fact that actress Nana Visitor and Siddig were expecting a child in real-life.

According to an April 2003 interview, Avery Brooks was initially required by contract to appear with hair in order to prevent confusion with his [Spenser: For Hire] character, Hawk. In later seasons, as Sisko became established, Brooks was allowed to shave his head and re-grow his beard.

The Star Trek Crews from all the [Star Trek] series were ranked #2 in [TV Guide]'s list of the "25 Greatest Sci-Fi Legends" ({1 August 2004 issue}).

The tankards used for Klingon bloodwine are actually measuring cups used in baking.