Supplementary Material

Supplementary Material 1: Three tables showing normalized values (R) of the three groups of samples GSCs, dGSCs and ASCs with the ten studied HKGs. R were calculated using formula and depend on E the efficiency of each primer (1).

Supplementary Material 2: Two tables describing the ΔCt comprative method for dGSCs and ASCs and the ranking of the ten HKGs according to StdDev mean of each gene.

Supplementary Material 3: Mineral nodules were observed for dGSCs at different time points D7, D14 and D21. This confirmed the osteogenic differentiation in accordance with the increase of RUNX2 gene expression. Alizarin Red S staining at D21 confirmed the calcium content of these nodules.

Supplementary Material 4: MIQE checklist was almost respected by the different steps of our study, from collecting samples to gene expression analyses, which is in accordance with the accuracy of our results.