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Notes: The PowerPlex® 16 System was used to genetically type 354 people from four distinct tribes (Hmar, Lai, Lusei, and Mara) in Northeastern India. Allele frequencies and population statistics are provided. (3004)

Notes: The authors generated allele frequency data for 100 unrelated individuals from North Portugal and 110 unrelated individuals from Mozambique using the PowerPlex® 16 System, GenePrint® CTTv Multiplex and Profiler Plus™ kit. Four inconsistencies in genotypes between the kits were detected, three for D5S818 and 1 for D8S1179, due to primer-binding site mutations. (3862)

Notes: The authors used the PowerPlex® 16 System to type 526 Japanese individuals. Allele frequencies for 13 CODIS loci were determined with the PowerPlex® 16 System and AmpFlSTR® Identifiler® kits. (2823)

Notes: Allele frequencies for 11 autosomal STR loci were determined in the Yan Bian region of China. Population data for nine STR loci were determined using the AmpFlSTR® Profiler Plus™ kit and for the Penta D and Penta E loci using the PowerPlex® 16 System. DNA was isolated using phenol:chloroform extraction, and 1ng was amplified as directed by the manufacturer. Amplified products were detected using an ABI PRISM® 377 DNA Sequencer. (3826)

Notes: In this paper, researchers report allele frequencies for a population in Northeastern China. Data was generated using the PowerPlex® 16 System. DNA from volunteers was extracted using a phenol chloroform method. (3000)

Notes: The authors generated population data for 723 individuals living in four regions of Hungary. DNA was collected as blood samples and amplified using the PowerPlex® 16 System. Amplified products were detected using the ABI PRISM® 377 DNA Sequencer or 310 Genetic Analyzer. (3866)

Notes: The authors reported population data for the Penta E and Penta D loci in 160 Portuguese Caucasians, 19 Portuguese of African origin and 150 meioses. DNA was extracted from blood samples using Chelex® resin, then purified using the Wizard® DNA Clean-Up System. Amplifications were performed using the PowerPlex® 16 System, the SGM Plus® kit and the GeneAmp® PCR System 9600. Amplified products were detected using an ABI PRISM® 377 DNA Sequencer. (3845)

Notes: The authors generated population data from 508 unrelated Japanese individuals using the PowerPlex® 16 System. DNA was collected as buccal swabs and isolated using Chelex® resin followed by phenol:chloroform extraction and ethanol precipitation. For each PowerPlex® 16 reaction, 2ng of DNA was amplified using a GeneAmp® PCR System 9700, and amplified products were detected using the ABI PRISM® 377 DNA Sequencer. (3843)

Notes: In this paper, researchers from both Promega and state crime laboratories provide an evaluation of the Powerplex® 16 System loci (CODIS, Penta D, Penta E, and amelogenin). Data from several laboratories using a variety of thermal cyclers and the ABI PRISM® 310 Genetic Analyzer or ABI PRISM® 377 DNA Sequencer are presented. The Powerplex® 16 System was able to consistently genotype samples from as little as 0.0625-2ng of genomic DNA template. Other factors such as reaction volume, annealing temperature, titration of AmpliTaq Gold® DNA Polymerase, magnesium, or primer pairs, and 1ng DNA mixtures were analyzed using the Powerplex® 16 System. The researchers also present data on stutter analysis and cross reactivity of primer sets on primate DNA templates for each loci. (2675)

Notes: The authors identified two microvariant alleles (18.4 and 19.4) at Penta E in the Japanese population. Both microvariant alleles had a partial repeat motif of AAAA rather than the AAAGA repeat. The authors discuss their ability to resolve alleles differing in size by only one base (i.e., 18.4 and 19) by capillary electrophoresis. (3970)

Forensic Sci. Int.126, 178–86.
Allele frequencies for STR loci of the Powerplex 16 multiplex system in five endogamous populations of India.2002

Kashyap, V.K., Sarkar, N. and Trivedi. R.

Notes: Population data for five populations in India were determined using the PowerPlex® 16 System. Blood was collected from 95 unrelated individuals, and DNA was isolated by phenol:chloroform extraction. Two nanograms was amplified, and amplified products were detected using an ABI PRISM® 377 DNA Sequencer. (3831)

Forensic Sci. Int.126, 173–7.
Distribution of alleles of 15 STR loci of the Powerplex 16 Multiplex system in four predominant population groups of South India.2002

Rajkumar, R. and Kashyap, V.K.

Notes: The authors examined allele frequencies for 15 autosomal STR loci in four population groups in southern India using the PowerPlex® 16 System. DNA was isolated from blood using phenol:chloroform extraction, 2ng was amplified and amplified products were detected using an ABI PRISM® 377 DNA Sequencer. (3833)

Forensic Sci. Int.128, 196–201.
Genetic diversity at two pentanucleotide STR and thirteen tetranucleotide STR loci by multiplex PCR in four predominant population groups of central India.2002

Sarkar, N. and Kashyap, V.K.

Notes: Population data was determined for 208 unrelated individuals from central India using the PowerPlex® 16 System. DNA was isolated from blood by organic extraction, and 2ng was amplified per reaction. Amplified products were detected using an ABI PRISM® 377 DNA Sequencer. (3829)

Notes: The authors generated STR allele frequency data from three minor caste groups in Bihar, India. DNA was isolated from using phenol:chloroform extraction, and STR loci were amplified using the PowerPlex® 16 System. (3974)

Forensic Sci. Int.129, 78–80.
Genetic profile of a multi-ethnic population from Guine´-Bissau (west African coast) using the new PowerPlex 16 System kit2002

Gonçalves, R., Jesus, J., Fernandes, A.T. and Brehm, A.

Notes: The PowerPlex™ 16 System was used to type 100 males from Guine´-Bissau in West Africa. DNA was isolated from each individual using a chelex method. Allele frequencies for the population are provided. (3010)

Notes: The authors generated population data for three tribal populations from Orissa, India, using the PowerPlex® 16 System. DNA was isolated from blood samples using phenol:chloroform extraction, and 2ng was amplified per reaction. Amplified products were detected using an ABI PRISM® 377 DNA Sequencer . (3852)

Forensic Sci. Int.128, 190–5.
Genomic diversity at 15 fluorescent labeled short tandem repeat loci in few important populations of State of Uttar Pradesh, India.2002

Tandon, M., Trivedi, R. and Kashyap, V.K.

Notes: Population data were determined for 191 unrelated individuals in India using the PowerPlex® 16 System. DNA was isolated from blood using phenol:chloroform extraction, 2ng of DNA was amplified and amplification products were detected using an ABI PRISM® 377 DNA Sequencer. (3832)

Notes: The PowerPlex® 16 System was used to calculate allele frequencies at 15 autosomal loci in 356 unrelated individuals from four populations in India. DNA was extracted from blood samples by phenol:chloroform extraction, and 2ng was amplified; amplified products were detected using an ABI PRISM® 377 DNA Sequencer. (3828)

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