DIAKEY Free T4 ELISA

Intended Use

Enzyme immunoassay for quantitative determination of Free T4 in human serum or plasma

Principle of the Assay

The assay is a solid-phase, competitive immunoassay (ELISA). During the incubation, the sample/calibrator FT4 antigen competes with the T4 antigen in streptavidin coated microplates with HRPO labeled T4 antibody. After washing, Chromogen reagent is added to each well and the enzyme reaction is allowed proceed. During the enzyme reaction a blue color will develop if antigen is present. The intensity of the color is proportional to the amount of FT4 present in the samples. The color intensity is determined in a microplate spectrophotometer at 450nm (reference 620nm).

Handling Precaution

Do not use mixed reagents from different lots.

Do not use reagents beyond the expiration date.

Use distilled water stored in clean container.

Use an individual disposable tip for each sample and reagent, to prevent the possible cross-contamination among the samples.