The RNeasy 96 Kit provides high-throughput purification of total RNA from up to 96 cultured-cell samples using silica-membrane RNeasy 96 plates. Manual purification is carried out using the QIAGEN 96-Well-Plate Centrifugation System and/or the QIAvac 96 vacuum manifold. Automated purification using the related RNeasy 96 BioRobot 8000 Kit is performed on the BioRobot Universal System, which processes up to 96 or 192 samples per run followed by real-time RT-PCR setup.
For optimal results it is recommended to use this product together with QIAvac 96.

The RNeasy 96 system provides a standardized, fast, reliable method for isolation of high-quality RNA from large numbers of samples (see table). The simple procedure is the key to the speed and efficiency of RNeasy 96 technology. Cells can be grown and directly lysed in 96-well cell-culture dishes. After transfer of the lysates to the wells of the RNeasy 96 plate (see figure "RNeasy 96-well plate"), RNA binds to the silica membrane in each well, and contaminants are washed away. Pure RNA is then eluted in RNase-free water into individual collection tubes suitable for long-term storage and is ready to use for any experiment. Since the RNeasy procedure enriches for RNA species >200 nt, RNA yield does not include 5S rRNA, tRNAs, or other low-molecular-weight RNAs.

The key to the speed and efficiency of RNeasy 96 technology is the simple procedure. Cells can be grown and directly lysed in 96-well cell-culture dishes. After transfer of the lysates to the wells of the RNeasy 96 plate (see figure "RNeasy 96-well plate"), RNA binds to the silica membrane in each well, and contaminants are washed away. Pure RNA is then eluted in RNase-free water into individual collection tubes suitable for long-term storage and is ready to use for any experiment. The RNeasy 96 Kit includes protocols for total RNA isolation, cytoplasmic RNA isolation, and RNA cleanup. RNeasy 96 plates can be processed on the QIAvac 96 vacuum manifold and/or the 96-Well-Plate Centrifuge System.

RNA isolation can also be automated on the BioRobot 9604 workstation using the RNeasy 96 BioRobot 9604 Kit. Fully automated RNA isolation is possible on the BioRobot 8000 workstation or BioRobot Universal System using the RNeasy 96 BioRobot 8000 Kit.

One milliliter of milk contains approximately 50,000-200,000 leukocytes (with an average of approximately 100,000 leukocytes). In a BVDV-infected animal up to 1-30% of the leukocytes may be infected with the virus. Each infected leukocyte typically contains 10-10,000 BVDV entities.