We are looking at some cytoskeletal proteins which have varying
phosphorylation states. We would like to dephosphorylate them, and try
to look at them on immunoblots. We have phosphorylation independent and
dependent antibodies which we wish to use. I would imagine you would do
the dephosphorylation prior to running the Western, but I'm not sure if
you could dephos on the blot as well. In any case, has anyone had
success doing this before?
gerri