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In May, the end of the premiere of Glee — a Fox comedy series about a high-school choir in small-town Ohio — found the ragtag group of misfits belting out a brassy rendition of Don’t Stop Believin’.

Soon after, the 1981 song shot to No. 1 on iTunes.

Not the original Journey version — but the one by the young Glee singers.

The tune was also featured in a Family Guy episode from 2005 on Fox: The animated characters became one-night celebrities after singing the song karaoke-style (and a widow nearby was instantly snapped out of her grief by the wafting strains).

Don’t Stop Believin’ was used last year in a Hillary Clinton campaign video and in recent years on the TV shows Scrubs, South Park and Laguna Beach.

And, in 2007, the song saw perhaps its widest — and oddest — pop-culture exposure: as the closing music for the finale of the HBO mob drama The Sopranos, whose last scene abruptly cut to black.

Such modern ubiquity baffles Journey members, who composed the hit in less than three hours.

“It’s sort of a cosmic thing,” said keyboardist Jonathan Cain, who wrote the song’s opening piano riffs — among the most recognizable in modern pop music.

“This song is still speaking to a generation, even 25 years later. That’s something every songwriter and singer could ever hope for.”