Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. This recombinant human MMP-15 catalytic domain has a purity of >90% on SDS-PAGE. It can be used directly in FRET-based enzyme activity assay or served as positive control for zymography, Western blot, and ELISA.