Wheat take-all, caused by Gaemannomyces graminis var. tritici,has been observed in recent years in different areas of Iran. In this study, in order to investigate the possibility of biological control of pathogen, seven isolates Trichoderma include T. harzianum S, T. harzianum A, T. harzianum M, T. koningi, T. longibrachiatum and T.virens and four isolates of Talaromyces include Talaromyces flavus 134, Talaromyces flavus 136, Talaromyces flavus 75 and Talaromyces flavus 60 used against the pathogen by extracellular fluid method in vitro condition. The experiment was arranged as randomized complete design with 3 replication. The effect of extracellular fluid of antagonistic isolates on mycelia growth of Ggt indicated that the extracellular fluid of Talaromyces flavus 136، T. harzianumand T. harzianum S isolates the rate of 5 ml after 72 hours of rate (41/51, 40/88 and 37/73 %) and T. longibrachiatum isolate at rate of 10 ml at two times of measurement (52/83 and 52/38%) had the most inhibition effect on the growth of pathogen. This study has been the first study related to biological control of wheat take-all by T. flavus.