tetrqacylin and GFP in Pseudomonas sp. (fwd)

derrampa at uoguelph.ca (Deena Errampalli) wrote:
>>Dear fluoronetters,
>>In our lab, we have transformed Pseudomonas sp. with green fluorescent
>protein (gfp) and the tetracyclin was the antibiotic marker. Southern
>hybridizations indicated that the transformants do contain gfp. When I
>checked the transformants for the stability of the gfp insert,
>I have noticed that the transformants fluoresced only on the medium with
>tetracycline but not on the medium without tetracycline. I transferred
>the non-fluorescing colonies from the medium without tetracyclin to fresh
>medium with and without tetracyline. Again, I found that the colonies on
>tetracyclin fluoresced while the clonies on the medium without
>tetracyclin did not fluoresce.
>>Here are my questions,
>1. Did any one of you notice similar tetracyclin related problems?
>2. Does the tetracyclin itself cause the bacteria to fluoresce?
Could your GFP be inserted near a tetracyclin activated promoter ?
Jacques