Background and Aim: Baneh (Pistacia Atlantica) is a valuable oily fruit that has gained considerable attention because of its pharmaceutical, nutritional and industrial applications. This study aimed to investigate the antinociceptive effect of rubbing baneh oil using formalin test during acute and chronic phases in male laboratory mice.Materials and Methods: This experimental study was conducted on 30 male mice. They were allocated into 5 groups including control (without treatment), rubbing vaseline, morphine (positive control), baneh oil, and baneh oil plus morphine. Before the formalin test, the animals were treated with rubbing baneh oil for six hours. Morphine (10 mg/kg) was injected with a single dose subcutaneously. Pain scores were obtained using formalin test by injecting 0.02 ml of 2.5% formalin into the animals’ right palm.Results: Rubbing baneh oil signiﬁcantly reduced the pain in acute pain (P <0.01), sub-acute pain (P <0.001), and chronic pain (P <0.001) phases as compared with the control group. On the other hand, baneh oil showed a stronger antinociceptive effect in chronic pain than morphine (P <0.01).Conclusion: This study showed that rubbing Baneh oil has antinociceptive effect where a considerable share of this effect may be attributed to the oil’s compounds.