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Backbone

Growth in Bacteria

Bacterial Resistance(s)

Ampicillin

Growth Temperature

30°C

Growth Strain(s)

NEB Stable

Growth instructions

Can use Stbl3 at 30°C or DH5alpha at 37°C. Carbenicillin is preferred over ampicillin. In DH5alpha this plasmid may act more like a high copy plasmid, although in Stbl3 it may act more like a low copy plasmid.

Resource Information

Depositor Comments

The plasmid is fully sequenced in the coding sequence regions (fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.

These plasmids were created by your colleagues. Please acknowledge the
Principal Investigator, cite the article in which the plasmids were described,
and include Addgene in the Materials and Methods of your future publications.