Abstract

Mid-infrared (mid-IR) spectroscopy provides a unique chemical fingerprint of biomaterials, including DNA and proteins, from single molecules to highly organised structures and, ultimately, to live cells and tissues. However, acquiring good signal–to–noise mid-IR spectroscopic images, at the cellular level, typically involves a synchrotron, with imaging times of order of minutes. Here we use a new laser-based table-top IR spectroscopic micro-imaging system, to obtain vibrational fingerprint signatures of living human ovarian cancer cells at a diffraction limited spatial resolution, and at a spectral resolution (< 20 cm−1) sufficient to map out the spatial distributions of chemical moieties inside the cell itself. The bright laser pulses give very high signal–to–noise images, and ∼100 psec image acquisition times that are roughly 1011 times faster than current mid-IRspectroscopic imaging techniques. The imaging method is quantitative, non-phototoxic, marker-free and easily fast enough to “freeze” moving, living specimens. It can be applied to a range of cell-level biochemical processes, and we believe it could impact on the fields of drug action, cell physiology, pathology and disease as a whole.

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