The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-CREB (Ser133) in a multiplexed assay.

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For research use only. Not for use in diagnostic procedures. Please note this kit does not contain all buffers necessary to run the assay, and must be used with an AlphaLISA SureFire Ultra assay kit to obtain all required reagents.

Alpha SureFire assays are cellular assays used to detect and quantitate phosphoproteins for cell signaling, pathway analysis, and cellular kinase studies using homogeneous (no wash steps; no separation steps) Alpha technology. Terbium SureFire assays enable multiplexing for detection of two phosphoproteins in a single well, when used in conjunction with any AlphaLISA SureFire Ultra kit. This multiplex measurement is achieved by the use of two types of Alpha Acceptor beads that emit at distinct wavelengths (Terbium: 545 nm, Europium: 615 nm). The two distinct Alpha Acceptor beads report their binding to distinct antigens through their association with specific assay antibodies.

The Terbium “Alpha 545 Acceptor Beads” in the kit are conjugated with a “CaptSure2” agent, which binds a specific CaptSure2 tag on one of the assay antibodies. In combination with the biotinylated antibody provided, the 545 nm signal generated will report levels of the phosphoprotein of interest (Target 2). This kit is to be used in conjunction with the reagents in an AlphaLISA SureFire Ultra kit, which will report that target-of-interest by Europium emission at 615 nm (Target 1).

Formats:

Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 60 µL reaction volume (30 µL of lysate).

Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume (10 µL of lysate).

Our 10,000 assay point kit allows you to run 10,000 wells in in 384-well format, using a 20 µL reaction volume (10 µL of lysate).

Our 50,000 assay point kit allows you to run 50,000 wells in in 384-well format, using a 20 µL reaction volume (10 µL of lysate).