The kit correctly scored 7 of the 8 samples. Due to the early phase of vaccination, sample 4 tested negative for IBDV-specific antibodies.

Principle

Infectious bursal disease is caused by infectious bursal disease virus (IBDV). The virus has an affinity to the bursa of Fabricius in chickens and it gives rise to an acquired B lymphocyte deficiency. The disease has mortality rates of 50 to 90%.

flocktype IBDV Ab is an ELISA (enzyme-linked immunosorbent assay) with ready-to-use colored reagents. The test plate is coated with a recombinant structural protein from the virus. This protein is highly conserved amongst infectious bursal disease virus strains and it is considered to be the major immunogenic peptide.

During the sample incubation, IBDV-specific antibodies bind to the immobilized antigen. The anti-IgY-HRP conjugate detects antibodies bound to the antigen. The color reaction is started by adding the substrate solution and stopped after 10 minutes. The optical density (OD) is measured in a spectrophotometer. The OD values correlate with the concentration of infectious bursal disease virus antibodies in the sample.

Procedure

Serum and plasma samples can be tested after dilution.

Samples are incubated for 30 min with the test plate. If antibodies to infectious bursal disease virus are present, they will bind to the antigen on the plates. Unbound material is removed by rinsing.

The anti-IgY-HRP conjugate is added. Unbound conjugate is rinsed out.

The colour reaction is started by adding the TMB Substrate Solution and stopped after 10 minutes. The optical density (OD) is measured using a spectrophotometer. The results are easy to interpret.

Applications

flocktype IBDV Ab is for the detection of antibodies to infectious bursal disease virus in serum and plasma samples from chickens.