Confocal RESCue

RESCue mode is Abberior Instruments’ unique & patent protected method for reducing the amount of photons sent into the sample while keeping image quality and resolution uncompromised.

Great news: it is not limited to STED imaging! For confocal microscopy, too, RESCue significantly reduces the light dose by up to 90% and more – again without losing any of the advantages of confocal imaging: speed, confocal resolution, z-sectioning ability, etc., are unchanged, while signal-to-noise, photobleaching behaviour, and live-cell survivability are significantly enhanced.

Check how we do live-cell confocal time-lapse imaging in confocal RESCue mode in an overnight measurement campaign with 14 hours of total imaging time. The cumulative light dose reduction was between 60% and 81% depending on the structure imaged.

Vero cells immunostained for the mitochondrial protein (PDH) and a peroxisomal membrane protein (PMP) using Cy3 and Cy5 conjugates. Z-stacks were recorded using standard confocal microscopy resulting in strong photobleaching after five consecutive stacks. Using confocal RESCue reduced photobleaching markedly and maintained image brightness after five consecutive Z-stacks.

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Live fibroblasts were stained for tubulin using SiR-tubulin (silicone-containing rhodamine, Spirochrome) and mitochondria (MitoTracker Red CM-H2XRos, ThermoFisher) and imaged in HDMEM-GFP2 (Evrogen).

During 12 hours of live cell imaging, confocal RESCue allowed for a 82% reduction of laser irradiation for MitoTracker and a 50% reduction for SiR-tubulin.

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Live fibroblasts were stained for tubulin using SiR-tubulin (silicone-containing rhodamine, Spirochrome) and mitochondria (MitoTracker Red CM-H2XRos, ThermoFisher) and imaged in in HDMEM (ThermoFisher).

During 14 hours of live cell imaging, confocal RESCue allowed for a 63% reduction of laser irradiation for MitoTracker, and a 53% reduction for SiR-tubulin.