This Request for Comments (RFC) suggests a framework for the description,
exchange and interlinking of Synthetic Biology data. The framework would
create an open process for the evolution and “rolling” standardization ...

The physical assembly of standard parts is currently a non-standard process, which can either
come from direct genome PCR with restriction enzyme sites incorporated into the PCR primers,
or overlap assembly PCR. ...

The BioBytes assembly method provides a new high-throughput way of assembling
plasmids as well as other large DNA constructs. Using a bead-linked assembly of genetic
parts with long (12 bp) standardized sticky ends, ...

“Part Flavors” are a means of describing the grammatical properties of parts. Specifically, this is introduced as a means of describing parts that encode peptides (CDS parts), but there may be value to extrapolating the ...

This RFC94 describes an assembly standard based on the Type IIS restriction enzymes BsaI and BbsI (also called BpiI). This assembly standard is based upon the Modular Cloning (MoClo) assembly strategy, which was introduced ...

Current, there is well adopted documentation of biobricks. The Registry of Standard Biology Part set the standard documentation for biobrick. However, there is no standard way to document a genetic circuit, what information ...

We present an updated, clean draft for additional public comment of a new legal framework supporting the future of biotechnology. The framework, named here as the BioBrick Public Agreement, is based upon the contribution ...

We present a draft for public comment of a new legal framework supporting the future of biotechnology. The framework, named here as the BioBrick Public Agreement, is based upon the contribution of promises not to assert ...

Construction of complex biological systems can require assembling many
modules together. However, existing assembly schemes are lacking in
generality, ease of use, or power to perform some desirable
operations. Currently, ...

The next generations of both biological engineering and computer engineering demand that control be exerted at the molecular level. Creating, characterizing and controlling synthetic biological systems may provide us ...

Protein fusions are currently not supported by most assembly standards. This assembly standard aims to provide the basis of a solution to a biobrick-friendly protein fusion mechanism that supports the current favorite ...

The purpose of this RFC is to describe a method that allows the design of protein domain based parts, starting with gene centered information and translate these informations into BBF RFC 25 compatible part. The method is ...

In this thesis, I present an engineering discipline for obtaining complex, predictable, and reliable cell behaviors by embedding biochemical logic circuits and programmed intercellular communications into cells. To accomplish ...

We propose a biochemically plausible mechanism for constructing digital logic signals and gates of significant complexity within living cells. These mechanisms rely largely on co-opting existing biochemical machinery and ...