RT Journal Article
SR Electronic
T1 Phagocytosis: flow cytometric quantitation with fluorescent microspheres
JF Science
JO Science
FD American Association for the Advancement of Science
SP 64
OP 66
DO 10.1126/science.7053559
VO 215
IS 4528
A1 Steinkamp, JA
A1 Wilson, JS
A1 Saunders, GC
A1 Stewart, CC
YR 1982
UL http://science.sciencemag.org/content/215/4528/64.abstract
AB The phagocytosis of uniform fluorescent latex particles by pulmonary macrophages in the rat was analyzed by flow cytometric methods. The percentage of phagocytic macrophages and the number of particles per cell were determined from cell-size and fluorescence histograms. A comparison of in vivo and in vitro phagocytosis data showed that the percentage of phagocytic lavaged macrophages reflected the availability of instilled particles. With sodium azide used to model phagocytosis inhibition, it was shown that the percentage of phagocytic cells and the number of particles per cell can be determined simultaneously.