Figure 5: BCS cells have a significantly higher rate of caveolin-independent
endocytosis than the differentiated breast cancer cells. A. Staining of the differentiated
breast cancer MCF-7 cells with LacCer in the absence of CVDE
inhibitor: top left, cells were stained with DAPI; bottom left, with BODIPYlabeled
LacCer; top right, amplified image of the cells from bottom left section;
bottom right, merge of the top left section and bottom left section. B. Staining
of the differentiated breast cancer MCF-7 cells with LacCer in the presence
of CVDE inhibitor MCD. C. Staining of the differentiated breast cancer MCF-7
cells with LacCer in the presence of CVDE inhibitor genistein. D. Staining of
the mammosphere cells derived from MCF-7 with BCS/A35 in the absence of
CVDE inhibitor: top left, cells were stained with DAPI; top right, with TR-labeled
BCS/A35; bottom left, merge of the top left section and top right section;
bottom right, amplified image of the cells from bottom left section. E. Staining
of the cells derived from mammospheres with BCS/A35 in the presence of
CVDE inhibitor MCD. F. Staining of the cells derived from mammospheres
with BCS/A35 in the presence of CVDE inhibitor genistein. G. Co-staining of
the cells derived from mammospheres with BCS/A35 and LacCer in the absence
of CVDE inhibitor: top left, cells were stained with DAPI; top right, with
BODIPY-labeled LacCer; bottom left, with TR-labeled BCS/A35; bottom right,
merge of the previous three sections. H. Co-staining of the cells derived from
mammospheres with BCS/A35 and LacCer in the presence of CVDE inhibitor
MCD. I. Co-staining of the cells derived from mammospheres with BCS/A35
and LacCer in the presence of CVDE inhibitor genistei