using citrate instead of fructose as reducing agent and increase concentration from 02/10/16 trial

comparing citrate to no citrate samples, and vary citrate concentration

Prepare 20ml reaction to synthesize large quantities of reactions known to work

SPI AuNP, WPI AuNP, and Casein AgNP as shown below.

Note that spectra of reaction mixes before reacting need to be taken

Also note that this is just one size of nanoparticle that can be made with these reactions, its possible to change variables and change size and functionality of nanoparticle, these were just the first reactions to work.

Detailed Reaction Setup shown Below (4 hours 80˚C, 1ml rxns)

20ml Reactions

'

'

'

'

'

'

'

'

Vial #

2.5mM HAuCl4 (µL)

5mMAgNO3 (µL)

Protein (µL)

10 mM Citrate (µL)

Water (µL)

Au/Ag:Protein

Notes

Casein 0.5

1

0

800

11200

0

8000

dark yellow nanoparticles

SPI 0.25

2

800

0

4000

400

14800

gold nanoparticles made

WPI 0.25

3

800

0

4000

400

14800

gold nanoparticles made

Pea Protein 0.25

4

0

800

4000

0

15200

protein agregates at bottom

2.5mMAgNO3

2.5mM HAuCl4

Citrate-Silver

Citrate-Gold

Casein

✓

x

––––––––––

x

Soy

x

x

✓

Whey

x

Pea

✓

x

✓

Rice

x

1mL Reactions

Vial #

2.5mM HAuCl4 (µL)

5 mMAgNO3 (µL)

Protein (µL)

10 mM Citrate (µL)

Water (µL)

Au/Ag:Protein

Notes

0.25 mg/mL Casein

1

40

0

560

40

360

-added 4 drops of 1M NaOH to dissolve, no reaction

Dilute from .5mg/ml Casein

2

40

0

560

60

340

no reaction

grams weighed: 12.91mg

3

40

0

200

40

720

no reaction

Stock: 26mL

4

40

0

200

60

700

no reaction

5

40

0

200

20

740

gold NPs w/ precipitate

6

40

0

200

40

720

gold NPs w/ precipitate

0.25 mg/mL Soy

7

40

0

100

0

860

gold NPs, fibers/aggregates?on sides w/ precipitate

grams weighed: 3.8mg

8

40

0

100

20

840

gold NPs w/ less precipitate

Stock: 15mL

9

0

40

560

40

360

slight NP (possibly due to citrate?)

10

0

40

560

60

340

slight NP (possibly due to citrate?)

11

0

40

200

40

720

slight NP (possibly due to citrate?)

12

0

40

200

60

700

slight NP (possibly due to citrate?)

13

40

0

200

20

740

gold NPs with some gold precipitate

14

40

0

200

40

720

gold NPs with some gold precipitate

0.25 mg/mL Whey

15

40

0

100

0

860

gold NPs with some gold precipitate

grams weighed:4.1mg

16

40

0

100

20

840

gold NPs with some gold precipitate

Stock: 16.5mL

17

0

40

560

40

360

slight NP (possibly due to citrate?)

18

0

40

560

60

340

slight NP (possibly due to citrate?)

19

0

40

200

40

720

slight NP (possibly due to citrate?)

20

0

40

200

60

700

slight NP (possibly due to citrate?)

0.15 mg/mL Pea

21

40

0

560

40

360

Added 20 Drops of 4M HCl, no rxn

Diluted from .25mg/ml

22

40

0

560

60

340

no rxn

grams weighed:7.8mg

23

40

0

200

40

720

no rxn

Stock: 31 mL

24

40

0

200

60

700

no rxn

25

40

0

560

0

400

add 10 drops 1M NaOH, no rxn

0.15 mg/mL Rice

26

40

0

560

20

380

no rxn

grams weighed: 1.5mg

27

40

0

200

0

760

no rxn

Stock: 10 mL

28

40

0

200

20

740

no rxn

29

0

40

560

0

400

AgNPs formed

30

0

40

560

20

380

AgNPs formed

31

0

40

200

0

760

AgNPs formed

32

0

40

200

20

740

AgNPs formed

Results

20ml Samples

SPI and WPI AuNP formed purple colored NPs

Casein AgNP formed gold colored NPs

Pea Protein solution remained clear with some white precipitation, possibly not total dilution