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These FcγR form hetero-oligomeric complexes with the same FcR γ-c

These FcγR form hetero-oligomeric complexes with the same FcR γ-chain, which contains an ITAM sequence required for cell activation and cell surface expression. Most FcγR-triggered responses are balanced by the signaling of inhibitory ITIM-bearing FcγRII. Via the simultaneous triggering of activating

and inhibitory signaling pathways, FcγR control a wide array of MLN2238 manufacturer cellular responses, including phagocytosis, antibody-dependent cell-mediated cytotoxicity and the release of inflammatory mediators, which ultimately lead to the amplification of normal and pathological immune reactions in vivo8–11. FcγR are expressed on many inflammatory cell types involved in allergic airway inflammation. It is, therefore, likely that FcγR, as well as polymorphisms in genes encoding FcγR, play a pivotal role in allergic airway disease 12. Allergen-specific IgG is present in the serum of allergic individuals and sensitized mice 13, and a specific role has been postulated for FcγRIII signaling in the regulation of optimal Th2-cell differentiation in allergy. This augmented Th2 differentiation was found to be independent of FcR-mediated antigen uptake and processing 14. Others 13 suggested that expression of FcγRI is important

during the sensitization phase of the development of allergic airway inflammation and airway hyperresponsiveness. buy Fer-1 In our study, we used a mouse model of experimental asthma to verify the impact of FcγR on antigen uptake and presentation by DC. We hypothesized that activating FcγR control the strength and characteristics of airway hyperresponsiveness and inflammation, and Meloxicam sought to demonstrate that IC can potentiate acute airway inflammation after sensitization, mediated by augmented T-cell proliferation after challenge. To compare the inflammatory response to inhaled antigens

in B6 and FcγR-deficient mice, the animals were sensitized with OVA+alum as described and challenged with OVA by inhalation. On day 3 after challenge, B6 mice revealed the characteristic perivascular and peribronchiolar infiltrate of mononuclear cells, whereas the allergic airway inflammation was reduced in FcγR-deficient mice (Fig. 1A). We observed significant eosinophilia in the BALF of B6 mice, which was virtually absent in FcγR-deficient (Fig. 1B). Control experiments of sensitized but non-challenged mice confirmed absence of eosinophilia and neutrophils in the BALF of all mice (data not shown). Both B6 as well as FcγR-deficient mice mounted a strong OVA-specific IgE response after sensitization, which resulted in equivalent mean OVA-specific IgE levels in both groups. No OVA-specific IgE responses were detectable in non-sensitized mice (data not shown). In order to confirm the constitutive FcγR expression on murine splenic and lung DC, cells from B6 mice were enriched by density gradient centrifugation and cell sorting of CD11c+MHC class II+ cells (Fig. 2A).