The MagAttract DNA Blood Mini M48 Kit provides automated purification of genomic DNA on the BioRobot M48 using proven MagAttract magnetic particle technology. DNA purification and magnetic separation take place in the pipet tips, increasing the efficiency of the procedure. Up to 48 samples can be processed per run in increments of 6 samples. Variable elution volumes enable easy optimization of DNA concentration.

The MagAttract DNA Blood Mini M48 Kit is designed for fully automated DNA purification. Proven MagAttract magnetic particle technology in combination with the BioRobot M48 workstation provides high-quality DNA, suitable for direct use in sensitive downstream applications. Advantages of the system include scalable sample volumes and elution volumes (see table ), giving the yield and concentration of high-quality DNA appropriate for the intended downstream application.

Sample and elution volumes for the MagAttract DNA Blood M48 procedure

Sample

Kit

Protocol

Amount of starting material (µl)

Elution volume (µl)

Blood

MagAttract DNA Blood Mini M48 Kit

200 µl Blood

100–200

50–400

Blood

MagAttract DNA Blood Midi M48 Kit

350 µl Blood

250–350

100–400

Blood

MagAttract DNA Blood Midi M48 Kit

700 μl Blood

500–700

200–400

Buffy coat enriched >9x

MagAttract DNA Blood Midi M48 Kit

75 μl Buffy Coat

50–75

150–400

Buffy coat enriched ≤9x

MagAttract DNA Blood Midi M48 Kit

150 μl Buffy Coat

100–150

150–400

Buffy coat with low leukocyteconcentration

MagAttract DNA Blood Midi M48 Kit

300 μl Buffy Coat

200–300

150–400

Procedure

MagAttract technology combines the speed and efficiency of silica-based DNA purification with the convenient handling of magnetic particles. Optimized buffers lyse samples, and DNA binds to the silica surface of the magnetic particles in the presence of a chaotropic salt (see flowchart "MagAttract DNA Blood Mini M48 procedure"). DNA bound to the magnetic particles is then efficiently washed. Two different wash buffers are used, followed by a rapid rinse with distilled water, which considerably improves the purity of the DNA. High-quality DNA is eluted in the water provided.

Applications

DNA purified using the MagAttract DNA Blood Mini M48 Kit can be used in a wide range of downstream applications, including: