Next generation Cas9 protein designed to deliver maximum editing efficiency

Introducing Invitrogen TrueCut Cas9 Protein v2, a wild type Cas9 in protein form designed to deliver consistently higher editing efficiency across a range of gene targets and cell types. Based on 30 years of industry-leading innovations, our investment in R&D helps ensure that our products reliably fulfill your research needs:

Consistently high editing efficiency in all tested cell lines including standard, immune, primary, and stem.

Up to 2X higher editing efficiency in difficult targets than the competition

Consistently outperforms the competition

Figure 2. Invitrogen CRISPR genome-editing tools consistently outperform products from other suppliers. Genome editing of multiple gene targets was performed with TrueCut Cas9 Protein v2 and corresponding TrueGuide Synthetic gRNAs. Delivery was achieved using optimized transfection protocols and Invitrogen Lipofectamine CRISPRMAX Transfection Reagent in two cell lines, (A) A549, a human lung carcinoma cell line and (B) MDA-MB231, a human breast cancer cell line. The graphs compare the same experiments using products and recommended protocols from another manufacturer. With the Invitrogen system, cleavage efficiency is improved for low-efficient loci (PRKCG-T1 and CMPK1-T1) and shows consistently greater efficiency when compared to products and protocols from other suppliers, even for challenging loci.

Getting started with CRISPR? Need proven CRISPR validated protocols? Our CRISPR Validated Protocols collection has been developed by our expert R&D team. We have matched each of the validated and optimized protocols with the best products to help reduce trial and error and to accelerate your rate of discovery. These step-by-step CRISPR protocols have been optimized for maximum efficiency, viability, and reproducibility across a broad range of cell types and gene targets. We truly want you to succeed with your CRISPR experiment the first time and have confidence in your data.

Consistently outperforms the competition

Figure 2. Invitrogen CRISPR genome-editing tools consistently outperform products from other suppliers. Genome editing of multiple gene targets was performed with TrueCut Cas9 Protein v2 and corresponding TrueGuide Synthetic gRNAs. Delivery was achieved using optimized transfection protocols and Invitrogen Lipofectamine CRISPRMAX Transfection Reagent in two cell lines, (A) A549, a human lung carcinoma cell line and (B) MDA-MB231, a human breast cancer cell line. The graphs compare the same experiments using products and recommended protocols from another manufacturer. With the Invitrogen system, cleavage efficiency is improved for low-efficient loci (PRKCG-T1 and CMPK1-T1) and shows consistently greater efficiency when compared to products and protocols from other suppliers, even for challenging loci.

Getting started with CRISPR? Need proven CRISPR validated protocols? Our CRISPR Validated Protocols collection has been developed by our expert R&D team. We have matched each of the validated and optimized protocols with the best products to help reduce trial and error and to accelerate your rate of discovery. These step-by-step CRISPR protocols have been optimized for maximum efficiency, viability, and reproducibility across a broad range of cell types and gene targets. We truly want you to succeed with your CRISPR experiment the first time and have confidence in your data.

Our R&D scientists demonstrated that by combining Cas9 protein with CRISPR gRNAs and complexing them with a lipid-based transfection reagent, you can significantly simplify the cell engineering workflow and achieve cleavage efficiencies of up to 85% in many cell lines (Liang et al. 2015).

The Cas9 RNP complex can act immediately after it enters the cell, since transcription and translation are not required. Moreover, the complex is rapidly cleared from the cell, minimizing the chance for off-target cleavage events when compared to vector-based systems.

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High-quality controls play an integral role in the successful development and performance of high-throughput screens. Don't forget to get your controls to help you optimize delivery conditions, maximize editing efficiency, and establish hit selection criteria.