Sapphire700 Stain

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Sapphire700 is a non-specific cell stain that accumulates in both the nucleus and cytoplasm of fixed or dead cells, but not live cells. When used to stain serial dilutions of 431 cells in 96-well plates, Sapphire700 displays linearity of fluorescent signal for higher cell densities, from ~50,000 to ~250,000 cells/well. Simultaneous staining of cells with Sapphire700 and DRAQ5™ (Biostatus, Ltd.) expands the linear range, allowing more accurate normalization of cell number across both low and high cell densities.