The FastStart TaqMan®Probe Master is a ready-to-use, 2x-concentrated master mix that contains all the reagents (except primers, probe, and template) needed for running quantitative, real-time DNA-detection assays, including qPCR and two-step qRT-PCR, in the hydrolysis probe detection format. Use the FastStart TaqMan®Probe Master with any real-time qPCR instrument other than the LightCycler® Instruments.