Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

WB

Use a concentration of 5 µg/ml. Detects a band of approximately 251 kDa (predicted molecular weight: 251 kDa).

Abcam recommends using BSA as the blocking agent.

IP

Use at an assay dependent concentration.

ELISA

Use at an assay dependent concentration.

ICC/IF

1/200. PubMed: 18974869

IHC-Fr

Use at an assay dependent concentration.

IHC-FoFr

Use at an assay dependent concentration.

Target

Function

Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.

Tissue specificity

Detected only in malignant melanoma cells.

Sequence similarities

Contains 15 CSPG (NG2) repeats.Contains 2 laminin G-like domains.

Post-translationalmodifications

O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.

Cellular localization

Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.

ab50009 staining NG2 in an immunoreactive cell (rat hippocampal) by Immunohistochemistry (PFA fixed).Brains were postfixed (2–6 hours) in 4% PFA and placed in 30% sucrose (2 days) and sectioned (20–30 µm) with a cryostat. ab50009 was used at a 1/200 dilution. A goat anti-mouse-Alexa 594, 1/500, was used as the secondary antibody.