Complement Activity (CH50)

Autokit CH50

Intended Use

The Wako Autokit CH50 is an in vitro liposome immunoassay (LIA) for the quantitative determination of total complement activity (CH50) in human serum using an automated procedure.

Method

Liposome immunoassay

Special Features

Fully automated assay

Homogeneous reagent free from precipitation

Stable reagent

Good correlation with gold standard, Mayer's method

One of the most widely used assays for total complement activity is
based on complement-mediated hemolysis of antibody-sensitized
erythrocytes (Mayer's method). This method requires serum dilutions
to achieve 50% lysis of the indicator cells, making this method
complicated and time consuming. In addition, due to the use of
erythrocytes, the reagents are not stable and the assay is difficult to
automate.

Wako has developed an automated homogeneous liposome-based
assay for total complement activity in human serum. The assay
uses a homogeneous population of small-size liposomes (200nm),
which gives stable dispersion. Glucose-6-phosphate dehydrogenase
(G6PDH) is used as the entrapped enzyme which has optimal activity
at a neutral pH. Using this liposomal technology, Wako developed a
fully automated assay system for total complement activity. The speed
and ease of this assay makes it the best choice for your lab.

Applications

Automated Analyzer Applications

To request an automated analyzer application, please email us at: Customer Service. Please include the names of the product and the analyzer/instrument.