Abstract: In this work, we have reported a new approach on the use of stimuli-responsive molecularly imprinted polymer (MIP) for trace level sensing of alpha-fetoprotein (AFP), which is a well know cancer biomarker. The stimuli-responsive MIP is composed of three components, a thermo-responsive monomer, a pH responsive component (tyrosine derivative) and a highly fluorescent vinyl silane modified carbon dot. The synthesized AFP-imprinted polymer possesses excellent selectivity towards their template molecule and dual-stimuli responsive behavior. Along with this, the imprinted polymer was also explored as 'OR' logic gate with two stimuli (pH and temperature) as inputs. However, the non-imprinted polymers did not have such 'OR' gate property, which confirms the role of template binding. The imprinted polymer was also used for estimation of AFP in the concentration range of 3.96-80.0 ng mL-1, with limit of detection (LOD) 0.42 ng mL-1. The role of proposed sensor was successfully exploited for analysis of AFP in real human blood plasma, serum and urine sampleTemplate and target information:protein, alpha-fetoprotein, α-fetoproteinAuthor keywords:Molecular gate, Stimuli-responsive polymer, Fluorescence sensor, molecularly imprinted polymer, Alpha-fetoprotein