Over 85,000 individual constructs were successfully injected and over 420,000 transformants delivered!

Drosophila Embryo Injection Services

We provide high quality Drosophila transgenic service to both research institutions and
companies. We offer you partial to full service ranging from DNA preparation, embryo
microinjection, screening for white+, yellow+, vermilion+ and/or Fluorescence (GFP/RFP/DsRed/YFP/CFP) phenotypes, to balancing crosses. Most importantly, the costs of our Drosophila injection services are more reasonable and affordable compared to that of others. With a large number of facilities and our highly experienced staffs, we are able to handle library-scale injection projects. Our friendly web-based database allows you to track your fly injection status, service history and more. See here for the sample page.

P-element Transformation

The Drosophila P-element transformation usually involves the injection of transposon-based construct
with the white and/or the yellow marker to w1118 or yw host line. We perform injections with your (or our) purified plasmid construct (helper P-element plasmid will be supplied by us unless you prefer to use your own)
and return you the injected lavae, or the stable lines or the balanced lines depending on your chosen service.
For details, please refer to the service page.

PhiC31 integrase-mediated transgenesis systems - P[acman] and FlyC31

PhiC31 integrase-mediated transgenesis systems are based on the site-specific bacteriophage PhiC31
integrase which mediates sequence-directed, irreversible and highly efficient integration between a
bacterial attachment site (attB) and a phage attachment site (attP).
We will inject your purified attB construct into your preferred PhiC31-containing attP
docking site embryos and return you the injected larvae, the stable lines without the
PhiC31 source or the balanced lines (for the FlyC31 system, you may also choose to remove most of the non-necessary sequences by the Cre-mediated excision, leaving ONLY the gene-of-interest flanking with one loxP and one attL site) depending on your chosen service. For details, please refer to the service page and the PhiC31 information page.

CRISPR Genome Editing

The Drosophila CRISPR/Cas9 system are a powerful genome engineering tool that are composed of
programmable, sequence-specific DNA-binding modules associated with a nonspecific DNA nuclease,
which induce DSB (DNA double-strand breaks) that stimulate NHEJ (non-homologous end joining) or
HR (homologous recombination) repairing mechanisms at specific genomic locations. For details, please refer to the service page and the Genome Editing page.