cis-acting mutations that affect rop protein control of plasmid copy number

Moser, David R. and Ma, Doreen and Moser, Catherine D. and Campbell, Judith L.
(1984)
cis-acting mutations that affect rop protein control of plasmid copy number.
Proceedings of the National Academy of Sciences of the United States of America, 81
(14).
pp. 4465-4469.
ISSN 0027-8424.
http://resolver.caltech.edu/CaltechAUTHORS:MOSpnas84

Abstract

A number of pMB1 derivatives provide a trans-acting function that can suppress lethal runaway replication of a temperature-sensitive copy-number mutant of NTP1. Deletion analysis indicates that the region of the pMB1 genome that contains the rop gene is required for this suppression. Mutant derivatives of the temperature-sensitive copy-number mutant plasmid whose conditional lethal phenotype is not suppressed in trans by the region encoding the rop gene have been isolated. These rop-insensitive derivatives contain single nucleotide changes within the RNA I coding region.