A1Acid Glycoprotein Column

In most instances no derivatization is necessary to obtain good enantioseparation. In the case of car-boxylic acids, separation is improved by the addition of ion pairing agents to the eluent. For some basic drugs anionic reagents in the eluent also improves the column efficiency, peak symmetry and resolution.

Covalent derivatization is only seldom used. Acetylation or formylation of the amino and hy-droxyl groups, esterification of the carboxyl group with 2-propanol have been found in some instances to improve the separation.

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