Dear someone,
I have expressed a Drosophila protein in E. coli by using several
strategies (unfused, fused to glutation transferase, and fuse to 6-His
tag) but all of it is unsoluble.
DO YOU KNOW OF ANY WAY TO EXPRESS PROTEINS IN E. COLI THAT ARE SOLUBLE?
THANKS SAVERIO
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Saverio Brogna
University of Cambridge, Departement of Genetics
Downing Street, Cambridge, CB2 3EH, England (UK)
tel +44-223-333970, fax +44-223-333992