Application Note 226:

Use of Eppendorf LoBind® Tubes to consistently prepare and store standard panels for real–time PCR absolute quantifications. Adsorption of DNA to tube surfaces effects accuracy of real–time PCR results. The much higher DNA recovery from Eppendorf® DNA LoBind tubes leads to improved standard curves and subsequently more an accurate interpretation of DNA quantity in samples assayed. Image: Flowchart of DNA standard panels preparation. Genomic DNA stock solution was serial 10–fold diluted in either competitor's low retention or Eppendorf DNA LoBind tubes. 22 µL aliquots of each dilution were stored for 24 hours at –80°C in either competitor's low retention or Eppendorf DNA LoBind Tubes before real–time PCR assay. The PCR efficiency of the samples, which were prepared and stored in Eppendorf LoBind Tubes, was much higher - see Application Note 226 for more details.