Applications/Dilutions

Dilutions

ELISA 1:100 - 1:2000

Immunohistochemistry-Paraffin 3-6 ug/ml

Application Notes

ICC/IF usage is reported in scientific literature (PMID: 26178374). IHC: NBP1-52374 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

Packaging, Storage & Formulations

Alternate Names for Pax7 Antibody

FLJ37460

HUP1

paired box 7

paired box gene 7

paired box homeotic gene 7

paired box protein Pax-7

paired domain gene 7

PAX7 transcriptional factor

Pax7

PAX7B

RMS2

Background

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Alternative splicing in this gene has produced two known products but the biological significance of the variants is unknown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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