RNAprotect Cell Reagent

For immediate stabilization and convenient transport of sorted or cultured cells

Immediate RNA stabilization with no need to remove medium

Cells stable at 30ºC (1 day), 15–25ºC (7 days), or 2–8ºC (4 weeks)

Archiving of cells at –20ºC or –80ºC

RNAprotect Cell Reagent provides immediate stabilization of RNA in sorted or cultured cells. Cells are stabilized at room temperature and can be stored or transported at ambient temperature prior to RNA purification.

RNAprotect Cell Reagent provides immediate stabilization of RNA in sorted or cultured cells. Cells are stabilized at room temperature and can be stored or transported at ambient temperature prior to RNA purification.

Procedure

RNAprotect Cell Reagent immediately stabilizes the gene expression profile in cells. With other technologies, it is necessary to wash or process the cells (e.g., trypsin treatment) prior to adding an RNA stabilization reagent. Changes in expression that may occur during the washing or processing steps are eliminated by adding RNAprotect Cell Reagent directly to cells in culture medium or buffer. Instantaneous RNA stabilization with RNAprotect Cell Reagent can also be extended to pelleted cells not in medium or storage solution. The amount of starting material must not exceed 1 x 107 cells.

Applications

Cells in RNAprotect Cell Reagent can be conveniently stored and transported at ambient temperature before further processing and RNA purification. The RNA can be purified using the RNeasy Plus Mini Kit, or purified simultaneously with DNA from the same cell sample using an AllPrep Kit.