Zheljazkov of Mississippi State University said: "The objective of this study was to estimate podophyllotoxin concentration in American mayapple across its natural habitats in the eastern United States and to identify high podophyllotoxin types that could be used for further selection and cultivar development.

The researchers studied the effect of location, plant nutrient concentration, and phytoavailable nutrients in soil on podophyllotoxin concentration in American mayapple across its natural habitats in the eastern US.

In what is being suggested to be the largest study of its kind, American mayapple leaves were collected from 37 mayapple colonies across 18 states.

The researchers hope that their findings will help develop a Geographic Information System (GIS) map of the genetic resources of American mayapple in the US.

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