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High Resolution Mass Spectrometers role in small molecule studies

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 = q B m Once the ion is trapped, So we can calculate the mass of the ion the magnet bends it into We know the a circular path. Magnetic Field B We measure the frequency m “Light” Ions have a High frequency “Heavy” Ions have a Low frequency

Image Current 0.05 Time-Domain Transient 0.04 0.03 Differential Amplifier 0.02 0.01 0 -0.01 -0.02 -0.03 -0.04 -0.05 0 100 200 300 400 500 600 700 800 Time (ms) The signal is recorded for As the spiraling ion gets near a period of time and then a detect plate, it induces a displayed by the software current that is detected by our instrument.

Ion is now trapped in the magnet. + ION Once we make an ion, we move it into the center of the Magnet. Then, we trap it before it can escape. Electrostatic Barrier Ion sees barrier and is turned back “Gate” shut before the ion escapes From Primer 1998 Marshall.

LTQ Orbitrap Operation Principle 1. Ions are stored in the Linear Trap 2. …. are axially ejected 3. …. and trapped in the C-trap 4. …. they are squeezed into a small cloud and injected into the Orbitrap 5. …. where they are electrostatically trapped, while rotating around the central electrode and performing axial oscillation The oscillating ions induce an image current into the two outer halves of the orbitrap, which can be detected using a differential amplifier Ions of only one mass generate a sine wave signal

The mass spectrum is obtained for a surface sample from a PEG 4000 treated board on the Vasa’supper gun deck Each peak corresponds to a certain molecular mass. The difference between the major peaks is 44 mass units, which corresponds to one -CH2CH2O- entity (n ± 1) in the PEG chain. The three clusters of peaks with mean values of about 615, 1450 and 3920 mass units show that commercial compounds labelled PEG 600, PEG 1500, and PEG 4000 consist of a distribution of molecules, and that the PEG 600 from inside the board has penetrated into the PEG 4000 surface layer.