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Tuesday, May 19, 2015

Product Focus: Microplate Based Snapshot ChIP Assay

Different gene expression depends not only on the binding of specific
transcription factors (TFs) to discrete promoter elements but also on
expression and interaction of co-activators with its associated TFs. The
interactive cofactor complexes allow the functional integration of
multiple TFs and pathways facilitating the formation of distinct
biological programs. Unlike electrophoretic mobility shift assays
detecting the binding of specific TFs present in cell lysates to DNA
sequences in vitro, chromatin immunoprecipitation (ChIP) assay is enable
the analysis of the association of a specific TF or co-factor with
specific promoters in vivo and provides a snapshot of how a regulatory
TF or co-factor affects the expression of a single gene or a variety of
genes at the same time.
To simplify the ChIP assay in a high throughput method, Signosis has
developed a 96-well microplate-based ChIP assay, where all of steps from
IP to DNA elution are handled in microplate wells without sample
transfers in an high throughput way. The assay is an ideal tool for
studying up to 96 protein-DNA bindings in one sample or one protein-DNA
binding in up to 96 samples, or multiple protein-DNA bindings in
multiple samples/treatments.

Benefits:

High Throughput – 96 ChIP experiments can be performed simultaneously in one microplate.

Simple Sample Handling – All steps from IP to DNA
elution are done in a single well, including crosslinking and
fragmentation, without the need to transfer samples.

Affordable – More economical per reaction than conventional ChIP assay.

Principle

ChIP assays reveal complexity for chromatin, transcriptional
regulation, RNA processing and DNA replication. These processes are
regulated by multiple histone modifications that can be mediated by
kinases, methyltransferases, acetyltransferase, ubiquitintransferase and
others. In addition, hundreds of different RNA processing and
transcription factors are involved in these epigenetic changes. As a
result, the regulatory modifications of DNA-bound and accessory factors
may be far greater in number and more complex than previously reported.
Therefore, the ability to simultaneously detect multiple modification
events will enhance our understanding of nuclear processes. The
conventional ChIP method has notable limitations, including
time-consuming procedure, bead-based individual IP and multiple tube
transfers. These limitations result in potential sample loss, cross
contamination and variability. It becomes a bigger problem when multiple
chromatin samples are involved. Signosis provides a microplate-based
chromatin ChIP assay kit, which allows monitoring the nuclear events in a
high throughput way. The kit is optimized to perform all of IP steps
from capturing the protein/DNA complexes to eluting DNA in one single
well.

Stratech Scientific
is a distributor of high quality, competitively priced, reliable
products for research laboratories throughout the UK and Europe. Please
contact us to find out which ranges we can supply in your country.