Associated products

Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

WB

1/2000 - 1/10000. Predicted molecular weight: 37 kDa.

IP

Use at 2-5 µg/mg of lysate.

Target

FunctionConverts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Also has ManNAc kinase activity.

Detection of Human N acetylglucosamine kinase by Immunoprecipitation and Western Blot.
Whole cell lysate from HeLa at 1 mg for IP, 20% of IP loaded.
ab95182 was used for IP at 3 mcg/mg lysate and 1ug/ml for wb (lane 2)
N acetylglucosamine kinase was also immunoprecipitated by another rabbit antibody (lane 1) and control IgG (lane 3).
Detection: Chemiluminescence with exposure times of 30 seconds.

References for Anti-N acetylglucosamine kinase antibody (ab95182)

ab95182
has not yet been referenced specifically in any publications.

Publishing research using ab95182? Please let us know so that we can cite the reference in this datasheet.