FEATURED NEW PRODUCTS

New Tools for Vascular Studies―ABfinity™ Recombinant Antibodies for P-Selectin

what they are P-selectin is a 140 kDa type 1 transmembrane glycoprotein stored in the Weibel-Palade bodies of endothelial cells, as well as in the α-granules of platelets. This protein can rapidly be brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate. Researchers have found that P-selectin, which is encoded by the SELP gene, contributes to the adverse vascular processes that promote cognitive impairment in individuals with cardiovascular disease. Our broad antibody portfolio now includes ABfinity™ recombinant monoclonal and oligoclonal antibodies for P-selectin.

what they offer

Consistent lot-to-lot results

Minimize the need to revalidate working antibody dilutions each time you order

how they work ABfinity™ antibodies are manufactured by transfecting mammalian cells with high-level expression vectors containing immunogen-specific antibody heavy- and light-chain cDNA. This production process offers consistent lot-to-lot antibody performance. ABfinity™ oligoclonal antibodies are a mixture of recombinant monoclonal antibodies. This combines the improved signal strength of a polyclonal antibody with the highly reproducible results you get from ABfinity™ monoclonal antibodies.

what they are Rat anti-mouse CD90 (clone G7) and CD90.2 (clone 30-H12) monoclonal antibodies are now available as FITC, phycoerythrin (PE), and allophycocyanin (APC) conjugates.

what they offer

Flexibility—3 colors available for use with a blue or red laser

Convenience—packaged in 25 µg size

Affordability—average price is 50% less than other antibodies

how they work Part of the immunoglobulin superfamily, CD90/Thy-1 is a GPI-anchored cell surface receptor expressed on thymocytes, peripheral T lymphocytes, some intraepithelial T lymphocytes, and neurons of all mouse strains, whereas the CD90.2/Thy-1.2 alloantigen is expressed on all thymocytes, peripheral T lymphocytes, and some intraepithelial T cells of most mouse strains. The CD90/Thy-1 clone G7 antibody recognizes the Thy-1.1 and Thy-1.2 alloantigens (Thy-1 epitope region A). The CD90.2/Thy-1.2 clone 30-H12 antibody recognizes the mouse CD90.2/Thy-1.2 alloantigen.

what it is The Rat Cytokine Magnetic 10-Plex Panel for the Luminex® platform is designed to simultaneously quantify 10 rat cytokines in serum, plasma, and tissue culture supernatant samples. The magnetic beads facilitate automation and easier wash steps. The panel is suitable for use with the Luminex® 200™, FLEXMAP 3D®, and MAGPIX® systems.

More from your precious samples―measure multiple proteins in the same sample

Fast and easy protocols—perform your assays and analyze your data typically in less than one day

how it works The assay has a 3.5-hour incubation time and is similar to an ELISA workflow. Magnetic beads covalently bound to different antibodies can be mixed in the same assay in a 96-well microplate format. At the completion of the sandwich immunoassay, beads can be read using a Luminex® instrument and xPONENT® software to distinguish bead color (analyte) and assay signal strength (PE fluorescence intensity).

NEW APPLICATIONS

The CyQUANT® Direct Cell Proliferation Assay is based on a cell-permeant DNA-binding dye that is used in combination with a background suppression reagent. DNA content is highly regulated, and cell number estimates obtained with the CyQUANT® Direct assay are very accurate. As a result, the CyQUANT® Direct assay is commonly used as a fluorescence-based readout for cell proliferation and cytotoxicity. The addition-only, no-wash assay format renders the CyQUANT® Direct assay suitable for high-throughput screening (HTS) applications.

A new protocol provides guidance for using the CyQUANT® Direct assay with mammalian cells in HTS applications. Because the CyQUANT® Direct assay readout involves highly sensitive fluorescence measurement of cellular DNA content, the protocol provides recommendations for proper plate setup and handling that are essential for optimal assay performance.

Even distribution of the cells in the wells is important for optimal CyQUANT® Direct assay performance. (Left) Cells stained with the CyQUANT® Direct Cell Proliferation Assay that were not set up properly for high-throughput screening and as a result grew unevenly during a cell proliferation time course. (Right) Cells that were set up correctly according to protocol recommendations, resulting in even distribution across the well.

From the Bench

Rapid Click Chemistry Labeling Compatible With Live Cells

The copper-catalyzed azide–alkyne cycloaddition (CuAAC) is commonly used for biomolecule conjugation but poses toxicity problems when used to label molecules in living cells because of the reactive oxygen species generated by the relatively high levels of CuI needed for reaction efficiency. Uttamapinant and colleagues have developed a new azide reaction partner—a picolyl azide, containing an internal CuI ligand—that maintains an increased CuI concentration in the region of the reaction site only. After only 20 minutes of incubation time in the presence of a noncytotoxic concentration of CuSO4 (50 µM), this picolyl azide delivered improved labeling of the target biomolecules in live cells (up to 25-fold better than the alkyl azide), and was even effective for labeling delicate neurons. In further experiments to investigate its utility for visualizing RNA and proteins metabolically labeled with 5-ethynyl uridine and L-homopropargylglycine, respectively, the picolyl azide delivered ~2-fold improvement in signal intensity compared to the alkyl azide form.