Gracilaria is a genus of red algae (Rhodophyta) which is an important raw material for agar industry and some other industries. Nutrient is important environmental factors in seaweed growth. Gracilaria has high potential for absorbed of nutrient in sea water. The following work is devised on the basis of impact of variable concentrations of nitrogen and phosphorous which are 0, 20/2, 50/5, 120/12 and 300/30 µM. The factors determined to be affected were protein soluble, growth and pigments in red seaweed Gracilaria manilaensis. Results stated that concentration alteration of N and P affected the specific growth rate, total soluble proteins and chemical composition (concentrations of chlorophyll a, phycoerythrin (PE), phycocyanin (PC)). N and P concentration was increased, the growth rate of Gracilaria manilaensis were increased 2.97± 0.26%d-1, in 0 concentration of ammonium to 5.72± 0.19%d-1 in 300/30 µM . One -way ANOVA showed significant differences in growth rate between treatments and cultivation time (P<0.05). Growth rates were 50% higher in 300/30 N/P concentration compared to without nutrient treatments. Similarly, PE, PC and Chl a were increased and highest value were found at N/P concentration of 300/30 µM. Growth rate of G. manilaensis under different concentrations of N and P was strongly dependent on the concentrations of inorganic concentration N and P.