HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF AMLODIPINE AND ATENOLOL AND PHARMACEUTICAL DOSAGE FORM

A Simple, specific and sensitive an isocratic Estimation by RP-HPLC analytical Method were developed and validated for the quantification Amlodipine. And Atenolol Quantification was achieved by by using the mobile phase (55 volumes of mixed phosphate buffers ph-4.0: 45 volumes of acetonitrate sonicate for 10 mins for removing gases). Inertsil BDS C18 250×4.6mm ID, 5?m Particle size was used as stationary phase. The flow rate was 1.0 ml/min. Measurements were made at a isobestic wavelength of 220nm. The average retention times for Atenolol And Amlodipine was found to be 6.060 & 2.593 min. The proposed method was validated for selectivity, precision, linearity and accuracy. The assay methods were found to be linear Atenolol And Amlodipine from 60-140?g/ml & 6-14 ?g/ml for respectively. All validation parameters were within the acceptable range. The developed methods were successfully applied to estimate the amount of Atenolol And Amlodipine.