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Creative Data Solutions (CDS) is a Vanderbilt Shared Resource and has extensive experience in providing effective and robust solutions to challenges pertaining to research data using modern informatics and bioinformatics approaches.

Frozen embryo rederivation. This service
can usually be scheduled within two weeks of receiving
cryopreserved embryos and is the most efficient means of rederiving
a line. Frozen embryos will be accepted at the 2- to 8-cell
stage. The embryos will be thawed according to the protocol
used by the lab that froze the embryos and washed numerous times
before being transferred into a recipient female. Pups will
be born approximately 19 days later. At 3 weeks of age, the
PI will receive tail biopsies for genotyping, which must be
completed before serological testing. Serological testing is
routinely performed at 6 weeks of age. When these results
have been obtained, usually by 7 weeks of age, the mice are moved
to the investigator’s room for housing.

Fresh embryo rederivation. This approach
is similar to the frozen embryo rederivation above and is also an
efficient means of rederiving a line. However, the
performance of this service requires more planning and
communication between the lab sending the embryos and the
Vanderbilt Genome Editing Resource in order to prepare for the
embryo transfer surgeries. Embryos can be shipped between the
2-cell stage to the morula/blastocyst stage for transfer. The
pups will be born approximately 19 days after the embryo transfer.
Again, at 3 weeks of age, the PI will receive tail biopsies
for genotyping, which must be completed before serological
testing. At 6 weeks of age, serological testing is done and
by 7 weeks of age, the mice are moved to the investigator’s room
for housing.

In VitroFertilization.
Another option for rederivation is in vitro fertilization (IVF)
using frozen sperm. Performance of IVF and embryo
transfer usually requires about a month of planning prior to the
experiment. The day following the IVF procedure, the
fertilized embryos will be washed and prepared for an embryo
transfer into appropriate recipients. As above, once the
embryos are transferred, pups will be born approximately 19 days
later. At 3 weeks of age, the PI will receive tail biopsies
for genotyping, which must be completed before serological
testing. At 6 weeks of age, serological testing is done and
by 7 weeks of age, the mice are moved to the investigator’s room
for housing.