ABSTRACT
Diosgenin is a steroidal saponin present in various medicinal plants.one of the novel bioactive compound as
Diosgenin, because of its versatility, and it grabs the attention of researchers to explore its wide pharmacological
activities. Tribulus terristris is a natural herb which is at a high rate of medicinal activity according to the present novel
research works. Accordingly, Tribulus terristris have a major content of saponin which results in positive treatment of
various disorders. Between the saponin content of tribulus herb, diosgenin is one of the major saponin present in it. In
the present work, saponin is extracted from the T.Terristris with Ethanol and in turn leads to acid hydrolysis for
diosgenin separation with 5 % HCl. The diosgenin extraction is performed by direct crude acid hydrolysis and
Ethanolic saponin acid hydrolysis for better yield of diosgenin as 92.57 % and 59% respectively. The estimation of
Diosgenin extracted is compared to the standard (0.75) and Rf values of the samples are 0.74,0.61. The diosgenin
sample extracted from Tribulus is rich of medicinal effects, so as natural drug can be used in our daily lives with
minute side effects.