In this paper, we report, for the first time, sonoluminescence (SL) images of living body (nude mouse) injected with and without chemical agents under weak ultrasound field. We found FCLA (Fluoresceinyl Cypridina Luminescent Analog), a chemiluminescent analytic agent, could enhance SL and can work in vivo. We also makes use of sonosensitization to produce tumor-selective oxygen radicals that can react with FCLA to emit chemiluminescence. By detecting this luminescence, we obtained a clear diagnostic image. This novel method proposed by our group has potential application in clinics for tumor early-stage diagnosis.