PhosphoSitePlus® Resource

PTMScan® Phospho-Tyrosine Mouse mAb (P-Tyr-100) Kit #5636

Motif analysis was done using all tyrosine phosphopeptides generated from studies in the published report by Rikova, K.A., et al. (2007) Cell 131, 1190-1203. The Motif Logo, drawn from 4160 non-redundant sites, shows the amino acid distributions around the sites recognized by Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411. The motif analysis shows that this antibody is a general phospho-tyrosine antibody and does not have amino acid preferences at other positions.

Gallery: PTMScan® Phospho-Tyrosine Mouse mAb (P-Tyr-100) Kit #5636

Motif analysis was done using all tyrosine phosphopeptides generated from studies in the published report by Rikova, K.A., et al. (2007) Cell 131, 1190-1203. The Motif Logo, drawn from 4160 non-redundant sites, shows the amino acid distributions around the sites recognized by Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411. The motif analysis shows that this antibody is a general phospho-tyrosine antibody and does not have amino acid preferences at other positions.

Product Usage Information

Cells are lysed in a urea-containing buffer, cellular proteins are digested by proteases, and the resulting peptides are purified by reversed-phase, solid-phase extraction. Peptides are then subjected to immunoaffinity purification using a PTMScan® Motif antibody conjugated to protein G agarose beads. Unbound peptides are removed through washing, and the captured PTM-containing peptides are eluted with dilute acid. Reversed-phase purification is performed on microtips to desalt and separate peptides from antibody prior to concentrating the enriched peptides for LC-MS/MS analysis. CST recommends the use of PTMScan® IAP Buffer #9993 included in the kit. A detailed protocol and Limited Use License allowing the use of the patented PTMScan® method is included with the kit.