Special tissue culture methods by Roland M Nardone(
Visual
)2
editions published
in
1984
in
English
and held by
2 WorldCat member
libraries
worldwide
The program examines two special tissue culture techniques: primary culture and organ culture. Provides information for primary
care specialists working with laboratory personnel to diagnose viral infections

In Vitro Studies of Neurotoxic Substances: The Effect of Organophosphates and Acrylamides(
Book
)1
edition published
in
1982
in
English
and held by
1 WorldCat member
library
worldwide
The toxicity of acrylamide, n-methylacrylamide, and crotonamide as well as the organophosphates mipafox, leptophos, paraoxon,
EPN, OMPA and DFP were studied in order to see whether or not in vivo-in vitro neurotoxicity correlations could be established.
The in vitro systems employed were the mouse neuroblastoma cell line NIE-115 and the chick brain, either as cell aggregate
cultures or organ culture. In both the neuroblastoma cell culture and chick brain cell/organ culture systems, acrylamide was
the most toxic. The ranking of acrylamide, n-methylacrylamide and crotonamide paralleled the ranking reported in vivo. The
end-points which showed this ranking included cell viability and neuron-specific enolase activity and aggregate formation
by dissociated brain cells. The organophosphate studies emphasized their effect on neurotoxic esterase activity. A model in
vitro test has been developed for the evaluation of neurotoxic esterase effects. The test is based on the hen brain assay
test developed by Johnson, as a predictor of delayed neuropathy. (AUTHOR)

In vitro studies of neurotoxic substances by Roland M Nardone(
Book
)2
editions published
in
1985
in
English
and held by
1 WorldCat member
library
worldwide
An in vitro strategy for the evaluation of the neurotoxic potential of chemicals was developed. A number of investigations
were undertaken to validate portions of the proposed test battery. The components of the test battery which were used are
as follows: neuroblastoma cell lines and chick brain cell and organ culture tester cells; acetylcholinesterase, neuron-specific
enolase, and neurotoxic esterase activity, and acetylcholine receptor number as neuronal end-points; acrylamides and organophosphates
as model chemicals. The research also led to the developement of an in vitro alternative for the hen brain assay for neurotoxic
esterase (which is predictive of delayed neuropathy), the validation of the use of phenyl 4-butyrate (which is commercially
available) as a substitute substrate for phenyl valerate (which must be custom synthesized) in the neurotoxic esterase assay,
and the development of an in vitro method for the evaluation of the efficacy of anti-organophosphate chemical defense agents,
such as an anti-paraoxon monoclonal antibodies. Studies on acetylcholine receptors indicate that the receptor nubmer in chick
brain cultures increases with time in culture as well as with exposure to organophosphates

The effect of ionizing radiation on the metabolism of Strain L by Roland M Nardone(
Book
)1
edition published
in
1964
in
English
and held by
1 WorldCat member
library
worldwide
Included in this final report is a brief recapitulation of research described in earlier reports as well as the results of
the research completed during the terminal year of the contract period. Throughout the entire contract period experiments
were performed in an attempt to better understand the effect of ionizing radiation on the survival, growth, cell division
and metabolism of a mammalian cell grown in vitro. Most of the radiation work done prior to 1960 (when this project was initiated)
revolved around whole mammals. The work which involved cells was, for the most part, quite diffuse and varied in that a variety
of cell types, radiation dose rates, and pre-and post- irradiation manipulations were used. Thus, it was necessary to become
engaged at the beginning of this research in a broad spectrum of physiological studies in order to establish in general terms
the nature of a radiation syndrome for the cell we selected for our experiments. Once this was done, studies on RNA metabolism,
aerobic respiration, and cell cycle dynamics were emphasized