Accuracy profile validation of a new analytical method for butane measurement using headspace-gas chromatography-mass spectrometry.

Details

Accuracy profile validation of a new analytical method for butane measurement using headspace-gas chromatography-mass spectrometry.

Journal

Journal of Chromatography. B, Analytical Technologies In the Biomedical and Life Sciences

Author(s)

VarletV., SmithF., AugsburgerM.

ISSN

1873-376X (Electronic)

ISSN-L

1570-0232

Publication state

Published

Issued date

2013

Volume

913-914

Pages

155-160

Language

english

Notes

Publication types: Journal Article Publication Status: ppublish

Abstract

The aim of our study was to provide an innovative HS-GC/MS method applicable to the routine determination of butane concentration in forensic toxicology laboratories. The main drawback of the GC/MS methods discussed in literature concerning butane measurement was the absence of a specific butane internal standard necessary to perform quantification. Because no stable isotope of butane is commercially available, it is essential to develop a new approach by an in situ generation of standards. To avoid the manipulation of a stable isotope-labelled gas, we have chosen to generate in situ an internal labelled standard gas (C(4)H(9)D) following the basis of the stoichiometric formation of butane by the reaction of deuterated water (D(2)O) with Grignard reagent butylmagnesium chloride (C(4)H(9)MgCl). This method allows a precise measurement of butane concentration and therefore, a full validation by accuracy profile was presented.