C-C motif chemokine 21 (CCL21)

Links to databases

Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

NOTE: The calibrator curve below shows the performance of the assay with the estimated sensitivity and dynamic range parameters indicated. These curves are generated during the assay validation process using recombinant antigens. Please note that when analyzing biological samples the data generated will be given in the form of relative quantification (NPX values) and cannot be converted to absolute protein concentrations. For more info about NPX measurements, please visit our FAQ page.

Analytical Measuring Range

Please note: the technical data reported below refers to the measured value in the in vitro validation assays. Since the Development panel uses human samples diluted 100-fold, a multiplication factor of 100 should be applied when comparing the addressable biological concentration to this validation data.

LOD (pg/mL)

15

LLOQ (pg/mL)

15

ULOQ (pg/mL)

7812

Hook (pg/mL)

250000

Range (logs)

2.7

Calibrator curve for validation data (generated in multiplex)

Precision

Within run precision Coefficient of Variation (CV)

10%

Between run precision Coefficient of Variation (CV)

7%

Precision (repeatability) is calculated from linearized NPX values over LOD.

Biomarker Validation Data

Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents. To download or to learn more go to the Data Validation page.