HPLC systems

On 4 Jan 1996 aandonov at HPB.HWC.CA wrote:
>> Hi netters!
> We are looking to by a high pressure liquid chromatography
> system mainly for protein purification.As we do a lot of PCR
> we may use it with DNA too. The first name to come is Pharmacia,s
> FPLC.We need just the basic thing, no fancy automation etc.
> BioRad also has a HPLC sys. Any users with good experience,please
> SHARE it.
>> Anton Andonov,PhD
> Laboratory Centre for Disease Control
> Ottawa,Ontario
>
Forget to use such a system for DNA purification. It is not worth since
classical molecular biology techniques and kits are cheaper and faster.
For purification of proteins go for Pharmacia. I have four different
systems in my lab. Both from Pharmacia are the most used: FPLC and Smart.
Jose M. Bautista
RPMS-London