I have assessed the specificity of a particular monoclonal antibody against a virus by immunofluorescence. I'd like to further validate it by testing it by IHC. Therefore I'd like to infect cells in ...

Haemolytic disease of the newborn can result from Rhesus incompatibility in utero. In this disease a Rh-ve mother becomes exposed to the antigens of a Rh+ve foetus by fetomaternal haemorrhage causing ...

I'm physicist by training, so please excuse me if I don't use the proper terminology.
I think there is a way to make a sensor that detects if a single antibody has caught something from the flow. So ...

I need to detect a FLAG tagged protein on a western blot and have to order an antibody. I'm not expecting to see a massive amount of my protein. To keep analysis time to a minimum I am considering a ...

After reading about complement pathway and the complement components, I was wondering, does the C1q component attach to the Fab fragment or to the Fc fragment? The pictures in Janeway's Immunobiology ...

Recently, I have been reading Janeway's immunobiology and had a question on immunoglobin A. I read that IgA activates the complement pathway using the Fab fragment of the IgA. How does IgA do that? I ...

I was reading about Fab fragment and about using them to fight of viral infections. It seems that the Fab attaches to the viral receptors, which stops the viruses from attacking the cells. It seems ...

In a previous post of mine, I asked how to supress the creation of anti-antibodies in vivo. In the answer, it was mentioned that site-directed mutagenesis could be used. Currently, I can't find mcuh ...

Is there a way to inhibit an antibody response to a specific antigen using immunosupression? I am interested in reducing the anti-antibody formation to animal antibodies such as murine antibodies in ...

Complementarity determining regions (CDRs) are part of the variable chains in immunoglobulins (antibodies) and T cell receptors, generated by B-cells and T-cells respectively, where these molecules ...

I'm just curious as there was a bit controversy around this topic. It is possible for person with AIDS be negative for HIV antibodies?
As of 1989, the CDC reported that 5% of all U.S. AIDS patients ...

What is a protective epitope? An epitope is basically a part of antigen. So does it mean that when the epitope combines with an antibody, it helps in the functioning of the antibody instead of going ...

I wonder why Rhesus conflict can happen during pregnancy and mother can make antibodies against Rh protein (I think the correct name is D protein), but it doesn't happen if mother has different ABO ...

Dont tell me the "function" of IgE is to cause allergy !
In whatever texts I have seen it is written that IgE is important to cause allergies but what is the beneficial function of IgE ? Why was it ...

Many women cannot or will not feed their children with breast-milk. It is my understanding breast-milk has many advantages over formula. Some psychological like oxytocin and some physiological like ...

I'd like to clear something up about antibodies that I'm not sure I've understood in the articles I've read. Looking at concepts such as "affinity maturation", "monovalent antigens" and "polyvalent ...

The amplification on vector transgenic microorganisms and the subsequent purification does not seem that complicated. Also, what are the processes that biotechnological industries follow, in detail, ...

I'm learning about antibodies. As I understand it, antibodies detect stranger cells/bacterial/viruses by the molecules present in their membranes. In cancer cells, the cancer cell have produce some ...

A person wih blood group O is called a Universal Donor. Well, his plasma contains antibodies A and B. During blood donation, if blood group O is given to a person with blood group A (since blood group ...

I want to experimentally look at the behavior of antibodies. To do so, I need to be able to bind these antibodies to a substrate. Does anybody know of a good substrate to use that antibodies bind to?
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Antibodies or immunoglobulins are proteins made ​​by the immune system in response to alien(!) molecules. Each antibody binds to its specific antigen. This great diversity and specificity is cause of ...

I have recently peformed an ELISA (Enzyme-linked immunosorbent assay), but I still have some questions, let me first outline what I did:
We had a number (20) of tubes containing fake 'bodily fluids' ...

Antibodies have the ability of recognising highly specific peptide sequences and bind it at their antigen-binding site.
This ability is harnessed as a tool in research to purify target structures in ...

In a Flow Cytrometry, one can add an Isotype Control Antibody to allow another antibody to bind more specific to the cells.
My question is, how can the Isotype Control Antibody add specificity to the ...

I am wondering if you were theoretically able to get vaccinations or antibodies for any and every diseases and/or illness, would there be a limit to how many you can get and keep in your body at one ...