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U.S. 4,464,254: PATENT

Abstract

A method of producing a debris-free stool supernatant suitable for use with automatic instrumentation is disclosed. A stool sample is briefly centrifuged after mixing with appropriate buffer and/or other diluent. The stool supernatant is then filtered using a serum filter device such as that designed primarily to remove fibrin and particulate matter from serum. The method is applicable to fresh stool samples or to stool samples in various transport media or preservatives.

Copyright

October 2002 Remel Inc.

Country

United States

Language

English (United States)

This text was extracted from a Microsoft Word document.

This is the abbreviated version, containing approximately
55% of the total text.

Preparation of a Debris-Free Stool
Supernatant

Suitable for Use with Automated
Instruments

Introduction

� � � � � � � � � � � Although
enzyme-linked immunoassay (EIA) products applicable to stool specimens are
commercially available, stool samples are routinely incompatible for use with
automated equipment such as EIA plate or strip washers and EIA plate processors
due to occlusion of aspiration probes or tubing with debris common to such
specimens.� The problem of occlusion by
specimen debris likewise affects other types of automated processes besides
EIA.

� � � � � � � � � � � Disclosed
below is a method of producing a debris-free stool supernatant suitable for use
with instrumentation and considered “automation compatible”.� The method is applicable to fresh untreated
stool samples, stool samples in preservative or fixative solutions, or stool
samples in other solutions such as microbial transport medium.

Procedure

1.
Prepare the stool sample for testing according to the
“Instructions for Use” information provided with the particular diagnostic test
kit to be performed.

2.
Using standard-sized disposable tubes (10.25 mm, 13 mm, 16 mm,
etc.), prepare the desired dilution of a stool sample in the appropriate buffer
or solution for the particular test to be performed.

3.
Centrifuge the diluted sample at 1500 rpm for 1 – 5 minutes.

4.
Select a disposable serum filter device, designed to remove
fibrin and particulates from the serum portion of centrifuged blood sampl...