A double antibody enzyme linked immunosorbent assay for identification of thermostable muscle antigens of autoclaved meat samples is described. The assay differentiates heterologous thermostable muscle antigens from homologous at P 0.001. In model meat mixtures, the assay detects adulterants at the level of 1% at p0.001 even in phylogenetically related species such as buffalo and cattle.

A double antibody enzyme linked immunosorbent assay for identification of thermostable muscle antigens of autoclaved meat samples is described. The assay differentiates heterologous thermostable muscle antigens from homologous at P 0.001. In model meat mixtures, the assay detects adulterants at the level of 1% at p0.001 even in phylogenetically related species such as buffalo and cattle.

Sera from various wildlife ruminants coexisting in the same habitat with cattle had hemagglutiation-inhibition antibodies as high as those in cattle.Sera from wildebeest, Kongoni, Thomson's gazelle, eland and African buffalo had titres ranging from 64 to 128.Zebra, Bushbuck, Warthog and Oryx were negative to the H I test.It was suggested that some of the wild ruminants with high titres could be possible reservoirs of para-influenza 3 virus.

Six Topi (Damaliscus korrigum) from the Masai Mara area of Kenya were examined. All had lungworm nodules. The nodules were generalized in distribution among the lung lobes. These nodules were essentially hepatized lung tissue with adult worms, larvae and eggs with marked cellular reaction accompanying the infection. The cell types were mostly lymphocytes, plasma cells, macrophages, giant cells and eosinophils. The worms were identified as protostrongylus africanus.

Previous study by our group (Genigiorges V. Symposium W.A. WF.H P 473 1969; Genigeorgis et al App Microbiology 21: 934: 1971) have indicated that the probability of one staphylococcus cell initiating growth in foods is dependent on the magnitude of the various chemicals parameters of the food and the environment. The study has indicated that, as the environment becomes more inhibitory, a heavier level of staphylococcal contamination is required to initiate growth.