Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

23 kDa (note)

Positive Controls

293T , A431 , HeLa , HepG2 , HT-29 , Mouse liver

Form Supplied

Liquid

Concentration

0.45
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Affinity purified by Protein A.

Specifications: HSP27 antibody

Full Name

heat shock 27kDa protein 1

Product Description

Rabbit Polyclonal antibody to HSP27 (heat shock 27kDa protein 1)

Background

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq]

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody (GTX101145) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of HSP27 protein from HeLa whole cell extracts using 5 μg of HSP27 antibody (GTX101145).Western blot analysis was performed using HSP27 antibody (GTX101145).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody (GTX101145) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of HSP27 protein from HeLa whole cell extracts using 5 μg of HSP27 antibody (GTX101145).Western blot analysis was performed using HSP27 antibody (GTX101145).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.