7. IVF and IVC were performed in an atmosphere of 5% CO2 and 95% air at 37°C.

8. Oviduct flushing was conducted according to the method of Horgan et al. [13].

9. Immediately after the administration of Gonatropin to female mice, they were mated with males of the same strain. On day 1.5 after mating, the female mice were euthanized for collection of two-cell stage embryos.

10. IVF was conducted using a modified method of Toyoda et al. [35,36].