Proteins of this family play an important role in inducing nonspecific resistance against a broad range of viral infections. They also affect cell proliferation and modulate immune responses. Produced by peripheral blood leukocytes and lymphoblastoid cells, IFNα is an acid stable molecule that signals through IFNα/βR, which is also used by IFNβ. Both IFNs have similar anti-viral activity and regulate expression of MHC class I antigens. Recombinant human IFNβ is an 20.0 kDa protein containing 166 amino acid residues. Due to glycosylation, IFNβ has an approximate MW of 22.3 kDa based on SDS-PAGE gel and Mass Spectrometry.

Catalog #:

001-HIFNb

Source:

CHO cells (Chinese Hamster Ovarian cells)

Stability:

The lyophilized protein is stable for at least 2 years from date of receipt at -200C.
Reconstituted IFN-β is stable for at least 3 months when stored in working aliquots with a carrier protein at -200C. Avoid repeated freeze/thaw cycles.

Purity:

Greater than 98% by SDS-PAGE and HPLC analyses.

Endotoxin Level:

Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Biological Activity:

The biological activity of human IFN-β was based on analysis using the WISH cell line with VSV as the challenge virus. The specific activity was determined to be >5 x 10 8 units/mg.