Tools & Reagents

To help make CRISPR genome engineering accessible to other researchers, we share our protocols and reagents whenever possible. We also maintain a list of computational tools that may be useful in setting up CRISPR experiments, analyzing data, and more.

Protocols

Detailed protocols for an assortment of techniques related to CRISPR-Cas9 gene editing are available for free at protocols.io. Browse IGI-generated protocols, including:

Cas9 protein expression and purification

In vitro transcription of guide RNAs

Cas9 RNP nucleofection

Genomic DNA extraction and PCR

Re-amplification of CRISPRi and CRISPRa libraries

Protocols used by our CRISPR screening facility can be found here, including:

Genome Editing in Animals. Edited by Hatada I. Methods in Molecular Biology (2017).
Covers methods for editing genomes of many different animals, including insects, fish, and mammals.

Plasmids & Libraries

A variety of plasmids and pooled guide RNA libraries for CRISPR-mediated genome manipulation generated by IGI member labs and others can be acquired through AddGene. Our CRISPR screening facility also distributes certain gRNA libraries.

Cell Lines

Published CRISPR interference, activation, or knockout cell lines may be provided upon request from the cell culture facility at UC Berkeley. Browse available lines here and contact Alison Killilea to learn more: ankillilea (at) berkeley.edu.

Guide RNA Design

*You may wish to use a tool designed specifically for the organism you want to edit.*