Violet Red Bile Glucose Agar

Violet Red Bile Glucose Agar is used for the enumeration of Enterobacteriaceae in foods in a laboratory setting. Violet Red Bile Glucose Agar is not intended for use in the diagnosis of disease or other conditions in humans. Violet Red Bile Glucose Agar conforms to Harmonized USP/EP/JP Requirements.

The Enterobacteriaceae group includes lactose-fermenting coliform bacteria, nonlactose-fermenting strains of E. coli, and nonlactose-fermenting species such as Salmonella and Shigella. When examining certain foods, it is important to detect Enterobacteriaceae rather than coliform bacteria. Enterobacteriaceae are glucose-fermenting bacteria. Mossel et al. modified Violet Red Bile Agar, which contains lactose, by adding glucose to improve recovery of Enterobacteriaceae. Further research by Mossel et al. demonstrated that lactose could be omitted, resulting in the formulation known as Violet Red Bile Glucose Agar.

Violet Red Bile Glucose Agar conforms to Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP).

Formula

Liter

Enzymatic Digest of Gelatin

7 g

Yeast Extract

3 g

Dextrose

10 g

Bile Salts

1.5 g

Sodium Chloride

5 g

Neutral Red

0.03 g

Crystal Violet

0.002 g

Agar

*13.5 g

*10-15 g according to gel strength
Final pH: 7.4 ± 0.2 at 25°CFormula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

Suspend 40 g of the medium in one liter of purified water.

Heat with frequent agitation and boil for one minute to completely dissolve the medium.