Flow Cytometry (1:100)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Purity Detail:

Protein A affinity purified.

Formulation:

Liquid. In PBS containing 0.09% sodium azide.

Handling:

Avoid vigorous centrifugation and vortexing. Do not freeze. Protect from light.

Shipping:

Blue Ice Not Frozen

Long Term Storage:

+4°C

Scientific Background:

In response to adverse changes in their environment, cells from many organisms increase expression of a class of proteins referred to as heat shock or stress proteins. Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30 kDa. Studies on estrogen-induced proteins in breast cancer cells suggest a direct relationship between elevated stress protein expression and neoplasia. Hsp27 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g., phorbol esters) and calcium ionophores1 and elevated levels of Hsp27 correlate with endometrial adenocarcinomas. Interestingly, the regulation of human Hsp27 by estrogens and heat shock resembles the ecdysteroid and heat shock activation of the drosophila Hsp20 genes. This antibody reacts with both the phosphorylated and the non-phosphorylated forms of Hsp25.