I had a problem cloning a PCR product like that. It turned out that my
recovery procedure for the fragment(geneclean) made the sample
refractive to digestion with my enzymes. I switched isolation methods (to
beta-agarase) and never had any problems again. A question though--- you
are using a TA vector if the fragment was amplified with Taq right?
Helen McBride
University of Utah
Grad Student
Dept. of Onc. Sci.
helen_mcbride at hlthsci.med.utah.edu
"Where the telescope ends, the microscope begins, which of the two has
the grander view?" Victor Hugo