Abstract:Linalool is one of the most important volatile compounds in aromatic plants. The nerolidol/linalool synthase gene is an important regulatory gene for linalool biosynthesis. In this study, based on the transcriptome results of jasmine petals, we cloned the full length cDNA and promoter sequence of NEL/LINS using RT PCR and Genome Walking Technique, respectively. The content of linalool was detected by GC MS, and the expression level of JsNEL/LINS during the opening process and after treatments of IAA, GA, ABA and MeJA, which provided a theoretical basis for further study of the role of JsNES/LINS gene in the biosynthesis regulation of jasmine linalool. The results showed that： (1) the full length cDNA of JsNEL/LINS (GenBank accession number：MH513857) was 1 843 bp，contained 1 755 bp open reading frame (ORF)， encoding a protein of 584 amino acids, and belonged to hydrophilic and stable protein. The results of NCBI BLAST showed that JsNES/LINS had 56%，54%，54% and 50% similarities with Olea europaea OeNES， Antirrhinum majus AmNES/LINS1，AmNES/LINS 2 and Camellia saluenensis CsLINS, respectively. (2) The result of subcellular localization showed that JsNEL/LINS was located on the cytoplasm. 1 141 bp promoter sequence of the JsNES/LINS was isolated, and the result predicted that it contained several cis acting elements involved in plant hormones and light response，indicating that the expression of JsNEL/LINS was regulated by plant hormones and light. (3) The results of GC MS analysis showed that the relative content of linalool was the lowest when jasmine was not opened at 18:00, and reached the maximum when it was half open at 22:00, and then decreased. (4) The analysis of qRT PCR showed that the relative expression level of JsNES/LINS was consistent with the change of the relative content of linalool compounds during the flowering process. The expression of JsNES/LINS was induced by IAA, GA, ABA and MeJA, and the induction of MeJA was the most obvious, and the induction from high to low were MeJA>GA>ABA>IAA. It is speculated that JsNES/LINS plays an important role in the biosynthesis of linalool compounds and is regulated by plant hormones.