@Article{Micheletto_etal2016,
author="Micheletto, Y. M. S.
and Marques, C. M.
and Silveira, N. P. da
and Schroder, A. P.",
title="Electroformation of Giant Unilamellar Vesicles: Investigating Vesicle Fusion versus Bulge Merging",
journal="Langmuir",
year="2016",
volume="32",
number="32",
pages="8123--8130",
abstract="Partially ordered stacks of phospholipid bilayers on a flat substrate can be obtained by the evaporation of a spread droplet of phospholipid-in-chloroform solution. When exposed to an aqueous buffer, numerous micrometric buds populate the bilayers, grow in size over minutes, and eventually detach, forming the so-called liposomes or vesicles. While observation of vesicle growth from a hydrated lipid film under an optical microscope suggests numerous events of vesicle fusion, there is little experimental evidence for discriminating between merging of connected buds, i.e., a shape transformation that does not imply bilayer fusion and real membrane fusion. Here, we use electroformation to grow giant unilamellar vesicles (GUVs) from a stack of lipids in a buffer containing either (i) nanometric liposomes or (ii) previously prepared GUVs. By combining different fluorescent labels of the lipids in the substrate and in the solution, and by performing a fluorescence analysis of the resulting GUVs, we clearly demonstrate that merging of bulges is the essential pathway for vesicle growth in electroformation.",
optnote="PMID:27409245",
optnote="exported from refbase (http://www.ics-cnrs.unistra.fr/publication/show.php?record=13990), last updated on Wed, 13 Sep 2017 23:18:52 +0200",
issn="0743-7463",
doi="10.1021/acs.langmuir.6b01679",
opturl="http://www.ncbi.nlm.nih.gov/pubmed/27409245",
language="English"
}