The original version of the Repeat Sequence Database (RSDB1) [2] was created based on centralized database systems (CDBSs). RSDB presently includes an enormous amount of data, with the amount of biological data is increasing rapidly. Distributed RSDB (DRSDB) is developed to yield better performance. This study proposed many approaches to data distribution and experimentally determine the best approach to obtain good performance of our database. Experimental results indicate that DRSDB performs well for particular types of queries.