Folic Acid ELISA Kit Background Information:
Folates play an important role in the synthesis of nucleic acids and some amino acids and gained recently increased interest because they belong to the group of antioxidative vitamins. In the last years the influence of folic acid supplementation to avoid abortion and dysraphism was a topic of research increasingly. Folic acid as the most stable representative of the group of folates is added to a broad range of food. Traditional methods are mostly microbiological ones, but also TLC and HPLC are applied. These methods are time consuming and need complex equipment. This ELISA test kit allows the detection :2.5 to 4 hrs, incl. sample preparation- of folic acid in supplemented food which is more rapid compared to traditional techniques :24-48 hrs.

Folate ELISA Test Principle:
The Diagnostic Automation Folic Acid quantitative ELISA test is based on the principle of the enzyme linked immunosorbent assay-ELISA method. A folic acid conjugate is bound on the surface of a microtiter plate. Folic acid containing samples or standards and an antibody directed against folic acid are given into the wells of the microtiter plate. Immobilized and free folic acid compete for the antibody binding sites