Customize your Interfere Library

Parameter

Customizable?

Example

A Case Study

How a functional genomics team used our CRISPRi libraries to knock down genes

Understanding our client

A functional genomics team aimed to knockdown a range of cancer-related genes from the Network of Cancer Genes database using CRISPRi. They approached our team to discuss how they could customize their library to target this panel.

Made-to-order library specifications

The Desktop Genetics staff provided a DESKGEN Interfere Library based on the team’s list of gene targets. The guide RNA library was synthesized as oligos and cloned into a custom retroviral plasmid vector containing a dCas9-KRAB cassette.

DESKGEN Interfere Library

Parameter

Approach

Library type

Pooled

Nuclease

dCas9

Effector

KRAB

Genome

A375 whole genome

Delivery method

Lentivirus

Vector system

Two-vector

Target frame

-1 to -200 from TSS

Full documentation

To support further investigation, we provided the researchers with a final design report and manifest of all guides and their specifications, including predicted off-target sites and mismatch data.