Cartoon Network advertised that the film would premiere on April 1, 2007, during Adult Swim. Though most media outlets speculated that it was an April Fools joke, the film ran in its entirety during the Adult Swim block, without sound, in a tiny box in the lower left corner of the screen.

Cartoon Network launched a guerrilla marketing campaign to promote this film in January 2007. Dozens of electronic devices with blinking lights were surreptitiously posted in various locations in major US cities. When lit, an image of a Mooninite extending its middle finger appeared. On January 31, 2007, the Boston Police received several reports of suspicious devices in several locations, including on a bridge support beam in a subway station beneath a major highway. The devices caused a major security scare, and bomb squads and emergency personnel were deployed. The two men who installed the devices around Boston were arrested and charged with several counts of placing a hoax device to incite panic, a felony. Turner Broadcasting, Cartoon Network's parent company, and Interference, Inc., the marketing company that created the campaign, reached a settlement with city and state officials to resolve any civil or criminal claims. The companies agreed to pay $1 million to the Boston police department, and $1 million to fund Homeland Security. Jim Samples, head of Cartoon Network, resigned. The two men who planted the devices were sentenced to community service.

Dr. Weird's loft includes many homages to the original series, including patches in the wall from the Rabbot's escape, Dr. Weird's guitar, a picture of Dr. Weird with his roommate Randall, and a piece of the Rainbow-Maker.

When Frylock dials the number from the Insane-O-Flex website, as the camera pans through the clouds to the Plutonian ship, slowing down the footage reveals Balloonenstein from Aqua Teen Hunger Force: Balloonenstein (2001) on the right side of the screen.