ab37382 - Chicken polyclonal IgY, is suitable for use as an isotype control with this antibody.

WB

1/1000 - 1/5000. Predicted molecular weight: 50 kDa.

Expect to see a band at 55kDa and another at about 48kDa, apparently a breakdown product of the 55kDa band.

ICC/IF

1/1000 - 1/5000.

Try this antibody at about between about 1:1,000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme linked methods.

PubMed: 25418722

Target

FunctionGFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Tissue specificityExpressed in cells lacking fibronectin.

Involvement in diseaseDefects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.

Anti-GFAP antibody images

IHC image of GFAP staining in a formalin fixed, paraffin embedded normal rat hippocampus tissue section. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6). The section was incubated with ab4674 at 1/1000 dilution for 15 mins at room temperature. A goat anti-chicken biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. The section was counterstained with haematoxylin and mounted with DPX.

IHC image of GFAP staining in a formalin fixed, paraffin embedded mouse normal brain tissue section. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6). The section was incubated with ab4674 at 1/1000 dilution for 15 mins at room temperature. A goat anti-chicken biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. The section was counterstained with haematoxylin and mounted with DPX.

Western blot - Anti-GFAP antibody (ab4674)This image is courtesy of an anonymous Abreview

ab4674 staining GFAP in mouse hippocampus tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with 4% PFA and blocking with 10% serum for 30 minutes at 250C was performed. The sample was incubated with primary antibody (1/500) for 16 hours at 250C in 10% NGS in PBS + 0.1% TX100. An Alexa Fluor®488-conjugated Goat polyclonal to chicken IgG was used as secondary antibody at 1/400 dilution. Staining was intensified with 2-3 minutes of retrieval with trypsin at room temperature.

ab4674 staining GFAP in rat primary astrocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.05% Triton X-100 and blocked with 5% serum for 20 minutes at 20°C. Samples were incubated with primary antibody (1/2000) for 24 hours at 4°C. ab6873, a goat anti-chicken IgY FITC (1/1000) was used as the secondary antibody.