ImageXpress Micro 4 High-Content Imaging System

ImageXpress Micro 4 High-Content Imaging System

The ImageXpress® Micro 4 High-Content Imaging System represents the culmination of four generations of imaging expertise. The latest agile design allows you to boost your research with this faster-than-ever system, while providing the option to upgrade to confocal in the future to align with your research needs.

Investigate every aspect of a cellular pathway

Combined with MetaXpress High-Content Image Acquisition and Analysis Software, the ImageXpress Micro 4 system is an end-to-end solution that enables you to interpret your images, understand your data, and explore new ideas.

Automatically acquire images 1-100x objective magnification in slides and multi-well plates up to 1536-well format. Acquire >245,000 wells per day at 4x magnification with the system’s unique multi-well crop feature. A 4x objective captures 11 mm2 of a 384-well plate to be imaged in one field-of-view while increasing magnifications allows the resolution of fine detail for analysis of subcellular events.

Images of control cells showing DNA damage detected by H2AX phosphorylation. The positive control (bottom) clearly shows a higher percentage of cells with DNA damage (green) compared to total cells (red) in the well.

Measure stem cell growth and differentiation in layers or colonies with the system's widefield focusing capabilities, brightfield imaging option, and environmental control chamber for extended period of time

Observe rapid kinetic cellular responses over a span of several days using fluorescent or unlabeled cells kept in an environmental control chamber, together with a brightfield microscope, and on-board fluid addition capabilities

10 position excitation filter wheel increases the number of preconfigured filters installed with systems to support multi-pass dichroic and emission filters in core facilities with a large variety of fluorophores in use.

Wheel is inserted into the light source so additional space is not required

Near-IR optimized solid state whitelight engine with long lifetime, suitable for most fluorescent applications enhanced for near-IR application like Cy7

Lifetime: 10,000+ hrs. Wavelength: 380-770 nm

Optional upgrade for ImageXpress Micro 4 system

Configurable solid state light engine is a customizable excitation source with independent control of up to 7 wavelengths. It expands flexibility of wavelength selection and is compatible with multi-pass excitation cubes for additional throughput and speed.

The ImageXpress Micro 4 High-Content Imaging System enables contrast imaging of unstained samples to monitor cells that may be overly sensitive to the effects of fluorescent labels or need to be monitored over time without intervention. The Phase Contrast option can be used to scrutinize cell quality before and after biochemical assays, or combined with fluorescent applications and environmental control to examine multiple parameters at once.

Features

Phase optics enable high contrast, label-free cellular imaging to be performed at a variety of magnifications (4x -60x)

The Environmental Control Option for the ImageXpress Micro 4 High-Content Imaging System can be configured to mimic conditions within a laboratory incubator (temperature control, gas mixture and humidity) to keep cells alive for long periods of time, making high-content screening kinetic and time lapse experiments a reality.

Features

User-defined premixed air allows researchers to select atmospheric conditions most appropriate for their cell type (e.g. 5% or 10% CO2).

Temperature control of the sample plate helps maintain physiological environment.

Integrated pipettor (3-200 µL) facilitates media replenishment or compound addition to individual wells within a microplate. Media replenishment can extend the time frame of long time lapse experiments and overcome any evaporation that may occur over time

Though the technical specifications of the ImageXpress Micro 4 system are different than that of our legacy ImageXpress system products, the widefield mode covers the same applications as the legacy widefield only products.