A testosterone test measures the amount of the male hormone, testosterone, in the blood.

Alternative Names:

Serum testosterone

How the test is performed:

Blood is typically drawn from a vein, usually from the inside of the elbow or the back of the hand. The site is cleaned with germ-killing medicine (antiseptic). The health care provider wraps an elastic band around the upper arm to apply pressure to the area and make the vein swell with blood.

Next, the health care provider gently inserts a needle into the vein. The blood collects into an airtight vial or tube attached to the needle. The elastic band is removed from your arm. Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding.

In infants or young children, a sharp tool called a lancet may be used to puncture the skin and make it bleed. The blood collects into a small glass tube called a pipette, or onto a slide or test strip. A bandage may be placed over the area if there is any bleeding.

How to prepare for the test:

The health care provider may advise you to stop taking drugs that may affect the test.

How the test will feel:

When the needle is inserted to draw blood, some people feel moderate pain, while others feel only a prick or stinging sensation. Afterward, there may be some throbbing.

Why the test is performed:

This test may be done if you have symptoms of abnormal male hormone (androgen) production.

In males, the testes produce most of the circulating testosterone. The hormone LH from the pituitary gland stimulates the Leydig cells in the testicles to produce testosterone.

In females, the ovaries produce most of the testosterone. The adrenal cortex produces another male hormone, called DHEAS.