Ancient DNA

Cross-linked DNA extracted from the 4,000-year-old liver of the ancient Egyptian priest Nekht-Ankh.

Ancient DNA (aDNA) is DNA isolated from ancient specimens.[1][2] Due to degradation processes (including cross-linking, deamination and fragmentation) ancient DNA is of lower quality in comparison with modern genetic material.[3] Even under the best preservation conditions, there is an upper boundary of 0.4-1.5 million years for a sample at around to contain sufficient DNA for contemporary sequencing technologies.[4] Genetic material has been recovered from archaeological and historical skeletal material, mummified tissues, archival collections of non-frozen medical specimens, preserved plant remains, ice and permafrost cores as well as marine and lake sediments.

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The first study of what would come to be called aDNA was conducted in 1984, when Russ Higuchi and colleagues at the University of California, Berkeley reported that traces of DNA from a museum specimen of the Quagga not only remained in the specimen over 150 years after the death of the individual, but could be extracted and sequenced.[5] Over the next two years, through investigations into natural and artificially mummified specimens, Svante Pääbo confirmed that this phenomenon was not limited to relatively recent museum specimens but could apparently be replicated in a range of mummified human samples that dated as far back as several thousand years.[6]

The laborious processes that were required at that time to sequence such DNA (through bacterial cloning) were an effective brake on the development of the field of ancient DNA (aDNA). However, with the development of the Polymerase Chain Reaction (PCR) in the late 1980s, the field began to progress rapidly.[7][8] Double primer PCR amplification of aDNA (jumping-PCR) can produce highly skewed and non-authentic sequence artifacts. Multiple primer, nested PCR strategy was used to overcome those shortcomings.

The post-PCR era heralded a wave of publications as numerous research groups tried their hands at aDNA. Soon a series of incredible findings had been published, claiming authentic DNA could be extracted from specimens that were millions of years old, into the realms of what Lindahl (1993b) has labelled Antediluvian DNA.[9] The majority of such claims were based on the retrieval of DNA from organisms preserved in amber. Insects such as stingless bees,[10] termites,[11] and wood gnats,[12] as well as plant[13] and bacterial[14] sequences were extracted from Dominican amber dating to the Oligocene epoch. Still older sources of Lebanese amber-encased weevils, dating to within the Cretaceous epoch, reportedly also yielded authentic DNA.[15] DNA retrieval was not limited to amber.

Several sediment-preserved plant remains dating to the Miocene were successfully investigated.[16] Then, in 1994 and to international acclaim, Woodward et al. reported the most exciting results to date[17] — mitochondrial cytochrome b sequences that had apparently been extracted from dinosaur bones dating to more than 80 million years ago. When in 1995 two further studies reported dinosaur DNA sequences extracted from a Cretaceous egg,[18] it seemed that the field would revolutionize knowledge of the Earth's evolutionary past. Even these extraordinary ages were topped by the claimed retrieval of 250-million-year-old halobacterial sequences from halite.[19][20]

Due to degradation processes (including cross-linking, deamination and fragmentation) ancient DNA is of lower quality in comparison with modern genetic material.[3] The damage characteristics and ability of aDNA to survive through time restricts possible analyses and places an upper limit on the age of successful samples Allentoft et al. (2012). There is a theoretical correlation between time and DNA degradation,[23] although differences in environmental conditions complicates things. Samples subjected to different conditions are unlikely to predictably align to a uniform age-degradation relationship.[24] The environmental effects may even matter after excavation, as DNA decay rates may increase,[25] particularly under fluctuating storage conditions.[26] Even under the best preservation conditions, there is an upper boundary of 0.4-1.5 million years for a sample at around to contain sufficient DNA for contemporary sequencing technologies.[4]

Research into the decay of mitochondrial and nuclear DNA in Moa bones has modelled mitochondrial DNA degradation to an average length of 1 base pair after 6,830,000 years at −5 °C.[27] The decay kinetics have been measured by accelerated aging experiments further displaying the strong influence of storage temperature and humidity on DNA decay.[28] Nuclear DNA degrades at least twice as fast as mtDNA. As such, early studies that reported recovery of much older DNA, for example from Cretaceousdinosaur remains, may have stemmed from contamination of the sample.

A critical review of ancient DNA literature through the development of the field highlights that few studies after about 2002 have succeeded in amplifying DNA from remains older than several hundred thousand years.[29] A greater appreciation for the risks of environmental contamination and studies on the chemical stability of DNA have resulted in concerns being raised over previously reported results. The dinosaur DNA was later revealed to be human Y-chromosome,[30] while the DNA reported from encapsulated halobacteria has been criticized based on its similarity to modern bacteria, which hints at contamination.[31] A 2007 study also suggests that these bacterial DNA samples may not have survived from ancient times, but may instead be the product of long-term, low-level metabolic activity.[32]

aDNA may contain a large number of postmortem mutations, increasing with time. Some regions of polynucleotite are more susceptible to this degradation, so sequence data can bypass statistical filters used to check the validity of data.[33] Due to sequencing errors, great caution should be applied to interpretation of population size.[34] Substitutions resulting from deamination cytosine residues are vastly over-represented in the ancient DNA sequences. Miscoding of C to T and G to A accounts for the majority of errors.[35]

Another problem with ancient DNA samples is contamination by modern human DNA and by microbial DNA (most of which is also ancient).[36][37] New methods have emerged in recent years to prevent possible contamination of aDNA samples, including conducting extractions under extreme sterile conditions, using special adapters to identify endogenous molecules of the sample (over ones that may have been introduced during analysis), and applying bioinformatics to resulting sequences based on known reads in order approximate rates of contamination.[38]

In June 2013, a group of researchers announced that they had sequenced the DNA of a 560–780 thousand year old horse, using material extracted from a leg bone found buried in permafrost in Canada's Yukon territory.[45]

Researchers in 2016 measured chloroplast DNA in marine sediment cores, and found diatom DNA dating back to 1.4 million years. [47] This DNA had a half-life significantly longer than previous research, of up to 15,000 years. Kirkpatrick's team also found that DNA only decayed along a half-life rate until about 100 thousand years, at which point it followed a slower, power-law decay rate. [47]

Due to the morphological preservation in mummies, many studies from the 1990s and 2000s used mummified tissue as a source of ancient human DNA. Examples include both naturally preserved specimens, for example, those preserved in ice, such as the Ötzi the Iceman (Handt et al. 1994), or through rapid desiccation, such as high-altitude mummies from the Andes (c.f. Pääbo 1986; Montiel et al. 2001), as well as various sources of artificially preserved tissue (such as the chemically treated mummies of ancient Egypt).[48] However, mummified remains are a limited resource. The majority of human aDNA studies have focused on extracting DNA from two sources that are much more common in the archaeological record – bone and teeth. Several other sources have also yielded DNA, including paleofaeces (Poinaret al. 2001) and hair (Baker et al. 2001, Gilbert et al. 2004). Contamination remains a major problem when working on ancient human material.

Ancient pathogen DNA has been successfully retrieved from samples dating to more than 5,000 years old in humans and as long as 17,000 years ago in other species. In addition to the usual sources of mummified tissue, bones and teeth, such studies have also examined a range of other tissue samples, including calcified pleura (Donoghue et al. 1998), tissue embedded in paraffin,[49][50] and formalin-fixed tissue.[51] Efficient computational tools have been developed for pathogen and microorganism aDNA analyses in a small (QIIME) and large scale (FALCON[52]).

Taking preventative measures in their procedure against such contamination though, a 2012 study analyzed bone samples of a Neanderthal group in the El Sidrón cave, finding new insights on potential kinship and genetic diversity from the aDNA.[53] In November 2015, scientists reported finding a 110,000-year-old tooth containing DNA from the Denisovan hominin, an extinctspecies of human in the genus Homo.[54][55]

The research has added new complexity to the peopling of Eurasia. It has also revealed new information about links between the ancestors of Central Asians and the indigenous peoples of the Americas. In Africa, older DNA degrades quickly due to the warmer tropical climate, although, in September 2017, ancient DNA samples, as old as 8,100 years old, have been reported.[56]

^Pruvost, M. et al., 2007. Freshly excavated fossil bones are best for amplification of ancient DNA. Proceedings of the National Academy of Sciences of the United States of America, 104(3), pp.739–744.