B-70

Ubiquitin Conjugation Buffer is formulated to support the charging of Ubiquitin by E1 activating enzymes (the first reaction in the Ubiquitin conjugation cascade). This buffer is similar in composition to 10X E3 Ligase Reaction Buffer (B-71), but contains a lower concentration of reductant for those enzymes that may be sensitive to elevated disulfide-reducing potential. Fully compatible with E1 and E2 enzyme activity, this buffer can be used to supplement the Ubiquitin-protein conjugation kit (K-960), Fraction II conjugation kits (K-930, K-935), and E3-ligase enzymes. Also compatible with deubiquitinating enzymes (DUBs).