I am doing nitrogen evaporation of a sample of proteins eluted from a sep-pak carteridge in ethanol/6 N hydrochloric acid (1,000:1, v/v). The protein of interest is Oxytocin.

My question is: should I use heat the sample while evaporating? I would think that heating would cause more rapid evaporation, but cold temperature would be safer for protein integrity.. However, will the EtOH/HCL solvent evaporate efficiently without heat??