PCR Reagent

EzWay Plant Direct PCR Kit

K0568700

EzWay™ Plant Direct PCR Kit enables DNA amplification directly from plant leaves without DNA extraction. Punch to isolate disks of leaf tissue and incubate it in the lysis buffer provided in the kit for 10 minutes and then use 1ul of lysate for PCR. Or just leaf itself (when using 0.5 mm punched leaf disc) can be used as a template directly without lysis step. Direct Taq PCR MasterMix in this kit is a convenient mixture of Taq enzyme, dNTP, MgCl2, dye, additives and Direct PCR Buffer, so you can just add the leaf extract and primers, and then go for PCR. This kit will save your enormous amounts of material, cost and time from conventional PCR methods using mechanical disruption, organic extraction, column DNA purification, or alcohol precipitation. Also, it reduces risk of handling errors and contamination.