Amyloidogenic Pathway Protein Detection

The accumulation of amyloid-beta protein in the cerebral cortex in Alzheimer's disease (AD) is one of the outcomes of the aberrant proteolytic cleavage of amyloid precursor protein (APP). Aberrant cleavage can occur due to the more than 50 mutations documented in the APP gene that are associated with early-onset Alzheimer's disease.

In the amyloidogenic pathway, cleavage of APP by beta-site APP cleaving enzyme 1 (BACE1), also known as beta-secretase, results in a beta-C-terminal fragment (beta-CTF). This fragment is further cleaved by gamma-secretase to produce insoluble amyloid beta and soluble APP-beta proteins. Amyloid-beta proteins that build up in brain parenchyma are mostly produced from APP expressed in neurons, APP695. A different form of APP, APP770, has been detected in cerebrovascular endothelial cells. Amyloid beta derived from APP770 can accumulate on cerebrovascular walls.

BACE1 is a transmembrane protein that contains two aspartate residues in its active site. Elevated levels of BACE1 have been correlated with late-onset sporadic AD.

ELISAs for APP beta-CTF and APP770 detection

These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to human APP770 or APP beta-CTF protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This kit can be used to measure human APP770 or APP beta-CTF protein in EDTA plasma, cerebrospinal fluid, cell culture cupernatant, or cell lysates.

The Human BACE1 Antibody products are affinity-purified IgG antibodies that recognize human BACE1 protein. The BACE1 antibodies are raised in rabbit using synthetic peptides and can be applied to Western blot (WB) and immunohistochemistry (IHC) detection as well as immunoprecipitation (IP) of human BACE1 protein.

Features

Does not cross‑react with human sAPP-alpha, sAPP-beta, or sAPP-beta-sw

APP770

27736A

ELISA

Human

Capture:(351)Rabbit IgG

Detect: HRP (R101A4)Mouse IgG MoAb

Does not cross-react with human APP695 or APP751

APP770

28133A, B

WBIHC

Human

(351)Rabbit IgG

n/a

APP(N-terminus)

11090A, B

WBIP

Human

(10D1)Mouse IgG MoAb

Does not cross-react with mouse APP

Reacts with all isoforms of APP695, APP751, and APP770, and soluble APP (sAPP-alpha and -beta)

APP(C-terminus)

18961A, B

WBIP

Human

(n/a)Rabbit IgG

Cross-reacts with mouse and rat APP

Reacts with all isoforms: APP695, APP751, and APP770

Does not react with soluble APP (sAPP-alpha or -beta)

APP (phosphorylated)

28021A, B

WBIP

Human

(n/a)Rabbit IgG

Cross-reacts with mouse phosphorylated APP

Does not cross-react with non-phosphorylated APP

APP

28053A, B

WB

Human

(18)Rabbit IgG

Cross-reacts with mouse and rat APP

Cross-reacts with sAPP-alpha and sAPP-beta

sAPP-alpha(C-terminus)

28055A, B

WBIP

Mouse Rat

(597)Rabbit IgG

Does not cross-react with human sAPP-alpha, or human/mouse/rat sAPP-beta

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.