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ANTI-INFLAMMATORY ACTIVITY OF P. OSTREATUS (JACQ.:FR.) KUMM. EXTRACTS Inesa A. Avagyan 1, 2, Lia A.Minasbekyan 1, Arus G. Zhamgaryan 2, Hakob V.Topchyan 2 1. Faculty of Biology, Yerevan State University, str. A. Manougian, 1, Yerevan, 0025, Armenia, E-mail: inavag@rocketmail.com 2. Faculty of Farmacology, Yerevan State Medical University, str. Heratsu, 2, Yerevan 0025, Armenia Basidial macromycetes are not only value food, but can use as source of biological active compounds as the genistein,  -glucans, glioxal–oxidase et al. Early we investigated the influence of the non-thermal extremely high frequency electromagnetic waves in the interval of 45-53GHz on mycelial culture’s growth, β-glucosidase and peroxidase activity of wood-decaying mushroom. The aim of this investigation is obtained extracts from mushroom culture with increased peroxidase activity and study its anti-inflammatory property. Anti-inflammatory activity of mushroom’s extract investigate on the model of ear acute inflammation stimulated by xylol on white underbred rat-male with weight approximately 150 – 180g. Anti-inflammatory activity estimated by mass difference of inflammatory and control ears after 90 min of fresh extract administration from mushroom’s culture, treated by different frequency of mm-waves. As evidence our investigations intraperitoneal introduction of extracts in a dose 2,5 ml/kg led to the decreasing of induced by xylol ear swelling compare with control up to 85%. Obtained data evidence about high anti-inflammatory activity of extracts from culture of wood-decaying mushroom’s, due to increased fermentative systems, having antioxidant activity and be able to inhibited ferments, participate in syntesis and metabolism of prostoglandins.inavag@rocketmail.com INTRODUCTION Recently, much importance search of drugs containing a set of active compounds that can affect on the various links in the development of chronic inflammatory diseases. This is due to an increased interest to the natural sources of medicinal substances, which having a rich composition of biologically active compounds are a potential and relatively safe source for a new anti-inflammatory drugs. Medicinal plant content biologically active compounds, among which special attention should be phenol compounds, saponins, terpenoids and fatty acids. Antiviral drugs based on fungi consist in the ability to block the viral enzyme, synthesis or adsorption of viral nucleic acid, and the introduction of viruses in the mother cells, as well as indirectly - in the immunostimulatory activity of polysaccharides or other complex molecules. AIM OF RESEARCH Phenol compounds exhibit strong antibacterial, antiviral and antioxidant activity, and possess neuroprotective and anti-tumor effect. The structure of secondary cell wall of wood-rot mushroom - Pleurotus ostreatus consist from chitin, which is contain glucans and lignin (a polymer of phenylpropanoids). The mushroom is also rich by β-glucosidase and peroxidases, necessary for synthesis and cleavage of lignin and polysaccharides and their derivatives. Previously we have shown that at certain frequencies of EHF EMR some of the enzymes of mushroom rapidly activated. Therefore, we have assumed that the extract from fresh culture of the mushroom Pleurotus ostreatus will be effective in use as anti-inflammatory drug. The aim of this study was to identify the effectiveness of the use of these extracts from pure cultures of Pleurotus ostreatus with enzymatic activity modulated by mm-waves. As a model of antiinflammatory activity were rats ears acute. APPLICATION SCENARIO Study on determination of anti-inflammatory activity of the extracts of mushroom was carried out on models of rat ear acute inflammation induced by xylol. The weight of inflamed ear, induced by xylol exceeds of no acute ear from the same rat, as even visually seen in the pictures. Two ears cut at a rat are presented: one without an inflammation as control, and another inflamed, induced by a xylol. On a difference of weights of these ears also judge influence of an extract on inflammatory process. Rat-male with weight 150 – 180g Pleurotus ostreatus Xylol control 45-53 GHz Intraperitoneal introduction of an extract of the mushroom prepared on the basis of the Tris- buffer in a dose of 5 mg/kg for 30 mines before cutting off of tips of both ears, is accompanied by reduction of the acute induced by a xylol that is shown in reduction of a difference between rat ears weight in different degree in comparison with a control extract from not irradiated culture of a mushroom. Distinctions in influence of an extract depending on frequency of EHF EMR of preliminary processing cultures are observed. If a difference of weights of ears at influence of a control extract to accept for 100 % the extract from the culture of a mushroom preliminary irradiated of 50.3 GHz during 40 mines, reduces exudative edema of an ear approximately by 40 % of percent (Pic.1). It, apparently, is connected with high anti-oxidant activity of mushroom extracts. As it is presented on picture difference of weight of the inflamed and not inflamed ear in control rats by whom the extract preliminary has not been entered intraperitoneal, have accepted for 100 % (Pic.2). Then the inflammation of an ear induced by a xylol at a rat by whom it has preliminary been intraperitoneal injection of an extracts both from not irradiated and the irradiated mm- waves culture of a mushroom on frequency 50.3GHz during 40 min reduces an inflammatory acute by 85 % (Pic.3). Such effective influence of this extract may be explain by increase of peroxidase activity in culture of a mushroom on 3 day after an irradiation 50.3GHz during 40 min (Pic.4). Extracts from a mushroom irradiated 49GHz during 20 and 40 min, do not lead to inflammation reduction, predictably judging by PO activity in mushroom’s cultures of a though at these frequencies reaches the maximum activity  -glucosidase (Pic.5). On the base of these data it is possible to assume that for anti-inflammatory activity responsible first of all a peroxidase splitting a lignin (Pic.6) to the phenol derivatives, instead of  -glucosidase. After the preliminary introduction of the extract indicated the presence of weakly expressed edema. In the inflamed tissue revealed diffuse cellular infiltrates, which are mainly very scattered, in the case when the control sections in the main focus inflammation infiltrates. The number of cellular infiltrates is also less compared with the control (1:100) (Right). In nearby veins tissue of the dermis there is accumulation lymphomacrofages cellular elements, and there is congestion of vessels. (1:400) (Left control). This result again evidence about effectiveness of obtained by us extracts. CONCLUSION The data indicate that the extract of the wood-rot mushroom has a pronounced anti- inflammatory activity, which is likely due to the presence of identified in the extract of the enzyme systems which, according to obtained data showing strong antioxidant activity and may inhibit the activity of enzymes involved in synthesis and biotransformation of prostaglandins. REFERENCES Avagyan, I.A., Nanagulyan S.G., Balasanyan M.G., Zhamgaryan A.G. Antiinfalammatory activity of extracts of Pleurotus ostreatus mushroom// Immunopathology, Allergology, Infectology, 2010, №1, P.236. Minasbekyan L.A., Nanagulyan S.G., Avagyan I.A. Increasing of  -glucosidase activity of Pleurotus ostreatus cultures on replay of stress influence.// Immunopathology, Allergology, Infectology, 2009, №1, P.26-27. Nanagulyan S.G., Nerkararyan A.V., Avagyan I.A., Minasbekyan L.A.Минасбекян Increasing of peroxidase activity of Pleurotus ostreatus (Jacq.:Fr.)Kumm. cultures on replay of stress influence.// II Symposium Micologist of Russia, Moscow, 2008, P. 135- 136. Pic.1 Pic.2Pic.3 Pic.5Pic.4 Pic.6

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