All But Confirmed: The Penguin, Poison, Freeze and Scarecrow were allegedly seen in the concept art stages, in game clues or hints given by Eidos to a Game Informer reporter. Amygdala has reportedly been seen in the trailers.

The PenguinMr. FreezeThe ScarecrowPoison IvyAmygdala

Possible

Two-Face coins are allegedly in the game, believing he may be.Two-Face

Bruce Wayne / Batman. He will be played by voice actor Kevin Conroy, who reprises his role as Batman from DC animated universe. The Joker: He will be an inmate in the asylum and the main antagonist. He will be played by voice actor Mark Hamill, who reprises his role as the Joker from the DC animated universe and Birds of Prey. Commissioner James Gordon. (Voiced by Tom Kane) Barbara Gordon / Oracle: A radio contact for Batman throughout the game. (Played by: Brittany Byrnes) Waylon Jones / Killer Croc: Screenshots show him as an enormous, reptilian creature, towering over Batman. (Played by: Clancy Brown). Edward Nygma / The Riddler (Played by: James Arnold Taylor). Harley Quinn (Played by Arleen Sorkin, reprising her role as Harley Quinn from the DC animated universe)The Scarecrow (voiced by: David Kaye) Mr. Freeze (played by: Kirk Thornton)Bane is also confirmed for the game. (played by Richard McGonagle) Mr. Zsasz (played by: Danny Jacobs) will also be a villain in the game.The Penguin (played by: David Sobolov)Poison Ivy (played by: Michelle Ruff)