Experimental Research; Hepatic cooling in situ, partial liver transplantation, and autotransplantation of partial liver have been successfully performed using biegle dogs. The survived dogs showed early recovery of arterial ketone body ratio after reperfusion.Clinical applications; 1. The cirrhotics tolerate one hour in total vascular exclusion under the veno-venous bypass, by which hemihepatectomy, combined resection and reconstruction of IVC are performed. 2. Cooling in situ technique is applied to prevent the postoperative liver insufficiency, when it is estimated that the vascular exclusion would exceed one hour. 3. The local excision of a malignant tumor was performed on the back table after left hemihepatectomy of malignant bearing lobe, and then the remnant tumor free part was transplanted in a cirrhotic case, since the volume of the right lobe was estimated not enough to response to the stress from whole body after left lobectomy.