Neuronal Tracing

Neuronal morphology and connectivity are key components in neuronal function. Molecular Probes® labeling and detection tools include a diverse selection of probes for dissecting neural networks and their functions, with an extensive palette of fluorescent colors for each class of probes.

Fluorescent tracers have the advantage of being imaged directly, or they can serve as haptens for antibodies or streptavidin dye conjugates used to amplify the signal.

The technologies used to trace neural networks can be divided into those for tracing single cells and those for monitoring cell populations. While most fluorescent probes are effective in experiments involving fresh tissue sections and cultured cells, there are special requirements for fixed tissues and in vivo imaging.

Hydrazides & Biocytins

Loaded by microinjection or iotophoresis, these fixable and photostable probes are typically employed to trace neuronal projections and investigate gap junctions.

Dextran Conjugates

Protein Conjugates

Useful for live or fixed cells and introduced by microinjection or iontophoresis, this range of fixable, site-selective fluorescent probes provides choices for axonal membrane staining and neuronal sybtype differentiation.