Description:
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases suc h as cancer (reviewed in 1). Aven is a recently discovered protein that blocks apoptosis induced by Apaf-1 and caspase-9 (2). It is thought that Aven functions by binding to Bcl-xL, an antiapoptotic member of the Bcl-2 family, and to Apaf-1, possibly interfering with the ability of Apaf-1 to self-associate, suggesting that Aven impedes Apaf-1-mediated caspase activation. Higher levels of Aven mRNA are seen in patients with acute leukemia than in control patients, suggesting that Aven may be useful as a prognostic indicator in leukemia patients (3). Despite its predicted molecular weight, Aven often migrates at 55kD in SDS-PAGE.

Applications:Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:Western Blot: 0.5-1ug/ml. See A4445-11A for control peptide.Optimal dilutions to be determined by the researcher.

Positive Control:Raji cell lysate

Storage and Stability:May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.