The MicroRotofor cell uses liquid-phase IEF to fractionate small volumes (2.5 ml) of proteins in free solution by their pI. The MicroRotofor cell can be used to reduce the complexity of samples prior to any protein analysis technique (including mass spectrometry and 2-D electrophoresis). In 2-D electrophoresis applications, protein fractionation by pI improves downstream sample loading and separation on narrow- and micro-range IPG strips. Whether used alone or as part of a broader separation or purification scheme, the MicroRotofor cell offers rapid, simple, and effective fractionation and enrichment of proteins from complex protein mixtures.

Integrated temperature regulation to improve reproducibility and to preserve protein structure and function for native separations

Preassembled disposable focusing chambers

Accessory equipment required to run the MicroRotofor cell includes a 1,000 V power supply such as the PowerPac™ HV power supply (which is capable of running at 1 W constant power) and house vacuum. Bio-Lyte ampholytes are required to form the internal pH gradient.

Related Products

Different configurations of the Rotofor system are available to accommodate different sample loads. The Rotofor®, Mini Rotofor, and MicroRotofor cells all share the following features:

Focusing and enrichment of a protein of interest into 1 or 2 fractions in a single step

Rotofor Cell

Mini Rotofor Cell

MicroRotofor Cell

Number of fractions

20

20

10

Sample volume

35–60 ml

18 ml

2.5 ml

Fraction volume

1.75–3 ml

0.7 ml

200–250 µl

Sample load

Milligram to gram

Microgram to milligram

Up to to 1 mg

MicroRotofor cell lysis kits provide convenient cell lysis and protein extraction protocols to generate total protein samples that are ready for fractionation with a MicroRotofor IEF cell or other downstream applications such as SDS-PAGE and 2-D gel electrophoresis. Kits are available for mammalian, bacterial, plant, and yeast cells.

What is the education discount policy?

For more than 15 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows high school and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices.

How do I apply for the education discount?

If you are an educator at the high school or college level, visit our Education Discount Policy page to establish an education account number.