Introduction: The DNA sequencing is the most important technique in molecular biology by which the order of the nucleotides can be identified in a piece of DNA. There are several different methods for sequencing the DNA. Now, the DNA sequencing has great importance in the medical diagnostics and other medical fields. Some methods have been invented to speed up and increase the efficiency of the DNA sequencing by advances in the fields of nanotechnology and bioinformatics. This made it possible to perform larger, cheaper, faster, and more accurate projects, such as the sequencing of the human genome.Methods and Materials: This review article is a content analysis study that has been performed by searching DNA sequencing and DNA sequencing techniques with high throughput key words in google scholar, Science Direct, PubMed and Scopus websites.Results: Due to the importance of the DNA sequencing and its applications in the various fields, scientists intend to present new methods to decrease the expense and time with the accuracy increment and high throughput.Discussion and Conclusion: Along with increasing tendency of Iranian researchers in this area, this paper tried to provide the larger insight in conjunction with these methods amongst the researchers by introducing the different techniques of genome sequencing.