Royal jelly has a high nutritional and pharmaceutical value and its demand is continually increasing in today’s world market. The determination of the geographical origin of royal jelly is very important both for the producers and the consumers. At present, melissopalynological analysis is the main tool for the determination of the geographical origin of royal jelly. In this study, we microscopically examined royal jelly produced by honey bee colonies fed with different pollen supplements. The results showed that the pollen grains from the supplemental foods can be found in significant quantities in royal jelly, and their abundance might vary according to the size of the pollen grain. Therefore, the use of pollen supplements during royal jelly production can affect the accuracy of the melissopalynological results.

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