Heme Oxygenase-1 Detection

Heme oxygenase-1 (HO-1) is a microsomal enzyme that degrades heme, a prosthetic group of the heme protein family (e.g., hemoglobin), into the bile pigment biliverdin. Biliverdin is subsequently converted to bilirubin, carbon monoxide, and reduced iron. Bilirubin has an anti-inflammatory effect and is part of the oxidative stress response due to its strong free radical scavenging activity, whereas carbon monoxide has a vasodilatory effect on organ blood flow.

Heme oxygenase exists in at least two isozyme forms: heme oxygenase-1 and heme oxygenase-2, each of which differs in tissue expression and inducibility. Heme oxygenase-1 in particular is intracellularly induced as part of the oxidative stress response; therefore, heme oxygenase-1 monitoring may prove useful for the identification of physiological stress inducers.

ELISAs for Heme Oxygenase-1 Detection

The Rat Heme Oxygenase-1 EIA Kit (Precoated) is a solid phase sandwich ELISA that utilizes two mouse monoclonal heme oxygenase-1 antibodies, one of which is coated on the plate and the other of which is peroxidase-labeled. This 96-well format in vitro enzyme immunoassay kit is intended for the quantitation of rat heme oxygenase-1 in blood, cell culture supernatant, and various organ systems.

Antibodies for Heme Oxygenase-1 Detection

Anti-Heme Oxygenase-1 Antibody, Monoclonal (Clone GTS-1) and Anti-Heme Oxygenase-1 Antibody, Monoclonal (Clone GTS-3) are sodium azide-free and can be used directly form heme oxygenase-1 inhibition assays. These antibodies can also be used for detection of heme oxygenase-1 by Western blot (WB) and immunohistochemistry (IHC) in frozen and paraffin-embedded tissues.

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.