Target

FunctionCore subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

ab110246, at 10 µg/ml, staining NDUFS3 in cultured Human fibroblasts by Immunofluorescence. The cells were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110246, followed by a fluorescent goat-anti-mouse IgG.

ab110246 was labeled with a universal probe (which recognizes antibodies raised in both mouse and rabbit species) for 30 minutes and a HRP-polymer probe for 30 minutes. ab110246 was diluted and incubated for 1 hour with the target. Antigen retrieval was not required.

ab110246 was labeled with a universal probe (which recognizes antibodies raised in both mouse and rabbit species) for 30 minutes and a HRP-polymer probe for 30 minutes. ab110246 was diluted and incubated with the target for 1 hour. Antigen retrieval performed by 1 min pressure cooking with 1mmol EDTA, pH8.

Thank you for your reply. For a use on tissue sections I can recommend to use an antibody directed against NDUFS3 (or Complex I 30kDa subunit) such as ab110246. The link to the datasheet of this antibody is www.abcam.com/ab110246. I hope this...

Thank you very much for contacting us with your question. The lab does use an antigen retrieval step while staining cultured cells with ab110246, so I would recommend using this step. Our ICC protocol with antigen retrieval can be found at this li...

We prepared this antibody by immunization of the entire complex NADH dehydrogenase.The reactivity with NDUFS3 was proved by reaction with recombinant human NDUFS3 expressed in E.coli.Unfortunately we don’t know t...