Summary: The Snf1 kinase and proteasome-associated Rad23
regulate UV-responsive gene expression
Staton L Wade, Kunal Poorey, Stefan
Bekiranov and David T Auble*
Department of Biochemistry and Molecular Genetics, University of
Virginia Health System, Charlottesville, VA, USA
The transcriptional response to damaging agents is of fun-
damental significance for understanding mechanisms re-
sponsible for cell survival and genome maintenance.
However, how damage signals are transmitted to the tran-
scriptional apparatus is poorly understood. Here we identify
two new regulators of the UV response transcriptome: Snf1,
a nutrient-sensing kinase, and Rad23, a nucleotide excision
repair factor with no previously known function in tran-
scriptional control. Over half of all UV-responsive genes are
dependent on Snf1 or Rad23 for proper regulation. After
irradiation, Snf1 targets the Mig3 repressor, a new effector of
the UV response. Snf1 and Rad23 are both required for the
displacement of Mig3 from the UV-activated HUG1 promoter,
and Rad23's activity is functionally linked to the proteasome