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Faster, Easier and More Reliable

than Liquid-Liquid Extraction.

Novum SLE Information

Simplify Your Liquid-Liquid Extractions

Novum Simplified Liquid Extraction (SLE) (patent pending) products are a FASTER, EASIER, and MORE RELIABLE way to perform liquid-liquid extractions. In 3 easy steps and less than 15 minutes, you can remove unwanted interferences such as salts, proteins, phospholipids and more from a variety of samples without performing extensive method development. Available in several capacities, Novum SLE is an excellent cleanup solution for a wide range of samples including biological, food, personal care products, and more.

As the first synthetic SLE product on the market, Novum provides consistent, reliable results from lot-to-lot which is sometimes difficult to do when using a natural product such as traditional diatomaceous earth SLE.

See What Makes Novum Different

Novum FAQS

If you are already performing a liquid-liquid extraction, you may use the same buffers and solvents you are using in your liquid-liquid extraction to perform SLE.

Elution Solvent Selection

In most cases, using ethyl acetate as an elution solvent results in an excellent balance between cleanup and recovery.

If you are using DCM as an elution solvent and see low recovery of target analytes (particularly fluorinated analytes or those that have a negative logP value), recovery may be improved by adding 10-20 % Ethyl acetate to the DCM during the elution step.

Elution Volumes

If desired, you may apply multiple aliquots of elution solvent. For example, 3x 600 µL of elution solvent may be used instead of 2x 900 µµL on the Novum SLE MAX 96-well plate.

Boosting Analyte Recovery

The Novum SLE sorbent relies on mass transfer to help partition target analytes out of the aqueous sample and into the organic elution solvent. In order to boost recoveries of some assays, it may be necessary to dilute your sample more than the suggested 1:1 dilution in order to increase the surface area of the aqueous sample. See Recommended Loading Volumes in the General Protocol to determine the total load volume (sample plus diluent) for each Novum SLE product.

It may also be possible to boost analyte recoveries by increasing the amount of elution solvent applied to the sorbent.

Longer elution times will also improve extraction efficiencies in many cases. If vacuum must be applied, it is recommended that the vacuum pressure applied results in drop wise elution of the extraction solvent.