Fluofarma offers a panel of validated cell-based assays allowing the quantification of key phenotypic and molecular events at the single-cell level and enabling to evaluate the potential benefits of compounds. Most of the cellular assays listed below can be performed on our automated screening platforms. Assays can be adapted to a different cell model, or fully developed, according to your need.

Human skin staining

FLUOFARMA is able to handle, stain (IHC or multiplex in IF) and quantify biomarkers with spatial information at the single-cell level allowing subpopulation analyses in Human skin explant. For example :

Skin Biomarker Quantification

Processing of IHC images using color and texture information. Once the layer of interest inside the skin has been identified, we can compute several readouts such as cell count or thickness. For example :

- Loricrin staining: Loricrin area is extracted using color features to compute its thickness- Ki67 staining: Cell counting inside the epidermis and identification of the Ki67 sub-population e.g. Percentage of Ki67 positive cells inside the epidermis (brown stained cells)

Skin Layers Identification & Quantification

Color and Texture information and Supervised Machine Learning algorithms can be used to identify the different skin layers of Hematoxylin Eosin images inside a database. It is possible to quantify the morphology of skin tissue such as epidermal thickness, percentage, etc.