The flowers of Pongamia pinnata was extracted with methanol was subjected to preliminary phytochemical analysis. Flavonoids, alkaloids, carbohydrates, tannins, lignins, saponins, glycosides and fixed oil and fats were found to be present. The methanolic crude extract further fractionated with three different solvents, i.e. benzene, dethyl ether and ethyl acetate.and the chemical composition of the ethyl acetate extract was determined by GC/MS analysis.The present investigation revealed the following major compounds from P.pinnata. In this analysis 30 bioactive phytochemical constituents were identified. Based on the peak area and molecular weight, the dibutyl phthalate and oleic acid was observed as the major constituents.
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Endophytic bacteriareside with in plant hosts without causing disease. In our study, we isolates four endophytic bacteria from explants (root,stem, petiole, and leaf) of Aloe Barbadensis, Adhatoda vasica, Phyllanthus amarus and Calotrophis gigantea.Entophytic bacteria such as Azotobacter,Xanthomonas and Pseudomonas could be identified from explants through various biochemical test. Endophytic bacteria were beneficial to the plant growth and it can be proved that inoculate to Mustard plant which was showed good growth promoting effect. The endophytes contributes substances that possess biocontrol activity against Curvularia sp., and Alternaria sp.,.Thus in our study,we focus on the growth promoting effect of endophytic bacteria in mustard plant and biocontrol activity against fungal pathogens.
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