We suspect the problem come from the competent cells we used because we used a different stock for 1.A and 1.E

1.E giving bad results from the beginning it might come from a bad transformation .

We will verify this theory next week by changing our competent cells .

Next week we will also extract the plasmids needed for the construction of the third operon because we are still waiting the requested biobricks (BBa_K091106, BBa_K091001, BBa_K091002 )to be delivered to construct our second an fourth operon