Application Information: Myoglobin antibody

This antibody may be diluted to a titer of 1:50-1:200 in an ABC method. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user.

Form Supplied

Liquid

Concentration

4
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Protein A purified

Specifications: Myoglobin antibody

Full Name

myoglobin

Background

Myoglobin is a small heme-containing protein (153 amino acid residues, molecular weight (w/o heme) 17053 Da and theoretical pI=7.29) responsible for the oxygen deposition in muscle tissues. Only one form of myoglobin is expressed in cardiac and skeletal muscles. Myoglobin is known as a marker of myocardial damage and it has been used for more than three decades. Nowadays it still is very commonly used in clinical practice as an early marker of AMI. It appears in patient's blood 1-3 hours after onset of the symptoms, reaching peak level within 8-12 hours. Myoglobin is not so cardiac specific as cTnI or cTnT. Because of high myoglobin concentration in skeletal muscle tissue, even minor skeletal muscle injury results in the significant increase of myoglobin concentration in blood. Thus myoglobin is used together with cTnI or cTnT in clinical practise for better specificity in AMI diagnosis.

Synonyms

160000, MGC13548, Myoglobin, P02144, MB, PVALB, 4151

Specificity

This antibody reacts with human myoglobin. The antibody stains strongly with skeletal and cardiac muscles. No non-specific staining with other tissues has been observed.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

Myoglobin isolated from human heart.

Antigen Species

Human

Species Reactivity

Human

Conjugation

Unconjugated

Storage Conditions: Myoglobin antibody

Storage Buffer

This antibody contains sodium azide.

Storage Instruction

Keep as concentrated solution, aliquot and store at 4°C.

Notes

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.