Mastercycler® gradient by Eppendorf

The Eppendorf Mastercycler gradient sets the standard in the modern molecular biology laboratory, with the Gradient PCR being the first-choice method for optimizing reaction conditions. A freely programmable temperature gradient of up to 20°C enables the optimization not only of the annealing temperature, but also of all temperature steps of a PCR protocol for even the most demanding applications. Thanks to SteadySlope Technology, optimal, constant heating and cooling rates are guaranteed, which... Read more

The Eppendorf Mastercycler gradient sets the standard in the modern molecular biology laboratory, with the Gradient PCR being the first-choice method for optimizing reaction conditions. A freely programmable temperature gradient of up to 20°C enables the optimization not only of the annealing temperature, but also of all temperature steps of a PCR protocol for even the most demanding applications. Thanks to SteadySlope Technology, optimal, constant heating and cooling rates are guaranteed, which means that results of gradient experiments can be transferred simply and precisely to routine applications.

An additional heating and cooling zone is available as a result of Triple Circuit Technology, which ensures precise control of the temperature for all gradient profiles.

Mastercycler is the economical option when, after having optimized your PCR using Mastercycler gradient, you wish to carry out an experiment with the usual Mastercycler quality, but without blocking your optimization cycler.

The entire Mastercycler family boasts the personal card system. Chip-based memory cards enable not only simple and reliable transfer of your individual protocols between different Mastercycler workstations but also ensure unlimited expansion of the memory capacity.

The universal block for different tube types extends the applicational range of Mastercycler and Mastercycler gradient as well as saving time and money. It is possible to use 96 x 0.2 ml tubes, 77 x 0.5 ml tubes or one microtiter plate - without having to change the block.