Mutation details: A dinucleotide substitution was introduced at codon 588 (exon 14), converting the lysine codon to an arginine codon. While protein was detected at wild-type levels in homozygous mutant mice, the absence of lysine 588, a conserved residue of the kinase subdomain II, resulted in an ablation of catalytic activity. The targeting construct also included neo and tk selection genes in the 3' untranslated region.
(J:84535)