Figure 5

SHED-CM and DPSC-CM promote the neurite extension of CGNs on CSPG or MAG.

CGNs were plated on PLL (A), PLL /CSPG (B–F), or PLL /MAG (G–K), with the CM indicated at the top of each panel. CGNs plated with SHED-CM or DPSC-CM extended their neurites on the CSPG- (C and D) and MAG-coated (H and I) dishes, while BMSC-CM and fibroblast-CM (Fibro-CM) elicited only marginal extension on CSPG (E and F) and MAG (J and K). Quantification of the neurite length of CGNs plated on CSPG (L) and MAG (M). The y axis indicates the neurite length. Data represent the average measurements for each cell type from 3 independent donors. This set of experiments was performed 3 times and yielded similar results. Error bars represent SD. *P < 0.05. Scale bar in A is 100 μm.