miRge

Assists in understanding and analyzing microRNA sequencing data. miRge consists of a multiplexing method to align microRNAs (miRNAs) and other RNA species to expressed libraries. It includes among others miRNA annotation, A-to-I analysis, or miRNA detection. It uses both miRNA hairpin sequence structure and composition of isomiRs resulting in a more specific capture of potential miRNAs.

miRge citations

(2)

library_books

Current Research on Non Coding Ribonucleic Acid (RNA)

2017

Genes

PMCID: 5748684

PMID: 29206165

DOI: 10.3390/genes8120366

[…] e regulation of the positive transcription elongation factor P-TEFb [].Many miRNA processing pipelines have been established for utilizing HTS data. The major pipelines include: Oasis [], Chimira [], miRge [], and TIGER [], etc. Several tools have also been developed to detect isomiRs such as SeqBuster [], isomiRID [] and DeAnnIso []. One tRNA detection tool, tDRMapper [], is currently available. […]

library_books

Uptake of dietary milk miRNAs by adult humans: a validation study

2016

F1000Res

PMCID: 4857747

PMID: 27158459

DOI: 10.5256/f1000research.9200.r13516

[…] pooled samples, representing the five donors at time points 0, 3, 6 and 9 hours post-milk intake. Each sample had between 17.9 and 21 million total reads (
). We analyzed the sample by three methods: miRge
, Chimira release 1.0
, and direct mapping via Bowtie v1.1.2 to mature miRNAs. The percent miRNAs were between 96.6 and 99.1% for samples at T0, T6, and T9. However, at T3, the % aligned miRNAs […]