Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Thermo Scientific NeutrAvidin™ Protein is avidin that has been deglycosylated to prevent nonspecific lectin-binding. NeutrAvidin Protein has a near-neutral pI (6.3), resulting in lower nonspecific interactions in certain applications compared with streptavidin. NeutrAvidin Protein has an additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates nonspecific binding due to the RGD binding domain of adhesion receptors present in a variety of cells. Pierce NeutrAvidin Coated Plates are available in clear, white and black plates for use with colorimetric, chemiluminescent and fluorescent detection methods, respectively.

Highlights:

Easy and gentle immobilization of biotin-containing conjugates

Lowest nonspecific binding properties of all biotin-binding proteins

Thermo Scientific NeutrAvidin Biotin-Binding Protein has no carbohydrate and an isoelectric point of 6.3

No denaturing of the protein component of a conjugate upon binding to the plate