Applications/Dilutions

Dilutions

Western Blot 1:2000

Immunohistochemistry 1:10-1:500

Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Human, Monkey, Mouse, Rat. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Buffer

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Purity

Immunogen affinity purified

Alternate Names for HES-1 Antibody (7B9)

BHLHB39

bHLHb39hairy homolog (Drosophila)

Class B basic helix-loop-helix protein 39

FLJ20408

Hairy and enhancer of split 1

hairy and enhancer of split 1, (Drosophila)

Hairy homolog

Hairy-like protein

HES1

HES-1

hHL

HL

HRY

HRYHHL

transcription factor HES-1

Background

This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Video Protocols

FAQs for HES-1 Antibody (NBP2-01595). (Showing 1 - 1 of 1 FAQ).

What is the molecular weight of the band targeted by NBP2-01595 (Hes 1) in WB? The two images showed bands of different location. It's confusing.

Yes, you are correct, that WB image from 9 different cell lines is very confusing. I did mention this to our lab and they are going to re-test the antibody in those cell lines. Upon receiving the WB image, I will forward it to you and our team at Novus will update the image on the datasheet as well. I do apologize for the confusion, please feel free to contact me if you have more questions or concerns.

Blogs on HES-1

Contact Information

Product PDFs

Calculators

Concentration Calculator

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

Volume

=

Mass

÷

Concentration

Review this Product

Be the first to review our HES-1 Antibody (7B9) and receive a gift card or discount.