Music is an important part of every known culture, and its universality raises the question of a possible biological basis. Musical disorders, such as congenital amusia, offer compelling insight into these roots. In order to examine the genetic basis of this phenotype, we used a classical twin study paradigm. Our study had two main goals: (a) investigate if identical (monozygotic; MZ) co-twins perform more similarly on auditory and musical tasks than non-identical (dizygotic; DZ) co-twins and (b) explore the twins’ musical environments in order to better understand the contribution of environmental versus genetic factors in the differences underlying musical abilities. In addition, we sought to replicate previous investigations that demonstrated impaired pitch processing but intact rhythm perception in amusic participants. To do so, we tested eight pairs of MZ and six pairs of DZ twins in which at least one of the co-twins was potentially amusic. Participants completed an online amusia test, a pitch detection task and a singing task. We observed no performance or musical environment differences between MZ and DZ twins, suggesting that musical abilities might be more influenced by the twins’ shared environment than by genetic factors. In addition, and as reported in previous studies, amusics made pitch processing errors in both perception and production, but rhythmic perception was preserved. Future studies, particularly those with access to larger twin samples, will be able to further elucidate the roles of environmental and genetic factors in the amusic phenotype.