Ajax were as good as out following their 3-0 home defeat to Salzburg and things got little better for them in the second leg as they lost 3-1.

The Austrians scored three second-half goals through a Mike van der Hoorn own goal, Sadio Mane and Jonathan Soriano. Davy Klaassen grabbed a late consolation for Ajax.

Salzburg play Basel in the last 16 after the Swiss side won 3-0 at home to Maccabi Tel-Aviv with a Valentin Stocker strike and a Marco Streller brace earning them an easy win.

Lazio were beaten 4-3 on aggregate by Ludogorets Razgrad after they threw away a two-goal lead and conceded an 89th-minute equaliser in a 3-3 draw in Bulgaria.

The Romans looked to have turned around their home-leg deficit when goals from Keita Balde, who scored inside the opening 18 seconds, and Brayan Perea after 54 minutes put them 2-0 up on the night.

Roman Bezjak and Hristo Zlatinski brought it back to 2-2, only for Miroslav Klose to put Lazio back in front after 83 minutes.

But 85th-minute substitute Juninho Quixada struck four minutes after coming on to put Ludogorets through.

Juventus sent up a last-16 meeting with Serie A rivals Fiorentina. Antonio Conte's men won 2-0 in Turkey at Trabzonspor thanks to first-half goals from Arturo Vidal and Dani Osvaldo to seal a 4-0 aggregate victory.

La Viola also held a healthy lead from the first leg against Esbjerg, 3-1, and completed the job with a 1-1 home-leg draw.

Josip Ilicic scored a free-kick early in the second half and Mikkel Vestergaard's well-taken stoppage-time goal was mere consolation.

Benfica found things far easier at home to PAOK Salonika, winning 3-0 to go with their 1-0 success in Greece.

A sending-off also proved pivotal in Lisbon, Konstantinos Katsouranis seeing red for PAOK in the 69th minute.

Nicolas Gaitan put Benfica ahead a minute later, Rodrigo Lima adding a second from the spot in the 79th minute and Lazar Markovic adding another a minute after. They face Tottenham next.

Valencia booked a tie with Ludogorets, a 0-0 draw at home to Dynamo Kiev sending them through 2-0 on aggregate.

Lyon won 1-0 at home to Chornomorets to set up a tie with Plzen thanks to a Alexandre Lacazette goal.

The final last-16 tie will be between AZ Alkmaar and Anzhi Makhachkala.

AZ, 1-0 up from the first leg against Liberec, went through after a 1-1 home draw, while Anzhi won 2-0 at Genk.