Overview

MeSH

Adenoviridae

Animals

Antibodies

Defective Viruses

Female

Gene Transfer Techniques

Genetic Vectors

Locomotion

Mice

Mice, Inbred BALB C

Motor Activity

MeSH Major

Cocaine

Substance-Related Disorders

Vaccines

abstract

Based on the concept that anticocaine antibodies could prevent inhaled cocaine from reaching its target receptors in the brain, an effective anticocaine vaccine could help reverse cocaine addiction. Leveraging the knowledge that E1(-)E3(-) adenovirus (Ad) gene transfer vectors are potent immunogens, we have developed a novel vaccine platform for addictive drugs by covalently linking a cocaine analog to the capsid proteins of noninfectious, disrupted Ad vector. The Ad-based anticocaine vaccine evokes high-titer anticocaine antibodies in mice sufficient to completely reverse, on a persistent basis, the hyperlocomotor activity induced by intravenous administration of cocaine.