We have overcome the difficulty of measuring
blood-brain barrier (BBB) permeability in mice longitudinally by combining T1
mapping and using i.p. administration of Gd-DTPA. We have successfully
quantified BBB permeability from the signal changes associated with uptake of
Gd-DTPA following traumatic brain injury for 2weeks using T1 map. Results
show that significant entry of Gd-DTPA into the brain was evident in the
injury site at P3&P7 but almost return to normal level at P14. This study
demonstrates that the technique can be employed in longitudinal study of BBB
permeability in mice brain in vivo.