Summary

Secondary Antibodies

Negative Isotype Controls

Useful Reagents

Positive Controls

Histology Controls

More Images

References

Reviews

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Rabbit anti LCK (pTyr394) antibody recognizes LCK, also known as tyrosine-protein kinase Lck, leukocyte C-terminal Src kinase, lymphocyte cell-specific protein-tyrosine kinase or p56-LCK, when phosphorylated at tyrosine 394. LCK is a member of the Src family of tyrosine kinases. The T cell antigen receptor (TCR) depends on LCK to initiate signaling.

LCK is autophosphorylated at tyrosine 394 in response to TCR engagement (Wright et al. 1994). Phosphorylation of LCK at tyrosine 394 leads to phosphorylation of CD3 and the gamma chains of the TCR as part of T cell activation.

Intended Use

Target Species

Human

Species Cross-Reactivity

Target Species

Cross Reactivity

Mouse

Expected from Sequence

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Reconstitution

Preparation

Antiserum Preparation

Antiserum to LCK (pTyr394) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Store at -20oC Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name

Yes

No

Min Dilution

Max Dilution

Western Blotting

1/500

1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice

Recommended Protocol

ELISA

Immunohistology

Histology Positive Control Tissue

Immunofluorescence

Western Blotting

Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.