1. CloneTracker XP™ Lentiviral Barcode Libraries

Cellecta’s CloneTracker XP™ Lentiviral Barcode Libraries are pooled expressed barcode libraries that enable the tracking and profiling of individual clones derived from a population of cells using either PCR/NGS of barcodes integrated in genomic DNA or RNA-Seq of transcribed barcodes. Pre-made pooled libraries consisting of either 1 million or 10 million barcoded constructs are available, with each construct expressing a unique barcode sequence.

When a cell population is transduced with a CloneTracker XP Barcode Library, the barcodes integrate into the genomic DNA of the cells. The result is a starter founder population where almost every cell has a different barcode integrated into its DNA. Since the barcode is stably integrated, it is passed onto any cell progeny when genomic DNA is replicated. This feature enables identification of all the progeny derived from each individual cell.

When used in conjunction with single-cell RNA-Seq, the expressed barcode may be used to identify expression profiles and activated genes in different cells so that distinct clonal populations of cells from a single progenitor, as well as sub-populations of cells with distinct pathway activations or expression profiles, can be readily identified.

Additionally, CloneTracker XP barcode libraries, similarly to the standard CloneTracker (CellTracker) 50M Barcode Library, allow researchers to track cell proliferation over the course of an experiment. For instance, it is possible to:

The protocols included provide guidelines for packaging the CloneTracker XP Barcode Library into VSV-G pseudotyped lentiviral particles, transduction of target cells, and the preparation of barcodes derived from transduced cells for NGS, enumeration, and analysis.

Please read the entire user manual before proceeding with your experiment. Also, please note that, when working with pseudoviral particles, you should follow the recommended guidelines for working with Biosafety Level 2 (BSL-2) materials.

*Click the ⤓ Download as PDF link located at the bottom of the left menu to download the PDF version of this user manual.

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