The test consists of a membrane strip, which was pre-coated with rabbit anti-Strep A antibody on the test band region and goat anti-rabbit antibody on the control band region. The mixture then moves chromatographically on the membrane to the immobilized rabbit anti-Strep A antibody at the test band reagent. If Strep A antigen is present in the specimen, a colored sandwich of solid phase - Strep A antigen - gold conjugate is formed on the test band region.

The mixture then migrates through the membrane by capillary action and interacts with reagents on the membrane. If there is sufficient Strep A antigen in the specimen, a colored band will form at the test region of the membrane. The presence of this colored band indicates a positive result, while its absence indicates a negative result.

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