ROR1 Serves as Predictive Surrogate Marker for Cardiomyocyte Formation(A) Volcano plot comparing cardiac and presomitic mesoderm on day 3 of differentiation. Key genes for each condition are highlighted in respective colors, either grouped in categories (, , , ) or individually (, ). Note the detection of ROR1 () in the cardiac mesoderm by three independent probes and the minor difference for established surface markers (), n = 4 independent experiments.(B) Representative FC histograms for ROR2, CD13, and ROR1 on day 3 with () and without () IWP2 treatment on day 1.(C) Gene expression in the gastrulating mouse embryo () indicates Ror1 expression (right panel) in the anterior-to-mid PS region whereby T expression (left panel) increases toward the posterior PS.(D) Pattern of mesodermal surface markers in human fetal tissues () indicates high ROR1 levels in the heart.(E) Representative FC on day 3 for NCAM/EpCAM, CD13/ROR2 and CD13/ROR1 with/without addition of IWP2 on day 1 and corresponding CM content (cTNT, SA, MHC) on day 10.(F) Correlation of ROR1+/CD13+ on day 3 and respective CM content on day 10 based on three different cell lines; each dot represents an independent biological repeat. r denotes Pearson's correlation coefficient. Dotted lines denote 95% confidence interval.See also .