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A set of over 1200 KASP™ SNP genotyping assays for maize has been developed for CIMMYT’s Global Maize Program and the Generation Challenge Programme. The assays have been applied to over 500 projects with CIMMYT and CGIAR globally. Through LGC, these assays are now available to the maize breeding community to accelerate development of improved varieties by marker-assisted selection (MAS).

Development of the maize genotyping library

The Zea mays SNP markers were developed from the mapping data against the B73 reference genome (Jones et al., 2009) and a SNP mining study from EST sequences (Batley et al., 2003). The SNPs were mapped against 284 maize recombinant inbred line populations – IBM (B736Mo17) and LHRF (F26F252), 24 proprietary Pioneer varieties and 60 Elite varieties, including North American and European lines, and 3 tropical lines.

Advantages

Library includes over 1200 functionally validated SNP markers

Cost-effective and robust KASP genotyping technology

Flexible - pick and choose the assays that are relevant to your project based on genome and chromosome location

KASP assays are easy to run in your own laboratory or can be used in a genotyping service project

Ordering

The Maize KASP Assays can be provided as part of our genotyping service or as reagents to run KASP genotyping in your own laboratory.

With our Assay Search Tool, you can search for specific assays, export your selection, pick the required amount of KASP Master mix, and request a quote. To place an order straightaway for all assays in your cart, please use the “export assay cart” function and forward the exported Excel file together with your purchase order to orders.uk@lgcgroup.com