Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Applications

Approved:

Flow Cytometry

Specificity and Use

Immunogen:

TRAIL-R3 / DCR1 antibody was raised against human DcR1

Usage:

The DJR3 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral leukocytes and human DcR1-transfected cells. This can be used at 20 ul (1 ug)/per test. A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

Presentation:

PBS, pH 7.2, 150 mM sodium chloride, 0.09% sodium azide, 0.2% BSA

Storage:

Store at +4°C. Do not freeze. Product is photosensitive and should be protected from light.

Description: Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumor cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL. Two decoy receptors for TRAIL have been identified and designated DcR1/TRID/TRAIL-R3/LIT and DcR2/TRAIL-R4/TRUNDD.

Flow Cytometry

Surface staining of human DcR1 transfected cells with anti-human DcR1 (DJR3) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.