A web based environment designed to integrate bioinformatics-like information for the analysis of protein interactions and quaternary structure modellling. 3DIANA is specially targeted to the cases in which the electron microscopy map resolution is medium or low and additional experimental structural information is scarce or even lacking. In this way, 3DIANA statistically evaluates proposed/potential contacts between protein domains, presents a complete catalog of both structurally resolved and predicted interacting regions involving these domains and, finally, suggests structural templates to model the interaction between them. The evaluation of the proposed interactions is computed with DIMERO, a new method that scores physical binding sites based on the topology of protein interaction networks, which has recently shown the capability to increase by 200% the number of domain-domain interactions predicted in interactomes as compared to previous approaches.

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Funding source(s)

This work was supported by the Instituto de Salud Carlos III, project number PT13/0001/0009 funding the Spanish National Institute of Bioinformatics, the Spanish Ministry of Economy and Competitiveness through grants AIC-A-2011-0638 and BIO2013-44647-R and the European Union (EU) and Horizon 2020 through grant CORBEL (INFRADEV-1-2014-1 - Proposal: 654248).