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Your time frame is too short. Given the meal you had, even if fasted, you could still be digesting/absorbing for a couple more hrs.

During the time frame I would not expect much change in rate glucongenisis to occur. Certanly nothing that a capillary sample would detect with much accuracy.

The meal was off mixed compostion.

I certanly would not expect to see a change in urinary protein content.

n=1

Its a valiant effort but even using varied biochem methods and calculations in labs its not a simple thing to get right.

Again, I believe your thesis to be correct! There is no arbitary number that needs to be set against the amount of protein that we can absorb from a feed. It's more a matter of 'time' than much else. There off course is a debate about the increase in protein balance that can occur from a single feeding or protein. Protein balance can indeed be at basline inspite of significant concentrations of cirulatory amino acids.

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Cus smoking and tripping is all that you do. Ohhhh yeaahhh

It isn't supposed to prove anything on a rigorous scientific level. This is just something I live with day in and day out. People shit their pants in fear of not being optimal. Screw optimal, eat a steak.

Now that my rant at the masses is over, I totally agree with what you are saying. There are major studies done on this sort of thing with people who have lots of money and equipment at their disposal. This was simply an attempt to show that the idea that your body implodes if you get more than a very low amount of protein is false. Can I prove it with peer reviewed double blind studies of over 100 participants who are also athletes? Not really. But my traps will support my conclusion

It is a good discussion to have, in particular because I value science and rigor greatly. I studied chemistry and mathematics in a past life before I sold out and went the route of computer science, and I have a soft spot for a good study and quality research. But every now and then a little bit of anecdotal evidence on a topic that has been blown way out of proportion is a good thing. With words like metabolic damage, optimal protein synthesis, de novo lipogenesis, and all natural anabolic amplifier being bandied around by people with heavy hitting advertising departments, this is an attempt at a return to sanity, with some numbers for the generation who can't actually read or comprehend them, but requires numbers and charts before they believe anything.

Actually one other thing I wanted to mention. I may have said something about it in the article, but I have scarring damage to my kidneys from an auto-immune disease. My doctors demand that I eat low protein and say that high protein will destroy my kidneys. In the old days, I would avoid dietary protein before doing a urinalysis, but over time discovered it didn't make a bit of difference.

The only time 30g or any spread pattern matters is when you are mainly getting whey as your main protein source in which case you would want to spread your shakes out since that's excess amounts of fast acting proteins are oxidized easily. The Protein Book by Lyle McDonald is a great read by the way....goes over everything.

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Experimentation is the word of the day here, and you're going to be like a mad scientist, tinkering with your diet until you've turned yourself into a massive, world-destroying robot bent on nothing short of the subjugation and destruction of the entire human race.
-Jamie Lewis (Chaos&Pain)

The only time 30g or any spread pattern matters is when you are mainly getting whey as your main protein source in which case you would want to spread your shakes out since that's excess amounts of fast acting proteins are oxidized easily. The Protein Book by Lyle McDonald is a great read by the way....goes over everything.

Every time I see one of your posts I have this urge to eat a large pizza.