9
9 Can run on-instrument using MiSeq Reporter or in cloud with BaseSpace Both analysis pipelines use the same classification algorithm and taxonomic database. –The classification algorithm is a high performance implementation of the published RDP Naïve Bayesian Classifier (http://dx.doi.org/10.1128%2FAEM.00062-07)http://dx.doi.org/10.1128%2FAEM.00062-07 –The database is an Illumina-curated version of the GreenGenes Consortium 16S rRNA database. Redundant sequences and entries with missing or partial labels are removed. Provides fast, high-accuracy species-level taxonomic classifications Uses full length of Illumina paired-end reads Outputs: PDF reports, raw data (CSV), interactive visualizations Taxonomic classification

20
20 BaseSpace: Plug and Play Genomic Cloud Solution All you need is an internet connection

21
21 How Is BaseSpace Being Used World Wide? Users & Growth Bioinformatics Cloud Computing Service Illumina Begins Streaming MiSeq Data to the Cloud October 2011 Illumina Begins Data Sharing in the Cloud December 2011 Illumina Begins Streaming HiSeq Data to the Cloud November 2012 BaseSpace Commercial (Supported) Release May 2013 Over 20,000 Instrument Runs Streamed to BaseSpace December 2012 Over 40,000 Instrument Runs Streamed to BaseSpace April 2013 General Availability of BaseSpace to all HiSeq instruments July 2013 Over 60,000 Instrument Runs Streamed to BaseSpace, and Over 10,000 Apps Run September 2013