Product Description

Small GTPases are a super-family of cellular signaling regulators. The small Ras-like GTPase Rap1 is an evolutionary conserved protein that originally gained interest because of its capacity to revert the morphological phenotype of Ras-transformed fibroblasts. Rap1 is regulated by a large number of stimuli that include growth factors and cytokines, but also physical force and osmotic stress. Rap1 was shown to regulate multiple basic cellular processes. The best studied aspect of Rap1 function in endothelial cells involved its role in regulation of cell-cell junction formation and remodeling.

Currently there is no direct assay to measure the activation of Rap1 GTPases.

NewEast Biosciences Rap1 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rap1-GTP, but not Rap1-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.

These anti-Rap1-GTP monoclonal antibodies can also be used to monitor the activation of Rap1 in cells and in tissues by immunohistochemistry.