Antibody purification using protein A / G / L beads

Protein A, protein G and protein L are native and recombinant proteins of microbial origin that bind to mammalian immunoglobulins (Ig). Protein A and protein G bind with high affinity and specificity to the Fc region of immunoglobulins from various mammalian species, most notably the IgGs. Protein L binds immunoglobulins via the kappa light chain. These antibody-binding proteins can be used to purify, immobilize or detect immunoglobulins. These proteins (i.e. protein A, protein G, protein L) are typically attached onto a solid support such as
sepharose
or
magnetic
beads.