Combined Protease and Phosphatase Inhibitors

These combined protease and phosphatase inhibitor cocktails and tablets contain chemical compounds that target serine, threonine, and tyrosine phosphatases, as well as serine, cysteine, and aspartic acid proteases, as well as aminopeptidases. Metalloproteases are inhibited by the addition of EDTA, which is available in a separate vial in the liquid format, but included in the tablet format. These broad-spectrum inhibitor formulations prevent protein degradation and preserve phosphorylation simultaneously, helping provide complete protection in a single solution or tablet.

|Figure 1. Effective inhibition of protease and phosphatase using the combination tablet.| Using the tablet formulation that inhibits protease and phosphatase activity, the degree of enzyme inhibition was determined. A quenched-fluorescent substrate with pancreatic tissue extract was used and fluorescence measured after substrate cleavage. The degree of inhibition is indicated. Panel A: Protease assay reactions were incubated for 2 hours and 37°C. Panel B: Acid phosphatase assay reactions were incubated for 1 hour at 37°C.