The absorbance data did not show a trend. The thiol-DNA/ThT/AuNP sample itself was the faintest shade of purple, which may be one reason we did not observe a distinguishable peak. Because of this, we decided to re-do the thiol-DNA/ThT reaction using a higher concentration of DNA (See Part 3 of the procedures). This allowed us to work with a higher concentration of gold nanoparticles since it's concentration is based off of the concentration of DNA used.

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==Notes==

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* We are still having trouble observing the gel under UV light. It is difficult to even see streaks due to the ladders. Because of this, we are going to focus on making a gel using different volumes of the ladders.

Because we are reacting the ThT with the DNA prior to reaction with the gold nanoparticles, it is important to account for an extra dilution that minimizes the concentration of the DNA. We are looking for the DNA to be 75x higher than the concentration of the gold nanoparticles (19.4nM x 74 = 1455nM). In order to have this concentration of DNA by the time it is ready to be mixed with the AuNP, it was calculated that we need to start with a 5.82μM solution

1) Dilute stock DNA (53.1μM) to 5.82μM

53.1μM DNA (x) = (500μL) (5.82μM)

X = 54.8micL in 445.2 mic buffer

2) Mix 250μL thiol-DNA with 250μL 5μMThT

3) Heat at ~75°C for 25 minutes

4) Cool to room temperature

Data

Observations

The absorbance data did not show a trend. The thiol-DNA/ThT/AuNP sample itself was the faintest shade of purple, which may be one reason we did not observe a distinguishable peak. Because of this, we decided to re-do the thiol-DNA/ThT reaction using a higher concentration of DNA (See Part 3 of the procedures). This allowed us to work with a higher concentration of gold nanoparticles since it's concentration is based off of the concentration of DNA used.

Notes

We are still having trouble observing the gel under UV light. It is difficult to even see streaks due to the ladders. Because of this, we are going to focus on making a gel using different volumes of the ladders.