IHC image of Aconitase 2 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110321, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IHC-Fr image of Aconitase 2 staining on six month old rat muscle tissue using ab110321(1:1000).4% PFA fixed sections were incubated in 0.3% PBS-Tween for 1h. The sections were then incubated with ab110321 (1:1000) overnight at +4°C. The secondary antibody Alexa Fluor® 568 donkey anti-mouse was used at a 1/1000 dilution for 1h. DAPI was used at 1.43µM

Thank you for contacting us. Both ACO2 antibodies cross-react with mouse, however ab110320 is more suitable for immunoprecipitation. Ab110321 is a good WB antibody. In this particular case, I would recommend using ab110320 then. I hope this information...