Major instrumentation includes Waters Xevo TQ-XS Tandem Mass Spectrometer with ACQUITY UPLC I-Class liquid chromatography system, gas chromatograph (GC) with a mass spectrometer: Agilent 5977A, and with a nitrogen phosphorus detector: Agilent 7890B. This instrumentation is capable of a wide variety of nicotine and tobacco product testing and tobacco-specific biomarker assessment. Application examples include determination of nicotine, carcinogens and flavorings in tobacco products and electronic cigarettes, as well as tobacco-specific biomarkers in biofluids.