DNA Polymerase & PCR Kits—Titanium Taq

Titanium Taq DNA Polymerase is a highly sensitive, robust enzyme for use in all PCR applications. It’s ideal for the PCR amplification of any DNA template, including bacterial and plasmid DNA, cDNA, and complex genomic DNA. Titanium Taq includes TaqStart Antibody, which provides an integrated hot start for increased specificity and yield (1).

Titanium Taq DNA Polymerase lacks the 5'-exonuclease activity of wild-type Taq, which makes it more robust than other Taq polymerases and allows it to amplify highly complex DNA mixes. This novel enzyme also contains carefully engineered amino acid substitutions that increase its solubility, making it the most sensitive PCR polymerase available (2).

Amplify targets of up to 2 kb from highly complex templates, such as mammalian genomic DNA. Targets of up to 4 kb can be readily amplified when the template is a moderately abundant cDNA or a less complex genomic DNA

Integrated Hot Start for Higher Specificity

Titanium Taq comes preblended with TaqStart Antibody. This hot start antibody, which is quickly inactivated at the onset of thermal cycling, blocks polymerase activity during the PCR reaction set-up, preventing the formation of primer-dimers and other artifacts prior to thermal cycling. This integrated hot start feature allows for higher specificity than other traditional Taq-based methods. It is especially useful for multiplex PCR because it limits the number of mispriming events caused by multiple primer sets, which can result in spurious PCR products or smeared gel bands.

Applications

References

Kellogg, D. E. et al. (1994) BioTechniques 16(6):1137–1137.

Barnes, W. M. (1992) Gene112(1):29–35.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.