アプリケーション

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション

Abreviews

特記事項

ICC/IF

Use at an assay dependent concentration. PubMed: 20818377

IHC-Fr

1/1000 - 1/5000.

IHC-P

1/500 - 1/1000.

ELISA

Use at an assay dependent concentration.

WB

Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 84 kDa.

Flow Cyt

1/20 - 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

機能Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

関連疾患Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.

Anti-Myeloperoxidase antibody [2C7] 画像

Flow Cytometry-Myeloperoxidase antibody [2C7](ab25989)

Overlay histogram showing HeLa cells stained with ab25989 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25989, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

DISCOUNT CODE: xxxxxxx
Expiration date: 2012-04-20
As discussed I have generated a discount code for ab25989. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for staining ...

Thank you for contacting us and considering our testing discount with ab25989. As the sequence of human Myeloperoxidase shares 86% homology with the pig protein we would predict that ab25989 would be able to recognise this protein.
I can issue a te...

Thank you for your enquiry regarding ab25989 and your interest in our products.
The immunogen was full-length myeloperoxidase. The antibody recognizes an epitope on the heavy chain, but the exact epitope location has not been mapped.

Ab25989 recognizes an epitope on the heavy chain of myeloperoxidase. The protein is a multimer of 2 heavy chains (55kDa) and two light chains (15kDa), the heavy chains being linked by a disulfide bond. In Western blot under non-reducing conditions, t...