IgD is the major antigen receptor isotype on the surface of most peripheral B-cells, where it is coexpressed with IgM. The membrane-bound IgD (mIgD) induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. Soluble IgD (sIgD) concentration in serum below those of IgG, IgA, and IgM but much higher than that of IgE. IgM and IgD molecules present on B cells have identical V regions and antigen-binding sites. After the antigen binds to the B-cell receptor, the secreted form sIgD is shut off. IgD is a potent inducer of TNF, IL1B, and IL1RN. IgD also induces release of IL6, IL10, and LIF from peripheral blood mononuclear cells. Monocytes seem to be the main producers of cytokines in vitro in the presence of IgD.

Catalog #

031042

Applications

Suitable for use in Western Blot, ELISA.

Recommended Dilution

ELISA: 1:1,000

Western Blot: 1:100~250

Storage and Stability

May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Clone Type

Monoclonal

Isotype

IgM

Clone No

607CT8.4.4

Host

Mouse

Source

Human

Concentration

As reported

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

Purity

Purified by ammonium sulfate precipitation.

Immunogen

IGHD antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 37-64 amino acids from the N-terminal region of human IGHD.

Specificity

Human

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.