Bitter gourd (Momordica charantia) were analyzed for their major nutrient contents as well as Vitamin C, minerals and phytochemical constituents. The selected varieties were Charantia C.B. Clarke, Muricata (Willd.), Hybrid green and Hybrid white karala. The result revealed the presence of nutrient constituent among the varieties comprising Total sugar (0.114±0.01% to 0.22±0.02%), Starch (0.74±0.01% to 5.3±0.04%), Total protein (1.17±0.01% to 2.4±0.02%), Fat (0.3±0.02 to 0.8±0.02%) and Vitamin C (9.41±0.26 to 13±0.57mg%). This vegetable is good source of minerals such as Mg, Ca, S and Cu. The fruits contained calcium (0.55±0.30 to 7.0±0.25 mg%) and sulpher (44.9±0.38 to 72.4±0.51 ppm). All the varieties were found to contain tannin, flavonoids, terpenoids, cardiac glycosides, triterpin and sterol, resin, amino acid and phenolic compounds except coumarin and free anthraquinone. Based on the findings from this study, bitter gourd (Momordica charantia), although a bitter known vegetables but has enormous nutritional potentials.

Nutrition is the science of food, the nutrients and other substances there
in and their action, interaction and balance are related to health and diseases
(Clamp, 2007). Now a days malnutrition is one of the
common word in todays world.

A large number of populations in Bangladesh have been suffering from malnutrition.
There are many kinds of plants available in Bangladesh which are rich in nutrients.
For the ignorance and proper knowledge of the people, they do not know the nutritive
value of most of vegetables and fruits. Green vegetables occupy an important
place among the food crops as they provide adequate amounts of many vitamins
and mineral for humans. In particular, the consumption of whole-plant foods
slows digestion and allows better absorption. A more favorable balance of essential
nutrients per calorie, results in better management of cell growth, maintenance
and mitosis, as well as better regulation of appetite and blood sugar (Fuhman
and Oz, 2003). They are rich sources of carotene, ascorbic acid, folic acid
and minerals like calcium, iron and phosphorus (Fasuyi, 2006).
In addition they contain anti nutrients which reduce their bioavailability (Akindahunsi
and Salawu, 2005). Momordica charantia, or bitter melon, is a common
food in Indian cusine and is also used as a medicine to treat a wide array of
conditions in the tropical regions in which it grows. The leaves and fruit of
bitter gourd have been used in folk medicine traditions of China, India, Africa
and the West Indies since ancient times. Bitter gourd has important role as
a source of carbohydrate, proteins, vitamins, minerals and other nutrients in
human diet (Ali et al., 2008) which are necessary
for maintaining proper health. The presence of secondary metabolites such as
alkaloids, saponins, tannins, glycosides and cardiac glycosides in the M.
charantia may contribute to its medicinal value. Some of these compounds
are well documented to exhibit hypoglyceamic activity in animals. Saponins inhibit
Na+ efflux leading to higher Na+ concentration in cells,
thereby activating a Na+- Ca2+ antiport. This effect produces
elevated cytosolic Ca2+ which strengthens the contraction of the
heart muscle and thereby reducing congestive heart failure (Schneider
and Wolfling, 2004). Bitter melon has been implicated experimentally to
achieve a positive sugar regulatory effect by suppressing the neural response
to sweet taste stimuli and also keep the body functions operating normally.
Other use of the plant include to expel intestinal gas, for tumors wound treatment,
rheumatism, malaria, vaginal discharge and the seeds are used to induce abortion
(Taylor, 2005). M. charantia leaves has been used
extensively in traditional medicine as a remedy for diabetes (Cefalu
et al., 2008; Nahas and Moher, 2009). Rahman
et al. (2005) reported that blood glucose level of experimental rat
was decreased significantly (8.96 to 4.82 mol L-1) when karala was
administered orally 200 mg kg-1 body weight and they suggested that
the active constituents of Momordica charantiamight be responsible for
anti-hypoglycemic activity.

Bitter gourd has also been used as a traditional medicine for several other
ailments, including dysentery, colic, fevers, burns, painful menstruation, scabies
and other skin problems. (Beloin et al., 2005).
Further, the different plant foliar extracts including bitter gourd (Momordica
charantia) was found to be effective against infestation of maize and rice
by two major pests (Rani and Devanand, 2011).

This is the main reason why this vegetable is used in a number of native medicines of Asia and Africa. Till now there is no such data available about the nutrient contents of karala produced in Bangladesh. This study has been under taken to obtain information regarding major and minor nutrient contents as well biologically important phytochemicals in Bitter gourd.

MATERIALS AND METHODS

Collection and identification of plant materials:Momordica charantiais commonly known as karala and Uchchhey in Bangladesh.
Four varieties of Momordica charantia, charantia C.B. Clarke, muricata
(Willd.), Hybrid green and Hybrid white were collected from Chittagong hill
tracts, Chittagong, Bangladesh. The study was carried out in 2009 in the Department
of Biochemistry and Biotechnology, University of Science and Technology, Chittagong,
Bangladesh Fig. 1. The varieties were identified in the Department
of Botany; University of Chittagong. The seeds were dissected from the vegetables
and removed the foreign materials. After then, the fruits (pericerp) were used
for experimental analysis. The fruit (pericarp) were air-dried for 5 days and
milled into powder with the aid of an electrical grinder. The ethanol extract
of each sample was prepared by soaking 300 g of dried powdered samples in 2
L pure ethanol for 24 h. The extracts were filtered using Whatman filter paper
No 42 (125 mm).

Procedure of nutrient analysis: The analysis of bitter gourd moisture
were carried out by the standard IUPAC (1979) and Ash were
determined by the standard method of AOAC (1995). The other
nutrients were determined using the established reported methods as reported:
total sugar and starch content by the anthrone method of Jayaraman
(1981), reducing sugar by dinitrosalicylic acid method of Miller
(1972). The water soluble protein by the method of Folin-Lowry of Lowry
et al. (1951) and Total protein by Micro-Kjeldahl method of AOAC
(1960) using 6.25 factor to calculate protein content from nitrogen content.
Vitamin C by the standard method of AOAC (1995) while Lipid
content by the method of Bligh and Dyer (1959).

Estimation of mineral content: The minerals present in fruits were analyzed
by the procedure as described in Analytical methods (Petersen,
2002).

Statistical analysis: The results generated from the analysis were subjected to statistical analysis using the Statistical Package for Social Sciences (SPSS) Version 15. Means were used for the analysis of the result.

RESULTS AND DISCUSSION

The amount of moisture, ash, total sugar, reducing sugar and starch content
present in four varieties of bitter gourd fruits are shown in Table
1. It appears from the table that the moisture content varies from 91.6
to 92.92% and the ash content ranges from 0.75-1.2%. The moisture content of
karala was reported to be 91.2% (Soomro and Ansari, 2005).
Varieties muricata contained higher amount of ash (1.2±0.02%) (Table
1).

Among the varieties examined the highest amount of total sugar was recorded from varieties muricata (0.216±0.02%) followed by varieties hybrid green karala (0.170±0.01%). All the four varieties contained negligible amount of reducing sugar. while the starch content ranges from (0.74-5.3%). Further, the varieties muricata contained significantly higher amount of starch contents (5.3±0.04) than all the other varieties. This study might be suggested that muricata (willd.) is also used as a good source of carbohydrate. It is not clear at this moment that why muricata variety contained more than double amount of carbohydrate as compared to that of the other varieties.

Table 2 represents the protein, fat and Vitamin-C contents of four varieties bitter gourds. It appears from the table that total protein content varies from 1.17 to 2.4%. Among the varieties examined the highest amount of protein was recorded in Muricata (2.4±0.02%) followed by varieties charantia (1.58±0.02%).

The protein content of karala was reported to 1.9% by Soomro
and Ansari (2005) which is very similar to our result but much higher than
that of unpeeled bitter gourd as reported (0.7 gm%) by Alvi
et al. (2003). No significant differences in water soluble protein
contents extracted from the four varieties bitter gourd samples was observed
in (Table 2). From this study it may suggest that both uchchhey
and karala are moderate sources of protein. The fat content of bitter gourds
ranges from 0.3 to 0.8%. Among them varieties, Muricata contained higher amount
(0.8±0.02%) than others. The least amount was recorded from hybrid white
karala (0.3±0.02%). Hybrid green karala contained 0.4% fat that is similar
to the result found by Soomro and Ansari (2005). This
finding indicates that bitter gourd is not good source of lipid.

Vitamin C content (16.2 mg/100 g) of varieties hybrid green karala recorded
in this study was found to be lower than that (33 mg/100 g) reported by USDA
Nutrient database. The Vitamin C content of four varieties ranges from 9.41
to 16 mg%. Among the varieties the highest amount of vitamin C was recorded
from varieties hybrid green karala followed by varieties muricata (13.0±0.57
mg%).

Comparative contents of water soluble protein, total protein,
fat and vitamin C content in the four varieties bitter gourd fruits

All values were the average of three determinations

Emebu and Anyika (2011) recorded vitamin C values of
Brassica oleracea (Borecole) 23.43 mg/100 gm Free radicals change to
cellular structures and other molecules can result in painful information, as
the body tries to clear out the damaged part. Vitamin C which prevents the free
radical damage that triggers the inflammatory cascade, is this also associated
with reduced severity of inflammatory conditions, such as asthma, osteoarthritis
and rheumatoid arthritis (Cohen et al., 2000).

The minerals contents of four varieties of bitter gourds are represented in
Table 3. Magnesium content of hybrid white karala is slightly
higher. The magnesium content ranges from 0.99 to 1.1 mg%. Of the minerals examined
calcium was found to be present (0.54-7.0 mg%). Variety charantia contained
higher amount of calcium (7.0±0.25 mg%) followed by muricata (4.3±0.18
mg%). On the other hand high amount of calcium was recorded in bitter gourd
137.69 mg/100 gm by Soomro and Ansari (2005). The actual
reason is unknown may be the environmental and soil factors are responsible
for this difference. Sulphar is an important nutrient element for human body.

Sulpher content of bitter gourd is 72.4±0.51 ppm, 60.05±0.45
ppm and 70.35±0.53 ppm in charantia and muricata, hybrid green karala
respectively. The least amount of sulpher was recorded from hybrid white karala,
44.95±0.38 ppm. Copper content of uchchhey is higher than karala and
its content in bitter gourds varied from 0.51 to 2.53 ppm. Among the varieties
the highest amount of copper was recorded from varieties muricata 2.53±0.01
ppm followed by varieties hybrid white karala so on. Soomro
and Ansari (2005) recorded copper values ofMomordica charantia (3.54
mg/100 g (3.54404 ppm).That is slightly higher than the varieties muricata.

The present data in Table 4 clearly indicated that the different types of bitter gourd contained almost the similar types of phytochemicals constituents i.e., functional groups which might be helpful for comparison and determination of the structure of active principles present in them.

Table 3:

Comparative contents of minerals in the four varieties bitter
gourd fruits

All values were the average of three determinations.

Table 4:

Phytochemical screening of ethanol extract of four varieties
of bitter gourd (edible part)

The presence of phenolic compounds in the plants indicates that these plants
might have anti-microbial agent. This ptoperties agreed with the findings of
Ofokansi et al. (2005) who reported that B.
pinnatum is effective in the treatment of typhoid fever and other bacterial
infections, particularly those caused by Staphylococcus aureus, Esterichia
coli, Bacillus subtilis, Pseudomonas aeruginosa, klebsiella aerogenes,
klebsiella pneunoniae and Salmonella typhi. From this data, it can
be suggested that both the karala and Uchchhey might be used in the treatment
of the placenta and navel of newborn baby which not only heals fast but also
prevent the formation of infections (Okwu, 2001, 2003).
The presence of an unidentified alkaloid with Rf.0.098 and 5-hydroxytryotamine
is also reported by Dhalla et al. (1961). It
may be noted that all the varieties of bitter gourd did not contain Coumarin
and Free anthraquinone glycoside. It was already reported that the extract of
crude bitter gourd is used for different disease such as disease of liver and
pancreas, anti-inflammatory, analgesic, reduces cholesterol level, promotes
appetite and supports blood sugar management for diabetes or people with high
risk of developing diabetes (Verma and Aggarwal, 1956).
Our present data clearly indicate that bitter gourd contain many types of phytochemicals.
Among them flavonoid is present in significant amount as well as also contain
substantial amount of resin and phenolic constituents. Further biological study
is needed to confirm the result more precisely.

CONCLUSION

In conclusion, the nutritional study suggests that Momordica charantia L. (Variety muricata) contains a good amount of nutritional components, though somebody dislike it due to bitter taste. All the varieties contained similar types secondary metabolite such as Tanin, Flavonoids, Terpenoids, Cardiac glycosides, Triterpin etc. It also reveals significant effects like antimicrobial activity as well as anti-inflammatory activities. Further study is necessary to elucidate the mechanisms behind the traditional effects.