The tone of the film is somewhat different than the first Trekkies, being slightly more respectful and less exploitative. For example, there are interviews with fans who are made to appear more "normal", and fans outside the USA are shown. The movie features reactions from fans regarding some of the more controversial aspects featured in the first film. There is a more balanced portrayal of the woman who attended a trial in her Starfleet uniform, making her appear more intelligent and rational than many of the "normal" people around her. There is also more respectful attention paid to the art of filk music, in what appears to be an apology for its brief (and humiliating) appearance in the first film.

The film shows fan film production in Germany and Minnesota, visits FedCon XI, and interviews members of Trek fan clubs from around the world including Unification in France, USS Brazil, and the Serbian Society for Science Fiction.

The film features an interview with Tony Alleyne, a fan from the UK who remodeled his apartment based on a Star Trek theme. At the time of filming, it was listed on eBay where he was asking US$2,000,000. In 2007, two years after the film's release, he sold it for £425,000 (about US$839,000). He had originally purchased the apartment in 1994 for £27,000. (BBC news videowbm)