Abstract

Objective To examine prospectively the association between muscular strength and mortality from all causes, cardiovascular disease, and cancer in men.

Design Prospective cohort study.

Setting Aerobics centre longitudinal study.

Participants 8762 men aged 20-80.

Main outcome measures All cause mortality up to 31 December 2003; muscular strength, quantified by combining one repetition maximal measures for leg and bench presses and further categorised as age specific thirds of the combined strength variable; and cardiorespiratory fitness assessed by a maximal exercise test on a treadmill.

Results During an average follow-up of 18.9 years, 503 deaths occurred (145 cardiovascular disease, 199 cancer). Age adjusted death rates per 10000 person years across incremental thirds of muscular strength were 38.9, 25.9, and 26.6 for all causes; 12.1, 7.6, and 6.6 for cardiovascular disease; and 6.1, 4.9, and 4.2 for cancer (all P<0.01 for linear trend). After adjusting for age, physical activity, smoking, alcohol intake, body mass index, baseline medical conditions, and family history of cardiovascular disease, hazard ratios across incremental thirds of muscular strength for all cause mortality were 1.0 (referent), 0.72 (95% confidence interval 0.58 to 0.90), and 0.77 (0.62 to 0.96); for death from cardiovascular disease were 1.0 (referent), 0.74 (0.50 to 1.10), and 0.71 (0.47 to 1.07); and for death from cancer were 1.0 (referent), 0.72 (0.51 to 1.00), and 0.68 (0.48 to 0.97). The pattern of the association between muscular strength and death from all causes and cancer persisted after further adjustment for cardiorespiratory fitness; however, the association between muscular strength and death from cardiovascular disease was attenuated after further adjustment for cardiorespiratory fitness.

Conclusion Muscular strength is inversely and independently associated with death from all causes and cancer in men, even after adjusting for cardiorespiratory fitness and other potential confounders.

Introduction

The role of muscular strength in the performance of activities of daily living and exercise, as well as in the prevention of chronic disease, is increasingly being recognised.12 Resistance exercise training increases muscular strength and is currently prescribed by major health organisations for improving health and fitness.3456 Likewise, cardiorespiratory fitness provides strong and independent prognostic information about the overall risk of illness and death in adults across a broad spectrum of ages.78910111213 This applies to apparently healthy people and to those with diabetes mellitus, hypertension, metabolic syndrome, and several types of cancer.14

Several prospective studies have shown that muscular strength is inversely associated with all cause mortality.15161718192021222324252627 All but two of these studies1826 assessed strength through a handgrip test, which involves relatively small muscle groups. In addition, most previous studies were short term follow-ups (4-6 years)15161727 or included only older adults (≥65 years).1720222326 The shorter term studies could have a problem with reverse causation, in that muscle weakness could be due to poor health. Moreover, it is unclear whether muscular strength is related to mortality in adults younger than 65.

Preliminary analyses in the aerobics centre longitudinal study (ACLS) showed an inverse association between a standardised measure of muscular strength and all cause mortality after controlling for several potential confounders, including cardiorespiratory fitness.28 We regarded this early report as suggestive because it included a small number of deaths and combined men and women in the analyses, which prevented a thorough investigation of disease specific and sex specific mortality risks. The present study extends our previous analyses and focuses on the importance of muscular strength as a predictor of death from all causes, cardiovascular disease, and cancer in a large cohort of men. We also examined the combined association of muscular strength and cardiorespiratory fitness with mortality risk. We hypothesised that men with higher levels of muscular strength would have lower rates of mortality than those with lower levels of muscular strength.

Methods

Between 1980 and 1989, 10265 men received a comprehensive medical examination and muscular strength tests at the Cooper Clinic in Dallas, Texas, United States, and were enrolled in the aerobics centre longitudinal study. Participants came to the clinic for periodic preventive health examinations and for counselling on diet, exercise, and other lifestyle factors associated with an increased risk of chronic disease. Participants thus were volunteers, were not paid, and were not recruited to the study as would be the case for a clinical trial. Many were sent by their employers for the examination, some were referred by their doctors, and others were self referred. The men in the analyses reported here were similar to the overall cohort of the study, with only minor differences in some clinical variables. For example, they were within 1 mm Hg for systolic and diastolic blood pressures, had virtually identical total cholesterol levels, high density lipoprotein cholesterol levels, and body mass index (data not shown). The death rate for the subgroup of men in this analysis is not significantly different from the overall cohort adjusted for age, risk factor, health status, and family history.

Participants performed a maximal graded treadmill test to assess their cardiorespiratory fitness. They were excluded from the present study if they were younger than 20 or older than 90 (n=91); they failed to achieve at least 85% of aged predicted maximal heart rate during the treadmill test (n=635); they had an abnormal resting or exercise electrocardiogram29 (n=550); they reported a history of myocardial infarction (n=48), stroke (n=6), or cancer (n=52); or they were underweight (body mass index <18.5 kg/m2; n=121). These criteria resulted in 8762 men aged 20-82, who were followed up from the date of the baseline examination until the date of death or 31 December 2003. Mortality surveillance was by the National Death Index, which has been shown to be an accurate method of ascertaining deaths in observational studies, with high sensitivity (96%) and specificity (100%).30

Participants were predominantly white, well educated, and belonged to the middle and upper socioeconomic strata. Participants provided written informed consent to take part in the follow-up.

Clinical examination

The clinical examination, as well as measures of muscular strength and cardiorespiratory fitness, are described elsewhere.83132 Briefly, the baseline examination was completed subsequent to an overnight fast of at least 12 hours and included a physical examination and an array of clinical measurements. Body mass index was computed from height and weight measured with a standard clinical scale and stadiometer. Systolic and diastolic blood pressure were recorded at rest, as the first and fifth Korotkof sounds, using standard auscultation methods.33 Hypertension was defined as a systolic blood pressure of 140 mm Hg or more, a diastolic blood pressure of 90 mm Hg or more, or previous doctor diagnosed hypertension. Concentrations of total and high density lipoprotein cholesterol, triglycerides, and glucose were measured using automated techniques in accordance with the standards of the Centers for Disease Control and Prevention lipid standardisation programme. Diabetes mellitus was defined as a fasting plasma glucose concentration of 7 mmol/l or more, previous doctor diagnosis, or use of insulin or other hypoglycaemic drugs. Hypercholesterolaemia was defined as a total cholesterol concentration of 6.2 mmol/l or more. Participants completed a questionnaire on medical history, which included a personal history of myocardial infarction, stroke, hypertension, diabetes, or cancer; a family history of cardiovascular disease; a family history of cancer; smoking status; alcohol intake; and physical activity (sedentary or active). Sedentary was defined as reporting no physical activity during leisure time in the three months before the examination.

We assessed muscular strength in the upper and lower body using a standardised strength testing protocol of variable resistance weight machines (Universal Equipment, Cedar Rapids, IA, USA).3132 Upper body strength was assessed with a one repetition maximum supine bench press, and lower body strength was assessed with a one repetition maximum seated leg press. Initial loads were 70% of body weight for the bench press and 100% for the leg press. After a brief rest period, we added increments of 2.27 kg to 4.54 kg until maximal effort was achieved for each lift, usually after five or fewer trials. Participants were able to lift the initial load at least one time. They were instructed on proper breathing and lifting form for each movement. The intraclass correlation coefficient for one repetition maximum bench press and leg press was 0.90 and 0.83, respectively, in a subgroup of 246 men who underwent two muscular strength assessments within one year.31

We computed a score for muscular strength by combining the standardised values of bench and leg presses. Each of these variables was standardised using the formula: standardised value=(value−mean)/standard deviation. We calculated the score separately for each age group (20-29, 30-39, 40-49, 50-59, and ≥60). The score for muscular strength was calculated as the mean of the two standardised scores (bench and leg presses). For analysis we used thirds of the age specific composite strength score.

We assessed cardiorespiratory fitness by a maximal treadmill test using a modified Balke protocol.834 Participants were encouraged to give maximal effort, and the test was stopped when participants reached volitional exhaustion or the doctor intervened for medical reasons. Participants were encouraged not to hold on to the handrails. The mean percentage of age predicted maximal heart rate (220−age) achieved during exercise was 101.6 (SD 6.2), indicating that most participants achieved maximal effort. Exercise duration using this protocol is highly correlated with measured maximal oxygen uptake (r=0.92).35 We estimated maximal metabolic equivalents (1 maximal metabolic equivalent=3.5 ml oxygen uptake/kg/min) from the final treadmill speed and grade.36 We dichotomised cardiorespiratory fitness as unfit (low) and fit (high) corresponding to the lower 20% and the upper 80%, respectively, of the age specific distribution of treadmill exercise duration in the overall population of the aerobics centre longitudinal study.73738394041 This approach was used in the current analysis to maintain consistency with our other reports and because we have found that low cardiorespiratory fitness, defined in this way, is a strong and independent predictor of mortality.

Mortality surveillance

We computed person years of exposure as the sum of follow-up time among decedents and survivors. The National Death Index was the primary data source for mortality surveillance, augmented with death certificates. The underlying cause of death was determined from the index or by a nosologist’s review of official death certificates obtained from the department of vital records in the decedent’s state of residence. Death from cardiovascular disease was defined by ICD-9 (international classification of diseases, ninth revision) codes 390 to 449.9 before 1999 and ICD-10 codes I00 to I78 during 1999-2003. Death from cancer was defined by ICD-9 codes 140 to 208 and ICD-10 codes C00 to C97.

Statistical analysis

We calculated the baseline characteristics of the study population by vital status and by thirds of muscular strength. Differences in covariates were tested using Student t tests, χ2 tests, and F tests. We used Cox proportional hazards regression to estimate hazard ratios, 95% confidence intervals, and mortality rates (deaths per 10000 person years of follow-up), according to exposure categories. We adjusted multivariable analyses for age in model 1, and in model 2 for age, physical activity (sedentary or active), smoking (current smoker or not), alcohol intake (≥5 drinks a week or not), body mass index, baseline medical conditions (presence or not of hypertension, diabetes, or hypercholesterolaemia), and family history of cardiovascular disease. Additional analyses were done after further adjustment for cardiorespiratory fitness (model 3), entered as a continuous variable. A total of 2548 (29.1%) participants reported a family history of cardiovascular disease. As none of the participants reported a family history of cancer, therefore we omitted this variable from the model.

Muscular strength and functional aerobic capacity decline steadily with age.4243 To determine whether the association between muscular strength and mortality differed by age, we examined the association in younger men (<60 years) and older men (≥60 years). To determine whether the association between muscular strength and mortality varied by body weight, we examined the association within defined categories of body mass index for normal weight (18.5-24.9 kg/m2) and overweight (≥25 kg/m2). Finally, we examined the combined effects of muscular strength and cardiorespiratory fitness on all cause mortality. For this analysis we created six categories for combinations of strength and fitness on the basis of thirds of muscular strength and we dichotomised these in to fit groups and unfit groups. We compared the effect of each combination of strength and fitness status (low strength, unfit; middle strength, unfit; high strength, unfit; low strength, fit; middle strength, fit; high strength, fit) with the referent group (low strength, unfit). Cumulative hazard plots grouped by exposure suggested no appreciable violations of the proportional hazards assumption. Excluding deaths that occurred during the first two years of follow-up did not materially change the results. We calculated two sided P values and we considered those <0.05 as significant. Analyses were done using SAS statistical software, version 9.1.

Results

During an average follow-up of 18.9 years and 165251 person years of observation 503 (5.7%) deaths occurred: 145 (28.8%) from cardiovascular disease and 199 (39.6%) from cancer. Table 1​1 shows the baseline characteristics of the participants on the basis of vital status and thirds of muscular strength. Muscular strength, as well as exercise duration and maximal metabolic equivalents, were significantly higher in survivors than in decedents. A direct gradient of treadmill test duration across thirds of muscular strength was observed. Age, body mass index, blood pressure, and levels of total cholesterol, triglycerides, and glucose were higher in decedents and in those with lower levels of muscular strength.

Table 2​2 shows age adjusted death rates and hazard ratios (model 1) for death from all causes, cardiovascular disease, and cancer; hazard ratios after further adjustment for smoking, alcohol intake, body mass index, baseline medical conditions, and family history of cardiovascular disease (model 2); and hazard ratios after additionally adjusting for cardiorespiratory fitness (model 3). All cause mortality rates were 1.50 (38.9/25.9) and 1.46 (38.9/26.6) times greater for those in the lowest third of muscular strength than for those in the middle and upper thirds of muscular strength, respectively. The corresponding differences for death from cardiovascular disease were 1.83 (12.1/6.6) and 1.59 (12.1/7.6) times greater, and for death from cancer were 1.45 (6.1/4.2) and 1.24 (6.1/4.9) times greater. The age adjusted results of model 1 showed a notably declining risk of death from all causes, cardiovascular disease, and cancer across incremental thirds of muscular strength (all P<0.01 for linear trend). After the additional adjustments (model 2), risks of death from all causes and cancer remained progressively lower with higher levels of muscular strength (both P<0.05 for linear trend), whereas the association between muscular strength and death from cardiovascular disease was attenuated (P=0.08 for linear trend). The association between muscular strength and risks of death from all causes or cancer remained significant after further adjustment for cardiorespiratory fitness (both P<0.05 for linear trend), whereas the association between muscular strength and risk of death from cardiovascular disease was no longer statistically significant once the analysis was further adjusted for cardiorespiratory fitness (P=0.23 for linear trend).

Rates and hazard ratios for mortality in men from all causes, cardiovascular disease, and cancer by thirds of muscular strength

Figure 1​1 shows the association between muscular strength and death from all causes or cancer in men aged <60 years (n=8397) and ≥60 years (n=383) and figure 2​2 shows the association in men of normal weight (n=3890, 44%) and overweight men (n=4782, 56%). Strong statistical evidence existed of an interaction between muscular strength and age in predicting death from all causes (χ2=10.46 (df=1), P=0.001) and cancer (χ2=6.49 (df=1), P=0.01) but weak statistical evidence of an interaction between muscular strength and body mass index in predicting death from all causes (χ2=1.71 (df=1), P=0.19) and cancer (χ2=1.62 (df=1), P=0.20). A significant inverse gradient was found for death from all causes and cancer across incremental thirds of muscular strength within each age group (both P<0.05 for linear trend). Across age strata, those in the lowest third of muscular strength had a 1.4-fold to 2.2-fold and 1.5-fold to 4.3-fold higher rates of mortality from all causes and cancer than those in the upper third of muscular strength. Muscular strength also was inversely associated with all cause mortality in men of normal weight and overweight men (both P<0.05 for linear trend) and with cancer mortality in overweight men (P=0.03 for linear trend; fig 2​2).). Across incremental thirds of muscular strength, age adjusted total death rates per 10000 person years were 32.9, 25.6, and 20.7 in men of normal weight (P=0.009 for linear trend) and 42.1, 26.2, and 33.8 in overweight men (P=0.046 for linear trend).

Fig 1Age adjusted death rates per 10000 person years from all causes and cancer by thirds of muscular strength and age groups

Fig 2Age adjusted death rates per 10000 person years by thirds of muscular strength and body mass index groups

Figure 3​3 shows the age adjusted death rates for all causes across thirds of muscular strength and cardiorespiratory fitness categories (unfit and fit), which resulted in six groups for strength and cardiorespiratory fitness. The death rate in the unfit men with the lowest muscular strength was the highest among the six combination groups. The adjusted death rate per 10000 person years was inversely related to muscular strength within the fit cardiorespiratory fitness group (P=0.008 for linear trend), and inversely related to cardiorespiratory fitness within the lowest third (P=0.003) and middle third (P=0.03) of muscular strength.

Fig 3Number of men and age adjusted all cause death rates per 10000 person years according to thirds of muscular strength and cardiorespiratory fitness categories. Adjusted death rate was inversely related to muscular strength within...

Discussion

Muscular strength was significantly and inversely associated with risk of death from all causes and cancer after controlling for potential confounders, including cardiorespiratory fitness. The inverse association was consistent in strata of age (<60 and ≥60 years) and body mass index (18.5-24.9 and ≥25 kg/m2). Muscular strength was significantly and inversely associated with risk of death from cardiovascular disease after controlling for age, yet the association was attenuated once other potential confounders were entered in the model and was not significant after further adjustment for cardiorespiratory fitness. The analysis on the combined effects of muscular strength and cardiorespiratory fitness with all cause mortality showed that the age adjusted death rate in men with high levels of both muscular strength and cardiorespiratory fitness was 60% lower (P<0.001) than the death rate in the group of unfit men with the lowest levels of muscular strength. These results highlight the importance of having at least moderate levels of both muscular strength and cardiorespiratory fitness to reduce risk of death from all causes and cancer in this population of men.

We investigated the association between standardised measures of upper and lower body muscular strength and disease specific risk of mortality in a large cohort of men with extensive follow-up. Muscular strength and cardiorespiratory fitness were moderately correlated (age adjusted partial r=0.33), suggesting that the association between muscular strength and risk of death from cancer works at least partially through different mechanisms than those associated with the protective effects of cardiorespiratory fitness. That the association between muscular strength and risk of death from cardiovascular disease was not significantly independent of cardiorespiratory fitness highlights the key role of cardiorespiratory fitness in the development of cardiovascular disease in men; however, their combined effects cannot be easily disentangled in an observational study. In this cohort the number of deaths from cardiovascular disease was lower than that from cancer (145 and 199, respectively). This may have reduced the statistical power to detect a significant independent association between muscular strength and risk of death from cardiovascular disease.

Apart from our preliminary analyses in the aerobics centre longitudinal study,28 only one study has assessed the association between muscular strength and all cause mortality after adjusting for cardiorespiratory fitness and age, smoking status, and body mass index and found that handgrip strength and upper body strength (push-ups) were not significantly associated with risk of death from all causes.18 A significant inverse association between muscular strength (measured by handgrip strength) and risk of mortality has been reported in several other studies.1516171920212223242527 The main limitation of this test is that the measurement is highly influenced by the grip span of the dynamometer and hand size.444546 None of these previous studies standardised the grip span or assessed a second muscle group. Furthermore, hand grip uses a relatively small muscle group and is not well correlated with measures of overall muscular strength as determined by measurements of strength using large muscle groups.47 Assessing additional muscle groups may provide a better overall index of muscular strength, especially when measured in large muscle groups. Moreover, cardiorespiratory fitness was not measured in these studies, and we know that cardiorespiratory fitness is strongly associated with morbidity and mortality.78910111213373839

The apparent protective effect of muscular strength against risk of death might be due to muscular strength in itself, to muscle fibre type or configuration, or as a consequence of regular physical exercise, specifically resistance exercise. Muscle fibre type and configuration has a genetic component and influences strength, yet it is clear that resistance type physical activities are major determinants of muscular strength.548 We have previously reported a strong and positive association between the frequency of self reported resistance exercise and maximal muscular strength in men enrolled in the aerobics centre longitudinal study—that is, the higher the participation in resistance exercise the higher the muscular strength.32 This observation suggests that the measurements of muscular strength obtained in the present study provide an adequate representation of the resistance exercise habits in our cohort. Results from intervention studies indicate that resistance training enhances muscular strength and endurance, muscle mass, functional capacity, daily physical activity, risk profile for cardiovascular disease, and quality of life.5 These factors are well known predictors of higher risk of mortality. The benefits of resistance training are evident in men and women, young adults, and older people, in overweight and obese adults as well as in people of normal weight, and in people with or without disability, or with cardiovascular disease.5 We observed an inverse association between muscular strength and risk of death from all causes and cancer in older men (≥60 years) and younger men (<60 years). This suggests that older men may also benefit from having higher levels of muscular strength. In older men, functional performance becomes more directly dependent on strength, as these men also experience age associated declines in muscle mass and consequently in muscular strength.

The observed association between muscular strength and risk of death from all causes or cancer was also independent of body weight. We showed an inverse association between muscular strength and risk of death from all causes and cancer in overweight and obese men, as well as between muscular strength and risk of all cause mortality in those of normal body weight. Body mass index may have a different meaning in those who have greater muscular strength. For example, leg press strength for a man weighing 60 kg would be expected to be lower than that for a man weighing 90 kg. That is why we included body mass index in the multivariate analyses. Thus we not only controlled for the effect of weight but also height, which might have influenced the torque or force production. A high body mass index can result from a greater amount of fat or muscle. Yet in epidemiological studies most of the people with a higher body mass index also had higher fat levels.

Given that the prevalence of overweight and obesity exceeds 66% in the United States49 and that overweight and obese people are at a substantially higher risk of disability50 and death,5152 these results have important implications for public health. Exercise recommendations to prevent or treat obesity have focused mainly on aerobic activities, yet resistance exercise is an important complement for weight control, mainly as a result of the increases in metabolically active muscle mass.2 Under most circumstances, and especially during physical inactivity, resting energy expenditure is the largest component of total energy expenditure. The energy expenditure related to muscle metabolism is the only component of resting energy expenditure that might vary considerably.2 The resting metabolic requirements of splanchnic tissues, brain, and skin vary little under normal conditions, mainly because of their relatively constant mass and protein turnover rates. In contrast, large variations in muscle mass are possible, and the rate of muscle protein turnover (synthesis and breakdown) may vary as well. The synthesis and breakdown of muscle protein are principally responsible for the energy expenditure of resting muscle. In theory, every 10 kg difference in lean mass translates to a difference in energy expenditure of about 100 kcal daily, assuming a constant rate of protein turnover.2 A difference in energy expenditure of about 100 kcal daily translates to about 4.7 kg of fat mass yearly. Over a long period the maintenance of a large muscle mass and consequent muscle protein turnover can contribute to the prevention of obesity. Therefore it is reasonable to presume that when sustained over time, resistance exercise training should help to prevent or revert increases in body fat.5

Strengths and limitations

The results of the present study should be interpreted with caution. Generalisation of the findings may only apply to well educated white men of middle to upper socioeconomic status. Values for blood pressure and cholesterol levels, body weight, and cardiorespiratory fitness from participants in the aerobics centre longitudinal study were similar to those reported in two population based studies in North America.8 Moreover, there is no reason to believe that the benefits of muscular strength would be different in other ethnic or socioeconomic groups. Because of the limited sample of women, who contributed relatively few deaths to the main study, we were unable to perform a meaningful parallel analysis on women. Therefore women were not included in this substudy. No detailed information about drug use or diet was available, which may have biased the results through residual confounding. It seems unlikely, however, that these factors would account for all of the observed association between muscular strength and mortality. That none of the participants reported a family history of cancer might be a limitation of the main study owing to self selection bias. In fact, only 1.16% of men in the entire cohort of the aerobics centre longitudinal study reported a family history of cancer. Future studies should include such information whenever possible.

A major strength of this study was the inclusion of objective and standardised maximal tests for muscular strength (upper and lower body) and cardiorespiratory fitness using highly reliable measurement protocols in a large cohort of men with extensive follow-up. Undetected subclinical disease is always a concern in any observational study, but it is less likely to have occurred in our cohort because of the comprehensive physical examination and the clinical assessment completed by each participant. Moreover, participants were healthy enough to achieve at least 85% of aged predicted maximal heart rate during the treadmill test.

Conclusions

Muscular strength was independently associated with risk of death from all causes and cancer in men. These findings are valid for men of normal weight, those who are overweight, and younger or older men, and are valid even after adjusting for several potential confounders, including cardiorespiratory fitness. Muscular strength seems to add to the protective effect of cardiorespiratory fitness against the risk of death in men. Whether the association between muscular strength and risk of death from cardiovascular disease is independent of typical confounders as well as of cardiorespiratory fitness warrants further investigation.

Prospective studies among diverse populations and among women are needed to examine the independent and combined associations of muscular strength and cardiorespiratory fitness with disease specific mortality. It might be possible to reduce all cause mortality among men by promoting regular resistance training involving the major muscle groups of the upper and lower body two or three days a week.5 Resistance training should be a complement to rather than a replacement for aerobic exercise. The recommendation for moderate to vigorous physical activity and resistance training are supported by the current research owing to the reduction in risk of death from all causes and cancer associated with increased cardiorespiratory fitness or muscular strength.3456

What is already known on this topic

Cardiorespiratory fitness provides strong and independent prognostic information about the overall risk of illness and death

Most prospective studies examining the association between muscular strength and death have had limitations

What this study adds

Muscular strength in major muscle groups is independently associated with death from all causes and cancer in men aged 20-82

These findings are valid for those who are of normal weight or overweight, younger or older, and even after adjusting for several potential confounders, including cardiorespiratory fitness

Muscular strength seems to add to the protective effect of cardiorespiratory fitness against the risk of death in men

Notes

We thank the doctors and technicians at the Cooper Clinic who collected the baseline data and staff at the Cooper Institute for data entry and data management.

Contributors: All authors were involved in the concept and design of the study, the analysis and interpretation of data, drafting the manuscript, and critical revision of the manuscript for important intellectual content. They are guarantors. XS and SNB acquired the data. JRR, XS, FL, JRM, AWJJr, and SNB did the statistical analysis.

Funding: The aerobics centre longitudinal study was supported by the National Institutes of Health (grants AG06945, HL62508); the Spanish Ministry of Education (AP2003-2128, EX-2007-1124); the Margit and Folke Pehrzon Foundation; the European Union, in the framework of the public health programme (ALPHA project, 2006120); the American Heart Association predoctoral fellowship; and the American College of Sports Medicine Paffenbarger-Blair fund for epidemiological research on physical activity.

Competing interests: None declared.

Ethical approval: This study was approved by the Cooper Institute institutional review board.

33. Pickering TG, Hall JE, Appel LJ, Falkner BE, Graves J, Hill MN, et al. Recommendations for blood pressure measurement in humans and experimental animals: part 1: blood pressure measurement in humans: a statement for professionals from the Subcommittee of Professional and Public Education of the American Heart Association Council on High Blood Pressure Research. Circulation 2005;111:697-716. [PubMed]