Article Figures & Data

Figures

IDR-1002 treatment reduced the CFU burden in mice infected with P. aeruginosa PA103. Mice were given water or IDR-1002 at 24 h prior to infection (−24 h), given saline or P. aeruginosa PA103 at 0 h, and then euthanized, and samples were processed at 18 h. (A) CFU counts from BALF. (B) Leukocyte counts in BALF. (C) Distribution of leukocytes in BALF, represented as means and standard errors of the mean (SEM). (D) Percent weight loss. (E and F) Health scores at 3 h or 18 h postinfection. The data represent 9 or 10 mice per condition from a combination of two experiments and were analyzed using an unpaired two-tailed t test for the CFU (A), a one-way ANOVA and Tukey’s multiple-comparison test for the total leukocytes (B), and a Kruskal-Wallis test with Dunn’s multiple-comparison test (C to F). *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001; ns, not significant.

IDR-1002 treatment reduced cytokines and chemokines in BALF and serum that were induced by P. aeruginosa PA103. Mice were given water or IDR-1002 at −24 h, given saline or P. aeruginosa PA103 at 0 h, and then euthanized, and samples were processed at 18 h. ELISAs were performed for MCP-1 in BALF (A) and serum (B), KC in BALF (C) and serum (D), IL-6 in BALF (E) and serum (F), and TNF-α in BALF (G) and serum (H). The data represent mean ± SEM for 9 or 10 mice per condition from a combination of two experiments and were analyzed using one-way ANOVA and Tukey’s multiple-comparison test. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ns, not significant.

Zero-order protein-protein interaction network of DE genes that are associated with leukocyte migration and their interacting partners in the lungs of PA103-infected versus negative-control mice. The red nodes were upregulated and the green nodes were downregulated in the PA103 group. A darker color indicates a stronger change. Chemokines are circled in blue.

Zero-order protein-protein interaction network of DE genes that are associated with ECM organization and their interacting partners in the lungs of PA103-infected versus negative-control mice. The red nodes were upregulated and the green nodes were downregulated in the PA103 group. A darker color indicates a stronger change. The genes for matrix metalloproteinase MMP-9 and components of collagen are circled in blue.

Zero-order protein-protein interaction network of DE genes in the blood of PA103-infected versus negative-control mice. The red nodes were upregulated and the green nodes were downregulated in the PA103 group. A darker color indicates a stronger change. Several key genes involved in the immune response to infection are circled in blue.

Zero-order protein-protein interaction network of DE genes in the lungs of IDR-1002 treatment versus PA103-infected mice. The red nodes were upregulated and the green nodes were downregulated in the treatment group. A darker color indicates a stronger change. Several key genes involved in the immune response to infection are circled in blue.