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Sugar-immobilized metal nanoparticle, method for measuring sugar-protein interaction using the same and method for recovering protein from sugar-protein interactant

Sugar-immobilized metal nanoparticle, method for measuring sugar-protein interaction using the same and method for recovering protein from sugar-protein interactant

外国特許コード

F110005671

整理番号

V350P001WO

掲載日

2011年9月9日

出願国

アメリカ合衆国

出願番号

92087806

公報番号

20090240032

公報番号

8067393

出願日

平成18年5月26日(2006.5.26)

公報発行日

平成21年9月24日(2009.9.24)

公報発行日

平成23年11月29日(2011.11.29)

国際出願番号

JP2006310592

国際公開番号

WO2006126689

国際出願日

平成18年5月26日(2006.5.26)

国際公開日

平成18年11月30日(2006.11.30)

優先権データ

特願2005-154550
(2005.5.26)
JP

2006WO-JP310592
(2006.5.26)
WO

発明の名称
（英語）

Sugar-immobilized metal nanoparticle, method for measuring sugar-protein interaction using the same and method for recovering protein from sugar-protein interactant

発明の概要（英語）

(US8067393)It is intended to provide a stable novel sugar-immobilized metal nanoparticle capable of easily immobilizing a sugar chain, a method for measuring sugar-protein interaction easily and at a low cost using the same without labeling, and a method for simply recovering a protein from a sugar-protein interactant. A maltose-immobilized gold nanoparticle was obtained by binding a ligand complex, in which maltose and a linker compound had been bound to each other, to a gold nanoparticle. By adding this maltose-immobilized gold nanoparticle to a dilution series of concanavalin A, a sugar-protein interactant of maltose and ConA was formed, and red-purple color derived from a colloidal solution of maltose-immobilized gold nanoparticle disappeared. That is, sugar-protein interaction could be confirmed by visual observation without labeling.

特許請求の範囲（英語）

[claim1]1. A sugar-immobilized metal nanoparticle, which comprises: a ligand complex of a structure including a linker compound comprising thioctic acid and m-phenylenediamine at a molar ratio of 1:1, and a sugar having a reducing end, the linker compound including a hydrocarbon structure which includes: a hydrocarbon inducing chain with an aromatic amino group at an end and a carbon-nitrogen bond in a backbone; and a sulfur atom, the sugar being bound to the linker compound via the amino group; and gold bound to the ligand complex, wherein the sugar-immobilized metal nanoparticle is obtained by mixing the ligand complex with a solution of a gold nanoparticle prepared by mixing sodium tetrachloroaurate (III) with sodium borohydride, wherein the sugar-immobilized metal nanoparticle is stable, wherein a solution of the sugar-immobilized metal nanoparticle contains sodium tetrachloroaurate (III) at a concentration of 0.5 mM to 4 mM, sodium borohydride at a concentration that is 3 to 10 times a molar concentration of gold ion, and the ligand complex at a concentration of 10 mu M to 1000 mu M, and wherein the sugar-immobilized metal nanoparticle forms a sugar-protein interactant through interaction of a sugar and a protein, and wherein the sugar-immobilized metal nanoparticle forms a colloid solution when mixed with an aqueous solution.[claim2]2. A method for measuring sugar-protein interaction, comprising the step of forming a sugar-protein interactant through interaction of sugar and protein by mixing a solution containing the sugar-immobilized metal nanoparticle of claim 1 with a protein that recognizes the sugar situated at the end of the sugar-immobilized metal nanoparticle.[claim3]3. A method for recovering a protein from a sugar-protein interactant, comprising the steps of: forming a sugar-protein interactant through interaction of sugar and protein by mixing a solution containing the sugar-immobilized metal nanoparticle of claim 1 with a protein that recognizes the sugar situated at the end of the sugar-immobilized metal nanoparticle; and adjusting a pH of an admixture solution of the sugar-protein interactant and water to 5 or less.[claim4]4. A method for recovering a protein from a sugar-protein interactant, comprising the steps of: forming a sugar-protein interactant through interaction of sugar and protein by mixing a solution containing the sugar-immobilized metal nanoparticle of claim 1 with a protein that recognizes the sugar situated at the end of the sugar-immobilized metal nanoparticle; and mixing the sugar-protein interactant with a sugar that is recognizable by the protein.