[Objectives]The aim of the present study was to explore the effects of dietary enzymatically treated Artemisia annua(EA) on the main digestive organs weights,nutrient apparent availability,and small intestinal mucosal disaccharidase activities in heat-stressed broilers. [Methods]A total of one hundred and forty-four 21 day-old healthy male Arbor Acres(AA) broilers were randomly allocated into three groups,with six replicated pens per treatment and 8 broilers per pen. Broilers in the control(CON) and heat-stressed(HS) groups were fed the basal diet while ones in HS-EA group were fed the basal diet with 1 000 mg·kg-1 EA. And the broilers in CON group were housed at 22℃ while the other ones were reared at 34℃ for 8 h(09:00 to 17:00) and 22℃ for the rest time. The period of experiment was 21 d. Heat treatment lasted for 20 consecutive days. [Results]Compared with CON group,heat treatment significantly decreased(P<0.05) nutrient apparent availability of ether extract(EE),crude protein(CP),and organic matter(OM),the activities of maltase and sucrose in jejunum and ileum,and ileal lactase activities in broilers. Compared with HS group,dietary EA supplementation significantly increased(P<0.05) apparent availability(EE,CP and OM),the activities of maltase and sucrose in jejunum and ileum,and ileal lactase activities. [Conclusions]Dietary 1 000 mg·kg-1 EA supplementation could alleviate compromised nutrient apparent availability and intestinal mucosal disaccharidase activities of broilers induced by heat stress.