This assay has high sensitivity and excellent specificity for detection of Mouse GAS6. No significant cross-reactivity or interference between Mouse GAS6 and analogues was observed.

Assay Type

Sandwich

Detection Range

31.25pg/ml-2000pg/ml

Sensitivity

17.086 pg/ml

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV% is less than 8%Three samples of known concentration were tested twenty times on one plate to assess.Inter-assay Precision (Precision between assays): CV% is less than 10%Three samples of known concentration were tested in twenty assays to assess.

Detection Wavelength

450 nm

Sample Volume

50-100ul

Protein Biological Process 1

Cardiovascular

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Product Note

Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Other Notes

Small volumes of GAS6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GAS6 purchase

MBS939560 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the growth arrest-specific 6 (GAS6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25pg/ml-2000pg/ml in biological research samples containing GAS6, with an estimated sensitivity of 17.086 pg/ml. The ELISA analytical biochemical technique of the MBS939560 kit is based on GAS6 antibody-GAS6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GAS6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GAS6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for gas-6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any gas-6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for gas-6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of gas-6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.