Description:
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4, a cysteine protease required for autophagy, cleaves the C-terminal part of MAP1LC3 to form the activated molecule LC3-I. LC3-I is subsequently conjugated with phosphatidylethanolamine at the C-terminal glycine to form LC3-II, a marker for autophagy via its capacity to bind to autophagosomes.

Applications:Suitable for use in Western Blot, Immunohistochemistry, ELISA

Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type

Isotype

Clone

Grade

Pab

IgG

Affinity Purified

Size

Storage

Shipping

Source

Host

200ul

-20°C

Blue Ice

Human

Rabbit

Concentration:

As reported

Immunogen:

ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264~293 amino acids from the central region of human APG4B.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Specificity:

Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.