Human AP17(Apelin 17) ELISA Kit

Intended use

This ELISA kit can be applied to the in vitro quantitative determination of Human AP17 concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Human AP17. During the reaction, Human AP17 in the sample or standard competes with a fixed amount of Human AP17 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human AP17. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human AP17 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay type

Competitive

Format

96T

Assay time

2.5h

Reactivity

Human

Detection Method

Colormetric

Detection Range

0.16—10 ng/mL

Sensitivity

0.10 ng/mL

Sample Volume

50μL

Sample Type

Serum, plasma and other biological fluids

Specificity

This kit recognizes Human AP17 in samples. Nosignificant cross-reactivity or interference was observed.

Linearity

Samples were spiked with high concentrations of Human ACTH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Serum (n=5)

EDTA plasma (n=5)

Cell culture media (n=5)

1:2

Range (%)

99-113

84-97

93-104

Average (%)

105

91

99

1:4

Range (%)

84-95

94-108

106

Average (%)

89

100

106

1:8

Range (%)

90-101

87-100

94-107

Average (%)

95

93

101

1:16

Range (%)

87-100

90-101

97-112

Average (%)

94

96

104

Kit components & Storage

The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.

Item

Specifications

Storage

Micro ELISA Plate(Dismountable)

8 wells ×12 strips

-20℃, 6 months

Reference Standard

2 vials

Concentrated Biotinylated Detection Ab (100×)

1 vial, 120 μL

Concentrated HRP Conjugate (100×)

1 vial, 120 μL

-20℃(shading light), 6 months

Reference Standard & Sample Diluent

1 vial, 20 mL

4℃, 6 months

Biotinylated Detection Ab Diluent

1 vial, 14 mL

HRP Conjugate Diluent

1 vial, 14 mL

Concentrated Wash Buffer (25×)

1 vial, 30 mL

Substrate Reagent

1 vial, 10 mL

4℃(shading light)

Stop Solution

1 vial, 10 mL

4℃

Plate Sealer

5 pieces

Product Description

1 copy

Certificate of Analysis

1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.