Inflammation is involved in many fields including cosmetology, drug discovery (including RNA-based vaccines), and diagnostic index development, as well as being linked to almost every disease such as HIV, cancers, or neurodegenerative diseases. For this reason, analysis of the inflammasome is a major concern. Immunoassays are a classic and affordable method to quantify the corresponding proteins secreted by cells during inflammation. Nevertheless, there are well-known drawbacks and limitations raising key questions about the sensitivity, reproducibility and even the universality of the results. We can’t all use mass spectrometry, but fortunately, ELISA tests and multiplexing have evolved. Let’s discover how much.

Immune checkpoint molecules play an important role in T cell functionality after TCR/MHC signalling. Blockade of two B7/CD28 family checkpoint molecules, CTLA-4 and PD-1, have already demonstrated excellent efficacy in increasing T cell responses to a variety of tumours. Identification of novel target and new checkpoint blockade remains a key element in Drug Discovery (See “Drug discovery: Immunotherapy checkpoint research for new Cancer treatments“). In this post, let’s take a look at the “Quantibody® Human Immune Checkpoint Molecule Array 1” for the detection of 10 Human Immune Checkpoint biomarkers.

Cell-Based ELISA tests now enable precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based assays is made.

In living organisms, ExtraCellular Matrix (ECM) builds up a complex and dynamic network of molecules, playing a major role in structural support, adhesion, movement and cellular regulation. Major components of the ECM (Collagen, Proteoglycans, Elastin, Hyaluronan) are key elements studied in the Pharmaceutical and Cosmetology fields due to their strong involvement in tissue regeneration, skin repair, wound healing, ageing diseases, surgery, inflammation, and also tumour development.

Boost your ECM studies with these 5 kits!

To help you in monitoring this component, let’s take a look at five robust and easy-to-use, in vitro assays, with colorimetric read-outs for easier daily use in your research.