The Punching Appratus for Cover Slips,

which make it possible to subculture adherent cultured cells

WITHOUT the cell dispersion

Report

A novel passage method for adherent cultured cells: CS Divider

This development was motivated by a request for a cell-cultured coverslip sheet to be divided into uniform individual pieces. The CS Divider reduces and simplify your routine work for cell dispersion. The following explanatory introduction of this product is based on data from a doctor after using CS Divider.

< Background and Goal >

The passage of adherent cultured cells normally requires the cell dispersion procedure occurring on tripsin / EDTA, etc. The first passage may however not be built easily due to potential proliferactive arrest and cellular death during the cell dispersion. Therefore, we suggest a novel method for cell subculturing based on a simple sheet division without cell cleavages.

< Experimental and Results >

HeLa cells were cultivated overnight on a coverslip sheet (Cell Desk LF2, Sumitomo), followed by its division into 56 hexagonal pieces of individual coverslip using CS Divider (Fig. 1). Afterwards the cell culturing procedure was continued (Fig.2), and we confirmed that the multiplication of the cells 2d and 7d after the cell subculturing (Fig.3). The experiment was double-checked.