... Hi,
I would look into SCnorm package ([https://github.com/rhondabacher/SCnorm][1]).
Paper link: [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5473255/][2]
Important point:
> To evaluate the extent to which biases introduced during normalization affect the identification of DE genes, we appl ...

... This is a good question for the beginners who are starting with the NGS analysis.
> In common short read sequencing, the DNA insert (original molecule to
> be sequenced) is downstream from the read primer, meaning that the 5'
> adapters will not appear in the sequenced read. But, if the fr ...

... Did you look into the interactive mode? This is what you have done in the past when you login into HPC and log to one node. ....You can use interactive mode to login to the node and run commands there. ...