Purpose: The purpose of this study was to identify the relationship between health behaviors and musculoskeletal disease in adults. Methods: The data of 7,421 adults applied in this study were collected from health behaviors (smoking, drinking, exercise, weight, sleeping, stress) and related with musculoskeletal disease form 2009 Korea National Health and Nutrition Examination Survey. Date were collected through self-report questionnaires from January to December, 2009. Data were analyzed using chi-square test, Multivariate logistic regression with SPSS/WIN 17.0. Results: The prevalence rate of musculoskeletal disease was 30.6%. In multivariate analyses, the sex, age, BMI, stress and education were statistically strongly associated with most of musculoskeletal disease among adults. Conclusion: The results of this study suggest that musculoskeletal disease prevention and nursing intervention programs should be necessarily established and continuously managed in order to treat with the musculoskeletal disease.