The Aperio Area Quantification FL Algorithm separates a fluorescence stained tissue image of up to 3 channels, and quantifies the intensity and area of each fluorescent dye. It also identifies and measures colocalization between the different channels, providing the user with a full and accurate picture of the biomarker expression pattern in the tissue area.

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Specify dyes precisely

To get the best results for your specific samples, tell the algorithm which dyes were used on the slide that was scanned. Exclude non-specific fluorescence and highly fluorescent artefacts from analysis with user-defined threshold settings.

Get in-depth results

View results as numeric data, including Pearson’s Coefficient and Overlap Coefficient values for each pair of dyes, and plot histograms of the analysis results. A variety of mark-up images can be generated, including colocalization masks for different combinations of dyes.

Customize settings

Get the most from your fluorescence samples, with precise and accurate image analysis. Aperio Area Quantification FL Algorithm offers highly-tunable inputs to get the most relevant data from your slides, including fine-tuning of channel colors, and the ability to select which dyes to colocalize.