RE: [Histonet] fixation of fresh frozen section

From:

"Lewis, Sarah"

I would love more information on this subject. Thanks in advance!!
Sarah E. Lewis
Histotechnician
Childrens Research
Center for Gene Therapy
700 Childrens Dr Rm WA3112
Columbus OH 43205
(614)-722-2204
LewisS@ccri.net
-----Original Message-----
From: Edmondson David (RBV) NHS Christie Tr
[mailto:David.Edmondson@christie-tr.nwest.nhs.uk]
Sent: Wednesday, August 03, 2005 12:37 PM
To: Bruijntjes, J.P.
Cc: Histonet (E-mail 2)
Subject: RE: [Histonet] fixation of fresh frozen section
Hi
I recently heard a presentation where they spoke of Periodate-Lysine-Paraformaldehyde in the context of Frozen sections. The coagulant fixatives do not alter the proteins chemistry so on might suggest no loss of antigenicity, the "Gold Standard". But the morphology of PLP is much better. It is fifteen and more years since I used it myself and maybe a search would be a quicker way to find the concentrations. I ahve an email contact if you need to ask him.
David
Christie
Manchester UK
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
Bruijntjes, J.P.
Sent: 03 August 2005 14:39
To: histonet@pathology.swmed.edu
Subject: [Histonet] fixation of fresh frozen section
Hi all
When I do immunocytochemistry on fresh frozen section I fix most, if not
all slides with acetone. Sometimes I use cold methanol because the
datasheet tells me to do so. Is anyone of you who prefer other
fixatives?
Joost Bruijntjes
TNO Quality of Life
Zeist
Holland
This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mai