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Recombinant Human CCL27/CTACK (Catalog # 376‑CT) chemo­attracts the BaF3 mouse pro‑B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Preservative

No Preservative

Purity

Protein A or G purified from hybridoma culture supernatant

Reconstitution Instructions

Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL27/CTACK Antibody (124315)

ALP

C-C motif chemokine 27

CCL27

chemokine (C-C motif) ligand 27

CTACK

CTACKSmall-inducible cytokine A27

CTAK

cutaneous T-cell attracting chemokine

Cutaneous T-cell-attracting chemokine

ESKINE

IL-11 Ralpha-locus chemokine

ILC

ILCCC chemokine ILC

PESKY

SCYA27IL-11 R-alpha-locus chemokine

skinkine

small inducible cytokine subfamily A (Cys-Cys), member 27

Background

CCL27, also known as CTACK (cutaneous T cell-attracting chemokine), ALP, ILC, and ESkine, is a member of the CC family of chemokines (1). Mature human CCL27 is an 88 amino acid (aa) protein that shares 57% aa sequence identity with mouse and rat CCL27 (2). It shares 11%‑35% aa sequence identity with other human CC chemokines. An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration (3). CCL27 is constitutively expressed by keratinocytes and is up‑regulated by inflammatory stimuli and in wounded skin (4‑7). CCL27 binds the chemokine receptor CCR10, glycosaminoglycans in the extracellular matrix, sulfated tyrosine residues on PSGL-1, and determinants on the surface of fibroblasts and endothelial cells (5, 7‑9). CCL27 cooperates with CCL17/TARC in inducing the migration of cutaneous lymphocyte antigen (CLA) positive memory T cells to the skin during inflammation (4, 6, 10‑12). Endothelial cell-bound CCL27 can mediate the adhesion of those cells to CLA+ T cells (6). CCL27 also induces the migration of keratinocyte precursors from bone marrow to the skin, thereby promoting wound healing (7). In humans, serum CCL27 levels are elevated and correlate with disease severity in atopic dermatitis, psoriasis vulgaris, and mycosis fungoides (13‑15).

Kunkel, L. and E.C. Butcher (2002) Immunity 16:1.

Ishikawa-Mochizuki, I. et al. (1999) FEBS Lett. 460:544.

Gortz, A. et al. (2002) J. Immunol. 169:1387.

Morales, J. et al. (1999) Proc. Natl. Acad. Sci. 96:14470.

Homey, B. et al. (2000) J. Immunol. 164:3465.

Homey, B. et al. (2002) Nat. Med. 8:157.

Inokuma, D. et al. (2006) Stem Cells 24:2810.

Jarmin, D. et al. (2000) J. Immunol. 164:3460.

Hirata, T. et al. (2004) J. Biol. Chem. 279:51775.

Vestergaard, C. et al. (2004) Exp. Dermatol. 13:551.

Reiss, Y. et al. (2001) J. Exp. Med. 194:1541.

Soler, D. et al. (2003) Blood 101:1677.

Kakinuma, T. et al. (2003) J. Allergy Clin. Immunol. 111:592.

Hijnen, D. et al. (2004) J. Allergy Clin. Immunol. 113:334.

Fujita, Y. et al. (2006) Clin. Cancer Res. 12:2670.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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