RE: [Histonet] fibrin gel to paraffin

From:

"Patsy Ruegg"

Betsy,
I would wrap them in paper (perm curl paper) and process as usual on a
tissue processor, I use a shorter than usual schedule for animal tissue and
things like this, 20 min in each change of alcohols, xylene and paraffins.
Embed on edge to get a cross section of all the layers.
Patsy
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari,
Betsy
Sent: Thursday, August 21, 2008 5:44 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] fibrin gel to paraffin
Hi,
I have some "outgrowths" that are in a modified fibrin gel. They vary in
size from.1 to 1mm thick. The gel is porous and optically clear for the
most part. They have been fixed in 4% paraformaldehyde. They were done
in well plates or chamber slides.
The researchers want them processed for paraffin. I have no experience
with this technique and would be grateful for any suggestions.
Thank you.
Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave.
MC 1-283
Houston, TX 77030
832-355-6524
832-355-6812 (fax)
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