I am currently following a protocol that uses a buffer termed RIPA
lysis buffer. The buffer is currently being used in the preparation of
conceptus homogenates which are subsequently assayed for DNA content and
protein concentrations. I have a suspicion that our recipe for this buffer
is incorrect. Does anyone know if this is a commonly used buffer? If so,
what is its composition? Specifically, the problem appears to be the Triton-
X 100 concentration of the buffer which interfers dramatically with our DNA
fluorometric assay. Any comments or suggestions are welcomed.
Thank You,
Mike L. Green