Autoclaving MOPS buffer

10x MOPS buffers do go yellow if you autoclave them. I used to but don't now
since I have no problems with my Northens. I presume than the formaldehye you
add when making up the Northern buffer would kill or inhibit any contaminating
RNase.
Another thing, store your 10X MOPS buffer in the dark or else they go yellow
again.
C-TJ Chan Tel. +44 71 242 9789 Ext 2351
Dept of Molecular Genetics, Fax. +44 71 831 0488
Institute of Child Health, email cchan at hgmp.mrc.ac.uk
30 Guilford Street,
London WC1N 1EH
United Kingdom