ab40938 staining RAB14 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. HeLa cells were infected with C. trachomatis serovar L2 (MOI 5) for 10 hours. The cells were fixed in paraformaldehyde, permeabilised with saponin and then blocked using 0.2% BSA for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/100 for 1 hour at 25°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to FITC (green) used at a 1/200 dilution. Microtubules were evinced by a mouse monoclonal anti-ß-tubulin (1/200) followed by donkey anti-mouse Cy5-labeled antibodies (1/700) (red). Endogenous Rab14 was recruited to the chlamydial inclusion (blue asterisk). Bacteria were labeled with Hoechst 0.1 mg/ml (blue). ab40938 was useful to detect for the first time the recruitment of an endogenous RAB to the chlamydial inclusion.

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