None more black (or none more white) is a real concern when you’re talking about imaging western blots. If you only want to verify the presence of a protein, you can stop reading here, because even an overexposed image will tell you that there is some protein present in your sample. But an overexposed image of a western blot is essentially useless when you’re … Continue Reading

The following information is an updated version of a method for using ImageJ to analyze western blots from a now-deprecated older page.

If you’re looking for a more comprehensive workflow option for your western blot analyses, please visit my tutorial on using Image Studio Lite, a free software package from LI-COR Biosciences. The Image Studio Lite software can be downloaded for free from www.licor.com/islite. In addition to the functionality described below for ImageJ, Image Studio Lite provides additional … Continue Reading