﻿﻿﻿In this work, we demonstrate a definitive molecular diagnosis platform (DhITACT-TR) for rapid and accurate detection of MERS coronavirus. DhITACT-TR offers a robust diagnosis of target RNA pathogens in pseudo-serum specimens with minimum liquid handling as compared to conventional PCR analysis. In addition, a definitive diagnostic result can be achieved within 30 min by an optical detection.