Human skin punch biopsy from patients after skin grafting. Sample was taken at the site of re-epithelialization 7 days after surgery.

Control

normal skin tissue (skin grafting)

Skin biopsy samples from healthy control subjects after breast or abdomen reduction surgery.

wound healing study 2 (ex vivo; DMSO) / normal skin tissue (ex vivo)

Relative Expression (log2-ratio):-4.2436724
Number of Samples:3 / 3

Experimental

wound healing study 2 (ex vivo; DMSO)

Ex vivo skin samples obtained from healthy donors following reduction surgery of abdomen, and incubated in culture medium containing DMSO for 4 days. To make sure that mainly epidermis was present in the samples, as much dermal tissue as possible was removed by dissection. Skin was sliced into 1x10 mm slices and incubated in keratinocyte medium for four days with 1:1000 fold dilution of DMSO. The cultivation was performed in serum-free keratinocyte medium supplemented with transferrin, hEGF (0.15 ng/mL), 0.5 mg/mL hydrocortisone, gentamicin, amphotericin B, and epinephrine but without insulin.

Control

normal skin tissue (ex vivo)

Normal skin samples obtained from healthy donors following reduction surgery of abdomen. To make sure that mainly epidermis was present in the samples, as much dermal tissue as possible was removed by dissection.

Skin punch biopsy from patients that underwent split-thickness skin grafting. Sample was taken from the wound site 7 days after grafting. Patients received negative-pressure wound therapy (NPWT) immediately after grafting .