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Clinical Informatics Use Case Clinician B sends patient for baseline imaging to radiologist Patient A goes to an imaging center, and gets an MR image for her breast cancer (Level 3, image file) Radiologist report is sent back to the clinician (Level 2) Patient gives sample to the pathologist (Level 0) Pathologist sends sample to genomic lab service C (Level 0) Lab service runs labs and sends the results back to the pathologist (FSH, IHC, GxP, SNP, et al.) (Level 3) Pathologists collates result from labs and his/her analysis of the tissue and send the pathology report back to the clinician (Level 2) Physician uses pathology report and radiology report to make diagnosis, and discusses treatment options with patient (Level 1) (These activities generally in practice occur as an iterative loop, and often non-concurrently.)

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Translational Research Use Case (1/3) Clinical Researcher D creates a hypothesis based on bench research, clinical research, and population research (this is from survey of the literature and interactions with colleagues. ) (Level 1) D writes a clinical protocol for validation of a biomarker as predictive of tumor shrinkage in the context of treatment and submits it to the sponsor (Level 3, PDF) The trial is approved and initiates Patients are recruited directly (patients themselves) or indirectly (via clinician) for screening Participants are screened on the basis of eligibility Screening data and sample are supplied to trialist by patient for enrolment evaluation (Level 3 (ex. CRF)) Trialist sends sample to the basic scientist for biomarker screening (Level 0) Basic scientist sends the result of biomarker screening to the trialist (Level 3)

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Translational Research Use Case (2/3) Based on eligibly criteria and biomarker results, the trialist sends the enrolment notification to patient (Level 2) Clinical trialist refers patient to radiologist for imaging (Level 1) Radiologist acquires MR images (Level 3) Radiologist analyzes images and creates image annotations (Level 3) and passes along the trialist Clinical trialist collects tissue samples (Level 0) and sends to the pathologist (for diagnosis and for distribution to the basic researcher (biomarker assessment) (Level 0) Basic researcher runs the biomarker assays and sends analysis results (Level 3) to trialist The pathologist sends staging and grading to the trialist (Level 2) (This is repeated throughout the study) The trialist starts the treatment, and the cycle of image collection, tissue collection and molecular data collection continue throughout the trial, and all information is sent back to the trialist throughout the duration of the trial (Level 3)

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Translational Research Use Case (3/3) The trial closes and ends, and all the data is provided to the statistician, (Level 3) Statistician communicates the clinical significance of and evidence for biomarker response prediction (Level 3) The statistician, approved by the clinical trialist, sends the report to the sponsor (Level 2) Trialist, basic scientist and statistician write a scientific paper reporting the results (Level 1) Data goes back to the sponsor (Level 3)