#*Note: According to the [http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=2648393 original TSS paper] and qualitative experience ([[Josh Michener|JM]]), this step is completely optional and may actually reduce transformation efficiency.

#*Note: According to the [http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=2648393 original TSS paper] and qualitative experience ([[Josh Michener|JM]]), this step is completely optional and may actually reduce transformation efficiency.

Save the rest of the transformants in liquid culture at 4 °C. If nothing appears on your plate, you can spin this down, resuspend in enough medium to spread on one plate and plate it all. This way you will find even small numbers of transformants.