Lead accumulation in the marine environment is the result of anthropogenic use. The precipitation characteristic of lead elevates the risk of this metal exposure to the benthic communities. The study aimed to access the influence of acidic and basic environment on the toxicity of lead to the benthic organism, the whiteleg shrimp L. vannamei through acute toxicity test. The median lethal concentration was evaluated using probit analysis, and the univariate analysis of variance was used to determine which factors more likely influenced the mortalities of the test animal. At both pH 6.5 and 8.5, the higher Pb concentration resulted higher mortality of the test animals, and at 1000 ppm, all the shrimps were dead even at earliest observation. The LC50 values at pH 6.5 and 8.5 were 216.57 ppm and 259.84 ppm respectively in which Pb metal pollution at both pH is still practically non-toxic to the whiteleg shrimps, L. vannamei. Furthermore, based on the univariate ANOVA, both pH as well as the interaction of Pb and both pH do not significantly cause the mortality of the test animal as F-stat < F-table. The only factor that significantly caused the mortality of the shrimps was Pb concentration as F-stat > F-table. Therefore, there is no synergistic toxicity effects of Pb in both pH 6.5 and 8.5 to the whiteleg shrimps, L. vannamei.