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Localization Microscopy ofDNA Replication Foci

In the later stages of S phase of cells undergoing mitosis, stretches of DNA which are harder to replicate remain as replication active foci. These can be visualized by the incorporation of EdU which can be specifically linked to AF488. A comparison of images of nuclei from U2OS cells acquired by widefield and localization microscopy is shown (Scale bar: 2µm).