Based on the assumption that genes with related function from different organisms
show high sequence similarity, degenerate primers can be designed from sequences of
homologues genes. Genefisher 2 is an interactive web-based program for designing degenerate primers.
The procedure leads to isolation of genes in a target organism using multiple alignments
of related genes from different organisms. The term "gene fishing"
refers to the technique where PCR is used to isolate a postulated but unknown target
sequence from a pool of DNA.

BatCons2 is a tool for Backtranslation of aminoacid-sequences to nucleicacid-sequences and for ambiguos consensus sequence calculation based on a given alignment.