Prostate cancer is the number one cancer in males in Africa, both in terms of incidence and mortality, accounting for 40,000 (13%) male cancers and 28,000 (11.3%) male cancer-associated deaths. In the developed world, the probability of being diagnosed with cancer is more than twice as high as in developing countries. In developing countries, most cancer victims are diagnosed at late stage, with incurable tumors, pointing to the need for education schemes and better detection programs. This study assessed knowledge , attitudes and perceptions of prostate cancer among male staff of the University of Nigeria. This cross-sectional descriptive study was carried out with 655 male staff who agreed to participate and were recruited on giving oral consent. A self-administered questionnaire, written in English was used. The mean percentage knowledge score was 71.2%. Some 57.8% of respondents had a high knowledge level of prostate cancer. The mean percentage attitude score was 69.9%. More than half (60.8%, n = 397) of respondents had a positive attitude towards prostate cancer screening and treatment. The mean percentage perception score was 60.0%. More than half (53.9%, n = 351) of respondents had a negative perception of prostate cancer screening and treatments. The staff of the University of Nigeria have appreciable knowledge and a positive attitude with regard to prostate cancer. A significant proportion of staff however, exhibited poor knowledge and negative attitudes and perceptions of prostate cancer screening and treatment.