Scientists determined to find PSA source

Sixteen months after the discovery of Psa in New Zealand kiwifruit, Otago biochemsits are confident they will discover the source of the outbreak.

Associate Professor Russell Poulter, Professor Iain Lamont and Dr Margi Butler, all from the University of Otago's Department of Biochemistry, have been working with the kiwifruit industry since the Psa (Pseudomonas syringae pv. Actinidiae) bacterium was first detected in a Te Puke orchard in November 2010.

From the outset, the source of the bacterium has been a key question. There are three possible options: Chile, China or Europe, namely Italy.

Initially, the technology was not available within New Zealand to carry out sequencing the whole genetic code of the New Zealand Psa strain. But, the arrival of new advanced genomics technology into New Zealand mid last year - through the governmentfunded initiative, New Zealand Genomics Limited (NZGL) - allowed the Otago biochemists to action this urgent task. The results showed the New Zealand strain was significantly different from the Italian strain and, therefore, Italy could not have been the source. Critically, it also put to bed any whispers that New Zealand may have been the source of historical Italian outbreaks.

Through a process of elimination, Chinese pollen appears to be the likely source of New Zealand's outbreak.

The Otago biochemists have been able to source some Chinese Psa samples for sequencing and the results indicate that China is the original source of both the Italian and New Zealand outbreaks. More recently, Psa has been confirmed in Chile and work has begun on sequencing these strains. Preliminary data suggests that the Chilean strain also originates from China.

Associate Professor Poulter says the question of whether the New Zealand outbreak may have come via Chile will soon be answered, by using the NZGL sequencing process and comparing the results with the information they have gathered to date.

"Having provided the tools, through sequencing, to recognise the different strains, we now want to determine the pathway by which the Psa came into the New Zealand. It's unlikely to be on someone's boots or via quarantined channels. And we know the particular strain did not originate from Europe. We need to know the pathway, so we can help other countries - and ourselves - avoid similar mistakes in future."

Two years ago, the Government committed $40m over 10 years to establish NZGL - a collaborative infrastructure of genetic sequencing technology and expertise, involving Auckland, Massey and Otago Universities. It was NZGL's multi-million dollar Illumina HiSeq sequencing service that the Otago biochemists used to determine the Psa genome and gave the kiwifruit industry the answers it needed.

Dr Poulter says the kiwifruit Psa scenario has highlighted to him how critical it is to have the technology within New Zealand.

"New Zealand is a major agricultural and horticultural producer. It's what we are. It's how we earn a living. If something goes wrong, are we going to put a sample on the courier and send it to another country for sequencing? No. We need to be able to respond, prioritise and act quickly. If NZGL wasn't here, we would never have been able to sequence the bacterium and answer the industry's questions."

NZGL chief executive Tony Lough says the infrastructure is doing precisely what it was set up for: to give New Zealand scientists access to the technology needed to undertake advanced genomics research specific to New Zealand. This is work that could not previously be undertaken in New Zealand. While, in theory, you could contract advanced genomics

analysis to labs overseas, the reality was that this was expensive and often took too long. Why would a facility in another country prioritise work from elsewhere?

"The way in which the Otago scientists used NZGL, and the speed with which NZGL was able to process their samples, demonstrates exactly why it's so critical to have this advanced technology available within New Zealand."

Meanwhile, what next for the kiwifruit industry? The Ministry of Science and Innovation, the kiwifruit industry and grower/packhouse company Seeka Kiwifruit Industries have collectively committed $330,000 over three years to continue working with the Otago researchers.

Associate Professor Poulter says the team will concentrate on the effective use of antibiotics for the successful treatment of Psa in New Zealand.

"The kiwifruit industry will survive, but it may take some time to determine a cost effective treatment. The priority is to sustaining the industry in the meantime. Part of that is containing the bacterium - and determining how it came to be here."