RUVBL1 possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. It is the component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex.

The information below lists the predicted species and target name associated to the peptide antigen. Please note, all available target reference numbers to the antigen sequence are presented, including unreviewed and protein isoforms.To search for antibodies by species, please visit Aviva’s Species Reactivity Page. To search Aviva’s catalog of antibodies by sequence, please visit Aviva’s Antibody Blast Tool.

Description of Target: RUVBL1 possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. It is the component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex.

Aviva Systems Biology’s RUVBL1 antibody (ARP32349_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

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Positive control - K562 cells/ negative control - knockdown of gene in Drosophila cells using dsRNA

Please include your detailed WB Procedure/Protocol here:

Samples ran in 12% SDS-polyacrylamide gel at 100-130V. Proteins were transferred from gel to membrane in 1h 30min at 300mA. Blocking - 1h at room temperature in 5% milk. Incubation with RUVBL1 antibody - either overnight at 4°C or 1h at room temperature. Dilution 1:200 in 5% milk. Washing - 3x 10 minutes in PBST buffer (0.05%). Incubation with secondary antibody - 1h at room temperature. Dilution of goat-anti-rabbit - HRP antibody 1:1000 in 5% milk. Washing - 3x 10 minutes in PBST (0.05%). Developed by Clarity Western ECL substrate (Biorad) and exposed to film.
comment to the image:
line 1 - K562 positive control
line 2 - S2 cells control
line 3 - S2 cells with silenced RUVBL1
RUVBL1 should show band around 50 kDa, however in S2 cells there wasn't any band about this size even with high concentration of antibody and loading of 30 μg of protein sample. In K562 leukemic cells there was a band above 55 kDa (MW marker PageRuler Plus Prestained Protein Ladder, Thermo Scientific).