ABSTRACTVascular Early Response Gene (Verge) is an immediate early gene (IEG) that is up-regulated in endothelial cells in response to a number of stressors, including ischemic stroke. Endothelial cell lines that stably express Verge show enhanced permeability. Increased Verge expression has also been associated with blood brain barrier breakdown. In this study we investigated the role of Verge in ischemic injury induced by middle cerebral artery occlusion (MCAO) in both Verge knockout (KO) and wild type (WT) mice. Verge KO mice had significantly less cerebral edema formation after MCAO compared to WT mice. However, stroke outcome (infarct size and neurological deficit scores) evaluated at either 24 or 72 hours after stroke showed no differences between the two genotypes. Verge deletion leads to decreased edema formation after ischemia; however acute stroke outcomes were unchanged.

Mentions:
Absolute water content in the ipsilateral (stroke) hemisphere of both KO and WT mice was significantly increased compared with that of the contralateral hemisphere after stroke (Figure 2A). Verge KO mice had significantly less water content in the ipsilateral hemisphere compared to WT mice. The absolute water content in the contralateral hemisphere of KO mice was also less than that in WT mice although this difference was not significant. We also calculated the relative water content, comparing each genotype to its contralateral (non-stroke) hemisphere, which showed no difference between KO and WT mice (Figure 2B).

Mentions:
Absolute water content in the ipsilateral (stroke) hemisphere of both KO and WT mice was significantly increased compared with that of the contralateral hemisphere after stroke (Figure 2A). Verge KO mice had significantly less water content in the ipsilateral hemisphere compared to WT mice. The absolute water content in the contralateral hemisphere of KO mice was also less than that in WT mice although this difference was not significant. We also calculated the relative water content, comparing each genotype to its contralateral (non-stroke) hemisphere, which showed no difference between KO and WT mice (Figure 2B).

ABSTRACTVascular Early Response Gene (Verge) is an immediate early gene (IEG) that is up-regulated in endothelial cells in response to a number of stressors, including ischemic stroke. Endothelial cell lines that stably express Verge show enhanced permeability. Increased Verge expression has also been associated with blood brain barrier breakdown. In this study we investigated the role of Verge in ischemic injury induced by middle cerebral artery occlusion (MCAO) in both Verge knockout (KO) and wild type (WT) mice. Verge KO mice had significantly less cerebral edema formation after MCAO compared to WT mice. However, stroke outcome (infarct size and neurological deficit scores) evaluated at either 24 or 72 hours after stroke showed no differences between the two genotypes. Verge deletion leads to decreased edema formation after ischemia; however acute stroke outcomes were unchanged.