Gosh Darn it!You wanted tuse LB? All protocols I have ever done use SOC which is richer. Your transformation is going to the weeds my friend. Or not.You might have decreased a little bit the efficiency, but YPD is quite the rich medium anyway, so I guess they should recover nicely. Do not worry.

Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)