Via a magic mirror, Twilight Sparkle travels into an alternate universe in order to recover a crown that was stolen from the Crystal Empire. Upon her arrival she is horrified to learn that she has turned into a human.

A new dark force threatens Ponyville, and the Mane 6 – Twilight Sparkle, Applejack, Rainbow Dash, Pinkie Pie, Fluttershy and Rarity – embark on an unforgettable journey beyond Equestria where they meet new friends and exciting challenges on a quest to use the magic of friendship and save their home.

"Get your muzzle out of those books and make some friends!" That's what Princess Celestia tells Twilight Sparkle. She may be the smartest unicorn in Equestria, but Twilight Sparkle gets an "incomplete" in friendship. There's more to life than learning magic, after all -- so she goes to Ponyville on a mission to make friends. There she meets five special ponies who take her on exciting adventures and teach her the most powerful magic of all ... the magic of friendship!