The initials IVF stands for in vitro fertilization,with “in vitro” being Latin
for “in glass”- a reference to the glass test tubes used in the laboratory. This
is where the phrase ’test tube baby’, often used in the media, stems from.

In vitro fertilization, in simple language, is growing, collecting and combining
eggs with spermin the laboratory to create embyos to be transferred to a woman’s
womb.

IVF treatment –in summary– involves administration of fertility drugs to
stimulate your ovaries to produce a number of eggs and then, using a minor
surgical procedure,we collect your eggs and place them in a culture dish( rather
than a test tube) with prepared sperm for fertilization.

One or two of the resulting embryos can then be transferred to your womb, and
any suitable embryos not used in this cycle can be frozen for you to use in
future.

It is generally used in couples who have failed to conceive after at least one
year of trying who also have one or more of the following:

1. Blocked fallopian tubes or pelvic adhesions with distorted pelvic anatomy.
Women that have had tubal ligation and are considering tubal reversal surgery as
well as men that are considering vasectomy reversal surgery might also consider
IVF.

4. Advanced female age - over about 38 years of age. In vitro fertilization and
advanced maternal age is discussed in detail on the female age page.

5. Reduced ovarian reserve, which means lower quantity (and sometimes quantity)
of eggs. A day 2/3 FSH and estradiol test, antral follicle counts and AMH
hormone levels are often done as screening tests for egg quantity. Reduced egg
quantity and quality is usually treated with either IVF, or with IVF with egg
donation.

6. Severe endometriosis

7. Unexplained infertility when inseminations have failed. Unexplained
infertility means standard fertility tests have not found the cause of the
fertility issue.

Intracytoplasmic Sperm Injection

ICSI stands for Intracytoplasmic Sperm injection.The procedure follows on from
egg collection and involves injecting a single sperm into the centre of each
mature egg,to help fertilization to occur.

We might recommend ICSI when:

• The number of sperm is low.

• Motility of sperm poor.

• High number of abnormal sperms.

• Surgically collected sperms.

• In previous unexplained failures to achieve fertilization in conventional
IVF,or when very few eggs have been fertilized following IVF.

Blastocyst Culture :

This term describes the process when the embryos grow for longer in the
laboratory,and undergo critical developmental chages before being returned to
the womb.

This period of extended culture helps our experts select the most competent
embryo(s) for transfer to your womb,and can help to indicate which embryos have
the best growth potential.Therefore,blastocyst culture maximizes the chance of
you achieving viable pregnancy.

By the time an embryo has reached the blastocyst stage,its alresdy undergone
several cell divisions and achieved its first cell differentiation into two
distinct cell types.

Although this period of extended culture doesn’t necessarily improve the quality
of the embryos,or gurantee that they reach the blastocyst stage,we have
impressive success rates for this treatment.

Who needs Blastocyst culture?

You are likely to benefit from this treatment if you have large number of
embryos.That’s because not all embryos have the ability to form a blastocyst,and
the number of embryos progressing each day may decrease. This advanced technique
allows the most competent embryo(s) to be selected for the transfer on fifth day
following egg collection. The treatment cycle is exactly same as of IVF,except
that the embryos will be cultured longer in laboratory.This simply means the day
of your embryo transfer will be five days after egg collection.

Embryo Freezing

Embryos are often frozen during an IVF cycle when fresh embryos are transferred
and additional embryos created in that cycle ,but not transferred at the time
,are frozen.Sometimes,due to medical reasons all embryos are kept frozen.They
can be stored for your future use for upto 5 years.(acc to ICMR guidelines.)

Who needs Embryo freezing?

• This may be an option for woman who are facing sugeries or cancer therapies
that may affect your ability to produce healthy eggs in future,either with sperm
of your partner or donor semen.

• Surplus good quality embryos for future use.

• Embryo freezing for medical reasons like to avoid hyperstimulation,in lieu
with poor endometrium or if any problem with patient’s health

Frozen Embryo Transfer:

It means replacing thawed frozen embryos in the womb at the optimum time for
implantation.Oral medications and/or minimal amount of injections required for
endometrial lining preparation.This helps the woman avoid undergoing injections
for growth of eggs again.

Sperm freezing:

Sperm freezing and bankingis a form of fertility preservation,intended to keep
open your options for having child in future.

Who needs sperm freezing?

Impending surgery or treatment that may damage your fertility is the most common
reason for freezing,e.g vasectomy and treatment of cancer. In cases of low sperm
count we do back up semen freezing for short term. Renewal of sperm freezing is
done every 12months in cases of long term freezing and every 3mths in case of
short term freezing after proper approval from the patient.

The surgical retrieval of sperm may enable the 1-2% of patients who have no
sperms in the semen.(a condition known as azoospermia).This may be due to:
.Failure of the sperm passage to develop(congenital absence of the vas deferens)
A blockage of the sperm transport tubules( rete,epididymis or vas deferens) A
previous vasectomy operation. Insufficient sperm production in testis due to
various diseases,congenital problem and X-ray treatments.

A. PESA stands for Percutaneous epididymal sperm aspiration ,this procedure is
usually done under sedation with pain relief but can also be done under General
anesthesia. Sperms are directly extracted from epididymis(duct above the testis)
by using a fine needle which is passed through the skin of scrotum ,small amount
of fluid is obtained and examined for sperms.If sperms are found,they are used
for ICSI/or kept frozen.

B. TESA stands for testicular sperm aspiration,this procedure is usually done
under GA. Sperm is extracted directly from the testicles by passing a fine
needle directly into the testicle,small amount of tissue is extracted and
examined and preserved for sperm.