Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XVII Written by GROWTH AND ACTIVITY OF CELLULASE-AMYLASE ENZYME PENICILLIUM NALGIOVENSE AND ASPERGILLUS TAMARII MOLDS ISOLATED FROM COW RUMEN FLUID

Cow rumen fluid is a fluid rich in cellulolytic microbes that play a role to help digest food that contains high crude fiber. Two species of moulds have been isolated from the cellulolytic rumen fluid of local cattle and have identified as Penicillium nalgiovense and Aspergillus tamarii. Cellulolytic Index both of Penicillium nalgiovense and Aspergillus tamarii were 2.33 and 1.24 respectively. The purpose of this study is to characterize their growth and activities of cellulase and amylase. Research include: 1) growth of mould 2) macroscopic description, and 3) activities of cellulase and amylase enzyme using the DNS (3,5-dinitrosalicylic acid) method. Based on the growth curve is known that the logarithmic phase of growth of Penicillium nalgiovense peak occurred on day 6 with a population of 9.696 x 103 cfu cells, whereas Aspergillustamarii logarithmic phase, occurred on day 5 with a population of 4.65 x 102 cfu cells. Cell morphological characters of P. nalgiovense as conidium colour milky white, and spore powdery, in the early growth of Penicillium nalgiovense white colonies after a while the colonies get old is also white and conidiophores with smooth stipe. As macroscopic A.tamarii is yellow-brownish, at the beginning of growth colonies colour is a bright yellow growth after several days, color were changed to dark brownish yellow. This mold has a conidial head yellow-brown,not columnar and have ornaments that are not clear. Cellulase enzyme activity in Penicillium nalgiovense amounted to 2.420 units / ml, and amylase enzyme activity of 2.146 units / ml. Aspergillustamariii to be used for degradation of fiber for feed.