In article <199602130318.VAA22448 at borcim.wustl.edu> brett at BORCIM.WUSTL.EDU (brett) writes:
>>hopkinsc at lincoln.ac.nz (Hopkins, Charlotte) wrote:
>>>Can anyone help me please. I spilt 0.5x TBE buffer containing Ethidium
>>>Bromide (200mg in 2.5 litres) on myself the other day. The jeans went into
>>>the wash for a couple of cycles as soon as they could. Does anyone know if
>> This problem came up a couple of months ago who posted a similar mishap,
>>and the best solution was .... toss the jeans. A means t=
>>o remove ethidium bromide from solution is to mix with activated charcoal ...
>>the mutagen is bound to the carbon and the solution ca=
>>I also contaminated an article of clothing once, in my case a brand new
>t-shirt from my vacation. I wasn't about to part with it, so I scrubbed it,
>and used a mild bleach solution, recalling the use of hypochlorite in
(omitted stuff here and there)
I would think that the ethidium bromide would be removed by a wash in
alcohol (e.g. ethanol, get the cheapest brand). This is rather like the
effect of butanol extractions of CsCl bands: The ethidium partitions in
the alcohol phase. After that, a thorough washing and line-dry in the
sun should do the trick.
Daniel Kim
just my opinion . . . no I don't have any data on it. :)