CXCR2 and CXCL2 expression in mouse colon tissues.

<p><i>CXCR2</i> (<b>A</b>) and <i>CXCL1</i>, <i>CXCL2</i>, <i>CXCL3</i>, and <i>CXCL5</i> (<b>B</b>) mRNA expression in the colons of the two groups sacrificed at the indicated times was assessed by quantitative RT-PCR. Each value represents mean ± SD (n = 7 mice/group). *p<0.05, **p<0.01 versus control mice or day 0 mice. CXCL2<sup>+</sup> (<b>C</b>, <b>D</b>) and CXCR2<sup>+</sup> (<b>E</b>) cells were detected in the mouse colon tissues by immunohistochemical analysis. The colons were removed from the AOM and DSS-treated mice at the indicated times. Immunohistochemical analysis was performed using anti-CXCL2 and anti-CXCR2 antibodies, as described in the Materials and Methods. The cell numbers per field were counted in 5 randomly selected visual fields at 400× magnification. Representative results of 7 mice are shown in (<b>C</b>). Values in (<b>D</b>) and (<b>E</b>) represent mean ± SD for 7 mice in each group. *p<0.05, **p<0.01 versus day 0 mice. (<b>F</b>) Neutrophils expressing CXCR2 were detected by double-color immunofluorescence analyses using anti-Ly6G (red) and anti-CXCR2 (green) antibodies, as described in the Materials and Methods. The fluorescent images are digitally merged in the far right image. Representative results of 7 mice are shown. Original magnification, 630×. (<b>G</b>) The number of migratory Neutrophils in response to varying CXCL2 concentrations was counted in >10 randomly selected visual fields at 400× magnification. Values are mean ± SD of 3 independent experiments, expressed as fold increases over the control cells. **p<0.01 versus untreated cells. (<b>H</b>) Results of quantitative RT-PCR of <i>IL-1α</i> mRNA expression are shown. Each value represents mean ± SD (n = 7 mice/group). *p<0.05, **p<0.01 versus control mice or day 0 mice.</p>