Bioprocess engineering research papers

Additional Indications for Approved Drugs. Proteins from Recombinant Microorganisms Extensive research on eukaryotic gene expression in bacteria, yeasts, plants, insects, and mammals has resulted in many options for producing proteins in recombinant hosts.

In spite of the numerous options, most of the products manufactured today are made either in recombinant E. No other microorganism is used to produce so large a number of products at high level.

Rapid progress in the development of E. The body of knowledge that has accumulated has facilitated the adaptation of this bacterium for foreign-protein expression. Sophisticated cloning vectors, tools for regulated gene expression, and knowledge about the process of protein secretion and the physiology of growth were available in E.

After translocation, the protein can accumulate within the periplasmic space or might be released to the surrounding medium. If the protein is secreted and accumulated within the cytoplasmic space, it normally aggregates into large inclusion bodies visible with a light microscope.

These must be isolated, solubilized, and folded to obtain an active molecule.

Isolation and solubilization are routine, but folding to an active form is difficult with present technology. Intracellular accumulation often has the additional disadvantage of producing a substance with an extra amino acid on the N terminus of the protein.

Several signal sequences are now available to drive the secretion of eukaryotic proteins across the bacterial cytoplasmic membrane. Occasionally, that results in the formation of properly folded, bioactive proteins.

More often, however, the secreted proteins also accumulate as aggregates in the periplasmic space; again, it is necessary to isolate, solubilize, and fold the proteins to their proper conformation.

The journal Bioprocess and Biosystems Engineering is an international peer-reviewed forum for discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. Bioprocess Engineering and Fermentation Technology Biochemical Engineering Bioprocess Technology Bioprocess Development Fermentation Biotechnology All research related to Bioprocess Engineering M. Current Issue - Journal of Bioprocessing and Biotechniques displays the articles that are accepted and meant for publication during that month.

Perhaps the most exciting application for secretion of proteins from E. For both intracellular and secreted eukaryotic proteins, proteolytic degradation in E. Several approaches have been taken to reduce undesirable proteolysis, including the expression of fusion proteins and the elimination of specific proteases by host-cell mutation.

The latter approach has been useful, but continued removal of proteases can be expected to affect general cellular metabolism adversely.

Biopharmaceuticals

Mistranslation has also been an occasional problem, but published technology now exists to minimize it. The cells grow and express rDNA proteins rapidly and in high quantities.

They also are easily modified genetically and generally require inexpensive growth media. However, the system is often limited by its inability to produce intact, properly folded proteins and by a limited ability to yield posttranslational modifications, such as glycosylation and specific proteolytic modification.

Inclusion Bodies High levels of protein synthesis have been obtained with several intracellular expression systems, particularly in E. High expression of a foreign protein in the cytoplasm of E. Isolation of inclusion bodies by centrifugation has become an important first step in the purification and recovery of recombinant proteins.

Extensive protein-chemistry studies have revealed substantial fundamental information on the mechanism of inclusion-body formation. Various solubilization agents have been defined strong chaotropes, detergents, and organic solvents for use in recovery of active proteins; the process requires unfolding the protein with strong denaturants and refolding to an active monomer.

Studies of the refolding of denatured proteins both in vitro and in vivo indicate that aggregates derive from specific partially folded intermediates and not from mature native or fully unfolded proteins Mitraki and King, Those discoveries focused attention on the properties of intermediates as distinct from native states and the factors interacting with them, such as the intracellular cytoplasmic environment, cofactors, and molecular chaperones.

Molecular chaperones were first identified as host proteins needed for phage morphogenesis and have recently been identified as heat-shock proteins Goloubinoff et al.

In a recent review Pelham,it was proposed that heat-shock proteins can act as molecular chaperones and prevent aggregation by binding to hydrophobic regions of partially unfolded polypeptide chains. On the basis of those fundamental discoveries, studies are under way to mimic the mechanics of mammalian protein synthesis compartmentation, interprotein interactions, and posttranslational modifications in bacteria.

With rational selection of the characteristics necessary for correct maturation, it might be possible to direct the fate of the intermediates toward the native conformation.Journal of Bioprocess Engineering and Biorefinery is a peer-reviewed multidisciplinary journal consolidates research activities in the fields of biotechnology, bioprocess engineering and biorefinery.

The journal publishes original (i) full research articles, (ii) rapid communications of important new scientific and technological findings, (iii) timely state-of-the-art reviews with author's photo and short .

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Bioscience & Engineering: An International Journal (BIOEJ) is a peer-reviewed, open access journal that addresses the impacts and challenges of Bioscience, Bioengineering and Applications.

His current main research focus is on bioprocess engineering and metabolic engineering.

and Fellow of ASABE. He has published over papers in refereed journals, books, and book chapters, and. Research outputs, collaborations and relationships for KAIST BioProcess Engineering Research Center (BPERC) published between 1 July - 30 June as tracked by the Nature Index.

The academic programs in the department of Paper and Bioprocess Engineering (PBE) emphasize fundamental engineering science and engineering skills pertaining to chemical engineering with specialization in the pulp, paper and allied industries, and the bioprocess and chemical industries.