TAS3 miR390-dependent loci in non-vascular land plants: Towards a comprehensive reconstruction of the gene evolutionary history https://t.co/SyL0jiZ15n
Trans-acting small interfering RNAs (ta-siRNAs) are transcribed from protein non-coding genomic loci and belong to a …

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Abstract

Trans-acting small interfering RNAs (ta-siRNAs) are transcribed from protein non-coding genomic loci and belong to a plant-specific class of endogenous small RNAs. These siRNAs have been found to regulate gene expression in most taxa including seed plants, gymnosperms, ferns and mosses.In this study,bioinformatic and experimental PCR-based approaches were used as tools to analyze TAS3 and TAS6 loci in transcriptomes and genomic DNAs from representatives of evolutionary distantBryophyta, Marchantiophyta and Anthocerotophyta.We revealed previously undiscovered TAS3 loci in classes Sphagnopsida and Anthocerotopsida, as well as TAS6 loci in Bryophyta classes Tetraphidiopsida, Polytrichopsida, Andreaeopsida and Takakiopsida. These data further unveil the evolutionary pathway of the miR390-dependent TAS3 loci in land plants. We also identified SGS3-coding sequences in charophytes and hypothesized that the appearance of TAS3-related sequences could take place at a very early step in evolutionary transition from charophyte algae to an earliest common ancestor of land plants.

Author Comment

This is a submission to PeerJ for review.

Supplemental Information

Nucleotide sequences of TAS3 loci in mosses of Sphagnum and Takakia genera

Alexey V. Troitsky conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the paper, approved the final draft.

Andrey G. Solovyev conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the paper, approved the final draft.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

New sequences described here are accessible via GenBank accession numbers MF682529 and MF682530.

Funding

The work of S. Morozov, T. Erokhina and A. Solovyev and was supported by the Russian Science Foundation (grant 17-14-01032). The work of I. Milyutina and A. Troitsky was supported by the Russian Foundation for Basic Research (grant 18-04-00574-а). The work of L. Ozerova was supported by the State Assignment of MBG RAS on the base of the Unique Scientific Installation “The Fund Greenhouse”. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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