Introduction

The kit is a sandwich enzyme immunoassay for the in vitro
quantitative measurement of SLC30A3 in human tissue homogenates or
other biological fluids.

Conform

Identification

Colorimetric

Positive

Composition

Traditional ELISA Kit

Ready-to-Use ELISA KIT

Conform

Pre-coated, ready to use 96-well strip plate 1

Pre-coated, ready to use 96-well strip plate 1

Plate sealer for 96 wells 2

Plate sealer for 96 wells 2

Standard 2

Standard 2

Diluents buffer 1×45mL

Standard Diluent 1×20mL

Detection Reagent A 1×120L

Detection Solution A 1×12mL

Detection Reagent B 1×120L

Detection Solution B 1×12mL

TMB Substrate 1×9mL

TMB Substrate 1×9mL

Stop Solution 1×6mL

Stop Solution 1×6mL

Wash Buffer (30concentrate) 1×20mL

Wash Buffer (30concentrate) 1×20mL

Instruction manual 1

Instruction manual 1

Test principle

The microtiter plate provided in this kit has been pre-coated with
an antibody specific to SLC30A3. Standards or samples are then
added to the appropriate microtiter plate wells with a
biotin-conjugated antibody preparation specific to SLC30A3. Next,
Avidin conjugated to Horseradish Peroxidase (HRP) is added to each
microplate well and incubated.

After TMB substrate solution is added, only those wells that
contain SLC30A3, biotin-conjugated antibody and enzyme-conjugated
Avidin will exhibit a change in color. The enzyme-substrate
reaction is terminated by the addition of sulphuric acid solution
and the color change is measured spectrophotometrically at a
wavelength of 450nm10nm.

The concentration of SLC30A3 in the samples is then determined by
comparing the O.D. of the samples to the standard curve.

Recovery

Matrices listed below were spiked with certain level of recombinant
SLC30A3 and the recovery rates were calculated by comparing the
measured value to the expected amount of SLC30A3 in samples.

Matrix

Recovery range (%)

Average(%)

serum(n=5)

80-102

91

EDTA plasma(n=5)

81-101

91

heparin plasma(n=5)

80-89

84

Linearity

The linearity of the kit was assayed by testing samples spiked with
appropriate concentration of SLC30A3 and their serial dilutions.
The results were demonstrated by the percentage of calculated
concentration to the expected.

Wuxi Donglin Sci & Tech Development Co.,Ltd., is a supplier of
biological reagents for life science, headquartered in the scenic
area beside the Taihu Lake. Founded in 1998, our company began
business as an OEM service provider of ELISA kits across the world.
In 2013, we launched our own brand DLdevelop. We specialize in
manufacturing ELISA kits and our products have been distributed to
the continents of Asia, Oceania, Africa, Europe and America.