We investigated the rat brain activity in acute seizures evoked by camphor tree essential oil or its main
constituent 1,8-cineole. The experiments were performed on adult male Wistar rats. The surgery and acute
electrophysiological recordings were performed under pentobarbital sodium anesthesia. Local field potentials
of cerebral and cerebellar parietal cortex were recorded before and 1 h after intraperitoneal (i.p.) camphor
essential oil or 1,8-cineole administration. The rats were intraperitoneally injected with camphor essential oil
(150-750 μl/kg b.w., in 1 ml of saline, i.p.) or 1,8-cineole (300-500 μl/kg b.w., in 1 ml of saline, i.p.). The
digitized electrocortical signals (sampling frequency: 256 Hz) were analyzed by the Fast Fourier Transform,
and were calculated Fractal dimension (FD) and Hurst exponent (H) also. Camphor essential oil (≥450 μl/kg
b.w.) and 1,8-cineole (≥300 μl/kg b.w.) induced seizure-like activity with single and multiple spiking of high
amplitudes in the parietal

electrocorticogram and occasional clonic limb convulsions, 3-15 min after their
administration. On the cerebrum, after camphor essential oil and 1,8-cineole administration, in epochs with
epileptic activity, there was high increase in total power and an increase in relative power in delta (0,1-4,0
Hz) frequency range, as well as a decrease in relative power in theta (4,1-8,0 Hz), alpha (8,1-15,0 Hz), beta
(15,1-32,0 Hz) and gamma (32,1-128,0 Hz) frequency ranges. It was confirmed the dominant presence of the
delta (0,1- 4,0 Hz) frequency range and the values of the relative power ranged from 47 to 87 %. On the
cerebellum, in epochs with epileptic activity, the relative power of the frequency ranges were behaving
differently than cerebrum, as well as after administration of 1,8-cineole compared to the camphor essential
oil. The differences between the cerebrum and cerebellum, as well as differences in the activities of the
camphor essential oil and 1,8-cineole, were more pronounced in the parameters of fractal analysis. On the
cerebrum, after camphor essential oil aministration, in epochs with epileptic activity, there was an increase in
the FD value compared to the control, and the decrease on the cerebellum. In contrast, after 1,8-cineole
administration there was a decrease in FD value on the cerebrum, and increase on the cerebellum. There was
a dose dependence of response after the camphor essential oil and 1,8-cineole administration. On the
cerebrum, increasing the dose of the camphor essential oil and 1,8-cineole, in epochs with epileptic activity,
correlated with an increase in relative power in delta (0,1 to 4,0 Hz) frequency range and a decrease in
relative power in theta (4,1-8,0 Hz), alpha (8,1-15,0 Hz), beta (15,1-32,0 Hz) and gamma (32,1-128,0 Hz)
frequency ranges. In contrast, on the cerebellum, increasing the dose of the camphor essential oil in epochs
with epileptic activity, correlated with a decrease in relative power in delta (0,1 to 4,0 Hz) and increase in
relative power in theta (4,1 to 8,0 Hz), alpha (8,1 to 15,0 Hz) and beta (15,1 to 32,0 Hz) frequency ranges. On
the cerebellum, increasing the dose of 1,8-cineole, in epochs with epileptic activity, correlated with an
increase in relative power in theta (4,1-8,0 Hz) and a decrease in relative power in gamma (32,1-128,0 Hz) frequency range...