Inhibitory effects of curcuminoids from Curcuma longa on matrix metalloproteinase-1 expression in keratinocytes and fibroblasts

Abstract

Because aging and inflammation mechanisms share common signaling pathways and mediators, natural products with anti-inflammatory effects are also good candidates as anti-aging agents. Bismethoxycurcumin, demethoxycurcumin, and curcumin are three curcuminoids that can be isolated from Curcuma longa and are well known for their anti-inflammatory and anti-cancer activities. In this study, we examined the anti-wrinkle effects of three curcuminoids from Curcuma longa in keratinocytes and fibroblasts to determine whether an anti-inflammatory agent could also be developed as an anti-aging treatment. The inhibitory effects of UV-B-induced upregulation of nuclear factor-kappaB (NF-κB) activity were detected by a reporter gene assay. Tumor necrosis factor (TNF-α) release was detected by reverse transcriptase-polymer chain reaction (RT-PCR), and matrix metalloprotease-1 (MMP-1) expression was determined by western blot and enzyme-linked immunosorbent assay (ELISA). UV-B induced TNF-α release through NF-κB activation in keratinocytes. Three curcuminoids inhibited the UV-B-induced TNF-αmRNA expression. Specifically, demethoxycurcumin and bisdemethoxycurcunim inhibited UV-B-induced NF-κB activation in HaCaT keratinocytes and reduced the expression of MMP-1 in both keratinocytes and fibroblasts. We found that curcuminoids have anti-wrinkle activity through the inhibition of UV-B induction in keratinocytes and TNF-α induction in fibroblasts. Our results suggest that wrinkle formation and inflammation have common signaling pathways.

Martini EM, Garrett N, Lindquist T, Isham GJ (2007) The boomers are coming: a total cost of care model of the impact of population aging on health care costs in the United States by Major Practice Category. Health Serv Res 42:201–218CrossRef