Tape slides face down onto film and
expose overnight in gel-cartridge.

Develop film. If tissue section is visible
on film and looks ok, incubate in emulsion for four days.
If tissue section either not visible or too dark on film,
readjust emulsion incubation time as appropriate.

Emulsion Dipping:

Bring water bath to 45 degrees C in darkroom. While water
bath is heating, remove emulsion aliquot from refrigerator
and allow to come to room temperature (takes about 40 minutes).

When water bath and emulsion reach temperature, make a 1:1
dilution of the emulsion with dH20. Invert once carefully. Be
careful not to agitate emulsion too much as this will increase
background and cause bubbles to form! Transfer emulsion
aliquot to water bath and allow it to reach 45 degrees
C (about 20 minutes).

Before dipping slides, test emulsion for bubbles
by dipping a blank slide. If slide has bubbles on it,
wait another ten minutes and dip another blank slide. Continue
until no or very few bubbles are present.

Dip relevant slides
as well as 2-3 blank slides in emulsion. Allow to
dry for 1-4 hrs. Check for dryness by checking blank
slides. When dry, place in light-tight box with dessicant “cigarette” and
store at 4 degrees C for appropriate amount of
time based on film reading. Be sure to store slides in a refrigerator
not containing radioactive material, and be sure not to agitate
slides as the emulsion is sensitive to shock.

Developing Slides:

Develop only one of the duplicate test slides
that should be positive to be sure slides are ready for development.
There should not be an excess or an absence of labeling.

In darkroom, allow boxes to come
to room temperature in boxes to avoid condensation which
causes image fading.