This reagent is not sold as sterile, but can be sterilized by filtration if necessary. To minimize loss of volume during filtration, first dilute to the final working concentration in the appropriate medium and then filter through a 0.22 u Millipore filter (or equivalent).

Presentation

250 ug purified Ig buffered in PBS + 0.02% NaN3

Preparation and Storage

Stable at 4 degree C. For long term storage, aliquot and freeze unused portions at -20 degree C in volumes appropriate for single usage. Avoid repeat freeze/thaw cycles.

Other Notes

Small volumes of anti-H-2Dd (private; H-2.m4) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

anti H-2Dd mAb is specific for cells expressing the H-2D antigen coded for by the d haplotype. The reaction pattern of this antibody with a panel of inbred and recombinant haplotypes demonstrates that the antibody detects a private determinant (H-2.4) of the H-2Dd antigen. This antibody can be used to quantitate or eliminate cells bearing H-2Dd (H-2.4) antigen from the appropriate strains of mice.

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Disclaimer

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