The primary objective is to establish the capabilities in obtaining high resolution 3-D and multiple component measurement. The research also emphasizes on applying these new techniques to advances our understanding of flow wall interactions with strong engineering and biomedical implications. Current projects focus on near wall flow structure manipulation via a moving surface with roughness element, developing measurement method to understand two-way coupling of unsteady flow and deforming vascular wall, hydrodynamic interactions of bacteria with the wall during biofilm formation and biofilm irrigation using micro-fluidics, and multi-scale investigation of planktonic flows during predation.