TaqMan® Array MicroRNA Cards

Coverage of the most relevant microRNA for human or rodent species, aligned with Sanger miRBase v20, with over 740 assays enabled across the sets of TaqMan® Array MicroRNA Cards (Cards A and B).

This product requires the use of Megaplex™ Primer Pools for miRNA reverse transcription. Optional Megaplex™ PreAmp Primers can be used for preamplification of limited samples prior to real-time PCR using the TaqMan® Array MicroRNA Card.

Applied Biosystems offers a variety of kits for the isolation and analysis of miRNA and other small RNAs, each is ideal for use in miRNA analysis because they enable quantitative recovery of small RNA (<200 nt) and maintenance of representative amounts of small RNA (eliminating experimental bias).

RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE
Extract total RNA or DNA from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) tissues. This kit enables the molecular analysis of archived tissue samples at both the genomic and gene expression levels. With this kit, nucleic acid is obtained that is suitable for RT-qPCR, mutation screening, and microarray analyses.

TaqMan® MicroRNA Reverse Transcription Kit
The TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for optimal performance of TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan® MicroRNA Assay to convert miRNA to cDNA.

Megaplex™ PreAmp Primers generate a comprehensive miRNA expression profile using as little as 1 ng of input total RNA. With content matched to specific Megaplex™ RT Primers and TaqMan® Array Human or Rodent MicroRNA Cards, cDNA prepared by reverse transcription can be preamplified using Megaplex™ PreAmp Primers and TaqMan® PreAmp Master Mix to uniformly amplify all miRNAs present in the original sample significantly increasing assay sensitivity.

Megaplex™ PreAmp Primers
For use when assay sensitivity is of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed miRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA.

For the final quantitation step, TaqMan® Universal Master Mix II is added to each sample and pipetted into the sample loading ports of a TaqMan® Array Card, greatly simplifying assay set-up and minimizing variability due to pipetting.

TaqMan® Array MicroRNA Cards
TaqMan® Array MicroRNA Cards are the ideal solution for profiling large numbers of miRNAs. This product requires the use of Megaplex™ Primer Pools for miRNA reverse transcription (Step 2). Optional Megaplex™PreAmp Primers can be used for preamplification (Step 3) of limited samples prior to real-time PCR using the TaqMan® Array MicroRNA Card (Step 4).

The real-time PCR is run on the Applied Biosystems® 7900HT Fast Real-Time PCR System.

The QuantStudio™ 7 Flex Real-Time PCR System
The QuantStudio™ 7 Flex Real-Time PCR System delivers the proven reliability, sensitivity, and accuracy of the ViiA™ 7 System in a new industrial design. The QuantStudio™ 7 Flex System has been optimized to enable the broadest range of quantitative PCR applications, with additional dyes, formats, and automation options.

For QuantStudio™ 12 Flex Real-Time PCR System
Combining flexible throughput capabilities with a streamlined workflow, the QuantStudio™ 12K Flex system takes you from targeted discovery through confirmation and screening, all on a single platform.

ViiA™ 7 Real-Time PCR System The ViiA™ 7 Real-Time PCR System puts high-productivity qPCR at your fingertips. With a streamlined workflow, one-button protocol feature, and intuitive software, the ViiA™ 7 Real-Time PCR System is faster to set up, easier to run, and more convenient to automate than any other qPCR platform. Using the ViiA™ 7 System with TaqMan® Array Micro Fluidic Cards provides an integrated workflow and faster results.

Integromics RealTime StatMiner® Software is a powerful, easy-to-use bioinformatics tool for quality control and differential expression analysis of real-time PCR data. The software easily imports raw data or Ct values from any Applied Biosystems® Real-Time PCR System and guides you though every step of analysis for accurate results in minutes.

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RealTime StatMiner® Software (optional)
RealTime StatMiner® Software from Integromics is a powerful, easy-to-use bioinformatics tool for quality control and differential expression analysis of real-time PCR data. With just a single click, the software imports raw data or Ct values from any of our real-time PCR instruments, and then guides you though every step of analysis, giving you accurate results in minutes.