[Histonet] IF in bone or cartilage paraffin sections

From:

pex

Hello, all.
I have done immunofluorescence in bone or cartilage paraffin sections
for several months. I use normal immunofluorescence protocol for
staining, but I have tried different methods for antigen retrieval, finally I
think that 0.1% trypsin (15min or 20min) for antigen retrieval in my
experiments is a little bit good.
In addition, I have already used different antibodies for
immunosatining. One of them is working well, positive expressions can be found in
cartilage, osteoblasts and osteocytes, but others express in cartilage
strongly, however they are very weak in bone( but they express stronger
in primary osteoblasts).I do not know the reasons!
Any suggestions will be useful for me!
Thank you!
Guofeng
Guofeng
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