Abstract : Assessment of serum liver enzyme and trace element levels was carried out in a total of hundred (100) subjects comprising seventy (70) sickle cell disease patients attending Madani learning hospital comparatively with thirty (30) apparently healthy persons as control subjects. Blood samples were collected from participants, they were analyzed for trace elements using atomic absorption spectrophotometer. The enzymes activities for serum glutamic oxaloacetic transamineses (SGOT), serum glutamic pyruvic transaminases (SGPT), and Alkaline phosphatase (ALP) were measured using the kinetic tests depending on the reaction with the substrates (2.4-dinitrophenylhydrazine and P. Nitro phenyl). The mean serum level of Magnesium, Zinc, in sickle cell disease patients were 13.1000 ± 1.89 mg/L, 0.40883 ± 0.095 mg/L respectively . Serum magnesium, zinc levels were significantly lower (p<0.05) in sickle cell disease patients when compared with the control. While liver enzymes were in a significant difference (P<0.01). SGOT reported low activity while ALP reported higher activity between the study groups. Serum trace elements levels was not age or sex dependent, as similar pattern of serum trace elements was observed in both male and female sickle cell disease patients. The age and sex distribution for SCD patients indicated tremendous increases in the number and percentage of the disease among males and females in the age between 2-9 years (66.7%, 57.5%) respectively. Seventy (70) patients with homozygous sickle cell (SS) disease, with both sexes. Their age ranged between 6 months to 17 years. Thirty persons (30) were used as control. Freshly obtained blood samples from patients and control were used to estimate biochemical parameters which include; liver function test (SGOT & SGPT and alkaline phosphatase), and trace element (Zinc, Magnesium). Different analytical (colorimetri, spectrophotomer) methods were used to determine biochemical parameters indicated above. Serum tracee