Dear all,
another elementary question:
Can it cause any problems when I prepare glycerol stocks from cultures
that were grown in double strength YPAD (YPD with additional adenin
added)? I use this 2xYPAD to grow pre-cultures for yeast transformation
and it would be very convenient to prepare glycerol stocks from the
pre-cultures immediately rather than setting up a separate culture in
YPD just for the preparation of glycerol stocks.
Any comments on that question are highly appreciated.
Anna
--
Dr. Anna Heidenblut (PhD)
Genome Integrity Group
Department of Molecular Oncology
Weatherall Institute of Molecular Medicine
Oxford University John Radcliffe Hospital,
Headley Way, Oxford, Oxfordshire OX3 9DS UK
Tel: 0044 / 1865 / 222419
anna.heidenblut At cancer.org.uk