How important is it to add exactly 1 volume?

I have been wondering how important is it to add exactly 1 volume of Phenol:Chloroform:Isoamyl alcohol when doing DNA extraction....for example, you have 800ul of your solution and you would add 1ml of Phenol:Chloroform (instead of 800ul), would it matter?

Edited by Wolverena, 20 February 2009 - 07:52 AM.

"You can give somebody a book on 'How to ride bike' and then test that person on that knowledge. Even if that person gets an "A", it doesn't mean that he or she can ride a bike."---this is what I am telling myself when I get a bad grade....as long as you don't loose your passion, you'll be fine.....V