Applications/Dilutions

This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).

Theoretical MW

19.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage

Store at -80C. Avoid freeze-thaw cycles.

Buffer

RIPA buffer

Lysate Details for Array

Type

Overexpression

Protein State

Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for POLR3H Overexpression Lysate (Native)

DNA-directed RNA polymerase III subunit 22.9 kDa polypeptide

DNA-directed RNA polymerase III subunit H

DNA-directed RNA polymerase III subunit RPC8

KIAA1665MGC29654

MGC111097

polymerase (RNA) III (DNA directed) polypeptide H (22.9kD)

RNA nucleotidyltransferase (DNA-directed)

RNA polymerase III subunit 22.9 kDa subunit

RNA polymerase III subunit C8

RNA polymerase III subunit RPC8

RPC8RPC22.9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Contact Information

Product PDFs

Calculators

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