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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is
committed to providing our customers around the world with the highest quality products.
In addition to direct selling in more than 20 countries in North America, Europe and
Asia/Pacific, Miltenyi Biotec also provides support for our customers through an
extensive distributor network covering dozens of additional countries.

As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is
committed to providing our customers around the world with the highest quality products.
In addition to direct selling in more than 20 countries in North America, Europe and
Asia/Pacific, Miltenyi Biotec also provides support for our customers through an
extensive distributor network covering dozens of additional countries.

REAlease® Fluorochrome Technology

Releasable antibodies for label-free cells after cell sorting

The next step in flexibility for cell sorting

Label-free cells for specific downstream applications

Recombinantly engineered for reproducible results

REAlease® Releasable Antibodies wins the technology prize!

We are proud to have been awarded the technology prize for our new REAlease Fluorochrome Technology at the 2018 Pharmaceutical Flow Cytometry & Imaging conference in Stevenage, UK. A big thank you to the 200 delegates who voted for us and the creative minds of our research and development team!

The basic principle

The benefits

• Epitope of a marker becomes available again for downstream applications that require free epitopes (e.g. cell culture experiments and cell analysis)• Fluorescence channel becomes available again for relabeling (e.g. microscopy applications)• Sequential flow sorting cycles for the isolation of specific cell subsets

A novel covalent conjugation approach allows for multimerization and fluorescent labeling of these antibody fragments. They form the so called REAlease Fluorochrome Complex which facilitates high-avidity cell binding that is comparable to conventional fluorochrome conjugates.

This novel and innovative conjugation chemistry allows for fast removal of the REAlease Fluorochrome Complex from the cell surface by adding the REAlease Release Reagent after cell sorting. This leads to disruption of the complex and thereby results in monomerization of the antibody fragments.

Due to their engineered low binding affinity, they spontaneously dissociate from the cell surface.

As a result, label-free cells can be obtained after cell sorting providing maximal flexibility for a wide range of applications.

Performance data of REAlease Releasable Antibodies

Staining and release performance of REAlease Antibodies. Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease Releasable Antibodies (A) or conventional antibodies (B) targeting CD4 and conjugated to different fluorochromes. All samples were also counter-stained with conventional CD3 antibodies. REAlease Antibodies were efficiently released using the REAlease Release Reagent (C) and successfully relabeled (D) using the same REAlease Releasable Antibodies targeting CD4. All samples were analyzed by flow cytometry on the MACSQuant® X.

Video

In this webinar learn about our brand-new REAlease Fluorochrome Technology. In just one step, it allows for removal of any labels from cells after cell sorting. Find out how this next step in flexibility could open up new possibilities for your downstream applications.