Although first-generation fuel ethanol is produced in Brazil from sugarcane-based raw materials with high efficiency, there is still little knowledge about the microbiology, the biochemistry and the molecular mechanisms prevalent in the non-aseptic fermentation environment. Learning-by-doing has hitherto been the strategy to improve the process so far, with further improvements requiring breakthrough technologies. Performing experiments at an industrial scale are often expensive, complicated to set up and difficult to reproduce. Thus, developing an appropriate scaled down system for this process has become a necessity. In this paper, we present the design and demonstration of a simple and effective laboratory-scale system mimicking the industrial process used for first generation (1G) fuel ethanol production in the Brazilian sugarcane mills. We benchmarked this system via the superior phenotype of the Saccharomyces cerevisiae PE-2 strain, compared to other strains from the same species: S288c, baker's yeast, and CEN.PK113-7D. We trust that such a system can be easily implemented in different laboratories worldwide, and will allow a better understanding of the S. cerevisiae strains that can persist and dominate in this industrial, non-aseptic and peculiar environment.

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HarvardRaghavendran, V., Basso, T., da Silva, J., Basso, L. och Gombert, A. (2017) A simple scaled down system to mimic the industrial production of first generation fuel ethanol in Brazil.

BibTeX @article{Raghavendran2017,author={Raghavendran, Vijayendran and Basso, T. P. and da Silva, J. B. and Basso, L. C. and Gombert, A. K.},title={A simple scaled down system to mimic the industrial production of first generation fuel ethanol in Brazil},journal={Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology},issn={0003-6072},volume={110},issue={7},pages={971-983},abstract={Although first-generation fuel ethanol is produced in Brazil from sugarcane-based raw materials with high efficiency, there is still little knowledge about the microbiology, the biochemistry and the molecular mechanisms prevalent in the non-aseptic fermentation environment. Learning-by-doing has hitherto been the strategy to improve the process so far, with further improvements requiring breakthrough technologies. Performing experiments at an industrial scale are often expensive, complicated to set up and difficult to reproduce. Thus, developing an appropriate scaled down system for this process has become a necessity. In this paper, we present the design and demonstration of a simple and effective laboratory-scale system mimicking the industrial process used for first generation (1G) fuel ethanol production in the Brazilian sugarcane mills. We benchmarked this system via the superior phenotype of the Saccharomyces cerevisiae PE-2 strain, compared to other strains from the same species: S288c, baker's yeast, and CEN.PK113-7D. We trust that such a system can be easily implemented in different laboratories worldwide, and will allow a better understanding of the S. cerevisiae strains that can persist and dominate in this industrial, non-aseptic and peculiar environment.},year={2017},keywords={Acid treatment, Cell recycling, Ethanol, Saccharomyces cerevisiae, Scale down, Viability, Yeast},}

RefWorks RT Journal ArticleSR ElectronicID 250790A1 Raghavendran, VijayendranA1 Basso, T. P.A1 da Silva, J. B.A1 Basso, L. C.A1 Gombert, A. K.T1 A simple scaled down system to mimic the industrial production of first generation fuel ethanol in BrazilYR 2017JF Antonie Van Leeuwenhoek International Journal of General and Molecular MicrobiologySN 0003-6072VO 110IS 7SP 971OP 983AB Although first-generation fuel ethanol is produced in Brazil from sugarcane-based raw materials with high efficiency, there is still little knowledge about the microbiology, the biochemistry and the molecular mechanisms prevalent in the non-aseptic fermentation environment. Learning-by-doing has hitherto been the strategy to improve the process so far, with further improvements requiring breakthrough technologies. Performing experiments at an industrial scale are often expensive, complicated to set up and difficult to reproduce. Thus, developing an appropriate scaled down system for this process has become a necessity. In this paper, we present the design and demonstration of a simple and effective laboratory-scale system mimicking the industrial process used for first generation (1G) fuel ethanol production in the Brazilian sugarcane mills. We benchmarked this system via the superior phenotype of the Saccharomyces cerevisiae PE-2 strain, compared to other strains from the same species: S288c, baker's yeast, and CEN.PK113-7D. We trust that such a system can be easily implemented in different laboratories worldwide, and will allow a better understanding of the S. cerevisiae strains that can persist and dominate in this industrial, non-aseptic and peculiar environment.LA engDO 10.1007/s10482-017-0868-9LK http://dx.doi.org/10.1007/s10482-017-0868-9OL 30