Subcellular localization of p29, a putative movement protein of pepper ringspot virus
Rodrigues, Kelly; Orílio, Anelise; Blawid, Rosana; Melo, Fernando; Nagata, Tatsuya
2015-01-01 00:00:00
Pepper ringspot virus (PepRSV) is a member of the genus Tobravirus . It possesses a bipartite single-strand RNA genome in a positive-sense polarity. The p29 protein is encoded by RNA 1 and is presumed to be the movement protein (MP) of this virus. In this study, the intracellular distribution of the p29 protein was analyzed by confocal microscopy. Transient expression of the PepRSV p29 protein fused to green fluorescent protein was observed as punctate spots localized next to the cell wall. This protein partially co-localized with the eCFP-tagged tobacco mosaic virus 30K MP, which is known to associate with plasmodesmata. This result suggests that the p29 protein is most probably the movement protein for PepRSV.
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Substitution of a single amino acid in the 2b protein of Pea early-browning virus affects nematode transmission

Vellios, E; Brown, DJF; MacFarlane, SA

Molecular biology of the tobraviruses

MacFarlane, SA

Transmission of Tobacco rattle virus isolate PpK20 by its nematode vector requires one of the two non-structural genes in the viral RNA 2

Hernández, C; Visser, PB; Brown, DJF; Bol, JF

Sequence comparison of RNA2 of nematode-transmissible and nematode-non-transmissible isolates of pea early-browning virus suggests that the gene encoding the 29 kDa protein may be involved in nematode transmission