[Histonet] re: Isopropyl vs Ethanol

From:

Maxim_71@mail.ru

Carl:
In mineral oil all tissues will "clear" as in old days,
when we used xylene. This is good test for adequate
dehydration and clearing I uses now.
Mineral oil very well mixes as with IPA at 50oC,
as with paraffin at 60oC or so.
Regards,
Maxim Peshkov
Russia,
Taganrog.
Carl Hobbs wrote:
> I never use ethanol for any histological-based
> system.
> I use IMS (Industrial methylated spirit/ Industrial
> Denatured Alcohol -IDA )
> Is IMS less/more expensive than IPA?
> I use IMS for pwax
> processing/rehydrating/dehydrating my pwax sections.
> ( us Brits will most likely use 74OP = ~99%: VWR
> list price is £135 for 25 L. However, every large
> Organisation buys it considerably cheaper.)
>
> Rene's suggestion to use IPA for pwax processing is
> a very good alternative: you do not need to use a
> "clearing " agent, like xylene.
> (Of course, your tissues will not "clear", lol: in
> the old days it was an indicator that the tissue was
> ready to move on to the next stage....these days we
> just rely on Protocols ;-)
>
> In my experience, IPA does not dissolve paraffin wax
> at RT BUT, at the 60C temp. of wax in a tissue
> processor/wax oven if processing by hand, IPA and wax
> are entirely miscible.
mailto:Maxim_71@mail.ru
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