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Microscopy represents one of the major breakthroughs of modern science. Since the early 17th century, scientists have made use of microscopes to make visible what was until then inaccessible to the human eye. The Ernst Ruska-Centre (ER-C) for Microscopy and Spectroscopy with Electrons specializes in the further development of these imaging techniques. Prof. Dr. Knut Urban, Prof. Dr. Joachim Mayer and Prof. Dr. Rafal E. Dunin-Borkowski will give an insight into this topic.