A Comparison of Two Extraction Methods for Food Oxalate Assessment

Ismail A. Al-Wahsh, Yan Wu, Michael Liebman

Abstract

Hyperoxaluria is a primary risk factor for the formation of calcium oxalate-containing kidney stones. Increased dietary oxalate intake and/or intestinal absorption may provide the critical quantity of additional oxalate that triggers the formation of kidney stones. The accurate determination of food oxalate is highly dependent on oxalate extraction, the first step in oxalate analysis. Potential problems include the possibility of elevated oxalate due to in vitro conversion from various oxalate precursors such as ascorbate and failure to dissolve all pre-existing calcium oxalate crystals. The primary objective was to compare the efficiency of the hot and cold extraction methods in extracting oxalate from 50 dry herb and 10 fresh fruit samples. Regardless of the method of extraction, leaves of Atriplex halimus and kiwifruit exhibited the highest concentrations of both total and soluble oxalate among the herbs and the fruits, respectively. The hot extraction method appeared to extract more total oxalate compared to the cold extraction method while there was no significant difference between the methods in efficiency of extracting soluble oxalate. The overall data suggested that the use of the hot acid method will yield a more accurate assessment of the total oxalate content of foods.

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