The aim of this study was to assess the effects of resistance training on ladders (RTL) on MMP-2 expression and blood lactate concentration [La-]. 30 male (3 months of age), albino rats were divided into 3 groups: sedentary control (SC, n=10), low resistance exercise training (Low-IntRT, n=10) and high-intensive exercise training (High-IntRT, n=10). Animals of High-IntRT were submitted to a progressively increasing overload in relation to body weight until exhaustion, while the Low-IntRT group performed the same exercise regimen with no external load. The program had a frequency of 3 times per week over 8 weeks. MMP-2 expression of tibialis anterior muscle and [La-] were measured. While there was a significant increase of MMP-2 (pro-form) in both groups, only High-IntRT significantly increased MMP-2 in active-form (p<0.05). Both trained groups exhibited an increase in [La-] when compared to controls, however, the increase in [La-] was significantly higher in the High-IntRT compared to Low-IntRT (p<0.05). Strong correlation was found between MMP-2 (active form) and [La-] in High-IntRT (r=0.91). RTL in using low and high-intensity exercise can serve as a model to demonstrate different responses of MMP-2 expression in an animal model. It appears active form expression of MMP-2 is modulated by exercise intensity.