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After fighting their way through many of Zoran Lazarević's soldiers in the previous chapter, Convoy, Nathan "Nate" Drake and his companion Elena Fisher find themselves in the monastery where Lazarević is holding Karl Schäfer hostage. Nate and Elena must fight their way through even more of Lazarević's soldiers in an effort to find Schäfer and catch up with Lazarević, who is now in control of the Phurba dagger, the apparent key to the lost city of Shambhala.

The Sanctuary, a map featured in the multiplayer component of Uncharted 2: Among Thieves, seems to be based on the monastery seen in this chapter and the subsequent chapter Reunion. Furthermore, The Sanctuary, an option in Uncharted 2: Among Thieves multiplayer Co-op Objective gameplay mode, also seems to be based on the events of the aforementioned two chapters.

Naughty Dog based the monastery that is seen in The Monastery and subsequent chapters on the Tiger's Nest monastery in Bhutan in the eastern Himalayas. The idea of including the Uncharted 2: Among Thieves take on the monastery came about during the game's pre-production, and was one of the earliest ideas developed for the game.[4]