Now available – sub-2μm Chiral UHPLC Columns from Daicel

Kinesis Australia is pleased to announce the availability of 2μm Chiral columns from our new supply partner, Daicel Corporation. These new columns are UHPLC versions of the renowned CHIRALPAK I-A/B/C columns and offer unrivalled speed and resolution.

Ultrafast Separations

As shown in Figure 1, the CHIRALPAK IA-U column effects the same separation as a CHIRALPAK IA-3 column, but in less than half the time (30 seconds versus 72 seconds). Ultrafast separations of chiral compounds are of utmost importance for rapid, high-throughput screening and method development phases, allowing a significant decrease in method development and analysis time, which leads to cost savings.

Higher Resolution Power

CHIRALPAK IA-U’s higher resolution power affords a much higher resolution than the standard 3-μm column, as shown in Figure 2. In this example, a 3.0 mm x 50 mmCHIRALPAK IA-U provides almost baseline separation of 2,2,2-trifluoro-1-(9-anthryl) ethanol (TFAE), while a CHIRALPAK IA-3 column of the same length is not adequate to show any separation.

Ease of Method Transfer

Figure 3 shows retention factor (k) data plotted for the separation of 10 compounds, using CHIRALPAK IC-3 and sub-2 μm IC-U columns. The excellent correlation coefficient (R) confirms that the (k) data for all 10 compounds are extremely well aligned. It follows that if a set of compounds has been separated on 3-μm columns, no new method development is required to switch to sub-2 μm columns.

Experimental and Discussion

CHIRALPAK IA and IC (3 μm) columns and CHIRALPAK IA-U and IC-U (< 2 μm) columns were used to obtain the data shown in Figures 1, 2 and 3.

About Daicel

Daicel Corporation is the global market leader in enantioselective chromatography, offering the most extensive portfolio of chiral stationary phases (CSPs) available on the market. Their renowned CHIRALPAK and CHIRALCEL columns have served scientists in the pharmaceutical, agricultural and food industries, universities and government agencies for many years. These columns offer excellent separations of chiral molecules into single enantiomers in research and development departments, QC laboratories and manufacturing. Daicel chiral columns offer excellent resolution of racemates, rapid and easy method development, plus durability and long service life. Their column range has recently been expanded to include UHPLC columns to offer faster, higher resolution separations.