Thermal Inactivation
(Fermentas)A simple, reversible way to a stop restriction reaction is by adding EDTA, which chelates Mg2+, thereby preventing catalysis. If further manipulations of the digested DNA are to be performed, the restriction endonuclease should be inactivated. Phenol/chloroform extraction and ethanol precipitation is an irreversible method for inactivation and removal of all restriction endonucleases...http://www.fermentas.com/techinfo/RE/restrthermina...Added: Tue May 14 2002, Hits: 158, Reviews: 0Write reviewCached