Lyophilized. Each vial contains 150 ng synthetic double-stranded DNA. Resuspend in 30ul autoclaved deionized water or TE buffer. To prevent contaminating samples and reagents, it is strongly recommended that the Positive Control is resuspended in a separate location from where PCR reactions are set up. Use a different pipette than one used for PCR set up. The Positive Control is not intended for quantitative purposes.

Alternate splice variants: Amplifies both variants to give the same size product

Suggested Reaction Conditions

For each 50ul Polymerase Chain Reaction mixture, 2ul of the primer pair stock solution should be used. Sufficient reagent is supplied for 25 separate amplification reactions of the gene fragment.

Thermocycling Conditions

A typical 50ul PCR would involve a 4 minute denaturation step at 94ºC, followed by 30-35 cycles of 45 seconds at 94ºC, 45 seconds at 55ºC and 45 seconds at 72ºC, with a final 10 minute extension step at 72ºC.

Storage and Stability

The Primer Pair resuspended in water or TE buffer is stable for up to one year at -20°C in a non-frost free freezer. Aliquot in single use portions. Do not use past the expiration date above. Avoid repeated freeze-thaw cycles.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.