Figure 2: Chromatin Comet Assay (ChCA) performance and the logic behind its results. (a) A scheme of the method of (ChCA) is presented with subsequent crucial steps marked with numbers. The method is a modification of the conventional Comet Assay technique and allows easy and fast visualization of higher-order chromatin structures at a single cell level. (b) Representative images of wild type and hho1delta mutant comets. The white bar stands for 20 μm.