Leptin detection

Leptin is an adipose-derived hormone that acts through a signaling pathway to regulate energy (food) intake and expenditure, maintaining steady levels of adipose tissue mass. Leptin was discovered in studies of obesity in mice. In humans, leptin is a 16-kDa protein, encoded by the LEP gene, and is expressed mostly in white adipose tissue. Leptin acts through the leptin receptor, which exists as multiple isoforms. In addition to its role in adipose tissue, leptin regulates immune and inflammatory responses, hematopoiesis, angiogenesis, and wound healing. Mutations in the LEP gene cause severe to morbid obesity and hypogonadism and are associated with the development of type 2 diabetes. Leptin is also known as obesity protein (OB), obesity factor, OBS, and LEPD.

Leptin is an adipose-derived hormone that acts through a signaling pathway to regulate energy (food) intake and expenditure, maintaining steady levels of adipose tissue mass. Leptin was discovered in studies of obesity in mice. In humans, leptin is a 16-kDa protein, encoded by the LEP gene, and is expressed mostly in white adipose tissue. Leptin acts through the leptin receptor, which exists as multiple isoforms. In addition to its role in adipose tissue, leptin regulates immune and inflammatory responses, hematopoiesis, angiogenesis, and wound healing. Mutations in the LEP gene cause severe to morbid obesity and hypogonadism and are associated with the development of type 2 diabetes. Leptin is also known as obesity protein (OB), obesity factor, OBS, and LEPD.

ELISAs for leptin detection

These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to leptin: one is precoated on the ELISA plate, and the other is HRP-conjugated. These assay kits can be used to measure soluble human, mouse, or rat leptin protein in serum, EDTA plasma, or cell culture supernatants.

Antibodies for leptin detection

These products are affinity-purified IgG antibodies that recognize human, mouse, or rat leptin protein. The antibodies were raised in rabbit using synthetic peptides, and can be used for Western blot (WB) detection of leptin protein.

The recessive obesity mutation, ob, identified in 1950, results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. Freidman's group at Rockefeller University originally identified the obesity gene that encodes for Leptin, a 16 kDa secreted protein produced by the ob gene, in 1994. Adipose tissue produces Leptin and releases it into the bloodstream. As fat deposits grow, blood Leptin levels tend to increase. It has been suggested that Leptin acts as a lipostat, increasing as fat gets deposited into adipocytes. It is has also been reported that Leptin acts as a hormone instructing the brain to stop food consumption and to increase activity. The protein has also been shown to signal and probably control the onset of puberty. Furthermore, a recent study shows that Leptin acts as a potent inhibitor of bone formation through the central nervous system.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

The recessive obesity mutation, ob, identified in 1950, results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. Freidman's group at Rockefeller University originally identified the obesity gene that encodes for Leptin, a 16 kDa secreted protein produced by the ob gene, in 1994. Adipose tissue produces Leptin and releases it into the bloodstream. As fat deposits grow, blood Leptin levels tend to increase. It has been suggested that Leptin acts as a lipostat, increasing as fat gets deposited into adipocytes. It is has also been reported that Leptin acts as a hormone instructing the brain to stop food consumption and to increase activity. The protein has also been shown to signal and probably control the onset of puberty. Furthermore, a recent study shows that Leptin acts as a potent inhibitor of bone formation through the central nervous system.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

The recessive obesity mutation, ob, identified in 1950, results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. Freidman's group at Rockefeller University originally identified the obesity gene that encodes for Leptin, a 16 kDa secreted protein produced by the ob gene, in 1994. Adipose tissue produces Leptin and releases it into the bloodstream. As fat deposits grow, blood Leptin levels tend to increase. It has been suggested that Leptin acts as a lipostat, increasing as fat gets deposited into adipocytes. It is has also been reported that Leptin acts as a hormone instructing the brain to stop food consumption and to increase activity. The protein has also been shown to signal and probably control the onset of puberty. Furthermore, a recent study shows that Leptin acts as a potent inhibitor of bone formation through the central nervous system.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

The recessive obesity mutation, ob, identified in 1950, results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. Freidman's group at Rockefeller University originally identified the obesity gene that encodes for Leptin, a 16 kDa secreted protein produced by the ob gene, in 1994. Adipose tissue produces Leptin and releases it into the bloodstream. As fat deposits grow, blood Leptin levels tend to increase. It has been suggested that Leptin acts as a lipostat, increasing as fat gets deposited into adipocytes. It is has also been reported that Leptin acts as a hormone instructing the brain to stop food consumption and to increase activity. The protein has also been shown to signal and probably control the onset of puberty. Furthermore, a recent study shows that Leptin acts as a potent inhibitor of bone formation through the central nervous system.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

A typical standard curve obtained using the Human Leptin ELISA Kit

A typical standard curve obtained using the Human Leptin ELISA Kit.

27160A

Mouse Leptin Assay Kit - IBL

96-Well

$664.00

The recessive obesity mutation, ob, identified in 1950, results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. Freidman's group at Rockefeller University originally identified the obesity gene that encodes for Leptin, a 16 kDa secreted protein produced by the ob gene, in 1994. Adipose tissue produces Leptin and releases it into the bloodstream. As fat deposits grow, blood Leptin levels tend to increase. It has been suggested that Leptin acts as a lipostat, increasing as fat gets deposited into adipocytes. It is has also been reported that Leptin acts as a hormone instructing the brain to stop food consumption and to increase activity. The protein has also been shown to signal and probably control the onset of puberty. Furthermore, a recent study shows that Leptin acts as a potent inhibitor of bone formation through the central nervous system.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

A typical standard curve obtained using the Mouse Leptin ELISA Kit

A typical standard curve obtained using the Mouse Leptin ELISA Kit.

27295A

Rat Leptin Assay Kit - IBL

96-Well

$664.00

The recessive obesity mutation, ob, identified in 1950, results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. Freidman's group at Rockefeller University originally identified the obesity gene that encodes for Leptin, a 16 kDa secreted protein produced by the ob gene, in 1994. Adipose tissue produces Leptin and releases it into the bloodstream. As fat deposits grow, blood Leptin levels tend to increase. It has been suggested that Leptin acts as a lipostat, increasing as fat gets deposited into adipocytes. It is has also been reported that Leptin acts as a hormone instructing the brain to stop food consumption and to increase activity. The protein has also been shown to signal and probably control the onset of puberty. Furthermore, a recent study shows that Leptin acts as a potent inhibitor of bone formation through the central nervous system.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

More Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion.

Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).