AlphaScreen SureFire GAPDH Assay Kit - 10,000 Points

The AlphaScreen®SureFire® total GAPDH assay is a sandwich immunoassay for quantitative detection of GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a cellular normalization assay. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

The HV 96-well kits contain enough reagents to run 100 wells in 96-well format, using a 50 µL reaction volume.

The 500 point kit contains enough reagents to run 500 wells in 384-well format, using an 11 µL reaction volume.

The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using an 11 µL reaction volume.

The 50,000 point kit contains enough reagents to run 50,000 wells in 384-well format, using an 11 µL reaction volume.

In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.