Description

Containing 57 thoroughly class-tested exercises, Laboratory Experiments in Microbiology, Tenth Edition, provides engaging labs with instruction on performing basic microbiology techniques and applications for undergraduate students in diverse areas, including the biological sciences, allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various pre-professional programs. The perfect companion to Tortora/Funke/Case’s Microbiology: An Introduction or any introductory microbiology text, the Tenth Edition features an updated art program and a full-color design, integrating valuable micrographs throughout each exercise. Additionally, many of the illustrations have been re-rendered in a modern, realistic, three-dimensional style to better visually engage students. Laboratory Reports for each exercise have been enhanced with new Clinical Applications questions, as well as questions relating to Hypotheses or Expected Results. Experiments have been refined throughout the manual and the Tenth Edition includes an extensively revised exercise on transformation in bacteria using pGLO to introduce students to this important technique.

Table of Contents

Introduction

I. MICROSCOPY

1. Use and Care of the Microscope

2. Examination of Living Microorganisms

II. Handling Bacteria

3. Microbes in the Environment

4. Transfer of Bacteria: Aseptic Techniques

III. STAINING METHODS

5. Preparation of Smears and Simple Staining

6. Negative Staining

7. Gram Staining

8. Acid-Fast Staining

9. Structural Stains (Endospore, Capsule, Flagella)

10. Morphologic Unknown

IV. CULTIVATION OF BACTERIA

11. Isolation of Bacteria by Dilution Techniques

12. Special Media for Isolating Bacteria

V. MICROBIAL METABOLISM

13. Carbohydrate Catabolism

14. Fermentation

15. Protein Catabolism, Part 1

16. Protein Catabolism, Part 2

17. Respiration

18. Unknown Identification and Bergey’s Manual

VI. MICROBIAL GROWTH

19. Oxygen and the Growth of Bacteria

20. Determination of a Bacterial Growth Curve: The Role of Temperature