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Microbiology Dilution Series Demonstration

Here's a nice demonstration video by Dr. Conor O'Bryne from the Dept. of Microbiology at National University of Ireland, Galway, showing how to perform a serial dilution on a liquid food sample (in this case raw unpasteurised milk). The raw milk sample is diluted down to 1/1000 in a sterile diluent (Ringers solution). 1ml samples of each dilution are then used to prepare 2 sets of pour plates. A 0.1ml sample from the 1/10 and the 1/100 dilutions is used to generate spread plates.