Figure 1: Viability of macrophages, under either 10 or 30 mM glucose, by MTT assay. RAW 264.7 cells were plated at 8 × 104/well and were treated with 10, 50 or, 100 nM of NT (a) or in combination with 1 μg/mL of LPS (b) for 7days. After, 1, 3, 5, or 7 days of incubation, MTT assay was performed as described in “Materials and Methods.” Absorbance quantification was performed using a microplate reader at 570 nm, with a reference wavelength of 620 nm. Results are presented as mean ± SEM of three independent experiments.