Characterization of Poly(3,4-ethylenedioxythiophene) as a Novel Optically-Transparent Electrode Material for Sensitive Detection of Neurotransmitters

Presider(s):

Autry, Lara P

Start Time:

03:35 PM ( Slot # 6 )

Date:

Tuesday, March 13th, 2012

Location:

311B

Keywords:

Biosensors, Electrode Surfaces, Microelectrode, Neurochemistry

Co-Authors

Name

Affiliation

Heien, Michael L

University of Arizona

Larsen, Simon T

Technical University of Denmark

Taboryski, Rafael J

Technical University of Denmark

Abstract Content

Here, we present a novel use, and demonstrate the efficacy of an electrode material with potential for greater biocompatibility, transparency, and durability than other commonly used electrode materials in neurochemistry. Poly-3,4-ethylenedioxythiophene (PEDOT), an optically transparent intrinsically conductive polymer, has been investigated as a novel electrode material for the detection of dopamine and other monoamine neurotransmitters by background-subtracted fast-scan cyclic voltammetry and amperometry. The counter ion used in conjunction with the PEDOT material is iron(III) p-toluene sulfonate. PEDOT was spin-coated and patterned on TOPAS substrates, and conductivity and sheet resistance were measured. To determine that the fabricated sensor is functional, electron transfer kinetics were determined for ferrocene carboxylic acid, dopamine, 3,4-dihydroxyphenylacetic acid, homovanillic acid, norepinephrine, epinephrine, L-3,4-dihydroxyphenylalanine, 5-hydroxyindoleacetic acid, and serotonin. In addition, the stability and selectivity of the electrode material was characterized in simulated neuronal environments. Absorption of the material in the visible light range was measured. A model of the electrode-solution interface was constructed using data from electrochemical impedance spectroscopy. To test the biocompatibility of the sensor, mast cells from mice were cultured on PEDOT and exocytosis was measured.