Cornmeal, Sucrose and Yeast Medium

This recipe came from Harvard Biolabs, although they now use a dextrose medium. It has not been used at Bloomington. The cooking instructions assume that your are cooking in a steam-jacketed kettle with an automatic mixer.

Ingredients

Water

8.5

liters

Agar

79

grams

Torumel yeast

275

grams

Cornmeal

520

grams

Granulated sugar

1,100

grams

p-Hydroxy-benzoic acid methyl ester

23.8

grams

95% ethanol

91.8

milliliters

*adjust agar concentration according to the gel strength of your agar

Instructions

Boil water.

Add agar, stirring constantly.

After agar has dissolved, add yeast, cornmeal and sugar and continue stirring until mix boils again.