Interacting insulators from the Drosophila melanogaster bithorax complex can form independent expression domains

Interacting insulators from the Drosophila melanogaster bithorax complex can form independent...
Kyrchanova, O.; Ivlieva, T.; Georgiev, P.
2011-12-08 00:00:00
Regulatory region of three bithorax complex genes, Ultrabithorax (Ubx), abdominal-A (abd-A), and Abdominal-B (Abd-B) can be divided into nine iab domains, capable of directing expression of one of the genes in certain abdominal parasegment of Drosophila. In the Abd-B regulatory region, three insulators were identified, including Fab-7 and Fab-8, which flanked the iab-7 domain, and Mcp, which separated the Abd-B and abd-A regulatory regions. It was suggested that boundary insulators formed a barrier between active and repressed chromatin. In the present study, using the yellow and white reporter genes and different combinations of known insulators, Mcp, Fab-7, and Fab-8, it was demonstrated that only specific interaction of two insulators was capable of isolation of active and repressed chromatin, i.e., the formation of independent expression domains.
http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.pngRussian Journal of GeneticsSpringer Journalshttp://www.deepdyve.com/lp/springer-journals/interacting-insulators-from-the-drosophila-melanogaster-bithorax-CMR07jyLkd

Interacting insulators from the Drosophila melanogaster bithorax complex can form independent expression domains