Brazil is one of the main centers of genetic variability dispersion of the Passiflora genera. Its self
incompatibility as well as disease incidence in its leaves and root system and, deforestation and
monocultivation, promote loss of genetic material. Considering the risk of genetic erosion, the
conservation of the variability in germplasm banks, which is of great interest in plant breeding, is
necessary. Studies regarding the type of explant and concentration of the culture media are necessary in
order to determine protocols of establishment and in vitro conservation of passion-fruit germplasm.
The objective of the present work was to evaluate the influence of the salt and nutrient concentration in
the MS culture medium and types of explants in the establishment and growth of the Passion fruit species:
Passiflora giberti N. E.Brown, P. edulis Sims and P. laurifolia L. Each Passiflora species presented its own
characteristics regarding in vitro development. The complete MS medium and nodal segments the second
axilliary bud promoted better development of the genotypes studied.

Brazil is one of the main centers of genetic variability dispersion of the Passiflora genera. Its self
incompatibility as well as disease incidence in its leaves and root system and, deforestation and
monocultivation, promote loss of genetic material. Considering the risk of genetic erosion, the
conservation of the variability in germplasm banks, which is of great interest in plant breeding, is
necessary. Studies regarding the type of explant and concentration of the culture media are necessary in
order to determine protocols of establishment and in vitro conservation of passion-fruit germplasm.
The objective of the present work was to evaluate the influence of the salt and nutrient concentration in
the MS culture medium and types of explants in the establishment and growth of the Passion fruit species:
Passiflora giberti N. E.Brown, P. edulis Sims and P. laurifolia L. Each Passiflora species presented its own
characteristics regarding in vitro development. The complete MS medium and nodal segments the second
axilliary bud promoted better development of the genotypes studied.