About this book

Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers many necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (the isolation and fractionation chapters), to characterization of the separated organelles.

Contents

1. Homogenization of tissues and cells; 2. Isolation of subcellular fragments; 3. Isolation and characterization of nuclei and nuclear subfractions; 4. Subcellular fractionation of mitochondria; 5. Isolation and characterization of peroxisomes; 6. Lysosomes and endocytosis; 7. The membranes of the secretory and exocytic pathways; 8. Isolation and purification of functionally intact mitochondria and chloroplasts from plant cells; 9. Ribosomes and polysomes; 10. Electron microscopy of organelles

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