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WB: Colorimetric - use at a dlution of 1/1,000 - 1/10,000. Chemiluminescent - use at a dilution of 1/1,000 - 1/30,000. IHC: use at a dilution of 1/200 - 1/2,000. ICC: use at a dilution of 1/200 - 1/2,000. ELISA: Coating - use at a dilution of 1/100 - 1/500. Primary - use at a dilution of 1/1,000 - 1/30,000. Optimail dilutions should be determined by the end user.