FEATURED NEW PRODUCTS

what they are Human Corneal Epithelial Cells (HCECs) from Gibco® are normal human cells isolated from the progenitor-rich limbal region of the eye, and are cryopreserved at the end of the secondary culture. HCECs are ideal for research on corneal biology, including inflammation and wound healing, investigating the effects of chemicals and components in consumer products, and other studies of ocular function.

what they offer

Guaranteed to be ≥70% viable upon thawing and to contain ≥500,000 viable cells/vial

Guaranteed to reach at least 12 population doublings after thawing when using Keratinoctye-SFM

how they work Cryopreserved HCECs are optimal for investigating the cellular, molecular, and biochemical processes that occur in the cornea and have been optimized for use with Gibco® Keratinocyte Serum-Free Medium. HCECs closely mimic the in vivo state and enable generation of physiologically relevant data. Limbal tissue is known to be enriched for corneal epithelial progenitor cells. Our robust isolation and expansion process yields large single-donor lots, to help ensure availability of consistent product.

what it is The c-Met receptor is a potential therapeutic target in some cancer models. c-Met is activated by phosphorylation at tyrosine residues, and can be activated by epidermal growth factor receptor (EGFR) without production of hepatocyte growth factor (HGF), inducing signaling cascades through a ligand-independent event.

what it offers

ABfinity™ antibodies—recombinant antibodies enable consistent results, time after time

New antibodies released every month

how it works ABfinity™ recombinant rabbit monoclonal antibodies help ensure consistent antibody performance lot after lot, so you don’t have to revalidate dilutions for your experiments when you order more. c-Met ABfinity™ Recombinant Rabbit Monoclonal Antibodies are available for total c-Met and c-Met phosphorylated at tyrosine 1230, and are validated in applications ranging from western blotting to immunofluorescence. ABfinity™ Recombinant Rabbit Monoclonal Antibodies are also available for EGFR.

Measure Phosphorylation of Tau Proteins — Tau [pT231] Human ELISA Kit

what it is The Invitrogen™ Tau [pT231] Human ELISA Kit is designed to detect and quantify the level of threonine-231 phosphorylation in tau proteins from human cell lysates and cerebrospinal fluid (CSF). This validated and complete phosphoELISA™ kit contains all the buffers and reagents you need to quickly and easily perform the assay and obtain reliable results.

what it offers

Validated on SH-SY5Y cell lysates and CSF

Rapid 4-hour protocol

Precoated removable 8-well strips

how it works The Invitrogen™ Tau [pT231] Human ELISA Kit is a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA).

A monoclonal antibody specific for tau (regardless of phosphorylation state) has been coated onto the wells of the microtiter strips provided. Samples are pipetted into these wells. During the first incubation, the tau antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for tau [pT231] phosphorylation is added to the wells. During the second incubation, this antibody serves as a detection antibody by binding to the immobilized tau protein captured during the first incubation. After removal of excess detection antibody, a horseradish peroxidase–labeled anti-rabbit IgG (anti-rabbit IgG HRP) is added. This binds to the detection antibody to complete the four-member sandwich. After washing, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of tau [pT231] present.

NEW APPLICATIONS

Many proteins can be regulated by the attachment of
O-linked β-N-acetylglucosamine residues (
O-GlcNAc) to serine and threonine amino acids, altering their functionality in a manner similar to phosphorylation. In a recent publication, Sakabe et al. [1] utilized a combination of click chemistry and mass spectroscopy to determine the
O-GlcNAc modification sites on histones.

In this study, the researchers isolated and digested the histones, yielding peptides. These peptides were then treated with phosphatase, leaving O-GlcNAc modifications intact. Then they used a product similar to the Click-iT® O-GlcNAc Enzymatic Labeling System, wherein β-1,4-galactosyltransferase (Gal-T1) transfers azido-modified galactose (GalNAz) from UDP-GalNAz to O-GlcNAc residues on target proteins in a highly specific reaction. For detection, the researchers used a product similar to the Click-iT® Biotin Protein Analysis Detection Kit followed by mass spectrometry to detect the peptides and thus the exact O-GlcNAc modification sites.

This study illustrates the specificity and precision of click chemistry used to study the elusive sites of O-GlcNAc modifications. The click reaction is highly specific and the reaction product contains an irreversible covalent bond, making the technique ideal for accurate downstream identification with mass spectrometry.

All six of our anti-GFP antibodies are suited for detection of native GFP, GFP variants, and most GFP fusion proteins. The affinity-purified anti-RFP antibody is used to detect native TagRFP and most fusion proteins derived from Entacmaea quadricolor. The RFP proteins derived from E. quadricolor have 2.8 times the quantum yield of mCherry, and more importantly, they remain as monomers, making it more likely that the fusion protein will function properly in the cell [1].

Buzzworthy

Recent studies have revealed that embryonic stem cell (ESC) proliferation and differentiation can be influenced by exogenous mechanical forces. To further investigate the effect of these forces on mouse ESCs (mESCs), Hemsley et al. combined atomic force microscopy with laser scanning confocal microscopy to characterize morphological and biochemical responses of mESCs to precisely delivered nanomechanical forces. The researchers identified two morphologically distinct subpopulations of mESCs: round and flattened. Using phalloidin conjugated to Alexa Fluor® 546 or Alexa Fluor® 488, immunofluorescence imaging of the cytoskeleton revealed that round cells, but not flattened cells, exhibited blebbing in response to mechanically induced forces. Flattened cells were characterized by a more highly developed cytoskeleton and a stronger mechanical link between the plasma membrane and cytoskeleton. These results suggest that mechanosensitivity of ESCs at the earliest stages of differentiation may play an important role in ESC biology.