The new species, Daedalea ryvardenica, presents irregular hymenophore, generative hyphae with irregular thickened walls, skeletal hyphal endings forming an irregular palisade within the hymenium, and presents the largest basidiospores in the genus, with a ventral concavity next to the apiculous and tapering apex. A taxonomic discussion of the morphological characters of Daedalea, the hyphal system and the shape of the basidiospores, and a key of Neotropical Daedalea species are presented.