Target details

Target details

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Background

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.Synonyms: MAP1LC3A, microtubule-associated protein 1 light chain 3 alpha, RP11-346K17.1, LC3, LC3A, MAP1ALC3, MAP1BLC3, MAP1 light chain 3-like protein 1, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, OTTHUMP00000030698, autophagy-relate

LC3 and the CLN3-encoded protein, battenin, co-immunostaining. Wild-type and mutant cerebellar cells were costained with ABIN388464 anti-LC3 (APG8a) Abgent antibody (Red) and a monoclonal anti-battenin antibody(Green) and images were captured by confocal microscopy. Note the numerous larger vesicular structures typical of mature Avs stained with both the LC3 and battenin antibodies in wild-type cells (arrows) which are rare in mutant cells. Blue=DAPI.

LC3 and the CLN3-encoded protein, battenin, co-immunostaining. Wild-type and mutant cerebellar cells were costained with ABIN388464 anti-LC3 (APG8a) Abgent antibody (Red) and a monoclonal anti-battenin antibody(Green) and images were captured by confocal microscopy. Note the numerous larger vesicular structures typical of mature Avs stained with both the LC3 and battenin antibodies in wild-type cells (arrows) which are rare in mutant cells. Blue=DAPI.

LC3 and the CLN3-encoded protein, battenin, co-immunostaining. Wild-type and mutant cerebellar cells were costained with ABIN388464 anti-LC3 (APG8a) Abgent antibody (Red) and a monoclonal anti-battenin antibody(Green) and images were captured by confocal microscopy. Note the numerous larger vesicular structures typical of mature Avs stained with both the LC3 and battenin antibodies in wild-type cells (arrows) which are rare in mutant cells. Blue=DAPI.

He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).