This antibody gave a positive signal in the following whole cell lysates: Jurkat; K562; MCF7; HepG2.
This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.

製品の特性

製品の状態Liquid

保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

バッファーpH: 7.40Preservative: 0.02% Sodium azideConstituent: PBSNote: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.

関連製品

アプリケーション

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション

Abreviews

特記事項

WB

Use a concentration of 1 µg/ml. Detects a band of approximately 183 kDa (predicted molecular weight: 183 kDa). Abcam recommends using milk as the blocking agent - 5%

ターゲット情報

機能Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Predicted band size : 183 kDaObserved band size : 183 kDaAdditional bands at : 120 kDa,150 kDa,42 kDa,55 kDa,66 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 8 minutesAbcam recommends using milk as the blocking agent - 5%.
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab125297 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-Topoisomerase II beta antibody (ab125297) 使用論文

ab125297
has not yet been referenced specifically in any publications.