Just add 3 drops of milk / blood serum in the casette and get the result in 5 minutes

The kit can be stored in room temperature and is easy to transport

Expiration of the kit is two years from the date of manufacture.

BOVIPREG - One Step Pregnancy Test Cassette

This test evaluates early pregnancy and suspected pregnancy.

This Kit detects pregnancy for both beef and dairy cows. This kit also evaluates early pregnancy and suspected pregnancy in sheep and goat.

To detect early pregnancy following procedure should be followed:

Perform one test between 18th AND 22nd day post insemination to detect whether the animal is open or is in luteal phase. If found positive then perform one more test on either 23rd OR 24th day post insemination. If this 2nd test also shows positive then the animal’s pregnancy is confirmed. If the 2nd test shows negative then the animal is considered NOT pregnant, even though the test performed between 18th AND 22nd day post insemination is positive. If the first test performed between 18th AND 22nd day shows negative then there is no need to perform this test on either 23rd OR 24th day as the animal is NOT pregnant.

If the testing between 18th AND 22nd day post insemination cannot be performed or missed somehow then the test should be performed on BOTH 23rd AND 24th day. If the test performed on 23rd day shows positive but shows negative on 24th day then the animal is NOT pregnant. If the test on 23rd day shows negative then the animal is NOT pregnant and there is no need to do that test on the 24th day. If the test shows positive on both 23rd AND 24th day then the animal’s pregnancy is confirmed.

Beyond 24th day and upto 150th day we recommend 5 tests in 5 consecutive days to detect pregnancy for the animal whose insemination date is not known or if testing could not be performed between 18th and 22nd OR 23rd and 24th day post insemination. If any one of the 5 tests shows negative then the animal is NOT pregnant and there is no need to perform further tests. But if all tests performed in 5 consecutive days show positive then the pregnancy is established.

The Kit does not require refrigeration for storage. It is easy to transport and can be stored in room temperature.

Reproductive physiology of Cow, sheep and goat are almost same except their estrus and gestation periods. In ewe(sheep) and doe(goat) 98% accuracy for detecting non-pregnancy is achieved by using this test at day 18-21 days after mating and gives 97% accuracy of pregnancy, however embryonic death and other pathological condition may give some false positive cases.

INTENDED USE

One of the aims of beef/diary cow feeding management is to improve milk output and shortening the period of non-pregnancy.

PRINCIPLE

Detection of pregnancy in beef/dairy cow, goat and sheep offer valuable information to producers. For a pretty long time, dairy/beef farmers and veterinarians have been looking for a simple, rapid and effective early pregnancy detection method, which is important to improve better beef/dairy management and profitability. If the pregnancy is not promptly detected after insemination, this will delay the inter-calving period and decrease the propagation rate and milk output. Thus the cost of feeding management will be controlled for those non-pregnant cows. Also, the non-pregnant cows can be re-inseminated immediately so that the farmer does not loose at least one more opportunity to re-breed their animal. Early pregnancy detection shortens the period of non-pregnancy by way of effective treatment or culling and thus increases the profitability of maintaining a herd.

In case of positive pregnancy the beef/dairy cows will get better care during gestation period. Efficient and smart pregnancy detection increases the success rate of pregnancy in beef/dairy herd. So a study on the diagnostic skill for early pregnancy of beef/diary cow is very important for the development of this industry.

MODE OF ACTION

Progesterone is a steroid hormone. The concentration of progesterone indicates the activities of ovarian function of a cow. The estrous (HEAT) cycle of cow is 21 days on an average. Progesterone is in a low-level at the beginning of the estrous cycle, thereafter it rises gradually, reaching a more high-level in the mid period of estrous cycle. Progesterone level in non-pregnant cow begins to drop at the lowest level on the 17th day after insemination, while it is at a high level in a pregnant dairy cow’s blood and milk. This product is designed and developed on the basis of Progesterone hormonal levels in the milk.

If only one red line appears, it indicates that the cow is not pregnant, progesterone is in the lowest level at this moment. It is recommended to make the best use of this time to have the diary cow mate.

If two red lines appear it indicates the cow is pregnant. It should get careful nursing and gestation management. The test theory is based on variation of Progesterone hormone in the estrous cycle, and it determine the early pregnancy of dairy/beef cow by detecting Progesterone in the end of estrous cycle. The test strip employs colloidal gold an Immuno-chromatographic technology for early pregnancy diagnosis of diary/beef cow, with characteristics of high accuracy, easy to operate, fast result, room temperature storage, easy to carry, safe to mother and baby cow.

SCOPE OF USE

The test can be used to determine the early pregnancy of beef/diary cow, re-examine and guide the artificial insemination at the right time.

SPECIFICITY

Specificity of this test was determined from cross reaction studies with FSH (100µg/mL), E2(10µg/mL), PRL(100µg/mL), LH(25mIU/ml), DHT(10µg/mL), tetraborane(10µg/mL) and H2O. No cross reactivity was observed.

HOW TO USE

Because the beef/diary cow’s estrous cycle is 21 days, it is suggested that one test be performed between 18-22 days AND one test on either 23rd OR 24th day post insemination. So, perform two tests to know the pregnancy status. In suspected case, like where insemination time is unknown, perform the test every day for 5 continuous days. One negative result in any of these 5 tests indicates that the animal is not pregnant and progesterone is at the lowest level at this moment. It is recommended to make use of the time to have the diary animal mate. It can improve conception rate. If positive results are obtained on all 5 tests, performed in 5 consecutive days then the animal is pregnant. It should then get careful nursing and gestation management. Pregnant beef/dairy cow can be reexamined at any time.

SPECIMEN COLLECTION AND PREPARATION

MILK : The milk should be fresh, whole milk. It’s best if you discard the first couple of squirts and then collect a small amount of milk—about 5 to 10 ml in a cup. The sample should be well mixed before you use it for the test. Sometimes milk with high viscosity prevents its spreading to all areas of the kit. So, the specimen could not go up to the whole membrane and apparently stopped in the middle of the test . To solve this problem, you will need to keep the milk still (at rest) for half an hour after collection, and then draw the milk from the bottom layer using the dropper and use that milk on the test kit. Please note that the milk cream usually floats on the surface.

BLOOD SERUM : Blood withdrawn and collect it in a collection tube, left it in horizontal position for 30 minutes, then left it vertical for another 30 minutes, till blood clot. A clear liquid appear over the clot. Collect the serum in the dropper and pour 3-4 drops on the circular well.

ASSAY PROCEDURE

(1)Remove the test device and dropper from its foil wrapper by tearing along the slice.

(2)Using the dropper, withdraw the milk/blood serum sample collected in a cup and slowly dispense 3~5 drops into the circular sample well. The sample liquid moves slowly to the other end due to the capillarity. You will see one or two red lines in the result window

(3) Read results at 5 minutes..

INTERPRETATION OF RESULTS

Positive: If both T band and C band are visible, the test result is Positive, which means the cow is pregnant or in middle period of estrous cycle.

Negative: If only C line is visible, the test result is negative, which indicates the cow is not pregnant.

Invalid: When control line does not appear on the membrane, the test is invalid due to improper test procedure or deterioration of reagents. It is recommended that the test be repeated.

PRECAUTION

(1) Test according to the law of the estrous cycle strictly.

(2) For single use only, do not reuse the test devices.

(3) Do not keep the strip or cassette in the air for a long time after unpacking.

(4) While performing the test, make sure that the surface of sample should not exceed the “MAX” line of strip.

(5) Do not use test kit after expiry date.

VALIDITY AND STORAGE

The period of the validity is 24 months. Store as packaged in the sealed pouch under the room temperature.

FAQ :

1. Why BOVIPREG considered a one- step pregnancy test kit ?

The kit requires only one step, and that is to add samples, like milk or blood serum in the circular well on the cassette, and see the result in the window site of the cassette in a short while.

2. How much time will it take to get the result ?

It takes only 5 minutes to get the result. However if the sample used is milk and if its viscosity is high, then the result may become visible after 10 minutes.

3. How to detect early pregnancy ?

One test needs to be performed between 18th and 22nd day after insemination. If found positive then the test is repeated on either 23rd or 24th day and if positive then pregnancy is confirmed. One negative result confirms animal is NOT pregnant.

4. How do you test pregnancy after 24th day post insemination ?

After 24th day of insemination we recommend that you perform 5 consecutive tests on 5 days. If the test is performed for 5 consecutive days then one negative result during this period will prove that the cow is not pregnant. If all 5 days result show positive then pregnancy is established

5. Can the test be effective in any stage of gestation period ?

The test is optimized to detect pregnancy status up to 150 days of gestation period

The milk should be fresh and whole, for early pregnancy detection. Morning milk is ideally suited for the test because the hormonal level is higher in the morning. It’s best if you discard the first couple of squirts and then collect a small amount of milk—about 5 to 10 ml in a cup. The sample should be well mixed before you use it for the test. Sometimes milk with high viscosity may not be able to spread to all areas of the kit to cover the whole membrane and stop in the middle of the test. To avoid this problem, you will need to keep the milk still (at rest) for half an hour after collection, and then draw the milk from the bottom layer using the dropper and use that milk on the test kit. Please note that the milk cream usually floats on the surface.

9. How do we collect blood serum for the test ?

Blood, taken from the animal, is collected in a tube, and is left in horizontal position for 30 minutes and then in vertical position for another 30 minutes, when the blood starts clotting. A clear liquid, called blood serum, then appears over the clot. Collect this serum in the dropper and pour 3-4 drops on the circular well of the test kit.

10.How do we interpret the test result ?

Positive : If two red lines appear it indicates the cow is pregnant or in middle period of estrous cycle.

Negative: If only one red line appears, it indicates that the cow is not pregnant.

Invalid: When control line does not appear on the membrane, the test is invalid due to improper test procedure or deterioration of reagents. It is recommended that the test be repeated

11. Test shows positive but afterwards the animal comes to heat again. What does it mean ?

Positive result means animal may be pregnant or is in luteal stage (middle period of estrus cycle). If it is in the middle period of estrus cycle then heat may come very shortly as in both the cases the level of progesterone hormone is high.