Assay Workflow

The CareDx AlloSeq cfDNA assay is a non-invasive, sample-to-report solution to measure transplant health. A single multiplex PCR is performed for each sample, in which locus-specific amplification of the targeted genes of interest and index PCR occur in the same reaction. Samples can then be pooled, purified and quantified to be ready for sequencing on an Illumina MiSeq instrument.