M D GroundsSchool of Anatomy and Human Biology, University of Western Australia, Crawley, WA, AustraliaCell Mol Life Sci 65:1621-5. 2008

..A deeper understanding of the events arising as an early consequence of dystrophin deficiency in these two situations may strengthen approaches to therapy for DMD designed to improve muscle function and the quality of life...

G M SmytheDepartment of Anatomy and Human Biology, The University of Western Australia, Nedlands, AustraliaCell Transplant 9:379-93. 2000

..The use of whole-muscle grafts provides an alternative and sensitive model to analyze the crucial effects of various tissue culture components on the subsequent survival and proliferation of myogenic cells in vivo...

The timing between skeletal muscle myoblast replication and fusion into myotubes, and the stability of regenerated dystrophic myofibres: an autoradiographic study in mdx mice

..These results have implications for understanding the cellular biology and pathology of dystrophic muscle, particularly in relation to myoblast transfer therapy as a potential treatment of Duchenne muscular dystrophy...

Superior survival and proliferation after transplantation of myoblasts obtained from adult mice compared with neonatal mice

Tracey F Lee-PullenDiscipline of Microbiology, School of Biomedical and Chemical Sciences, The University of Western Australia, QEII Medical Centre, Nedlands, Perth, WA 6009, AustraliaTransplantation 78:1172-6. 2004

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Enhanced migration and fusion of donor myoblasts in dystrophic and normal host muscle

G M SmytheDepartment of Anatomy, The University of Western Australia, Nedlands, Perth, Western Australia, 6907Muscle Nerve 23:560-74. 2000

..These data endorse the use of host T-cell depletion as a promising long-term strategy to improve myoblast transfer therapy (MTT) in the clinical situation...

A novel role for non-muscle gamma-actin in skeletal muscle sarcomere assembly

C M LloydSchool of Anatomy and Human Biology, The University of Western Australia, Crawley, Western Australia 6009, AustraliaExp Cell Res 297:82-96. 2004

..Consequently, a modified model is proposed which describes the role of gamma-actin in skeletal sarcomere assembly...

The role of tumor necrosis factor-alpha (TNF-alpha) in skeletal muscle regeneration. Studies in TNF-alpha(-/-) and TNF-alpha(-/-)/LT-alpha(-/-) mice

R A CollinsDepartment of Anatomy and Human Biology, The University of Western Australia, Nedlands, Western AustraliaJ Histochem Cytochem 49:989-1001. 2001

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A comparison between real-time quantitative PCR and DNA hybridization for quantitation of male DNA following myoblast transplantation

Erika BosioDiscipline of Microbiology, School of Biomedical and Chemical Sciences, The University of Western Australia, Perth, WA, AustraliaCell Transplant 13:817-21. 2004

..5 times higher than the maximum possible with slot-blot. In conclusion, Q-PCR has a higher dynamic range and is more efficient than slot-blot analysis for the detection of donor cell engraftment in a transsexual transplantation model...

Absence of MyoD increases donor myoblast migration into host muscle

G M SmytheDepartment of Anatomy and Human Biology, The University of Western Australia, Crawley, Perth, 6009, Western AustraliaExp Cell Res 267:267-74. 2001

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Complement and myoblast transfer therapy: donor myoblast survival is enhanced following depletion of host complement C3 using cobra venom factor, but not in the absence of C5

S I HodgettsDepartment of Anatomy and Human Biology, University of Western Australia, Crawley, Western Australia, AustraliaImmunol Cell Biol 79:231-9. 2001

..The observation that myoblast survival was increased in all mice treated with CVF suggests that CVF may indirectly enhance donor myoblast survival by a mechanism possibly involving activated C3 fragments...

Measurement of sub-membrane [Ca2+] in adult myofibers and cytosolic [Ca2+] in myotubes from normal and mdx mice using the Ca2+ indicator FFP-18

..This study suggests that the sub-sarcolemmal Ca(2+) homeostasis is well maintained in isolated adult mdx myofibers and also further supports the hypothesis that cytosolic Ca(2+) handling is compromised in mdx myotubes...

Innate inflammatory cells are not responsible for early death of donor myoblasts after myoblast transfer therapy

..These results support the hypothesis that p53 plays some role in TNF-mediated necrosis of dystrophic muscle and present a potential new target for therapeutic interventions...

Early regeneration of whole skeletal muscle grafts is unaffected by overexpression of IGF-1 in MLC/mIGF-1 transgenic mice

Thea ShavlakadzeSchool of Anatomy and Human Biology, University of Western Australia, Crawley, Western Australia 6009J Histochem Cytochem 52:873-83. 2004

..Skeletal muscle-specific overexpression of mIGF-1 does not appear to stimulate the early events associated with myogenesis during regeneration of whole muscle grafts...

Impact of fasting on the rhythmic expression of myogenic and metabolic factors in skeletal muscle of adult mice

T ShavlakadzeSchool of Anatomy, Physiology and Human Biology, The University of Western Australia, Crawley, Western Australia, AustraliaAm J Physiol Cell Physiol 305:C26-35. 2013

..This in vivo circadian study addresses for the first time the signaling activities of key molecules related to protein turnover and their possible cross-regulation of expression of genes related to protein degradation. ..

P W HamerDepartment of Human Movement and Exercise Science, The University of Western Australia, Crawley, AustraliaJ Anat 200:69-79. 2002

..This simple and inexpensive technique has sensitivity for the detection of increased myofibre permeability and/or sublethal damage that has advantages over other traditional histological techniques at the light microscopy level...

Thea ShavlakadzeM309, School of Anatomy and Human Biology, The University of Western Australia, Perth, Western AustraliaBiogerontology 11:363-76. 2010

..This delay is transient. Once inflammation occurs, myogenesis can proceed. The presence of well developed myotubes in old muscle autografts at 10 days confirms a very good inherent myogenic response of geriatric skeletal muscle...

A growth stimulus is needed for IGF-1 to induce skeletal muscle hypertrophy in vivo

Thea ShavlakadzeSchool of Anatomy and Human Biology, The University of Western Australia, Perth 6009, WA, AustraliaJ Cell Sci 123:960-71. 2010

..These data demonstrate that elevated IGF-1 has a hypertrophic effect on skeletal muscle only in growth situations...

The role of p53 in vivo during skeletal muscle post-natal development and regeneration: studies in p53 knockout mice

Jason D WhiteDepartment of Anatomy and Human Biology, The University of Western Australia, Crawley, AustraliaInt J Dev Biol 46:577-82. 2002

..These data indicate that p53 is not required for the regulation of myoblast proliferation, differentiation and myotube formation in vivo during myogenesis of adult skeletal muscle...

An evaluation of leukaemia inhibitory factor as a potential therapeutic agent in the treatment of muscle disease

..Serum protein levels of TNFalpha, IL-1beta, IL-6 and IL-12 fluctuated following irradiation treatments. These combined results strongly reinforce a major role for host immune cells in the rapid death of injected cultured donor myoblasts...

Thea ShavlakadzeSchool of Anatomy and Human Biology, The University of Western Australia, Crawley, Perth, Western Australia 6009, AustraliaGrowth Horm IGF Res 15:4-18. 2005

..Full understanding of these complex in vivo issues is essential, not only for skeletal muscle but for many other tissues, in order to effectively extend observations derived from transgenic studies into potential clinical situations...

A role for natural killer cells in the rapid death of cultured donor myoblasts after transplantation

Stuart I HodgettsSchool of Anatomy and Human Biology, The University of Western Australia, Crawley, Western AustraliaTransplantation 75:863-71. 2003

..These preliminary findings suggest that, with further development, 3D-OCT could be used as a tool for the evaluation of small-animal muscle morphology and pathology, in particular, for analysis of mouse models of muscular dystrophy...

Blockade of TNF in vivo using cV1q antibody reduces contractile dysfunction of skeletal muscle in response to eccentric exercise in dystrophic mdx and normal mice

A T PiersSchool of Biomedical, Biomolecular and Chemical Sciences, The University of Western Australia, Crawley, WA 6009, AustraliaNeuromuscul Disord 21:132-41. 2011

..This in vivo evidence that cV1q reduces the TNF-mediated adverse response to exercise-induced muscle damage supports the use of targeted anti-TNF treatments to reduce the severity of the functional deficit and dystropathology in DMD...

In vitro assessment of the biological activity of basic fibroblast growth factor released from various polymers and biomatrices

M D GroundsDepartment of Pathology, University of Western Australia, NedlandsMuscle Nerve 15:580-6. 1992

..Little difference in the regenerative capacity, either histologically or with respect to the timing of muscle precursor replication, was found between mdx mice and C57Bl/10Sn or other nondystrophic strains of mice...

..The results demonstrate that IGF-I can ameliorate fundamental aspects of E-C coupling failure in dystrophic muscle fibers and that these effects are important for the improvements in cellular function induced by this growth factor...

Dominic C H NgBiochemistry and Molecular Biology, School of Biomedical, Biomolecular and Chemical Sciences, University of Western Australia, Western Australia, AustraliaCell Motil Cytoskeleton 65:40-58. 2008

..Sarcomere formation in cardiac myocytes therefore, does not appear to require an intact MT network and thus we conclude that a functional MT array appears to be dispensable for myofibrillogenesis...

Analysis of the callipyge phenotype through skeletal muscle development; association of Dlk1 with muscle precursor cells