Methods: :
Post-mortem corneas were dissected from 10 donor globes - andwere preserved in Optisol-GS (B&L, Rochester, NY). The corneaswere then mounted on a microkeratome system (Moria, France)and were sectioned using a 300 micron head (119 to 182 hourspost harvest time, 152 ± 22 hours). After resection andwhile still mounted on the artificial anterior chamber, thecentral thickness of each cornea was measured using a conventionalUS pachymeter (Advent Pachymeter, Accutome, PA). Each Descemet-StrippingAutomated Endothelial Keratoplasty (DSAEK) section was thentransferred into a vial containing Optisol-GS at room temperatureand the vial was positioned in front of the Visante OCT usinga special adaptor (Abeamed, Miami FL) and the central thicknessof the DSAEK section was measured. The measured thicknessesobtained with OCT and US were compared.

Conclusions: :
Non-contact Visante OCT yields slightly larger measurementsof DSAEK donor thickness than US pachymetry, which requiredpressure on the tissue to obtain a measurement; however, thetwo systems are significantly correlated. OCT cannot contaminatetissues and therefore has this advantage over US pachymetry.