Overlay histogram showing HeLa cells stained with ab55275 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55275, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-IL13 receptor alpha 2 antibody (ab55275)

This product has been referenced in:

Pollack IF et al.Antigen-specific immunoreactivity and clinical outcome following vaccination with glioma-associated antigen peptides in children with recurrent high-grade gliomas: results of a pilot study.J Neurooncol130:517-527 (2016).
Read more (PubMed: 27624914) »

Thank you for contacting us. The molecular weight of Anti-IL13 receptor alpha 2 antibody (ab55275) is around 150KDa. I hope this helps and if we can assist further, please do not hesitate to contact us.