2019-02-14 11:00:002019-02-14 12:00:00Europe/ParisMicroOmiX : Enabling droplet microfluidics technologiesSINGLE CELL PROTEOMICS TO SINGLE CELL SECRETOMICS Droplet-based microfluidics allow the reliable production of micrometer-sized incubators in which individual cells can be isolated and manipulated at high throughput rates. Such microfluidics-based technologies have been […]Institut Pasteur, Rue du Docteur Roux, Paris, FrancePierre-Henri Commerepierre-henri.commere@pasteur.fr

Conférenciers

Sylvain Ursuegi

(faculté de Pharmacie Illkirch )

Présentation

SINGLE CELL PROTEOMICS TO SINGLE CELL SECRETOMICS

Droplet-based microfluidics allow the reliable production of micrometer-sized incubators in which individual cells can be isolated and manipulated at high
throughput rates. Such microfluidics-based technologies have been successfully interfaced with RNA-Seq enabling the analysis of cell heterogeneity at the
transcriptome level. However, those technologies still suffer from serious limitations linked to the lack of efficient method for in-droplet biomarker capture and purification preventing the possibility to perform multi-omic investigations (transcriptomic/proteomic).
MicroOmiX addresses these limitations by combining two proprietary technologies:

a highly efficient and robust capture system based on functional surfactant
allowing the scavenging of a wide range of biomarkers;

a droplet washing technology based on an inversion process enabling the
separation of captured biomarkers from contaminants and other cell remnants

The detection of purified biomarkers can then be performed using current analyzer:

a cytometer for a targeted approach (up to 30 RNA/protein biomarkers) or

a sequencer for a non-targeted transcriptomic strategy combined with targeted proteomic.