The task is made harder by a host of injuries with Joleon Lescott and James Milner the latest additions to a casualty list that includes Micah Richards, David Silva, Maicon and Jack Rodwell.

But manager Mancini said: "We have this problem with some important players injured but when you have this problem, it is a time that all the players can put more on the pitch.

"This is the last chance for us."

Mancini faced criticism over his tactics after the loss in Holland and he is struggling to improve his underwhelming overall record in the competition.

The former Inter Milan boss has never taken a team beyond the quarter-finals but he insists there is no secret to managing in the Champions League.

He just feels his current team are collectively inexperienced at this level.

The 47-year-old said: "For me, it is the same, but if I said we are ready to win the Champions League, this is not true. We need to improve.

"Probably there are other teams better than us in this competition because they have played every year in the Champions League."

Midfielder Gareth Barry insists the team retain the belief they can not only reach the knockout stages, but even win the competition.

The England international, 31, said: "As a group of players we are going into this game, believing we can win them and we can win the Champions League."

Despite that, Barry accepts that Europe's teams have found effective ways of combating the Barclays Premier League champions.

He said: "It is quite clear that we have not produced our best performances against the quality of opposition that are in the Champions League and, when it comes to these games, when you're not producing your best you get found out. That seems to be what has happened."

Mancini took unkindly to further questions about his future in the build-up to the game.

Last week the Italian neither confirmed nor denied reports he spoke to Monaco last season and he reacted angrily when asked for clarification at his pre-match press conference.

Mancini, who signed a five-year contract over the summer, said: "I don't understand why you continue to ask me [about] last year, last month. This is finished.

"Why? Why, for which reason? Why do you continue to ask me, 'Last year you had a chance to go'? I stay here because my work is here."