fungal storage

No it's not silly - I've found it a little frustrating. About 10 years ago our
mycologist tried lyopholysing all our fungal stocks - a couple of hundred. It
was a lot of effort that apparently went to waste. None of the hyphal fungi
ever grew from the vials. I don't know if it was a failure of the system, but
the Actino's and Nocardia's did well even after 10 yrs.
I found the sterile water and the sterile oil to be equally good. Of course it
does help to sub culture them once in a while.
>HI
> This may sound silly. But can anyone, tell me a good method for long
>storage of fungal cultures like they do for bacteria by glycerol storage.
> secondly, a good protocol for fungal staining and visualisation under
>a
>light microscrope.
> thanking you in advance
>cheers
>jayakumar
John Gentile M(ASCP) President - Rhode Island Apple Group
yjgent at aol.com
Microbiologists do it with culture.