Teaching a microbe to make fuel

August 20, 2012
by David Chandler

Micrograph shows a group of Ralstonia eutropha bacteria in culture. In their natural form, as seen here, the microbes convert carbon in their surrounding into a kind of bioplastic — seen as the light-colored dots inside their membranes. But a biologically engineered version developed at MIT instead produces isobutanol fuel, which is then expelled from the cells into the surrounding medium, where it can easily be collected. Image courtesy of Christopher Brigham

(Phys.org) -- A humble soil bacterium called Ralstonia eutropha has a natural tendency, whenever it is stressed, to stop growing and put all its energy into making complex carbon compounds. Now scientists at MIT have taught this microbe a new trick: They've tinkered with its genes to persuade it to make fuel  specifically, a kind of alcohol called isobutanol that can be directly substituted for, or blended with, gasoline.

Christopher Brigham, a research scientist in MIT's biology department who has been working to develop this bioengineered bacterium, is currently trying to get the organism to use a stream of carbon dioxide as its source of carbon, so that it could be used to make fuel out of emissions. Brigham is co-author of a paper on this research published this month in the journal Applied Microbiology and Biotechnology.

Brigham explains that in its natural state, when the microbe's source of essential nutrients (such as nitrate or phosphate) is restricted, "it will go into carbon-storage mode," essentially storing away food for later use when it senses that resources are limited.

"What it does is take whatever carbon is available, and stores it in the form of a polymer, which is similar in its properties to a lot of petroleum-based plastics," Brigham says. By knocking out a few genes, inserting a gene from another organism, and tinkering with the expression of other genes, Brigham and his colleagues were able to redirect the microbe to make fuel instead of plastic.

While the team is focusing on getting the microbe to use CO2 as a carbon source, with slightly different modifications the same microbe could also potentially turn almost any source of carbon, including agricultural waste or municipal waste, into useful fuel. In the laboratory, the microbes have been using fructose, a sugar, as their carbon source.

At this point, the MIT team  which includes chemistry graduate student Jingnan Lu, biology postdoc Claudia Gai, and is led by Anthony Sinskey, professor of biology  have demonstrated success in modifying the microbe's genes so that it converts carbon into isobutanol in an ongoing process.

"We've shown that, in continuous culture, we can get substantial amounts of isobutanol," Brigham says. Now, the researchers are focusing on finding ways to optimize the system to increase the rate of production and to design bioreactors to scale the process up to industrial levels.

Unlike some bioengineered systems in which microbes produce a wanted chemical within their bodies but have to be destroyed to retrieve the product, R. eutropha naturally expels the isobutanol into the surrounding fluid, where it can be continuously filtered out without stopping the production process, Brigham says. "We didn't have to add a transport system to get it out of the cell," he says.

A number of research groups are pursuing isobutanol production through various pathways, including other genetically modified organisms; at least two companies are already gearing up to produce it as a fuel, fuel additive or a feedstock for chemical production. Unlike some proposed biofuels, isobutanol can be used in current engines with little or no modification, and has already been used in some racing cars.

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2 comments

Direct production of fuels that need minimal "refinement" is the future of hydrocarbon energy carriers. Production for both higher vapor pressure fuels like isobutanol and lower ones like biodiesel will be the target of many patents by the large energy companies. This will probably drive an increase in the number of processes developed for the production of the same set of fuels all vying for big energy purchase.

Fructose is in a 'high energy state' which organisms can tap to power reactions within cells. Another way to put it is, fructose is 'food' to a soil bacterium.

Carbon dioxide, by contrast, is in a very low energy state. It's not useful for powering cellular chemistry. It's not 'food.'

Thus the leap from fructose to CO2 as feedstock for producing alcohol waste from a soil bacterium is not as trivial as the authors seem to imply. Chemically, the bacterium will have to convert low energy molecules into high energy molecules. It will need much more food energy to do that than it will need to convert fructose to alcohol.

I wonder why they aren't looking at photosynthesizing bacteria?

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