Hi all,
Several colleagues of mine have advised me against using high
concentrations (8 M) of urea in ion exchange runs. In their
experience, nothing binds under this conditions. This puzzles me,
because being urea an uncharged species, it shouldn't interfere with
ion exchange, right?
Does anybody have similar experiences and/or have an explanation?
Cheers,
alejandro
Alejandro Martin
Division of Vaccines,
CIGB
PO Box 6162
La Habana, Cuba
CP 10600