Application Information: p38 gamma antibody [10E1]

WB 1:500 IHC 1:50 Flow 1:500Optimal working dilutions should be determined experimentally by the end user.

Form Supplied

Liquid

Purification

Protein A/G purified

Specifications: p38 gamma antibody [10E1]

Full Name

mitogen-activated protein kinase 12

Background

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation.

Storage Conditions: p38 gamma antibody [10E1]

For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles.

Notes

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

p38 gamma antibody [10E1] validated data

GTX84148 FACS Image

HEK293T cells transfected with either MAPK12 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPK12 antibody (GTX84148), and then analyzed by flow cytometry.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK12 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK12.

HEK293T cells transfected with either MAPK12 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPK12 antibody (GTX84148), and then analyzed by flow cytometry.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK12 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK12.