Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Applications

Specificity and Use

Recognizes defined epitope (aa 16-25) on human p53 (tumor suppressor p53), a 50 kD tumor antigen found in increased amounts in a wide variety of transformed cells; it is frequently mutated or inactivated in many types of cancer.

Usage:

The reagent is designed for direct immunofluorescence analysis. Note: This conjugate is routinely tested by flow cytometry analysis using permeabilized transient wild-type p53-tranfectants. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Description: Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases.