One Hand, One Heart from West Side Story is featured in The First Time, the fifth episode of Season Three. It is sung in the school play, West Side Story, by Blaine and Rachel as Tony and Maria.

During the song, there are scenes that show Finn and Rachel laying together in front of a fire along with Blaine and Kurt lying on Blaine's bed. At the end, Finn and Kurt are seen watching the performance, proud of their partners.