Custom antibody development

We offer world-leading antibody development platforms based on a proprietary RabMAb® rabbit monoclonal platform, a phase display platform (AxioMx), and a next-generation sequencing platform (NGS-RabMAb®)

Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

IHC-P

Use a concentration of 10 µg/ml.

ELISA

Use at an assay dependent concentration.

RIA

Use at an assay dependent concentration.

WB

Use at an assay dependent concentration.

Works best under non reducing conditions.

Sandwich ELISA

Use a concentration of 1 µg/ml. For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab9538 as Detection.

ICC

Use at an assay dependent concentration.

IP

Use at an assay dependent concentration.

Functional Studies

Use at an assay dependent concentration. Effectively blocks the transferrin-transferrin receptor interactions.

Target

Function

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.

Tissue specificity

Expressed by the liver and secreted in plasma.

Involvement in disease

Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.

Lateral flow is not a tested application, however it is very much like sandwich ELISA so any ab pair used in sELISA should work in LF. We do offer a matched antibody for use with ab769 this is ab9538.
Gold conjugation is generally used in LF. We d...

Thank you for the requested information. I have re-examined our record and I was not able to find this information in our past correspondences with you.
In regards to the purchase order number "192677", this was an order for ab7355 (Acinus antibody...