Applications:
For use in the standardization of forensic and paternity quality assurance procedures for polymerase chain reaction (PCR)-based genetic testing, for instructional law enforcement or non-clinical research purposes, and for quality assurance when assigning values to in-house control materials. It is not intended for any human or animal clinical diagnostic use.

SRM NIST-2391c is composed of well-characterized human deoxyribonucleic acid (DNA) in two forms: genomic DNA (Components A through D) and DNA to be extracted from cells that have been spotted onto 903 paper (Component E) and FTA paper (Component F).

Component

Description

Amount

Concentration(a)

A

Anonymous single-source female genomic DNA in TE-4 buffer

50 μL

1.1 – 2.1 ng/μL

B

Anonymous single-source male genomic DNA in TE-4 buffer

50 μL

1.1 – 2.1 ng/μL

C

Anonymous single-source male genomic DNA in TE-4 buffer

50 μL

1.1 – 2.1 ng/μL

D

Mixed-source (Components A and C) genomic DNA in TE-4 buffer

50 μL

1.1 – 2.1 ng/μL

E

Anonymous single-source female cells spotted on 903 paper

Two 6 mm punches

7.5 × 104 cells per punch

F

Anonymous single-source male cells spotted on FTA paper

Two 6 mm punches

7.5 × 104 cells per punch

See the NIST Certificate of Analysis for directions for use and quality control specifications.

SRM NIST-2372 is intended primarily for use in the value assignment of human genomic DN forensic quantitation materials. It is not intended for any human or animal clinical diagnostic use. SRM 2372 consists of three well-characterized human genomic DNA materials solubilized in 10 mmol/L 2-amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride (Tris HCl) and 0.1 mmol/L ethylenediaminetetraacetic acid disodium salt (disodium EDTA) using deionized water adjusted to pH 8.0 buffer (TE-4). The three component genomic DNA materials, labeled A, B, and C, are derived from a single male donor, multiple female donors, and multiple male and female donors, respectively. A unit of the SRM consists of one sterile 2 mL vial of each component, each vial containing approximately 110 μL of DNA solution. Each of these vials is labeled and is sealed with a color–coded screw cap.

See the NIST Certificate of Analysis for directions for use and quality control specifications.