STEDYCON

Abberior Instruments is known for extraordinary customization ability of its flexible STED microscope platform. We have developed the ultimate compact STED plus confocal microscope, called STEDYCON.

STEDYCON is a completely new class of nanoscope. It converts your conventional epifluorescence microscope into a powerful multicolor confocal (405nm, 488nm, 561nm, 640nm) and STED (775nm) system! At the same time, it is incredibly compact and can be used by anyone!

Save Space - The incredibly compact STEDYCON comes at the size of a camera.

Plug & Play - The STEDYCON is assembled within minutes. It simply connects to the C-mount camera port of your microscope with no need for optical alignment.

Service and maintenance free - The laser beams of the STEDYCON are aligned by design as provided by its patent-protected 'easySTED' optical arrangement.

Innovative web-based user interface - The STEDYCON can be controlled via a browser on any PC, MAC or even a tablet!

Our STEDYCON nanoscope works with all kinds of microscope bodies from all major vendors:

STEDYCON @ Leica DM2500

STEDYCON @ Leica DMI6000

STEDYCON @ Nikon NiE

STEDYCON @ Nikon Ti2

STEDYCON @ Nikon TiE

STEDYCON @ Olympus BX53

STEDYCON @ Olympus IX83

STEDYCON @ Zeiss Axio Imager

STEDYCON @ Zeiss Axio Observer with AFM

STEDYCON @ Zeiss Axio Observer Z1

STEDYCON @ Zeiss Axio Vert

Browser-based cross-platform software

The STEDYCON can be controlled via Abberior Instruments STEDYCON smart control, a web-browser-based graphical user interface. STEDYCON smart control was designed from scratch to provide the highest possible usability completing the overall STEDYCON concept of easy installation, intuitive usage, and maintenance-free operation. Beginners can image with super-resolution within minutes. Smart control runs on every device with a modern web browser like Windows-based PCs, Linux-based PCs, Apple PCs or even tablets.

Gattaquant STED-AFM ruler (70 nm distance between emitters) were imaged using a STEDYCON attached to a Nikon Eclipse Ni and a Zeiss Axio Imager Z2. Shown is RAW DATA

Two proteins in the Golgi apparatus were immunolabelled using primary antibodies specific for GM130 and Giantin and secondary antibodies coupled to Abberior STAR 580 and Abberior STAR 635P. Shown is RAW DATA. Images were acquired using a STEDYCON attached to a Zeiss Axio Imager Z2.

The nuclear lamina and nuclear pore complex of mammalian cells were immunolabelled using Abberior STAR 580 and Abberior STAR 635P. Shown is RAW DATA. Images were acquired using a STEDYCON attached to a Zeiss Axio Imager Z2.

Two subunits of the nuclear pore complex were immunolabelled using antibodies against gp210 and antibodies with multiple specificities (PAN4/5) and secondary antibodies coupled to Abberior STAR580 and Abberior STAR635P. Note that gp210 is localized in an eightfold symmetric structure at the rim of the nuclear pore complex. Shown is RAW DATA. Images were acquired using a STEDYCON attached to a Zeiss Axio Imager Z2.

Two proteins in the Golgi apparatus were immunolabelled using primary antibodies specific for GM130 and Giantin and secondary antibodies coupled to Abberior STAR 580 and Abberior STAR635P. Shown is a close-up.

The tubulin and vimentin cytoskeleton of mammalian cells were immunolabelled using Abberior STAR 580 and Abberior STAR 635P. Shown is RAW DATA. Images were acquired using a STEDYCON attached to a Nikon Eclipse Ni.

Two proteins in the Golgi apparatus were immunolabelled using primary antibodies specific for GM130 and Giantin and secondary antibodies coupled to Abberior STAR 580 and Abberior STAR 635P. Shown is RAW DATA. Images were acquired using a STEDYCON attached to a Nikon Eclipse Ni.

Two subunits of the nuclear pore complex were immunolabelled using antibodies against gp210 and antibodies with multiple specificities (PAN4/5) and secondary antibodies coupled to Abberior STAR580 and Abberior STAR635P. Note that gp210 is localized in an eightfold symmetric structure at the rim of the nuclear pore complex. Shown is RAW DATA. Images were acquired using a STEDYCON attached to a Nikon Eclipse Ni.

Software: browser-based control software operational on any modern PC, Mac or tablet: line interleaved STED and confocal imaging in three imaging channels; options to define z-stacks, acquisition queues and time series, auto-save function