I am doing some DNA electrophoresis from marine yeast using the following
restriction enzymes: Alu I, Eco RI, Hinf I, Kpn I, Pst I. I am using 2%
agarose gel. At least in Alu I and Eco RI I can see some patterns.
However, the background is so intense that I can't distinguish very well
the pattern. The smear is lightly less intens than the bands.
Is it because I have both DNAs (genomic and mitochondrial)?
How can I have more clear gels in a way to take nice pictures?
I am really concerned about this because my thesis is almost finished
but I can't have nice imagens from this gels. If you have some ideas
about what to do please e-mail me to:
asanchez at cibnor.mx
Arturo Sánchez
Marine Pathology Unit.
Marine Yeast Laboratory.
Center for Biological Research.
La Paz, Baja California Sur. México.