Abstract:A capillary electrophoretic method (CE) with ultraviolet absorption
detection for baclofen in human plasma was developed. The separation was
obtained by CE with 50 mM
borate buffer at pH 9, followed by detection with an ultraviolet detector at
225 nm. The plasma samples were deproteinized with acetonitrile to remove
proteins and to induce sample stacking. With stacking, the limit of detection
(LOD) for baclofen in plasma was 0.2 mM. A calibration curve ranging from 1.5 to 300 mM showed to be linear. Both the within-day and day-to-day
reproducibility and accuracy were less than 15% and 8%, respectively. The
recoveries of baclofen in human plasma were 99.6 –
103.3%.