アプリケーション

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション

Abreviews

特記事項

WB

1/500. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).

IP

1/100.

IHC-P

Use at an assay dependent concentration.

ICC/IF

1/250 - 1/500.

Flow Cyt

1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

機能

Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.

関連疾患

Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.

配列類似性

Belongs to the integrin beta chain family.Contains 1 VWFA domain.

翻訳後修飾

Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.

Anti-CD18 (phospho S745) antibody [EP1288Y] 画像

ICC/IF image of ab52920 stained Raw 246.7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab52920 at 1/1000 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has...

For ab63388 you get 100 µg at 1mg/ml. For ab52920 you get 100ul butthe concentration varies slightly from batch to batch and so if you could let me know the lot number that you currently have, I will be h...

Thank you for contacting us. ab63388 hasn't been tested in WB so we can’t confirm the suitability of this antibody due to lack of tested data. However this does not mean this cannot be used in WB this only means we have not tested it. If you are ...

Thank you for contacting us. Could you let us know the phosphorylated amino acid you are interested in? The integrin beta 2 shows phosphorylation at Serine 745, 756 and at Threonine 758, 759 and 760. The antibodies we have are ab52920 and ab63388 I hop...