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enCRAll About PCR22
Polymerase: Enzyme that synthesizes a DNA strand from a template
Thermocycler: Equipment that goes through repeated cycles of temperature changes
Primers: Short oligonucleotides used as the starting point for DNA synthesis
Template: Target DNA to be amplified
Taq: Polymerase isolated from the bacterium Thermus aquaticus
dNTPs: building blocks for DNA synthesis
Denaturation: Temperature-mediated separation of the DNA double helix
Annealing: Stage in which the primers bind to the DNA target
Reverse Transcriptase: Enzyme responsible for transcribing RNA into cDNA (2 wds)
RTPCR: Amplification of DNA from RNA
Quantitative: q, in qPCR
Mullis: PCR inventor
Nested: A variation of the PCR technique in which two sets of primers are used successively
Exonuclease: High-fidelity polymerases possess 3’ to 5’ __________ activity.
Tm: Primer GC content often increases this
Hairpins: Dimers and these can result in reduced primer efficiency
Magnesium: Cofactor ions used in PCR reactions
Hot start: PCR variation that involves heating the reaction before adding the polymerase (2 wds)
Reaction: Polymerase chain _____________
Pfu: Proof-reading polymerase isolated from Pyrococcus furiosus
Amplicon: DNA product generated by PCR
Agarose gel: Following PCR, the quality of the product is often assessed by this (2 wds)
Fluorescent: ________ dyes, such as SybrGreen, are often used to determine the amount of product in real time.

All About PCR

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Across:

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q, in qPCR

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Stage in which the primers bind to the DNA target

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PCR inventor

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Dimers and these can result in reduced primer efficiency

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PCR variation that involves heating the reaction before adding the polymerase (2 wds)

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Short oligonucleotides used as the starting point for DNA synthesis

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Target DNA to be amplified

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Enzyme that synthesizes a DNA strand from a template

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Proof-reading polymerase isolated from Pyrococcus furiosus

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Primer GC content often increases this

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Polymerase chain _____________

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A variation of the PCR technique in which two sets of primers are used successively

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High-fidelity polymerases possess 3’ to 5’ __________ activity.

Down:

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Following PCR, the quality of the product is often assessed by this (2 wds)

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Equipment that goes through repeated cycles of temperature changes

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________ dyes, such as SybrGreen, are often used to determine the amount of product in real time.