Community Commentary

In a BY4735 background, knocking out YJR061W seems to have little to no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 414 minutes, while the YJR061W knock-out strain's doubling time was 436 minutes. (These doubling times are the means of three experiments.)

Caffeine and Yeast Cells

This graph shows the growth curve with and without caffeine. It has the knockout strain and wild type yeast cells. The caffeine enhanced the growth in both wild type and knockout strain. The average doubling time between the three experiments for the knockout strand was 824.60 minutes. The caffeine also had a positive effect on the growth curve for the wild type with the average doubling time being 1245.02 minutes. The results were concluded from the wild type and knockout strand was with this amount of caffeine it leads to an increase in growth.

Cycloheximide effecting YJR061W

As you can see in the figure above test 4 was thriving and doing well. Tests 1, 2, & 3 were below the growth curve. Growth models 1-3 are The yeast strand YJR061 without any stress.

Effect of 3% DMSO on YJR061W

The graph above shows the effect of 3% DMSO on YJR061W. From the data represented the average doubling rate for the YJR061W knockout strain without DMSO was 591 minutes. When DMSO is added the average doubling time increased to 1331 minutes. This shows that 3% DMSO did add stress to this knockout strain. The average doubling time of WT:BY4735 without DMSO was 514 minutes. The average doubling rate for WT:BY4735 with DMSO was 1529 minutes. If we compare the average doubling rate of WT:BY4735 with DMSO to YJR061W with DMSO we can see that without the YJR061W gene the yeast was able to grow more quickly under DMSO induced stress. It is also observed that both control groups had a very close growth rate, which means that the YJR061W gene has a minimal effect of yeast growth in optimal conditions.

Ethanol Tolerance in YJR061W

This graph shows the growth between YJR061W treated and untreated with ethanol. The yeast treated with ethanol had a growth rate of (answer). The yeast not treated with ethanol had a growth rate of (answer). This shows that the strands of YJR061W treated with ethanol had an increased growth rate compared to those who weren't.