Yeast Plasmid Isolation Kit, Yeast Plasmid Mini-Preps

The Easy Yeast Plasmid Isolation Kit provides a simple and efficient plasmid mini-prep method for rescuing plasmid DNA from yeast (Saccharomyces cerevisiae). The protocol uses Zymolyase for highly efficient enzymatic disruption of yeast cell walls, followed by SDS/alkaline lysis of the resulting spheroplasts. A spin column is used to purify the plasmid DNA, which can then be transformed into E. coli for propagation and scaled-up plasmid preparations, or used as a template for PCR.

Features

Applications

Plasmid DNA mini-preps from yeast

Rescue positive prey plasmids after a yeast two-hybrid or a yeast one-hybrid screen

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.