Get closer to the real Host Cell Protein (HCP) quantity

Recombinant protein production and protein purification raises the tricky question of host cell proteins (HCPs). To monitor the purification level of your protein of interest, the HCPs must be quantified. Of course, the quality and the optimisation of purification depends upon it. Here, we’ll take a look at an alternative to the gold standard, offering a way to detect more HCPs present in the production. So, are you ready for higher grade detection?

How about 25% more coverage?

The Canopy process of affinity purification of antibodies is very different from the leading competitor, giving us more sensitive antibodies that recognize a wider range of host cell proteins.

Canopy’s CHO HCP antibody was tested under similar conditions as competitor “C’s” CHO HCP antibody using 2D western blot analysis.

2-8 fold more HCPs

The amount of HCP present in 3 different DSs (therapeutic antibodies, produced by CHO) was assessed by both the Canopy CHO HCP kit and the Competitor “C” kit. Canopy’s antibody detected 2-8 fold more HCPs. Fas, S. BioPharmaceutical Emerging Best Practices Association. 2014