JAPSJournal of Applied Pharmaceutical Science2231-3354Open Science Publishers LLP10.7324/JAPS.2016.60314<TEXTFORMAT LEADING="2"><P ALIGN="LEFT"><FONT FACE="Arial" SIZE="11" COLOR="#000000" LETTERSPACING="0" KERNING="0">Toxicological Study of a Siddha Sastric Formulation Arumuga Chendhuram in Rat Model</FONT></P></TEXTFORMAT>Ramamurthy MuruganThanigavelan VembuManickavasakam Kumarswamy630The study was aimed to profile the acute and sub-acute oral toxicity of a herbo-metallic drug Arumuga Chendhuram (AC). AC was prepared classically and analyzed for elemental composition using X-ray Fluorescence. Acute oral toxicity study was done on female rats at AC 2 g/kg as single administration following OECD guideline 423. For sub acute toxicity study, AC was administered orally for 28 consecutive days suspended in vehicle (Honey + distilled water) to rats following OECD guideline 407. Four groups was allotted (10 rats/group), control received vehicle; the other received AC at 12, 24 and 48 mg/kg/day respectively. Mortality and abnormal clinical signs were observed. Haematological and biochemical parameters were analyzed using auto analyzer with standard kits and ANOVA-Dunnett test was performed for significant analyses. Gross necropsy and histopathology studies using HandE stain were done on major organs. Mercury and Lead were found more than the WHO permissible limits in XRF study. LD50 was found more than 2 g/kg. No-Observed-Adverse-Effect level of AC was seen at 24 mg/kg in 28 days of treatment. No abnormal findings were noted in high dose group organs. Administration of AC at its human therapeutic dose of 260 mg/kg in rat (24 mg/kg) is safe.keywordsSiddhaChendhuramAcute ToxicitySubacute ToxicityArumuga Chendhuram.3162References