ResultsWe show that myosin ii motors and F-actin dynamics are required for Golgi apparatus positioning before nucleokinesis in cerebellar granule neurons CGNs migrating along glial fibers. Moreover, we show that primary cilia are motile organelles, localized to the leading-process F-actin-rich domain and immobilized by pharmacological inhibition of myosin ii and F-actin dynamics. Finally, leading process adhesion dynamics are dependent on myosin ii and F-actin.

ConclusionsWe propose that actomyosin coordinates the overall polarity of migrating CGNs by controlling asymmetric organelle positioning and cell-cell contacts as these cells move along their glial guides.

AbbreviationsCGNCerebellar granule neuron

FRAPFluorescence recovery after photobleaching

KO2Kusibira orange

MGEMedial ganglionic eminence

RFPRed fluorescent protein

TFPTeal fluorescent protein

UTRCH-ABDUtrophin actin-binding domain

YFPYellow fluorescent protein.

Electronic supplementary materialThe online version of this article doi:10.1186-1749-8104-9-26 contains supplementary material, which is available to authorized users.