About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Antibody's recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.

Tested applications
To be tested

Antibody's datasheet
Contact our support service to receive datasheet or other technical documentation.

Total weight (kg)
1.4

Stock availabilit
In Stock

Scientific context
PERK (eukaryotic translation initiation factor 2-α kinase 3, eIF2α kinase 3, PEK, WRS) is a Ser/Thr protein kinase, type I membrane protein of the endoplasmic reticulum that is abundant in secretory tissues and is inducible by ER-stress. PERK phosphorylates the α subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, a reduction of translation initiation and repression of global protein synthesis. PERK may serve as an effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1. PERK dimerizes with HSPA5/BIP in resting cells and oligomerizes in ER-stressed cells. Perturbation in protein folding in the endoplasmic reticulum (ER) can promote dissociation from HSPA5/BIP and oligomerization, resulting in autophosphorylation and kinase activity induction.

Antibody's target
Human PERK (pThr892)

Certificate of analysis
A 1:250 dilution of SPC-963 was sufficient for detection of PERK (pThr892) in 10 µg of HepG2 cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Tissue specificity
See included datasheet or contact our support service.