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Human p53/tumor protein(P53) ELISA Kit

The kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) to assay the level of Human p53/tumor protein(P53) in samples. Add p53/tumor protein(P53) to monoclonal antibody Enzyme well which is pre-coated with Human p53/tumor protein (P53)monoclonal antibody, incubation then, add p53/tumor protein(P53)antibodies labeled with biotin, and combined with Streptavidin-HRP to form immune complex then carry out incubation and washing again to remove the uncombined enzyme. Then add Chromogen Solution A, B, the color of the liquid changes into the blue, And at the effect of acid, the color finally becomes yellow. The chroma of color and the concentrations of the Human Substance p53/tumor protein(P53) of sample were positively correlated.