Hi,
I=92ve been trying to isolate RNA and DNA from the same sample (tissue cult=
ure fluid spiked with viruses) and, while RNA isolation seems to be working=
fine, I don=92t recover any viral DNA that I originally put into the sampl=
e. According to the protocol supplied with Trizol (Invitrogen), DNA should =
be precipitated by ethanol and pelleted by centrifugation at 2,000 g for 5 =
minutes, following several washes with the same centrifugation speed to rec=
over the DNA pellet. The recommended speed of the centrifugation seems very=
low, so if those of you who use Trizol could tell me whether or not they s=
tick to the recommended protocol, I would be grateful=85
Thanks!
Magda
Magda Dunowska, LW, PhD
Senior Lecturer in Veterinary Infectious Diseases (Virology)
Institute of Veterinary, Animal and Biomedical Sciences
Te Kura M=E2tauranga Kararehe
Massey University
Palmerston North
New Zealand
Phone : (06) 350-5799 ext 7571
Website : http://ivabs.massey.ac.nz<http://ivabs.massey.ac.nz/>