Abstract

Background: Nowadays, veterinary practice, involves treatment of disorders associated with cartilage degeneration using advanced therapy, based on mesenchymal stromal cells application. Preparation of cells for the transplantation requires in vitro culture and evaluation of their chondrogenic potential.

Results: The enhancement of proliferation activity was noticed in cultures stimulated with exogenous nucleotides. Although the addition of gelsolin did not affect EqASCs proliferation, it contributed to cytoskeleton integrity. Both investigated factors positively influenced on chondrogenic differentiation – gelsolin through stabilization of cytoskeleton proteins expression, while nucleotides by promoting nodules formation. Combination of gelsolin and nucleotides enhanced cells proliferation, what was reflected by higher cellular activity and microvesicles shedding and influenced on chondro nodules formation.