KEYWORDS:

Study design and timeline. Dotted vertical lines and “M” designates the windows in which metabolic monitoring was performed. The black arrow designates the end of postOVX calorically limited (CL) feeding at the start of postOVX ad libitum (AL) feeding.

Changes in energy balance, energy intake, and total energy expenditure (TEE) from before OVX to the early refeeding phase (A) and to the end of the study (B). P < 0.05, main effect of weight loss for all variables for both time points.

(A) Total spontaneous physical activity (SPA) at each time point. (B) Relative changes in total SPA from before OVX to the calorically limited phase (before regain) and early during the refeeding phase after OVX, and just prior to sacrifice. (C) Ambulatory spontaneous physical activity (SPA) at each time point. (D) Relative changes in ambulatory SPA from before OVX to the calorically limited (CL) phase (before regain) and early during the ad libitum (AL) refeeding phase after OVX, and just prior to sacrifice. No significant difference in change between groups.