Effects of camptothecin on the breakage and repair of DNA during the cell cyle

Abstract

Incubation of camptothecin with HeLa cells in either the G1 or S phase of the cell cycle results in fragmentation of DNA as analyzed on alkaline sucrose gradients. Removal of the drug from G1 or S phase cells results in the normal sedimentation of cellular DNA. Neither the fragmentation nor the repair of HeLa cell DNA requires DNA replication. However, subtle irreversible changes must take place in the DNA in cells treated with camptothecin for 30 min during the S phase, since such cells are unable to undergo subsequent cell division. The lethal effect is presumed to be at the single strand replication forks.

title = "Effects of camptothecin on the breakage and repair of DNA during the cell cyle",

abstract = "Incubation of camptothecin with HeLa cells in either the G1 or S phase of the cell cycle results in fragmentation of DNA as analyzed on alkaline sucrose gradients. Removal of the drug from G1 or S phase cells results in the normal sedimentation of cellular DNA. Neither the fragmentation nor the repair of HeLa cell DNA requires DNA replication. However, subtle irreversible changes must take place in the DNA in cells treated with camptothecin for 30 min during the S phase, since such cells are unable to undergo subsequent cell division. The lethal effect is presumed to be at the single strand replication forks.",

author = "{Band Horwitz}, Susan and Horwitz, {M. S.}",

year = "1973",

language = "English (US)",

volume = "33",

pages = "2834--2836",

journal = "Journal of Cancer Research",

issn = "0008-5472",

publisher = "American Association for Cancer Research Inc.",

number = "11",

}

TY - JOUR

T1 - Effects of camptothecin on the breakage and repair of DNA during the cell cyle

AU - Band Horwitz, Susan

AU - Horwitz, M. S.

PY - 1973

Y1 - 1973

N2 - Incubation of camptothecin with HeLa cells in either the G1 or S phase of the cell cycle results in fragmentation of DNA as analyzed on alkaline sucrose gradients. Removal of the drug from G1 or S phase cells results in the normal sedimentation of cellular DNA. Neither the fragmentation nor the repair of HeLa cell DNA requires DNA replication. However, subtle irreversible changes must take place in the DNA in cells treated with camptothecin for 30 min during the S phase, since such cells are unable to undergo subsequent cell division. The lethal effect is presumed to be at the single strand replication forks.

AB - Incubation of camptothecin with HeLa cells in either the G1 or S phase of the cell cycle results in fragmentation of DNA as analyzed on alkaline sucrose gradients. Removal of the drug from G1 or S phase cells results in the normal sedimentation of cellular DNA. Neither the fragmentation nor the repair of HeLa cell DNA requires DNA replication. However, subtle irreversible changes must take place in the DNA in cells treated with camptothecin for 30 min during the S phase, since such cells are unable to undergo subsequent cell division. The lethal effect is presumed to be at the single strand replication forks.