Asian Science
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Abstract: Substance P (SP) is known to play an important role in bone
mineralization. However, little is known about the potential of substance P
as an osteogenic inducer in human Adipose-Derived Stem Cells (hADSCs) in
vitro. Therefore, the aim of this study to investigate the effects of substance
P on proliferation and osteogenic differentiation of hADSCs in vitro.
Human Adipose-Derived Stem Cells (hADSCs) derived from wasted adipose tissue
in liposuction operation were cultured. Expression of the SP receptor (NK1)
was detected by RT-PCR. Effects of SP on proliferation and differentiation of
hADSCs were studied, moreover alkaline phosphatase activity was also assayed.
It was found that the NK1 receptor was expressed in hADSCs. SP stimulated the
proliferation of hADSCs in a concentration-dependent manner. Higher concentrations
(10G8 M) of SP increased alkaline phosphatase activity and enhanced
differentiated in hADSCs. NK1 receptors are expressed by hADSCs and SP stimulates
osteoblast differentiation and function in vitro. These findings strengthen
the knowledge about the role of SP in hADSCs.