Suitable for use in ELISA, Western Blot, Immunocytochemistry and Human T-Cell research. Other applications not tested.

Recommended Dilutions

ELISA: 1:1000

Western Blot: 1:1000

Optimcal dilutions to be determined by the researcher.

Molar Ratio

3-4 peroxidase molecules per molecule of antibody

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.