Dear Methodists and Reagentites-
With all of the vast resources and
experience out there in 'net'land, has anyone
come up with a way to cross link a protein
coupled with a HIS tag to a nickel [or
other column] for the purpose of affinity
purifying antibodies.
I do not want to activate CNBr-Sephadex
but exploit the property of a rapid purification
on one column. I am hoping that the interaction
between the His Tag and the nickel-NTA column
is stronger than the antigen-antibody interaction.
If anyone has experience or suggestions,
Please help me if you can.
Stephanie Greene
cleaner antibodies, for cleaner results