Positive Controls

Histology Controls

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References

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IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations. Western blot analysis against affinity purified ovine IgE using Mouse anti Sheep IgE clone 1E7 demonstrates a single major band of approximately 80kDa under reducing conditions, and a band at approximately 200 kDa is observed after electrophoresis under non-reducing conditions. These bands correspond with the expected molecular weights of the epsilon chain and the complete ovine IgE molecule (Kooyman, F.N. et al. 1997).

IgE is in important in both type 1 hypersensitivity and immunity to parasite infections, in particular parasitic worm infections. Clone 1E7 has been used in a number of studies involving parasite nematode infections of sheep by Haemonchus contortus (Vervelde, L. et al. 1997 and Kooyman, F.N. et al. 1997). Clone 1E7 also recognizes bovine IgE, and as such it is useful for studies of infection in cattle by nematode worms such as Dictyocaulus viviparus (Bricarello, P.A. et al. 2007 and Kooyman, F.N. et al. 2002).

In addition to this specific anti IgE monoclonal antibody, monoclonal antibodies specific to both bovine and ovine, IgA, IgM and IgG subclasses are available from Bio-Rad.

Intended Use

Target Species

Sheep

Species Cross-Reactivity

Target Species

Cross Reactivity

Bovine

Goat

Horse

Human

N.B. Antibody reactivity and working conditions may vary between species.

Store at +4oC or at -20oC if preferred.This product should be stored undiluted.Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

18 months from date of despatch

Acknowledgements

Regulatory

For research purposes only

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name

Yes

No

Min Dilution

Max Dilution

ELISA

1/500

1/2000

Immunofluorescence

Immunohistology - Frozen

Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.