How to order

Order standard gene synthesis online

Standard gene synthesis uses our highly automated process with turnaround times starting at just 9 business days, depending on sequence length (quicker options available). It is appropriate for most constructs with uncomplicated secondary structure and without direct/indirect repeats or long polypurine/polypyrimidine runs. Like all GeneArt Gene Synthesis services, standard gene synthesis includes the following Invitrogen components to help assure that your construct meets all of your research, quality, and documentation requirements.

GeneArt Gene Synthesis production times

It is important to note that genes are made-to-order products and can show variations in producibility, depending on the complexity of the DNA sequence. Typical production times are met for > 90% of genes produced. Production time is now faster by 2 days for genes up to 7 kb and by 9 days for genes between 7 – 9 kb. Production time is the number of business days required to synthesize genes in our manufacturing facility. Delivery time is in addition to production time and depends on the destination of the shipment.

*Valid for standard gene synthesis (non-complex, GC content 10–80%). For complex sequences, production times will be longer and these will be communicated prior to production.. **Subject to sequence assessment. Order must be placed by 3:00 p.m. CET. ***We will respond to your email in a 24hr time frame. Please use our Sequence Submission Form to transfer your sequence.

Please note: Rarely, requested sequences are found to be toxic and/or genetically unstable. These production times are only valid for nontoxic sequences that are genetically stable in E. coli.

Rapid synthesis and assembly: Standard turnaround times start at 10 business days (accelerated options available).

Economical: The cost of the GeneArt Gene Synthesis service rivals costs that would be incurred to purchase the oligonucleotides and molecular biology reagents required for gene synthesis, and outsourcing your gene synthesis and cloning means you can focus on your research.

Excellent accuracy: All custom synthesized genes are sequenced as part of our ISO 9001:2015–certified quality management system; we only ship constructs with exact sequence agreement.

"GeneArt it": Save time and labor by outsourcing your gene synthesis and cloning so you can focus on your research.

Gene synthesis is a fast, simple alternative to traditional cloning

Whether you have limited cloning experience or simply want to save time, the GeneArt Gene Synthesis service helps you move your ideas from the planning stage to the laboratory more quickly. Benefit from our experience in successfully producing over 180,000 constructs for customers as diverse as large pharmaceutical companies, biotechnology start-ups, and basic research institutions. The comparison shown below highlights the time and effort saved compared to traditional cloning.

GeneArt Gene Synthesis process

Submitted DNA sequences are designed by our team of highly skilled scientists to include/exclude restriction enzyme sites and translation elements as requested, and are optionally optimized using our GeneOptimizer software for maximum protein production.

Oligonucleotides synthesized in-house, with maximum sequence accuracy, serve as the building blocks for custom gene synthesis.

Genes are assembled using our GeneAssembler process, which facilitates high-throughput gene assembly on an industrial scale for rapid order fulfilment. Using our GeneObserver online tracking system, you can access detailed information 24 hours a day about your order as it progresses through our synthesis process.

Constructs are cloned into one of our standard cloning vectors, or you can specify your own custom vector by adding a subcloning service (additional fees will apply)

All constructs are sequence-verified; only those that match the requested sequence 100% are shipped. The DNA is delivered as 5 µg lyophilized plasmid DNA in microcentrifuge tube

Quality of every gene construct is controlled as part of our ISO 9001:2015-certified quality management system, which helps ensure reliability and reproducibility. A detailed synthesis report including plasmid map, sequence alignment, and restriction map is provided with each construct.

Comparative expression analysis of wild type vs. an optimized human gene. Western blot analysis using α-His antibody. Expression levels of 2 independent transfections per wild type and optimized construct are displayed, showing a 3-fold difference in expression in this particular analysis. Standardization is based on endogenous protein.

Save time with the new express cloning service

Using an alternative production procedure with pre-prepared expression vectors, we can clone your GeneArt synthesized genes directly into selected expression plasmids, saving 4–5 business days compared to the standard workflow with cloning into pMx series vector first.

Turnaround time for expression-ready genes starts at 11 business days (depending on the length of your gene). Adding the gene synthesis express upgrade cuts an additional 2 days, so it is possible to get a turnaround time of 9 business days for your cloned, expression-ready gene.