Chile seed crops require careful agronomic management to assure genetic uniformity and field performance. Seed crop producers must ensure that crops are grown in isolation to avoid cross-
pollination with other cultivars, off-type plants are rogued throughout the growing season, and plant health is monitored carefully. In addition, special harvesting practices are necessary to
assure seed quality. This publication outlines recommendations for seed crop production and harvesting and seed disinfecting, drying and storage. Following these guidelines will help seed growers produce a certifiable crop.

Table 1. NMCIA minimum standards for certified chile seed.

Characteristic

Standard

Pure Seed

<98%

Germination

<80%

Other Chile Types

<1%

Other Seed

<1%

Inert Matter

<2%

Weed Seed

None

The New Mexico Crop Improvement Association (NMCIA) manages seed certification programs in the state. NMCIA-certified seed is guaranteed to meet minimum standards for cleanliness and germination rate. A certified seed-lot contains more than 98% pure seed, has a germination rate greater than 80%, contains less than 1% seed of other chile types or other seeds, contains less than 2% inert matter and contains no weed seeds (Table 1). Certified seed analysis must be performed by a seed lab accredited by the American association of Seed Analysts. The New Mexico Department of Agriculture State Seed Laboratory is an accredited lab.

Certified Chile Seed Crop Production Checklist

Rotate crops. Seed-crop fields must not have been planted with chile the previous year
and, ideally, fields should not have been planted with chile for five or more years. Long-
term chile rotations significantly improve chile yield and quality and reduce diseases
and weeds.

Test soil for nematodes. Test for nematodes in the fall prior to planting. Avoid infested
soils or treat with a nematicide if root-knot nematodes are present. Nematodes usually are
not a problem on sandy soils and are less of a problem on clay soils. Contact your county
Extension agent for information about nematode sample testing laboratories. Test soil for fertility and salinity. Conduct soil tests at least six weeks before planting to allow time to apply any necessary soil amendments.

Balance fertility. Apply soil amendments to balance phosphorus, potassium, calcium and
micronutrients. These soil nutrients should be in the high range, especially when large amounts of nitrogen (>150 lbs/acre) are applied. High phosphorus availability is important for seed yield and quality. Fertility imbalance, especially deficient potassium, reduces seed quality and yield (Harrington, 1960).

Do not over- or under-apply nitrogen. In a study of nitrogen application on ‘Anaheim’ chile, fertilization at 185-215 lbs/acre (210-240 kg/ha) maximized seed yield, with lower
yields above or below these rates (Payero, Bhangoo and Steiner, 1990).

–The best response for seed yield was achieved at 60 lbs/acre (70 kg/ha ) split into three applications: at planting, early fruit set and full fruit size.
–An alternative is to apply nitrogen at 60 lbs/acre (70 kg/ha) at planting and another 30 lbs/
acre (35 kg/ha) when leaf petiole nitrate levels fall to 2,000 parts per million (ppm).

Sample leaves every six to eight weeks after emergence to check fertility. Payero et al (1990) found that the standard recommendation for chile pod production of maintaining more than 5,000 ppm nitrate in leaf petioles reduced seed yield and quality. This finding suggests that chile seed crop nitrogen fertility should be different than fertility for chile fruit crops. Contact your soil and plant testing lab or county Extension agent for chile tissue sampling methods and labs.

Isolate chile seed fields from other chile fields. Chile seed crops must be isolated from
other chile fields by at least three miles for the seed to be “certified,” one-half mile for seed
to be “registered”and one mile for seed to be designated “foundation seed.” Ideally, the
seed crop should be isolated from other chile crops by one mile.

Grow seed crops for seed only. Use red fruit only as a by-product of seed extraction.
The seed crop cannot be harvested partially for green chile.

Harvest only healthy plants for seed. Diseased fields, or diseased sections within a field,
should not be harvested for seed. A field, or portion of a field, cannot be harvested for
certified seed if more than 5% of the plants show symptoms of chile wilt (Phytophthora,
Verticillium or Fusarium) or curly top virus. Chile seed fields also must be free from
bacterial leaf spot disease. Certified seed must be treated with sodium hypochlorite (bleach solution) or have a negative lab test for bacterial leaf spot. For more information about chile diseases, please refer to Chile Pepper Diseases, NMSU Cooperative Extension Service Circular 549.

Allow seed crops to properly mature. Late in the season, fertilizer applications should be
stopped and irrigation should be reduced. This will stimulate fruit to ripen and partially
dry the fruit to a leather like condition. Chile crops that are growing actively during a
killing frost produce poor quality seed, because fruits decompose rapidly and there is a
greater proportion of immature fruit.

Don’t use ripening hormone on seed crops. Prior to harvest, chile seed crops should not
be treated with the ripening chemical ethylene (Ethephon or Etheryl) or with crop desiccants (leaf droppers) like sodium chlorate.

Harvest only red, ripe chile. Immature, green and diseased fruits should be removed completely. Seeds from immature peppers lack vigor and germinate poorly. They have a fish-mouth appearance because of incompletely developed endosperm (Bosland and Votava, 1999).

Extract seed immediately after harvest. Crops should be processed as soon as possible
after harvest, preferably within 24-72 hours. Warmer weather or storage conditions cause
rapid crop deterioration after harvest.

Seed Disinfection Treatments

Certified seed must have a negative lab test for bacterial leaf spot disease or be treated with sodium hypochlorite. Seed producers should take the following steps after seed crops are harvested:

Extracted seed should be soaked in a 3% sodium hypochlorite solution at 50°-75°F (10°-25°
C) for no longer than 20 minutes to disinfect seed from diseases, such as bacterial leaf spot (Khah and Passam, 1992).

Keep the soaking solution clean and free from dirt and organic matter.

Household bleach usually contains about 5% sodium hypochlorite. Do not use bleach
products that contain ingredients other than sodium hypochlorite, such as sodium
hydroxide or fragrances.

To make a 3% sodium hypochlorite solution from household bleach, mix three parts
plain household bleach with two parts water. To avoid seed damage, do not soak seed
longer than 20 minutes. An alternate recommendation is to soak seeds in a 1% sodium
hypochlorite solution for 40 minutes (Goldburg, 1995). Mix 20% household bleach with
80% water (one part bleach to four parts water) to make a 1% solution.

Sodium hypochlorite seed treatments can disinfect seed and stimulate germination when
properly applied (Fieldhouse and Sasser, 1975), but soaking seed too long or in concentrations higher than 3% can injure seeds and reduce germination.

Sodium hypochlorite’s effect on chile seed germination is influenced by the soaking time
and temperature, and seed age. Recently harvested seed is more reactive to the treatment.
Longer soaking times at low sodium hypochlorite concentrations may not thoroughly
disinfect seed, especially when the soaking solution is dirty (Khah and Passam, 1992).

Check chlorine levels in soaking tanks several times a day and add sodium hypochlorite
to maintain proper chlorine concentration. Time soaking carefully. Order chlorine solution testing equipment from swimming pool supply companies or search for suppliers on the Internet.

Seed Drying and Handling

After disinfecting and cleaning seed, it should be dried with warm (85°F) air to 10% moisture and graded to remove lightweight seed.

Recently harvested seed of some pepper cultivars or seed-lots can exhibit dormancy or delayed germination. After-ripening or curing seed at 70°-80°F for six weeks can remove dormancy (Randle and Homna, 1981).After processing and curing, seed should be stored in a cool (40-50°F), dry, dark place in airtight, waterproof containers and protected from insects and rodents.

Seed can be treated with a registered seed fungicide just prior to planting to avoid seedling
diseases. In cool, wet soils, seed fungicides may not control some seedling diseases, such
as damping-off. A list of registered seed fungicides is available from agricultural supply companies or from the plant pathologist at NMSU’s Extension Plant Sciences Department at (505) 646-2875.

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