Syk antibody [SPM147]

Cat No. GTX15238

Application Note

For IHC-P: Use at a dilution of 1-100 for 10 min. at RT. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For IP: Use at a concentration of 2ug antibody/mg protein lysate. For WB: Use at a concentration of 1 μg/ml. Predicted molecular weight: 72 kDa. Optimal dilutions/concentrations should be determined by the researcher

Calculated MW

Positive Control

Form

Buffer

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide - amino acids 313-339 of Human Syk.

Purification

Protein G affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Spleen Associated Tyrosine Kinase,P72-Syk,Syk

Cellular Localization

Cytoplasmic (probable)

Background

SYK is a positive effector of BCR stimulated responses. It couples the B cell antigen receptor (BCR) to the mobilization of calcium ions either through a phosphoinositide 3 kinase dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C gamma dependent pathway, when phosphorylated on Tyr 342 and Tyr 346. Thus the differential phosphorylation of SYK can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ions.