The Effects of 1,25 Dihydroxyvitamin D3 and Four Chemical Analogs on Components of the Cell Cycle Regulatory System in Two Cancer Cell Lines

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Abstract

Cyclins, cyclin dependent kinases (CDK's), and cyclin dependent kinase
inhibitors (CDI's) have recently been found to play integral roles in
controlling cell proliferation. The compound 1,25 dihydroxyvitamin D3, a
hormone metabolite of vitamin D, has been shown to act through its cognate
nuclear receptor to induce terminal differentiation sequences in cancer cell
lines, thereby effectively stopping their growth. It has been shown this effect
is achieved by the hormone's regulation of some of the above-mentioned
components of the cell regulatory system. This project characterizes the effect
of vitamin D3 on more of these cyclins, CDK's, and CDI's in a U937
myelomonocitic cell line and a MCF-7 immortal breast cancer cell line.
Additionally, this project examines the effects of four chemical analogs of 1,25
dihydroxyvitamin D3, and shows one of them, JK1624-3, to be almost 100
times more potent in treating cancer cells than its parent compound.