The QIAamp DNA Mini QIAcube Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Performance

The QIAamp DNA Mini QIAcube Kit yields DNA sized up to 50 kb: DNA of this length denatures completely and has the highest amplification efficiency. The purified DNA may be used in a number of applications, including:

Viral research

Bacterial research

Fungal research

Cancer research

Human genetic testing research

Paternity testing

Forensic analysis

The QIAamp DNA Mini QIAcube Kit simplifies isolation of DNA from human tissue samples with fast spin-column procedures. The kit includes rotor adapters that are preloaded with QIAamp spin columns and elution tubes, delivering greater convenience and time savings (see figure "Significant time savings"). Furthermore, ease of use is increased and user errors minimized. Waste is reduced, because the content of the dedicated kit is tailored to purification on the QIAcube and the superfluous tubes that are required for the manual procedure are not included.

Principle

DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. No phenol-chloroform extraction is required. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure DNA to be eluted in either water or a buffer provided with the kit. QIAamp DNA technology yields genomic, mitochondrial, bacterial, parasite, or viral DNA from human tissue samples ready to use in PCR and blotting procedures.

Procedure

The dedicated QIAamp DNA Mini QIAcube Kit is designed with preloaded spin columns and elution tubes in rotor adapters for the QIAcube, eliminating the risk of errors due to incorrect loading of rotor adapters. The dedicated kit is tailored to QIAcube requirements, reducing waste.

A simple protocol (lyse, bind, wash, and elute) and an automated protocol on the QIAcube requires minimal user interaction. The award-winning QIAcube uses advanced technology to process QIAGEN spin columns, enabling seamless integration of automated, low-throughput sample prep into laboratory workflows. All steps in the purification procedure are fully automated — up to 12 samples can be processed per run. The QIAcube together with the dedicated QIAamp DNA Mini QIAcube Kit provides a winning combination for fast, easy, and convenient DNA purification.

Applications

The QIAamp DNA Mini QIAcube Kit is for automated isolation of genomic, mitochondrial, parasite, or viral DNA on the QIAcube. Sample sources include: