Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1(67), CK2(65.5), CK3(64), CK4(59), CK5(58), CK6(56), CK7(54), CK8(52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16 (48), CK17 (46), CK18 (45), CK19 (48) and CK20 (46).

Store undiluted at 2-8°C. DO NOT FREEZE!This products is photosensitive and should be protected from light. Shelf life: one year from despatch.

Format

Label:

PE

Purification:

Protein A/G Chromatography

Buffer System:

10mM PBS

Preservatives:

0.05% Sodium Azide

Stabilizers:

0.05% BSA

State:

Liquid purified IgG fraction from Bioreactor Concentrate

Species Reactivity

Species reactivity (tested):

Human, Monkey, Cow, Dog, Rabbit, Mouse, Rat, Chicken.

Specificity

Specificity:

Twenty Human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI < 5.7) and basic (pI > 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5, CK6, CK8, CK10, CK14, CK15, CK16, and CK19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. This Monoclonal Antibody is a broad spectrum anti pan-Cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains Cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.Cellular Localization: Cytoplasmic.