Trouble began on the sixth day of the flight, November 17. The capsule developed an atmospheric leak, the pressure first dropping from 760 to 380 mm of Mercury. With the drop in cabin pressure all the animal test subjects died. It would have killed any Cosmonaut not wearing a spacesuit.

The first attempt for a Zond 4 follow-up launched on April 22. It failed when the Launch Escape System sent an erroneous abort command at T+260 seconds and shut down the Proton booster’s second stage. The escape rocket fired and pulled the descent module to safety.