Bovine Mercaptalbumin and Non-mercaptalbumin Monomers

Abstract

The monomer macroheterogeneity of commercial bovine serum albumin preparations is entirely due to oxidation of the mercaptalbumin sulfhydryl group. When the sulfhydryl group is oxidized to oxidation states higher than disulfide, circular dichroism and fluorescence data show that a tryptophanyl and a cystinyl residue are perturbed. Measurements conducted… (More)