Background: Diabetes has the most important role in development of tissue damage, and by affecting intercellular matrices, may lead to structural and functional changes that ultimately cause failure of related tissue or organ. Exercise and herbal medicine can be effective in reducing organ failure. This study aims to assess the effect of aerobic training combined with consumption of portulaca oleracea supplements on Metalloproteinase Matrix 2 (MMP2), Metalloproteinase Matrix 9 (MMP9), and Tissue Inhibitor of Metalloproteinase Matrix (TIMP1) in diabetic women type II.Materials and Methods: 28 women with type II diabetes and average age of 51 years were divided into 4 groups of control (CG), exercise (EG), exercise-supplement (E-SG), and supplement (SG). A course of exercise was designed to cover 60-minute, 3 sessions per week for 8 weeks, with 50-70% maximum heart rate intensity. A 7.5 grams daily supplement of portulaca oleracea was administered, consisting of 2.5 grams with luncheon and 5 grams at dinner. Blood samples were taken before and after 8 weeks of intake of supplement and exercise following 48 hours of non-consumption of supplement and 12 hours of fasting. Data were analyzed using variance analysis model.Results: After 8 weeks, MMP2, MMP9 levels significantly reduced in all groups (P<0.05). But the difference between groups was insignificant. TIMP1 level significantly increased in all groups but control, and there was a significant difference in TIMP1 level between control and supplement groups (P<0.05).Conclusion: According to the results, aerobic training together with purslane seed intake, did not positively affect matrix metalloproteinase, but its inhibitors were effective. Thus, further study is required for more accurate results.