Aliens vs. Predator: Requiem was released on December 25, 2007 and received a largely negative response from film critics. The film grossed $9.5 million on its opening day and took in a worldwide gross of $128.9 million in theaters. According to Home Media Magazine, the film debuted at #1 in sales and rentals on Blu-ray and #2 on DVD when it was released on home video on April 15, 2008. Since then, the film has gained $28,550,434 in home video sales, bringing its total film gross to $157,461,400.

The film begins immediately following the events of Alien vs. Predator, aboard a Predator spaceship which is leaving Earth carrying dead Alien, living facehuggers, and the body of the Predator that defeated the Alien queen. A chestburster erupts from the dead Predator's body; it is a new creature that is a hybrid of Alien and Predator characteristics (referred to by the production team as the "Predalien"). It quickly matures into an adult and begins killing Predators throughout the ship. A Predator's weapons fire punctures the hull and the ship crashes in the forest outside of Gunnison, Colorado.

With the Predators dead, the Predalien and several facehuggers escape, implanting embryos into a nearby father and son and into several homeless people living in the sewers. A distress signal from the wrecked ship reaches the Predator home world and a lone Predator responds, traveling to Earth and using its advanced technology to observe the cause of the crash and to track the facehuggers. It begins to erase the evidence of the Aliens' presence by destroying the crashed ship and using a blue liquid to dissolve the bodies of the facehuggers and their victims.