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Alexandrov Cell-Free Protein Expression Kit (PEK)
(Kit #
1000000065
)

This collection contains a set of 91 plasmids: 2 empty backbone control plasmids (pLTE EGFP and pCellFree_G03) and 89 open reading frames cloned into the pCellFree vector, which enables protein expression in any in vitro translation system.

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

Description

The Alexandrov Cell-Free Protein Expression kit represents a set of 89 open reading frames cloned into the pCellFree vector, which enables protein expression in any in vitro translation system. The gene set was carefully chosen to perform statistically relevant benchmarking of cell-free expression systems. The set of proteins encoded is one of the most popular ones in the biomedical research community and range in size form 4 to 156 kDa, which enables the user to characterize the efficiency of their cell-free system in correlation to the size of the product. The analysis can be conveniently performed using the N-terminal eGFP tag carried by all constructs.

Please note that Well-G6 is a duplicate of Well-G5. Both Wells contain plasmid pCellFree_G03 STAT3.

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.