NOTE: Survival surgery should be performed using aseptic procedures with sterile surgical gloves, masks, and instruments. The surgical
area should be uncluttered and disinfected. Refer to Hoogstraten‐Miller and Brown () for a detailed description of setting up the surgical area. Injection material should be ready and kept on ice before the
surgical procedure.

Alternate Protocol 2: Vector Delivery by Intrasplenic Injection

NOTE: All solutions used for vector dilution should be sterile and tissue culture grade. Only sterile disposable tubes and sterile
filtered pipet tips should be used. To avoid contamination, vector samples should be prepared in a culture hood dedicated
for rAAVs.