Figure 6.

Titration of progeny viruses. Titer of progeny viruses produced from the cell was measured by TCID50 assay in 384-well
plate format with 4 wells per dilution of virus. Ten (10) μL of 10-fold serial dilutions
of progeny virus containing medium from respective samples (drug treated or untreated)
were used to infect fresh Hep-2 cells in a 384-well format. After 6 days incubation
the inhibitory effects of the compound and drug were assessed using Cell Titer Glo.
A well showing a luminescence signal less than the mean of the non-infected control
signal minus five times the standard deviation of the control was regarded as positive
for infection.