Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

WB

Use a concentration of 0.5 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).Can be blocked with Human Olig2 peptide (ab111666). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ICC/IF

Use at an assay dependent concentration. PubMed: 20025521

IHC-Fr

1/200. (see Abreview and notes)

IHC (PFA fixed)

1/1000.

Target

FunctionRequired for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.

Tissue specificityExpressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.

Involvement in diseaseNote=A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.

Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.

DomainThe bHLH is essential for interaction with NKX2-2.

Cellular localizationNucleus. Cytoplasm. The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2.

If you could specify which image you are referring to, I can try to help you further. However, regarding the references, I am sorry to let you know that we do not have unrestricted access to all journals...

Thank you for your patience in this matter.
I have received a reply from the originator of ab42453 (Olig2 antibody). The exact immunogenic peptide sequence is proprietary information. However, we can state that it is within the following 50 amino...