This assay has high sensitivity and excellent specificity for detection of Rat LDL. No significant cross-reactivity or interference between Rat LDL and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat LDL and all the analogues, therefore, cross reaction may still exist.

Samples

Serum, Plasma, Biological Fluids

Assay Type

Sandwich

Detection Range

0.156-10?g/mL

Sensitivity

Min: 0.094?g/mL; Max: 10?g/mL

Preparation and Storage

Store at 4 degree C.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Product Note

Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Other Notes

Small volumes of LDL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LDL purchase

MBS2508037 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the LDL, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10?g/mL in biological research samples containing LDL, with an estimated sensitivity of Min: 0.094?g/mL; Max: 10?g/mL. The ELISA analytical biochemical technique of the MBS2508037 kit is based on LDL antibody-LDL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LDL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LDL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LDL in Rat serum, plasma and other biological fluidsPrinciple of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat LDL. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat LDL and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LDL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat LDL. You can calculate the concentration of Rat LDL in the samples by comparing the OD of the samples to the standard curve.

L-lactate dehydrogenase

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Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.