This year, the Phi Zeta Research Emphasis Day will be held on Wednesday, September
28. The organizing committee would like to encourage all faculty, post-doctoral researchers,
advanced studies students, and professional students to submit abstracts for this
event. All presentations will be in poster format. Although summer scholars do not
need to reformat or reprint their posters for Phi Zeta, they do need to submit an
abstract.

Eligibility to Compete

Any SVM Graduate, Professional Students or Postdoctoral researchers or non-SVM students
mentored by an SVM faculty member.

Those posters presented by advanced studies students, professional students
and postdoctoral researchers will be judged by a panel of non-SVM scientists for monetary
prizes in four categories: (1) Dissertation (PhD) students 2) Basic research for
thesis (M.S.), professional students, and house officers; (3) Clinical research for
thesis (M.S.), professional students, and house officers; and 4) Post-doctoral researchers.
A total of 4 prizes will be awarded to groups 1-3 (1st ($300), 2nd ($200), 3rd ($100)
and 4th ($50)) whereas 3 prizes (1st, 2nd and 3rd) will be awarded to group 4. The
clinical research category is relatively new and is defined as research that:

focuses on a question originating from clinical veterinary medicine

is relevant to the animal species associated with the clinical question

may use all available research methods (from the animal to the molecule)

The committee also encourages the participation of SVM faculty members. Faculty wishing
to display their posters only need to submit the names of the authors, abstract title(s)
and the number of poster boards required.

Deadline

The deadline for abstract submissions is 5:00 p.m., Tuesday, September 12. Please note that any abstracts submitted after September 12 will not be accepted.
Please submit your abstracts to Ms. Jamie O’Quinn via email jamieo@lsu.edu. Submitted abstracts will be used to prepare a Phi Zeta Research Emphasis Day Program.
Abstracts will be distributed to the non-SVM judges in advance of the poster presentation.
Please note that SVM Media Services (Mr. Michael Broussard) is no longer printing
posters. Options for printing are at Middleton Library, or by some other personal
arrangement.

This event is an exceptional opportunity for individuals in the SVM scientific community
to present the results of their research efforts to other members of the SVM and LSU
communities. It serves as an important showcase for SVM Research activity. We anticipate
the presence of the Provost and Vice Provost for Research and Economic Development
from the main campus.

Guidelines for Abstract Preparation

Advanced studies, professional, and undergraduate students as well as Post-doctoral
researchers should submit abstracts and a note stating in which of the 4 categories
they are competing. Faculty wishing to present posters should submit the author names,
abstract title(s), and the number of poster boards required.

The abstracts should be submitted as a single-spaced, typed pdf document. The font
should be at least 10 characters per inch (font size 12) and the page should have
a minimum of one-inch margins on all sides. The abstract body is limited to 250 words
(Note that the 250 word limit does NOT include the title, authors and departmental
information). The abstract should contain the following sections. Template abstracts
are also enclosed for additional information.

Title: Adequately describe the study (to be consistent, please capitalize the first
letter of each word in your title. For example, “Guidelines for Abstract and Poster
Preparation”).

Authors: Include all authors. (To be consistent, please list the authors’ names using
the initials for the first name followed by the last name and list the participant’s
name in bold, e.g, J. Smith).

Rationale: State the rationale for the study and provide appropriate background information
and hypothesis or objectives. Indicate the objective and purpose of the research,
the hypothesis that was tested or a description of the problem being evaluated or
analyzed.

Results: Clearly presented and consistent with the experimental methods and design.
Present as clearly and in as much detail as possible the observations/outcome of the
study. Please summarize specific results.

Conclusions: Accurate interpretation of results within the context of the original
hypotheses/objective(s). Explain the significance of the findings/outcome of the
study for prevention, treatment, care and/or support, and future implications of the
findings.

Significance/Impact/Implications: Place the results and conclusions of the investigation
within the context of our current knowledge of the subject area.

Template Abstract 1 - Basic Science

Background and Rationale: New advances in stem cell technology, including induced
pluripotent stem cells (iPSC), offers new hope for patients with neurological disease
and spinal cord injuries. Therefore, we evaluated the ability of canine iPSC to be
differentiated into neural progenitor cells (NPC) in vitro as a precursor to clinical
trials in dogs with spinal cord injury.

Approach: iPSC were generated from canine fibroblasts and characterized based on phenotype,
gene expression analysis, lineage differentiation, and teratoma formation. Canine
iPSC were then induced to differentiate into NPC by culture in defined medium supplemented
with specific growth factors. NPC were characterized by phenotype, flow cytometry,
immunofluorescence, and gene expression analysis.

Results: Canine iPSC could be readily induced to differentiate into NPC following
2-3 weeks in culture. Specific culture conditions led to enrichment of NPC for cells
with characteristics of oligodendrocytes, astrocytes and neurons. NPC did not form
teratomas in mice, whereas the parental iPSC cells did.

Conclusions/Implications: Canine iPSC can be induced to form NPC in vitro by altering
cell culture conditions, cell substrate, and addition of specific growth factors.
These studies provide evidence that iPSC technology can be used to generate NPC for
use in neural regeneration in dogs with neurological injuries.

Template Abstract 2 - Basic Science

Key Role for Scavenger Receptor B-I In the Integrative Physiology of Host Defense
During Bacterial Pneumonia

K.M. XXX, M.B. XXX, Department of XXXXXXXXXXXXXXX

Rationale: Scavenger Receptor B-I (SR-BI) is a multi-recognition receptor mostly studied
in the arena of atherosclerosis due to its role in cellular uptake of cholesterol
ester from high density lipoprotein (HDL). Recently, SR-BI has also been reported
to play a role in clearance of lipopolysaccharide (LPS) from the plasma.

Results: Compared to SR-BI+/+ counterparts, SR-BI-/- mice suffered markedly increased
mortality during pneumonia, in conjunction with higher bacterial burden in lung and
blood, deficient induction in the plasma of the stress glucocorticoid corticosterone,
higher serum cytokines, and increased peripheral organ injury. SR-BI-/- mice had significantly
enhanced PMN recruitment to the airspace as well as increased BALF TNF-α, G-CSF, and
CXCL5 after pulmonary exposure to either Kp or LPS. This was associated with defective
clearance of LPS from the SR-BI-/- airway and increased cytokine production by SR-BI-/-
macrophages. SR-BI-/- PMNs displayed decreased phagocytosis and a dramatic defect
in intracellular bacterial killing.

Template Abstract 1 - Translational/Clinical Science

Pharmacokinetics and Pharmacodynamics of an Extended Release Buprenorphine Formulation
in Dogs

XXXX

Department of XXX, College of Veterinary Medicine, XXX University,

Rationale: The options for effective and safe long-term post-operative analgesia in
canine patients are very limited. The purpose of this study was to describe the pharmacokinetics
and pharmacodynamics of an extended-release buprenorphine (ERB) formulation in healthy
adult dogs. We hypothesized that plasma concentrations associated with therapeutic
efficacy would be maintained for 72 hours after a single subcutaneous administration
of ERB.

Results: Maximum plasma concentrations (median, range) of buprenorphine in dogs receiving
ERB was 5 (4.3-11.0) ng/mL, which occurred at 8 (4-36) hours (Tmax). Therapeutic plasma
concentrations (>1 ng/mL) occurred from 0.5 to 72 hours after administration in most
(5/6) dogs. Thermal withdrawal latency was significantly prolonged for 48 hours in
dogs after ERB administration and for up to 4 hours when dogs received IVB. Sedation
scores were not significantly different in dogs receiving IVB or ERB, and no serious
adverse effects were seen in either treatment group.

Conclusions/significance: These results suggest that a single dose of 0.2 mg/kg ERB
is safe, provides consistent therapeutic plasma concentrations, and exhibits prolonged
analgesia in healthy dogs.

Template Abstract 2 - Translational/Clinical Science

Rationale: Low-tidal volume ventilation (LTVV) has been shown to significantly reduce
mortality in patients with acute respiratory distress syndrome (ARDS). We investigated
the rate of clinician recognition of ARDS in a single center and sought to identify
clinical factors associated with under-recognition of ARDS.

Methods: From a prospective cohort of 363 patients admitted from the emergency department
to the intensive care unit (ICU), we identified 72 patients who met Berlin criteria
for ARDS as determined by review of chest radiographs and clinical information from
the first 5 days in the ICU. We then tested the association of clinician recognition
of ARDS with patient-level clinical variables and used backward stepwise multivariable
logistic regression to identify independent predictors of ARDS recognition.

Results: Within 7 days of meeting diagnostic criteria for ARDS, 40% of patients had
clinical documentation of the diagnosis of ARDS; 51% had ARDS mentioned as part of
a differential diagnosis; and 40% had discussion of LTVV strategies in the medical
record. Overall, 53% of patients with ARDS had either recognition of ARDS or discussion
of LTVV in their medical record. Clinician diagnosis of ARDS was associated with implementation
of LTVV (<8 mL/kg IBW) within 2 days of meeting diagnostic criteria (mean TV 7.3 vs.
8.5 mL/kg IBW, p=0.03).

Conclusions: Clinician recognition of ARDS remains poor, with many cases going unrecognized.
Severity of ARDS as measured by Lung Injury Score was the strongest predictor for
clinician recognition of ARDS. Clinician recognition of ARDS was associated with use
of LTVV strategies.

Guidelines for Poster Preparation

The maximum size of each poster is 3 feet (36 inches) high by 4 feet (48 inches) wide.

The poster boards are covered with felt. To hang the posters on the board, please
use Velcro behind the poster board.

Poster Judging Process

Judges are chosen from members of both the scientific and veterinary practitioner
(non-SVM) community from across the entire state as well as the entire LSU system.

Although judges participating in the research emphasis day will be identified as a
group, the identity of judges responsible for reviewing specific posters will NOT
be released.

When possible, judges are assigned to posters using a computer random number generator.
Judge assignments are then manually reviewed to make sure that no judge reviews their
own (or their students’) posters.

Judge assignments are also reviewed to make sure that the same judge does not review
the same student multiple times, and that “pairs” of judges are not assigned to review
multiple posters together.

Judges will provide a numeric score of each poster. These scores will then be tallied
electronically. The posters in each category will then be ranked and this ranking
will be used to determine first, second, etc. place posters.

Specific written commentary is encouraged and may be provided by the reviewers. This
commentary will be transcribed and will be made available to the poster presenters,
although it will not itself factor into the ranking process.

If a presenter wishes to see their raw score after the competition, they will be allowed
to do so although the score alone may not convey much information. The presenters
will NOT be allowed to view the scores of others.