Standard Primers

One click ordering for universal primers

In order to enable fast and convenient ordering of sequencing primers that are widely used to sequence inserts in standard cloning vectors, we have assembled a list of primer sequences that you can order with a single click. Simply select the primer and add it to the cart. We have also tailored the post-synthesis work flow to ensure that these primers have the quality that will provide the best sequencing results.

Typically, the quality of the primer is not critical for the amplification of sequences of interest when the amplified PCR product is analyzed by agarose gel electrophoresis. On the contrary, when the primers are used for sequencing a template, the quality of the primers is critical for the sucess.

Mutations are commonly identified by direct sequencing of the PCR product. The primer quality is therefore even more critical when you want to detect or verify single base variants:

Genotyping by sequencing

Microsatellite analysis

Verification of Site-Directed Mutagenesis experiments

The standard sequencing primers ordered via the Standard Sequencing primer have the following predefined parameters:

Features of Standard Primers

Details

Synthesis Scale

50 nmol

Modifications

none; only pre-defined sequences

Delivery Format

Dry; in tubes

Available Lengths

15–30 (predefined)

Default Purification

HPSF (High-Purity Salt-Free)

Production days

2 days*

*please add the shipping days to the production days to determined the delivery days.

Over the years, our scientists have sequenced several templates using the primer sequences below. These primers are added to your samples for no extra charge when indicated in your sequencing orders. We also encourage you to order these primers and prepare your 'premixed' samples. Amongst the different types of samples sequenced (excluding samples in ready2load plates), the premixed samples that require standard sequencing chemistry are processed at the most rapid pace and the sequence data is released earliest. To order any of the primers listed here, please use the Standard Primers link to avail a one-click ordering protocol for a HPSF-purified sequencing grade primer. The delivered primer can be used in over >500 sequencing reactions.