The goal of my project is to stream a recorded file to the psoc then to filter it with the psoc and to get back the filtered file to the computer then to analyse it.

Do you know a way to pass a audio file using usb isochronous transfer and ( I tried example usbfs) and I cant figure out how much packets can i pass between the pc and psoc5 total?

I saw that i can pass txt files (64bytes)and it works well but not in the Isochronous transfer( which i think i need) it passes only part of the information of the file(half or less) what can I do in order to pass 10-20MB .wav file?

Try this project. It has a lot of excess - part can be removed. To test you can probably use the generators, creation for the sound cards. A lot of them on the Internet. I use my, he is on this page. But note that in most cases the signal my be be distorted integrated filter PC. Pay attention to the draft Project # 102 - USB Audio using the PSoC 5L