Part:BBa_K2560089

This is the Phytobrick version of terminator BBa_1002 and was build as a part of the Marburg Collection. Instructions of how to use the Marburg Collection are provided at the bottom of the page.

Overview

Terminators are sequences downstream CDSs promoting dissociation of the transcriptional complex. In prokaryotes two kind of transcription terminators are reported. The first ones are Rho-independent terminators. This sequences build up hairpin structures during transcription disrupting the transcriptional complex. The mechanism of disruption is hypothesized to occur through allosteric effects of the hairpin binding and competitive kinetics
( Farnham PJ and Platt T.,1981)
The other group are Rho-dependent terminators which require Rho and ATP. This terminators are found in bacteria and phages. The Rho factor binds to a cytosine-rich sequence on the mRNA where it hydrolyzes ATP, translocates down the mRNA and stimulates the dissociation of the transcription complex when contact is established
( Banerjee et al.2006).

Sequence and Features

Assembly Compatibility:

10

COMPATIBLE WITH RFC[10]

12

COMPATIBLE WITH RFC[12]

21

COMPATIBLE WITH RFC[21]

23

COMPATIBLE WITH RFC[23]

25

COMPATIBLE WITH RFC[25]

1000

COMPATIBLE WITH RFC[1000]

Marburg Toolbox

We proudly present the Marburg Collection, a novel golden-gate-based toolbox containing various parts that are compatible with the PhytoBrick system and MoClo. Compared to other bacterial toolboxes, the Marburg Collection shines with superior flexibility. We overcame the rigid paradigm of plasmid construction - thinking in fixed backbone and insert categories - by achieving complete de novo assembly of plasmids.

Figure 3: Hierarchical cloning is facilitated by subsequent Golden Gate reactions. Basic building blocks like promoters or terminators are stored in level 0 plasmids. Parts from each category of our collection can be chosen to built level 1 plasmids harboring a single transcription unit. Up to five transcription units can be assembled into a level 2 plasmid.