Positive Controls

Histology Controls

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References

Reviews

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Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells.

Higher vertebrates express six actin isoforms encoded for by distinct genes. Actin isoforms are highly evolutionarily conserved. Two striated muscle isoforms, alpha skeletal and alpha cardiac, two smooth muscle isoforms, alpha smooth and gamma smooth together with two cytoplasmic isoforms, beta cytoplasmic and gamma cytoplasmic are encoded by genes on chromosomes 1, 15, 10, 2, 7 and 17 respectively. All actin isoforms have highly homologous nucleotide sequences with the heterogeneity most notably in the N terminal region. However, small differences in primary structure here and elsewhere affect the conformational state of the various isoforms and their affinity for a range of actin binding proteins (ABPs). The range of affinities for ABPs suggests that where multiple globular monomeric (G-actin) forms exist at the same site, polymerisation may result in the formation of filamentous (F-actin) enriched for one particular isoform. Defects in actin beta are a cause of juvenile dystonia, a condition that results in movement disorders due to disordered muscle tonicity, abnormal posture, development malformations and sensory hearing loss.

Intended Use

Target Species

Human

Species Cross-Reactivity

Target Species

Cross Reactivity

Mouse

Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Reconstitution

Preparation

Antiserum Preparation

Antiserum to human Actin beta(N-Terminal) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Preservative Stabilisers

0.02% Sodium Azide (NaN3)

Immunogen

A peptide corresponding to an 16 amino acid sequence from near the amino terminus of human Actin beta.

Purity

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Reagents In The Kit

Preparing The Antibody

Test Principle

Buffer Solution

Phosphate buffered saline

Storage

Store at +4oC or at -20oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name

Yes

No

Min Dilution

Max Dilution

Immunohistology - Frozen

1.0ug/ml

Western Blotting

1.0

2.0ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.