The chromatographic
behaviors of sulfachloropyridazine (SCP) and sulfadiazine (SDA) have been
studied by HPLC with molecularly imprinted polymer (MIP) as the stationary
phases. Molecularly imprinted
polymer with SCP as template and an aqueous sodium dihydrogenphosphate buffer solution/acetonitrile (3:2, v/v) as mobile
phase were found to be the best chromatographic system for achieving important
separations of SCPand SDA from their mixtures. In order to compare the chromatographic data from the
stationary phase, separation factors (α) were given. The values of α were 1.86
and 1.67 showed that the MIPs were able to recognize structurally subtle
differences from the template molecule. The binding results from SCP and SDA
solution performed by SCP-MIP showed specific binding towards SCP rather than
SDA on the host-guest system. The values of capacity factor for SCP were
measured and experimental results were further used for simulation to obtain
the binding isotherms. The principal advantage of this method is that SCP-MIP
can recognize SCP and SDA.