Microscopy product ID: 6659

Component

2D Image

Description

Single tilt image of a 250 um thick section of a Drosophila S2 cell infected with flock house virus (FHV-1) imaged with intermediate voltage electron microscopy, taken at zero degree tilt. The dark specks are colloidal gold particles that have been applied to the surfaces of the section to serve as fiducial marks for subsequent alignment.

Segmentation

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Full resolution data file

File Size

3.22 MB

File Format

ZIP

Description

Zip file containing original trace files (FHVmito2.trace and FHVmitonew.trace), along with some of the surface files in Synu (*.synu) and open inventor (*.iv) format. It appears that only some of the surface renderings for the rc objects are available; the rest will have to be regenerated; no surfaced objects associated with mitochondrial membranes were submitted. An animation of the segmented mitochondrion and the viral spherules in Quicktime format is also included (6659_seg.mov). Please note that Jinx (http://ncmir.ucsd.edu/downloads/jinx.shtm) can be used to read traces generated with Xvoxtrace. Jinx requires the original reconstruction file, available from the "Reconstruction' download page.

Reconstruction

Description

Single computed slice through a tomographic reconstruction of mitochondria from Drosophila S2 cells infected with flock house virus (FHV-1). To see the relationship between the 2D tilt image and the reconstructed area, please view the image map. Note that the reconstruction (inset on image map) is flipped vertically relative to the original tilt data.

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Full resolution data file

File Size

1.58 GB

File Format

ZIP

Description

Zip file (FHV2_vol.zip) containing a tomographic reconstruction of mitochondria from Drosophila S2 cells infected with flock house virus (FHV-1). Both the .img and .hdr files are included (fhv2.sub.img/hdr).

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