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Introduction

This product is intended for positive magnetic isolation of CD8+ T cells from lymphoid organs. The supplied protocol describes magnetic labeling and isolation from 5x107 cells. In the first step FlowComp™ Mouse CD8 Antibody (rat IgG2a antibody against mouse CD8) bind to the target cells. In the second step CD8+ T cells that have bound the specific antibodies are captured by the Dynabeads. In the last step beads are removed from the cells.

For flow staining of cells after isolation it is recommended to use Caltag clone 5H10 as primary fluorescent antibody. Avoid using secondary antibodies specific for rat antibodies for flow cytometry staining.