RT Journal Article
SR Electronic
T1 Identification of genes differentially regulated by interferon α, β, or γ using oligonucleotide arrays
JF Proceedings of the National Academy of Sciences
JO Proc Natl Acad Sci USA
FD National Academy of Sciences
SP 15623
OP 15628
DO 10.1073/pnas.95.26.15623
VO 95
IS 26
A1 Der, Sandy D.
A1 Zhou, Aimin
A1 Williams, Bryan R. G.
A1 Silverman, Robert H.
YR 1998
UL http://www.pnas.org/content/95/26/15623.abstract
AB The pleiotropic activities of interferons (IFNs) are mediated primarily through the transcriptional regulation of many downstream effector genes. The mRNA profiles from IFN-α, -β, or -γ treatments of the human fibrosarcoma cell line, HT1080, were determined by using oligonucleotide arrays with probe sets corresponding to more than 6,800 human genes. Among these were transcripts for known IFN-stimulated genes (ISGs), the expression of which were consistent with previous studies in which the particular ISG was characterized as responsive to either Type I (α, β) or Type II (γ) IFNs, or both. Importantly, many novel IFN-stimulated genes were identified that were diverse in their known biological functions. For instance, several novel ISGs were identified that are implicated in apoptosis (including RAP46/Bag-1, phospholipid scramblase, and hypoxia inducible factor-1α). Furthermore, several IFN-repressed genes also were identified. These results demonstrate the usefulness of oligonucleotide arrays in monitoring mammalian gene expression on a broad and unprecedented scale. In particular, these findings provide insights into the basic mechanisms of IFN actions and ultimately may contribute to better therapeutic uses for IFNs. IFN,interferon;2–5A,2′-5′ oligoadenylate;PKR,double-stranded RNA-activated protein kinase;STAT,signal transducer and activator of transcription;IRF,IFN regulatory factor;VEGF,vascular endothelial growth factor;VRP,VEGF-related protein;GAPDH,glyceraldehyde-3-phosphate dehydrogenase