Integrated Lentivirus Copy Number Quantification

The Lenti-X Provirus Quantitation Kit allows you to rapidly determine the copy number of integrated lentiviruses (proviruses) present in a mixed or clonal population of transduced cells. The kit includes a genomic DNA purification kit, and all the reagents required to conduct 200 SYBR® Green-based qPCR reactions using either the calibrated control template or purified cellular genomic DNA.

Determine Precise Effective Lentivirus Titers

By quantifying the number of lentiviruses that have integrated into the nuclear DNA of your target cells, you can precisely determine the real titer (i.e., effective titer) of your lentiviral supernatant stock and use this information to infect cells with greater precision and more highly defined MOI (multiplicity of infection), allowing you to predict how many viral genomes will integrate into your cells. You can also use this method to calibrate other titration methods which measure virus content in packaging cell supernatants but do not measure functional integrative titers in your target cells.

Predictable and Consistent Lentiviral Transgene Expression

When using lentiviral vectors to deliver transgenes into target cells, expression levels increase with increasing MOI because more lentivirus copies integrate into the nuclear DNA of the cells. Also, for a given virus prep, its effective MOI across different target cell lines can vary widely depending on each cell line’s individual susceptibility to lentivirus infection. The Lenti-X Provirus Quantitation Kit allows you to assess the results of your transductions in any cell line, independent of expression level, and can be used to ensure consistent transductions across different cell lines or when using different virus preparations.

Features

Ensure consistent copy number between experiments or across different cell lines

Reagents for 10 genomic DNA purifications and 200 SYBR qPCR reactions

*The primers included in this kit are designed to amplify a conserved region of HIV-1 genome adjacent to the packaging signal. These primers are compatible with all lentiviral vectors sold by Clontech and most commonly used lentiviral vectors. If you are unsure if this kit is compatible with your vector, please send your vector sequence to tech@clontech.com, and our support team will be happy to confirm primer compatibility.

Applications

Accurate measurement of integrated copy number of lentivirus

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.