Matrices listed below were spiked with certain level of TNFRSF13B/TACI and the recovery rates were calculated by comparing the measured value to the expected amount of TNFRSF13B/TACI in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

85-97

90

EDTA plasma(n=5)

88-104

98

heparin plasma(n=5)

88-105

98

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFRSF13B/TACI and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.