RE: Objective cleaning.

From:

Gary Gill

John:
I'm familiar with this technique. It was introduced in a Leitz technical
bulletin many years ago. I have a copy in my files at home.
Gary
-----Original Message-----
From: J. A. Kiernan [mailto:jkiernan@uwo.ca]
Sent: Wednesday, February 26, 2003 12:24 PM
To: Gary Gill
Cc: 'Ian Montgomery'; histonet@pathology.swmed.edu
Subject: Re: Objective cleaning.
A method for getting oil off an objective is
to take a piece of expanded polystyrene (the
popcorn-like stuff used for packing), break it
in half, and place the lens against the newly
broken surface. Almost all the oil is rapidly
soaked up by the polystyrene. I got this from
a book: Light Microscopic Techniques in Biology
and Medicine by J. James; The Hague: Martinus
Nijhoff, 1976. It works well.
John Kiernan.
Gary Gill wrote:
> Regardless of the solvent used, never put it directly onto a lens surface,
as it may seep around the lens circumference and loosen the cement that
holds the lens in place.
> Instead, first put a drop or two of cleaner on a double thickness of lens
tissue and then clean the lens. For recessed concave surfaces of plano
lenses, use a sharpened bamboo stick to press on the moistened lens tissue
and move it over the lens surface. Move to a dry area of lens tissue, and
thoroughly dry the residual fluid to avoid spotting that can make the lens
dirtier than it was initially.
> Use an inverted eyepiece as a jeweler's-like loupe to inspect the lens
before cleaning, so you'll know what you're dealing with, and after cleaning
to confirm that you've accomplished what you intended.
--
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
kiernan@uwo.ca
http://publish.uwo.ca/~jkiernan/