There are numerous methods to measure aflatoxin levels and no one method is considered standard for testing peanuts. Even among PMIL collaborators, different evaluation methods have been reported in individual studies, making the comparison of results difficult. This project conducted a systematic comparative study to evaluate and report existing/emerging analytical methods for aflatoxin determination in peanuts and peanut products. A blind test, in which the variety of peanut products was naturally and artificially contaminated with aflatoxin, was prepared to test the current available analytical methods within the collaborating institutions/analysis laboratories. Results from the project were helpful to document the existing methods, the advantages/disadvantages of each method, and which method is best for each objective.