@article{10.1371/journal.ppat.1002575,
author = {Copin, Richard AND Vitry, Marie-Alice AND Hanot Mambres, Delphine AND Machelart, Arnaud AND De Trez, Carl AND Vanderwinden, Jean-Marie AND Magez, Stefan AND Akira, Shizuo AND Ryffel, Bernhard AND Carlier, Yves AND Letesson, Jean-Jacques AND Muraille, Eric},
journal = {PLOS Pathogens},
publisher = {Public Library of Science},
title = {In Situ Microscopy Analysis Reveals Local Innate Immune Response Developed around Brucella Infected Cells in Resistant and Susceptible Mice},
year = {2012},
month = {03},
volume = {8},
url = {http://dx.doi.org/10.1371%2Fjournal.ppat.1002575},
pages = {1-18},
abstract = {Author Summary Brucella are facultative intracellular bacteria chronically infecting humans and animals causing brucellosis, one of the most common zoonotic disease worldwide which can result in infertility and chronic debilitating disease. The cells supporting Brucella growth in vivo remain largely unknown. In order to identify these, we constructed a Brucella melitensis strain expressing a fluorescent protein that allowed us to characterize infected cells by microscopy of the spleen and liver from infected mice. In both tissues, the majority of primary infected cells were cells from the macrophage lineage. The peak of infection correlated with granuloma development. These structures contained the majority of bacteria and were mainly composed of cells expressing CD11b, F4/80, MHC-II, which are specific of activated monocytes/macrophages. A fraction of granuloma cells also expressed CD11c and were similar to inflammatory dendritic cells (DCs). During the chronic phase of infection in susceptible mice, we identified a particular subset of DC expressing CD205 and serving as a reservoir for the bacteria. Overall, our results describe the nature of immune cells infected by Brucella in vivo and reveal an unappreciated role for DC subsets, both as effectors and reservoir cells. These results could help develop new therapeutic strategies to control Brucella infection.},
number = {3},
doi = {10.1371/journal.ppat.1002575}
}