An increased expression of nucleolin is associated with a physiological nucleolar segregation

An increased expression of nucleolin is associated with a physiological nucleolar segregation
Alvarez, M; Quezada, C; Navarro, C; Molina, A; Bouvet, P; Krauskopf, M; Vera, M.I
2003-01-31 00:00:00
Nucleolar segregation is the most striking cellular phenotypic feature of cold-acclimatized carp and depicts the cyclical reprogramming that the physiology of the fish undergoes between summer and winter, where a clear differential expression of some nucleolar related genes occurs. We characterized carp nucleolin, a nucleolar protein involved in multiple steps of ribosome biogenesis, and evaluated its expression upon fish acclimatization. We show that the carp cDNA deduced amino acid sequence exhibits the same tripartite structural organization found in other species. Nevertheless, we observed that nucleolin mRNA expression was strongly induced in the cold-adapted carp as was the nuclear protein content, assessed by immunocytochemistry in liver sections. The physiological up-regulation of nucleolin in the cold-acclimatized carp, where rRNA transcription and processing are depressed concomitantly with the nucleolus segregation, is consistent with the notion that nucleolin plays a fundamental role in repressing rRNA synthesis.
http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.pngBiochemical and Biophysical Research CommunicationsElsevierhttp://www.deepdyve.com/lp/elsevier/an-increased-expression-of-nucleolin-is-associated-with-a-rGmhiXgSWR

An increased expression of nucleolin is associated with a physiological nucleolar segregation

Abstract

Nucleolar segregation is the most striking cellular phenotypic feature of cold-acclimatized carp and depicts the cyclical reprogramming that the physiology of the fish undergoes between summer and winter, where a clear differential expression of some nucleolar related genes occurs. We characterized carp nucleolin, a nucleolar protein involved in multiple steps of ribosome biogenesis, and evaluated its expression upon fish acclimatization. We show that the carp cDNA deduced amino acid sequence exhibits the same tripartite structural organization found in other species. Nevertheless, we observed that nucleolin mRNA expression was strongly induced in the cold-adapted carp as was the nuclear protein content, assessed by immunocytochemistry in liver sections. The physiological up-regulation of nucleolin in the cold-acclimatized carp, where rRNA transcription and processing are depressed concomitantly with the nucleolus segregation, is consistent with the notion that nucleolin plays a fundamental role in repressing rRNA synthesis.

Journal

Biochemical and Biophysical Research Communications
– Elsevier

Published: Jan 31, 2003

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References

Prolactin gene expression and changes of prolactin pituitary level during the seasonal acclimatization of the carp