competent bacteria

Gregg B Wells wrote:
>[--snip--]
>> We have very good results with the method described by Inoue, Nijima and
> Okayama, "High efficiency transformation of Escherichia coli with
> plasmids," Gene 96: 23-28 (1990). Calcium and manganese (i.e. Mn2+)
>[--snip--]
This isn't going to say much except to endorse what Gregg said. I was using a
method which gave ~10^7 cfu/ug and the first time I tried the Inoue method (not
even being very careful with it either), I got 5x10^8 cfu/ug. We grew our cells at
25C as well. One good thing is that you get _lots_ of aliquots from a culture of a
few hundred mls of bacteria.
Simon.
--
Dr. Simon Dawson TEL:+44 (0)115 9249924 ex. 44787
Dept. of Biochemistry FAX:+44 (0)115 9422225
Queens Medical Centre Email:Simon.Dawson at .nott.ac.uk
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