Specifications: Cytochrome P450 4A antibody

Full Name

cytochrome P450, family 4, subfamily a, polypeptide 2

Background

The cytochrome P450 (P450) family of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as NADPH and P450 reductase. There are over 200 cDNA’s which encode P450 protein. Epoxygenases are those P450 proteins which metabolize AA to epoxyeicosatrienoic acid (EETs) and w-hydroxylases are those P450 proteins which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). 20-HETE is converted from AA by the 4A family of P450 proteins which includes at least 8 different, though closely related isoforms. 4A1, 4A2, & 4A3 have been cloned from liver, kidney and testis and have been detected in renal, hepatic & brain microvessels.

ICC/IF analysis of H-4-II-E cells with (left) or without (right) Cytochrome P450 4A antibody at a dilution of 1:100 (green). F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a magnification of 60x.

ICC/IF analysis of HeLa cells with (left) or without (right) Cytochrome P450 4A antibody at a dilution of 1:100 (green). F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a magnification of 60x.

ICC/IF analysis of PC12 cells with (left) or without (right) Cytochrome P450 4A antibody at a dilution of 1:100 (green). Nuclei (blue) were stained with DAPI. Images were taken at a magnification of 60x.

ICC/IF analysis of H-4-II-E cells with (left) or without (right) Cytochrome P450 4A antibody at a dilution of 1:100 (green). F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a magnification of 60x.

ICC/IF analysis of HeLa cells with (left) or without (right) Cytochrome P450 4A antibody at a dilution of 1:100 (green). F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a magnification of 60x.

ICC/IF analysis of PC12 cells with (left) or without (right) Cytochrome P450 4A antibody at a dilution of 1:100 (green). Nuclei (blue) were stained with DAPI. Images were taken at a magnification of 60x.