genes PDC, TDC, TAL, SHT, and THT are introduced into engineered Escherichia coli strain HP-2 to synthesize N-(p-coumaroyl) phenethylamine and N-(p-coumaroyl) tyramine, method evaluation and optimization, overview. p-Coumaric acid is synthesized from tyrosine by TAL and phenethylamine is synthesized from phenylalanine by PDC. Therefore, the intracellular concentrations of both tyrosine and phenylalanine are critical for the final yield of N-(p-coumaroyl) phenethylamine

Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

L-phenylalanine

Pseudomonas putida

-

phenylethylamine + CO2

-

-

?

L-phenylalanine

Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440

-

phenylethylamine + CO2

-

-

?

Organism

Organism

Primary Accession No. (UniProt)

Commentary

Textmining

Pseudomonas putida

Q88JU5

-

-

Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440

Q88JU5

-

-

Substrates and Products (Substrate)

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

L-phenylalanine

-

748581

Pseudomonas putida

phenylethylamine + CO2

-

-

-

?

L-phenylalanine

-

748581

Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440

phenylethylamine + CO2

-

-

-

?

additional information

enzyme PDC from Pseudomonas putida is active on L-amino acids and shows higher substrate specificity for phenylalanine than for tyrosine

748581

Pseudomonas putida

?

-

-

-

-

additional information

enzyme PDC from Pseudomonas putida is active on L-amino acids and shows higher substrate specificity for phenylalanine than for tyrosine

genes PDC, TDC, TAL, SHT, and THT are introduced into engineered Escherichia coli strain HP-2 to synthesize N-(p-coumaroyl) phenethylamine and N-(p-coumaroyl) tyramine, method evaluation and optimization, overview. p-Coumaric acid is synthesized from tyrosine by TAL and phenethylamine is synthesized from phenylalanine by PDC. Therefore, the intracellular concentrations of both tyrosine and phenylalanine are critical for the final yield of N-(p-coumaroyl) phenethylamine

Pseudomonas putida

Natural Substrates/ Products (Substrates) (protein specific)

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

L-phenylalanine

Pseudomonas putida

-

phenylethylamine + CO2

-

-

?

L-phenylalanine

Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440

-

phenylethylamine + CO2

-

-

?

Substrates and Products (Substrate) (protein specific)

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

L-phenylalanine

-

748581

Pseudomonas putida

phenylethylamine + CO2

-

-

-

?

L-phenylalanine

-

748581

Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440

phenylethylamine + CO2

-

-

-

?

additional information

enzyme PDC from Pseudomonas putida is active on L-amino acids and shows higher substrate specificity for phenylalanine than for tyrosine

748581

Pseudomonas putida

?

-

-

-

-

additional information

enzyme PDC from Pseudomonas putida is active on L-amino acids and shows higher substrate specificity for phenylalanine than for tyrosine