Abstract

2974

Matrix metalloproteinases (MMPs) are a family of zinc dependent proteins that selectively degrade components of the extracellular matrix (ECM). MMPs can be categorized based on their substrate specificity and sequence characteristics. Our previous study identified elevated expression of 12 MMPs in African American cell lines when compared to their Caucasian counterparts. This study investigates the expression of the 12 potential biomarkers and their regulation by RECK, (reversion inducing cysteine-rich protein with Kazal motifs), in metastatic cell lines. RECK is a membrane-anchored glycoprotein that negatively regulates matrix metalloproteinases and inhibits tumor metastasis and angiogenesis. Metastatic breast cell lines derived from African American and Caucasian patients were assayed to demonstrate alterations in the transcription of the identified biomarkers. Total RNA was isolated from tissue and cell lines. The steady state levels of mRNAs were measured by RT-PCR analysis. An aliquot of 0.25 micrograms of total RNA was used in the RT-PCR assay. From this data, differential expression of the 12 biomarkers and RECK was demonstrated between metastatic cell lines. This, evidence indicates that elevated expression of MMPs in African American breast cancer may correlate to decreased RECK expression and may play a role in the aggressive phenotype seen in African American women.