Secondary Antibodies

Negative Isotype Controls

Useful Reagents

Positive Controls

Histology Controls

References

Reviews

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Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL12 gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L11P family of ribosomal proteins. It is located in the cytoplasm. The protein binds directly to the 26S rRNA. RPL12 is co-transcribed with the U65 snoRNA, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPL12 dispersed through the genome (provided by RefSeq, Jul 2008).

Rabbit anti Human RPL12 antibody detects a band of 21 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.

Target Species

Human

Western Blotting

Anti RPL12 detects a band of approximately 21 kDa in HEK293 cell lysates

Anti RPL12 detects a band of approximately 21 kDa in HEK293 cell lysates

Species Cross-Reactivity

Target Species

Cross Reactivity

Mouse

N.B. Antibody reactivity and working conditions may vary between species.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name

Yes

No

Min Dilution

Max Dilution

Western Blotting

1/1000

Application Name

Yes

No

Min Dilution

Max Dilution

Western Blotting

1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.