Additional file 2.

Nucleotide alignment of known ELA-DQA alleles identified in Equidae. Dots indicate identity to first sequence in alignment, Eqas-DQA*01. E. burchelli alleles are shown in gray. The thirteen novel E. burchelli alleles identified in this study (Eqbu-DQA*09 -*21) are highlighted in dark gray, whereas alleles discovered in previous studies are
highlighted in light gray. One allele (Eqbu-DQA*21) has a frame-shift mutation (~) at position 176.