LeGene One-Step qRT-PCR Master Mix, ROX, 500X 50μl reactions

Price:$1,232.00

Catalog Number:6472-50

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Description

LeGene One-Step qRT-PCR Master Mix, Rox is a 2X concentrated formulation mix. It is designed for the reverse transcription (RT) and
polymerase chain reaction (PCR) amplification of a specific target RNA from either total RNA or mRNA in a single tube for the detection
and quantification of RNA using real-time detection instruments. The proprietary 2X qRT-PCR Master Mix contains an engineered RnaUsScript
Reverse Transcriptase (RNaseH minus), DnaUs Hot Start Taq DNA polymerase, dNTPs, Mg2+, enhancer, and stabilizer.
DnaUs Hot Start Taq DNA polymerase is an antibody-inactivated hot-start enzyme designed to block polymerase activity between ambient to RT
reaction temperature. RnaUsScript RT enzyme can synthesize cDNA at a temperature range of 40-55oC. Once the PCR step reaches denaturation
temperature (95oC), Taq DNA polymerase activity is restored and the resulting PCR exhibits higher sensitivity, specificity and yield.
The system enables highly sensitive detection from as few as 1 copy of a target gene, with a broad dynamic range that supports accurate
quantification of high-copy mRNA from up to 1 μg of total RNA. Sufficient reagents are provided for 500 amplification reactions
of 50 μl each.