International Journal of Molecular and Cellular Medicineمجله بین المللی سلولی و مولکولیInt J Mol Cell MedMedical Scienceshttp://ijmcmed.org1admin2251-96372251-964510.22088/IJMCM.BUMSenjalali139441gregorian20157143online1fulltextenDiversity of Variable Number Tandem Repeat Loci in Shigella Species Isolated From Pediatric PatientsInfectious disease (Molecular and Cellular aspects)Infectious disease (Molecular and Cellular aspects)Original ArticleOriginal Article<p>Multilocus variable number tandem repeat (VNTR) analysis (MLVA) is a new typing method with several advantages compared to other methods. Dissemination of Shigella is highly significant in developing countries. Whilst Shigella is becoming increasingly important as an etiologic agent of pediatric shigellosis in Iran, little is known about the genetic diversity of the local strains. Therefore, the aim of this study was to describe the genetic diversity of Shigella species isolated from pediatric patients in Tehran, Iran. A total of 53 Shigella isolates were obtained from 1070 patients with diarrhea (less than 12 years of age). All isolates were identified by routine biochemical and serological tests. The confirmed Shigella isolates were further serogrouped (by the slide agglutination) using slide agglutination method. MLVA assay with the seven loci resolved 53 Shigella isolates into 36 different genotypes. Almost all the isolates were classified into five clonal complexes. Furthermore, our MLVA assay could effectively distinguish the four Shigella species. This study has provided valuable insights into the genetic heterogeneity of Shigella species in Tehran, Iran. Our findings can be helpful for further epidemiological surveillance of Shigella species in this country in the future.</p>
Shigella, MLVA, genotyping174181http://ijmcmed.org/browse.php?a_code=A-10-409-1&slc_lang=en&sid=1RezaRanjabrranjbarre@gmail.com1003194753284600549910031947532846005499NoMolecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.MojtabaMemarianimemaryani@gmail.com1003194753284600550010031947532846005500Yes
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.HamedMemarianih.memariani@gmail.com1003194753284600550110031947532846005501NoDepartment of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran.