Application Information: hnRNP A1 antibody [9H10]

ELISA: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. This antibody does not IP the hnRNP complex. WB: Use at an assay dependent dilution. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa). Optimal dilutions/concentrations should be determined by the end user.

Form Supplied

Liquid

Concentration

2
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Protein A purified

Purification Note

Purified from tissue culture supernatant.

Specifications: hnRNP A1 antibody [9H10]

Full Name

heterogeneous nuclear ribonucleoprotein A1

Product Description

Mouse monoclonal [9H10] to hnRNP A1

Background

The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for this gene but only two transcripts are fully described. These variant have multiple alternative transcription initiation sites and multiple polyA sites.