Characterizing the microbiome by targeted sequencing of bacterial 16S rRNA and fungal ITS genes

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July 05, 2019

Bacterial communities are everywhere. The microbiome profoundly
affects human, animal and environmental health. The main questions when
investigating microbial communities are which microorganisms are
present, how abundant are they and how are they interacting with their
host or environment?

We demonstrate taxonomic profiling of bacterial and fungal
communities with QIAGEN Microbial Genomics Pro Suite tools, using a case
of decomposing beech leaves.

Decomposition of leaf material is a dynamic process where the
composition of the litter changes continuously. To accommodate
nutritional fluctuations, the microbial community must be diverse and
dynamic. We analyze the changes in the microbiome over time in response
to nutrient availability and discover the interplay between bacteria and
fungi.