Functions of the eyestalk CHH family neuropeptide genes in the shrimp: use of RNA interference to silence genes that suppress reproduction

Functions of the eyestalk CHH family neuropeptide genes in the shrimp: use of RNA interference to silence genes that suppress reproduction

Principal Investigator

Dr Chan, Siu Ming
(Principal investigator)

Duration

36

Start Date

2005-09-01

Completion Date

2008-08-31

Amount

1362914

Conference Title

Presentation Title

Keywords

neuropeptide genes, shrimp

Discipline

Marine Biology,Gene Regulation

Sponsor

RGC General Research Fund (GRF)

HKU Project Code

HKU 7482/05M

Grant Type

General Research Fund (GRF)

Funding Year

2005/2006

Status

Completed

Objectives

To prepare double stranded RNA for several potentially useful genes isolated from shrimp; to introduce double stranded RNA to shrimp by various approaches; to monitor the knock down expression of the gene and study the RNAi effects on the expression of other target genes; to perform in vivo injection of dsRNA for genes that suppress gonad and monitor reproductive promotion.