Recombinant Human CENP-A produced inSF9is a glycosylated, polypeptide chain having a molecular mass of 17,015 Dalton. CENP-A is expressed with a -6x His tag and purified by proprietary chromatographic techniques

Description:

Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENP-A to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies. CENP-A is another important centromeric autoantigen in addition to CENP-B: it has a molecular weight of approx. 20 kDa and is incorporated into centromeric chromatin due to its histone-like properties. CENP-A antibodies are an important marker for correct diagnosis of Scleroderma / CREST syndrome in CENP-B-autoantibody negative patients.

Source:

Sf9 insect cells.

Physical Appearance:

Sterile Filtered liquid formulation.

Formulation:

CENP-A is supplied at a concentration of 0.6 mg/ml in 20mM HEPES buffer pH-8, 100mM sodium chloride, and 6M Urea.