Chromatography

A comprehensive range of high performance optical detectors

With JASCO’s long history of spectrometer development, you will find that each detector is well designed; these next generation detectors employ the latest optical and electronic technologies for high sensitivity, excellent stability and easy maintenance.

UV/Vis
UV-4070/75

PDA
MD-4010/15/17

Refractive Index
RI-4030/35

Fluorescene
FP-4020/25

Circular Dichroism
CD-4095

Optical rotation
OR-4090

MD-4010, MD-4015 and MD-4017 PDA Detectors

The LC-4000 Series includes three PDA detectors. The MD-4017 is a useful replacement for a single wavelength detector, the MD-4015 is the workhorse for UV- visible detection in the range 200 to 600nm and the MD-4010 is designed for high sensitivity and high spectral and time resolution.

The MD-4010 PDA detector offers the highest detection sensitivity, equivalent to a single wavelength detector. Making use of a 1024 element photometric diode array, the measurement wavelength range is from 190nm to 900nm with a MD-4010 PDA resolution of 1nm and fast spectrum acquisition rate of up to 100 spectra/sec. When used with the new temperature controlled SP type semi-micro flow cell the MD-4010 is well suited to the narrower peaks typically found with UHPLC or RHPLC separations. The MD-4010 is fitted with an internal mercury lamp for automatic wavelength calibration.

The MD-4015 PDA detector is the routine detector for conventional HPLC, RHPLC and UHPLC. The sensitivity is almost equivalent to that of the MD-4010 but only covers the wavelength range from 200nm to 600nm using a 512 element PDA for 1nm resolution and with high speed 100Hz spectrum acquisition.

MD-4010 PDA

The MD-4017 detector has a price-point comparable to a typical single wavelength detector and has been designed for the user only interested in the UV region from 200-400nm, the resolution is excellent at 1nm and the maximum spectrum acquisition is 20 spectra per second (20Hz), useful for peak widths as small as 1 to 2 seconds.

When used with ChromNAV 2 CDS the PDA detector offers multi-functional analysis with a variety of functions such as spectral analysis, library search for peak identification, peak purity and multi-wavelength quantitation.

ChromNAV PDA data processing included as standard

Front panel access for easy lamp replacement

UV spectra auto-scan

Automatic spectral scannning can be controlled using ChromNAV, when a peak exceeds a set threshold a scan is executed automatically, sendt to SpectraManager and saved.

Spectral measurement view

Spectral measurement view

Fluorescence detector FP-4020/4025

The FP-4020 fluorescence detector has been designed for the pursuit of extreme sensitivity. Re-designing the optics and electronics from the ground up, as well as removing the effects of ambient temperature with a new temperature- regulated cell body has led to the highest sensitivity with stable detection. The new axially irradiated cell design enables the very highest sensitivity detection. These changes have resulted in a giant leap in sensitivity for the FP-4020 to achieve a signal noise ratio of 2300:1 or higher (based on the water Raman peak). The FP-4025 is an excellent routine fluorescence detector that has a slightly lower specification with a water Raman S/N of 1400:1 .

Both detectors can be used for simultaneous detection at two wavelength pairs; excitation and emission wavelengths can be changed during the acquisition using a time program and automatic spectra scanning can be triggered manually, by time or automatically by threshold. A built-in mercury lamp enables automatic wavelength correction and the front-access design allows easy replacement of the flow cell assembly and light source.

FP-4020 Fluorescence

High sensitivity measurement of Anthracene

Dual wavelenght simultaneous measurement

Refractive Index detector RI-4030/4035

The Refractive Index detectors include the optical system mounted in a precisely temperature-regulated housing to eliminate the influence of changes in ambient temperature. The RI-4030 and 4035 detectors are fully stable just one hour after powering on. The RI-4030 can be used for both analytical and preparative scales. In preparative mode the maximum flow rate is 120 ml/min. The RI-4035 is a dedicated semi-micro RI detector with a micro-volume flow cell designed for use with semi-micro GPC analysis using GPC columns with a fine pore size.

The 100Hz data acquisition rate makes the RI-4035 suitable for RHPLC and UHPLC separations. The LED light source in both detectors has a long life time (over 10,000 hours) for low maintenance operation. Both the RI-4030 and RI-4035 detectors can be fitted with an optional pressure release safety-valve to prevent damage to the flow cell in the event blockage

RI-4030 Refractive Index

Circular Dichroism detector CD-4095

The CD-4095 is a unique HPLC detector for measuring circular dichroism developed from the market leading J-Series circular dichroism spectropolarimeter. Optically active compounds with a chromophore close to a chiral center may absorb circularly polarized light, which can be detected with excellent sensitivity and selectivity. When chiral compounds are measured using a UV-Visible detector, the d- and l- enantiomers cannot be distinguished even when separated by a chiral column.

The CD-4095 circular dichroism detector makes it possible to positively distinguish between the d- and l- enantiomers by measuring the positive and negative peaks. It is also possible to quantify the d- and l-forms even when they are not separated, a calibration curve based on the g-factor can be used to determine the enantiomeric ratio. The ChromNAV CDS can be used to simultaneously measure the CD, g-factor data and UV-visible signal. The CD spectra of eluted compounds can be measured by triggering scanning manually, by timed program or automatically by threshold.

CD-4095 Circular Dichroism

Chiral detector OR-4090

The OR-4090 Chiral detector measures the angle of rotation of plane polarized light caused by optical active isomers and is useful for chiral compounds with no absorption. The OR-4090 uses a high intensity Hg-Xe arc lamp (150W) providing a strong output in the UV/Visible region where the largest optical rotations offer the highest sensitivity. The wide wavelength range from 350-900nm also allows measurement of a wide variety of samples. One of the key benefits of optical rotation detection is that it is unaffected by changes in solvent and can be used with gradient elution, unlike an RI detector.