The SMARTer Universal Low Input RNA Kit for Sequencing provides a simple and efficient solution for generating cDNA for RNA-seq from very low amounts of input RNA that is compromised, has low RIN values, or is derived from a source that does not have polyadenlyated mRNA. The kit works with as little as 200 pg of rRNA-depleted material (derived from 2–100 ng of total RNA). The SMARTer Universal Low Input RNA Kit for Sequencing has been designed specifically for RNA-seq with FFPE samples and laser captured materials. It outperforms existing methods in body coverage, reproducibility, mappability and ERCC correlation. cDNA produced with this kit is compatible with both Illumina® and Ion Torrent low-input library preparation kits.

Applications

Generate cDNA for RNA-seq from very low amounts of total RNA (2–10 ng) from FFPE or LCM samples

Generate cDNA for NGS from very low amounts of total RNA (2–10 ng) that are:

Compromised (obtained from FFPE or LCM samples)

Have low RIN values (<8)

Are from sources that do not have polyadenylated mRNA

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.