Custom antibody development

We offer world-leading antibody development platforms based on a proprietary RabMAb® rabbit monoclonal platform, a phase display platform (AxioMx), and a next-generation sequencing platform (NGS-RabMAb®)

Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

IP

Use a concentration of 5 µg/ml.

ICC/IF

Use a concentration of 5 µg/ml.

WB

Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).Can be blocked with Human SNAP25 peptide (ab41725).

Target

Function

t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.

SNAP25 was immunoprecipitated using 0.5mg Rat Spinal Cord tissue lysate, 5µg of Rabbit polyclonal to SNAP25 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Rat Spinal Cord tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab41726.Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.Band: 26kDa; SNAP25; non specific bands - 55 and 98kDa: We are unsure as to the identity of this extra band.

Thank you for providing the additional information. I see your earlier e-mail in our records.
We have 15 SNAP25 antibodies in our catalogue. Some are polyclonals raised against full-length SNAP25, and since these will potentially recognize multi...