National course open for SciLifeLab facility users and potential facility users, such as PhD students (prioritized), postdocs, researchers and other employees in need of proteomics skills within all Swedish universities.

To attend, the course participants must have background in the fields of biology, medicine, biochemistry or similar.

For questions about the course curriculum, contact: Masood.kamali@igp.uu.se

Social program

The course offers a social program with all lunches and one dinner together with the teachers included (cost TBA). Attendance is recommended, as it provides excellent opportunities for networking, but it is not mandatory.

Social program participants will be sent an invoice for the cost of the food, SEK 2000, after the course has been completed. NOTE – Uppsala University cannot invoice individuals.

Aim

The aim of the course is to introduce scientists at the Disciplinary domains of Medicine and Pharmacy and of Science and Technology and other interested scientists to novel and advanced technologies for proteome analysis offering powerful tools in basic research and diagnostics. The course is intended to facilitate networking between scientists and these proteomics facilities at the SciLifeLab-Uppsala. Both affinity- and non-affinity-based methods for protein detection will be covered. Mass spectrometry will be used for unbiased and high-throughput protein discovery and characterization; different variants of proximity ligation assays (PLAs) will be used for sensitive detection of proteins and their interactions and modifications in solution or in situ; and the participants will be introduced to histopathology methods including tissue microarray production, immunohistochemistry and slide scanning, using techniques, reagents and sample resources from the Human Proteome Atlas project.

Course content

The course will provide insight into state-of-the-art technologies for proteome analyses in research and medicine via advanced molecular technologies, reagent resources and instrumentation. The course will be organized by the proteomics facilities at Science for Life Laboratory, Uppsala, and will convey, by lectures and hands-on training, information about established and emerging methods and technologies relevant in basic research and diagnostics. Affinity- and non-affinity-based methods for protein analyses will be covered.

Topics covered will include:

In situ PLA

Solid-phase PLA

Mass spectrometry

HPA database

Bioinformatics

Pathways analyses

The theoretical moments will include lectures, seminars and discussions, and they will be combined with practical moments including introduction to mass spectrometry, solid phase- and in situ PLA, and immunohistochemistry with tissue microarray production and image analysis.

Entry requirements

The course participants must have background in the fields of biology, medicine, biochemistry or similar.

Date and time

2018-08-27 - 2018-09-07

Location

Husargatan 3, Uppsala

SciLifeLab has been created by the coordinated effort of four universities in Stockholm and Uppsala: Stockholm University, Karolinska Institutet, KTH Royal Institute of Technology and Uppsala University.