The reniform nematode (Rotylenchulus reniformis) has emerged as a major cotton pest in the United
States. A recent analysis of over 20 amphimictic populations of this pest from the US and three other
countries has shown no sequence variation at the nuclear ribosomal internal transcribed spacer (ITS)
despite the region’s usual variability. We investigated this unexpected outcome by amplifying, cloning
and sequencing two regions of the nuclear ribosomal DNA (18S, ITS1) to ascertain whether any
variation occurred within and among populations of reniform nematodes in Alabama, US. Both the
nrITS1 and the relatively conserved 18S region showed a fairly substantial amount of variation among
populations. The identity among ITS sequences ranged from 1.00 to 0.86, while sequence identity at the
18S ranged from 1.00 to 0.948. We conclude that variation does exist in these sequences in reniform
nematodes, and the earlier report showing no ribosomal ITS variation in this pest might have been
caused by preferential amplification of a conserved ITS paralog. Current and future application towards
resistance in cotton varieties to this pest requires reliable information on the molecular variability of the
nematode in cotton-growing areas.

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en

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dc.subject

Ribosomal DNA,

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ITS, 18S,

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Reniform nematode.

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dc.title

Nuclear ribosomal DNA diversity of a cotton pest (Rotylenchulus reniformis) in the United States