Approach:
Use comparative genomic methods, microarray analysis, and co-infection studies to explore pathogenic mechanisms of bacteria associated with the porcine respiratory disease complex and their interactions with each other and swine viruses. Assess the usefulness of selected genes or proteins identified in comparative genomic analyses for DNA-based identification and classification, serological detection of infection, and potentially as vaccine candidates. Strategies for improved heterologous protection will be tested using live attenuated vaccines, as will the use of immunomodulators, such as granulocyte colony stimulating factor (G-CSF), for therapeutic, prophylactic, and metaphylactic use to prevent and combat infectious disease and thus reduce antimicrobial usage to treat clinical and subclinical disease. Investigate emerging and potential zoonotic bacteria that could impact the swine industry, including Methicillin-resistant Staphylococcus aureus (MRSA) and Streptococcus suis for antimicrobial resistance and virulence factors through sequencing and proteomics. Investigations will focus on pathogen strain characteristics and differences, interactions of bacterial and viral pathogens with the swine host and the microbial ecosystems of the pig. Pathology of both zoonotic and endemic bacterial pathogens of swine will be utilized for the purpose of understanding disease pathogenesis and developing effective diagnostic assays.