CD-23 -Reply

We have tested CD23 mAbs from both The Binding Site (clone BU38) and DAKO (clone MHM6). DAKO recommends using their CSA detection system for their antibody, but, in our hands, it works very well following HIER (DAKO's regular TRS in a 96 degree waterbath for 40 minutes) and EnVision+ detection. We dilute the antibody 1:20 for a 30' incubation and 1:200 for overnight.
R. Cartun