The population of Kalambaki consists mainly of descendants of four population groups, PontusGreeks, Greeks of Eastern Thrace, Greeks of Asia Minor (mainly from Cappadocia, whose ancestors spoke Turkish but were Greek Orthodox Christians in religion) and Greeks whose ancestors had been living in Kalambaki already before the Treaty of Lausanne (1923) was ratified.

Every year on January 18, the feast day of Saint Athanasius, a religious festival named the "Kourbani" is held in Kalambaki. It is possibly the Christian equivalent of the Muslim Kurban. During this day a dish consisting of veal in a type of wheat known as pligouri is produced and shared among the participants in the festival.