Fast, flexible performance in fed-batch cell culture

Avoid compromises between working volume and feeding space in your bioreactor

The power to overcome traditional solubility limitations impacting total bioreactor working volume is now available. With Gibco™ EfficientFeed™ A+, B+, and C+ feeding supplements, you can deliver super-concentrated nutrients in a complete, easy-to-prepare, dry-format feed. Maximize without compromise.

Superior performance

Achieve at least 2- to 5-fold increases over batch culture, with a means to reach higher titers using super-concentration flexibility

The EfficientFeed™ + Supplements are designed with the flexibility to super-concentrate chemically defined components up to 3X (i.e., 200 g/L) of original levels while remaining pH-neutral. This is accomplished with nutrient delivery technology that allows reconstitution without pH adjustments (i.e., just add water). By adding different amounts of water during reconstitution, you can concentrate these supplements 2X, or up to 3X, as illustrated below.

Comparison of final volumes for reconstituting a 10 L package of EfficientFeed™ + Supplement.

How to super-concentrate

Weigh out the increased amount indicated here instead of the 1X amount.

Mix for the extended time shown here to help completely dissolve the more concentrated supplements.

Product

Concentration

Dissolution Time

EfficientFeed™ A+

1X (66.4 g/L)

20 min

3X (199.2 g/L)

75 min

EfficientFeed™ B+

1X (52.7 g/L)

20 min

3X (158.1 g/L)

50 min

EfficientFeed™ C+

1X (81.2 g/L)

40 min

2X (162.4 g/L)

60 min

Bioreactor utilization

Avoid compromise

Traditionally, bioreactor space necessitated a compromise: the amount of complete feeding supplements one could add meant a trade-off between initial working volume and feeding space (dilution factor).

EfficientFeed™ + Supplements enable you to deliver up to 200 g/L of needed nutrients in a complete, super-concentrated, pH-neutral feed. The reduction in required feeding space provides you with three ways to optimize your bioreactor output for every run:

Reduce product dilution

Maximize working volume

Increase feeding of nutrients for increased titer

Feeding examples (traditional bolus feeding)

Product

Concentration

% Addition on Culture Days Indicated

4

5

6

7

8

9

10

EfficientFeed™ A+

1X (66.4 g/L)

10%

-

10%

-

10%

-

10%

3X (199.2 g/L)

3.3%

-

3.3%

-

3.3%

-

3.3%

EfficientFeed™ B+

1X (52.7 g/L)

10%

-

10%

-

10%

-

10%

3X (158.1 g/L)

3.3%

-

3.3%

-

3.3%

-

3.3%

EfficientFeed™ C+

1X (81.2 g/L)

10%

-

10%

-

10%

-

10%

2X (162.4 g/L)

5%

-

5%

-

5%

-

5%

Performance

We made it even better

Gibco EfficientFeed + Supplements are designed to provide the same 2- to 5-fold increases in titer possible with original EfficientFeed supplements, plus the flexibility to be super-concentrated up to 3X (or 200 g/L) of original levels while remaining pH-neutral and chemically defined.

In the figure below, we demonstrate an improvement in titer with super-concentrated EfficientFeed™ C+ supplement.

2X concentration of EfficientFeed C+ was added at 15% of working volume

A higher titer was achieved compared to the result from an equivalent amount of original EfficientFeed C at 30% volume of addition

Superior performance

Gibco EfficientFeed + Supplements work even better than the original versions. But how do they perform against other supplements?

As an example, a customer completed a head-to-head comparison in fed-batch cell culture for a DG44 IgG-producing cell line, and demonstrated a superior titer using EfficientFeed C+ supplement over approximately 20 days (peak titer levels shown).

Product quality

The quality you expect from Gibco products

Consistent protein quality delivered.

With EfficientFeed + Supplements, performance doesn’t stop at titer. Product quality is also a critical parameter.

Here we examined the impact of changing from EfficientFeed C to EfficientFeed C+ Supplement on the glycoforms of a target protein and saw no change in protein quality, even when EfficientFeed C+ was super-concentrated.

Minimize risk

Simple preparation helps reduce risk

Our Advanced Granulation Technology™ dry media format provides ease of use while helping to reduce risk by minimizing the steps and adjustments needed to prepare media.

Storage of reconstituted liquid also reduces risk

Typical Closed Systems Connections Required

The ability to store reconstituted EfficientFeed+ Supplements at room temperature, in the dark, for the period of a typical fed-batch bioreactor run adds flexibility to make just one closed-system connection per bioreactor run instead of multiple closed-system connections. This reduces the opportunities for an error that could lead to contamination of a run.

Resources

Learn more about Gibco EfficientFeed + Supplements for use in your fed-batch cell culture processes.