Platinum®Taq DNA Polymerase

Platinum® Taq DNA Polymerase is a hot-start enzyme that has been trusted by researchers for robust, reliable amplification for years. This antibody-mediated, hot-start product provides high specificity for use in everyday PCR applications ranging from cloning to genotyping to mutagenesis.

Platinum® Taq Technology

Platinum® Taq DNA Polymerase is a recombinant Taq DNA polymerase complexed with a proprietary antibody that inhibits polymerase activity at ambient temperatures. Due to specific binding of the inhibitor, Platinum® Taq DNA Polymerase is provided in an inactive form. The DNA polymerase is then activated in a temperature-dependent manner (at 94ºC) during the start of PCR. Once dissociated from the inhibitor, the Taq DNA polymerase regains its full activity.

More Coverage for More Amplicons

Products amplified with Platinum® Taq DNA Polymerase (A); the same products amplified with Competitor DNA Polymerase (B). PCR reactions were run using 1 ng of template DNA and 1.25 units of enzyme in each 50 µL reaction. Annealing temperatures were uniform across the amplicon panel. Amplicons ranged from 300 to 1,400 bp in length, with an average length of 553 bp. Each reaction was performed in duplicate.

A. Platinum® Taq DNA PolymeraseB. Competitor DNA Polymerase

High Specificity and Yield Comparison of Platinum® Taq DNA Polymerase to Competitor Polymerase

The data show a summary of two replicates for each of 40 amplicons, indicating the average specific yield for each amplicon and the standard deviation. Platinum® Taq PCR reactions were performed using 1 ng of template DNA per reaction and the manufacturer’s recommended cycling conditions. Annealing and extension times and temperatures were specific to each primer set. Amplicons ranged from 300 to 1,400 bp in length, with an average length of 553 bp. Data shown here compare Platinum® Taq DNA Polymerase (red) with Competitor Taq DNA Polymerase (green).

The data show a summary of two replicates for each of 40 amplicons, indicating the average specificity for each amplicon and the standard deviation. Platinum® Taq PCR reactions were performed using 1 ng of template DNA per reaction and the manufacturer’s recommended cycling conditions. Annealing and extension times and temperatures were specific to each primer set. Amplicons ranged from 300 to 1,400 bp in length, with an average length of 553 bp. Data shown here compare Platinum® Taq DNA Polymerase (red) with Competitor Taq DNA Polymerase (green).