Synthetic peptide corresponding to Human IL17 (internal sequence). ab79056 was raised against a 19 amino acid synthetic peptide from near the center of human IL-17A.Database link: Q16552(Peptide available as ab190733)

Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

ICC/IF

Use at an assay dependent concentration.

IHC-P

Use at an assay dependent concentration.

WB

Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).

ELISA

Use at an assay dependent concentration.

IHC-Fr

Use at an assay dependent concentration.

Target

FunctionLigand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines (PubMed:8676080).

Tissue specificityRestricted to activated memory T-cells.

Sequence similaritiesBelongs to the IL-17 family.

Post-translationalmodificationsFound both in glycosylated and nonglycosylated forms.

Predicted band size : 18 kDaObserved band size : 18 kDaAdditional bands at : 22 kDa,28 kDa,32 kDa. We are unsure as to the identity of these extra bands.The higher molecular weight bands seen in the Western blot may represent glycosylated IL17.

I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications and species listed on the datasheets. Therefore, any IL17 antibody teste...