BIO 325LQuiz Week of October 27, 2008Name: _____________________________________ TA: _______________________1.List the two steps necessary for E. coli cells to take in foreign DNA (2):-Treatment with Calcium Chloride-Heat ShockIf cells have this ability to take in foreign DNA they are described as being (1):____________competent____________________2.Our plasmid was engineered with three genes.

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Unformatted text preview: List the three (3):-GFP-bla-araC 3. What must the transformed bacteria have in their environment in order to express GFP (1)? Arabinose Which gene produces a product responsible for regulating the expression of GFP (1)? ara C 4. List the two steps required to fracture the membrane and release the contents of the bacterial cells (3): Lysozyme Freeze-Thaw cycle...
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