In this page, we present the disulfide bonds determined by MS2DB++ and 8 other different methods for our dataset. These methods are:
MS2DB+, SVM, CSP, MassMatrix, DISULFIND, PreCys, DiANNA, and DISLOCATE.
While MS2DB+ and MassMatrix are MS-based methods (disulfide bond determination occurs via MS/MS data analysis),
all other six methods are sequence-based methods; thus, the disulfide bonds are determined based on predictive techniques
using protein sequence data.

MS2DB++ determined the disulfide linkages by applying the Shafer rule (one of the information-fusion approaches developed by us
based on Dempster-Shafer theory (DST)) using the disulfide connectivity provided by the following methods: MS2DB+, SVM, and CSP.

As it can be seen in the table below, MS2DB++ outperformed all other methods, finding 16 out of the 17 disulfide bonds listed on
UniProt as the known disulfide connectivity for the 7 molecules analyzed. The only S-S bond missed by MS2DB++ was not found by any
of the other methods either (C91-341 for the molecule FTIII).