Wang Zhijie,Huang Qishan Virus Institute of Medical College in Wuhan University (430071)

Objective:While resisting HSV with the formula of Rhizoma polygoni cuspidate and astragalus,whose effective components are the total saponin and resveratrol,the effect of the mutual action of which is explored on the pharmacodynamics,thereby we conducted the study.Methods:We take ACV as positive control,apply plaque reduction test,and make analysis on the pharmacodynamics in resisting HSV-2 333 strain by combining resveratrol of Rhizoma polygoni and total astragalin under TCM functional mechnism.Result:In the Hep-2 cell system,the ED50 of ACV in inhibiting the hyperplasia of HSV-2 333 strain was 10.85ug/ml.While combining in the ratio of resveratrol and total astragalin 1∶1,the ED50 of direct removal,infection block and hyperplasia inhibition on HSV-2 333 strain was respectively 0.41ug/ml,0.66ug/ml and 0.86ug/ml:their combination indexes(CI) were all less than 1.0,P0.0005,this showed the combination of the ratio was strongest in pharmacodynamics with most marked cooperation.Conclusion:The treatment index(TI) was 100 in ACV resisting hyperplasia of HSV-2 333 strain.It was the best ratio of 1Dm resveratrol/1Dm total astragalin,where the Tis were respectively 248.3,154.2 and 118.4 of direct removal,infection block and hyperplasis inhibition.