The
so called hyper-CEST method promises tremendous potential on
molecule-specific MR imaging using hyperpolarized 129Xe caged in
functionalized cryptophane cages. Here we present a model which allows for an
optimization of the hyper-CEST sensitivity in biosensor applications, by
variation of the xenon concentration in the solution. To evaluate the model
we have performed hyper-CEST measurements on samples with 5 μM and 0.5
μM biosensor concentrations and varied the dissolved xenon
concentration. This comparison shows that 50 nM biosensor concentrations
should be detectable within a volume of 1 ml with high sensitivity.