You don't need to stain the nuclear envelope if you are using image
analysis for the quantitation. Can detect the stained material in the
nucleus and use an erosion dilation software technique to detect the
individual nuclei.
Barry
At 05:16 PM 6/17/99 -0400, you wrote:
>Is there a selective stain for the nuclear envelope? I need to count
>stained "stuff" in the nucleus, so I need a way to delimit the borders of
>the nucleus.
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>THANKS!
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