Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age

14.5 days gestation embryo

Applications

DNA repair studies

Storage Conditions

liquid nitrogen vapor phase

Images

Derivation

MB355 (Polb/p53 double null) is a mouse embryonic fibroblast (MEF) cell line derived from polymerase (DNA directed), beta (Polb)/p53 double null embryos at day 14.5 of gestation. The cells were spontaneously immortalized at passage 5 due to the loss of the p53 gene. The cells are transgenic for lambda LIZ (Lac I/cII).

Comments

MB355 (Polb/p53 double null) is a mouse embryonic fibroblast (MEF) cell line derived from polymerase (DNA directed), beta (Polb)/p53 double null embryos at day 14.5 of gestation. The cells were spontaneously immortalized at passage 5 due to the loss of the p53 gene. The cells are transgenic for lambda LIZ (Lac I/cII).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.
Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:8 is recommended

Medium Renewal: Every two to three days

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Population Doubling Time

20 hours

Name of Depositor

RW Sobol

Passage History

MB355 (Polb/p53 double null) is a mouse embryonic fibroblast (MEF) cell line derived from polymerase (DNA directed), beta (Polb)/p53 double null embryos at day 14.5 of gestation. The cells were spontaneously immortalized at passage 5 due to the loss of the p53 gene. The cells are transgenic for lambda LIZ (Lac I/cII).

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.