Section

linear single stranded molecule-carries codon information for translation-2% of total RNA

mRNA

percentage of tRNA

16%

percentage of rRNA

82%

DNA and RNA synth direction

DNA synthesis: 5' to 3', so the parent strand is read from 3' to 5'. mRNA synthesis: same as above

All oxidoreductases need

a coenzyme. when substrate is oxidized, coenzyme is reduced

Upstream v downstream

Upstream is toward the 5' end of the RNA molecule and downstream is toward the 3' end. When considering double-stranded DNA, upstream is toward the 5' end of the coding strand for the gene in question and downstream is toward the 3' end

Non-Competitive inhib Km, Vmax

Vmax reduces, Km unchanged.

Competitive inhib Km, Vmas

Vmax = same, apparent Km larger (but affinity is the same)

Lineweaver Burk Plot

The intercept with the y-axis is 1/Vmax, the intercept with the x-axis is -1/Km, the slope is Km/Vmax