Family tradition holds that John arrived on the ill fated voyage of the Angel Gabriel in 1635, though there is no direct evidence. He may also have arrived with other Dover Planters in Salem at about the same time. In any event, he is the immigrant ancestor of the New Hampshire Tuttle line, and was established at Dover Neck by 1640.

Notable for founding the longest continuously operating family farm in America. For more information, see Tuttle's Red Barn.

Court Records, 30 Jun 1663: Jn'o Tuttle of Dover dying Intestate, this Court empowers his Widdow Dorothy Tuttle as Administratrix to ye s'd estate who brought into Court an Inventory of his estate amounting to 85:19:6 w'ch the Court ord'r as followeth: viz't It appearing to this Court y't the Eldest daughter of the deceased is maried & hath had her portion already[;] ord'd that his Son Jn'o Tuttle shall have 10:0:0 when he comes to 21 yeeres of age & ye youngest daughter to have 15:0:0 when she coms to the age of 18 yeeres, or be disposed of in marrieg & the remainder of ye estate shall be to ye Widdow during her Life or Widdowhood estate & if in Case she shall marry then to have the thirds according unto Law; & after ye widdows decease or marriage the Son to have the Lands.