Procainamide: A reliable and flexible approach to glycan analysis

Procainamide labeling permits glycan identification by either mass spectrometry or (U)HPLC, and because of its improved ionisation efficiency compared to 2AB labeling it can permit identification of minor glycans (<1% relative peak area) by ESI-MS.

Ludger's procainamide labeling system is suitable for N-glycans, O-glycans, GSL-glycans, heparin or any sugar with a reducing terminus. Also, once labelled with procainamide the glycans can be incubated with exoglycosidases.

As well as labelling kits we offer a clean up system for use after labeling and a range of procainamide labeled glycan standards (including an N-glycan library and glucose homopolymer ladder). Using products together (including LudgerZyme PNGaseF, LZ-rPNGaseF-kit), N-glycan sample preparation can be carried out in one day (for 96 samples)

The Table below outlines the features and benefits of Ludger's procainamide labeling technology in comparison to competitor offerings:

* rapid/instant labelling technologies use N-hydroxysuccinimide (NHS) activated fluorophores that react with the glycosylamine form of the glycan** aminoquinoline labels are not compatible with Bovine Kidney Fucosidase (BKF)