I have used Pfu with great success in amplifying segments up to 2.5 kb with no
loss of fidelity. This also worked with high G-C regions (80%)
Tony Sanchez
University of Florida
Dept. Ophthalmology
1600 SW Archer Road
JHMHC #100284
ARB R4-146
Gainesville, FL 32610
Phone (904)392-2720
Fax (904)392-7839
e-mail = tsanchez at eye1.eye.ufl.edu