LentiFlash®: an RNA delivery lentiviral particle

A short-term expression of your sequence of interest without retro-transcription nor integration

A transient RNA expression without genomic trace

A safe and efficient RNA delivery into any cell type

LentiFlash® particles are VSV-G pseudotyped and highly purified. These two criteria make LentiFlash® very efficient for the transfer of biologically active RNAs into various cell types including immortalized, primary and stem cells.

As shown figure 1, LentiFlash® can transduce at a high level human primary T cells, a clinically target which is not easily transfected using electroporation or chemical transfection methods.

Additionally, LentiFlash® does not require any selection process and preserves the original cell phenotype, its proliferation ability and a full viability.

A transient and fast expression:

LentiFlash® delivers mRNA directly into the cytoplasm leading to a high, fast (as early as 4h post-transduction) of your sequence of interest. These assets make LentiFlash® particularly suitable to carry CRISPR-Cas9 into any cell type while preserving original cell phenotype and viability. RNA delivered by LentiFlash® technology is rapidly bioavailable. (Figure 2)

An RNA-delivery system based on a bacteriophage coat protein

LentiFlash® is an RNA delivery system based on a bacteriophage-lentivirus chimera. It has been constructed by exploiting a bacteriophage coat protein and its cognate 19-nt stem loop instead of the natural lentiviral Psi packaging sequence to achieve mRNA packaging into the lentiviral vectors.

Figure 4: LentiFlash production process

Applications of LentiFlash® technology

Thanks to the high and short-term expression of the transferred RNA LentiFlash® is particularly suitable for: