Access to lands managed by Big Cypress National Preserve south of Interstate 75, from mile marker 59 to mile marker 75 (Turner River Road), south to Upper Wagon Wheel Road is temporarily restricted as well.

The closure also extends to lands administered by Big Cypress National Preserve north of Interstate 75, from mile marker 59 to mile marker 70, north to the Big Cypress Preserve Boundary.

This area includes portions of the Florida National Scenic Trail from mile 12, traveling north to the northern boundary of Big Cypress National Preserve.