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Disease

colorectal carcinoma

Age

43 years

Gender

female

Ethnicity

Asian

Applications

This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985.

The cells and tumors formed from the cells produce detectable amounts of Dopa decarboxylase and the TAG-72 antigen.

Storage Conditions

liquid nitrogen vapor phase

Karyotype

modal number = 46; range = 44 to 48. This is a pseudodiploid human cell line. The modal chromosome number is 46, occurring at 86%, with polyploidy at 16.2%. The ins(1;?)(p13;?), del(3)(p23), t(4q,?), t(8;?), del(9)(p13), del(11)(p13), 14q+ and two other marker chromosomes are common to all metaphases examined. Other altered chromosomes, if any, occur only sporadically. Normal N11 is absent. Two copies of X chromosomes are found in each cell. The Y chromosome is not found in QM stained preparations.

Images

Derivation

This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985.

The line was obtained from a third passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy.

Clinical Data

The line was obtained from a third passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy.

(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)

Comments

This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985.

The line was obtained from a third passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy.

The line was initially grown in RPMI 1640 medium with 20% fetal bovine serum, but was later adapted to growth in the chemically defined medium ACL-4 (it can also be grown in medium with 10% serum).

The cells and tumors formed from the cells produce detectable amounts of Dopa decarboxylase and the TAG-72 antigen.

Complete Growth Medium

ACL-4 medium supplemented with 10% FBSThe base medium for this cell line is ATCC-formulated DMEM: F12 Medium Catalog No. 30-2006.
To make the complete growth medium, add the following components to the base medium:

0.02 mg/ml insulin

0.01 mg/ml transferrin

25 nM sodium selenite

50 nM Hydrocortisone

1 ng/ml Epidermal Growth Factor (do not filter)

0.01 mM ethanolamine

0.01 mM phosphorylethanolamine

100 pM triiodothyronine

0.5% (w/v) bovine serum albumin

10 mM HEPES

0.5 mM sodium pyruvate

2mM L-glutamine(for final conc. of 4.5 mM)

10% FBS

Subculturing

Protocol: Cultures can be maintained by scraping off the attached cells and transfer along with the the floating cells into new flasks.

Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells

Permits

These permits may be required for shipping this product:

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.