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All of the B vitamins are needed for a healthy, functioning nervous system and several are needed to synthesize serotonin (16).

Here are a few that are integrally connected to serotonin production.

Within a few months, this inevitably causes a cascade of negative side effects (10). Datis Kharrazian, author of , lists these co-factors as needed for the synthesis of either tryptophan or 5-HTP to serotonin — vitamins B3, B6, and B12, folic acid, and magnesium.

Serotonin is a neurotransmitter that plays a large role in mood, learning, appetite control, and sleep.

Before you take any serotonin supplement, it’s important to understand a little bit about them and especially how they work together.

Sometimes there are vital synergistic relationships between these various serotonin boosting substances.

Three of the most widely used serotonin supplements are the amino acids tryptophan, 5-HTP, and SAM-e.

Note that it is not considered safe to mix any of these amino acid supplements with antidepressant medications (4).

5-HTP (5-hydroxytryptophan): 5-HTP could be considered as either an amino acid or herbal supplement.

It comes from Griffonia simplicifolia, an African woody shrub. But we’ve put it in the amino acid category since it’s so closely associated with tryptophan. S., Canada, and the United Kingdom, 5-HTP is sold as an over-the-counter supplement for ADHD, anxiety, depression, fibromyalgia, migraines, sleep disorders, and weight loss (7).

A review of 108 studies on 5-HTP revealed there is still no definitive evidence that it helps depression (9). An Ongoing Debate: There’s an ongoing discussion in the natural health community whether it’s better to take tryptophan or 5-HTP to increase serotonin levels.