Tileomatic: Design of oligonucleotide arrays

What is Tileomatic

Tileomatic is a software to design optimal spaced oligonucleotide tiling arrays. Tileomatic balances the three main conflicting objectives in tiling array design—oligonucleotide probe spacing, probe quality and hybridization conditions—to arrive at a globally optimal solution. It is most effective for spaced tiling arrays where variations in spacing can reduce variations in hybridization conditions and avoid having to use low-quality of cross-hybridizing probes. Candidate oligonucleotide probe sets are pre-computed with our OSProbes software

How do I use Tileomatic?

Tileomatic is implemented in a webservice at http://tileomatic.molgen.mpg.de/ It works in a two-phase process: Candidate oligonucleotide probe sets are pre-computed with our OSProbes software. As this requires a considerable computational effort for large genomes, we make a range of model-organisms and candidate sets available; you can request addition of candidate sets with different parameters or for difference model parameters. Given a candidate set, the final array can be efficiently computed with our algorithm in the second phase in two easy steps if you select the new array design menu.