BIOL 429 DISC 6 - BIOL 429 Paper 6 1 What is phos pholipase...

BIOL 429Paper 61. What is phospholipase D and why is it proposed to be involved in receptor-mediated endocytosis?RESULTS 12. What is BtOH and how is it used to “disrupt” PLD effects within the cell?3. Why is PA production used to assay for PLD function in Figure 1?RESULTS 24. How does BtOH affect receptor levels in the cell?RESULTS 35. What two different aspects of receptor-mediated endocytosis are investigated in Results 3?RESULTS 46. Distinguish between RalA WT and RalA S28N.7. How does overexpression of RalA WT affect removal of the receptor from the plasma membrane?RESULTS 58. Why does Go6976 inhibit EGF-induced PLD activity?Phospholipidase D (PLD) is an enzyme that hydrolyzes phosphatidylcholine to form phosphatidic acid and choline. PLD has been implicated in the formation of vesicles, cytoskeletal dynamics and membrane trafficking. The hypothesis that PLD might play a role in receptor-mediated endocytosis accompanied studies of epidermal growth factor (EGF), which is endocytosed upon reception at the cell surface.

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