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Follow the strain isolation technique on Let's Grow Mushrooms, and then select sectors early which are brown in color. By the second or third transfer, you'll see stones developing, and this is only about 1 month after the original swipe of spores on agar.

Now, take each stone and move it to a new dish. Soon, the mycelium will grow out and you'll see fresh stones developing, and if they're good strains, the sclerotia is forming long before the mycelium reaches the edge of the plate. Pick strains which form four or five stones within two weeks and use these for your grain masters.
RR [/quote]

i had some two year old syringes given to me by a friend that had gone through long periods with no refrigeration, so i did some pf jars with them just to try it out. the first round of jars went fairly slowly, but they all fully colonized and i still got some good prints that gave some good mushies in preceding generations. if you do it you'll probably need to be extra patient. the only ones i got to fruit were one's i had cased or dunked prior to their first flush. i think they just took so long to colonize that they lost too much water. I'd go on the heavier side of the water in the initial formula if you're going to do it in jars.

--------------------It used to be that we loved little children. Now we eat them.
--Ira Hyde