Thank you, Sharon Pok from the Australian National University Medical School, for a great review on our ISOLATE II RNA Mini Kit.

"I recently used the ISOLATE II RNA Mini Kit. I had some cultured cells which are derived after FACS, which are not very many, to extract RNA from. Because I had a small starting quantity (<1E6 cells), I wasn’t expecting an extremely high yield. However, I attained a relatively good yield from that small amount of starting sample. What also impressed me was the 260/280 and 260/230 ratios. I have been having trouble getting a good 260/230 ratio lately, probably because of residual guanidine not being washed off properly. With the kit, both ratios were well above 2.0. Waiting to run the samples on the Bioanalyzer to obtain RIN values."