Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry. Between each step, cells were washed with 0.1 % BSA in PBS. Flow Cytometry: 0.1 mg/mL. Use 10 μL to label 1-2.5x10^5 cells in a total volume <200 μL. The binding of unlabeled primary antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of IgG (FITC) xGt.