FAQ: What should I take into consideration when designing a set of PCR primers?

1. Avoid complementarity between the primers to prevent primer-dimer formation.
2. Avoid inverted repeats (self-complementarity).
3. GC-content of the primer should be around 50%.
4. Avoid Gs and Cs at the 3'-end of the primers.

There are many computer programs which will help you to design a primer pair, such as Primer3.

Luna® Universal qPCR & RT-qPCR Products

Introducing a bright, new choice for your qPCR and RT-qPCR. Luna products have been optimized for robust performance on diverse sample sources and target types. Available for dye-based or probe-based detection, Luna products can be used across a variety of instrument platforms. For a limited time, try a free sample of our Luna qPCR or RT-qPCR products and share your data to receive a free Luna T-shirt!