Re: antibody search

Hi Patsy,
Several months back I was also looking for information
on this particular antibody on the net. It isn't very
common and I can tell you from experience not an easy
one to do. The rate of repair is tremendously fast
and measuring it is very difficult. We were using
induced rats (ferric -NTA) and examining both their
kidney and brain tissues. Also there isn't a true
control tissue (only artificially induced) to use with
it either, we did try normal rat kidney and our
results were mixed. I sort of feel that the antibody
wasn't all it was cracked up to be, personally. The
second one we tried had better information and we will
be trying it in the future with I hope better results.
Fixation was also a matter that had to be looked at.
We did both Bouins and 10%NBF. Times were kept to no
more than 10 hours and this too became a critical
factor.
I've used two companies in this study, one was from
Japan and I have misgivings about it's QA/QC. There
is an article written by a Dr. Toyokuni that we tried
to reproduce in our lab with little success. It is a
monoclonal ab and the clone is N45.1, it is made by
the Nippon Food company in Japan and marketed by a
company called "Genox or Gennex" I think.
Unfortunately I'm doing this from home and memory (or
actually nightmare's I had when doing it) so I may not
have the distributors name absolutely correct. When I
get to work in the morning I'll get all of it and send
it.
The second company is: QED Bioscience Inc.
1-800-929-2114. "DNA & RNA Oxidative Damage Markers
Monoclonal Antibody"
Specification Summary:
Antigen: 8-hydroxyguanosine (oh8G)- BSA and casien
conjugates
Host species: mouse
Specificity: recongnizes 8-hydroxy-2'deoxyguanosine
and 8-hydroxyguanosine. They are markers of oxidative
damage to DNA and RNA.
It is stable for at least one year at -20C.
This one has had some better references from it as
well.
A third maker of this is from Columbia University in
NYC and I will also forward this in the next e-mail.
However, as far as I know it only works in frozen
sections.
I will cc the listserver as well with this information
for anyone else who wants to see it.
Best of luck!
If you have any questions I can be reached at
1-914-789-7127 from 9am to 5pm (EST), and I'll be glad
to give you any information that way as well. I owe
you a few favors for your help with my daughter.
Sincerely,
Vikki Baker
American Health Foundation
Valhalla, New York
--- Patsy.Ruegg@UCHSC.edu wrote:
> looking for:
> antibody to 8-Hydroxy-2'-deoxyguanosine.
>
__________________________________________________
Do You Yahoo!?
Yahoo! Mail - Free email you can access from anywhere!
http://mail.yahoo.com/