To assess the effect of multipotent stromal cells (MSC) on monocytes, 3-day cultures were performed of freshly isolated monocytes in MSC-conditioned medium (CM). As a control condition, monocytes were stimulated with low dose macrophage ...

To assess the capacity of multipotent stromal cells (MSC) to induce the generation of Tregs, transwell co-cultures were performed as well as cultures with MSC-conditioned medium (CM). In short, peripheral blood mononuclear cells (PBMC) were ...

Hua Zhang

Southern Medical University

Hello, could you tell me how to Keep PBMC's activity in vitro for almost 7 days without any stimulation? I mentioned that Melidf, S.M. just culture PBMC alone in RPMI medium (containing P/S, L-glutamine and 10% FCS) after co-cultured with MSC in the reference at the end of this protocol. I have tried to culture it alone, but after 7 days of culture its activity (the proliferation of CD4+ T cells) proved to be very weak by stimulating with allogenic cells.