The final week of February, 2019 (Feb. 25 - Mar 1), iLab Operations Software will be conducting an internal event and will be staffed at significantly reduced levels. iLab Support will prioritize critical issues first (i.e. issues that prevent key workflows from completing) and to escalate an issue, please add "Urgent" to the ticket/ email subject or press "1", when prompted, on the iLab Support phone (regional numbers can be found here). Regular support tickets will be monitored periodically and processed in order of submission starting March 4th, 2019. During this time, you can also refer to the iLab Help Site and iLab Status Page for site information. Please note that this will not affect standard Core Facility Operations.

Gene Targeting/Gene Modification/Transgenic Mouse

Overview of Services

Gene ModificationThe Gene Modification Facility is offering services for gene modification in cell lines and in conjunction with the Gene Targeting and Transgenic Mouse Facilities for the complete generation of gene targeted mouse lines including the generation of conventional gene knockouts, conditional knockouts, knock-in mutations and the generation of tagged proteins by conventional homologous recombination based gene targeting strategies or CRISPR (clustered regularly interspaced short palindromic repeats)-mediated gene targeting strategies. CRISPR can shorten the time frame of gene targeted mouse production remarkably and the gene targeted mice can be generated as fast as 2-4 months. Services for each individual step involved in the generation of gene targeted mouse lines are also offered.

Gene TargetingThe Gene Targeting Facility is a centralized facility for the production of gene-targeted mice. The services provided by the facility allow researchers to modify the mouse genome through methods of gene targeting or BAC transgenesis in embryonic stem (ES) cells and to introduce these changes into the mouse genome by the generation of targeted ES cells and chimeric mice. The facility also works in conjunction with the Gene Modification Facility to generate knockout mice.

Transgenic MouseThe Transgenic Mouse Facility trains and assists Einstein investigators in the production and characterization of transgenic mouse strains. The personnel have more than a decade of experience providing high-quality service with minimal waiting times. The facility offers a wide array of mouse embryo-based services for the college, including traditional pronuclear and lentiviral injections as well as consulting, construct design and screening strategies.