FastPfu FLY Ultra High-Fidelity DNA Polymerase

TransStartFastPfu Fly DNA Polymerase is a hotstart, ultra-high fidelity and high-processivity DNA Polymerase. It consists in a modified DNA Polymerase, increasing its affinity for dsDNA and enhancing processivity and other caracteristics. Its fidelity is 108 times higher than EasyTaq DNA polymerase making it the DNA Polymerase with the highest fidelity on the market. In addition, its extension rate reaches 6 kb/min. Compared with FastPfu DNA Polymerase, The FLY version of FastPfu DNA High-Fidelity Polymerase has a faster extension rate, higher fidelity, and greater amplification efficiency over regular FastPfu, making FastPfu FLY the best performing DNA polymerase for ultra low non-specific amplification from complex and unpurified gDNA samples, as well as other difficult PCRs targeting GC-rich or AT-rich DNA sequences. FastPfu FLY is available for NGS library prep amplification.

Published on Jan 30, 2017 by Simon Roy, Ph.D Detection Limit of High-Fidelity DNA Polymerases using Human gDNA for Template We previously determined the detection limit of different Taq DNA Polymerases using human genomic DNA as a template for PCR. For this instance,...