The main core promoter-binding element in polymerase II transcription machinery is

The main core promoter-binding element in polymerase II transcription machinery is TFIID a complex comprising TBP the TATA box-binding protein and 13 to 14 TBP-associated factors (TAFs). choice the nucleotide structure is less essential than the amount of the DNA for high affinity binding. Comparative manifestation profiling of wild-type and a DNA-binding mutant of TAF4 exposed common primary promoter features in the down-regulated genes that add a TATA-box and an Initiator. Additional study of the PEL98 gene out of this group demonstrated reduced Initiator activity and TFIID occupancy in TAF4 DNA-binding mutant cells. These results claim that DNA binding by