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The game boasts 305 characters (56 of which are fully playable) from 41 different Shonen manga series compared to 160 characters (34 fully playable) from 27 different manga in the original. However, the characters from Hikaru no Go and Mr. Fullswing do not return in this sequel (Steel Ball Run is unaccounted for because it has been revised into a story arc within JoJo's Bizarre Adventure between the releases of both games).