F18A12.8 encodes two isoforms of a neprilysin; neprilysins are thermolysin-like zinc metallopeptidases, found on the outer surface of animal cells, that negatively regulate small signalling peptides (e.g., enkephalin, tachykinin, insulin, and natriuretic peptides) by cleaving them; F18A12.8A is closely paralogous to ZK20.6, and orthologous to CG9761 in Drosophila melanogaster; F18A12.8 is homologous, and possibly orthologous, to human ECE1 (OMIM:600423; mutation leads to Hirschsprung disease, cardiac defects, and autonomic dysfunction); more generally, F18A12.8A falls into a group of proteins that includes the classical neprilysins found in mammals (e.g., PEX [OMIM:307800] and the enkephalin cleaving enzymes).

Evidence for Inhibition

ePCR

Status: Unique_ePCR (II:3421852..3422970)
Transcript overlap: 829 nt

Sliding Window

Raw score: 766
Relative score: 1 (This gene has the best score for this RNAi reagent)
Specificity index: 1 (RNAi reagent is specific for this gene)