Usage & Assay Information

Specific activity: approximately > 0.6 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 uM NADPH per minute at 25 DEG C.

Storage & Safety

Storage

Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance

AKR1C4 belongs to the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. It catalyzes the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. Recombinant human AKR1C4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.