I have E. coli with transformed plasmid on agar in a vial. It has been stored at -20 degree C without glycerol stock for 18 hours.
This is a continuation of: Survival of E. coli on agar plate at -20 ...

Quorum sensing is a system of stimulus and responses correlated to population density that is used by bacteria to coordinate gene-expression. I am looking for a simple computational/mathematical model ...

If experiments like those of Luria and Delbruck on E. coli and T1 phage are the main source of our confidence in the mutation-immunity model, is it then highly unlikely that there are other types of ...

Typically, when performing gene-knockout, the experimenters select one gene to remove/replace-with-junk and then see if the prokaryote can still undergo fission. If it continues to reproduce then the ...

I was reading through the Karr et al. (2012) whole-cell computational model. One of the things they did was to induce single-gene disruptions in their model. They observed several to be fatal, but:
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Multidrug resistant probiotics are often recommended by doctors in various cases. But since bacteriae can easily exchange genes by conjugation or other means they could promote the drug resistance of ...

I am trying to reproduce E. coli interrupted mating using an HFR strain, and I read that the cultures should be vigorously shaken at times to interrupt the DNA transfer from the F+(HFR) donors to the ...

In the case of mammals like giraffes and koalas, is that bacteria common on the plants they eat so when a baby starts to try to stick something besides its mother's milk in its mouth, it can't digest ...

Pretty much this. I've been wondering if any of the yogourt and other "health" foods containing living probiotic cultures survive digestion to populate our intestines? If so, is there peer-reviewed ...

Many DNA isolation and protein expression protocols contain instructions to use a starter culture of E. coli that is then used to inoculate the main culture.
What are the advantages of using starter ...

Occasionally, in hospitals and in eating establishments in the US, they have industrial grade UV lights in sconces attached to the wall (though they seem to be less prominent as the years go by). I ...

From my basic understanding, antibiotics kill living things, bacteria for example.
Do the antibiotics consumed by a human-being distinguish between what they kill? Or do they just kill every bacteria ...

Clearly, a zygote does not harbor any microbes. As it develops, and the alimentary canal tissue is differentiated, I logically assume that there is still no microbial activity in the fetus's gut. I'm ...

From all accounts, it seems as if the Escherichia, Enterobacter, etc. that live and thrive in the human gut are pretty well entrenched. I know that these microbial populations are often analyzed as ...

I've read that the amoeba is capable of learning. Since these protists have no nervous system, it's safe to assume that even highly simplified learning mechanisms of the Aplysia are miles off in the ...

I've gotten a staggering amount of use out of my copy of Fields Virology as a general reference for "getting me up to speed" on whatever pathogen I'm currently looking at. I don't know of a similar ...

So I'm looking for some information on the infectious dose necessary to colonize a human with Clostridium difficile. There's no human challenge studies, and since it's not a foodborne pathogen, little ...

Once upon a time, I chanced upon an old microbiology book that detailed the rather colorful world of enterobacteria. Salmonella in particular stood out, as it seemed there were a lot of species: typhi ...