Materials and Methods

Primary culture of maxilla and iliac crest BMSCs from four normal healthy volunteers were expanded in culture. In 24-well plates, first passage BMSCs were seeded directly (1 × 104 cells/well) on oxidized titanium discs (1.27cm diameter and 2mm thickness) or tissue culture plate. Each cell type was assessed for affinity for titanium, post-attachment survival and osteogenic differentiation based on alkaline phosphatase and osteopontin expressions.

Results

There was no difference in the affinity of maxilla and iliac crest BMSCs to titanium. However, titanium-attached maxilla BMSCs were apparently more osteogenically responsive than iliac crest cells based on calcium accumulation and gene expression of alkaline phosphatase and osteopontin. But these differences were not statistically significant in this small patient sample.

Conclusion

Maxilla and iliac crest BMSCs have similar attachment affinity for titanium. This pilot study indicate that titanium-attached maxilla BMSCs were more osteogenically responsive and may be a viable and more readily available donor graft material in implant dentistry.

Copyright/Permission Statement

This is the peer reviewed version of the following article: [Akintoye, S. O., Giavis, P., Stefanik, D., Levin, L., & Mante, F. K. (2008). Comparative osteogenesis of maxilla and iliac crest human bone marrow stromal cells attached to oxidized titanium - a pilot study. Clinical Oral Implants Research, 19(11), 1197–1201. http://doi.org/10.1111/j.1600-0501.2008.01592.x], which has been published in final form at [http://dx.doi.org/10.1111/j.1600-0501.2008.01592.x]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving [link to http://olabout.wiley.com/WileyCDA/Section/id-820227.html#terms].