Summary

Secondary Antibodies

Negative Isotype Controls

Useful Reagents

Positive Controls

Histology Controls

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References

Reviews

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Rabbit anti Human BMP-2 antibody recognizes the disulphide-linked homodimeric cysteine knot protein known as human Bone Morphogenetic Protein 2 (BMP-2/BMP-2a), full-length 396 amino acids. BMP-2 is a member of the Transforming Growth Factor beta (TGF-B) superfamily and one of a growing number of osteogenic proteins shown to induce bone and cartilage formation and to play an important role in developmental processes, including cell proliferation, differentiation, apoptosis and morphogenesis.

BMPs act through binding with a receptor complex consisting of type I and type II serine/threonine kinases, resulting ultimately in the activation of the Smad protein and mitogen-activated protein kinase (MAPK) signaling pathways. Several antagonist proteins, including, noggin, chordin, gremlin and follistatin, are responsible for modulating the signaling effects of BMPs, through the binding and blocking of receptor ligands, thereby preventing activation.

The realization that BMP-2 was involved in the stimulation of bone formation emerged from research in spinal surgery, following the discovery that the healing of bones was directed by proteins contained within the bone matrix itself.

Intended Use

Target Species

Human

Product Form

Purified IgG conjugated to Biotin - lyophilised

Purified IgG - liquid

Reconstitution

Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. N.B. For long term storage the addition of 0.09% sodium azide is recommended.

Preparation

Antiserum Preparation

Antisera to human BMP-2 were raised by repeated immunisations of rabbits with highly purified antigen.

Prior to reconstitution store at +4oC.After reconstitution store at -20oC.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Store at +4oC or at -20oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice

Technical Advice

Recommended Protocol

Recommended Protocol

ELISA

This biotinylated human BMP-2 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human BMP-2 antibody (AHP960) as the capture reagent.

ELISA

This purified human BMP-2antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human BMP-2 antibody (AHP960B) as the detection reagent.

Immunohistology

Immunohistology

Histology Positive Control Tissue

Histology Positive Control Tissue

Immunofluorescence

Immunofluorescence

Western Blotting

This antibody may be used in Western Blotting under either reducing or non-reducing conditions.

Western Blotting

This antibody may be used in Western Blotting under either reducing or non-reducing conditions.