"We determine mainly protein concentrations after purifications in a high throughput manner (200 to 500 samples a week). Data determined with DropSense turned out to be highly reliable and reproducible, while subjecting only 2 to 4 µL to the device plates.
Additional value is given by cDrop application protocols which allow for example determination of specific impurity species on your sample background. In this context, Trinean and we developed a special protocol for one of our applications which turned out to be very helpful and was published. The friendly collaboration was a great base of this successful development.
I rated value for the money we spent with 4 stars only (not 5), which might be due to the fact that the device definitely is quite expensive in comparison to other devices allowing determination of protein concentration. If our work load will increase and we will need more diverse and more complex analysis than we are apparently are running on DropSense, then it would be worth 5 Stars.
"

"The DropSense96 is used for concentration measurement of both antibodies and antibody-drug conjugates at AbbVie. The plate based analysis affords a very high-throughput with a 96-well plate measured in less than 10 minutes. Plate preparation is straightforward and done at the same time as plates for HIC and SEC as no dilutions are required. By using the disposable plates there is no cross contamination. Unused locations on a plate can be used in subsequent analyses so it is possible to use the entire plate which keeps running costs down. The system only uses a very small amount of material, 2μL. The software is intuitive and the PC can be networked. It is easy to generate a standard curve to obtain extinction coefficients. Data can be exported in multiple formats including XLS, CSV and TXT."

Reliable results, with a lot of information of the measured sample what is a great help for our experiments.

Rating: 4.0

Application Area:We use the Trinean Dropsense96 to determine the DNA concentration. Using the cDrop program, we can c

"We use the Trinean Dropsense96 most of the time with the Hamilton Starlet (pipetrobot). The DNA concentration measurement is automatized what makes it works easy, fast and reliable. After the measurement it is possible to see a profile of the DNA sample. In this profile we can see the gDNA, Nucleic Acids, impurity's and turbidity. This gives us the opportunity to optimize our DNA isolation, or to do purification before starting our experiments. The Trinean Dropsense96 is very stable with a good maintenance every year from the company."

"This is a fantastic tool and QC platform for biobanks. We focus on DNA extraction and pre-analysis QC. We don't perform any downstream analysis ourselves but ship normalized DNA to third party laboratories and therefore we need as much QC information as possible. As a high throughput laboratory, our QC must have both a reasonable analysis price and be quick and easy to use on a variety of samples.
This full platform combines the best of both nanodrop and picogreen worlds and can be done with as little as 2µl of sample. The system is very powerful and gives a tremendous amount of data. Software is easy to use but you have to play around with it to learn which buttons to use and which to leave alone to be able get the info you need. There are a lot of parameters and variables so the interface may look a bit chaotic at first.
Be aware that the system comes with the DropQuant software and you need the cDROP add-on to be able to get the full features. However, it is very well worth it as this expensive add-on gives you a very powerful software. The results are very comparable to picogreen (dsDNA) results. It also looks like the reliability is very good as I have got nothing but good feedback from our third party laboratories.
What initially may feel like a little drawback with such a powerful tool as cDROP is that there is no such thing as one universal software protocol that can be used on all samples. If you want to use the complete set of cDROP features the software needs a kit/source specific protocol. Software comes with a lot of pre-installed protocols but your specific combination of source and kit may not be there. Luckily, the protocol developers at Trinean are very helpful so in the end this is no problem or drawback at all. However, nucleic acids isolated with self-established DNA extraction protocols or so called "quick and dirty" extraction methods may not be optimal for cDROP. This will be something that the protocol developing team will help you with.
"

"pros: Measure many samples simultaneously
We do not have to wipe out after every measurement as we do for NanoDrop
cons: Spend more consumables than the NanoDrop
Worn out after I perform many measurements because it is too hard to pick up the plate.
It is so useful when we perform a measurement for many samples, but it is unfortunate that we hardly measure so many samples.
"

Review date: 29 Feb 2016 | DropSense96® Content Analysis Platform

The DropSense™96 UV/VIS droplet reader is designed for high throughput quantification of native nucleic acid or protein samples. Combined with its unique microfluidic 96well DropPlates, micro-volume (2µl) quantification is made possible in a fully automated workflow. A complete software toolbox is available for detailed sample QC, robot integration and compliance in regulatory environments

The DropSense96® platform combines:

DropSense96 reader: the only high-throughput UV/VIS spectrophotometer for microliter sample volumes currently in the market, analyzing up to 96 samples in less than 5 minutes. The instrument can be used manually or integrated into any robot to ensure fully automated droplet quantification. A software toolbox is available for integration (API) and regulated labs (CFR 21 part11 ready).

Micro-volume DropPlates: microfluidic design for 2 µl samples loaded and sample preservation up to 2 hours. Sample analysis by micro-cuvettes generating an extensive measurement range from 0.1 up to 200 OD (10 mm conversion). The DropPlate96 is barcoded and has a SBS/ANSI footprint for compatibility with liquid robots.

Analytical software: DropQuant for classic UV/VIS analysis with A260 or A280 quantification. Add-on cDrop™ analytical package can be used for detailed content analysis of impure samples. The cDrop software uses the UV/VIS spectral data by the DropSense96® to extract specific and reliable quantitative information by defining the spectral contribution of the isolate and potential contaminants present. The software packages allow easy experimental design and LIMS compatible reporting.

The Trinean technology is the method of choice for QC and QA of biomolecules, given its sample purity analysis, dynamic measurement range, small sample consumption and operational efficiency.