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I assume that the dose of 2000 FTU/kg is too high. Similar results could be obtained by including 800-1000 FTU/kg in the feed. The study reflects only the facts of experience and the authors do not explain anything. Perhaps the explanation is in the full article. Reducing the calcium content in the diet, even without adding phytase, increases the absorption and use of phosphorus. For the development of research, it is interesting to determine the content of total and phytate phosphorus in feces. I suggest the formation of secondary phytates in the small intestine, which may reduce the availability of phosphorus. It is interesting to collect the contents of the jejunum (between jejunum and Ileum) and incubate in vitro with phytase Ronozyme ® HiPhos and Qwantum Blue. This will allow you to find out the effectiveness of phytase (preserving its activity) in the lower intestine. It is also interesting to incubate feces, which will help you find out whether the phytases completely break down the feed's phytates/

Valerij Kryukov, I understand that with your proposed evaluations, the objective to be achieved would be to prove the effective action of phytases in the hydrolysis of phytate, which was not the intention of this study. As for the dosage question, we used 2000 FTU / Kg based on a study available in the literature, where the authors found a linear effect of phytase concentration up to 2000 FTU / Kg in the feed on the performance of the piglets. Now the fact that the phytase concentrations of 800 - 1000 FTU / Kg would result in the same efficiency as the concentration of 2000 FTU / Kg was not proven in that work. I understand that this study would be restricted if the phytase concentration below was used of the 800 FTU / Kg, I believe that with the evaluations that were carried out, the objectives of the study were fully achieved. Thus, it can be deduced that the evaluated parameters were sufficient to validate the conclusions of this study. As for the questioning that the study explains nothing, it gives the understanding that a study in which it is proposed to evaluate levels of calcium and phosphorus in the diet with phytase supplementation, would only be valid if it used phytase in the concentration between 800 to 1000 FTU / Kg and that the evaluations were made in the intestinal digestion and in the feces. I reaffirm that these assessments would be essential if we were specifically evaluating the efficiency of action of phytase, which was not the case. .. I also understand that if you go into this detail, the phytase action of the intestinal mucosa of piglets should be evaluated. I also understand that the evaluations of blood and bone parameters performed reflect in some way the effectiveness of the action of phytase. I make these considerations in the sense that I am aware of my limitations and that I did not intend with this work to put an end to the researched subject.

Dear Juarez Donzele You can probably guess that you can come up with a lot of combinations of phytates, phytase and calcium, as well as the composition of compound feeds. There will be some differences in each experience. According to the work carried out, we can state a fact, but what is fundamentally new, except that You can refer to the fact that your results coincide with previously published ones. This will confirm that you know how to conduct experiments accurately. I always want something completely new. –let a little, but to tell: I made it and I will seat You. And You can agree or not-it drives science. Where will your article advance science? Please do not take offense at me, I sincerely expressed my opinion to you.I will add that discussing an article on a resume is not serious. Need the full textSincerely M. Kryukov

Dr Kryukov, I do not intend to dispute the issue, but in my understanding we have two main focuses in research, namely, basic and applied. I realize, from your considerations, that your vision is the advancement of science by science, generating basic information, without concern for the applied character of the research. . I respect your opinions, but I do not agree with all of them because I understand that conducting practical research is also doing science. I even think that applied research is the way we have to validate information obtained in the basics. As for your questioning that you always want something new that drives science, it confirms what I think about your research vision, and therefore, I think that you are not included in the target audience of this study. Finally, I say that I have no reason to be offended, quite the contrary, because I understand that in biology there is no owner of the truth. And that in science the contradiction is what makes it the development

How 2000 FTU vs 1000 depend upon 2 things: Total P of diet or level of PP. when limited, high dose phytase not to perform; The Phytase, not all one same, specially for pig. wide pH profile win longer time to digest PP.

Dear Prof. Donzele.Since Hiphos concentration is expressed in FYT, I believe that when you write FTU you mean FYT, right? Considering it has an equivalence around a 2:1 ratio to FTU, your level was really 1000 FTU/kg. Thank you.

Would you like to discuss about this topic: Effect of reduced dietary non-phytate phosphorus concentration, with or without calcium reduction, in diets supplemented with phytase for weaned piglets from 28 to 63 days of age?

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