Abstractα and β-Galactosidases producing Asperqillus terreus varieties MRU-26 and MLU-3 were isolated from local soil samples by enrichment culture technique. Enzymes were active and stable towards acid side and moderately heat stable. Either of the two enzymes exhibited specificity towards α-/β-Galactosidic bonds. Shake flasks study revealed that enzymes induction was non-specific. Mono, di, trisaccharides (irrespective of α-/β-linkage) induced α-/β-Galactosidases synthesis. Competitive inhibition of glucose in presence of inducers creates diauxic phenomenon in mycelial growth traces of enzyme formed in absence of inducers pointed towards there acutoctlylitic of nitrogen sources affected both enzyme formation and growth. Effect of organic nitrogen source was distinct from that of inorganic one . Combined effect of more than one nitrogen source gave better results. Effect of cultural techniques using agro-industrial by-products also affected α and β-Galactosidases synthesis. By semi-solid surface culture yield of both enzymes was almost constant. But in submerged culture (water soluble of the same raw material), variety of results were obtained with proportionately high values as compared to those by semi-solid culture. Bean meals were found to the most effective for galactosidase(s) synthesis than brans and detoxified seed meals