应用

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用

Ab评论

说明

IHC-FoFr

Use at an assay dependent concentration.

WB

Use at an assay dependent concentration.

Flow Cyt

1/10 - 1/20.

ab35768 - Rat monoclonal IgM, is suitable for use as an isotype control with this antibody.

ab35768-Rat monoclonal IgM, is suitable for use as an isotype control with this antibody.

ICC/IF

Use at an assay dependent concentration.

靶标

相关性

SSEA3 is a globoseries carbohydrate antigen present on both cell surface glycolipids and glycopeptides. It is found on the surface of mouse oocytes, becoming restricted to the intracellular mass of the early blastocyst and then to the primitive endoderm. It is present on the surface of human teratocarcinoma cells (EC), embryonic germ cells (EG) and embryonic stem cells (ES) but not mouse undifferentiated mouse cells of these types. SSEA3 expression decreases as human EC, ES and EG cells differentiate but increases on differentiation of mouse EC, ES and EG cells. It is also found on the surface of mouse erythrocytes.

Anti-SSEA3 antibody, ab16286 can be used as a marker of Embryonic Carcinoma cells in Flow Cytometry/FACS. The top histogram (A) shows undifferentiated NTERA2 cells (i.e. without retinoic acid) recognised by ab16286. However, upon differentiation (by the addition of retinoic acid 10-5M for 7 days), the antibody lost the ability to recognise these cells as shown in the bottom histogram (B).