VDR is a member of the superfamily of steroid hormone receptors. It is known to play a role in cellular growth and differentiation, calcium and phosphorus metabolism and bone remodeling. Human breast carcinomas, leukemias, and prostatic carcinoma cells express VDR, which can modify their growth and differentiation. In avians, VDT appears as a doublet of 58kD and 60kD.

Catalog #

V2130

Applications

Suitable for use in Western Blot. For Gel Supershift assays use V2130X. Other applications have not been tested.

Recommended Dilutions

Western Blot: 1-2ug/ml for 2 hours at RT

Optimal dilutions to be determined by the researcher.

Positive Control

LN cap cells and HeLa cells

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Clone Type

Monoclonal

Isotype

IgG2b

Clone No

0.N.603

Host

Rat

Source

Chicken

Concentration

~0.2mg/ml

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See V2130X.

Purity

Purified by Protein G affinity chromatography.

Immunogen

Vitamin D receptor protein from chicken intestine (53kD).

Cellular Localization

Nuclear. Epitope: aa89-105 (near DNA binding domain).

Specificity

Recognizes both the occupied and unoccupied forms of chicken VDR. Does not crossreact with estrogen or glucocorticoid receptors. In avians, VDR appears as a doublet of 58 and 60kD.

Species Crossreactivity

Porcine, mouse, rat, hamster, frog, human and fish.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.