Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

ICC/IF

Use at an assay dependent concentration.

ELISA

Use at an assay dependent concentration.

WB

Use at an assay dependent concentration.

Target

Relevance

The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.

Ab9115 and ab18184 are provided at 1mg/ml. For both antibodies I would recommend an initial dilution 1/1000 and try serial dilutions in order to optimise it to your experiment.

You can also have a look at the Abreviews from previous users as guidance, as well as our online protocols:

https://www.abcam.com/index.html?pageconfig=resource&rid=11375

Regarding the discounts, I am aware you can get 5% discount for purchases over 5 vials. In any case, for more accurate information about promotions, please contact our Customer Service Department: mailto:orders@abcam.com.

I hope this helps and if we can assist further, please do not hesitate to contact us.

Thank you very much for those details, it is very useful.
I am sorry you are not satisfied with ab9115.
In this instance, without admission of responsibility or liability, I am prepared to offer you a credit note or refund for the value of the antibody.
Please could you let me know which you would prefer and the Abcam order number or PO number so I can arrange it.
Many thanks for your patience,