APEX® Antibody Labeling Kits utilize a unique solid-phase labeling method that enables an efficient, convenient and reliable method for covalently attaching fluorescent labels to very small amounts of IgG antibody in the presence of any contaminants including stabilizing proteins and amine-containing buffers.

Convenience—Requires only 10–20 µg of IgG antibody per labeling reaction, regardless of any purity limitations

Complete—Includes everything required to perform 5 separate labelings

How APEX® Antibody Labeling Kits work

The APEX® Antibody Labeling Kits use a solid-phase labeling technique that captures the IgG antibody on the resin inside the APEX® antibody labeling tip (Figure 1). Any contaminants, including stabilizing proteins or amine-containing buffers, are simply eluted through the tip. After applying the amine-reactive label, a fluorescent IgG conjugate is ready for use in any imaging or flow cytometry application in as little as 2.5 hours with very little hands-on time. The typical yield of labeled antibody using this method ranges from 40–80%.

The APEX® Antibody Labeling Kits includes all reagents required to perform 5 separate labeling reactions of 10–20 μg of IgG antibody with one of Molecular Probes® superior fluorophores or biotin-XX. The biotin-XX provides a 14-atom spacer between the antibody and the biotin moiety.