In a BY4735 background, knocking out YPL068C seems to have a moderate effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 124 minutes, while the YPL068C knock-out strain's doubling time was 209 minutes.

In the BY4735 background, knocking out YPL068C seems to have a little effect on the growth rate. In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, whereas the YPL068C knock-out strain's doubling time was 235 minutes. The calibration experiment, the BY4735 strain's doubling time was 64 minutes, whereas the YPL068C knock-out strain's doubling time was 92 minutes.

Methanol Sensitivity

Stressing with Hydroxyurea

Hydroxyurea Protocol
-The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YPL068C showed about a 30 minute difference in doubling time compared to the wild type strain, indicating that this gene most likely plays some role in DNA replication.

Fermentation

Fermentation protocol link [2]
- There appears to be no major difference in fermentation rate. The lower final ethanol percentage could be a result of evaporation. If this experiment were to be carried out again then it should be done in an air tight container.

Caffeine Sensitivity

Caffeine PRotocol [[UW-Stout/Caffeine], the genes reacted the same at the beginning, varied in the middle then came back together at the end.

Stressing with pH

When I looked at the data this graph shows I see that both the Wild type and also the yeast strain YPL068C have about the same linear pathway and so I came to the conclusion that even though the yeast strain YPL068C has somewhat a deviation difference but its still small enough and somewhat close enough to the wild types and so therefore both of them are yet resilient to the pH 4 stress test.

Cold Shock Sensitivity

The Results from this graph have proven that compared to the wild type there was no significant change in the doubling time. This has proven that its neither more or less sensitive in comparison to the wild type.