MinElute Reaction Cleanup Kit

For cleanup of up to 5 μg DNA (70 bp to 4 kb) from enzymatic reactions

Very small elution volumes

Fast procedure and easy handling

High, reproducible recoveries

Gel loading dye for convenient sample analysis

The MinElute Reaction Cleanup Kit provides spin columns, buffers, and collection tubes for silica membrane-based purification of DNA 70 bp – 4 kb in size from enzymatic reactions. The spin columns are designed to allow elution in very small volumes (as little as 10 μl), delivering highly concentrated DNA in high yields. An integrated pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on the QIAcube. DNA fragments purified with the MinElute system are ready for direct use in all applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription.
For optimal results it is recommended to use this product together with QIAvac 24 Plus.

The MinElute Reaction Cleanup Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Performance

The MinElute Reaction Cleanup Kit ensures cleanup of up to 5 μg DNA (70 bp to 4 kb) from enzymatic reactions, delivering high yields of DNA suitable for a range of applications. The kit provides spin columns for cleanup of enzymatic reactions. Using a microcentrifuge or vacuum manifold, high concentration of DNA fragment (70 bp – 4 kb) is quickly achieved. (DNA fragments larger than 4 kb should be purified using the QIAquick System.)

Examples of enzymes that are completely removed by the MinElute Reaction Cleanup Kit

The MinElute system uses a simple bind-wash-elute procedure (see flowchart "MinElute procedure"). Binding buffer is added directly to the enzymatic reaction, and the mixture is applied to the MinElute spin column. The binding buffer contains a pH indicator, allowing easy determination of the optimal pH for DNA binding (see figure "pH Indicator Dye"). Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Handling

MinElute spin columns are designed to provide two convenient handling options (see flowchart "MinElute procedure"). The spin columns fit into a conventional table-top microcentrifuge or onto any vacuum manifold with luer connectors, such as QIAvac 24 Plus or QIAvac 6S with QIAvac Luer Adapters. The MinElute Reaction Cleanup Kit, in addition to other QIAGEN spin-column-based kits, can be fully automated on the QIAcube, enabling increased productivity and standardization of results (see figures "Spin column handling options A, B, C, D, and E").

Applications

DNA fragments purified with the MinElute System are ready for direct use in all applications, including: