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Application Note: Considerations for Automating the Purification of Monoclonal Antibodies

5 Nov 2012

Monoclonal antibody (mAb) use in biomedical research allows specific molecule binding to occur, aiding in subsequent identification and isolation for multiple disease treatments. For a single‐step, small‐scale purification with high purity results, monoclonal antibodies are purified using affinity columns. Conventional manual spin techniques or liquid chromatography systems are typical purification approaches, but it is also possible to purify antibodies with a simple liquid handler and syringe pump. This application note from Gilson, discusses the advantages of moving from manual to automated mAb purification and the hardware and software considerations.