@article {,
title = {Protective function of the recombinant Oshsp18.0-II protein, a class II small heat shock protein of rice, in Escherichia coli. },
number = {10001},
year = {2012},
month = {2012 / 7},
pages = {8},
edition = {3},
chapter = {291},
abstract = {All plants synthesize multiple families of small heat shock proteins (sHSP). A cDNA clone, Oshsp18.0-CII, encoding an 18.0 kDa class II sHSP was isolated from rice previously. The function of the rice class II sHSP was studied by overproduction of the Oshsp18.0-CII fusion protein in transformed Escherichia coli cells. The results suggest that heterologous expression of the Oshsp18.0-CII fusion protein increases thermotolerance of E. coli cells in vivo and provided thermoprotection to E. coli soluble proteins in vitro. The survival rate of Oshsp 18.0-CII fusion protein accumulating cells treated at 50{\textdegree}C for 1 h was almost 1000-fold higher than that of the control cells transformed with pET32a expression vector. Overproduction of the Oshsp18.0-CII fusion protein in E. coli also confers tolerance of E. coli cells to ultraviolet (UV) irradiation. The post-UV survival of the Oshsp18.0-CII fusion protein-accumulating cells is about 7.29-fold over that of the control cells transformed with pET32a expression vector when exposed to 1700 μJ of UV. There is almost no post-UV survival (≦0.4\%) in the untransformed cells after exposing to 900 μJ UV light.},
author = {張碧芳 and 黃文寬 and 林盈宏 and 陳裕星 and 張道禾}
}