The traditional owners of the Galilee Basin region in central Queensland are appealing to the United Nations to stop the Adani Carmichael coal mining project.

The Wangan and Jagalingou Family Council said it was requesting urgent intervention under the UN's Declaration of the Rights of Indigenous Peoples.

Spokesman Adrian Burragubba said the traditional owners voted down a land use agreement with Adani almost 12 months ago, but their objections had been overlooked by both the State and Federal governments.

"We haven't consented to them destroying our traditional homelands, and we don't take any of their offers," he said.

Mr Burragubba said a separate action before the Federal Court was challenging a native title process that was overriding the rights of traditional owners.

He said the coal mine would permanently destroy sacred sites, including Doongmabulla Springs.

"Water is drawn from the Great Artesian Basin into the Carmichael River and into the Belyando River.

"We have stories about the Rainbow Serpent and our totems that live within that river.

"These are our stories and this is our Dreaming. Without these stories we can't go forward as a people. We'll be annihilated. There will be nothing left for us to identify with."

Approval for the Adani project was set aside by the Federal Court in August on environmental grounds.