Application Information: Vimentin antibody [VI-RE/1]

ELISA: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. WB: Use at an assay dependant dilution. Detects a band of approximately 57 kDa. This antibody recognizes a different epitope on human vimentin than GTX27752, making them suitable as an ELISA pair. Optimal dilutions/concentrations should be determined by the end user.

Form Supplied

Liquid

Concentration

1
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

>95% by SDS-PAGE

Purification Note

Purified from hybridoma culture supernatant by protein A affinity chromatography.

Specifications: Vimentin antibody [VI-RE/1]

Full Name

vimentin

Background

Vimentin is the major subunit protein of the intermediate filaments of mesenchumal cells, and has a molecular weight of 57 kD. Immunohistochemical staining for Vimentin is thus characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas, which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of malignant tumors, generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

Synonyms

FLJ36605, P08670, 193060, Vimentin, 7431, VIM

Cellular Localization

Cytoplasmic

Host

Mouse

Clonality

Monoclonal

Clone Name

VI-RE/1

Isotype

IgG1

Immunogen

Recombinant full length protein (Human).

Antigen Species

Human

Species Reactivity

Human

Conjugation

Unconjugated

Storage Conditions: Vimentin antibody [VI-RE/1]

Storage Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage Instruction

Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Notes

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.