Purpose: To use single-cell RNA-Seq analysis of nephron progenitors in order to determine transcrptional differences as nephron progenitors age. Methods: Using a combination of FACS sorting and a Fluidigm Single-cell auto-prep system, we generated high-throughput RNA-SEQ data of nephron progenitors during development Results: Single cells transcriptome profiling of nephron progenitors revealed progressive age-dependent changes with heterogeneity increasing in older populations. Overall design: 96-single cell transcriptomes were determined from nephron progenitors of e14.5, e18.5 and P0 using Cited1GFP transgenic animals

SRA archive data

SRA archive data is normalized by the SRA load process and used by the SRA Toolkit to read and produce formats like FASTQ, SAM, etc.
The default toolkit configuration enables it to find and retrieve SRA runs by accession.

Public SRA files are now available from GCP and AWS cloud platforms as well as from NCBI.
Access to most data in the cloud requires a user account with the cloud service provider.
The user’s account will incur costs for cloud compute or to copy data outside of the specified cloud service region.

In order to support large scale (hyper parallel) data analyses SRA data is now available at GCP and AWS with few caveats:

SRA data is copied to the cloud from NCBI. There may be a lag between availability from NCBI and from CSP (cloud service providers)

To access public data user account with the cloud service provider is required. Your account will incur costs for cloud compute and/or to copy data
(either archival or results of your comute) outside of the specified cloud service region

Distribution of protected data is signed by NIH account and requires user to operate in the same region as the data

SRA has also begun to provide access to originally submitted source files:

not all files have been validated by SRA

not all files have been copied to cloud locations (recovering it from NCBI tape system takes time ).

the volume of this type of data a much larger and it is not used as often so we will keep most of it
on tape or "cold" storage in cloud. As a result the data may not be available instantly and restore
requests will be served on first-come first-served basis and cost of resore may be charged to your
user account.

Select a chromosome and nucleotide range then
view as Sequence Viewer track or
download the reads aligned to this region. The range can be set
as 'from-to' or 'from..length'.
Warning: Local references cannot be displayed by Sequence Viewer.