Description: Lens culinaris lectin (LCH) is isolated from lentil seeds by affinity chromatography. This lectin has specificity for terminal a-D-Mannosyl and a-D-Glucosyl residues. Lens culinaris lectin has been found to stimulate human lymphocytes in culture to incorporate 3H-Thymidine. Lens culinaris lectin is comprised of two isomers: Lens culinaris hemagglutinin A (LCH-A) and Lens culinaris hemagglutinin B (LCH-B). The two LCH's differ in amino acid content and electrophoretic mobility.

Reconstitution: Reconstitute with Buffer to a concentration of 1mg/ml. Store liquid frozen in aliquots. Avoid freeze-thaw cycles. Clarify by centrifugation. After reconstitution the material is stable for a least 1 year when stored frozen in aliquots with 0.1% sodium azide added as a preservative.

Carbohydrate Specificity:a-Mannose>a-Glucose, N-Acetylglucosamine

Inhibitory Carbohydrate: D -Mannose and D-Glucose. A fucose linked a(1,6) to the core GlcNAc of N-linked glycopeptides is an important determinant for lectin activity.