岡山医学会Acta Medica Okayama0030-155812832016集学的治療により長期生存が得られた食道神経内分泌癌の2 例207212ENTatsuhiroGotodaDepartment of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical SciencesSeijiKawanoDepartment of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical SciencesYoshiyasuKonoDepartment of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical SciencesKouMiuraDepartment of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical SciencesHiromitsuKanzakiDepartment of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical SciencesMasayaIwamuroDepartment of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical SciencesYoshiroKawaharaDepartment of Endoscopy, Okayama University HospitalTakehiroTanakaDepartment of Pathology, , Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical SciencesTadashiYoshinoDepartment of Pathology, , Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical SciencesYasuhiroShirakawadDepartment of Gastroenterological Surgery, Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical SciencesMasahiroTabataDepartment of Hematology and Oncology, Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical SciencesMitsuneTanimotoDepartment of Hematology and Oncology, Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical SciencesHiroyukiOkadaDepartment of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine， Dentistry and Pharmaceutical Sciences Esophageal neuroendocrine carcinoma （ECC） is rare and has a poor prognosis when presenting with vascular invasion and distant metastasis from an early stage. Multidisciplinary therapy with surgery, chemotherapy, and radiation therapy may prolong survival in patients with advanced ECC, but there is as yet no standard therapy for advanced ECC. We treated two patients who have achieved long-term survival （＞ 4 years） who underwent multidisciplinary therapy, including chemotherapy, for ECC. Our experience with these two cases suggests that multidisciplinary therapy, including chemotherapy, may be effective for treating ECC at an advanced stage.No potential conflict of interest relevant to this article was reported.
食道神経内分泌腫瘍（esophageal neuroendocrine carcinoma）
小細胞癌（small cell carcinoma）
集学的治療（multidisciplinary therapy）
岡山医学会Acta Medica Okayama0030-155812822016拡大内視鏡検査にて形態変化を観察しえた十二指腸濾胞性リンパ腫の1 例111116ENMasayaIwamuroDepartment of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesKatsuyoshiTakataDepartment of Pathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesSeijiKawanoDepartment of Endoscopy, Okayama University HospitalYoshiroKawaharaDepartment of Endoscopy, Okayama University HospitalTadashiYoshinoDepartment of Pathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesHiroyukiOkadaDepartment of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences A 63-year-old Japanese woman was diagnosed with duodenal follicular lymphoma. The initial esophagogastroduodenoscopic examination with magnifying observation revealed opaque white spots and enlarged whitish villi. Nine months later, esophagogastroduodenoscopy showed that the size of the lymphoma lesion decreased, and only opaque white spots were visible. The histological analysis of biopsy samples obtained during the initial endoscopy examination showed both neoplastic follicles and an inter-follicular infiltration of lymphoma cells, whereas the biopsy samples obtained at the endoscopy performed 9 months later showed only neoplastic follicle formation. These results suggest that the magnifying endoscopic features may reflect the underlying pathological mechanisms : enlarged whitish villi are probably due to lymphoma cell infiltration in the inter-follicular area, and opaque white spots are probably caused by neoplastic follicle formation.No potential conflict of interest relevant to this article was reported.
消化管原発濾胞性リンパ腫（primary gastrointestinal follicular lymphoma）
悪性リンパ腫（malignant lymphoma）
拡大内視鏡検査（magnifying endoscopy）
Okayama University Medical SchoolActa Medica Okayama0386-300X6912015Magnified Endoscopic Features of Duodenal Follicular Lymphoma and Other Whitish Lesions3744ENMasayaIwamuroHiroyukiOkadaKatsuyoshiTakataYoshinariKawaiSeijiKawanoJunichiroNasuYoshiroKawaharaTakehiroTanakaTadashiYoshinoKazuhideYamamotoOriginal Article10.18926/AMO/53120The sensitivity and specificity of magnified endoscopic features for differentiating follicular lymphoma from other diseases with duodenal whitish lesions have never been investigated. Here we compared the magnified endoscopic features of duodenal follicular lymphoma with those of other whitish lesions. We retrospectively reviewed the cases of patients with follicular lymphoma (n＝9), lymphangiectasia (n＝7), adenoma (n＝10), duodenitis (n＝4), erosion (n＝1), lymphangioma (n＝1), and hyperplastic polyp (n＝1). The magnified features of the nine follicular lymphomas included enlarged villi (n＝8), dilated microvessels (n＝5), and opaque white spots of various sizes (n＝9). The lymphangiectasias showed enlarged villi, dilated microvessels, and white spots, but the sizes of the white spots were relatively homogeneous and their margin was clear. Observation of the adenoma and duodenitis revealed only whitish villi. Although the lymphangioma was indistinguishable from the follicular lymphomas by magnified features, it was easily diagnosed based on the macroscopic morphology. In conclusion, magnified endoscopic features, in combination with macroscopic features, are useful for differentiating follicular lymphomas from other duodenal diseases presenting whitish lesions.No potential conflict of interest relevant to this article was reported.
duodenal neoplasm
follicular lymphoma
gastrointestinal lymphoma
magnifying endoscopy
Acta Medica Okayama0046-81774572014Low-grade B-cell lymphoma presenting primarily in the bone marrow13791387ENKayokoIwataniKatsuyoshiTakataYasuharuSatoTomokoMiyata-TakataNorikoIwakiWeiCuiSeikoSawada-KitamuraHiroshiSonobeMaikoTamuraKatsuhikoSaitoKatsuyaMiyataniRieYamasakiIchiroYamadoriNobuharuFujiiYasushiTerasakiYoshinobuMaedaMitsuneTanimotoNaoyaNakamuraTadashiYoshinoCases of low-grade B-cell lymphoma presenting primarily in the bone marrow are rare, and its clinicopathology remains unclear. We retrospectively examined patients with low-grade B-cell lymphoma presenting primarily in the bone marrow. Fourteen patients met the inclusion criteria, including 5 with lymphoplasmacytic lymphoma (LPL), 3 with chronic lymphocytic leukemia/small lymphocytic lymphoma, 2 with follicular lymphoma (FL), and 4 with low-grade B-cell lymphoma not otherwise specified (LGBCL-NOS). The median age was 69.5 years (range, 42-89 years), and a slight male predominance was noted (9 men and 5 women, 1.8: 1). Immunohistochemically, all cases were positive for CD20. One case was positive for CD138. Both cases of FL were positive for CD10 and B-cell lymphoma 2 (BCL-2), and immunoglobulin heavy locus (IgH)/B-cell lymphoma 2 rearrangement was observed by fluorescence in situ hybridization. The myeloid differentiation primary response gene (88) leucine to proline mutation was observed in 3 of 5 LPL, 1 of 2 FL, and 2 of 4 LGBCL-NOS patients. Paraproteinemia was observed in 10 patients; IgM and IgG paraproteinemia were observed in 6 and 3 patients, respectively. In this patient series, 3 patients had died at a median follow-up of 36.5 months; the cause of death of 1 LPL patient was malignant lymphoma itself. Thus, low-grade B-cell lymphoma presenting primarily in the bone marrow has various subtypes, and approximately one-third of the patients had LGBCL-NOS. The immunophenotypic features and myeloid differentiation primary response gene (88) leucine to proline mutation data of LGBCL-NOS suggested that some cases present with characteristics similar to those of LPL or marginal zone lymphoma.No potential conflict of interest relevant to this article was reported.
Low-grade B cell lymphoma
Bone marrow
LGBCL-NOS
MYD88
Acta Medica Okayama1618-124710022012Histological and immunohistochemical features of gingival enlargement in a patient with AML254257ENNorihiroSonoiYoshihikoSogaHiroshiMaedaKoichiIchimuraTadashiYoshinoKazutoshiAoyamaNobuharuFujiiYoshinobuMaedaMitsuneTanimotoRichardLoganJudithRaber-DurlacherShogoTakashibaHere, we discuss the pathophysiology of leukemia-associated gingival enlargement based on a case of acute myelomonocytic leukemia (AML-M4) with typical gingival enlargement. Uniquely, this patient was well enough to allow full periodontal examination and incisional gingival biopsy to be performed both before and after chemotherapy. The patient was a 39-year-old Japanese woman with AML-M4 showing gingival enlargement. Histological and immunohistochemical features of gingiva and bacterial counts in the periodontal pockets were examined before and after chemotherapy. The results were as follows: (1) infiltration of myelomonocytic blasts in enlarged gingiva; (2) resolution of gingival enlargement with complete remission of AML by anticancer chemotherapy; and (3) the numbers of bacteria in the periodontal pockets were not high and were not altered before or after chemotherapy. In patients with AML-M4, remarkable mucosal enlargement is not generally observed in the body except in the gingiva. We hypothesized that antigens derived from periodontal bacteria, even if they are not present in large numbers, could act as chemoattractants for myelomonocytic leukemic cells.No potential conflict of interest relevant to this article was reported.
Gingival enlargement
Acute myelomonocytic leukemia
Pathogenesis
Histology
Immunohistochemistry
Public Library ScienceActa Medica Okayama1932-6203822013A20 (TNFAIP3) Deletion in Epstein-Barr Virus-Associated Lymphoproliferative Disorders/LymphomasENMidoriAndoYasuharuSatoKatsuyoshiTakataJunkoNomotoShigeoNakamuraKoichiOhshimaTamotsuTakeuchiYorihisaOritaYukioKobayashiTadashiYoshinoA negative regulator of the nuclear factor (NF)-kappa B pathway, A20 (TNFAIP3), is inactivated in several types of lymphomas; particularly in diffuse large B-cell lymphoma (DLBCL), classical Hodgkin's lymphoma, and extranodal marginal zone lymphoma of the mucosa-associated lymphoid tissue. These findings suggest that the NF-kappa B activation is related to A20 inactivation. Recently, A20 inactivation has also been observed in Epstein-Barr virus (EBV)-related lymphomas; however, this occurrence has not been well investigated. Moreover, NF-kappa B is a key molecule in activated B-cell-like (ABC)-type DLBCL; EBV-associated DLBCL is of the ABC type. Therefore, we focused on A20 deletions in EBV-associated lymphoproliferative disorders/lymphomas. Using fluorescent in situ hybridization analysis, A20 deletions were identified in 4 of 13 samples from patients with pyothorax-associated lymphoma (PAL) (31%), 3 of 20 samples from nasal-type NK/T cell lymphomas (NKTLs) (15%), 1 of 8 samples of EBV-positive DLBCL of the elderly (DLBCL-e) (13%), but not in any of the 11 samples from individuals with methotrexate-related lymphoproliferative disorder (MTX-LPD) (0%). Among the samples with A20 deletions, EBV latent membrane protein 1 (LMP-1) expression was detected in all 4 of the PAL samples with A20 deletions and in the DLBCL-e sample with an A20 deletion, but not in any of the 3 NKTL samples. This finding indicated that A20 deletions were not directly related to the EBV latency pattern of lymphomas, although such deletions might be related to the diagnostic category. Immunohistologically, the A20 protein was absent in 2 (15%) of the13 PAL samples, 1 (9%) of 11 MTX-LPD samples, and in none of the 20 NKTL (0%) or 8 DLBCL-e samples. In conclusion, A20 deletion and/or dysfunctional expression are frequently associated with PALs, and A20 abnormalities may be related to the pathogenesis of PAL.No potential conflict of interest relevant to this article was reported.Acta Medica Okayama0945-631746352013In aggressive variants of non-Hodgkin lymphomas, Ezh2 is strongly expressed and polycomb repressive complex PRC1.4 dominates over PRC1.2697711ENLamiaAbd Al KaderTakashiOkaKatsuyoshiTakataXuSunHiakiSatoIchiroMurakamiTomohiroTojiAkihiroManabeHiroshiKimuraTadashiYoshinoPolycomb group (PcG) proteins are important for the regulation of hematopoiesis by regulating chromatin compaction and silencing genes related to differentiation and cell cycle. Overexpression of enhancer of zeste homologue 2 (Ezh2) and Bmi-1/PCGF4 has been implicated in solid organ cancers, while Mel-18/PCGF2 has been reported as a tumor suppressor. Detailed expression profiles of PcG proteins and their diagnostic significance in malignant lymphomas are still unknown. In this study, we analyzed the expression levels of Ezh2, Bmi-1, Mel-18, and Ki67 in 197 Hodgkin's and non-Hodgkin's lymphoma patient samples and in lymphoma cell lines using immunohistochemistry, fluorescent immunocytochemistry, and Western blotting. Immunohistochemical staining showed that Ezh2 expression was significantly increased in aggressive compared to indolent subtypes of B cell neoplasms (P = 0.000-0.030), while no significant differences in Bmi-1 expression were found between these subtypes. Compared to the normal counterpart, T cell lymphomas showed significant overexpression of Bmi-1 (P = 0.011) and Ezh2 (P = 0.000). The Ki67 labeling index showed a positive correlation with Ezh2 expression in B cell lymphomas (correlation coefficient (Co) = 0.983, P = 0.000) and T/NK cell lymphomas (Co = 0.629, P = 0.000). Fluorescent immunohistochemical staining showed coexpression of Ezh2 and Ki67 in the same tumor cells, indicating that Ezh2 expression correlates with cell proliferation. Both B and T/NK cell neoplasms showed low expression of Mel-18 and high expression of both Bmi-1 and Ezh2. In conclusion, in aggressive lymphoma variants, Ezh2 is strongly expressed and polycomb repressive complex PRC1.4 dominates over PRC1.2. Coexpression of Bmi-1 and Ezh2 is a characteristic of aggressive lymphomas. Ezh2 correlates with the proliferation and aggressive nature of non-Hodgkin's lymphomas.No potential conflict of interest relevant to this article was reported.
Ezh2
Bmi-1
Mel-18
Malignant lymphoma
PRC1.2
PRC1.4
Acta Medica Okayama0046-81774492013Distinct morphologic, phenotypic, and clinical-course characteristics of indolent peripheral T-cell lymphoma19271936ENEikoHayashiKatsuyoshiTakataYasuharuSatoYukieTashiroYoshiroTachiyamaSeikoSawada-KitamuraYasushiHiramatsuShunSugiguchiSoichiroNoseMitsuyoshiHirokawaMidoriAndoLamiaAbd MaderYoshinobuMaedaMitsuneTanimotoTadashiYoshinoPeripheral T-cell lymphoma, not otherwise specified (PTCL-NOS) consists of a heterogeneous group of lymphomas. Patients. generally show an aggressive clinical course and very poor outcome. Although the 2008 World Health Organization classification of PTCL-NOS includes 3 variants, low-grade lymphoma is not Included. Of 277 PTCL-NOS cases recorded in our consultation files, we examined the clinicopathologic characteristics of 10 patients with T-cell lymphomas composed of small-sized cells with slight nuclear atypia. Eight patients showed extranodal involvement (5 patients, spleen; 3 patients, thyroid), and 5 patients were at clinical stage I or II. Histologically, all samples presented diffuse infiltrate of small lymphoid cells, with few mitotic figures. Immunohistologically, all samples were positive for CD3, and CD:20 Was detected in 5 samples. All samples showed a low Ki-67 labeling index (mean, 1.05%), and 7 samples were positive for central memory T-cell markers. Clonal T-cell receptor gamma chain and/or alpha-beta chain gene rearrangements were detected in all 10 patients. Five patients received chemotherapy, whereas for 3 patients, treatment consisted only of observation following surgical resection of the spleen or thyroid. Nine patients were alive at a median follow-up time of 19.5 months, whereas 1 patient died of an unrelated disease. The present study strongly indicates that T-cell lymphoma with small-sized lymphoma cells and a low Ki-67 labeling index is a distinct variant. Recognition of this novel lymphoma subtype, which should not be defined merely as PTCL-NOS, should be seriously considered.No potential conflict of interest relevant to this article was reported.
Indolent PTCL
CD20
Ki-67
Memory T cell
Good prognosis
Public Library ScienceActa Medica Okayama1932-6203822013Clinicopathologic Analysis of Localized Nasal/Paranasal Diffuse Large B-Cell LymphomaENHirokoTodaYasuharuSatoKatsuyoshiTakataYorihisaOritaNaokoAsanoTadashiYoshinoDiffuse large B-cell lymphoma (DLBCL) comprises 2 molecularly distinct subgroups of non-germinal center B-cell-like (non-GCB) and germinal center B-cell-like (GCB) DLBCLs, with the former showing relatively poor prognosis. In the present study, we analyzed the clinicopathological features of 39 patients with localized nasal/paranasal DLBCL. Immunohistochemistry-based subclassification revealed that 11 patients (28%) were of the GCB-type according to Hans' algorithm and 11 (28%) were of the GCB-type according to Choi's algorithm. According to both Hans' and Choi's algorithms, the non-GCB type was predominant. Nevertheless, prognosis was good. Overall survival did not differ significantly between the GCB and non-GCB subgroups (Hans' algorithm: p = 0.57, Choi's algorithm: p = 0.99). Furthermore, the prognosis of localized nasal/paranasal DLBCL was better than that of other localized extranodal DLBCLs. The prognosis of extranodal DLBCL is usually considered poorer than that of nodal DLBCL. However, in our study, no difference was noted between patients with localized nasal/paranasal DLBCL and patients with localized nodal DLBCL. In conclusion, although the non-GCB subtype is thought to show poor prognosis, in our study, the prognosis for localized nasal/paranasal DLBCL patients was good irrespective of subclassification.No potential conflict of interest relevant to this article was reported.Okayama University Medical SchoolActa Medica Okayama0386-300X6742013Large Ulceration of the Oropharynx Induced by Methotrexate-Associated Lymphoproliferative Disorders265269ENHiroyukiHanakawaYorihisaOritaYasuharuSatoKinyaUnoKazunoriNishizakiTadashiYoshinoCase Report10.18926/AMO/51072We present a case of a 67-year-old Japanese man with a serious oropharyngeal ulceration that at first seemed to be destructive malignant lymphoma or oropharyngeal carcinoma. We suspected methotrexate (MTX)-associated lymphoproliferative disorder (LPD) induced by MTX treatment for rheumatoid arthritis (RA). About 3 weeks after simple discontinuation of MTX, complete regression of the disease was observed, confirming our diagnosis.No potential conflict of interest relevant to this article was reported.
ulceration
methotrexate
oropharynx
lymphoproliferative disorders
Wiley-BlackwellActa Medica Okayama0902-44419022013Morphologic, flow cytometric, functional, and molecular analyses of S100B positive lymphocytes, unique cytotoxic lymphocytes containing S100B protein99110ENYukariMikiYukaGionYurikoMukaeAtsushiHayashiHiakiSatoTadashiYoshinoKiyoshiTakahashiLittle is known about the S100B+ lymphocytes, which are unique human peripheral blood lymphocytes (PBL) containing the S100B protein. It has recently been shown that S100B is released from various types of S100B+ cells and exhibits varied cytokine-like activities. In this study, we precisely characterized the S100B+ lymphocytes of healthy adults with respect to the proportion in the whole PBL, immunophenotypes, function, and their S100B mRNA expression and also evaluated their S100B-releasing activity upon stimulation. S100B+ lymphocytes were detected in all individuals examined, and the proportion of S100B+ lymphocytes in the whole PBL ranged from 0.42% to 16.15% (mean, 4.21%). In addition, two subtypes of S100B+ lymphocytes, a CTL subtype (CD3+ CD8+ CD16-) and a NK subtype (CD3- CD8- CD16+), were detected. The majority of the CTL subtype of S100B+ lymphocytes expressed the alpha beta-T-cell receptor. Surprisingly, S100B mRNA was detected not only in S100B+ lymphocytes, but also in every S100B- lymphocytes, although the expression levels of S100B mRNA in S100B- lymphocytes were much lower than those of S100B+ lymphocytes. The CTL subtype of S100B+ lymphocytes exhibited blastic morphological changes, proliferated and released S100B upon stimulation with phytohemagglutinin. The NK subtype of S100B+ lymphocytes exhibited morphological NK activity when cocultivated with NK-sensitive target, K-562 cells. Thus, the CTL subtype of S100B+ lymphocytes exhibit the biological characteristics of T cells, while the NK subtype of S100B+ lymphocytes exhibit the characteristics of NK cells. These results suggest that S100B+ lymphocytes are a particular subtype of cytotoxic lymphocytes that play a unique role in antitumor immunity.No potential conflict of interest relevant to this article was reported.
S100B
peripheral blood
cytotoxic T cells
NK cells
岡山医学会Acta Medica Okayama0030-155812522013十二指腸濾胞性リンパ腫はAIDの発現を欠くがBACH2の発現を有しmemoryB細胞としての性質を有する103107ENKatsuyoshiTakataYasuharuSatoNaoyaNakamuraMamiTokunakaYukariMikiYara YukieKikutiKazuhikoIgarashiEtsuroItoHideoHarigaeSeiichiKatoEikoHayashiTakashiOkaYoshinobuHoshiiAkiraTariHiroyukiOkadaABD Alkader LamiaMohamadoYoshinobuMaedaMitsuneTanimotoTomohiroKinoshitaTadashiYoshinoNo potential conflict of interest relevant to this article was reported.
follicular lymphoma
gastrointestinal tract
BACH2
memory B cell
岡山医学会Acta Medica Okayama0030-155812522013外傷性脳傷害に対する抗HMGB-1抗体治療97102ENYuOkumaKeyueLiuHidenoriWakeJunHarumaTadashiYoshinoAijiOhtsukaHideoTakahashiShujiMoriMasahiroNishiboriIsaoDateNo potential conflict of interest relevant to this article was reported.
HMGB-1
traumatic brain injury （頭部外傷）
secondary injury （二次的損傷）
blood brain barrier （血液脳関門）
Okayama University Medical SchoolActa Medica Okayama0386-300X4661992Detection of oncogene rearrangements in human non-Hodgkin's lymphomas.407415ENEisakuKondoTadashiYoshinoTadaatsuAkagiKazuhikoHayashiKiyoshiTakahashiArticle10.18926/AMO/32636<p>Southern blot hybridization was used to detect the rearrangement and amplification of five proto-oncogenes (bcl-2, bcl-1, c-myc, c-myb and c-Ha-ras) and one tumor suppressor gene (RB-1) in 55 Japanese patients with non-Hodgkin's lymphoma; 16 with T-cell lymphomas and 39 with B-cell lymphomas (7 follicular and 32 diffuse lymphomas). Genetic abnormalities of the proto-oncogenes were detected in 7 of the 55 (13%). Genetic abnormalities of bcl-2 plus other genes were detected in 5 of 7 cases of follicular lymphoma (71%), rearrangements of bcl-2 and c-myc, rearrangement of bcl-2 and amplification of c-myb. Genetic abnormalities were observed in only three cases of diffuse lymphoma. In each of 3 cases of B-cell lymphoma, one of the genes, blc-2 mbr, bcl-2 mcr and c-myc, was rearranged respectively. The incidence of genetic abnormalities in diffuse lymphomas (6.3%) was lower than that in follicular lymphomas. None of diffuse lymphomas had double oncogene abnormality. No abnormalities were found in RB-1, bcl-1, and Ha-ras. These findings suggest that follicular lymphomas are associated with some abnormalities of oncogenes not restricted to bcl-2 that facilitate growth which may be associated with their clinical features.</p>No potential conflict of interest relevant to this article was reported.
malignant lymphoma
cellular oncogenes
Okayama University Medical SchoolActa Medica Okayama0386-300X4641992Establishment and characterization of a cell line, KaMi, from human lung large cell carcinoma.257264ENHiroshiTakataTadashiYoshinoYoshihikoHoshidaIkukoTakataTadaatsuAkagiArticle10.18926/AMO/32620<p>A cell line of human lung large cell carcinoma (LCC) was established directly from the metastatic skin tumor tissue. The clinical course of the patient who carried this carcinoma was peculiar; generalized lymphadenopathy, histologically resembling Hodgkin's disease, was found as the first clinical symptom. The lung tumor was not discovered until the time of autopsy. This cell line (KaMi) grew adherent to culture vessels with the population doubling time of 20.6h, formed colonies in soft agars with efficiency of 22.6%, and formed tumors in athymic nude mice. The authenticity of KaMi was confirmed by chromosomal analysis and isoenzyme patterns. KaMi cells bore a strong resemblance to the original tumor cells which were composed of small spindle cells, large polygonal cells, and multinucleated giant cells. Immunohistochemically, KaMi cells showed a weak tendency to differentiate to squamous cells, and these immunohistochemical reactivities were almost compatible to those of the original tumor cells, but ultrastructurally, KaMi cells were more immature than the original ones. Treatment with several reagents could not augment a differentiation of KaMi cells. Cytokeratin profiles showed a tendency of squamous cell differentiation. KaMi cells may aid in elucidating the pathogenesis and biology of LCC and its relationship to other lung tumors.
</p>
No potential conflict of interest relevant to this article was reported.
Large cell lung carcinoma
cell line
cytokeratin
Okayama University Medical SchoolActa Medica Okayama0386-300X5452000Characterization of epstein-barr virus-infected mantle cell lymphoma lines.193200ENZaishunJinNorihiroTeramotoTadashiYoshinoKenzoTakadaTakashiOkaKazuhikoHayashiTadaatsuAkagiArticle10.18926/AMO/32293<p>It has been reported that Epstein-Barr virus (EBV) resides in resting B cells in vivo. However, an ideal in vitro system for studying EBV latent infection in vivo has not yet been established. In this study, a mantle cell lymphoma line, SP53, was successfully infected with a recombinant EBV containing a neomycin-resistant gene. The EBV-carrying SP53 cells were obtained by selection using G418. They expressed EBER-1, EBNAs, and LMP1; this expression pattern of the EBV genes was similar to that in a lymphoblastoid cell line (LCL). However, proliferation assay showed that the EBV-carrying SP53 cells have a doubling time of 73 h, compared with 57 h of SP53 cells. Transplantation of 10(8) SP53 cells to nude mice formed tumors in 4 of 10 mice inoculated, but the EBV-carrying SP53 cells did not. Unexpectedly, EBV infection reduced the proliferation and tumorigenicity of SP53 cells. However, the EBV-carrying SP53 cells showed higher resistance to apoptosis induced by serum starvation than did the SP53 cells. The inhibition of proliferation and the resistance to apoptosis induced in SP53 cells by EBV infection indicate that this cell line might to some extent provide a model of in vivo EBV reservoir cells.</p>
No potential conflict of interest relevant to this article was reported.
Epstein-Barr virus
mantle cell lymphoma
latent infection
in vivo reservoir
SP53 line
Okayama University Medical SchoolActa Medica Okayama0386-300X4531991A monoclonal antibody (OPT1) to T cells which is available for paraffin-embedded materials.147154ENHiroshiMukuzonoTadashiYoshinoTadaatsuAkagiArticle10.18926/AMO/32209<p>A monoclonal antibody (MAb), OPT1, reactive with T cells in formalin-fixed, paraffin-embedded tissue sections, has been identified through immunization with activated T cells from peripheral blood lymphocytes (PBL). The antibody is an IgG1 antibody as demonstrated by the Ouchterlony technique. By cytofluorometric analysis, almost all CD3+ lymphocytes and only a few CD20+ lymphocytes of peripheral blood expressed the OPT1 antigen. Nonhematolymphoid cell lines were negative for OPT1 by the immunoperoxidase staining using acetone-fixed cell lines. On the contrary, peripheral T cells, cells of two T cell lines out of four and a part of the cells of one B cell line out of two were positive for OPT1. The immunoperoxidase staining of paraffin-embedded tissue sections revealed that most of lymphocytes in T cell areas of lymph nodes expressed OPT1 antigen. Some lymphocytes in both cortex and medulla of the thymus and erythroid precursors of the bone marrow were OPT1+. In the malignant lymphoma series, approximately 90% of T cell lymphomas and 6% of B cell lymphomas reacted with OPT1. None of the Reed-Sternberg cells nor Hodgkin cells in Hodgkin's disease were positive. Consequently, OPT1 may be useful for the diagnosis and study of malignant lymphomas and other related lesions.&#60;/P&#62;</p>
No potential conflict of interest relevant to this article was reported.
monoclonal antibody
OPTI
T cells
lymphocytes
lymphoma
Okayama University Medical SchoolActa Medica Okayama0386-300X4521991Effect of a long-acting somatostatin analogue (SMS 201-995) on a growth hormone and thyroid stimulating hormone-producing pituitary tumor.107115ENRyotoHirasawaHozoHashimotoShinyaMakinoShusoSuemaruToshihiroTakaoZensukeOtaYoshihikoHoshidaTadashiYoshinoTadaatsuAkagiArticle10.18926/AMO/32184<p>A 46-year-old woman with acromegaly and hyperthyroidism due to a pituitary adenoma. She had high serum thyroid-stimulating hormone (TSH) levels and very high serum growth hormone (GH) levels. Transsphenoidal removal of the tumor, post-operative irradiation, frontal craniotomy for removal of residual tumor and large-dose bromocriptine therapy were carried out consecutively. After therapy, serum GH levels gradually decreased, but not to the normal range, and serum TSH levels remained at inappropriately normal levels. Using immunoperoxidase techniques, GH-, TSH- and follicle-stimulating hormone (FSH)-containing cells were demonstrated in the adenoma. A long-acting somatostatin analogue (SMS 201-995, 600 micrograms/day) suppressed the serum GH level to the normal range with a concomitant suppression of TSH. Furthermore, the paradoxical serum GH responses to TRH and LH-RH were slightly improved. No important subjective side-effects were noted. Therefore, SMS 201-995 appeared to be a very effective drug in this patient with a GH- and TSH-producing pituitary tumor.&#60;/P&#62;</p>
No potential conflict of interest relevant to this article was reported.
TSH- and GH - producing pituitary adenoma
acromegaly
heperthyroidism
somatostatin analogue (SMS 201-995)
Okayama University Medical SchoolActa Medica Okayama0386-300X5822004Induction and prevention of virus-associated malignant lymphoma by serial transmission of EBV-related virus from cynomolgus by blood transfusion in rabbits.6774ENTirtha RajKoiralaKazuhikoHayashiZaishunJinSachiyoOnodaTakehiroTanakaWakakoOdaKoichiIchimuraNobuyaOharaTakashiOkaMasaoYamadaTadashiYoshinoArticle10.18926/AMO/32097<p>Epstein-Barr virus (EBV)-related herpesvirus (Si-IIA-EBV) was serially transmitted for 3 passages from rabbit to rabbit of the opposite sex by blood transfusion, which subsequently induced virus-associated rabbit lymphomas. The virus could be transmitted by transfusion with 15-20 ml of whole blood (7/7) or irradiated blood (1/6) from the EBV-related virus-infected rabbits, but there was no transmission with transfusion of cell-free plasma (0/6) from the infected rabbits. Passive anti-EBV-VCA IgG (x 20 approximately x 10) titers decreased during the first 1-2 weeks in the transfused rabbits. The virus-transmitted rabbits showed a gradual increase in antibody titers ranging from peak titers of x 640 to x 2560 after 3 weeks of transfusion. The recipient origin of malignant lymphoma that developed in the first rabbit transfused by infected blood was confirmed by chromosomal analysis. This rabbit model thus shows that EBV-related herpesvirus is serially transmissible by blood transfusion and that transmission can not be completely prevented by irradiation of blood, but removal of blood cells is the best way to prevent transmission of EBV-related virus. Therefore, this animal model provides a convenient in vivo system for studies of the prevention and therapy of transfusion-related transmission of EBV and EBV-associated lymphoproliferative diseases in immunocompromised human beings.</p>No potential conflict of interest relevant to this article was reported.
?Epstein-Barr virus(EBV)
rabbit
lymphoproliferative diseases
blood transfusion
Okayama University Medical SchoolActa Medica Okayama0386-300X5842004Expression and intracellular localization of FKHRL1 in mammary gland neoplasms.197205ENGui-ShanJinEisakuKondoTakayoshiMiyakeMasaoShibataTakakoTakashimaYi-XuanLiuKazuhikoHayashiTadaatsuAkagiTadashiYoshinoArticle10.18926/AMO/32088<p>FKHRL1 (FOXO3a), a member of the Forkhead family of genes, has been considered to be involved in the development of breast tumors; however, the in vivo expression and activation status of FKHRL1 in breast tumors still remains unclear. We immunohistochemically demonstrated the expression and intracellular localization of FKHRL1 in human breast tumors by the novel anti-FKHRL1 antibody which is available for formalin-fixed paraffin-embedded specimens. In a total of 51 cases of benign tumors, FKHRL1 was diffusely expressed in all cases, and its intracellular localization was revealed to be cytoplasmic (inactive form) in 94% of cases of intraductal papillomas (16/17) and 91% cases of fibroadenomas (31/34), with a similar pattern to normal glandular epithelium. In invasive ductal carcinomas, 83% of the cases (93/112) diffusely expressed FKHRL1; however, unlike benign tumors, 71% of the cases (66/93) showed the nuclear-targeted, active form of FKHRL1. Moreover, activated FKHRL1 was predominantly observed in scirrhous (29/36, 81% of the cases) and papillotubular (30/38, 79% of the cases) subtypes, compared to the solid-tubular subtype (7/19, 37% of the cases). Furthermore, the cases with nuclear-targeted FKHRL1 showed a tendency to have lymph nodal metastasis with statistical significance (P < 0.0001). Thus, the activation of FKHRL1 seems to be recognized as one of the specific features of invasive ductal carcinoma of the breast.</p>No potential conflict of interest relevant to this article was reported.
FKHRL1
intracellular localization
breast tumors
Okayama University Medical SchoolActa Medica Okayama0386-300X6332009Infusion of Hypertonic Saline into the Lung Parenchyma during Radiofrequency Ablation of the Lungs with Multitined Expandable Electrodes:Results Using a Porcine Model137144ENTatsuhikoIishiTakaoHirakiHidefumiMimuraHideoGobaraTaichiKuroseHiroyasuFujiwaraJunSakuraiHiroyukiYanaiTadashiYoshinoSusumuKanazawaOriginal Article10.18926/AMO/31848<p>The present study was performed to clarify the effect of hypertonic saline infusion into the lung parenchyma on radiofrequency ablation (RFA) of the lungs. A total of 20 ablation zones were created in 3 pigs. The ablation zones were divided into 3 groups. Group 1 (n6) consisted of ablation zones created by applying smaller radiofrequency (RF) power without saline infusion;group 2 (n5) zones were created by applying greater RF power without saline infusion;and group 3 (n9) zones were created by applying greater RF power with saline infusion. The techniques of saline infusion included administration of hypertonic saline 1ml before RFA, followed by continuous administration at a rate of 1ml/min during the first 2min after the initiation of RFA. The ablation parameters and coagulation necrosis volumes were compared among the groups. Group 3 had a tendency toward smaller mean impedance than group 1 (p0.059) and group 2 (p0.053). Group 3 showed significantly longer RF application time than group 2 (p0.004) and significantly greater maximum RF power than group 1 (p0.001) and group 2 (p0.004). Group 3 showed significantly larger coagulation necrosis volume (mean, 1,421mm3) than group 2 (mean, 858mm3, p0.039) and had a tendency toward larger necrosis volume than group 1 (mean, 878mm3, p0.077). Although this small study had limited statistical power, hypertonic saline infusion during RFA appeared to enlarge coagulation necrosis of the lung parenchyma.</p>No potential conflict of interest relevant to this article was reported.
radiofrequency ablation
lung
experimental study
Okayama University Medical SchoolActa Medica Okayama0386-300X6322009Expression of thyroglobulin on follicular dendritic cells of thyroid mucosa-associated lymphoid tissue (MALT) lymphoma7178ENMitsuruMunemasaTadashiYoshinoKeitaKobayashiTakayoshiMiyakeSumie TakaseSakugawaTomohikoMannamiKatsujiShinagawaMitsuneTanimotoTadaatsuAkagiOriginal Article10.18926/AMO/31834<p>Reportedly, thyroid mucosa-associated lymphoid tissue (MALT) lymphoma is closely associated with Hashimoto's thyroiditis. However, it remains unknown which antigen is closely associated with thyroid MALT lymphoma. We examined whether B cell response to thyroglobulin (Tg), which is a common thyroid-specific autoantigen, is related etiologically to the pathogenesis of thyroid MALT lymphoma. Expression of human Tg antigens and Cluster of differentiation (CD) 35 was examined immunohistochemically in 15 cases of thyroid MALT lymphoma using paraffin-embedded, formalin-fixed tissue specimens. In all cases of thyroid MALT lymphoma, human Tg was detected immunohistochemically in the follicular epithelial cells and follicular dendritic cells (FDCs). These FDCs were positive by double immunostaining for anti-human Tg rabbit polyclonal antibody (Ab) and for CD35. Results showed that the Tg, a thyroid autoantigen, had immunostained the germinal center of the thyroid MALT lymphoma. The Tg was present in the FDCs, as revealed by the staining pattern of the germinal center;this fact was confirmed by double immunostaining of anti-human Tg mouse monoclonal Ab and anti-CD35 mouse monoclonal Ab. The results of our study suggest that Tg is an autoantigen that is recognized by thyroid MALT lymphoma cells.</p>No potential conflict of interest relevant to this article was reported.
thyroglobulin
follicular dendritic cells
mucosa-associated lymphoid tissue lymphoma
Okayama University Medical SchoolActa Medica Okayama0386-300X4111987Metaplastic bone formation in a hyperplastic polyp of the stomach: a case report.4346ENYujiOhtsukiYoshifumiDanbaraIsaoTakedaKiyoshiTakahashiKazuhikoHayashiHiroshiSonobeTadashiYoshinoTadaatsuAkagiArticle10.18926/AMO/31765<p>Metaplastic bony tissue along with hyperplastic mucosal epithelium showing no atypism was detected in biopsy materials from a Yamada type I gastric polyp. The tissue was metaplastic woven bone associated with calcification. Histogenesis of the bone formation is as yet unknown. This is the first reported case of the presence of metaplastic bone accompanied by hyperplastic gastric mucosa so far.</p>
No potential conflict of interest relevant to this article was reported.
stomach
hyperplastic polyp
metaplastic bone
histopathology
Okayama University Medical SchoolActa Medica Okayama0386-300X5311999Hydrocortisone Sodium Succinate Suppressed Production of Interleukin-10 by Human Peripheral Blood Mononuclear Cells: Clinical Significance5559ENHideoKohkaHiromiIwagakiTadashiYoshinoKentaKobashiShinnyaSaitoHiroshiIsozakiNorihisaTakakuraNoriakiTanakaArticle10.18926/AMO/31646<p>Corticoids are well known for their immunosuppressive properties. Interleukin-10 (IL-10) is an intrinsic antiinflammatory peptide in immune diseases, originally identified as cytokine synthesis inhibitory factor. We examined the effect of hydrocortisone sodium succinate (HSS) on the production of IL-10 by human peripheral blood mononuclear cells (PBMCs). PBMCs from healthy volunteers and cancer-burden patients were preincubated separately with or without HSS for 1 h, then stimulated with 5 microg/ml lipopolysaccharide (LPS). Production of IL-10 by human PBMCs was detected with LPS stimulation and its production was higher in cancer-burden patients than in normal volunteers, although this was not statistically significant. HSS suppressed production of IL-10 by LPS-stimulated PBMCs in a dose-dependent manner both in normal volunteers and in cancer-burden patients. These results indicate that, in addition to their antiinflammatory properties, corticoids act to restore the immunosuppressive states even in cancer-burden states</p>
No potential conflict of interest relevant to this article was reported.
steroid
interleukin-10
cancer-burden state
Okayama University Medical SchoolActa Medica Okayama0386-300X4711993Distribution of Lectin Receptors in the Human Hyperplastic Tonsil: Histochemical and Flow Cytometric Analyses1319ENAshit BaranSarkerTadaatsuAkagiTadashiYoshinoKotaroFujiwaraSoichiroNoseArticle10.18926/AMO/31606<p>The distribution of lectin receptors in the human tonsil was studied using 16 biotinylated lectins. The avidin-biotin-peroxidase complex (ABC) method was used on frozen and paraffin-embedded tissue sections. Cell suspensions were also analysed by dual flow cytometry using respective fluorescein isothiocyanate-conjugated lectins and phycoerythrin-labeled anti-CD3 and anti-human immunoglobulin. Frozen sections fixed with acetone and paraffin-embedded materials fixed in three solutions were compared for lectin affinity; ethanol-fixed sections gave best results followed by frozen and buffered formalin-fixed ones, then nonbuffered formalin. Con-A, RCA-1, LcH, WGA, MPA, PHA, PSA, PNA, SJA and GSA-1 reacted with all tissue components of the tonsil in immunohistochemical studies, but binding intensity was fixative dependent. Binding of Lotus and BPA to lymphocytes was limited to germinal center lymphocytes. Other tissue components were also reactive but staining intensity was weaker in Lotus compared with BPA. SBA and DBA did not react with lymphocytes, but reacted with macrophages/histiocytes, vascular endothelia, and epithelial cells. LBA and LPA were constantly negative with all tissue components irrespective of fixatives. Flow cytometric analyses showed that all but three (DBA, LBA and LPA) partially or totally stained lymphocyte surfaces. Lotus receptors were expressed exclusively on B-lymphocytes.</p>
No potential conflict of interest relevant to this article was reported.
lectins
?histochemistry
flow cytometry
human tonsil
Okayama University Medical SchoolActa Medica Okayama0386-300X4731993Expression of a lymphocyte adhesion molecule (CD44) in malignant lymphomas: relevance to primary site, histological subtype and clinical stage.215222ENKotaroFujiwaraTadashiYoshinoKenjiMiyakeNobuyaOharaTadaatsuAkagiArticle10.18926/AMO/31588<p>Lymphocyte adhesion molecules defined by anti-CD44 antibody (Hermes-3) may be involved in lymphocyte binding to high endothelial venules at sites where lymphocytes exist the blood. CD44 expression was immunohistochemically examined in 167 well characterized cases of malignant lymphomas (MLs). None of 12 nodal follicular lymphomas (FLs) were CD44+, whereas 3 of 4 extranodal ones showed distinct CD44 expression. In contrast to nodal FLs, 28 of the 38 (74%) nodal diffuse B-cell lymphomas were CD44+ (p < 0.0001). T-cell lymphomas showed a significantly higher expression of CD44 antigen than diffuse B-cell lymphomas in the nodal cases (p < 0.04), but not in the extranodal ones. In nodal diffuse lymphomas, 3 of 5 stage I lymphomas (60%) were CD44+ in contrast to 53 of 63 stage II-IV lymphomas (84%), but the difference was not statistically significant. Of 14 Hodgkin's diseases, 9 cases were CD44+ with no significant correlation with clinical stage. The data of flow cytometric analysis confirmed the results of immunohistochemical analysis. In conclusion, CD44 expression is relevant to primary sites of distinctive MLs originating in the mucosal regions (MALToma) and some histological subtypes, but the relation with clinical stage was not defined. Some other adhesion molecules or different mechanisms must also be taken into account concerning the genesis and the expansion of MLs.</p>No potential conflict of interest relevant to this article was reported.
malignant lymphomas
adhesion molecules
CD44
clinical staging
histological classification
Okayama University Medical SchoolActa Medica Okayama0386-300X4831994Endoscopical Segmental Piecemeal Tumorectomy for Nodular Elevation of Colorectal Tumor: Applicability and Patient's Quality of Life169171ENNorifumiArikiHiromiIwagakiTadashiYoshinoYasuyukiNonakaShigeatsuFujikiJose AntonioPerdomoAkioHizutaJunTomodaNoriakiTanakaTakaoTsujiKunzoOritaArticle10.18926/AMO/31124<p>Endoscopical segmental piecemeal tumorectomy (ESPT) for nodular elevation of colorectal tumor is advantageous in terms of minimizing both surgical invasion and postoperative burden to the patients. Nodular elevation of colorectal tumors is said to occur when the body of the tumor is adenomatous and the surface of the focal cancer grows more horizontally into the lumen than vertically. We report here four cases of nodular elevation of colorectal tumors which were each treated by different surgical procedures.</p>
No potential conflict of interest relevant to this article was reported.
nodular elevation
coloretal tumors
endoscopical segmental piecemeal tumorectomy
Okayama University Medical SchoolActa Medica Okayama0386-300X4821994Partial Purification and Chraracterization of Dendritic Cell Differentiation Factor6772ENKatsuyaMiyataniKiyoshiTakahashiHiroyukiYanaiTadashiYoshinoTadaatsuAkagiArticle10.18926/AMO/31108<p>Previously, we reported that interleukin-2 (IL-2)-stimulated helper T cells produced an unknown soluble factor which induced dendritic cell-like differentiation in primary cultures of monocytic leukemia cells and we referred to this factor as dendritic cell differentiation factor (DCDF). In this study, we attempted to purify and characterize DCDF and investigated its biological effect on normal human monocytes. Gel filtration chromatography indicated that the molecular weight of DCDF is approximately 30-35 kDa. Chromatofocusing indicated that the isoelectric point of DCDF is approximately 5.0. DCDF, partially purified by subsequent gel filtration, chromatofocusing, and hydrophobic chromatography, significantly enhanced the HLA-DR expression of normal human monocytes and a human monocytic leukemia cell line, THP-1. This biological activity was not neutralized by any known antibodies to human cytokines. DCDF significantly amplified the T-cell stimulatory activity of monocytes in the allogeneic mixed leukocyte reaction (MLR). Moreover, DCDF significantly enhanced IL-1 beta and IL-6 production by monocytes in a dose-dependent manner. These results suggest that DCDF is a novel human cytokine which stimulates the accessory cell function of monocytes.</p>
No potential conflict of interest relevant to this article was reported.
dendritic cell
differentiation
protein purification
cytokine
Okayama University Medical SchoolActa Medica Okayama0386-300X4841994Application of polymerase chain reaction (PCR) to the microscopically identified cells on the slides: evaluation of specificity and sensitivity of single cell PCR.189193ENNorihiroTeramotoYujiTonoyamaTadaatsuAkagiAshit BaranSarkerTadashiYoshinoIchiroYamadoriKiyoshiTakahashiArticle10.18926/AMO/31091<p>The sensitivity and specificity of single cell polymerase chain reaction (PCR) were studied. Its high sensitivity enabled detection of a single-copy gene, such as human T-lymphotropic virus type I genome in paraffin sections. The rate of obtaining positive signals with this method was affected by the number of copies of the gene in the target cell. Specificity was satisfactory if the procedure was properly and carefully followed. Since the single cell PCR is a time-consuming method which requires skill and experience to pick up the target cells accurately, the applicability of this method is limited. It works best when it is used to analyze a single or a few copy genes in histologically identified cells.</p>
No potential conflict of interest relevant to this article was reported.
polymerase chain reaction
human T-lymphotropic virus type I
paraffin section
single cell
single copy gene
Okayama University Medical SchoolActa Medica Okayama0386-300X4331989Immortalization of rat spleen and thymus T cells by human T-cell leukemia virus type I.143151ENTadaatsuAkagiHiroshiTakataTadashiYoshinoNorihiroTeramotoShokiYanoTakashiOkaArticle10.18926/AMO/30886<p>Co-cultivation of thymus and spleen cells of Fisher and Lewis rats with lethally irradiated MT-2 cells harboring human T-cell leukemia virus type I (HTLV-I) resulted in the establishment of lymphoid cell lines, FIRT-1, FIRS-1, LERT-1, and LERS-1, respectively. Cells of these cell lines had rat T-cell characters as demonstrated by the positive reaction to monoclonal antibodies (MAbs) to rat T cell antigens (Thy 1 and pan T). They lacked surface immunoglobulins and strongly expressed rat interleukin-2 receptor antigen (Tac) and Ia antigen. Karyotypic analysis revealed that they had the normal rat karyotype in early cultures, but showed marked aneuploidy after long cultivation. None of them expressed HTLV gag proteins (p19 and p24) or virus particles, but they contained HTLV-I proviral DNA monoclonally and weakly expressed pX gene products (p40x). They were not transplantable into syngeneic newborn rats.</p>
No potential conflict of interest relevant to this article was reported.
human T-cell leukemia virus
rat T cell
immortalization
Okayama University Medical SchoolActa Medica Okayama0386-300X4341989Ontogeny of S-100 protein-positive histiocytes and lymphocytes in the human fetal lymphoreticular system.203210ENTadaatsuAkagiSoichiroNoseKiyoshiTakahashiTadashiYoshinoYasushiHorieMakotoMotoiHiroshiSonobeHideakiEnzanArticle10.18926/AMO/30854<p>In the human lymphoreticular system, the alpha and beta subunits of S-100 protein are found in ordinary monocyte-macrophages and non-phagocytic histiocytes such as Langerhans cells and interdigitating reticulum cells, respectively. The beta subunit is also present in some CD8+ T cells. In the present study, we investigated the ontogeny of these histiocytes and lymphocytes in humans. Yolk sacs and 4 to 21-week fetuses were examined immunohistochemically for the presence of S-100 protein subunits using antisera monospecific to each subunit. S-100 alpha + macrophages were present in the yolk sacs and the hepatic sinusoids of the 4th week embryos prior to bone marrow hematopoiesis. These macrophages later appeared in other lymphoid organs when anlagen of these organs were formed. No S-100 beta + cells were found in the yolk sacs. S-100 beta+ histiocytes were first detected in the hepatic sinusoids of the 5th week embryo, and after the 8th week of gestation, they were distributed in other lymphoid organs. S-100 beta+ lymphocytes were not found in the liver. They were first detected in the thymus at the 12th week of gestation, and were subsequently distributed in other lymphoid organs. These results suggest that S-100 beta+ lymphocytes and histiocytes may belong to different cell lineages, and the former may not be the precursor of the latter.</p>
No potential conflict of interest relevant to this article was reported.
S-100 protein
ontogeny
lymphocyte
histiocyte
Okayama University Medical SchoolActa Medica Okayama0386-300X5141997Analysis of the genome of an Epstein-Barr-virus (EBV)-related herpesvirus in a cynomolgus monkey cell line (Si-IIA)207212ENHideoInoKazuhikoHayashiHiroyukiYanaiNorihiroTeramotoTirtha RajKoiralaHong-LiChenTakashiOkaTadashiYoshinoKiyoshiTakahashiTadaastuAkagiArticle10.18926/AMO/30764<p>A simian cell line, Si-IIA, harboring Epstein-Barr-virus (EBV) -related herpesvirus (Si-IIA-EBV), produces malignant lymphoma in rabbits when administered by intravenous inoculation. In this study, we analyzed the Si-IIA-EBV genome and compared it with human EBV and herpesvirus macaca fascicularis 1 (HVMF 1 ), which is associated with B-cell lymphoma developing in SIV-infected immunosuppressed monkeys. DNA from Si-IIA-EBV was amplified by the polymerase chain reaction using three different primer pairs complementary to human EBV (B95-8) DNA; two of the primer pairs covered part of the long internal repeat 1 region (IR 1) and the third covered part of the BRRF 1 region. Direct sequencing of the three PCR products revealed that Si-IIA-EBV DNA had about 82% nucleotide homology to the human EBV DNA in all three regions and 92.4% homology to HVMF1 in the IR1 region. The blotting pattern by Southern blot analysis was different between Si-IIA-EBV and human EBV.</p>
No potential conflict of interest relevant to this article was reported.
Epstein-Barr virus
HVMF 1
lymphoma
?monkey cell line
PCR
Okayama University Medical SchoolActa Medica Okayama0386-300X5031996Regulation of Interleukin-2 Receptor y Chain mRNA Expression in Human Monocytic Cell Line THP-1145150ENHiroyukiYanaiTadashiYoshinoKiyoshiTakahashiYoshifumiNinomiyaTadaatsuAkagiArticle10.18926/AMO/30509<p>Circulating hepatitis C virus (HCV) particles can be fractionated by means of differential flotation centrifugation. It is reported that in the bottom fraction HCV is in the form immune complexes, whereas in the top, it is free of antibodies. We evaluated the significance of circulating complex and free HCV in chronic hepatitis C, and assessed the relationship in terms of the response to interferon (IFN) therapy. We examined sera before, just after, and 1 year after administering IFN to 18 patients with chronic hepatitis C, 10 of whom responded (group CR), and 8 did not (group NR). The amounts of virus were similar between both groups before therapy. After differential flotation centrifugation with 1.063 g/ml of NaCl, the top and bottom fractions were assayed for HCV RNA. Before therapy, HCV RNA was detected in the top fraction in 1 of 10 in group CR, and in 6 of 8 in group NR (P &#60; 0.05, chi-square test). HCV RNA was positive in the bottom fraction of all samples. In a follow-up study of group NR, HCV RNA was detected in the top fraction in 3 of 8 just after IFN therapy, and in 7 of 8 after 1 year. This study suggests that the presence of HCV in the top fraction can predict a poor response to IFN therapy.</p>
No potential conflict of interest relevant to this article was reported.
IL-2R ??chain
phorbol ester
monocyte
differentiation
protein kinase
Okayama University Medical SchoolActa Medica Okayama0386-300X5051996Variable expression of Epstein-Barr virus latent membrane protein I in Reed-Sternberg cells of Hodgkin's disease.267270ENNorihitoTeramotoliuCaoNobuhiroKawasakiYujiTonoyamaAshit BaranSarkerTadashiYoshinoKiyoshiTakahashiTadaatsuAkagiArticle10.18926/AMO/30493<p>&#60;P&#62;Reed-Sternberg cells (RS cells) of Hodgkin's disease (HD) are frequently infected with Epstein-Barr virus (EBV) and express EBV-encoded nonpolyadenylated RNA transcripts (EBER)-1. EBV latency has been classified into three distinct forms: Latency I, expressing only one of the latent proteins, EBV nuclear antigen (EBNA)-1, latency II, coexpressing EBNA-1 and LMPs, and latency III, expressing all latent viral proteins. RS cells express LMP-1 in addition to EBNA-1 and are considered to be EBV latency II frequently encountered in nasopharyngeal carcinoma. We examined 13 cases of EBV-infected HD by combined EBER-1 in situ hybridization and immunostaining for LMP-1. All of the RS cells expressed EBER-1, but a substantial number of EBER-1+ RS, cells were negative for LMP-1. The percentage of LMP-1+ RS cells out of EBER-1+ RS cells varied from 7% to 100% (average 69%). In this study, we showed that all EBV-infected RS cells were not restricted to latency II, and some belonged to latency I.&#60;/P&#62;</p>
No potential conflict of interest relevant to this article was reported.
in situ hybridization
EBER-1
immunohistochemistry
latecy
Okayama University Medical SchoolActa Medica Okayama0386-300X5021996Detection of Epstein-Barr virus RNA and related antigens in non-neoplastic lymphoid lesions.8996ENYujiTonoyamaNorihiroTeramotoAshit BaranSarkerTadashiYoshinoKazuhikoHayashiKiyoshiTakahashiTadaatsuAkagiArticle10.18926/AMO/30484<p>To elucidate the latent state and reactivation of Epstein-Barr virus (EBV) in non-neoplastic lymphoid lesions, we investigated 144 non-neoplastic lymphoid lesions by in situ hybridization (ISH) to detect the expression of EBV-encoded small RNAs (EBER)-1 and BCRF-1 and by immunostaining for latent membrane protein (LMP)-1 and ZEBRA. ISH for EBER-1 detected EBER-1-positive cells (EPC) in 31 of the 144 examined lesions (22%). EPC were detected in 4 of 49 cases of nonspecific lymphoid hyperplasia, in 16 of 20 abscess-forming granulomatous lymphadenitis (AFGL), 5 of 25 Kikuchi's disease, and in 3 of 3 infectious mononucleosis. LMP-1 was expressed in 6 of 124 non-neoplastic lymphoid lesions (4.8%). LMP-1-positive cells were observed in 6 of the 31 EBER-1-positive cases (19%). EPC were detected significantly more frequently in LMP-1- and ZEBRA-positive specimens than in the LMP-1- and ZEBRA-negative specimens. BCRF-1 was expressed in 4 of 11 cases examined: 2 of 3 AFGL, 1 of 2 Kikuchi's disease, and in the 1 case of atypical lymphoid hyperplasia. This study suggests that Epstein-Barr virus is prevalent and can be reactivated in the lymph nodes effaced by destructive inflammation, such as AFGL. Such inflammation may provide a local milieu that is conducive for EBV to enter the lytic cycle.</p>No potential conflict of interest relevant to this article was reported.
EBER-I
BCRF-l
LMP-l
ZEBRA
lymphoid lesion
Okayama University Medical SchoolActa Medica Okayama0386-300X4451990Primary non-Hodgkin's lymphoma of the rectum: a case report.279282ENLuis FernandoMoreiraHiromiIwagakiKazuhikoWatanabeTadashiYoshinoSadanoriFuchimotoKunzoOritaArticle10.18926/AMO/30452<p>A rare gastrointestinal tract neoplasm, primary non-Hodgkin's B-cell lymphoma in a 39-year-old, asymptomatic woman is described. The tumor was originally localized in the rectum without evidence of any other lymphoma-involved organ and treated by curative surgical procedure associated with postoperative chemotherapy.</p>
No potential conflict of interest relevant to this article was reported.
primary lymphoma
rectum
surgical treatment
Okayama University Medical SchoolActa Medica Okayama0386-300X4451990Comparison of monoclonal antibodies reactive with lymphocyte subsets in routinely fixed paraffin-embedded material: flow cytometric analyses, immunoperoxidase staining and influence of fixatives.243250ENTadashiYoshinoYoshihikoHoshidaIchiroMurakamiKiyoshiTakahashiTadaatsuAkagiArticle10.18926/AMO/30447<p>We have attempted to clarify the characteristics of monoclonal antibodies (MAbs) detecting lymphocyte subsets in fixed materials. We examined by means of flow cytometric technique influences of fixatives and reactivity with malignant lymphomas (MLs). Specific markers for T-cells were UCHL1 and OPD4, which reacted especially with helper/inducer T-cells. MT1 recognized almost all of T-cells from peripheral blood and tonsils, but reacted with a part of B-MLs. As for B-cell markers, L26 was the most reliable marker for B-MLs. L26 and MB1 antigens could not be detected on living cells flow cytometrically. LN1 reacted with a part of T-cells as well as B-cells, but fluorescent intensity of the former was apparently stronger than that of the latter. Although LN2 antigen was located mainly in the cytoplasm close to the nuclear membrane immunohistochemically, it could be detected on living cells flow cytometrically. LN2 positive cells belonged to B-cells in peripheral blood and tonsils. When fixed for relatively short time, B5 and buffered formalin were better for examining MAbs than non-buffered formalin and ethanol.</p>
No potential conflict of interest relevant to this article was reported.
monoclonal antibodies
lymphocyte subset
flow cytometry
Okayama University Medical SchoolActa Medica Okayama0386-300X3821984Immunohistochemical demonstration of lysozyme in normal, reactive and neoplastic cells of the mononuclear phagocyte system.125133ENMakotoMotoiTadashiYoshinoKenjiKawabataIkukoIkeharaShozoOhsumiKatsuoOgawaArticle10.18926/AMO/30333<p>Using the peroxidase antiperoxidase (PAP) method, lysozyme (LZM) was shown to exist in normal, reactive and neoplastic cells belonging to the mononuclear phagocyte system (MPS), but was not detected in histiocytosis X cells. Immunostaining for cytoplasmic LZM by the PAP method is useful for identification of mononuclear phagocytes and for diagnosis of the diseases in which these cells participate.</p>
No potential conflict of interest relevant to this article was reported.
lysozyme
PAP method
mononuclear phagocyte system
岡山医学会Acta Medica Okayama0030-155812212010IgG4 関連疾患7779ENYasuharuSatoTadashiYoshinoNo potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-155812032008ラット中脳動脈閉塞・再灌流モデルにおける抗 HMGB1 単クローン抗体の治療効果271277ENKeyueLiuShujiMoriHideoTakahashiYasukoTomonoHidenoriWakeToruKankeYasuharuSatoNorihitoHiragaNaotoAdachiTadashiYoshinoMasahiroNishiboriNo potential conflict of interest relevant to this article was reported.
抗体医薬
炎症
HMGB1
脳梗塞
血液脳関門
岡山医学会Acta Medica Okayama0030-155811312001胃 MALTリンパ腫：基礎8791ENNo potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030155811932008エピジェネティクス323325ENKatsuyoshiTakataTadashiYoshinoNo potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030155811922007非小細胞肺癌における癌・精巣抗原XAGE-1の発現と免疫応答の解析127129ENKazuhikoNakagawaYujiNoguchiHideoOkumuraShuichiroSatoMotoyukiTanakaMichihideShimonoAli MohamedAli EldibMotoiAoeToshiroOnoAkikoUenakaNobuyaOharaTadashiYoshinoKazukiYamashitaTsukasaTsunodaNobuyoshiShimizuEiichiNakayamaNo potential conflict of interest relevant to this article was reported.
XAGE-1b
癌精巣抗原
非小細胞肺癌
Acta Medica Okayama1985Morphological Maturation of Tumor Cells Induced by Ethylnitrosourea (ENU) in Rat Brains 第1編 On the Tumors by Administration of ENU in the Late Gestational Stage 第2編 On the Tumors by Administration of ENU in the Midgestational StageENNo potential conflict of interest relevant to this article was reported.