Piccolo is an optional Pixl in the game Super Paper Mario. It takes some time to run a series of errands to earn Piccolo. Piccolo plays music when the player press "1" on the Wii Remote. She can play music that can cure Mario and the team from any curses placed upon them by Cursya. If the player switches characters while Piccolo is equipped, the player will hear different tunes. She plays Italian songs for Mario and Luigi, a cheery lullaby for Peach, and a metal rock theme for Bowser. Any of these themes can put the Underchomp to sleep.

Some blocks have a Piccolo symbol on them. By using Piccolo when standing next to them, the blocks dissolve, revealing a treasure chest. You can get her after you finish Chapter 5.