PRIMER WALKING

Is your freezer full of unmapped or mystery constructs? Do your transformations grow on the wrong antibiotic resistance plate? GENEWIZ Primer Walking service is designed for situations when you need to determine the sequence of a completely unknown plasmid or PCR product.

Our Service Includes

Primer design and synthesis

Plasmid preparation or RCA (if needed)

Single-stranded or double-stranded coverage per base pair as dictated by project requirements

Project Deliverables

SEQUENCE CONFIRMATION SERVICE

Do your digestions show incorrect banding patterns? Does your construct fail to sequence or amplify with the anticipated primer set? GENEWIZ Sequence Confirmation service is designed for situations when you need to verify the identity of your construct with single- or double-stranded certainty.

Our Service Includes

Primer design and synthesis

Plasmid preparation or RCA (if needed)

Single-stranded or double-stranded coverage per base pairs as dictated by project requirements

Manual editing based on sequence chromatogram

Sequence data assembly for consensus sequence

Consensus report

Upon Order Submission

Please send to GENEWIZ:

Template, in tubes or plates

Reference sequence, in .fasta, .txt. or .seq format

Requested number of base pairs sequenced

Single-stranded or double-stranded coverage requirements

Project Deliverables

Standard turnaround time starting at 5-7 business days

Consensus sequence, in .seq or .fasta format

Variance report, if applicable

All raw sequencing data, in .ab1 and .seq formats

Any size plasmid prep, upon request

HOW TO ORDER

*Samples must arrive at the GENEWIZ New Jersey Laboratory before 10:00 am EST to qualify for Same Day service. Note that direct-sequencing templates are not available for our Same Day service.