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GEA Niro Method No. A 18 a

Revised: September 2005

1.Definition

The content of a-lactose and total lactose is determined on the basis of 2 polarimetric readings.

2.Scope

The method can be used on milk and whey powder.

3.Principle

The proportion of a-lactose (of total lactose) is determined on the basis of 2 polarimetric readings. The first optical rotation of the clear filtrate is taken under such conditions that the temperature of the solution is kept low during the whole procedure to prevent mutarotation. The second reading is made after the equilibrium has been reached.

The analysis must be carried out in a cold room, with a temperature not higher than 5°C.