EPA's oral reference dose (daily oral exposure likely to be without an appreciable risk of deleterious effects during a lifetime) for cyclohexanone is 5 mg/kg/day.

Two cases of contact dermatitis due to cyclohexanone exposure have been reported.

In a study with human volunteers who inhaled cyclohexanone for 8 hours, the major urinary metabolites over 72 hours were found to be 1,2-cyclohexanediol (38-41% of dose) and 1,4-cyclohexanediol (18-21% of dose), including their glucuronide conjugates. These metabolites appeared to be useful for biomonitoring exposure to cyclohexanone.