*Newest sequencing results for T/R lab using primers NO282, 283, and 296 are available from Genewiz (look at order # 10-180371918). Reactions using the newest primer (NO297) will be sent on Monday and should be ready for you to look at on Tuesday.

*Extra office hours with Linda Sutliff are posted to [[User:Linda L. Sutliff| her user page.]] Drop in or schedule a time--but try to take advantage of this resource!

*Extra office hours with Linda Sutliff are posted to [[User:Linda L. Sutliff| her user page.]] Drop in or schedule a time--but try to take advantage of this resource!

*The [[Talk:20.109%28F11%29:_Mod_2_Day_6_Readouts_of_DNA%2C_Protein | M2D6 Talk]] page has a place to share your data in summary form. By sharing, we can look for trends in the class results.

*The [[Talk:20.109%28F11%29:_Mod_2_Day_6_Readouts_of_DNA%2C_Protein | M2D6 Talk]] page has a place to share your data in summary form. By sharing, we can look for trends in the class results.

Welcome to 20.109! For many of you this will be the first time in a research lab and for others it will not, but it is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. There is not time enough to show you everything you’ll need to know if you go on to do research, but after taking this class you should feel confident and familiar with some fundamental experimental approaches and lab protocols. You will develop good habits at the bench, ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with report writing, notebook keeping and publicly presenting your data. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

Announcements

Newest sequencing results for T/R lab using primers NO282, 283, and 296 are available from Genewiz (look at order # 10-180371918). Reactions using the newest primer (NO297) will be sent on Monday and should be ready for you to look at on Tuesday.

Extra office hours with Linda Sutliff are posted to her user page. Drop in or schedule a time--but try to take advantage of this resource!

The M2D6 Talk page has a place to share your data in summary form. By sharing, we can look for trends in the class results.

FINISHING UP MODULE 1: Your ppts should be back from Bevin at the start of next week, 11.7.11!

Agi will hold office hours Monday and Thursday of next week from 2-3:30 pm in room 16-336.

NK will hold office hours on Sunday 4PM in lab and on Thursday 11/10 at 11AM in lab.

W/F sequencing data is now available. Note that you must log in as "astachow" rather than "nkuldell" (at mit...) and otherwise the M2D6 instructions apply.

Agi's usual 2-3 pm office hours are today (in 16-319). All are welcome. W/F section, you can pick up your quizzes there, and must hand in any late lab notebooks by then.

T/R sequencing data is available to examine on GENEWIZ site. Instructions for accessing data are detailed in M2D6 protocol.

Breaking news: library concentration is 0.4 ug/ul. Sorry this wasn't on M2D3 originally (it is now!)

Howard Silver's notes about references are linked to the schedule page for M2D5

W/F data has been added to the M2D2 Talk page. I have left space for each team to note any irregularities (A420 out of linear range, realized you didn't mix cells well, Δt was really short, etc.) or other relevant information.