Description:
Human papillomavirus (HPV) is a diverse group of DNA-based viruses that infect skin and mucous membranes of humans and animals. Some HPV types are the causative agents of cervical cancer, with types 16 and 18 being particularly high-risk. The viral proteins E6 and E7 disrupt normal cell cycle regulation by interacting with p53 (a tumor-suppressing transcription factor) and Rb (retinoblastoma protein, also a tumor-supressor). E7 particularly binds to Rb and histone deacetylases, resulting in activation of the E2F genes, which code for a family of transcription factors. The viral proteins E6 and E7 might be of particular interest in the development of therapeutic vaccines, since they are expressed early in viral infection.

Applications: Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:ELISA: 1:20-1:200Western Blot: 1:10-1:50Optimal dilutions to be determined by the researcher.

Recommended Pair:Pairs with P3105-19

Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type

Isotype

Clone

Grade

Mab

IgG2a

5E392

Affinity Purified

Size

Storage

Shipping

Source

Host

1ml

-20°C

Blue Ice

Mouse

Concentration:

~0.1mg/ml

Immunogen:

Papilloma Virus, Human, Type 16/18, E7.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins have been added.

Specificity:

Recognizes the E7 protein of HPV types 16 & 18.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.