Figure 6.

IGF1-induced bone growth partially requires PI3K activity. (A)Tibiae isolated from E15.5 mice were cultured for 6 days in the presence of IGF1
or 0.1% BSA in PBS, as control. IGF1 induced significant increase in bone growth.
(B) Measurements of bone growth after 6 days of treatment with IGF1, control, LY294002
or IGF1 + LY294002 showed increased bone growth in the IGF1 treatment, and similar
bone growth between the control and the IGF1 + LY294002 treatment. (C) E 15.5 tibiae
treated for 6 days with the treatments mentioned above, were fixed, embedded, sectioned
and stained with Safranin O. The length of hypertrophic zone was increased in the
IGF1 treatment compared to control; decreased in the LY294002 treatment as shown before
and in the case of the IGF1 + LY294002 treatment similar to the control hypertrophic
zone length.