"Developed over many years, these media provide the basic nutrients for cell growth but may lack the additional factors that promote higher levels of cell proliferation and product expression. Classical media are those that require supplementation with serum, protein fractions, lipid mixes, or some combination thereof. They have been the basis of most of the original bioproduction processes. Typical classical media include D-MEM, RPMI, BME, MEM, Hamís F12 and similar basal formulations."