TABLE OF CONTENTS:

Purpose of Study:

Demonstrate ability of the Hand Sanitzer Lotion with Invisicare to adhere to skin under conditions that simulate normal working conditions. Reveal the Hand Sanitzer Lotion with Invisicare ability to persist on skin throughout multiple hand washes and exposure to detergents, chemicals and solvents.

Testing Laboratory:

Protocol:

The Hand Sanitzer Lotion with Invisicare was applied to fritted glass membranes to simulate a normal application on skin. The membranes were subjected to an agitated 5% Ammonium Hydroxide wash with tests being conducted over six hours. Gravimetric analysis was used to determine the level of adherence to the glass membranes. An additional test with artificial perspiration yielded no change in persistence.

Testing Laboratory:

Protocol:

Utilizing 30 ethnically diverse human subjects, the Hand Sanitzer Lotion with Invisicare was applied to the volar forearm, and then scrubbed with gauze saturated with 5% Ammonium Hydroxide at intervals of 10 minutes, 2 hours and 4 hours. The same gravimetric analysis as used in the in vitro studies, were performed on the skin wash solutions. Throughout the four-hour test period, punch biopsies were harvested from random subjects for examination under Electron Microscopy and Atomic Force Microscopy.