Why do we usually use ethanol 100% first then 70% in DNAYou have 1 L of 100 proof (50% V/V) Scotch whisky (ethanol, #C_2H_5OH#). Calculate the molarity, the mole fraction, the molality of the ethanol and if the temperature drops to …... in many protocol for DNA extraction I have seen that 100% ethanol (absolute ethanol) is used first for DNA precipitation (washing) then 70% ethanol.

Why do we usually use ethanol 100% first then 70% in DNA

To make 70% alcohol from 95%, add to 70 c.c. of 95% alcohol enough water to make 95 c.c.. The result will be 95 c.c. of 70 % alcohol.. See "A simple method of diluting Alcoh The result will be 95...

There are two types of percent concentration: percent by mass and percent by volume. PERCENT BY MASS. Percent by mass (m/m) is the mass of solute divided by the total mass of the solution, multiplied by 100 …

Boiling Points HeartMagic- The music of Gary StadlerEthanol can be derived from sugar-based, corn-based, and cellulose-based materials. In Brazil, sugarcane is the feedstock of choice, while in the United States that designation goes to corn. The starch in corn kernels easily converts into simple sugars, with the enzyme catalyzing this process costing a mere .03-cents per gallon; the sugars are then fermented into alcohol (additives make it how to make bake and saltfish if you could make 100% pure alc it would be 200 prof so if you add 1/2 water by voc it would be 100 prof so to get 80 prof you would need about 45% alc by voc but you need to know for shure what prof alc it is to start with befor you start cut n it. How to receive dash from dash core

The water should be roughly 100 degrees Fahrenheit to ensure that all of the sugar melts. Fill the barrel halfway and pour in the sugar at 10 pound intervals until it is all used. This will make the solution easier to mix. Once the sugar is fully dissolved, you can fill up the barrel with hot water until it is nearly to the top. Once the mixture is at roughly 80 or 90 degrees, you can mix in

in many protocol for DNA extraction I have seen that 100% ethanol (absolute ethanol) is used first for DNA precipitation (washing) then 70% ethanol.

Distil it to remove the other substances. You may have to use fractional distillation, depending upon whether the other substances evaporate at a lower or higher temperature.

The water should be roughly 100 degrees Fahrenheit to ensure that all of the sugar melts. Fill the barrel halfway and pour in the sugar at 10 pound intervals until it is all used. This will make the solution easier to mix. Once the sugar is fully dissolved, you can fill up the barrel with hot water until it is nearly to the top. Once the mixture is at roughly 80 or 90 degrees, you can mix in

But 99 cubic centimetres of ethanol weigh 80 grammes not 99 grammes so the concentration of the solution expressed as w/w would be (1 x 100)/(1 + 80); i.e., 100/81=1.2% w/w. A further complication often comes with the symbols used for the quantities, volume being sometimes very confusing.