Primer software

Dear Sir!
I represent Hadassah Ein-Kerem Gene Therapy center, Hebrew University
Jerusalem.
We are presently looking for optimal PCR primer design software.
Our maing requirements for software are:
1. Possibility of on-line checking of primers uniqueness (not only
comparing to
template but also comparing to Human Genome). This requirement comes
from
necessity to make PCR to tissue samples.
2. We want to obtain several different options of primers (with
displacement
of several tens bases).
Thank you for the help!
Mark Katzenellenbogen.
Ph.D. student.
markatz at md2.huji.ac.il
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