The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adaptor molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and the dominant negative form of MyD88 attenuates IL-1R-mediated NF-kB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor, which are members of the Toll/IL-1R family of receptors.

Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adaptor protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. The MyD88 gene is expressed in many tissues.

Catalog #

M9755-10D

Applications

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution

Western Blot: 0.5-2ug/ml. A protein of approximate molecular weight of 32kD is detected.

Optimal dilutions to be determined by the researcher.

Positive Control

Whole cell lysate from human spleen or Raw cells

Storage and Stability

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Clone Type

Polyclonal

Isotype

IgG

Host

Rabbit

Source

Human

Concentration

~0.5mg/ml

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

Purity

Purified

Immunogen

Peptide corresponding to amino acid residues 233-248 of human MyD88.

Specificity

Recognizes human MyD88.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.