General

The new µ-PrepCell 2.0 (µPC 2.0) is specifically designed for highest robustness and ease of use. The entire cell is made of a fully inert polymeric material. The inlet block contains additional conductive carbon, to serve as Auxiliary (AUX) electrode. In addition the Reference electrode (REF) is positioned in a reference chamber, resulting in overall improved signal stability and reproducibility. In practice the new µPC 2.0 has distinguished advantages in both Oxidative and Reductive mode.

Compounds passing through the flow cell are instantaneously oxidized or reduced making the cell ideally suited for infusion EC/MS, HDX/MS or LC/EC/MS. The cell can be used in various application areas such as: drug metabolism (generation of metabolites), protein chemistry (reduction of disulfide bonds, H/D exchange, etc.), environmental degradation (generation of transformation products), stability testing of drugs, nutrients, etc. In continuous infusion mode over several hours, the cell can be employed for micro preparative work, generating µg quantities of REDOX products.

Like the ReactorCell™, the µPc 2.0 is based on a thin-layer flow cell concept, comprising of a very smooth working electrode surface over which the sample is flowing. This results in almost zero sample adsorption, unlike porous flow through cells which are prone to adsorption and carryover. The cell can be disassembled within a few seconds for easy cleaning or exchange of working electrode, e.g. making the µPC 2.0 the ideal workhorse. The cell is used with the ROXY Potentiostat or integrated in the ROXY EC or ROXY EC/LC system, respectively. Flow rates, potentials, events programming, recording of voltammograms etc., are fully controlled via the Dialogue™ Elite software.

Large Variety of Working Electrodes

For maximum selectivity, yield and wide applicability, a large variety of electrode materials are available such as Glassy Carbon (GC) and Magic Diamond™ (BDD: Boron Doped Diamond), Au and Pt. For reduction of Disulfide bonds in proteins/peptides the new TiBlue™ electrode is available. For more details, see specs

Oxidative Mode

Robust and reproducible oxidations• Superior selectivity

Superior selectivity

Large working range (potential)

Excellent correlation with metabolic & degradation pathway

The new conductive polymeric inlet block results in significant higher selectivity by suppressing unwanted reduction reactions when working under oxidative conditions (Ox only). The electrochemical oxidation of Amodiaquine was used as example. Using the new uPC 2.0, only the 3 oxidation products were formed without any of the reduction products.For more information, see brochure µ-PrepCell 2.0.

Another advantage is the larger potential working range as can be seen from the scanning voltammogram of Dopamine. With the new cell, a significant larger working range is possible. The previous cell showed a current overload at around +1.5 V, meanwhile the new cell showed no current overflow up to +2 V and much better reproducibility.

Reductive Mode

Superior robustness and selectivity

Works in simple DC mode (no more pulsing)

TiBlue electrodes for highest yield and ease of use

No longer polishing with disposable electrodes

In reductive mode the new µ-PrepCell 2.0 allows to work in simple DC mode for the cleavage of disulfide bonds in proteins/peptides. A potential of ca. -2.0 V is sufficient to reduce selectively the S-S bonds, making the use of square wave pulses obsolete. In combination with the new TiBlue™ electrode a much more stable and reproducible reduction is achieved. For more details, see brochure µ-PrepCell 2.0.

ROXY installation with µ-PrepCell in 2 minutes: click to play or download the movie (right click and "save link as..." if video does not start). If your player does not recognize the format, download the free player from VLC (Windows or Mac).