Measuredby the ability of the immobilized protein to support the adhesion of A431 humanepidermoid carcinoma cells. When 1 x 105 cells per well are added tormCol13a1 coated plate (2 μg/mL, 100 μL/well), approximately 45%-75% will adhereafter 1 hour at 37°C. Optimal dilutions should be determined by each laboratoryfor each application.

Endotoxin Level:

<1.0 EUper 1 μg of the protein by the LAL method.

Purity:

>80%,by SDS-PAGE under reducing conditions and visualized by silver stain.

Reconstitution:

Reconstituteat 300 μg/mL in PBS

Storage:

Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months,-20 to -70°C under sterile conditions after reconstitution.