CherryPicker Cell Capture & Separation Systems

Use the CherryPicker Systems to monitor, capture, and enrich a population of cells in which your promoter of interest is active or your protein of interest is being expressed. The cells of interest express a membrane-targeted red fluorescent protein, CherryPicker, that makes them easy to identify. Use the included magnetic beads and CherryPicker antibody to capture these cells in < 2 hr, and enrich the captured cells by re-culturing them.

Product

Comments

Systems for Promoter Studies (vectors included)

CherryPicker Cell Capture System, Cat. No. 632572

Contains the CherryPicker Reagent Kit, magnetic beads, a promoterless CherryPicker vector, and a control vector

Promoterless system suitable for use with most cell types. Use the CherryPicker Cell Capture System to capture and enrich cells in which your promoter of interest is active.

Promoterless system which can be used to transduce virtually any dividing or nondividing cells, including primary cells and stem cells. Use the lentiviral CherryPicker Cell Capture System to capture and enrich cells in which your promoter of interest is active.

Systems for Protein Studies (vectors included)

CherryPicker Cell Capture System (IRES), Cat. No. 632573

Contains the CherryPicker Reagent Kit, magnetic beads, a bicistronic CherryPicker vector, and a control vector

Bicistronic system suitable for use with most cell types. Use the CherryPicker Cell Capture System (IRES) to capture and enrich cells expressing your protein of interest.

Bicistronic system which can be used to transduce virtually any dividing or nondividing cells, including primary cells and stem cells. Use the CherryPicker Cell Capture System (IRES) to capture and enrich cells expressing your protein of interest.

Capture Only Those Cells in which Your Promoter of Interest is Active

Use the promoterless CherryPicker systems, CherryPicker Cell Capture System (Cat. No. 632572) and Lenti-X CherryPicker Cell Capture System (Cat. No. 632574), to monitor the activation of a promoter of interest, and capture those cells containing the active promoter. Insertion of a functional promoter into the promoterless reporter vector, pCherryPicker1, causes a red fluorescent protein (CherryPicker, a membrane-targeted version of the mCherry fluorescent protein) to be displayed on the surface of mammalian cells, which are then easily captured by a CherryPicker-specific antibody bound to IgG-coated magnetic beads.

Capture Only Those Cells Expressing Your Protein

Use the bicistronic CherryPicker systems, CherryPicker Cell Capture System (IRES) (Cat. No. 632573) and Lenti-X CherryPicker Cell Capture System (IRES) (Cat. No. 632575) to identify, monitor, and capture mammalian cells expressing your protein of interest. IRES technology results in simultaneous expression of a protein of interest and a membrane-targeted red fluorescent protein (CherryPicker) from the same transcript. Cells expressing your protein of interest must also express CherryPicker, a membrane-targeted version of the mCherry fluorescent protein, and can be captured on magnetic beads via a CherryPicker-specific antibody.

Coexpression of your protein of interest and CherryPicker allows you to easily:

Applications

Promoterless Systems:

Bicistronic Systems (IRES):

Monitor cells expressing a protein of interest

Capture and analyze cells expressing the protein of interest

Culture the captured cells as a more homogeneous population

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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