The lyophilized antibody is stable at room temperature for at least one month and for greater
than a year when kept at -800C.

Reconstitution:

When reconstituted in sterile water to a concentration
of 0.5 mg/ml the antibody is stable for at least six weeks at 2-40C.

Applications

ELISA:

In a sandwich ELISA (assuming 100μl/well),
a concentration of 1 μg/ml of this antibody will detect at least
0.5 ng/well of recombinant human BMP-7 when used with conjunction
with goat anti-mouse horseradish peroxidase (Jackson Laboratories).

Western Blot:

To detect h-BMP-7 by Western Blot analysis
this antibody can be used at a concentration of 2 µg/ml. Used
in conjunction with compatible secondary reagents the detection
limit for recombinant h-BMP-7 is 2.5 ng/lane.

Biological Activity:

To yield one-half maximal stimulation [ED50]
of the biological activity of hBMP-7 (50 ng/ml), a concentration
of 0.4 - 0.6 µg/ml of this antibody is required.