botulism

I would like to ask, wether you have any experience in cl. botulinum
toxin testing by methods, which could replace the mouse bioassay. I do
it by agargel-immunodiffusion with extracts of organs concentrated by
lyophilization, accepting the lack of sensitivity and a certain amount
of cross reactions, but avoiding these cruel mouse assays.
Dr. Henning Baehr, Govt. Vet. Laboratory Oldenburg, Germany