Since its broadcast debut on March 2, 2009, "Late Night with Jimmy Fallon" has created a huge online presence for itself with many of its popular segments, celebrity sketches and musical performances becoming viral hits. "Late Night" guests come to Fallon's studio 6B ready to play. Memorable moments include Fallon dressed as Neil Young in a parody of pop hit "Whip My Hair" with Bruce Springsteen, Justin Timberlake joining Fallon for a musical melody through the "History of Rap," and Fallon and Paul McCartney singing a duet of "Yesterday" with the original lyrics in a performance of "Scrambled Eggs."

Popular serialized "Late Night" segments include "Real Housewives of Late Night" and "Jersey Floor," which spoofs present day reality TV sensations. "Robert Is Bothered" and "Reflections of Justin Bieber" are other notable fan favorites, in addition to audience participation bits like "Models and Buckets," "Competitive Spit-Takes," and "Freestyling with The Roots."