Have you tried adding a little (1-2mls per 100) magnesium chloride (1M) to your
fixative.
Which fixative are you using, 4% PFA with 0.25% glut is quite good for immuno-EM
Janice Connolly
Brian S. Thoma wrote:
> All,
>> I've been trying to find the "best" formula for the preservation of CNS
> myelin for detection of myelin-associated proteins by immunocytochemistry. I
> have both epoxy (EPON) and acrylic (LR GOLD) resins available for use.
> If anyone is willing to share their experience I would greatly appreciate it.
>> Please respond by e-mail as well as by posting to the newsgroups.
>> Thanks,
>> Brian Thoma
>bthoma at lonestar.utsa.edu