Purpose :
To determine the effect of intravitreal administration of phosphate buffer saline (PBS) on retinal revascularization and neovascularization in the mouse oxygen-induced retinopathy (OIR) model. In addition, aflibercept (Eylea) treated eyes were used as reference control.

Methods :
C57BL/6 mice were exposed to 75% oxygen for 5 days and at P12 the mice were returned to normal room air. At P14 the mice were injected intraocularly with 0.5 μl aflibercept (Eylea, n=10) or PBS (n=9). Non-treated weight matched littermates (n=9 retinas) were also included in the study. At P17 retinal revascularization and neovascularization (pre-retinal tufts) were analyzed from Isolectin B4 stained retinal flat mounts using confocal microscopy and Adope Photoshop CC software.