Tet-tTS Transcriptional Silencer

pTet-tTS expresses the tetracycline/doxycycline-controlled transcription silencer (tTS), which is a fusion of the Tet Repressor Protein (TetR) with a KRAB silencing domain. tTS can be used to significantly lower the basal expression from 1st generation Tet-On Inducible Expression Systems. In the absence of doxycycline, tTS binds to the tet-operator sequences within TRE promoters, silencing gene expression. Upon addition of doxycycline, tTS undergoes a conformational changes that causes its release from the promoter, to be replaced by the Tet-On transactivator.

Features

Reduced basal expression when combined with 1st generation Tet-On System

Active suppression of transcription when bound to TRE promoters

Binds in the absence ofdoxycycline

Additional Information

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