“genetic distance and identification of two indian poultry breed by using dna fingerprinting”

Abstract

ABSTRACT:The RAPD method was used to generate fingerprint patterns for two breeds of chicken like Aseel and Brahma breeds. Specific results for each breed were obtained by RAPD method. RAPD-PCR pattern was useful to identify species composition of two breeds of fowl. RAPD fingerprinting of 30 samples of Aseel and Brahma chicken breedsamplified with 6 random primers.From the6random primers, a total of 24 bands were amplified and 17 of these ( about 66.9% ) were found to be polymorphic among the Aseel population and atotal of 28 bands were amplified out of which only 20 were polymorphic giving (about 70.95%) polymorphism in Brahma breed.The estimate of GI was highest (0.59) with the primer DF-02 and the lowest (0.12) with the primer DF-05. The genetic distance pooled over the primers was (1.10±0.05). The highest and lowest GD were 2.15 and 0.22 with (DF-05 and DF-04) respectively. The genetic identity pooled over the primers was (0.28±0.08) between these breeds and the MAPD estimate to be (58.63±0.20). It might be concluded that the genetic identity index between these two breeds on the basis of BF showed moderate level of GI with the primers. MAPD calculated on the basis of uncommon bands also demonstrated lower to medium level of genetic difference between Aseel and Brahma breeds of poultry.DNA Fingerprinting pattern displayed differences in position of bands, some of them appearing the characteristic of this study. Therefore, this study demonstrates the usefulness of RAPD Fingerprinting to distinguish within and between two strains of Chicken.