Adult SHB females will oviposit in cracks and crevices around the honey bee nest (Fig. 1, Lundie, 1940; Neumann and Härtel, 2004). Occasionally, the females will bite holes in the capping and/or side of brood cells, insert their ovipositor into the hole, and oviposit on the bee developing in the cell (Ellis et al., 2003a; 2003c; 2004d; Ellis and Delaplane, 2008). In response, adult worker honey bees can detect SHB eggs/young larvae present in capped brood cells, uncap the cell, and remove the brood and/or SHB eggs/larvae (termed hygienic behaviour). A number of methods have been developed to facilitate studying SHB oviposition behaviour and the resulting honey bee hygienic responses to the behaviour.