a All fresh samples were stored at room
temperature for 5 days, at which time DNA was extracted, and the DNA
extract was stored at 4°C. Never frozen DNA extract was maintained at
4°C before PCR, whereas the DNA extract was stored at −20°C for 1
week for experiments on frozen DNA extract.

b AmpliTaq Gold used for samples that did not
amplify by use of Taq polymerase.