Famous for appearing in the Dead of Alive fighting (DoA) game series as sister to Kasumi and Hayate, Ayane plays aide to the Hayabusa clan and Murai's Shadow Ninja clan throughout the Ninja Gaiden series.

Eventually she stops following Murai, instead following only Hayabusa's orders and helps him out with Kunai notes throughout the journey. She watches over him, though much younger than he at only 14 years of age at the time of Ninja Gaiden.

She makes another appearance in Ninja Gaiden II (Xbox 360) to return the Dragon's Eye to Ryu so he can return the Dragon Sword to power.

Ayane is a playable character in Ninja Gaiden Sigma 2 for PlayStation 3, where she has her own Story Mode and a moveset which seems to be taken directly from DoA.