We require 75x on-target depth (after reads are filtered for adapters, mapped, duplicates marked.), which is defined as on-target yield / target size. The raw throughput (raw data off the sequencer) may have about 130x-140x or higher.

We also have very high requirements on uniformity, e.g. very high percentage requirements on 1x and 20x loci. We will communicate the numbers individually for now. The uniformity requirements are higher than most of the research projects published.

We require samples have to match BOTH 75x and uniformity requirements.

Deliverable to consumers: VCF (with SNP and indels and no-calls (regions not covered by sequencing or not confident to call))

Deliverables to researchers: (if the researcher sets up a study and consumers contribute and consent to give their data): VCF, self-reported phenotypic data.