Increase in enzyme activity - (Jul/16/2014 )

It is usually a trend to see a decline in enzyme activity as time passes but I am seeing an increase in enzyme activity after a period of incubation/storage (after the partial purification). Are there any comments about this scenario?

-carollee-

since it's only partially purified there are a few possible reasons why enzyme activity may increase. here are a few which immediately come to mind:

partial hydrolysis of the enzyme (may deactivate regulatory subunit or portion of the enzyme or free the active site)