Figure 4.

Repression of PDC1 by Sir2 and Hst1 during the diauxic shift. (a) Overview of the glycolysis/fermentation pathway in yeast. Red lettering indicates
genes bound by Sir2, Hst1 and Sum1 across the ORF. Bold lettering indicates the genes
only bound by Sir2. (b) Quantitative RT-PCR analysis of PDC1 mRNA levels in log phase WT and mutant strains. Expression level in the WT strain
was normalized to 1.0. (c) PDC1 expression level in WT and deletion strains when progressing through the diauxic shift.
(d) Enlarged image of (c) showing the later time points. Error bars represent standard
deviation.