modal number = 46; range = 42 to 48.This is a pseudodiploid human cell line. The modal chromosome number is 46 occurring at 68% and polyploidy at 0.4%. Three marker chromosomes were detected in all metaphases examined. Two markers that appeared to be the product of the balanced translocation between N1 and N17. The third marker is ?inv(13). No other common markers were detected. Normal N1, N13, and N17 had single-copy each. Y chromosome is present.

The cells express receptors for bombesin at up to 6000 sites per cell.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: X,Y

CSF1PO: 11,13

D13S317: 8,11

D16S539: 10,11

D5S818: 12,13

D7S820: 9,11

THO1: 7

TPOX: 9,11

vWA: 16,18

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 2

PGM1, 1-2

PGM3, 1-2

Name of Depositor

V Larson

Permits

These permits may be required for shipping this product:

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.