Effect of the ionic environment on the incorporation of the intermediate-sized filament protein vimentin into residual cell structures upon treatment of Ehrlich ascites tumour cells with triton X-100. II. Ultrastructural analysis

Summary

Ehrlich ascites tumour cells were extracted in buffers containing Triton X-100 and mono-di- and polyvalent cations and then analysed by phase-contrast and electron microscopy. The results of this ultrastructural analysis confirm those of the biochemical analysis in the accompanying paper that the stability of intermediate-sized filaments is dependent on the ionic environment. Furthermore, the organization of filaments in long parallel arrays is dependent on the presence of divalent cations and can be inhibited, to some extent, by the presence of monovalent cations. The stability of other detergent-resistant structures, the boundary lamina, microfilaments, microtubules, centrioles, polyribosomes and the nuclear cortex, is also affected by the ionic environment but to a lesser extent.

Effect of the ionic environment on the incorporation of the intermediate-sized filament protein vimentin into residual cell structures upon treatment of Ehrlich ascites tumour cells with triton X-100. II. Ultrastructural analysis

Effect of the ionic environment on the incorporation of the intermediate-sized filament protein vimentin into residual cell structures upon treatment of Ehrlich ascites tumour cells with triton X-100. II. Ultrastructural analysis

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