Ligation buffer: contains oligonucleotides that hybridize to the PLA probes and all components, needed for ligation except
the ligase

Ligase (1 U/μl)

Amplification (5×) buffer: contains all components needed for Rolling Circle Amplification except the polymerase (oligonucleotide
probes labeled with a fluorophore that hybridizes to the RCA product are also included)