The determination of serum α- and β-lipoprotein cholesterol

Abstract

Abstract A method is presented for the estimation of serum lipoprotein cholesterol distribution suitable for routine studies. The technique involves filter paper electrophoresis, extraction of cholesterol from the fractions, followed by estimation using a modified ferric chloride procedure previously described. Recoveries appear to be quite satisfactory and reproducibility is of fairly high order. In addition some observations are made on the effect of deep frozen storage of the serum samples upon the analysis.

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