Conan O’Brien is back for a fifth year at San Diego Comic-Con, and on the first night (July 17) he brought a hilarious new cold open!

Conan debuted an animated cold-open parody of Spider-Man: Into the Spider-Verse. The clip parodies the beginning of the film, with the 56-year-old late-night host starring in the central superhero role.

“Alright, let’s do this one last time,” Conan O’Brien says in voiceover, as a magazine cover is shown onscreen with the title Conan O’Brien: My Battle with Eczema. “My name is Conan Denise O’Brien. When I was 15 years old, I was bitten by a radioactive party clown at my Quinceanera. You probably know the rest.”

“I could have used my powers to fight crime, battle evil and rescue innocent people, but instead I opted for cheap laughs and light celebrity chat,” Conan continued – Watch the hilarious segment below!