Application Information: CDC2 antibody [3E1.1]

WB: Use at an assay dependent dilution. Predicted molecular weight: 34 kDa. Optimal dilutions/concentrations should be determined by the end user.

Concentration

1
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

IgG fraction

Specifications: CDC2 antibody [3E1.1]

Full Name

cyclin-dependent kinase 1

Background

The cell division control protein (cdc2) kinase is a catalytic subunit of a protein kinase complex, called the M phase promoting factor, that induces entry into mitosis and is universal among eukaryotes. In the fission yeast Schizosaccharomyces pombe, the gene Cdc2 is responsible for controlling the transition from G1 phase to the S phase and from the G2 phase to the M phase of the cell cycle. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex that is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase.

Synonyms

cdc2-prov, CDC2, 380246, MGC53829, cdc2prov, cdc2 prov

Cellular Localization

Nuclear

Specificity

3E1.1 reacts strongly with immunoblotted antigen but does not immunoprecipitate native p34cdc2.

Host

Mouse

Clonality

Monoclonal

Clone Name

3E1.1

Isotype

IgG1

Immunogen

C terminal fragment, starting from methionine 85 of Xenopus Cdc2 .

Antigen Species

Xenopus laevis

Species Reactivity

Xenopus laevis

Conjugation

Unconjugated

Storage Conditions: CDC2 antibody [3E1.1]

Storage Buffer

Preservative: None. Constituents: PBS

Storage Instruction

Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Notes

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.