Our objective was to investigate the potential utility of polychlorinated biphenyl (PCB) residues in fish tissue as an indicator of relative motility of fish species in Lake Logan Martin, a 6,179 ha reservoir located in east-central Alabama. Choccolocco Creek, a mid-lake tributary, was the site of deposition of PCBs and this embayment had the highest concentrations of PCBs. The relationship between PCB concentrations in fish muscle tissue and distance of the fish, at capture, from the Choccolocco Creek embayment was used to infer relative motility of 12 species of warmwater sport and commercial fishes. Regression analyses of PCB concentration versus distance suggested that species ranged from low motility (highly significant regression and relatively high R2) to highly motile (insignificant regression or relatively low R2 ). Centrarchids were relatively immotile, moronids were relatively motile and flathead catfish Pylodictis olivaris, exhibited greatest motility. Our results reflect long term (perhaps lifetime) movements of fishes in one reservoir and provide motility information on spotted bass Micropterus punctulatus, blue catfish Ictalurus furcatus, freshwater drum Aplodinotus grunniens, and white bass Morone chrysops, for which little has been reported. Where PCBs occur in relatively discrete areas of aquatic ecosystems, this method can provide a long-term perspective on movements of numerous fish species under similar environmental conditions.