Figure 3. Morphology of hESCs in a feeder-free culture.
BG01V cells were cultured under feeder-free conditions, using KnockOut™ SR XenoFree directly with KnockOut™ SR GF Cocktail on CELLstart™ Matrix in Knockout™ DMEM. At passage 15, BG01V cells were cryopreserved in the same medium and placed in liquid nitrogen storage. Photos show attachment and growth of BG01V when cryopreserved cells were recovered in feeder-free KnockOut™ SR XenoFree Medium, from day 1 (A) through day 3 (B) post thaw.

Components required for Feeder

Free expansion of ESCs and iPSCs in serum-free and xeno-free culture system