[en] In this paper, a simple and rapid method was developed in order to assess in
comparative tests the production of binary biogas mixtures containing CO2 and another
gaseous compound such as hydrogen or methane. This method was validated and
experimented for the characterisation of the biochemical hydrogen potential of different
pure strains and mixed cultures of hydrogen-producing bacteria (HPB) growing on glucose.
The experimental results compared the hydrogen production yield of 19 different pure
strains and sludges : facultative and strict anaerobic HPB strains along with anaerobic digester sludges thermally pre-treated or not. Significant yields variations were
recorded even between different strains of the same species by i.e. about 20% for three
Clostridium butyricum strains. The pure Clostridium butyricum and pasteurianum
strains achieved the highest yields i.e. up to 1,36 mol H2/mol glucose compared to the
yields achieved by the sludges and the tested Escherichia and Citrobacter strains.