quote:
"For agarose gel electrophoresis, 50x TAE should be diluted to a working concentration of 1x. The TAE buffer should be
replaced after each electrophoresis run due to the anode solution becoming alkaline and the cathode solution acidic, which
results in lowered DNA mobility."

"For agarose gel electrophoresis, 50x TAE should be diluted to a working concentration of 1x. The TAE buffer should bereplaced after each electrophoresis run due to the anode solution becoming alkaline and the cathode solution acidic, whichresults in lowered DNA mobility."

It is OK for us to re-use 0.5X TAE for more than 10 times in our lab. But we don't keep the gel in the gel box after running gel each time and take the gel out, cut the dye band out. The rest part you can reuse.

hi
in our lab we re-use the home made buffer and the gells as much as we can for routine work. in fact i pour my gells on glass plates to make them thin and re-use it unless it is not broken in handling.
chandima

hi
in our lab we use the buffer as far as 20 times or more. The buffer is changed when the colour has changed to blue due to the bromophenol blue used in loading buffer. But don't forget to remove ethidium bromide of the buffer (using columns from schleicher and schuell or activate carbon)

I generally reuse gels until I run out of lanes - however, if they do go off, it isn't the resolution that goes, but for some reason the samples don't sink into the lanes as well after a while. Not sure if that's just me though!

so how do you reuse a gel? do you just run your samples off the bottom and then reload new ones? i take it you dont add ethidium bromide to the actual gel itself before it sets? how do you get rid of the ethidium anyway - whether you add it to the gel or whether you stain your gel afterwards?

i figured eppendorf just wants you to buy more buffer, but i make my own anyway - someone just told me once that you cant reuse it! evidently, they were very wrong!