Electric switch could turn on limb regeneration

Tadpoles use a proton pump to direct tissue regrowth.

Heidi Ledford

Tadpoles: chop off their tails and they grow back.

NHPA

Tadpoles can achieve something that humans may only dream of: pull off a tadpole's thick tail or a tiny developing leg, and it'll grow right back — spinal cord, muscles, blood vessels and all. Now researchers have discovered the key regulator of the electrical signal that convinces Xenopus pollywogs to regenerate amputated tails. The results, reported this week in Development, give some researchers hope for new approaches to stimulating tissue regeneration in humans1.

Researchers have known for decades that an electrical current is created at the site of regenerating limbs. Furthermore, applying an external current speeds up the regeneration process, and drugs that block the current prevent regeneration. The electrical signals help to tell cells what type to grow into, how fast to grow, and where to position themselves in the new limb.

To investigate, Michael Levin and his colleagues at the Forsyth Center for Regenerative and Developmental Biology in Boston, Massachusetts, sorted through libraries of drug compounds to find ones that prevent tail regeneration but do not interfere with wound healing. One such drug, they found, blocks a molecular pump that transports protons across cell membranes; this kind of proton flow creates a current.

Levin speculates that the current generated by this proton pump produces a long-range electric field that helps to direct what happens to nerve cells pouring into the site. "We can use this hydrogen pumping as a top-level master control to initiate the regeneration response," says Levin. "We didn't have to specifically say, 'put a little muscle over here, a little muscle over there'."

The proton pump could also be used to turn on limb regeneration in older tadpoles that would normally have lost this ability. When Levin and his colleagues activated the proton pump during this older phase, tadpoles were more than four times more likely to regrow a perfectly formed tail than their normal counterparts.

Chop and change

The notion of regenerating complex organs from adult cells hasn't always been popular, says David Stocum, director of the Indiana University Center for Regenerative Biology and Medicine in Indianapolis. "People used to pooh-pooh the idea," says Stocum, "but now there's renewed interest in it." That interest has been primarily focused on the regenerative power of stem cells. But there is also some interest in direct regeneration from adult cells at the wound site.

Many children under the age of seven have regrown amputated fingertips.

At first glance, dramatic limb and tail regenerations seem to be restricted to 'simpler' creatures, such as the humble planaria flatworm — chop it up into a hundred pieces and you'll soon have a hundred little worms on your hands — and salamanders, which can grow back limbs, tails, jaws, intestines and some parts of their eyes and hearts.

But there are impressive examples of tissue regeneration in mammals as well. Male deer can grow the bone, skin, nerves and blood vessels of their antlers at a millimetre a day. Humans can regenerate livers, and many children under the age of seven have regrown amputated fingertips. And then there are the odd medical journal case studies of patients who have lost, say, a kidney, only to find years later that they've sprouted a new one.

Simple switch

Changes in electrical current have been measured in regenerating fingertips, just as in a tadpole's regenerating tail. But converting humans into fully functioning regenerators will probably take more than directing bioelectrical signals. The formation of scar tissue, for example, could inhibit regeneration in some cases, says David Gardiner, a biologist at the University of California, Irvine.

But the complex networks needed to construct a complicated organ or appendage are already genetically encoded in all of our cells — we needed them to develop those organs in the first place. "The question is: how do you turn them back on?" Levin says. "When you know the language that these cells use to tell each other what to do, you're a short step away from getting them to do that after an injury."

The simplicity of the regeneration start signal is promising, says Stocum: it is just possible that a properly tuned electric signal is all humans need to jumpstart tissue regeneration.

Let me show you one project in particular. She led them to a bright, cheerful ward where a rather young Lady was reclining with her left arm embedded in what looked like an electronic fish aquarium.

Hi, Linda. Id like you to meet Barbara Reigher, and Kayla Hawkes. Kayla is the Food Services Director here at the Hospital, shes the one to go to for your favorite food.

Linda smiled at them, I dont really have a favorite food, but ever since my accident Ive been wanting to eat corn-on-the-cob, I think because its the only food you need two hands for.

Dr. Sanchez continued, Linda lost her left hand in a farming accident a year ago. We are attempting to grow her a new one.

Barbara was jolted, Thats impossible!, she blurted out.

Dr. Sanchez smiled, Thats what makes it a challenge! But truthfully, whats impossible about it? We know how to grow skin, and blood vessels, and bone. Thats what is going on inside this biotank. A matrix of nutrients, cell tissues, and enzymes are orchestrating the growth of a hand-shaped organ."

"Microsurgery teams are making attachments to the ligaments and blood vessels in the lower arm. The most challenging aspect, mechanically, is the joint structure, which is incredibly complicated in biological form. And we still havent mastered nerve regeneration. We plan to bypass that difficulty by using optical channels to an interface with the spinal chord."

"With embedded thermocouples, muscle actuators, and other sensors, Linda should have near-normal function with her new hand, and fortunately, shes right-handed, so she should enjoy a normal life.

3
posted on 02/28/2007 8:26:21 PM PST
by NicknamedBob
(You may not grok eating the sandwich, but the sandwich groks being eaten.)

If...there were an Intelligent Design to the genetic code...then this regrowth could be possible if we find how to open the valve that replenishes the basic building materials.

Chuck Missler of www.KHouse.org talked about some of this a while back in the light of the movie Jurassic Park and how the building blocks that constitute us--are generic, whereas the information in the DNA is like the software that runs the hardware.

Google up "Low intensity pulsed ultrasound". Fascinating stuff. While LIPUS is being used to regrow teeth and has implications for bone fracture healing, regrowing an entire arm would be pretty darn cool.

Considering the shape my knees are in? I could possibly be worth it to chop off both legs and let 'em regrow with new knees...

19
posted on 03/01/2007 1:16:40 PM PST
by Dead Corpse
(Anyone who needs to be persuaded to be free, doesn't deserve to be.)

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