I am interested in a gene which is null lethal but I need to temporary induce diminished capacity. If a cell is homozygous is it possible to induce heterozygous phenotypes or a partial knockout from WT with siRNA or other molecular methods without virus vectors? I'm not interested in a medillian solution but an inducible molecular way.
Thank you

It would help if you gave more information about the system that you are working with - what type of cells?
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Alan BoydNov 2 '12 at 7:04

@AlanBoyd im interested in mice and humans and i added the gene of interest as well.
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user1357Nov 2 '12 at 7:16

The tetracycline-regulated systems developed by Clontech would allow you to do something like this - the level of gene expression can be titrated by varying the concentration of tetracycline/doxycycline in the growth medium. See clontech.com/GB/Products/Inducible_Systems/… for links to more details - the early generation products might be a good place to start reading.
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Alan BoydNov 2 '12 at 18:23

@AlanBoyd my growth\life medium would prohibit the use of tetracycline but thanks for using the key word titrated
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user1357Nov 3 '12 at 0:10