* The [[AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media|Expression Culture]] and [[AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media|Starter Culture]] procedures were followed with the same modifications as on [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/18|9/18]]. These cultures are to be used to grow more <i>E. Coli</i>, this time with a plasmid coding for mutated Adenosine Deaminase (ADA)

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* Samples of Au/BSA made [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|last week]] were analyzed by Atomic Absorbtion Spectroscopy.

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**Before they were analyzed, solutions were centrifuged at 3000 rpm at 10°C for 5 min and fluid was separated from fibers. This is because the fibers would clog the instrument's tubing.

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**Between samples, water was run in order to clear residue.

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==Data==

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[[Image:AAgold2.jpg]]

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==Discussion==

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*Our AA spectra is very similar to the one obtained [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/04|9/4]] for 60-170 [Au/BSA]. Both show a drop to near-zero at 128, after an increase. This time the value at 100 is near-zero, which defies the trend and the previous results and may possibly be due to experimental error.

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Objectives

Starter and Expression Culturess

The Expression Culture and Starter Culture procedures were followed with the same modifications as on 9/18. These cultures are to be used to grow more E. Coli, this time with a plasmid coding for mutated Adenosine Deaminase (ADA)

Samples of Au/BSA made last week were analyzed by Atomic Absorbtion Spectroscopy.

Before they were analyzed, solutions were centrifuged at 3000 rpm at 10°C for 5 min and fluid was separated from fibers. This is because the fibers would clog the instrument's tubing.

Between samples, water was run in order to clear residue.

Data

Discussion

Our AA spectra is very similar to the one obtained 9/4 for 60-170 [Au/BSA]. Both show a drop to near-zero at 128, after an increase. This time the value at 100 is near-zero, which defies the trend and the previous results and may possibly be due to experimental error.