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Through many different initiatives, the theatrical release of 3 Needles raised over $50,000 for AIDS charities. The film opened in several major cities for a special limited engagement on December 1, 2006, in conjunction with World AIDS Day. Proceeds from opening weekend screenings benefited local and national AIDS charities, including NYC's Gay Men's Health Crisis, the Boston Living Center, and the AIDS Healthcare Foundation in Los Angeles, among others. 3 Needles was also elected as the Opening Night Film of the 2006 United Nations AIDS Film Festival, which presented Fitzgerald with the prestigious Berthe Meka Award. Supporters of 3 Needles rallied around the film's MySpace profile, gathering over 5,000 friends to raise funds for the Nova Scotia Gambia Association, a non-profit supporting AIDS relief and education in war-ravaged Sierra Leone.

The DVD edition of 3 Needles will include a wide array of informative and fascinating bonus features, including behind-the-scenes interviews with actors Lucy Liu, Shawn Ashmore, and Chloë Sevigny; trailers; photo gallery; deleted scenes; two short documentaries; and more. The two short documentaries are "China AIDS Initiative with Magic Johnson and Yao Ming" and "House on Fire—AIDS in America."

In China, Ping (Lucy Liu) is a pregnant young woman running a black market blood collection scam that creates a mini-epidemic in a rural village. In Montreal, Denys (Shawn Ashmore) is a porn actor hiding his positive HIV status in order to continue working and supporting his mother (Stockard Channing), who herself goes to extreme lengths to provide for the family's future. And, in Africa, Sister Clara (Chloë Sevigny) is a young novice nun driven to convert the rapidly dying Africans to Catholicism and makes a desperate bargain with a corrupt plantation owner to help prevent the spread of HIV in the region. Sandra Oh and Olympia Dukakis portray nuns in the same order who accompany her on the journey.