Associated products

Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

ELISA

1/100 - 1/1000.

IHC-P

1/100.

ICC/IF

1/200. PubMed: 19359471

Target

FunctionRequired for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.

Anti-Anterior Gradient 2 antibody images

ab22208 staining Anterior Gradient 2 in the NIH3T3 cell line from Mouse fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal(1/500) was used as the secondary antibody.

ab22208 staining Anterior Gradient 2 in the COS1 fibroblast cell line from Monkey kidney by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 16 hour at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary antibody.

Thank you for your rapid reply. I understand that you are concerned about the quality of ab30927 and take your comments very seriously. Please be assured that should we suspect that the quality of one of our products is not of the highest standards we ...

I'm sorry to hear you are having a problem with ab22208. As noted on the datasheet, this antibody has not been tested for Western blotting, only ELISA and IHC. However, the following protocol modifications may improve your results:
1. Fully denature...