E. coli are grown in SOB medium (supplied with Cat. # GZ-5), washed and suspended in our supplied Competent Buffer. The bacterial cells are now ready for transformations. The transformation efficiency is 2x108-1x109 transformants per μg of pUC19 plasmid. The efficiency of transformation varies depending on E. coli strain and plasmid size.

The competent cells can be used immediately or stored for later use. The kit is supplied with reagents sufficient for processing up to 300 x 100μl aliquots of competent cells for transformations (Cat. # GZ-4) or supplied with reagents and SOB culture media sufficient for processing up to 100 x 100μl aliquots of competent cells (Cat. # GZ-5).