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PowerPoint Presentation:

To evaluate the safety of updated GonaCon TM formula in Mexico. To evaluate progesterone levels and the titers of antibodies against gonadotropin releasing hormone ( GnRH ). To document any effects of toxicity in domestic female dogs in Mexico. To see that simultaneous applications of GonaCon TM and the parenteral rabies vaccination used in Mexico does not limit the immune response to both treatments. To evaluate the risk of possible local adverse reactions on the application area of updated GonaCon TM formula. OBJECTIVES The number and kind of adverse local reactions is less and in a minor intensity that the reported in previous studies. The new GonaCon TM reduces the progesterone levels and develop antibodies titers against gonadotropin releasing hormone ( GnRH ). GonaCon TM has less adverse effects of toxicity in domestic female dogs in Mexico compared with previous versions. The simultaneous applications of GonaCon TM and the parenteral rabies vaccination in Female dogs in Mexico do not limit the immune response for both products. The updated GonaCon TM formula is not generate local adverse reactions that could suggest discontinuing its use in Mexico . HYPOTHESIS GonaCon TM Study in Mexico

PowerPoint Presentation:

PowerPoint Presentation:

Progesterone At Day 0, THR levels in Groups A and B were lower than Group C (F=1.64, P=0.222). At Day 31 THR levels in Groups B and C were significantly lower than Group A (F=1.11, P=0.0351), which did not receive GonaCon ™. At Day 61, THR levels in Groups B and C were equal and lower than Group A (F=2.22, P=0.137 ). GonaCon TM Study in Mexico Statistical Findings Rabies VNA titers All dogs were reported to have been rabies vaccinated; dogs in Group B were not revaccinated against rabies. At Day 0, rabies VNA titers in Groups A and C were similar, but lower than Group B (F=2.46, P=0.116). At Day 31, rabies VNA titers in Group B were lower than Group A. Also, Group C titers were lower than Group B (F=3.01, P=0.076). At Day 61, rabies VNA titers in Groups B and C were lower than Group A (F=3.92, P=0.040) . Anti- GnRH antibody titers GonaCon TM was never used in these dogs before this study. At Day 0, no anti- GnRH immune response was detected. At Day 31, anti- GnRH titers among dogs in Groups B and C were similar, but higher than Group A (F=18.19, P=0.0001). The same results were observed on Day 61 (F=10.27, P=0.001) .

PowerPoint Presentation:

PowerPoint Presentation:

Less and minor intensity of adverse local reactions than in previous formulation used. GonaCon TM reduces progesterone levels and develop antibodies titers against GnRH . GonaCon TM had not generated adverse effects of toxicity. Simultaneous application of GonaCon TM and rabies vaccine did not limited the immune response. GonaCon TM Study in Mexico Conclusions and Recommendations

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GonaCon TM generated NOT measured muscle atrophy. Observed chronic granulomatose myositis and the diffuse coagulative necrosis were not limping source. No dejection, ulceration or paralysis were observed. A new study in owned female dogs during a long period (2 years) could be useful to measure the real possibilities to use the product in Mexico . GonaCon TM Study in Mexico Conclusions and Recommendations

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All dogs were clinically evaluated and reported in several stages of metabolic alterations (hepatic and renal function). It looks like these metabolic problems are not related with the study but is needed more analysis about this condition . A full report of macro and microscopic findings in the collected organs and tissues will be useful. All animals into the study had previously received rabies vaccine. We have no chances to get non vaccinated dogs in the field. GonaCon TM Study in Mexico Special Considerations – Lessions Learned

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The FAVN test were adjusted for the top of 13.8 UI/ml. It is needed to compare FAVN and RIFFT titers to better understand rabies antibodies responses. On the last days was detected the muscle atrophy. We had not a previously established strategy to make systematic measurements, but observations were recorded. During the study 2 female dogs into the Group B, and 1 into the Group C, that were pregnant and had puppies in that time are kept alive and they are clinically healthy. GonaCon TM Study in Mexico Special Considerations – Lessions Learned