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Anti Granulocyte Macrophage colony-stimulating factor antibody

Background

Granulocyte/Macrophage colony-stimulating factor (GM-CSF) is a hematopoietic growth factor that
stimulates the development of neutrophils and macrophages and induces the proliferation and
development of erythroid, megakaryocyte and eosinophil progenitors. GM-CSF is produced by
endothelial cells, fibroblasts, activated T cells, NK cells and macrophages. The antibody reacts with
human GM-CSF and can neutralize the bioactivity of natural or recombinant GM-CSF.

Immunogen and Recombinant Production Host

This antibody was generated from a healthy individual by a method based on Epstein-Barr virus
transformation of peripheral blood mononuclear cells followed by the isolation of antibody-producing
cells. The antibody reactivity for the target antigen was screened by enzyme-linked immunosorbent
assay (ELISA) using recombinant hGM-CSF. The antibody genes were cloned from the
antibody-producing cells and introduced into CHO-K1 cells for antibody production.

Application Note

Recommended Starting Dilutions:

For ELISA: Use at 1:500 – 1:4000

Not yet tested in other applications.

The optimal working dilution should be determined experimentally by the end user.