Cell Line Protocols

These protocols include directions for preparing medium and maintaining the cell lines, along with a few protocols for experimental procedures using cell lines. We expect to add additional protocols as we find time to do so. In addition, users who are not experienced with Drosophila cell culture may find it useful to read on of these reviews.

Gelutol - Preparation of gelutol mounting medium for use in the immunostaining protocol.

Creating new embryonic cell lines - Detailed description of the procedure used in the Miyake laboratory for generating embryonic cell lines in the 1980s. This protocol was written with the help of Ryu Ueda and Kumiko Ui-Tei, who made many of the cell lines in our collection when they were post-docs in the Miyake laboratory.

Transferring cells using trypsinization - Trypsinization procedure that we recommend for a few strongly surface-adherent cell lines. Most of our cell lines can be transferred easily without trypsin treatment.

Citing the DGRC

When publishing experiments using materials obtained from the DGRC
please cite the Drosophila Genomics Resource Center, supported by NIH
grant 2P40OD010949, in the acknowledgments. Your cooperation helps us
when we need to renew our grant.