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Carbon carbon everywhere...

A while ago I wrote about the Great Oxidation Event, the point way back in the history of the earth where a lot of little blobby organisms suddenly discovered the trick of using sunlight as energy, and in the process producing a reaction that liberated oxygen (photosynthesis). This (as I discussed in the previous post) had a major impact on all the rest of the life on earth, but it also rather majorly effected the photosynthesising organisms themselves, not so much because of the increase in oxygen, but because of the semi-simultaneous decrease in carbon dioxide.

When photosynthetic organisms first developed, there was a lot more carbon dioxide in the environment for them to use (with the world so new and all...) and therefore they weren't particularly bothered about getting hold of it. However when the carbon dioxide levels dropped (along with a potential rise in temperature) they were suddenly in very real danger of suffocating. These were marine organisms, and there just isn't that much carbon dioxide in seawater. There's plenty of carbon floating around, certainly, but it's all in bicarbonate form (HCO3- rather than CO2) and Rubisco, the main enzyme involved in photosynthesis, doesn't know how to use bicarbonate, it relies exclusively on carbon dioxide.

Our lecturer called it the 'Ancient Mariner paradox'; the ocean is full of carbon, but the photosynthesis machinery just couldn't use it:

"Water, water, everywhere,

And all the boards did shrink;

Water, water, everywhere,

Nor any drop to drink."

This left the little suffocating blobs with three options. They could stay remain tiny (as the picoplankton did) to minimise diffusion differences and therefore still survive dispite the low carbon dioxide levels. Or they could try to change the way Rubisco worked, but Rubisco has a rather compromised active site as it is, having to both distinguish between carbon dioxide and oxygen and trying to keep carbon dioxide processing levels high. Rubisco is often criticised as being an 'inefficient' enzyme, and compared to other enzymes it is, but with carbon dioxide levels at the level they are in the sea it's only ever working at half of its maximum speed. Carbon dioxide is the clear limiting factor.

So instead, these photosynthetic organisms started to develop ways to get carbon dioxide into the cell and concentrating it around the Rubisco. The main factor in this was the enzyme carbonic anhydrase, which converts bicarbonates back into carbon. However doing that inside the cell just leads to the carbon dioxide diffusing right back out again and therefore today almost all photosynthetic bacteria (and chloroplasts inside plants) contain a special internal compartment, a protein coat surrounding the Rubisco, and all the carefully hoarded carbon dioxide:

Figure above shows TEM of bacteria with carboxysomes pointed out by arrows. The scale bar on the bottom right is 100nm

Photosynthesising bacteria (apart from the picoplankton) contain a compartment called a carboxysome, which consists of a protein coat which contains carbonic anhydrase enzyme and Rubisco, allowing carbon dioxide to be produced right where it's most needed. The addition of a number of bicarbonate transporters on the outside of the cell allows bicarbonate to be brought into the cell, and the whole assembly is known as a Carbon Concentrating Mechanism, or CCM.

When these photosynthesising proto-bacteria were then picked up by free-moving proto-algae to become chloroplasts, they kept their CCMs. The CCM of eukaryotic chloroplasts is called a pyraniod, and can be seen in the picture below (from Dartmouth College) as the dark black blob in the upper left hand corner. The white things that it's surrounded by are starch grains. The big fuzzy blob below it is the cell nucleus, and the little grey membrane-filled circles are the mitochondria. The long black threads are either thylakoid membranes (inside the chloroplast) or endoplasmic reticulum:

The first algae would have been marine as well, and would have needed the CCMs in their chloroplasts in order to produce energy. Sea water tends to be alkaline, which means that the biocarbonate: carbon dioxide ration is insanely large. Gasses don't diffuse very well in water either, carbon dioxide takes about about 10 000 times longer to get anywhere in liquid compared to air.

In fact the best thing to do to get as much free carbon dioxide as possible is to leave the water altogether, and head out onto the land. But that is different story.

4 comments:

I remember hearing that the first major extinction was caused by oxygen poisoning. Just like we're worried about too MUCH carbon dioxide in the air, back in way back when having too little carbon dioxide was the major concern. They should have been having summits or something.

Really nice description of the enzymatic challenges of photosynthesis, and a nice protein and cellular view of autotroph evolution. Does this paper suggest that dropping CO2 levels were a selective pressure for the evolution of autotrophic eukaryotes? Does the CCM work more efficiently when further shielded within its new eukaryotic home?

@Skellett: Thanks for all your comments! Lol given the successfulness of our summits, whose to say they didn't! And yeah, oxygen poisoning wiped out a *lot* of little proto-bacteria, and probably some fascinating internal biochemistry.

@Mason: The paper didn't actually mention land plants at all, I just put that in as a suggestion made by our lecturer. I think that generally the CCM works *less* effectively when surrounded by eukaryotic cells, which is why there would have been such high selection pressure for the euks to get out of the water where you don't need the CCM so much as there's a lot more free CO2 which can diffuse more easily.

Getting out onto the land probably creates as big a problem as it solves, though. Oxygen is less soluble in water than CO2, so there's very little of it even in well oxygenated water. Out here, it's at 21%!

Do plant chloroplasts still have cyanobacterial CCMs? I was under the impression that they didn't. Certainly some plants have gone to great lengths to create their own CCMs.