In the human body the gastrointestinal (GI) tract is lined with mucosal tissues or mucosa. As shown in Figure 7.0.1, mucosa is primarily comprised of epithelial cells that are attached to the basement membrane. Protruding from the wall of the intestine are tiny, finger-like projections called villi that increase the absorptive area and the surface area of the intestinal wall. Digested nutrients (including sugars and amino acids) pass into the villi through diffusion. Circulating blood then carries these nutrients away. Unlike the mucosal tissue of the inner surface of the eyelids or the mouth, the epithelial cells which line the inside of the stomach are exposed to much harsher conditions, e.g., acid (i.e., hydrochloric acid), sometimes alcohol, enzymes (e.g., pepsin) for digesting food and waste generated therefrom. Mucous secretion essentially protects the cells on the inside of the stomach and duodenum from damage by acid or enzymes, for example by presenting bicarbonate to neutralize some of the effects of acid on the stomach's inner lining, as well as inhibitors to block the enzymatic activity. Once the mucous secretions of the epithelial cells stop, the inner lining of the stomach or duodenum would eventually be eroded by the combined action of acid and enzymes, leading to ulcer.

Normal human living intestine was obtained from surgical operations, and enzymatically digested so as to obtain dispersed single cells from the intestine samples. The suspended intestinal cells were cultured in MEM medium and divided into two groups: test group and control group. The MEBO regenerative nutrients were added to the cultures in the test group, while the control group did not receive any supplement. Every 3 days the old growth medium was discarded and fresh growth medium same as the first round was added to the cultures in the respective groups.

After 55 days of culture, in the treatment group the cells had grown into groups of cells appearing in various forms, including proliferation and differentiation of the cells isolated from human intestine samples. As shown in Figure 7.4.1.1a-c, in some areas, some of cells formed clusters that appeared to look like pieces of tissue. Surprisingly, it was observed that in some areas, tissue pieces resembling a fully assembled villus structure appeared in the culture Figure 7.4.1.1d. In comparison, most cells in the control group had died by Day 55.

To verify whether intestinal villus can be formed from proliferation and differentiation of isolated dispersed intestinal cells in vitro in the presence of the MEBO regenerative nutrients, experiments were conducted using intestines isolated from a mouse fetus (Figure 7.4.1.2a-c). The mouse fetal intestine was cut into small explants and cultured in cell culture medium in the presence or absence (control) of the MEBO regenerative nutrients (Figure 7.4.1.2d-f). Consistent with observation of cells from human intestines, single cells migrated from the explants started to form clones (Figure 7.4.1.2h-i) in the culture which grew to form tissue pieces having the same structure of a villus were formed in the presence of the MEBO regenerative nutrients (Figure 7.4.1.2j-m). In comparison, intestine explants in the control culture disintegrated (Figure 7.4.1.2g). A cross-sectional view (Figure 7.4.1.2n, p) of the villus shows that the tissue formed in vitro in the presence of the MEBO nutrients has the same structure as that obtained from tissue sections of intestines of a mouse fetus (Figure 7.4.1.2o) as shown in Yang et al. (2001) “Requirement of Math1 for secretory cell lineage commitment in the mouse intestine” Science 294:2155-2158.

These results are groundbreaking as they demonstrate that the MEBO regenerative nutrients can sustain vigorous proliferation and facilitate orderly differentiation of somatic cells for a long period of time to form multiple cell types that are linked together to form tissue with physiologically correct structure in the culture. From single, isolated cells from human and mouse intestines at least 4 types of cells are formed: entercytes, mucus-secreting goblet cells, enteroendocrine cells, and Paneth cells which are originated from epithelial stem cells in the crypts of the intestine. Although it remains to be ascertained the nature of the single, isolated cells that produced the multiple cells types in the culture containing the MEBO nutrients, it is significant that physiologically correct tissue has been cloned in vitro from single cells isolated from intestines. To our best knowledge, there is no report from others with the same results.

Brief introduction of MEBO in GI regeneration

Gastrointestinal regeneration and restoration technique is a technique nourishing and promoting somatic cells which have potential regenerative ability existing in the gastrointestinal mucosa induced into stem cells, and then in situ regenerate new mucosa tissue, and in situ replace aged mucosa; further prevent gastrointestinal mucosa damages, and recover the structure and function of the gastrointestinal mucosa. In this way, this technique can ensure the digestion and absorption of nutrition to supply adequate nutrition for the cells in all tissues and organs of human body, prevent primary diseases and retard tissue aging. Meanwhile, personal biotic clock diet plan is enacted according to personal condition of gastrointestinal biotic clock to maintain the physiological condition of the gastrointestinal mucosa all along.

Gastrointestinal regeneration and restoration technique is realized through the collective effect of cell regeneration gastrointestinal mucosa in situ technique, MEBO Gastrointestinal Capsule as well as the biotic clock diet plan.

3.1 Scientific proof of the protection function of MEBO GIC for mucosa

The protection state of GIC on the mucosa surface

3.2 Scientific proof of the protection function of MEBO GIC for mucosa

Animal experiment showing GIC can prevent alcohol damage

The content of MEBO GIC sticks on the gastric mucosa surface, which can strengthen the protection function of mucosa mucus, prevent the stimulus of alcohol, spicy food and chemical medicine.

Four Effects, One Plan

Gastrointestinal regeneration and restoration technique is one of the techniques of Human Body Regeneration and Restoration Science, which solves the problem of gastrointestinal aging in advance, the problem of ulcer healing with scar formation, and the problem of GI damage and constipation. Personal biotic clock diet plan is enacted according to personal condition of gastrointestinal biotic clock.

The First Effect: Solve the problem of gastrointestinal aging in advance

For human, even though we firstly see the skin getting old, while actually, it’s the gastrointestinal mucosa which firstly starting to age. More worse, GI aging leads to the aging of our whole life. The life of the gastrointestinal mucosa is maintained through continuous self renewal depending on gland tissues under mucosa. The renewal of mucosa is fulfilled through the proliferation and differentiation of the stem cells existing in the gland tissues. For example, there are about 250 cells in the intestine gland tissue and there are certain stem cells among these cells. When one generation of mucosa tissue cells getting old, these stem cells will produce and form a new generation of mucosa organ. Thus, the life of mucosa organ can be maintained actively and ensure the absorption of nutrients of human body. However, the self-renewal ability will decrease with the increase of age and the long-term damage of mucosa, the number of stem cells are getting fewer and fewer, which will slow down the renewal speed and the atrophy and further cause aging in advance of the mucosa organ. And the aging of mucosa organ will decrease the digestion and absorption ability, which will lead to primary diseases for other organs. The experiments proved that the nutrient composition in MEBO Gastrointestinal Capsule can promote the stem cells and potential regenerative cells inside the gastrointestinal mucosa to in situ regenerate gastrointestinal mucosa organ, which can then prevent the aging in advance of mucosa.

In daily life, the aging of gastrointestinal organs is recognized as easy to tired, listless, adiposity or marasmus and etc. Since the absorption and digestion ability have decreased, most mid-aged people find themselves troubled with the symptoms above. MEBO Gastrointestinal Capsule can largely retard aging and help people to maintain the absorption and digestion ability of mucosa and recover the functions of aged mucosa organs.

The Second Effect: Solve the problem of scar formation after gastrointestinal ulcer treatment

The healing of gastrointestinal ulcer and inflammation is not a puzzle any more with the development of Modern Medicine. However, the remaining problem is, the ulcers will always be healed with scar formation and the healed part doesn’t have physiological function and structure, which in one way will decrease the digestion and absorption function and in the other way will lead to abdominal discomfort or even stomachache and abdominal distension. MEBO Gastrointestinal Regeneration and Restoration technique can promote the residual stem cells and potential regenerative cells existing in ulcer wound to in situ regenerate new gastrointestinal mucosa organ, which will finally achieve ulcer healing without scar formation and recover all physiological structure and function completely.

The Third Effect: Solve the problem of damages caused by drinking, taking medicine and food

Drinking, taking medicine or even diet can cause gastrointestinal mucosa damages, which will lead to gastrointestinal function disorder or even maldigestion and malabsorption. The main reasons for diet to cause mucosa damages includes: people don’t know what best fits gastrointestinal mucosa and no preventive protection is carried out during digestion. When the mucosa is damaged, the nutrients can not be efficiently digested and absorbed and damages will be directly led to human body organs and cause the nutrient defect disease in advance. The self-protection of mucosa is mainly realized through the mucus released by GI organ and adhered to the surface of mucosa. When the mucus decreased or damaged, gastrointestinal mucosa will be damaged by gastric acid and pepsin. Even though the self-renew ability of GI mucosa is kind of strong, while the long-term damage irritation; the decrease of mucus renewal and long-term damage from food can also cause many GI diseases. MEBO Gastrointestinal Capsule used in the Gastrointestinal Regeneration and Restoration technique has the function of sticking to the gastrointestinal mucus, which can strengthen the protection function of mucus to mucosa. Experiment results proved that the administration of MEBO Gastrointestinal Capsule can prevent the stimulus of alcohol and chemical drugs from damaging mucosa.

The Fourth Effect: Solve the problem of constipation

Constipation, sounds very common, but will threat to life if serious. Especially for middle aged or aged persons, constipation will always initiate heart and cerebrovascular diseases. The medical divisions of constipation are as following: intractable constipation caused by birth defect, functional constipation caused by non-disease causes and organic constipation caused by diseases. There are few effective solutions to constipation so far worldwide. We divide constipation into two types: excluding the intractable constipation and birth defect, all the other types of constipation are caused by the abnormal cells in the defecation system. Basically after the application of normal biotic clock diet plan, constipation will not occur. What’s more, MEBO Gastrointestinal Capsule has the function of removing constipation. The gastrointestinal regeneration and restoration technique solves the constipation problem through the regeneration of muscular layer cells of intestine, rectum mucosa and intestinal wall.

One Plan: Biotic clock diet plan

The research shows that one of the main reasons causing gastrointestinal mucosa damage, ulcer, and chronic inflammation and aging in advance is that the biotic clock diet is not followed. So mastering the correct biotic clock diet is very important in order to retard aging and diet without damages to gastrointestinal mucosa. MEBO Gastrointestinal Regeneration and Restoration technique helps people to enact personal biotic clock diet plan according to individual physiological habits and conditions.

To further test the ability of the MEBO regenerative nutrients to facilitate cloning of physiologically correct tissue in vitro, experiments were conducted on explants of mouse intestine. Explants of mouse intestine were obtained and a few villi on the explants were excised and cultured in normal cell culture medium. The cultured explants were divided into two groups: test group and control group. The MEBO regenerative nutrients were added to the cultures in the test group, while the control group did not receive any supplement.

As shown in the panels in Figure7.4.4.1a-d, in the treatment group the explants not only survived but also grew new villi from the wound where the original villi had been removed. By day 6, nascent structure of villus started to appear on the wound, and by day 8, regeneration of multiple new villi with the same physiological structure as the original ones was complete. By contrast, explants in the control group gradually disintegrated and died in the culture (Figure7.4.4.2a-d).

So far our extensive literature search has not found reports of such results of regeneration of villus from explants cultured in vitro. These results are significant as they once again demonstrate that the MEBO regenerative nutrients are able to promote regeneration of injured tissue and organ, consistent with the clinical results obtained from wound and ulcer patients. Scientifically groundbreaking is the fact that under the condition provided by the MEBO regenerative nutrients, normal somatic cells can behave like stem cells to proliferate vigorously and differentiate orderly to produce multiple cell types which eventually form tissue with physiologically correct structure, both in vivo and in vitro.

These findings are illuminating in that the MEBO regenerative nutrients can be delivered not only topically but also systemically to facilitate regeneration of injured or aged tissue and organ in the body.

Built upon MEBO’s innovative technologies and products for the regeneration of wounded skin that leads to restoration of normal skin structure and function, MEBO has developed a series of products that are formulated for oral delivery and can be taken conveniently in the form of soft gel capsules by users to maintain the integrity of or to repair/regenerate the mucosal tissue without scar formation. Similar to the process of regeneration of the epithelium of the skin, repair or regeneration of damaged or eroded epithelium on the mucosal lining can be achieved by activating and sustaining intrinsic regenerative potential of the body and cultivating stem cells of the body in situ, i.e., right at the site of ulcerative lesion.

As shown below, the mucosal tissues of those who suffered from various GI disorders (such as gastric ulcer, duodenal ulcer and erosive gastritis due to the side effects of chemotherapy and radiation) can be regenerated after a month of taking the MEBO regenerative nutrients. The newly regenerated mucosa is scarless.

After the composition of MEBO GIC entering the gastrointestinal tract, the composition will mix with mucus and form more solid prevention layer to prevent the stimulus of food, gastric acid and other destroying factors. If damage occurs, the composition will search it immediately and adheres to the damaged surface because of the high affinity. Then a protection layer will form to prevent secondary damage. So this product can not only protect gastrointestinal mucosa from damage, but also assure the normal digestion and absorption function.

GIC can prevent gastrointestinal mucosa from the damage of the habit disturbance such as: drinking, smoking, spicy and cold food.

The experimental animal: 10-month-old male albino rat with the average life of 480 days. The experiment lasted for 526 days. The experiment proves: MEBO Gastrointestinal Regeneration & Restoration technique (including MEBO GIC) can maintain the vigorous life of the gastrointestinal mucosa, the related gland tissues, muscular layer of the smooth muscle. However, animals without the administration of GIC appear: the atrophy of gastrointestinal mucosa, obvious aging, obviously decreased gland, thinning of muscular layer of smooth muscle.

The experimental results are as follows:

7.3 Regenerative restoration of GI tract in humans

As shown below in Figures 7.3.1 and 7.3.2, healthy adult humans, even if having degenerated/atrophic shortened villi on the GI lining due to aging, can still benefit from the regenerative power of the MEBO nutrients which promote re-growth of lush, longer villi (which are associated with a more youthful human body). By using the MEBO nutrients to regenerate the GI lining without scar formation, the GI system is revitalized functionally to allow better absorption of nutrients to the body, which, in turn, leads to nourishment of the body systematically.