Use of short repetition time (TR) relative to metabolite
T1s is common in 31P MRS of biological samples. However, spectral resonances
acquired with short TR values exhibit saturation effects that have
traditionally been corrected using saturation factors. This approach does not
account for the chemical exchange exhibited in biological systems which may
lead to large errors. Here, we describe an approach to metabolite
quantification based on performing ongoing dual&#61485;angle measurements
(O-DAM) in the setting of chemical-kinetic changes. Results showed that O-DAM
correction can permit accurate monitoring of metabolite concentrations even in
the setting of chemical exchange and changing chemical-kinetic parameters.