Purchase

Description

Background Information

BIOTRANS is an amphoteric nylon membrane with a surface chemistry comprising 50% amino and 50% carboxyl groups. This provides a hydrophilic membrane with an isoelectric point at pH 6.5. BIOTRANS membranes have a high binding capacity for biomolecules in transfer applications and offer greater sensitivity, improved nucleic acid retention and superior handling characteristics over traditional nitrocellulose.

Applications

Biotrans Nylon Membranes can be use for Plaque and Colony Lifts & Southern and Northern blotting.

BIOTRANS™ membranes are reinforced nylon 66 membranes for use in DNA, RNA and protein transfers. The physical characteristics of BIOTRANS membranes make them especially useful as a transfer medium. BIOTRANS membranes provide excellent band resolution due to their uniform and carefully controlled pore rating. The membranes are inherently hydrophilic and therefore do not require prewetting prior to use. BIOTRANS membranes are also heat resistant and solvent-resistant and will not shrink, crack or tear thus allowing multiple hybridization cycles to be performed.

Properties

Suitability

Suitable for Southern and Northern blotting, colony and plaque lifts and Dot-/Slot-Blots

Store at 2-8 °C. Membrane Handling: BIOTRANS membranes are naturally hydrophilic and do not require pre-wetting before use. The membranes are mechanically very strong and resistant to tearing or cracking making removal from the agarose gel particularly easy. BIOTRANS membranes should be handled using gloves or forceps to prevent membrane contamination. Either scissors or a sharp scalpel must be used to cut the membrane.

Suitability

Suitable for Southern and Northern blotting, colony and plaque lifts and Dot-/Slot-Blots