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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 20% Fetal Bovine Serum (FBS). Spilt confluent cultures 1:3 to 1:10 i.e. seeding at 1-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Established in 1974 from a mouse brain neoplasm tumour induced by nitrosoethylurea and possess many properties characteristic of smooth muscle. Since then it has been suggested that BC3H1 is a skeletal line of ectodermal origin that is defective for commitment to terminal differentiation. The two distinct morphologies typical of this cell line are large spheroid cells and smooth-muscle-like cells. The fusiform is the more common.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)