BioTechnology: An Indian JournalISSN (PRINT): 0974-7435

The Production of Xylanase Enzyme (E.C. Number=3.2.1.8) Using Solid Substrate Fermentation

The production of Xylanase enzyme (E.C. number=3.2.1.8) was studied using solid substrate fermentation. Various solid substrates were selected as a solid substrate for Xylanase production using SSF. Optimum xylanase activity was observed when Pea peel has been used as solid substrate. The production of the xylanase is carried out some other solid agricultural waste because. Agro industrial waste are a good source of nutrition for the growth of the microorganisms as they are rich in carbon source and agro-industrial wastes such as wheat bran, sugarcane bagasse, corn cob, rice bran and wheat straw are abundantly available and cheapest natural carbon sources. The present study is an attempt for process optimization for xylanase production using agro industrial waste as a sole carbon source. The production of the enzyme xylanase has industrial use and it can be used for many commercial purposes including chlorine free bleaching of the wood pulp prior to papermaking. Xylanases can also be used as food additives in poultry, in wheat flour for improving the handling of dough and increasing the quality of baked products, it is also used for the extraction of coffee, extraction of plant oils and starch. Different physical and chemical parameters which affects the production of xylanase has been optimized by batch experiment as well as using statistical tool i.e., Design-Expert. Experimental outcomes showed that agro-industrial residue having excellent potential for the production of industrial important enzyme i.e., Xylnase.