One- or Two-Step RT-PCR

We offer a complete portfolio for qualitative PCR and one-step or two-step RT-PCR. Our OneStep (Ahead) RT-PCR Kits offer the highest sensitivity and specificity from cDNA synthesis to the subsequent PCR reaction in just one step. The new QIAGEN OneStep Ahead RT-PCR Kit also includes a novel two-phase hot-start procedure for convenient room-temperature setup, making it suitable for automated/robotic workflows. Additionally, it offers the fastest cycling protocol, elevated fidelity, a visual pipetting control and many more compelling new features to help you achieve instant success. If you prefer to separate the cDNA synthesis step from the PCR, use our Omniscript and Sensiscript Kits for cDNA synthesis and our HotStarTaq Kits/mastermixes or Multiplex PCR Kits for PCR.