دانشگاه تهرانIranian Journal of Veterinary Medicine2251-88942252-05547320131001Genetic and phylogenetic analysis of the ribonucleoprotein complex genes of H9N2 avian influenza viruses isolated from commercial poultry in Iran159168ENMohsenBashashatiDepartment of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iranmbashashati@ut.ac.irMahdiVasfimarandiDepartment of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iranmvmarand@ut.ac.irMohammad HassanBozorgmehri FardDepartment of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iranmh.b.fard@ut.ac.irFarhidHemmatzadehSchool of Animal and Veterinary Sciences, University of Adelaide, Adelaide, South Australia, Australiafarhid.hemmatzadeh@adelaide.edu.auFereshtehSabouriDepartment of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iranfereshtehsabouri@gmail.com10.22059/ijvm.2013.35965BACKGROUND: The H9N2 subtype of avian influenzaviruses (AIVs) has been isolated in multiple avian species inmany European, Asian, African and American countries. Sincethe first outbreak of H9N2 virus in Iran in 1998, this virus haswidely circulated throughout the country, resulting in majoreconomic losses in chicken flocks. Several amino acids in thevirus ribonucleoprotein (RNP) complex including the nucleoprotein(NP) and polymerase (PB2, PB1 and PA) proteins areassociated with host range and virulence. OBJECTIVES: Our aimwas to understand the molecular characterization of RNPcomplex proteins of Iranian H9N2 subtype isolates. METHODS:The full length nucleotide sequences of RNP complex genes oftwo strains designated as Ck/IR/ZMT-101/98 and Ck/IR/EBGV-88/10 were amplified and sequenced. RESULTS: The phylogeneticanalysis revealed that both strains were located indifferent sub-lineages. However, based on the genetic similarities,PB1, PAand NP genes of Ck/IR/EBGV-88/10 strain had aclose relationship with a H7N3 subtype strain, isolated fromPakistan. Most positions of RNP proteins contained amino acidstypical of avian determinants of host range. The results showedthat the Iranian RNP complex genes have undergone geneticreassortment. CONCLUSIONS: Continuous AIV monitoring inpoultry industry would help to obtain more information aboutgenetic variation of H9N2 viruses and possible emergence ofvirulent and/or pandemic viruses.avian influenza virus,H9N2,poultry,ribonucleoproteinhttps://ijvm.ut.ac.ir/article_35965.htmlhttps://ijvm.ut.ac.ir/article_35965_9e88f7752dfec61b1f6720c2098dee7b.pdfدانشگاه تهرانIranian Journal of Veterinary Medicine2251-88942252-05547320131001Bacterial contamination of dead-in-shell embryos in ostrich hatcheries and antimicrobial resistance patterns of isolated Escherichia coli169175ENAryaRezaei FarDepartment of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Irana_rezaeifar@yahoo.comSeyed MostafaPeighambariDepartment of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iranmpeigham@ut.ac.irAvestaSadrzadehDepartment of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Garmsar Branch,
Garmsar, Iranavestasadr@gmail.comMahdiAskari BadoueiDepartment of Microbiology, Faculty of Veterinary Medicine, Islamic Azad University, Garmsar Branch,
Garmsar, Iranmic.consult@gmail.com10.22059/ijvm.2013.35967BACKGROUND:The bacterial contamination of fertile eggs isthe most common cause of embryonic death in ostrich hatcheryunits leading to financial loss in ostrich industry. OBJECTIVES:The aim of this research was to investigate the bacterialcontamination status, with emphasis on Escherichia coli, ofostrich hatcheries and the antimicrobial resistance profile ofisolated Escherichia coli. METHODS:Atotal of 120 ostrich eggswith dead embryos, at weekly intervals, were collected fromthree ostrich hatcheries. The dead embryos were sent tolaboratory and samples were collected aseptically from differentorgans. Bacterial detection and identification were performed byusing standard bacteriological and biochemical techniques.Antimicrobial susceptibility test was carried out by agar diskdiffusionmethod against 27 antimicrobial agents. RESULTS:Different types of bacteria were isolated from 56 eggs (46.7%).Twenty-four ostrich eggs were shown to carry E. coli. In someeggs, in addition to yolk sac, E. coli was also isolated frommeconium, liver, or heart blood which increased the total numberof E. coli isolates to 32. All E. coli isolates were susceptible totrimethoprim + sulphamethoxazole, danofloxacin, and flumequine,whereas all were resistant to carbenicillin and erythromycin.Resistance to other agents was variable. Multi-drugresistance pattern was found among all E. coli isolates andincluded 2 to 12 drugs. Thirty-two E. coli isolates generated 30different resistance profiles against 27 antimicrobial drugs.CONCLUSIONS: This was the first comprehensive reportregarding the bacterial, particularly Escherichia coli, contaminationof dead-in-shell ostrich embryos and antimicrobial resistancestatus of the Escherichia coli isolates from ostrich eggs inIran.antimicrobial resistance,embryonic death,Escherichia coli,Hatchery,Ostrichhttps://ijvm.ut.ac.ir/article_35967.htmlhttps://ijvm.ut.ac.ir/article_35967_9e469988fb3252aa28ec315738d3b4e9.pdfدانشگاه تهرانIranian Journal of Veterinary Medicine2251-88942252-05547320131001Serological and bacteriological study of leptospirosis in dairy herds and feedlot in Tehran suburbs177183ENShahramMalekiDepartment of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iranshmaleki9@gmail.comGholamrezaAbdollahpourDepartment of Hygiene and Food control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Irangreza@ut.ac.irAlirezaBahonarDepartment of Hygiene and Food control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iranabahonar@ut.ac.ir10.22059/ijvm.2013.35968BACKGROUND: Leptospirosis is a worldwide zoonosis causedby Leptospira interrogans. Leptospirosis results in decreasedmilk production, abortion, stillbirth, infertility and mortality,which causes financial loss in the cattle industry. OBJECTIVES:The aim of this research was to perform a serological andbacteriological study of leptospirosis in 6 industrial dairy herdsand 3 feedlots with previous records of leptospirosis in Tehransuburbs in 2011-2012. METHODS: For the purpose of this study,408 blood samples from dairy cattle and 154 blood samples fromfeedlots were collected using sterile 10ml venoject vacutainersfrom tail vein. Two months later, 118 urine samples werecollected from 20% of the two groups of serological negative andpositive animals. All serum samples were serologically tested bymicroscopic agglutination test (MAT), a standard method forserological diagnosis of leptospirosis. The serum samples weretested for antibodies against five live antigens of Leptospirainterrogans serovars: Pomona, Grippotyphosa, Hardjo, Icterohaemorrhagiaeand Canicola. Urine samples were used forbacteriological isolation of Leptospira spp. RESULTS: Serologicalresults showed that 228 (40.6%) of animals had a positivereaction against one or more serovars. The most prevalentLeptospira serovars was Pomona 118 (40.3%) and the leastprevalent was Canicola 4 (1.4%). The most prevalent titer was1:100, and the highest titer was 1:1600. Also the mostseropositive cases were observed in 3 to 4-year-old cows.Bacteriological results revealed that in 11 (9.3%) urine samplesLeptospira spp. were isolated, all taken from one feedlot farm.According to the history taken from each farm, the main riskfactors were the presence of rodents and low hygienic conditionsof the farms. CONCLUSIONS: The results of this study revealedthat cows could have a major role in maintaining Pomona,Grippotyphosa and Hardjo serovars; indeed, they are a potentialzoonotic risk to slaughter house workers, meat inspectors,milkers and farmers.bacteriology,cattle,leptospirosis,microscopic agglutination test,serologyhttps://ijvm.ut.ac.ir/article_35968.htmlhttps://ijvm.ut.ac.ir/article_35968_ce805ddb61a2a853949ebbc5f4e7f6cc.pdfدانشگاه تهرانIranian Journal of Veterinary Medicine2251-88942252-05547320131001Aserological survey on antibodies against West Nile virus in horses of Khuzestan province185191ENMahdiPourmahdiDepartment of Food Hygiene, Faculty of Veterinary Medicine, University of Shahid Chamran, Ahvaz, Iranpourmahdim@gmail.comAlirezaGhadrdan MashadiDepartment of Clinical Sciences, Faculty of Veterinary Medicine, University of Shahid Chamran, Ahvaz, IranMasoudrezaSeifi Abad ShapouriDepartment of Pathobiology, Faculty of Veterinary Medicine, University of Shahid Chamran, Ahvaz, IranMarziyehZeinvandGraduated from the Faculty of Veterinary Medicine, University of Shahid Chamran, Ahvaz, Iran10.22059/ijvm.2013.35969BACKGROUND: West Nile virus (WNV) is a vector-borneagent that is maintained within a bird-mosquito cycle. In humansand equids, infection by this agent is usually asymptomatic, orcharacterized by a mild febrile illness. However, fatal meningoencephalitisor encephalitis may occur. OBJECTIVES:The aim ofthis study was to evaluate the prevalence of WNVinfection andcorrelation of this organism with host and environmentaldeterminants in horses in Khuzestan province. METHODS: In2011-2012, serum samples of 155 horses were randomly collectedfrom 7 zones of Khuzestan province and were examined byELISAassay. RESULTS: Seroprevalence of WNVinfection was70.3% (95% CI: 63.1-77.5%). Statistical analysis showed thatage, zone, presence of lake, type of bed, time of sampling, stayingout of the stable after sunset and the method of insect control aresignificantly associated with infection (p0.05).CONCLUSIONS: The results of the present study confirm that theWNV infection exists in Khuzestan province. Considering thelocal weather conditions and the facility of vector-bornetransmission, the health authorities should take measures toprevent and control the infection.Epidemiology,horse,West Nile virushttps://ijvm.ut.ac.ir/article_35969.htmlhttps://ijvm.ut.ac.ir/article_35969_9babb08d9fd72affd2b192bc8856033f.pdfدانشگاه تهرانIranian Journal of Veterinary Medicine2251-88942252-05547320131001Isolation, characterization and transduction of canine bone marrow-derived mesenchymal stem cells (cBM-MSCs)193199ENMahdiehRezaeiDepartment of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iranmahdiehrrezaei@gmail.comShahramJamshidiDepartment of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iranshjamshidi@ut.ac.irAnnaSaffarpourDepartment of Periodontology, International Campus School of Dentistry, Tehran University of Medical
Sciences, Tehran, Irananna_1359_sa@yahoo.comMahdiAshouriDepartment of Oral and Maxillofacial Pathology, Faculty of Dentistry, Shahed University of Medical Sciences,
Tehran, Iranm_ashouri86@yahoo.comDavoodSharifiDepartment of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Irandsharifi@ut.ac.irHamedZamankhan MalayeriDepartment of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iranhamed_zamankhan@yahoo.comNaqaTamimiDepartment of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Irannaqavet2005@yahoo.comAmir RezaRoknDental Implant Research Center and Department of Periodontics, School of Dentistry, Tehran University of
Medical Sciences, Tehran, Iranm.rezaei245@yahoo.com10.22059/ijvm.2013.35970BACKGROUND: Stem cell therapy in small animal medicineis still in its infancy and few in vitro and in vivo research projectsregarding animal Mesenchymal Stem Cells (MSCs) have beencarried out. On the other hand, Cell tracking is the first step of thecell-based therapies and is essential to recognize cell fate posttransplantation. OBJECTIVES: The aim of this study was toisolate, characterize, and transduce cBM-MSCs. METHODS:Canine Bone Marrow-derived Mesenchymal Stem Cells (cBMMSCs)were isolated from bone marrow of dogs andcharacterized based on morphology, differentiation capacities,and surface marker expressions. For the first time, we labeledcBM-MSCs by GFP-encoding lentiviral vector to track them.RESULTS: cBM-MSCs were successfully isolated and proliferated.Morphologically, these cells were similar to otherMSCs from other sources and species and were able todifferentiate into osteocytes and adipocytes. cBM-MSCsexpressed surface marker CD44 but were not able to expressCD34. Approximately, 70% of cells efficaciously expressedGFPafter labeling; CONCLUSIONS:We found that GFP labelingis an easy and effective technique to track transplanted cBMMSCs.Our results also provide fundamental information aboutcanine BM-MSCs in order to use in veterinary medicine.canine Bone Marrow-derived
Mesenchymal Stem Cells (cBMMSCs),characterization,green
fluorescent protein (GFP),transductionhttps://ijvm.ut.ac.ir/article_35970.htmlhttps://ijvm.ut.ac.ir/article_35970_8db68c9b35f46b1d2c359a90aa8b02c9.pdfدانشگاه تهرانIranian Journal of Veterinary Medicine2251-88942252-05547320131001Correlations among homocysteine, cardiac troponin I and cardiac enzymes in different ages of clinically healthy male dromedary camels201206ENMehrdadPourjafarDepartment of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Irandmp4m@yahoo.comAliasgharChalmehDepartment of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iranachalmeh81@gmail.comKhalilBadieiDepartment of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iranbadiei33@gmail.comSaeidNazifiDepartment of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Irannazifi@shirazu.ac.irSamanehKeshavarzDepartment of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iransamanek22@yahoo.comMojtabaNaghibDepartment of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Irandr.mojtabanaghib@gmail.com10.22059/ijvm.2013.35971BACKGROUND: Information regarding serum biochemicalprofile can reflect cardiovascular performance in animals.Although studies have evaluated the inter-relationship amongcardiovascular biomarkers in animals and human beings, thereare no reports of such a probable relationship in camelids.OBJECTIVES: The aim of the present study was to provide dataon the correlations among cardiovascular biomarkers indifferent ages of clinically healthy male dromedary camels toprovide a basis for assessing cardiac muscle healthiness in thisspecies. METHODS: Thirty clinically healthy dromedary camels(Camelus dromedarius) were selected and divided into four agegroups including 1-3 (n=7), 4-6 (n=7), 7-9 (n=8), and above 10(n=8) years old. Blood samples were collected and sera wereseparated. Serum concentrations of homocysteine (Hcy),cardiac troponin I (cTnI), creatine kinase-myocardial specificisoenzymes (CK-MB), lactate dehydrogenase (LDH), alanineaminotransferase (ALT) and aspartate aminotransferase (AST)were evaluated. RESULTS: The results of the present studyshowed that there were significant correlations among cTnI andCK-MB (r=-0.853; p=0.015) and Hcy (r=0.916; p=0.004) in the4 to 6-years-old group of clinically healthy male dromedarycamels. LDH was significantly correlated with CK-MB in the 7to 9-year-old group (r=-0.710; p=0.045). There were nosignificant correlations among different factors of 1-3 and above10-year-old groups (p>0.05). CONCLUSIONS:The data providedhere is the first report on cardiac health assessment parameters indromedary camels. Moreover, the data is valuable in camelracing clubs, when an overall cardiac health and fitness is to beassessed. The correlation reported here might also be helpful foreasier analysis of cardiac health status in dromedary camels. Thedata may be useful for assessing suspected cases of myocardialdiseases and its changes maybe of prognostic value.cardiac biomarkers,correlation,dromedary camelshttps://ijvm.ut.ac.ir/article_35971.htmlhttps://ijvm.ut.ac.ir/article_35971_c1f3d116a8807e124062cb36d3e5b59f.pdfدانشگاه تهرانIranian Journal of Veterinary Medicine2251-88942252-05547320131001Detection of Coxeilla brunetii in bulk tank milk samples from dairy bovine farms using nested-PCR in Qom, Iran, 2011207211ENArashGhalyanchi LangeroudiDepartment of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran,
Iranghalyana@ut.ac.irNargesBabkhaniDepartment of Microbiology, Qom Branch, Islamic Azad University, Qom, Iranbabakhani.narges@gmail.comMohammad RezaZolfaghariDepartment of Microbiology, Qom Branch, Islamic Azad University, Qom, Iranarashghalyanchi@gmail.comKeivanMajidzadeh ArbadiliTASNIM Biotechnology Research Center, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Irankmajidzadeh@razi.tums.ac.irAbbasMorovvatiDepartment of Microbiology, Qom Branch, Islamic Azad University, Qom, Iranabbasmorovvati@gmail.comMohammadSoleimaniDepartment of Microbiology, Qom Branch ,Islamic Azad University, Qom, Iransoleimanidor@yahoo.com10.22059/ijvm.2013.35972BACKGROUND: Q fever is a zoonotic disease caused byCoxiella burnetii, a species of bacteria that is distributedworldwide. In cattle, Coxiella burnetii infections are generallyasymptomatic but can also be associated with reproductivedisorders. OBJECTIVES: The aim of this study was to achievemolecular detection of Coxiella burnetii in dairy bovine milkfarms using Nested PCR in Qom province, Iran. METHODS:From January to February 2011 (winter) and July to September2011(summer) a total of 100 bovine bulk milk samples wereequally collected from five areas of Qom. The nested PCR assayused to screen for C. burnetii was designed from the nucleotidesequence of the com1 gene encodin a 27-kD outer membraneprotein (OMP). RESULTS: In this study, 14% (14 of 100) of bulkmilk were positive. CONCLUSIONS: These results support thehypothesis of high prevalence and endemic pattern of Q fever inQom province of Iran.Coxeilla brunetii,Q fever,nested
PCRhttps://ijvm.ut.ac.ir/article_35972.htmlhttps://ijvm.ut.ac.ir/article_35972_2904fe92563ec35289d7bf19a60d6e3e.pdfدانشگاه تهرانIranian Journal of Veterinary Medicine2251-88942252-05547320131001Blood and tissue levels of diazinon in rabbit following a subacute dermal exposure to incremental doses213219ENHoseinaliArabDepartment of Pharmacology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iranharab@ut.ac.irMasoudGoudarziDepartment of Pharmacology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iranmasoudgoudarzi@yahoo.comMohammadkazemKoohiDepartemt of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iranmkkoohi@yahoo.comGholamrezaShamsDepartment of Pharmacology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Irangholamrezashams@yahoo.com10.22059/ijvm.2013.35973BACKGROUND:Uncontrolled application of diazinon (DZN)can cause environmental contamination and adverse healtheffects on humans or animals. OBJECTIVES:This study aimed toinvestigate the toxic effects and the level of DZN in serum andtissues of rabbits following a sub acute dermal exposure totoxicant. METHODS:Different doses of DZN were applied dailyto New Zealand rabbits through the ear skin in incremental dosesfor 4 weeks. Blood samples were collected at the beginning andthe end of each dose-week period. Tissue samples were collectedfrom brain, muscle, kidney and liver on day 28, after euthanizingthe rabbits. DZN contents of the blood and tissue samples weremeasured using a reversed phase HPLC system. RESULTS:Clinical observations indicated signs of toxicity in the animalsexposed to DZN as shown by diarrhea and body weight loss fromday twenty. The level of DZN in the blood elevated withenhancing exposure time and reached the highest level at the endof the fourth week (0.620±0.26ppm). The highest level of DZNwas found in the brain tissue (0341±0.015 ppm). CONCLUSIONS:The results of this study revealed the tissue accumulation andsubsequent toxic effects of DZN following the subacute dermalexposure to the toxicant. It suggests that the determination of thetoxicant level in the serum or tissue can be a monitoring methodfor the detection of the contamination rate.blood and tissue level,dermal
exposure,Diazinon,rabbithttps://ijvm.ut.ac.ir/article_35973.htmlhttps://ijvm.ut.ac.ir/article_35973_a776751fbba28300348c1a88efd2ab20.pdfدانشگاه تهرانIranian Journal of Veterinary Medicine2251-88942252-05547320131001Antibiotic residues and aflatoxin M1 contamination in milk powder used in Tehran dairy factories, Iran221226ENNeginNooriDepartment of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Irannnoori@ut.ac.irGuityKarimDepartment of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Irangkarim@ut.ac.irMahyarRaeesianDepartment of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran- Iranmahyarraeesian_63@yahoo.comHamidKhaneghahi AbyanehDepartment of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran- Iranhamidkhaneghahi@yahoo.comAlirezaBahonarDepartment of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran- Iranabahonar@ut.ac.irAfshinAkhondzadeh BastiDepartment of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran- Iranaakhond@ut.ac.irFreshtehGhadamiDepartment of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran- Iranghadamif@ut.ac.ir10.22059/ijvm.2013.35974BACKGROUND: The presence of aflatoxin M1 (AFM1) andantibiotic residues in milk and milk products is a public healthconcern. Milk and milk powder have the potential forintroducing AFM1 and antibiotic into human diet. In recentyears, milk powder has been used on a large scale in dairyfactories. Consequently, antibiotic residues and aflatoxincontamination control in these products has gained importance.OBJECTIVES: The aim of this survey was to determine the levelof β-lactam and tetracycline antibiotic residues and also AFM1contamination of milk powder used in Tehran dairy factories.METHODS: During 12 months (September 2011 to September2012), 240 samples of milk powder were collected from tenTehran dairy factories. All samples were analyzed for thepresence of AFM1 using ELISA technique. In addition,antibiotic residues were determined by BetaStar Combo test, arapid assay for both β-lactam and tetracycline antibiotics.RESULTS: The samples depicted positive results i.e. 30% and17.5% for β-lactam and tetracycline antibiotics, respectively.Also, AFM1 was found in 155 cases (64.6%) with an averageconcentration of 29.85 ± 18.99 ng/ L. CONCLUSIONS:The resultsshowed the milk powder used by dairy factories is safe in respectof AFM1 contamination and antibiotic residues in Tehran.aflatoxin M1,antibiotic residues,milk powderhttps://ijvm.ut.ac.ir/article_35974.htmlhttps://ijvm.ut.ac.ir/article_35974_d4c6b47e03b5c14f86fb8ff34a57a2fb.pdfدانشگاه تهرانIranian Journal of Veterinary Medicine2251-88942252-05547320131001First report on Anaplasma platys infection in a dog in the Philippines227231ENAdrianYbanez1Department of Veterinary Clinical Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro
city Hokkaido, Japan
2United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan
3College of Veterinary Medicine and Department of Animal Science, Visayas State University, Visca, Baybay City,
Philippinesdr.adrianpybanez@gmail.com10.22059/ijvm.2013.35975Anaplasma platys, previously known as Ehrlichia platys,which causes infectious canine cyclic thrombocytopenia, has notbeen extensively studied in Southeast Asia. Recent reports in theregion have been limited to ticks and dogs in Thailand, and toticks in the Philippines. In this report, DNAfragments of A. platyswere molecularly detected in a dog in the Philippines. Apitbullpuppy exhibited pancytopenia (WBC: 3.5 x103/μL, PCV:11.5%, RBC: 1.2 x106/μL, HGB: 3.7 g/dL, platelet count: 24x103/μL), hepatitis, elevated alanine amino transferase (127u/L), and splenomegaly. Inclusion bodies in the platelets werealso seen in the blood smears of this case. A. platys sequencesobtained in the present study revealed 100% homology with A.platys previously detected in a Rhipicephalus sanguineus tick inthe country. This study documents the first reported case of A.platys infection in dogs in the Philippines, and adds knowledgeto the current vector-borne disease epidemiology in SoutheastAsia.Anaplasma platys,dog,Epidemiologyhttps://ijvm.ut.ac.ir/article_35975.htmlhttps://ijvm.ut.ac.ir/article_35975_f3a612d0dba7f93e9a844117df97ad12.pdf