Scientists show a link between intestinal bacteria and depression

Exploring the role of intestinal microbiota in the altered behavior that is a consequence of early life stress

Scientists from the Farncombe Family Digestive Health Research Institute at McMaster University have discovered that intestinal bacteria play an important role in inducing anxiety and depression.

The new study, published in Nature Communications, is the first to explore the role of intestinal microbiota in the altered behavior that is a consequence of early life stress.

"We have shown for the first time in an established mouse model of anxiety and depression that bacteria play a crucial role in inducing this abnormal behavior," said Premysl Bercik, senior author of the paper and an associate professor of medicine with McMaster's Michael G. DeGroote School of Medicine. "But it's not only bacteria, it's the altered bi-directional communication between the stressed host -- mice subjected to early life stress -- and its microbiota, that leads to anxiety and depression."

It has been known for some time that intestinal bacteria can affect behavior, but much of the previous research has used healthy, normal mice, said Bercik.

In this study, researchers subjected mice to early life stress with a procedure of maternal separation, meaning that from day three to 21, newborn mice were separated for three hours each day from their mothers and then put back with them.

First, Bercik and his team confirmed that conventional mice with complex microbiota, which had been maternally separated, displayed anxiety and depression-like behavior, with abnormal levels of the stress hormone corticosterone. These mice also showed gut dysfunction based on the release of a major neurotransmitter, acetylcholine.

Then, they repeated the same experiment in germ-free conditions and found that in the absence of bacteria mice which were maternally separated still have altered stress hormone levels and gut dysfunction, but they behaved similar to the control mice, not showing any signs of anxiety or depression.

Next, they found that when the maternally separated germ-free mice are colonized with bacteria from control mice, the bacterial composition and metabolic activity changed within several weeks, and the mice started exhibiting anxiety and depression.

"However, if we transfer the bacteria from stressed mice into non stressed germ-free mice, no abnormalities are observed. This suggests that in this model, both host and microbial factors are required for the development of anxiety and depression-like behavior. Neonatal stress leads to increased stress reactivity and gut dysfunction that changes the gut microbiota which, in turn, alters brain function," said Bercik.

He said that with this new research, "We are starting to explain the complex mechanisms of interaction and dynamics between the gut microbiota and its host. Our data show that relatively minor changes in microbiota profiles or its metabolic activity induced by neonatal stress can have profound effects on host behavior in adulthood."

Bercik said this is another step in understanding how microbiota can shape host behaviour, and that it may extend the original observations into the field of psychiatric disorders.

"It would be important to determine whether this also applies to humans. For instance, whether we can detect abnormal microbiota profiles or different microbial metabolic activity in patients with primary psychiatric disorders, like anxiety and depression," said Bercik.

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“One of the most concerning aspects of our findings is that we found a significant portion of responders have new onset cognitive impairment when many of them were cognitively normal within just the past few years.”