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Sigma offers over 200 modifications for both DNA and RNA oligonucleotides and probes. Sigma can attach fluorescent dyes to oligos at the 5'-end, 3'-end and internal positions. Our product offering includes common dye modifiers such as 6-FAM™, HEX™, and TET™, as well as exotic modifications.

Phosphorothioates

Phosphorothioates (or S-oligos) are a variant of normal DNA in which one of the non-bridging oxygens is replaced by sulfur. The sulfurization of the internucleotide bond creates a molecule that is resistant to cellular nuclease degradation. Sulfur bonds can be introduced at the 5’, 3’, or internal positions.

Minimum Yield Guarantees

Modified Oligos (O.D./µg)*

Scale (µmole)

Desalt

Cartridge

HPLC

PAGE

0.05

2/60

0.4/12

0.4/12

0.2/6

0.2

5/150

1/30

1/30

0.4/12

1.0

16/480

5/150

5/150

2/60

10

160/4,800

NA

52/1,560

NA

15

240/7,200

NA

76/2,280

NA

*Guarantees are for 20 mers or longer. Oligos shorter than 20 bases will have proportionally fewer ODs. Microgram amounts are calculated based on a 30 μg/OD value. If the μg/OD value is less than 30, the reported μg value will be proportionally less. Phosphorothioated yields are 70% of the above stated values. Post-synthesis modifications may yield 50% less than the above stated values.

Non-Modified Phosphorothioates (S-Oligos) (O.D./µg )*

Scale (µmole)

Desalt

Cartridge

HPLC

PAGE

0.05

3.5/105

0.7/21

0.7/21

0.35/10.5

0.2

8.4/252

2.1/63

1.75/52.5

0.7/21

1.0

28/840

8.4/252

9.1/273

3.5/105

10

280/8,400

NA

91/2,730

NA

15

420/12,600

NA

133/3,990

NA

*Guarantees are for 20 mers or longer. Oligos shorter than 20 bases will have proportionally fewer ODs. Microgram amounts are calculated based on a 30 μg/OD value. If the μg/OD value is less than 30, the reported μg value will be proportionally less.