Why the cotyledons are removed from seedlings for performing cultural experiments ?

Cotyledons are reserves of microelements, therefore they can interefere with the culture and make the study difficult. Hence, they are removed from the seedlings in the culture experiments.

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What is Hidden hunger in the case of plants?

Hidden hunger refers to a situation in which a crop needs more of a given nutrient yet has shown no deficiency symptoms. The nutrient content is above the deficiency symptom zone but still considerably needed for optimum crop production

The nitrogenase enzyme is very sensitive to the molecular oxygen. It requires anaerobic conditions to work. The leg-haemoglobin in the nodule acts as an oxygen scavenger just meainting the anaerobic condition needed by the enzyme.