Children who were negative in all three laboratory tests were not investigated further, as they Nike Blazer White UK had a very low probability of having coeliac disease, and biopsy was considered unethical in such children.The samples were number coded and sent to Heim Pl Children's Hospital, Budapest, Hungary. To check for correct sampling and to perform an Nike Blazer Mid Suede Mens interobserver comparison between the nurses and the laboratory, the nurses also submitted any diluted blood left over from the rapid test.
Parents gave written informed consent to the blood sampling and screening. We sought separate consent for the confirmatory Nike Blazer Print UK Mens biopsy in children with positive test results.Rapid coeliac antibody testing Nurses used the Biocard coeliac disease test kit (AniBiotech) and evaluated results after five minutes but not later than 10 minutes, as instructed by the manufacturer. This immunochromatographic test detects IgA antibodies to tissue transglutaminase in whole blood with the aid of transglutaminase antigen contained within the erythrocytes in the sample being tested.141516 In brief, the blood sample is taken into a 10 l capillary, which is then inserted into a tube with haemolysing buffer to liberate the transglutaminase from the erythrocytes, and three drops are added to the test cassette.

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