Contents

Plan for Day 2

Group 1 should arrive by 1:05 pm at the latest and immediately go to the tissue culture room. When everyone has arrived, we will thaw your cells in the water bath. After you have finished your culture preparations (ideally by 3 pm), you can take a 10 minute break to refresh your minds, and then will take a short quiz.

Group 2 should arrive by 2:45 pm at the latest and will begin by taking the quiz. If all goes well, you will begin working in the tissue culture room at 3 pm. If your culture preparations that involve a special physical set-up, you can come earlier and work in the extra tissue culture hood.

Note that you may be asked to switch your group number based on grouping together people working with the same type of cells, asking people who need special reagents/equipment to go in the second group, etc.

T/R

Arrival time (at latest!)

1:05 pm

2:45 pm

Team colour 1

Blue

Yellow

Team colour 2

Red

Green

Team colour 3

Orange

Purple

Team colour 4

Platinum

W/F

Arrival time (at latest!)

1:05 pm

2:45 pm

Team colour 1

Pink

Red

Team colour 2

Green

Blue

Team colour 3

Orange

Team colour 4

Yellow

Designs (T/R)

Purple: Our plan is to culture chondrocytes under standard system conditions, but add an insulin supplement to the media at two concentrations higher than that already present in the ITS/FCS media. Insulin has been found to inhibit dedifferentiation of chondrocytes to the fibroblast state. We think our first 3D sample, subjected to 10 ug/day of insulin will inhibit dedifferentiation less than our second 3D sample, subjected to 20 ug/day, because a higher concentration of insulin will result in a larger inhibitory effect in the cell culture.

Designs (W/F)

Orange: We propose to study the effect of varying initial cell densities to determine an optimal starting density for the maintenance of chondrocyte differentiation. We hypothesize that lower cell density will result in a slower rate of central viability loss from due to the fact that in high density, cells can signal those around them and potentially disrupt central viability.