Order directly from

Glial Cell Line-Derived Neurotrophic Factor, or GDNF, is a neurotrophic factor that is closely related to other neurotrophic factors such as Neurturin, Persephin, and Artemin, by a common structural feature called the cysteine-knot. GDNF signals through a multicomponent system of receptors that includes receptors known as RET, and GFR alpha 1-4 to promote dopamine uptake, survival and differentiation of neurons. Rat GDNF is produced in E.coli. Recombinant rat GDNF is a non-glycosylated homodimer, containing two 135 amino acid chains, with a total molecular weight of 30.1 kDa.

Spec Container

Datasheet

Format

Each vial contains 10 ug of lyophilized protein. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.

E. coli. Endotoxin level as measured by LAL is <0.05ng/ug or <0.5EU/ug.

Purity

Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Application Notes

The activity is determined by the dose-dependent proliferation of C6 cells and is typically less than 0.1 ug/mL. Specific activity: 1 x 10e4 units/mg

Storage

Store at -20C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.