roGFP

Genetically encoded biosensors based on the redoxsensitive green fluorescent protein (roGFP) are a particularly powerful tool to monitor cellular redox changes. roGFP2 has two excitation peaks, at 405 nm and 488 nm, when monitoring fluorescence emission at 510 nm. The relative intensities of the two excitation peaks shift in an opposing direction upon roGFP2 reduction or oxidation.

roGFP measurements are usually performed on cell monolayers and benefit considerably from measurements from the bottom of the plate and from automated compound injection. The following features make the CLARIOstarthe ideal detection system for roGFP-based screening in live cells: