These structure-function studies suggest novel mechanisms for nmMLCK regulation, which may confirm MYLK as a candidate gene in inflammatory lung disease and advance knowledge of the genetic underpinning of lung-related health disparities.

Cow (Bovine) Myosin Light Chain Kinase (MYLK) interaction partners

Rabbit Myosin Light Chain Kinase (MYLK) interaction partners

evaluated the spatial arrangement of the catalytic/regulatory domain, three immunoglobulin-related motifs, one fibronectin-related motif, a repetitive, proline-rich segment, and, at the N-terminus, a unique F-actin-binding domain.

Mouse (Murine) Myosin Light Chain Kinase (MYLK) interaction partners

telokin may play a role in attenuating constitutive myosin light chain phosphorylation

MLCK is linked to timely blastocyst formation, though it is dispensable for oocyte meiotic maturation

a prominent effect of the multifunctional microRNA-155 in endothelial cells is modulation of phenotype through alterations in RhoA, myosin light chain kinase expression, and actin cytoskeleton organization

CArG element in intron 15 of the mylk1 gene is necessary for maximal expression of the 130-kDa smMLCK and that the 130-kDa smMLCK isoform is specifically required to regulate smooth muscle contractility

In immune-mediated inflammatory bowel disease, tight junction dysregulation and barrier loss is induced by MLCK, but at advanced stages, barrier loss is MLCK-independent.

Claudin-2 (show CLDN2 Proteins) assumes an important role in colorectal inflammation, and furthermore implicates the involvement of MLCK in colon inflammation.

Myosin Light Chain Kinase (MYLK) Protein Profile

Protein Summary

This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts.