Expression of IFN-γ, IL-1α, NGF-β and TNF-α during the development of cerebellar cortex of Western Anhui white gooseSONG Hai-Yan; LIU Zai-Qun & ZHENG Lei

Abstract

The strep avidin-biotin-peroxidase complex (SABC) immunohistochemical methods were applied to
investigate the localization and semi-quantitative distribution of IFN-γ, IL-1α, NGF-β and TNF-α-immunoreactive cells
in the cerebellar cortex of Western Anhui white goose at embryonic day 13, 19, 24, 28 (E13, E19, E24, E28) and
postnatal day 7, 15 (P7, P15). The possible roles of IFN-γ、IL-1α、NGF-β and TNF-α in the development of
cerebellar cortex were discussed. The results indicated that in the external granular layer, there were IFN-γ and TNF-α
positive cells at E13, E19, E24, E28, P7, IL-1α positive cells at E13, E19, E24, E28 and NGF-β positive cells at E13,
E19 , E24. The expression levels of these four cytokines all reached peaks at E19 of the six tested periods in this study. In
the Purkinje cell layer, there were IFN-γ, IL-1α and TNF-α positive cells at E13, E19, E24, E28, P7, P15 and NGF-β
positive cells at E13, E19, E24, E28, P7. In the internal granular layer, there were IFN-γ positive cells at E13, E19, E24,
E28, P7, P15, IL-1α and TNF-α positive cells at E13, E19, E24, E28, P7 and NGF-β positive cells at E13, E19, E24, E28.
These results showed that E19 might be the “critical stage” in the cerebellar cortex development of Western Anhui white
goose. IFN-γ, IL-1α and TNF-α might be synthesized by cerebellar cortex itself, and NGF-β could be transported from
regions which project to Purkinje cells. IFN-γ may interfer the transfer of granular cells, and NGF-β may have
neurotrophic functions that are beneficial to the growth and development of Purkinje cells.