Hello-
I am making DNA templates by PCR amplification to be used in
transcription. I have two major concerns. 1) The effects of UV on my
fragment. UV has been shown to have an effect on transcription. Hence, I
would like to minimize (or do away with!) such exposure. 2) High
efficiency recovery. Since these templates are a major part of my
research, I would like to make enough to last for awhile.
After running my PCR samples on an agarose gel, I have tried
electroelution, QIAgen, GeneClean, all with varying (and sometimes very
low) results.
Does anyone have any suggestions? If this question has already been
answered, I apologize for the repeat, but I'm new to this group. I tried
searching the archives but didn't come up with anything quite appropriate.
Thank you in advance for your assistance.
-Rachel Fish
rfish at ocf.berkeley.edu