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Samples prepared with this solution should be diluted with cold 50 mM perchloric acid prior to analysis. Discussion The retention times are such that assay of ascorbic acid can be performed at a sampling rate of 12-20 per hour depending on the chromatographic packing material chosen. Multivitamin formulations do not present any particular problems for iodimetric titration methods or LCEC except in those preparations containing ferrous sulfate. For these sample types, the chromatographic approach is superior.

This effect was due to the plug of high ionic strength that passed through the column. Samples prepared with the extracting solution were diluted prior to injection on the anion-exchange column. Cold 50 mM perchloric acid was found to be the ideal medium to inhibit the oxidation of ascorbic acid. Samples and standards were frequently diluted with this solution and stored refrigerated for several hours. Approximately 99% of the ascorbic acid remained after 12 hr when prepared in cold dilute perchloric acid.