Non-B DNA conformations are known to be associated with genetic mutations connected to over 70 serious diseases in human beings including polycystic kidney disease, adrenoleukodystrophy, follicular lymphomas, and spermatogenic failure, just to name a few.

Instead of investigating any potential problems which could be caused by injections of this new chemical compound, HPV vaccine proponents and government health officials chose to try and minimize the impact Dr. Lee’s discovery might make on HPV vaccination programs around the globe. Rather than conducting legitimate studies to determine the extent of potential risks, they chose to attack the messenger.

Helen Petousis-Harris PhD, the Director of Immunisation Research and Vaccinology Immunisation Advisory Centre at The University of Auckland, provided a prime example of these tactics in a presentation she gave at a public hearing on HPV vaccine safety in February 2014.

Following are two of the ‘concerns’ regarding Dr. Lee’s research she mentioned during this presentation:

The tests used were over sensitive, increasing the risk of amplifying irrelevant (junk) DNA

Confirmatory detection of human papillomavirus (HPV) L1 gene DNA sequences in the quadrivalent HPV vaccine Gardasil® based on virus-like particles production by recombinant expression of major capsid antigen L1 in yeast

Human papillomavirus (HPV) infection causes cervical cancer, a significant portion of anal, genital and oropharyngeal cancers, genital warts and recurrent respiratory papillomatosis. In June 2006, a prophylactic HPV vaccine (Gardasil®; Merck, NJ, USA) was licensed in the USA, with subsequent approval granted in the European Union. Gardasil® is a quadrivalent HPV protein-based vaccine containing genotype-specific L1 capsid proteins of HPV-16, HPV-18, HPV-6 and HPV-11 in the form of virus-like particles as the active ingredient, which are produced by a DNA recombinant technology in yeast. Recently Lee SH showed that Gardasil® contained fragments of HPV-11 or HPV-18 DNA, evidenced by nested PCR, of unknown significance [J Inorg Biochem. 2012 Dec;117:85-92]. We herein looked by optimized single PCR in different batches of Gardasil® from France for HPV L1 DNA using MY09/MY11 degenerate and nondegenerate primers, for HPV E2 and E6 DNA genes, and for contaminating Saccharomyces cerevisiae DNA. All amplified amplicons were sequenced and resulting FASTA sequences were analyzed by Genotyping software from NCBI. In-house quantitative single PCR using as external calibrator serial dilutions of HPV-16 DNA extracted from CaSki cell line allowed estimating the load of residual HPV DNA fragments in vaccine ampoules. Preliminary data showed the presence of contaminating HPV L1 DNA in all tested different batches of Gardasil® vaccine from France. Our observations confirm independently and extend the previous observations by Lee SH, without using conflicting nested PCR detection possibly subjected to contamination. Persistence in muscle tissue of residual HPV DNA fragments is uncertain after intramuscular injection, and requires further investigation for vaccination safety.

Co-authors

L. Belec1, H. Péré1, C. Fayard1.

1Microbiologie, Université Paris Descartes, Paris, France.

Not only has another independent laboratory confirmed the findings of Dr. Lee in every Gardasil sample tested from France, this lab used a different and less ‘sensitive’ methodology to arrive at the same conclusion regarding Gardasil recombinant (genetically engineered) HPV DNA protein fragment contamination.

It is interesting to note – both Dr. Lee and Prof. Bélec simply indicated the need for further investigation for vaccine safety.

The SaneVax team completely agrees – further investigation is necessary for vaccination safety.

Helen Petousis-Harris couldn’t have said it better when she quoted Carl Sagan at the end of her presentation.

Extraordinary claims require extraordinary evidence.

Marketing HPV vaccines as cancer preventatives is one of these extraordinary claims requiring extraordinary evidence.

Non-B DNA conformations are known to be associated with genetic mutations connected to over 70 serious diseases in human beings including polycystic kidney disease, adrenoleukodystrophy, follicular lymphomas, and spermatogenic failure, just to name a few.

Instead of investigating any potential problems which could be caused by injections of this new chemical compound, HPV vaccine proponents and government health officials chose to try and minimize the impact Dr. Lee’s discovery might make on HPV vaccination programs around the globe. Rather than conducting legitimate studies to determine the extent of potential risks, they chose to attack the messenger.

Helen Petousis-Harris PhD, the Director of Immunisation Research and Vaccinology Immunisation Advisory Centre at The University of Auckland, provided a prime example of these tactics in a presentation she gave at a public hearing on HPV vaccine safety in February 2014.

Following are two of the ‘concerns’ regarding Dr. Lee’s research she mentioned during this presentation:

The tests used were over sensitive, increasing the risk of amplifying irrelevant (junk) DNA

Confirmatory detection of human papillomavirus (HPV) L1 gene DNA sequences in the quadrivalent HPV vaccine Gardasil® based on virus-like particles production by recombinant expression of major capsid antigen L1 in yeast

Human papillomavirus (HPV) infection causes cervical cancer, a significant portion of anal, genital and oropharyngeal cancers, genital warts and recurrent respiratory papillomatosis. In June 2006, a prophylactic HPV vaccine (Gardasil®; Merck, NJ, USA) was licensed in the USA, with subsequent approval granted in the European Union. Gardasil® is a quadrivalent HPV protein-based vaccine containing genotype-specific L1 capsid proteins of HPV-16, HPV-18, HPV-6 and HPV-11 in the form of virus-like particles as the active ingredient, which are produced by a DNA recombinant technology in yeast. Recently Lee SH showed that Gardasil® contained fragments of HPV-11 or HPV-18 DNA, evidenced by nested PCR, of unknown significance [J Inorg Biochem. 2012 Dec;117:85-92]. We herein looked by optimized single PCR in different batches of Gardasil® from France for HPV L1 DNA using MY09/MY11 degenerate and nondegenerate primers, for HPV E2 and E6 DNA genes, and for contaminating Saccharomyces cerevisiae DNA. All amplified amplicons were sequenced and resulting FASTA sequences were analyzed by Genotyping software from NCBI. In-house quantitative single PCR using as external calibrator serial dilutions of HPV-16 DNA extracted from CaSki cell line allowed estimating the load of residual HPV DNA fragments in vaccine ampoules. Preliminary data showed the presence of contaminating HPV L1 DNA in all tested different batches of Gardasil® vaccine from France. Our observations confirm independently and extend the previous observations by Lee SH, without using conflicting nested PCR detection possibly subjected to contamination. Persistence in muscle tissue of residual HPV DNA fragments is uncertain after intramuscular injection, and requires further investigation for vaccination safety.

Co-authors

L. Belec1, H. Péré1, C. Fayard1.

1Microbiologie, Université Paris Descartes, Paris, France.

Not only has another independent laboratory confirmed the findings of Dr. Lee in every Gardasil sample tested from France, this lab used a different and less ‘sensitive’ methodology to arrive at the same conclusion regarding Gardasil recombinant (genetically engineered) HPV DNA protein fragment contamination.

It is interesting to note – both Dr. Lee and Prof. Bélec simply indicated the need for further investigation for vaccine safety.

The SaneVax team completely agrees – further investigation is necessary for vaccination safety.

Helen Petousis-Harris couldn’t have said it better when she quoted Carl Sagan at the end of her presentation.

Extraordinary claims require extraordinary evidence.

Marketing HPV vaccines as cancer preventatives is one of these extraordinary claims requiring extraordinary evidence.

No surprise there. Do you know much of new drug development is done in 3rd world countries? I have a couple of pharmacists in my family, and they had to go to a third world country for several months as part of their training……how to make drugs, mixtures, etc………I don’t know what country they use now, but 15 years ago, it was the Philippines.
The so-called myth of the millions spent in R & D of new drugs is another scam….it is done offshore, by pharmacy students. The lies are everywhere, and all I can say in light of the experiences of my people, medical doctors, etc…….stay the hell away from them.
I avoid doctors like the plague. I have no respect for most of them. The few I do like and trust I am open and firm with them……I make the decisions, not them. I research my own problems, and discuss from that vantage point. It pays good dividends .
They also treat me with respect.
They are not people to look up to, the more who do that the bigger their egos……
I have been known to walk out of doctor’s offices who are jerks. I suggest that others do the same.