After retrieving each of the artifacts, Desmond would store them on a set of shelves within the Sanctuary, in which he and his fellow Assassins were hiding, and they could be seen situated to the left of his laptop.

Contents

Artifact locations

Maria's Feather Chest

Upon exiting the Sanctuary, Desmond could find the chest by turning around and freerunning up a wall towards a round window. After this, there was a lift nearby that would send him to the top of the roof, with the feather chest being located to the left of the landing spot, inside a cannonball crater.

Ezio's Belt

The second artifact was the belt worn by the Master Assassin Ezio Auditore, and could be located at the very front of the city, opposite of the Sanctuary, where the brothel would stand, if you're facing the front gate its in a puddle to the right, on the elevated part.

Claudia's Record Book

When Desmond exited the Sanctuary, he could obtain the book by turning around and re-entering Mario's study, though he had to be careful not to go too far into the room, or he would return to the Sanctuary. The book was to the right side of the doorway, along the wall.

Mario's Sword

The sword that Mario Auditore used as his personal blade could be obtained by Desmond.

Upon exiting the Sanctuary, Desmond could find the blade by going to the front of the villa. From there, he would need to head down the stairs towards the training ring and jump over the ledges directly in front of him. After this, he would carry onwards until he reached a street with trashcans to his left and a white car to his right. Following this, Desmond would then head down the alley that the car was in to the right, making his way towards a tree. The sword would be sticking out of a stone directly next to the tree, to which he could then pull the artifact from the ground.

Trivia

The shelf where the artifacts are stored was empty right before Desmond and the others left for the Colosseum, regardless of how many Desmond found.

Despite the fact that Ezio received his new robes after his arrival in Rome, the belt found bears the Roman variation of the Assassin insignia.

The achievement "Dust to Dust" required that Desmond find at least one of the artifacts.

The items did not appear until Desmond had rerouted the power to the Villa and entered the Animus.

The location of Mario's sword bears some similarities to The Sword in the Stone, since both Mario's sword and Excalibur were pulled from stones by descendants of former rulers.