Recently, we demonstrated that the hepatoportal NaィイD1+ィエD1-sensitive mechanism plays a significant role in maintaining body fluid homeostasis by controlling renal excretory and jejunal absorptive functions. The afferent pathway of the hepatic body fluid control system is the periarterial hepatic nerve and efferent pathways are the renal sympathetic nerve and cholinergic fibers to the jejunum. However the sensor mechanisms and central pathways are still unknown. Accordingly, the purposes of the present study was to examine these two points and the following conclusions were obtained:(1) The hepatoportal NaィイD1+ィエD1-receptive signals projects to the brain stem, hypothalamus, and forebrain structures involved in autonomic function and osmoregulation, then activate the fluid-regulatory mechanism. However, the hepatoportal osmoreceptive signals do not activate these central structures.(2) The hepatoportal NaィイD1+ィエD1 receptor senses the NaィイD1+ィエD1 concentration via the bumetanide-sensitiv
… Moree NaィイD1+ィエD1 KィイD1+ィエD1 2ClィイD1-ィエD1 cotransporter.(3) The hepatoportal bumetanide-sensitive NaィイD1+ィエD1 KィイD1+ィエD1 2ClィイD1-ィエD1 cotransporter also participate in the KィイD1+ィエD1-sensitive mechanism. When this mechanism is stimulated, kaliuresis occurs via the periarterial hepatic nervous plexus. Thus, the hepatoportal bumetanide-sensitive KィイD1+ィエD1-sensor mechanism may play an important role in regulating extracellular KィイD1+ィエD1 homeostasis.The hepatoportal NaィイD1+ィエD1- and KィイD1+ィエD1-sensor mechanisms would be triggered by an increase in the portal venous NaィイD1+ィエD1 and KィイD1+ィエD1 concentrations in advance of changes in the systemic blood NaィイD1+ィエD1 and KィイD1+ィエD1 concentrations and reflexively regulate NaィイD1+ィエD1 and KィイD1+ィエD1 excretion. The important feature of this system is that the portal venous blood flow is 20〜25 % of cardiac output and the concentration of ions and the changes therein are therefore 4-5-times greater than in the systemic circulation. If the purpose of this system is to regulate the systemic blood NaィイD1+ィエD1 and KィイD1+ィエD1 concentrations, but not the portal venous NaィイD1+ィエD1 and KィイD1+ィエD1 concentrations, it would be operated by predicting the systemic blood concentrations from the portal venous concentrations and such prediction can result in errors of regulation. This control error would be corrected by other negative feedback systems.以上のように,門脈―肝臓領域Na^+受容器の求心路,遠心路,効果器等についての報告を行ってきたが,その受容機構および中枢機構については不明である。したがって,本研究はこの2点を明らかにするために行い,以下の結果を得た。1.肝臓のNa^+感受性神経は延髄および視床下部の自律神経および体液向上性維持に関与する中枢に投射する。2.門脈―肝臓領域Na^+受容機構にブメタナイド感受性Na^+K^+2Cl^-共輸送体が関与している。また,ブメタナイド感受性Na^+K^+2Cl^-共輸送体は,門脈―肝臓領域のK^+濃度感知にも関わっており,肝門脈内のK^+濃度が増加すると腎臓からのK^+排泄が増加する。 Less