5′-Amino modifiers are phosphoramidites which are used to produce a functional amine group on the 5′-terminus of oligonucleotides. The modifiers incorporate a C6 spacer with a primary amino group which can be used for subsequent conjugation to a vast majority of applications, such as dyes or labesls. The MMT protecting group can be removed on the DNA synthesizer by DCA treatment (usually 3% DCA/DCM). For cartridge purification, the final cleavage of the MMT group can be accomplished using a TFA solution (usually 2.5% TFA).

Technical Information

Molecular Formula: C35H48N3O3P

Molecular Weight: 589.76 g/mol

Specification:

HPLC – Identity is confirmed by comparison with a reference sample, Minimum purity

NMR – Purity is determined by 31P NMR and isomeric purity is veified by 1H NMR