Background of HDAC8 antibody

Regulation of gene expression is mediated by several mechanisms such as DNA methylation, ATP-dependent chromatin remodeling, and post translational modifications of histones, which include the dynamic acetylation and deacetylation of epsilon-amino groups of lysine residues present in the tail of core histones. The enzymes responsible for reversible acetylation/deacetylation processes are histone acetyltransferases(HATs) and histone deacetylases (HDACs), respectively. HATs act as transcriptional coactivators, and HDACs are part of transcriptional corepressor complexes. Mammalian HDACs can be divided into three classes according to sequence homology. Class I consists of the yeast Rpd3-like proteins (HDAC1, HDAC2, HDAC3, and HDAC8). Class II consists of the yeast Hda1-like proteins (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9, and HDAC10). Class III comprises the yeast Sir2-like proteins. Class I HDACs are ubiquitously expressed, and most class II HDACs are tissue-specific. Class II HDACs have been implicated in the regulation of muscle differentiation. Interaction of HDAC4, 5, and 7 with members of the MEF2 family of transcription factors represses their transcriptional activity and prevents myogenesis. The deacetylase activity of class II HDACs is regulated by subcellular localization. The HDAC8 gene encodes for a 377 amino acid protein with a molecular weight of 43 kDa and is localized within the nucleus. HDAC8 mRNA is expressed in heart, lung, kidney, and pancreas as well as in several cell lines derived from cancer tissues.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the HDAC8 polyclonal antibody ( Cat # PAB2336 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunohistochemical analysis of Histone Deacetylase 8 (pS39) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Histone Deacetylase 8 (pS39) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).