Metadata

Citable URI

As published

DOI

10.1128/AEM.00332-06

Abstract

Harmful algal blooms (HABs) are a serious threat to coastal resources, causing a variety of impacts on public health, regional economies, and ecosystems. Plankton analysis is a valuable component of many HAB monitoring and research programs, but the diversity of plankton poses a problem in discriminating toxic from nontoxic species using conventional detection methods. Here we describe a sensitive and specific sandwich
hybridization assay that combines fiber-optic microarrays with oligonucleotide probes to detect and enumerate the HAB species Alexandrium fundyense, Alexandrium ostenfeldii, and Pseudo-nitzschia australis. Microarrays were prepared by loading oligonucleotide probe-coupled microspheres (diameter, 3 μm) onto the distal ends
of chemically etched imaging fiber bundles. Hybridization of target rRNA from HAB cells to immobilized
probes on the microspheres was visualized using Cy3-labeled secondary probes in a sandwich-type assay
format. We applied these microarrays to the detection and enumeration of HAB cells in both cultured and field
samples. Our study demonstrated a detection limit of approximately 5 cells for all three target organisms
within 45 min, without a separate amplification step, in both sample types. We also developed a multiplexed
microarray to detect the three HAB species simultaneously, which successfully detected the target organisms,
alone and in combination, without cross-reactivity. Our study suggests that fiber-optic microarrays can be used
for rapid and sensitive detection and potential enumeration of HAB species in the environment.

Under the auspices of the International Association for Physical Sciences of the Ocean (IAPSO) a committee was established to
identify the oceanographic and geophysical requirements for fixing Tide Gauge Bench Maries ...

A set of terms recommended for use in facilitating communication in biological nomenclature is presented as a table showing broadly equivalent terms used in the traditional Codes of nomenclature. These terms are intended ...

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