Tetracycline-Inducible shRNA- Tet-tTS Cell Lines

Accessory cell lines for Clontech’s Tet-inducible shRNA systems.

Tet-tTS cell lines have been clonally selected to express the Tetracycline transcriptional silencer, or tTS, and greatly simplify the task of creating a tetracycline inducible shRNA cell line, as they are ready-made hosts for our Knockout Tet RNAi Systems H and P.

How tTS Works…

In the absence of doxycycline (Dox), tTS binds tightly to Tet operator sequences (tetO) in the promoter of Clontech’s inducible shRNA vectors. The KRAB domain of tTS then acts as a potent suppressor of shRNA transcription. When Dox is added to the culture medium, tTS dissociates from tetO to allow full promoter activity and high-level shRNA expression.

Features

Applications

Inducible shRNA for gene knockdown in mammalian cells

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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