Goal: How can we help solve theGoal: How can we help solve theworldworld’’s energy problems??s energy problems??Find a way to maximize the production of hydrocarbons Find a way to maximize the harvest (yield) of hydrocarbons

Novelty of Our SystemNovelty of Our System…Why AlgaeWhy AlgaeE coli Must be forced to produce fuels Algae Optimized for the production of fuel Yeast Produces ethanol (inferior) Problem: export is extremely difficult

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Novelty of Our SystemNovelty of Our System…Metabolic engineeringMetabolic engineeringGoal: force hydrocarbons to be secreted from the algae How? ◦Unblock the path by minimizing cell wall ◦Increase the production of hydrocarbons Ramp up hydrocarbon pathway Cut off carbon diversion to other pathways

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Which species of algae?Which species of algae?C. reinhardtii Use as a model for our system Is already sequenced Successful homologous recombination B. braunii Our ideal species Has most productive fuel pathways Produces hydrocarbons vs fatty acids Problem: has not been sequenced Photo removed due to copyright restrictions.

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