SepQuant dropletProbe Features Overview

Developed in conjunction with Oak Ridge National Laboratory, the SepQuant dropletProbe allows liquid surface extraction of analytes on surfaces and further analysis in an HPLC-MS detector. The technique allows a powerful alternative to Quantitative Whole Body Autoradiography (QWBA) and maximises the use of advanced mass spectrometry platforms for analytes detection.

Feature 2

Schematic of the surface sampling process including (a)determining syringe-to-surface distance, (b) the positioning of thesyringe needle 100-300 μm above a defined surface spot, (c)dispensing a discrete volume of extraction solvent onto a surfacecreating a liquid junction between the needle and the surface,(d) dissolution of the analyte in the extraction solvent, e) the liquidis drawn back into the syringe needle after a predefined extractiontime, and (f) loaded into the sample loop followed by (g) injection fora consecutive HPLC-MS/MS analysis. Steps (c)-(e) may be repeatedto improve extraction efficiency.

Feature 3

Extraction liquid dispensed by the needle in locked positionon (a) a tissue biopsy sample and on (b) a brain thin tissue section.Zoomed in view in inset in (b) shows of liquid microjunction betweenthe tip of the needle and the sample.

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HTX Imaging is dedicated to the ongoing advancement of molecular imaging technologies, with a primary focus on Mass Spectrometry imaging.