Case Study B2 - Resolution

ABO grouping*

* Reverse group with 4 drops of patient plasma and 1 drop of red cells, incubated at RT.

ABO

anti-A

anti-B

A1 cells

B cells

Interpretation

RT

IS

0

4+

0

0

invalid**

Inc.

15'

0

4+

1+

0

invalid**

RT - room temperature IS- immediate spin

** according to transfusion service SOP

The following enhancement was done:

* Reverse group with 2 drops of patient plasma and 1 drop of red cells, incubated at 4° C for 15 minutes

ABO grouping

anti-A

anti--B

A1cells

B cells

Interpretation

RT

IS

0

4+

0

0

invalid

4° C

15'

0

4+

2+

0

B

Controls - ABO grouping

Cells*

RT - IS

4° C - 15'

Screen Cell 1

0

0

Screen Cell 2

0

0

Screen Cell 3

0

0

Autocontrol

0

0

* The autocontrol can detect if a cold autoantibody such as autoanti-I is present (which would react with both A1 and B cells). The group O screen cells is included to detect possible cold-reactive alloantibodies such as anti-N, -P1, and -Lea (whose corresponding antigens may be present on either or both of the A1 and B cells).