Fig. 1

Synthetic lethal short hairpin RNA (shRNA) screening shows that ring finger protein 183 (RNF183) confers resistance to trametinib treatment in colorectal cancer (CRC) cells. a HT29 cells were infected with a pooled shRNA library, and the puromycin-selected stable cells were treated with dimethyl sulfoxide (DMSO) or 20 nmol/L trametinib for 8 days. Then, shRNA sequences were amplified by PCR, and next-generation sequencing was conducted to calculate their abundance. b The abundance of RNF183 shRNA decreased in cells treated with trametinib compared with those treated with DMSO. T/D ratio of abundance, the ratio of abundance in the trametinib group to abundance in the DMSO group