应用

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用

Ab评论

说明

ICC

IHC-Fr

IHC-P

应用说明ELISA: Use at an assay dependent concentration.
ICC: Use at an assay dependent concentration.
IHC-P: Use at an assay dependent dilution (PMID 18457899).
IHC-Fr: Use at an assay dependent concentration.

The recommended dilution can be between 1:2,000-1:5,000.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

靶标

相关性Noradrenaline (NA) is a catecholamine neurotransmitter at postganglionic sympathetic fibres, produced following a series of steps from the essential amino acid phenylalanine. Terminal postganglionic sympathetic fibres have varicosities (where NA is synthesised and stored) which form synapses with the effector organ. The adrenal medulla is a major source of catecholamines (such as NA and adrenaline), however, most of the NA produced in the adrenal medulla is converted into adrenaline. NA is key in the fight-or-flight response and also directly increases heart rate, energy release from fat, and muscle readiness.

Cells were transfected with conjugated Noradrenaline before being fixed and permeabilized with 0.1% Triton X-100 for 20 minutes. Samples were incubated with primary antibody at 1/500 dilution. A fluorescence-conjugated anti-rabbit IgG was used as the secondary antibody.

For ab6454, it looks like the antibody was tested with glutaraldehyde-perfused tissue sections. There is a link to "Immunohisto and cytochemistry" protocol at the very bottom of the datasheet- Read More

Thank you for your enquiry.
The FAQ you noticed is from Thursday 25-November-2004 at which time the antibody had not been tested in IHC-P.
We since have data from a publication that came to our attention in Sept 2008. PMID 18457899. Thi...

Thank you for your enquiry.
Regarding "using protein in the phenol-ethanol supernatant for ELISA", would you first precipitate the protein? In either case, I'm not sure if the protein would be recognized by the antibodies since we have not tested t...

Thank you for your patience and forwarding your detailed protocol on-line. We are very sorry to hear that you are having some problem with this antibody.
Looking through your email, I can understand that you have fixed the tissue sections in parafor...