Continuation of the Mutagenesis Reaction

On Tuesday, we ran the gel for last week's PCR products. The high concentration of 1:20 template DNA, and a low concentration of 1:200 were prepared with water for each the upstream and downstream fragments. Our nomenclature used is three-part, and outlined below.

1.)Primer configuration

N = no primers used

P = outside primers used

2.)Template DNA concentration

+ = 1:20

- = 1:200

3.)Annealing temperature for PCR, in °C

Our positive control was standard template DNA and associated primers from lab stock