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Integrative workshop WP 5 Chemosynthetic ecosystems Ghent University, Belgium November 2011 Integration biodiversity data from microbiota to megafauna from different seeps along the European margins

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1) What are major scales of turnover in biodiversity? Do we observe the largest turnover at micro (within habitats), meso (between habitats), macro (between seeps or MV's from the same region) or mega scale (between regions) for different taxa/size groups/functional groups (chemo vs non chemosynthetic species)? HH if it becomes apparent – also see Cordes paper. 2) What is alpha, beta and gamma diversity for the different regions, and can we identify regional hot spots of biodiversity? 3) What is the degree of connectivity between different seeps at different scales? Can we identify true endemics and cosmopolitan species/taxa? 4) What is relationship between biodiversity and seep intensity (or productivity)? Are the most productive sites, the least diverse? 5) HH 6) Island Theory Research Questions

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Agree on measures for turnover and graphical representation -BrayCurtis and Jaccard – (dis)Similarity indices -Accumulation curves -Variation partitioning (Veech & Crist 2007) - Color-coded matrix - BetaDisp/permdisp Agree on measures for alpha and graphical representation Agree on measures for gamma and graphical representation -Rarefraction (non-parametric, EstimateS) -RTS curves -OTU counts for microbiota 1) What are major scales of turnover in biodiversity? Do we observe the largest turnover at micro (within habitats), meso (between habitats), macro (between seeps or MV's from the same region) or mega scale (between regions) for different taxa/size groups/functional groups (chemo vs non chemosynthetic species)? 2) What is alpha, beta and gamma diversity for the different regions, and can we identify regional hot spots of biodiversity? -Rarefraction (non-parametric) -RTS curves -OTU counts for microbiota

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What is the degree of connectivity between different seeps at different scales? Can we identify true endemics and cosmopolitan species/taxa? Venn diagram of the 3 regions Network analysis (graphical) Research Question 3