Quality of RNA by spectrophotometer - (Jun/27/2005 )

The A260/A280 ratio of my RNA purification is often >2 sometimes even >3. Does anyone know the cause of this and how to ommit it?

The RNA is purifyied from barley plants with the RNAeasy kit from qiagen with an on-collum DNase treatment. The RNA is going to be used for real time RT-PCR. Is the high ratio a problem for this application?