Figure 2.

Analysis of variation of identified SNPs via high resolution melting (HRM) curves
generated on 8 cultivars used in this study. HRM derivative plots, -(d/dT) fluorescence as a function of temperature, of several
primer sets when analyzing 8 sweet cherry cultivars representing the common patterns
observed during analysis. Comparisons outside one frame are not meaningful and the
frames are not to scale with each other as the curve shape is the focus. A-C are from primers amplifying a region expected to contain 1 putative SNP while D contains 2, E has 3, and F contains 5. A. Primer set 121 produces a single curve pattern denoted by an arrow representing
either a homozygous locus across all 8 cultivars tested or a heterozygous locus shared
by all 8 tested cultivars. B. Primer set 100 has three distinct curve patterns highlighted as 1, 2 and 3 representing
three allelic forms at the sampled locus. C. Primer set 115 has an indiscernible pattern. D-F. Each demonstrates variation in the population; however, the more SNPs present in
the amplified region the smaller the differences among the melt curves.