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Abstract

We report the design and implementation of a multispectral imaging Fourier transform endospectroscopy (IFTES) system. The IFTES system employs a flexible fiber bundle catheter coupled to a home-built imaging Fourier transform spectroscope. The instrument enables the performance of non- or minimally invasive subsurface imaging and multispectral imaging at the cellular level in vivo and in situ. A maximum spectral resolution of 0.2 nm at 632.8 nm and a lateral resolution of 4.4 μm were proved. Preliminary results of a standard resolution target, ex-vivo small animal tissue, single wavelength laser, fluorescence solution, in-vivo mouse skin, microspheres mixture, and in-vivo transgenic mouse brain were given to demonstrate the potential of the technique.

Spectral imaging of a He-Ne Laser. (a) Interferogram frame of the fiber bundle illuminated by a He-Ne laser (false color by ImageJ software; the same below). (b) Enlarged detail of the blue rectangle in (a). (c) Spectrum of the red pixel pointed out by the blue arrow in (b). Scale bar is 100 μm.

Spectral imaging results from C57 mouse skin in situ. (a) Full FOV image acquired by the fiber bundle in the living C57 mouse. Red rectangle represents ROI range. Scale bar is 50 μm. (b) Emission spectra of the bright spot pointed out by the red cross in (a) by the IFTES system and commercial spectroscopy are plotted as blue and red lines, respectively.