Marsha
I use 10% serum of the species from which the 2nd is made, 5% RIA grade
BSA in buffer (TRIS or PBS). Depending on the specific antibody, I may
include 0.1% Triton X-100 or Tween 20. The 10% normal serum is probably
overkill since when I double label in a cocktail the sera are at 5%.
I think this is the heart of the basic recipe. Variants derive from what
lab you grew up in and what specifics you're dealing with. Or so I think.
Cheers
John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL 36849
On Tue, 6 Apr 1999, marsha r price wrote:
> Hello Histonetters,
> I would like a recipe for making my own Primary Antibody diluent
> (Monoclonal & Polyclonal)
> Thank You.
> Marsha Price HT (ASCP), QIHC (ASCP)
>