Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.

Form Supplied

Liquid

Concentration

1.0
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Purified by antigen-affinity chromatography.

Purification Note

This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed agains

Specifications: PAK1 + PAK2 + PAK3 (phospho Thr423) antibody

Full Name

p21 protein (Cdc42/Rac)-activated kinase 1/2/3

Background

The p21-activated kinases (PAKs) are a family of multifunctional serine/threonine kinases involved in a variety of cell functions including stress response, apoptosis and regulation of cell motility and tumor metastasis. Mammalian PAKs are called 1, 2, 3 or a, g, b respectively. PAKs are part of a large family of kinases where the catalytic domain of the kinase is related to Ste20 kinase of S. cerevisiae. Pak activity is regulated by specific binding of GTP-bound Rac and cdc42 GTPases and also by sphingosine and related lipids. PAK1 activation is induced by a variety of growth factors and G-protein-coupled receptors, Fc receptors, and integrins. This antibody is specific for the phosphorylated form of PAK 1+2+3. The selected peptide sequence used to generate the polyclonal antibody is common to all human PAKs.

Storage Conditions: PAK1 + PAK2 + PAK3 (phospho Thr423) antibody

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing.

Notes

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

PAK1 + PAK2 + PAK3 (phospho Thr423) antibody validated data

GTX22477 WB Image

Western blot probing with PAK1 antibody (GTX22477). The major band is at 65 kDa and weaker bands can be detected at 90 and 100 kDa. This picture was kindly submitted by Sue Thomas as part of her review.

Western blot probing with PAK1 antibody (GTX22477). The major band is at 65 kDa and weaker bands can be detected at 90 and 100 kDa. This picture was kindly submitted by Sue Thomas as part of her review.