RT Journal
A1 Mitchell J
T1 A new method for fixation of paraffin sections
JF Journal of the American Medical Association
JO Journal of the American Medical Association
YR 1919
FD January 25
VO 72
IS 4
SP 275
OP 275
DO 10.1001/jama.1919.26110040002016a
UL http://dx.doi.org/10.1001/jama.1919.26110040002016a
AB A new method for the fixation of paraffin sections to slides, doing away with the troublesome albumin fixative, has been devised by Land1 which depends on the well known principle that most colloidal substances when treated with a solution of a salt of chromium, exposed to light and dried, become insoluble in water. In his paper Land described his technic as follows:A 1 per cent. solution of acacia that has been made slightly yellow by the addition of a few drops of 0.2 per cent. potassium bichromate is allowed to flow over a thoroughly clean slide. The paraffin ribbon is then placed on this slide and exposed to light until dry.In working with this method I found that the paraffin sections were invariably fixed to the slide after a few hours' exposure to light. The fixative used in this manner, however, had the disadvantage of taking up