I've heard that the fixation step (with 10% acetic acid & 10% methanol) can be
skipped without much difference in the result during the normal Sanger's DNA
sequencing procedures. Does anyone have any experience or opinion about this?
Tsute Chen E-mail: tsute at microbio.umass.edu
Department of Microbiology
University of Massachusetts
Box 35720
Amherst, MA 01003-5720