Received May 17, 2017; Revision received June 13, 2017
Aminoglycoside antibiotics affect protein translation fidelity and lead
to protein aggregation and an increase in intracellular oxidative
stress level as well. The overexpression of the chaperonin GroEL/GroES
system promotes short-term tolerance to aminoglycosides in
Escherichia coli. Here, we demonstrated that the coexpression of
prefoldin or Hsp60 originating from the hyperthermophilic archaeon
Pyrococcus furiosus in E. coli cells can rescue cell
growth and inhibit protein aggregation induced by streptomycin
exposure. The results of our study show that hyperthermophilic
chaperones endow E. coli with a higher tolerance to streptomycin
than the GroEL/GroES system, and that they exert better effects on the
reduction of intracellular protein misfolding, indicating that these
chaperones have unique features and functions.
KEY WORDS: aminoglycoside, protein mistranslation, Escherichia
coli, hyperthermophilic archaeon, Pyrococcus furiosus,
chaperone