RE: some general questions on the TUNEL assay

From:

=?iso-8859-1?Q?Martin_Beno=EEt?=

Could you explain me the TUNEL method ?
Benoit MARTIN
Bruxelles
> -----Message d'origine-----
> De: Amanda Gifford [SMTP:agifford@ctl.net]
> Date: mercredi 18 septembre 2002 20:29
> À: histonet@pathology.swmed.edu
> Objet: RE: some general questions on the TUNEL assay
>
>
>
> I have gotten consistent results with TUNEL without working DNase
> free. I have never tried it on decalcified tissue, but I'm not aware of
> problems with EDTA. High concentrations of HCl would affect the DNA, but
> I doubt the concentration in the decalcifying solution would affect the
> results of TUNEL. Again though, I have not tried it.
>
> Amanda Gifford, HTL
> CTL Immunotherapies
> agifford@ctl.net
>