There are 3-9 nuclear chain fibers per muscle spindle that are half the size of the nuclear bag fibers. Their nuclei are aligned in a chain and they excite the secondary nerve. They are static while the nuclear bag fibers are dynamic in comparison. The name "nuclear chain" refers to the structure of the central region of the fiber, where the sensory axons wrap around the intrafusal fibers.

The secondary nerve association involves an efferent and afferent pathway that measure the stress and strain placed on the muscle (usually the extrafusal fibers connected from the muscle portion to a bone). The afferent pathway resembles a spring wrapping around the nuclear chain fiber and connecting to one of its ends away from the bone. Again, depending on the stress and strain the muscles sustains, this afferent and efferent coordination will measure the "stretch of the spring" and communicate the results to the central nervous system.

Also note that a similar structure attaching one end to muscle and the other end to a tendon is known as a Golgi tendon. However, golgi tendons differ from nuclear chain and nuclear bag fibers in that they are considered in series rather than in parallel to the muscle fibers.

Innervation

As intrafusal muscle fibers, nuclear chain fibers both send afferent innvervation and receive efferent innervation. The afferent innervation is via Group II and Ia neruons. These project to the nucleus proprius in the dorsal horn of the spinal cord. Efferent innervation is via static γ nerons. Stimulation of γ neurons causes the nuclear chain to shorten along with the extrafusal muscle fibers. This shortening allows the nuclear chain fiber to be sensitive to changes in length while its corresponding muscle is flexed.