Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Dukes' type C, grade III, colorectal adenocarcinoma

Age

53 years

Gender

female

Ethnicity

Caucasian

Storage Conditions

liquid nitrogen vapor phase

Clinical Data

female

53 years

Although the patient from which this cell was derived was blood type B, the cell line does not react with anti type B antibody despite the presence of normal levels of alpha1,3-galactosyltransferase activity.

Caucasian

Antigen Expression

blood type B; Rh+

Oncogene

myc +; ras +; fos +; sis +; p53 -; myb -; abl -; ros -; src -

Genes Expressed

carcinoembryonic antigen (CEA) 362 ng/106 cells/10 days; keratin

Tumorigenic

No

Effects

No, in immunosuppressed mice

Yes, in semisolid medium

Comments

Although the patient from which this cell was derived was blood type B, the cell line does not react with anti type B antibody despite the presence of normal levels of alpha1,3-galactosyltransferase activity.

N-myc, K-ras, N-ras and myb oncogene expression were not detected.

The cells are positive for keratin by immunoperoxidase staining.

The line is positive for expression of c-myc, H-ras, sis and fos oncogenes.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)

Subculturing

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.