B220/CD45R Antibody (20.96) Summary

Immunogen

Ovine thymocytes

Specificity

NB100-65367 recognizes the ovine CD45R cell surface antigen, a 220kD glycoprotein, which is expressed by all B lymphocytes and a subset of T lymphocytes. In immunohistochemical staining the antibody labels all lymphocytes within the B cell follicles as well as scattered cells in interfollicular areas. *This clone has been reported to react with paraffin-embedded material following alcohol fixation. Please refer to ref. Sainte Marie, et al for alcohol fixation protocol.

Isotype

IgG1

Clonality

Monoclonal

Host

Mouse

Gene

PTPRC

Purity

Protein G purified

Innovator's Reward

Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Buffer

PBS (pH 7.4)

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Purity

Protein G purified

Alternate Names for B220/CD45R Antibody (20.96)

B220

CD45 antigen

CD45R

EC 3.1.3.48

L-CA

LY5

protein tyrosine phosphatase, receptor type, C

receptor-type tyrosine-protein phosphatase C

T200 glycoprotein

T200 leukocyte common antigen

T200receptor type, c polypeptide

Background

CD45R is a member of the protein tyrosine phosphatase (PTP) family and a major cell surface glycoprotein. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45R represents a restricted form of the CD45 family which primarily recognizes only cells of B lineage from proB cell through mature B lymphocytes and, prior to the availability of anti CD19 MAbs, was commonly used as a pan B cell marker. It also reacts with certain activated T cells, as well as non MHC restricted lytically active lymphokine activated killer (LAK) cells. CD45R contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T and B cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. CD45R also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Contact Information

Product PDFs

Calculators

Concentration Calculator

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

Volume

=

Mass

÷

Concentration

Review this Product

Be the first to review our B220/CD45R Antibody (20.96) and receive a gift card or discount.