Penta·His Antibody, BSA-free

Monoclonal mouse Anti·His Antibodies are used to detect recombinant proteins carrying His tags. Each of the three variants detects a different His tag epitope. Like all QIAGEN mouse monoclonal antibodies, they are prepared under serum-free conditions, which guarantees the absence of viruses, mycoplasma, and contaminating immunoglobulins.

The Penta·His Antibody, BSA-free is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Sensitivity of QIAexpress Anti·His Antibodies.

Detection of 6xHis-tagged proteins (A–H, see table "Proteins tested with QIAexpress Anti·His Antibodies") with QIAexpress Anti·His Antibodies. Indicated amounts of pure 6xHis-tagged protein were applied to a nitrocellulose membrane, and detection was carried out with the Anti·His primary antibody indicated diluted 1/2000, followed by chromogenic detection with AP-conjugated rabbit anti-mouse IgG and NBT/BCIP.

* Detection using chromogenic substrate. Sensitivity may vary with different proteins.

Many different proteins have been detected, via their His tags, using the QIAexpress Anti·His Antibodies (see table). For the sensitivity of the three Anti·His Antibodies in detecting this panel of proteins see the figure Sensitivity of QIAexpress Anti·His Antibodies)

All QIAexpress mouse monoclonal antibodies are produced under conditions that guarantee the highest purity and activity. Hybridoma cell culture is carried out under serum-free conditions, ensuring that the preparations are free of viruses, mycoplasma, and contaminating immunoglobulins. Purification by adsorption chromatography is performed entirely at physiological pH, yielding preparations with the highest proportion of native antibody. See figure Immunohistochemistry with Penta·His Antibodies.

Applications

QIAexpress Detection Systems allow efficient detection of His-tagged proteins and are ideal for many applications, including: