Summary

Secondary Antibodies

Negative Isotype Controls

Useful Reagents

Positive Controls

Histology Controls

More Images

References

Reviews

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Rabbit anti ECSIT (C-Terminal) antibody recognizes Ecsit (Evolutionary conserved signalling intermediate in Toll pathway), an adaptor protein of the Toll-like and IL-1 receptor signalling pathway. It is involved in the activation of NF-kappa-B, acting as a regulator of MEKK-1 processing by promoting its proteolytic activation. Ecsit is also involved in the bone morphogenetic protein signalling pathway and is required for normal embryonic development.

Intended Use

Target Species

Human

Species Cross-Reactivity

Target Species

Cross Reactivity

Mouse

N.B. Antibody reactivity and working conditions may vary between species.

A peptide corresponding to 14 amino acids near the carboxy terminus of human ECSIT.

Purity

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

IgG concentration 1.0mg/ml

Reagents In The Kit

Preparing The Antibody

Test Principle

Buffer Solution

Phosphate buffered saline

Phosphate buffered saline

Storage

Store at +4oC or at -20oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Store at +4oC or at -20oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name

Yes

No

Min Dilution

Max Dilution

Western Blotting

0.5

1.0ug/ml

Immunohistology - Paraffin(1)

2ug/ml

(1)

This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Application Name

Yes

No

Min Dilution

Max Dilution

Western Blotting

0.5

1.0ug/ml

Immunohistology - Paraffin(1)

2ug/ml

(1)

This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice

Technical Advice

Recommended Protocol

Recommended Protocol

ELISA

ELISA

Immunohistology

Immunohistology

Histology Positive Control Tissue

Mouse heart.

Histology Positive Control Tissue

Mouse heart.

Immunofluorescence

Immunofluorescence

Western Blotting

Rabbit anti ECSIT detects a band of approximately 39kDa in human heart lysate

Western Blotting

Rabbit anti ECSIT detects a band of approximately 39kDa in human heart lysate