CLONING PCR PRODUCT

Christoph Grunau (cgrunau at rkna50.riken.go.jp) wrote:
: In article <3l53ha$do2 at taco.cc.ncsu.edu>, dyryu at unity.ncsu.edu (Doug-young
: Ryu) wrote:
: > Hi.
: >
: > I am planning to do some PCR product cloning.
: > If someone has good protocol or know any good commercial kit, please give
: > me a piece of advice.
: >
: > Thank you in advance.
: >
: > dyryu at unity.ncsu.edu
: Give the Promega pGEM-T kit a try. It's not that expensive and I had good
: results. Simply follow exactly the manual.
: Good luck - Christoph
Another vote for the pGEM-T kit. Worked for me. It's also substantially
cheaper then the Invitrogen kit, at least here in Australia it is, which
is why I chose it over Invitrogen. Big tip - buy their competent cells as
well, its not worth trying to save the money.
Loryn