Tissue Fractions

Tissue Fractions

By choosing Corning® Gentest™ tissue fractions, you will be able to conduct preclinical metabolism studies to predict the toxicity and pharmacokinetic properties of a drug compound, including metabolic stability, drug-drug interactions, reaction phenotyping, and metabolite identification. Our broad selection of liver and intestinal tissue fractions are highly characterized for important metabolizing enzymes such as CYPs, UGTs, and other enzymes. The fractions are offered as pooled or as single donors.

The pooled donors represent an average profile of catalytic activities from many individuals and are most suited for controlling the inter-individual variability in quantitative studies of metabolism. The Corning UltraPool™ HLM 150 is the first commercially available large donor pool. This product better represents the average patient population and known CYP polymorphisms, enabling consistent experimental results in multi-year programs, and offering a time savings by reducing the time required to qualify new lots of HLMs.

The single donor HLM pools offer activity levels from high to low representing the heterogeneity of expression of individual P450s found in the human population. These products provide flexibility to select from a broad range of donors at different CYP catalytic activity levels and enable to study the metabolism of specific CYPs and to phenotype and correlate CYP activities.

Many steps are taken during the manufacture of all Corning Gentest Tissue Fraction products to ensure quality and to reduce lot-to-lot variability. Corning adheres to high ethical standards and strict traceability for obtaining human materials and only sources from US-based Organ Procurement Organizations. High safety standards are also followed including serological testing prior to acceptance, followed by PCR testing for HIV I/II, Hepatitis B, Hepatitis C, and HTLV - I/II and syphilis.

Purchasing Options

It’s easy to purchase related Corning Gentest tissue fractions online. Just browse our online catalog, and add products directly to your cart. Order direct with a credit card or use a purchase order if you have an account with Corning.

It’s easy to purchase related Corning Gentest tissue fractions online. Just browse our online catalog, and add products directly to your cart. Order direct with a credit card or use a purchase order if you have an account with Corning.

Featured Products, Resources, and More

Optimizing Donor Number for Consistent Pooled HLMs

Optimizing Donor Number for Consistent Pooled HLMs

Optimizing Donor Number for Consistent Pooled Human Liver Microsomes

Based on a detailed statistical analysis of the variability in individual CYP activity levels for over 300 donors, the authors of this study conclude that the number of donors in pooled HLMs should be increased more than two-fold. A donor number of 150 appears to be a sweet spot providing low CVs for the major CYPs such that the variability in the HLM product is well below the overall assay variability. This conclusion has led to the decision to develop and commercialize Corning UltraPool HLM 150, a pooled HLM product comprised of 75 male donor livers and 75 female donor livers.

Differential Time- and NADPH-dependent Inhibition

Differential Time- and NADPH-dependent Inhibition

Differential Time- and NADPH-dependent Inhibition of CYP2C19 by Enantiomers of Fluoxetine

This application note highlights a study in which its authors compared fluoxetine, its (R)- and (S)-enantiomers, ticlopidine, and the reversible CYP2C19 inhibitor S-benzylnirvanol. In a reversible inhibition protocol (30 minute preincubation with liver microsomes without NADPH), they found (R)-, (S)-, and racemic fluoxetine to be moderate inhibitors with IC50 values of 17, 67, and 27 μM, respectively, among several other important conclusions.

This application note discusses the importance of time-dependent P450 inhibition in drug development and described a method developed by Corning Scientists. Details on assay conditions as well as best practices for running such assays are also included.

Resources and Support

FAQs: Tissue Fractions

FAQs: Tissue Fractions

Frequently Asked Questions: Corning Gentest Tissue Fractions

From storage methods and stability questions to the best method to mix microsomal suspensions, get answers to commonly asked questions about using Corning Gentest tissue fractions to support all of your metabolism-based assays.