The Dark Brotherhood is a guild of assassins with holdings all over Tamriel. A DLC pack focused on the faction's activities in the Gold Coast region and across the rest of Tamriel was released in the second quarter of 2016.

Call the weapon of the Dark Brotherhood to your hand and deliver a killing blow to an unsuspecting target. Experience from this target is reduced by 75%. This ability does not work on players or difficult targets.

After completing the first quests for the Dark Brotherhood, you can speak to Nevusa in a small enclave on the left side of the Sanctuary. She introduces you to the Litany of Blood. It holds a special assignment given by the Listener to those deemed worthy - to assassinate a number of persons throughout all faction zones. After killing each target, their ghostly image will appear on a pedestal. Executing all targets completes the Litany of Blood achievement, giving you the Executioner title and Cadaverous Assassin Polymorph.

All guilds use reputation for leveling purposes. Dark Brotherhood Reputation is earned by completing various quests and contracts for the guild. Most story quests reward 10, and some reward 20. The regular Marked for Death contracts reward 5 and the killing spree contracts reward 10. While the daily Black Sacrament contracts normally reward 10, they can reward up to 20 if all the optional objectives are completed.