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HPLC Analysis of Leucine Enantiomers on Astec® CHIROBIOTIC® T (25 °C)

Conditions

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 µm particles (12024AST)

column temp.

25 C

mobile phase

[A] water; [B] methanol; [C] formic acid; (30:70:0.02, A:B:C)

flow rate

1 ml/min

sample

300 mug /mL in 30:70 water:methanol

injection

10 µl

detector

UV, 205 nm

Description

Categories

Analytical Chromatography, Amino Acids

Featured Industry

Life Science and Biopharma

Legal Information

CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC, Astec is a registered trademark of Sigma-Aldrich Co. LLC