Zbed3 Participates in the Subcortical Maternal Complex and Regulates the Distribution of Organelles

论文题目英文:

作者:

高征①，张小欣①，宇兴江，秦丹丹，肖翼，余洋，Yun-Long Xiang，聂晓庆，卢绪坤，刘文博，#Zhao-Hong Yi，李磊*

论文出处:

年:

2017

卷:

期:

DOI: 10.1093/jmcb/mjx035

页:

联系作者:

李磊

发表期刊:

J Mol Cell Biol

ISSN:

第一作者所在部门:

收录类别:

论文链接:

https://academic.oup.com/jmcb/article/4092936

影响因子:

摘要:

We previously identified a subcortical maternal complex (SCMC) that is essential for early embryogenesis and female fertility in mice. However, the molecular mechanism by which the SCMC affects female fertility remains largely uncharacterized. Here, we report that a novel maternal protein, zinc finger BED-type containing 3 (Zbed3), participates in the SCMC. Depletion of maternal Zbed3 results in reduced fecundity of females, because of the impaired and delayed development in a proportion of mutant embryos. The loss of maternal Zbed3 results in asymmetric zygotic division and abnormal distributions of organelles in the affected oocytes and zygotes, similar to the phenotypes observed in females with disrupted core SCMC genes. Further investigation revealed that these phenotypes are associated with disrupted dynamics of microtubules and/or formation of cytoplasmic lattices (CPLs). The stability and localization of Zbed3 depend on, but are not required for, the formation of the SCMC. Thus, our data suggest Zbed3 as one of downstream proteins mediating SCMC functions and provide further insights into the roles of the SCMC and CPLs in female fertility.