Loading dye is mixed with samples for use in gel electrophoresis. It generally contains a dye to assess how "fast" your gel is running and a reagent to render your samples denser than the running buffer (so that the samples sink in the well).

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*Ficoll 400

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==Types of loading dyes==

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*Deionized water

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*Orange G dye

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Dissolve 1.5 g of Ficoll in 10 mL of deionized water. Add very small amounts of Orange G dye such that the loading dye is dark orange. Store in small aliquots at 4&deg;C (room temperature is okay too). To use, add and mix 1/5th volume of loading dye to DNA solutions prior to loading into the wells of gels.

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[[Agarose gel loading dye]]

Latest revision as of 12:53, 25 August 2006

Purpose

Loading dye is mixed with samples for use in gel electrophoresis. It generally contains a dye to assess how "fast" your gel is running and a reagent to render your samples denser than the running buffer (so that the samples sink in the well).