On-demand webinars

Versatile is smart! The power behind culturing cells on a porous membrane

There are times when your cell-based research calls for more than your standard cell culture vessels and dishes. The porous membrane and unique structure of cell culture inserts enables the versatility you need by allowing the attached surface of your cells to be exposed to growth media and different conditions simultaneously— providing easier methods for some procedures, such as cultures that require an air-liquid interface and media feeding.

In this presentation, we examine the possible uses and adaptability of cell culture inserts and review a case study using cell culture inserts to produce an artificial skin growth model at air-liquid interface.

Get the clear picture on your imaging applications workflow – from culturing and seeding your cells to image acquisition and data analysis - and how you can significantly improve your imaging results downstream by merely choosing the right culture vessel. In order to highlight the critical importance of using the right culture surface and format, we will present a case study of optimized neuron growth in chamber slides, in which we examine the correlation between neuron cell adhesion and multiple chamber slide surface modifications.

Contamination is the ever present threat to good science and your hard work. This webinar provides important points that will help you work with your biological safety cabinet more effectively to prevent contamination in your cell culture lab.

Topics Covered:

The two types of contamination and steps to address each type

Ways to safely clean different parts of your cabinet

Dealing with special cases: spills, power failures, alarms, and decontaminations

Keeping pace with innovation can be exciting (but a bit tedious at times). We want to simplify things for you, offering new, indispensable tools that can be easily inserted into your cell culture process—saving you precious time and improving consistency of your research.

During this webinar you will learn about a novel surface technology that enables consistent, repeatable growth of spheroid cultures across many different cell types, including transformed cell lines and pluripotent stem cells.

Get a Handle on Liquid Handling. Increase pipetting efficiency for reproducible results.

One of the most common tools in any cell culture laboratory is a pipette. You turn to it time and time again, and like most tools, finding the right fit for your lab is important to enable accurate and precise transferring of samples. During this webinar, we will review common requirements for cell culture applications, and key considerations to provide worry-free pipetting for confidence in your results.

You trust your work—your cells--to your CO2 incubator. So if a problem occurs, it can be catastrophic for your research, setting you back weeks or months. This webinar provides important points that will help you avoid common mistakes, reduce contamination, and extend the life of your incubator.

Topics Covered:

Proper positioning and installation of your cell culture incubator

Best procedure for cleaning and disinfection, including tips for best results with automated high heat sterilization cycles

Simple maintenance for best results, including when to calibrate, and why distilled water is important

No more separation anxiety! A research case study: Gentle, non-enzymatic detachment of sensitive cells

What if you could gently detach even the most sensitive cells without enzymes or scraping, all while maintaining the effectiveness of your process, increasing the ease and speed of detachment and preserving cell surface proteins? Please join research scientist and esteemed colleague, Marten B. Maeß from Friedrich Schiller University – Jena, Germany – as he presents his research showing the application of an innovative surface to enable transfection and non-invasive detachment of macrophages. This research case study can be applied to diverse applications and cell types, offering insights into viable alternatives to your cell detachment methods.

Topics Covered:

A research overview: Approaches to transfection and transfection of human macrophages

Optimizing macrophage transfection protocol

Detachment of cells by temperature reduction

Consistent transfection efficiency and preserved functionality of the transfected cells

Safeguarding your samples throughout the entire cryopreservation process can be tricky and even unproductive, thanks to protocols that unknowingly undermine your results. Understanding the seven keys to protecting your cryopreserved cells – from selecting the right storage temperatures and proper cooling methods to avoiding common safety hazards when using liquid nitrogen – can equip any lab with the knowledge necessary to ensure sample safety and optimal cryopreservation results. During this webinar, we will discuss each of The Seven Keys along with the benefits you can achieve as a result.

Topics Covered:

The big picture: The cryopreservation process

What you need to know about cryopreservation temperatures and cooling rates

Demystifying the tube selection process and knowing the vital features for sample safety

Take your Samples for a Spin: Fundamentals of Cell Culture Centrifugation

Separating cells is a critical step in the dynamic process of cell culture. This webinar will examine the role of centrifugation in the cell culture workflow and explore the different types of centrifuges used to separate cells. We will look at centrifuge requirements and technical considerations for cell culture applications, including selecting the appropriate speed and g-force. State-of-the-art trends in centrifugation will also be discussed.

Determining the proper sample preparation technique for long-term cryopreservation storage is of paramount importance to all downstream work stemming from the affected samples. During this webinar, we will discuss what happens during the sample preparation process, key concepts to be familiar with and key questions to consider when making decisions about long-term storage sample preparation.

Ensuring storage integrity and traceability of cells is of particular importance for cell culture applications. This webinar will provide detail related to cell preservation and storage considerations, as well as criteria for selecting appropriate storage tubes to meet your downstream cell culture needs.

Topics Covered:

Considerations for cell preservation methods

Storage tube selection criteria and why these are important to cell culture applications

Importance of maintaining sample integrity and traceability through the use of barcoded tubes and complimentary accessories

Optimizing cell culture processes is often dependant on how the cell culture fluid and media are handled. The proper selection of fluid handling equipment – pumps – can help improve efficiency and production in bio-processing research and production.

In this webinar, we give an overview of established and novel technologies used to modify cultureware surfaces for optimally growing adherent as well as non-adherent mammalian cells. We characterize the different Thermo Scientific™ Nunc™ surfaces by their adhesion properties and provide examples of how best to select the best culture surface for your application. We also present alternative methods to help improve cell harvesting.

Effective use of the Class II biological safety cabinet (BSC) protects you and your important work. Explore how BSCs use effective air filtration and controlled airflow to prevent contamination from passing into the BSC.

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