Sand goby, Oxyeleotris marmorata is a potential fish species for aquaculture in Indonesia. However, the growth performance and survival rate of the fish seed are still low. Such challenges could be solved through the optimization of stocking density of the fish. The research objective was to determine the optimal stocking density to produce high growth and survival rate, as well as the best physiological response. The ponds used in this experiment were 2 m x 1 m x 1 m in size (water volume: 1 m3). The stocking density treatments were 50, 100, and 150 individual/m3. The initial fish length average was 4.24 ± 0.58 cm, with the initial body weight average of 2.74 ± 0.45 g. During 60 days of rearing period, the fish were fed with Tubifex sp. ad libitum. The results showed that the survival rates on different stocking densities were not significantly different. The highest specific growth on length (1.50 ± 0.37%/day) and body weight total (1.95 ± 0.32%/day) and the most stable physiological response related to its hematological parameters were achieved by seed stocked at 50 individuals/m3. The best biomass total was achieved by seed stocked at 150 individuals/m3. The result of this study could be applied as basic information to culture sand goby in a controlled environment.