A 1 μl aliquot of eluate was used for amplification of a 1.2 kb fragment of the hugl gene. PCR was performed using QIAGEN HotStarTaq DNA Polymerase. No PCR inhibition was detected with 1 μl eluate per reaction. M: markers.

High-performance results in PCR — B.

A 10 μl aliquot of eluate was used for amplification of a 1.2 kb fragment of the hugl gene. PCR was performed using QIAGEN HotStarTaq DNA Polymerase. No PCR inhibition was detected with 10 μl eluate per reaction. M: markers.

Highly pure DNA.

Spectrophotometric analysis demonstrated the high purity of DNA isolated on the QIAsymphony SP.

QIAsymphony DNA Kits combine the speed and efficiency of silica-based purification of genomic DNA with the convenient handling of magnetic particles (see figure "Efficient processing of magnetic particles"). Innovative, ready-to-run reagent cartridges are prefilled with magnetic particles and all reagents required for the purification procedure, including accessory enzymes and are automatically opened by the QIAsymphony SP, which helps to minimize the risk of environmental contamination (see figure "Prefilled, sealed reagent cartridges"). Worktable setup is rapid, which saves your valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control.

Procedure

For tissues, cultured cells, and bacteria protocols, manual sample pretreatment is required. The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute. The user can choose between different elution volumes depending on the protocol. The QIAsymphony SP performs all steps of the purification procedure. Up to 96 samples are processed in a single run.