1.3 After lysis, centrifuge at 14,000 g for 5 minutes at 4°C. Transfer the supernatants to a new tube for further analysis. Aliquot and freeze in liquid nitrogen and stored at –80°C for future use. Avoid multiple freeze/thaw cycles.

2.5 After lysis, centrifuge at 14,000 g for 5 minutes at 4°C. Transfer the supernatants to a new tube for further analysis. Aliquot and freeze in liquid nitrogen and stored at –80°C for future use. Avoid multiple freeze/thaw cycles.