The French midfielder had been performing shuttle runs with the rest of the Blues squad before being forced out of action.
Bakayoko was reportedly seen grimacing in pain at Cobham after pulling up while carrying out his own individual fitness work.
According to The Sun, the 23-year-old completed a set of drills with his Chelsea team-mates then continued to do his own personalised exercises but left the pitches in agony shortly afterwards.
Bakayoko, who has been playing in pain for much of the season after knee surgery, was spotted hobbling towards the dressing room for treatment.
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