3,3'-Diaminobenzidine, tablets

Propriétés chimiques

Formule

C12H14N4

Poids moleculaire

214.27

Forme

Tablets

Storage & Sensitivity

Store at -20°C. Light Sensitive.

Solubilité

Soluble in water (partly).

Applications

3,3'-Diaminobenzidine is used to detect fingerprints in blood. 3,3'-Diaminobenzidine is used in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. Slides stained with DAB may be coverslipped in the traditional manner and stored for future reference. Many enhancement methods have been reported for DAB involving different buffer conditions, the addition of metal ions and post-treatment applications. DAB has been used in immunohistochemical staining of nucleic acids and proteins. Application of the silver-gold intensified 3,3? -diaminobenzidine chromogen to the light and electron microscopic detection of the luteinizing hormone-releasing hormone system of the rat brain. 3, 3?-diaminobenzidine (DAB)-H2O2-horseradish peroxidase (HRP) has been presented and used for the sensitive detection of carcinoembryonic antigen (CEA) in human serum.

Notes

Each tablet contains 5mg of DAB, each tablet weighs 240mg. One tablet will yield 10 ml of substrate solution, enough to effectively detect 50cm² of membrane or about 20 slides. Insoluble product. Reaction Condition: Dissolve one 5mg tablet in 10 ml of 50mM Tris, 0.1M NaCl, pH 7.6 and 0.01% hydrogen peroxide. The solution may be filtered through a 0.2µm filter immediately prior to use if necessary. The presence of nickel or cobalt increases sensitivity.

Mentions de danger et de prudence du SGH

Mentions de danger (UE):
H341-H350

Suspected of causing genetic defects. May cause cancer.

Mentions de prudence:
P201-P202-P281-P308+P313-P501c

Obtain special instructions before use. Do not handle until all safety precautions have been read and understood. Use personal protective equipment as required. IF exposed or concerned: Get medical advice/attention. Dispose of contents/ container to an approved waste disposal plant