Briefly, in each group, 100 mg of ventricle were homogenized, and then the homogenate and plasma were independently divided into two: one was kept at 37[degrees]C for 1 h which may facilitate the binding of renin (RA) to angiotensinogen producing angiotensin I (Ang I) (experiment group); the other was stored at 4[degrees]C serving as controls.

7) RAS consists primarily of an enzymatic cascade in which angiotensinogen (AGT) is converted to angiotensin I (Ang I), and subsequently to Ang II by the actions of renin and angiotensin converting enzyme (ACE), respectively.

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