アプリケーション

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション

Abreviews

特記事項

IHC-FoFr

Use at an assay dependent concentration. PubMed: 19609936

ICC/IF

Use at an assay dependent concentration.

IHC-Fr

Use at an assay dependent concentration.

IHC-P

Use at an assay dependent concentration.

WB

1/500 - 1/3000. Detects a band of approximately 181 kDa.

ELISA

1/4000 - 1/16000.

IP

1/2000.

ターゲット情報

機能

Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development.

ab7279 at 1/50 staining mouse lung tissue sections (adult, frozen 100um wholemount sections) by IHC-Fr. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4°C. An Alexa Fluor ® 488 conjugated donkey anti-rabbit antibody was used as the secondary.

ab7279 staining Robo1 in undifferentiated, immortalized human podocytes by Immunocytochemistry/ Immunofluorescence.Cells were fixed with 2% paraformaldehyde and 4% sucrose at room temperature for 10 minutes. The cells were then washed once with PBS, permeabilized with 0.3% Triton X-100 for 10 minutes and incubated with blocking solution (2% FCS, 2% BSA, 0.2% fish gelatin) for 30 minutes, before further incubation with ab7279 for 1 hour. An Alexa Fluor 488 goat anti-rabbit IgG secondary antibody was used at a dilution of 1/200. DAPI was used for nuclear counterstaining.