Figure 4.

Sensitivity of model predictions to different inputs. Effects of passage volume on (a) production of virus, and (b) production of interference
equivalents (IE). Trajectory plots for (c) virus and IE production for 20- and 150-microliter
passages, and (d) virus and IE production for 40-microliter passages. Predicted effects
of cell level on (e) virus production and (f) IE production using the experimentally
observed cell levels (solid lines), and constant cell number of 2 × 106 cell/passage (dashed lines). Effect of initial IE concentration on (g) virus production
and (h) IE production. (i) Conditions for curing of infection for virus (filled circles)
and IE (open circles); here the value of parameter q was set to zero and the simulation
was implemented for fixed-volume passages with 50 microliters. Simulation input values
for passage 1 were 1 × 109 PFU/ml, 400 IE/ml, 2 × 106 cell/passage, and 2-microliter passaged volumes from total culture well (2 ml), unless
otherwise noted.