The purity of the cultures was confirmed by Gram staining, catalaseproduction, colony morphology on blood agar and using a biochemical identification kit (API 20 Strep, bioMerieux: Marcy-IEtoile, France).

In vitro, we found that expression of a transiently transfected human ppEnk promoter-driven chloramphenicol acetyltransferase (CAT) reporter construct was induced by DAergic agonists, as much as 20-fold.

In vivo, regulation of CATexpression by DA was tested by implanting the genetically modified astrocytes into the normal striatum and the contralateral striatum which had > 95% DA depletion induced by a previous 6-hydroxy-DA lesion of the substantia nigra.

Study of Ota et al. [12] showed that women with endometriosis and adenomyosis had elevated expression of CAT in comparison to healthy women but the expression did not show the usual, fluctuating pattern characteristic for the menstrual cycle.

Macroangiopathy was associated with significantly higher frequency of C allele in SOD1 gene (0,58 in MA group vs. 0,42 in DM group without complications, p < 0,01), lower frequency of C alelle (Ala) in SOD2 gene (0,28 in MA group vs. 0,39 in DM group without vascular complications, p < 0,05) whereas no such distribution was found in CAT gene, p = 0,594.