In the BIOPHEN™ Protein C (LRT) assay, Protein C is activated with Protac®, an enzyme extracted from snake venom (Agkistrodom C Contortrix) . Activated protein C (APC) then cleaves the specific substrate SaPC-21, releasing para-nitroaniline (pNA). The yellow colour of the free pNA is measured at 405nm. There is a direct relationship between colour development and Protein C activity in the tested plasma.

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BIOPHENTM Protein C (CE-IVD)

i Chromogenic method for in vitro quantitative determination of Protein C activity on human citrated plasma, using manual or automated method. Reagents are in the liquid presentation, ready to use (LRT, Liquid Reagent Technology).