To test SWIM, Spot- and SFI-index in controlled experiments with high and low welfare conditions, by comparisons to physiological and neurobiological indicators of stress and negative affect.

To test the ability of the model to detect effects of frustration induced aggressive behaviour, as compared to CNS neurobiological and systemic physiological indicators and expression of select target genes.

WP 3. Operational field validation of the SWIM method and novel animal-based indicators