|pMQ430 is a pBAD18 derivative with the cloned dam gene, without its ribosome binding site, under control of the pBAD promoter. Expression of dam occurs in the presence of arabinose but not in its absence <cite>Calmann-Marinus-03</cite> http://users.umassmed.edu/martin.marinus/plasmid.html

|pMQ430 is a pBAD18 derivative with the cloned dam gene, without its ribosome binding site, under control of the pBAD promoter. Expression of dam occurs in the presence of arabinose but not in its absence <cite>Calmann-Marinus-03</cite> http://users.umassmed.edu/martin.marinus/plasmid.html

Transfered to MG1655 by P1 transduction from TWx23 but only grown on AB-acetate medium in case fast growth are a problem to this strain (TWx28 and TWx29 are in principle the same but have been grown in LB; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion

TWx34

MG1655(Escherichia coli)

ter::GATCcluster (ter2)

cm

Transfered to MG1655 by P1 transduction from TWx23 but only grown on AB-acetate medium in case fast growth are a problem to this strain (TWx28 and TWx29 are in principle the same but have been grown in LB; checked for not being DY330 by PCR with primers for λ-beta; checked by PCR with primers up- and downstream of the chrom. insertion

TWx35

MG1655(Escherichia coli)

ALO1739 (arok17::cat/pMS2)

cm, km

strain with less Dam molecules per cell and longer hemimethylation due to polar effects on dam expression. Used in [4]