CA15-3

This antibody has been used for evaluating the primary site of a metastatic carcinoma of unknown origin and distinguishing between benign and malignant lesions. It is believed that CA15-3 reacts primarily with the DF3-antigen, a 300 kDa mucin-like glycoprotein present on the apical border of secretory mammary epithelial cells. CA15-3 has been detected with immunohistochemistry in a wide spectrum of carcinomas, including Breast Carcinomas (ductal and lobular), Sarcomas (Synovial Sarcoma and Malignant Fibrous Histiocytomas), and Lung Carcinomas. CA15-3 can be used as a supplementary marker for epithelial differentiation. CA15-3 does not stain Melanomas or Ewing’s Sarcomas. Approximately 30% of Hepatocellular Carcinomas are positive for CA15-3.

Antibody Type

Mouse Monoclonal

Clone

DF3

Isotype

IgG1/K

Reactivity

Paraffin, Frozen

Localization

Cytoplasmic

Control

Breast, Pancreas, Salivary Gland

Presentation

CA15-3 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

Availability

Catalog No.

Antibody Type

Dilution

Volume/QTY

BSB 5099

Prediluted

Ready-To-Use

3.0 ml

BSB 5100

Prediluted

Ready-To-Use

7.0 ml

BSB 5101

Prediluted

Ready-To-Use

15.0 ml

BSB 5102

Concentrated

1:50-1:300

0.1 ml

BSB 5103

Concentrated

1:50-1:300

0.5 ml

BSB 5104

Concentrated

1:50-1:300

1.0 ml

BSB 5105

Control Slides

5

Note: For concentrated antibodies, please centrifuge prior to use to ensure recovery of all product.