The effects of a biodynamic sowing calendar on the growth (plant height, fresh herb yield, nodes number) and quality (percentage of leaf mass, essential oil content) of three basil species, Ocimum americanum L., Ocimum × hybrida and Ocimum basilicum L., represented by the cultivars ‘Rosso’ and ‘Eco Genovese’, were tested. Statistical analyses showed that the species had greater impact on the observed parameters than either the sowing date or the species and sowing date in combination. The species showed a signiﬁ cant inﬂ uence on all ﬁ ve tested parameters, while sowing date and interaction of both factors signiﬁ cantly inﬂ uenced plant height, leaf percentage and essential oil content. The best yield per plant was obtained for O. × hybrida and O. basilicum ‘Eco Genovese’. The lowest species O. × hybrida produced the highest amount of essential oil. »Nodes number« parameter most clearly separated the species, but not the cultivars. Even though O. americanum gave the tallest plants, it did not yield either the highest amount of fresh herb or essential oil. This species’ height was most consistent, considering the signiﬁ cant impact of biodynamic rhythm. Sowing date was not crucial for basil fresh yield; however if there is a need for taller plants with a higher percentage of leaf mass and more essential oil, sowing date needs to be controlled.