The PRMT4 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT4/CARM1 activity activity for screening and profiling applications. The key to the PRMT4 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated PRMT4 substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the strip plates are treated with an HRP-labeled secondary antibody followed by addition of an HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Applications:

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Storage:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.