Abstract: After the occurrence of skin lesions, the body begins the healing process in order to restore the functions of this tissue. Topical medications are used to optimize healing, however, there is increasing research for natural products for this purpose. Honey is cited as a product with healing and antimicrobial effects, but it is known that such effects depend on the floral source used by the bees. In Brazil, among the most available monoflorais honeys, are assa-peixe, cipó-uva, orange or eucalyptus. The objective of this study was to evaluate the healing and antimicrobial potential of these honeys. Firstly, the healing potential was evaluated in 84 Wistar rats submitted to a surgical wound with metallic punch 8 mm in diameter on the back of the neck. Each group of 14 animals received topical treatment, being: GC- NaCl 0.9%; GPcommercial ointment based on fibrinolysin, deoxyribonuclease and chloramphenicol on base petrolatum; Gap- assa-peixe honey; Gcu- cipó-uva honey; Geu- eucalyptus honey; Gla- orange honey. At the 2nd, 4th, 7th, 10th, 14th, 21st and 30th days after wound dressing, two animals from each group were submitted to euthanasia and macroscopic wound evaluation. In addition, a fragment of skin, subcutaneous and musculature were removed for histopathological evaluation and the area of the wound. It has been found that honeys inhibit the formation of edema and infection and render the presence of crusts transient. Orange and eucalyptus honeys reduce the area of the wound. The assa-peixe, eucalyptus or orange honeys reduce inflammation and necrosis and optimize granulation tissue growth, fibroplasia and reepithelization. In the second experiment, the in vitro antimicrobial potential of these honeys was verified in different dilutions through antibiograms against strains of Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Pseudomonas aeruginosa. It was observed that these undiluted monofloral honeys prevent growth of the main bacterial agents present in the skin, with emphasis on orange honey in the control of Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli. It was concluded that the assa-peixe, orange or eucalyptus honeys have healing and antimicrobial potential. Keywords: antibiogram, healing, honey.