RE: Processing rat brain

From:

Andrew.Shand@north-bristol.swest.nhs.uk

Spent many an unhappy hour doing that. Human cerebellum and foetal brain
being remebered with particular horror! Though I would be only to happy to
teach the technique - I suspect the researcher would not be happy to learn.
If she has any sense that is.
Andy
-----Original Message-----
From: Smith, Allen [mailto:asmith@mail.barry.edu]
Sent: 15 July 2002 15:16
To: Andrew.Shand@north-bristol.swest.nhs.uk
Cc: histonet@pathology.swmed.edu
Subject: RE: Processing rat brain
Although it is time-consuming, good sections that float off can be processed
manually, using a bent glass rod. (Heat the tip of stirring rod and jam it
against a heat-resistant table top to make a bent rod.)
Allen A. Smith, Ph.D.
Barry University
School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida 33161-6695
-----Original Message-----
From: Andrew.Shand@north-bristol.swest.nhs.uk
[mailto:Andrew.Shand@north-bristol.swest.nhs.uk]
Sent: Monday, July 15, 2002 8:46 AM
To: histonet@pathology.swmed.edu
Subject: Processing rat brain
My apologies for raising this old chestnut again but I would like to know
the details of a good schedule for processing rat brain slices. I have a
researcher with a floating section problem rather than the usual brittle
cutting.
Andy Shand