Translation of consecutive negatively charged amino acid residues destabilizes 70S ribosome and stochastically aborts translation elongation. To reveal the mechanism of this phenomenon defined as intrinsic ribosome destabilization (IRD), I performed biochemical and genetics approach. Then I revealed that specific interactions within the translating 70S ribosome complex stabilize the complex itself, and IRD disrupts them. Furthermore, I revealed the possibility that IRD could induce not only translation abortion but also expansion of the interpretation of open reading frame (ORF).

Academic Significance and Societal Importance of the Research Achievements