This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONSThis medium is for IN VITRO diagnostic use only.

STORAGEStore prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.

SIGN OF DETERIORATIONMedia should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.

PROCEDUREPrior to inoculate, the prepared media should be brought to room temperature.

Suitable anaerobes recovery is depending of collection and transportation of specimens. Specimens should be transferred into Anaerobes Pre-Reduced Transport Medium (2017) immediately after collection and brought to the laboratory quickly.

Directly, inoculate plate as soon as possible with a large amount of inoculum, streak for isolation.

Incubate at 35º C under anaerobic conditions for 2-4 days.

Examine for growth.

QUALITY CONTROLResults after 48hrs at 35ºC anaerobic atmosphere

Organisms

ATCC

Growth

Bacteroides fragilis

25285

+

Clostridium perfringens

13124

+ hemolysis

Proteus mirabilis

12453

- or partial

LIMITATIONS OF METHODThis medium is only a part of the identification. Other tests may be required.