Gelatin Methacrylate/LAP

Gelatin Methacrylate is a gelatin-based hydrogel that reacts and crosslinks in the presence of LAP and Allevi blue light technology( 1,2). The recommended preparation provided in the user instruction below yields a streamlined matrix bioink that supports 3D printed cell-laden constructs. However, preparation can be modified by users to suit their needs.

Storage and Handling

GelMA should be stored at -20 °C. LAP should be stored away from light in a dry environment.

You Will Need

Metal or UV-block needles

Petri dish or well plate for printing

Sterile filters

Optional: Treated glass (See protocol)

Note: You can purchase GelMA and LAP with needles, filters and syringes included here.

Instructions for Use

Measure 0.5% w/v LAP concentration and mix in cell media or PBS at 60°C. Mix until all LAP is dissolved.

Measure 10% w/v GelMA concentration and add to solution from step 1. Mix at 60°C until all GelMA is dissolved.

Heat solution from step 2 to 60°C and filter with a 0.45 µm syringe filter under sterile conditions.*

Cool solution to 37°C, then mix in desired cell concentration in a sterile container. *