mRNA in situ hybridization analysis of the expression
pattern of Ca2+/calmodulin-dependent protein kinase IV in
the developing thymus of a day 12.5 CD1 mouse embryo. Note the high
concentration of the mRNA transcripts (pink denotes CaMKIV
mRNA) that appear around the periphery of the developing thymus at this
age. By embryonic day 13.5 the expression of CaMKIV mRNA in the thymus
changes and becomes more diffuse throughout the organ. We have found
that the expression pattern of CaMKIV mRNA in the developing mouse
embryo is more extensive than previously suspected and is very dynamic
in regions of high cellular proliferation and differentiation. For
details, see the article by
Wang et al. in this
issue.