Chronic ultraviolet-B (UVB) irradiation of the skin results in epidermal hyperplasia, degradation of extracellular matrix, and formation of wrinkles. To investigate the anti-photoaging effect of extract of Yukmijiwhangtang (YM) in SKH-1 mice. We observed the effects of YM on skin wrinkles formation, histological changes related to epidermal thickness, collagen fibers, elastic fibers and mast cells, and immunohistochemical changes related to cytokines and enzymes in photoaging skin caused by UVB irradiation of SKH-1 mice. The oral administration YM-O and topical application of extract of YM (YM-OA) significantly decreased wrinkle formation and the epidermal thickness compared to control group (p