Peanut ELISA kit description:

Diagnostic Automation Inc. offers a Peanut ELISA Kit that is an Enzyme Immunosorbent Assay for the Qualitative Determination of Peanut in food. This Peanut ELISA Kit is a rapid and sensitive Food Allergen ELISA Kit which is designed to provide a sensitive and convenient method for screening food samples for peanut residues. The Peanut ELISA Kit represents a highly sensitive detection system and is particularly capable of the quantification of peanut residues in cookies, cereals, ice cream, and chocolate.

Peanut ELISA Test Kit Background Information:
Peanut (Arachis hypogaea) belongs to the legumes. With 25 %, the fraction of proteins in peanuts is very high. Many of these proteins are known for being allergenic, such as Arachins and Conarachins which are contained in relative high amounts. For this reason peanut represents one of the most important food allergens. For peanut allergic persons, hidden peanut allergens in food are a critical problem. Already very low amounts of peanuts can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Because of this, peanut allergic persons must strictly avoid the consumption of peanuts or peanut containing food. Cross-contamination, mostly in consequence of the production process is often noticed. The chocolate production process is a representative example. This explains why in many cases the existence of peanut residues in foods cannot be excluded. Thus, sensitive detection systems, like this Peanut ELISA Kit, for peanut residues in foodstuffs are required.

Peanut ELISA Kit Test Principle:
The Peanut ELISA Kit is a quantitative test is based on the principle of the enzyme linked immunosorbent assay. An antibody directed against peanut proteins is bound on the surface of a microtiter plate. Peanut containing samples or standards are given into the wells of the microtiter plate on the Peanut ELISA Kit.

Due to high risk of cross-contamination all applied instruments like applicator, mortar, glass vials etc. have to be cleaned thoroughly before and after each sample. Peanut proteins adhere very strongly to different surfaces. In certain cases they can resist a common dishwasher cleaning. To identify possible cross-contamination caused by previous extractions Diagnostic Automation, Inc. strongly recommends noting the sequence of the extractions. The limit of detection (LOD) of the DAI Peanut ELISA test kit is 0.1 ppm. The limit of quantification (LOQ) of the DAI Peanut test is 1 ppm. Due to the variety of sample matrices and their influence on the blank, results less than the LOQ should be treated as negative.