Three drugs effective against myxosporeans and commonly used to treat spore-forming para- sites were tested in sharpsnout sea bream (Diplodus puntazzo) infected by Enteromyxum leei. Two medicated diets were applied, one containing salinomycin and amprolium and the second containing fumagillin. Compared to untreated fish, both treatments resulted in significant reduc- tions in prevalence, intensity of all developmental myxosporean stages, and mortality. The effect was more prominent with the salinomycin and amprolium combination, where the significant reductions in intensity, prevalence, and mortality were unaccompanied by any histopathological evidence of toxic side effects or growth reduction. Sporoblasts and mature spores with distorted structures were observed in both drug treatments, but were more prevalent in the salinomycin and amprolium treatment than in the fumagillin treatment, indicating direct effectiveness on the parasite. Salinomycin with amprolium is a promising treatment for myxosporean infections in intensively cultured warmwater fish, leading to parasite elimination.