A novel setup of a biosensor that combines an EGFET (extended gate field effect transistor) and a QCM-D (quartz crystal microbalance with dissipation) has been developed. The setup utilized a commercial, affordable MOSFET, hard wired to the QCM-D without having to undergo complicated nanofabrication procedures. This sensor was capable of detecting changes in electrochemical and thickness/flexibility during bio-molecular interactions. For the proof of concept, DNA fragments of H1N1 were detected in the setup. The sensor demonstrated in situ, label-free, and multi-modal characterization of bio-molecular interactions with high sensitivity and multiple parameters through a simple instrumentation.