The GeneChip® Mouse Tiling 2.0R Array Set is designed for chromatin immunoprecipitation (ChIP) experiments. This set of seven arrays contains approximately 45 million oligonucleotide probes to interrogate the mouse genome. Each array in the set contains over 6.5 million perfect match probes to specifically interrogate genomic regions.

Sequences used in the design of the Mouse Tiling 2.0R Array Set were selected from NCBI mouse genome assembly (Build 33). Repetitive elements were removed by RepeatMasker. The Mouse Tiling 2.0R Array Set is available as a whole-genome set of seven arrays (P/N 900852).

The GeneChip® Mouse Promoter 1.0R Array is a subset of the Mouse Tiling 2.0R Tiling Array Set, and the content was chosen specifically to analyze protein/DNA interactions within promoter regions.

Probes are tiled at an average resolution of 35 base pairs, as measured from the central position of adjacent 25-mer oligos, leaving a gap of approximately 10bp between probes. Oligonucleotide probes are synthesized in situ complementary to each corresponding sequence.

Instrument and Software Requirements

Visit the Affymetrix ChIP-on-Chip Web Forum and share protocols, experiences, hints and tips on best practices for ChIP-on-chip and MeDIP-on-chip applications. Free registration is required to view or create posts.