Ordered arrays of nanostructures in metal films have been studied for practical miniaturization of SPR sensing and microfluidic integration. We report a nanostructure array that is designed to permit significantly enhanced Extraordinary Optical Transmission (EOT) with a tunable primary peak in the visible to NIR range, with the spectral shape and light transmission determined by the surface plasmon (SP) manipulation in the embedded metal film. The array structure readily interfaces with microfluidic channels, making it amenable to highly parallel throughput screening in a lab-on-chip device. The sensing platform may offer greater throughput compatibility, enhanced sensitivity of refractive index changes, improved efficacy of analyte transport, significantly increased EOT intensity for favorable signal-to-noise detection, lower cost, and rapid turnaround times; these qualities will benefit biological binding process and species detection studies and have other applications in healthcare and biomedical research. We present our progress on development of such nanostructured arrays that combines the functions of nanofluidics for effective reagent transport and nanoplasmonics for sensing platform. Our results suggest feasible development of a nano-fluidic-plasmonics-based sensing platform that can be readily integrated with microfluidics devices; hence potentially enabling in-parallel, high throughput transmission SPR lab-on-chip sensing technology.