Recombinant Mouse TNF-alpha Protein

Tumor Necrosis Factor-alpha

Tumor Necrosis Factor alpha (TNF-alpha) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, NK-cells following their stimulation by bacterial LPS. TNF-alpha activates signals through two receptors: TNF-R1, which is expressed on most cell types, and TNF-R2, which is expressed mainly on immune cells. TNF-alpha can have many functions including to stimulate of phagocytosis in macrophages, to chemoattract neutrophils, to increase insulin resistance and to induce fever. Recombinant mouse TNF-alpha is a non-glycosylated protein.

TNF-alpha is fully biologically active when compared to standard. The ED50, as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D, is less then 100 pg/ml.

Physical Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Recombinant mouse TNF-alpha is lyophilized from 0.5 x PBS, pH 7.5.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Stability

Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Endotoxin Level

Endotoxin level as measured by LAL is

Protein Content determined by

UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.