アプリケーション

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション

Abreviews

特記事項

WB

ELISA

IHC-P

ICC

追加情報

ELISA: Use at an assay dependent dilution.
ICC: 1/500.
IHC-P: Use at a concentration of 2 µg/ml.
WB: Use at an assay dependent concentration. Predicted molecular weight: 163 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

ターゲット情報

機能

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

組織特異性

Secreted in plasma.

配列類似性

Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.

発生段階

Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.

Anti-alpha 2 Macroglobulin antibody 画像

Ab58703 staining alpha 2 Macroglobulin in human liver.Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.