nested polymerase chain reaction (PCR)

nest·ed pol·y·mer·ase chain re·ac·tion (PCR)

(nest'ĕd pŏ-lim'ĕr-ās chān rē-ak'shŭn)

Use of PCR in series, so that a specified piece of DNA is amplified, and then a portion contained within the first piece is amplified further; used where extremely low amounts of DNA are present, or where there are problems with background or contaminating DNA.

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