Either you're about to have a memory panic, so Windows gave you that error as a red herring (just reboot, and it'll clear up) or the file didn't transfer cleanly (and in that case you'd download another copy and try that.)

There is a small chance your antivirus is interfering with things because its heuristic detection is acting paranoid, so you might calm it down if you can repeat the error; or it might be right on, and time to step up the antivirus scans first.

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I found this on the ipod forum on Apples website. See their site for any further assistance.

Sometimes you can resolve it by doing the following:- Download the .exe file from Apple.com/iTunes- Go to Control Panel > Add or Remove Programs- iTunes > Remove- Open C:\Program Files (default location)- Find "iTunes" (default folder name)- Shift + Delete > OK- Run a registry cleaner, such as PC Tools Desktop Maestro, to remove all old shortcuts and registry keys no longer valid on your computer.- Start > Shut Down > Restart your computer- Run the iTunes install program

This should fix the problem, however may require also to update your Windows software (update.Microsoft.com) and download the latest Visual Basic Runtime executable. One time during my clean reinstall the iTunes software mentioned an error with a vb run executable and couldn't continue the install until I found it online.

Double-check your version of Windows to make sure it is compatible - if it wasn't compatible when the player was first designed (Vista, for instance), get the updated software/firmware from Apple's website

Following is list of iPod and iTunes Error messages or Alert messages
you may come across while trying to open or install iTunes, update,
sync or restore iPod, making a purchase from iTunes Store, or perform
any other activity in your iPod/iTunes:

"Unable to Check for Purchased Music because an error occurred (-5000 error)."

"Unable to Check for Purchased Music because an error occurred (-5000 error)."

"There was an error in the iTunes Store. Please try again later."

If you come across any one of the above Error or Alert messages, then
either your iPod/iTunes has gone corrupted and your iPod may be in for
a crash or maybe you’re already facing an iPod crash. iPod might crash
or get corrupt due to any of the reasons from hardware failure to
corrupt iPod cache to damaged Files or to damaged disk structure.

You must be cautious and try to recover lost data from your iPod from
reliable and proved software which is non-destructive and can recover
all data—damaged or deleted. Any compromise can thwart your recovery
process. Try Stellar Phoenix iPod Recovery Software.