In order to study the effect of zeolite and biologic fertilizers application under different irrigation regime on yield and quantitative traits in sugar beet, a research project was conducted according split-split plot experiment based on randomized complete block design with four replicates. The main factor included irrigation regime at two levels (Normal and stress), sub factor included zeolite application at two levels (with and without application) and biological fertilizers at four levels [1- Non application of mycorrhiza, 2-Application of mycorrhiza 3- Non -inoculation of bacteria 4- Inoculation of bacteria (Pseudomonas, Azotobacter, Azospirillum)] belonged to sub-sub factor. Analysis of variance results showed that interaction effect of zeolite and mycorrhiza and non-application of bacteria under normal irrigation regime on all measured traits (Instead potassium and nitrogen content) was significant. Mean comparison of treatments indicated that NZ1M1B0 treatment (Zeolite and mycorrhiza application and non-inoculation of bacteria under normal irrigation regime) had highest amount of root yield (73340 kg.ha-1), white sugar content (11.88%) and white sugar content (16.47%) but treatments of NM1B0 (Mycorrhiza application and non-inoculation of bacteria under normal irrigation regime), DM0B0 (Non-application of mycorrhiza and bacteria under stress irrigation regime) and DZ0M0B0 (Non-application of zeolite, mycorrhiza and bacterial under stress irrigation regime) had highest amount of potassium content (4.45 meq.100g-1 sugar), amino-nitrogen (2.09 meq.100g-1 sugar) and sodium content (11.95 meq.100g-1 sugar), respectively. According to results of this research mycorrhiza inoculation and use of zeolite under water deficient conditions, caused improving sugar yield and consequently decreasing negative elements (Na, K, and N) under drought stress condition.