Datasheet Extract

Immunogen

Immunogen:

Mouse IgG whole molecule.

Add. information

OD566nm/OD280nm: 2.7

Application

Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis (1/100-1/250) as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 mg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.

Concentration

Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound).

Storage

This antibody is stable at 2-8°C as an undiluted liquid.DO NOT FREEZE!Restore with 1.0 ml of deionized water (or equivalent).Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.Dilute only prior to immediate use.Avoid repeated freezing and thawing.Shelf life: One year from despatch.