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PowerPlex® 16 HS System

The PowerPlex® 16 HS System is a multiplex STR system for use in DNA typing. This system co-amplifies the loci D18S51, D21S11, TH01, D3S1358, Penta E (labeled with fluorescein); FGA, TPOX, D8S1179, vWA and Amelogenin (labeled with TMR); CSF1PO, D16S539, D7S820, D13S317, D5S818 and Penta D (labeled with JOE). This multiplex includes all 13 CODIS STR markers, Amelogenin for gender determination and two low-stutter, highly discriminating pentanucleotide STR markers. All sixteen loci are amplified simultaneously in a single tube and analyzed in a single injection. The PowerPlex® 16 HS System is compatible with ABI...

The PowerPlex® 16 HS System is a multiplex STR system for use in DNA typing. This system co-amplifies the loci D18S51, D21S11, TH01, D3S1358, Penta E (labeled with fluorescein); FGA, TPOX, D8S1179, vWA and Amelogenin (labeled with TMR); CSF1PO, D16S539, D7S820, D13S317, D5S818 and Penta D (labeled with JOE). This multiplex includes all 13 CODIS STR markers, Amelogenin for gender determination and two low-stutter, highly discriminating pentanucleotide STR markers. All sixteen loci are amplified simultaneously in a single tube and analyzed in a single injection. The PowerPlex® 16 HS System is compatible with ABI PRISM® 310, 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems® 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.

Features - Benefits

Robustness: The PowerPlex® 16 HS System is more tolerant of PCR inhibitors than competing STR systems and the previous version of the PowerPlex® 16 System. Generate profiles with samples that previously failed to amplify. Avoid costly and time-consuming sample cleanup.

Sensitivity: Each lot is quality tested to produce full profiles from 100pg of DNA. Gain confidence in analysis of limited samples.

High Discrimination: The loci included in PowerPlex® 16 HS are more discriminating than competitive systems and are ideal for resolving partial matches or challenging familial cases.

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.

This product is available through the Promega Helix onsite stocking program in a room temperature cabinet, a –20°C Helix Freezer or a +4°C Helix Refrigerator. The program offers numerous convenient solutions to meet your lab's needs.

This product is available through the Promega Helix onsite stocking program in a +4°C Helix Refrigerator. The program offers numerous convenient solutions to meet your lab's needs. The Helix Refrigerator is 5 cubic ft.

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.

Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

Figure 1. Sensitivity of the PowerPlex® 16 HS System.

Decreasing amounts of template were amplified using the PowerPlex® 16 HS System. Each amplification was performed as directed in the PowerPlex® 16 HS System protocol. Once amplified, samples were separated on an Applied Biosystems 3130 Genetic Analyzer using 3kV, 5-second injection. In each case all expected alleles were called using a 50RFU threshold.

Decreasing amounts of template were amplified using the PowerPlex® 16 HS System. Each amplification was performed as directed in the PowerPlex® 16 HS System protocol. Once amplified, samples were separated on an Applied Biosystems 3130 Genetic Analyzer using 3kV, 5-second injection. In each case all expected alleles were called using a 50RFU threshold.

Use Restrictions

DC2101, DC2100, DW0991, DD7101, DD7251, DD1001 Not For Medical Diagnostic Use.DG1071 For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Patents - Disclaimers

DC2101, DC2100 U.S. Pat. No. 6,238,863 and other patents and patents pending.

DC2101, DC2100 Australian Pat. No. 724531, Canadian Pat. No. 2,251,793 and other patents and patents pending.

DC2101, DC2100 Allele sequences for one or more of the loci vWA, FGA, D8S1179, D21S11 and D18S51 in allelic ladder mixtures is licensed under U.S. Pat. Nos. 7,087,380 and 7,645,580, Australia Pat. No. 2003200444 and corresponding patent claims outside the US.

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.