Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

erythroleukemia

Age

35 years

Gender

male

Ethnicity

Japanese

Applications

This cell line provides a good system for investigating the proliferation and differentiation of myeloid progenitor cells.

Storage Conditions

liquid nitrogen vapor phase

Derivation

The TF-1 cell line was established by T. Kitamura, et al. in October 1987 from a heparinized bone marrow aspiration sample from a 35 year old Japanese male with severe pancytopenia. ­

Clinical Data

male

Japanese

35 years

Comments

The cells are completely dependent on interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for long term growth.

The morphological and cytochemical features, and the constitutive expression of globin genes, indicate the commitment of the cells to the erythroid lineage. Hemin and delta-aminolevulinic acid induce hemoglobin synthesis, and TPA induces dramatic differentiation of the TF-1 cells into macrophage-like cells.

The TF-1 cell line is unique because of its responsiveness to multiple cytokines.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2 ng/ml recombinant human GM-CSF; fetal bovine serum to a final concentration of 10%.

Subculturing

Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 104 viable cells/mL. Do not allow the cell concentration to reach 7 X 105 cells/mL. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.