Original Article :

Molecular analysis of rpob gene of mycobacterium tuberculosis in patients with suspected pulmonary tuberculosis in surabaya

Author :

Nurul Wiqoyah*1

Ni Made Mertaniasih*2

Manik Retno W*3

Dosen Fakultas Kedokteran

Dosen Fakultas Kedokteran

Dosen Fakultas Kedokteran

Abstract :

Tuberculosis caused by Mycobacterium tuberculosis is still a public health problem in Indonesia. Important initial step in an effort tocontrol TB and MDR-TB is TB case early finding, so early and accurate diagnosis is very important to deal with TB cases quicklyand accurately as well. Rapid molecular diagnostic methods based on molecular specific genes of Mycobacterium tuberculosisrequire the data from the genome sequence of the bacterium. RpoB gene is a gene that can be used as a bacterial diagnostic toolwhich is responsible for anti-TB drug rifampicin resistance that showed mutations in certain areas, and varied geography. So thesequences of the ropB gene of Mycobacterium tuberculosis need to be known. Research methods include the extraction andamplification of DNA regions rpoB gene of Mycobacterium tuberculosis clinical isolates from suspected of pulmonary TB patients,followed by sequencing. Ten isolates of Mycobacterium tuberculosis from sputum of patients suspected of pulmonary TB wereobtained. Amplification of a region of the rpoB gene of Mycobactrium tuberculosis resulst in a 411 bp and sequences ofMycobacterium tuberculosis rpoB gene region showed the same sequence as the nucleotide sequence of Mycobacterium tuberculosisH37Rv. These studies concluded that the region of 411 bp rpoB gene amplification product has a 100% DNA sequence homologywith Mycobacterium tuberculosis H37Rv.(FMI 2015;51:110-113)