Technical Support

Immunohistochemistry (IHC) involves the labeling of cellular proteins in tissue samples with a specific primary antibody and visualization of the target with detection reagents. Cell Signaling Technology (CST) offers over 600 antibodies validated for IHC, as well as IHC diluents, detection reagents, substrates and controls, to ensure that your IHC studies yield accurate and reproducible results. The determination of target specificity in immunohistochemical analysis requires multiple validation steps. CST scientists use a variety of methods, as appropriate, to validate each IHC-recommended antibody, ensuring that the staining you observe with each CST™ antibody is specific and believable.

Validation Steps Include

Western blot analysis is performed to demonstrate specific bands of the appropriate molecular weight(s), with minimal cross-reacting bands.

Paraffin-embedded cell pellets of known target expression levels are used to verify target specificity.