Proinsulin is modified by enzyme action to generate the mature hormone insulin in normal pancreatic beta cells. Differential staining patterns for proinsulin have been observed in certain tumors suggesting abnormal prohormone processing. This antibody may help characterize proinsulin expression in insulinomas and diabetic conditions for research applications.

Catalog #

P9008-06G

Applications

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution

Immunohistochemistry: 1:200-1:400 for 1 hr at RT

Optimal dilutions to be determined by the researcher.

Positive Control

Pancreas (cytoplasmic staining pattern)

Storage and Stability

Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.