The latest interaction between the commander in chief and first lady isn't the first to raise eyebrows over the presidential couple, and body language expert Dr. Lillian Glass warns it could be a signal of trouble in their marriage.

Click through to see the scrutinized handshake and other images from the public appearance:

"It makes them look horrible. It makes them look like their marriage is in jeopardy," Glass told AOL News. "It fuels gossip. It makes them look like they’re just in it for the show and she can’t stand him."

Glass suggests the nature of the event during which the formal greeting occurred might have played a factor, saying Trump may not fully grasp appropriate military protocol at public events.

"Many politicians know what to do, they know how to act, or they’ve been in similar situations before with foreign activities and also with the military," Glass says. "He doesn’t really know what to do. He doesn’t have that background."

From the infamous tarmac hand slap in Israel to the Slovenia native's serious facial expression during the January inauguration, the 45th president and first lady's marriage has been the subject of intense scrutiny since the former businessman took office.

Offering a bit of advice, the body language expert suggests the pair should have the guidance of a "chief of protocol" who can guide them on the "where to walk" and "what to do" elements of public appearances.

If left without help in navigating the in's and out's of public life as America's first couple, Glass suggests the Trumps may continue to suffer from the "Free Melania" headlines that often make waves when an odd public encounter occurs.