Features

Synchronization of metabolic data with food and water uptake and body mass

Multiplexed dwell time/cage as low as 30 seconds per cage and two minute cycle time for metabolic data.

Highly integrated system with real-time display

Self-calibrating with no chemical scrubbers

Description

The Promethion High Definition Multiplexed Respirometry System for rats synchronously measures energy expenditure, RQ, VO2, VCO2 and activity. High definition is fundamentally resolution with depth of field. Sable’s Promethion digital platform updates once per second for all sensors in every cage – including position, intake, wheel running, body mass and more – creating a richer data stream, secure GLP compliant raw data storage and analytical flexibility.

High resolution is dependent on constant flow and rapid exchange of air in the cage. In contrast, poorly circulating airflow caused by the add-on gantries or other push-mode limitations of conventional systems creates error prone ‘dead space.’ Sable also gives you the flexibility advantage: gas analyzers, flow generators, multiplexers, software and interfaces can be used interchangeably for rat or mouse by adding the appropriate activity detector.

This leading-edge system is designed by scientists, for scientists, to provide optimal animal enrichment without sacrificing accuracy.

Key Components*

Triple Gas Single Path Analyzer

8 Channel Flow Generator

110-120V Vacuum Pump

Promethion Interface Module

Cage Controller Data Interface

Precision Mass Monitor

Cage System with Additional Home Cage

Food Hopper

Water Bottle with Sipper Tube

Body Mass Measurement – Enrichment Accessory

Data Acquisition and Analysis Software

Data Acquisition Software for Multiplexed Promethion Systems

Optional Components*

Access Control Unit

Perforated Cage Floor

3-D IR Beam Break Array

Temperature Control Cabinet (Solid or Clear Door)

SableBus Relay

Eight Channel Flow and Calibration Multiplexer

Desktop Computer with Monitor

Environmental Monitoring Device

Instrument Rack

Heavy Duty Wire Shelf

Telemetry Bases for Temperature Activity

Telemetry Bases for Temperature, Heart Rate and Activity

Activity Wheel

*Quantity of individual components will vary, depending upon your sample size and/or number of cages.

The chart above shows the temporal resolution of a typical competitor’s multiplexed system in which several cages share a gas analysis chain (red line), compared to our new parallel, continuous system (black line). Promethion accurately tracks the energy expenditure accompanying even brief episodes of activity or inactivity, enabling quantification of rapid, subtle metabolic signals from multiple animals simultaneously with more accurate allocation of energy costs to specific behaviors than previously possible.

Promethion’s high resolution activity detection arrays capture actual activity and discard noise, giving an accurate indication of real activity (orange trace at the bottom of the graph). Body mass is shown as the black line at the top of the graph and energy expenditure (EE) is in blue. You can see that the EE drops to a low, relatively stable level when the mouse (in this case a 22 g C57BL/6J male) is inactive. The small oscillations visible in EE during inactivity are real, and are not detectable by lower-resolution systems. Then, with each bout of activity, you can see EE rising to a relatively stable level and falling back down to resting levels after the activity bout ceases. Due to Promethion’s high resolution, there is no blurring or smearing of the data.

Contact Us

For a custom configuration, contact us and our specialists will help you configure the right system for your specific research needs.

Preservation of Raw Data – Your actual study data is preserved in its original form with up to 1-second resolution, ensuring full traceability for GLP compliance or peer review. Other systems untraceably alter then discard your raw data, preventing study validation or re-analysis.

Pull-Mode Indirect Calorimetry – Secures greater accuracy and animal safety. Without a sealed cage prone to deteriorating seals, errors caused by “push-mode” leaks are eliminated. Your animal is in a low-stress environment, safeguarded in the event of a power failure.

UPCOMING COURSES

This respirometry course – held in Las Vegas, NV – teaches participants how to measure metabolic rates using indirect calorimetry. We will apply techniques for measuring real-time O2 consumption, CO2 production and water vapor loss for subjects ranging from small invertebrates to large mammals. Participants can work with single animal and high through-put systems in both open-flow[...]

This respirometry course – held in Las Vegas, NV – teaches participants how to use indirect calorimetry in metabolic screening. We will apply techniques for measuring real-time O2 consumption, CO2 production and water vapor loss for laboratory rodents. Measurement systems also include food & water intake, body mass monitoring, wheel running and voluntary activity. Data[...]