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This study was conducted to determine genetic diversity in 28 barley cultivars originated from Turkey (22 two-raw and 6 six-raw) using some physiomorphological characters and ISSR DNA molecular markers. To study some physio-morphological characters, these varieties were planted in field during 2003 - 2004 growing year. Based on this study, no any differences found among the barley varieties. To study genetic diversity in 28 barley varieties by ISSR molecular marker, 64 ISSR primers were screened...