Then, to evaluate the parasitic risk, a method was developed to estimate the viability of these eggs.

The extraction of these eggs was performed with a diphasic treatment (SDS 0.01% - Ethylacetate) coupled with a filtration on 500 mum and 100 mum sieves followed by concentration using two flotations with NaCI (d=1.19).

For the culture, 3 parameters tested showed a faster egg development at 30°C in deionized water with continuous aeration, whereas organic compounds reduced this development.

This culture was performed during respectively 13,10 and 8 days to obtain Ascaris suum, Toxocara canis and Capillaria sp larva, and 16 days for Trichuris vulpis which presented a slower development.