Confocal microscope: the microscope should have a stage‐heating system and the chamber should be pre‐heated to 37°C; set up
with excitation wavelengths of 488 and 633 nm, and emission range of 505 to 535 nm and 643 to 673 nm, for reporter and calibration
fluorophore, respectively; we use a Leica SP5 confocal laser‐scanning system with an inverted microscope