conjugate stability

In article <9507028073.AA807368724 at mail.fws.gov>,
Mail-9DE~SMTP at MAIL.FWS.GOV wrote:
>>User coler is not defined
>>Received: from net.bio.net by mail.fws.gov (SMTPLINK V2.10.03)
>X-Envelope-From: BIOSCI-REQUEST at net.bio.net>Received: (from daemon at localhost) by net.bio.net (8.6.12/8.6.6) id
>EAA29200 for diagnost-list; Wed, 2 Aug 1995 04:29:59 -0700
>Received: (from news at localhost) by net.bio.net (8.6.12/8.6.6) id
>EAA29192 for diagnost-arpanet; Wed, 2 Aug 1995 04:29:56 -0700
>To: diagnost at net.bio.net>From: burns at sasa.gov.uk (Robert Burns)
>Subject: storage of conjugates
>Date: 2 Aug 1995 04:29:45 -0700
>Sender: daemon at net.bio.net>Message-ID:
><MAPI.Id.0016.0075726e732020203632353330303030 at MAPI.to.RFC822>
>NNTP-Posting-Host: net.bio.net
>>We routinely make monoclonal AP conjugates by thiolation of the
>antibody followed by reaction with activated AP. We find that thge
>storage conditions for each conjugate is dependent on the Mab used.
>>Some conjugates store well at 4C, some don't. All of them store well
>at -20C. We have also been looking at a Pierce product called
>superfreeze which allows liquid storage of conjugates at -20C which
>should increase longevity. We find that some conjugates store better
>with it and some without. BTW in some cases conjugates stored with
>superfreeze had decreased activity compared to aliquots of the same
>batch stored at 4C without it!
>>I think with Mab conjugates it all depends on the parent antibody.
>>>Anybody else got any conjugate stories?
>>>Robert
>>>>Robert Burns
>>Monoclonal Antibody Unit
>Scottish Agricultural Science Agency
>East Craigs
>Edinburgh
>Scotland
>>burns at sasa.gov.uk>
Most conjugates can be made stable at 4C with 50% Glycerol in Picopure water.
This works for both poly and monoclonal conjugates with a variety of enzymes
as long as the protein concentration is at or above 1mg/ml. Another
reasonable stabilizer is 1% fish gelatin. Fat oxidation and free radicals can
be important when obtaining fish gelatin.
Martin Blankfard
Senior Scientist
Kirkegaard and Perry Laboratories
2 Cessna Ct.
Gaithersburg Md , 20879
Phone: (US) 1-800-638-3167 x141
FAX : 301 948 9442
E-mail: Mblank at kpl.com
08/07/95
11:41:57