The protein samples used here in the representative results section are bovine serum albumin (BSA), quantitatively succinylated BSA and mixtures thereof yielding BSA samples with defined succinylation occupancy at 0%, 1%, 10%, 50%, and 100%, respectively. This protocol has also been performed using protein lysates from E. coli and mouse liver. The protocol can also be applied to other cell or tissue lysates.