Anti-TSG101 抗体 [4A10] (ab83)

製品の概要

特異性This antibody recognizes the TSG-101 protein, the product of a recently identified tumor susceptibility gene the inactivation of which in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice.

ターゲット情報

機能Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.

ドメインThe UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.The coiled coil domain may interact with stathmin.The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.

翻訳後修飾Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.

細胞内局在Cytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.

Astrocytes were fixed with paraformaldehyde and permeabilized with 0.2% Triton X-100 for 5 min at room temperature. Cells were incubated with primary antibody and a fluorescent-conjugated anti-mouse IgG was used as the secondary antibody.

Panc-1 and Serum Exosomes were characterized by the exosome-specific expression of TSG101 by FACS analysis.

Exosomes were attached to 4-μm aldehyde/sulfate latex beads by mixing ∼30 μg of exosomes in a 100-μl volume of beads for 2 hours at room temperature. This suspension was diluted to 1 ml with PBS, and the reaction was stopped with 100 mm glycine and 2% BSA in PBS. Exosome-bound beads were washed in PBS/1% BSA, blocked with 10% BSA, and stained for FACS with anti-TSG101 (1:400, ab83). An Alexa Fluor® 488 conjugated anti-mouse IgG was used as the secondary antibody.

There could be many reasons for this extra band. It could be an Isoform; TSG101 have 2 isoforms one is 31kDa nd other is 44 kDa. Two bands could be observed however it will depend on the lysates being used. http://w...

The datasheet was correct and the customer should have received 100ul, I apologise for this problem and have arranged a free of charge vial to be sent to you.
Many thanks for your help with this matter,

Thank you for your email. I contacted the originator of this antibody who was able to provide me with the following information. "Concerning IHC staining, you may want to refer to the following publication for a detailed protocol: Zhu et at, Oncogene 2...

Thank you for the details that you have provided and I'm sorry to hear that your customer is experiencing difficulty with this antibody. You mentioned that a previously purchased batch worked well; do you have that batch number? Were the protocols and ...