Project Description/Abstract

Synthetic biology is an exciting new field that aims to create

novel biological functions by artificially combining natural molecular
parts. Rational design allows us to engineer the behaviors of
protein-based drugs, in ways that we cannot accomplish with small
molecule-based drugs. DNA assembly will be used to build a combinatorial
library of over 200 synthetic transcription factors (Pc-Tfs) that will
potentially activate therapeutic genes in cancer and stem cells. As time
allows, a select group of Pc-TF protein will be purified and analyzed
biochemically.

Notes

Brady Laughlin's Spring 2012 project in the Haynes Lab at Arizona State University