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This week, I talk with Madison (Madi) Shipley. She is a graduate student (MS) in the School of Aquatic and Fishery Sciences, at the University of Washington. We talk about her thesis work of developing a management strategy evaluation for Tanner crab in the Eastern Bering Sea, her job with the Natural Resources Consultants Incorporated, … Continue reading De […]

This week we talk about transcriptome assembly (with our new RNAseq data from NWGC!!) and next steps. Also, we decide that Qiagen RNeasy Kit works pretty well for extracting RNA, and will be used to create the remaining libraries for the project.

This week Pam tells us about her recent experience on a survey on the Bering Sea, and we discuss our new plan. The original RNA extraction method using RNAzol RT resulted in low RNA yields, as well as contaminated samples, likely due to excess salts. We have submitted a pooled sample to the NWGC for … Continue reading DecaPod S1E11: Crab Meeting #5

This week we discuss our plan of having three pools sequenced and next steps going forward. Three pools for the current three libraries are as follows: uninfected from Day 9 (before temperature treatment began) infected from Day 9 (before temperature treatment began) “MasterPool” : 10 samples. One sample per each of the following treatments: uninfected, … Co […]

This week I speak with Genevieve Johnson, a MS student at University of Alaska, Fairbanks. We talk about her thesis work on population genetics of Alaskan Tanner Crabs, her experience taking crab samples on a vessel, and her thoughts on her future.