HitHunter® cAMP and cAMP Hunter™ Cell Line: Your Solution for Use with High Serum Samples, Difficult Gi Targets and Identification of Allosteric Modulators

The cAMP detection technologies are widely used in high throughput screening (HTS) for measuring a functional response of a GPCR consequent to ligand-binding. However, a robust and sensitive cAMP detection platform that allows one to differentiate a full agonist from a partial agonist or an antagonist response from an inverse agonist response while also being able to identify allosteric compounds is preferred. DiscoveRx’s HitHunter® cAMP assay, a proven and validated platform, provides an enzyme-fragment complementation format that detects cAMP in cell lines, primary cells, neuronal cell lines, membrane preparations and in patient samples. Large assay window, high sensitivity, ability to work with very low cell number and compatibility with any standard luminometer are hallmarks of HitHunter® cAMP assays. These features offer a tremendous advantage for Gi-coupled GPCRs over other available kits in the market. In this study, we demonstrate the assay compatibility with high serum levels, antibody screening and finally superiority of the platform in screening several difficult to optimize Gi cell lines. The data shows the versatility of the HitHunter® cAMP assay that provides a researcher or a high throughput screener a simple one or two step chemiluminescent assay for cAMP detection.