Summary

Secondary Antibodies

Negative Isotype Controls

Useful Reagents

Positive Controls

Histology Controls

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References

Reviews

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Rabbit anti Mouse CXCL1 antibody recognizes mouse CXCL1 also known as GRO1 and KC, a 7.8kDa chemokine involved in neutrophil chemotaxis and activation, and expressed in a range of cells including monocytes, macrophages and vascular endothelial cells.

Intended Use

Target Species

Mouse

Product Form

Purified IgG conjugated to Biotin - lyophilised

Purified IgG - lyophilised

Reconstitution

Reconstitute with 1.0ml sterile PBS containing 0.1% bovine serum albumin Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.

Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

Preparation

Antiserum Preparation

Antisera to mouse CXCL1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name

Yes

No

Min Dilution

Max Dilution

ELISA

0.15ug/ml

0.30ug/ml

Western Blotting

0.1ug/ml

0.2ug/ml

Application Name

Yes

No

Min Dilution

Max Dilution

ELISA

0.5

2.0ug/ml

Western Blotting

0.1

0.2ug/ml

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Technical Advice

Technical Advice

Recommended Protocol

Recommended Protocol

ELISA

AAM40B may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with AAM40 as the capture antibody and PMP66 as the standard.

ELISA

AAM40 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with AAM40B as the detection antibody and PMP66 as the standard.

Immunohistology

Immunohistology

Histology Positive Control Tissue

Histology Positive Control Tissue

Immunofluorescence

Immunofluorescence

Western Blotting

AAM40B may be used under either reducing or non-reducing western blotting conditions with PMP66 as the positive control.

Western Blotting

AAM40 may be used under either reducing or non-reducing western blotting conditions with PMP66 as the positive control.