With So Many Different Compartments: How To Know Which is Which? ? ? ? ? ? ? ? ?

Antibodies Can Be Used To Detect Specific Proteins Different types of tags can be coupled to secondary antibodies: • Gold beads, for Immuno-electron microscopy • Fluorescent molecules, for Immunofluorescence • Enzymes for immunoblotting or ELISA

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Differential Centrifugation: One Way of Separating Subcellular Organelles Organelles are separated Based on their relative Size/Density

Rate-zonal Centrifugation Rate-zonal Centrifugation separates Particles based on their relative size (Mr) M(r) = Molecular radius, a combination of Both size and density-e.g. both a golf ball and ping-pong ball are about the same size, but the M(r) of the golf ball is much higher because it is much denser Differential centrifugation is a form of Rate-zonal centrifugation Sucrose, or other high density liquid is used to increase the amount of time it takes for particles to sediment to the bottom of the tube, allowing for greater resolution of particles of different sizes

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