The Schleicher group is currently working with 13 Ste20-kinases (excluding the Paks A to D) that have a possible regulatory domain C-terminal of the kinase domain, usually a hallmark for GCK-subfamily kinases.
To elucidate the function of these kinases we are analyzing the phenotypes of knockouts and GFP-fusion proteins.
So far, we were NOT successful in knocking out Krs2 (DDB0216375), a protein that contains four repeats of a calpain-domain-III, and PakG (DDB0229411). For the other 11 Ste20-like kinases knockout mutants have been isolated.

The nomenclature is based on phylogenetic relationships. Dst stands for Dictyostelium Ste20-like kinase and should make association to the well characterized MSTs in higher organisms. PakE to PakG is the nomenclature suggested by Goldberg et al. based on the presence of functional PBD/CRIB domains N-terminal of the kinase domain. DFray names the D. discoideum homologs of the drosophila kinase fray.