Gobind Khorana is, was at that time the, one of the world's best organic chemists working in the field of nucleic acids, highly experienced and superbly equipped to do this. He synthesized the 64 triplets chemically and he also synthesized repeating polymers with known doublets, repeating doublets or triplets as well, and towards the end he used them all to determine nucleotide sequences also. So between the two labs, he used the ribosome binding assay that we had established and between the two labs we determined all of the nucleotide sequences of RNA codons.

Har Gobind Khorana, Marshall Nirenberg, and Robert Holley shared the 1968 Nobel Prize for Physiology and Medicine. Nirenberg and Khorana cracked the genetic code. Holley sequenced and deduced the structure of the first tRNA molecule.

Marshall Nirenberg, Har Gobind Khorana, and Robert Holley shared the 1968 Nobel Prize for Physiology and Medicine. Nirenberg and Khorana cracked the genetic code. Holley sequenced and deduced the structure of the first tRNA molecule.

After the easy codons, exact triplets had to be made in order to finish deciphering the rest. Marshall Nirenberg and a group of scientists including Maxine Singer, Marianne Grunberg-Manago, Phil Leder were involved in this process. Har Gobind Khorana al

Marshall Nirenberg and Heinrich Matthaei used poly-U mRNA in a cell-free system to make a polyphenylalanine protein chain. This showed that UUU must be the code that specifies the amino acid phenylalanine.