Title

Author

Faculty Advisor

Rulfs, Jill

Sponsor

Massachusetts Institute of Technology Biomaterials

Abstract

Two monosaccharide labeling agents, 1-phenyl-3-methyl-5-pyrazolone (PMP), and 4-(3-Methyl-5-oxo-2-pyrazolin-1-yl) Benzoic Acid (PMPA), were investigated to determine monosaccharide content in palm oil mill effluent waste (POME). Methods published in the literature were optimized to increase efficiency of monosaccharide detection to suit industry needs. The results from PMP derivatization, using pH 8.0 PBS for dilution and microwave irradiation suggest this is the most efficient derivatization method tested in this project. Monosaccharides detected in POME samples were mannose, glucose and xylose.

Additional files

Copyright Statement

All authors have granted to WPI a nonexclusive royalty-free license to distribute copies of the work, subject to other agreements. Copyright is held by the author or authors, with all rights reserved, unless otherwise noted.