Tim-Tipin complex might play a role in coupling DNA unwinding and DNA synthesis by directly affecting the catalytic activities of replication fork proteins.

We now show that cellular DNA replication fork pausing and protection factors Timeless (show TIMELESS Antibodies) (Tim) and Tipin (Timeless-interacting protein) accumulate at OriP during S phase of the cell cycle.

The results suggest that Timeless (show TIMELESS Antibodies)-Tipin functions as a replication fork stabilizer that couples DNA replication with sister chromatid cohesion established at replication forks.

Tipin is a checkpoint mediator that cooperates with Tim and may regulate the nuclear relocation of Claspin (show CLSPN Antibodies) in response to replication checkpoint

observation explains the similar checkpoint phenotypes observed in both Tipin- and Timeless (show TIMELESS Antibodies)-depleted cells

TIM and Tipin are functional orthologs of their replisome-associated yeast counterparts capable of coordinating replication with genotoxic stress responses, and distinguishes mammalian TIM from the circadian-specific paralogs.

TIM and Tipin are functional orthologs of their replisome-associated yeast counterparts capable of coordinating replication with genotoxic stress responses, and distinguishes mammalian TIM from the circadian-specific paralogs.

TIPIN Antigen Profile

Protein Summary

Required for normal progression of S-phase. Important for cell survival after DNA damage or replication stress. May be required in the replication checkpoint induced by hydroxyurea or ultraviolet light (By similarity).