CD172a antibody | CC149

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Published customer image:Mouse anti Bovine CD172a antibody, clone CC149 (MCA2041GA) used for the evaluation of CD172a expression on ovine bone marrow aspirate cells by flow cytometry.Image caption:BM of Csf1r-EGFP sheep contains a heterogeneous EGFP+ population of cells. BM from Csf1r-EGFP sheep was isolated from ribs and analyzed via flow cytometry. (A) Gating strategy. Dead cells were excluded with propidium iodide staining and granulocytes excluded via FSC/SSC. EGFPlo and EGFPhi were selected once quadrants were set with negative control BM. (B) Analysis of CD14, CD16, and CD172a expression in GFPlo and GFPhi cells. Quadrants were set using isotype controls for each population. (C) Leishman-stained EGFP+ cells (combined EGFPlo and EGFPhi). Scale bar, 10 μm. Dot plots and Leishman staining of EGFP+ cells representative of five and two sheep, respectively. Leishman staining was representative of 15 images per animal.

From: Waters WR, Palmer MV, Nonnecke BJ, Thacker TC, Estes DM, et al. (2009)Signal Regulatory Protein α (SIRPα)+ Cells in the Adaptive Response to ESAT-6/CFP-10 Protein of Tuberculous Mycobacteria.PLoS ONE 4(7): e6414.This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Published customer image:Mouse anti Bovine CD172a antibody, clone CC149 (MCA2041GA) used for the identification of CD172a expressing cells in bovine blood by flow cytometry.Image caption:Adaptation of the reverse-transmigration in vitro model with bovine cells to recapitulate monocyte-derived cells mobilization from blood to the draining lymph node under inflammatory condition. A-D Schematic representation of the in vitro differentiation of blood monocytes into macrophages and Mo-DCs using the reverse-transmigration model. E-I Two-color flow cytometry assay of monocyte-derived cells collected from monocyte/BAEC cultures grown on collagen-coated transwells according to the reverse-transmigration protocol. Non-adherent and low-adherent cells within the top and bottom sections of the transwell were processed for cytometry analysis. Left contour plots show the conditions in the absence of any exogenous stimulus whereas right contour plots show conditions with zymosan. Cells were labeled with anti-SIRP1-α to discriminate monocyte-derived cells from endothelial cells. The expression of MHCII within the SIRP1-α+ population was also determined to discriminate within monocyte-derived cells potential macrophages (MHCII+) and potential Mo-DC (MHCIIhi) (E). The percentages of SIRP1-α+MHCII+, SIRP1-α+MHCIIhi and BAECs in each representative dot plot are indicated (E). F Comparison showing the relative number of SIRP1-α+MHCII+ cells in the bottom of the transwell in conditions with or without zymosan stimulation. G Comparison showing the relative number of SIRP1-α+MHCII+ cells in the top section of the transwell in conditions with or without zymosan stimulation. H Comparison showing the relative number of SIRP1-α+MHCIIhi cells in the top section of the transwell in conditions with or without zymosan stimulation. i Comparison showing the relative expression level of MHCII in SIRP1-α+MHCII+ cells in the top section of the transwell in conditions with or without zymosan stimulation. F-I The value of the control condition was arbitrarily set to 1. Data are presented as the mean ± SD based on four independent experiments each including at least three transwells per condition. Statistically significant differences between the two groups were determined by the Student's t test, *P <0.05 and **P <0.005.

From: Vachiery N, Puech C, Cavelier P, Rodrigues V, Aprelon R, Lefrançois T, Martinez D, Epardaud M. An in vitro model to assess the immunosuppressive effect of tick saliva on the mobilization of inflammatory monocyte-derived cells.Vet Res. 2015 Sep 28;46(1):117.
Published customer image:Mouse anti Bovine CD172a antibody, clone CC149 (MCA2041GA) used for the identification of CD172a expressing cells in bovine blood by flowcytometry.Image caption:In vitro characterization of the dual immunosuppressive effect of tick saliva on inflammatory-induced mobilization of monocyte-derived mononuclear phagocytes. A-E Two-color flow cytometry assay of monocyte-derived cells collected from monocyte/BAEC cultures grown on collagen-coated transwells for 48 h according to the reverse-transmigration protocol. Left contour plots show conditions with zymosan alone whereas right contour plots show conditions with zymosan and tick saliva. B Comparison showing the relative number of SIRP1-α+MHCII+ cells in the bottom of the transwell in conditions with zymosan alone or with zymosan and saliva. C Comparison showing the relative number of SIRP1-α+MHCII+ cells in the top section of the transwell in conditions with zymosan alone or with zymosan and saliva. D Comparison showing the relative number of SIRP1-α+MHCIIhi cells in the top section of the transwell in conditions with zymosan alone or with zymosan and saliva. E Comparison showing the relative expression level of MHCII in SIRP1-α+MHCII+ cells in the top section of the transwell in conditions with zymosan alone or with zymosan and saliva. B-E The value for the control condition (zymosan alone) was arbitrarily set to 1. Data are presented as the mean± SD based on four independent experiments each including at least three transwells per condition. Statistically significant differences between the two groups were determined by the Student's t test, *P < 0.05 and **P <0.005. (F&G) Representative histograms of MHCII and CD86 staining on SIRPα+ monocyte-derived cells incubated for 48 h with zymosan and saliva at amounts varying from 0 to 55 μg/mL. One representative experiment out of two independent experiments is shown. G Schematic representation of the effect of tick saliva on the mobilization of monocyte-derived APCs in vivo. (1) Monocytes from the blood are recruited to the area of the tick bite where a proportion of the cells differentiate into potential macrophages. (2) Potential Mo-DC precursors then migrate from this area into the draining lymphatic vessels.

Mouse anti Bovine CD172a antibody, clone CC149 recognizes bovine CD172a, also known as MyD-1 antigen and SIRPA. CD172a is a ~55 kDa single pass type 1 membrane protein belonging to the family of signal regulatory proteins (SIRP). CD172a has been identified as the receptor for CD47.

Bovine CD172a is strongly expressed by splenic macrophages, monocytes and a subset of afferent lymph veiled cells (ALVC) and by dendritic cells in the skin.

Product Details

Target Species

Bovine

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) -Cy5 - lyophilized

Reconstitution

Reconstitute with 1.0ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

Storage Information

Storage

Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage

Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Applications of CD172a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name

Verified

Min Dilution

Max Dilution

Flow Cytometry

1/100

1/200

Flow Cytometry

Neat

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry

Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Flow Cytometry

Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Published customer image:Mouse anti Bovine CD172a antibody, clone CC149 (MCA2041GA) used for the evaluation of CD172a expression on ovine bone marrow aspirate cells by flow cytometry.Image caption:BM of Csf1r-EGFP sheep contains a heterogeneous EGFP+ population of cells. BM from Csf1r-EGFP sheep was isolated from ribs and analyzed via flow cytometry. (A) Gating strategy. Dead cells were excluded with propidium iodide staining and granulocytes excluded via FSC/SSC. EGFPlo and EGFPhi were selected once quadrants were set with negative control BM. (B) Analysis of CD14, CD16, and CD172a expression in GFPlo and GFPhi cells. Quadrants were set using isotype controls for each population. (C) Leishman-stained EGFP+ cells (combined EGFPlo and EGFPhi). Scale bar, 10 μm. Dot plots and Leishman staining of EGFP+ cells representative of five and two sheep, respectively. Leishman staining was representative of 15 images per animal.

From: Waters WR, Palmer MV, Nonnecke BJ, Thacker TC, Estes DM, et al. (2009)Signal Regulatory Protein α (SIRPα)+ Cells in the Adaptive Response to ESAT-6/CFP-10 Protein of Tuberculous Mycobacteria.PLoS ONE 4(7): e6414.This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Published customer image:Mouse anti Bovine CD172a antibody, clone CC149 (MCA2041GA) used for the identification of CD172a expressing cells in bovine blood by flow cytometry.Image caption:Adaptation of the reverse-transmigration in vitro model with bovine cells to recapitulate monocyte-derived cells mobilization from blood to the draining lymph node under inflammatory condition. A-D Schematic representation of the in vitro differentiation of blood monocytes into macrophages and Mo-DCs using the reverse-transmigration model. E-I Two-color flow cytometry assay of monocyte-derived cells collected from monocyte/BAEC cultures grown on collagen-coated transwells according to the reverse-transmigration protocol. Non-adherent and low-adherent cells within the top and bottom sections of the transwell were processed for cytometry analysis. Left contour plots show the conditions in the absence of any exogenous stimulus whereas right contour plots show conditions with zymosan. Cells were labeled with anti-SIRP1-α to discriminate monocyte-derived cells from endothelial cells. The expression of MHCII within the SIRP1-α+ population was also determined to discriminate within monocyte-derived cells potential macrophages (MHCII+) and potential Mo-DC (MHCIIhi) (E). The percentages of SIRP1-α+MHCII+, SIRP1-α+MHCIIhi and BAECs in each representative dot plot are indicated (E). F Comparison showing the relative number of SIRP1-α+MHCII+ cells in the bottom of the transwell in conditions with or without zymosan stimulation. G Comparison showing the relative number of SIRP1-α+MHCII+ cells in the top section of the transwell in conditions with or without zymosan stimulation. H Comparison showing the relative number of SIRP1-α+MHCIIhi cells in the top section of the transwell in conditions with or without zymosan stimulation. i Comparison showing the relative expression level of MHCII in SIRP1-α+MHCII+ cells in the top section of the transwell in conditions with or without zymosan stimulation. F-I The value of the control condition was arbitrarily set to 1. Data are presented as the mean ± SD based on four independent experiments each including at least three transwells per condition. Statistically significant differences between the two groups were determined by the Student's t test, *P <0.05 and **P <0.005.

From: Vachiery N, Puech C, Cavelier P, Rodrigues V, Aprelon R, Lefrançois T, Martinez D, Epardaud M. An in vitro model to assess the immunosuppressive effect of tick saliva on the mobilization of inflammatory monocyte-derived cells.Vet Res. 2015 Sep 28;46(1):117.
Published customer image:Mouse anti Bovine CD172a antibody, clone CC149 (MCA2041GA) used for the identification of CD172a expressing cells in bovine blood by flowcytometry.Image caption:In vitro characterization of the dual immunosuppressive effect of tick saliva on inflammatory-induced mobilization of monocyte-derived mononuclear phagocytes. A-E Two-color flow cytometry assay of monocyte-derived cells collected from monocyte/BAEC cultures grown on collagen-coated transwells for 48 h according to the reverse-transmigration protocol. Left contour plots show conditions with zymosan alone whereas right contour plots show conditions with zymosan and tick saliva. B Comparison showing the relative number of SIRP1-α+MHCII+ cells in the bottom of the transwell in conditions with zymosan alone or with zymosan and saliva. C Comparison showing the relative number of SIRP1-α+MHCII+ cells in the top section of the transwell in conditions with zymosan alone or with zymosan and saliva. D Comparison showing the relative number of SIRP1-α+MHCIIhi cells in the top section of the transwell in conditions with zymosan alone or with zymosan and saliva. E Comparison showing the relative expression level of MHCII in SIRP1-α+MHCII+ cells in the top section of the transwell in conditions with zymosan alone or with zymosan and saliva. B-E The value for the control condition (zymosan alone) was arbitrarily set to 1. Data are presented as the mean± SD based on four independent experiments each including at least three transwells per condition. Statistically significant differences between the two groups were determined by the Student's t test, *P < 0.05 and **P <0.005. (F&G) Representative histograms of MHCII and CD86 staining on SIRPα+ monocyte-derived cells incubated for 48 h with zymosan and saliva at amounts varying from 0 to 55 μg/mL. One representative experiment out of two independent experiments is shown. G Schematic representation of the effect of tick saliva on the mobilization of monocyte-derived APCs in vivo. (1) Monocytes from the blood are recruited to the area of the tick bite where a proportion of the cells differentiate into potential macrophages. (2) Potential Mo-DC precursors then migrate from this area into the draining lymphatic vessels.