ET can exclusively confirm that Oprah, 60, will be playing Richard Pryor's grandmother, Marie Carter, in Lee Daniels' highly anticipated biopic on the legendary comedian.

Pryor, who died from a heart attack in 2005, had a notoriously complicated relationship with his grandmother. Pryor grew up in a house of prostitution run by Marie, when his mother Gertrude walked out on the family when he was 10 years old.