A: Plasma membrane and nuclear periphery localization of the
SH3BP4 fusion proteins. B: Nuclei from ARPE-19 transfected with
GFP-SH3BP4 for 24 h were purified using NE-PER reagent. Confocal and
epifluorescence microscopy show GFP-SH3BP4 at the nuclear periphery. The
corresponding phase microscopy image for the epifluorescent nuclei is
also shown. C: Confocal photomicrographs of APRE-19 transfected
with GFP-SH3BP4 for 24 h and stained with the plasma membrane marker
CM-DiI. GFP-SH3BP4 at the cell periphery co-localizes with the plasma
membrane marker CM-DiI seen in yellow in the merged image. Bars
represent 20 μm.