In order to compare yields between our kits and the leading competitor,
side-by-side extractions were performed on aliquots from single cultures
of various strains of E.coli containing different plasmids. The results
are shown in Table 1. Clearly, the Perfectprep kits significantly outperformed
the leading competitor's kit in terms of yield every time.

Table 1: Perfectprep Midi vs. Brand X

Plasmid/Host

Culture Volume

PP Midi Yield

Brand X Yield

Average PP percentage of Leading Brand

Overall average PP percentage of Brand X

pEGFP-N1/XL-1 Blue

25 ml

201 g

133 g

147%

344%

185 g

128 g

pGEMgapdh/HB101
TD>

25 ml

137 g

27 g

592%

155 g

22 g

pCH110/JM109

75 ml

80 g

34 g

292%

148 g

44 g

Next we tested product purity by restriction digests of 250 ng of the
pCH110 DNA. As is shown in figure 1, all samples cut to completion with
both kits.
Figure 1: Perfectprep Midi DNA and Brand
X DNA Restriction Digest
Restriction digests using EcoR1 from NEB were performed on 250 ng of the
pCH110 DNA samples tabulated above. Lane order is as follows:

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