目标详细情况

目标详细情况

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别名

V5 Tag

物质类

Tag

背景

The V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.

使用细节

使用细节

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应用备注

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.1 µg/mLWestern blot: 0.2-1 µg/mL Immunoprecipitation: 1-5 µg/mL FACS: 1-3 µg for 1 x 10^6 cellsICC/IF: 1-3 µg/mL Fusion Partner Fusion of SP2/0-Ag14 myeloma and B-lymphocytes