Application Information: RAD23B antibody [5H1-A10-A7]

Recommended Starting Dilutions:
For WB: Use at a dilution of 1:1000.
For ICC/IF: Use at a dilution of 1:100.
For IHC-P: Use at an assay depeendent dilution.
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.

Form Supplied

Liquid

Concentration

1.5
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Affinity purified

Specifications: RAD23B antibody [5H1-A10-A7]

Full Name

RAD23 homolog B (S. cerevisiae)

Background

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2011]