性能

Primary antibody说明The antiserum was tested using the free floating PAP technique on rat dopaminergic areas. The anti-conjugated Dopamine antibodies gave good staining between 1/2000-1/5000 dilution in these areas.

靶标

相关性Dopamine (C6H3OH)2-CH2CH2NH2)is a catecholamine neurotransmitter in the brain. Its chemical name is 4-(2-aminoethyl)benzene-1,2-diol. Dopamine is a hormone released by the hypothalamus. Its main function is to inhibit the release of prolactin from the anterior lobe of the pituitary. It can be used as a sympathomimetic drug, producing effects such as increased heart rate and blood pressure.

细胞定位Secreted

别名

3 4 dihydroxyphenethylamine antibody

3 hydroxytyramine antibody

DA antibody

Dopamine antibody

Oxytyramine antibody

see all

Anti-Dopamine antibody 图像

ab6427 at 1/5000 staining human stem cells (differentiated to neurons) by ICC. The cells were paraformaldehyde fixed and blocked with BSA before incubation with the antibody for 2 hours. A PE conjugated goatanti-rabbit antibody was used as the secondary.

ab6427 staining dopamine in PC12 cells treated with ghrelin (rat) (ab120231), by ICC/IF. Decrease in dopamine expression correlates with increased concentration of ghrelin, as described in literature.The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120231 (ghrelin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6427 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

ab6427 is not guaranteed for use in immunohistochemistry. However, ab1001 (http://www.abcam.com/ab1001) is guaranteed to work in immunohistochemistry and gave good results when fixed with formalin/PFA in paraffi...

We have additional information about capturing the DA as follows:
After discussion with our research team, we think that the better way is to bind DA on your tissue via glutaraldehyde (0.1 to 1%).
- Double bounds are reduced by adding of sodium bor...

This antibody is known to work with 3-5% glutaraldehyde perfusion. Protocol is as follows -
Perfusion : perfuse intracardially through the aorta using a pump with the following solutions :
solution A (30ml) : 200-300ml/min
solution B (500ml) : 200-...