DC-UbP/UBTD2 is a ubiquitin (Ub) domain-containing protein first identified from dendritic cells, and is implicated in ubiquitination pathway. The solution structure and backbone dynamics of the C-terminal Ub-like (UbL) domain were elucidated in our previous work. To further understand the biological function of DC-UbP, we then solved the solution structure of the N-terminal domain of DC-UbP (DC-UbP_N) and studied its Ub binding properties by NMR techniques. The results show that DC-UbP_N holds a novel structural fold and acts as a Ub-binding domain (UBD) but with low affinity. This implies that the DC-UbP protein, composing of a combination of both UbL and UBD domains, might play an important role in regulating protein ubiquitination and delivery of ubiquitinated substrates in eukaryotic cells.

DC-UbP/UBTD2 is a ubiquitin (Ub) domain-containing protein first identified from dendritic cells, and is implicated in ubiquitination pathway. The solution structure and backbone dynamics of the C-terminal Ub-like (UbL) domain were elucidated in our previous work. To further understand the biological function of DC-UbP, we then solved the solution structure of the N-terminal domain of DC-UbP (DC-UbP_N) and studied its Ub binding properties by NMR techniques. The results show that DC-UbP_N holds a novel structural fold and acts as a Ub-binding domain (UBD) but with low affinity. This implies that the DC-UbP protein, composing of a combination of both UbL and UBD domains, might play an important role in regulating protein ubiquitination and delivery of ubiquitinated substrates in eukaryotic cells.

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dc.language

eng

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dc.publisher

Wiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.proteinscience.org

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dc.relation.ispartof

Protein Science

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dc.rights

The definitive version is available at www3.interscience.wiley.com

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dc.subject.mesh

Dendritic Cells - chemistry

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dc.subject.mesh

Nuclear Magnetic Resonance, Biomolecular - methods

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dc.subject.mesh

Protein Structure, Tertiary - genetics

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dc.subject.mesh

Ubiquitin - chemistry - metabolism

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dc.subject.mesh

Ubiquitins - chemistry - genetics - metabolism

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dc.title

Solution structure of the N-terminal domain of DC-UbP/UBTD2 and its interaction with ubiquitin