Why does my gene of interest not show up in the Gene Knockout Kit portal?

We do not currently offer the GKO Kit for all human genes. Some genes are excluded from the offer because we cannot guarantee knockout with them. This is because the guides available in that gene do not satisfy all of our stringent criteria (early exon, common exon, high on-target score, low predicted off-target score).

You can still go through design tool and order sgRNA "a la carte" then add-on Cas9 and a controls kit to essentially create your own GKO kit. As a little tip: since the guides found in the gene don't look to be optimal for knockout, you may consider multiplexing the guides (delivering 2 or 3 at a time to induce a small deletion). This will increase the likelihood of a knockout.