CCR5 (chemokine (C-C motif) receptor 5) also known as CD195 is a receptor for a number of C-C-chemokines, including MIP-1-alpha, MIP-1-beta and RANTES. CD195 is predominantly expressed on T cell, macrophages, dendritic cells and microglia. It is one of the major corecptors for HIV infectivity. CD195 is expressed at low density on activated cells. For the best immunofluorescent staining results, it may be best to maximise signal by using a sensitive three-layer staining technique or a bright fluorochrome for detection.

Catalog #

C2099-99G

Applications

Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution

Flow Cytometry: 1:10-1:20; 10ul labels 10e6 cells in 100ul

Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Clone Type

Monoclonal

Isotype

IgG

Clone No

10B939

Host

Hamster

Source

Mouse

Concentration

~0.5mg/ml

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity

Purified by immunoaffinity chromatography.

Immunogen

Mouse CCR5.

Specificity

Recognizes mouse CCR5.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.