Background & Aim: Tomor necrosis factor alpha (TNF-α; as a pro-inflammatory cytokine) and interlokin-10 (IL-10; asan anti-inflammatory cytokine) have an important role in the development and the prevention of systemic inflammation and incidence of obesity-induced diseases such as CAD, respectively. The aim of this study was to investigate the effectsof 8-week interval endurance combined training (IECT) on plasma TNF-α, IL-10, insulin resistance, and lipid profiles inoverweight and obese boy adolescents. Materials and Methods: In this semi-experimental study, 28 healthy overweightand obese boy adolescents (16.93 ± 1.89 yr., 88.07 ± 9.98 kg. and 28.35 ± 2.55 kg/m²) were volunteered and randomlyassigned into training (n= 13) and control (n= 15) groups. Exercise protocol was IECT (8 WK, 4 d/wk, 70 min/d). Bloodsamples were taken before and after exercise to assess the variables. Serum levels of TNF-α and IL-10 were measuredby sandwich ELISA method. T-test and Pearson correlation coefficient was employed. The criterion of statistical significance was set as p0.05). Moreover, the amounts of insulin resistance, triglyceride, total cholestrol; low density lipoprotein, low density lipoprotein/high density lipoprotein, total cholesterol/high density lipoprotein, body fat percent, body mass index, and waist circumference were significantly decreased and VO2max significantly increased (p<0.05).Conclusion: It seems that a combination of interval endurance training for 8 weeks can be considered to weight controling, reducing blood lipids and improving aerobic fitness, but to create favorable changes in cytokines, such as TNF-α and IL-10 is not sufficient. Probably, it is more useful with more intensity and volume. To achieve more accurate results, more studies are needed in this area.