Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Apaf1 can also associate with caspase-4 and caspase-8 (4). Apaf1 is ubiquitously expressed in human tissues (1).

Catalog #

A2298-65

This polyclonal antibody can be used for detection of Apaf1 by Western blot at 1

1000 to 1:2000 dilution. Whole cell lysate from HeLa cells can be used as positive control and a 130kD band should be detected. It is human and mouse reactive.

Western blot analysis of 50 ug of whole cell lysate from HeLa cells with anti-Apaf1 at 1

1000 dilution.

See corresponding Blocking Peptide for A2298-65P

Clone Type

Polyclonal

Isotype

IgG

Host

Rabbit

Source

Human

Concentration

0.5mg/ml

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

Purity

Purified IgG

Immunogen

Peptide corresponding to amino acids 12 to 28 of human Apaf1 (1). The sequences of the immunogenic peptide are identical between human and mouse (1,2)

Specificity

A 130kD band should be detected. It is human and mouse reactive.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.