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VECTORMUNE® FP MG is a genetically engineered live virus vaccine
for the vaccination of chickens as an aid in the prevention of fowl
pox and Mycoplasma gallisepticum. The fowl pox vaccine has been
genetically engineered to contain and express key protective
Mycoplasma gallisepticum bacterial antigens.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Production of medical or pharmaceutical compounds (human or animal) - Vaccines

Notes regarding the genetic elements introduced or modified in this LMO

A 3kb Hpal-Spel fragment of the FPV genome was inserted into a
EcoR1-HindIII site of a pUC18 vector. The homologous recombination
site may be interrupting a possible open reading frame but is
thought to be non-essential for viral replication and has no known
function in the FPV.

A 175bp EcoRV fragment of the FPV genome was removed from this
construct and replaced with the MG 40K and mcg3 coding
sequences.

Regulatory sequences were also inserted into the vector. The MG 40K
and mcg3 genes were each coupled with a synthetic Ps promoter,
which emulates the consensus early/late promoter of poxvirus. A
termination sequence derived from the gB gene of the Marek Disease
Virus was also inserted into the pUC18 vector.

Furthermore, a MDV gB transit signal sequence was added to the 3'
terminus of the of MG 40K and mcg3 coding sequences to ensure
localisation to the cell membrane.

This construct and the attenuated parental FPV strain were
co-transformed into chicken embryo fibroblasts (CEF) and incubated
under conditions that favour homologous recombination.

After transfection, viral particles were cultivated from the
chicken embryo fibroblasts and assayed for expression of MG 40K and
mcg3 proteins. Plaques expressing MG 40K and mcg3 proteins were
isolated and selected until the pure recombined virus was obtained.