概述

Abcam's Aspartate Assay Kit provides a simple, convenient assay to measure aspartate in a variety of samples. In the assay, aspartate is converted to pyruvate which is oxidized with the conversion of a probe into a highly colored (570 nm) and fluorescent (Ex/Em 535/587 nm) species proportional to the amount of aspartate in samples. Aspartate can be quantified in the range between 0.1–10 nmoles/well (2-200 µM). Visit our FAQs page for tips and troubleshooting.

说明

L-Aspartic acid (Asp) is one of the 20 proteinogenic amino acids as building block for protein. Although a non-essential amino acid in mammals, it is a precursor to several other amino acids including four essential amino acids (Met, Thr, Ile and Lys). Aspartate is a metabolite in the urea cycle, participates in gluconeogenesis and transports reducing equivalents between the cytosol and the mitochondria via the malate-aspartate shuttle. Aspartate also stimulates NMDA receptors (not as strongly as glutamate) and hence serves as an excitatory neurotransmitter in the brain and is an excitotoxin.

How many cells should I use for the assay? In which volume of Aspartate assay buffer should I resuspend them? - Start with ˜2x10^6 cells, suspend the cell pellet in 500 μl of the aspartate assay buffer on ice. Homogenize using a Dounce homogeni...

Start with ˜2X10^6 cells, suspend the cell pellet 500 μl of the aspartate assay buffer on ice, homogenize using a Dounce homogenizer (10-50 passes) on ice, until efficient lysis is confirmed, by viewing the cells under the microscope. Spin...

Thank you for your inquiry. I can confirm that all these kits are suitable for mouse samples: ab83362 and ab102512: These kits can be used with a variety of samples such as serum, plasma, and urine and should not be species dependent. As long as there ...