Target

Function

May contribute to the transparency and refractive index of the lens.

Tissue specificity

Lens as well as other tissues.

Involvement in disease

Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients.

Sequence similarities

Belongs to the small heat shock protein (HSP20) family.

Cellular localization

Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.

Images

Alpha B Crystallin was immunoprecipitated using 0.5mg Mouse Spinal Cord tissue, 5µg of Rabbit polyclonal to Alpha B Crystallin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

The antibody was incubated under agitation with Protein G beads for 10min, Mouse Spinal Cord tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab13497.

Thank you for your enquiry.
I can confirm that other than clotting being removed from whole blood (standard serum processing), there has been no additional purification to this product.
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