Data extracted from this reference:

Activating Compound

Activating Compound

Commentary

Organism

Structure

additional information

enzyme needs product, i.e. SldB, of upstream gene sldB, encoding a hydrophobic polypeptide, for activity, the polypeptide might be a chaperone-like component that signals processing and folding of the enzyme to give the active enzyme form

Gluconobacter oxydans

Cloned(Commentary)

Commentary

Organism

expression of wild-type and mutant enzymes in Escherichia coli

Gluconobacter oxydans

Engineering

Amino acid exchange

Commentary

Organism

additional information

construction of disruption mutants of genes sldA or sldB, the mutants are inactive, the sldB mutation leads to a higher enzyme expression and accumulation of unprocessed SldA

Gluconobacter oxydans

Localization

Localization

Commentary

Organism

GeneOntology No.

Textmining

membrane

bound

Gluconobacter oxydans

16020

-

Natural Substrates/ Products (Substrates)

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

D-sorbitol + NAD+

Gluconobacter oxydans

enzyme is the main L-sorbose producing activity in the cell

L-sorbose + NADH + H+

-

-

?

D-sorbitol + NAD+

Gluconobacter oxydans IFO 3255

enzyme is the main L-sorbose producing activity in the cell

L-sorbose + NADH + H+

-

-

?

Organism

Organism

Primary Accession No. (UniProt)

Commentary

Textmining

Gluconobacter oxydans

-

strain IFO 3255, gene sldA

-

Gluconobacter oxydans IFO 3255

-

strain IFO 3255, gene sldA

-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]

Specific Activity Maximum [µmol/min/mg]

Commentary

Organism

additional information

-

activity at growth in media with different pH values

Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

D-sorbitol + NAD+

-

655022

Gluconobacter oxydans

L-sorbose + NADH + H+

-

-

-

?

D-sorbitol + NAD+

enzyme is the main L-sorbose producing activity in the cell

655022

Gluconobacter oxydans

L-sorbose + NADH + H+

-

-

-

?

D-sorbitol + NAD+

-

655022

Gluconobacter oxydans IFO 3255

L-sorbose + NADH + H+

-

-

-

?

D-sorbitol + NAD+

enzyme is the main L-sorbose producing activity in the cell

655022

Gluconobacter oxydans IFO 3255

L-sorbose + NADH + H+

-

-

-

?

Temperature Optimum [°C]

Temperature Optimum [°C]

Temperature Optimum Maximum [°C]

Commentary

Organism

25

-

assay at

Gluconobacter oxydans

pH Optimum

pH Optimum Minimum

pH Optimum Maximum

Commentary

Organism

6

-

assay at

Gluconobacter oxydans

Activating Compound (protein specific)

Activating Compound

Commentary

Organism

Structure

additional information

enzyme needs product, i.e. SldB, of upstream gene sldB, encoding a hydrophobic polypeptide, for activity, the polypeptide might be a chaperone-like component that signals processing and folding of the enzyme to give the active enzyme form

Gluconobacter oxydans

Cloned(Commentary) (protein specific)

Commentary

Organism

expression of wild-type and mutant enzymes in Escherichia coli

Gluconobacter oxydans

Engineering (protein specific)

Amino acid exchange

Commentary

Organism

additional information

construction of disruption mutants of genes sldA or sldB, the mutants are inactive, the sldB mutation leads to a higher enzyme expression and accumulation of unprocessed SldA