Lentivirus Plasmids

ATCGbio Life Technology Inc. offers leniviral vector plasmids (pSLICs) that are based on the third generation lentivirus construct having only one internal promoter (CMV). SLIC (Sequence and Ligation Independent Cloning,Nature Methods 4:251-256, 2007) technology is used in these vectors. Our brand new SLIC enzyme allows to insert PCR product into the vector. In 30 min reaction, PCR product can be inserted in immediately after CMV site.

The bicistonic technique, a P2A motif system, is also used in pSLICs plasmids. Porcine Teschovirus-1 (PTV-1), a swine specific virus, contains 2A oligopepetides (P2A), a highly conserved c-terminal D(V/I)EXNPGP motif which allows the ribosome to recognise 2A "Self-Cleaving" between G and P site. pSLICs have been pre-made to express puromycin resistant gene and GFP respectively by "Self-Cleaving" with customer's target gene.

pSLICs allows user to create bicistronic (one promoter expressing two proteins) expression lentiviral vector plasmid with self-cleavage viral sequence (P2A like peptide) and SLIC technique. By this way, user can create lentivirus that can express two proteins (puromycin resistant or GFP with protein of interesting) equally.

For meeting customers’ demand, there are following two different configurations.

1) pSLIC-PuroP2ATM(LT2002) vector is constructed based on pL-SLIC-base vector adding puromycin resistant gene at downstream of CMV promoter followed by P2A sequence and EcoRV-flanked staffer sequence. P2A sequence allows to express both puromycin resistance gene and gene of interest inserted by SLIC, separately (bicistronic). This plasmid is suitable to make lentivirus for transformed or cancer cell lines in which cell division is not limited and easy to perform antibiotic selection.

2) pSLIC-GfpP2ATM (LT2003) vector is constructed based on pL-SLIC-base vector adding Gfp sequence at downstream of CMV promoter followed by P2A sequence and EcoRV-flanked staffer sequence. P2A sequence allows to express both GFP and gene of interest inserted by SLIC, separately (bicistronic). This plasmid is suitable to make lentivirus for primary cells in which antibiotic selection is difficult to perform but monitoring expression rate is important.