Data

Figure 2 shows the corrected UV-Vis Bradford Spectra for the post-dialysis solutions from the non-protein side of the dialysis chamber. From the negative absorbance values, it is clear that there is no protein present. This is expected since the dialysis tubing used had a molecular weight cut-off of 3500 g/mol.

Figure 3 shows the corrected UV-Vis Bradford Spectra for the post-dialysis solutions from the protein side of the dialysis chamber. It is clear from the spectra that there is still a lot of protein in each of the chambers, and the post-dialysis concentration of Lysozyme in solution almost perfectly matches the concentration of Lysozyme in the stock solution.

Figure 4 shows the calculations done to determine the concentration of Lysozyme in each solution. From these calculations, it would seem that the actual concentration of stock Lysozyme solution is .28 g/L rather than .5 g/L as believed. This is because the stock solution is diluted by a factor of 50 when it is mixed with Bradford reagent and buffer.