The authors conclude that H2S leads to hyperalgesia in diabetic rats through activation of TRPV1, TRPA1 and TRPC channels and, subsequent intraepidermal fibers loss. CBS enzyme inhibitors or TRP-channel blockers could be useful for the treatment of painful diabetic neuropathy.

Wednesday, January 09, 2019

Used to Study Apoptosis Induced by Brain Insults
Although our primary human cells are increasingly being used in drug discovery and toxicology assays versus animal cells, we are always pleased to see the success of the latter in sophisticated studies.

Highlights
Formyl-methionyl-leucyl-phenylalanine (fMLP) may be present in the brain in the course of some infectious diseases of the central nervous system (CNS), although little is known about its role. This investigation was performed to study the effect of fMLP on neuron apoptosis. The results showed that fMLP treatment of primary cultures of neurons was able to induce morphological features of apoptosis in cell cultures, as well as activation of the intrinsic apoptotic pathway, through the upregulation of caspase-9 and caspase-3.

The present study emphasizing the potential role of infectious agents, such as N-formyl peptides, in neurodegenerative diseases may help to promote the development of new therapies able to modulate the expression of the N-formyl peptide receptors.

Tuesday, January 01, 2019

Tri-culture of Neuromics' Human Primary Cells in 3-D BBB Model I mentioned in an earlier post that we take Researchers questioning the validity of our Human Primary Cells very seriously. We follow up with our clients to make sure our cells are working as expected. If they are not 100% happy, we offer a free replacement or full refund.

Given that these pericytes are also part of our hot selling 3-D Human BBB Model, we are in the process of challenging these claims. We will always take inaccurate claims regarding our solutions seriously, and we take immediate action.

Characterization of cells-we validated several key markers by immunofluorescence.

We also plan on doing a phenotypic analysis of these cells and will post here when completed.

Cost of cells-isolating pericytes from human donors, and expanding to the required number of cells involves much time and effort. We know! Further differentiating stem cells into pericytes is hard and time-consuming. Against this backdrop, we consider our pricing to be inexpensive. Though they are more difficult to derive, they are priced equivalently with our other human primary cells ($789/500,000 USD Cells).

I purchased FBS from Neuromics. It was shipped to Canada without any problems. It arrived safely and still frozen. Cells grow well in the product. Customer service was great and I will definitely shop here again.

Here's a model of the actual nerve receptors behind both intractable pain and loss of sensation.

A: Simplified model of nociception under normal conditions. Free nerve endings transduce a painful stimulus into a neural signal, which propagates to DRG centrally and eventually synapses on a nociceptive neuron within the DH of the spinal cord.B: Proposed model of nociception under conditions of diabetic hyperalgesia and allodynia. A pain signal augmented by upregulated pronociceptive ion channels in sensory neurons is carried toward the DH, where it is further augmented by a hypoactive GABAergic system and subsequently diminished inhibition from an inhibitory interneuron. NMDAR, N-Methyl-D-aspartate receptors. https://doi.org/10.2337/dbi15-0006

We will continue to post findings on the root causes and potential treatment for Neuropathy.

These cells are optimized to provide additional options for in-vitro testing of drug to drug candidate toxicities allowing researchers to rule out the ineffective and potentially toxic small molecules/compounds early in the process.

Immunofluorescent analysis of rat brainstem section stained with GT22102, MAP2 dilution 1:2,000 in red, and costained with mouse mAb to MO22121, MBP dilution 1:5,000, in green. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was postfixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies.

If you have questions on these or any of our solutions, not hesitate to contact me directly-pshuster@neuromics.com or 612-801-1007,

Sunday, September 30, 2018

First Come; First Serve
We have an inventory of FBS of approximately 2000 bottles. This inventory includes the lots we've been selling and using internally all year. These have proven to be of the highest quality.
Here’s a sampling of user feedback:

After a referral from an ex-colleague, we tested a sample from a single batch and found that the product is great for cell cloning & hybridoma work, and generally for all other cell culture. We subsequently ordered several bottles of FBS. The sales person was very friendly and worked with me to make the purchase possible. The order arrived promptly. - Peter Dias, The Biomedical Research Institute of SC

We ordered several bottles of Fetal Bovine Serum on 2 occasions and are pleased with its performance in our cultures. The FBS arrived frozen in enough dry ice and was neatly and carefully packed. The FBS was very reasonably priced. Thank you. - Ken Patrene, University of Pittsburgh

N39 Cell Line Cells grown in Media supplemented with Neuromics' FBS, Courtesy of Deng Guo, University of Iowa.HEK Cells cultured in Media Supplemented with Neuromics' FBS Courtesy of Kavita Shah, Purdue University

The reason for this great offer is we need to clear inventory so that we can place a volume order of raw FBS stock yet this year and lock in 2019 pricing. Our goal is to provide the best FBS at the lowest price. This offer of $259/500ml is valid until the current stock is exhausted.
Like a sample to try? Contact rose@neuromics.com for a 50 ml sample. Rose Ludescher-Manager of Customer Satisfaction-

GREM-1 is required for tube formation in vitro(A) Q-RT-PCR analysis of GREM-1 gene expression in SU-DIPG-IV cells stably expressing either control shRNA or GREM-1-specific shRNA. Lentiviral constructs expressing two different GREM-1 shRNAs (GREM-1.1 and GREM-1.2) were used to knockdown GREM-1. Efficiency of GREM-1 knockdown was determined by Q-RT-PCR and expression was normalized to 18s RNA. Significance is as shown (**less than.01). (B-C) HUVEC or human brain microvascular endothelial cells (HBMEC) were cultured in endothelial cell medium and or conditioned medium from either control shRNA or shGREM-1.2 transfected SU-DIPG-IV cells. Tube formation in matrigel was measured after 16h and images were obtained. The ability of GREM-1 to rescue loss of tube formation upon REST knockdown was determined by addition of human-recombinant GREM-1 (rGREM-1) to conditioned media-endothelial media mix. Scale bars, 100μm. (C) Quantification of tubes in matrigel shown in Figure B (right panels). Data shown is mean +/- SD, ***p less than .001, n=3. (D) Western blot analysis to assess VEGFR2 levels in SU-DIPG-IV, -VI and –XIII cells, HUVECs and HBMECs was done using anti-VEGFR2 antibodies. Tubulin served as a loading control. (E) Western blot analysis was performed to assess AKT signaling downstream of GREM-1 interaction with its potential receptor VEGFR2 in HUVEC and HBMEC. Anti-pAKT (S473), anti-pAKT (T308), total AKT, and anti-actin were employed.

If these cells fit your assay requirements and need more data/info, do not hesitate to contact me, Pete Shuster, CEO at pshuster@neuromics.com or cell: 612-801-1007.

Friday, September 14, 2018

Crossing the Blood-Brain Barrier
Our BBB Model is being increasingly used by Bio-Pharma for drug permeability studies. Customers include Amgen, Genentech, Boehringer Ingelheim, and Merck.

These assays are key for making sure molecules/compounds of interest will cross the barrier into the brain and at what rate. This data, in part, add clarity on best candidates to move into in-vivo testing.

Monday, August 27, 2018

Check out our AlphaBioCoat
Collagen is a fibrous protein found in the extracellular matrix and connective tissue. The most common form of collagen is type I and is most prevalent in bone, tendon and skin. It consists of 3 intertwined coiled subunits: 2 x α1 (I) chains and 1 x α2 (I) chain. Each chain contains 1050 amino acids wound tightly around one another in a characteristic right-handed triple helix. The triple-helical structure of collagen arises from unique abundance of the amino acids in collagen appear in a characteristic repeating motif Gly-X-Y, where X is usually proline and Y is usually hydroxyproline.

AlphaBioCoat Solution (AC001) is a biocompatible complex of extracellular matrix binding solution that is supplemented with growth factors. It helps accelerate cell attachment and cell growth. AC001 is the premium version of our Smooth Coat Solution (SC300).

It is ideal for plate coating due to its unique viscosity. Its coating greatly enables cell migration on cultured plate surfaces. Perfect for establishing primary cell lines, it can increase endothelial cell attachment, survival in culture, and cell growth. AlphaBioCoat Solution is great for both coating plates and T-flasks.

Thursday, August 16, 2018

Our Expertise
When researchers are considering using our human brain cells, they often voice concerns about growth. In order to address these concerns, we explain that we have optimized our media and coatings in order to meet their assay needs.

Tuesday, July 24, 2018

Synaptic Plasticity in the Ear
Our mission to Mars is driving a need to better understand the physiological impact on the human body. Deep space travel places unique challenges for humans. It is important that there is minimal impact on space travelers' senses. This includes hearing.

This study uses our Shank 1a Antibody to determine changes in the neuronal structure of the ear in microgravity.

Image: Verification of antibodies to CtBP2 and Shank1a. A and B: serial 14-µm cryosections were obtained from a postnatal day 71 (P71) mouse utricle, and maximum-intensity projections are shown. Hair cell (hc) and support cell (sc) nuclei are illuminated by the DAPI stain (blue). Numerous closely associated CtBP2-and Shank1a-positive puncta can be observed in the positive immunostained section represented in A (block arrowheads). The CtBP2-positive puncta highlighted by the flared arrowhead in A may represent an undocked synaptic ribbon. No primary antibodies were included in the processing represented in the micrograph in B. The scale bar in B represents 5 µm and also applies to A. C and D: maximum-intensity projection micrographs from right and left whole mount utricles from a P65 mouse. Importantly, fixative administration into the temporal bones yielding the specimens represented in C and D was delayed 7 min to replicate the conditions associated with specimens derived from the microgravity and control specimens. The positive-immunostained specimen of the pair is shown in C, where numerous closely associated CtBP2-positive and Shank1a-positive puncta can be observed. Though faint, CtBP2-immunostained nuclei are highlighted by the flared arrowheads. The micrograph in D illustrates the results of withholding primary antibodies from the processing. Immunolabeled puncta are not observed. The scale bar in D represents 5 µm and also applies to C. https://www.physiology.org/doi/full/10.1152/jn.00240.2016

These results demonstrate that structural plasticity was topographically localized to the utricular region that encodes very low frequency and static changes in linear acceleration, and illuminates the remarkable capabilities of utricular hair cells for synaptic plasticity in adapting to novel gravitational environments.

Wednesday, July 18, 2018

The background is Bad!
Yes, it is. It compromises data. We have a solution.FluoMuteTM

FluoMute™ ready-to-use reagent to reduce autofluorescence in cells and tissue. Just incubate fixed cells of tissue sections with FluoMute™ for 30-60 min at room temperature, rinse with PBS and continue with immunofluorescence ICC or/and IHC protocols. Treatment with FluoMute™ does not affect cell morphology and the integrity of tissue antigens to be detected with primary antibodies (see bottom image). FluoMute™ is compatible with paraffin-embedded and frozen tissue sections, stem cells, lymphocytes and mammalian cell lines of different origin.

Wednesday, July 11, 2018

Human Microvascular Retinal Endothelial Cells (HMRECS)
We have built the foundation of Neuromics on satisfied customers. We make a practice of following up with each user to make sure our solutions are working as expected. If not, we offer "no question asked" refunds or replacements.

This is especially important for our human cells, media, and supplements. Success with these is easy to measure as either the cells are healthy and happy or they are not.

Monday, July 02, 2018

Potent FBS at Pricing You'll Like
Neuromics started providing FBS to researchers in early 2017. Our goal was to provide thorough tested and 9-CFR compliant FBS with the lowest pricing anywhere.

In order to ensure our initial claims are trustworthy, we follow up with all our users and ask that they provide feedback. Here's the latest review-Ordered Fetal bovine serum. Best price and quality" - Juwen D, Albert Einstein College of Medicine. Product: Heat-inactivated FBS, cat no. FBS001-HI.

Thursday, June 28, 2018

Check out Testimonials from Users
Neuromics is proud to offer you the high-quality Fetal Bovine Serum, the same serum we use internally, without the high cost. Our customers, researchers like you, agree.Ordered Fetal bovine serum. Best price and quality" - Juwen D, Albert Einstein College of Medicine. Product: Heat-inactivated FBS, cat no. FBS001-HIOutstanding service. Fetal bovine serum was of highest quality, easy to order and packaging and shipping were best possible." - Rosemary, University of Buffalo Product: Heat-inactivated FBS, cat no. FBS001-HI (see all of Neuromics reviews here).

Give our serum a try today! Neuromics US-Origin Fetal Bovine Serum is only 349USD/500ml. Heat-inactivated FBS only 364USD/500ml. Need a large quantity? Bulk discounts are available. Like a sample to try? Contact rose@neuromics.com for a 50 ml sample.

Tuesday, June 26, 2018

Detects Autophagy in Living Cells
Autophagy is a conserved lysosomal recycling process by which cells break down their own components such as proteins, lipids, and carbohydrates. The process plays an important role in maintaining homeostasis and destroying intracellular pathogens. In addition, autophagy can be upregulated in times of starvation or stress to provide additional nutrients for the cell. Dysregulation of autophagy has implications for cancer, infection, and degenerative diseases.

Autophagy is a three-stage process. First, cytoplasmic components targeted for degradation are sequestered, resulting in the formation of the autophagosome. Next, the autophagosome fuses with the lysosome to form the autophagolysosome or autolysosome. Finally, degradation of the autophagosomal contents occurs.

Our Autophagy Assay, Red (cat# KF17373) enables researchers to detect and monitor the in vitro development of autophagy in living cells. The Autophagy Probe is cell-permeant and fluoresces red when inserted in the lipid membranes of autophagosomes and autolysosomes. Results can be read using a flow cytometer.

Figure. Flow Cytometry Results. Autophagy Assay Kit, Red was used to assess the induction of autophagy in Jurkat cells. Cells were either untreated (Black) or treated with 0.5 μM Rapamycin (Orange), 10 μM Chloroquine (Blue), or both 0.5 μM Rapamycin and 10 μM Chloroquine (Red) for 18 hours. After staining with Autophagy Probe, Red for 60 minutes, cells were washed and analyzed by flow cytometry (BD LSRFortessa Special Order flow cytometer equipped with a green/yellow laser (561 nm excitation) and a 610/20 emission filter). An overlay of the histograms is shown on the right. A table displaying the median fluorescence signal, % negative, and % positive cells is shown below. Treatment with Rapamycin or chloroquine increased the fluorescence signal detected compared to the untreated control. Combined treatment of rapamycin and chloroquine further increased the fluorescence signal detected. Data courtesy of Dr. Kristi Strandberg (ICT 228:37-40).

Wednesday, June 06, 2018

Tested and Characterized for Results You Can Trust
When you select a vendor for antibodies, there is a measure of trust. We know you are asking, "will it work in the applications as advertised".

Since the inception of Neuromics, our guiding principle is providing well-characterized solutions for results you can trust and understand. In order to provide as much comfort in the purchase of our solutions, we encourage access to publications, data, and testimonials.

Neuromics has a conjugated antibody for oligodendrocytes that is unavailable anywhere else and works really well. Very pleased with the product and have already ordered it a few times. Edit: This antibody is available from Miltenyi now. They both behave comparably. Matthew Smith - Apr 24, 2018 - Rating: 5.0

I bought the secondary antibodies (488-Goat anti mouse IgG and 546-goat anti rabbit IgG) from Neuromics and used them for IF assay. Both antibodies worked well. I highly recommend them.Bonnie Dai - Apr 21, 2018 - Rating: 5.0

Nop1p/Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli. Nop1p is the yeast homolog of a protein apparently found in all eukaryotes and archaea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6). The HGNC name for this protein is FBL. To raise the MCA-4H4 antibody, mice were injected with full length recombinant human fibrillarin.

Wednesday, May 30, 2018

More in-vivo like Model
We see our world in 3-D. Diseases of the eye compromise this ability.

Neuromics' is pleased to announce that we have a 3-D model aimed at accelerating drug discovery for these diseases. Sight is a terrible thing to lose and the faster new drugs can be discovered, fewer people will have to suffer the loss of sight.

Thursday, May 10, 2018

Potent Serum at a Great Price!
We are pleased by the 5-star ratings on our Fetal Bovine Serum (FBS). Check them out.

Outstanding service. Fetal bovine serum was of highest quality, easy to order and packaging and shipping were best possible.Rosemary Dziak - May 08, 2018 - Rating: 5.0

This is first time to buy FBS from them. Rose is very nice person. they make you easy and comfortable to purchase.jie wei - Feb 16, 2018 - Rating: 5.0

I enjoyed working with the people at Neuromics. The sale person was very friend and willing to take extra time to solve our problems. Product Name: FBS - (Cat#FBS001) https://www.neuromics.com/FBS001 Organization: MayoWenqian Hu - Oct 15, 2017 - Rating: 5.0

Our FBS is 9CFR-tested, meeting FDA and USDA requirements. Fetal bovine serum products can also be tested to meet EMEA requirements. Note: We test each lot of FBS on our primary human cell cultures enabling us to choose lots yielding the best results...
Testimonial: "We have used the FBS from Neuromics in feeding media for primary mouse astrocytes as well as for some cell lines. Your product is good and we plan to continue using it.” - Svetlana Vidensky, MS, Senior Research Specialist in Dr. Jeffrey Rothstein lab, Department of Neurology, Johns Hopkins University Contact Rose at rose@neuromics.com or 952-374-6161 for bulk pricing.

FIGURE 1. A schematic of the in vitro transmembrane pressure device. (a) Fibroblasts were grown as a monolayer on the transmembrane of a 0.4 lm transwell insert and a piston of adjustable weight was applying a compressive stress. Control cells were covered with an agarose cushion only. (b) The experimental set-up of the co-culture system consisted of fibroblasts and pancreatic cancer cells (MIA PaCa-2 or CFPAC-1) in the upper and lower chamber of a transwell insert, respectively. A piston with adjustable weight, applying 4.0 mmHg of compressive stress on fibroblasts for 48 h is shown. A co-culture system consisting of fibroblasts and cancer cells without a compressive load was used as a control.

FIGURE 1. A schematic of the in vitro transmembrane pressure device. (a) Fibroblasts were grown as a monolayer on the transmembrane of a 0.4 lm transwell insert and a piston of adjustable weight was applying a compressive stress. Control cells were covered with an agarose cushion only. (b) The experimental set-up of the co-culture system consisted of fibroblasts and pancreatic cancer cells (MIA PaCa-2 or CFPAC-1) in the upper and lower chamber of a transwell insert, respectively. A piston with adjustable weight, applying 4.0 mmHg of compressive stress on fibroblasts for 48 h is shown. A co-culture system consisting of fibroblasts and cancer cells without a compressive load was used as a control.

Solid stress developed within tumors is able by itself to activate normal ﬁbroblasts, which in turn produce excessive amounts of ECM proteins leading to desmoplasia.