Working Giemsa Solution may be made from the stock solution (#26132-01) by mixing
- Giemsa Stock Solution - 1.25ml
- Methanol - 1.5ml
- Distilled Water - 50.0ml
NOTE: Giemsa stain colors more effectively in tissue at an acid pH. If this has not occurred in the preparation or decalcification steps, wash in acid alcohol, then begin stain

Differentiate in Rosin Alcohol Working solution (#26132-2A) until the sections are purplish-pink color. Check under microscope.

Dehydrate in two changes of absolute alcohol and clear in two changes of xylene.

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Electron Microscopy Sciences specializes in the manufacturing, preparation and distribution
of the highest quality laboratory chemicals and microscopy supplies and equipment
for electron microscopy, light microscopy, and histology.