Will my data remain the same if I switch from SsoAdvanced SYBR Green supermix to SsoAdvanced univ

Will my data remain the same if I switch from SsoAdvanced™ SYBR® Green supermix to SsoAdvanced™ universal SYBR® Green supermix?

Answer:

An integral requirement of the reformulation was to ensure that no significant changes were noted in performance. The Bio-Rad R&D team validated these updated supermixes with an extensive set of qPCR assays to assess the following attributes:

In terms of absolute Cq values, although the vast majority of data were identical to those obtained with the previous formulation, we noted a few assays that demonstrated Cq shifts of ±0.5. This shift does not impact qPCR data in any manner, as Cq values in and of themselves are meaningless values.

See Normalization of Real-Time PCR Fluorescence Data with ROX Passive Reference Dye in our Applications and Technologies pages to learn why passive reference dyes are required for data normalization in some real-time PCR systems and not others. Although Bio-Rad real-time PCR detection systems do not require ROX for internal normalization, Bio-Rad’s universal real-time PCR supermixes are designed for high performance on all real-time PCR detection systems, both systems requiring ROX normalization and ROX-independent systems.

I cannot find the Certificate of Analysis I am looking for. Help!

When using our online Certificate of Analysis search tool, keep the following in mind:

Make sure you entered the correct catalog number and lot/control number in the search fields

If you have a kit, try looking up the Certificate of Analysis using the kit information as well as the information for the individual components

The Certificate of Analysis that you are looking for may not be available via the website. In that case, you can contact your Bio-Rad representative or use the Request Form

If you cannot find the Certificate of Analysis you are looking for, please Contact Us.

Where can I find the catalog number, SKU number, or product number?

The catalog number, SKU number, or product number is printed on your product label. See the sample label below for the location of this information.

Where can I find the lot or control number?

The lot number or control number (one or the other, not both) is printed on your product label. See the sample label below for the location of this information.

Why are Certificates of Analysis not on the Documents tab?

Certificates of Analysis are associated not only with a product, but also with a specific lot of that product. For a given product, there may be several Certificates of Analysis, especially if it is an older product line and has had several lots produced over the years. Using the Certificate of Analysis search tool, you can enter the catalog number and lot/control number for the specific product you have on hand and download the exact Certificate of Analysis you need.

I have a batch number, not a lot/control number. How do I find the Certificate of Analysis for my item?

You can use the batch number in place of the lot/control number. Use the Certificate of Analysis search tool, entering the catalog number as usual and the batch number where the lot/control number would go.

Can I get a Certificate of Analysis even if my item is expired?

Yes. While we do periodically remove Certificates of Analysis for site maintenance purposes, we typically keep them available well beyond a product's expiration date.

Why does it say "Product Name Not Found" for a Certificate of Analysis listed in the search results?

There are Certificates of Analysis that correspond to products that are either discontinued or simply not available on the website. In these situations, the Certificate of Analysis is available for download, but other product details, such as the product name, are not.

Why does it say "Not Available" for a Certificate of Analysis listed in the search results?

In this case, you have entered a valid catalog number and lot/control number and we have located the Certificate of Analysis. However, for some reason, the actual file itself is unavailable.

Please use the Request Form here or contact your local Bio-Rad Representative so that we can send you the Certificate of Analysis.

All Bio-Rad products are guaranteed to meet the specifications listed in our catalog. Should a Bio-Rad branded product fail to meet specifications during its warranty period, it shall be repaired or replaced at Bio-Rad's discretion. Warranty periods are listed on quotations for specific products; however, instruments generally receive 1 year of warranty coverage from the date of shipment or installation. Instrument warranties do not include consumable parts, such as lamps, lasers, and platinum wire. Repairs have different warranty periods. Chemicals and other consumables are warranted through their expiration date, or for 1 year from shipment if no expiration date is indicated. Non-Bio-Rad branded products, such as computers and computer peripherals, are covered by the original equipment manufacturer's warranty. Warranties are not transferable from the original purchaser. Warranty coverage may be void if the product is moved outside of the country where the product was originally purchased. An optional extended warranty program is available. For information, call 1-800-4BIORAD, and select Inside Sales.

Your subscription information already exists, we will send you an email with specific instructions to manage your existing subscription profile.

To receive the latest news, promotions, and more, sign up for Bio-Rad updates by entering your email address below. You can elect to receive only the types of Bio-Rad communications that are of interest to you..