YPDA & YPD Rich Media

YPD and YPDA yeast media pouches provide media aliquoted in highly convenient pouches. Simply empty the contents of each into your media flask/bottle, add 0.5 L of dH2O, and sterilize. No weighing of individual supplements, combining, or pH adjustment is required. All media pouch mixes are available either with agar or without (broth).

Bulk Media Format

YPD and YPDA media for yeast are also available in bulk format with agar (700 g) or without agar (500 g).

YPD Medium

YPD Medium is a blend of peptone, yeast extract, and dextrose in optimal proportions for routine subculture of Saccharomyces cerevisiae. This medium is available with or without agar premixed in the medium.

YPDA Medium

YPDA is the same as YPD but with the addition of an adenine hemisulfate supplement to a final 1X concentration of 120 mg/L. The adenine supplement prevents yeast strains harboring ADE1 and ADE2 mutations (e.g. Y2HGold, AH109, and Y187) from turning pink/red after extended growth.

Applications

For routine subculturing of untransformed yeast

Yeast two-hybrid screening

Yeast one-hybrid screening

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.