Reading error with deconvolution

Reading error with deconvolution

Greetings,

My lab is looking to get into using imagej (specifically for vamping) and
are excited for the possibilities it can provide us, but we seem to
encounter an error/exception when we try to open deconvoluted image-stacks.
We can view our images fine prior to deconvolution and can use vamping for
them, but we're hoping to try to run through the vamping process with a 3D
convolution using AutoQuant.

The error/exception reads as:

java.lang.ClassCastException: [F cannot be cast to [S
at Ics_Opener$IcsImport.OpenIcs(Ics_Opener.java:378)
at Ics_Opener.open(Ics_Opener.java:91)
at Ics_Opener.run(Ics_Opener.java:34)
at ij.IJ.runUserPlugIn(IJ.java:183)
at ij.IJ.runPlugIn(IJ.java:150)
at ij.IJ.runPlugIn(IJ.java:139)
at HandleExtraFileTypes.tryPlugIn(HandleExtraFileTypes.java:278)
at HandleExtraFileTypes.tryOpen(HandleExtraFileTypes.java:140)
at HandleExtraFileTypes.openImage(HandleExtraFileTypes.java:242)
at HandleExtraFileTypes.run(HandleExtraFileTypes.java:37)
at ij.IJ.runUserPlugIn(IJ.java:183)
at ij.IJ.runPlugIn(IJ.java:150)
at ij.IJ.runPlugIn(IJ.java:139)
at ij.io.Opener.openWithHandleExtraFileTypes(Opener.java:410)
at ij.io.Opener.openImage(Opener.java:287)
at ij.plugin.FolderOpener.run(FolderOpener.java:175)
at ij.IJ.runPlugIn(IJ.java:156)
at ij.Executer.runCommand(Executer.java:127)
at ij.Executer.run(Executer.java:64)
at java.lang.Thread.run(Thread.java:619)

> Greetings,
>
> My lab is looking to get into using imagej (specifically for vamping) and
> are excited for the possibilities it can provide us, but we seem to
> encounter an error/exception when we try to open deconvoluted image-stacks.
> We can view our images fine prior to deconvolution and can use vamping for
> them, but we're hoping to try to run through the vamping process with a 3D
> convolution using AutoQuant.
>
> The error/exception reads as:
>
> java.lang.ClassCastException: [F cannot be cast to [S
> at Ics_Opener$IcsImport.OpenIcs(Ics_Opener.java:378)
> at Ics_Opener.open(Ics_Opener.java:91)
> at Ics_Opener.run(Ics_Opener.java:34)
> at ij.IJ.runUserPlugIn(IJ.java:183)
> at ij.IJ.runPlugIn(IJ.java:150)
> at ij.IJ.runPlugIn(IJ.java:139)
> at HandleExtraFileTypes.tryPlugIn(HandleExtraFileTypes.java:278)
> at HandleExtraFileTypes.tryOpen(HandleExtraFileTypes.java:140)
> at HandleExtraFileTypes.openImage(HandleExtraFileTypes.java:242)
> at HandleExtraFileTypes.run(HandleExtraFileTypes.java:37)
> at ij.IJ.runUserPlugIn(IJ.java:183)
> at ij.IJ.runPlugIn(IJ.java:150)
> at ij.IJ.runPlugIn(IJ.java:139)
> at ij.io.Opener.openWithHandleExtraFileTypes(Opener.java:410)
> at ij.io.Opener.openImage(Opener.java:287)
> at ij.plugin.FolderOpener.run(FolderOpener.java:175)
> at ij.IJ.runPlugIn(IJ.java:156)
> at ij.Executer.runCommand(Executer.java:127)
> at ij.Executer.run(Executer.java:64)
> at java.lang.Thread.run(Thread.java:619)
>
> Thank you very much for your time.
>
> -Chris
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html>

Re: Reading error with deconvolution

Hi Curtis,

I downloaded that plugin and can open the files perfectly now, thank you
very much. However, now I'm having some trouble with the vamping plugin in
trying to run the photos through their algorithm. I'm going to look into
saving files in a 16-bit format.