In article <9408161553.AA13566 at post.its.mcw.edu>, FRED at bmt.mcw.edu ("Frederick Garbrecht") says:
>>A neophyte (me) needs help. When I am purifying genomic DNA from
>human lymphoid cells, I have a lot of trouble in the
>phenol-chloroform extractions - when I pipette off the upper aqueous
>phase, the DNA strands always seem to be attached to the junk at the
>interphase, so consequently I pull up a lot of the crap in the
>interphase and can't seem to get rid of it. Any suggestions? Do
You may try decrease DNA concentration (increase sample volume)
>people just take off half of the aqueous phase and sacrifice the rest
>to get a clean preparation? Thanks much --
Well, if you really want clean prep you must sacrifice some...
>>Frederick C. Garbrecht
>fred at bmt.mcw.edu>fgarbrec at post.its.mcw.edu>Bone Marrow Transplant Program
>Medical College of Wisconsin
>phone 414 257 5053
>fax 414 257 7994
Artur Burzynski, PhD
Vanderbilt University