In a final attempt to save his life, Charlie had started a trial of the drug Aurora Kinase in December.

The revolutionary drug, which is
administered over 72 hours every 21 days via intravenous infusion, is
intended to inhibit cancer growth by blocking the enzymes which cause
cancerous cells to grow.

But just weeks later, his parents announced the devastating news that the potentially lifesaving drug had failed to save him.

Side-by-side: Two-year-old Charlie was there for his father Joe as best man at his wedding throughout the day

Today, followers took to his Facebook page to pay tribute to Charlie.

Paula Gouldstone wrote: 'Rest In Peace Charlie, such a brave little boy.
My heart bleeds for all his family and friends. Cant imagine how they
are all coping right now, Such a shame. Sleep tight little angel xxxx'

Tanya Cunningham added: 'Night night Charlie Bear - you and your family
have touched the hearts of so many and you'll never be forgotten - Rest
In Peace little one xxxx'

Earlier today a picture had been posted showing a frail looking Charlie being cradled one last time by his big sister.

His heartbroken mother Fiona posted:
'Ellie loved her picture with Charlie yesterday [Thursday], she wanted
to show the whole world how much she loves him.

"However,
until Charlie is feeling stronger that will be his last picture, we
know it can be upsetting seeing someone so poorly and don't want to
offend anyone but it was important for Ellie to show you all how much
she loves her brother.'