Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LS-F3456 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat Insulin in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of Insulin as low as 4.688 micro international units per milliliter.

Custom Kits:

All LSBio Custom kits have been predesigned and all necessary components, including antibodies and standards have been identified. Upon receiving a custom kit order, the kit will be assembled and quality control tested before being shipped out. Kit assembly and testing typically takes 4 to 6 weeks. In most cases the final kit is based on the Sandwich assay principle, with a few being Competitive EIA based. Specifications such as Range, Sensitivity, and Precision are defined upon completion. In the event that the custom kit cannot be successfully developed with 6 weeks of the order date, the customer will be notified and the order canceled at no cost.

Sample Type:

Cell Culture Supernatants, Plasma, Serum

Sample Dilution:

2 fold

Format:

96-Well Microplate

Detection:

Colorimetric - 450nm

Measurement:

Quantitative

Detection Range:

4.688 - 150 micro IU/ml

Sensitivity:

4.688 micro IU/ml

Precision:

Intra-Assay: CV<10% Inter-Assay: CV<12%

Quality Assurance:

Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping. ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Kit Components:

Coated 96-well Strip Plate

Wash Buffer Concentrate (20X)

Standard (Lyophilized)

Biotinylated Detection Antibody

HRP-Streptavidin Conjugate (400x)

TMB One-Step Substrate Reagent

Stop Solution

Assay Diluent C

Assay Diluent B (5x)

Adhesive Plate Sealers

Storage:

May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Restrictions:

For research use only.

About Insulin

Description: After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified.