DEAE-transfection of EL-4 cells

hyho at GATE.SINICA.EDU.TW (Hung-yiu Ho) wrote:
>Dear netter,
> What is the optimal condition (i.e. DEAE-dextran concentration and
>duration of DMSO shock) for EL-4 cells? I have tried very hard to test
>it. Please help me!
The best source I know is Tom Novak, who worked very hard as a grad.
student at Caltech to optimize the conditions for these assays. He's
now at Wyeth-Ayerst, and his e-mail address is
novakt at prince.mm.wyeth.com
As far as I know, he's the best! He published this work in Mol. Cell.
Biol. Ask him for the reference.
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