SiliaBond Normal Phases

In normal phase chromatography, the packing material is always polar while the mobile phase is non polar. The interactions between analytes and sorbent mainly take place on the highly polar silanols of the silica gel surface. Some hydrogen bonds can also happen on polar functionalized groups.

SiliaBond Cyano (Si-CN) can be used both in normal and reversed-phase chromatography as its polarity marks the separation between the polar and non-polar phases. SiliaBond Cyano is the least retentive polar sorbent in normal phase chromatography and the least retentive of the non-polar sorbents in reversed-phase chromatography.

SiliaBond Cyano (Si-CN) can be used both in normal and reversed-phase chromatography as its polarity marks the separation between the polar and non-polar phases. SiliaBond Cyano is the least retentive polar sorbent in normal phase chromatography and the least retentive of the non-polar sorbents in reversed-phase chromatography.

SiliaBond Diol may also be used as polar sorbent in normal phase and aqueous size exclustion chromatography. Like bare silica, SiliaBond Diol has the ability to form hydrogen bonds and the capacity to separate structural isomers. Since most of its surface is covered with organic functions, the SiliaBond Diol absorbs less water, which leads to a more reproducible activity. It is also the sorbent of choice when working in normal phase in the presence of water.

Chromatography with silver nitrate is a well known and established methodology for the separation of non polar compounds with similar polarities. Generally, with a mixture of unsaturated compounds such as alkenes, lipids, steroids, terpenes, etc, standard separation procedures lack in efficiency. However, by using SiliaBond® Silver Nitrate for your column chromatography along with SiliaPlate™ Ag for your TLC, you will have the necessary tools to circumvent the problem.

SiliaBond Amine (Si-NH2) is an effective scavenger of acid chlorides, sulfonyl chlorides, isocyanates and other electrophiles. Si-NH2 has been shown to be effective metal scavenger and catalyst for Knoevenagel reactions. SiliaBond Amine is also used in chromatography as normal phase sorbent.

SiliaBond Amine (Si-NH2) is an effective scavenger of acid chlorides, sulfonyl chlorides, isocyanates and other electrophiles. Si-NH2 has been shown to be effective metal scavenger and catalyst for Knoevenagel reactions. SiliaBond Amine is also used in chromatography as normal phase sorbent.