Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2-phospho-glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (a, b and g). The alpha-subunit is expressed in most tissues and the beta-subunit only in muscle. The gamma-subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.

May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Clone Type

Polyclonal

Isotype

IgG

Host

Rabbit

Source

Human

Concentration

~0.1mg/ml

Form

Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide, before the addition of glycerol to 40%. .