Results

Looks like the elongation factor (EF1) primers and BB16 gDNA as a positive control are the way to go.

In the plots below, the black lines are BB16, the green lines are BB15, and the red lines are no template controls (NTC).

The amplification plots show that the EF1 primers do not amplify with BB15, but do amplify with BB16 (black lines Cq ~34). The 18s primers amplify with both BB15 & BB16 (Cq ~16 & ~18, respecitively), but produce primer dimers (red lines in amplification and melt curve plots).