Overview

SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

Goat Anti-Rabbit IgG H&L (FITC) images

ab84235 staining melanoma inhibitory activity in a human melanoma cell line by Flow Cytometry. Cells were harvested with EDTA and washed in PBS. Cells were pemeabilized with saponine. The sample was incubated with the primary antibody (1/100 in PBS) for 15 minutes at 20°C. A FITC-conjugated goat anti-rabbit IgG H&L (ab97050) (1/100) was used as the secondary antibody.

The FACS staining was perform on THP-1 cell lines with Rabbit polyclonal to CCR2 (ab21667) at a 1/100 dillution for 30 min at 4C. The secondary antibody was ab97050 used at 1/100 for 20min at 4C. The buffer used was PBS/BSA (0.5%)/Azide (0.05%). No fixation or permeabilization was performed and gating was done on alive cells.

The FACS staining was performed on HEK cells expressing human CCR5 with PBS/BSA (0.5%)/Azide (0.05%) as FACS buffer.
We used a primary polyclonal rabbit antibody against CCR5 at a 1/100 dillution, incubated for 1 hour at 4C.
The secondary antibody was ab97050 used at a concentration of 1/100 for 30min at 4C. No fixation or permeabilization was performed and gaiting was done on alive cells.

Thank you for contacting us. According to the datasheet, the this protein is not expressed in most lymphoid cell lines: B-cells, T-cells and NK cells. Therefore, I would suggest to use a T-cell line like Jurkat lysate as a negative control. ...

Thank you for your email and interest in Abcam products.
We currently do not have AlexaFluor conjugated antibodies or proteins available in our catalog. Having said this though, we have a number of products available that are conjugated to DyLight® ...