Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

prostate neuroendocrine cancer

Age

6 months

Gender

male

Applications

PNEC cell lines should be useful for genetic and/or pharmacologic studies of the regulation of NE cell proliferation, differentiation, and tumorigenesis.

GeneChip analyses of cell lines harvested at different passages, and as xenografted tumors, indicated that PNECs express consistent features ex vivo and in vivo and share a remarkable degree of similarity with primary CR2-TAg prostate neuroendocrine (NE) tumors.

PNEC30 cells, when cultured on non-coated surfaces, grow in suspension as multicellular aggregates that resemble the neurospheres of cultured neural stem cells.

Complete Growth Medium

The base medium for this cell line is Neural Progenitor Basal Medium, which is supplied as part of the NPMM Neural Progenitor Maintenance Medium Bullet Kit available from Lonza/Clonetics Inc., Catalog No. CC-3209. To make the complete growth medium, add the following components to 500 ml of the base medium:

additives that are supplied with the kit (ATCC does not use gentamycin-amphotericin B)

heat-inactivated fetal bovine serum (FBS) to a final concentration of 10%

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.