The aim of the work was to evaluate cytotoxicity of selected substances using two cellular models representing hepatic and renal tissue. Two groups of xenobiotics were tested - inhibitors of membrane transport (albumin, aprotinin, lysin, maleate, probenecid, bestatin, cytochrome c, ampicilin) and acetylcholinesterase reactivators of oxime type (obidoxime, HI- 6, K027, JM-4, JM-46, K009, K027, K043, K203) and non-oxime type (7-MEOTA, takrin). Two methods were chosen for testing cytotoxicity of reactivators of acetylcholinesterase (RACHE): the first method is based on determination of reduction capacity of cells using tetrazolium dye MTS, the second method is based on measuring activity of proteases in cells. To test cytotoxicity of inhibitors of membrane transport (IMT), a fluorometric method utilizing reduction of resazurin was used. In the case of RACHE two cellular lines were used as a cellular models, porcine renal proximal tubule cells LLC-PK1 and human hepatic cells HEP G2. In the case of IMT native rat isolated renal cells were used together with mentioned cellular lines. The parameter IC50 was determinated for tested substances to evaluate their cytotoxicity. The values IC50 of RACHE determinated by both used methods brought comparable results. The final order of the tested RACHE according...

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Za dodržení všech ustanovení autorského zákona jsou zodpovědné jednotlivé složky Univerzity Karlovy. / Each constituent part of Charles University is responsible for adherence to all provisions of the copyright law.

Upozornění / Notice: Získané informace nemohou být použity k výdělečným účelům nebo vydávány za studijní, vědeckou nebo jinou tvůrčí činnost jiné osoby než autora. / Any retrieved information shall not be used for any commercial purposes or claimed as results of studying, scientific or any other creative activities of any person other than the author.