SpecificityThis assay has high sensitivity and excellent specificity for detection of ARL13B. No significant cross-reactivity or interference between ARL13B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ARL13B and all the analogues, therefore, cross reaction may still exist in some cases.

Product Description specificalIntended Uses: This ARL13B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ARL13B. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||ARL13B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ARL13B antibody and an ARL13B-HRP conjugate. The assay sample and buffer are incubated together with ARL13B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARL13B concentration since ARL13B from samples and ARL13B-HRP conjugate compete for the anti-ARL13B antibody binding site. Since the number of sites is limited, as more sites are occupied by ARL13B from the sample, fewer sites are left to bind ARL13B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARL13B concentration in each sample is interpolated from this standard curve.