This research evaluates the efficacy of cosmetic products manufactured by applying stem cell culture
techniques. The results are obtained after analysing the characteristics of cells cultured in the medium and
evaluating function of cosmetics and the effectiveness using MTS procedure. When culturing human skin
cell and skin stem cell after manufacturing culture medium by mixing approved cosmetics ingredients, the
growth and proliferation of cells were assured. Also associated with skin regeneration from cultured cells
and cell function was remarkably improved. With the culture medium of skin stem cell, Skin essence was
manufactured. This cosmetics showed outstanding improvement effect on skin condition at sensory test of
skin moisturizing, anti-wrinkle, whitening at the time of its use. In addition, a clinical test was conducted to
assess the effectiveness of the Skin essence by applying skin essence containing culture medium after MTS
procedure used as a physical stimulation method in efficient absorption and delivery of cosmetic ingredients.
After MTS procedure, application of skin essence was represented to reduce erythema amount of facial skin
and significantly increase moisture content. We conclude that cosmetics including culture medium ingredients
of skin stem cell promotes the regeneration of skin whitening and anti-winkle and improves the status of trouble
by soothing skin irritation by MTS procedure. Futhermore, this cosmetics showed the striking cosmetic effect of
improving skin condition by maintaining and increasing skin moisture.