IL-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. Accumulating data indicate that p40 secretion precedes that of IL-12 expression. In addition, to its ability to covalently bind to p35 to form IL-12, p40 can bind to p19 to form IL-23, or can form a homodimer designated as IL-12 p80. Elevated levels of IL-12 p80 are correlated with macrophage recruitment and increased inflammation in asthma and respiratory viral infection models. Recombinant human IL-12 p80 is an 80.0 kDa disulfide linked homodimer consisting of two p40 chains of human IL-12.

Catalog #:

003-HIL12P80

Source :

Insect cells

Formulation :

Sterile filtered and then the protein was lyophilized in 1 x PBS pH 6.8.

Stability :

The lyophilized protein is stable for at least 2 years from date of receipt at -200C. Reconstituted IL-12 p80 is stable for at least 3 months when stored in working aliquots with a carrier protein at -200C. Avoid repeated freeze/thaw cycles.

Purity :

Greater than 95% by SDS-PAGE.

Reconstitution :

We recommend a quick spin followed by reconstitution in 200 ul of sterile water for injection for 0.5 mg/ml concentration .
This solution can then be diluted into other aqueous buffers and stored at 40C for 3 months or below -100C for future use.