BUILDING BETTER BREASTS

Stem Cells and Breasts

The breasts are perfect candidates for the use of stem cells for regenerative
therapy. Previously, fat grafts have been used to augment the size and shape of
the breast and have been shown to be effective and safe for women. They fell out
of favor due to a 1987 paper from the American Society of Plastic and
Reconstructive Surgeons which condemned its use due to a perceived decrease in
breast cancer screening efficacy, which was shown to be unfounded [5].
This paper shows that autologous grafts can be and are effective for breast
augmentation and the surgeries performed pave the way for the next generation of
breast augmentation: adipose-derived stem cell therapy coupled with fat grafting
in the breast.

Cell-Assisted Lipotransfer[6]

In cell-assisted lipotransfer, adipose cells are harvested from the patient
and split into two sections. In one of the sections the stem cells are harvested
(adipose-derived stem cells) and the adipose tissue is otherwise discarded. The
isolated stem cells are then added to the other half of the fat mass to create a
tissue with a very high concentration of stem cells within the fatty tissue.
Essentially, the fat tissue itself is used as a scaffold for the stem cells,
enabling them to differentiate into the necessary tissue (fat in this case). The cells
and extracellular matrix provided by the normal fat fraction are the key to allowing
the cells to differentiate into fat cells because these cells provide the optimal microenvironment
(proper scaffolding, matrix density and stiffness, proper supporting cells) to
push the adipose stem cells to differentiate into adipose tissue.
This tissue is then injected into the patient.

Figure 6: Outline of cell-assisted lipotransfer

Figure 7: Injection of stem cells and adipose tissue into breasts

The results of this method are promising. The authors of [6] say that the breasts enlarged using this method
are 2-3 cup sizes larger than before and a lower height but greater natural
contour of the breasts than a comparative increase using an implant. Compared
to patients who underwent standard autologous lipoinjection the patients who
underwent cell-assisted lipotransfer actually saw their breasts increase in size
to a greater degree than those with the conventional therapy because of the
multipotent stem cells contained in the breast tissue.

When compared to standard lipoinjection, the survival rate of the cells in
cell-assisted lipotransfer was much higher. Because of the need for a vasculature
in the injected cellular graft the cells in standard lipoinjection tend to die off and
have a very low survival rate. This is because they do not have a blood supply and essentially starve to death
with no oxygen or nutrients. In cell-assisted lipotransfer the graft is seeded
with a high concentraion of stem cells, allowing it to adapt to its situation.
The stem cells may not only differentiate into fat cells, but into endothelial cells which can then form
a capillary network. This formed network can then supply the injected cells with blood
once it integrates with the host vasculature.[6]

The clinical results from [6] show the safety and efficacy of the method. Currently
no stem cell techniques for breast augmentation are available in the United States
but studies continue. This technique was designed by Cytori Therapeutics and was conducted in Japan.