In article <3sb250$nbu at riscsm.scripps.edu>, "Edward K.L. Chan"
<echan at scripps.edu> wrote:
> Hi netters,
> I wonder if someone may be able to provide a reliable formula to
calculate
>the number of DH5-alpha or XL1-Blue based on O.D. measurement. Thanks in
advance.
>
>
Hello,
Generally, I take an O.D. A600 of 1.0 to equal 1 x 10^9 cfu. If you really
want accuracy I would suggest doing viable counts at O.D. points as the
culture grows.
Good Luck
Brad
===========================================================================
Brad Nicholson |"If it worked the first time, it wouldn't be
Department of Pathology | research."...Brad Nicholson
University of Utah | Live from behind the Zion Curtian.
Salt Lake City, UT 84132 |
brad at corona.med.utah.edu |
or: (801)-581-4365 | My opinions are solely my own.
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