Firstly, the necessities of ecological agriculture development in Big Qinling Eco-city Cluster were discussed. Then, condition endowment of eco-agricultural industrialization in Big Qinling Eco-city Cluster was analyzed from the aspects of basic conditions and differential endowment of eco-agricultural industrialization. Finally, specific forms and functional orientation of eco-agriculture were pointed out Countermeasures for the eco agricultural industrialization in Big Qinling Eco-city Cluster were put forward. Firstly, the government guidance and the media publicity should be strengthened. Secondly, financial support for the eco-agricultural industrialization in Big Qinling Eco-city Cluster should be enhanced. Thirdly, branding strategies of eco-agricultural products in Big Qinling Eco-city Cluster should be implemented as soon as possible.