Trizma® (TRIS base)

TRIS is an established basimetric standard and buffer used in biochemistry and molecular biology1. It is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. TRIS buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification.

El-Harakany, A.A.; Abdel Halima, F.M. and Barakat, A.O. (1984). "Dissociation constants and related thermodynamic quantities of the protonated acid form of tris-(hydroxymethyl)-aminomethane in mixtures of 2-methoxyethanol and water at different temperatures". J. Electroanal. Chem. 162 (1–2): 285–305.