NF-kappa-B inhibitor alpha (also known as IkB alpha) is one of seven members of the IkB class of inhibitor proteins that have been identified to date. NFkBIa inhibits the NFkB complex in unstimulated cells through non-covalent binding. Following stimulation of cells, IkB alpha is phosphorylated so that it dissociates from the NFkB, and then ubiquinated prior to degradation.

Catalog #

N2306-60A

Applications

Suitable for use in ELISA, Immunohistochemistry (frozen), Immunoprecipitation, and Western Blot. Other applications not tested.

Recommended Dilution

Western Blot: 1:500-1:2,000

Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Clone Type

Polyclonal

Isotype

IgG

Host

Rabbit

Source

Human

Concentration

Not determined

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Purity

Serum

Immunogen

NFkBIa peptide corresponding to a region near the C-terminus of the human protein (KLH).