Does CTAB effect 260nm readings?

Happy New Year fellow nettors,
I am trying to obtain conifer DNA using an extraction protocol
which uses CTAB in the extraction buffer. Following extraction a 260 O.D.
reading is otained and a sample is run on a gel along with DNA of know concentration.
The problem is that based on the O.D. readings I am under estimating my actual
conc. when I compare it to the gel. Other people have told me that CTAB does
effect absorbance readings but I find that hard to believe that any CTAB remains
with the DNA after 3 ethanol ppte. are performed. Does anybody have any idea if this is
true and if so how can I get around the problem.
Thanks in advance,
Mark
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Mark Hicks * *
Dept. of Genetics * /*\ <- lodgepole pine
University of Alberta * /***\
Edmonton, Alberta * /*****\ my favorite tree.
CANADA * *
e-mail: mhicks at gpu.srv.ualberta.ca * *
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