Mempro™ Plant-Based VLP Production Using Transient Expression System

VLPs are self-assembled multi-protein structures derived from viral antigens that can simulate native viruses, but are non-infetious due to lacking the viral genetic materials. Based on this advantage in non-infectiousness, VLP can be applied for vaccine development. It is reported that intact membrane proteins with native conformation can be integrated in to VLPs (this complex is termed lipoparticles). Thus, Lipoparticles are widely used as a powerful tool for integral membrane protein poduction. Self-assembled VLPs capture desired membrane proteins and bud them off from the cell membrane in the direct manner. In additon to membrane protein production, Creative Biostructure can offer custom Mempro™ VLP production services for various biological studies, including antibody screening, phage and yeast display, vaccines production, ligand binding assay and small molecules delivery.

Creative Biostructure can provide plant-derived VLP production using transient expression system. With this expression system, a soil bacterium, Agrobacterium tumefaciens,is employed to infect the plant cells, along with the transfer of viral genetic materials in the form of a mobile DNA fragment are also into the nucleus of the infected plant cell. Owing to this mobile DNA fragment is not integrated into the plant genome, the plant cells are just transiently transformed. The performance of transient expression can be divided into several procedures, including: