Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

adenocarcinoma

Age

63 years old adult

Gender

male

Ethnicity

Black

Applications

Cells from this cell line produce tumors in nude mice when injected either subcutaneously or orthotopically (intraprostatic).

This cell line is suitable for studying cell growth regulation by androgens.

Storage Conditions

liquid nitrogen vapor phase

Derivation

MDA PCa 2b was established from a bone metastasis of 63 year old Black male with androgen-independent adenocarcinoma of the prostate.

Clinical Data

male

­

63 years old adult

Black

Antigen Expression

prostate specific antigen (PSA)

Receptor Expression

androgen receptor, expressed

Tumorigenic

Yes

Effects

Yes, forms tumors in nude mice

Comments

The cell line expresses prostate specific antigen (PSA) and androgen receptor, grows in vitro and in vivo, and is androgen sensitive.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 20%

25 ng/ml cholera toxin

10 ng/ml mouse Epidermal Growth Factor

0.005 mM phosphoethanolamine

100 pg/ml hydrocortisone

45 nM selenious acid

0.005 mg/ml bovine insulin

Do not filter complete medium.

Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.