製品の概要

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information
This antibody only detects the active (cleaved) form of Caspase-3 and does not recognize the pro form of Caspase-3.
PubMedID 19789217 describes the detection of human cells injected into mice.

アプリケーション

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション

Abreviews

特記事項

IHC-Fr

Use at an assay dependent concentration.

WB

1/500. Detects a band of approximately 17 kDa (predicted molecular weight: 32 kDa).

ICC/IF

1/100 - 1/250.

追加情報

Is unsuitable for Flow Cyt or IP.

ターゲット情報

機能

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp--Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

組織特異性

Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

配列類似性

Belongs to the peptidase C14A family.

翻訳後修飾

Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.

I would not recommend using an autoclave, as this could damage the tissue. I would suggest using a pressure cooker or a microwave. The time and temperature may require some optimization. For the time, I can suggest...

Pro-caspase 3 is cleaved between amino acids 175 and 176 of the sequence described in the UniProt entry, P42574. http://www.uniprot.org/uniprot/P42574Antibody ab32042 is raised against the N terminus of subunit p17.