Using a functional monomer(methyl methacrylate, acrylamide, ethyl acrylate or acrylic acid) and a crosslinker(TMPTA), molecularly imprinted polymers(MIPs) with recognition properties to dibenzoyl-L-tartaric acid(L-DBTA) were prepared by photopo-polymerization method in the presence of a template(L-DBTA) in acetonitrile solution.The experimental results indicated that the MIPs had higher L-DBTA binding ability than D-DBTA′s and also higher than corresponding non-imprinted polymers. The binding ability of MIPs is the highest at template (n_(L-DBTA)/n_(AA)/n_(TMPTA)= 1/4/12 as photo-polymerization time was 1.5h and photopolymering in a ice-water bath under a 100W ultraviolet lamp about 0.07m. Based on the optimal experimental results, it was found that the highest separation factor (α) of L-DBTA/D-DBTA of the MIPs could reach to be 5.41.