Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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CRISPR genome editing

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Are lyophilized / dried oligos stable at room temperature?

For room temperature or 4°C storage, oligos that are resuspended in TE buffer (10 mM Tris pH 8.0, 0.1 mM EDTA; such as IDTE), are more stable than dry oligos. Oligos that are stored in water are the least stable. However, at 4°C, oligos stored under all of these conditions are stable for at least 60 weeks. Also, it is ideal to store oligos in the dark. Exposure to UV light should be avoided, and ambient lab light should be minimized, particularly for some types of modified oligos. Please see the Technical Report, Oligonucleotide Stability Study, for data on oligonucleotide storage and a more thorough explanation.