Electrospinning is a promising method to fabricate biomedical products for a variety of applications ranging from wound dressings and controlled drug release systems to scaffolds for tissue engineering. The fabrication of fleeces with fibers of defined diameters instead of materials containing undesired beads often presents a challenge when spinning biomolecular fibers. An interesting solution for cell culture applications is the spinning of polymers like polyamide and polystyrene and processes them afterwards via pico-second UV-laser radiation.

The study comprised the investigation of the morphology of electrospun nanofibers produced by means of a conventional experimental electrospinning device to examine the formation of fleeces made of different materials and further processed by different laser radiation patterns. Different patterns of ablated areas of the fleeces are compared in cell cultures.

Processed fleeces were tested in cell culture with different cell types. The fiber shape, diameter and mesh size were determined by SEM investigation of the non-woven without further preparation. Cells were tested on viability via MTT assay and their morphologies were studied by confocal laser scanning microscopy.