Properties

Form

Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.

Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

WB

Use a concentration of 0.1 - 0.2 µg/ml.

ELISA

Use a concentration of 0.5 µg/ml.

Neutralising

Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of hRANTES (100.00 ng/ml), a concentration of 5.0 - 7.0 µg/ml of this antibody is required.

IHC-P

Use a concentration of 2 µg/ml.

Target

Function

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.

Tissue specificity

T-cell and macrophage specific.

Sequence similarities

Belongs to the intercrine beta (chemokine CC) family.

Post-translationalmodifications

N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for RANTES using ab9679 at 1/150 dilution in immunohistochemical analysis. Goat anti-Rabbit HRP was used as the secondary antibody. Heat mediated antigen retrieval using EDTA at pH 9 was used before commencing with the IHC protocol.

Formalin-fixed, paraffin-embedded mouse colon tissue stained for RANTES using ab9679 at 1/150 dilution in immunohistochemical analysis. Goat anti-Rabbit HRP was used as the secondary antibody. Heat mediated antigen retrieval using EDTA at pH 9 was used before commencing with the IHC protocol.

The best choice from what we have is the goat polyclonal ab10590. Other antibodies we have that are raised against RANTES may also work for immunocytochemistry but they have not been tested in this application.

Thank you for contacting us. I have been in contact with the lab, I can confirm that ab9679 has already been reconstituted in 200ul sterile water. Since this antibody was lyophilised in PBS, this means that upon the addition of the water, the anti...

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