Norgen’s Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format) provides an efficient method for the purification of fragmented free-circulating DNA from 0.4 mL to 2 mL of human plasma or serum. The kit is able to isolate all sizes of circulating DNA. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating DNA from large sample volumes. DNA can be isolated from either fresh or frozen samples using this kit.

Typical yields of purified free-circulating DNA will vary depending on the input sample (1-100 ng/mL circulating DNA in human plasma), with more concentrated samples tending to yield more free-circulating nucleic acids. Preparation time for a single sample is less than 45 minutes. The purified plasma/serum free-circulating DNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis.

Kit Specifications

Minimum Plasma/Serum Input

400 μL

Maximum Plasma/Serum Input

2 mL

Minimum Elution Volume

100 μL

Time to Complete Purifications

< 45 minutes

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15-25°C) for up to 1 year without showing any reduction in performance.