Citation and License

Diagnostic Pathology 2012, 7:159
doi:10.1186/1746-1596-7-159

Published: 22 November 2012

Abstract

Objective

MicroRNA-100 (miR-100) has been demonstrated to be downregulated in bladder cancer
tissues, and enforced expression of this miRNA may inhibit cell growth and colony
formation of human bladder cancer 5637 cells in vitro. However, the clinical significance
of miR-100 in human bladder cancer has not yet been elucidated. Thus, the aim of this
study was to investigate the diagnostic and prognostic values of miR-100 in this disease.

Methods

Expression levels of miR-100 in 126 pairs of bladder cancer and adjacent normal tissues
were detected by TaqMan real-time quantitative RT-PCR assay. In order to determine
its prognostic value, overall survival (OS) and progression-free survival (PFS) were
evaluated using the Kaplan-Meier method, and multivariate analysis was performed using
the Cox proportional hazard analysis.

Conclusion

Our data offer the convincing evidence that miR-100 may play an important role in
the progression of bladder cancer and that the reduced expression of this miRNA may
be independently associated with shorter PFS and OS of patients, suggesting that miR-100
might be a potential marker for further risk stratification in the treatment of this
cancer.