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The Binding Site Optilite is an automated, bench-top, random-access turbidimeter (Figure 38.2). IgAκ, IgAλ, IgMκ and IgMλ Hevylite assays are currently available for use on the instrument, and IgGκ and IgGλ assays are in development.

The Hevylite assay times are 11 minutes for IgMκ and IgMλ assays and 18 minutes for IgAκ and IgAλ. Calibration curves are generated using a single calibrator fluid which is diluted by the instrument, and curves are validated using the two control fluids supplied with the kits.

If a Hevylite result is outside the initial measuring range at the standard (1+9) dilution, the sample is automatically re-diluted by the instrument. Assay parameters define two higher and one lower automatic re-dilution; very high level samples may require further offline dilution.

Hevylite assays have good within-run and between-run precision [891][892][893][894]. Performance characteristics are summarised in Table 38.2. Antigen excess (prozone) parameters exist for IgM Hevylite assays. Samples detected as being in antigen excess are automatically flagged by the instrument with a “prozone detected” flag and the sample is automatically re-assayed at the higher dilution.