Application Information: NEK2 antibody

Recommended Starting Dilutions:For WB: Use at 0.5 μg/mlOptimal dilutions should be determined experimentally by the researcher.

Form Supplied

Liquid

Concentration

0.2
mg/ml
(Please refer to the vial label for the specific concentration)

Purification

Affinity purified

Specifications: NEK2 antibody

Full Name

NIMA (never in mitosis gene a)-related kinase 2

Product Description

Sheep Polyclonal antibody to NEK2

Background

Protein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore. Phosphorylates SGOL1. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.

Synonyms

HsPK 21, HsPK21, NEK2A, NLK1

Cellular Localization

Co-localizes with SGOL1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope. Has a nucleolar targeting/ retention activity via a coiled-coil domain at t