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Protocols - Immunofluorescence: Rabbit Anti-Murine RELMβ

The following protocol used B6 mice that were infected with 200 embryonated Trichuris muris eggs and sacrificed 21 days post-infection. The tip of the cecum was removed, rinsed in PBS, and fixed in 4% PFA. Following fixation, the tissue was embedded in paraffin and cut into 5 μM sections.