The Cystatin C test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Cystatin C in human biological samples.﻿ In this assay the CystatinC present in samples reacts with the anti-Cystatin C antibodies which have been adsorbed to the surface f polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Cystatin Cantibodies conjugated with horseradish peroxidase (HRP), are added.﻿