Western blot analysis is one of the most common methods used in the lab today, because of its versatility in detecting and measuring specific target proteins in a sample and revealing their activity and any post-translational modifications. Western blotting is a multi-step process that can take 1-2 days to complete, depending on the methods used. Below are videos that detail the common steps to perform a Western blot, from separating proteins in a lysate to developing the blot.