FAQ: How long should I incubate cells on ice after DNA has been added (NEB #C3013H and NEB #C3013I)?

Incubating DNA with T7 Express lysY/Iq competent cells on ice for 30 minutes is recommended. When tested with pUC19, an incubation time of just 2 minutes did not significantly lower the transformation efficiency (see Figure on the main product page).