Haha.
I found the appropriate equation and a way to solve in Kaleidagraph.
F = (Fmax.[L])/([L]+Kd) where F = change in fluorescence and L is ligand.
Plotting this in Graphing Calculator (finally found a use for it!) gives
the 'correct' shape curve, and putting the follwowing formula into
Kaleidagraph yields Kd: y=(m1*m0)/(m0+m2) where m1 = F and m0=[L].
--
Richard P. Grant 0x5F9559B1 MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
-- 'Let the beetles have their say' --