DSpace Collection:http://hdl.handle.net/10204/893
Mon, 05 Dec 2016 00:15:07 GMT2016-12-05T00:15:07ZDevelopment of a novel rDNA based plasmid for enhanced cell surface display on Yarrowia lipolyticahttp://hdl.handle.net/10204/6709
Title: Development of a novel rDNA based plasmid for enhanced cell surface display on Yarrowia lipolytica
Authors: Bulani, S; Moleleki, L; Albertyn, J; Moleleki, J
Abstract: In this study, a novel rDNA based plasmid was developed for display of heterologous proteins on the cell surface of Yarrowia lipolytica using the C- terminal end of the glycosylphosphatidylinositol (GPI) anchored Y. lipolytica cell wall protein 1 (YlCWP1). mCherry was used as a model protein to assess the efficiency of the constructed plasmid. Y. lipolytica transformants harbouring the expression cassettes showed a purple colour phenotype on selective YNB-casamino plates as compared to control cells indicating that mCherry was displayed on the cells. Expression of mCherry on cells of Y. lipolytica was confirmed by both fluorescent microscopy and flow cytometry. Furthermore, SDS-PAGE analysis and matrix-assisted laser desorption/ionization (MALDI)-time-of (TOF)-mass spectrometry (MS) peptide mass fingerprinting (PMF) confirmed that the protein cleaved from the yeast cells using enterokinase was mCherry. Efficient cleavage of mCherry reported in this work offers an alternative purification method for displayed heterologous proteins on Y. lipolytica cells using the plasmid constructed in this study. The developed displaying system offers great potential for industrial production and purification of heterologous proteins at low cost.
Description: Copyright: 2012 BioMed Central. This is an Open Access journal. This journal authorizes the publication of the information herewith contained. Published in AMB Express, vol. 2(27), pp 1-8Tue, 01 May 2012 00:00:00 GMThttp://hdl.handle.net/10204/67092012-05-01T00:00:00ZPALM and STORM: unlocking live-cell super-resolutionhttp://hdl.handle.net/10204/5414
Title: PALM and STORM: unlocking live-cell super-resolution
Authors: Henriques, R; Griffiths, C; Rego, EH; Mhlanga, MM
Abstract: Live-cell fluorescence light microscopy has emerged as an important tool in the study of cellular biology. The development of fluorescent markers in parallel with super-resolution imaging systems has pushed light microscopy into the realm of molecular visualization at the nanometer scale. Resolutions previously only attained with electron microscopes are now within the grasp of light microscopes. However, until recently, live-cell imaging approaches have eluded super-resolution microscopy, hampering it from reaching its full potential for revealing the dynamic interactions in biology occurring at the single molecule level. Here we examine recent advances in the super-resolution imaging of living cells by reviewing recent breakthroughs in single molecule localization microscopy methods such as PALM and STORM to achieve this important goal.
Description: Copyright: 2011 Wiley Blackwell. This is an ABSTRACT ONLYSun, 01 May 2011 00:00:00 GMThttp://hdl.handle.net/10204/54142011-05-01T00:00:00ZEffect of expressing an anti-HIV lectin, Griffithsin, in different plant cellular compartmentshttp://hdl.handle.net/10204/4457
Title: Effect of expressing an anti-HIV lectin, Griffithsin, in different plant cellular compartments
Authors: Stark, T; Chakauya, E; Kabamba, A; Morris, L; Rybicki, E; Chikwamba, R
Abstract: Sub-Saharan Africa remains the region most heavily affected by HIV, and the majority of new infections from heterosexual contact are in women. Griffithsin (GRFT), a lectin isolated from the red algae Griffithsia, has shown extreme potency against the Human Immunodeficiency Virus (HIV) in vitro in very low quantities. The molecular target of GRFT is the high mannose residues on the viral gp120 coat protein. Low cost production of GRFT and other anti-HIV drugs at minimal costs is crucial seeing that the high demand for anti-HIV drugs reside in poor developing countries.
Description: CSIR 3rd Biennial Conference. Science Real and Relevant, CSIR International Convention Centre, Pretoria, 30 August – 1 September 2010Sun, 01 Aug 2010 00:00:00 GMThttp://hdl.handle.net/10204/44572010-08-01T00:00:00ZMorphological variation of sorghum landraces from semi-arid areas of Zimbabwehttp://hdl.handle.net/10204/4169
Title: Morphological variation of sorghum landraces from semi-arid areas of Zimbabwe
Authors: Mujaju, C; Chakauya, E
Abstract: Sorghum (Sorghum bicolor) is one of the most important cereal crops cultivated in, the semi-arid areas of Zimbabwe where persistent drought, high temperatures and poor edaphic conditions are endemic. Because of their adaptation, landraces are the mainstay of smallholder agriculture in such areas. Unfortunately, limited studies have been done to quantify the genetic diversity of this material in order to formulate the necessary conservation strategies. This study analysed the genetic diversity of 47 sorghum landraces from two districts of Zimbabwe, Nyanga North and Tsholotsho using 24 Sorghum agromorphological descriptors. Genetic similarities (Manhattan coefficient) were calculated and genetic relationships between accessions were analysed by principal component analysis and cluster analysis. The landraces grouped into six clusters according to the geographical location where they were collected from, suggesting environmental adaptation. Accessions with the same name had a tendency to group together, although some material was found scattered throughout the dendrogram. Agromorphological traits were highly variable even for landraces with the same farmer-given name and source. The results also alluded to the heterogeneity of farmer varieties, whose naming appears to be a function of a few traits. The study suggests that optimisation of on-farm conservation strategy for this germplasm should primarily focus on high diversity areas and perhaps recognise those traits envisaged to be of importance by farmers for varietal identification
Description: Copyright: 2008 Asian Network for Scientific InformationTue, 01 Jan 2008 00:00:00 GMThttp://hdl.handle.net/10204/41692008-01-01T00:00:00ZElicitor and fusarium-induced expression of NPR-1 like genes in bananahttp://hdl.handle.net/10204/3378
Title: Elicitor and fusarium-induced expression of NPR-1 like genes in banana
Authors: Endah, R; Beyene, G; Kiggundu, A; Van den Berg, N; Schlüter, U; Kunert, K; Chikwamba, RK
Abstract: NPR1 is an essential positive regulator of salicylic acid-induced PR gene expression and systemic acquired resistance. Two novel full-length NPR1-like genes; MNPR1A and MNPR1B, were isolated by application of the PCR and RACE techniques. The two identified MNPR1-sequences differed greatly in their expression profile using qRT-PCR following either elicitor or Foc treatment. MNPR1A was greatly expressed after Foc treatment with higher and earlier expression in the Foc-tolerant cultivar GCTCV-218 than in the sensitive cultivar Grand Naine. In comparison, MNPR1B was highly responsive to SA, but not to MeJA treatment, in both the tolerant banana cultivar GCTCV-218 and the more sensitive cultivar Grand Naine. Expression of the MNPR1 genes further directly related to PR gene expression known to be involved in fungal resistance. Reduced sensitivity to Foc in GCTCV-218 might be partially attributed to the higher and an earlier expression of both MNPR1A and PR-1 in this cultivar after Foc treatmen
Description: Author Posting. Copyright Elsevier, 2008. This is the author's version of the work. It is posted here by permission of Elsevier for personal use, not for redistribution.Sat, 01 Nov 2008 00:00:00 GMThttp://hdl.handle.net/10204/33782008-11-01T00:00:00ZIssues on global food securityhttp://hdl.handle.net/10204/3377
Title: Issues on global food security
Authors: Chikwamba, RK
Abstract: The presentation outlines the global status of food security and implications for meeting the Millennium Development Goals targets, with particular focus on Sub-Saharan Africa; trends in hunger, food availability, food distribution, consumption; underlying causes of food insecurity; and the role of technology in addressing food security
Description: Bio2Biz Conference, 29 Septemner 2006Fri, 29 Sep 2006 00:00:00 GMThttp://hdl.handle.net/10204/33772006-09-29T00:00:00Z