The goal of the PneumocystisExpressed
Sequence Tag portion of the project is to provide a rapid gene
inventory of genes expressed in Pneumocystis.

Two libraries have been analyzed to initiate the PneumocystisExpressed Sequence Tag portion of the project: a
well-known library produced by Jeff Edman and a recent library constructed by
George Smulian. The Edman library contains multiple short inserts and
significant host contamination that prevented its use. The Smulian library
appears to contain long inserts and host contamination of about 15%, an
acceptable level for this purpose. We have entered a production phase on
the Smulian library. We anticipate adding 500 clones per week for the next
several weeks and plan to normalize the cDNA library when approximately
2500-5000 clones have been processed.

Data analysis is proceeding on several fronts.
Processing the EST data, access
to the data, and initial blast searches has been spearheaded by
Michael Weise at Georgia. Initial efforts to categorize and review the
ESTs in terms of potential functions has been initiated by Chuck Staben (view in
HTML or in Excel
format).

We have not yet arranged access to the clones
themselves. At this point, should you wish to receive an individual clone
or make further inquiries about the libraries, please contact Chuck
Staben.