Abstract:
Low birth weight (LBW) is the most common cause of perinatal mortality, causing almost 30 percent of neonatal deaths. On the other hand, maternal short stature is known to cause a lot of obstetric complications like cephalopelvic disproportion and arrest of labor, intrauterine asphyxia, intrauterine growth retardation. The objective of our study was to find out whether there was any significant statistical association between maternal height and the birth weight of the neonate. We identified a group of low birth weight neonates (n=54) and a control group (n=51) of normal weight neonates at term in Mediciti Hospital over a period of 1 year and retrospectively looked the maternal heights for both groups. Inclusion criteria being mothers who delivered at term, mothers who had a hemoglobin level more than 10 gm/dl, mothers with relatively uneventful antenatal without any significant obstetric or medical complications during the pregnancy, and neonates with relatively uneventful post-natal periods without any significant pediatric or medical complications. The odds of having been born of a mother of short stature are more than three times greater for a low birth weight baby than a normal weight baby. The mean of birth weights of babies born to mothers of normal height is more than the mean of birth weights of babies born to mothers of short stature by 277.01 gm. This study reaffirms the observation that maternal height has a direct effect on the weight of the newborn and we propose that maternal short stature be identified as an independent risk factor for low birth weight.

Abstract:
Fly-ash was evaluated for possible use as carrier for Azotobacter and Azospirillum formulation. Azotobacter and Azospirillum strains were isolated from healthy wheat rhizosphere soil and bio-formulated in fly-ash (300 meshes). Fly-ash based Azotobacter and Azospirillum formulation alone and in combination with chemical fertilizer was evaluated for bio-efficacy on wheat. Population of Azotobacter and Azospirillum was also evaluated in treated soil. The results of the studies showed that, seed treatment with Azotobacter and Azospirillum and soil treated with chemical fertilizer alone and in combination significantly enhanced the seed germination, plant height, plant biomass and crop yield compared to control. Chemical fertilizer treated wheat plant observed more effective bio-efficacy than bio-fertilizers treated wheat but reduced (destroyed) the microbial population in soil. Whereas Azotobacter and Azospirillum treated soil observed significantly enhanced the microbial population with slightly lesser plant growth as compared to chemical fertilizer. In the present study it was showed that utilization of fly-ash as carrier in bio-fertilizer formulations emerged as safe and effective alternatives. Use of fly-ash as carrier in these formulations is an effective way of utilization of problematic fly-ash waste in a useful manner. International Journal of Engineering, Science and Technology, Vol. 2, No. 7, 2010, pp. 31-35

Abstract:
Bacillus represents microbes of high economic, medical and biodefense importance. Bacillus strain identification based on 16S rRNA sequence analyses is invariably limited to species level. Secondly, certain discrepancies exist in the segregation of Bacillus subtilis strains. In the RDP/NCBI databases, out of a total of 2611 individual 16S rDNA sequences belonging to the 175 different species of the genus Bacillus, only 1586 have been identified up to species level. 16S rRNA sequences of Bacillus anthracis (153 strains), B. cereus (211 strains), B. thuringiensis (108 strains), B. subtilis (271 strains), B. licheniformis (131 strains), B. pumilus (83 strains), B. megaterium (47 strains), B. sphaericus (42 strains), B. clausii (39 strains) and B. halodurans (36 strains) were considered for generating species-specific framework and probes as tools for their rapid identification. Phylogenetic segregation of 1121, 16S rDNA sequences of 10 different Bacillus species in to 89 clusters enabled us to develop a phylogenetic frame work of 34 representative sequences. Using this phylogenetic framework, 305 out of 1025, 16S rDNA sequences presently classified as Bacillus sp. could be identified up to species level. This identification was supported by 20 to 30 nucleotides long signature sequences and in silico restriction enzyme analysis specific to the 10 Bacillus species. This integrated approach resulted in identifying around 30% of Bacillus sp. up to species level and revealed that B. subtilis strains can be segregated into two phylogenetically distinct groups, such that one of them may be renamed.

Abstract:
A bacterial strain, designated AcBz01, was isolated from a water sample collected from Gomti River, Lucknow, India, and identified using a molecular approach. On the basis of the bacterial 16S rRNA gene sequence phylogeny and comparison of this gene sequence with sequences in Ribosomal Database project II, evidence given in this study, it is proposed that isolate is closely related to members of the genus Acinetobacter. Identification and annotation of regulatory elements in the 16S rRNA gene and characterization of their interaction with the respective transcription factor can provide basis for better understanding of the mechanism of network of gene interaction of functionally related genes. The identification of such sites is relevant for locating promoter boundary of a gene and it also allows the prediction of specific gene expression pattern and response to disturbances in a known signaling pathway. Computational identification of regulatory elements and Transcription Factor with their binding sites in 16S rRNA gene of Acinetobacter sp. was performed using BPROM tool. We predicted the regulatory elements are TSS, -10 box, -35 box and three Transcription Factor (narP, ompR and fadR) with their binding sites in the upstream region of 16S rRNA gene of Acinetobacter sp. AcBz01. The GenBank accession number for 16S rRNA gene of Acinetobacter sp. AcBz01 is EU931637.

Abstract:
The clinical manifestations of honeybee sting are myriad. Although most such stings are followed by local allergic reactions only but it can be potentially serious. The rare manifestations comprise of encephalitis, acute disseminated encephalomyelitis, parkinsonism, polyneuritis, Guillain-Barre syndrome, myocardial infarction, pulmonary oedema, bleeding manifestations, haemolytic anaemia and renal failure. The literature regarding honeybee sting from India is sparse. We report a rare case of honeybee sting induced extra pyramidal manifestations in form of akinetic rigid parkinsonism which developed 48 hours after multiple honeybee stings.

Abstract:
Due to the lack of representation of an object-oriented database on hand-held devices, the present work describes an approach to send the information in the form of an object-oriented database on hand-held devices. A smart phone is selected as a hand-held device and connectivity between the object-oriented databases has been established by the use of the object-oriented programming language C#.Net. For modeling purpose, Unified Modeling Language (UML) is used to specify a UML Class Model and Sequence diagram. A concept of star schema is also used to represent the object-oriented database.

Abstract:
Nowadays, the size of database of any business organization is increasing and many of the companies are shifted the old structured database into the object oriented database. Due to increase of size of database complexity of database is increasing therefore, it is necessary to optimize the object oriented query response time from the complex object oriented database. In the present paper, a real case study of Life Insurance Corporation of India is taken and sample object oriented database is designed by the use of SQL Server 2008. A UML model is designed for computing the object oriented query response time. Table and graph are also represented for the computed records in five runs.

Abstract:
Impedance mismatch of data flow is the major problem in the relational database when one is using the web based application development on the computer system. It effects the development of scalable and reusable web application. Due to this, the research cost of development and maintenance increases. Therefore the present work is an attempt towards an object-oriented database system by using Db4o to overcome the impedance mismatch problem. In the current work, the development of web application for Indian Postal Services by using Db4o is demonstrated on dot NET platform. Before developing the application, the authors used a Unified Modeling Language (UML) model in the form of UML class, sequence and use-case diagrams. The Db4o is used to store the object-oriented database and the performance of Db4o is observed through several object-oriented queries and corresponding results are demonstrated.