Abstract: Appropriate treatment and wound care accelerate the healing process and prevent infection. The aim of present study was to evaluate the effect of HESA-A (a drug of marine-plant origin) on the wound healing process. The effect of HESA-A at concentrations of 2.5% (mixture of 2.5% drug and 97.5% chow), 5 and 10% on the healing process of a 35 mm long full-thickness wound was prepared dorsally on rats, evaluated through measuring the length of the healed region on different days and conducted tensiometery experiments after complete wound healing. The mean percentage of wound healing on days 12, 14, 16 and 18 in control group changed in the group treated with 2.5% HESA-A from 62.5, 71.1, 86.7 and 100-75.5, 91.2, 100 and 100 (p<0.001), respectively; in the group treated with 5% HESA-A to 88.5, 98.6, 100 and 100 (p<0.001), respectively and in the group treated with 10% HESA-A to 92.8, 100, 100 and 100 (p<0.001), respectively. The stress changed from 16.5±0.9 Newton (N) in the control group to 19.2±1.5 N, 24.2±1.3 N (p<0.001) and 32.1±0.7 N (p<0.001) in the groups treated with 2.5, 5 and 10% HESA-A, respectively. The strain increased from 14.8±0.6 millimeter (mm) in the control group to 16.5±0.8, 25.7±0.4 (p<0.001) and 35.7±1.1 mm (p<0.001) in the groups treated with 2.5, 5 and 10% HESA-A, respectively. Our findings suggest, that HESA-A may have accelerated the skin wound healing process in rat in a dose-dependent manner and it seems increased tissue strength through stimulating collagen formation.