MAINTENANCE OUTAGE: The University Wiki Service will undergo maintenance Tuesday evening, from 6:00 pm to 8:00 pm. During this 2 hour time period https://wikis.utexas.edu may be unavailable. Users are advised to save content locally that may be needed during this time and to otherwise save all edits as unsaved work may be lost.

Ready to submit samples? Use these links

To submit a new project or get detailed pricing information, use these links. You'll be given a 6-digit request ID to refer to your request later. Your requests stay here until you're ready to submit samples. You return to this link when you're ready to submit your samples.

1. Log in in the upper right corner with your EID

Don't have an EID, or haven't submitted a sample to us before? Please go read this page for a short orientation before you get started.

Latest news

24 Nov 14

To contact the GSAF about a sequencing project, please use the new email alias "gsaf <at> utgsaf.org".Emails sent to this address take priority over emails sent directly to the Director or Lab Manager.

Scott Hunicke-Smith presented GSAF updates at the Byte Club meeting. The presentation covers new user-accessible equipment, the NextSeq 500 and HiSeq V4 upgrade, pricing changes, new library prep methods, and new data storage options. Here is a PDF copy of the presentation.

The GSAF is proud to announce several major additions and changes to our service offerings. All are effective immediately, and the costs listed below are for internal UT researchers. We have integrated these new NGS prices into our LIMS job/sample submission system so you will have an accurate price for any platform and prep before you submit your samples.

Genotyping-by-sequencing for the standard price of $25/sample including sequencing (plus $200 for projects of less than 176 samples).

Lower library prep prices - Thanks to well-validated automation, DNA fragment libraries are now $60/sample (>=8 samples) and directional RNA libraries including poly-A mRNA enrichment are now $130/sample (>= 8 samples). In addition to being less expensive, we have also found fewer sequencing issues with libraries prepared at the GSAF and want to pass that savings on to you.

Lower sequencing prices - our efficiency has improved thanks to hard work in process optimization and automation and we can pass that on to you. The new MiSeq and HiSeq instruments are now functioning smoothly and our queue times have been reduced substantially since this past summer. Both MiSeq instruments are capable of the higher output (20-25 million reads) 2x300 bp Version 3 sequencing chemistry, and both HiSeq 2500's are capable of Rapid (up to 2x150 bp) and High Output ("standard" - up to 2x100 bp) runs.

As always, please let us know if you have any questions, concerns, or comments.

Sincerely,

Scott, Jessica, and all the GSAF staff and Randy Hughes and all the ARL staff.

Getting started

Read this page further to learn what instruments we have and what we're experienced with.

Attend UT's Next-gen sequencing club meetings on the first and third Thursdays of the first month of each semester, 3:00 pm., MBB 2.204 - a great place to start learning about NGS techniques applied to real projects. Sign-up on our mailing list by joining "gsafusers" at UT's list server - just select "subscribe" and enter "gsafusers".

Protocols, instruments, computers, and software of the UT GSAF

The GSAF serves a wide array of customers and has gained considerable experience in preparing samples and analyzing data for NGS projects. We have industry-leading platforms and some user-accessible instruments. If you're just considering a project or are ready to sequence, we're here to give you great data quickly and affordably. Click to expand...

The GSAF is housed in approximately 2,000 square feet of controlled-access laboratory space and is an experienced NGS facility. Launched in 2008, the GSAF currently processes over 5,000 NGS samples per year.

Our equipment and capabilities include:

Two Illumina HiSeq 2500 next-generation DNA sequencers and associated peripherals. The HiSeq can generate over 600 gigabases of sequence in an 11-day run or 120 gigabases in a ~1 day rapid run. The GSAF has experience generating small RNA, mRNA, genomic DNA fragment, RAD (including ddRAD), bacterial and fungal metagenomics, and genomic DNA large-insert mate-pair libraries for the Illumina platform.

Two Illumina MiSeq next-generation DNA sequencers and associated peripherals. The MiSeq is intended for lower data output, faster turn-around time projects, or for projects requiring longer read lengths (up to 600 bp per template, as two 300 bp sequences).

Welcome to the UT GSAF Wiki - a central source of information for next-gen sequencing at UT Austin.

All are welcome to explore. With a UT EID you may also edit and contribute. Please sign up for our user group email to get notifications of upcoming seminars, NGS club meetings, and changes to service. Just hit "subscribe", enter your email address, and "submit". You don't have to be at UT to subscribe.