A carp myosin heavy chain gene isoform was isolated from a genomic clone, restriction mapped and partially sequenced to reveal the location of various exons. The clone
contains a complete gene of approximately 12.0 kb which is half the size of the corresponding mammalian and avian myosin heavy chain genes. The mRNA transcript of this gene, however, is the same size as mammalian and avian striated muscle myosin heavy chain genes (about 6000 nucleotides), illustrating that the difference in size at the genomic level is due to shorter introns. A 169 bp NsiI restriction fragment containing only the 39 untranslated
region of this gene was subcloned and used as an isoform-specific probe to study the expression of this particular isoform. Hybridisation analysis could only detect
expression of this myosin heavy chain gene in the white muscle of adult carp that had been subjected to an increased environmental temperature. No expression of
this gene was detected in carp under 1 year of age. In situ hybridisation demonstrated that expression of this gene is
limited to small-diameter white muscle fibres of adult carp, which are thought to be responsible for muscle growth by
fibre hyperplasia.