Excessive inhalation of vapor may cause symptoms that parallel ingestion, ranging from headache to unconsciousness, depending upon the duration and level of the exposure. May cause headache, dizziness, nausea, vomiting, abdominal pain, and unconsciousness.

Notes:

Make sure the H2O is sterile.

Make sure everything you use in the experiment is sterile, including yourself (spray everything down with 70% ethanol).

Formamide is best accessible when taking 40 ul and putting it in a separate tube, apart from the bottle.

Make sure to use a different tip each time you grab water, yeast, or formamide.

Return bottle of formamide to frig when done.

Procedure:

Grab the bottle of neat formamide and pipet out 36 ul into a PCR Tube.