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Heat-stable Alpha-Amylase HT-800

A low pH tolerant Alpha-Amylase with robust activity and super thermal stability for the production of starch sugar.

Product Specifications

DESCRIPTION

HT-800 is a low pH tolerated Heat-stable Alpha-Amylase
produced from selected strains of Bacillus
licheniformis through submerged fermentation and refining extraction
processes. HT-800 is an endoamylase which can rapidly decrease the viscosity of
gelatinous starch solutions by randomly hydrolyzing starch, glycogen and its degradation
products within the alpha-D-1,4 glucosidic bonds to produce soluble dextrins
and oligosaccharides.

As a robust Alpha-Amylase with superior
thermal stability, HT-800 has found
its wide application in the production of starch sugar.

PRODUCT CHARACTERISTICS

Declared Enzyme: Alpha-Amylase

Systematic Name:EC 3.2.1.1, 1,4-α-D-glucan glucanohydrolase

Activity: 80,000 U/ml (Minimum)

Appearance: Sepia brown Liquid

Product pH:5.0 to 7.0

Specific gravity: ≤1.25 g/ml

EFFECT OF PH

HT-800 can
work at pH ranging from 5.0 to 7.0 with its optimal pH range from 5.4 to 6.0. The
exact pH optimum depends on the actual processing conditions, including
temperature, substrate concentration and reaction time.

EFFECT OF TEMPERATURE

For
interval liquefaction process, the optimum temperature of HT-800 is above 90°C.
It liquefies starch substrates promptly at 95 to 97°C and maintains highly active at 100°C. For consecutive liquefaction process, HT-800
demonstrates exceptional thermal stability at temperatures from 105 to 110°C and
keeps on liquefying efficiently for 5 to 7 minutes.

CONCENTRATION OF CALCIUM

The presence of calcium increases the thermal stability of HT-800. This enzyme requires a very low calcium concentration of 20 to 50 ppm to ensure its enzyme stability.

INHIBITORS

Copper, titanium, and cobalt ions are
moderate inhibitors for HT-800, whilst Aluminum, lead and zinc ions act as
strong inhibitors.

BENEFITS FOR STARCH SUGAR INDUSTRY

Use of HT-800 can benefit the production of starch sugar as following:

For industrial application of one-time spray liquefaction, first prepare 30 to 35% w/w starch slurry, adjust pH 5.4 to 6.0, and then add this product at a
dosage rate of 0.20 to 0.30 kg/TDS.
Liquefy at 105 to 107°C for 5 to 7 minutes, flash cooling down to around 97°C, keep on liquefying at temperature above 95°C for 90 to 120 minutes so as to reach required DE value.

In case the starch content is above
35%, recommended dosage rate is 0.30 to 0.4kg/TDS.

Exact dosage rate of HT-800 depends on the actual processing
conditions such as starch substrate, liquefaction equipment, pH, temperature,
processing time, etc. To optimize the dosage, it is
recommended to conduct a number of liquefaction trials prior to routine use of
this product.

Typical shelf-life is six months if this product is
stored below 25°C in its original packaging, sealed and unopened, protected
from direct sunlight. Its shelf-life will be eighteen months if it is stored
properly at 0∼10°C. HT-800 has been
formulated for optimal stability. Prolonged storage and/or adverse conditions
such as higher temperatures may lead to a higher dosage requirement, and should
be avoided.

SAFE HANDLING OF ENZYMES

Inhalation of enzyme
dust and mists should be avoided. In case of contact with skins or eyes,
promptly rinse with water for at least 15 minutes. For detailed handling
information, please refer to the Safety Data Sheet.