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Update

IlvE- strain came from Yale (the strain that will overproduce leucine when tyrB is overexpressed). Also, the leucine-overproducing plasmid that when transformed overproduces leucine in cells grown on media supplemented with ammonium formate.

To Do Today

1. Run time courses for J45120 and J45181 (n:53 (J45120) and n+1:13 (J45181) for n=12 through n=8 for addition of SA, for n=1 through n=9 for GC extraction)

2. Add known methyl salicylate to random cells and GC extract- DONE

3. Run the methyl salicylate samples on the GC for recalibration- DONE

4. Contact Li Li about good time to meet about recalibration- DONE

5. Smell cultures grown on Tom media- growing up very, very slowly or probably not at all- contact Tom- DONE

6. GC extract samples grown on Tom media- wait until they grow up more tomorrow