Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Temperature: 33°CRecommendations for Infection: For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, while rocking continuously. End adsorption by adding virus growth medium.Incubation: 4-6 days at 33°C in a humidified 5% CO2 atmosphere, rocking continuously, until CPE is progressed through 80% of the monolayer.

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.