ab41684 staining mouse heart and rat thymus tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in sequential peroxidase and protein block (prediluted) for 20 minutes at 20°C. The primay antibody was diluted 1/100 and incubated with the samples for 45 minutes at 20°C. A HRP-conjugated goat anti-rabbit anitbody was used as the secondary.

Immunocytochemistry/ Immunofluorescence analysis of HepaRG cells labeling Cyclophilin A with ab41684 at 1μg/ml. Cells were fixed with formaldehyde and permeabilized with 0.2% Triton X-100 in PBS. The cells were blocked with 1% milk for 20 minutes at room temperature. A polyclonal goat anti-rabbit Alexa Fluor 488 secondary antibody was used at 1/400 dilution.

Samson AL et al.Physicochemical properties that control protein aggregation also determine whether a protein is retained or released from necrotic cells.Open Biol6:N/A (2016).
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ab41684
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