Some reports show that rodents can acquire aversion to the taste stimulus paired with LPS, which induces inflammatory reaction. However, the brain mechanisms in the conditioned taste aversion (CTA) with immune reactions remain obscure. Therefore, to elucidate the brain mechanism in the retrieval of CTA with LPS, we tried to detect the brain activities using a manganese-enhanced MRI. C57BL/6 male mice were conditioned by i.p. injection of saline, LiCl, or LPS, after drinking saccharin solution. On the test day, all mice were given saccharin solution and scanned by 11.7 T MRI. Our results showed that the signal intensity of the amygdala in the LPS- and LiCl-groups were higher than that in the Saline-group. The signal intensity of the dorsomedial hypothalamus (DMH) only in the LPS-group was higher. The DMH has a role of the body temperature control. Therefore, there is a possibility that the DMH might have an important role in the relationship between taste preference and immune system.