Tag Archives: post-column derivatization

The types and amounts of sugar in animal feeds are as important as the amount of protein, minerals and fats in the determination of nutritive value. We developed a simple and sensitive HPLC method for analyzing six sugars in animal feeds – Sucrose, Fructose, Glucose, Galactose, Maltose and Lactose. Post-column derivatization reagents convert reducing and non-reducing sugars into fluorescent derivatives, which greatly improves the sensitivity and selectivity of the detection.

A quadratic calibration curve with correlation > 0.999 is observed for monosaccharides such as Fructose, Glucose and Galactose. A linear calibration curve with correlation > 0.999 is observed for disaccharides such as Maltose, Lactose and Sucrose. Examples of calibration curves presented in Fig. 1 and Fig. 2.

As of right now, Pickering Laboratories, Inc. will remain open during the “Shelter in Place” order for Santa Clara County so as to provide other essential businesses with the support and supplies necessary to operate, including hospitals and clinical laboratories.

Our inventory levels and response times are currently normal. Responsiveness is subject to change and orders may need to be prioritized accordingly to supply essential businesses.

Thank you for your patience and for your business as we all work together through this difficult time. Please contact us with any questions you may have.