ELISA: In combination with capture antibody, anti-LH α mAb LH-1 (ARG10177), the antibody can be used for LH detection in sandwich ELISA application.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form

Liquid

Purification

Protein G affinity purified

Buffer

0.01M PBS (pH 7.2) and 50% Glycerol

Stabilizer

50% Glycerol

Storage instruction

For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

In the female menstrual cycle, during a 24-48 hour period, luteinizing hormone (LH) is rapidly released from gonadotropic cells in pituitary gland as a result of positive feedback regulation by the elevated estrogen during follicle maturation. The LH surge triggers the release of mature egg from ovarian follicle and the development of remaining follicle into corpus luteum. Progesterone secreted by corpus luteum is needed for preparing the endometrium for egg implantation. LH is critical for ovulation in women. In men, LH stimulates testis to produce testosterone. LH has a α subunit that is identical to the one in other glycoprotein hormones such as TSH, FSH and HCG. LH level is used for predicating ovulation in women to increase the chance of insemination.

Function

Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. [UniProt]