A small RNA (DI RNA), approx. 400 bp in length, was found in plants inoculated with cymbidium ringspot tombusvirus (CyRSV). DI RNA contained sequences derived from at least three regions of the CyRSV genome and was encapsidated in CyRSV protein. DI RNA was formed in plants inoculated with purified genome-size RNA and a double-stranded form was detected in dsRNA extracted from infected tissue. Symptoms of CyRSV infection in Nicotiana clevelandii were less severe when DI RNA was present in the inocula used suggesting that DI RNA interferes with symptom expression.

Validated antibodies in this article

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Annual Conference 2018 10-13 April – ICC Birmingham UK

Attracting over 1,400 delegates, this event is Europe’s largest annual gathering of microbiologists.

The Fundamentals of Cell Culture: Key Principles and Practice

This four day course run by the European Collection of Authenticated Cell Culture (ECACC) aims to deliver a balance of theory, essential techniques and best practices covering the entire cell culture workflow and is particularly relevant to virologists using cell culture in their research.
The course is held at the European Collection of Authenticated Cell Culture (ECACC) laboratories located at the Public Health England site in the south west of England.