Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.

Catalog #

P9004-05E

Applications

Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution

ELISA: 1:50-1:100

Western Blot: 1:50-1:100

Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml

Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Concentration

As reported

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Purity

Purified by ammonium sulfate precipitation.

Immunogen

Synthetic peptide corresponding to human PRMT1 at C-terminal (KLH).

Specificity

Recognizes human PRMT1.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.