Removing genomic DNA contamination from RNA preparations is easy with the enzyme-resin combination in the DNase Max Kit. The highly purified DNase I enzyme is formulated for long term stability at room temperature. This proprietary enzyme removes up to 30 µg of DNA in 20 minutes and is shelf stable for up to 6 months at room temperature. At 4°C the enzyme will remain stable for up to 2 years without loss of activity. Additionally, the DNase Max Kit contains a novel resin to bind and remove the DNase Max enzyme and divalent cations from the reaction, eliminating the need for heat-treating or EDTA inactivation of the DNase.

Resulting RNA is ready to use in downstream applications immediately after resin treatment.

The RNeasy PowerSoil Total Kit was used to extract RNA from two different soils. The resulting product was then treated with the DNase Max Kit to remove any lingering DNA. Genomic DNA was effectively removed from the RNA samples, as seen in the agarose gel images.

Figure 2: DNA levels in RNA pre- and post-DNase Max enzyme treatment

Analysis of the RNA samples described in figure 1 using 16S rRNA gene universal primers and a one-step qRT-PCR kit reveals DNA levels before and after treatment. Using 1 µl of the treated or untreated RNA from each sample, qRT-PCR was run. Treatment of RNA samples reduced DNA 5 logs (>15 cycles), placing it below the level of background DNA in the qPCR kit (red line). Assay efficiency is shown by the standard curve (grey lines) to be at 99%.

Figure 3: Complete removal of DNase with DNase Max Removal Resin.

Samples were treated using the DNase Max Kit and a competitor's kit to compare residual DNase activity using a DNase-free certification assay. All manufacturer's protocols were followed. The negative control (lane 5) did not receive DNase. The attached agarose gel results are after a 1 hour incubation at 37°C and an activation period of 5 minutes at 65°C. DNase was successfully removed by the DNase Max Removal Resin (lanes 3-4). DNase remained in the samples treated with competitor's resin (lanes 1-2).

Performance

Features

Specifications

Scale

10 units/µl, removes 30 µg DNA

Storage temperature

Store at room temperature (15-30°C) for up to 6 months or at 4°C until the expiration date