Background & Aims: Zeolite has skin wound healing capacity. However, its efficacy on burn wound healing has not yet been examined. In this study, we investigated the effects of Zeolite-Ag+/Gelatin nanocomposite on the process of third-degree burn wound healing in mice model.Materials & Methods: Ag+- zeolite/gelatin nanocomposite was fabricated by sol-gel method; MTT assay and antimicrobial activity evaluation of the nanocomposite were performed. Third degree burn wound were created on 40 Balb/c mice (n=10) wound per group and treatment administered daily starting on the day of wounding. Treatment groups included untreated control, SSD, gelatin and nanocomposite. Daily photographs were taken and change in wound area relative to initial area was calculated. At 7 and 14 days, animals euthanized and skin samples were taken to histopathologicl evaluation (H&E staining). Then, the study groups were evaluated for wound contraction percent and histopathological parameters.Results: According to the results of the present study, nano composite-treated wounds revealed significant wound contraction on days 14 compared to control and gelatin treated groups (P<0.0001). Considering investigated histopathological parameters including re-epithelialization angiogenesis and fibroplasia in mice treated with nanocomposite the rate healing of was significantly accelerated when compared to the other groups.Conclusion: In conclusion, based on the results of the present study, it could be concluded that zeolite-Ag/gelatin nanocomposite offered potential advantages in burn wound healing acceleration and improvement. However, clinical use of this nanocomposite needs more supportive studies.