Beta-2 Microglobulin ELISA Kit Background Information:
Human Beta-2 Microglobulin (B2MG) is an 11.8 kD protein identical to the light chain of the HLA-A, -B, and -C antigen. B2MG is expressed on nucleated cells, and is found at low levels in the serum and urine of normal individuals. B2MG concentrations are increased in inflammatory diseases, some viral diseases, renal dysfunction, and autoimmune diseases. Number of studies are available which explain the interpretation of B2MG serum levels in assessing the status of individuals with various clinical conditions.

Beta-2 Microglobulin ELISA Test Principle:
The Beta-2 Microglobulin ELISA assay allows the quantitative determination of B2MG from serum. In this assay, the B2MG in the samples is bound to an available excess of monoclonal antibodies against B2MG, which are immobilized to the surface of the microtiter wells. After a washing step to remove all foreign substances, the quantification of bound B2MG is carried out by adding an enzyme horseradish peroxidase or HRPO labeled antibody, which also binds to the B2MG. The amount of bound enzyme is directly proportional to the B2MG content. For additional details please refer to the instructions for use.

Product inserts for all Cancer ELISA kits follow a similar method. See the Beta-2 Microglobulin ELISA Kit product insert for specific details on preparation, procedures, quality control, and test result interpretation.