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Description

The Dynabeads® mRNA DIRECT™ Micro Kit is designed for simple and rapid isolation of pure, intact polyadenylated (poly(A)) mRNA. The kit isolates highly purified and intact mRNA directly from cells, tissue, and total RNA samples.

• Obtain extremely pure mRNA in about 15 minutes• Integrate total RNA purification and mRNA enrichment steps• Perform reverse transcription and PCR amplification right on the bead• Use in whole transcriptome library preparation with the Ion Total RNA-Seq Kit v2.0

Rapid Isolation of High Purity mRNA Directly from Samples The Dynabeads® mRNA Direct Kit contains magnetic beads for the isolation the mRNA transcriptome from a wide variety of samples. Isolation of intact mRNA is possible thanks to RNase inhibitiors in the Lysis/Binding buffer, combined with stringent hybridization and washing steps. Ribosomal RNA and small RNA molecules (transfer RNA, microRNA, small nucleolar RNA, and small cytoplasmic RNA) do not bind to the beads and are eliminated from the preparation. Only polyadenylated RNA species (mRNA) are captured, resulting in cleaner preparations and more sensitive results. Within minutes, pure mRNA is isolated and ready for use in downstream applications.

A Straightforward Enrichment Procedure The Dynabeads® mRNA DIRECT™ Micro Kit uses a robust affinity purification principle for the enrichment of polyadenylated mRNA. Superparamagnetic Dynabeads®, coupled to oligo-(dT)25, are first equilibrated with Lysis/Binding Buffer, and then mixed with cell or tissue lysate in order to bind mRNA. The beads are then washed to remove contaminating RNA species, and then mRNA is eluted in as little as 5 µl of 10 mM Tris-HCl. The entire process is facilitated by the use of a neodymium magnet (purchased separately), which allows for the quick and efficient immobilization of the magnetic beads during buffer changes.

Designed for a Broad Range of Sample Types Nearly any biological sample of vertebrate, invertebrate, plant, fungal, or bacterial origin can be used with the Dynabeads® mRNA DIRECT™ Kit. The kit is also ideal for extracting mRNA and viral polyadenylated RNA from blood, serum and sputum. Nucleic acid preparations, including total RNA and unfractionated nucleic acid samples, as well as formalin-fixed, paraffin-embedded (FFPE) tissues, may also be extracted using the Dynabeads® mRNA DIRECT™ Kit.