There are numerous methods for measuring 5-hydroxymethylcytosine (5-hmC) in DNA hydroxymethylation studies. The techniques can vary from complicated procedures such as Mass Spectrophotometry which require a significant amount of expertise to simpler ELISA-based methods suitable for a wide range of scientists from all levels of expertise and varying disciplines.

Comparing different samples and assessing 5-hmC can give researchers insight into numerous diseases and other epigenetic outcomes. Measuring global 5-hmC, for example, could give researchers more information about cancer development or the formation of neurological disorders. Reduction in total 5-hmC has been linked to several different cancer types and to the progression of major disorders. However, there is still much to be discovered about 5-hydroxymethylcytosine and its function is not yet entirely understood. Use these kits to unlock the mysteries behind this profound epigenetic mechanism.

DNA hydroxymethylation

5-hmC is an important modified form of cytosine that is involved in the methylation cycle. DNA hydroxymethylation has recently been discovered as an epigenetic mechanism that can impact gene expression and other important biological processes.