Restriction Fragment Length Polymorphism (RFLP)

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Transcript of Restriction Fragment Length Polymorphism (RFLP)

Restriction FragmentLength Polymorphism (RFLP) Definitions Polymorphism: any difference in DNA sequence, coding or noncoding, that can be detected between individuals Restriction Fragment Length Polymorphism: a technique in which DNA regions are digested using restriction endonuclease(s) and subjected to radioactive complementary DNA probes to compare the difference in DNA fragment lengths between individuals Genomes of individuals from the same species are said to be polymorphic unless they are identical twins. Southern Blotting: a procedure that allows DNA in an electrophoresis gel to be transferred to a nylon membrane while maintaining the position of the DNA band fragments Hybridization: complementary base pairing between strands of nucleic acids via hydrogen bonding Autpradiogram: gel pattern imprinted on X-ray film by radioactive probes Where do they occur? When are they useful?

The gel is subjected to a chemicalthat causes the double-starnded DNAto be denatured into a single-strand DNA. Genetic screeningCrime scene investigationAnalysis is a vital genomemapping and genetic disease analysis Utilization A useful research tool for tracing human ancestory, studying wildlife migration and and evolutionProvide useful information for medical or clinical purpose, for example, using to information to detect that allele that causes a genetic diseaseAnalyze the blood, etc. from crime scene to develop the whole genome of an individual as each individuals genomes is different (polymorphism)Identify individuals with specific mutations; for example, a person with sickle cell anemia will have a different allele of a specific gene comparing to a person who doesn't have sickel cell anemia. - occurs in a lab where scientists analyze DNA samples- it is commonly used in scientific areas including genetics and forensic How is it performed? The End