*SYBR green and SYPRO red stains must be disposed of as hazardous waste in separate waste streams. The SYPRO red is mixed with trichloroacetic acid and thus should be marked as ignitable and corrosive and the SYBR green is mixed with DMSO and should be marked toxic. (Kathy Gilbert, EHS)

+

*''SYBR green and SYPRO red stains must be disposed of as hazardous waste in separate waste streams. The SYPRO red is mixed with trichloroacetic acid and thus should be marked as ignitable and corrosive and the SYBR green is mixed with DMSO and should be marked toxic.'' (Kathy Gilbert, EHS)

Use standard 12μL of 2-log ladder with orange G dye? Seems to bleed a bit into acrylamide. Drop the loading volume down to 2 μL. Then the ladder will give an intensity more comparable to the 50ng probe but it still bleeds a bit.

Notes

This kit is not sensitive enough for a complex mixture of protein and RNA. This kit has most optimum results when used with a more purified sample. The analysis of a complex solution with low concentrations of the actual target molecule requires more sensitivity than fluorescence can provide. From Molecular Probes technical assistance.

Safety

SYBR green and SYPRO red stains must be disposed of as hazardous waste in separate waste streams. The SYPRO red is mixed with trichloroacetic acid and thus should be marked as ignitable and corrosive and the SYBR green is mixed with DMSO and should be marked toxic. (Kathy Gilbert, EHS)