The gene stlA encodes a phenylalanine ammonia-lyase that is involved in the production of a stilbene antibiotic in Photorhabdus luminescens TT01.

Williams JS, Thomas M, Clarke DJ.

Molecular Microbiology Laboratory, Department of Biology and Biochemistry, University of Bath, UK.

Photorhabdus is a genus of Gram-negative bacteria from the family Enterobacteriaceae. Members of Photorhabdus have a complex life cycle during which the bacterium has a pathogenic interaction with insect larvae whilst also maintaining a mutualistic relationship with nematodes from the family Heterorhabditidae. During growth in the insect, Photorhabdus bacteria produce a broad-spectrum antibiotic identified as 3,5-dihydroxy-4-isopropylstilbene (ST). The biochemical pathway responsible for the production of this antibiotic has not been characterized. In this report, a mutant strain of Photorhabdus luminescens subsp. laumondii TT01, BMM901, has been isolated, by transposon mutagenesis, that is unable to produce the ST antibiotic. Using in silico studies, feeding experiments and biochemical analyses, it is shown that the gene mutated in this strain, stlA, encodes phenylalanine ammonia-lyase (PAL). PAL catalyses the non-oxidative deamination of l-phenylalanine to trans-cinnamic acid and the enzyme is ubiquitous in plants, where it is involved in the production of phenylpropanoids such as lignin and phytoalexins. However, this is the first report of PAL activity in a member of the Proteobacteria.

Randy, nope...not been to your site, I will check it out. I used to post in '04 & early '05 on nuspa before I changed my strategic route plan. So you remember her huh...she's a fantastic lady...we have alot in common. I'll be off for that afternoon. Washer broke, clothes to the walls and ceiling, groceries still in the car, yadda, yadda, yadda. So Randy...have you ever considered or know much about flukes? Their top of my list...and "V's" too.

London...brain tired...never. We can't have that!? Happens to me like every three days, then I've got to take a serious break. So London...teacher?

London...I can't open word office documents. I can't find my original paperwork that came with this computer to get the code to get my temp. offer. I'll find it...it's in the messy garage somewhere. I'm sorry about your feet...I hate this disease. Was it here, yeah, Randy was saying killing you slowly...yes, I agree. One other reason why we don't get the attention we require...so we can live the rest of our lives miserable and in ill health because they don't have a clue. John hit it...we are lucky...we have the fibers and the skin feelings, we know what's killing us...the rest of the plantet doesn't really have a clue!?

Wonder why you can't open them- Maybe your computer came with a freet trial of Micro Word doc....mine did, but it will still open> if this is what is happening to yours then if/when that box pops up (the 2nd dialog
box...just hit cancel. It should still open up) Yet I can not now copy anything in word, yet I can open the documents.