5′-Thiol Modifiers are phosphoramidites used to produce a functional thiol group on the 5′-terminus of oligonucleotides. Thiol modification allows attachment of a variety of products, such as fluorescent dyes, maleimide compounds, biotins and proteins. The trytyl group is used to protect the thiol and is not acid labile. The trityl protecting group which is lipophilic allows a trityl-on reversed-phase purification. A final deblocking of the oligonucleotide is accomplished by oxidation with silver nitrate and dithiothreitol (DTT).

Technical Information

Molecular Formula: C34H45N2O2PS

Molecular Weight: 576.78 g/mol

Specification:

HPLC – Identity is confirmed by comparison with a reference sample, Minimum purity

NMR – Purity is determined by 31P NMR and isomeric purity is veified by 1H NMR