Norgen’s Microm DNA Isolation Kit is designed for the rapid preparation of genomic DNA from filters used for air sample collection. Various air-borne microorganisms may be trapped on the filter, including bacteria, fungi and viruses. Genomic DNA is efficiently extracted from the filter by a combination of heat treatment and a unique lysis buffer. PCR inhibitors are effectively removed during the washing steps and the purified genomic DNA is ready to use for downstream applications such as PCR and microarray analysis. The option of bead beating is also provided in order to allow for improved DNA yields for certain fungal and yeast species captured on the filter. Preparation time for ten samples is less then 30 minutes, and each kit contains sufficient materials for 50 or 100 preparations.

Kit Specifications - Spin Column

Sample Type

Dried or preserved filter

Maximum Column Binding Capacity

10 μL

Maximum Column Loading Volume

650 μg

Minimum Elution Volume

20 μL

Size of DNA Purified

All sizes

Time to Complete Purification

20 minutes

Kit Specifications - 96-well

Sample Type

Dried or preserved filter

Maximum Binding Capacity

50 μL

Maximum Loading Volume

750 μg

Size of DNA Purified

All sizes

Time to Complete 96 Purifications

50 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers.