Introduction. Cervical twin ectopic pregnancy after IVF-ET is rare and catastrophic complication. However, here is no consensus on the best treatment strategy. Patient and method. Case report of cervical twin ectopic pregnancy after IVF-ET treated by transvaginal ultrasound-guided aspiration plus systemic single injection of methotrexate, which followed by full-term delivery in next IVF-ET cycle. Conclusion. Transvaginal ultrasound-guided aspiration and systemic methotrexate administration can be safely and easily used to treat cervical ectopic pregnancies and to preserve the fertility of the patient without any major complications.