AlphaLISA SureFire Ultra p70 S6K pT389 Lysate

Extra positive and negative control lysates for the AlphaLISA SureFire Ultra phospho-p70 S6K (Thr389) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-p70 S6K assay kit, but can also be ordered separately.

This control lysate is intended for use with the AlphaLISA SureFire Ultra phospho-p70 S6K (Thr389) assay kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. This control lysate was prepared from MCF7 cells, cultured to confluence in T175 flasks in 10% FBS containing medium for 3 days, then treated with insulin (500 µg/mL) for 30 min and lysed in 30 mL of 1X SureFire Ultra Lysis buffer.