Abstract

Background & Purpose: Male infertility is a significant global health issue, in 40% of cases asthenozoospermia is the leading cause. ICSI is the primary method of ART however; this does not defer the male infertility enigma. Human sperm cells are comprised of complex signalling systems that make motility diverse and adaptive. Identifying a molecular target in spermatozoa and utilising compounds to improve cell function is desirable. This study aims to identify compounds that increase [Ca2+]i via CatSper, a fundamental ion channel for motility.

Conclusion and Implications: Extensive examination of trequinsin hydrochloride has shown novel pharmacological actions that stimulate cell hyperactivation and penetration. Utilising robust screening strategies and identifying specific molecular targets could provide insight into the intracellular regulation of normal and impaired sperm and may help to establish an alternative diagnostic treatment to ICSI for patients who suffer from reduced sperm cell motility.