Rat IgG2A Allophycocyanin Isotype Control Summary

Selected for use as rat IgG2A isotype control for flow cytometry applications.

Source

Monoclonal Rat IgG2A Clone # 54447

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

KLH

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin

Applications

Recommended Concentration

Sample

Control

Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P(fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application.
General Protocols
are available in the Technical Information section on our website.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

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Mass (in vial)

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Desired Reconstitution Concentration

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2A Fc

Alternate Names

IgG2A

Product Datasheets

Citations for Rat IgG2A Allophycocyanin Isotype Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.

Freshly isolated and 48Hrs stimulated human PBMCs using anti-human CD3 were stained with the Rat IgG2A Ab Isotype and TIM-3 Ab with similar background. The isotype control has been very consistent between runs and helps decide where the gated should be started for the test antibody.