Meh don't know if this proves anything. Bradford/Lowry assay determines the ammount of protein present by binding the coomassie dye to the tertiary structure of the protein (wikipedia). If APW is really pure hydrolized whey as dym-100 is there should only be amino acids present. Amino acids are the Legos that build protein they are not themselves protein. I would be very curious to see the results if they ran dym-100 along side APW. Not defending the stuff just don't think this is conclusive.

Meh don't know if this proves anything. Bradford/Lowry assay determines the ammount of protein present by binding the coomassie dye to the tertiary structure of the protein (wikipedia). If APW is really pure hydrolized whey as dym-100 is there should only be amino acids present. Amino acids are the Legos that build protein they are not themselves protein. I would be very curious to see the results if they ran dym-100 along side APW. Not defending the stuff just don't think this is conclusive.

Theoretically, yes. But if the whole protein was lysed then the hydrophobic amino acid residues should interact with the binding capabilities of the dye. The A280 is a much more believable test since it would actively measure aromatic ring structures in protein. Even if it was a hydrolysed protein, it should contain equivalent amounts of those residues, and a 5-10x difference seems disconcerting.

I didn't use the wrong test. It says in the Reddit thread that I used a detergent-compatible Lowry assay, not a Bradford one. The Lowry assay works under strongly alkaline conditions, so it doesn't depend on the protein's tertiary structure. I also took absorbance measurements @ 280 nm. Both these would work fine on amino acids or proteins. There's no reason why the APW powder should have failed both those tests when the ON whey passed them if it really had as much protein in it as it says it does.

I didn't use the wrong test. It says in the Reddit thread that I used a detergent-compatible Lowry assay, not a Bradford one. The Lowry assay works under strongly alkaline conditions, so it doesn't depend on the protein's tertiary structure. I also took absorbance measurements @ 280 nm. Both these would work fine on amino acids or proteins. There's no reason why the APW powder should have failed both those tests when the ON whey passed them if it really had as much protein in it as it says it does.

American Pure Whey even created a fake lab testing for "proof" their protein is legit.

Bad company.

Originally Posted by shanghei

I cant win, you have 27k posts.

Originally Posted by realrthanu

Meh don't know if this proves anything. Bradford/Lowry assay determines the ammount of protein present by binding the coomassie dye to the tertiary structure of the protein (wikipedia). If APW is really pure hydrolized whey as dym-100 is there should only be amino acids present. Amino acids are the Legos that build protein they are not themselves protein. I would be very curious to see the results if they ran dym-100 along side APW. Not defending the stuff just don't think this is conclusive.

Originally Posted by copperions

I didn't use the wrong test. It says in the Reddit thread that I used a detergent-compatible Lowry assay, not a Bradford one. The Lowry assay works under strongly alkaline conditions, so it doesn't depend on the protein's tertiary structure. I also took absorbance measurements @ 280 nm. Both these would work fine on amino acids or proteins. There's no reason why the APW powder should have failed both those tests when the ON whey passed them if it really had as much protein in it as it says it does.

And I'm not a shill, though I totally understand why it looks that way. I'm not repeating anything that isn't already in the Reddit thread about this. This would also be an odd way for someone to shill for ON, although I don't really think their reputation needs boosting by some random internet person with one post (now two posts). I just used it because it's what I had on hand, it's reputable, and I needed a whey product to compare the APW whey to. The BSA standard alone was insufficient since it reacts to the assays a bit differently than whey.

And I'm not a shill, though I totally understand why it looks that way. I'm not repeating anything that isn't already in the Reddit thread about this. This would also be an odd way for someone to shill for ON, although I don't really think their reputation needs boosting by some random internet person with one post (now two posts). I just used it because it's what I had on hand, it's reputable, and I needed a whey product to compare the APW whey to. The BSA standard alone was insufficient since it reacts to the assays a bit differently than whey.

cool man.....nice work. How much did the test cost to do? I want to know if my dymatize is legit( although im sure it is).

I still have a few scoops left of the 3 tubs i bought from APW..strong waste of monies.

I didn't use the wrong test. It says in the Reddit thread that I used a detergent-compatible Lowry assay, not a Bradford one. The Lowry assay works under strongly alkaline conditions, so it doesn't depend on the protein's tertiary structure. I also took absorbance measurements @ 280 nm. Both these would work fine on amino acids or proteins. There's no reason why the APW powder should have failed both those tests when the ON whey passed them if it really had as much protein in it as it says it does.

Sorry I may have missed but which ON whey did you use was it is hydro whey or gold standard?

cool man.....nice work. How much did the test cost to do? I want to know if my dymatize is legit( although im sure it is).

I still have a few scoops left of the 3 tubs i bought from APW..strong waste of monies.

The modified Lowry assay kit (DC protein assay from Bio-Rad) is a few hundred bucks, but it's good for LOTS of tests. A guy on Reddit has offered to have people send him their protein powder so he can test it. I can't link to it since I only have two posts, but just Google "offering broscience service"; it's the first hit.

Someone's sending him Dymatize.

Originally Posted by realrthanu

Sorry I may have missed but which ON whey did you use was it is hydro whey or gold standard?

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