Introduction to Fluorescence

Selected Literature References

The field of fluorescence spectroscopy and microscopy is experiencing a renaissance with the introduction of new techniques such as confocal, multiphoton, deconvolution, time-resolved investigations, and total internal reflection, coupled to the current advances in chromophore and fluorophore technology. Green Fluorescence Protein is rapidly becoming a labeling method of choice for molecular and cellular biologists who can now explore biochemical events in living cells with natural fluorophores. Taken together, these and other important advances have propelled the visualization of living cells tagged with specific fluorescent probes into the mainstream of research in a wide spectrum of disciplines. The reference materials listed below were utilized in the construction of the introductory fluorescence section in the Molecular Expressions Microscopy Primer.