Beta-Tubulin III belongs to the tubulin family. Tubulin is the major constituent of microtubules. It forms dimers of alpha and beta chains. One mole of tubulin binds to two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. beta-Tubulin III contains a highly acidic C-terminal region which can bind cations such as calcium. It is abundant in the central and peripheral nervous systems.Rabbit Anti-beta-Tubulin III Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide within residues 150-200 of human beta-Tubulin III protein (Swiss Prot: Q13509).

Application Details

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/mL. Western blot: 0.5-1 µg/mL. Immunohistochemistry: 5-10 µg/mL. Flow cytometry: 1-3 µg for 1 x 10E6 cells. Other applications: user-optimized.

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage

4 °C/-20 °C

Storage Comment

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.