Anti-Interferon beta antibody (Biotin) 图像

ICC/IF image of ab84258 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84258, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Thank you for contacting Abcam. I have gone ahead and added the discount code in the additional notes section of the Abreview you submitted. You can now go ahead to use the discount code to receive your free antibody. If there is anything els...

I do understand your concerns. It may be that you would want to run a western blot with this product to ensure that it is picking up IFN from your cells, it would them be possible to run a purified protein as control...

I think these two antibodies will work together in a sandwich ELISA, but we have not tested them together. You will need to test a few different dilutions to achieve the optimal signal with the least background. ...

Thank you for contacting Abcam. I have heard back from the lab and they have told me that the biotin is covalently coupled to primary amines (lysines) of the immunoglobulin and not to either the N or C terminal. If there is anything else I can help you...