Greetings, scientists,
I am desperately searching for a reliable immunostaing protocol for the
detection of Macrophage in frozen sections of Mouse arteries. None of the
antibodies or protocols I have tried have worked. If anyone has a method
that works or knows of one, please let me know. Our lab is studying
atherosclerosis in mouse models, and obviously the macrophage is a star
player. I'm stuck until I can reliably identify and quantitate these cells
in atherosclerotic lesions.
Thanks in advance, with more to follow if I get this protocol working.
Jerry