Protein A and Protein G bind specifically to Fc regions of many mammalian immunoglobulins. Protein A and Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological fluids. These reagents are also commonly used to capture immune complexes generated in immunoprecipitation experiments. Because Protein A and Protein G vary widely in their abilities to bind individual immunoglobulins, consult the table below before proceeding with your application. It should be noted that the Fc binding characteristics of naturally produced Protein A and Protein G are identical to their recombinant counterparts. However, also note that Zymed’s recombinant Protein G products are deleted for two albumin binding sites present in the wild type molecule. For certain procedures, it may be advantageous to use a light chain binding reagent (e.g. Zymed’s Kappalock products) instead of Protein A or Protein G

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