Introduction: The estimation of blood loss rate during surgery for prediction of transfusion of blood or blood products requirement is important for surgeon and anesthesiologist. In regard to various results and lack of definite index for start of transfusion in operating rooms, the assessment of blood loss reduction is necessary. The aim of study was evaluation of hemoglobin (Hb) value and hemorrhage estimation rate. Methods and materials: 48 cases underwent major orthopedic surgery with high probability of intraoperative blood loss rate was included in study. Hb, hematocrit (Hct) and basic vital signs were measured preoperatively and blood loss rate was estimated by using of blood volume in suction, bloody gases and blood loss in operation field and recorded. The blood sample per 100 mL blood loss was sent to laboratory. For transfusion deciding based on Hb, we did not any intervention. Results: 26 males and 22 females were included in study. Our results showed that Hb value of males was higher significantly of females in preoperative period. After beginning of blood loss, Hb and Hct values declined with fixed slope and there was high negative correlation between them. First, intraoperative systolic and diastolic pressures declined with high slope and then reduced with mild slope. Hb value reduced significantly per 100 mL blood loss. During survey, no alteration in urine output did not be observed. Conclusion: Present study showed that much more intraoperative blood loss could reduce Hb with high correlation coefficient and measurement of it is efficient on transfusion.

Currently, transfusion is an important challenge in operating room. Transfusion is usually a result of intraoperative blood loss. During some of surgeries, this practice is not performed proportionally and correctly. Specialists have various opinions about this. This discrepancy leads to frequent and sometimes, unnecessary transfusions [1] [2] . Over decades, evidences showed that transfused patients expose with different adverse events (particularly, allogeneic blood) [1] [2] [3] [4] [5] such as postoperative pneumonia, sepsis and death [3] . American association of blood bank (AABB) recommended restricted transfusion [4] and many authors strictly appreciated on transfusion when Hb < 8 gr/dL (i.e. Hb > 10 gr/dL is not suitable for transfusion) [5] [6] . However, estimation and prediction of exact amount of blood loss is very important, more exact estimation could help surgeon and anesthesiologist for overcoming on possible risks during procedure [7] . Up to date, any definite indicator had not been found that could determinate what patient would need to transfusion and how much blood would require? Overall, transfusion is controversy. Main reason is referred to lack of specialty acceptance of guidelines because they are not based on evidences [8] . Therefore, the assessment of transfusion amount reduction strategies is necessary in regard to several results and absence of definite index for start of transfusion in OR. The aim of present study is evaluation of Hb value and estimation of amount of blood loss.

2. Methods and Material

This descriptive clinical trial was conducted on patients (ASA 1&2) undergoing major orthopedic procedure with probability of intraoperative bleeding. The study was approved by ethical committee of Tabriz University of medical sciences and faculty of medicine; and informed written consent was obtained. All the patients during designed study time were included. In regard to lack of same studies, we used results of pilot studies for calculation of sample size. In 12 cases, the presence of association and its severity between intraoperative Hb and need to blood transfusion were assessed and evaluated. Then, sample size was determined to 48 cases with considering α = 0.05, 80% power and acceptable difference of P-value < 0.05. Patients with known cardiopulmonary disease, risk of insufficient oxygenation of tissues, and patients with Hb < 10 gr/dL were excluded from study [5] . We did not transfuse any blood preparation during surgery and if, we excluded them from study. Selected patients received general anesthesia after premedication with fentanyl and midazolam. Anesthesia was maintained by 2 to 3 mg/kg propofol and 0.5 mg/kg atracurium and then, patients were intubated with proper size of endotracheal tube. In preoperative period, Hb and Hct values were routinely measured. Monitoring composed of non-invasive blood pressure measurement, heart rate, electrocardiogram, body temperature and urine output was performed. In intraoperative period, amount of blood loss was estimated by blood volume in suction, bloody gases and bloodshed in operation field in 4 stages and recorded. At the same time, a blood sample was sent to the lab to measure hemoglobin in proportion to a specific volume of bleeding with a 100-mL frequency. For deciding to transfusion, we did not intervene based on Hb value and only the amounts of collected Hb on the basis of amount of bleeding at the same time were investigated for possible association of bleeding severity and probability of using it as an indicator for possible decision making for transfusion at the end of the study. Given data were analyzed by descriptive statistical methods (frequency, percentage and mean ± standard deviation) and comparison test for independent groups and repeated measurements scheme test (Mauchly’s test) and regression with SPSS 0.16. P-value less than 0.05 was considered statistically significant.

This general mode scheme is a paired test. With the difference that instead of comparing a group in 2 situations, one group is compared in two or more situations. When the same measurements are taken for a few times by a subject, the analysis of variance with repeated measurements should be used to analyze the data and compare the mean of data between these groups. The default test is used for repeated measurements of the Mauchly’s Test of Sphericity.

3. Results

48 cases (26 males and 22 females) with mean age of 43.34 ± 20.59 years old were included in study. In preoperative period, we measured variables such as blood pressure, heart rate, Hb and Hct values as basic measures which were considered as scale for further evaluations to have basis for comparison of upslope or downslope trend of these variables in postoperative period. We summarized preoperative Hb and Hct values in Table 1.

In given time interval to reach given blood volume, Hb value had no significant difference in relation to preoperative period. Although in other cases, mean Hb value had significant difference between 2 stages (i.e. significant decline of Hb mean value was associated with increased bleeding volume) (Table 2). Hct value, the

same as Hb value, had no significant difference with preoperative period until 100 mL bleeding volume. But in other cases, mean of Hct value had significant difference (i.e. significant decline of Hct mean value was associated with increased bleeding volume) (Figure 1).

There was no significant difference between heart rate and various bleeding volumes.

At least, mean of systolic/diastolic pressures had significant difference in 4 groups as follows: mean of systolic pressure had significant decline except in 300 mL bleeding volume. On the other hand, comparisons demonstrated that slope of decline trend of mean diastolic blood pressure was gradually slow (Figure 2).

One of intraoperative challenges, especially in orthopedic surgeries is blood loss. Present study showed that when blood loss reached to 100 mL at mean of 29 minutes of procedure and along with, Hb values started to decline. Despite of higher Hb values in males before surgery, Hb and Hct values started to decline with fixed slope in two genders. Accordingly, Hb value after 300 mL blood loss reduced 1.5 unite in relation to basic measures. Therefore, we can predict possibly when patients will need to transfusion if we have basic Hb value. Robinson et al showed that intraoperative transfusion was highly related to primary hemoglobin [2] . Patients with lower Hb value had much more requirement to transfusion. So, aged patients and women required transfusion due to lower basic Hb value [2] . Rogers MA et al suggested that women received much more transfusion than men during heart operations [9] and Rummassk and Chen showed gender effect on amount of blood loss and higher transfusion in females [10] [11] . Murray et al used Hct measurement and blood gas analysis for confirmation of estimated blood and fluid loss [12] [13] . They concluded Hct < 25% was w threshold for transfusion [12] . Hemodynamic monitoring is a central component of intensive care. Hemodynamic variables often suggest hypovolemic and etiologies to cardiovascular insufficiency, thus it may be an effective manner in estimation of

blood loss [13] . Present study showed systolic/diastolic pressure reduced during surgery and parallel with blood loss, which seems to be a normal event. Robinson et al showed blood pressure and heart rate (HR) were high before surgery due to probably stress, then HR reduced over time because of induction of anesthesia or reduction of patient’s stress. It is obvious that increased blood loss correlates with increased HR that compensatory mechanisms is to increase cardiac output and maintain perfusion pressure [14] . Also, estimating blood loss is complicated by other factors including urinary losses that help to guide volume replacement, hemorrhage. In this study, slope of urine volume was homogenous; it indicated proper hydration during surgery, so application of hydration guidelines during conventional operations even with major bleeding maintains normal circulation.

5. Conclusion

Hemoglobin concentration may be therapeutic guide in actively bleeding patients and we can predict requirement of transfusion with measurement of Hb values and intraoperative blood loss. Therefore, this practice has positive effect on transfusion.