The TRAF (TNF receptor-associated factor) family is a group of adapter proteins (TRAFs 1-6) that link a wide variety of cell surface receptors to diverse signaling cascades leading to the activation of NF-kB and mitogen-activated protein kinases (reviewed in Chung et al, 2002). TRAFs are major signal transducers for both the TNF and IL- 1:TLR receptor superfamilies and collectively play important functions in both adaptive and innate immunity. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. TRAFs interact with a variety of proteins that regulate receptor-induced cell death or survival, and TRAF-mediated signaling can promote cell survival or interfere with death receptor-induced apoptosis. Human TRAF4 is a 470aa protein.

Catalog #

T9161-59

Applications

Suitable for use in Immunocytochemistry, Immunohistochemistry and Immunoprecipitation. Not suitable for Western Blot. Other applications not tested.

Recommended Dilution

Immunocytochemistry: 1:300-1:800, transfected cells.

Immunohistochemistry (paraffin sections): 1:500-1:1000.

Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Clone Type

Polyclonal

Isotype

IgG

Host

Rabbit

Source

Human

Concentration

Not determined

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

Purity

Serum

Immunogen

Synthetic peptide corresponding to aa1-18 (MPGFDYKFLEKPK

RRLLC) of human TRAF4. This sequence is completely conserved in mouse TRAF4.

Specificity

Recognizes human TRAF4.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.