1. Mate animals (donor) containing genetic region of interest (transgene, knock-out gene, etc.) to animals of recipient strain to produce the F1 generation. Recipient animals must be inbred and homozygous at the locus of interest. All F1 animals will be 100% heterozygous at every locus.

2. Mate F1 animals to animals of recipient strain. We do not use genetic markers on the X or Y chromosomes in our assay therefore it is recommended that the sex chromosomes be fixed early in the breeding scheme for convenience. Mate female F1 animals to male recipient strain animals to fix the Y and generate the N2 generation.

3. Analyze the DNA from 8-10 males who carry the genetic region of interest by microsatellite analysis. We offer genotyping services for performing or developing gene-specific PCR assays to genotype for the genetic region of interest or the client can perform the genotyping prior to sending tail snips for microsatellite analysis.

4. Based on the microsatellite analysis, you choose 2-4 animals that have the greatest percentage of recipient genome and mate these to female recipient strain animals. This will generate the N3 generation and fix the X chromosome in the males.

5. Analyze the DNA from 8-10 males with the genetic region of interest from the N3 generation by microsatellite analysis. Choose the 2-4 males that have the greatest percentage of recipient genome. Continue this selected breeding backcross process through the N5 generation. At this point, >99% of the genome should be derived from the recipient genetic background.

IDEXX BioResearch

IDEXX BioResearch is your strategic partner for reliable, reproducible results while accelerating your research through innovative technologies, logistics support and scientific expertise, so you can confidently enhance the health and welfare of people and animals.

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From highly respected educational institutions to world-class pharmaceutical companies and independent researchers, our partners have come to expect only the best from us—from industry-leading products and services to our world-class team of veterinarians, PhD microbiologists, molecular biologists, and geneticists.

The IDEXX BioResearch Scientists discovered a new pathogen in Immunocompromised Mice - Corynebacterium (HAC2). Which conclusions can we draw at the moment and what does this mean for your facility? Learn more in this on demand webinar.

We're proud to share this highlights video from the 2018 Animal Health Investment meeting in London, organised by Kisaco Research. We are excited to confirm we are participating again in the 2019 event.

In the April issue of the SECAL periodical, IDEXX BioResearch colleague David Mayo had been interviewed on SECAL membership and the 3R's. Read the original interview in Spanish and below the translated English version.

In November 2016 we reported the discovery of a new rat Polyomavirus by IDEXX researchers. Now we can share the peer reviewed publication, which includes additional information regarding the characterization, transmission, and preliminary epidemiologic studies.