User:Maria Briscione/Notebook/Chem 571/2011/09/27

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Objective

Description

In order to synthesize gold nanoparticles, Chloroauric acid (0.25mM) and BSA★ (1.5µM) in 10mL of water added together. A UV-vis spectrum was analyzed at 0 seconds (t0) and after thirty minutes following. The samples were maintained at 70C within the UV-Vis while the reaction proceeded.

The procedure to transform the DNa into cells was as follows: The wild-type DNA was first digested by use of an enzyme, DpnI then thawed on ice for 15 min. The cells were then plated on LB/agar plates (25 g/L broth and 20 g/L agar) and with a 100μg/mL solution of antibiotics. Forty μL of competent cells and 5 μL of DNA were combined and incubated on ice for 30 minutes. The DNA/cell mixture was then heat shocked at 42C for 30 sec, and subsequently incubated on ice for 5 min. 250μL of SOC media was added to the mixture, which was then incubated in the shaker at 37C for 1 hr. 100μL of cells were spread (using sterile techniques) on the LB/agar plate. The plate was then inverted and stored in a 37C oven overnight.

Data

5.81 μL of Chloroauric acid (4.3 mM)

97.4 μL of BSA (15.4 μM)

9844 μL water

(as stated the values of chloroauric acid and BSA that were added did not yield the desired concentration).

Notes

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