Akila JayaramanHarvard MIT Division of Health Sciences and Technology, Singapore MIT Alliance for Research and Technology, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of AmericaPLoS ONE 6:e17616. 2011

Tracheal branching morphogenesis in Drosophila: new insights into cell behaviour and organ architecture

Akila JayaramanHarvard MIT Division of Health Sciences and Technology, Singapore MIT Alliance for Research and Technology, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of AmericaPLoS ONE 6:e17616. 2011

..These findings are significant in the context of monitoring the evolution of the currently circulating 2009 H1N1 viruses...

Tracheal branching morphogenesis in Drosophila: new insights into cell behaviour and organ architecture

..Little is known about the regulation of tissue homeostasis by organ-organ interaction. Here we demonstrate that trachea-derived Decapentaplegic (Dpp), the main bone morphogenetic protein ligand in Drosophila, is essential for adult ..

..Avian influenza virus (AIV) not only causes significant economic losses in poultry production, but also is of great concern to human health. The objective of this study was to identify miRNAs associated with AIV infections in chickens...

..Our data throw light on the mechanisms of generation of pandemic viruses from their avian progenitors and open avenues for cell level-oriented studies on the replication and pathogenicity of influenza virus in humans...

..These findings may explain, at least in part, the localization and severity of H5N1 viral pneumonia in humans. They also identify the cat and the ferret as suitable experimental animals based on this criterion...

Localized Fgf10 expression is not required for lung branching morphogenesis but prevents differentiation of epithelial progenitors

..Airway hyperresponsiveness is a critical feature of asthma. Substantial epidemiologic evidence supports a role for female sex hormones in modulating lung function and airway hyperresponsiveness in humans...

The emergence of shape: notions from the study of the Drosophila tracheal system

..To evaluate the yield of clinical and radiological features in the diagnosis of suspected foreign body aspiration in children and to assess factors associated with delayed diagnosis of foreign body aspiration...

..These data suggest that NE upregulates TGF-beta1 gene expression and release via My88/IRAK/NF-kappaB pathway, possibly through activation of TLR4, and shed light on a potential role of neutrophils in the pathogenesis of asthma...

..which is a disease characterized by a uniform cartilaginous sleeve in place of a normally ribbed cartilagenous trachea. We have studied the cellular and molecular basis of this phenotype in a mouse model of AS (Fgfr2c(+/Delta) mice),..

..Here we show that these metabolites change electrolyte transport by stimulation of P2Y6 receptors in mouse trachea. Immunohistochemistry confirmed luminal and basolateral expression of P2Y6 receptors...

The plakin Short Stop and the RhoA GTPase are required for E-cadherin-dependent apical surface remodeling during tracheal tube fusion

..Although airway abnormalities are common in patients with cystic fibrosis (CF), it is unknown whether they are all secondary to postnatal infection and inflammation, which characterize the disease...

..We addressed this issue by examining intracellular lumen formation in the Drosophila trachea. The main branches of the Drosophila tracheal system have an extracellular lumen because their cells fold to form ..

Defects in tracheoesophageal and lung morphogenesis in Nkx2.1(-/-) mouse embryos

..Failure in septation between the trachea and the esophagus in Nkx2...

In vivo and in vitro morphometry of the human trachea

Kirollos Salah KamelDepartment of Anatomy and Structural Biology, Otago School of Medical Sciences, University of Otago, Dunedin, New ZealandClin Anat 22:571-9. 2009

Most morphometric studies of the human trachea have relied on plain radiographs with their attendant limitations. Reports using computed tomography (CT) have focused on the growing trachea or one particular dimension...

..Since expression of Hoxa-5 is confined to the mesenchymal component of the developing trachea and lung, the effects observed in epithelial cells may result from a disruption of normal epithelial-mesenchymal ..

..Extensive or complete labeling of peripheral lung, thyroid, and thymic epithelia, but not trachea, bronchi, or gastrointestinal tract occurred when mice were exposed to doxycycline from embryonic day (E) 4...

..member of an evolutionarily conserved family of predicted transmembrane proteins, is expressed in the developing trachea. We report that all mice homozygous for a null allele of Tmem16a died within one month of birth and exhibited ..

..FGF signaling guides tracheal cell migration in the embryo. However, misexpression of branchless in the ovary had no effect on border cell migration. Thus border cells and tracheal cells appear to be guided differently...

..However, the extension of tracheal cell processes leading to the formation of the elaborate tree structure is blocked. The DFGF-R protein may thus participate in receiving spatial cues that guide tracheal cell outgrowth...

The steady-state turnover of epithelial cells in the lung and trachea is highly relevant to investigators who are studying endogenous stem cells, manipulating gene expression in vivo, or using viral vectors for gene therapy...

..We now show that wun has an essential tissue-autonomous role in development of the trachea: the catalytic activity of Wun is required to maintain septate junction (SJ) paracellular barrier function, loss ..

Serum-free culture of mouse tracheal epithelial cells

R K KumarSchool of Pathology, University of New South Wales, Sydney, AustraliaExp Lung Res 23:427-40. 1997

..Because the number of cells that can be isolated from the mouse trachea is very small, published techniques are unsuitable for this species...

Lymphocytic infiltration in the chicken trachea in response to Mycoplasma gallisepticum infection

..There was no significant difference in the number of CD8+ cells in the upper, middle and lower trachea. High numbers of both CD4+ and CD8+ cells were found with variable numbers of TCRalphabeta1+ and TCRalphabeta2+, ..

New methods for monitoring dynamic airway tissue oxygenation and perfusion in experimental and clinical transplantation

..Rapid progress in perinatal medicine has resulted in numerous tracheo-bronchial interventions on fetal and neonatal airways. The present study was performed to compile normative data for tracheal dimensions at varying gestational ages...

..capacity of SLIGRL to elevate PGE(2) levels and produce relaxation in isolated segments of upper and lower mouse trachea. SLIGRL produced concentration-dependent increases in PGE(2) levels and smooth muscle relaxation, although both ..

Determination of endotracheal tube size in a perinatal population: an anatomical and experimental study

This study aimed at correlating anatomical dimensions of the larynx and trachea to age and weight in a prenatal population. Endotracheal tube size determination was then proposed according to these considerations.

Pathogenicity of a QX strain of infectious bronchitis virus in specific pathogen free and commercial broiler chickens, and evaluation of protection induced by a vaccination programme based on the Ma5 and 4/91 serotypes

..This study confirms that vaccination at 1 day old and at 14 days of age using the Ma5 and 4/91 IB vaccines may be instrumental in reducing the economic impact of QX IB virus infections in layer and broiler farms...

..Our objective was to demonstrate that PT performed under real-time US guidance with visualization of needle path during tracheal puncture is feasible, including in patients with features that increase the technical difficulty of PT...

The Drosophila Claudin Kune-kune is required for septate junction organization and tracheal tube size control

..Double- and triple-mutant combinations of Sinuous and Megatrachea with Kune-kune resemble the Kune-kune single mutant, suggesting that Kune-kune has a more central role in septate junction formation than either Sinuous or Megatrachea...

The mammalian respiratory system, consisting of both trachea and lung, initiates from the foregut endoderm. The molecular program that instructs endodermal cells to adopt the respiratory fate is not fully understood...

Prenatal and early postnatal morphogenesis and growth of human laryngotracheal structures

Pierre FayouxDepartment of Otolaryngology Head and Neck Surgery, University Hospital of Lille, FranceJ Anat 213:86-92. 2008

..An anatomical prospective study was based on examination of larynx and trachea from 300 routine autopsies of fetuses and infants, free of malformation and never intubated...

..Thus, extracellularly released ATP signals to prevent ozone-induced death and supplementation with ATP or its analogs can augment protection, at least in part via Akt and /or ERK signaling pathways and their metabolic effects...

Morphogenesis of the trachea and esophagus: current players and new roles for noggin and Bmps

Research Grants64

..To test the hypothesis that MCC is reduced in aged mice. Mucociliary clearance will be measured in the trachea and nasopharynx of 3, 6, 12, and 24 month old C57BL/6 mice by tracking the movement of endogenous mucus particles ..

..that determines both the patterning and differentiation of airway epithelial cells (AECs) lining the developing trachea and bronchi, and the formation of submucosal glands (SMGs) that secrete the majority of fluids, electrolytes, and ..

..aim 1, we will map the cells that produce these chemokines in the various mucosal tissues (oral cavity, bronchus, trachea, esophagus, gut and the female reproductive tract) by immunohistochemistry and/or in situ hybridization...

..onset of UVP in horses suggested that chronic compression to the RLN occurred near the aortic arch or inferior trachea. In fact, recent research has shown that individuals with idiopathic UVP may have damage to the RLN at the level ..

..of propelling some cells like sperm and protozoa through a liquid environment while other cells like the ciliated trachea of man use the coordinated beating of many cilia to propel a liquid or mucous environment over the surface of the ..

..We will focus on two epithelia, the epididymis, which is at the core of our research program, and the trachea. The epididymis, which connects the testis to the vas deferens, is involved in the maturation and storage of ..

..Acquired tracheomalacia, a condition defined by excessive expiratory collapse of the trachea due to weakness of the airway walls and/or supporting cartilage, is a relatively common but frequently overlooked ..

..experiments, we have generated more than 30 monoclonal antibodies specific to ciliated cells of the trachea. Some of these monoclonal antibodies reveal subpopulations of ciliated cells not apparent from histology alone...

..hypotheses regarding mechanisms of the activation of responses and the directed migration of hemocytes and trachea. Cell-specific expression of RNAi and wild type alleles of proposed components of the responses will dissect ..

..of this proposal is to elucidate the role of multiple inositol polyphosphate phosphatase (Mipp1) in Drosophila trachea development, which is one of the best model systems for studying tubular organogenesis...

..First apparent in the distal lung buds, the cells spread upward throughout the bronchi and trachea, and eventually formed separate cell populations in the tracheo- bronchial and bronchioloalveolar regions...

..using a multi-electrode array to obtain multiple-spike train data during: a) the selective stimulation of the trachea or pharynx to elicit cough or swallow;b) simultaneous stimulation of the trachea and pharynx to elicit ..

..Modification of the material and design of the ETT cuff that is positioned in the trachea, and/or continuous aspiration of subglottic secretions through an extra port positioned between the tube cuff and ..

..reduce this damage by synchronizing the ventilator so that it can be used non-invasively without a tube in the trachea. The new generation of ventilators will also reduce the amount of effort needed to save the premature infant and ..

..pheasants raised on propagation farms can be routinely infected with Heterakis gallinae (cecal worm), Syngamus trachea (gapeworm) and other parasitic worms that are susceptible to fenbendazole treatment...

..class of inflammatory mediators are the leukotrienes C4 and D4 (LTC4 and LTD4) which when applied to gut, trachea, and pure cultures of sensory neurons elicit release of the tachykinins substance P (SP) and substance K (SK)...

..by mucus secretions which are synthesized in the surface epithelium and in submucosal acinotubular glands in the trachea. The surface epithelium of the tracheobronchial airway is coated with mucus which is constantly removed by the ..