Extensive research has been conducted globally into conceptual frameworks for ecosystem services, the most notable being the Millennium Ecosystem Assessment (Millennium ecosystem assessment: ecosystems and human well-being; a framework for assessment. World Resources Institute, Washington, DC, pp 51, 53-55, 2005). The South East Queensland (SEQ) Ecosystem Services Framework (Australia) aims to provide the tools to enable government, industry, business, researchers, non-government organizations and land managers to apply the concept of ecosystem services in their planning and management practices. This article describes the Framework and the process that has produced matrices and maps that identify and illustrate the linkages between ecosystems, ecosystem functions, ecosystem services and the communitys well-being. The matrices and maps derived can identify areas in the region where the most ecosystem services are generated. This allows areas to be considered as valuable natural assets of the region, deserving appropriate protection measures or significant offsets if they are diminished or degraded in any way. Although the Framework requires further refinement and ongoing development, the process applied and the products produced has enabled decision makers to turn the concept of ecosystem services into practical application in SEQ.