Earlier this week I posted a query about where to get anti-mouse antibodies
with low "background" reactivity to worm proteins. Many respondents mentioned
being satisfied with antibodies from Jackson Immunoresearch. No other supplier
was mentioned frequently.
Many also suggested pre-adsorption against worm acetone powder. This is
certainly reasonable and I've done it in the distant past, but I resent the
suppliers forcing me to perform an extra step when I'm already paying them for
"affinity purified" antibodies. I guess the lesson is: affinity purification is
no guarantee.
Thank you all for your help.
Lew Jacobson
Dept. Biol. Sci.
Univ. of Pittsburgh
LJAC at vms.cis.pitt.edu