The Wnt family of secreted glycoproteins mediate cell-cell interaction during cell growth and differentiation in both embryos and adults. (1,2) Canonical Wnt signaling by way of the b-catenin pathway is transduced by two receptor families, frizzled proteins and lipoprotein-receptor-related proteins 5 and 6 (LRP5/6) which binds Wnts and transmit their signal by stabilizing intracellular b-catenin.(3-6) Kremen1 and Kremen2 are high-affinity Dkk1 receptors that functionally cooperate with the secreted protein Dickkopf1 (Dkk1) to block Wnt/b-catenin signaling.(7) Kremen1 and Kremen2 are components of a membrane complex modulating canonical Wnt signaling through LRP6 in vertebrates.(7) Dkk1 has been shown to inhibit Wnt signaling by binding to and antagonizing LRP5/6 (8-10).

Catalog #

K5002

Applications

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution

Western Blot: 0.1-2ug/ml

Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Clone Type

Polyclonal

Isotype

IgG

Host

Rabbit

Source

Human

Concentration

~0.125mg/ml

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.