Mempro™ Cell-Free Ion Channel Superfamily Production

Based on the comprehensive membrane protein production platform established through years of experience, scientists from Creative Biostructure is able to provide you best custom Mempro™ cell-free ion channel superfamily protein production services. Our patent cell-free expression platform can bypass the limitations that encountered in cell-based protein production system, such as host cell toxicity, low yield and solubilization and purification using detergents.

Ion channel is a type of pore-forming transmembrane protein that has a wide range of biological functions, such as regulating cell volume, setting up a resting membrane potential, forming action potentials and other electrical signals by gating the flow of ions across the cell membrane, controlling the flow of ions across secretory and epithelial cells, etc. Ion channels exist in the membranes of all cells, which are considered to be one of the two traditional classes of ionophoric proteins, with the other class known as ion transporters, including the sodium-potassium pump, sodium-calcium exchanger, and sodium-glucose transport proteins, and amongst others. It is reported that the open-state of an ion channel facilitates the movement of ions into or out of the cell in single-file manner. In addition, individual ion channels are specific to particular ions, meaning that they usually allow only a single type of ion to pass through them.

• Mempro™ Ion Channel Superfamily Production in Rabbit Reticulocyte
Crude rabbit reticulocyte lysate is the excellent system for eukaryotic membrane protein production, providing multiple options for the optimization of the production protocols, such as the availability of materials, protein origin and complexity, downstream processing, etc.

• Mempro™ Ion Channel SuperfamilyProduction in Baculovirus
Baculovirus expression system from Autographa californica nucleopolyhedrovirus (AcNPV) is also a powerful system for transmembrane protein expression, which is developed in the early stage that transfected insect cells in combination with vectors derived from the baculovirus.