Tuesday, December 4, 2007

Optimized ChIP Protocols

The optimization resulted in two protocols that both have more than 10-fold higher throughput than the original. One of the protocols was optimized for speed and requires only 1.5 days to complete with a 46% average improvement in signal-to-noise ratio over the original protocol. The second protocol, optimized for signal-to-noise, requires 2.5 days to complete and achieves a 293% average improvement in signal-to-noise ratio over the original protocol. The optimizations were done in E. coli using three different transcription factors (two were primarily for validation).Download the new ChIP Protocols (pdf)