The aim of digital subtraction of radiographic images is eliminating the additional shades to increase the resolution and quality of the images. In dentistry, digital subtraction technique can be used for diagnosis of progressive periodontal diseases or evaluation of the amount of jaw bone repair or destruction. In this study, the possibility of digital subtraction with non-expensive techniques by the use of a personal computer has been investigated. To do so, first we prepared initial we digitalized the obtained radiographs by the use of a scanner. Then we tested the subtraction on two available scanner-specific soft wares which had the ability to digitally subtract the images. The results have been discussed in the full text of the article.