CMV R-GENE® advantages

Cytomegalovirus (CMV) is a serious complication for immunocompromised patients. That’s why you want optimized detection and monitoring of CMV infection. CMV-R-GENE® is an ideal solution, offering rapid and specific detection. You can detect infection prior to clinical symptoms, improving options for management; test patients during treatment to measure the effectiveness of treatment; and test after treatment to monitor for relapse. Plus – for added efficiency – using the broad R-GENE® range lets you qualify and/or quantify various viruses in one sample or analyze various samples for one virus at the same time.

Protocol to convert quantification blood sample results into IU/mL with the WHO 1st International Standard

Flexible

Validated for use with various samples types

Use manual or automated sample preparation such as NucliSENS® easyMAG® and assay setup platform such as easySTREAM™ liquid handling system

Qualified with the major real-time PCR platforms

Everything you need in one kit

The CMV R-GENE® kit is a ready-to-use molecular detection kit. It detects and measures the CMV genome using real-time PCR after viral DNA extraction. It works by amplifying and simultaneously detecting a specific region of the CMV genome using 5’ nuclease Taqman technology.

An Internal Control (IC2) checks the extraction process, including lysis, and the presence of amplification inhibitors in the sample

Includes necessary reagents optimized to detect and quantify CMV for in vitro diagnostic use

Easy procedure

Using the CMV R-GENE® kit is simple. Just add the sample extracted DNA to the ready-to-use PCR master mix and start the reaction on the appropriate Real-Time PCR thermocycler, following optimized cycling program described in the “Instructions For Use”.

Fast facts on CMV

What is CMV?

The human cytomegalovirus (HCMV) is a double-stranded enveloped DNA virus of the Herpesviridae family. After primary infection, which often occurs in young childhood, HCMV remains in its latent state in the host. While healthy people with CMV are usually asymptomatic, HCMV may cause recurrent secondary infections, during chronic or transient immunosuppression.

Who is most at risk?

HCMV infection is the most common post-transplant infectious agent after organ transplant or bone marrow allograft. Prolonged fever may be the only clinical manifestation. However, it may comprise a number of complications including interstitial pneumonitis, a major complication that occurs in about 20% of all graft recipients and has a 90% mortality rate without treatment. Infection with HCMV aggravates the immunosuppression, favours superinfections and is a factor that triggers or accelerates rejection or GVH (reaction of the graft against the host).

While the incidence of infections with HCMV during HIV/AIDS has decreased by 80% since the use of highly active anti-retroviral treatments, testing is still important to ensure control of CMV and help prevent related opportunistic infections. Clinical manifestations for HIV/AIDS patients, most commonly retinitis and peptic ulcers, occur when immunosuppression is high (CD4+ T lymphocytes under 50/mm3).

During pregnancy, the advent of a maternal primary infection gives rise to complications in 50% of the cases of foetal infection, with this being severe in approximately 10% of cases, with neurological impairment occurring in particular.

What are the benefits of CMV testing?

Real-Time PCR-based assays for CMV enable rapid and specific detection prior to clinical symptoms to help improve outcomes, especially important for organ transplant/bone marrow allograft and HIV/AIDS patients. Testing helps keep track of the effectiveness of active treatment and can monitor for relapse after treatment.

CMV R-GENE® and the 1st WHO International Standard for Human Cytomegalovirus