Data extracted from this reference:

Cloned(Commentary)

Commentary

Organism

-

Agrobacterium tumefaciens

Crystallization (Commentary)

Crystallization

Organism

crystal structures of an apo-form, a ternary form in complex with NADH and product, and an inactive Y136A mutant in complex with NAD+. Enzyme is a homohexamer. The monomer contains a Rossmann fold, essential for nucleotide binding. The ternary complex structure reveals a product, D-galactaro-1,5-lactone, which is bound above the nicotinamide ring. This product rearranges in solution to D-galactaro-1,4-lactone

Agrobacterium tumefaciens

Engineering

Amino acid exchange

Commentary

Organism

Y136A

inactive. Crystal strucuture shows changes in the position of residues Ile74 and Ser75. This probably alters the binding of the nicotinamide end of NAD+

Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

D-galacturonate + NAD+ + H2O

Agrobacterium tumefaciens

-

D-galactarate + NADH + H+

-

-

?

D-galacturonate + NAD+ + H2O

Agrobacterium tumefaciens C58

-

D-galactarate + NADH + H+

-

-

?

D-glucuronate + NAD+ + H2O

Agrobacterium tumefaciens

-

D-glucaric acid + NADH + H+

-

-

?

D-glucuronate + NAD+ + H2O

Agrobacterium tumefaciens C58

-

D-glucaric acid + NADH + H+

-

-

?

Organism

Organism

Primary Accession No. (UniProt)

Commentary

Textmining

Agrobacterium tumefaciens

Q7CRQ0

-

-

Agrobacterium tumefaciens C58

Q7CRQ0

-

-

Substrates and Products (Substrate)

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

D-galacturonate + NAD+ + H2O

-

725435

Agrobacterium tumefaciens

D-galactarate + NADH + H+

-

-

-

?

D-galacturonate + NAD+ + H2O

-

725435

Agrobacterium tumefaciens C58

D-galactarate + NADH + H+

-

-

-

?

D-glucuronate + NAD+ + H2O

-

725435

Agrobacterium tumefaciens

D-glucaric acid + NADH + H+

-

-

-

?

D-glucuronate + NAD+ + H2O

-

725435

Agrobacterium tumefaciens C58

D-glucaric acid + NADH + H+

-

-

-

?

Cofactor

Cofactor

Commentary

Organism

Structure

NAD+

-

Agrobacterium tumefaciens

Cloned(Commentary) (protein specific)

Commentary

Organism

-

Agrobacterium tumefaciens

Cofactor (protein specific)

Cofactor

Commentary

Organism

Structure

NAD+

-

Agrobacterium tumefaciens

Crystallization (Commentary) (protein specific)

Crystallization

Organism

crystal structures of an apo-form, a ternary form in complex with NADH and product, and an inactive Y136A mutant in complex with NAD+. Enzyme is a homohexamer. The monomer contains a Rossmann fold, essential for nucleotide binding. The ternary complex structure reveals a product, D-galactaro-1,5-lactone, which is bound above the nicotinamide ring. This product rearranges in solution to D-galactaro-1,4-lactone

Agrobacterium tumefaciens

Engineering (protein specific)

Amino acid exchange

Commentary

Organism

Y136A

inactive. Crystal strucuture shows changes in the position of residues Ile74 and Ser75. This probably alters the binding of the nicotinamide end of NAD+