Abgent is a leading provider of Custom Peptide Synthesis. Our services are driven by a personalized approach toward your needs. With fifteen years of experience, our experienced team employs the latest in instrumentation and specialized synthetic techniques to deliver high quality products on time at a great price.

Abgent has over fifteen years of experience producing recombinant proteins in E. coli and mammalian cells (CHO and HEK293, etc), and we have added a powerful yeast expression platform to our menu of services. With state-of–the art molecular biology and protein biochemistry labs, we work with our clients to rapidly evaluate in parallel to identify the optimal expression system for candidate proteins.

Certify that your antibodies work, while saving time and money. Abgent’s experienced staff custom validates more than 1,000 antibodies each month in Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC) and additional applications. Proper validation with controls and optimization ensure the quality of the antibody, and establish confidence in your results.

Thousands of laboratories across the world have published research that depended on the performance of biological reagents manufactured by Abgent to advance their research. Check out links to articles that cite our custom service antibodies, peptides, and proteins in major peer-reviewed journals, organized by research category.

18,000+ products cover the ten top research categories, including cancer, metabolism, cardiovascular, neuroscience, and stem cells. Each antibody is crafted with care according to rigorous protocols for immunogen design and preparation, presentation to host animal, and high-affinity purification against the antigen. Quality is embedded in the production process to assure the best antibody for you.

Abgent develops 1000+ new products per year. We provide tools for path-breaking research by developing antibodies that detect a comprehensive library of novel and established targets. For established targets we seek to add antibodies that recognize new epitopes, including post-translational modifications such as phosphorylation and methylation. For new targets we consult with leading experts to accelerate development of antibodies that will propel state-of-the-art research in cellular health and disease.

Abgent offers custom and catalog peptides to pharmaceutical, biotechnology companies and academic institutions worldwide. All peptides are manufactured in the United States to exacting standards in support of U.S. and international clientele. Custom peptide services include long peptides (>100mer), cyclic peptides, difficult sequences, fluorescent labels, and phosphopeptides.

The majority of Abgent antibodies are produced using peptide immunogens. These immunospecific peptides can be used as blocking agents when using the complementary antibodies in a range of applications.

Researchers worldwide depend on Abgent FLcDNA clones to accelerate their research. Expression-ready clones are convenient solution that decreases the time required to start an experiment and increases the chances of success.

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Abgent, a WuXi AppTec company, is a leading manufacturer of primary antibodies with more than fifteen years of experience. We supply quality antibodies and related products drug discovery and academic laboratories worldwide. Our focus is on targets covering the major research areas impacting health and disease, including cancer, metabolism, cardiovascular, neuroscience, and stem cells.

Founded in 2001, Abgent is one of the world's largest manufacturers of research user only antibodies. WuXi Apptec, a leading global contract R&D services provider serving the pharmaceutical, biotech, and medical device industries, acquired Abgent in October of 2011.

With its history as an original manufacturer, Abgent has a deep and practical understanding of the production process for antibodies, peptides, and recombinant proteins. We have assembled world-class, independently credentialed facilities, operate under a strict project management paradigm with highly trained staff, rigorously test our products in-process and post-production, and monitor customer feedback to assure that we earn the trust of scientists who choose to work with us.

Abgent may in-licenses select products and technologies to offer our customers a wide variety of options for their proteomic research needs. Contact us for further information and non-disclosure agreements. Our experienced discovery and production teams will work with you to jointly evaluate proposals and identify partnering opportunities.

We believe that attracting and supporting creative and talented people is essential to our success. Abgent offers an energetic, friendly environment and the opportunity to further scientific research and drug discovery. Abgent is an Equal Opportunity Employer. Send a cover letter and resume to jobs@abgent.com

The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.

The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.

TGIF1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of lysate from A549 cell line, using TGIF1 Antibody (N-term)(Cat. #AP14571a). AP14571a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

This TGIF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-109 amino acids from the N-terminal region of human TGIF1.

Dilution

WB~~1:1000

Format

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Precautions

TGIF1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information

Name

TGIF1

Synonyms

TGIF

Function

Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII- RXRE). Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Active transcriptional corepressor of SMAD2. Links the nodal signaling pathway to the bifurcation of the forebrain and the establishment of ventral midline structures. May participate in the transmission of nuclear signals during development and in the adult, as illustrated by the down-modulation of the RXR alpha activities.

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Background

The protein encoded by this gene is a member of the
three-amino acid loop extension (TALE) superclass of atypical
homeodomains. TALE homeobox proteins are highly conserved
transcription regulators. This particular homeodomain binds to a
previously characterized retinoid X receptor responsive element
from the cellular retinol-binding protein II promoter. In addition
to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha
transcription activation of the retinoic acid responsive element,
the protein is an active transcriptional co-repressor of SMAD2 and
may participate in the transmission of nuclear signals during
development and in the adult. Mutations in this gene are associated
with holoprosencephaly type 4, which is a structural anomaly of the
brain. Alternative splicing has been observed at this locus and
eight variants, encoding four distinct isoforms, are described.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abgent.com.