The pGLO™ Kit SDS-PAGE Extension is designed to be performed following the pGLO Bacterial Transformation Kit. The extension is designed to identify and visualize green fluorescent protein (GFP) from the proteome of transformed E. coli, which also contains thousands of other proteins. Because only the cloned GFP glows, students can use this phenotypic trait to quickly identify the GFP within the complex mix of proteins.

Features and Benefits

With this kit, students are able to:

Perform real biotechnology workflows

Identify proteins by conformation and size

Link gene induction to protein expression to protein identification

Understand chromophores and the basis of protein fluorescence

Extend the pGLO Bacterial Transformation Kit

How It Works

In the first part of the exercise, students use the pGLO Bacterial Transformation Kit to transfer pGLO plasmid encoding GFP into E. coli, a common prokaryotic organism used for DNA propagation and protein expression. Colonies of E. coli are qualitatively examined for fluorescence to determine whether the pGLO gene is being expressed. In the second part of the laboratory activity, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is employed to separate the entire repertoire of proteins expressed in E. coli, including the foreign GFP responsible for transferring the fluorescence trait.

More Information

Three-session laboratory activity, 45 min per session

Provides sufficient materials for eight student workstations, up to four students per workstation.

For more than 15 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows high school and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices.

How do I apply for the education discount?

If you are an educator at the high school or college level, visit our Education Discount Policy page to establish an education account number.

If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the list price. If the problem persists, please call customer service at
or send an email to