製品の特性

製品の状態Liquid

保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Concentration information loading...

特記事項（標識）HRP or AP conjugated to a polymer backbone, which is collectively conjugated to the antibody.
The end result is two antibody subsets:
1) Multiple HRP molecules linked to goat anti-rabbit antibodies.
2) Multiple AP molecules linked to goat anti-mouse antibodies.

ポリ/モノポリクローナル

アイソタイプIgG

特記事項

This product is specially designed for a rapid double stain procedure. The overall staining procedure can be done in 4 steps and in less than two hours. Avidin-biotin blocking procedures are not necessary when using this product. This new and innovative AP and HRP polymerization technology provides a significant increase in staining sensitivity when compared to other conventional AP or HRP conjugated secondary antibodies.

This product is provided in a pre-diluted, ready-to-use format. Procedure (adapt in-house protocols to accommodate): 1) A primary antibody cocktail with a rabbit monoclonal/polyclonal and a mouse monoclonal/polyclonal antibody must be applied for the standard duration. Rinse in TBS. (DO NOT USE PBS). 2) Apply ab2892 for 20 - 30 minutes then rinse in TBS (DO NOT USE PBS). 3) Chromogen 1: Develop AP using suitable chromogen. Rinse in TBS. 4) Chromogen 2: Develop HRP using suitable chromogen. Rinse in TBS. Equally suited to both manual and automated immunostaining proceedures. This product has not been tested in any application requiring further dilution of the antibody and therefore it is not recommended for use in such applications.

Thank you for your enquiry.
Yes, this can be used to perform single staining. However, I would recommend that your customer uses an HRP or AP conjugated secondary antibody together with a suitable chromogen as this would be a more simplistic approa...

Thank you for your enquiry and your interest in our products.
We normally suggest TBS instead of PBS for IHC staining. For details on IHC and antigen retrieval, please click on the Resources section on the top panel of the Abcam homepage, you might...

Sorry but we do not have a protocol for double staining although we do plan to create a general double staining protocol in the next few months. Please keep an eye on the protocol section on the Abcam website for details.

asdf EdoAbcam Scientific Support

Answered on Oct 13 2003

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"