Proteasome Activity Assay

ZsProSensor-1 is a proteasome-sensitive fluorescent reporter. It is a fusion of a bright green fluorescent protein (Exmax = 496 nm, Emmax = 506 nm) with a degradation domain* which targets the protein for rapid degradation by the proteasome. The cells emit green fluorescence when there is a drop in proteasome activity:

Green fluorescence accumulates – the proteasome is inhibited

No accumulation of green fluorescence – the proteasome is functioning normally in the cell

The Proteasome Sensor Vector uses the CMV immediate early promoter to drive high levels of ZsProSensor-1 expression.

Features

Applications

Drug screens

Cancer studies

Image based HTS/HCS

Real-time proteasome studies

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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