Advantages of using the Antigen-Antibody Pens-Using a proprietary and patented technology, ADI has designed and developed specialized fountain pens, called Antigen-Antibody pens-TM, that will allow researchers to deliver or write/mark the blotting membranes in any form or shape or size. A specially formulated antigen-bonded colored dyes helps to see antigen being written on the membrane. One can mark the size of the markers or the lanes. The blots are processed as usual with the appropriate 2-ab antibody conjugates, and ECL: results recorded on films or digital imager. Unlike the situation in Fig.1C, the use of antigen pens allows the markings to be visible at the end. Therefore, it is possible to see right away, what samples produced results and what the sizes are even if the whole blot image size were to change. There is no need to compare with the original. In addition, Antigen-Antibody Pens are formulated to react only with 2-ab-enzyme conjugates from a give animal species. Therefore, if one is using rabbit primary antibody and the antigen pens specific for rabbit, then the secondary antibody must be anti-rabbit for the Antigen-antibody pens writing to be visible. Any error in the use of wrong conjugate or substrate will produce no writing (control failed) and, of course, the entire blot will be negated (no protein bands in the samples). Therefore, usage of antigen pens provides a built-in positive control for the entire Western procedure. Antigen-Antibody pens are available for various antibody host species.

In addition, the Antigen Pens are also formulated with Color-coded dyes, example Red dye for Rabbit, Green dye for Goat, and Black dye for Mouse primary antibodies. Therefore, there will be an added visual control regarding the usage of various Antigen pens. The body of the pen is matched with the antigen dye as well. For example, Red pen for Rabbit, Green Pen for Goat, and Black pen for Mouse.

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