Abstract

Background

Fluorescence-based biological imaging has been revolutionized by the recent introduction
of superresolution microscopy methods. 3D superresolution microscopy, however, remains
a challenge as its implementation by existing superresolution methods is non-trivial.

Methods

Here we demonstrate a facile and straightforward 3D superresolution imaging and sectioning
of the cytoskeletal network of a fixed cell using superresolution optical fluctuation
imaging (SOFI) performed on a conventional lamp-based widefield microscope.