Strain Name:

B6.129S7-Chrna7tm1Bay/J

Stock Number:

003232

Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use

These alpha7 nicotinic acetylcholine receptor knockout mice may be useful in studying the cholinergic anti-inflammatory pathway; including inflammatory neurotoxicity in stroke, myocardial infarction, sepsis and Alzheimer's disease, as well as neuropsychiatric diseases such as schizophrenia.

DevelopmentA null mutation of the alpha7 subunit was prepared by deleting the last three exons (8-10) of the Chrna7 gene. Mice deficient in the alpha 7 subunit were generated by introducing a 7 kb deletion into ES cells. The strain originated on a mixed 129/SvEv and C57BL/6 background. The donating investigator backcrossed these mice to C57BL/6 for approximately eight generations ("N8F8") prior to sending to The Jackson Laboratory.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 1 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed genetic background.

Chrna7tm1Bay/Chrna7+

Chrna7tm1Bay/Chrna7tm1Bay

B6.129S7-Chrna7tm1Bay/J

reproductive system phenotype

*normal* reproductive system phenotype

female homozygotes have normal ovaries and enter puberty at the expected age, with no significant differences in the onset of vaginal opening or cornification relative to wild-type or heterozygous control females (MGI Ref ID J:92540)

many nonmotile sperm exhibit a quivering head and midpiece but no forward movement, confirming that cells are alive (MGI Ref ID J:115434)

however, no significant differences in sperm viability, senescence rate or spontaneous acrosome reaction rate are observed between mutant and wild-type sperm before and after capacitation (MGI Ref ID J:115434)

decreased litter size

litters born to homozygous mutant females are significantly smaller than those born to heterozygous females regardless of the male genotype (MGI Ref ID J:92540)

the extent of asynchrony is variable within the mutant female population, but for a given animal, the average cycle length tends to remain stable at young mature ages (4-6 months) and middle ages (10-14 months) (MGI Ref ID J:92540)

all female homozygotes eventually complete an estrous cycle, and may achieve pregnancy but with a smaller number of surviving pups (MGI Ref ID J:92540)

reduced female fertility

the number of live births is significantly less from matings of female homozygotes with either heterozygous (4.5 ¿ 2.12) or homozygous (3.7 ¿ 0.53) mutant males in comparison to matings of heterozygous females and males (7.34 ¿ 0.04) and matings of heterozygous females and homozygous mutant males (7.1 ¿ 0.40) (MGI Ref ID J:92540)

nervous system phenotype

abnormal GABA-mediated receptor currents

cultured cortical pyramidal neurons show a reduction in sIPSC frequency and current decay time, but not amplitude, indicating reduced GABAergic neurotransmission (MGI Ref ID J:214099)

abnormal GABAergic neuron morphology

levels of markers of GABAergic maturation are reduced in the neocortex indicating that cortical parvalbumin GABAergic development is impaired (MGI Ref ID J:214099)

in the delayed matching-to-place task of the Morris water maze, mutants take longer to find the hidden platform than controls, indicating a minor impairment in working/episodic memory (MGI Ref ID J:123663)

however, mice show normal latency to enter the central zone of the open field, normal elevated plus maze behavior, and no differences in swim speed or thigmotaxic behavior (MGI Ref ID J:123663)

neither cholinergic compensatory mechanisms nor alterations in binding levels or affinity for nicotinic ligands, such as epibatidine or nicotine, can account for the retained sensitivity to the convulsant effects of nicotine (MGI Ref ID J:74703)

abnormal spatial learning

homozygotes locate the hidden platform in the Morris water task significantly faster than wild-type; however, this is a subtle difference and is not supported by the distance traveled data (MGI Ref ID J:51149)

neither cholinergic compensatory mechanisms nor alterations in binding levels or affinity for nicotinic ligands, such as epibatidine or nicotine, can account for the retained sensitivity to the convulsant effects of nicotine (MGI Ref ID J:74703)

Chrna7tm1Bay/Chrna7tm1Bay

involves: 129S7/SvEvBrd * C57BL/6

respiratory system phenotype

abnormal airway basal cell differentiation

after polidocanol-induced acute injury, 8-wk old mutant mice display delayed regeneration of the tracheal epithelium with a transient hyperplasia of basal cells observed on days 4 and 6, but not on day 8, after injury (MGI Ref ID J:154705)

airway basal cell hyperplasia

at 1 year of age, the % of 1-2 layers of basal cells in the tracheal epithelium is reduced whereas the % of 3-4 layers of basal cells in increased by 3.8-fold relative to that in wild-type controls (MGI Ref ID J:154705)

cellular phenotype

abnormal airway basal cell differentiation

after polidocanol-induced acute injury, 8-wk old mutant mice display delayed regeneration of the tracheal epithelium with a transient hyperplasia of basal cells observed on days 4 and 6, but not on day 8, after injury (MGI Ref ID J:154705)

A 7 kb genomic fragment containing exons 8-10 was replaced with a neomycin selection cassette. The deleted sequences encode the second through the fourth transmembrane domains and the cytoplasmic loop. Northern blot analysis on brain samples derived from homozygous mice demonstrated that no detectable transcript was produced from this allele. Western blot analysis on brain extracts derived from homozygous mice also confirmed that no detectable protein was made from this allele. [MGI Ref ID J:122939]
[MGI Ref ID J:44288]

Health & Colony Maintenance Information

Animal Health Reports

Colony Maintenance

Breeding & Husbandry

When maintaining a live colony, homozygous mice may be bred to heterozygous mice. Although homozygotes are viable and fertile, the donating investigator reports that breeding is better using heterozygous breeders, and they have observed reduced fertility in homozygotes. Heterozygotes produce small litters. Expected coat color from breeding is Black.

Standard Supply

Repository-Live.Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations

Live Mice

Price per mouse (US dollars $)

Gender

Genotypes Provided

Individual Mouse

$301.60

Female or Male

Heterozygous for Chrna7tm1Bay

$301.60

Female or Male

Homozygous for Chrna7tm1Bay

Price per Pair (US dollars $)

Pair Genotype

$603.20

Heterozygous for Chrna7tm1Bay x Homozygous for Chrna7tm1Bay

$603.20

Homozygous for Chrna7tm1Bay x Heterozygous for Chrna7tm1Bay

Standard Supply

Repository-Live.Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Standard Supply

Repository-Live.Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering InformationJAX® MiceSurgical and Preconditioning ServicesJAX® ServicesCustomer Services and Support
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