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Before certain experiments, it is useful to take a series of measurements while key acquisition parameters change. The resultant data can then be utilized to optimize acquisition conditions. There are several situations involving temperature in which this approach may be beneficial. For example, the researcher might want to characterize the dark current of a camera so as to monitor its performance. Another situation involving temperature is when you want to ensure the best signal-to-noise ratio for an experiment whose SNR is affected by an inherent tradeoff between quantum efficiency and dark current, as is the case when working with particular wavelength ranges in the near-infrared region.

LightField - How do I normalize my data? [+][-]

This formula scales the data so the highest peak is normalized to one.

This can be applied during acquisition under Online Processes, or in post processing under Processes - Formula

IMPORTANT: Do not use normalization during live acquisition when running step and glue because it will normalize every frame as acquired resulting in mismatches between the spectral data when it is stitched together.

LightField - Calculate Average Pixel Intensity in Real Time [+][-]

This formula courtesy of Dr. Sebastian Remi, PI’s Northeast Sales Engineer, displays the average pixel intensity of an ROI in real time during Run or Acquire. You can also select F6 to display statistics for the file or ROI. This will update during live acquisition, however only at a rate of approximately one update every 1-2 seconds.

Note: The numeric input “53:293,47:186” shows the ROI for the formula. Instead of typing in the numbers that define the ROI, you can use the input selection wizard in the formula editor. It looks like this:

When you select Run or Acquire, average intensity is displayed live however you will not see a live image.

LightField - Formulas: Set threshold for an image and multiply by the orginal input [+][-]

This formula sets a threshold for an image and then multiplies this result by the orginal input (image intensity data). All the data above the threshold remain unchanged while data below the threshold is set to a value of zero (0).

Note: The formula example above includes a threhold of 1000 counts. You should change this value to correspond to the intensity dtaa in your specific experiment.

Original image:

Formula:

Original image (top) vs. Image after LightField Math (bottom)

Note: Both images above were scaled to show background intensity. Peak intensities are equal for the original and modified images, while the background in the bottom image has been completely subracted.

LightField - is not displaying individual frames [+][-]

When averaging or summing multiple frames, LightField does not display individual frames as they are collected. Instead, LightField displays the “waiting for first live frame” message.