(none)

In article <9103140043.AA14252 at sdchemd1.ucsd.edu> mwsmith at UCSD.EDU (Mike Smith) writes:
>> Has anyone tried the Strataclean resin from Stratagene? Today I used
I haven't tried it, but to answer your next question...
>and sequencing. The miniprep DNA did cut with SstI, but I wonder if the
>background RNA will interfere with sequencing.
I do all my DNA sequencing from phenol/CHCl3 extracted miniprep dsDNA, with
the E.coli RNA still there. The presence of RNA doesn't appear to matter,
as I can usually read ~400 bases in a given reaction. However, I would like
to switch to something like Strataclean myself.
===============================================================================
Brian Fristensky |
Department of Plant Science |
University of Manitoba |
Winnipeg, MB R3T 2N2 CANADA | THIS is THIS!
frist at ccu.umanitoba.ca |
Office phone: 204-474-6085 |
FAX: 204-275-5128 |
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