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The present study was carried out to investigate the seasonal variations in physico chemical and biological parameters of River Indus for a period of eleven months from November 1993 to September 1994. For this purpose five different locations, Kaloorkot, Darya Khan, Ghazi Ghat Taunsa Barrage and Chachran Sharif were selected for sampling. Water samples were collected on monthly basis and were analyzed for estimation of water temperature, temperature above surface of water, temperature above surface of bank, pH and total dissolved solids. Only Darya Khan was selected for studying planktonic life. The minimum value of temperature of water 0°C in winter months while the maximum temperature of water was 36.67°C in the month of July. The minimum pH 6.9 was recorded in the month of June, while in winter months pH goes on increasing, reaching up to 8.9 showing that in winter months pH of water increases and in summer months pH of water decreases. The minimum value of total dissolved solids was 0.3 mg l-1 in April and 12.49 in August showing an increase in total dissolved solids in winter months. During the whole study 43 different genera of phytoplankton and 17 different genera of zooplankton were observed at Darya Khan. The parameters were analyzed to investigate probable pollution at different points in River Indus to suggest ways and means to improve the feeding and conservation of Indus Dolphin ecology.

The current study was started from March to December 2001 to investigate the seasonal variations in biological parameters and biodiversity in water of River Indus at Ghazi Ghat (D.G. Khan) by analyzing the frequency of occurrence, relative abundance and diversity index of planktonic life. Density and diversity of Planktonic life was used as a measure of water quality. Phytoplankton were abundant as compared to Zooplankton. 125 Phytoplankton genera were recorded. Among these 17 of Cyanophyta, 3 of Cryptophyta, 1 of Pyrrophyta, 10 of Chrysophyta, 5 of Xanthophyta, 18 of Bacillariophyta, 8 of Euglenophyta, 49 of Chlorophyta, 4 of Charophyta, 1 of Rhodophyta, 7 of Macrophyta and one genera of filamentous algae. 44 genera of Zooplankton were observed including 22 of Protozoans, 10 of Rotifers, 6 of Cladocera and 6 of Copepoda.