For selecting colonies, the appropriate dose must be determined empirically for each specific cell line. We recommend to test a dosage range using dishes of untransfected cells and choose the dose that kills all of the cells in 3–5 days. If all the cells die in less than 24 hr, you should use a lower dose.

Puromycin dihydrochloride: A broad spectrum antibiotic that inhibits protein synthesis in both prokaryotic and eukaryotic organisms.

Properties

G418 Sulfate

Molecular Weight: 692.7

Chemical Formula: C20H40N4O10·2H2SO4

Form: White to Off-White Powder

Puromycin

Molecular Weight: 544.4

Chemical Formula: C22H29N2O5·2HCl

Form: White Powder

Hygromycin B

Molecular Weight: 527.5

Chemical Formula: C20H37N3O13

Form: Sterile Brown Solution at 50 mg/ml in PBS

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.