rna from plasmid

i am still new in this field and i would like to ask from which link or reference materials can i know the procedure and concept of synthesizing biotinylated rna from plasmid with the gene of interest into insert to the plasmid.

The plasmid you use must have a T7 or SP6 or T3 RNA polymerase recognition site. So you can use one of these RNA polymerase to synthesis the RNA of your interested gene in vitro. Using the Biotin-labled UTP instead of UTP in the transcription reaction.A lot of companies have kit for the in vitro transcription of biotinated RNA. The url listed below is just obtained by a google search.https://www.roche-ap...f/ISH_54-57.pdfhttp://www.epibio.com/item.asp?ID=519