Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Applications

Specificity and Use

Recognizes human p300 and CBP. p300 and CBP differ by three noncontiguous residues within the 21aa epitope region. A difference that does not detectably affect the reactivity of LS-C12614. Species cross-reactivity: monkey, mink, mouse and rat.

Usage:

Suitable for use in Immunofluorescence and Immunohistochemistry. Not suitable for use in Western Blot. Immunohistochemistry (Formalin/paraffin): 1:50-1:100 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Tonsil. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Description: CREBBP / CREB Binding Protein is ubiquitously expressed and is involved in the transcriptional coactivation of many different transcription factors. First isolated as a nuclear protein that binds to cAMP-response element binding protein (CREB), this gene is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition.