Actobacter xylinum has been subcultured repeatedly to prepare N-acetylglucosamin (GlcNAc) residue induced bacterial cellulose (2-4 mole%) applying GlcNAc containing glucose medium. As a similar level of GlcNAc residue incorporation has been made by applying glucosamin or galactosamin as carbonsource instead of GlcNAc, but mannosamin was not effective, participation of deaminase was suggested on the GlcNAc residue incorporation. Since ammonium ion was assumed to be final product by deaminase, gulucose and ammonium salts were applied successfully to incorporation of GlcNAc into bacterial cellulose by applying a new type of aerobic rotatory cultivator. A shallow pan cultivator has been made to prepare fibrous novel bacterial celluloses successfully. These fibers were shown to have higher tensile strength than that of cotton.