Methods :
A prospective study was conducted in a tertiary hospital in São Paulo, Brazil. From October 2015 to November 2016, patients with clinical suspicion of endophthalmitis had vitreous humor samples inoculated in blood culture bottles (BCBs). Positive BCBs were initially seeded in culture plates according to standard procedures. After standard procedure, aliquots from positive BCBs were immediately submitted to a simple in-house protein extraction protocol for direct ID by MALDI-TOF MS. The microorganisms’ ID and turnaround time (TAT) by standard method and direct MALDI-TOF analysis were compared.