It is not recommended to use cell culture treated plates (TC treated microplates / Cellstar® microplates) in ELISAs. The cell culture treatment (TC treatment) of polystyrene creates highly charged surfaces with high affinity to all kinds of polar proteins. Therefore, the non specific background in the cell culture treated microplates is hard to control. Additionally, the ability of our immunological products (Microlon®) to bind proteins consistently is constantly checked in our quality assurance laboratory by means of ELISA.

White microplates are usually used for luminescence measurements and black microplates for fluorescence measurements. The critical properties in these methods, such as background, autofluorescence or crosstalk are considerably improved by the use of black or white pigmented microplates.

The µClear® microplates are clear bottom microplates with a thin polystyrene film bottom (190 µm +/- 20 µm for a 96 and 384 well formats and 75 µm +/- 10 µm for the 1536 well format).

Commonly, µClear® microplates are used in cell based assays as they allow the microscopic monitoring of the cell growth. Additionally µclear® microplates have increased sensitivity down to 340 nm due to a reduction in background in transmission measurements.

Yes! For the determination of nucleic acid and protein concentrations at 260 nm or 280 nm without background interference, the UVStar® microplates are an ideal alternative to expensive and fragile quartz glass microplates or cuvettes.

The microplate with Cat.-No. 784080 is a 384 well white Small Volume™, solid bottom, cell culture treated, sterile microplate with lid. The plate does not have a clear film as it is a solid bottom microplate. Small Volume™ microplates with a clear film bottom (µClear®) are available in the low base design (= references starting with 788 ...) E.g. Reference 788 093 = 384 well, white, Small Volume™, µClear®, cell culture treated, sterile microplate without lid.

The difference between Small Volume™ LoBase and Hi Base design is the height of the plate (Small Volume™ HiBase = 14.4 mm / Small Volume™ LoBase = 7.5 mm) and the position of the wells. Customer Drawings of both plates can be downloaded from our webpage.

If sterility is not a requirement for the assay, the non treated med binding plates can be used. If the assay should be run under sterile conditions, sterile plates should be used. If no special sterile microplate is listed in our product catalog, e.g. for the black or white solid bottom microplates, we recommend using the high binding plates as these plates are sterile as well due to the high binding treatment .

The stated maximum RCF values are guidelines only, depending on a variety of factors such as rotor, temperature, density, pH and type of liquid. The suitability of tubes for a specific application using high centrifugation forces has to be tested.

1. CELLSTAR®products: These products can be used for general tissue and cell culture. To facilitate cultivation of adherent cells a proprietary physical surface treatment is provided. These products are defined as TC-treated.

2. CELLCOAT® protein coated products: For the cultivation of sensitive cells as well as for specific experimental set-ups Greiner Bio-One is offering Protein-coated cell culture vessels. The surface of these vessels is coated with proteins of the extracellular matrix as well as with synthetic polypeptides. The following protein coatings are available: - Poly-D-Lysine - Poly-L-Lysine - Collagen I - Laminin - Fibronectin

Due to their low protein absorption, PET membranes are very well suited for transport studies. For this kind of application, the pores of the membrane must be small enough to prevent cells from membrane passage. ThinCert™ cell culture inserts with 0.4 and 1.0 µm pores meet this criterion. In general, membranes with a high pore density (translucent) give better results than membranes with a low pore density (transparent).

The thickness of the membrane of ThinCertTM cell culture inserts is graduated according to the size of the integrated pores. It is: 22.6 µm for 0.4 µm pores, 22 µm for 1.0 µm pores, 20 µm for 3.0 µm pores and 15 µm for 8.0 µm pores.

Transparent membranes comprise a lower pore density as compared to translucent membranes. With the different pore densities, the optical appearance also changes. Transparent membranes are optimized for light microscopy.

PET is highly resistant against most organic solvents. When detached from the insert housing, the PET membrane does not curl. Due to high solvent resistance and anti-curling effects the membrane of ThinCert™ cell culture inserts is very well suited for subsequent processing, such as immunohistochemistry.

The hanging geometry of ThinCert™ cell culture inserts ensures that a distance is kept between the insert underside and the well bottom, thus avoiding any damage of cells in the lower compartment during co-culture experiments. Furthermore, spacers at the flanges of the insert avoid any unwanted contact between the insert and the wall of the well.

Poly-L- and Poly-D-Lysine are enantiomers and differ in the sterical assembly of the atoms in the molecule. Both coatings enhance adherence of cells in vitro. Poly-L-Lysine is different than Poly-D-Lysine in that it is degradable by cellular proteases.

Cat.-No. 633180 is a petri dish for bacteriology and is not suitable for cell culture. As this dish does not have a treated surface, cell adhesion is not possible. Furthermore, cell culture products like Cat. No. 633171 are sterile and are regularly checked on cytoxity, freedom of RNase, DNase and human DNA and endotoxin.

The shelf life of Petri dishes includes 4 years for TC-treated variants and 5 years for sterile, but not treated Petri dishes. The shelf life date of the product is printed on the outer label of the carton (hourglass) and is also located for cell culture products on the bag of each product.

Two-position vent stopper fulfils two functions:1.When attached lightly, this enables a uniform ventilation of the tube contents.2.When attached firmly by pressing in the stopper further, an airtight closure of the tube is achieved.

Centrifuge tubes as well as other products of polystyrene are not autoclaveable. Polystyrene is resistant to temperatures between -20 °C and +60 °C. The integrity of Polystyrene products can not be assured when put into contact with high temperature (+120 °C) during the autoclaving process.

All laboratories which manipulate potentially hazardous biological agents and materials, and generate waste containing such agents are responsible for the separation, packaging and treatment of their laboratory waste prior to its removal and disposal.

Greiner Bio One as supplier of disposable plasticware, cannot indicate the appropriate waste disposable measures. The latter being dependent on the respective customer application and country-specific regulations. All Greiner plastic disposables are for single use only and cannot be recycled.

Autoclaving (steam sterilization) is the preferred (and generally regarded as the most reliable) method of sterilizing biological waste. Depending on the volume of waste to be sterilized, it may be necessary to extend the duration of exposure to high temperature steam under pressure.

Steam sterilization is generally not recommended for laboratory waste contaminated with or containing a combination of viable biological agents and significant amounts of hazardous chemical or radioactive materials.

Containers of liquid waste must be placed into an autoclavable tray or pan of sufficient capacity to contain all liquid in the event of vessel failure or breakage inside the autoclave chamber. Use extreme caution when handling autoclaved liquids since they are hot and may boil over.

Autoclavable bags of solid waste should be closed but not sealed airtight to allow steam penetration before they are placed into the autoclave chamber.

It is not recommended to use cell culture treated plates (TC treated microplates / CELLSTAR® microplates) in ELISAs. The cell culture treatment (TC treatment) of polystyrene creates highly charged surfaces with high affinity to all kinds of polar proteins. Therefore, the non specific background in the cell culture treated microplates is hard to control. Additionally, the ability of our immunological products (MICROLON®) to bind proteins consistently and reproducibly is constantly checked in our quality assurance laboratory by means of ELISA.

High binding polystyrene surfaces are hydrophilised using a special method of physical treatment. They display a higher binding capacity for polar molecules. The polystyrene surface of a medium binding microplate is more hydrophobic than the surface of a high binding microplate and is therefore better suited for non-polar molecules.

Greiner Bio-One offers microplates with two different F-Bottom formats - Standard F-Bottom and F-Bottom Chimney Well. Half Area is equal to the F-Bottom format. The smaller well size reduces the sample volume about 50%.

LUMITRAC™ are white microplates. FLUOTRAC™ stands for black microplates. As the assay was developed in white high binding plates (LUMITRAC™ 600, 96 well) our recommendation would be to switch to white 384 well LUMITRAC™ microplates. The LUMITRAC™ 600 plate with the part number # 781074 is a white 384 well high binding microplate we would recommend.

Yes, the Greiner UV Star microplates (# 655801, # 675801 and # 781801) should be compatible with the Varioskan microplate reader as they have a uniform footprint which is conform to the American National Standard Institute (ANSI / SBS Standards 1-2004). Detailed customer drawings with all necessary dimensions for the setup of a new plate definition file can be downloaded from our website www.gbo.com/bioscience under the equivalent part number.These are the PDF-files of the customer drawings:- 655801- 675801- 781801

The 96 well µClear®microplates and 384 well µClear®microplates have a film thickness of 190 µm +/- 10%. In the 1536 well microplate with a transparent bottom (µClear®) the film thickness is 75 µm +/- 10%. UV-Star® microplates generally have a film thickness of 135 µm +/- 10 µm.

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