Product Description

Small GTPases are a super-family of cellular signaling regulators. Rab5 is a member of the Rab-family GTPases. Rab5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway.

Currently there is no direct assay to measure the activation of Rab GTPases.

NewEast Biosciences Rab5B Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab5-GTP, but not Rab5-GDP, and a Rab5B specific rabbit polyclonal antibody. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.

This anti-Rab5-GTP monoclonal antibody can also be used to monitor the activation of Rab5 in cells and in tissues by immunohistochemistry.