Designing and Using Site-Specific Antibodies to Synthetic Peptides

Abstract

Over the past 4 years synthetic peptides have become widely used as “determinants in search of antibodies.” The production of antibodies to small peptides is now common laboratory practice. As with hybridoma technology, the method yields site-specific immunologic reagents. However, the isolation of a monoclonal against any particular determinant is a matter of chance. The identification of the determinant that is recognized by a given monoclonal is a laborious or even impossible task. By starting with a small peptide, the potential determinants are narrowly defined.

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