Stem Cell Culture Reagents and Media

Mesenchymal Stem Cell Basal Medium for Adipose, Umbilical and Bone Marrow-derived MSCs is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system. This low serum (2% FBS) system is designed to support mesenchymal stem cells derived from various normal human tissues including lipoaspirates and umbilical cord. Mesenchymal Stem Cell Basal Medium for Adipose, Umbilical and Bone Marrow-derived MSCs contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of various types of adult stem cells, Mesenchymal Stem Cell Basal Medium must be supplemented with the appropriate cell-specific growth kit. When using this complete media system, the growth of undifferentiated mesenchymal stem cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates.

The Adipocyte Differentiation Toolkit for Adipose-derived MSCs and Preadipocytes contains medium and reagents designed to induce adipogenesis in actively proliferating Adipose-Derived Mesenchymal Stem Cells and Preadipocytes with high efficiency, and to support maturation of derived adipocytes during lipid accumulation. The Toolkit is composed of the following components:

One vial containing 1 mL of AD Supplement for use in initiation of adipocyte differentiation

One bottle containing 5 mL of ADM Supplement for use in maintaining adipocyte differentiation

One bottle containing 100 mL of Adipocyte Basal Medium for use in both initiation and maintenance of adipocyte differentiation

*The Adipocyte Differentiation Toolkit for Adipose-derived MSCs and Preadipocytes provides enough medium and reagents for differentiation of ~6.8 x 105 cells when plated at a recommended density of 18,000 viable cells/cm2 in 4 wells of a 6 well tissue culture format.

Stem Cell Dissociation Reagent is a neutral protease isolated from Bacillus polymyxa that promotes safe and efficient detachment of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) during subcultivation in cell culture. The gentle proteolytic action of the reagent does not damage the cell membrane and supports the growth of hiPSCs in an undifferentiated state. Serum does not inhibit the activity of the reagent. Stem Cell Dissociation Reagent is suitable for hESCs and hiPSCs cultured in feeder-free and feeder-dependent conditions.

This ready to use, complete medium, is tested for pH, osmolality, endotoxins and sterility. Each lot of Pluripotent Stem Cell SFM XF/FF is tested functionally with human stem cells to ensure that pluripotency is maintained as determined by expression of pluripotent markers.

Each lot of CellMatrix Gel is shown to promote the attachment of hESCs/hiPSCs and to maintain hESCs/hiPSCs in a pluripotent state as determined by the expression of Oct-4 and Nanog pluripotent markers.

ATCC® Number:SCRR-2011™Quantity:
500 mL
Storage:
Store medium at 2°C to 8°C in the dark when not in use.
Applications:
General culture conditions to maintain undifferentiated,
pluripotent mouse ES cells are now well established, and
incremental improvements, such as the development of
Mouse ES Cell Basal Medium, are key to the ongoing
success of stem cell research.

The medium contains a stable glutamine dipeptide, providing
a long shelf-life when handled and stored properly medium may be supplemented by addition of ES-Cell
Qualified Fetal Bovine Serum (FBS) or a serum replacement
designed for mouse ES cells.
This medium can support the growth of mouse ES cells on
either a mitotically inactivated layer of mouse embryonic
fibroblast (MEF) feeder cells, or in a feeder-free environment using gelatin-coated plates.