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Bairy KL et al,J Biosci Tech, Vol 3 (1),2012,446- 448 ISSN:0976-0172 EFFECT OF MOMETASONE FUROATE AND BETAMETHASONE VALERATE ON SKIN INFLAMMATORY MODEL IN WISTAR RATS Bairy KL1*, Deepa Ayyappa3, Chandrashekar BR3, Avinash M Holla3, Pawan kumar A.V3 , Purnima Sirigiri3, Avin D’Souza1, Satish Kumar MC2
1. Professor and Head, 2. Assistant professor, 3. MSc student Kasturba, Medical College,
Manipal University, Manipal, Karnataka- 576104, India.
ABSTRACT
There is always a need for better antiflammatory drugs for conditions like atopic
Keywords:
dermatitis. A newer formulation is biopolymer based fluticasone propionate .This
study was done to compare the efficacy of mometasone furoate with biopolymer
with mometasone furoate (Elocon) and betamethasone valerate with biopolymer
with betamethasone valerate(Betnovate) in animal models of superficial skin
inflammation in rats. The inflammatory model croton oil ear edema of topical administration in rats was used. In this model, the animals treated with mometasone furoate with biopolymer showed a significant decrease in inflammation as compared to mometasone furoate (Elocon) and betamethasone valerate with biopolymer as compared to betamethasone valerate (Betnovate).
1. INTRODUCTION
In this study we are comparing the anti-
inflammatory activity of Elocon with a new
Inflammation though a protective formulation mometasone furoate with mechanism, in some situations if untreated
biopolymer on superficial skin inflammation
can lead to serious complications. To know
the efficacy of drugs used in inflammation
various animal models are used. 2. MATERIALS AND METHODS Inflammatory changes can be induced in 2.1. Animals these animals by administration of various
agents and anti-inflammatory efficacy of Adult male Wistar rats weighing between different drugs can be compared. 150-200g were used. Animals were Inflammation is a complex biological acclimatized to the laboratory environment response of vascular tissues to harmful for 5-7 days before entering in the study. stimuli, pathogens or irritants1. Exposure to
They were allowed free access to water and
chemicals, irritants and allergens leads to
were maintained on standard rat diet under
various inflammatory disorders. The laboratory conditions. 12- hour light/dark treatment for such disorder includes cycle was maintained. All procedures were avoidance of allergens, irritants, adequate
carried with approval of Institutional Animal
cutaneous hydration and judicious use of
low to moderate potency corticosteroids. There is always a search for more 2.2. Drugs efficacious preparations.
Elocon (mometasone furoate), mometasone
Fluticasone propionate is moderately potent
glucocorticoid with anti-inflammatory and
immunosuppressive properties. This drug is
available in various forms and one of them
is mometasone furoate marketed as Elocon.
Bairy KL et al,J Biosci Tech, Vol 3 (1),2012,446- 448 ISSN:0976-0172 2.3. Anti-inflammatory studies
significant increase in ear plug weight. The
Croton oil ear edema in rats:
eight treatment groups were compared with
control. The difference in weight between
rats. The irritant croton oil was prepared by
dissolving 4 parts of croton oil, 10 parts of
ethanol, 20 parts of pyridine and 66 parts of
calculated as percent inhibition of edema
ethyl ether. The test compounds were using the equation2 dissolved (5mg/ml strength) in the croton
oil. The animals were divided into Seven
groups of 10 animals each. The control and the test animals were anaesthetized with 4. DISCUSSION ether and then received the drugs in Corticosteroids play an important role in
established that inflammation induced by
Group I - 0.02ml of croton oil solution Group II - 0.02ml of croton oil solution containing
croton oil is related to the activation of
Group III - 0.02ml of croton oil solution containing
arachidonic acid from the cell membrane.
Mometasone Furoate Cream A with biopolymer
Arachidonic acid, in turn, is metabolized to
prostaglandins (PG’s) and leukotrienes.
Group IV - 0.02ml of croton oil solution containing Mometasone Furoate Cream B with biopolymer
Substances able to inhibit edema could be
Group V - 0.02ml of croton oil solution containing
inhibitors of cyclooxygenase (COX) and/or
GroupVI - 0.02ml of croton oil solution containing
action of glucocorticoids is mediated mainly
Betamethasone valerate Cream A with biopolymer (5mg/ml)
Group VII - 0.02ml of croton oil solution containing
phospholipase A2 on the arachidonic acid
Betamethasone valerate Cream B with biopolymer
cascade4 resulting in decreased synthesis of
The drug was applied externally to the outer
In this study there was a significant decrease
surface of right ear of each rat. The animals
in edema in rats treated with Mometasone
were sacrificed by cervical dislocation after
four hours and discs of 8mm punches were
made with a cork borer. Each ear disc was
2.4. Statistical analysis
Results are expressed as mean ± SEM and
were analyzed statistically by analysis of
Biopolymer based drugs play an important
role in development of drug formulations as
3. RESULTS
they have specific advantages6. Biopolymers
Croton oil edema in rats:
are generally nontoxic and biocompatible. It
Topical application of croton oil induced
Bairy KL et al,J Biosci Tech, Vol 3 (1),2012,446- 448 ISSN:0976-0172 The percentage of edema in seven groups Groups (n=10)Mean ± SEM
Elocon 22.92+11.64Mometasone Furoate Cream A
57.87+17.37 35.37+5.76*
43.05+9.40 25.55+5.44*
63.39+16.01
* Significant p< 0.05 is most probably the better pharmacokinetics [7] Biotechnology. Mater Sci Eng R Rep. 2008; 62: of the biopolymers that gives them an
[8] Sinha V.R., Kumria R. Polysaccharides in colon
advantage over the conventional specific drug delivery. Int. J. Pharm 2001; 224:
preparations. In conclusion, advances in drug
delivery improve the pharmacokinetics of promising drugs for many diseases and biopolymers have great potential for delivery of pharmaceuticals. Biopolymer based
formulations can be promising candidates for various types of inflammation in which conventional preparations have shown less efficacy.
5. REFERENCES
[1] Meena MK, Jain AK, Jain CP, Gaur K, Kori ML,
Kakde A etal. Screening of anti-inflammatory and
[2] Analgesic activity of Cassia grandis Linn.
Academic Journal of Plant Sciences 2009; 2: 51- 55.
Antiinflammatory and antispasmodic activity of
Ipomoea imperati (Vah) Griseb (Convolvulaceae)
[4] Benito PB, Abad Martinez MJ, Silván Sem AM,
San Gómez A, Fernández Matellano L, Sánchez Contreras S & Diaz Lanza AM. In vivo and in vitro anti-inflammatory activity of saikosaponins.
[5] Goulding NJ, Guyre PM. Glucocorticoids,
lipocortins and the immune response. Curr Opin Immunol. 1993; 5:108-113.
[6] Chow D, Nunalee ML, Lim DW, Simnick AJ,
Chilkoti A. Peptide based biopolymers in

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