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Description:

Background: Flt-3 ligand is a recently identified hematopoietic cytokine whose activities are mediated by binding to the transmembrane glycoprotein Flt-3. Flt-3 was first discovered as a member of the class III subfamily of receptor tyrosine kinases (RTK) whose expression among hematopoietic cells was found to be restricted to highly enriched stem/progenitor cell populations. Additional class III RTKs include the receptors from SCF, M-CSF and PDGF. Not surprisingly, Flt-3 ligand is also structurally related to M-CSF and SCF. All three cytokines have been shown to exist both as type I transmembrane proteins and as soluble proteins.

Entrez Symbol:

FLT3L

Data PDF:

CRF158

Source:

E. coli

Molecular Weight:

~18.0 kDa

Formulation:

Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.

Purity:

>97% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human AML5 cells is less than 1 ng/ml.

Specific Activity:

＞1.0 × 106 IU/mg

Endotoxin Level:

<1EU/μg of rRhFlt-3L as determined by LAL method.

Amino Acid Sequence:

Reconstitution:

Centrifuge vial prior to opening. We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

The lyophilized protein is stable at 2-4°C, but should be kept desiccated at -20°C for long term storage. After reconstitution, the protein is stable for 1 week at 2-4°C. For long term storage, aliquot and freeze at -80°C. Avoid repeated freeze-thaw cycles.