Looking for team members with expertise in retinal biology or bioprinting retinal tissues.

Also willing to join a larger team; team offers retina surgery expertise and a self-contained extrusion based bioprinting system for printing thick vascular tissues having perfusion channels for providing nutrients. Characterization of the 3D bioprinted tissue is performed using live-dead assay to determine long term tissue viability.

Larry Rizzolo, Yale University:

I have developed a novel scaffold for growing a planar, laminated neo-retina that can be cultured with RPE in its natural orientation. I am looking for expertise in scaling up the process to develop a platform for drug screening.

Nandor Garamszegi, Natural to Artificial Genomes and Biosystems:

For NEI 3D ROC Implementation Phase, we are looking for additional team members/organizations with advanced analytical equipment as spectroscopy/mass spectroscopy, confocal microscopy, advanced N2/O2 incubation capability for long term organoids, and functional retinal model bioactivity readouts as for example patch clamp technology. Additionally, team/organization members with single cell NGS (transcriptomics, RNAseq, gene expression and metabolomics) technologies for validating cell lineages and track down disease molecular mechanisms will be extremely useful and are welcome.

The Biofabrication Center has developed a 3D organoid printing approach that uses Liquid Like Solid (LLS) materials to enable precise scaffold-free 3D printing of microtissues in unrestricted 3D co-culture. This approach provides both a continuous solid support for culture of printed tissue constructs, handling, removability, and controlled fluid transport.

I'm interested to join a team working on the assembly of organoids or organoid-derived cells in larger 3D cellular structures. For this, I am offering collaborative or independent access to the Regenova bioprinter operated on a spheroid-based, ‘scaffold-free’ method.

Jay Gopalakrishnan, University of Cologne:

We generate iPSC-derived human brain organoids. Lately, we generated human brain organoids with fore-brain specific region. Further differentiation resulted in two eyecups. These organoids have photoreceptors and thus are light sensitive. We could now generate such organoids from RP patients to model diseases and to use them for transplantation experiments.

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