Applications/Dilutions

Dilutions

Western Blot

Flow Cytometry

Immunocytochemistry/Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Application Notes

This PHD2 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Western Blot, and Immunohistochemistry-paraffin embedded sections. In ICC/IF, cytoplamic and nuclear staining was observed in HeLa cells. Immunoprecipitation was reported in scientific literature.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Theoretical MW

46 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage

Store at 4C in the dark.

Buffer

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Purity

Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for EGLN1/PHD2 Antibody [DyLight 405]

C1orf12

C1orf12HPH2

EC 1.14.11

EC 1.14.11.-

ECYT3

egl nine homolog 1 (C. elegans)

egl nine homolog 1

egl nine-like protein 1

EGLN1

HIF prolyl hydroxylase 2

HIFPH2 EGL nine (C.elegans) homolog 1

HIFPH2

HIF-PH2

HIF-prolyl hydroxylase 2

HPH2

HPH-2

Hypoxia-inducible factor prolyl hydroxylase 2

PHD2

PHD2SM20

Prolyl hydroxylase domain-containing protein 2

SM20

SM-20

zinc finger MYND domain-containing protein 6

ZMYND6

ZMYND6DKFZp761F179

Background

PHD2 is one of 4 PHD (PHD1-4) enzymes that function as oxygen sensors and are responsible for the post-translational modification of HIF-1alpha, a component of a transcriptional complex involved in oxygen homeostasis. During normoxic levels, PHDs catalyze the hydroxylation of prolyl residues on HIF-1alpha and target it for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. The PHD isoforms appear to function in a non-redundant manner and may differ in their expression patterns and their catalytic selectivity. Alternate names for PHD2 include PHD2, PH2, Prolyl hydroxylase domain containing protein 2, HIF2PH2, HIF-Prolyl hydroxylase 2, EGLN1, egl nine homolog 1, and C1orf12.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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