Netrin-1 (mouse), (recombinant)

Product Specification

Produced in HEK 293 cells. The extracellular domain of mouse netrin-1 (aa 27-604) is fused at the C-terminus to a FLAG®-tag.

UniProt ID:

O09118

Concentration:

0.1mg/ml after reconstitution.

Formulation:

Lyophilized. Contains PBS.

Purity:

≥90% (SDS-PAGE)

Purity Detail:

Purified by multi-step chromatography.

Endotoxin Content:

<0.1EU/µg purified protein (LAL test; Bio Whittaker).

Species reactivity:

Human, Mouse

Specificity:

Binds to human and mouse UNC5B.

Applications:

ELISA

Application Notes:

ELISA: binds to UNC5B.

Reconstitution:

Reconstitute with 100µl sterile distilled water. Resuspended protein should be used immediately upon reconstitution to avoid loss in activity due to non-specific binding to the vial. Further dilutions should be made with medium containing 5% fetal calf serum or 0.5% BSA.

The Netrins (whose name means “one who guides” in Sanskrit) are a family of laminin-related secreted protein. In mice, human and rat, netrin-1, netrin-3/NTL2, Netrin-4 and G-Netrins have been cloned. Netrin-1 is predominantly expressed by floor plate cells as commissural axons extend toward the ventral midline. Netrin-1 induces axonal outgrowth, axon orientation and neuronal migration in neuronal development. It is chemoattractive for some neurons and chemorepellent for others. To carry its functions, netrin-1 interacts with specific receptors: DCC/Neogenin and UNC-5. These receptors belong to the family of “dependence receptors” that induce apoptosis in the absence of their respective ligand, and promote survival when bound to their ligand. The Netrin-1 mediated survival signal inhibits p53-induced apoptosis. Netrin-1 is also expressed in organs such as pancreas, lung, bowel, bone and mammary gland. In these tissues, netrin-1 has different roles such as invasion of epithelial cells, leukocytes migration, cell adhesion or angiogenesis. Netrin-1, as a survival factor, also contributes to the control of tumorigenesis.