How to add keystore (Signature) in soap call

How to add keystore (Signature) in soap call

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Hi -
I am using JMeter 3.1 version, I have created "Test Plan" and created a "Thread Group" then add the HTTP Request then add the "Keystore Configuration", "SOAP Message Signer" and "HTTP Header Manager". But still am request was not signed. Am getting an Response code: 500 Response message: Internal Server Error.

Also, I have tried to create SOAP/XML-RPC Request Test plan, getting same error in this way as well.