Fig. 6

CKS2 promotes pancreatic cancer cell proliferation. a Significant pathways enriched in genes whose expression profile matches that of CKS2, based on DAVID software. bCKS2 mRNA levels in pancreatic cancer tissues and paired normal tissues from 102 patients with PDAC at our hospital, based on real-time PCR analysis. Results are mean ± SEM. *P < 0.05. c Western blotting of CKS2 in PDAC cell lines BxPC-3 and CFPAC-1, as well as in non-cancer pancreatic duct epithelial cell line HPDE6-C7. d Western blotting of CKS2 in PDAC (T) and paired normal tissues (N). Representative results of 20 pairs of clinical specimens are shown. eCKS2 knockdown in BxPC-3 and CFPAC-1 cells. Levels of mRNAs were determined by qRT-PCR. Results are mean ± SEM from three independent experiments. fCKS2 knockdown substantially reduced proliferation of BxPC-3 and CFPAC-1 cells, as determined using the CCK-8 assay. Results are mean ± SEM from three experiments, each conducted with six replicates of each sample. ***P < 0.001 vs. control