Hi -
I have a question about quantitating northerns on a phosphoimager.
I've been blotting total RNA, probing w/a 32P labeled transcript and
scanning into a phosphoimager. After quantitating, I find myself
wondering about some of the numbers that the software spit out.
Should I expect the signal I get from 5 ug of total RNA to be half
the signal I'd get from 10 ug? Empirically, I'm not seeing this and
have been wondering why, or, like any other scientist, "what did I do
wrong?"
Thanks in advance,
Robert
Robert A. Schoenfeld, Ph.D.
UC Davis
MCB, rm. 24 Briggs Hall
Davis, CA 95616
phone: 530-752-2938
fax: 530-752-3085
email: raschoenfeld at ucdavis.edu
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