The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum
and 2015_04 (r6.07) for Drosophila melanogaster.

GO terms for the homologous Drosophila genes

Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms.
Search AmiGO for the correct GO ID.
Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked).
Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge.
We will review this information and submit the annotation to the Gene Ontology consortium.

Gene

{{ goGene }}

The Tribolium gene the GO term is assigned to.

GO ID

*

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The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'.
This information is mandatory.

Evidence

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The evidence code describes with which method the function or
location, expressed by the GO term, was show for this gene.
Please refer to this page
for a definition of the evidence code and a decision tree.

Reference

*

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The reference where the function or location is described for the gene.
Please enter a PubMed ID. This information is mandatory.

Quotation

If you paste here the relevant section of the reference it is
for us a great help to review your annotation. The quotation may
be also be displayed to the users of iBeetle-Base.

Name

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to clarify any questions. If you agree, we may show your name
on this page to honor your contribution.

Email

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regarding this annotation. We will never show your address
or give it away.

Publish name

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we might show your name on this page to honor your contribution.

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* : mandatory fields

For help on how to submit larger datasets or non experimental data please contact us.

iBeetle screen iB_01947

Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments!
Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

iB sequence

General information

iB number

iB_01947

iB fragment

The sequence from the iB fragment is matched against the genes from the
official gene set. The matche(s) is/are visualizied in a genome browser.

Pupal injection procedure

3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

Note: The adult morphology was not analyzed systematically at 3/9/11 dpi.
Only obvious phenotypes, visible without magnification, have been annotated.

13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

Terms used in the pupal injection procedure

At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.

empty eggs

Just empty shells are visible, no cuticle has been developed.

strong defects

L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories

strongly fragmented (cuticle crumbs)

No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.

cuticle remnants (with unidentifiable segments)

Segments or parts of appendages are recognizable, but identity is not clear.

cuticle remnants (with some identifiable segments)

At least one segment or appendage is clearly regonizable.

number of eggs on slide

affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed.

total number of affected eggs/embryos/larvae

Summary of all different phenotypes/ phenotypic series which were annotated.

inside-out

The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.

starved

Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.

eclosion not fulfilled

The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.

Terms used in the larval injection procedure

At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.

pupal molt not fulfilled

The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.

eclosion not fulfilled

The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.

starved

Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.

Metamorphosis and survival

Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)