Staurosporine induces apoptosis in Jurkat cells, as measured by nuclear morphology, a decrease in mitochondrial membrane potential, and an increase of Annexin V FITC positive cells.Panels A-C show same field of cells from the control group whereas panels D-F show the same field of cells from the staurosporine-treated group.Hoechst staining shows that most control cells have round and intact nuclei (Panel A) whereas staurosporine-treated cells mostly have condensed and fragmented nuclei (Panel D). TMRE staining reveals that most control cells have undisrupted mitochondrial membrane potential (Panel B) whereas staurosporine-treated cells mostly have diminished membrane potential and were not stained (Panel E). Most control cells are Annexin V negative (Panel C) whereas cells treated with staurosporine are mostly Annexin V positive (Panel F), indicating cells are undergoing apoptosis.

Functional Study

Flow Cytometry

Staurosporine induces apoptosis in Jurkat cells, as measured by nuclear morphology, a decrease in mitochondrial membrane potential, and an increase of Annexin V FITC positive cells.Panels A-C show same field of cells from the control group whereas panels D-F show the same field of cells from the staurosporine-treated group.Hoechst staining shows that most control cells have round and intact nuclei (Panel A) whereas staurosporine-treated cells mostly have condensed and fragmented nuclei (Panel D). TMRE staining reveals that most control cells have undisrupted mitochondrial membrane potential (Panel B) whereas staurosporine-treated cells mostly have diminished membrane potential and were not stained (Panel E). Most control cells are Annexin V negative (Panel C) whereas cells treated with staurosporine are mostly Annexin V positive (Panel F), indicating cells are undergoing apoptosis.