Table 1: ELISA. Ferritin is present in human serum and undetectable in goat serum. Spike controls indicate no interference in absorbance readings from the diluent used to prepare standards and sera samples. Absorbance readings (OD450 nm)are shown for standard curve, spike controls and unknown samples. Value for avg reading is derived from the average reading of three samples. Avg reading for 0 ng/ml standard was subtracted from all avg readings to yield corrected OD450 nm values for standards, spike controls and unknown samples. Standard deviation is included for all samples. Standard curve was generated by regression analysis with four-parameter logistic. An equation (y = 0.1983x4 - 0.771x3 + 1.2081x2 + 2.1927x + 0.0425 ) was derived from the standard curve and used to calculate Ferritin concentrations shown in Table 1.

Diagramm of the ELISA kit to detect Human FEwith the optical density on the x-axis and the concentration on the y-axis.

Table 1: ELISA. Ferritin is present in human serum and undetectable in goat serum. Spike controls indicate no interference in absorbance readings from the diluent used to prepare standards and sera samples. Absorbance readings (OD450 nm)are shown for standard curve, spike controls and unknown samples. Value for avg reading is derived from the average reading of three samples. Avg reading for 0 ng/ml standard was subtracted from all avg readings to yield corrected OD450 nm values for standards, spike controls and unknown samples. Standard deviation is included for all samples. Standard curve was generated by regression analysis with four-parameter logistic. An equation (y = 0.1983x4 - 0.771x3 + 1.2081x2 + 2.1927x + 0.0425 ) was derived from the standard curve and used to calculate Ferritin concentrations shown in Table 1.

Diagramm of the ELISA kit to detect Human FEwith the optical density on the x-axis and the concentration on the y-axis.

Table 1: ELISA. Ferritin is present in human serum and undetectable in goat serum. Spike controls indicate no interference in absorbance readings from the diluent used to prepare standards and sera samples. Absorbance readings (OD450 nm)are shown for standard curve, spike controls and unknown samples. Value for avg reading is derived from the average reading of three samples. Avg reading for 0 ng/ml standard was subtracted from all avg readings to yield corrected OD450 nm values for standards, spike controls and unknown samples. Standard deviation is included for all samples. Standard curve was generated by regression analysis with four-parameter logistic. An equation (y = 0.1983x4 - 0.771x3 + 1.2081x2 + 2.1927x + 0.0425 ) was derived from the standard curve and used to calculate Ferritin concentrations shown in Table 1.

Diagramm of the ELISA kit to detect Human FEwith the optical density on the x-axis and the concentration on the y-axis.

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Ferritin Antigen Profile

Beschreibung des Gens

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes.