To link to the entire object, paste this link in email, IM or documentTo embed the entire object, paste this HTML in websiteTo link to this page, paste this link in email, IM or documentTo embed this page, paste this HTML in website

IDENTIFICATION OF STRAIN-SPECIFIC DNA FRAGMENTS OF
ACTINOBACILLUS ACTINOMYCETEMCOMITANS BY REPRESENTATIONAL
DIFFERENCE ANALYSIS
by
Johanna Mena
____________________________________________________________________
A Thesis Presented to the
FACULTY OF THE GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(CRANIOFACIAL BIOLOGY)
May 2007
Copyright 2007 Johanna Mena

A genomic subtraction method, the representational difference analysis (RDA), was tested for its use in identifying strain-specific DNA in A. actinomycetemcomitans. Subtraction of strain D7S with strain HK1651 yielded D7S-specific 2.3 kb islet-A and 5.3 kb islet-B. Islet-A contains a 1.5 kb region that is homologous to a region found in the A. actinomycetemcomitans plasmid pVT745. Islet-B contains a 2.1 kb homolog of vgr, a component of a DNA repeat element rhs. The distribution of these islets among A. actinomycetemcomitans strains was further examined by PCR. Islet-A was found in 9 serotype a strain and 2 serotype b strains but missing in 37 strains. Islet-B was found in 1 serotype a strain, 4 serotype d strains and 2 serotype e strains, but missing in 41 strains.

IDENTIFICATION OF STRAIN-SPECIFIC DNA FRAGMENTS OF
ACTINOBACILLUS ACTINOMYCETEMCOMITANS BY REPRESENTATIONAL
DIFFERENCE ANALYSIS
by
Johanna Mena
____________________________________________________________________
A Thesis Presented to the
FACULTY OF THE GRADUATE SCHOOL
UNIVERSITY OF SOUTHERN CALIFORNIA
In Partial Fulfillment of the
Requirements for the Degree
MASTER OF SCIENCE
(CRANIOFACIAL BIOLOGY)
May 2007
Copyright 2007 Johanna Mena