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J. FEDER (Monsanto Company, St. Louis, MO): Does your medium contain serum? DR. R. G. RUPP: Yes, there's 5% horse serum. We recently reduced that significantly though. With the continuous feed there's no need to put so much serum in the medium. DR. A. J. PARCELLS (Upjohn, Kalamazoo, M I ) : You mentioned that you are using two different anion exchangers, trisacryl and Whatman. Do you have any preference of one over the other? DR. R. G. RUPP: Yes, I like trisacryl. It doesn't compress when you strip it and it binds pyrogens if they're there.

RUPP: No. We were given certain parameters under which we had to work and since we were planning to produce a number of patient-specific antibodies, we needed to cram as many of these vessels into as small a space as possible. Load cells became somewhat prohibitive. DR. J. , Thousand Oaks, CA): Have you looked at the viability of the cells inside the beads with time? It appears that you had a constant rate of production of antibody even though the cell density was increasing, suggesting that the specific productivity was decreasing.