ªRãwïActa Medica Okayama0030-1558717-11959×ÛãÓÉÎ·éILVCu, CoöÌe¿ æ2Ò ILV, Cu(++), Co(++)ÌYÁ|n­çÛÌyfI«ó37553764ENYukioEguchiWith the use of standard strains of Sh. flexineri and Staphy. aureus taken from the departmental stock, the author carried out the serial transplantation through generations on the media that was added oxine, Cu(++), Co(++), the combination of oxine and Cu(++) or that of oxine and Co(++) in order to obtain bacteria adapted to respective media. Then the investigation was made on the properties of the bacteria thus obtained. The results are following. 1) It was found the decreased activity of catalase by the bacteria grown on ox ine added media, but that activites of other other bacteria were maintained nearly normal. 2) It was revealed slight defferences between the permeabilities of inhibitor through the surface of bacterial cells cultured on different media. 3) The inhibitory effects of Cu(++) on O(2) uptake were varied by the bacteria cultured on different media added respectively oxine, Cu(++) or oxine+Cu(++). That effects of Co(++) were also varied by the bacteria on different media added respectively oxine, Co(++) or oxine+Co(++) 4) There was found no difference on the pathway of glucose metabolism resulting in pyruvate, but was noticed some difference in further oxydation of pyruvate.No potential conflict of interest relevant to this article was reported.ªRãwïActa Medica Okayama0030-1558717-11959×ÛãÓÉÎ·éILVCu, CoöÌe¿ æ1Ò ­çÛyÑÃ~ÛÉÎ·éìp37473754ENYukioEguchiUsing the standard strains of Sh. flexneri and Staphy. aureus taken from the departmental stock, the author studied the effects of oxine, Cu(++), Co(++), combination of oxine-Cu(++) and that of oxine-Co(++) on the growth of bacteria and the glucose metabolism during their growth. The following results were obtained. 1) The cooperative inhibition of oxine and Cu(++) or oxine and Co(++) on the growth was revealed most strikingly when oxine and metal ions came near equal concentrations. Of course, it was necessary to be kept both oxine and metal ions are exceeded a certain concentrations for revealing their inhibitory effects. 2) The accumulation of pyruvate and lactate in the media was markedly increased in oxine or Co(++) added media, on the other hand the accumulation of the metabolites from glucose was decreased in the case of Cu(++) added media compaired with control. 3) It was found that the metabolite acumulated slightly much in the media added oxine and Cu(++) or oxine and Co(++) than control media.No potential conflict of interest relevant to this article was reported.