[Histonet] Co-localization studies

From:

AGrobe2555@aol.com

In your case, if you do 2 rounds of staining and detect each primary
individually with a different-colored secondary, you should have no problem. It is
indeed more convenient if the primaries are from different species, as you
can detect both simultaneously with different-colored secondaries. This saves
quite a bit of time. You could also used direct-conjugated primary
antibodies, but the sensitivity may be reduced.
Albert
Albert C. Grobe, PhD
International Heart Institute, Tissue Engineering Lab
Saint Patrick Hospital
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