FLIP Antibody (PA5-19881)

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Product Details

Bioinformatics

Documents

A peptide corresponding to amino acids 449 to 465 of mouse FLIPL/CASHa.

Conjugate

Unconjugated

Form

Liquid

Purification

Ion-exchange chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage Conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Tested Applications

Dilution *

Immunocytochemistry (ICC)

5 µg/ml

Western Blot (WB)

0.5 µg/ml

* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the
product for use in their own experiment using appropriate negative and positive controls.

Product Specific Information

In Western blot applications, this antibody detects a band at ~55kDa. A suggested positive control is NIH-3T3 cell lysate.

PA5-19881 can be used with blocking peptide PEP-0013.

Background/Target Information

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED-3 protease family.Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP respectively. The human FLIP was also cloned by several labs independently and termed Casper , I-FLICE, FLAME-1, CASH, CLARP and MRIT. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.