No cross reaction with HCV polyprotein core region and other non-structural regions.

Host

Mouse

Clonality

Monoclonal

Clone

70-13

Isotype

IgG1,k

Target Name

HCV NS3

Antigen Species

HCV

Immunogen

A highly antigenic polypeptide consisting of essential sequences of at least 60 residues in length, which were selected from genes encoding the NS-3 region of a Chinese HCV strains

Conjugation

Un-conjugated

Application Instructions

Application Suggestion

Application

Dilution

ELISA

Assay-dependent

WB

4 μg/ml

Application Note

Western Blot: mAb 70-13, at a concentration of 4μg/mL will allow visualization of 200 ng/lane synthetic NS-3 protein. The mAb works on blots transferred from both reducing and non-reducing PAGE gel.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form

Liquid

Purification

Protein G affinity purified

Buffer

0.01M PBS (pH 7.2)

Concentration

1 mg/ml

Storage instruction

For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Note

For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background

Hepatitis C virus (HCV) causes chronic hepatitis and liver cirrhosis in human through blood and body fluid transmission. HCV has a positive sense single RNA genome enclosed in the nucleocapsid made of core protein (capsid protein). The nucleocapsid is covered by an envelope made of lipoproteins (E1 and E2). The 9.6 kb HCV genome has a single open-reading frame, which is to be translated into a single polyprotein. HCV viral proteins are produced after processing the polyprotein. Genes for core protein and envelop proteins are located adjacently at the 5’-end of HCV genome, followed by genes for non-structural proteins including NS2, NS3, NS4A, NS4B, NS5, NS5A and NS5B.