An immersive stereoscopic three-dimensional (S3D) viewing experience is constructed on a successful scene design of the content. Colour is a remarkable factor that advantages human depth perception. Previous works in colour and depth perception have shown that long-wavelength stimuli, such as red or yellow, compete with short-wavelength stimuli such as blue or green, reds and yellows appear closer than blues or greens. Saturation and brightness are considered influential in spatial design. High saturated colours tend to capture more attention than neutral colours, and brighter colours appear advance while darker colours appear receded. This research explores stereoscopic depth perception based on investigating previous findings in colour and depth perception under a current 3D cinema viewing condition. The effect of colour on stereoscopic depth perception are examined from psychophysical and depth quality viewpoints. It considers the way in which depth perception is influenced by different decisions of hue, saturation and brightness, and identifies the thresholds and depth quality of stereoscopic depth perception in different colour arrangements. In particular, different levels of hue, saturation and brightness are tested on foreground and background colour palettes in computer-generated scenes. Psychophysical trials are utilised to examine the thresholds of observers’ depth perception. A depth quality assessment is then performed to evaluate different colour arrangements in practical scenes. A polarised projection system is built for stereoscopic viewing and the image stimuli are designed and rendered in the industry-standard 3D application package. The results indicate that hue, saturation and brightness are effective in stereoscopic depth perception. However, the discriminations of depth perception are equally efficient between the standard and reversed trials in hue and saturation section, which given an inference that contrast is a more dominant value in stereoscopic depth perception. Brightness is also a dominant factor but it can be resulted an undesired depth quality if the high brightness arranged in the foreground.