アプリケーション

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション

Abreviews

特記事項

ICC/IF

Use a concentration of 5 µg/ml.

WB

Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 38 kDa).

ターゲット情報

機能

Receptor for extracellular ATP > UTP and ADP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. May be the cardiac P2Y receptor involved in the regulation of cardiac muscle contraction through modulation of L-type calcium currents. Is a receptor for leukotriene B4, a potent chemoattractant involved in inflammation and immune response.

組織特異性

Expressed at highest levels in heart, skeletal muscle and at lower levels in brain and liver. High level of expression in lymphoid tissues.

配列類似性

Belongs to the G-protein coupled receptor 1 family.

翻訳後修飾

Phosphorylated by GRK6 upon leukotriene B4 binding; which promotes desensitization.

ICC/IF image of ab82851 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82851, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 5µg/ml.

Thank you for submitting your review for ab82851 for western blotting of rat samples. I have compared the immunogen sequence use to produce ab82851 with the that of the rat sequence. Unfortunately they are quite divergent so I would not expect this ant...

I am very pleased to hear you would like to test ab82851 in mouse or rat. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for mouse or rat and include this code in the “Additio...