Beta-alanine supplementation has been shown to improve exercise performance in short-term high-intensity efforts. However, whether supplementation with beta-alanine is ergogenic to actual sporting events remains unclear and should be investigated in field testing or race simulations. PURPOSE: The aim of this study was to assess if beta-alanine supplementation could improve 2000 m rowing ergometer performance in well-trained male rowers. METHODS: Participants (n=16) completed duplicate trials (2 x pre-supplementation and 2 x post-supplementation) of a 2000 m rowing ergometer race, separated by 28 days of either beta-alanine (n=7; 80 mg·kg-1BM·day-1) or placebo (n=9; glucose) supplementation. RESULTS: Beta-alanine group (pooled) race times improved by 2.9±4.1 s and placebo group slowed by 1.2±2.9 s, but these results were inconclusive for performance enhancement (p=0.055, ES=0.20, SWC=49% beneficial). Race split times and average power outputs only significantly improved with beta-alanine at the 750 m (time -0.7 s, p=0.01, power +3.6%, p=0.03) and 1000 m (time -0.5 s, p=0.01, power +2.9%, p=0.02) distances. Blood La- and pH values post-race were not different between groups before or after supplementation. CONCLUSIONS: Overall, 28 days of beta-alanine supplementation with 80 mg·kg-1BM·day-1 (~7 g·day-1) did not conclusively improve 2000 m rowing ergometer performance in well trained rowers.