Methods: The retinal neuron of postnatal SD rats were cultured in vivo, the elevatedpressure was produced after 7days, and the total RNA was extracted after another 2 days,expression of p53、MDM2 and Ref1 gene were analyzed with RT-PCR.

RESULTS:The expressions of IL-6 after continuous pressure-loading for 16 and 24 hours in the pressure-loading group were(143.1±0.42) and(49.46±1.01) ng/L,which were obviously higher than those in the pressure-loading control group [(18.36±0.43),(18.78±0.50) ng/L,P < 0.05].

Compression control and its significance in the manufacture and effects on properties of poplar LVL

Twenty—four rabbits, after artificial fracture, were randomly allocated into the nut—stress control group and the five groups in which the forces of one times. two times, three times, four times and five times of their body weights were respectively adopted to do the experiment of nut—stress on the artificial fractures. Judging from the 6 healing data of the stability of the broken ends, the gaits in the sixth week, x—ray examination, histology the calcium deposition and curve intensity, it proved that the healing...

Twenty—four rabbits, after artificial fracture, were randomly allocated into the nut—stress control group and the five groups in which the forces of one times. two times, three times, four times and five times of their body weights were respectively adopted to do the experiment of nut—stress on the artificial fractures. Judging from the 6 healing data of the stability of the broken ends, the gaits in the sixth week, x—ray examination, histology the calcium deposition and curve intensity, it proved that the healing effect is the best favorable under the compression of three times and four times of body weights and that the best shearing stress of nut—stress on the artificial fractures of the rabbits:σ=3.45MPa

AIM:To study the pathological mechanism of sensory disturbance caused by cauda equina nerve damage.METHODS:Fifty pure breed healthy male New Zealand rabbits were devided into th ree groups randomly: non-treatment group(10 rabbits);non-compression control g roup(10 rabbits) and trial group(30 rabbits).At the following time points(1/4,1/ 2,3,7 and 15 days).According to the of the compressor screw the sagittal diamete r of vertebral canal,the model group was divided into 1/9,2/9,1/2.The compressio n devices were...

AIM:To study the pathological mechanism of sensory disturbance caused by cauda equina nerve damage.METHODS:Fifty pure breed healthy male New Zealand rabbits were devided into th ree groups randomly: non-treatment group(10 rabbits);non-compression control g roup(10 rabbits) and trial group(30 rabbits).At the following time points(1/4,1/ 2,3,7 and 15 days).According to the of the compressor screw the sagittal diamete r of vertebral canal,the model group was divided into 1/9,2/9,1/2.The compressio n devices were fixed between S2 and S3 to compress cauda equina.Posterior root g anglion was removed from the model with cauda equina nerve syndrome at different time point,and stained with HE.The number of normal sensory neuron cells were c ounted.RESULTS:After cauda equina nerve syndrome occurred for 1/2 day,there was isch emia and edema in two sides of posterior root ganglion and cellular necrosis of neurons in ganglion.The number of normal cells in posterior root ganglion was 3 and 7.The left in unoperation control group at time point of 30d was 32.2±4.2,3 2.2±4.3,32.2±4.3/mm2; The left in uncomprssion operation group was 32.2±2.3,3 2.2±4.3,32.2±3.1/mm2;The left in experiment group was 17.2±3.3,14.2±3.2,14. 1±2.3/ mm2.There was significant difference between groups(P< 0.05).CONCLUSION:Sensory neurons in posterior root ganglion are very sensitive and e asily suffer necrosis,which is one of the important pathological changes of dire ct degeneration in cauda equina nerve damage and also explain why the numbness o f sella turcica region and sensory disturbance are hard to recover.

Purpose: To investigate the expression of p53、MDM2 and Ref1 gene in cultured retinaneurons of SD rats treated with Vitamine B1 and (or) elevated pressure.Methods: The retinal neuron of postnatal SD rats were cultured in vivo, the elevatedpressure was produced after 7days, and the total RNA was extracted after another 2 days,expression of p53、MDM2 and Ref1 gene were analyzed with RT-PCR.Results: The expression level of p53 and MDM2 gene were increased in elevatedpressure group, normal with Ref1 gene expression.But...

Purpose: To investigate the expression of p53、MDM2 and Ref1 gene in cultured retinaneurons of SD rats treated with Vitamine B1 and (or) elevated pressure.Methods: The retinal neuron of postnatal SD rats were cultured in vivo, the elevatedpressure was produced after 7days, and the total RNA was extracted after another 2 days,expression of p53、MDM2 and Ref1 gene were analyzed with RT-PCR.Results: The expression level of p53 and MDM2 gene were increased in elevatedpressure group, normal with Ref1 gene expression.But the expression of p53 and MDM2gene were decreased significantly in elevated pressure group treated with vitamine B1compare to the elevated group.Conclusion: Apoptosis seem to be a mechanism of cell death in retinal neurons of SDrats with elevated pressure.Vitamine B1 have protect effects against elevated pressure.