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One-step White Blood Cell Separation from Whole Blood

Abstract:Isolation of White Blood Cells (also called “WBC”) from whole blood is a first very important step for many in vitro assays. One currently accepted technique for WBC separation, referred to as the conventional manual method (FICOLL™, HYPAQUE™) that employs a liquid density gradient medium of FICOLL™ and sodium metrizoate or sodium diatrizoate solution1. Although this commercial available method is very useful in vitro assays, it has many shortcomings because process has many manual steps as well as often time-consuming process. First, blood manually carefully layered onto the medium. Second, isolated WBC is harvested by very carefully pipetting them from the liquid interface after centrifugation. As a result, manual process become reasons of error of the cell separation yield.