It's ending a sentence with a comma and "Bitch!" (no other swear word, just "bitch"), as a way of verbally making an exclamation point in dialog.

It did get overused, so now it's a Discredited Trope. You're as likely as not to see it thrown in a sentence just to be funny, even though there is no other reason to emphasize a point this way. Bitch.

This does not count when merely calling someone a bitch. This is about using it as a form of verbal punctuation. If there is anything between the comma and "bitch", it doesn't count.

Inverted by David Cross, on Bigger and Blackerer: He details a game to play with friends at a restaurant. When talking to the waitress, make the first thing you say be "Yeah, bitch," But in as low-key a manner as possible. Then, be as polite as possible when making your order. The object is to make it so that the waitress won't be sure you called her 'bitch', but would feel uncomfortable asking such a polite person if he did.

Comic Books

From DC, Donna Troy is fighting an alternate-version of herself. When asked who she is, she punches that version in the head and says:

Donna Troy": I'm Donna Troy, bitch. Donna. Troy.

Scott Pilgrim and the Infinite Sadness had the vegan police announce Todd Ingram had eaten "Gelato, bitch!" The movie puts its own spin on it.

Todd: Gelato isn't vegan? Vegan policeman: It's milk and eggs, bitch.

Amanda Conner when she beats up Harley and Catwoman in a fourth wall breaking moment in Harley Quinn #0:

In Evolution, during the mall scene, a woman is putting on sweaters and blouses in the fitting room, obviously shoplifting, when someone keeps knocking on the door. Finally, fed up, the woman says something like "I said I'll be out in a minute, bitch." Then she opens the door... and is faced with a dragon-like alien.

Bad Company had this exchange between Gaylord Oaks (Anthony Hopkins) and Jake Hayes (Chris Rock).

Gaylord Oaks:[after trying to guilt Jake into coming back] Well, that didn't work did it? Jake Hayes: No! [mocking Oaks] "I'm going to go outside and count to ten"? Who the hell are you? You'd would have done better with "Bitch, get in the car!" Gaylord Oaks: Okay. Get in the car... bitch.

Vet Hard: Bennie ends almost every sentence towards Katja with "..., bitch!". Downplayed though since he actually says in Dutch trut which is less of an insult than "bitch" (what the English sub-titles translated it to), and more along the British-English tart.

On Saturday Night Live, any conversation Tracy Morgan holds with Lorne Michaels is likely to end with the former demanding, "Get me a soda, bitch!"

Dave, a contestant on season one of reality cooking show Top Chef, reached the height of reality TV popularity after telling another contestant Tiffani "I'm not your bitch, bitch!" in response to endless haranguing by the latter.

That doesn't quite meanwhat he wanted. "Chiennes" literally means "female dogs." If he wanted "bitches" in the slang sense, he should have used "salopes" or "garces" depending on personal preference.

Wanda Syke's "I'ma Be Me" had her saying "Yeaaah... Obama, bitch!"

Modern Family: "See, we're all going to get past this and be friends again, and this was all set up by me, Mitchell, who is not a mummy's boy but is instead a insightful and considerate person, so make a note bitches!" in episode 4.

That's Just Me: Elizabeth once disguised herself as Amelia and walked into Amelia's house yelling: "I'm your worst nightmare! I'm a motherfucking crossdressing lesbian Jew, BITCH!!". However, is most versions of this episode, the line is censored, changing it into: "I'm your worst nightmare, because I'm a crossdressing tomboy, man!"

From Spartacus: War of the Damned, when warrior-woman Saxa is confronted by two large gladiators who want to kill some prisoners entrusted to her by Gannicus...

Donar: "I do not see Gannicus among you. No cause to heed his bitch." *Attacks. Ends up with Saxa's knife at his throat and his buddy on the ground.*

Saxa: "I give cause, bitch."

In season 1 of Top Chef, Dave told Tiffany after Restaurant Wars "I'm not your bitch, bitch!"

Edward Norton: I'm ordering uniaxial tests of the slab transverse to the deck corrugations with additional reinforcement and tension tests of the support fastener connections between the deck and the girders, bitch!

On The 100, Octavia is the first person from the Ark to set foot on the ground, and she celebrates the moment by shouting, "We're back, bitches!"

On November 5th, 2017, New Japan Pro Wrestling's Power Struggle event ended with Kenny Omega in the centre of the ring flanked by the Young Bucks, having just beaten Trent Barreta to retain his IWGP US Title. After bemoaning the fact that he's exhausted the list of possible challengers - whether Japanese or otherwise - he goes into his customary "Goodbye and goodnight!" sendoff, only for the arena lights to go out. While the music which accompanied the video promo that ensued may have been unfamiliar to some of the NJPW fans in Osaka, the man who appeared on-screen definitely wasn't.

Chris Jericho: Kenny Omega. What a dynamic performer. What a fantastic, tremendous performer. But he's not the best in the world. [tears up a A4 picture of Kenny Omega]I am. So many have claimed to be the best - from Shawn Michaels, to Edge, to CM Punk - but they're all gone. And I'm still here. Because I am the best in the world. I am the Greatest of All Time. I am the Alpha of this business... and I wanna prove it. 'Cause I'm challengin' you, Kenny. Jericho vs. Kenny. Alpha vs. Omega. To see who is really the best. I wanna meet you. [beat] January 4th. At Wrestle Kingdom 12 in the Tokyo Dome! So let's see who's really the best. Bitch.

Radio

The WNYC program Radio Lab episode Choice(501) contained an instance of this, subverting the line tagged onto the end of many radio broadcast introductions. "Stay with us, bitches."

One of Detroit radio personalities Dave & Chuck the Freak's catch phrases is "It's Friday, Bitches!" Actually, it's more than just a catch phrase - there's a short rap song with the title that was made just for them (which is played on the Friday shows, naturally), merchandise featuring the phrase, and even an annual event called the Friday Bitches Bash.

True Crime: Streets of L.A.. uses this liberally, but I guess the greatest example is when Nick Kang, the protagonist, finishes off the crime lord who had his father killed over a money laundering scheme. While this could be a bit of a solemn moment, as he watches his father's killer burn to death in a plane crash, he follows it up by saying: "Try Laundering THAT, bitch!"

Duke Nukem: Duke, despite his extreme womanizing, is a Chivalrous Pervert who never insults the objects of his desire. When you hear him confront the female monster Alien Queen and bellow, "I'm gonna kick your ass, bitch!", you know it is on.

Prince Bernard: How can this be happening? Felicia: Gender equality, bitch...

Web Animation

Tankmen 2: "You should've used the Pythagorean theorem, bitch."

The end of Neurotically Yours' "Caffeine Conspiracy" has Franklin the Star Shmucks peddler warn his superiors over walkie talkie that "The squirrel is on to us." A moment later, he starts another transmission to add "bitch."

Dinosaur Comics had a strip about the overuse of the word "bitches", and suggested alternatives like "fellows", "gravy", and "Monterey Jack". Possibly a Lampshade Hanging, since T-Rex is known for exclamations like "Phlogiston, bitches!" and "Live and learn, bitches!"

Most of the guest characters of The Angry Video Game Nerd ("Or do ya wanna play my game, bitch?"). And sometimes the Nerd himself ("Yeah, Super Mecha Death Christ 2,000 B.C. Version 4.0 Beta, bitch.").

slowbeef: I— maybe it was, like, how to get a refund... Like, "Don't be afraid to take back your pumpkins." Diabetus: This is an example of a defective product. Here's what you should do in this situation, bitch.

When Auto-Tune the Newssongified Christine O'Donnell's famous "I'm not a witch" campaign ad, they altered part of it into "I'm not a witch, bitch".

SMBC Theater: Combined with Sophisticated as Hell in Problem Solved. When newly elected U.S. President James Ashby is annoyed that his adviser keeps objecting to his ridiculous and irresponsible policy decisions, he says: "I'm sorry? Did you win a plurality of the Electoral College, bitch?"

Western Animation

Family Guy: In a Cutaway Gag, the MGM lion cub gets laughed at by his classmates when he wants to roar at an audience when he grows up. Soon, the lion is grown up and shouts "What's up now, bitches?!"

Moonbeam City: In Episode 7, at an "all-gang" meeting which includes the Moonbeam City Police Department (called "the roughest gang in town"), the city's top criminal gang leader repeatedly corrects himself when speaking to Police Chief (and millionaire heiress) Pizzaz Miller. His lines end with him referring to her as "bitch", which he repeatedly corrects to "ma'am". This includes his final line to her: "You have our word, bitch! I mean, ma'am..."

Brickleberry has the Ranger Games episode where Steve, desperate to beat the ringer Woody replaced him with on the gymnastics event, runs off to get some steroids, except he doesnt realize that you still have to work out, so he ends up massively overweight with excess body mass and a deep voice.

Fat!Steve: Wassup, bitches?!

Real Life

After carefully outlining the many, many reasons that global warming is real, a scientist famously ended her presentation with:

Richard Dawkins, at the end of a debate with an anti-scientific creationist gives us this gem: "If you base medicine on science, you cure people. If you base the design of planes on science, they fly. If you base the design of rockets on science, they reach the moon. It works...bitches." Also doubles as Sophisticated as Hell.

The cover of one birthday card involves one woman asking another "So where's your birthday at?" and the woman with the birthday tells her not to end a sentence with a preposition. On the inside, the first woman rephrases her question as "So where's your birthday at, bitch?"

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