cell adhesion assay - using collagen (Jun/16/2010 )

Hi,

I'm currently oerforming cell adhesion assays on my cell lines expressing my gene of interest vs cell line transfected with vector only. I'm using a colorimetric method. Howver, I do not understand the rationale behind this assay. If a cell line is tumor suppressive, do we expect it to have more cells adhering, i.e higher abosrbance reading comapred to the control or vice versa?

I thought if a cell line is tumor suppresisve, more cells would adhere, demonstrating reduced metastasis ability?

-SF_HK-

SF_HK on Jun 17 2010, 02:26 AM said:

Hi,

I'm currently oerforming cell adhesion assays on my cell lines expressing my gene of interest vs cell line transfected with vector only. I'm using a colorimetric method. Howver, I do not understand the rationale behind this assay. If a cell line is tumor suppressive, do we expect it to have more cells adhering, i.e higher abosrbance reading comapred to the control or vice versa?

I thought if a cell line is tumor suppresisve, more cells would adhere, demonstrating reduced metastasis ability?

this may be a feature of carcinoma or adenocarcinoma cells: Attachment gives survival signals which are less needed for carcinoma cells; perform also as a control experiment a poly-HEMA attachment assay