Tissue Staining

Life Technologies offers a variety of research use cell and tissue staining kits designed to identify target cells for laser capture microdissection using the Arcturus® LCM microgenomics system. These tissue preparation kits are used to stain tissues to aid in identification and sourcing of biomolecules for downstream analysis.

Obtain High-Quality RNA from Many Tissue Types The HistoGene® kit has been validated by examining RNA integrity on an expanding list of tissue types. All tissues tested to date using the HistoGene® kit have yielded high-quality RNA (Table 1).

Table 1. Tissues Validated with the HistoGene® Kit.

Foreskin

Ileum

Jejunum

Kidney

Liver

Brain

Salivary Gland

Thymus

Small Intestine

KIT0415,HIS0101,KIT0401,KIT0419

Arcturus® HistoGene® LCM Immunofluorescence Staining Kit

The Arcturus® HistoGene® LCM Immunofluorescence Staining Kit is designed to retrieve high-quality nucleic acids from immunofluorescently stained, frozen tissue using a quick 15-minute process. The kit provides reagents and slides for convenient and reliable immunofluorescent staining as well as protocols that are streamlined and optimized to maintain nucleic acid quality.

Brilliant High-Contrast Label Intensity The HistoGene® LCM Immunofluorescence Staining Kit employs a biotin–avidin system with Cy®3 dyes, resulting in exceptionally good staining intensity and specificity. The staining kit is used with a biotinylated monoclonal primary antibody against an antigen of choice provided by the user, and Cy®3 conjugated streptavidin is included in the kit (Figures 2).

Obtain High-Quality RNA from Many Tissue Types The HistoGene® LCM Immunofluorescence Staining Kit has been validated by examining RNA integrity on an expanding list of tissue types. All tissues tested to date using this kit have yielded quality RNA (Table 2).