Recognizes a protein of 62kD, identified as cdc6. The replication licensing system acts to ensure that no section of the genome is replicated more than once in a single cell cycle. The origin recognition complex (ORC) and cdc6/cdc18 are needed on chromatin before the licensing reaction can take place. During the transition from a growth-arrested to a proliferative state, transcription of mammalian cdc6 is regulated by the cell cycle transcription factor (E2F protein). Immunoblots show that quiescent cells lack cdc6 and that minichromosome maintenance (MCM) proteins are not associated with chromatin. Competence of G1 phase nuclei to replicate in vitro coincides with maximum cdc6 accumulation and MCM protein binding to chromatin in vivo. Antibodies against cdc6 and MCM5 stain abnormal (neoplastically transformed) cells in cervical smears and sections with remarkably high specificity and sensitivity. Thus antibodies against proteins that regulate DNA replication may reduce the high false-negative rate of the Pap smear test and may facilitate mass automated screening.

Catalog #

C2550-05X

Hybridoma

BALB/c splenocytes and mouse myeloma NS-2 cells.

Applications

Immunoprecipitation, Western Blotting. Not assessed for Immunohistochemistry.

Recommended Dilution

Immunoprecipitation: Use Protein G; 2ug antibody/mg protein lysate

Western Blotting: 1ug/ml

Optimal dilutions to be determined by the researcher.

Positive Control

Raji cells. Tonsil or lymph node.

Cellular Localization

Nuclear

Storage and Stability

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Clone Type

Monoclonal

Isotype

IgG1

Clone No

0.N.189

Host

Mouse

Source

Human

Concentration

1mg/ml

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. Also available with BSA and azide. See C2550-05.

Purity

Purified by Protein G affinity chromatography.

Immunogen

Recombinant human cdc6 protein.

Specificity

Recognizes human cdc6 at 62kD. Species Reactivity: Human.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.