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Predicted species reactivity based on immunogen sequence: Rabbit: (87%)

IMMUNOGEN:

JPH1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human JPH1.

The immunogen is located within amino acids 470 - 520 of JPH1.

TESTED APPLICATIONS:

ELISA, IHC-P, WB

APPLICATIONS:

JPH1 antibody can be used for detection of JPH1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.

POSITIVE CONTROL:

1) Cat. No. 1210 - HEK293 Cell Lysate

Properties

PURIFICATION:

JPH1 Antibody is affinity chromatography purified via peptide column.

CLONALITY:

Polyclonal

ISOTYPE:

IgG

CONJUGATE:

Unconjugated

PHYSICAL STATE:

Liquid

BUFFER:

JPH1 Antibody is supplied in PBS containing 0.02% sodium azide.

CONCENTRATION:

1 mg/mL

STORAGE CONDITIONS:

JPH1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL:

JPH1

ALTERNATE NAMES:

JPH1 Antibody: JP1, JP-1, JP1, Junctophilin-1, Junctophilin type 1

ACCESSION NO.:

AAI39833

PROTEIN GI NO.:

145337941

GENE ID:

56704

USER NOTE:

Optimal dilutions for each application to be determined by the researcher.

Background and References

BACKGROUND:

JPH1 Antibody: Junctional complexes between the plasma membrane (PM) and endoplasmic/sarcoplasmic reticulum (ER/SR) are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. Junctophilins (JPs) are important components of the junctional complexes. JPs are composed of a carboxy-terminal hydrophobic segment spanning the ER/SR membrane and a remaining cytoplasmic domain that shows specific affinity for the PM. Four JPs have been identified as tissue-specific subtypes derived from different genes: JPH1 is expressed in skeletal muscle, JPH2 is detected throughout all muscle cell types, and JPH3 and JPH4 are predominantly expressed in the brain and contribute to the subsurface cistern formation in neurons. JPH1 is essential for stabilizing the T-tubule and SR membranes to form junctions and provide an environment for the assembly of receptors such as the ryanodine receptor type 1 (RyR1).