Have any scientific studies been conducted to determine the effects of these chemicals on hummingbirds?

Some people are surprised to learn that the answer is an emphatic “NO.” Despite oft-repeated (and oft-debunked) urban legends that the San Diego Zoo, Audubon Society, or some other trustworthy source tested red dye on hummingbirds and found one or more specific effects (liver damage, kidney damage, cancer, tumors, “birth defects,” weakened eggshells, or, in some versions of the story, no harm at all), there is no evidence that any such testing has ever been conducted on hummingbirds by anyone anywhere.

Considering the challenges of such a study, direct testing of dyes on hummingbirds is not likely to happen:

Hummingbirds, like most wild birds, are protected by the Migratory Bird Treaty Act and various state laws, and animal research is subject to its own set of federal, state, and institutional regulations, so the first hurdle would be to get all the necessary permits and permissions to capture wild hummingbirds, confine them at a research facility, and perform lethal testing on them.

Getting the permits would require the researchers to prove that they could provide proper housing and care for the duration of the study. Hummingbirds are difficult and expensive to house and feed, and it could cost many thousands of dollars to keep dozens of them alive and otherwise healthy for the duration of testing.

The size and composition of the study population is very important. If the number of birds is too small, the results may not be clear, or critics may dismiss them as not statistically significant. If only one species is tested, critics may argue that the results may not apply to other hummingbirds. If only males are tested, critics may contend that the dye could affect females differently or not at all and therefore have little effect on populations.

To document the effects of dye consumption on the birds, the researchers would have to “sacrifice” and dissect them. It would be difficult to find qualified researchers interested enough in hummingbirds to perform such a study, qualified to undertake or supervise daily care of dozens of captives, and willing to kill them all to get the data. Imagine, too, how the hummingbird-loving public would react to learning that dozens of hummingbirds had been taken from the wild, dosed with dyes, killed, and dissected, even if the results might help to protect the health and safety of millions of other hummingbirds.

“Skeptics” who insist that such studies are required to “prove” that petroleum-based artificial dyes harm hummingbirds are setting the bar far higher than we do for our own health. No one in their right mind would suggest that we evaluate the safety of food additives, drugs, etc. by exposing human subjects to potentially harmful doses. Instead, we rely on the results of testing on lab animals and cell cultures to indicate whether and how various chemicals may affect human health.

Just because artificial dyes have never been tested on hummingbirds doesn’t mean they’ve never been tested. In fact, there are plenty of published studies on the effects of FD&C Red No. 40 and FD&C Red No. 3 to help us make a compelling case against exposing hummingbirds to high doses of these dyes, without taking the life of a single bird.* The real challenges are to increase awareness of this evidence among backyard enthusiasts and persuade manufacturers of commercial “instant nectar” products to use safer alternatives.