RE: [Histonet] AE1/AE3 cross reactivity

From:

"Sally Price"

Dr. Hessler:
I have observed the phenomenon that you've described for other antibodies
more times than I can remember. Personally, I believe that it has to do
with the way the Ventana system sprays on the retrieval solution
and heats slides during antibody/detection incubation periods, which is too
aggressive. I think that an open system that doesn't heat slides or perform
AR isn't likely to produce these unexpalined reactions, but I digress. So,
how do you fix the problem? I say disable the on-line AR, and perform it in
a decloaking chamber, set at 100 degrees for 10 minutes. Or, you could
consider using another pan-CK antibody, like Lu-5.
Good Luck,
Sally Price
----------------------------------------------------------------------------------------------
Date: Wed, 16 Jan 2008 07:57:41 -0600
From: "Richard Hessler, M.D."
Subject: [Histonet] AE1/AE3 cross reactivity
To: "histonet@lists.utsouthwestern.edu"
"
I have used this antibody for years but now have issues. Previously it was
the best choice for breast sentinel nodes, but now AE1/AE3 stains plasma
cells like CD138. It also stains a number of different sarcomas and
beautifully highlights reactive astrocytes. I had previously used Dako Ab
and autostainer with no problems. Now I have Ventanna and have noticed this
with both Ventana and Dako Abs used on that system.
Any thoughts???
Richard B Hessler, MD
Chief of Pathology
Erlanger Medical Center
Chattanooga, TN
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