I minprepped pBEST-Pr-MGapt-UTR1-deGFP-T500 from last weeks frozen tubes of midi-culture using the Sigma kit.

I reduced volume from 1.2 mL to ~100 μL by spinvac, and then I purified the DNA by PCR Purification Kit.

I verified the plasmid DNA by DD with SphI and NcoI, which was expected to yield 3102 bp and 139 bp dsDNAs.

Gel electrophoresis showed a band at ~3000 bp, though the 139 bp band was not visible on this gel (neither was the 100 bp ladder). Uncut plasmid DNA traveled farther, and there were two higher order plasmid states, which disappeared in the cut DNA, as expected.