The 11th International Symposium on Therapeutic Ultrasound (ISTU 2011), New York, NY., 11-13 April 2011. In Final Program and Abstracts Book of ISTU 2011, 2011, p. 108 How to Cite?

Abstract

Even after committing to osteolineage, human meschenchymal stem cells (hMSCs) tend to develop into osteoblasts at a slow pace that may not lead to significant bone mineral deposits after weeks. In attempt to accelerate this process, we have investigated how hMSCs can be stimulated towards osteogenesis in-vitro through low-intensity pulsed ultrasound (LIPUS) exposures. The hMSCs (500000cells/ml) were seeded within a collagen meshwork that served as an osteo-conductive platform and were cultured in the presence of osteolineage supplements. Each experimental group (N>3) was treated with LIPUS for seven consecutive days (frequency: 1MHz; duty cycle: 20%; PRF: 1kHz; duration: 30min; peak-negative pressure: 0, 60, 80, 90, 105, 120, 180kPa), and in one group 1% microbubbles was added before the daily LIPUS expsoure. Results show that: 1) LIPUS did not affect the seven-day viability of hMSCs (confirmed by fluorescent live-dead assay); 2) LIPUS has transiently increased the secretion of the BMP-2 protein that is involved in the osteoinduction process; 3) LIPUS has led to increase in the seven-day calcium deposit level, with maximum detected at 60-120mW/cm2 intensities; 4) observations were similar for the group with microbubbles, and thus stable cavitation may not be a contributing factor to osteogenesis.

The 11th International Symposium on Therapeutic Ultrasound (ISTU 2011), New York, NY., 11-13 April 2011. In Final Program and Abstracts Book of ISTU 2011, 2011, p. 108

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http://hdl.handle.net/10722/140314

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Nominated Abstracts for the 2011 ISTU Student Competition: no. 18

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Session 2K-Gene/cell therapy: 1569403433

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Final Program can be download at: http://www.istu.org/events/ann2011/finalProgram.pdf

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dc.description.abstract

Even after committing to osteolineage, human meschenchymal stem cells (hMSCs) tend to develop into osteoblasts at a slow pace that may not lead to significant bone mineral deposits after weeks. In attempt to accelerate this process, we have investigated how hMSCs can be stimulated towards osteogenesis in-vitro through low-intensity pulsed ultrasound (LIPUS) exposures. The hMSCs (500000cells/ml) were seeded within a collagen meshwork that served as an osteo-conductive platform and were cultured in the presence of osteolineage supplements. Each experimental group (N>3) was treated with LIPUS for seven consecutive days (frequency: 1MHz; duty cycle: 20%; PRF: 1kHz; duration: 30min; peak-negative pressure: 0, 60, 80, 90, 105, 120, 180kPa), and in one group 1% microbubbles was added before the daily LIPUS expsoure. Results show that: 1) LIPUS did not affect the seven-day viability of hMSCs (confirmed by fluorescent live-dead assay); 2) LIPUS has transiently increased the secretion of the BMP-2 protein that is involved in the osteoinduction process; 3) LIPUS has led to increase in the seven-day calcium deposit level, with maximum detected at 60-120mW/cm2 intensities; 4) observations were similar for the group with microbubbles, and thus stable cavitation may not be a contributing factor to osteogenesis.