Huygens Re-scan Confocal Optical Option

Since Huygens version 18.10, SVI also offers a specialized Huygens deconvolution option to optimally process Re-scan Confocal Microscopy (RCM) images. The Huygens Re-scan Optical Option can be added to a Huygens license as a seperate option, and improves acquired RCM images signficiantly.

Images show individual chromosomes of a nuclear spread from fixed mouse spermatocytes, immunostained for SYCP3 a component of the synaptonemal complex (Alexa 488-labelling). Raw images were acquired with RCM without re-scanning (confocal) and with re-scanning (RCM). The raw RCM image was deconvolved with the Huygens Re-scan Optical Option.
Sample courtesy of A. Agostinho – Advanced Light Microscopy Facility, Science for Life Laboratory and C. Höög – Department of Cell and Molecular Biology, Karolinska Institutet (Sweden). Imaging and Huygens deconvolution was performed by Erik Manders (Confocal.nl).

About the Re-scan Confocal Microscopy system

The RCM system is an affordable add-on system developed and sold by Confocal.nl. This system can turn any fluorescent microscope into a 3D confocal system. The commercial add-on system is based on the Re-scan paper published by De Luca et. al (1). For more information on the RCM system, see the Confocal.nl website.