Exocellulase CBH I – Purified Enzyme

A Recombinant Cellobiohydrolase (commonly called an Exocellulase) from Hypocrea Jecorina

Infinite Enzymes CBH I is an exocellulase (Cel6A: E.C.3.2.1.91) from Hypocrea jecorina. It is produced from a recombinant corn seed production system. It will be provided as an ammonium sulfate precipitate. Activity is determined using methyl-umbelliferyl beta-D cellobioside (MUC).

Cellobiohydrolase is an enzyme present in many fungi, but particularly wood rot fungi. It is a monomer of 59 kDa with a catalytic domain and a cellulose binding domain. The reaction adds water to the glucose bonds in cellulose (non-reducing ends of the chain), yielding cellobiose:

APPLICATION

Infinite Enzymes’ Cellobiohydrolase can be used in combination with Endocellulase and β-glucosidase to produce glucose from cellulose. Its activity is dependent on several factors including inhibitors in the substrate (pure cellulose versus plant-derived cellulose), enzyme dose, concentration of other enzymes, feedback inhibition of products, temperature and pH.

EFFECT OF TEMPERATURE AND pH ON ACTIVITY

Optimal temperature for this enzyme is approximately 45° C, although it functions from 37°C through 50°C. pH optimum is 5.0 – 6,0.

The enzyme is produced from recombinant maize that is grown under compliance with USDA regulatory guidelines. Unlike other commercially available cellulase enzyme, purified CBH I is being produced without typical impurities (other enzymatic side activities) found in fungal-produced enzymes.