the receptor pentapeptide-binding site is located at the juncture between the carboxyl-terminal end of alpha-5, the final piece of secondary structure in the regulatory domain, and the linker that connects the regulatory and catalytic domains

the Thermotoga maritima CheB methylesterase domain has identical topology of a modified doubly-wound alpha/beta fold, but the CheB demethylation consensus sites of the chemoreceptors, the CheB substrate, are not uniquely conserved

phosphorylation by phosphoramidate requires Mg2+, phosphorylation of the regulatory domain at Asp-56 results in a reorganization of the domain interface, allowing exposure of the active site to the receptor substrate and simultaneously stimulating methylesterase activity

In its unphosphorylated state, the regulatory domain inhibits enzyme activity of effector domain. Phosphorylation of the regulator domain leads to an enhancement of enzyme activity through a relief of inhibition and a stimulatory effect on catalysis.

In its unphosphorylated state, the regulatory domain inhibits enzyme activity of effector domain. Phosphorylation of the regulator domain leads to an enhancement of enzyme activity through a relief of inhibition and a stimulatory effect on catalysis.