CBpromise

shRNA Clones

Knockdown of gene expression at the mRNA level by RNA interference (RNAi) is a common method for investigating gene function. For transient knockdown in mammalian cell culture, small interfering RNA (siRNA) is often favored. The advantages of siRNA include commercially available RNA oligos that can be transfected into cells for quick and efficient knockdown. However, siRNA becomes less useful when working with cell types with low transfection efficiency or in experiments that require prolonged gene knockdown. Another common method for utilizing RNAi is short-hairpin RNA (shRNA), synthetic non-coding RNA which utilizes the endogenous microRNA machinery to process functional RNAi.

shRNA is a sequence of RNA which makes a tight hairpin turn that can be used to silence target gene expression via RNAi. Expression of shRNA in cells is typically accomplished by delivery of plasmids or through viral or bacterial vectors. Compared to siRNA, shRNA offers benefits in silencing longevity, delivery options and cost. Expressed shRNA is transcribed in cells from a DNA template as a single-stranded RNA (ssRNA) molecule (~50 – 100 bases). Complementary regions spaced by a small 'loop' cause the transcript to fold back on itself forming a 'short hairpin' in a manner analogous to natural microRNA. Recognition and processing via the RNAi machinery converts the shRNA into the corresponding siRNA.

Creative Biogene supplies a series of shRNAs, which could make tight hairpins used for silencing target gene expression via RNAi. Our comprehensive shRNA product offering currently consists of approximately 200,000 pre-cloned shRNA constructs. Each gene is represented by multiple vector constructs targeting different regions of the gene sequence. Each shRNA is cloned into the plasmids or lentiviral vector and each construct is sequence verified to ensure a match to the target gene.

Please browse our shRNA clones collection to find your desired one. If you don’t find the shRNA clones of your interest in our collection, we can also work for your project with the best price!