Evaporation Insights

Production Lyo Center

Thermal Discovery Center

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View recent webinars on "Techniques for Enhancing Visualization of Lyophilization Cakes"; the three-part series on "Lyophilization Validation: Operational Qualification and Process Validation"; learn the "Basic Theory of Freeze Drying" and much more...

Support

Our people make the difference.

SP Scientific service professionals offer your organization a depth of expertise that is truly unique to our selected industries and markets. We are recognized experts in applications, methodologies, lab processes and many other areas that directly impact your day-to-day operations. With SP Scientific your solution is just a call or an e-mail away.

SP Scientific service professionals are highly knowledgeable and are qualified to support every instrument, software and application product that SP Scientific has brought to the market.

DNA & Oligonucleotides

Genevac systems facilitate a wide range of DNA
sample preparation and processing activities, ranging from
straightforward small volume concentration of DNA pellets prior to PCR,
through to high throughput processing of many purified DNA or
oligonucleotide samples. Where a dry sample of DNA is required,
concentration to dryness in a centrifugal evaporator is the ideal
method, presenting the DNA as a dry film in the tube of plate well.
Freeze drying of DNA samples is not recommended because the resultant
DNA powder is easily blown about or moved by electrostatic charges.

DNA ConcentrationConcentration of DNA from
samples containing water or a mixture of water and alcohols is a simple
process, which for most researchers requires the miVac DNA concentrator.
Concentration to remove a few hundred microlitres of alcohol from a DNA
pellet takes approximately 10 minutes, or less. This is one of the
simplest applications of a Genevac system – in a miVac DNA concentrator,
set the temperature, select the method for alcohols or water, and press
start.

Oligonucleotides & DNA PurificationThe
manufacture of oligonucleotides commonly has two steps where evaporation
is required, evaporation of ammonia solutions following synthesis, and
then subsequent evaporation of purified samples. Protection of
oligonucleotides from damage during drying is critical, especially where
a tag or label has been attached to the DNA. Two studies by Genevac
users show that this can safely be achieved in a Genevac evaporator:

Samples containing ammonia solution should be evaporated using Dri-Pure® (500g)
to prevent sample loss and cross contamination. When ammonia solution
is subjected to a vacuum the ammonia degasses. Degassing must be
controlled or the resultant foam overflows the tube causing sample loss
and cross contamination. In the same way that you control degassing of a
bottle of soda by opening it slowly, gentle application of vacuum is
required, this coupled with the high rotors speeds of Dri-Pure keep every sample where it should be. The latest versions of the EZ-2 come with such a method pre-programmed, and the method is simple to program on the HT-Series.

Where samples have been purified by reverse phase HPLC samples are
presented in a mixture of water and methanol or acetonitrile. The
methods laid out in the Post Purification Sample Handling section should be followed. The imbalance tolerance built into Genevac systems gives researchers confidence that eluates at
different ends of the gradient will still dry down, and if there is too
much imbalance the system will automatically shut down and tell the
user.

Preparation of DNA MicroarraysFor certain microarraying techniques concentrated samples of DNA are required. To maintain such a
concentration is difficult due to the volatile nature of the buffers
holding the DNA, therefore samples should be made up to the required
volume immediately prior to analysis. A study by the Wellcome Trust Centre for Human Genetics in Oxford has shown that when an EZ-2 is used in this process the data quality returned by the system is significantly better, reducing the no call rate by 80% or
more.

Call us toll free: 800-431-8232 or 845-255-5000

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Company Overview

SP Scientific is the synergistic collection of well-known and highly regarded scientific equipment brands, including VirTis, FTS Systems, Hull, Genevac, Hotpack, and most recently PennTech. The joining of these well-established brands as SP Scientific has created one of the largest and most experienced companies in freeze drying (lyophilization), centrifugal evaporation and concentration, temperature control/thermal management, glassware washers, controlled environments, vial washing and tray loading machines.

More than a scientific equipment supplier, SP Scientific represents brands that distinguish themselves by being able to more thoroughly assist customers in matching equipment to particular application needs. Fortune 500, pharmaceutical, aerospace, automotive, medical device, diagnostic kit and biotechnology companies — as well as government facilities, universities and colleges are among the organizations served on a daily basis.

SP Scientific is constantly striving for superior service and quality. We want to ensure that our customers become part of our “family” and that we serve each customer to the best of our abilities. Each manufacturing facility is an ISO 9001:2008 rated facility. Industries such as pharmaceutical manufacturing have required that the associates at SP Scientific are well versed in current Good Manufacturing Practices (cGMP), Good Automated Manufacturing Practices (GAMP5), 21CFR, Part 11, electronic signatures and ASME standards.

SP Scientific is a global company with manufacturing facilities in Gardiner and Stone Ridge, New York; Warminster, Pennsylvania; and Ipswich, United Kingdom. Regional sales associates, Distributors and service technicians are located throughout the world. SP Scientific associates and distributors look forward to helping you reach your goals.

SP Scientific is part of SP Industries, Inc.,
a leading designer and manufacturer of state-of-the-art laboratory
equipment, pharmaceutical manufacturing solutions, laboratory supplies
and instruments, and specialty glassware. SP's products support research
and production across divers end user markets including pharmaceutical,
scientific research, industrial, aeronautic, semiconductor and
healthcare. SP's three flagship brands -- SP Scientific, SP Scienceware, and SP Ableware
-- represent over 400 combined years of experience, quality, and
innovation. In December, 2015, SP Industries was acquired by Harbour Group,
a private investment firm founded in 1976. Harbour Group is a privately
owned, operations focused company based in St. Louis, Missouri. Headquartered in Warminster, Pennsylvania, SP Industries has
production facilities in the USA and Europe and offers a world-wide
sales and service network with full product support including training
and technical assistance.