A Continuous-Flow Microdroplets Lysis System [BPN702]This Project aims for developing a versatile continuous-flow system for lysing microdroplet. Microdroplets have been widely
utilized in diverse chemical and biological research and applications such as DNA sequencing or nanoparticle synthesis. Although
highly robust and easy handling techniques of droplets are essential for those purposes, difficulties still remain in retrieving
inner contents (e.g. cells, microbeads, biomolecules, reagents) of droplets for further experiments. Here we present a novel
microfluidic system to achieve three distinctive accomplishments: (i) guiding droplets between different phase flows, oil flow and
water flow, (ii) rapid washing process of surfactant on droplets, and (iii) quick and easy lysing of droplets and releasing their
contents into water flow. The presented system offers a high-throughput method to wash and water-in-oil droplets for
expanding droplet-based applications.