absorption coefficients

Kai Schmengler schrieb:
>> Dear all!
>> I determined Km-values of an enzyme with the substrates guaiacol and
> potassium iodide. Unfortunately I chose wavelengths (near published
> ones) I didnt have the absorption coefficients (extinction
> coefficients) for. Now Im looking for a table or papers in which those
> coefficients could be published. The wavelenghts were 405 nm for
> potassium iodide and 450 nm for guaiacol.
If you know literature values at some wavelengths, why don't you just
measure accurate spectra and calculate the absorption coefficients at
the desired wavelengths?
Frank
--
Die Verwendung von mehreren Ausrufezeichen macht die Aussage nicht
ausrufender sondern ausufernder. [Michael Bauer in dnq]