Education - Chromosomes - FISH

Chromosome FISH

(Fluorescence in situ hybridization)

The analysis of pachytene bivalents was possible but not easy. To somatic chromosomes, G-banding and C-banding techniques used to many other organisms were not applicable because they were too small. Since the latter half of 1980s, Fukui et al. tried graphic analysis in order to overcome this difficulty. Then in the 1990s, FISH was applied to the construction of the physical map of chromosomes (Figure 5) and also to the differential staining of the chromosomes (chromosome painting) from different species.

Figure 5. Locating YAC clones to the first and fourth chromosomes by FISH.
(This figure is originated from Ohmido et al.)