Abstract

Objective: The aim of this study was to determine the effect of low-energy He-Ne laser treatment on wound
healing dynamics (histological and biochemical) in diabetic rats. Background data: Low-energy laser photostimulation
at certain wavelengths can enhance tissue repair by releasing growth factors from fibroblasts and can facilitate the healing process of diabetic wounds. Materials and methods: A circular 4 cm2 excisional
wound was created on the dorsum of the experimentally (Alloxan)-induced diabetic rats. In the study group
(N = 24) the wound was treated with He-Ne laser (632.8 nm wavelength) at a dose of 4.8 J/cm2 for 5 days a
week until the wound healed completely. The control group (N = 24) was sham-irradiated. The results were
statistically analyzed by an independent t test for biochemical analysis and the nonparametric Mann-Whitney
U test for histopathological parameters. Results: The analysis of the biochemical parameters and histopathological
parameters of the wounds showed that the laser-treated group healed faster and better as compared to
the control group (p < 0.0001). The laser-treated group healed on average by the 18th day whereas, the control
group healed on average by the 59th day. Conclusion: Laser photostimulation promotes the tissue repair process
of diabetic wounds.