this is Ros. I’m having troubles with an image (z-stack) that I didn’t collect and when I open it in Fiji, it turns out to be in inches. I tried to ask for the micron / pixel and the only info I have at the moment are the following that derive from the photo itself:
slice number
pixel width 0.0033333
pixel height 0.0033333
Volxel depth 1.0
Frame interval 0 sec
Origin 0.0
At the end the photo is large 5.33 x 4 inches that I don’t think are real measurements considering that they correspond to about 12 x 10 cm … since we are talking about dendrites, I don’t find it possible.

What I know I would like the software to know is that:
Images are acquired every 1 micron
voxel size is 0.185 um

I tried to convert these micron to inches and to replace at least these two values, but it doesn’t change the detail of the image’s size. Moreover, every time I do this process, the Simple Neurite Tracer tool no longer works well and tracks unreal things.
Can you help me?

I am sorry, but I am not allowed to share the image. Anyway, attached you can find the screedshot containing the original image properties.

I guess that the image has the calibration of the microscope from witch it derives. But, even if I have inches in the original format, I was trying to assigne it the voxel and the z-step in Analyse -> Set scale; it is not collaborating. And just to add one more problem, when I try this procedure the Neuron traces doesn’t perform as it should.

sorry I gave you no update, but I tried and tried again without success. I was able to get the authorization to send you an image with which I have not only the problem of scale, but also the problem of not being able to perform 3D reconstruction. Can I still attach the image?

thanks for this advice. I did it and actually it works. One less problem!!!

Unfortunatelly, the measurement are not accurate at all. The point is that, regarding these images, I have to be able to tell the program that the images are acquired every 1 micron (z-stacks) and that the voxel size is 0.185 um. I have to trace that neuron and measure the lenght of its branches (I am doing it with neurona traces tool). As it is now set, my images says that my neurons are large cm, when it is impossible. How can procede about giving it the real scale?
Is this info (1 micron (z-stacks) and that the voxel size is 0.185 um) enough for me or do I need anything else? In Analysis - Tool- Set scale, for example, it doesn’t let me put voxels.

Please, let me know if you need any other information to help me get to the solution.