Dawson, IL

George J. Dawson, Libertyville, IL US

Patent application number

Description

Published

20090068635

INDIRECTLY LABELLED ASSAY CONJUGATES AND METHODS OF PREPARING AND USING SAME - Indirectly labelled assay conjugates prepared by a method that includes the step of submitting the binding member comprised by the conjugate to denaturing conditions prior to labelling the binding member. The indirectly labelled assay conjugates demonstrate an increased sensitivity when employed in diagnostic assays compared to assay conjugates prepared by methods that do not include a step of submitting the binding member to denaturing conditions prior to labelling. Processes for the preparation of the indirectly labelled assay conjugates, methods of detecting an analyte comprising the use of the indirectly labelled assay conjugate and kits comprising the indirectly labelled conjugates are also provided.

INDIRECTLY LABELLED ASSAY CONJUGATES AND METHODS OF PREPARING AND USING SAME - Indirectly labelled assay conjugates prepared by a method that includes the step of submitting the binding member comprised by the conjugate to denaturing conditions prior to labelling the binding member. The indirectly labelled assay conjugates demonstrate an increased sensitivity when employed in diagnostic assays compared to assay conjugates prepared by methods that do not include a step of submitting the binding member to denaturing conditions prior to labelling. Processes for the preparation of the indirectly labelled assay conjugates, methods of detecting an analyte comprising the use of the indirectly labelled assay conjugate and kits comprising the indirectly labelled conjugates are also provided.

MATERIALS AND METHODS FOR ASSAY OF ANTI-HEPATITIS C VIRUS (HCV) ANTIBODIES - A polypeptide comprising the contiguous amino acids 1-198 of SEQ ID NO: 2; a polypeptide, which comprises a contiguous amino acid sequence that is at least about 95% identical to the contiguous amino acids 1-198 of SEQ ID NO: 2, an epitope that is immunoreactive with an antibody that specifically binds to the core protein of hepatitis C virus (HCV), and an epitope that is immunoreactive with an antibody that specifically binds to the NS4 region of HCV; a nucleic acid encoding such a polypeptide; a host cell comprising such a nucleic acid; an immunodiagnostic reagent comprising such a polypeptide; a kit comprising such an immunodiagnostic reagent; and a method of determining the presence, amount, or concentration of anti-HCV antibodies in a test sample.

08-09-2012

20120329176

INDIRECTLY LABELLED ASSAY CONJUGATES AND METHODS OF PREPARING AND USING SAME - Indirectly labelled assay conjugates prepared by a method that includes the step of submitting the binding member comprised by the conjugate to denaturing conditions prior to labelling the binding member. The indirectly labelled assay conjugates demonstrate an increased sensitivity when employed in diagnostic assays compared to assay conjugates prepared by methods that do not include a step of submitting the binding member to denaturing conditions prior to labelling. Processes for the preparation of the indirectly labelled assay conjugates, methods of detecting an analyte comprising the use of the indirectly labelled assay conjugate and kits comprising the indirectly labelled conjugates are also provided.

12-27-2012

Patent applications by George J. Dawson, Libertyville, IL US

George J. Dawson, Abbott Park, IL US

Glyn Dawson, Chicago, IL US

Patent application number

Description

Published

20110097797

Modular Functional Peptides for the Intracellular Delivery of Nanoparticles - Described are peptides for delivery of a nanoparticle to the cytosol, the peptide comprising: (a) a nanoparticle association domain; (b) a proline-rich spacer domain; (c) an uptake domain; and (d) a vesicle escape domain comprising a non-hydrolyzable lipid moiety, wherein the spacer domain is between the nanoparticle association domain and the uptake and vesicle escape domains, and wherein the peptide, when attached to an extracellular nanoparticle, is effective to induce uptake of the nanoparticle by a cell and delivery of the nanoparticle to the cytosol of the cell. Also described are methods of delivery of a nanoparticle to the cytosol of a cell, the method comprising providing to a cell a nanoparticle attached to such a peptide. Exemplary nanoparticles include quantum dots.

Modular Functional Peptides for Delivery of Nanoparticles - A peptide directs nanoparticles (such as quantum dots) to the plasma membrane of mammalian cells. A method of delivery of a nanoparticle to a plasma membrane of a cell includes providing to the cell a nanoparticle attached to a peptide configured to direct the nanoparticle the plasma membrane, and allowing the cell to take up the nanoparticle. The nanoparticle can be a FRET donor to an organic dye.

05-23-2013

20130158244

Modular Functional Peptides for the Intracellular Delivery of Nanoparticles - Described are nucleic acids encoding a polypeptide for delivery of a nanoparticle to the cytosol, the peptide comprising: (a) a nanoparticle association domain, (b) a spacer domain, (c) an uptake domain, and (d) a vesicle escape domain, wherein the domains (a) through (d) appear in the same order as listed above, and wherein the peptide, upon addition of a non-hydrolyzable lipophilic moiety to the vesicle escape domain and binding to a nanoparticle, is effective to induce uptake of a nanoparticle by a cell and delivery of the nanoparticle to the cytosol of the cell. Also described are methods of delivery of a nanoparticle to the cytosol of a cell, the method comprising providing to a cell a nanoparticle attached to such a peptide. Exemplary nanoparticles include quantum dots.

06-20-2013

John Caleb Dawson, Chicago, IL US

Patent application number

Description

Published

20100168862

Expandable Intervertebral Spacers and Methods of Use - An intervertebral spacer is inserted between vertebral members in a compact configuration and expanded post-insertion to contact the adjacent vertebral members. The intervertebral spacer comprises a first member and a second member movable with respect to the first member between retracted and extended positions.

07-01-2010

20100198352

Implant with Nested Members and Methods of Use - Vertebral implants that may be movable between collapsed and expanded orientations to space apart first and second vertebral members. The implants may include first and second contact surfaces to contact against the vertebral members. The implants may include nested struts. The implants may have a first height measured between the contact surfaces in the collapsed orientation with a first amount of overlap in the struts. The implants may have a second height in the expanded orientation with a lesser amount of overlap in the struts.

08-05-2010

20120296430

EXPANDABLE INTERVERTEBRAL SPACERS AND METHODS OF USE - An intervertebral spacer is inserted between vertebral members in a compact configuration and expanded post-insertion to contact the adjacent vertebral members. The intervertebral spacer comprises a first member and a second member movable with respect to the first member between retracted and extended positions.

TIE-DOWN ANCHOR FOR FREIGHT - A floor tie-down anchor for securing freight during transport is provided. A tie-down anchor is provided comprising an elongated L-shaped bracket including a first leg and a second leg forming an angle therebetween. At least one aperture having a first shape is defined through the first leg and configured to receive a connecting mechanism for securing freight. At least one aperture having a second shape different from the first shape is defined through said first leg. The first shape is an oval slot and the second shape is a circle, and the L-shaped bracket is operatively connected to a railcar having a floor such that the first leg is substantially coplanar with and parallel to the floor of the railcar.

05-13-2010

20100180516

TOP CHORD STIFFENER FOR ENCLOSED RAILCAR - A superstructure for a railcar comprising at least a first side assembly, a roof extending from the first side assembly and being comprised of at least a first top chord extending inwardly and upwardly from the first side assembly and a first top chord stiffener comprised of at least a first leg and a second leg. The first and second legs are disposed at a right angle to each other, and the first top chord stiffener is attached to the first top chord and the first side assembly.