This kit is designed to knockout or alter genes on the E.coli chromosome in less than one week. Red/ET recombination allows the exchange of genetic information in a base pair precise, specific, and faithful manner. An FRT-flanked kanamycin resistance marker cassette is supplied with the kit which can be used to replace a gene on the E.coli chromosome. Red/ET recombination can replace fragments as large as 30kb from the E.coli chromosome. The use of an FRT-flanked resistance cassette for the replacement of the targeted gene allows the subsequent removal of the selection marker by a Flp recombinase step, if required. Flp expression plasmids can be purchased from Gene Bridges. Multiple knockouts can be generated either by a repetitive insertion of the functional cassette supplied with the kit or by combination with other functional cassettes offered by Gene Bridges. The kit is available to academic researchers. Commercial organizations require a sub-license from Gene Bridges.

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