The essential oils of aerial parts of six Artemisia samples growing in Croatia (A. alba, A. annua (two samples), A. maritima, A. verlotiorum i A. vulgaris) were isolated by hydrodistillation. The oil yield varied between 0.4-0.9 (v/w). Chemical composition of the obtained essential oils were analysed by gas chromatography-mass spectrometry (GC-MS). A total of 87 compounds were identified. Large differences between samples were obtained in qualitative and quantitative composition. Even two samples of A. annua collected on different locations varied significantly in composition. Oil collected in Zagreb was characterised by high amounts of artemisia ketone (46.0%), artemisia alcohol (1.3%), camphor (18.7%) and β-selinene (8.8%). Oil collected in middle Velebit area contained higher amount of camphor (31.5%) and β-selinene (24.6%) and the highest amount of sesquiterpenes (30.7%) among all analysed samples but contained smaller amount of artemisia ketone (1.9%), artemisia alcohol (0.1%). A. vulgaris had similar major compounds as A. annua collected middle Velebit area having the highest amount of monoterpene oxides (80.8%) while A. verlotiorum had the highest amount of thujones. A. meritima had the highest amount of sesqterpene oxides (22.4%) while A. alba showed the highest qualitative difference among samples. The most of unidentified compounds belongs to oxygen containing sesquiterpene compounds which were contained in all analysed samples and which are difficult to identify.