Cayman Chemical Contact

Analysis Tools

Cayman offers a growing collection of useful analysis tools.
These tools are in the form of Microsoft Excel spreadsheet documents.
All are freely available for download. See the enclosed (liberal) license agreement for terms of use.

You can also find analysis tools for select Cayman products at
MyAssays.com.

If you have questions on the use of
our tools, or feel something is missing, please contact Technical
Services.

Antioxidant Analysis Tools

These resources are provided to serve the analysis needs of researchers using our Antioxidant Assay Kit.

If you have questions on the use of
our tools, or feel something is missing, please contact Technical
Services.

The Workbooks

Currently, Cayman Chemical uses two Microsoft Excel workbooks for analyzing antioxidant assays: AntiOxTriple and
AntiOxDouble. The first is formatted for assays performed in triplicate, and the second is formatted for assays
performed in duplicate.

Download

Your downloading these files implies acceptance of the license agreement listed below and present in
each workbook.

Workbook Sheets

Analysis

Raw absorbance values entered into the worksheet in the format defined by the LAYOUT section.

CONC

The concentration for each cell is calculated from the absorbance value, constants from the linear regression curve (see StdCurve section), and the dilution factor from the LAYOUT section.

STANDARDS

The Avg absorbance values are calculated for each concentration.

Samples

This table is compiled from the preceding sections. It includes

Conc: average of the replicate concentrations from the CONC section

Dilution: the dilution factor, for that sample, from the LAYOUT section

StdDev: the standard deviation of the replicate concentration values

%CV: the coefficient of variance of the replicate concentration values

SEM: the standard error of the mean for the replicate concentration values

STATS

y-int and slope are obtained from the log-regression fit of the calibration data.

FILENAME

The filename given to the Excel workbook

VERSION

The current version of the Excel workbook

StdCurve

This is the calibration curve of the concentration standards plotted versus their average absorbance values. The
data are fit to a linear regression line with the general equation: y = mx + b.

Instructions

The worksheets were designed to be as "hands-off" as possible. The following instructions assume you
have loaded your plate according to one of the plate layouts shown above, and have the data in a form that can be imported (or pasted) directly into a spreadsheet. To analyze your data:

Input your sample names into the appropriate cells

Input the sample dilutions into the appropriate cells in the format shown (e.g., 1:5)

Paste/type your data into the RAW DATA section (starting in cell B12) of the Analysis worksheet. Select the
"Values" option in the "Paste Special..." dialog box to maintain formatting

All concentration values, and relevant statistics, are automatically calculated. The Samples table is then filled in
with the data. All averages and statistics are calculated based on all replicates for each sample. It is the
responsibility of the individual researcher to decide if any data can be eliminated. Caution should be exercised,
however, when doing this! Cayman Chemical has examined statistical methods to test for the presence of outliers in
96-well assays. We find that individual replicates are rarely flagged as outliers, and cannot be eliminated from the
experiment.

Note The sole purpose of the data contained in this workbook is to demonstrate the functions of the
individual spreadsheets.

Copyright and Disclaimer

Redistribution and use in source and binary forms, with or without modification, are permitted
provided
that the
following conditions are met:

Redistributions of source code must retain the above copyright notice, this list of conditions
and
the following
disclaimer.

Redistributions in binary form must reproduce the above copyright notice, this list of
conditions
and the
following disclaimer in the documentation and/or other materials provided with the distribution.

THIS SOFTWARE IS PROVIDED BY CAYMAN CHEMICAL COMPANY, INC. "AS IS" AND ANY EXPRESS OR IMPLIED
WARRANTIES,
INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
PARTICULAR
PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL CAYMAN CHEMICAL BE LIABLE FOR ANY DIRECT, INDIRECT,
INCIDENTAL,
SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF
SUBSTITUTE
GOODS
OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY
THEORY
OF
LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
ARISING IN
ANY
WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF
THE POSSIBILITY OF SUCH DAMAGE.

The views and conclusions contained in the software and documentation are those of the authors and
should
not be interpreted as representing official policies, either expressed or implied, of Cayman
Chemical
Company, Inc.

Catalase Activity Analysis Tools

These resources are provided to serve the analysis needs of researchers using our Catalase Assay Kit.

If you have questions on the use of
our tools, or feel something is missing, please contact Technical
Services.

The Workbooks

Currently, Cayman Chemical uses two Microsoft Excel workbooks for analyzing Catalase assays: CatalaseTriple
and CatalaseDouble. The first is formatted for assays performed in triplicate, and the second is formatted
for assays performed in duplicate.

Download

Your downloading these files implies acceptance of the license agreement listed below and present in
each workbook.

CatalaseTriple~ 600 Kb XLS formatted for assays performed in triplicate

CatalaseDouble~ 600 Kb XLS formatted for assays performed in duplicate

Workbook Sheets

Analysis

Raw absorbance values entered into the worksheet in the format defined by the LAYOUT section.

CONC

The concentration for each cell is calculated from the absorbance value, constants from the linear regression curve (see StdCurve section), and the dilution factor from the LAYOUT section.

STANDARDS

The Avg corrected absorbance values are calculated for each concentration

Samples

This table is compiled from the preceding sections. It includes

Conc: average of the replicate concentrations from the CONC section

Dilution: the dilution factor, for that sample, from the LAYOUT section

StdDev: the standard deviation of the replicate concentration values

%CV: the coefficient of variance of the replicate concentration values

SEM: the standard error of the mean for the replicate concentration values

STATS

y-int and slope are obtained from the log-regression fit of the calibration data.

FILENAME

The filename given to the Excel workbook

VERSION

The current version of the Excel workbook

StdCurve

This is the calibration curve of the concentration standards plotted versus their average absorbance values. The
data are fit to a linear regression line with the general equation: y = mx + b.

Instructions

The worksheets were designed to be as "hands-off" as possible. The following
instructions assume you have loaded your plate according to one of the plate layouts shown above, and have
the data in a form that can be imported (or pasted) directly into a spreadsheet. To analyze your data:

Input your sample names into the appropriate cells

Input the sample dilutions into the appropriate cells in the format shown (e.g., 1:5)

Paste/type your data into the RAW DATA section (starting in cell B12) of the Analysis worksheet. Select the
"Values" option in the "Paste Special..." dialog box to maintain formatting

All concentration values, and relevant statistics, are automatically calculated. The Samples table is then
filled in with the data. All averages and statistics are calculated based on all replicates for each sample.
It is the responsibility of the individual researcher to decide if any data can be eliminated. Caution
should be exercised, however, when doing this! Cayman Chemical has examined statistical methods to test for
the presence of outliers in 96-well assays. We find that individual replicates are rarely flagged as
outliers, and cannot be eliminated from the experiment.

Note The sole purpose of the data contained in this workbook is to demonstrate the functions of the
individual spreadsheets.

Copyright and Disclaimer

Redistribution and use in source and binary forms, with or without modification, are permitted
provided
that the
following conditions are met:

Redistributions of source code must retain the above copyright notice, this list of conditions
and
the following
disclaimer.

Redistributions in binary form must reproduce the above copyright notice, this list of
conditions
and the
following disclaimer in the documentation and/or other materials provided with the distribution.

THIS SOFTWARE IS PROVIDED BY CAYMAN CHEMICAL COMPANY, INC. "AS IS" AND ANY EXPRESS OR IMPLIED
WARRANTIES,
INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
PARTICULAR
PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL CAYMAN CHEMICAL BE LIABLE FOR ANY DIRECT, INDIRECT,
INCIDENTAL,
SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF
SUBSTITUTE
GOODS
OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY
THEORY
OF
LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
ARISING IN
ANY
WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF
THE POSSIBILITY OF SUCH DAMAGE.

The views and conclusions contained in the software and documentation are those of the authors and
should
not be interpreted as representing official policies, either expressed or implied, of Cayman
Chemical
Company, Inc.

Creatinine Analysis Tools

These resources are provided to serve the analysis needs of researchers using our Creatinine Assay Kit.

If you have questions on the use of
our tools, or feel something is missing, please contact Technical
Services.

The Workbooks

Currently, Cayman Chemical uses two Microsoft Excel workbooks for analyzing Creatinine assays: CreatinineTriple and
CreatinineDouble. The first is formatted for assays performed in triplicate, and the second is formatted for assays
performed in duplicate.

Download

Your downloading these files implies acceptance of the license agreement listed below and present in
each workbook.

Workbook Sheets

Analysis

Data, from step 6 of the assay protocol, entered into the worksheet in the format defined by the LAYOUT section

FINAL (Fabs)

Data, from step 9 of the assay protocol, entered into the worksheet in the format defined by the LAYOUT section

CORRECTED

(Iabs - Fabs) calculated for each cell

ADJUSTED

Corrected absorbance values with the AvgBlnk subtracted

CONC

The concentration for each cell is calculated from the adjusted absorbance value, constants from the linear regression curve (see StdCurve section), and the dilution factor from the LAYOUT section

STANDARDS

The Avg corrected absorbance values are calculated for each concentration

Samples

This table is compiled from the preceding sections. It includes

Conc: average of the replicate concentrations from the CONC section

Dilution: the dilution factor, for that sample, from the LAYOUT section

StdDev: the standard deviation of the replicate concentration values

%CV: the coefficient of variance of the replicate concentration values

SEM: the standard error of the mean for the replicate concentration values

STATS

y-int and slope are obtained from the linear regression fit of the calibration data.

FILENAME

The filename given to the Excel workbook

VERSION

The current version of the Excel workbook

StdCurve

This is the calibration curve of the concentration standards plotted versus their absorbance values. The data are fit to a linear regression line with the general equation: y = mx + b.

Instructions

The worksheets were designed to be as "hands-off" as possible. The following instructions assume you have
loaded your plate according to one of the plate layouts shown above, and have the data in a form that can be
imported (or pasted) directly into a spreadsheet. To analyze your data:

Input your sample names into the appropriate cells

Input the sample dilutions into the appropriate cells in the format shown (e.g., 1:5)

Paste/type your data into the INITIAL and FINAL sections (starting in cells B12 and B22, respectively)
of the Analysis worksheet. Select the "Values" option in the "Paste Special..." dialog box to maintain
formatting.

All concentration values, and relevant statistics, are automatically calculated. The Samples table is then
filled in with the data. All averages and statistics are calculated based on all replicates for each sample.
It is the responsibility of the individual researcher to decide if any data can be eliminated. Caution
should be exercised, however, when doing this! Cayman Chemical has examined statistical methods to test for
the presence of outliers in creatinine assays. We find that individual replicates are rarely flagged as
outliers, and cannot be eliminated from the experiment.

Note The sole purpose of the data contained in this workbook is to demonstrate the functions of the
individual spreadsheets.

Copyright and Disclaimer

Redistribution and use in source and binary forms, with or without modification, are permitted
provided
that the
following conditions are met:

Redistributions of source code must retain the above copyright notice, this list of conditions
and
the following
disclaimer.

Redistributions in binary form must reproduce the above copyright notice, this list of
conditions
and the
following disclaimer in the documentation and/or other materials provided with the distribution.

THIS SOFTWARE IS PROVIDED BY CAYMAN CHEMICAL COMPANY, INC. "AS IS" AND ANY EXPRESS OR IMPLIED
WARRANTIES,
INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
PARTICULAR
PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL CAYMAN CHEMICAL BE LIABLE FOR ANY DIRECT, INDIRECT,
INCIDENTAL,
SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF
SUBSTITUTE
GOODS
OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY
THEORY
OF
LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
ARISING IN
ANY
WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF
THE POSSIBILITY OF SUCH DAMAGE.

The views and conclusions contained in the software and documentation are those of the authors and
should
not be interpreted as representing official policies, either expressed or implied, of Cayman
Chemical
Company, Inc.

ELISA (Competitive) Analysis Tools

These resources are provided to serve the analysis needs of researchers using our competitive ELISA kits.

If you have questions on the use of
our tools, or feel something is missing, please contact Technical
Services.

The Workbooks

Currently, Cayman Chemical uses two Excel workbooks to perform ELISA analysis. Both are available for your
download and use.

Download

Your downloading these files implies acceptance of the license agreement listed below and present in
each workbook.

Pop-up menu to choose the assay you are analyzing. Selecting an assay inserts the value of Std01 and its units into cells B70 and H69, respectively

Stats

'y-int', 'slope' and R2 are obtained from the log-regression fit of the calibration data. These values are automatically calculated and inserted into cells C79 through C81

Workbook Sheets

Analysis

Field

Explanation

LAYOUT

Contains sample names and associated dilution factors

RAW DATA

Data entered into the worksheet in the format defined by the LAYOUT section

SUBTRACTED

The AvgRawBlnk is subtracted from each cell

B/B0

The value of each cell is computed by subtracting the AvgNSB and AvgBlank from the SUBTRACTED value, and then dividing by the AvgBo. The result of each cell is evaluated to determine if it is within the 20-80% B/B0 linear range of the calibration curve, and highlighted accordingly

LOGIT

The logit for each cell is computed using the forumula: ln(B/B0/((1 - B/B0)). This technique "linearizes" the values to facilitate regression analysis

CONC

The concentration for each cell is calculated based on the LOGIT value, constants from the log-regression curve (see StdCurve section), and the dilution factor from the LAYOUT section

STANDARDS

The AvgB/B0Logit and Recalc values are calculated for each concentration value. Only the value of Std01 must be entered; subsequent values are calculated automatically. Recalc are the standard concentration values calculated from the regression constants

Samples

This table is compiled from the preceding sections. It includes

Conc: average of the replicate concentrations from the CONCN section. Data outside the 20-80% B/B0 range are highlighted

%B/B0: average of the replicate values from the B/B0 section expressed as a percentage

Dilution: the dilution factor, for that sample, from the LAYOUT section

StdDev: the standard deviation of the replicate concentration values

%CV: the coefficient of variance of the replicate concentration values

SEM: the standard error of the mean for the replicate concentration values

STATS

y-int and slope are obtained from the log-regression fit of the calibration data. These values must be manuallytransferred from the StdCurve worksheet

CURVE

The IC20, IC50, and IC80 values are calculated. The IC20 and IC80 are the upper and lower detection limits of the standard curve, respectively. The IC50 is a quality control parameter (given as 50% B/B0 in the kit booklet)

% Error

The absolute percent error of the control concentrations from their predicted value

StdCurve

This is the calibration curve of the concentration standards plotted versus the logit(B/B0)
values. The data are fit to a logarithmic regression line with the general equation: y = m ln(x) + b. The
regression equation is displayed along with the correlation coefficient.

Instructions

The worksheets were designed to be as "hands-off" as possible. The following instructions assume you
have loaded
your plate according to one of the plate layouts shown above, and have the data in a form that can be imported (or
pasted) directly into a spreadsheet. To analyze your data:

Choose the assay you wish to analyze with the pop-up menu

Input your sample names into the appropriate cells

Input the sample dilutions into the appropriate cells in the format shown (e.g., 1:5)

Paste/type your data into the RAW DATA section (starting in cell B12) of the Analysis worksheet. Select the
"Values" option in the "Paste Special..." dialog box to maintain formatting

All concentration values, and relevant statistics, are automatically calculated. The Samples table is then
filled in with the data. All averages and statistics are calculated based on all replicates for each sample. It is
the responsibility of the individual researcher to decide if any data can be eliminated. Caution should be
exercised, however, when doing this! Cayman Chemical has examined statistical methods to test for the presence
of outliers in ELISA assays. We find that individual replicates are rarely flagged as outliers, and cannot be
eliminated from the experiment.

Note The sole purpose of the data contained in this workbook is to demonstrate the functions of the
individual spreadsheets. It should not be used to determine any experimental parameters (e.g., standard dilutions, etc.).

Copyright and Disclaimer

Redistribution and use in source and binary forms, with or without modification, are permitted
provided
that the
following conditions are met:

Redistributions of source code must retain the above copyright notice, this list of conditions
and
the following
disclaimer.

Redistributions in binary form must reproduce the above copyright notice, this list of
conditions
and the
following disclaimer in the documentation and/or other materials provided with the distribution.

THIS SOFTWARE IS PROVIDED BY CAYMAN CHEMICAL COMPANY, INC. "AS IS" AND ANY EXPRESS OR IMPLIED
WARRANTIES,
INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
PARTICULAR
PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL CAYMAN CHEMICAL BE LIABLE FOR ANY DIRECT, INDIRECT,
INCIDENTAL,
SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF
SUBSTITUTE
GOODS
OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY
THEORY
OF
LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
ARISING IN
ANY
WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF
THE POSSIBILITY OF SUCH DAMAGE.

The views and conclusions contained in the software and documentation are those of the authors and
should
not be interpreted as representing official policies, either expressed or implied, of Cayman
Chemical
Company, Inc.

ELISA (Immunometric/Sandwich) Analysis Tools

These resources are provided to serve the analysis needs of researchers using our immunometric/sandwich ELISA kits.

If you have questions on the use of
our tools, or feel something is missing, please contact Technical
Services.

The Workbooks

Currently, Cayman Chemical uses two Excel workbooks to perform immunometric analysis. Both are available for
your download and use.

Download

Your downloading these files implies acceptance of the license agreement listed below and present in
each workbook.

Workbook Sheets

Analysis

Data entered into the worksheet in the format defined by the LAYOUT section

SUBTRACTED

The AvgRawBlnk is subtracted from each cell

CONC

The concentration for each cell is calculated based on the absorbance value, constants from the linear regression curve (see StdCurve section), and the dilution factor from the LAYOUT section

STANDARDS

The Avg and Recalc values are calculated for each concentration value. Recalc are the standard concentration values calculated from the regression constants

Samples

This table is compiled from the preceding sections. It includes

Conc: average of the replicate concentrations from the CONCN section.

Dilution: the dilution factor, for that sample, from the LAYOUT section

StdDev: the standard deviation of the replicate concentration values

%CV: the coefficient of variance of the replicate concentration values

SEM: the standard error of the mean for the replicate concentration values

STATS

y-int and slope are obtained from the linear regression fit of the calibration data.

FILENAME

The filename given to the Excel workbook.

StdCurve

This is the calibration curve of the concentration standards plotted versus their absorbance values. The
data are fit to a linear regression line with the general equation: y = m x + b.

Instructions

The worksheets were designed to be as "hands-off" as possible. The following instructions assume you
have
loaded your plate according to one of the plate layouts shown above, and have the data in a form that can be
imported (or pasted) directly into a spreadsheet. To analyze your data:

Select your assay from the pull-down, scrollable menu.

Input your sample names into the appropriate cells

Input the sample dilutions into the appropriate cells in the format shown (e.g., 1:5)

Paste/type your data into the RAW DATA section (starting in cell B14) of the Analysis worksheet.
Select the "Values" option in the "Paste Special..." dialog box to maintain formatting

All concentration values, and relevant statistics, are automatically calculated. The Samples table is
then filled in with the data. All averages and statistics are calculated based on all replicates for each
sample. It is the responsibility of the individual researcher to decide if any data can be eliminated. Caution
should be exercised, however, when doing this! Cayman Chemical has examined statistical methods to test
for the presence of outliers in immunometric assays. We find that individual replicates are rarely flagged
as outliers, and cannot be eliminated from the experiment.

Note The sole purpose of the data contained in this workbook is to demonstrate the functions of the
individual spreadsheets.

Copyright and Disclaimer

Redistribution and use in source and binary forms, with or without modification, are permitted
provided
that the
following conditions are met:

Redistributions of source code must retain the above copyright notice, this list of conditions
and
the following
disclaimer.

Redistributions in binary form must reproduce the above copyright notice, this list of
conditions
and the
following disclaimer in the documentation and/or other materials provided with the distribution.

THIS SOFTWARE IS PROVIDED BY CAYMAN CHEMICAL COMPANY, INC. "AS IS" AND ANY EXPRESS OR IMPLIED
WARRANTIES,
INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
PARTICULAR
PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL CAYMAN CHEMICAL BE LIABLE FOR ANY DIRECT, INDIRECT,
INCIDENTAL,
SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF
SUBSTITUTE
GOODS
OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY
THEORY
OF
LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
ARISING IN
ANY
WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF
THE POSSIBILITY OF SUCH DAMAGE.

The views and conclusions contained in the software and documentation are those of the authors and
should
not be interpreted as representing official policies, either expressed or implied, of Cayman
Chemical
Company, Inc.

FPIA 384 Wells

These resources are provided to serve the analysis needs of researchers using our 384-well fluorescence polarization immunoassay kits.

If you have questions on the use of
our tools, or feel something is missing, please contact Technical
Services.

The Workbooks

Currently, Cayman Chemical uses two Excel workbooks to perform 384 well FPIA analysis. Both are available
for your download and use.

Download

Your downloading these files implies acceptance of the license agreement listed below and present in
each workbook.

Fluorescence of the sample, prior to addition of the fluorescent reagent, with the emission polarizer set parallel to the excitation polarizer.

BKG-PERP

Fluorescence of the sample, prior to addition of the fluorescent reagent, with the emission polarizer set perpendicular to the excitation polarizer.

PARALLEL

Fluorescence of the sample, after addition of the fluorescent reagent, with the emission polarizer set parallel to the excitation polarizer.

PERPENDICULAR

Fluorescence of the sample, after addition of the fluorescent reagent, with the emission polarizer set perpendicular to the excitation polarizer.

mP

Calculated using the following equation:

mP = 1000 x

(Iparl - Iperp)

(Iparl + Iperp)

LOGIT

The logit for each cell is computed using the formula: ln[(mP/mPmax)/((1 - (mP/mPmax))]. This technique 'linearizes' the values to facilitate regression analysis.

CONC

The concentration for each cell is calculated based on the LOGIT value, constants from the log-regression curve (see StdCurve section), and the dilution factor from the LAYOUT section.

STANDARDS

The 'Avg', 'mP/mPmax', 'Logit', and 'Recalc' values are calculated for each concentration value. Only the value of 'Std01' must be entered; subsequent values are calculated automaticallly. 'Recalc' are the standard concentration values calculated from the regression constraints.

Samples

This table is compiled from the preceding sections. It includes

Conc: average of the replicate concentrations from the CONC section. Data outside the 20-80% mP/mP(max) range are highlighted.

mP/mPmax: average of the replicate values from the mP/mPmax section expressed as a percentage.

Dilution: the dilution factor, for the sample, from the LAYOUT section.

StdDev: the standard deviation of the replicate concentration values.

%CV: the coefficient of variance of the replicate concentration values.

SEM: the standard error of the mean for the replicate concentration values.

STATS

'y-int' and 'slope' are obtained from the log-regression fit of the calibration data.

CURVE

The IC20, IC50, and IC80 values are calculated. The IC20 & IC80 are the upper and lower detection limits of the standard curve, respectively. The IC50 is a quality control paramater (given as 50% mP/mPmax).

FILENAME

The filename given to the Excel workbook.

VERSION

The version of the Excel workbook.

StdCurve

This is the calibration curve of the concentration standards plotted versus the logit(mP/mPmax)
values. The data are fit to a logarithmic regression line with the general equation:
y = mln(x) + b.

Instructions

The worksheets were designed to be as "hands-off" as possible. The following instructions assume you
have
loaded your plate according to one of the plate layouts shown on the Workbooks sheet, and have the data in a
form that can be imported (or pasted) directly into a spreadsheet. To analyze your data:

Select the assay you are running from the pull down menu.

Input your sample names into the appropriate cells.

Input the sample dilutions into the appropriate cells in the format shown (e.g., 1:5).

Paste/type your data into the RAW DATA section (starting in cell B22) of the Analysis worksheet.

Select the "Values" option in the "Paste Special…" dialog box to maintain formatting. All concentration
values, and relevant statistics, are automatically calculated. The Samples table is then filled in with the
data. All averages and statistics are calculated based on all replicates for each sample. It is the
responsibility of the individual researcher to decide if any data can be eliminated. Caution should be
exercised, however, when doing this! Cayman Chemical has examined statistical methods to test for the
presence of outliers in EIA assays. We find that individual replicates are rarely flagged as outliers, and
cannot be eliminated from the experiment.

NOTE: The sole purpose of the data contained in this workbook is to demonstrate the functions of the
individual spreadsheets.

Copyright and Disclaimer

Redistribution and use in source and binary forms, with or without modification, are permitted
provided
that the
following conditions are met:

Redistributions of source code must retain the above copyright notice, this list of conditions
and
the following
disclaimer.

Redistributions in binary form must reproduce the above copyright notice, this list of
conditions
and the
following disclaimer in the documentation and/or other materials provided with the distribution.

THIS SOFTWARE IS PROVIDED BY CAYMAN CHEMICAL COMPANY, INC. "AS IS" AND ANY EXPRESS OR IMPLIED
WARRANTIES,
INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
PARTICULAR
PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL CAYMAN CHEMICAL BE LIABLE FOR ANY DIRECT, INDIRECT,
INCIDENTAL,
SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF
SUBSTITUTE
GOODS
OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY
THEORY
OF
LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
ARISING IN
ANY
WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF
THE POSSIBILITY OF SUCH DAMAGE.

The views and conclusions contained in the software and documentation are those of the authors and
should
not be interpreted as representing official policies, either expressed or implied, of Cayman
Chemical
Company, Inc.

FPIA 96 Wells

These resources are provided to serve the analysis needs of researchers using our 96-well fluorescence polarization immunoassay kits.

If you have questions on the use of
our tools, or feel something is missing, please contact Technical
Services.

The Workbooks

Currently, Cayman Chemical uses two Excel workbooks to perform 96 well FPIA analysis. Both are available for
your download and use.

Download

Your downloading these files implies acceptance of the license agreement listed below and present in
each workbook.

Fluorescence of the sample, prior to addition of the fluorescent reagent, with the emission polarizer set parallel to the excitation polarizer.

BKG-PERP

Fluorescence of the sample, prior to addition of the fluorescent reagent, with the emission polarizer set perpendicular to the excitation polarizer.

PARALLEL

Fluorescence of the sample, after addition of the fluorescent reagent, with the emission polarizer set parallel to the excitation polarizer.

PERPENDICULAR

Fluorescence of the sample, after addition of the fluorescent reagent, with the emission polarizer set perpendicular to the excitation polarizer.

mP

Calculated using the following equation:

mP = 1000 x

(Iparl - Iperp)

(Iparl + Iperp)

LOGIT

The logit for each cell is computed using the formula: ln[(mP/mPmax)/((1 - (mP/mPmax))]. This technique 'linearizes' the values to facilitate regression analysis.

CONC

The concentration for each cell is calculated based on the LOGIT value, constants from the log-regression curve (see StdCurve section), and the dilution factor from the LAYOUT section.

STANDARDS

The 'Avg', 'mP/mPmax', 'Logit', and 'Recalc' values are calculated for each concentration value. Only the value of 'Std01' must be entered; subsequent values are calculated automaticallly. 'Recalc' are the standard concentration values calculated from the regression constraints.

Samples

This table is compiled from the preceding sections. It includes

Conc: average of the replicate concentrations from the CONC section. Data outside the 20-80% mP/mP(max) range are highlighted.

mP/mPmax: average of the replicate values from the mP/mPmax section expressed as a percentage.

Dilution: the dilution factor, for the sample, from the LAYOUT section.

StdDev: the standard deviation of the replicate concentration values.

%CV: the coefficient of variance of the replicate concentration values.

SEM: the standard error of the mean for the replicate concentration values.

STATS

'y-int' and 'slope' are obtained from the log-regression fit of the calibration data.

CURVE

The IC20, IC50, and IC80 values are calculated. The IC20 & IC80 are the upper and lower detection limits of the standard curve, respectively. The IC50 is a quality control paramater (given as 50% mP/mPmax).

FILENAME

The filename given to the Excel workbook.

VERSION

The version of the Excel workbook.

StdCurve

This is the calibration curve of the concentration standards plotted versus the logit(mP/mPmax)
values. The data are fit to a logarithmic regression line with the general equation: y = mln(x) + b.

Instructions

The worksheets were designed to be as "hands-off" as possible. The following instructions assume you
have loaded your plate according to one of the plate layouts shown on the Workbooks sheet, and have the data in a
form that can be imported (or pasted) directly into a spreadsheet. To analyze your data:

Select the assay you are running from the pull down menu.

Input your sample names into the appropriate cells.

Input the sample dilutions into the appropriate cells in the format shown (e.g., 1:5).

Paste/type your data into the RAW DATA section (starting in cell B14) of the Analysis worksheet. Select
the "Values" option in the "Paste Special…" dialog box to maintain formatting.

All concentration values, and relevant statistics, are automatically calculated. The Samples table is then
filled in with the data. All averages and statistics are calculated based on all replicates for each sample.
It is the responsibility of the individual researcher to decide if any data can be eliminated. Caution
should be exercised, however, when doing this! Cayman Chemical has examined statistical methods to test for
the presence of outliers in EIA assays. We find that individual replicates are rarely flagged as outliers,
and cannot be eliminated from the experiment.

NOTE: The sole purpose of the data contained in this workbook is to demonstrate the functions of the
individual spreadsheets.

Copyright and Disclaimer

Redistribution and use in source and binary forms, with or without modification, are permitted
provided
that the
following conditions are met:

Redistributions of source code must retain the above copyright notice, this list of conditions
and
the following
disclaimer.

Redistributions in binary form must reproduce the above copyright notice, this list of
conditions
and the
following disclaimer in the documentation and/or other materials provided with the distribution.

THIS SOFTWARE IS PROVIDED BY CAYMAN CHEMICAL COMPANY, INC. "AS IS" AND ANY EXPRESS OR IMPLIED
WARRANTIES,
INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
PARTICULAR
PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL CAYMAN CHEMICAL BE LIABLE FOR ANY DIRECT, INDIRECT,
INCIDENTAL,
SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF
SUBSTITUTE
GOODS
OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY
THEORY
OF
LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
ARISING IN
ANY
WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF
THE POSSIBILITY OF SUCH DAMAGE.

The views and conclusions contained in the software and documentation are those of the authors and
should
not be interpreted as representing official policies, either expressed or implied, of Cayman
Chemical
Company, Inc.

Workbook Sheets

Analysis

Data entered into the worksheet in the format defined by the LAYOUT section

SUBTRACTED

The AvgRawBlnk is subtracted from each cell

CONC

The concentration for each cell is calculated based on the absorbance value, constants from the linear regression curve (see StdCurve section), and the dilution factor from the LAYOUT section

STANDARDS

The Avg and Recalc values are calculated for each concentration value. Recalc are the standard concentration values calculated from the regression constants

Samples

This table is compiled from the preceding sections. It includes

Conc: average of the replicate concentrations from the CONCN section.

Dilution: the dilution factor, for that sample, from the LAYOUT section

StdDev: the standard deviation of the replicate concentration values

%CV: the coefficient of variance of the replicate concentration values

SEM: the standard error of the mean for the replicate concentration values

STATS

y-int and slope are obtained from the linear regression fit of the calibration data.

FILENAME

The filename given to the Excel workbook.

StdCurve

This is the calibration curve of the concentration standards plotted versus their absorbance values. The
data are fit to a linear regression line with the general equation: y = m x + b.

Instructions

The worksheets were designed to be as "hands-off" as possible. The following instructions assume you
have loaded your plate according to one of the plate layouts shown above, and have the data in a form that can be
imported (or pasted) directly into a spreadsheet. To analyze your data:

Select your assay from the pull-down, scrollable menu.

Input your sample names into the appropriate cells

Input the sample dilutions into the appropriate cells in the format shown (e.g., 1:5)

Paste/type your data into the RAW DATA section (starting in cell B14) of the Analysis worksheet.
Select the "Values" option in the "Paste Special..." dialog box to maintain formatting

All concentration values, and relevant statistics, are automatically calculated. The Samples table is
then filled in with the data. All averages and statistics are calculated based on all replicates for each
sample. It is the responsibility of the individual researcher to decide if any data can be eliminated. Caution
should be exercised, however, when doing this! Cayman Chemical has examined statistical methods to test
for the presence of outliers in GSH Activity assays. We find that individual replicates are rarely flagged
as outliers, and cannot be eliminated from the experiment.

Note The sole purpose of the data contained in this workbook is to demonstrate the functions of the
individual spreadsheets.

Copyright and Disclaimer

Redistribution and use in source and binary forms, with or without modification, are permitted
provided
that the
following conditions are met:

Redistributions of source code must retain the above copyright notice, this list of conditions
and
the following
disclaimer.

Redistributions in binary form must reproduce the above copyright notice, this list of
conditions
and the
following disclaimer in the documentation and/or other materials provided with the distribution.

THIS SOFTWARE IS PROVIDED BY CAYMAN CHEMICAL COMPANY, INC. "AS IS" AND ANY EXPRESS OR IMPLIED
WARRANTIES,
INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
PARTICULAR
PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL CAYMAN CHEMICAL BE LIABLE FOR ANY DIRECT, INDIRECT,
INCIDENTAL,
SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF
SUBSTITUTE
GOODS
OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY
THEORY
OF
LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
ARISING IN
ANY
WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF
THE POSSIBILITY OF SUCH DAMAGE.

The views and conclusions contained in the software and documentation are those of the authors and
should
not be interpreted as representing official policies, either expressed or implied, of Cayman
Chemical
Company, Inc.

These resources are provided to serve the analysis needs of researchers using our glutathione peroxidase, glutathione reductase, and glutathione S-transferase assays.

If you have questions on the use of
our tools, or feel something is missing, please contact Technical
Services.

The Workbooks

Currently, Cayman Chemical uses two Microsoft Excel workbooks for analyzing GSHases assays: GSHasesTriple
and GSHasesDouble. The first is formatted for assays performed in triplicate, and the second is formatted
for assays performed in duplicate.

Download

Your downloading these files implies acceptance of the license agreement listed below and present in
each workbook.

GSHasesTriple~ 600 Kb XLS
formatted for assays performed in triplicate

For each control and sample, the absorbance value at each time point is averaged. The linear regression statistics (i.e., slope and y-intercept) and reaction rate are then calculated

RESULTS

This table is compiled from the preceding sections. It includes the sample name, assay dilution and activity

FILENAME

The filename given to the Excel workbook

VERSION

The version of the workbook

StdCurve

These are the kinetic curves for each sample. The reaction time is plotted versus the
absorbance of the sample at 340 nm.

Instructions

The worksheets were designed to be as "hands-off" as possible. The following instructions assume you
have loaded your plate according to one of the plate layouts shown above, and have the data in a form that can be
imported (or pasted) directly into a spreadsheet. To analyze your data:

Select, from the pull down menu, which enzyme assay you are running

Input your sample names into the appropriate cells

Input the sample dilutions into the appropriate cells in the format shown (e.g., 1:5)

All activity values, and relevant statistics, are automatically calculated. The RESULTS table is then filled
in with the data. All averages and statistics are calculated based on all replicates for each sample. It is
the responsibility of the individual researcher to decide if any data can be eliminated. Caution should be
exercised, however, when doing this! Cayman Chemical has examined statistical methods to test for the
presence of outliers in 96-well based assays. We find that individual replicates are rarely flagged as
outliers, and cannot be eliminated from the experiment.

Note The sole purpose of the data contained in this workbook is to demonstrate the functions of the
individual spreadsheets.

Copyright and Disclaimer

Redistribution and use in source and binary forms, with or without modification, are permitted
provided
that the
following conditions are met:

Redistributions of source code must retain the above copyright notice, this list of conditions
and
the following
disclaimer.

Redistributions in binary form must reproduce the above copyright notice, this list of
conditions
and the
following disclaimer in the documentation and/or other materials provided with the distribution.

THIS SOFTWARE IS PROVIDED BY CAYMAN CHEMICAL COMPANY, INC. "AS IS" AND ANY EXPRESS OR IMPLIED
WARRANTIES,
INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
PARTICULAR
PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL CAYMAN CHEMICAL BE LIABLE FOR ANY DIRECT, INDIRECT,
INCIDENTAL,
SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF
SUBSTITUTE
GOODS
OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY
THEORY
OF
LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
ARISING IN
ANY
WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF
THE POSSIBILITY OF SUCH DAMAGE.

The views and conclusions contained in the software and documentation are those of the authors and
should
not be interpreted as representing official policies, either expressed or implied, of Cayman
Chemical
Company, Inc.

Lipoxygenase Inhibitor Screening (LIS) Analysis Tools

These resources are provided to serve the analysis needs of researchers using our Lipoxygenase Inhibitor Screening Assay Kit.

If you have questions on the use of
our tools, or feel something is missing, please contact Technical
Services.

The Workbooks

Currently, Cayman Chemical uses two Microsoft Excel workbooks for analyzing hydrogen peroxide assays:
LISTriple and LISDouble. The first is formatted for assays performed in triplicate, and the second is
formatted for assays performed in duplicate.

Download

Your downloading these files implies acceptance of the license agreement listed below and present in
each workbook.

Workbook Sheets

Analysis

Absorbance value (between 490-500 nm) of each well after two 5 minute incubations

ACTIVITY

For each absorbance value, the activity of the well is calculated using the method on page 15 of the kit booklet

RESULTS

This table is compiled from the preceding sections. It includes the sample name, assay dilution and percent inhibition of the compound. The inhibition is calculated using the method on page 16 in the kit booklet

Copyright and Disclaimer

Redistribution and use in source and binary forms, with or without modification, are permitted
provided
that the
following conditions are met:

Redistributions of source code must retain the above copyright notice, this list of conditions
and
the following
disclaimer.

Redistributions in binary form must reproduce the above copyright notice, this list of
conditions
and the
following disclaimer in the documentation and/or other materials provided with the distribution.

THIS SOFTWARE IS PROVIDED BY CAYMAN CHEMICAL COMPANY, INC. "AS IS" AND ANY EXPRESS OR IMPLIED
WARRANTIES,
INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
PARTICULAR
PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL CAYMAN CHEMICAL BE LIABLE FOR ANY DIRECT, INDIRECT,
INCIDENTAL,
SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF
SUBSTITUTE
GOODS
OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY
THEORY
OF
LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
ARISING IN
ANY
WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF
THE POSSIBILITY OF SUCH DAMAGE.

The views and conclusions contained in the software and documentation are those of the authors and
should
not be interpreted as representing official policies, either expressed or implied, of Cayman
Chemical
Company, Inc.

Protein Analysis Tools

These resources are provided to serve the analysis needs of researchers using our Protein Determination Kit.

If you have questions on the use of
our tools, or feel something is missing, please contact Technical
Services.

The Workbooks

Currently, Cayman Chemical uses two Excel workbooks for analyzing protein assays. Both are available for
your download and use.

Download

Your downloading these files implies acceptance of the license agreement listed below and present in
each workbook.

ProteinTriple~ 600 Kb XLS formatted for assays performed in triplicate

Definition of Key Terms

Standards A through H made by serially diluting StdH six times. StdA is the zero-point.

Smpl# (1:#)

Samples and their associated dilution factors [Dilutions must be entered in the format shown]

Workbook Sheets

Analysis

Field

Explanation

LAYOUT

Contains sample names and associated dilution factors

RAW DATA

Data entered into the worksheet in the format defined by the LAYOUT section

CONC

The concentration for each cell is calculated based on the absorbance value, constants from the polynomial fit curve (see StdCurve section), and the dilution factor from the LAYOUT section

STANDARDS

The Avg and Avg2 values are calculated for each concentration value.

Samples

This table is compiled from the preceding sections. It includes

Conc: average of the replicate concentrations from the CONC section.

Dilution: the dilution factor, for that sample, from the LAYOUT section

StdDev: the standard deviation of the replicate concentration values

%CV: the coefficient of variance of the replicate concentration values

SEM: the standard error of the mean for the replicate concentration values

STATS

A, B, and C are obtained from the polynomial y = Ax2 +Bx + C fit of the calibration data.

FILENAME

The filename given to the Excel workbook.

StdCurve

This is the calibration curve of the concentration standards plotted versus their absorbance values. The
data are fit to a polynomial line with the general equation y = Ax2 + Bx + C.

Instructions

The worksheets were designed to be as "hands-off" as possible. The following instructions assume you
have loaded your plate according to one of the plate layouts shown above, and have the data in a form that can be
imported (or pasted) directly into a spreadsheet. To analyze your data:

Input your sample names into the appropriate cells.

Input the sample dilutions into the appropriate cells in the format shown (e.g., 1:5)

Paste/type your data into the RAW DATA section (starting in cell B12) of the Analysis worksheet.
Select the Values option in the Paste Special... dialog box to maintain formatting.

All concentration values, and relevant statistics, are automatically calculated. The Samples table is
then filled in with the data. All averages and statistics are calculated based on all replicates for each
sample. It is the responsibility of the individual researcher to decide if any data can be eliminated. Caution
should be exercised, however, when doing this! Cayman Chemical has examined statistical methods to test
for the presence of outliers in protein assays. We find that individual replicates are rarely flagged as
outliers, and cannot be eliminated from the experiment.

Note The sole purpose of the data contained in this workbook is to demonstrate the functions of the
individual spreadsheets.

Copyright and Disclaimer

Redistribution and use in source and binary forms, with or without modification, are permitted
provided
that the
following conditions are met:

Redistributions of source code must retain the above copyright notice, this list of conditions
and
the following
disclaimer.

Redistributions in binary form must reproduce the above copyright notice, this list of
conditions
and the
following disclaimer in the documentation and/or other materials provided with the distribution.

THIS SOFTWARE IS PROVIDED BY CAYMAN CHEMICAL COMPANY, INC. "AS IS" AND ANY EXPRESS OR IMPLIED
WARRANTIES,
INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
PARTICULAR
PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL CAYMAN CHEMICAL BE LIABLE FOR ANY DIRECT, INDIRECT,
INCIDENTAL,
SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF
SUBSTITUTE
GOODS
OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY
THEORY
OF
LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
ARISING IN
ANY
WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF
THE POSSIBILITY OF SUCH DAMAGE.

The views and conclusions contained in the software and documentation are those of the authors and
should
not be interpreted as representing official policies, either expressed or implied, of Cayman
Chemical
Company, Inc.

SOD Activity Analysis Tools

These resources are provided to serve the analysis needs of researchers using our Superoxide Dismutase Assay Kit.

If you have questions on the use of
our tools, or feel something is missing, please contact Technical
Services.

The Workbooks

Currently, Cayman Chemical uses two Excel workbooks to perform SOD Activity analysis. Both are available for
your download and use.

Download

Your downloading these files implies acceptance of the license agreement listed below and present in
each workbook.

Workbook Sheets

Analysis

Data entered into the worksheet in the format defined by the LAYOUT section. Data greater than the highest standard (ULOQ) or less than the lowest standard (LLOQ) are highlighted.

LINRZD RATE

For each cell, Blank is divided by the cell absorbance to give the linearized rate

ACTIVITY

The activity for each cell is calculated using the linearized rate, constants from the linear-regression curve, and the dilution factor from the LAYOUT section.

STANDARDS

The average linearized rates (Avg LR) are calculated for each activity value

Samples

This table is compiled from the preceding sections. It includes

U/mL: average of the replicate activities from the ACTIVITY section.

Dilution: the dilution factor, for that sample, from the LAYOUT section

StdDev: the standard deviation of the replicate concentration values

SD: the standard deviation of the replicate activity values.

%CV: the coefficient of variance of the replicate activity values.

STATS

slope, y-int, and R2 are obtained from the linear-regression fit of the standard curve data.

StdCurve

This is the calibration curve of the standard activities plotted versus their linearized rates. The data are
fit to a linear-regression line with the general equation: y = m x + b.

Instructions

The worksheets were designed to be as "hands-off" as possible. The following instructions assume you
have
loaded your plate according to one of the plate layouts shown above, and have the data in a form that can be
imported (or pasted) directly into a spreadsheet. To analyze your data:

Input your sample names into the appropriate cells

Input the sample dilutions into the appropriate cells in the format shown (e.g., 1:5)

Paste/type your data into the RAW DATA section (starting in cell B12) of the EndPoint worksheet.
Select the "Values" option in the "Paste Special…" dialog box to maintain formatting

All activity values, and relevant statistics, are automatically calculated. The Samples table is then
filled in with the data. All averages and statistics are calculated based on all replicates for each sample.
It is the responsibility of the individual researcher to decide if any data can be eliminated. Caution
should be exercised, however, when doing this! Cayman Chemical has examined statistical methods to test
for the presence of outliers in 96-well based assays. We find that individual replicates are rarely flagged
as outliers, and cannot be eliminated from the experiment.

Note The sole purpose of the data contained in this workbook is to demonstrate the functions of the
individual spreadsheets.

Copyright and Disclaimer

Redistribution and use in source and binary forms, with or without modification, are permitted
provided
that the
following conditions are met:

Redistributions of source code must retain the above copyright notice, this list of conditions
and
the following
disclaimer.

Redistributions in binary form must reproduce the above copyright notice, this list of
conditions
and the
following disclaimer in the documentation and/or other materials provided with the distribution.

THIS SOFTWARE IS PROVIDED BY CAYMAN CHEMICAL COMPANY, INC. "AS IS" AND ANY EXPRESS OR IMPLIED
WARRANTIES,
INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
PARTICULAR
PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL CAYMAN CHEMICAL BE LIABLE FOR ANY DIRECT, INDIRECT,
INCIDENTAL,
SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF
SUBSTITUTE
GOODS
OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY
THEORY
OF
LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
ARISING IN
ANY
WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF
THE POSSIBILITY OF SUCH DAMAGE.

The views and conclusions contained in the software and documentation are those of the authors and
should
not be interpreted as representing official policies, either expressed or implied, of Cayman
Chemical
Company, Inc.