Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Details on sampling- Concentrations: All; sampled at test initiation, 24 hours, and at test termination.- Sampling method: not described- Sample storage conditions before analysis: Samples were prepared for analysis on the day the samples were collected, and stored within autosampler vials. Samples were analyzed on the day collected, or the following day.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Method: Stirring of primary stock (4000 mg a.i./L) by inversion and and stirring. Dilution of primary stock with dilution water to obtain lower concentrations. - Controls: Blank test medium- Evidence of undissolved material: All solutions appeared clear and colorless.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM- Common name: Cladoceran; Water flea- Source: Cultures maintained by the test laboratory with known history- Age at study initiation: < 24 hours, from parental daphnids of more than two weeks old, which were observed to have no neonates present less than 24 hours prior to test initiation- Culturing: Adult daphnids were cultured in water from the same source and at approximately the same temperature as used during the test. Adult daphnids in the cultures were held for at least 14 days prior to collection of the juveniles for testing. The adults showed no signs of disease or stress during the holding period. During the 14-day period preceding the test, water temperatures ranged from 19.1 to 20.2°C. The pH of the water ranged from 8.2 to 8.5, and dissolved oxygen ranged from 8.0 to 8.6 mg/L. Daphnids in the cultures were fed a mixture of yeast, Cerophyl®, and trout chow, as well as a suspension of the freshwater green alga, Selenastrum capricornutum. The adults were fed prior to test initiation, but neonates were not fed during the test.FEEDING DURING TEST- Food type: Not fed during test

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

the validity criteria of OECD TG 202 were fulfilled. Control immobilization was < 10% at the end of the test. The dissolved oxygen concentration was = 3 mg/L in the control and test vessels at the end of the test.

The test was conducted according to internationally accepted test guidelines and was GLP compliant. It is reliable without restriction and suitable for Risk Assessment, Classification and Labeling, and PBT Analysis.

Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

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