The table lists the proteins and GenBank™ accession numbers identified by mass spectrometry in the large scale experiment by enrichment with anti-pSer/Thr antibody. Data on phosphorylation and corresponding references are indicated. The band numbers correspond to the bands excised from the silver-stained gel shown in Fig. 3.

Band excised

Protein name

Phosphorylation data

Accession no.

References

Band 1

Filamin-1

Phosphorylated on serine 2152 (PKA site)

NP_001447

17, 18, 20, 21

Band 2

Myosin heavy chain A

Phosphorylated on threonine residues 1823, 1833, and 2029 in Dictyostelium

NP_002464

23, 24

Band 3

Drebrin 1

Phosphorylated in vivo

NP_004386

27, 30

Band 4

α-Actinin 4

Phosphorylated in N-terminal region by protein kinase N. Phosphorylated in vitro by FAK on residue 12

NP_004915

33, 35–37

Band 5

Frigg (KIAA0668)

Phosphorylated on serine 12, 54, and 116 (additional sites present)

BAA31643

This study

Band 6

PABP2

Phosphorylation of PABP, a protein related to PABP2, specifies the type of binding to poly(A) RNA and to other proteins