Summary and Analysis

Oxidative Phosphorylation and Electron Transport

Problems

Problems

ATP synthesis is not an energetically favorable reaction: energy is needed
in order for it to occur. This energy is derived from the oxidation of NADH
and FADH2 by the four protein complexes of the electron transport chain
(ETC). The ten NADH that enter the electron transport originate from each of
the earlier processes of respiration: two from
glycolysis, two from the
transformation of pyruvate into acetyl-CoA,
and six from the citric acid cycle. The
two FADH2 originate in the citric acid cycle.

Figure %: The Electron Transport Chain.

The events of the electron transport chain involve NADH and FADH, which act as
electron transporters as they flow through the inner membrane space. In complex
I, electrons are passed from NADH to the electron transport chain, where they
flow through the remaining complexes. NADH is oxidized to NAD in this process.
Complex II oxidizes FADH, garnering still more electrons for the chain. At
complex III, no additional electrons enter the chain, but electrons from
complexes I and II flow through it. When electrons arrive at complex IV, they
are transferred to a molecule of oxygen. Since the oxygen gains
electrons, it is reduced to water.

While these oxidation and reduction reactions take place, another, connected
event occurs in the electron transport chain. The movement of electrons through
complexes I-IV causes protons (hydrogen atoms) to be pumped out of the
intermembrane space into the cell
cytosol. As a result, a net
negative charge (from the electrons) builds up in the matrix space while a net
positive charge (from the proton pumping) builds up in the intermembrane space.
This differential electrical charge establishes an electrochemical gradient. As
we will see in the next
section, it is
this gradient that drives ATP synthesis in oxidative phosphorylation.