CK2 alpha (also termed casein kinase II alpha ) is a serine-threonine protein kinase whose targets include many critical regulators of cellular growth. It is highly expressed in a lympho pro-liferative disease of cattle and in many human cancers. Overexpression of the CK2 alpha catalytic subunit in lymphocytes of transgenic mice leads to T cell lymphoma (1). The highest CK2 activity is found in mouse testicles and brain, followed by spleen, liver, lung, kidney and heart (2). The activity values were directly correlated with the protein expression level of the CK2alpha (catalytic subunit). The alpha subunit is only detected in brain and testicles. By contrast, Northern blot analyses of the CK2 alpha mRNA shows the strongest signals to be present in brain, liver, heart and lung. In kidney, spleen and testicles mRNAs is only weakly detectable. ICBP90, a transcription factor exhibiting antiapoptotic property, has several putative CK2 phosphorylation sites. ICBP90 is more efficiently phosphorylated by the free CK2 alpha subunit than by the heterotetrameric CK2 (alpha , beta) (3). Thus, CK2 alpha is an important regulator of the transcriptional activity of ICBP90 and therefore of the antiapoptotic properties of ICBP90.

Species

Human

Expression System

Baculovirus (Sf9 insect cells)

Applications

Apuri, Blocking, ELISA, Functional Assay

Application Note

107 nmol phosphate incorporated into casein per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).

Predicted Target Size(KDa)

70.0 kDa(note)

Form Supplied

Liquid

Purification Note

Purity was assessed by SDS-PAGE (≥90%) and by HPLC.

Concentration

0.1
mg/ml
(Please refer to the vial label for the specific concentration)