Hi all:
I'm about to generate my own TA-vector for PCR product cloning.
I'll digest pXcmkn12 with XcmI. But then I do not know whether I have to
dephosporylate in order to prevent religation.
Has anybody of you done that before ?
Did you use Boeringer's shrimp alkaline phosphatase (easier to deactivate
later) or any ordinary CIP ?
Cheers,
Markus