Our research interests focus on the molecular structure and generation models of double minutes (DMs) in human tumors. We applied array CGH, FISH, NimbleGen capture array and high-throughput sequencing to analyze DMs, constructed DMs structures, and completed the whole sequences of DMs genome. DMs are the hallmarks of extrachromosomal gene amplification related to tumorigenesis and tumor progression. Molecular structure research of DMs provides us not only the amplified oncogenes but also the underlying molecular mechanisms for DMs formation. Our study indicated that high-throughput sequencing would be a powerful tool for researching the structure of DMs in solid tumors.