An adsorptive stripping voltammetric method was developed for the determination of permethrin at single walled carbon nanotube modified glassy carbon electrode. The electrochemical reduction behavior of permethrin was studied by cyclic voltammetry and differential pulse voltammetry. Permethrin exhibited a single well defined reduction peak at -1.15 V vs. Ag/AgCl due to 2 e- irreversible reduction of the carbon – carbon double bond in acidic medium. The electrode and reaction conditions which yielded maximum peak current were established using differential pulse voltammetry. At the modified electrode, a linear response was obtained between peak current and concentration of permethrin over the range from 1.0 x 10-8 to 1.0 x 10-6 M with detection limit of 1.2 x 10-9 M. The practicability of the recommended method was verified by the determination of permethrin in spiked human urine samples.