1MICROBIAL ANALYSIS OF INDOOR AIR QUALITY AT A COMMUNITY COLLEGE Rona Silva Charles Havnar Biology Department Skyline College. San Bruno CA 94066 ABSTRACT According to the U.S. Environmental Protection Agency (EPA), the average American spends nearly 90 of his or her time indoors consequently, the EPA considers indoor air pollution a high priority health risk. Recurring outbreaks of respiratory illness in office workers have been described since 1970 these nonspecific illnesses became known as sick building syndrome and their causes attributed to a variety of microorganisms. At present, there are no uniform national standards for airborne bacteria and fungi. The purpose of this study was to enumerate airborne bacteria and fungi in indoor air at Skyline College. We examined 84 air samples (48 indoor samples and 36 outdoor samples) from four buildings between June and August 2004 using an MB2 air sampler. Culturable airborne bacterial concentrations in indoor air were equal to or lower than in outdoor air. The culturable airborne fungal concentrations in indoor air were 33 higher than those in outdoor air. The variety and number of microorganisms inside Building 1 was significantly higher than outdoors, which may indicate an internal source of contamination. The air filters in Building 1 were cultured to determine the microbial concentration. Nearly all tests revealed lt500 fungi/mm3. This could be indicative of the air filter's inability to trap smaller fungal and/or bacterial particles. We recommend strict adherence to the published schedule for replacing heat-ventilation filters in all buildings.RESULTS We examined 84 air samples (48 indoor samples and 36 outdoor samples) from four buildings at Skyline College. Indoor and outdoor humidity ranged from 40-60 during this study. The average number of fungi in indoor air was approximately 33 higher than outdoor air. The average number of bacteria in indoor air was approximately 43 lower than outdoor air (Figure 3). Biocontamination levels varied by building and increased with increased age of the buildings Building 1, the oldest at 35 years, showed the highest levels of both bacteria and fungi (Figure 4). Cultures obtained from indoor air samples taken with the MB2 air sampler failed to show large numbers of any single fungal or bacterial species (Figure 5). From outdoor air, one actinomycete bacterium and one Stachybotrys fungus were cultured (Figure 6). Both are found naturally in soil and indoor accumulations of these organisms have been shown to cause various adverse health effects (7, 12). Bacteria cultured included endospore-forming aerobes (Bacillus) and a variety of pigmented cocci (Figure 5b), but these bacteria are expected in air because they can withstand desiccation and ultraviolet radiation. Between 9-13 ? 107 fungal cfu/15cm3 were cultured from Building 1 rooftop intake filters in use for 3 months and 6 months (Figure 7). No bacteria were cultured from the filters. Rodac plates containing Sabouraud dextrose agar pressed directly onto the grates inside the Building 1 intake vent showed fungal counts gt100 cfu/47 mm2 (Figure 8) no fungi or bacteria grew on Rodac plates pressed against the vent walls. The filters concentrated airborne fungi the highest number of fungi was on the filters, and the lowest number, outdoors (Figure 9). a. Penicillium, sometimes associated with sick building syndrome (15), was the predominant fungus cultured.BACKGROUND Dirty heating ventilation air conditioner (HVAC) filters can contain up to 3,400 fungal cfu and 6,700 bacterial cfu per gram of dust (4). Fungi specifically, in indoor air, are increasingly being proposed as a cause of sick building syndrome. In buildings where microbes grow in the moist environments afforded by leaky pipes or roofs and decaying building material, the chances of experiencing adverse health effects ranging from mild allergies and colds to nasal bleeding and bronchitis increase (6). With the popularity of energy efficient building construction and less ventilation with outside air, the indoor environment has become an ideal setting for mold. Maintaining optimum indoor air quality therefore is of great importance. Although the U.S. Food and Drug Administration recognizes the health risks associated with indoor air pollution, at present, it cannot provide national health standards for air filter performance as research data on the relationship between air filtration and actual health improvement is lacking (9). To evaluate the relationship between airborne fungi and adverse health effects, fungi and their frequency in both indoor and outdoor air need to be known (14). Information obtained from air samples can assist in medical evaluations, determination of remediation, and assessment of health hazards. The purpose of this study was to enumerate airborne bacteria and fungi in indoor air at Skyline College, and to test the bacterial/fungal filtration performance of HV filters being used. Figure 7. Comparison of fungi in 3- and 6-month-in-use filters from Building 1. Bars show ranges from all samples. MATERIALS METHODS Figure 8. Fungi cultured on SDA from filter vents.Figure 9. Comparison of fungi along the path of air into Building 1. Collected microorganisms and inoculated culture media using the MicroBio MB2 (Spiral Biotech) impact sampler June-Aug. 2004. The MB2 is a statistical air sampler identified for use by the American Conference of Governmental Industrial Hygienists (2). (Figure 1, Table 1).DISCUSSION CONCLUSIONS Shelton (14) reports mean outdoor airborne fungal concentrations of 930 cfu/m3 and mean indoor concentrations of 300 cfu/m3 in the far west. The prevalence of microbes in outdoor air in San Mateo County is low because of prevailing westerly winds off the Pacific Ocean that move airborne particulates to the east. Moreover, remaining particulates are likely to precipitate with coastal fog. Only viable cells were counted in this study. However, it has been found that culture techniques may underestimate the bacterial burden of indoor air by as much as 90 (5). Additionally, foot traffic and vacuuming have been shown to increase fungal counts (3), and human activity in and around sampling sites occurred during indoor air sampling. Overall, Building 1 showed the highest counts and greatest diversity of microorganisms found the variety and number of microorganisms inside Building 1 was different than outdoors, which may indicate an internal source of contamination due to microbial growth in water damaged walls resulting from recurrent winter flooding. As many as 30 percent of the buildings in the developed world may have problems leading to occupant complaints and illness (1). Despite this and myriad health issues associated with poor quality indoor air, government regulation of air filters remains nonexistent. The American Society of Heating, Refrigeration, and Air Conditioning Engineers standards for HVAC filters are based on particle size (10). Our results indicate the Air Handler is filtering out larger particles, but is too porous to catch many of the smaller bacterial particles. Rodac plates pressed directly into the rooftop intake vents of Building 1 grew Penicillium and Rhizopus (Figure 8). Our study showed that outdoor fungal aerosols were at the 25th percentile in the United States (14), and indoor air biocontamination levels were within published recommendations (8, 11) at the 25th percentile for buildings in the United States (14). We recommend strict adherence to the published schedule for replacing heating/ventilation filters in all buildings possibly a change to pleated air filters that offer removal efficiencies well above 20, provide extended surface area allowing the capture of more particulates without causing air flow problems, and competitive costs with other filtration options (2) and periodic steam cleaning of the grates. Figure 3. Concentrations of airborne microorganisms at Skyline College. Bars show ranges from all samples. Figure 4. Comparison of airborne microorganisms by building. The buildings range from 11 years (Bldg 5) to 35 years (Bldg 1). Bars show ranges from all samples. Figure 1. Air samples were collected with the MB2, which collects airborne bacteria and fungal spores from air flowing at 100 liters/minute through a series of 1 mm diameter air inlets, onto an agar filled 47 mm contact plate. Sabouraud dextrose agar (SDA, Criterion) incubated at 20C for 5 to 7 days was used to culture fungi. Tryptic soy agar (TSA, Criterion) incubated at 35C for 2 days was used to culture bacteria.1cm ? 1cm and 2 cm ? 2 cm pieces of 3 and 6-month-old polyester Air Handler (manufacturer) 5W102 air filters used at the ventilation intake on the roof of Building 1 were vortexed in 99-ml aliquots of sterile water Jan-May 2005 (Figure 2).a. Fungi from 1m3 air collected on SDA plates. b. Nine species of bacteria including endospore-forming aerobes were collected from 0.5m3 of air on a TSA plate Figure 5. Microbes cultured from Building 1, stairwell, indoor air.REFERENCES 1. Akimenko, V. V. et al. 1986. "The 'sick' building syndrome," Proceedings of the Third International Conference on Indoor Air and Climate 687-97. 2. American Conference of Governmental Industrial Hygienists. 1989. Guidelines for the Assessment of Bioaerosols in the Indoor Environment. Cincinnati, OH American Conference of Governmental Industrial Hygienists. 3. Buttner, M. P. and L. G. Stetzenback. 1993. Monitoring airborne fungal spores in an experimental indoor environment to evaluate sampling methods and the effects of human activity on air sampling. Applied and Environmental Microbiology 59(1)219-236. 4. Hedge, Alan. 2005. Indoor Air Quality Biological Organisms. Http//ergo.human.cornell.edu/studentdownloads/DEA350pdfs/iaqbiol.pdf (15 April 2005). 5. Heidelberg, J. F. et al. 1997. Effect of aerosolization on culturability and viability of gram-negative bacteria. Applied and Environmental Microbiology 63(9)3585-3588. 6. Koskinen, O. M. et al. 1999. The relationship between moisture or mould observations in houses and the state of health of their occupants. European Respiratory Journal 141363-1367. 7. Kuhn, D. M. and M. A. Ghannoum. 2003. Indoor mold, toxigenic fungi, and Stachybotrys chartarum infectious disease perspective. Clinical Microbiology Reviews 16(1) 144-172. 8. Miller, J. D., et al. 2000. Air sampling results in relation to extent of fungal colonization of building materials in some water-damaged buildings. Indoor Air 10(3)146-151. 9. Nelson, H. S. et al. 1988. Recommendations for the use of residential air-cleaning devices in the treatment of allergic respiratory diseases. Journal of Allergy and Clinical Immunology 82(4)661-669 10. Air Filtration Systems. Http//www.afslasvegas.com/Tri-Dim/merv.htmlintro (29 February 2005). 11. OSHA. Technical Manual. 1992. U.S. Department of Labor. Occupational Safety and Health Administration TED 1-0.15A.. lthttp//www.osha.gov/dts/osta/otm/otm_toc.htmlgt 12. Paannen, A. R. et al. 2000. Inhibition of human NK cell function by valinomycin, a toxin from Streptomyces griseus in indoor air. Infection and Immunity 68165-169. 13. Ribes, J.A. 2000. Zygomycetes in human disease. Clinical Microbiology Reviews Apr13 (2)236-301. 14. Shelton, B.G., et al. 2002. Profiles of airborne fungi in buildings and outdoor environments in the United States. Applied and Environmental Microbiology 68(4)1743-1753. 15. Wilson, Stephen et al. 2004. Culturability and Toxicity of Sick Building Syndrome-Related Fungi Over Time. Journal of Occupational and Environmental Hygiene 1(8)500-504. Aseptically made serial dilutions of the filter slurry ranging from 110-1107.Performed standard plate counts on SDA and nutrient agar (Criterion, NA).SDA plates were incubated at 25C for 5-7 days NA plates at 35C.a. Actinomycete bacteria from 1m3 of outdoor air (Bldg 1 roof).b. Stachybotrys cultured from 1m3 of air outside Bldg 5. The normal habitat of Stachybotrys is soil. Soil excavation may have released spores into the air on this sampling day.Figure 6. Microbes collected from outside air.Rodac plates containing SDA (Criterion) were used to culture fungi from inside the Building 1 intake vents May-July 2005.ACKNOWLEDGEMENTS We thank Dr. Christine Case for her advice and patient encouragement throughout this process Patricia Carter and the other Biology laboratory technicians for their timely supply of materials, and helpful suggestions Megan Abadie, Tim Ashton, Richard Davis, Myra Grace Gray, Cao Hoang, Arran Phipps, and Sasha Rose for collecting samples and Tony Vassalle and Tony Gulli for taking us through the vents.Figure 2. Air intake filters from Building 1 were tested for bacteria and fungi. Rhizopus gets caught in the filter

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