Brilliant Violet 421/ Alexa Fluor 405 Filter Set 49027

Chroma’s 49000 series of single band sets features our highest throughput sputter coatings (ET) which achieve the highest transmission levels for interference filters.

These filters have very steep transitions from peak transmission to high optical density to best exploit the spectral properties of modern fluorochromes with short Stokes shifts. This is because steep transitions allow for very little spectral separation between excitation and emission bands. These filters offer the greatest hardness and durability against environmental factors such as high heat and humidity, and are covered by Chroma’s lifetime warranty. All ET filters sets provide zero-pixel-shift image registration* between filter sets, at no additional cost.

*It is important to note that filters are only one factor in achieving perfect image registration in imaging systems. All other components and devices must also be in register.

This narrow-band filter set is intended for very bright fluorochromes such as BD Horizon Brilliant™ Violet 421. It is sufficiently narrow to allow spectral separation from cyan fluors such as BD Horizon Brilliant™ Violet 480, cyan fluorescent proteins and Aqua FISH probes. This set is also useful for detecting DAPI. DAPI will appear violet instead of blue.

Typical Application(s): Widefield Microscopy

Microscopes: Filter cube mounting options are available when adding to cart.