[Histonet] rapid procedure for methylmetacrylate

From:

Gayle Callis

If the tissue is soft, just cut back on the times in your solutions.
You can process your tissues up through 1 change of xylene and 1
change Clearite 3 on your tissue processor - nice an easy, DO NOT GO
THROUGH THE parafffin stations. If the tissues are small, 1 hour per
station is acceptable. 70%, 80%, 95% x 2, 100% x 2, Xylene X 2 OR
Xylene x 1, Clearite 3 X 1 - these are number of changes. This
eliminates tedious hand processing.
Start infiltration with MMA mixtures (2 mixtures) 4 hours each with
last change overnight in a final mixture to insure proper
infiltration, then embed, let sit overnight at RT, then polymerize
in a 37C waterbath (not an oven!) I will be happy to send via private
attachment, a protocol by Diane Sterchi for MMA mixtures, etc. She
did short schedules for MMA with great success. This still is not a
truly rapid protocol, but probably faster than what you use now. Her
MMA mixtures infiltrated nicely, were not overly thick and polymerized
like a dream.
At 08:26 AM 8/6/2004, you wrote:
Hi everyone
i am using a methylmetacrylate embedding procedure on thin and soft
resconstructed tissue on titane biomaterial, this protocole i found
is adapted for bone specimen with long time for dehydratation (1
day for each alcohol) and impregnation (2 days for each bath).
Could you give me please a successfull rapid procedure for
embedding this type of tissue in MMA ?
Any advices on reducing times are welcome.
thanking you in advance.
Myriam baali
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
References
1. http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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