Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Details

Applications

Approved:

ELISA

ELISPOT

Specificity and Use

Immunogen:

IL1A / IL-1 Alpha antibody was raised against mouse IL1A

Usage:

This polyclonal antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse interleukin-1&alpha; (IL-1&alpha;) in combination with the purified ALF-161 (LS-C107129) antibody for capture and recombinant mouse IL-1&alpha; as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 ug/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Description: IL1A / IL-1 Alpha is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.