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The game began with Jeff Probst asking everyone to say their name and their jobs. Most of the contestants were truthful. Crystal neglected to mention she was an Olympic athlete because it might make her a bigger target. Jeff then asked the oldest man and oldest women (Bob and Gillian) to pick a mat and go over there, where they would pick the tribes. Bob beat Gillian in rock-paper-scissors so he began picking first. He picked "baldy" (not knowing Ace's name), so Ace joined the Kota tribe. Gillian picked Crystal. Ace picked Sugar, saying it wouldn't be appropriate for a photographer not to have a model. Crystal picked Susie, in order to have all three moms on the same tribe, thinking that moms have to stick together. It was an odd choice, as Charlie noted in his confessional. Sugar picked Marcus next while Susie picked Matty. In the next round, Marcus chose Charlie (much to Charlie's excitement) and Matty chose Randy. The next choices were Charlie picking Paloma and Randy choosing Dan. Then Paloma chose Kelly and Dan chose GC. Kelly chose Jacquie and GC chose Ken. Jacquie chose Corinne, completing the Kota tribe. Michelle tried her best not to seem visibly upset she was picked last, and then was selected by default to complete the Fang tribe.
Crystal and Gillian slow down the tribe in a challenge, causing a loss for the Fang tribe.

Challenge:One For All
The tribes would have to race across the plain and up a hill. The first castaway from each tribe to the top of the hill would win an Individual Immunity necklace that would only be good at their tribe's first Tribal Council. The first entire tribe to the top of the hill would win the reward.Reward: An extra bag of corn and beansWinners:G.C. Brown and
Marcus Lehman(immunity);
Kota(reward)

Challenge:Temptation Valley
Six tribe members would be belted together and race through a swamp, over a net wall, and under and over a series of hitching posts. These six would then have to dig up three bags of puzzle pieces and deliver them to the three remaining tribe members. These three would then assemble the puzzle pieces to win the challenge.Reward: Flint and ImmunityWinner:Kota

My vote for you is simply based on the fact that so many people want to be you so desperately and it looks like you haven't been from the moment you got here. And it's been a drain for you and I think for everybody else. Good luck to you and sorry about it but I don't think you want to be here, and with your whole attitude, I don't think you should be.

Due to the extended 2 hours premiere episode, Michelle's final words (aka. exit confessional) and all the votes from that Tribal Council that are supposed to be revealed at the end of the episode were cut out. However, it was made available for viewers to watch on the CBS website after the episode was aired.