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Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age

14 weeks gestation

Gender

male

Ethnicity

Caucasian

Applications

These cells are provided to be used as feeder cells to support the growth of other cells.

Clinical Data

14 weeks gestation

Caucasian

male

Comments

These cells have been growth-arrested by irradiation with 10,000 rads and will begin to deteriorate 2 weeks after plating. We recommend that you do not keep the cells in culture for longer than 2 weeks.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Culture Conditions

Temperature: 37°C

Atmosphere: Air, 95%; carbon dioxide (CO2), 5%

Name of Depositor

JP Jacobs

Permits

These permits may be required for shipping this product:

Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.