Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.

Involvement in disease

Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.

ab8295 staining Cardiac Troponin T in the human iPS cell derived from cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% triton X in PBS and blocked with 1% BSA for 18 hours at 4°C. Samples were incubated with primary antibody (1/100 in 1% BSA/PBS) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

Ab8295 staining human normal heart. Staining is localised to the cytoplasm.Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

I am happy to confirm that we do have antibody pairs validated for sandwich ELISA for Troponin T, C and I. We do not have tested troponin proteins as standard. For the Troponin T,I have also double checked with the source of the antibodies, and they do have a protein which they can recommend. I am currently checking with my colleagues if we can add it to our catalog. However we do have the individual standard proteins or also a recomplexed protein, which could be tested also as a standard. Please let me know what you think. Indeed, it could be that the recomplexed protein is more stable and less prone to protein degradation and you could use it for all the three ELISAs. potential standard complex: ab94712 https://www.abcam.com/index.html?datasheet=94712 (or use the following: https://www.abcam.com/index.html?datasheet=94712).

For all the proteins or for the complex, we are happy to provide you with our testing discount code as well. Please let me know whether you are interested and I am happy to send you the detailed description of this offer (you would buy the protein to the normal price, test it in ELISA, give us the feedback and then you will get another protein (or other product) for the same price for free. So basically the offer is 100% however only on a second product - for more information, please see also https://www.abcam.com/test-vial)

Unfortunately we do not have any ELISA kits for the moment against the cardiac Troponins. We are however looking into adding them to our catalog within a few months.

I hope this information is helpful. Please do not hesitate to contact me again, should you have any additional questions or should you like to receive the testing discount.

I apologize for the confusion regarding this antibody's storage conditions. We previously recommended storage at 4C for up to one year because that is how the antibody was stored by the lab, and we knew that this temperature was suitable for this product. However, we have recently determined that the antibody can also be stored at -20C, and we recommend this now because theortetically the antibody will be viable for a longer period of time if it is frozen compared to 4C. If you have stored the antibody at 4C, youshould not be concerned, and our Abpromise guarantee still applies to this product.

I hope that this information will be useful, but please let me know if you have any further questions and I'll be happy to help.

The two antibodies will work together. The protein we do not know whether it will work in ELISA.

This is why we could give you a testing discount on the protein. This means, you would buy the protein to the normal price, test it in ELISA, give us the feedback and then you will get another protein (or other product) for the same price for free. So basically the offer is 100% however only on a second product.

The details of the offer can also be found in the last email, where I explained the offer step by step.

Please let me know if this is not clear and if you would wish to clarify some other points.

I hope this is helpful. Please do not hesitate to contact us for any additional information.

I am happy to confirm that the ab8295 and the ab8296 are perfectly well choosen together. They will work as a sandwich pair in ELISA.

The ab9937, which you would use as a standard, has not yet been tested in ELISA. It might well work, depending on its conformation. As however we have not assessed experimentally whether the ab9937 can be used in ELISA as a standard, I can offer a discount off a future purchase. If you buyab9937 now, test it inELISA as a standard for ab8295 and ab8296and submit feedback to us in the form of an Abreview, you would receive a discount worht the value of this protein. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab9937 in ELISA. I will then send a discount code. This code must be issued before purchasing ab9937 so please wait for my reply before ordering.

2. Purchase ab9937 either by phone, fax, or online (www.abcam.com).

3. Test it in ELISA.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any product ordered and the discount code is valid for 4 months after issue. Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifab9937 turns out to be unsuitable for ELISA, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.