We have studied regulatory nascent chains such as B. subtilis MifM and E. coli SecM. We collaborated with structural biologists to determine the structure of the MifM-ribosome complexes. Our genetic analysis revealed that MifM interacts with the ribosome, extensively, including the ribosomal surface to arrest translation. We also found that the release of the elongation arrest of MifM depends on the hydrophilic cavity of the membrane protein insertion factor YidC.

Academic Significance and Societal Importance of the Research Achievements