The Full Human Immune System Mouse Models: in vivo pharmacology fee-for-services

TransCure bioServices has developed a strong expertise, data set driven for the 100% functional reconstitution of a full human immune system in severely immunodeficient mouse strains (NOG, BRG, BRG-S, NCG) via engraftment of human umbilical cord blood CD34+ stem cells.

Full Human Immune System Reconstitution

Engraftment and differentiation of human stem cells

High And Stable Humanization Rate

Flow Cytometry Characterization

Human Cells in Peripheral Blood of hu-Mice

Human Cell type

Expressed In HIS

Marker

Leucocytes

✓

CD45

T-Lymphocytes

✓

CD3, CD4, CD8, CD25

B-Lymphocytes

✓

CD19

NK cells

✓

CD16, CD56

Dendritic cells

✓

CD1c, CD303,CD141, CD11c

Monocytes

✓

CD14

Macrophages

✓

CD11b

Activated T and/or NK cells

✓

HLA-DR, CD69

Human Cytokines in hu-Mice

Cytokine

Expressed In HIS

Method of Detection

huTNF-α

✓

IHC

IFN-

✓

FC, RT-PCR

IL-2

✓

FC

IL-4

✓

FC

IL-17

✓

FC, RT-PCR

IL-23

✓

RT-PCR

In details, following chemoablation, the young animals are engrafted with human CD34+ Hematopoietic Stem Cells (HSC) isolated from human cord blood in our BSL-2 animal facility. 14 weeks post-CD34+ engraftment, the rate of the full human immune system reconstitution is assessed and the human immune cell population is monitored by state-of-the-art flow cytometry.